# Patent

## Title
Methods and compositions for inhibiting or reducing hair loss, acne, rosacea, prostate cancer, and BPH

## Abstract

This invention provides methods of treating androgenetic alopecia (AGA), acne, rosacea, prostate cancer, and benign prostatic hypertrophy (BPH), comprising the step of contacting a subject with a compound or composition capable of decreasing prostaglandin D2 (PGD2) level or activity, a downstream signaling or receptor pathway thereof, or prostaglandin D2 synthase level or activity; methods of stimulating hair growth, comprising the step of contacting a subject with a compound or composition capable of increasing or decreasing the activity or level of a target gene of the present invention, or with a protein product of the target gene or an analog or mimetic thereof; and methods of testing for AGA and evaluating therapeutic methods thereof, comprising measuring PGD2 levels.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a National Phase Application of PCT International Application No. PCT/US07/14031, International Filing Date Dec. 27, 2007, claiming priority of U.S. Provisional Patent Applications 60/814,041, filed Jun. 16, 2006 and 60/845,161, filed Sep. 18, 2006, all which are incorporated herein by reference in their entirety.


FIELD OF INVENTION
This invention provides methods of treating androgenetic alopecia (AGA), acne, rosacea, prostate cancer, and benign prostatic hypertrophy (BPH), comprising the step of contacting a subject with a compound or composition capable of decreasing prostaglandin D2 (PGD2) level or activity, a downstream signaling or receptor pathway thereof, or prostaglandin D2 synthase level or activity; methods of stimulating hair growth, comprising the step of contacting a subject with a compound or composition capable of increasing or decreasing the activity or level of a target gene of the present invention, or with a protein product of the target gene or an analogue or mimetic thereof; and methods of testing for AGA and evaluating therapeutic methods thereof, comprising measuring PGD2 levels.
BACKGROUND OF THE INVENTION
In normal scalp, hair follicles (HF) constantly cycle between a growing stage (anagen), an involutional stage (catagen), and then a dormant stage (telogen). The length of the hair is determined by the duration of anagen. Thus, scalp follicles stay in anagen for 2-7 years, while non-scalp follicles typically remain in anagen for much shorter times. The caliber of the hair shaft and the cycling of the follicle are thought to be under control of the dermal papilla, a cluster of inductive mesenchymal-derived cells located at the base of the follicle. Anagen onset appears to be triggered by mesenchymal-epithelial interactions between the dermal papilla cells and nearby hair follicle stem cells in the bulge, which is located at or near the insertion of the arrector pili muscle. Bulge cell progeny generate the new lower anagen hair follicle at anagen onset and contribute cells to the epidermis after wounding.
Androgenetic alopecia (AGA) remains enigmatic. Often, AGA is characterized by conversion of large “terminal” follicles into “miniaturized” follicles that resemble the vellus follicles of the prepubescent face. AGA often results in an increase in the proportion of hair follicles in telogen at the expense of follicles in anagen. Often, mild fibrosis replaces follicles in long-standing alopecia. Hypotheses to explain AGA include hormonal, genetic, and inflammatory insults.
SUMMARY OF THE INVENTION
This invention provides methods of treating androgenetic alopecia (AGA), acne, rosacea, prostate cancer, and benign prostatic hypertrophy (BPH), comprising the step of contacting a subject with a compound or composition capable of decreasing prostaglandin D2 (PGD2) level or activity, a downstream signaling or receptor pathway thereof, or prostaglandin D2 synthase level or activity; methods of stimulating hair growth, comprising the step of contacting a subject with a compound or composition capable of increasing or decreasing the activity or level of a target gene of the present invention, or with a protein product of the target gene or an analogue or mimetic thereof; and methods of testing for AGA and evaluating therapeutic methods thereof, comprising measuring PGD2 levels.
In one embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject with a compound or composition capable of inhibiting a prostaglandin D2 activity, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is caused by an AGA. In another embodiment the compound or composition inhibits the prostaglandin D2 activity in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject with a compound or composition capable of inhibiting a signaling or receptor pathway downstream of prostaglandin D2, thereby treating baldness in a scalp of a subject. In another embodiment; the baldness is caused by an AGA. In another embodiment, the compound or composition inhibits the signaling pathway in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject with a compound or composition capable of decreasing a level of a prostaglandin D2, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is caused by an AGA. In another embodiment, the compound or composition decreases the prostaglandin D2 level in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject with a compound or composition capable of inhibiting an activity of a prostaglandin D2 synthase enzyme, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is caused by an AGA. In another embodiment, the compound or composition inhibits the prostaglandin D2 synthase activity in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject with a compound or composition capable of decreasing a level of a prostaglandin D2 synthase enzyme, thereby treating baldness in a scalp of a subject.
In another embodiment, the baldness is caused by an AGA. In another embodiment, the compound or composition decreases the prostaglandin D2 synthase level in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of increasing an activity of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from FGF18, GPRC5D, GPR49, LRRC15, Serpin A, and CDT6. In another embodiment, the gene is selected from BMP2, LHX2, THBS1, MYCN, NR4A2, MEST, TM4SF1, CRLF1, TNFRSF12A, SELENBP1, GPR161, HEPH, FZD7, and CLIC4. In another embodiment, the compound or composition increases the target gene activity in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of increasing an expression of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from FGF18, GPRC5D, GPR49, LRRC15, Serpin A, and CDT6. In another embodiment, the gene is selected from BMP2, LHX2, THBS1, MYCN, NR4A2, MEST, TM4SF1, CRLF1, TNFRSF12A, SELENBP1, GPR161, HEPH, FZD7, and CLIC4. In another embodiment, the compound or composition increases the target gene expression in the target scalp or skin. In another embodiment, the compound or composition increases the level of a protein encoded by the target gene in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a recombinant protein product of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, a mimetic or analogue of the protein product is administered. In another embodiment, the compound or composition increases the protein product concentration in the target scalp or skin. In another embodiment, the protein product is FGF18. In another embodiment, the protein product is a product of any target gene selected from GPRC5D, GPR49, LRRC15, Serpin A, and CDT6. In another embodiment, the gene is selected from BMP2, LHX2, THBS1, MYCN, NR4A2, MEST, TM4SF1, CRLF1, TNFRSF12A, SELENBP1, GPR161, HEPH, FZD7, and CLIC4. In another embodiment, the protein product is a product of any target gene of the present invention. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of decreasing an activity of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, PTGD2, and CORIN. In another embodiment, the compound or composition decreases the target gene activity in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of decreasing an expression of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, PTGD2, and CORIN. In another embodiment, the compound or composition decreases the target gene expression in the target scalp or skin. In another embodiment, the compound or composition decreases the level of a protein encoded by the target gene in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a prostaglandin D2 (PGD2) in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 metabolite in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 metabolite in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in the scalp of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in the scalp of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in a body fluid of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in a body fluid of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 or a metabolite thereof in a body fluid of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 or metabolite thereof in the body fluid, wherein, if the second level is appreciably decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, the method comprising the steps of (a) isolating an alpha-6 integrinhigh CD200high cell population from a haired area of the subject; and (b) contacting a bald or hair-deficient region of the subject with the alpha-6 integrinhigh CD200high cell population, thereby stimulating a hair growth in a subject.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of increasing a level of a CD200 protein, thereby stimulating a hair growth in a subject.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a recombinant CD200 protein, thereby stimulating a hair growth in a subject. In another embodiment, a mimetic or analogue of a CD200 protein is administered. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject with a compound or composition capable of decreasing an activity of a CD200 protein, thereby stimulating a hair growth in a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating prostate cancer or BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the prostate gland, thereby treating prostate cancer or BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer or BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the prostate gland, thereby treating prostate cancer or BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer or BPH in a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the prostate gland, thereby treating prostate cancer or BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer or BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the prostate gland, thereby treating prostate cancer or BPH in a subject.
In another embodiment, the present invention provides a method of predicting the development of AGA in a subject, comprising the step of measuring an amount of PGD2 in the skin or scalp of the subject. In another embodiment, an amount of a PGD2 metabolite is measured. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and an inhibitor of prostaglandin D2 activity. In another embodiment, the pharmaceutical composition further comprises a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and an downregulator of prostaglandin D2 levels. In another embodiment, the pharmaceutical composition further comprises a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising an inhibitor of prostaglandin D2 activity and a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1: A. Histology of AGA and Immunohistology of KRT15 in bald scalp. Hematoxylin and eosin stained tissue sections of haired (A) and bald (B) scalp photographed at same magnification exhibit large terminal hair follicle and miniaturized follicle. Note anagen follicle extending to subcutaneous fat in A and only to level of arrector pili muscle (apm) in B. (C) Immunohistology of sections of terminal hair follicle (thf) and miniaturized hair follicle (mhf), both staining for the bulge stem cell marker KRT15 (brown stain, arrows), indicating presence of stem cell compartment in bald scalp. Staining in superficial epidermis (epi) is non-specific. sg, sebaceous gland. (D-E). Scanning electron microscopy of showing hair follicle miniaturization in AGA (E) compared to haired scalp (D).
FIGS. 2A-D FACS analysis of keratinocyte isolated from scalp indicates stem cell numbers are similar in paired bald and haired scalp samples, but suprabasal cell numbers are decreased within the stem cell compartment of bald scalp. A) Lack of staining from isotype antibodies, yet virtually complete staining with an anti-actin antibody as a control for permeabilization. Histogram with numbers of cells plotted as a percentage on the y axis, and intensity of staining on the x axis. B) KRT15 and FST co-localize. Pseudo-color plot of KRT15 staining intensity vs. FST staining intensity. Each point represents a cell counted, and the intensity of color is an indication of higher numbers of cells. C) Similar proportion of KRT15+/alpha-6 integrin+ cells in bald and haired scalp. Bald scalp cells (red in the original; middle panels) were overlayed onto haired cells (blue in the original; left panels), as shown in the overlay, which has bald cells in the foreground and haired cells in the background (right panels). Although the red bald cells mostly eclipsed blue haired cells, a conspicuously higher number of blue cells were evident in the stem cell positive alpha-6 integrin-quadrant (lower right; see FIGS. 2E-H). Insets: Percentages of each population. H) FST+ alpha-6 integrin+ compartments in haired and bald scalp. Pseudo-color plots (left and middle panel) and overlay (right panel) were prepared as in (G).
FIG. 2E-H. Grayscale version of FIG. 2A-D. Red appears as gray, and blue appears a black. FIG. 2I-J. Image showing the blue color only from the right panels of FIG. 2C-D.
FIGS. 3A-E. 251 genes with altered expression in bald vs. haired scalp, ranked by statistical significance. FIG. 3A.1-FIG. 3A.13. Overview. FIG 3B.1-FIG. 3B.7. Raw data. FIG 3C.1-FIG. 3C.8. Additional information regarding the transcripts. FIG. 3D.1-FIG. 3D.15. Additional information and reference numbers from other databases. FIG. 3E.1-FIG. 3E.23. More information about transcripts. Sequences were from genome version July 2003 (NCBI 34).
FIG. 4. Summary of haired and bald scalp gene expression profiles. A) Average correlation coefficients of haired vs. haired, bald vs. bald and haired vs. bald with standard errors. B) Gene clustering algorithm applied to the group of 10 samples, for the 250 genes with the most statistically significant differential expression. C) Genes whose averages are higher in the haired scalp (H) follow this pattern for all subjects (left); and genes whose averages are lower in the bald scalp (B) follow this pattern for all subjects (right). Depicted are discontinuous graphs (gene expression level: y axis; patient samples grouped as pairs along the x axis) for the 250 genes. First sample in each pair (beginning of line) is the haired scalp and second sample (end of line) is the bald scalp. D) Gene Ontology classifications for gene function ordered by numbers of probe sets in each category with p values.
FIG. 5. Specific gene expression changes in haired vs. bald scalp of selected genes. A) AR, 3beta-hydroxysteroid dehydrogenase (3 beta-HSD), and immune-related genes were significantly up-regulated in bald scalp. Genes enriched in the haired scalp were assigned positive values and calculated as haired/bald; genes enriched in the bald scalp were given negative values and calculated as bald/haired. Fold changes for probe sets corresponding to genes and p values as determined by 3-way ANOVA are listed. B) Additional genes not previously identified in human HF and shown herein to be upregulated in haired scalp.
FIG. 6. In situ hybridization showing expression of genes not previously described in human HF and shown herein to be enriched in haired scalp. Left: transverse section; right: horizontal or oblique section. A) LRRC15 is present in Huxley's layer and the cuticle layer of the inner root sheath (IRS). B) Serpin A is expressed in the companion layer (CL). C) GPR49 (LGF5, HG38) is expressed in the outer root sheath (ORS). D) CDT6 is expressed in the ORS. E) GPRC5D is expressed in the IRS and precortical (PC) area of the hair. F-G) FGF18 is expressed in the IRS, CL, matrix (M), and suprabasal cells of the bulge region in the isthmus between the arrector pili muscle (apm) and sebaceous gland (sg).
FIG. 7. A. Microarray analysis reveals statistically significant elevation of L-PGDS type in bald scalp compared to haired scalp of men with male pattern baldness. Each of 3 probe sets, all specific to the L-PGDS mRNA, shows an elevation of at least 3-fold (see Experimental Details Section, Example 1). B. PGD2 is elevated in all bald scalp. C. Mass spectrometry measurements of PGD2, PGE2, and PGF2a (first, second, and third bar, respectively) in haired vs. bald scalp. D. Quantitative RT-PCTR of L-PGDS.
FIG. 8. Primers used to generate in situ probes shown in FIG. 6.
FIGS. 9A-F. Haired scalp contains a population of CD200high alpha-6 integrinhigh cells that are lacking in bald scalp. FIG. 9A. Original figure. FIG. 9B. Figure converted to grayscale. Blue appears as black, and red appears as gray.
FIG. 10. A. 46 day-old mouse exhibiting baldness (alopecia) at the site of PGD2 application on the central back. Note area of hair regrowth at the edge of treated area. B. PGD2-treated and control mice at a 121-day timepoint.
FIGS. 11A-D. FIG. 11A. Hair follicle phenotype of normal and K14-COX2 mice. FIG. 11B. L-PGDS staining in anagen dermal papilla. Wild type adult mice were stained with antibodies against L-PGDS (red in original color figure) and DAPI (nuclei; blue in original color figure). The Red center in hair follicle is dermal papillae. FIG. 11C. Figure from FIG. 11B, with red fluorescence only depicted. FIG. 11D. Blue fluorescence only depicted.
FIG. 12. PGD2 skin levels in normal and K14-COX2 mice.
FIGS. 13A-B. Additional genes enriched in haired and bald scalp. FIG. 13A.1-FIG 13A.4. Overview. FIG. 13B.1-FIG. 13B.4. Additional information. “Unigene” refers to UniGene annotation for the Affymetrix chip.



DETAILED DESCRIPTION OF THE INVENTION
This invention provides methods of treating androgenetic alopecia (AGA), acne, rosacea, prostate cancer, and benign prostatic hypertrophy (BPH), comprising the step of contacting a subject with a compound or composition capable of decreasing prostaglandin D2 (PGD2) level or activity, a downstream signaling or receptor pathway thereof, or prostaglandin D2 synthase level or activity; methods of stimulating hair growth, comprising the step of contacting a subject with a compound or composition capable of increasing or decreasing the activity or level of a target gene of the present invention, or with a protein product of the target gene or an analogue or mimetic thereof; and methods of testing for AGA and evaluating therapeutic methods thereof, comprising measuring PGD2 levels.
In one embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject or the scalp thereof with a compound or composition capable of inhibiting a prostaglandin D2 activity, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment the compound or composition inhibits the prostaglandin D2 activity in the target scalp. Each possibility represents a separate embodiment of the present invention.
The AGA that is treated by a method of the present invention is, in another embodiment, a male-pattern baldness. In another embodiment, the AGA is a female-pattern baldness. In another embodiment, the AGA is any
In another embodiment, the prostaglandin D2 activity is a stimulation of a CRTH2 receptor.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the scalp with a compound or composition capable of inhibiting a signaling or receptor pathway downstream of prostaglandin D2, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment, the compound or composition inhibits the signaling pathway in the target scalp.
As provided herein, prostaglandin D2 activity is enriched in bald scalp. In another embodiment, decreasing prostaglandin D2 activity or levels inhibits a mechanism underlying baldness. As provided herein, prostaglandin D2 activity is enriched in bald scalp. In another embodiment, decreasing prostaglandin D2 activity or levels reverses a mechanism underlying baldness. Prostaglandin D2 levels can be decreased, in another embodiment, by inhibiting one of the synthetic pathways leading to its production. Each possibility represents a separate embodiment of the present invention.
“Scalp” in another embodiment, refers to the skin of the top of the head. In another embodiment, the term further includes the underlying connective tissue. In another embodiment, the term further includes the aponeurosis (galea aponeurotica). In another embodiment, the term further includes the underlying loose connective tissue. In another embodiment, the term further includes the pericranium (periosteum). In another embodiment, the term further includes the hair shaft. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a fluid derived from the scalp is utilized in a method of the present invention. In another embodiment, a fluid derived from the scalp known in the art is utilized. In another embodiment, the fluid is obtained using fine needle aspiration. In another embodiment, any other suitable method known in the art is utilized. In another embodiment, a hair shaft is utilized. Each possibility represents a separate embodiment of the present invention.
The signaling pathway that is inhibited in a method of the present invention is, in another embodiment, an adenosine A2a pathway. In another embodiment, the signaling pathway is any other signaling pathway known in the art.
In another embodiment, the signaling pathway is a prostaglandin J2 (PGJ2) pathway. In another embodiment, the pathway is mediated by a metabolite of PGJ2. The PGJ2 metabolite is, in another embodiment, any PGJ2 metabolite known in the art. In another embodiment, the signaling pathway is a prostaglandin F2 (PGF2) pathway. In another embodiment, the signaling pathway is a prostaglandin F2-alpha pathway. In another embodiment, the pathway is mediated by a metabolite of PGF2. In another embodiment, the pathway is mediated by a metabolite of PGF2-alpha. The PGF2 metabolite is, in another embodiment, any PGF2 metabolite known in the art. In another embodiment, the signaling pathway is a 15-deoxy-delta12,14-PGJ2-mediated pathway. In another embodiment, the signaling pathway is a delta12-PGJ2-mediated pathway. In another embodiment, the signaling pathway is a 9 alpha,11 beta-PGF2-mediated pathway. In another embodiment, the signaling pathway is a 9 alpha,11 beta-2,3-dinor-PGF2-mediated pathway. In another embodiment, the signaling pathway is a prostacyclin-mediated pathway. In another embodiment, the signaling pathway modulates activity of a peroxisome proliferator-activated receptor (PPAR) transcription factor. In another embodiment, the PPAR is a PPAR gamma-1. In another embodiment, the PPAR is a PPAR gamma-2. In another embodiment, the PPAR is a PPAR delta. In another embodiment, the PPAR is any other WAR known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the PGJ2 pathway is mediated by COMP (catechol-O-methyltransferase). In another embodiment, the PGJ2 pathway is mediated by a proteasome (prosome, macropain) 26S subunit (a.k.a. PSMD1). In another embodiment, the PGJ2 pathway is mediated by a SQSTM1 (sequestosome 1). Each possibility represents another embodiment of the present invention.
In another embodiment, the PGF2 pathway is mediated by a PGF2 receptor. In another embodiment, the PGF2 receptor is prostaglandin F receptor (FP) (a.k.a. PTGFR). Each possibility represents another embodiment of the present invention.
In another embodiment, the target of a method or composition of the present invention is a prostaglandin E receptor 2 (subtype EP2) (a.k.a. PTGER2) enzyme. In another embodiment, the target is a prostaglandin E receptor 1 (subtype EP1) (a.k.a. PTGER1) enzyme. Each possibility represents another embodiment of the present invention.
In another embodiment, the signaling pathway is mediated by a prostaglandin (PG) D receptor (DP receptor). In another embodiment, the signaling pathway is mediated by a DP-1 receptor. In another embodiment, the signaling pathway is mediated by a CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells)/DP2 receptor. In another embodiment, the signaling pathway is mediated by a non-canonical signal transduction mediator.
In another embodiment, the signaling pathway includes a DP receptor. In another embodiment, the signaling pathway includes a DP-1 receptor. In another embodiment, the signaling pathway includes a CRTH2/DP2 receptor. In another embodiment, the signaling pathway includes a non-canonical signal transduction mediator.
In another embodiment, the signaling pathway is a DP receptor pathway. In another embodiment, the signaling pathway is a DP-1 receptor pathway. In another embodiment, the signaling pathway is a CRTH2/DP2, receptor pathway. In another embodiment, the signaling pathway is a non-canonical signal transduction mediator pathway.
In another embodiment, the signaling pathway inhibited by methods and compositions of the present invention is any other signaling pathway mediated by a PGD2 receptor. In another embodiment, the signaling pathway any other signaling pathway mediated by a receptor for a PGD2 metabolite. In another embodiment, the signaling pathway is any other signaling or receptor pathway downstream of a PGD2 receptor. In another embodiment, the signaling pathway any other signaling or receptor pathway downstream of a receptor for a PGD2 metabolite. In another embodiment, the receptor is a non-canonical receptor.
Each signaling pathway represents a separate embodiment of the present invention.
The adenosine A2a pathway antagonist utilized in the present invention is, in another embodiment, KF17837.
In another embodiment, the adenosine A2a pathway antagonist is (−)-(11S,2′R)-.alpha.-piperidinyl-2,8-bis(trifluoromethyl)-4-quinolinemethanol. In another embodiment, the adenosine A2a pathway antagonist is (+)-(11R,2′S).alpha.-piperidinyl-2,8-bis(trifluoromethyl)-4-quinolinemethanol.
In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:




In another embodiment, the adenosine A2a pathway antagonist has the formula:




    wherein:
    R.sup.1 is —NR′R′′,
    
        wherein R′ and R′′ are independently selected from the group consisting of:
        
            lower alkyl, —(CH.sub.2).sub.n--C(O)NR.sup.aR.sup.b, —(CH.sub.2).sub.n-heteroaryl, —(CH.sub.2).sub.n-aryl, —(CH.sub.2).sub.n--CN, —(CH.sub.2).sub.n--O-lower alkyl or —(CH.sub.2).sub.n-cycloalkyl, or
        
        
        and R′ and R′′ form together with the N-atom a five or six-membered non-aromatic ring, containing no or one additional heteroatom selected from the group O and S, said ring being unsubstituted or substituted by one or two substituents, selected from the group consisting of lower alkyl, —C(O)NR.sup.aR.sup.b or —(CH.sub.2).sub.n--O-lower alkyl; and
        R.sup.aR.sup.b are independently from each other hydrogen or lower alkyl;
    
    
    R.sup.2 is aryl or heteroaryl, unsubstituted or substituted by lower alkyl or halogen; and
    n is 0, 1, 2 or 3.


In another embodiment, the adenosine A2a pathway antagonist is [8-Amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-piperidin-1-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]- -pyrrolidin-1-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]- -(2-methyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is 1-[8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridine-6-carbonyl]-piperidine-3-carboxylic acid diethylamide. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-(3,5-dimethyl-piperidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-(2-methyl-piperidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-(2-methoxymethyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-((S)-2-methoxymethyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is 1-[8-amino-2-(5-bromo-furan-2-yl)-[1,2,4-]triazolo[1,5-a]pyridine-6-carbonyl]-pyrrolidine-2-carboxylic acid (S)-dimethyl amide. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridine-6-carboxylic acid dimethylcarbamoylmethyl-methyl-amide. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]- -(3-methyl-piperidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridine-6-carboxylic acid methyl-propyl-amide. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridine-6-carboxylic acid ethyl-(2-pyridin-2-yl-ethyl)-amide.
In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-thiomorpholin-4-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-(5-bromo-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyrdine-6-carboxylic acid methyl-phenethyl-amide.
In another embodiment, the adenosine A2a pathway antagonist is (8-Amino-2-furan-2-yl-[1,2,4]triazolo[1,5-a]pyridin-6-yl)-pyrrolidi-n-1-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is (8-amino-2-furan-2-yl-[1,2,4]triazolo[1,5-a]pyridin-6-yl)-(2-R-methoxymethyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is (8-amino-2-furan-2-yl-[1,2,4]triazolo[1,5-a]pyridin-6-yl)-(2-S-methoxymethyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a]pyridine-6-carboxylic acid dibenzylamide.
In another embodiment, the adenosine A2a pathway antagonist is 8-Amino-2-thiophen-2-yl-[1,2,4]triazolo[1,5-a]-pyridine-6-carboxylic acid dibenzyl amide.
In another embodiment, the adenosine A2a pathway antagonist is [8-Amino-2-(5-methyl-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-pyrrolidin-1-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-methyl-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-piperidin-1-yl-methanone. In another embodiment, the adenosine A2a pathway antagonist is [8-amino-2-(5-methyl-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridin-6-yl]-(2-methyl-pyrrolidin-1-yl)-methanone. In another embodiment, the adenosine A2a pathway antagonist is 8-amino-2-(5-methyl-furan-2-yl)-[1,2,4]triazolo[1,5-a]pyridine-6-carboxylic acid methyl-propylamide.
In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:




In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist, has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist has one of the formulas:







In another embodiment, the adenosine A2a pathway antagonist is a pharmaceutically acceptable salt of one of the above adenosine A2a pathway antagonists. In another embodiment, the adenosine A2a pathway antagonist is a related compound with adenosine A2a pathway antagonist activity
In another embodiment, the adenosine A2a pathway antagonist an antagonist adenosine A2a receptor.
In another embodiment, the adenosine A2a pathway antagonist is any other A2a pathway antagonist known in the art. Each adenosine A2a pathway antagonist represents a separate embodiment of the present invention. Each substituent in each position indicated above as having different substituents represents a separate embodiment of the present invention.
In another embodiment, the adenosine A2a receptor that is antagonized is an ADORA2 receptor. In another embodiment, the adenosine A2a receptor is an RDC8 receptor. In another embodiment, the adenosine A2a receptor is an hA2aR receptor.
In another embodiment, the sequence of the target adenosine A2a receptor is:
MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVG VLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAIC WVLSFAIGLTPMLGWNNCGQPICEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLL LMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFF CPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRICIIRSHVLRQQEPFICAAGTSARVLA AHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHE LKGVCPEPPGLDDPLAQDGAGVS (SEQ ID No: 270). In another embodiment, the adenosine A2a receptor is a homologue of SEQ ID No: 270. In another embodiment, the adenosine A2a receptor is a variant of SEQ ID No: 270. In another embodiment, the adenosine A2a receptor is an isomer of SEQ ID No: 270. In another embodiment, the adenosine A2a receptor is a fragment of SEQ ID No: 270. In another embodiment, the adenosine A2a receptor comprises SEQ ID No: 270. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the sequence of the target adenosine A2a receptor is:
MLLETQDALYVALELVIAALSVAGNVLVCAAVGTANTLQTPTNYFLVSLAAADVA VGLFAIPFAITISLGFCTDFYGCLFLACFVLVLTQSSIFSLLAVAVDRYLAICVPLRYKSLVTGTRAR GVIAVLWVLAFGIGLTPFLGWNSKDSATNNCTEPWDGTTNESCCLVKCLFENVVPMSYMVYFNF FGCVLPPLLEVILVIYIKIFLVACRQLQRTELMDHSRTTLQREIHAAKSLAMIVGIFALCWLPVHAVN CVTLFQPAQGKNKPKWAMNMAILLSHANSVVNPIVYAYRNRDFRYTFHKIISRYLLCQADVKSG NGQAGVQPALGVGL (SEQ ID No: 271). In another embodiment, the adenosine A2a receptor is a homologue of SEQ ID No: 271. In another embodiment, the adenosine A2a receptor is a variant of SEQ ID No: 271. In another embodiment, the adenosine A2a receptor is an isomer of SEQ ID No: 271. In another embodiment, the adenosine A2a receptor is a fragment of SEQ ID No: 271. In another embodiment, the adenosine A2a receptor comprises SEQ ID No: 271. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the adenosine A2a receptor has an AA sequence set forth in one of the following GenBank entries: BC025722, M97759, and BC013780. In another embodiment, the adenosine A2a receptor has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the adenosine A2a receptor is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the adenosine A2a receptor is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the adenosine A2a receptor is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the adenosine A2a receptor is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the adenosine A2a receptor comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a DP receptor antagonist is utilized in methods and compositions of the present invention. In another embodiment, a DP-1 receptor antagonist is utilized. In another embodiment, the DP-1 receptor antagonist is one that is used to treat allergic rhinitis. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the DP receptor antagonist utilized in methods and compositions of the present invention has the structure:




wherein R is selected from F, H, isopropyl, Br, CHO, CH2OH, CON(CH3)2, CONH-phenol, SCH3, SOCH3, SO-ethanol, SO-isopropanol, SO-phenol, SO-boron nitride, and SO2CH3.
In another embodiment, the DP receptor antagonist has the structure:




    
    
        wherein R1 is selected from H, SO2CH3, and SO2NCH3.
        and R2 is selected from F, H, isopropyl, Br, CHO, CH2OH, CON(CH3)2, CONH-phenol, SCH3, SOCH3, SO-ethanol, SO-isopropanol, SO-phenol, SO-boron nitride, CH═CH2, Cy (cyclohexyl), CN, 2-thiophene, COCH2, CH(OH)CH3, and SO2CH3.
    
    


In another embodiment, the DP receptor antagonist has the structure:




In another embodiment, the DP receptor antagonist has one of the structures:




In another embodiment, the DP receptor antagonist is 2-[(1R)-9-(4-chlorobenzyl)-8-((R)-methyl-sulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the DP receptor antagonist is 2-[(1R)-9-(4-chlorobenzyl)-8-((S)-methyl-sulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the DP receptor antagonist is a mixture of 2-[(1R)-9-(4-chlorobenzy))-8-((R)-methyl-sulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid and 2-[(1R)-9-(4-chlorobenzyl)-8-((S)-methyl-sulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid.
In another embodiment, the synthetic route for preparing one of the above DP receptor antagonist comprises the steps in Scheme 1:




In another embodiment, the synthetic route for preparing one of the above DP receptor antagonist comprises the steps in Scheme 2:




In another embodiment, the DP receptor antagonist is a variant of the previous structure, wherein the upper right cyclopentyl group is substituted for by a cyclohexyl group. Each of the substituents enumerated for the previous structure can be used for this cyclohexyl-containing structure, and each represents a separate embodiment of the present invention.
In another embodiment, the DP receptor antagonist has one of the following structures:







In another embodiment, the DP receptor antagonist has one of the following structures:




In another embodiment, the DP receptor antagonist is a stereoisomer of 1 of the above structures. In another embodiment, the DP receptor antagonist has an R configuration at the acetic acid side chain. In another embodiment, the DP receptor antagonist is any other stereoisomer of 1 of the above structures. In another embodiment, the DP receptor antagonist is or a compound related to 1 of the above structures, that has DP receptor antagonist activity.
Preparation of the above compounds involves, in another embodiment, a Fischer-indole reaction as the key step in their synthesis (Scheme 1 below). In another embodiment, arylhydrazines (37) are treated with ethyl 2-oxocyclohexanecarboxylate (38) in acetic acid to furnish the corresponding indoles (39). In another embodiment, standard benzylation and ester hydrolysis provides the target compounds (41).




In another embodiment (e.g. in the case of a tetrahydrocyclopenta[b]indole series, the indole core is constructed via a palladium mediated reaction between an ortho-iodoaniline and a cyclopentanone as illustrated by the synthesis of 27 shown in Scheme 2. In another embodiment, after iodination of aniline 42, condensation with ethyl 2-oxocyclopentane-carboxylate followed by treatment with Pd(OAc)2 in DMF yields the desired indole 44. In another embodiment, selective bromination at the 7-position is performed through the actions of pyridinium tribromide to provide 45. In another embodiment, following standard benzylation, a Stille coupling of 46 with 1-(ethoxyvinyl)tributylstannane followed by acidic work-up generates ketone 47. In another embodiment, ester hydrolysis delivers the DP receptor antagonist 27.
Scheme 2. Synthesis of tetrahydrocyclopenta[b]indole series. Reagents and conditions: (a) 12, EtOH, AgSO4; (b) ethyl 2-oxocyclopentanecarboxylate, Pd(OAc)2 DMF; (c) pyrHBr—Br2, pyr, Zn, AcOH; (d) NaH, DMF, 0° C.; 4-ClC6H4CH2Br; (e) 1-1-ethoxyvinylstannane, Pd2(dba)3, Ph3As, DMF, 100° C.; 2-aq HCl; (f) NaOH, H2O, MeOH, THF.




In another embodiment, the DP receptor antagonist has the following structure:




wherein n is 0 or 1; m is 1, 2 or 3; R.sub.1 is H, C.sub.1-C.sub.3 alkyl, halogenated C.sub.1-C.sub.3 alkyl or cyclopropyl; R.sub.2 is 4-chlorophenyl or 2,4,6-trichiorophenyl.

In another embodiment, the above DP receptor antagonist has the following stereochemistry:




In another embodiment, the above DP receptor antagonist is [4-(4-chlorobenzyl)-7-fluoro-5-(methanesulfon-yl)-1,2,3,4-tetrahydrocyclopenta[b]-indol-3-yl]acetic acid. In another embodiment, the above DP receptor antagonist is {4-[1-(4-chlorophenyl)ethyl]-7-fluoro-5-methanesulfonyl-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl}acetic acid. In another embodiment, the above DP receptor antagonist is [9-(4-chlorobenzyl)-6-fluoro-8-methanesulfonyl-2,3,4,9-tetrahydro-1H-carbazol-1-yl]-acetic acid. In another embodiment, the above DP receptor antagonist is [4-(4-chlorobenzyl)-7-fluoro-5-methanesulfonyl-1-oxo-1,2,3,4-tetrahydrocyclopenta-[b]indol-3-yl]acetic acid.
In another embodiment, the above DP receptor antagonist is (3R)-[4-(4-chlorobenzyl)-7-fluoro-5-(methane-sulfonyl)-1,-2,3,4-tetrahydrocyclopenta[b]indol-3-yl]acetic acid. In another embodiment, the above DP receptor antagonist is (.+−.)-4-[1-(4-chlorophenyl)ethyl]-7-fluoro-5-methanesulf-onyl-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl acetic acid. In another embodiment, the above DP receptor antagonist is (.+−.)-[9-(4-chlorobenzyl)-6-fluoro-8-methane-sulfonyl-2,-3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid and pharmaceutically acceptable salts thereof. In another embodiment, the above DP receptor antagonist is [4-(4-chlorobenzyl)-7-fluoro-5-methane-sulfonyl-1-oxo-1,2-,3,4-tetrahydro-cyclopenta[b]indol-3-yl]acetic acid.
In another embodiment, the DP receptor antagonist has the following structure:




    wherein:
    R.sup.1, R.sup.2 and R.sup.3 are each independently selected from the group consisting of:
    
        (1) hydrogen, and (2) R.sup.c,
    
    
    R.sup.4 and R.sup.5 are each independently selected from the group consisting of:
    
        (1) H, (2) F, (3) CN, (4) C.sub.1-6 alkyl, (5) OR.sup.a, and (6) S(O).sub.n C.sub.1-6 alkyl, wherein each of said alkyl group is optionally substituted with halogen, or
    
    
    R.sup.4 and R.sup.5 on the same carbon atom may represent an oxo, or
    R.sup.4 and R.sup.5 on the same carbon atom or on adjacent carbon atoms taken together form a 3- or 4-membered ring containing 0 or 1 heteroatom selected from N, S, or O optionally substituted with one or two groups selected from F, CF.sub.3 and CH.sub.3;


R.sup.6 is selected from the group consisting of:

    
    
        (1) H, (2) C.sub.1-6 alkyl optionally substituted with one to six groups independently selected from OR.sup.a and halogen, and (3) heterocyclyl optionally substituted with one to four halogen; or
    
    
    R.sup.5 and R.sup.6 attached on adjacent carbon atoms together form a 3- or 4-membered ring containing 0 or 1 heteroatom selected from N, S, or O optionally substituted with one or two groups selected from F, CF.sub.3 and CH.sub.3;
    X is selected from the group consisting of: C.dbd.0, SO.sub.2, and C.sub.1-4 alkyl wherein said alkyl is optionally substituted with one to six halogen;


Ar is phenyl each optionally substituted with one to four groups independently selected from R.sup.c;

    Q is C.sub.1-6 alkyl substituted with COOH or tetrazolyl, or
    Q and R.sup.6 together form a 3- or 4-membered ring optionally containing a heteroatom selected from N, S, and O, and optionally substituted with one or two groups independently selected from:
    
        (1) halogen, (2) oxo, (3) OR.sup.a, (4) COOH, (5) C(O)NHSO.sub.2 R.sup.7, and (6) tetrazolyl,
    
    
    R.sup.7 is selected from the group consisting of:
    
        (1) C.sub.1-6 alkyl optionally substituted with one to six halogen, (2) aryl, and (3) heteroaryl,
    
    
    wherein said aryl and heteroaryl are optionally substituted with halogen, OC.sub.1-5 alkyl, C.sub.1-5 alkyl and wherein said alkyl is optionally substituted with one to six halogen;


R.sup.a and R.sup.b are independently selected from hydrogen and C.sub.1-6 alkyl optionally substituted with one to six halogen;

    R.sup.c is
    
        (1) halogen, (2) CN, (3) C.sub.1-6 alkyl optionally substituted with one to six groups independently selected from halogen, NR.sup.a R.sup.b, C(O)R.sup.a, C(OR.sup.a)R.sup.a R.sup.b, and OR.sup.a, (4) C.sub.2-6 alkenyl optionally substituted with one to six groups independently selected from halogen and OR.sup.a, (5) heterocyclyl, (6) aryl, (7) heteroaryl, (8) C(O)R.sup.a, (9) C(OR.sup.a)R.sup.a R.sup.b, (10) C(O)OR.sup.a, (11) CONR.sup.a R.sup.b, (12) OCONR.sup.a R.sup.b, (13) S(O).sub.n R.sup.7, (14) NR.sup.a C(O)OC.sub.1-6 alkyl, wherein alkyl is optionally substituted with one to six halogen and (15) S(O).sub.n NR.sup.a R.sup.b,
    
    
    wherein heterocyclyl, aryl, heteroaryl are optionally substituted with one to four groups independently selected from halogen; and
    n is 1, 1 or 2.


In another embodiment, the DP receptor antagonist is MK-0524, MK-0524A, or MK-0524B (all obtainable from Merck), or a related compound with DP receptor antagonist activity.
In another embodiment, the DP receptor antagonist has the following structure:




wherein R is selected from Cl or 1 of the following structures:





In another embodiment, the DP receptor antagonist has the structure:




wherein R is defined as above, and the R group is at a position selected from meta, ortho, and para.

In another embodiment, the DP receptor antagonist has the following structure:




wherein the n-butyloxy group is at a position selected from meta, ortho, and para.

In another embodiment, the DP receptor antagonist has the following structure:




wherein R is defined as above, and R2 is selected from OCH3, H, CH3, isopropyl, F, Cl, and OH.

In another embodiment, the DP receptor antagonist has the following structure:




wherein R is defined as above, and R2 is selected from OCH3, H, CH3, isopropyl, F, Cl, and OH.

In another embodiment, the DP receptor antagonist has the following structure:




wherein R is defined as above, and R2 is selected from:





In another embodiment, the DP receptor antagonist has the following structure:




wherein R is selected from:





In another embodiment, the DP receptor antagonist has the following structure:




wherein R is defined as above, and R2 is selected from:





In another embodiment, the DP receptor antagonist has the following structure:




wherein R is selected from:





In another embodiment, the DP receptor antagonist has the following structure:




    wherein
    R.sup.1, R.sup.2 and R.sup.3 are each independently selected from hydrogen and R.sup.g;
    R.sup.4 is selected from H, CN, C.sub.1-6alkyl optionally substituted with one to six halogen, OR.sup.a and S(O).sub.nC.sub.1-6alkyl;
    R.sup.5 is selected from H and C.sub.1-6alkyl optionally substituted with one to six halogen; or
    R.sup.4 and R.sup.5 together represent an oxo; or
    R.sup.4 and R.sup.5 taken together form a 3- or 4-membered ring containing 0 or 1 heteroatom selected from NR.sup.f, S, and O optionally substituted with one or two groups selected from F, CF.sub.3 and CH.sub.3;
    R.sup.6 is selected from H and C.sub.1-6alkyl optionally substituted with one to six groups independently selected from OR.sup.a and halogen,
    Ar is aryl or heteroaryl each optionally substituted with one to four groups independently selected from R.sup.g;
    A is C.sub.1-3alkyl optionally substituted with one to four halogen atoms, O(CH.sub.2)12, S(CH.sub.2).sub.1-2;
    Q is selected from: (1) COOH, (2) CONR.sup.aR.sup.b, (3) C(O)NHSO.sub.2R.sup.c, (4) SO.sub.2NHR.sup.a, (5) SO.sub.3H, (6) PO.sub.3H.sub.2, and (7) tetrazolyl,
    Y.sup.1 is —(CR.sup.dR.sup.e).sub.a- —X—(CR.sup.dR.sup.e).sub.b--, phenylene, C.sub.3-6cycloalkylidene or C.sub.3-6cycloalkylene, wherein a and b are integers 0-1 such that the sum of a and b equals 0, 1 or 2;
    X is a bond, O, S, NR.sup.a, C(O), OC(O), C(O)O, C(O)NR.sup.a, OC(O)NR.sup.a, NR.sup.aC(O), CR.sup.d.dbd.CR.sup.e or C.ident.C;
    Y.sup.2 is CR.sup.dR.sup.e, CR.sup.dR.sup.e--CR.sup.dR.sup.e, or CR.sup.d.dbd.CR.sup.e,
    R.sup.a and R.sup.b are independently selected from H, C.sub.1-10alkyl, C.sub.2-10alkenyl, C.sub.2-10alkynyl, Cy and Cy C.sub.1-10alkyl, wherein alkyl, alkenyl, alkynyl and Cy are optionally substituted with one to six substituents independently selected from halogen, amino, carboxy, C.sub.1-4alkyl, C.sub.1-4alkoxy, aryl, heteroaryl, aryl C.sub.1-4alkyl, hydroxy, CF.sub.3, OC(O)C.sub.1-4alkyl, OC(O)NR.sup.iR.sup.i, and aryloxy; or
    R.sup.a and R.sup.b together with the atom(s) to which they are attached form a heterocyclic ring of 4 to 7 members containing 0-2 additional heteroatoms independently selected from oxygen, sulfur and N—R.sup.f;
    R.sup.c is selected from C.sub.1-6alkyl optionally substituted with one to six halogen, aryl and heteroaryl, wherein said aryl and heteroaryl are optionally substituted with halogen, OC.sub.1-6alkyl, C.sub.1-6alkyl and wherein said alkyl is optionally substituted with one to six halogen;
    R.sup.d and R.sup.e are independently selected from H, halogen, aryl, heteroaryl, C.sub.1-6alkyl or haloC.sub.1-6alkyl, or
    R.sup.f is selected from H, C.sub.1-6alkyl, haloC.sub.1-6alkyl, Cy, C(O)C.sub.1-6alkyl, C(O)haloC.sub.1-6 alkyl, and C(O)—Cy,
    R.sup.g is selected from (1) halogen, (2) CN, (3) C.sub.1-6alkyl optionally substituted with one to eight groups independently selected from aryl, heteroaryl, halogen, NR.sup.aR.sup.b, C(O)R.sup.a, C(OR.sup.a)R.sup.aR.sup.b, SR.sup.a and OR.sup.a, wherein aryl, heteroaryl and alkyl are each optionally substituted with one to six groups independently selected from halogen, CF.sub.3, and COOH, (4) C.sub.2-6alkenyl optionally substituted with one to six groups independently selected from halogen and OR.sup.a, (5) Cy (6) C(O)R.sup.a, (7) C(O)OR.sup.a, (8) CONR.sup.aR.sup.b, (9) OCONR.sup.aR.sup.b, (10) OC.sub.1-6alkyl, wherein alkyl is optionally substituted with one to six substituents selected from halogen, aryl, heteroaryl, OH and OC(O)R.sup.a, (11) O-aryl (12) O-heteroaryl (13) S(O).sub.nC.sub.1-6alkyl, wherein alkyl is optionally substituted with one to six substituents selected from halogen, aryl, heteroaryl, OH, and OC(O)R.sup.a, (14) S(O).sub.naryl, (15) S(O)nheteroaryl, (16) —NR.sup.aS(O).sub.nR.sup.b, (17) —NR.sup.aR.sup.b, (18) —NR.sup.aC(O)R.sup.b, (19) —NR.sup.aC(O)OR.sup.b, (20) —NR.sup.aC(O)NR.sup.aR.sup.b, (21) S(O).sub.nNR.sup.aR.sup.b, (22) NO.sub.2, (23) C.sub.5-8cycloalkenyl, wherein Cy is optionally substituted with one to eight groups independently selected from halogen, C(O)R.sup.a, OR.sup.a, C.sub.1-3alkyl, aryl, heteroaryl and CF.sub.3;
    R.sup.i and R.sup.j are independently selected from hydrogen, C.sub.1-1 Oalkyl, Cy and Cy-C.sub.1-10alkyl; or
    R.sup.i and R.sup.j together with the carbon to which they are attached form a ring of 5 to 7 members containing 0-2 heteroatoms independently selected from oxygen, sulfur and nitrogen;
    Cy is selected from heterocyclyl, aryl, and heteroaryl; and
    n is 0, 1 or 2.


In another embodiment, a DP receptor antagonist of the formula I above has the structure:




In another embodiment, a DP receptor antagonist of formula I above has the structure:




wherein Y.sup.1 is O, S, C(O) or CH.sub.2, R.sup.4 and R.sup.5 are each hydrogen or R.sup.4 and R.sup.5 together represent oxo, and R.sup.2 and R.sup.3 represent one or two non-H substituents.

In another embodiment, a DP receptor antagonist of formula I above has the structure:




wherein Y.sup.1 is C(O), CH.sub.2 or S, R and R′ are independently hydrogen, halogen, cyano, C.sub.1-3alkanoyl or CF.sub.3, R.sup.2 is selected from halogen, S(O).sub.nC.sub.1-3alkyl, OC.sub.1-6alkyl (optionally substituted with aryl), CN, C.sub.2-6alkenyl, 1- or 2-methyltetrazolyl, 1-methylpyroolyl and C.sub.1-6alkyl, and R.sup.3 is selected from halogen, S(O).sub.nC.sub.1-3alkyl, OC.sub.1-6alkyl C(O)R.sup.a, C.sub.1-6alkyl (optionally substituted with 3 to 6 halogen atoms, and 0 or 1 group select from OR.sup.a, SR.sup.a,), C.sub.2-6alkenyl, C.sub.5-8cycloalkenyl, phenyl (optionally substituted with a group selected from C.sub.1-3alkyl, OR.sup.a and pyrazolyl), naphthyl, and heteroaryl selected from pyrrolyl, thienyl, pyrazolyl, quinolinyl, benzothienyl, isoxalyl, pyridyl, each of which is optionally substituted with C.sub.1-3alkyl.
In another embodiment, the DP receptor antagonist is a compound with DP receptor antagonist activity, related to 1 of the above structures.
Synthesis of the above compounds is carried out, in another embodiment, as depicted in Scheme 1 below. Formation of a hydrazone of 12 with acetaldehyde in toluene, followed by N-acylation of another NH moiety with acid chlorides 13a-j, affords the N-acyl hydrazones 14a-j, respectively. Acidic hydrolysis of 14a-j, followed by conventional indole synthesis using levulinic acid, yields the N-benzoyl-5-methoxy-2-methylindole-3-acetic acids 2a-j, respectively. In another embodiment, as shown in Scheme 2 below, compounds are prepared from their corresponding 4-substituted phenylhydrazines (15a-e, respectively) according to the same procedures described above.












In another embodiment, synthesis of the above compounds is carried out as depicted in Scheme 3. Replacement of the methoxy moiety of 17 with a benzyloxy moiety is achieved as follows: Demethylation of the 5-methoxy group of 17 with pyridinium chloride at 180° C., followed by benzylation of a formed phenol derivative 18 with benzyl bromide, affords a benzyl ether 19, N-acylation of which followed by catalytic hydrogenation yields 4. In another embodiment, compound 5 is prepared from the corresponding indole derivative 21 according to the N-acylation procedure described above.




In another embodiment, synthesis of the above compounds is carried out as depicted in Scheme 4. Compounds are prepared according to the usual indole synthesis procedure using N-acyl hydrazine 22 and the corresponding keto carboxylic acids 24 and 25a-c, respectively. In another embodiment, compounds are prepared by deprotection of the allyl ester 23, which was prepared from 22 and 26 by the indole synthesis procedure described above. In another embodiment, compounds are prepared from 5-methoxy-2-methylindole 27 as outlined in Scheme 5. Treatment of 27 with dimethylformamide in the presence of phosphorus oxychloride yields 28, C2 homologation of which by the Wittig reaction results in 29. Alkaline hydrolysis of 29 provides the corresponding carboxylic acid 30, N-acylation of which with 4-nbutoxybenzoylchloride in the presence of n-BuLi affords 11. In another embodiment, synthesis of 2-methylindole-4-acetic acid 1 is outlined in Scheme 6. Methoxycarbonylation of 32, which is prepared from 31 by the usual method, is carried out by the palladium-catalyzed carbon monoxide insertion reaction in the presence of methanol. Alkaline hydrolysis of 33, followed by O-benzylation, provides 35, after which N-acylation of 35 with an acid chloride 13d and deprotection with hydrogenolysis affords a carboxylic acid 37. Compound 37 is converted to 38 by the conventional C-1 homologation procedure, followed by O-benzylation. Deprotection of 38 with hydrogenolysis-yields 1.












In another embodiment, the DP receptor antagonist has the structure:




    wherein:
    R represents —COR.sup.1, —CH.sub.2OR.sup.0, or —COOR.sup.20, R.sup.0 represents a hydrogen atom or C2-6 acyl group, R.sup.1 represents a hydroxyl group, C1-6 alkoxy group, —NR.sup.8R.sup.9 wherein R.sup.8 and R.sup.9 each represents hydrogen atom, C1-6 alkyl group, —SO.sub.2R.sup.13 wherein R.sup.13 represents C1-6 alkyl group, C3-15 saturated or unsaturated carbocyclic ring, or 4 to 15-membered heterocycle containing 1 to 5 of nitrogen atom(s) sulfur atom(s) and/or oxygen atom(s), R.sup.20 represents allyl or benzyl group;
    R.sup.2 represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, C2-6 alkoxyalkyl, a halogen atom, amino, trihalomethyl, cyano, hydroxyl, benzyl, or 4-methoxybenzyl group;
    R.sup.3 represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, a halogen atom, trihalomethyl, cyano, or hydroxyl group;
    R.sup.4 and R.sup.5 each represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, C2-6 alkoxyalkyl group, a halogen atom, nitro, amino, trihalomethyl, trihalomethoxy, cyano or hydroxyl group, D represents a single bond, C1-6 alkylene, C2-6 alkenylene, or C1-6 oxyalkylene group;
    --G-R.sup.6 represents:
    
        1) G represents C1-6 alkylene that may be replaced by 1 or 2 of oxygen atom(s) and/or sulfur atom(s), C2-6 alkenylene that may be replaced by 1 or 2 of oxygen atom(s) and/or sulfur atom(s), wherein the alkylene and alkenylene groups may be substituted by hydroxyl or C1-4 alkoxy group, —C(O)NH—, —NHC(O)—, —SO.sub.2NH—, —NHSO.sub.2--, or diazo group,
        R.sup.6 represents C3-15 saturated or unsaturated carbocyclic ring, or a 4 to 15-membered heterocycle containing 1 to 5 of nitrogen atom(s) sulfur atom(s), and/or oxygen atom(s), wherein the rings may be substituted by 1 to 5 group(s) selected from C1-6 alkyl, C1-10 alkoxy, C2-6 alkoxyalkyl, a halogen atom, hydroxyl, trihalomethyl, nitro, amino, phenyl, phenoxy, oxo, C2-6 acyl, C1-6 alkane sulphonyl, and cyano group,
    
    
    or
    
        2) together with G and R.sup.6,
        (i) C1-15 alkyl group that may be replaced by 1 to 5 of oxygen atom(s) and/or sulfur atom(s),
        (ii) C2-15 alkenyl group that may be replaced by 1 to 5 of oxygen atom(s) and/or sulfur atom(s), wherein the alkyl, alkenyl, and alkynyl groups may be substituted by 1 to 12 of groups selected from C1-6 alkoxy, a halogen atom, hydroxyl, cyano, oxo groups, and —NR.sup.11R.sup.12 wherein R.sup.11 and R.sup.12 represent hydrogen atoms, C1-6 alkyl, C2-6 alkenyl, phenyl, benzoyl, naphthyl, phenyl groups substituted with C1-6 alkyl group, or C1-6 alkyl group substituted by phenyl or cyano group, respectively,
    
    
    n represents 1 to 3,
    m represents 1 to 3,
    i represents 1 to 4,
    
        
    
    
    represents a single bond or a double bond,


In another embodiment of the above structure, the 4 to 15-membered heterocycle that contains 1 to 5 of nitrogen, sulfur, and oxygen atom(s) in this specification is saturated. In another embodiment, the 4 to 15-membered heterocycle is unsaturated. In another embodiment, the 4 to 15-membered heterocycle is selected from the following formulas:







In other embodiments, the DP receptor antagonist is a non-toxic salt of one of the above compounds.
In other embodiments, the DP receptor antagonist is selected from the following compounds: (1) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid benzyl ester, (2) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (3) 1-(4-((2R)-1,4-benzodioxan-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (4) 1-(4-((2S)-1,4-benzodioxan-2-ylmethoxy)benzoyl)-2-methyl-1H-indole-4-yl acetic acid, (5) 1-(4-((2S)-1-methylindolin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (6) 1-(4-((2R)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (7) 1-(4-((2S)-4,6-dimethyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (8) 1-(4-((2S)-6-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (9) 1-(4-((2S)-6-methoxy-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (10) 1-(4-((2S)-7-methoxy-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (11) 1-(4-((2S)-4,7-dimethyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (12) 1-(4-((2S)-7-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (13) 1-(4-((2S)-1-ethylindolin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (14) 1-(4-((2S)-4,8-dimethyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (15) 1-(4-((2S)-5-methoxy-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (16) 1-(4-((2S)-5-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (17) 1-(4-((2S)-2,3-dihydrobenzofuran-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (18) 1-(4-((2R)-2,3-dihydrobenzofuran-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (19) 1-(4-((2S)-8-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (20) 1-(4-((2R)-1,4-benzoxathian-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (21) 1-(4-((3S)-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (22) 1-(4-((3S)-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (23) 1-(4-((3R)-5-fluoro-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (24) 1-(4-((3R)-5-methyl-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (25) 1-(4-((3R)-6-fluoro-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (26) 1-(4-((3R)-7-methyl-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (27) 1-(4-((2R)-5-fluoro-1,4-benzodioxan-2-yl methoxy)benzoyl)-2-methyl-1H ndol-4-yl acetic acid, (28) 1-(4-((2S)-8-fluoro-1,4-benzodioxan-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (29) 1-(4-((3R)-7-fluoro-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl acetic acid, (30) 3-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)acrylic acid allyl ester, (31) 3-(1-(4-((2S)-4-methyl-3,4-di hydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)acrylic acid, (32) 3-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)propanoic acid, (33) (1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)oxyacetic acid benzyl ester, (34) (1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)oxyacetic acid, (35) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-carboxylic acid benzyl ester, (36) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-carboxylic acid, (37) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-1H-indol-4-yl acetic acid benzyl ester, (38) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-1H-indol-4-yl acetic acid, (39) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2,3-dihydro-1H-indol-4-yl acetic acid, (40) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-2,3-dihydro-1H-indol-4-yl acetic acid, (41) 2-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethanol, (42) 2-(1-(4-((2S)-4,6-dimethyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethanol, (43) 2-(1-(4-((2S)-6-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-ypethanol, (44) 2-(1-(4-((2S)-7-fluoro-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethanol, (45) 2-(1-(4-((2S)-2,3-dihydrobenzofuran-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethanol, (46) 2-(1-(4-((3R)-2,3-dihydrobenzofuran-3-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethanol, (47) N-methylsulfonyl-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)acetamide, (48) N-phenylsulfonyl-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)acetamide, (49) 2-(1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)benzoyl)-2-methyl-1H-indol-4-yl)ethyl acetate, (50) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)-2-methylbenzoyl)-2-methyl-1H-indol-4-yl acetic acid benzyl ester, (51) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)-2-methylbenzoyl)-1H-indol-4-yl acetic acid benzyl ester, (52) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)-2-methylbenzoyl)-2-methyl-1H-indol-4-yl acetic acid, and (53) 1-(4-((2S)-4-methyl-3,4-dihydro-2H-1,4-benzoxazin-2-ylmethoxy)-2-methylbenzoyl)-1H-indol-4-yl acetic acid or a non-toxic salt thereof.
In other embodiments, the DP receptor antagonist has the structure:




wherein

R.sup.1 represents hydroxy, C1-6 alkoxy, or NR.sup.8R.sup.9, in which R.sup.8 and R.sup.9 each independently represents a hydrogen atom, C1-6 alkyl, or SO.sub.2R.sup.13, in which R.sup.13 represents C1-6 alkyl, a C3-15 saturated or unsaturated carbocyclic ring or a 4- to 15-membered heterocyclic ring containing 1 to 5 nitrogen atom(s), sulfur atom(s) and/or oxygen atom(s);

R.sup.2 represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, C2-6 alkoxyalkyl, a halogen atom, amino, trihalomethyl, cyano, hydroxy, benzyl, or 4-methoxybenzyl;

R.sup.3 represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, a halogen atom, trihalomethyl, cyano, or hydroxy;

R.sup.4 and R.sup.5 each independently represents a hydrogen atom, C1-6 alkyl, C1-6 alkoxy, C2-6 alkoxyalkyl, a halogen atom, nitro, amino, trihalomethyl, cyano, or hydroxy;

D represents a single bond, C1-6 alkylene, C2-6 alkenylene, or C1-6 oxyalkylene;

in --G--R.sup.6,

1)

    
    
        G represents a single bond, C1-6 alkylene which may be substituted with 1 to 2 oxygen atom(s) and/or sulfur atom(s), C2-6 alkenylene which may be substituted with 1 to 2 oxygen atom(s) and/or sulfur atom(s), in which the alkylene and the alkenylene may be substituted with hydroxy or C1-4 alkoxy, —C(O)NH—, —NHC(O)—, —SO.sub.2NH—, —NHSO.sub.2--, or diazo;
        R.sup.6 represents a C3-15 saturated or unsaturated carbocyclic ring, or a 4- to 15-membered heterocyclic ring containing 1 to 5 nitrogen atom(s), sulfur atom(s) and/or oxygen atom(s), in which the ring may be substituted with 1 to 5 substituent(s) selected from C1-6 alkyl, C1-10 alkoxy, C2-6 alkoxyalkyl, a halogen atom, hydroxy, trihalomethyl, nitro, amino, phenyl, phenoxy, oxo, C2-6 acyl, C1-6 alkanesulfonyl and cyano,

2) G and R.sup.6 are taken together to represent

        (i) C1-15 alkyl which may be substituted with 1 to 5 oxygen atom(s) and/or sulfur atom(s);
        (ii) C2-15 alkenyl which may be substituted with 1 to 5 oxygen atom(s) and/or sulfur atom(s); or
        (iii) C2-15 alkynyl which may be substituted with 1 to 5 oxygen atom(s) and/or sulfur atom(s),
        in which the alkyl, the alkenyl and the alkynyl may be substituted with 1 to 12 substituent(s) selected from C1-6 alkoxy, a halogen atom, hydroxy, cyano, oxo and NR.sup.11R.sup.12, in which R.sup.11 and R.sup.12 each independently represents a hydrogen atom, C1-6 alkyl, C2-6 alkenyl, phenyl, benzoyl, naphthyl, phenyl substituted with C1-6 alkyl, or C1-6 alkyl substituted with phenyl or cyano;

n represents 1 to 3;

m represents 1 to 3;

i represents 1 to 4; and

        

represents a single bond or a double bond

    
    


In another embodiment, the DP receptor antagonist has one of the following structures:







In another embodiment, the DP receptor antagonist has one of the following structures:







In another embodiment, the DP receptor antagonist has one of the following structures:







In another embodiment, the DP receptor antagonist has one of the following structures:




In another embodiment, the DP receptor antagonist has the following structure:




In another embodiment, the DP receptor antagonist is [5-[(4-Chlorophenyl)thio]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(4-Chlorophenyl)thio]-4-(methylthio)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(3,4-Dichlorophenyl)thio]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-(4-Chlorobenzoyl)-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-(4-Bromophenyl)thio]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [9-[(3,4-Dichlorophenyl)thio]-1-(methylsulfonyl)-7,8-dihydro-6H-1-pyrido[3,4-b]pyrrolizin-8-yl]acetic acid. In another embodiment, the DP receptor antagonist is [10-[(3,4-Dichlorophenyl)sulfanyl]-1-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,4-b]indolizin-9-yl]acetic acid. In another embodiment, the DP receptor antagonist is (4-(Methylsulfonyl)-5-{[4-(trifluoromethyl)phenyl]thio}-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl)acetic acid. In another embodiment, the DP receptor antagonist is [5-[(2-Chloro-4-fluorophenyl)thiol]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [4-(Methylsulfonyl)-5-(2-naphthylthio)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(2,3-Dichlorophenyl)thio]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(4-Methylphenyl)thiol]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [4-(Methylsulfonyl)-5-(phenylthio)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(2,4-Dichlorophenyl)thiol-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[3,2-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [5-[(4-Chlorophenyl)thio]-4-(methylsulfonyl)-6,7,8,9-tetrahydropyrido[4,3-b]indolizin-6-yl]acetic acid. In another embodiment, the DP receptor antagonist is [9-[(4-Chlorophenyl)thio]-1-(methylsulfonyl)-7,8-dihydro-6H-pyrido[3,4-b]pyrrolizin-8-yl]acetic acid. In another embodiment, the DP receptor antagonist is (−)-[(4-Chlorobenzyl)-7-fluoro-5-methanesulfonyl)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl]acetic acid. In another embodiment, the DP receptor antagonist is (±)-}4-[1-(4-Chlorophenyl)ethyl]-7-fluoro-5-methanesulfonyl-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl}acetic acid. In another embodiment, the DP receptor antagonist is (±)-[9-(4-Chlorobenzyl)-6-fluoro-methanesulfonyl-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the DP receptor antagonist is [4-(4-Chlorobenzyl)-7-fluoro-5-methanesulfonyl-1-oxo-1,2,3,4-tetrahydrocyclopenta indol-3-yl]acetic acid. In another embodiment, the DP receptor antagonist is {9-[(3,4-Dichlorophenyl)thio]-1-isopropyl-7,8-dihydro-6H-pyrido[3,4-b]pyrrolizin-8-yl}acetic acid, Enantiomer A. In another embodiment, the DP receptor antagonist is {9-[(3,4-Dichlorophenyl)thio]-1-isopropyl-7,8-dihydro-6H-pyrido[3,4-b]pyrrolizin-8-yl}acetic acid, Enantiomer B. In another embodiment, the DP receptor antagonist is ((1R)-6-Fluoro-8-(methylsulfonyl)-9-{(1S)-1-[4-(trifluoromethyl)phenyl]ethyl}-2,3,4,9-tetrahydro-1H-carbazol-1-yl)acetic acid. Each possibility represents a separate embodiment of the present invention.
In other embodiments, the DP receptor antagonist is a non-toxic salt of one of the above compounds.
Methods for synthesizing the above DP receptor antagonists are well known in the art, and are described, for example, in the following patent applications: WO03/062200, published on Jul. 31, 2003; WO01/66520, published on Sep. 13, 2001; WO03/022814, published on Mar. 20, 2003; and WO03/078409, published on Sep. 25, 2003.
Each method of synthesis of a DP receptor antagonist represents a separate embodiment of the present invention.
In another embodiment, the DP receptor antagonist is a pharmaceutically acceptable salt of one of the above DP receptor antagonists. In another embodiment, the DP receptor antagonist is a related compound with DP receptor antagonist activity.
In another embodiment, the DP receptor antagonist is any other DP receptor antagonist known in the art
Each DP receptor antagonist and DP-1 antagonist represents a separate embodiment of the present invention. Each substituent in each moiety indicated above as having different possible substituents represents a separate embodiment of the present invention. Each position in each moiety indicated above as having different possible positions represents a separate embodiment of the present invention.
The DP receptor that is inhibited by methods and compositions of the present invention has, in another embodiment, the sequence:
MKSPFYRCQNTTSVEKGNSAVMGGVLFSTGLLGNLLALGLLARSGLGWCSR RPLRPLPSVFYMLVCGLTVTDLLGKCLLSPVVLAAYAQNRSLRVLAPALDNSLCQAFAFFMS FFGLSSTLQLLAMALECWLSLGHPFFYRRHITLRLGALVAPVVSAFSLAFCALPFMGFGKFV QYCPGTWCFIQMVHEEGSLSVLGYSVLYSSLMALLVLATVLCNLGAMRNLYAMHRRLQRH PRSCTRDCAEPRADGREASPQPLEELDHLLLLALMTVLFTMCSLPVIYRAYYGAFKDVKEKN RTSEEAEDLRALRFLSVISIVDPWIFIIFRSPVFRIFFHKIFIRPLRYRSRCSNSTNMESSL (SEQ ID No: 268). In another embodiment, the DP receptor is a homologue of SEQ ID No: 268. In another embodiment, the DP receptor is a variant of SEQ ID No: 268. In another embodiment, the DP receptor is an isomer of SEQ ID No: 268. In another embodiment, the DP receptor is a fragment of SEQ ID No: 268. In another embodiment, the DP receptor comprises SEQ ID No: 268. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the DP receptor is encoded by a nucleotide sequence having the sequence:
cgcccgagccgcgcgcggagctgccgggggctccttagcacccgggcgccggggccctcgcccttccgcagccttcac tccagccctctgctcccgcacgccatgaagtcgccgttctaccgctgccagaacaccacctctgtggaaaaaggcaactcggcggtgatgggcg gggtgctcttcagcaccggcctcctgggcaacctgctggccctggggctgctggcgcgctcggggctggggtggtgctcgcggcgtccactgcg cccgctgccctcggtcttctacatgctggtgtgtggcctgacggtcaccgacttgctgggcaagtgcctcctaagcccggtggtgctggctgcctac gctcagaaccggagtctgcgggtgcttgcgcccgcattggacaactcgagtgccaagccttcgccttcticatgtccactagggctctcctcgaca ctgcaactcctggccatggcactggagtgctggctaccctagggcacccatatctaccgacggcacatcaccctgcgcctgggcgcactggtg gccccggtggtgagcgccttctccctggctttctgcgcgctaccutcatgggcttcgggaagttcgtgcagtactgccccggcacctggtgctttat ccagatggtccacgaggagggctcgctgtcggtgctggggtactctgtgctctactccagcctcatggcgctgctggtcctcgccaccgtgctgtg caacctcggcgccatgcgcaacctctatgcgatgcaccggcggctgcagcggcacccgcgctcctgcaccagggactgtgccgagccgcgcg cggacgggagggaagcgtcccctcagcccctggaggagctggatcacctcctgctgctggcgctgatgaccgtgctcttcactatgtguctctgc ccgtaatttatcgcgcttactatggagcatttaaggatgtcaaggagaaaaacaggacctctgaagaagcagaagacctccgagccttgcgatttct atctgtgatttcaattgtggacccttggatttttatcattttcagatctccagtatttcggatatttttcacaagattttcattagacctcttaggtacaggagc cggtgcagcaattccactaacatggaatccagtctgtgacagtgtttttcactctgtggtaagctgaggaatatgtcacattttcagtcaaagaaccatg attaaaaaaaaaaagacaacttacaatttaaatccttaaaagttacctcccataacaaaagcatgtatatgtattttcaaaagtatttgatatcttaacaat gtgttaccattctatagtcatgaacccatcagtgcattttcattttittattaacagcaactaaaattttatatattgtaaccagtgttaaaagtcttaaaaaa caatggtattaattgtccctacatttgtgcttggtggccctattttatttatagagaggccttgagacatacaggtatttaaaatacagtagaaacacca ctgtttacgattatacgatggacattcataaaaagcataatttcttaccctattcattttttggtgaaacctgattcattgattttatatcattgccgatgtttag ttcatttattgccaattgatctaagcatagcctgaattatgatgttcctcagagaagtgaggtgggaaatatgaccaggtcaggcagttggaggggct tccccagccaccatcggggagtacttgctgcctcaggtggagacctgaagctgtaactagatgcagagcaagatatgactatagcccacaaccca aagaagcaaaaattcgtttttatcttttgaaatccagtttcttttgtattgagtcaagggtgtcagtaggaatcaaaagttgggggtgggttgcaaaatgtt ctttcagtttttagaacctccattttataaaagaattatcctatcaatggattctttagtggaaggatttatgcttctttgaaaaccagtgtgtgactcactgta gagccatgtttactgtttgactgtgtggcacaggggggcatttggcacagcaaaaagcccacccaggacttagcctcagttgacgatagtaacaat ggccttaacatctaccttaacagctacctattacagccgtanctgctgtccgtggagacggtaagatcttaggttccaagattttacttcaaattacacc ttcaaaactggagcagcatatagccgaaaaggagcacaactgagcactttaatagtaatttaaaagttttcaagggtcagcaatatgatgactgaaag ggaaaagtggaggaaacgcagctgcaactgaagcggagactctaaacccagcttgcaggtaagagctttcacctttggtaaaagaacagctggg gaggttcaaggggtttcagcatctctggagttcctttgtatctgacaatctcaggactccaaggtgcaaagcctgctgcatttgcgtgatctcaagacc tccagccagaagtccatccaaatataagagtactcatgtttatttatttccaactgagcagcaacctcctttgatcacttatgtutttccagtatctgaga taatataaagctgggtaattttttatgtaattttttggtatagcaaaactgtgaaaaagccaaattaggcatacaaggagtatgatttaacagtatgacatg atgaaaaaaatacagttgtttttgaaatttaacttttgtttgtaccttcaatgtgtaagtacatgcatgttttattgtcagaggaagaacatgttttttgtattctt tttttggagaggtgtgttaggataattgtccagttaatttgaaaaggccccagatgaatcaataaatataattttatagtaaaaaaaaaaaaaaaaaaaa aaaaa. (SEQ ID No: 269). In another embodiment, the DP receptor is encoded by a nucleotide molecule that a homologue of SEQ ID No: 269. In another embodiment, the nucleotide molecule is a variant of SEQ ID No: 269. In another embodiment, the nucleotide molecule is an isomer of SEQ ID No: 269. In another embodiment, the nucleotide molecule is a fragment of SEQ ID No: 269. In another embodiment, the nucleotide molecule comprises SEQ ID No: 269. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the DP receptor has an AA sequence set forth in one of the following GenBank entries: BC040968, AK026202, and U31099. In another embodiment, the DP receptor has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the DP receptor is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the DP receptor is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the DP receptor is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the DP receptor is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the DP receptor comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a DP-2 receptor antagonist is utilized in methods and compositions of the present invention. In another embodiment, the antagonist is any DP-2 antagonist known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of the present invention comprises the use of a prostaglandin antagonist. In another embodiment, the prostaglandin antagonist has the formula:




wherein:

R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 are each independently selected from:

    
    
        (1) hydrogen; (2) alkyl having 1 to 6 carbon atoms; (3) alkenyl having 2 to 6 carbon atoms;
        (4) —(CH.sub.2).sub.n M, wherein n is 0 to 3 and M is
        
            (a) OR.sup.13; (b) halogen; (c) CF.sub.3; (d) SR.sup.13; (e) phenyl or substituted phenyl, wherein substituted phenyl is as defined below in the definition of R.sup.13; (f) COOR.sup.14; (g)
        
        
    
    






    
    
        
        
            (h) tetrazole; (i)
        
        
    
    






    
    
        
        
            wherein R.sup.16 is C.sub.1 to C.sub.6 alkyl, benzyl or phenyl; (j) —NR.sup.14 R.sup.14; (k) —NHSO.sub.2 R.sup.17, wherein R.sup.17 is C.sub.1 to C.sub.6 alkyl, 4-methylphenyl, phenyl, or CF.sub.3; (1)
        
        
    
    






    
    
        
        
            (m) —SOR.sup.13; (n) —CONR.sup.14 R.sup.14; (o) —SO.sub.2 NR.sup.14 R.sup.14; (p) —SO.sub.2 R.sup.13; (q) NO.sub.2; (r)
        
        
    
    






    
    
        
        
            (v) N.sub.3; and (u) CN;

R.sup.7 is H or alkyl of 1 to 6 carbons;

R.sup.8 is H or alkyl of 1 to 6 carbon atoms;

each R.sup.9 is independently H, OH, C.sub.1 to C.sub.4-O-alkyl or alkyl of 1 to 4 carbons;

R.sup.10 is COOH; CH.sub.2 OH; CHO; tetrazole; NHSO.sub.2 R.sup.11 wherein R.sup.11 is OH, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl or alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl to 1 to 6 carbons; CONHSO.sub.2 R.sup.11, hydroxymethylketone; CN; or CON(R.sup.9).sub.2;

r is 1 to 6;

each R.sup.13 independently is H; C.sub.1 to C.sub.6 alkyl; benzyl, phenyl or substituted phenyl wherein the substituents are C.sub.1 to C.sub.3 alkyl, halogen, CN, CF.sub.3, COOR.sup.14, CH.sub.2 COOR.sup.14, C.sub.1 to C.sub.3 alkoxy, or C.sub.1 to C.sub.4 perfluoroalkyl;

each R.sup.14 is independently H, phenyl, benzyl or C.sub.1 to C.sub.6 alkyl; and

each R.sup.15 independently is H, (CH.sub.2).sub.m COOR.sup.14 wherein m is 0 to 4, C.sub.1 to C.sub.6 alkyl, CF.sub.3, phenyl, or substituted phenyl wherein substituted phenyl is as defined above in the definition of R.sup.13.

        
        
    
    


In another embodiment, the prostaglandin antagonist is a pharmaceutically acceptable salt of one of the above prostaglandin antagonists. In another embodiment, the prostaglandin antagonist is a compound with prostaglandin antagonist activity, related to one of the above structures. In another embodiment, the prostaglandin antagonist is any other prostaglandin antagonist known in the art.
Each prostaglandin antagonist represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject or the scalp thereof with a compound or composition capable of decreasing a level of a prostaglandin D2, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment, the compound or composition decreases the prostaglandin D2 level in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject or the scalp thereof with a compound or composition capable of inhibiting accumulation of a prostaglandin D2, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment, the compound or composition inhibits accumulation of the prostaglandin D2 level in the target scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the compound or composition is 2-(2′-benzothiazolyl)-5-styryl-3-(4′-phthalhydrazidyl)tetrazolium chloride (BSPT). In another embodiment, the compound or composition is any other PGD2S inhibitor known in the art. In another embodiment, the compound or composition inhibits brain type PGD2S. In another embodiment, the compound or composition inhibits hematopoietic type PGD2S. In another embodiment, the compound or composition inhibits both PGD2S types. In another embodiment, the compound or composition functions by any other means known in the art of inhibiting a PGD2S activity. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the compound acts upstream of PGD2S. In another embodiment, the compound inhibits early arachadonic acid metabolism. In another embodiment, the compound inhibits conversion of arachidonic acid to prostaglandin H2. In another embodiment, the compound or composition functions by inhibiting an activity of a cyclo-oxygenase (COX) enzyme. In another embodiment, the compound or composition inhibits a COX-1 enzyme. In another embodiment, the compound or composition inhibits a COX-2 enzyme. In another embodiment, the compound or composition inhibits both COX-1 and COX-2 enzymes. In another embodiment, the compound is a non-steroidal anti-inflammatory drug (NSAID). In another embodiment, the NSAID is SOLARAZE® (diclofenac topical). In another embodiment, the NSAID is any other NSAID known in the art. In another embodiment, the compound is a pgh2 analogue. In another embodiment, the compound or composition functions by any other means known in the art of decreasing prostaglandin D2 levels. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a topical NSAID used in methods and compositions of the present invention inhibits PGD2. In another embodiment, the NSAID, in addition to inhibiting PGD2, inhibits a pro-hair growth prostaglandin. In another embodiment, the NSAID is administered in conjunction with a pro-hair growth prostaglandin. In another embodiment, supplementation with a pro-hair growth prostaglandin inhibits anti-hair growth side effects of the NSAID. In another embodiment, the pro-hair growth prostaglandin is pge. In another embodiment, the pro-hair growth prostaglandin is a compound related to pge. In another embodiment, the pro-hair growth prostaglandin is pgf. In another embodiment, the pro-hair growth prostaglandin is a compound related to pgf. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
The COX enzyme that is the target of methods and compositions of the present invention is, in another embodiment, a prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (a.k.a. PTGS2) enzyme. In another embodiment, the COX enzyme is a COX-2 (COX2) enzyme. In another embodiment, the COX enzyme is a PGG/HS enzyme. In another embodiment, the COX enzyme is a PGHS-2 enzyme. PGHS-2 is, in another embodiment, a precursor of PGD2. In another embodiment, the COX enzyme is a PHS-2 enzyme. In another embodiment, the COX enzyme is a hCox-2 enzyme.
In another embodiment, the sequence of the target COX-2 enzyme is:
MPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGP QYCRSDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK LRAPIRSVTYTLAQLPCASMALQILWEAARHL (SEQ ID No: 273; GenBank Accession No. NM—004878). In another embodiment, the COX-2 enzyme is a homologue of SEQ ID No: 273. In another embodiment, the COX-2 enzyme is a variant of SEQ ID No: 273. In another embodiment, the COX-2 enzyme is an isomer of SEQ ID No: 273. In another embodiment, the COX-2 enzyme is a fragment of the protein having a sequence set forth in SEQ ID No: 273. Each possibility represents another embodiment of the present invention.
In another embodiment, the COX-2 enzyme has an amino acid (AA) sequence set forth in one of the following GenBank entries: NM—000963, BC013734, AJ634912, M90100. In another embodiment, the COX-2 enzyme has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the COX-2 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a beta-trace protein. In another embodiment, the COX-2 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a prostaglandin-H2 D-isomerase protein. In another embodiment, the COX-2 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a glutathione-independent PGD synthase protein. In another embodiment, the COX-2 enzyme is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-2 enzyme is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-2 enzyme is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-2 enzyme is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-2 enzyme comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the COX enzyme is a prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) (a.k.a. PTGS1) enzyme. In another embodiment, the COX enzyme is a COX1 enzyme. In another embodiment, the COX enzyme is a COX3 enzyme. In another embodiment, the COX enzyme is a PCOX1 enzyme. In another embodiment, the COX enzyme is a PGG/HS enzyme. In another embodiment, the COX enzyme is a PGHS-1 enzyme. In another embodiment, the COX enzyme is a PGHS1 enzyme. In another embodiment, the COX enzyme is a PHS1 enzyme. In another embodiment, the COX enzyme is a PTGHS enzyme.
In another embodiment, the sequence of the target COX-1 enzyme is:
MSRSLLLWFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQCD CTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWEFVNATFEREMLMRLVLTVRSNLI PSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDP QGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGKLKYQ VLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLYATLWLREHNRVCDLLKAE HPTWGDEQLFQTTRLILIGETIKIVIEEYVQQLSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYH WHPLMPDSFKIGGGRNMDHHILHVAVDVIRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEM AAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFGGEVG FNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTEL (SEQ ID No: 272; GenBank Accession No. NM—080591). In another embodiment, the COX-1 enzyme is a homologue of SEQ ID No: 272. In another embodiment, the COX-1 enzyme is a variant of SEQ ID No: 272. In another embodiment, the COX-1 enzyme is an isomer of SEQ ID No: 272. In another embodiment, the COX-1 enzyme is a fragment of the protein having a sequence set forth in SEQ ID No: 272. Each possibility represents another embodiment of the present invention.
In another embodiment, the COX-1 enzyme has an AA sequence set forth in one of the following GenBank entries: NM—000962, DQ180742, DQ180741, and DQ180740. In another embodiment, the COX-1 enzyme has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the COX-1 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a beta-trace protein. In another embodiment, the COX-1 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a prostaglandin-H2 D-isomerase protein. In another embodiment, the COX-1 enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a glutathione-independent PGD synthase protein. In another embodiment, the COX-1 enzyme is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-1 enzyme is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-1 enzyme is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-1 enzyme is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the COX-1 enzyme comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of the present invention functions by reducing the amount of PGHS-2 in a subject.
In another embodiment, demonstration in the present invention of up-regulation of the brain (lipocalin) form of PGD2S does not reflect the presence of mast cells (which express, in another embodiment, a distinct isoform of PGD2S). Rather, this finding identifies a separate mechanism for AGA. In another embodiment, both brain and hematopoietic forms are up-regulated.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject or the scalp thereof with a compound or composition capable of inhibiting an activity of a prostaglandin D2 synthase (PGD2S) enzyme, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment, the compound or composition inhibits the prostaglandin D2 synthase activity in the target scalp. In another embodiment, inhibiting PGD2S activity results in a decrease of prostaglandin D2 levels. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the PGD2S inhibitor is indomethacin. In another embodiment, the PGD2S inhibitor is a tetravalent selenium compound. In another embodiment, the PGD2S inhibitor is HQL-79. In another embodiment, the PGD2S inhibitor is specific for lipocalin PGD2S. In another embodiment, the PGD2S inhibitor inhibits more than 1 isoform of PGD2S. In another embodiment, the present invention utilizes a PGD2S inhibitor used to treat allergies. In another embodiment, the present invention utilizes a PGD2S inhibitor used to treat asthma. In another embodiment, the present invention utilizes a PGD2S inhibitor used to inhibit sleep.
In another embodiment, the PGD2S inhibitor has the structure:




wherein n is 0 or 1; R.sup.1 is hydrogen or halogen; R.sup.2 is halogen, cyano, C.sub.1-3alkylsulfonyl or trifluoromethyl; R.sup.3 is C.sub.1-3alkyl optionally substituted with 15 halogen atoms; and R.sup.4a and R.sup.4b are each hydrogen or one is hydrogen and the other is hydroxy, or both together represent oxo; with the proviso that when R.sup.1 is hydrogen, R.sup.2 is not 4-chloro.
In one embodiment of formula I are compounds wherein R.sup.4a and R.sup.4b are each hydrogen.
In a second embodiment of formula I are compounds wherein R.sup.1 is hydrogen and R.sup.2 is trifluoromethyl.
In a third embodiment of formula I are compounds wherein R.sup.3 is methyl.
In a fourth embodiment of formula I are compounds wherein R.sup.1 and R.sup.2 are independently a halogen atom.
In a fifth embodiment of formula I are compounds wherein n is 1.
In a sixth embodiment of formula I are compounds wherein n is 1, R.sup.3 is CH.sub.3, and R.sup.4a and R.sup.4b are each hydrogen.
In a seventh embodiment of formula I are compounds wherein n is 1, R.sup.3 is CH.sub.2F or CHF.sub.2, and R.sup.4a and R.sup.4b are each hydrogen.
In an eighth embodiment of formula I are compounds wherein n is 1, R.sup.1 is hydrogen, R.sup.3 is methyl, R.sup.4a and R.sup.4b are each hydrogen, and R.sup.2 is 4-cyano, 4-methanesulfonyl or 4-trifluoromethyl.
In another embodiment, the PGD2S inhibitor is ((1R)-6-fluoro-8-(methylsulfonyl)-9-{(1S)-1-[4-(trifluoromethyl)phenyl]ethyl}-2,3,4,9-tetrahydro-1H-carbazol-1-yl)acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(3,4-dichlorophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is {(1R)-6-fluoro-8-(methylsulfonyl)-9-[(1S)-1-phenylethyl]-2,3,4,9-tetrahydro-1H-carbazol-1-yl}acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-6-fluoro-9-[(1S)-1-(4-fluorophenyl)ethyl]-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(4-chloro-3-fluorophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(3-chlorophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(4-chloro-2-fluorophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(4-bromophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is [(1R)-9-[(1S)-1-(4-cyanophenyl)ethyl]-6-fluoro-8-(methylsulfonyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is ((1R)-6-fluoro-8-(methylsulfonyl)-9-{((1S)-1-[4-(methylsulfonyl)phenyl]ethyl}-2,3,4,9-tetrahydro-1H-carbazol-1-yl)acetic acid. In another embodiment, the PGD2S inhibitor is (1R)-1-[4-(trifluoromethyl)phenyl]ethanol. In another embodiment, the PGD2S inhibitor is 1-(4-chloro-2-fluorophenyl)ethanone. In another embodiment, the PGD2S inhibitor is 1-(4-chloro-3-fluorophenyl)ethanone. In another embodiment, the PGD2S inhibitor is a pharmaceutically acceptable salt of one of the above compounds.
In another embodiment, the PGD2S inhibitor is 2-[(1R)-9-(4-chlorobenzyl)-8-((R)-methylsulfinyl)-2,-3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid and 2-[(1R)-9-(4-chlorobenzy-1)-8-((S)-methylsulfinyl-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid.
In another embodiment, the PGD2S inhibitor has one of the following formulas:




In another embodiment, the PGD2S inhibitor is 2-[(1R)-9-(4-chlorobenzyl)-8-((S)-methylsulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is substantially pure 2-[(1R)-9-(4-chlorobenzyl)-8-((S)-methylsulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid.
In another embodiment, the PGD2S inhibitor is 2-[(1R)-9-(4-chlorobenzyl)-8-((R)-methylsulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid. In another embodiment, the PGD2S inhibitor is substantially pure 2-[(1R)-9-(4-chlorobenzyl)-8-((R)-methylsulfinyl)-2,3,4,9-tetrahydro-1H-carbazol-1-yl]acetic acid.
In another embodiment, the PGD2S inhibitor has the formula:




wherein:





represents





R represents hydrogen, alkyl, alkoxy, halogen, hydroxy, acyloxy, or optionally substituted arylsulfonyloxy, X represents hydrogen or alkyl, and the double bond on the □-chain has E configuration or Z configuration, provided that the compound of the formula





wherein R1a represents hydrogen, alkyl, or alkoxy, X is as defined above, and the double bond on the □-chain has E configuration or Z configuration is excluded.

In another embodiment, the PGD2S inhibitor is a pharmaceutically acceptable salt of one of the above PGD2S inhibitors.
In another embodiment, the PGD2S inhibitor is any other PGD2S inhibitor known in the art. Each PGD2S inhibitor represents a separate embodiment of the present invention.
In another embodiment, a compound utilized in the present invention is a CRTH2 antagonist.
In another embodiment, the CRTH2 antagonist has the structure:




    
    
        wherein:
    
    


R.sup.1 is a 1,3-benzothiazole group optionally substituted by halogen, C.sub.1-6alkyl, C.sub.1-6alkoxy or a group of formula (A) or (B):




wherein R.sup.4 and R.sup.5 are independently halogen, C.sub.1-6allyl, C.sub.1-6alkoxy, phenoxy optionally substituted by halogen, C.sub.1-6alkyl, or C.sub.1-6alkoxy





    
    
        wherein one of X and Y is nitrogen and the other is nitrogen, oxygen or sulphur and R.sup.6 is phenyl optionally substituted by substituted by halogen, C.sub.1-6allyl, or C.sub.1-6alkoxy;
    
    
    R.sup.2 is hydrogen, halogen, C.sub.1-6-alkyl, C.sub.1-6alkoxy, and
    R.sup.3 is hydrogen or C.sub.1-6alkyl.


In another embodiment, “alkyl” includes straight chain and branched chain alkyl groups.
In another embodiment, R.sup.1 of the above formula is a 1,3-benzothiazole group, or a group of formula (A). In another embodiment, the groups R.sup.4 and R.sup.5 can be the same or different. In another embodiment, R.sup.4 and R.sup.5 are both propoxy, chloro or phenoxy.
In another embodiment, the CRTH2 antagonist is [1-(2,6-diphenoxypyrimidin-4-yl)-2,5-dimethyl-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is [1-(2,6-diphenoxypyrimidin-4-yl)-5-methoxy-2-methyl-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is [1-(2,6-diisopropoxypyrimidin-4-yl)-2,5-dimethyl-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is [5-methoxy-2-methyl-1-(6-methyl-2-phenylpyrimidin4-yl)-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is [1-(2,6-dichloropyrimidin-4-yl)-2,5-dimethyl-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is [1-(1,3-benzothiazol-2-yl)-5-methoxy-2-methyl-1H-indol-3-yl]acetic acid. In another embodiment, the CRTH2 antagonist is a pharmaceutically acceptable salt of one of the above compounds.
In another embodiment, the CRTH2 antagonist has the structure:




    
    
        wherein:
    
    
    R.sup.1 is hydrogen, halogen, C.sub.1-6alkyl, or C.sub.1-6alkoxy;
    R.sup.2 is hydrogen, halogen, C.sub.1-6alkyl, or C.sub.1-6alkoxy;
    R.sup.3 is hydrogen or C.sub.1-6alkyl;
    R.sup.4 is hydrogen, C.sub.1-6alkyl, C.sub.1-6alkoxy, or thioC.sub.1-6alkyl; and
    X is N or CH.


In another embodiment, “alkyl” includes straight chain and branched chain alkyl groups.
In another embodiment, R.sup.1 in the above formula is hydrogen, chloro or methyl.
In another embodiment, R.sup.2 is methyl, iso-propyl or methoxy.
In another embodiment, R.sup.4 is hydrogen or methoxy.
In another embodiment, X is CH.
In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 5-methoxy-2-methyl-1-(4-quinazolinyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 2-methyl-1-(2-methyl-4-quinazolinyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(6-chloro-2-quinolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(6,8-dichloro-4-quinazolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(4-chloro-2-quinolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 2,5-dimethyl-1-(4-quinazolinyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 2,5-dimethyl-1-(4-quinazolinyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-5-fluoro-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 5-methoxy-2-methyl-1-[2-(methylthio)-4-quinazolinyl]-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 5-methoxy-1-(6-methoxy-4-quinolinyl)-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 2-methyl-1-[2-(methylthio)-4-quinazolinyl]-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-[2-(ethylthio)-4-quinazolinyl]-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-2,5-dimethyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-2-methyl-5-(1-methylethyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 2,5-dimethyl-1-[2-(methylthio)-4-quinazolinyl]-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinazolinyl)-2-methyl-5-(2-methylpropoxy)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinolinyl)-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-2-methyl-4-quinolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 5-methoxy-2-methyl-1-(7-methyl-4-quinolinyl)-1H-indole-3-acetic acid. In another embodiment, the CRTH2 antagonist is 1-(7-chloro-4-quinolinyl)-5-methoxy-2-methyl-1H-indole-3-acetic acid.
In another embodiment, the CRTH2 antagonist is a pharmaceutically acceptable salt of one of the above CRTH2 antagonists.
In another embodiment, the CRTH2 antagonist is any other CRTH2 antagonist known in the art. Each CRTH2 antagonist represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating baldness in a scalp of a subject, comprising the step of contacting the subject or the scalp thereof with a compound or composition capable of decreasing a level of a prostaglandin D2 synthase enzyme, thereby treating baldness in a scalp of a subject. In another embodiment, the baldness is mediated by an AGA. In another embodiment, the compound or composition decreases the prostaglandin D2 synthase level in the target scalp. Each possibility represents a separate embodiment of the present invention.
The compound or composition utilized in methods and compositions of the present invention is, in another embodiment, an antisense nucleotide molecule. In another embodiment, the compound or composition is an RNA inhibitory molecule. In another embodiment, the compound or composition is an RNA molecule. In another embodiment, the compound or composition is an inhibitory RNA (RNAi) molecule. In another embodiment, the RNA molecule is a short hairpin RNA (shRNA). In another embodiment, the RNA molecule is a small inhibitory RNA (siRNA). In another embodiment, the RNA molecule is a microRNA (miRNA). The use of siRNA and miRNA has been described, inter alia, in (Caudy A A et al, Genes & Devel 16: 2491-96 and references cited therein). In another embodiment, the RNA molecule is an anti-sense locked-nucleic acid (LNA) oligonucleotide. In another embodiment, the RNA molecule is any type of inhibitory RNA enumerated or described in Banan M et al (The ins and outs of RNAi in mammalian cells. Curr Pharm Biotechnol. 2004 October; 5(5):441-50. In another embodiment, the RNA molecule is any type of RNAi known in the art.
In another embodiment, an antisense nucleotide or RNA inhibitory molecule (of any of the types mentioned above) hybridizes with a nucleotide molecule encoding a prostaglandin D2 synthase enzyme. In another embodiment, the antisense nucleotide or RNA inhibitory molecule induces degradation of a nucleotide molecule encoding a prostaglandin D2 synthase enzyme. In another embodiment, the antisense nucleotide or RNA inhibitory molecule hybridizes with a nucleotide molecule encoding a protein selected from CCL18, Col11 A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. In another embodiment, the antisense nucleotide or RNA inhibitory molecule induces degradation of a nucleotide molecule encoding a protein selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the antisense nucleotide or RNA inhibitory molecule induces degradation of a nucleotide molecule encoding a protein selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. In another embodiment, the nucleotide molecule is a mRNA. In another embodiment, the nucleotide molecule is a transcript. In another embodiment, the nucleotide molecule is a genomic sequence. Each possibility represents a separate embodiment of the present invention.
Methods for topical delivery of antisense nucleotides are well known in the art, and are described, for example, in Jiang M et al (Gel-based application of siRNA to human epithelial cancer cells induces RNAi-dependent apoptosis. Oligonucleotides. 2004 Winter; 14(4):239-48) and in de Jonge J et al (Reconstituted influenza virus envelopes as an efficient carrier system for cellular delivery of small-interfering RNAs. Gene Ther. 2006 March; 13(5):400-11). Each method represents a separate embodiment of the present invention.
The PGD2S enzyme targeted by methods and compositions of the present invention is, in another embodiment, a lipocalin PGD2S enzyme. In another embodiment, the PGD2S enzyme is a hematopoietic PGD2S enzyme. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the PGD2S enzyme has the sequence:

    MATHHTLWMGLALLGVLGDLQAAPEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKK AALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSV VETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQ TDKCMTEQ (SEQ ID No: 252). In another embodiment, the PGD2S enzyme is a homologue of SEQ ID No: 252. In another embodiment, the PGD2S enzyme is a variant of SEQ ID No: 252. In another embodiment, the PGD2S enzyme is an isomer of SEQ ID No: 252. In another embodiment, the PGD2S enzyme is a fragment of the protein having a sequence set forth in SEQ ID No: 252. Each possibility represents another embodiment of the present invention.


In another embodiment, the PGD2S enzyme has the sequence:

    MATHHTLWMGLALLGVLGDLQAAPEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKK AALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSV VETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQ TDKCMTEQ (SEQ ID No: 267). In another embodiment, the PGD2S enzyme is a homologue of SEQ ID No: 267. In another embodiment, the PGD2S enzyme is a variant of SEQ ID No: 267. In another embodiment, the PGD2S enzyme is an isomer of SEQ ID No: 267. In another embodiment, the PGD2S enzyme is a fragment of the protein having a sequence set forth in SEQ ID No: 267. Each possibility represents another embodiment of the present invention.


In another embodiment, the PGD2S enzyme has an amino acid (AA) sequence set forth in one of the following GenBank entries: NM—000954, AK075333, BC005939, BC041463, M61900, M61901, BC005939, BC041463, AY026356, BT019921, and BT019922, AL807752, CAI12758, DQ297141, ABB84464, M98538, AAB51074, M98539, AA621632, AI659247, AAK07679, AAH05939, AAH41463, BG037037, BM686612, AAV38724, AAV38725, CR594734, CR610092, CR612267, CR614437, AAA36494, and AAA36496. In another embodiment, the PGD2S enzyme has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the PGD2S enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a beta-trace protein. In another embodiment, the PGD2S enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a prostaglandin-H2 D-isomerase protein. In another embodiment, the PGD2S enzyme has a nucleotide or AA sequence set forth in a GenBank entry for a glutathione-independent PGD synthase protein. In another embodiment, the PGD2S enzyme is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the PGD2S enzyme is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the PGD2S enzyme is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the PGD2S enzyme is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the PGD2S enzyme comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of the present invention functions by reducing an amount of lipocalin PGD2S (L-PGD2) in a subject. In another embodiment, a method of the present invention functions by inactivating the L-PGD2 or a fraction thereof in a subject.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing an activity of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the compound or composition increases the target gene activity in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing an expression of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the compound or composition increases the target gene expression in the target scalp or skin. In another embodiment, the compound or composition increases the level of a protein encoded by the target gene in the target scalp or skin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a protein product of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, a mimetic of the protein product is administered. In another embodiment, an analogue of the protein product is administered. In another embodiment, the compound or composition increases the protein product concentration in the target scalp or skin. In another embodiment, the protein product is FGF18. In another embodiment, the protein is a product of a target gene selected from GPRC5D, GPR49, LRRC15, Serpin A, and CDT6. In another embodiment, the protein is a product of a target gene is selected from BMP2, LHX2, THBS1, MYCN, NR4A2, MEST, TM4SF1, CRLF1, TNFRSF12A, SELENBP1, GPR161, HEPH, FZD7, and CLIC4. In another embodiment, the protein product is a product of any target gene of the present invention. Each possibility represents a separate embodiment of the present invention.
The compound or composition capable of increasing an expression of a target protein that is utilized in a method or composition of the present invention, is, in another embodiment, a nucleotide encoding the target protein. In another embodiment, the compound or composition positively regulates expression of the gene encoding the target protein. In another embodiment, the compound or composition positively regulates transcription of the gene encoding the target protein. In another embodiment, the compound or composition positively regulates translation of an RNA molecule encoding the target protein. In another embodiment, the compound or composition positively regulates expression of the target protein in a post-translational fashion. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the target gene of methods and compositions of the present invention is FGF18. In another embodiment, the target gene is GPRC5D. In another embodiment, the target gene is GPR49. In another embodiment, the target gene is LRRC15. In another embodiment, the target gene is Serpin A. In another embodiment, the target gene is BMP2. In another embodiment, the target gene is LHX2. In another embodiment, the target gene is THBS1. In another embodiment, the target gene is MYCN. In another embodiment, the target gene is NR4A2. In another embodiment, the target gene is MEST. In another embodiment, the target gene is TM4SF1. In another embodiment, the target gene is CRLF1. In another embodiment, the target gene is TNFRSF12A. In another embodiment, the target gene is SELENBP1. In another embodiment, the target gene is GPR161. In another embodiment, the target gene is HEPH. In another embodiment, the target gene is FZD7. In another embodiment, the target gene is CLIC4. In another embodiment, the target gene is CDT6. In another embodiment, the target gene is another gene having a sequence selected from the sequences set forth in SEQ ID No: 1-251. In another embodiment, the target gene is another gene having a sequence selected from the sequences set forth in SEQ ID No: 277-303. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the target gene is a gene from FIG. 13. In another embodiment, a cell surface protein from FIG. 13 is utilized for isolating subpopulations of cells for tissue engineering. In another embodiment, a signaling molecule from FIG. 13 is utilized as a drug target. In another embodiment, a growth factor from FIG. 13 is utilized as a target protein. In another embodiment, a transcription factor from FIG. 13 is utilized is utilized as a target protein. In another embodiment, a cytokine from FIG. 13 is utilized is utilized as a target protein. In another embodiment, a G-protein-coupled receptor from FIG. 13 is utilized is utilized as a target protein. In another embodiment, a protein from FIG. 1.3 with a biological activity related to miniaturization of the hair follicle in baldness is utilized as a target protein.
As provided herein, findings of the present invention demonstrate that various target genes (e.g. FGF18, GPRC5D, GPR49, LRRC15, CDT6, Serpin A, BMP2, LHX2, THBS1, MYCN, NR4A2, MEST, TM4SF1, CRLF1, TNFRSF12A, SELENBP1, GPR161, HEPH, FZD7, and CLIC4) are up-regulated in haired scalp. Thus, increasing expression or activity of products of these genes in bald scalp will stimulate hair growth. The common categories listed in FIG. 4D for the haired scalp exemplified genes that are enriched in a sample of anagen and terminal HF, relative to telogen and vellus HF (FIG. 1). This analysis therefore determined genes enriched in human anagen and terminal follicles.
Many of the genes of highest significance were related to inflammation. The 3 most populated gene function categories are response to biotic stimulus, defense response and immune response with p values ranging from 1.81×10−2 to 2.67×10−2. The category most commonly represented—response to biotic stimulus—is defined, in another embodiment, as: “A change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a biotic stimulus, a stimulus caused or produced by a living organism” (Hill D P, Begley D A, Finger J H, Hayamizu T F, McCright I J, Smith C M, Beal J S, Corbani L E, Blake J A, Eppig J T, Kadin J A, Richardson J E, Ringwald M. 2004. The mouse Gene Expression Database (GXD): updates and enhancements. Nucleic Acids Res 32: D568-D571).
Further, bald scalp exhibited a relative decrease in the proportion of alpha-6 integrin negative cells within the stem cell compartment. This is due, in another embodiment, to a decrease in the number of cell layers of the outer root sheath as the follicle miniaturizes. Since the suprabasal alpha-6 integrin negative bulge population is likely to be a progeny population from the basal layer, these findings show that, under the conditions utilized herein, haired scalp possesses a higher state of activation and division in the basal layer stem cell compartment compared with bald scalp.
Thus, findings of the present invention reveal genes that participate in hair growth, healthy HF maintenance and cycling, and hair loss, that can be used as targets of method of the present invention.
The FGF18 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 253)







MYSAPSACTCLCLHFLLLCFQVQVLVAEENVDFRIHVENQTRARDDVSRK


 


QLRLYQLYSRTSGKHIQVLGRRISARGEDGDKYAQLLVETDTFGSQVRIK


 


GKETEFYLCMNRKGKLVGKPDGTSKECVFIEKVLENNYTALMSAKYSGWY


 


VGFTKKGRPRKGPKTRENQQDVHFMKRYPKGQPELQKPFKYTTVTKRSRR


 


IRPTHPA.






In another embodiment, the FGF18 protein has an AA sequence set forth in one of the following GenBank entries: NM—003862, BC006245, AF075292, BC006245, NM—033649, AF211188, AY358811, BT019570, BT019571, and AB007422. In another embodiment, the FGF18 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the FGF18 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the FGF18 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the FGF18 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the FGF18 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the FGF18 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The GPRC5D protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:

    MYKDCIESTGDYFLLCDAEGPWGITLESLAILGIVVTILLLLAFLFLMRIQDCSQWNVLPTQL LFLLSVLGLFGALFAFIIELNQQTAPVRYFLFGVLFALCFCLLAHASNLVKLVRGCVSFSWTT ILCIAIGCSLLQIHATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATF CGPCENWKQHGRLIFITVLFSIIIVVVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFL LLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGT PIQPQTVDPTQECFIPQAKLSPQQDAGGV (SEQ ID No: 254). In another embodiment, the GPRC5D protein is encoded by a gene located at 12p13.3.


In another embodiment, the GPRC5D protein has an AA sequence set forth in one of the following GenBank entries: NM—018654, BC069341, BC107077, BC107078, AF209923, BC069341, and AB099817. In another embodiment, the GPRC5D protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the GPRC5D protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPRC5D protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPRC5D protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPRC5D protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPRC5D protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The GPR49 protein targeted by methods and compositions of the present invention is a protein that would be encoded, in another embodiment, by a gene having the sequence:

    ttatwahaaatttatuttaacccaatagaaaagcaaataggaatctatttacaagtactatatatttacatatatacagttagagtgggagatttaaaga aaatgggcagagaaacacaatataaatcaaagaatatgccactgtacaaggcattattatcattatcatggtccttaatgttactgaaccutactatagt aataaatacaguctatatttacacatcttataaaacatctcataaatgtattuttcaaatccaagthaaaacatctgatcaaaataaacatgcttatataaa aataaatctacctaacagccatttggtttggatgtattgargctaatataggataatagagggtaagrbttaatactttgacttttcttatttaataacttgctt cttaaaatacctaacacagtattaatatggaatargcrgagargtaatgttcctaacatcaagtgggttatccagagagaacacagctaaaaccaagc taaataaacaggataitacgttactgagtctatgagtccaaagtggtgtcagatattgggtagccagagctactagagatacatgtgtgagaggttg tatcagtggacttaatttatgtgatgtgcacatttgatcattaagatgcacatcaghtgaatcaactgataaaacttattgcaaaaattctttactaaccca gaaaaaaaatcccagattgcttactucttttccaggtatgtycattgctggcagtggaattccatctgagctttgggcmcaaggagttaaaaacaaa tcagataagacatacgtcacctgtscatgattscatagtaacaatttaagaattuggtcagtattctttcaaaatacttgtaagcaguttatcccatgak ggtggaccatctagtgctgatacataaamaggtatctctaaaawtgatctcaatatgagtgagtaacaatacytwacattaccayctaagggattg tscttagaaggatctttc (SEQ ID No: 255). In another embodiment, the GPR49 protein is encoded by a gene located at 12q22-q23.


In another embodiment, the GPR49 protein has an AA sequence set forth in one of the following GenBank entries: AK075399, BC096324, BC096325, BC099650, BC096326, AF062006, and AF061444. In another embodiment, the GPR49 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the GPR49 protein has an AA or nucleotide sequence set forth in a GenBank entry for an LGF5 protein. In another embodiment, the GPR49 protein has an AA or nucleotide sequence set forth in a GenBank entry for an HG38 protein. In another embodiment, the GPR49 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR49 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR49 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR49 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR49 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The LRRC15 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 256)







MPLKHYLLLLVGCQAWGAGLAYHGCPSECTCSRASQVECTGARIVAVPTP


 


LPWNAMSLQILNTHITELNESPFLNISALIALRIEKNELSRITPGAFRNL


 


GSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLLQIQPAHFSQCSN


 


LKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQ


 


VLRLYENRLTDIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRL


 


YLSNNHISQLPPSIFMQLPQLNRLTLFGNSLKELSLGIFGPMPNLRELWL


 


YDNHISSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHT


 


NALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMAIQLQNNQ


 


LENLPLGIFDHLGKLCELRLYDNPWRCDSDILPLRNWLLLNQPRLGTDTV


 


PVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETPWYPDTPSYPDTT


 


SVSSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSGLAIAAIVIGIVA


 


LACSLAACVGCCCCKKRSQAVLMQMKAPNEC.






In another embodiment, the LRRC15 protein has an AA sequence set forth in one of the following GenBank entries: AB071037, BC101064, BC101065, and BK001325. In another embodiment, the LRRC15 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the LRRC15 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the LRRC15 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the LRRC15 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the LRRC15 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the LRRC15 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CDT6 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 257)









MLKKPLSAVTWLCIFIVAFVSHPAWLQKLSKHKTPAQPQLKAANCCEEVKELKAQVANLSS



 


LLSELNKKQERDWVSVVMQVMELESNSKRMESRLTDAESKYSEMNNQIDIMQLQAAQTVT


 


QTSADAIYDCSSLYQKNYRISGVYKLPPDDFLGSPELEVFCDMETSGGGWTIIQRRKSGLVSF


 


YRDWKQYKQGFGSIRGDFWLGNEHIHRLSRQPTRLRVEMEDWEGNLRYAEYSHFVLGNEL


 


NSYRLFLGNYTGNVGNDALQYHNNTAFSTKDKDNDNCLDKCAQLRKGGYWYNCCTDSNL


 


NGVYYRLGEHNKHLDGITWYGWHGSTYSLKRVEMKIRPEDFKP.






In another embodiment, the CDT6 protein has an AA sequence set forth in one of the following GenBank entries: BC001881, NM—021146, AY358301, Y16132, and BT009802. In another embodiment, the CDT6 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CDT6 protein has an AA or nucleotide sequence set forth in a GenBank entry for an ANGPTL7 (Angiopoietin-like 7) protein. In another embodiment, the CDT6 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDT6 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDT6 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDT6 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDT6 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The Serpin A protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 258)









MPSSVSWGILLLAGLCCLVPVSLAEDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLY



 


RQLAHQSNSTNIFFSPVSIATAFAMLSLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTL


 


NQPDSQLQLTTGNGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKG


 


TQGKIVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVDQATTVKVPMMKRL


 


GMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLENELTHDIITKFLENEDRRSASLH


 


LPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEAAG


 


AMFLEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK.






In another embodiment, the Serpin A protein has an AA sequence set forth in one of the following GenBank entries: BC015642, BC011991, NM—000295, BC070163, AK026174, X01683, NM—001002236, M11465, NM—001002235, V00496, X02920, and K01396. In another embodiment, the Serpin A protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the Serpin A protein is a Serpin A1 protein. In another embodiment, the Serpin A protein is any other Serpin A protein known in the art. In another embodiment, the Serpin A protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the Serpin A protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the Serpin A protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the Serpin A protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the Serpin A protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The BMP2 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 304; GenBank Accession No. BC140325)









MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFG



 


LKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPET


 


SGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVT


 


RLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRS


 


LHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVG


 


WNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISM


 


LYLDENEKVVLKNYQDMVVEGCGCR.






In another embodiment, the BMP2 protein has an AA sequence set forth in one of the following GenBank entries: BC069214, NM—001200, M22490, M22489, and AY418812. In another embodiment, the BMP2 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the BMP2 protein is any other BMP2 protein known in the art. In another embodiment, the BMP2 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the BMP2 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the BMP2 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the BMP2 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the BMP2 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The LHX2 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 305; GenBank Accession No. NM_004789)









MLFHSLSGPEVHGVIDEMDRRAKSEAPAISSAIDRGDTETTMPSISSDRAALCAGCGGKISDRYY



 


LLAVDKQWHMRCLKCCECKLNLESELTCFSKDGSIYCKEDYYRRFSVQRCARCHLGISASEMV


 


MRARDLVYHLNCFTCTTCNKMLTTGDHFGMKDSLVYCRLHFEALLQGEYPAHFNHADVAAA


 


AAAAAAAKSAGLGAAGANPLGLPYYNGVGTVQKGRPRKRKSPGPGADLAAYNAALSCNEND


 


AEHLDRDQPYPSSQKTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLAQKTGLTKRVLQV


 


WFQNARAKFRRNLLRQENTGVDKSTDAALQTGTPSGPASELSNASLSPSSTPTTLTDLTSPTLPT


 


VTSVLTSVPGNLEGHEPHSPSQTTLTNLF.






In another embodiment, the LHX2 protein has an AA sequence set forth in one of the following GenBank entries: AL158052, BC093662, BC112185, and AF124735. In another embodiment, the LHX2 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the LHX2 protein is any other LHX2 protein known in the art. In another embodiment, the LHX2 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the LHX2 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the LHX2 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the LHX2 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the LHX2 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The THBS1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 306; GenBank Accession No. NM_003246)









MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIE



 


DANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNG


 


KAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQ


 


SVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVV


 


NGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANE


 


LRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRC


 


WPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFK


 


QDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGG


 


WGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNP


 


CFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGY


 


NCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYR


 


CECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIG


 


DACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGE


 


GDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIG


 


DTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRL


 


VPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQND


 


PNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRF


 


YVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTL


 


WHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVF


 


SQEMVFFSDLKYECRDP.






In another embodiment, the THBS1 protein has an AA sequence set forth in one of the following GenBank entries: X14787, M14326, AY405252, BC015134, and M25631. In another embodiment, the THBS1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the THBS1 protein is any other THBS1 protein known in the art. In another embodiment, the THBS1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the THBS1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the THBS1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the THBS1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the THBS1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The MYCN protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 307; GenBank Accession No. BC002712)









MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSR



 


GFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLER


 


AVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDP


 


AVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKAL


 


STSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSE


 


LILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERR


 


RNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKL


 


QARQQQLLKKIEHARTC.






In another embodiment, the MYCN protein has an AA sequence set forth in GenBank Accession Number NM—005378. In another embodiment, the MYCN protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the MYCN protein is any other MYCN protein known in the art. In another embodiment, the MYCN protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the MYCN protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the MYCN protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the MYCN protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the MYCN protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The NR4A2 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 308; GenBank Accession No. NM_006186)









MPCVQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITATTSLPSFSTFMD



 


NYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQHSHLPPQSEEMMPHSGSVYYKPSSPP


 


TPTTPGFQVQHSPMWDDPGSLHNFHQNYVATTHMIEQRKTPVSRLSLFSFKQSPPGTPVSSCQ


 


MRFDGPLHVPMNPEPAGSHHVVDGQTFAVPNPIRKPASMGFPGLQIGHASQLLDTQVPSPPSRG


 


SPSNEGLCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCLANKNCPVDKRRRNRCQ


 


YCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKSPQEPSPPSPPVSLISALVRAHVDSNPAMT


 


SLDYSRFQANPDYQMSGDDTQHIQQFYDLLTGSMEIIRGWAEKIPGFADLPKADQDLLFESAFL


 


ELFVLRLAYRSNPVEGKLIFCNGVVLHRLQCVRGFGEWIDSIVEFSSNLQNMNIDISAFSCIAALA


 


MVTERHGLKEPKRVEELQNKIVNCLKDHVTFNNGGLNRPNYLSKLLGKLPELRTLCTQGLQRIF


 


YLKLEDLVPPPAIIDKLFLDTLPF.






In another embodiment, the NR4A2 protein has an AA sequence set forth in one of the following GenBank entries: NM—173171, NM—173172, NM—173173, and X75918. In another embodiment, the NR4A2 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the NR4A2 protein is any other NR4A2 protein known in the art. In another embodiment, the NR4A2 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the NR4A2 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the NR4A2 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the NR4A2 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the NR4A2 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The MEST protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 309; GenBank Accession No. BC002413)









MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQD



 


SVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQA


 


SIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPL


 


LLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDS


 


LLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISH


 


YPQLEDPMGFLNAYMGFINSF.






In another embodiment, the MEST protein has an AA sequence set forth in one of the following GenBank entries: BC011908, BC014564, BC018695, BC090049, NM—002402, NM—177524, NM—177525, AK098397, D78611, D87367, and Y11534. In another embodiment, the MEST protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the MEST protein is any other MEST protein known in the art. In another embodiment, the MEST protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the MEST protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the MEST protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the MEST protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the MEST protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The TM4SF1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 310; GenBank Accession No. BC008442)









MCYGKCARCIGHSLVGLALLCIAANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLL



 


PAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDS


 


LGQWNYTFASTEGQYLLDTSTWSECTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGG


 


ICGFCCSHQQQYDC.






In another embodiment, the TM4SF1 protein has an AA sequence set forth in one of the following GenBank entries: AL832780, X75684, BC034145, AK093829, AK130073, and AK130271. In another embodiment, the TM4SF1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the TM4SF1 protein is any other TM4SF1 protein known in the art. In another embodiment, the TM4SF1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the TM4SF1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the TM4SF1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the TM4SF1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the TM4SF1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CRLF1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 311; GenBank Accession No. BC044634)









MPAGRRGPAAQSARRPPPLLPLLLLLCVLGAPRAGSGAHTAVISPQDPTLLIGSSLLATCSVH



 


GDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSIL


 


AGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTC


 


EEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRV


 


GGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTV


 


YFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELK


 


QFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARGPAR.









In another embodiment, the CRLF1 protein has an AA sequence set forth in one of the following GenBank entries: AF059293, AF073515, AF178684, NM—004750, and AY358291. In another embodiment, the CRLF1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CRLF1 protein is any other CRLF1 protein known in the art. In another embodiment, the CRLF1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CRLF1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CRLF1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CRLF1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CRLF1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The TNFRSF12A protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 312; GenBank Accession No. BC002718)







MARGSLRRLLRLLVLGLWLALLRSVAGEQAPGTAPCSRGSSWSADLD


 


KCMDCASCRARPHSDFCLGCAAAPPAPFRLLWPILGGALSLTFVLGLL


 


SGFLVWRRCRRREKFTTPIEETGGEGCPAVALIQ.






In another embodiment, the TNFRSF12A protein has an AA sequence set forth in one of the following GenBank entries: NM—016639, AB035480, and AF191148. In another embodiment, the TNFRSF12A protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the TNFRSF12A protein is any other TNFRSF12A protein known in the art. In another embodiment, the TNFRSF12A protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the TNFRSF12A protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the TNFRSF12A protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the TNFRSF12A protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the TNFRSF12A protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The SELENBP1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 313; GenBank Accession No. BC009084)









MATKCGNCGPGYSTPLEAMKGPREEIVYLPCIYRNTGTEAPDYLATVDVDPKSPQYCQVIH



 


RLPMPNLKDELHHSGWNTCSSCFGDSTKSRTKLVLPSLISSRIYVVDVGSEPRAPKLHKVIEP


 


KDIHAKCELAFLHTSHCLASGEVMISSLGDVKGNGKGGFVLLDGETFEVKGTWERPGGAAP


 


LGYDFWYQPRHNVMISTEWAAPNVLRDGFNPADVEAGLYGSHLYVWDWQRHEIVQTLSL


 


KDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKNEGGTWSVEKVIQVPPKKVKGWLLPEM


 


PGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLFLGGSIVKGGPVQVLEDEEL


 


KSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIREGSVMLQVDVD


 


TVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI.






In another embodiment, the SELENBP1 protein has an AA sequence set forth in one of the following GenBank entries: BC032997 and NM—003944. In another embodiment, the SELENBP1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the SELENBP1 protein is any other SELENBP1 protein known in the art. In another embodiment, the SELENBP1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the SELENBP1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the SELENBP1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the SELENBP1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the SELENBP1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The GPR161 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 314; GenBank Accession No. BC028163)









MSLNSSLSCRKELSNLTEEEGGEGGVIITQFIAIIVITIFVCLGNLVIVVTLYKKSYLLTLSNKFVFS



 


LTLSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGVIAIDRYYAVLYPMV


 


YPMKITGNRAVMALVYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWC


 


ALFPFLVMLVCYGFIFRVARVKARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRNAFQGV


 


VYSANQCKALITILVVLGAFMVTWGPYMVVIASEALWGKSSVSPSLETWATWLSFASAVCHPL


 


IYGLWNKTVRKELLGMCFGDRYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGH


 


SSSTGDTGFSCSQDSGTDMMLLEDYTSDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHV


 


KAEVHKSLDSYAASLAKAIEAEAKINLFGEEALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQL


 


QSIEEGDVLAAEQR.






In another embodiment, the GPR161 protein has an AA sequence set forth in one of the following GenBank entries: AK091271 and NM—153832. In another embodiment, the GPR161 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the GPR161 protein is any other GPR161 protein known in the art. In another embodiment, the GPR161 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR161 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR161 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR161 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR161 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The HEPH protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 315; GenBank Accession No. BC011561)









MESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFL



 


KSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIH


 


PHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVD


 


APRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASV


 


DKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRG


 


HHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKV


 


RQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQE


 


KMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPG


 


LVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGAD


 


GKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNL


 


PRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDN


 


LGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWE


 


REWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEV


 


GDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWI


 


YYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGS


 


QDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAE


 


SFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHL


 


SPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQ


 


HRQRKLRRNRRSILDDSFKLLSFKQ.






In another embodiment, the HEPH protein has an AA sequence set forth in one of the following GenBank entries: NM—138737, AF075034, AY358990, AB014598, AF148860, and NM—014799. In another embodiment, the HEPH protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the HEPH protein is any other HEPH protein known in the art. In another embodiment, the HEPH protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the HEPH protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the HEPH protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the HEPH protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the HEPH protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The FZD7 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 316; GenBank Accession No. AB017365)









MRDPGAAAPLSSLGLCALVLALLGALSAGAGAQPYHGEKGISVPDHGFCQPISIPLCTDIAYNQT



 


ILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQ


 


GCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYLPDLPFTA


 


LPPGASDGRGRPAFPFSCPRQLKVPPYLGYRFLGERDCGAPCEPGRANGLMYFKEEERRFARL


 


WVGVWSVLCCASTLFTVLTYLVDMRRFSYPERPIIFLSGCYFMVAVAHVAGFLLEDRAVCVER


 


FSDDGYRTVAQGTKKEGCTILFMVLYFFGMASSIWWVILSLTWFLAAGMKWGHEAIEANSQY


 


FHLAAWAVPAVKTITILAMGQVDGDLLSGVCYVGLSSVDALRGFVLAPLFVYLFIGTSFLLAGF


 


VSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVLYTVPATIVLACYFYEQAFREHWERTWLLQTC


 


KSYAVPCPPGHFPPMSPDFTVFMIKYLMTMIVGITTGFWIWSGKTLQSWRRFYHRLSHSSKGET


 


AV.






In another embodiment, the FZD7 protein has an AA sequence set forth in one of the following GenBank entries: AL110146 and NM—003507. In another embodiment, the FZD7 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the FZD7 protein is any other FZD7 protein known in the art. In another embodiment, the FZD7 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the FZD7 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the FZD7 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the FZD7 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the FZD7 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CLIC4 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 317; GenBank Accession No. NM_013943)







MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMILWLKGV


 


VFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLC


 


PPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQK


 


LDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKV


 


VAKKYRNFDIPKEMTGIWRYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKR


 


LTK.






In another embodiment, the CLIC4 protein has an AA sequence set forth in one of the following GenBank entries: AL080061 and NM—001830. In another embodiment, the CLIC4 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CLIC4 protein is any other CLIC4 protein known in the art. In another embodiment, the CLIC4 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CLIC4 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CLIC4 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CLIC4 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CLIC4 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating an AGA in a subject, the method comprising the step of contacting the subject or the scalp thereof with a compound or composition that decreases an activity or level of a response to biotic stimulus protein, thereby treating an AGA in a subject.
“Response to biotic stimulus protein” refers, in another embodiment, to a protein classified as such based on Gene Ontology (GO) classifications. In another embodiment, the term refers to any other definition thereof known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating an AGA in a subject, the method comprising the step of contacting the subject or the scalp thereof with a compound or composition that decreases an activity or level of a defense response protein, thereby treating an AGA in a subject.
“Defense response protein” refers, in another embodiment, to a protein classified as such based on Gene Ontology (GO) classifications. In another embodiment, the term refers to any other definition thereof known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating an AGA in a subject, the method comprising the step of contacting the subject or the scalp thereof with a compound or composition that decreases an activity or level of an immune response protein, thereby treating an AGA in a subject.
In another embodiment, the present invention provides a method of treating an AGA in a subject, the method comprising the step of contacting the subject or the scalp thereof with an anti-inflammatory medication or compound, thereby treating an AGA in a subject. In another embodiment, the medication or compound is cyclosporine. In another embodiment, the medication or compound is prednisone. In another embodiment, the medication or compound is a topical calcinuerin inhibitor. In another embodiment, the medication or compound is protopic. In another embodiment, the medication or compound is elidel. In another embodiment, the medication or compound is aspirin. In another embodiment, the medication or compound is any other anti-inflammatory medication or compound known in the art. Each possibility represents a separate embodiment of the present invention.
“Immune response protein” refers, in another embodiment, to a protein classified as such based on Gene Ontology (GO) classifications. In another embodiment, the term refers to any other definition thereof known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing a level of a protein encoded by a gene shown in the present invention to be enriched in haired scalp, thereby stimulating a hair growth in a subject. In another embodiment, the gene is depicted in FIG. 3. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing an activity of a protein encoded by a gene shown in the present invention to be enriched in haired scalp, thereby stimulating a hair growth in a subject. In another embodiment, the gene is depicted in FIG. 3. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits a prostaglandin D2 activity in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that decreases a level of a prostaglandin D2 in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits an activity of a PGD2S enzyme in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating acne in a skin of a subject.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition capable of decreasing an activity of a target gene of the present invention, thereby treating acne in a skin of a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of treating acne in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition capable of decreasing an expression of a target gene of the present invention, thereby treating acne in a skin of a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the acne that is treated by a method of present invention is androgen dependent. In another embodiment, the acne is associated with enlargement of the sebaceous glands. In another embodiment, the acne is associated with sebaceous gland hyperactivity. In another embodiment, the androgen-dependent effect results in both hair loss and sebaceous hyperplasia. In another embodiment, the androgen-dependent effect results in both hair loss and sebaceous gland hyperactivity. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits a prostaglandin D2 activity in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that decreases a level of a prostaglandin D2 in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that inhibits an activity of a PGD2S enzyme in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating rosacea in a skin of a subject.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition capable of decreasing an activity of a target gene of the present invention, thereby treating rosacea in a skin of a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition capable of decreasing an expression of a target gene of the present invention, thereby treating rosacea in a skin of a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of treating rosacea in a skin of a subject, comprising the step of contacting the subject or the skin thereof with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the skin, thereby treating rosacea in a skin of a subject.
Methods for measuring sebaceous gland hypertrophy are well known in the art, and are described, for example, in Petersen M J et al (Development of a nude mouse model to study human sebaceous gland physiology and pathophysiology. J Clin Invest. 1984 October; 74(4):1358-65) and in Benavides F et al (Impaired hair follicle morphogenesis and cycling with abnormal epidermal differentiation in nackt mice, a cathepsin L-deficient mutation. Am J Pathol. 2002 August; 161(2):693-703). Each method represents a separate embodiment of the present invention.
In another embodiment, the rosacea that is treated by a method of present invention is androgen dependent. In another embodiment, the rosacea is associated with enlargement of the sebaceous glands. In another embodiment, the rosacea is associated with sebaceous gland hyperactivity. In another embodiment, the androgen-dependent effect results in both hair loss and flushing. In another embodiment, the androgen-dependent effect results in both hair loss and sebaceous hyperplasia. In another embodiment, the androgen-dependent effect results in both hair loss and sebaceous gland hyperactivity. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating prostate cancer in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the prostate gland, thereby treating prostate cancer in a subject.
In another embodiment, the present invention provides a method of treating BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the prostate gland, thereby treating prostate BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the prostate gland, thereby treating prostate cancer in a subject.
In another embodiment, the present invention provides a method of treating BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the prostate gland, thereby treating BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer in a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the prostate gland, thereby treating prostate cancer in a subject.
In another embodiment, the present invention provides a method of treating BPH in a subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the prostate gland, thereby treating BPH in a subject.
In another embodiment, the present invention provides a method of treating prostate cancer in a subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the prostate gland, thereby treating prostate cancer in a subject.
In another embodiment, the present invention provides a method of treating BPH in a subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the prostate gland, thereby treating BPH in a subject.
In another embodiment, the prostate cancer or BPH that is treated by a method of present invention is androgen dependent. In another embodiment, the androgen-dependent effect results in prostate cancer or BPH. In another embodiment, the androgen-dependent effect results in prostate cancer. In another embodiment, the androgen-dependent effect results in BPH. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that inhibits a prostaglandin D2 activity in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that inhibits a signaling or receptor pathway downstream of a prostaglandin D2 in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that inhibits an activity of a PGD2S enzyme in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition capable of decreasing an activity of a target gene of the present invention, thereby suppressing fertility of a male subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition capable of decreasing an expression of a target gene of the present invention, thereby suppressing fertility of a male subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of suppressing fertility of a male subject, comprising the step of contacting the subject with a compound or composition that decreases a level of a prostaglandin D2 synthase enzyme in the testes, thereby suppressing fertility of a male subject.
In another embodiment, the fertility that is suppressed by a method of present invention is dependent on expression of PGD2 by Leydig cells. In another embodiment, the expression of PGD2 is androgen dependent. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of decreasing an activity of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of decreasing an expression of a target gene of the present invention, thereby stimulating a hair growth in a subject. In another embodiment, the gene is selected from CCL18, Col11A1, Col3A1, CD4, Cd1a, FCER1A, HLA-C, and HLA-DPA1. In another embodiment, the gene is selected from IGF1, GPR105, PDGFRL, ADRA2A, CCL19, and CORIN. Each possibility represents another embodiment of the present invention.
In another embodiment, the target gene is CCL18. In another embodiment, the target gene is Col11A1. In another embodiment, the target gene is Col3A1. In another embodiment, the target gene is CD4. In another embodiment, the target gene is Cd1a. In another embodiment, the target gene is FCER1A. In another embodiment, the target gene is HLA-C. In another embodiment, the target gene is HLA-DPA1. In another embodiment, the target gene is IGF1. In another embodiment, the target gene is GPR105. In another embodiment, the target gene is PDGFRL. In another embodiment, the target gene is ADRA2A. In another embodiment, the target gene is CCL19. In another embodiment, the target gene is PTGD2. In another embodiment, the target gene is CORIN. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a compound or composition utilized in a method or composition of the present invention negatively regulates expression of the gene encoding the target protein. In another embodiment, the compound or composition negatively regulates transcription of the gene encoding the target protein. In another embodiment, the compound or composition negatively regulates translation of an RNA molecule encoding the target protein. In another embodiment, the compound or composition negatively regulates expression of the target protein in a post-translational fashion. Each possibility represents a separate embodiment of the present invention.
As provided herein, findings of the present invention demonstrate that various target genes (e.g. CCL18, Col11A1, Col13A1, CD4, Cd1a, FCER1A, HLA-C, HLA-DPA1, IGF1, GPR105, PDGFRL, ADRA2A, CCL19, PTGD2, and CORIN) are up-regulated in bald scalp. Thus, decreasing expression or activity of products of these genes in bald scalp will stimulate hair growth. In another embodiment, the target gene is CCL18. In another embodiment, the target gene is Col11A1. In another embodiment, the target gene is Col3A1. In another embodiment, the target gene is CD4. In another embodiment, the target gene is Cd1a. In another embodiment, the target gene is FCER1A. In another embodiment, the target gene is HLA-C. In another embodiment, the target gene is HLA-DPA1. In another embodiment, the target gene is any of the genes shown in FIG. 3 to be enriched in bald scalp. Each possibility represents a separate embodiment of the present invention.
The CCL18 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 259)







MKGLAAALLVLVCTMALCSCAQVGTNKELCCLVYTSWQIPQKFIVDYSET


 


SPQCPKPGVILLTKRGRQICADPNKKWVQKYISDLKLNA.






In another embodiment, the CCL18 protein has an AA sequence set forth in one of the following GenBank entries: NM—002988, Y13710, BC069700, BC096124, BC096125, BC096126, BC096127, AB000221, and CR407660. In another embodiment, the CCL18 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CDL18 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDL18 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDL18 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDL18 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CDL18 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The Col11A1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 260)









MFSFVDLRLLLLLAATALLTHGQEEGQVEGQDEDIPPITCVQNGLRYHDRDVWKPEPCRICV



 


CDNGKVLCDDVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPR


 


GPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGGISVPGPMGPSGP


 


RGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPP


 


GPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLP


 


GERGRPGAPGPAGARGNDGATGAAGPPGPTGPAGPPGFPGAVGAKGEAGPQGPRGSEGPQ


 


GVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPGGPP


 


GPKGNSGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGLPGPPGERG


 


GPGSRGFPGADGVAGPKGPAGERGSPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGP


 


DGKTGPPGPAGQDGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGP


 


AGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGA


 


PGPSGARGERGFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQG


 


MPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPPGPAGAPGDKGESGPSG


 


PAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAG


 


PPGPIGNVGAPGAKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGGKGP


 


RGETGPAGRPGEVGPPGPPGPAGEKGSPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGER


 


GFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGA


 


KGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPVGPVGARGPAGPQGP


 


RGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPGKD


 


GLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYY


 


RADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWID


 


PNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGESMTDGFQFE


 


YGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIR


 


AEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLRIIDVAPLDVGAPDQEFGFDVGHV


 


CFL.






In another embodiment, the Col11A1 protein has an AA sequence set forth in one of the following GenBank entries: BC036531, M32798, NM—000088, K01228, M32798, J00110, J00111, J00112, J00113, M36546, 864596, BC036531, AB209597, X06269, X07884, and Z74615. In another embodiment, the Col11A1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the Col11A1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col11A1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col11A1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col11A1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col11A1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention Each possibility represents a separate embodiment of the present invention.
The Col3A1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 261)









MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVWKPEPCQICVCDSGSVL



 


CDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGPQGPKGDPGPPGIPGRNGDPGI


 


PGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGT


 


SGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGI


 


KGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGR


 


PGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEP


 


GPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGA


 


GEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFR


 


GPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKP


 


GPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKN


 


GETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPG


 


GKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERG


 


GLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGP


 


RGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGKDGTSGHP


 


GPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPY


 


YGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDP


 


NQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYG


 


NPELPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKA


 


EGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVC


 


FL.






In another embodiment, the Col3A1 protein has an AA sequence set forth in one of the following GenBank entries: BC028178, AK091853, NM—000090, M13146, M11134, S79877, M59227, BC028178, X01655, X01742, X07240, X14420, and X15332. In another embodiment, the Col3A1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the Col3A2 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col3A2 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col3A2 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col3A2 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the Col3A2 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CD4 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 262)







MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQKKSIQ


 


FHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLK


 


IEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSS


 


PSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIV


 


VLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKS


 


WITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLA


 


LEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAK


 


VSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMALI


 


VLGGVAGLLLFIGLGIFFCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHR


 


FQKTCSPI.






In another embodiment, the CD4 protein has an AA sequence set forth in one of the following GenBank entries: NM—000616, DQ012936, BT019811, BT019791, DQ012936, AAY22175, M35160, AAA16069, U47924, AAB51309, X87579, CAA60883, BC025782, AAH25782, BT019791, AAV38594, BT019811, AAV38614, DA463597, DB123455, M12807, AAA35572, S79267, AAB35273, U40625, AAB02789. In another embodiment, the CD4 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CD4 protein is encoded by a gene at the location 12pter-p12. In another embodiment, the CD4 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD4 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD4 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD4 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD4 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The Cd1a protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 263)







ADGLKEPLSFHVTWIASFYNHSWKQNLVSGWLSDLQTHTWDSNSSTIVFL


 


CPWSRGNFSNEEWKELETLFRIRTIRSFEGIRRYAHELQFE.






In another embodiment, the Cd1a protein has an AA sequence set forth in one of the following GenBank entries: AF142665, AAD37578, AL121986, CAI10848, M14663, AAA51934, M22167, AAA51932, BC031645, AAH31645, CR603267, M27735, AAA51933, M28825, AAA51931, X04450, and CAA28049. In another embodiment, the Cd1a protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention. In another embodiment, the Cd1a protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the Cd1a protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the Cd1a protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the Cd1a protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the Cd1a protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The FCER1A protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 264)







MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLT


 


CNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNE


 


SEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGE


 


ALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPRE


 


KYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHP


 


KPNPKNN.






In another embodiment, the FCER1A protein has an AA sequence set forth in one of the following GenBank entries: A21606, CAA01564, AB059236, BAB84120, L14075, AAA16115, BC005912, AAH05912, BC015185, BC015195, AAH15195, J03605, AAA36204, X06948, and CAA30025. In another embodiment, the FCER1A protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the FCER1A protein has an AA or nucleotide sequence set forth in a GenBank entry for a FCER1A protein. In another embodiment, the FCER1A protein has an AA or nucleotide sequence set forth in a GenBank entry for a FcERI protein. Each possibility represents a separate embodiment of the present invention. In another embodiment, the FCER1A protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the FCER1A protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the FCER1A protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the FCER1A protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the FCER1A protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The HLA-C protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 265)







SHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPW


 


VEQEGPEYWDRETQKYKRQAQADRVNLRKLRGYYNQSEDGSHTLQWMYGC


 


DLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAR


 


EAEQLRAYLEGTCVEWLRRYLENGKETLQRA.






In another embodiment, the HLA-C protein has an AA sequence set forth in one of the following GenBank entries:

    AB008136, BAA22919, AB032096, BAA84115, AB088111, BAC54942, AB196344, BAD77815, AB196345, BAD77816, AB196346, BAD77817, AB202106, BAE78631, AB247153, BAE76021, AB247154, BAE76022, AF002270, AAC14581, AF002271, AAB62539, AF003284, AAC17716, AF009682, AAB66708, AF009684, AAB66710, AF009685, AAB66711, AF015557, AAC17722, AF015559, AAC17724, AF016304, AAB80724, AF017322, AAB70288, AF017323, AAB70289, AF017324, AAB70290, AF017325, AAB70291, AF017326, AAB70292, AF017329, AAB70295, AF017330, AAB70296, AF017331, AAC14579, AF019568, AAC17721, AF036551, AAC17727, AF036553, AAC17725, AF036555, AAC17713, AF036557, AAC17720, AF037075, AAC17717, AF037077, AAC17718, AF037079, AAC17715, AF037450, AAC17710, AF038574, AAC17723, AF039198, AAD37816, AF062587, AAC16245, AF062588, AAC16245, AF069961, AAF23073, AF076476, AAC27626, AF082801, AAD52014, AF100320, AAF04638, AF102689, AAF04743, AF105241, AAF29567, AF138277, AAF64391, AF139728, AAF65514, AF144665, AAD38379, AF145467, AAD38672, AF145761, AAD38673, AF145763, AAD38674, AF147702, AAD53513, AF165851, AAF89551, AF165853, AAF89554, AF172868, AAD48066, AF176082, AAD51747, AF179632, AAD52687, AF189726, AAF04581, AF205537, AAF19427, AF220291, AAF33239, AF245437, AAG53741, AF250557, AAG53742, AF281056, AAK69510, AF284583, AAF91475, AF289031, AAG10048, AF316036, AAG49322, AF323844, AAG40881, AF323846, AAG40882, AF323854, AAG42272, AF335315, AAK07655, AF480614, AAL87225, AF497547, AAM46846, AF509727, AAP30863, AF510721, AAM44831, AF525406, AAM83098, AF525408, AAM91947, AF529191, AAN04004, AF535212, AAN07187, AF539619, AAN33107, AF541997, AAN10165, AF541998, AAN10166, AJ001977, CAA05125, AJ010322, CAB45917, AJ010323, CAB45918, AJ011881, CAB65545, AJ011882, CAB65546, AJ011883, CAB65547, AJ011884, CAB65548, AJ131194, CAC11132, AJ133100, CAB38942, AJ133473, CAB37942, AJ133475, CAB37944, AJ133476, CAB37945, AJ237703, CAB40352, AJ238694, CAB41889, AJ242661, CAB44356, AJ243434, CAB46485, AJ249163, CAB53538, AJ249164, CAB53539, AJ277100, CAB86064, AJ278494, CAB95011, AJ278509, CAC01936, AJ291815, CAC19191, AJ292559, CAC05372, AJ293016, CAC04321, AJ293017, CAC04322, AJ293511, CAB97130, AJ298116, CAC18746, AJ298837, CAC12745, AJ300765, CAC24483, AJ304496, CAC19018, AJ306617, CAC36100, AJ318865, CAC79613, AJ418708, CAD12801, AJ420242, CAD12427, AJ420243, CAD12428, AJ420245, CAD12430, AJ420247, CAD12432, AJ420248, CAD12433, AJ420251, CAD12436, AJ420252, CAD12437, AJ438882, CAD27616, AJ440717, CAD29450, AJ440718, CAD29451, AJ506097, CAD44569, AJ506196, CAD44641, AJ507389, CAD45440, AJ507431, CAD45557, AJ507797, CAD47827, AJ507798, CAD47856, AJ515217, CAD56468, AJ535690, CAD59685, AJ549184, CAD70710, AJ549185, CAD70711, AJ550622, CAD79471, AJ550623, CAD79472, AJ558126, CAD90006, AJ558127, CAD90007, AJ558128, CAD90008, AJ558129, CAD90009, AJ558130, CAD90010, AJ558132, CAD90012, AJ566950, CAD98751, AJ579644, CAE22463, AJ579645, CAE22464, AJ579646, CAE22465, AJ579787, CAE30287, AJ579997, CAE30455, AJ579998, CAE30456, AJ580001, CAE30459, AJ580002, CAE30460, AJ616764, CAE84097, AJ616766, CAE84099, AJ616776, CAE84103, AJ616772, CAE84105, AJ616774, CAE84107, AJ617511, CAE85468, AJ617512, CAE85469, AJ617783, CAE92337, AJ617785, CAE92338, AJ620461, CAF05030, AJ621024, CAF18233, AJ628733, CAF32225, AJ628735, CAF32226, AJ628737, CAF32227, AJ628741, CAF32229, AJ629313, CAF33342, AJ629315, CAF33343, AJ635295, CAG25672, AJ635299, CAG25674, AJ697649, CAG26752, AJ697650, CAG26753, AJ697852, CAG26974, AJ810176, CAH17687, AJ831405, CAH40829, AJ851863, CAH65480, AJ851864, CAH65479, AJ851865, CAH65478, AJ865388, CAI29271, AJ871635, CAI40345, AJ879943, CAI53896, AJ971029, CAI94871, AL662833, CAI17409, AL671883, CAI18144, AM039493, CAJ01384, AM087019, CAJ31318, AM087957, CAJ32664, AM087958, CAJ32665, AM157129, CAJ42902, AM180629, CAJ55687, AM180647, CAJ55680, AM180648, CAJ55681, AM180649, CAJ55682, AM180651, CAJ55690, AM180722, CAJ55741, AM180941, CAJ56101, AY028706, AAK31618, AY028708, AAK31619, AY064404, AAL49978, AY078079, AAL82717, AY093610, AAM14725, AY158888, AAO49822, AY162385, AAN84536, AY181042, AAO24136, AY217669, AAO62346, AY229982, AAO74627, AY230857, AAO73564, AY233979, AAO84019, AY323834, AAO84346, AY354908, AAR28201, AY363885, AAR19086, AY363887, AAR19102, AY364410, AAR15062, AY368486, AAR28679, AY368504, AAQ72735, AY371078, AAR15147, AY371080, AAR15148, AY373452, AAQ81571, AY429571, AAR06856, AY429604, AAR08453, AY429725, AAR10880, AY429727, AAR10881, AY434500, AAR12134, AY438570, AAR06668, AY438572, AAR06669, AY484703, AAR87009, AY509614, AAR99590, AY509615, AAR99591, AY509618, AAR99594, AY530953, AAS98883, AY536515, AAS45436, AY607028, AAT46360, AY618875, AAT39426, AY619989, AAT41622, AY623606, AAT39989, AY643837, AAT65966, AY714373, AAU14145, AY839182, AAW21339, AY887667, AAW88385, AY918170, AAX18631, AY918171, AAX18632, AY929155, AAX98239, AY973960, AAX94769, AY973961, AAX94770, BA000025, BAB63310, D64153, BAA11022, D83957, BAA22206, D89334, BAA13946, DQ003053, AAY23010, DQ020591, AAY46194, DQ086795, AAZ28915, DQ086799, AAZ28917, DQ135946, AAZ53371, DQ135948, AAZ53372, DQ244128, ABB52621, DQ244130, ABB52622, DQ244132, ABB52623, DQ244134, ABB52624, DQ270211, ABB55457, DQ289052, ABC02064, DQ289054, ABC02065, DQ327710, ABC59292, DQ334740, ABC61964, DQ334744, ABC61966, DQ359691, ABD60574, DQ400515, ABD62869, DQ400525, ABD62874, DQ400527, ABD62875, DQ400531, ABD62877, L54059, AAB04639, M16272, AAA59700, M16273, AAA59702, M24030, AAA59671, M26432, AAA59648, M28172, AAA59670, M59865, AAA59659, U31373, AAA74583, U38975, AAA81370, U38976, AAA81371, U44064, AAB02773, U52176, AAB82334, U56260, AAB01210, U60217, AAB03578, U60218, AAB03579, U60321, AAB03581, U60322, AAB03582, U60323, AAB03583, U60324, AAB03584, U60422, AAB03587, U61273, AAB03623, U61274, AAB03624, U80671, AAB38971, U81012, AAB40998, U88251, AAB48495, U88252, AAB48496, U88253, AAB48497, U88839, AAB48516, U96785, AAC17737, U96787, AAC17719, U96789, AAC17728, U97345, AAC17714, U97347, AAC17711, X00495, CAA25190, Y14684, CAA75000, Y15745, CAA75755, Y15746, CAA75756, Y15842, CAB44777, Y16411, CAA76197, Y16412, CAA76198, Y16418, CAA76206, Y17064, CAA76613, Y17065, CAA76614, Y18139, CAB71932, Y18141, CAB71934, Y18142, CAB71935, Y18143, CAB71936, Y18145, CAB71938, Y18146, CAB71939, Y18499, CAB71315, Y18656, CAB71940, Z22752, CAA80437, Z72007, CAA96520, Z75172, CAA99487, Z79751, CAB02077, Z80228, CAB02409, AF196489, AAM76870, AF405691, AAL03994, AJ005199, CAA06438, AJ010749, CAA09341, AJ440716, CAD29433, AJ537578, CAD61205, AK024836, AK130592, AY732487, AAV33126, BC002463, AAH02463, BC004489, BC007814, AAH07814, BC008457, AAH08457, BC010542, AAH10542, BC033293, AAH33293, BC041078, CA395932, CR590624, CR591580, CR592947, CR593350, CR595198, CR596955, CR597526, CR598290, CR598918, CR599470, CR600524, CR601147, CR602127, CR602442, CR603910, CR604220, CR605658, CR606564, CR606802, CR606838, CR607146, CR607446, CR607744, CR608009, CR608133, CR608445, CR608734, CR610206, CR610282, CR610894, CR611167, CR611253, CR611808, CR612894, CR613417, CR617532, CR619374, CR619491, CR619732, CR620918, CR622501, CR623932, CR625377, CR626083, CR626476, CR626681, D38526, BAA07531, D49552, BAA08500, D49819, BAA08625, D50852, BAA32611, D50853, BAA32610, D50854, BAA32612, D64145, BAA19531, D64146, BAA19532, D64147, BAA19533, D64150, BAA19535, D64151, BAA19536, D64152, BAA19537, D83029, BAA11747, D83030, BAA32613, D83741, BAA32615, L38251, AAF26750, L77114, AAL40067, M11886, AAA52665, M12679, AAA59699, M21963, AAA59847, M24031, AAA00027, M24096, AAA59654, M24097, AAA59656, AAA59657, M24097, AAA59656, AAA59657, M26429, AAA59701, M26430, AAA59703, M26431, AAA59704, M26712, AAA52666, M28160, AAA36235, M28206, AAA57258, M28207, AAA53259, M84171, AAA59685, M84172, AAA59686, M84386, AAA59705, M99389, AAA88088, M99390, AAA88089, S74115, AAD14147, U06695, AAA92995, U06696, AAA92996, U07230, AAA17037, U09853, AAA50217, U41386, AAC32743, U41420, AAC32742, U62824, AAB67322, X58536, CAA41427, X70856, CAA50209, X70857, CAA50210, X76189, CAA53783, X82122, CAA57634, X83394, CAA58313, X97321, CAA65986, X98742, CAA67294, X99704, CAA68035, Y10520, CAA71531, Y11843, CAA72592, Z33459, CAA83881, Z46809, CAA86839, Z46810, CAA86840, Z83247, and CAB05845. In another embodiment, the HLA-C protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-C protein has an AA or nucleotide sequence set forth in a GenBank entry for a Cw*1701 protein. In another embodiment, the HLA-C protein has an AA or nucleotide sequence set forth in a GenBank entry for a D6S204 protein. In another embodiment, the HLA-C protein has an AA or nucleotide sequence set forth in a GenBank entry for a FLJ27082 protein. In another embodiment, the HLA-C protein has an AA or nucleotide sequence set forth in a GenBank entry for a HLA-JY3 protein. In another embodiment, the HLA-C protein has an AA or nucleotide sequence set forth in a GenBank entry for a HLC-C protein. In another embodiment, the HLA-C protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-C protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-C protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-C protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-C protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.


The HLA-DPA1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 266)







TFCRVFLYFLYAADHVSTYAMFVQTHRPTGEFMFEFDEDEMFYVDLDKKE


 


TVWHLEEFGQAFSFEAQGGLANIAILNNNLNTLIQRSNHTQATNGTPYLC


 


LFLCSPTG.






In another embodiment, the HLA-DPA1 protein has an AA sequence set forth in one of the following GenBank entries: AF013767, AAC64233, AF015295, AAC61669, AF074847, AAD39684, AF074848, AAD39685, AF076284, AAD42927, AF076285, AAD42928, AF092049, AAF00051, AF098794, AAC72845, AF165160, AAD47826, AF346471, AAK27152, AL645931, CAI18040, CAI18041, AL662824, CAI17598, CAI17599, CAI17600, AL805913, CAI18549, AY335522, AAR01827, AY335523, AAR01829, AY335524, AAR01831, AY335525, AAR01833, AY335526, AAR01835, AY335527, AAR01837, AY335528, AAR01839, AY335529, AAR01841, AY335530, AAR01843, AY335531, AAR01845, AY335532, AAR01847, AY335533, AAR01849, AY335534, AAR01851, AY335535, AAR01853, AY335536, AAR01855, AY335537, AAR01857, AY335538, AAR01859, AY335539, AAR01861, AY335540, AAR01863, AY335541, AAR01865, AY335542, AAR01867, AY335543, AAR01869, AY335544, AAR01871, AY375760, AAQ84179, AY375761, AAQ84180, AY375762, AAQ84181, AY375763, AAQ84182, AY375764, AAQ84183, AY375765, AAQ84184, AY375766, AAQ84185, AY375767, AAQ84186, AY375768, AAQ84187, AY375769, AAQ84188, AY375770, AAQ84190, AY375771, AAQ84189, AY375775, AAQ84191, AY375780, AAQ84192, AY375781, AAQ84193, AY375787, AAQ84194, AY375791, AAQ84195, AY375827, AAQ84196, AY375828, AAQ84197, AY375829, AAQ84198, AY375830, AAQ84199, AY375831, AAQ84200, AY375832, AAQ84201, AY375833, AAQ84202, AY375834, AAQ84203, AY375835, AAQ84204, AY375836, AAQ84205, AY375837, AAQ84206, AY375838, AAQ84207, AY375839, AAQ84208, AY375840, AAQ84209, AY618552, AAT92096, AY618553, AAT92097, AY650051, AAT67468, DQ274060, ABB88406, DQ274061, ABB88407, K00514, AAA59786, M23903, M23904, U87556, AAB97110, X02228, CAA26148, X03100, CAA26887, X78199, X82393, X82394, BC009956, AAH09956, CR601798, CR617728, M27487, AAA63220, X00457, and CAA25143. In another embodiment, the HLA-DPA1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-DPA1 protein has an AA or nucleotide sequence set forth in a GenBank entry for a HLA-DPA1A protein. In another embodiment, the HLA-DPA1 protein has an AA or nucleotide sequence set forth in a GenBank entry for a HLADP protein. In another embodiment, the HLA-DPA1 protein has an AA or nucleotide sequence set forth in a GenBank entry for a HLASB protein. In another embodiment, the HLA-DPA1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-DPA1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-DPA1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-DPA1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the HLA-DPA1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The IGF1 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 318; GenBank Accession No: NM_000618)







MGKISSLPTQLFKCCFCDFLKVKMHTMSSSHLFYLALCLLTFTSSATAGP


 


ETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSC


 


DLRRLEMYCAPLKPAKSARSVRAQRHTDMPKTQKEVHLKNASRGSAGNKN


 


YRM.






In another embodiment, the IGF1 protein has an AA sequence set forth in one of the following GenBank entries: X57025 and M11568. In another embodiment, the IGF1 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the IGF1 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the IGF1 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the IGF1 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the IGF1 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the IGF1 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The GPR105 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 319; GenBank Accession No: BC034989)







MINSTSTQPPDESCSQNLLITQQIIPVLYCMVFIAGILLNGVSGWIFFYV


 


PSSESFIIYLKNIVIADFVMSLTFPFKILGDSGLGPWQLNVFVCRVSAVL


 


FYVNMYVSIVFFGLISFDRYYKIVKPLWTSFIQSVSYSKLLSVIVWMLML


 


LLAVPNIILTNQSVREVTQIKCIELKSELGRKWHKASNYIFVAIFWIVFL


 


LLIVFYTAITKKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYH


 


IARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQ


 


PFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL.






In another embodiment, the GPR105 protein has an AA sequence set forth in one of the following GenBank entries: NM—014879 and D13626. In another embodiment, the GPR105 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the GPR105 protein is referred to as “purinergic receptor P2Y, G-protein coupled, 14.” In another embodiment, the GPR105 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR105 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR105 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR105 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the GPR105 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The PDGFRL (Platelet-derived growth factor receptor-like) protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 320; GenBank Accession No: BC010927)







MKVWLLLGLLLVHEALEDVTGQHLPKNKRPKEPGENRIKPTNKKVKPKIP


 


KMKDRDSANSAPKTQSIMMQVLDKGRFQKPAATLSLLAGQTVELRCKGSR


 


IGWSYPAYLDTFKDSRLSVKQNERYGQLTLVNSTSADTGEFSCWVQLCSG


 


YICRKDEAKTGSTYIFFTEKGELFVPSPSYFDVVYLNPDRQAVVPCRVTV


 


LSAKVTLHREFPAKEIPANGTDIVYDMKRGFVYLQPHSEHQGVVYCRAEA


 


GGRSQISVKYQLLYVAVPSGPPSTTILASSNKVKSGDDISVLCTVLGEPD


 


VEVEFTWIFPGQKDERPVTIQDTWRLIHRGLGHTTRISQSVITVEDFETI


 


DAGYYICTAQNLQGQTTVATTVEFS.






In another embodiment, the PDGFRL protein has an AA sequence set forth in one of the following GenBank entries: D37965 and NM—006207. In another embodiment, the PDGFRL protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the PDGFRL protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the PDGFRL protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the PDGFRL protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the PDGFRL protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the PDGFRL protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The ADRA2A protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 321; GenBank Accession No: BC050414)







MGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFG


 


NVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWY


 


FGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRI


 


KAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISS


 


CIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRP


 


NGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEESSSSD


 


HAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTPAAGPG


 


EERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVG


 


CSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRI


 


V.






In another embodiment, the ADRA2A protein has an AA sequence set forth in one of the following GenBank entries: AF284095 and NM—000681. In another embodiment, the ADRA2A protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the ADRA2A protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the ADRA2A protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the ADRA2A protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the ADRA2A protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the ADRA2A protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CCL19 protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:







(SEQ ID No: 322; GenBank Accession No: BC027968)







MALLLALSLLVLWTSPAPTLSGTNDAEDCCLSVTQKPIPGYIVRNFHYLL


 


IKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS.






In another embodiment, the CCL19 protein has an AA sequence set forth in one of the following GenBank entries: AB000887 and NM—006274. In another embodiment, the CCL19 protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CCL19 protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CCL19 protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CCL19 protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CCL19 protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CCL19 protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The CORIN protein targeted by methods and compositions of the present invention has, in another embodiment, the sequence:








(SEQ ID No: 323; GenBank Accession No: AF133845)









MKQSPALAPEERYRRAGSPKPVLRADDNNMGNGCSQKLATANLLRFLLLVLIPCICALVLLLVILL



 


SYVGTLQKVYFKSNGSEPLVTDGEIQGSDVILTNTIYNQSTVVSTAHPDQHVPAWTTDASLPGDQS


 


HRNTSACMNITHSQCQMLPYHATLTPLLSVVRNMEMEKFLKFFTYLHRLSCYQHIMLFGCTLAFP


 


ECIIDGDDSHGLLPCRSFCEAAKEGCESVLGMVNYSWPDFLRCSQFRNQTESSNVSRICFSPQQEN


 


GKQLLCGRGENFLCASGICIPGKLQCNGYNDCDDWSDEAHCNCSENLFHCHTGKCLNYSLVCDG


 


YDDCGDLSDEQNCDCNPTTEHRCGDGRCIAMEWVCDGDHDCVDKSDEVNCSCHSQGLVECRN


 


GQCIPSTFQCDGDEDCKDGSDEENCSVIQTSCQEGDQRCLYNPCLDSCGGSSLCDPNNSLNNCSQC


 


EPITLELCMNLPYNSTSYPNYFGHRTQKEASISWESSLFPALVQTNCYKYLMFFSCTILVPKCDVNT


 


GERIPPCRALCEHSKERCESVLGIVGLQWPEDTDCSQFPEENSDNQTCLMPDEYVEECSPSHFKCR


 


SGQCVLASRRCDGQADCDDDSDEENCGCKERDLWECPSNKQCLKHTVICDGFPDCPDYMDEKN


 


CSFCQDDELECANHACVSRDLWCDGEADCSDSSDEWDCVTLSINVNSSSFLMVHRAATEHHVCA


 


DGWQEILSQLACKQMGLGEPSVTKLIQEQEKEPRWLTLHSNWESLNGTTLHELLVNGQSCESRSK


 


ISLLCTKQDCGRRPAARMNKRILGGRTSRPGRWPWQCSLQSEPSGHICGCVLIAKKWVLTVAHCF


 


EGRENAAVWKVVLGINNLDHPSVFMQTRFVKTIILHPRYSRAVVDYDISIVELSEDISETGYVRPV


 


CLPNPEQWLEPDTYCYITGWGHMGNKMPFKLQEGEVRIISLEHCQSYFDMKTITTRMICAGYESG


 


TVDSCMGDSGGPLVCEKPGGRWTLFGLTSWGSVCFSKVLGPGVYSNVSYFVEWIKRQIYIQTFLL


 


N.






In another embodiment, the CORIN protein has an AA sequence set forth in one of the following GenBank entries: NM—006587, AF133845, and BC110451. In another embodiment, the CORIN protein is encoded by a gene having a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CORIN protein is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CORIN protein is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CORIN protein is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CORIN protein is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CORIN protein comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of decreasing a level of a protein encoded by a gene shown in the present invention to be enriched in bald scalp, thereby stimulating a hair growth in a subject. In another embodiment, the gene is depicted in FIG. 3. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of decreasing an activity of a protein encoded by a gene shown in the present invention to be enriched in bald scalp, thereby stimulating a hair growth in a subject. In another embodiment, the gene is depicted in FIG. 3. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a scalp in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of decreasing an inflammation in the scalp, thereby stimulating a hair growth in a scalp in a subject. As provided herein, results of the present invention demonstrate that proteins that mediate inflammation are enriched in bald scalp.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a prostaglandin D2 (PGD2) in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 metabolite in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing a subject for a presence of an AGA, comprising the steps of: (a) measuring a level of a PGD2 metabolite in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has AGA.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in the scalp of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in the scalp of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in a body fluid of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of baldness therapy in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in a body fluid of the subject, and (b) administering the baldness therapy to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the baldness therapy is efficacious.
In another embodiment, the present invention provides a method of testing a subject for a presence of acne, comprising the steps of: (a) measuring a level of a PGD2 in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has acne.
In another embodiment, the present invention provides a method of testing a subject for a presence of acne, comprising the steps of: (a) measuring a level of a PGD2 metabolite in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has acne.
In another embodiment, the present invention provides a method of testing a subject for a presence of acne, comprising the steps of: (a) measuring a level of a PGD2 in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has acne.
In another embodiment, the present invention provides a method of testing a subject for a presence of acne, comprising the steps of: (a) measuring a level of a PGD2 metabolite in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has acne.
In another embodiment, the present invention provides a method of testing a subject for a presence of rosacea, comprising the steps of: (a) measuring a level of a PGD2 in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has rosacea.
In another embodiment, the present invention provides a method of testing a subject for a presence of rosacea, comprising the steps of: (a) measuring a level of a PGD2 metabolite in the scalp of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has rosacea.
In another embodiment, the present invention provides a method of testing a subject for a presence of rosacea, comprising the steps of: (a) measuring a level of a PGD2 in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has rosacea.
In another embodiment, the present invention provides a method of testing a subject for a presence of rosacea, comprising the steps of: (a) measuring a level of a PGD2 metabolite in a body fluid of the subject; and (b) comparing the level to a reference standard, wherein, if the level is appreciably elevated relative to the reference standard, then the subject has rosacea.
In another embodiment, the present invention provides a method of testing an efficacy of therapy for acne or rosacea in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in the scalp of the subject, and (b) administering the therapy for acne or rosacea to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the therapy for acne or rosacea is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of therapy for acne or rosacea in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in the scalp of the subject, and (b) administering the therapy for acne or rosacea to the subject; and (c) measuring a second level of the PGD2 in the scalp, wherein, if the second level is significantly decreased relative to the first level, then the therapy for acne or rosacea is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of therapy for acne or rosacea in a subject, comprising the steps of: (a) measuring a first level of a PGD2 in a body fluid of the subject, and (b) administering the therapy for acne or rosacea to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the therapy for acne or rosacea is efficacious.
In another embodiment, the present invention provides a method of testing an efficacy of therapy for acne or rosacea in a subject, comprising the steps of: (a) measuring a first level of a PGD2 metabolite in a body fluid of the subject, and (b) administering the therapy for acne or rosacea to the subject; and (c) measuring a second level of the PGD2 in the body fluid, wherein, if the second level is significantly decreased relative to the first level, then the therapy for acne or rosacea is efficacious.
“Appreciably” and “significantly” refer, in another embodiment, to a change of at least 1 standard deviation (std). In another embodiment, the terms refer to a change of at least 1.5 std. In another embodiment, the terms refer to a change of at least 2 std. In another embodiment, the terms refer to a change of at least 3 std. In another embodiment, the terms refer to a change of at least 4 std. In another embodiment, the terms refer to a change of more than 1.5 std. In another embodiment, the terms refer to a change of more than 2 std. In another embodiment, the terms refer to a change of more than 3 std. In another embodiment, the terms refer to a change of more than 4 std.
In another embodiment, the terms refer to a change of at least 50%. In another embodiment, the terms refer to a change of at least 70%. In another embodiment, the terms refer to a change of at least 2 fold (e.g. a doubling or a reduction by 50%). In another embodiment, the terms refer to a change of at least 3 fold. In another embodiment, the terms refer to a change of at least 4 fold. In another embodiment, the terms refer to a change of at least 5 fold. In another embodiment, the terms refer to a change of more than 50%. In another embodiment, the terms refer to a change of more than 70%. In another embodiment, the terms refer to a change of more than 2 fold. In another embodiment, the terms refer to a change of more than 3 fold. In another embodiment, the terms refer to a change of more than 4 fold. In another embodiment, the terms refer to a change of more than 5 fold.
In another embodiment, the terms refer to a change considered statistically significant, relative to the reference standard, using an assay known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or level of a protein encoded by an organogenesis gene, thereby inhibiting or reversing hair loss.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or level of a protein encoded by a UV-responsive gene, thereby inhibiting or reversing hair loss. In another embodiment, the gene is selected from COL11A1, COL3A1, PCNA, CDK4, and CYCLIN G. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that decreases an activity or expression of an androgen pathway gene, thereby inhibiting or reversing hair loss. In another embodiment, the gene is selected from AR, 3beta-HSD, 17beta-HSD, 5-alpha reductase 1, and 5-alpha reductase 2. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that decreases an activity or expression of a Major Histocompatibility Complex (MHC) gene, thereby inhibiting or reversing hair loss. In another embodiment, the gene is selected from HLA-C and HLA-DPA1. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or expression leucine rich repeat-containing protein, thereby inhibiting or reversing hair loss. In another embodiment, the gene is selected from LRRC15 and GPR49. In another embodiment, the compound is a G protein small molecule activator of GPR49. In another embodiment, the compound is a G protein small molecule activator of a leucine-rich repeat-containing GPCR. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or expression of an immune-related gene, thereby inhibiting or reversing hair loss, thereby inhibiting or reversing hair loss.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or expression of an anti-protease, thereby inhibiting or reversing hair loss, thereby inhibiting or reversing hair loss. In another embodiment, the anti-protease is related to Serpin A. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or expression of an anti-vascular factor, thereby inhibiting or reversing hair loss, thereby inhibiting or reversing hair loss. In another embodiment, the anti-vascular factor is related to CDT6. In another embodiment, the anti-vascular factor suppresses angiogenesis in the high-metabolism milieu of HF. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that decreases an activity or expression of a pro-inflammatory gene, thereby inhibiting or reversing hair loss.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition that increases an activity or expression of an anti-inflammatory gene, thereby inhibiting or reversing hair loss.
In another embodiment, the present invention provides a method of inhibiting or reversing hair growth, comprising the step of stimulating a PGD2-related pathway of the present invention.
In another embodiment, the utility of methods and compositions of the present invention is not limited to AGA; rather, the methods and compositions are efficacious in any type of alopecia. The present invention has identified pathways using the normal biology of hair cycling as opposed to a pathophysiological model.
In another embodiment, the present invention provides a method of inhibiting or reversing hair loss, comprising the step of contacting a subject with a compound or composition capable of inhibiting an activity of PGD2S. In another embodiment, the activity is a lipophilic ligand-binding activity. In another embodiment, the activity is a carrier activity for retinaldehyde. In another embodiment, the activity is a carrier activity for RA. In another embodiment, the activity is a carrier activity for biliverdin. In another embodiment, the activity is a carrier activity for bilirubin. In another embodiment, the activity is a carrier activity for any other PGD2S ligand known in the art. In another embodiment, the activity is any other PGD2S activity known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a composition or method of the present invention is utilized on human skin. In another embodiment, the composition or method is utilized on an area of unwanted hair growth. In another embodiment, the area is the face. In another embodiment, the area is the bikini area. In another embodiment, the area is the legs. In another embodiment, the area is the arms. In another embodiment, the area is the chest. In another embodiment, the area is the armpits. In another embodiment, the area is any other area with undesired hair growth. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, the method comprising the steps of (a) isolating an alpha-6 integrinhigh CD200high cell population from a haired area of the subject; and (b) contacting a bald or hair-deficient region of the subject with the alpha-6 integrinhigh CD200high cell population, thereby stimulating a hair growth in a subject.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, the method comprising the steps of (a) isolating an alpha-6 integrinhigh CD200high cell population from a haired area of the subject; and (b) transplanting the alpha-6 integrinhigh CD200high cell population into a bald or hair-deficient region of the subject, thereby stimulating a hair growth in a subject.
As provided herein, an alpha-6 integrinhigh CD200high stem cell population is present in haired, but not bald scalp. Thus, this cell population is capable of generating new HF. In another embodiment, methods and compositions of the present invention utilize an integrinhigh CD200+ stem cell population. In another embodiment, an integrin+ CD200high stem cell population is used. In another embodiment, an integrin+ CD200+ stem cell population is used. Each possibility represents a separate embodiment of the present invention.
“Hair-deficient” refers, in another embodiment, to a region of the skin or scalp that is lacking an normal amount of hair. In another embodiment, the term refers to a region wherein the subject is dissatisfied with the amount of hair present. Each possibility represents a separate embodiment of the present invention.
“Alpha-6 integrinhigh CD200high cell population” refers, in another embodiment, to a cell population that exhibits an elevated alpha-6 integrin level, relative to other cells in the HF. In another embodiment, the population exhibits an elevated alpha-6 integrin level, relative to other cells in the epidermis. In another embodiment, the population exhibits is distinguishable by FACS from non-alpha-6 integrin− cells by FACS. In another embodiment, the population exhibits is distinguishable by FACS from non-alpha-6 integrinlow cells by FACS.
In another embodiment, the term refers to a cell population that exhibits an elevated CD200 level, relative to other cells in the HF. In another embodiment, the population exhibits an elevated CD200 level, relative to other cells in the epidermis. In another embodiment, the population exhibits is distinguishable by FACS from CD200low cells by FACS. In another embodiment, the population exhibits is distinguishable by FACS from CD200− cells by FACS.
Each definition of “alpha-6 integrinhigh CD200high cell” represents a separate embodiment of the present invention.
Methods are well known in the art for isolation of a cell population bearing 1 or more particular marker proteins. In another embodiment, fluorescence-activated cell sorting (FACS) is utilized (Examples herein). In another embodiment, antibody-coated magnetic beads are utilized. In another embodiment, any other method known in the art is utilized. Each possibility represents a separate embodiment of the present invention.
Methods of injecting, transporting, and administering cells into the skin or scalp of a subject are well known in the art, and are described, for example, in Zheng Y et al (Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells. J Invest Dermatol. 2005 May; 124(5):867-76) and in Example 9 herein. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of present invention further comprises the step of culturing ex vivo an alpha-6 integrinhigh CD200high cell population. In another embodiment, the cell population is expanded ex vivo. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing a level of a CD200 protein, thereby stimulating a hair growth in a subject. In another embodiment, increasing CD200 protein levels reduces inflammation in the scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a recombinant CD200 protein, thereby stimulating a hair growth in a subject. In another embodiment, a mimetic of the CD200 protein is administered. In another embodiment, an analogue of the CD200 protein is administered. Each possibility represents a separate embodiment of the present invention. In another embodiment, administration of a recombinant CD200 protein or a mimetic or analogue thereof reduces inflammation in the scalp. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of stimulating a hair growth in a subject, comprising the step of contacting the subject or the skin or scalp thereof with a compound or composition capable of increasing an activity of a CD200 protein, thereby stimulating a hair growth in a subject. In another embodiment, increasing CD200 protein activity reduces inflammation in the scalp. Each possibility represents a separate embodiment of the present invention.
As provided herein, an alpha-6 integrinhigh CD200high stem cell population is present in haired, but not bald scalp. Thus, CD200 and methods of upregulating same are efficacious in stimulation of hair growth.
The sequence of the alpha-6 integrin (ITGA6) of methods and compositions of the present invention is, in another embodiment:
MAAAGQLCLLYLSAGLLSRLGAAFNLDTREDNVIRKYGDPGSLFGFSLAMHWQLQ PEDKRLLLVGAPRAEALPLQRANRTGGLYSCDITARGPCTRIEFDNDADPTSESKEDQWMGVTVQ SQGPGGKVVTCAHRYEKRQHVNTKQESRDIFGRCYVLSQNLRIEDDMDGGDWSFCDGRLRGHE KFGSCQQGVAATFTKDFHYIVFGAPGTYNWKGIVRVEQKNNTFFDMNIFEDGPYEVGGETEHDES LVPVPANSYLGFSLDSGKGIVSKDEITFVSGAPRANHSGAVVLLKRDMKSAHLLPEHIFDGEGLAS SFGYDVAVVDLNKDGWQDIVIGAPQYFDRDGEVGGAVYVYMNQQGRWNNVKPIRLNGTKDSM FGIAVKNIGDINQDGYPDIAVGAPYDDLGKVFIYHGSANGINTKPTQVLKGISPYFGYSIAGNMDL DRNSYPDVAVGSLSDSVTIFRSRPVINIQKTITVTPNRIDLRQKTACGAPSGICLQVKSCFEYTANPA GYNPSISIVGTLEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLKRQKQKVCMEETLWLQDNIRD KLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSDEPKTAHIDVHFLKEGCGDDNVCNSNLKLEYKFC TREGNQDKFSYLPIQKGVPELVLKDQKDIALEITVTNSPSNPRNPTKDGDDAHEAKLIATFPDTLTY SAYRELRAFPEKQLSCVANQNGSQADCELGNPFKRNSNVTFYLVLSTTEVTFDTPDLDINLKLETT SNQDNLAPITAKAKVVIELLLSVSGVAKPSQVYFGGTVVGEQAMKSEDEVGSLIEYEFRVINLGKP LTNLGTATLNIQWPKEISNGKWLLYLVKVESKGLEKVTCEPQKEINSLNLTESHNSRKKREITEKQI DDNRKFSLFAERKYQTLNCSVNVNCVNIRCPLRGLDSKASLILRSRLWNSTFLEEYSKLNYLDILM RAFIDVTAAAENIRLPNAGTQVRVTVFPSKTVAQYSGVPWWIILVAILAGILMLALLVFILWKCGFF KRSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKKQWITKWNENESYS (SEQ ID No: 274; GenBank Accession No: NM—001079818). In another embodiment, the ITGA6 is a homologue of SEQ ID No: 276. In another embodiment, the ITGA6 is a variant of SEQ ID No: 276. In another embodiment, the ITGA6 is an isomer of SEQ ID No: 276. In another embodiment, the ITGA6 is a fragment of SEQ ID No: 276. In another embodiment, the ITGA6 comprises SEQ ID No: 276. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the ITGA6 has an AA sequence set forth in one of the following GenBank entries: NM—000210, BC050585, AH008066, AF166343, and L40385. In another embodiment, the ITGA6 has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the ITGA6 is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the ITGA6 is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the ITGA6 is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the ITGA6 is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the ITGA6 comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
The sequence of the CD200 of methods and compositions of the present invention is, in another embodiment:
MERLVIRMPFSHLSTYSLVWVMAAVVLCTAQVQVVTQDEREQLYTPASLKCSLQN AQEALIVTWQKKKAVSPENMVTFSENHGVVIQPAYKDKINITQLGLQNSTITFWNITLEDEGCYM CLFNTFGFGKISGTACLTVYVQPIVSLHYKFSEDHLNITCSATARPAPMVFWKVPRSGIENSTVTLS HPNGTTSVTSILHIKDPKNQVGKEVICQVLHLGTVTDFKQTVNKGYWFSVPLLLSIVSLVILLVLISI LLYWKRHRNQDREP (SEQ ID No: 275; GenBank Accession No: NM—005944). In another embodiment, the CD200 is a homologue of SEQ ID No: 275. In another embodiment, the CD200 is a variant of SEQ ID No: 275. In another embodiment, the CD200 is an isomer of SEQ ID No: 275. In another embodiment, the CD200 is a fragment of SEQ ID No: 275. In another embodiment, the CD200 comprises SEQ ID No: 275. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the sequence of the CD200 is:
MERLVIRMPFCHLSTYSLVWVMAAVVLCTAQVQVVTQDEREQLYTPASLKCSLQN AQEALIVTWQKKKAVSPENMVTFSENHGVVIQPAYKDKINITQLGLQNSTITFWNITLEDEGCYM CLFNTFGFGKISGTACLTVYVQPIVSLHYKFSEDHLNITCSATARPAPMVFWKVPRSGIENSTVTLS HPNGTTSVTSILHIKDPKNQVGKEVICQVLHLGTVTDFKQTVNKGYWFSVPLLLSIVSLVILLVLISI LLYWKRHRNQDREP (SEQ ID No: 276; GenBank Accession No: BC031103). In another embodiment, the CD200 is a homologue of SEQ ID No: 276. In another embodiment, the CD200 is a variant of SEQ ID No: 276. In another embodiment, the CD200 is an isomer of SEQ ID No: 276. In another embodiment, the CD200 is a fragment of SEQ ID No: 276. In another embodiment, the CD200 comprises SEQ ID No: 276. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the CD200 has an AA sequence set forth in one of the following GenBank entries NM—001004196, BC022522, AY603771, and AF495380. In another embodiment, the CD200 has a nucleotide sequence set forth in one of the above GenBank entries. In another embodiment, the CD200 is a homologue of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD200 is a variant of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD200 is an isomer of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD200 is a fragment of a sequence set forth in one of the above GenBank entries. In another embodiment, the CD200 comprises a sequence set forth in one of the above GenBank entries. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of the present invention utilizes a biologically active fragment of a protein product of a target gene of the present invention. In another embodiment, the protein product is CD200. CD200 biologically active fragments, analogues, and mimetics are well known in the art, and are described, for example, in patent applications US20040054145, WO 03/077947, WO 02/42332, US20040213783, and US20050084490. In another embodiment, the CD200 analogue is a soluble version of CD200. In another embodiment, the CD200 analogue is a CD200 fusion protein. In another embodiment, the CD200 analogue is a CD200Fc fusion protein. In another embodiment, a CD200R agonist is utilized in a method of the present invention. In another embodiment, the CD200R agonist is a monoclonal antibody. In another embodiment, the CD200 biologically active fragment, analogue, or mimetic or CD200R agonist is used in other applications (e.g. for immunosuppression for transplants). Each possibility represents a separate embodiment of the present invention.
In another embodiment, a mimetic compound of the present invention is derived from a target protein of the present invention by incorporating 1 or more modified AA residues. In another embodiment, one or more of the termini is derivatized to include a blocking group, i.e. a chemical substituent suitable to protect and/or stabilize the N- and C-termini from undesirable degradation. In another embodiment, “undesirable degradation” refers to any type of enzymatic, chemical or biochemical breakdown of the compound at its termini which is likely to affect the function of the compound, i.e. sequential degradation of the compound at a terminal end thereof.
In another embodiment, blocking groups include protecting groups conventionally used in the art of peptide chemistry which will not adversely affect the in vivo activities of the peptide. For example, suitable N-terminal blocking groups can be introduced by alkylation or acylation of the N-terminus. Examples of suitable N-terminal blocking groups include C1-C5 branched or unbranched alkyl groups, acyl groups such as formyl and acetyl groups, as well as substituted forms thereof, such as the acetamidomethyl (Acm) group. Desamino AA analogs are also useful N-terminal blocking groups, and can either be coupled to the N-terminus of the peptide or used in place of the N-terminal reside. Suitable C-terminal blocking groups, in which the carboxyl group of the C-terminus is either incorporated or not, include esters, ketones or amides. Ester or ketone-forming alkyl groups, particularly lower alkyl groups such as methyl, ethyl and propyl, and amide-forming amino groups such as primary amines (—NH2), and mono- and di-alkyl amino groups such as methyl amino, ethylamino, dimethylamino, diethylamino, methylethylamino and the like are examples of C-terminal blocking groups. Descarboxylated AA analogues such as agmatine are also useful C-terminal blocking groups and can be either coupled to the peptide's C-terminal residue or used in place of it. In another embodiment, the free amino and carboxyl groups at the termini are removed altogether from the peptide to yield desamino and descarboxylated forms thereof without affect on peptide activity.
In another embodiment, a mimetic compound of the present invention is derived from a target protein of the present invention by another modification. In another embodiment, such modifications include, but are not limited to, substitution of one or more of the AA in the natural L-isomeric form with D-isomeric AA. In another embodiment, the peptide includes one or more D-amino acid resides, or comprises AA that are all in the D-form. Retro-inverso forms of peptides in accordance with the present invention are also contemplated, for example, inverted peptides in which all amino acids are substituted with D-amino acid forms.
In another embodiment, mimetic compounds of the present invention are acid addition salts of a target protein of the present invention. In another embodiment, a peptide derived from a target protein of the present invention is treated with an inorganic acid such as hydrochloric, hydrobromic, sulfuric, nitric, phosphoric, and the like, or an organic acid such as an acetic, propionic, glycolic, pyruvic, oxalic, malic, malonic, succinic, maleic, fumaric, tataric, citric, benzoic, cinnamic, mandelic, methanesulfonic, ethanesulfonic, p-toluenesulfonic, salicyclic and the like, to provide a water soluble salt of the peptide suitable for use in the invention.
In another embodiment, a mimetic compound of the present invention is produced by a process comprising the step of in vivo or in vitro chemical derivatization of a peptide derived from a target protein of the present invention, e.g., acetylation, or carboxylation. Also included are modifications of glycosylation, e.g., those made by modifying the glycosylation patterns of a polypeptide during its synthesis and processing or in further processing steps; e.g., by exposing the polypeptide to enzymes which affect glycosylation, e.g., mammalian glycosylating or deglycosylating enzymes. In another embodiment, a mimetic compound of the present invention comprises a phosphorylated AA residue, e.g., phosphotyrosine, phosphoserine, or phosphothreonine.
In another embodiment, a mimetic compound of the present invention is produced by modifying a peptide derived from a target protein of the present invention using ordinary molecular biological techniques so as to improve it resistance to proteolytic degradation or to optimize solubility properties. In another embodiment, an peptide is modified to render it more suitable as a therapeutic agent. Analogs of such polypeptides include those containing residues other than naturally occurring L-amino acids, e.g., D-amino acids or non-naturally occurring synthetic amino acids. The peptides of the invention are not limited to products of any of the specific exemplary processes listed herein.
Methods of identifying mimetic compounds are well known in the art, and are described, for example, in Song J et al, Biochem Cell Biol 76(2-3): 177-188, 1998; Vogt A et al, J Biol Chem. 270(2): 660-4, 1995; Alexopoulos K et al, J Med Chem 47(13): 3338-52, 2004; Andronati S A et al, Curr Med Chem 11(9): 1183-211, 2004; Breslin M J et al, Bioorg Med Chem Lett 13(10): 1809-12, 2003; and WO 02/081649 (“ErbB interface peptidomimetics and methods of use thereof”) in the name of Greene et al. In another embodiment, model building is used to design the mimetic compounds as described in one of the above references. In another embodiment, solubility of the mimetic compounds is optimized as described in one of the above references. Each possibility represents a separate embodiment of the present invention.
Each modification, type of modification, and combination thereof represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a method of treating AGA, comprising performing a method of the present invention.
In another embodiment, the present invention provides a method of reversing AGA, comprising performing a method of the present invention.
In another embodiment, the present invention provides a method of generating new HF, comprising performing a method of the present invention.
In another embodiment, the present invention provides a method of stimulating formation of new HF, comprising performing a method of the present invention.
In another embodiment, the present invention provides a method of predicting the development of AGA in a subject, comprising the step of measuring an amount of PGD2 in the skin or scalp of the subject. In another embodiment, an amount of a PGD2 metabolite is measured. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of the present invention further comprises the step of contacting the subject with a pro-hair growth prostaglandin. In another embodiment, the scalp of the subject is contacted with the pro-hair growth prostaglandin. In another embodiment, the skin of the subject is contacted with the pro-hair growth prostaglandin. In another embodiment, the subject is contacted with the pro-hair growth prostaglandin, e.g. systemically, and the pro-hair growth prostaglandin reaches the target tissue via diffusion, active transport, or another biological process. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1 analogue. In another embodiment, the prostaglandin E1 analogue is Misoprostol (cytotec). In another embodiment, the prostaglandin E1 analogue is 11β-Misoprostol. In another embodiment, the prostaglandin E1 analogue is 8-iso Misoprostol. In another embodiment, the prostaglandin E1 analogue is Mifepristone. In another embodiment, the prostaglandin E1 analogue is Misoprostol (free acid). In another embodiment, the prostaglandin E1 analogue is Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 11β-Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 13,14-dihydro-15-keto Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 13,14-dihydro-15-keto Prostaglandin E1-d4. In another embodiment, the prostaglandin E1 analogue is 13,14-dihydro-19(R)-hydroxy Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 15-keto Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 16,16-dimethyl-6-keto Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 16-phenyl tetranor Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 19(R)-hydroxy Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 2,3-dinor Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is 8-iso Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is Bicyclo Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is Dihomo-γ-Linolenic Acid. In another embodiment, the prostaglandin E1 analogue is Limaprost. In another embodiment, the prostaglandin E1 analogue is Δ17-Prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is selected from 11β-Misoprostol, 8-iso Misoprostol, Mifepristone, Misoprostol (free acid), Prostaglandin E1, 11β-Prostaglandin E1, 13,14-dihydro-15-keto Prostaglandin E1, 13,14-dihydro-15-keto Prostaglandin E1-d4, 13,14-dihydro-19(R)-hydroxy Prostaglandin E1, 15-keto Prostaglandin E1, 16,16-dimethyl-6-keto Prostaglandin E1, 16-phenyl tetranor Prostaglandin E1, 19(R)-hydroxy Prostaglandin E1, 2,3-dinor Prostaglandin E1, 8-iso Prostaglandin E1, Bicyclo Prostaglandin E1, Dihomo-γ-Linolenic Acid, Limaprost, and Δ17-Prostaglandin E1. In another embodiment, the prostaglandin E1 analogue is selected from 11β-Misoprostol, 8-iso Misoprostol, Mifepristone, Misoprostol (free acid), Prostaglandin E1, 11β-Prostaglandin E1, 13,14-dihydro-15-keto Prostaglandin E1, 13,14-dihydro-15-keto Prostaglandin E1-d4, 13,14-dihydro-19(R)-hydroxy Prostaglandin E1, 15-keto Prostaglandin E1, 16,16-dimethyl-6-keto Prostaglandin E1, 16-phenyl tetranor Prostaglandin E1, 19(R)-hydroxy Prostaglandin E1, 2,3-dinor Prostaglandin E1, 8-iso Prostaglandin E1, Bicyclo Prostaglandin E1, Dihomo-γ-Linolenic Acid, Limaprost, and Δ17-Prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin E1 analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other agonistic prostaglandin E1 analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin E1 analogue known in the art that mimics the action of prostaglandin E1. In another embodiment, a compound or composition that increases prostaglandin E1 levels or prostaglandin E1 synthesis is administered to the subject. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2 analogue. In another embodiment, the pro-hair growth prostaglandin is 11-deoxy-16,16-dimethyl Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 11-deoxy Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 11β-Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 13,14-dihydro-15-keto Prostaglandin E1-d4. In another embodiment, the pro-hair growth prostaglandin is 13,14-dihydro Prostaglandin E1-d4. In another embodiment, the pro-hair growth prostaglandin is 15(R)-15-methyl Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 15(R)-Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 15(S)-15-methyl Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 16-phenoxy tetranor Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 16-phenyl tetranor Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 20-ethyl Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 5-trans Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 8-iso Prostaglandin E2 isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is 9-deoxy-9-methylene-16,16-dimethyl Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is 9-deoxy-9-methylene Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is ent-Prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin E2-biotinimide. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin E2-d4. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin E2 methyl ester. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin E2 serinol amide. In another embodiment, the pro-hair growth prostaglandin is Sulprostone. In another embodiment, the pro-hair growth prostaglandin is selected from 11-deoxy-16,16-dimethyl Prostaglandin E2, 11-deoxy Prostaglandin E2, 11β-Prostaglandin E2, 13,14-dihydro-15-keto Prostaglandin E1-d4, 13,14-dihydro Prostaglandin E1-d4, 15(R)-15-methyl Prostaglandin E2, 15(R)-Prostaglandin E2, 15(S)-15-methyl Prostaglandin E2, 16-phenoxy tetranor Prostaglandin E2, 16-phenyl tetranor Prostaglandin E2, 20-ethyl Prostaglandin E2, 5-trans Prostaglandin E2, 8-iso Prostaglandin E2 isopropyl ester, 9-deoxy-9-methylene-16,16-dimethyl Prostaglandin E2, 9-deoxy-9-methylene Prostaglandin E2, ent-Prostaglandin E2, Prostaglandin E2-biotinimide, Prostaglandin E2-d4, Prostaglandin E2 methyl ester, Prostaglandin E2 serinol amide, and Sulprostone. In another embodiment, the prostaglandin E2 analogue is selected from 11-deoxy-16,16-dimethyl Prostaglandin E2, 11-deoxy Prostaglandin E2, 11β-Prostaglandin E2, 13,14-dihydro-15-keto Prostaglandin E1-d4, 13,14-dihydro Prostaglandin E1-d4, 15(R)-15-methyl Prostaglandin E2, 15(R)-Prostaglandin E2, 15(S)-15-methyl Prostaglandin E2, 16-phenoxy tetranor Prostaglandin E2, 16-phenyl tetranor Prostaglandin E2, 20-ethyl Prostaglandin E2, 5-trans Prostaglandin E2, 8-iso Prostaglandin E2 isopropyl ester, 9-deoxy-9-methylene-16,16-dimethyl Prostaglandin E2, 9-deoxy-9-methylene Prostaglandin E2, ent-Prostaglandin E2, Prostaglandin E2-biotinimide, Prostaglandin E2-d4, Prostaglandin E2 methyl ester, Prostaglandin E2 serinol amide, and Sulprostone. In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin E2 analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other agonistic prostaglandin E2 analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin E2 analogue known in the art that mimics the action of prostaglandin E2. In another embodiment, a compound or composition that increases prostaglandin E2 levels or prostaglandin E2 synthesis is administered to the subject. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a analogue. In another embodiment, the pro-hair growth prostaglandin is Latanoprost (Xalatan). In another embodiment, the pro-hair growth prostaglandin is Isopropyl unoprostone (Rescula). In another embodiment, the pro-hair growth prostaglandin is 11-deoxy Prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is 11β-13,14-dihydro-15-keto Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 11β-Prostaglandin F2α-d4. In another embodiment, the pro-hair growth prostaglandin is 11β-Prostaglandin F2α Ethanolamide. In another embodiment, the pro-hair growth prostaglandin is 13,14-dihydro-15-keto Prostaglandin F2α isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is 15-keto Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 15(R)-Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 16,16-dimethyl Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 2,3-dinor-11β-Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 2,3-dinor-8-iso Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is 8-iso-15(R)-Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is AL 8810 isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is CAY10509 (Cayman Chemical, Ann Arbor, Mich.). In another embodiment, the pro-hair growth prostaglandin is CAY10510 (Cayman Chemical). In another embodiment, the pro-hair growth prostaglandin is Cloprostenol (sodium salt). In another embodiment, the pro-hair growth prostaglandin is ent-Prostaglandin F2α. In another embodiment, the pro-hair growth prostaglandin is Latanoprost-d4. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α Alcohol. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α-d4. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α-d9. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α diethyl amide. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α dimethyl amide. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α dimethyl amine. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α ethyl amide. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α methyl ester. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α serinol amide. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α (tromethamine salt). In another embodiment, the pro-hair growth prostaglandin is selected from Latanoprost (Xalatan), Isopropyl unoprostone (Rescula), 11-deoxy Prostaglandin F2α, 11β-13,14-dihydro-15-keto Prostaglandin F2α, 11β-Prostaglandin F2α-d4, 11β-Prostaglandin F2α Ethanolamide, 13,14-dihydro-15-keto Prostaglandin F2α isopropyl ester, 15-keto Prostaglandin F2α, 15(R)-Prostaglandin F2α, 16,16-dimethyl Prostaglandin F2α, 2,3-dinor-11β-Prostaglandin F2α, 2,3-dinor-8-iso Prostaglandin F2α, 8-iso-15(R)-Prostaglandin F2α, AL8810 isopropyl ester, CAY10509, CAY10510, Cloprostenol (sodium salt), ent-Prostaglandin F2α, Latanoprost-d4, Prostaglandin F2α Alcohol, Prostaglandin F2α-d4, Prostaglandin F2α-d9, Prostaglandin F2α diethyl amide Prostaglandin F2α dimethyl amide, Prostaglandin F2α dimethyl amine, Prostaglandin F2α ethyl amide, Prostaglandin F2α isopropyl ester, Prostaglandin F2α methyl ester, Prostaglandin F2α serinol amide, Prostaglandin F2α (tromethamine salt). In another embodiment, the prostaglandin F2α analogue is selected from Latanoprost (Xalatan), Isopropyl unoprostone (Rescula), 11-deoxy Prostaglandin F2α, 11β-13,14-dihydro-15-keto Prostaglandin F2α, 11β-Prostaglandin F2α-d4, 11β-Prostaglandin F2α Ethanolamide, 13,14-dihydro-15-keto Prostaglandin F2α isopropyl ester, 15-keto Prostaglandin F2α, 15(R)-Prostaglandin F2α, 16,16-dimethyl Prostaglandin F2α, 2,3-dinor-11β-Prostaglandin F2α, 2,3-dinor-8-iso Prostaglandin F2α, 8-iso-15(R)-Prostaglandin F2α, AL 8810 isopropyl ester, CAY10509, CAY10510, Cloprostenol (sodium salt), ent-Prostaglandin F2α, Latanoprost-d4, Prostaglandin F2α Alcohol, Prostaglandin F2α-d4, Prostaglandin F2α-d9, Prostaglandin F2α diethyl amide, Prostaglandin F2α dimethyl amide, Prostaglandin F2α dimethyl amine, Prostaglandin F2α ethyl amide, Prostaglandin F2α isopropyl ester, Prostaglandin F2α methyl ester, Prostaglandin F2α serinol amide, Prostaglandin F2α (tromethamine salt). In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin F2a analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other agonistic prostaglandin F2a analogue known in the art. In another embodiment, the pro-hair growth prostaglandin is any other prostaglandin F2a analogue known in the art that mimics the action of prostaglandin F2a. In another embodiment, a compound or composition that increases prostaglandin F2a levels or prostaglandin F2a synthesis is administered to the subject. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the pro-hair growth prostaglandin is 11-keto Fluprostenol. In another embodiment, the pro-hair growth prostaglandin is 15-keto Latanoprost. In another embodiment, the pro-hair growth prostaglandin is 15-keto Latanoprost (free acid). In another embodiment, the pro-hair growth prostaglandin is 15(S)-Latanoprost. In another embodiment, the pro-hair growth prostaglandin is 5-trans Latanoprost. In another embodiment, the pro-hair growth prostaglandin is 9-keto Fluprostenol. In another embodiment, the pro-hair growth prostaglandin is Fluprostenol. In another embodiment, the pro-hair growth prostaglandin is Latanoprost-d4. In another embodiment, the pro-hair growth prostaglandin is Latanoprost ethyl amide-d4. In another embodiment, the pro-hair growth prostaglandin is Latanoprost (free acid). In another embodiment, the pro-hair growth prostaglandin is Latanoprost Lactol. In another embodiment, the pro-hair growth prostaglandin is Lumula. In another embodiment, the pro-hair growth prostaglandin is Prostaglandin F2α isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is Unoprostone. In another embodiment, the pro-hair growth prostaglandin is Unoprostone isopropyl ester. In another embodiment, the pro-hair growth prostaglandin is selected from 11-keto Fluprostenol, 15-keto Latanoprost, 15-keto Latanoprost (free acid), 15(S)-Latanoprost, 5-trans Latanoprost, 9-keto Fluprostenol, Fluprostenol, Latanoprost-d4, Latanoprost ethyl amide-d4, Latanoprost (free acid), Latanoprost Lactol, Lumula, Prostaglandin F2α isopropyl ester, Unoprostone, Unoprostone isopropyl ester.
In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and an inhibitor of prostaglandin D2 activity. In another embodiment, the pharmaceutical composition further comprises a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and an downregulator of prostaglandin D2 levels. In another embodiment, the pharmaceutical composition further comprises a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a COX-2 inhibitor and a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising an inhibitor of prostaglandin D2 activity and a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a pharmaceutical composition, comprising a downregulator of prostaglandin D2 levels and a pro-hair growth prostaglandin. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E1. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin E2. In another embodiment, the pro-hair growth prostaglandin is a prostaglandin F2a. In another embodiment, the pro-hair growth prostaglandin is any other pro-hair growth prostaglandin known in the art. Each possibility represents a separate embodiment of the present invention.
In another embodiment, a method of present invention further comprises the step of disrupting the epidermis of the target scalp, eyebrow, or scarred region. In another embodiment, a method of present invention further comprises the step of abrading the target scalp, eyebrow, or scarred region. In another embodiment, a method of present invention further comprises the step of wounding the target scalp, eyebrow, or scarred region. In another embodiment, the epidermal disruption, wounding, or abrading is performed prior to a step of a method of the present invention. In another embodiment, the epidermal disruption, wounding, or abrading is performed simultaneously with a step of a method of the present invention. In another embodiment, the epidermal disruption, wounding, or abrading is performed concurrently with a step of a method of the present invention. In another embodiment, the epidermal disruption, wounding, or abrading is performed after a step of a method of the present invention. Methods for disrupting the epidermis, wounding, and abrading are well known in the art, and are described, for example in PCT International Application Serial No. PCT/US2006/011319, which is incorporated herein by reference. Each possibility represents a separate embodiment of the present invention.
In another embodiment of methods and compositions of the present invention, a scalp or skin in need is contacted with a compound or composition capable of inhibiting a prostaglandin D2 activity. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of inhibiting a signaling or receptor pathway downstream of prostaglandin D2. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of decreasing a level of a prostaglandin D2. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of inhibiting an activity of a prostaglandin D2 synthase enzyme. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of decreasing a level of a prostaglandin D2 synthase enzyme. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of increasing an activity of a target gene of the present invention. In another embodiment, a scalp or skin in need is contacted with a compound or composition capable of increasing an expression of a target gene of the present invention. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the scalp or skin is directly contacted. In another embodiment, a compound or composition of the present invention is applied directly to the scalp or skin. In another embodiment, the scalp or skin is contacted indirectly. In another embodiment, a compound or composition of the present invention is administered to the subject, and then is brought into contact with the scalp or skin in need by a transport process. In another embodiment, the transport process is passive transport. In another embodiment, the transport process is active transport. In another embodiment, the transport process is a combination of passive and active transport. In another embodiment, the transport process is diffusion. In another embodiment, the transport process is mediated by the circulatory system. In another embodiment, the transport process is mediated by the lymph system. In another embodiment, the transport process is mediated by any other system known in the art. Each possibility represents a separate embodiment of the present invention.
In one embodiment, a scalp is treated by a method of the present invention. In another embodiment, an eyebrow is treated. In another embodiment, a scarred region is treated. In another embodiment, any other hair-bearing area or region of the skin is treated. Each possibility represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to inhibit a prostaglandin D2 activity in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to inhibit a signaling or receptor pathway downstream of prostaglandin D2 in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to decrease the prostaglandin D2 level in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to inhibit prostaglandin D2 accumulation in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to inhibit an activity of a prostaglandin D2 synthase in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to decrease a level of a prostaglandin D2 synthase enzyme in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to increase an activity or level of a target gene of the present invention in the subject's scalp.
In another embodiment, the present invention provides a composition for treating baldness, the composition comprising a compound or composition that is effective to decrease an activity or level of a target gene of the present invention in the subject's scalp.
In another embodiment, a protein or gene of the present invention is homologous to a peptide disclosed herein. The terms “homology,” “homologous,” etc, when in reference to any protein or peptide, refer, in one embodiment, to a percentage of amino acid residues in the candidate sequence that are identical with the residues of a corresponding native polypeptide, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent homology, and not considering any conservative substitutions as part of the sequence identity. Methods and computer programs for the alignment are well known in the art.
Homology is, in another embodiment, determined by computer algorithm for sequence alignment, by methods well described in the art. For example, computer algorithm analysis of nucleic acid sequence homology can include the utilization of any number of software packages available, such as, for example, the BLAST, DOMAIN, BEAUTY (BLAST Enhanced Alignment Utility), GENPEPT and TREMBL packages.
In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 70%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 72%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 75%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 78%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 80%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 82%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 83%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 85%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 87%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 88%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 90%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 92%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 93%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 95%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 1-251 of greater than 96%. In another embodiment, “homology” refers, to identity to one of SEQ ID No: 1-251 of greater than 97%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 98%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of greater than 99%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 1-251 of 100%. Each possibility represents a separate embodiment of the present invention.
In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 70%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 72%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 75%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 78%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 80%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 82%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 83%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 85%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 87%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 88%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 90%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 92%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 93%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 95%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 252-276 of greater than 96%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 97%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 98%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of greater than 99%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 252-276 of 100%. Each possibility represents a separate embodiment of the present invention.
In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 70%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 72%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 75%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 78%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 80%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 82%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 83%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 85%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 87%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 88%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 90%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 92%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 93%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 95%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 277-303 of greater than 96%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 97%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 98%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of greater than 99%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 277-303 of 100%. Each possibility represents a separate embodiment of the present invention.
In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 70%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 72%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 75%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 78%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 80%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 82%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 83%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 85%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 87%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 88%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 90%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 92%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No; 304-317 of greater than 93%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 95%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 304-317 of greater than 96%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 97%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 98%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of greater than 99%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 304-317 of 100%. Each possibility represents a separate embodiment of the present invention.
In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 70%. In another embodiment; “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 72%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 75%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 78%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 80%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 82%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 83%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 85%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 87%. In another embodiment, “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 88%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 90%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 92%. In another embodiment, “homology” referS to identity to a sequence selected from SEQ ID No: 318-323 of greater than 93%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 95%. In another embOdiment, “homology” refers to identity to a sequence selected from SEQ ID No: 318-323 of greater than 96%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 97%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 98%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of greater than 99%. In another embodiment, “homology” refers to identity to one of SEQ ID No: 318-323 of 100%. Each possibility represents a separate embodiment of the present invention.
In another embodiment, homology is determined via determination of candidate sequence hybridization, methods of which are well described in the art (See, for example, “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., Eds. (1985); Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y). In other embodiments, methods of hybridization are carried out under moderate to stringent conditions, to the complement of a DNA encoding a native caspase peptide. Hybridization conditions being, for example, overnight incubation at 42° C. in a solution comprising: 10-20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA.
In another embodiment of the present invention, “nucleic acids” or “nucleotide” refers to a string of at least two base-sugar-phosphate combinations. The term includes, in one embodiment, DNA and RNA. “Nucleotides” refers, in one embodiment, to the monomeric units of nucleic acid polymers. RNA is, in one embodiment, in the form of a tRNA (transfer RNA), snRNA (small nuclear RNA), rRNA (ribosomal RNA), mRNA (messenger RNA), anti-sense RNA, small inhibitory RNA (siRNA), micro RNA (miRNA) and ribozymes. The use of siRNA and miRNA has been described (Caudy A A et al, Genes & Devel 16: 2491-96 and references cited therein). DNA can be, in other embodiments, in form of plasmid DNA, viral DNA, linear DNA, or chromosomal DNA or derivatives of these groups. In addition, these forms of DNA and RNA can be single, double, triple, or quadruple stranded. The term also includes, in another embodiment, artificial nucleic acids that contain other types of backbones but the same bases. In one embodiment, the artificial nucleic acid is a PNA (peptide nucleic acid). PNA contain peptide backbones and nucleotide bases and are able to bind, in one embodiment, to both DNA and RNA molecules. In another embodiment, the nucleotide is oxetane modified. In another embodiment, the nucleotide is modified by replacement of one or more phosphodiester bonds with a phosphorothioate bond. In another embodiment, the artificial nucleic acid contains any other variant of the phosphate backbone of native nucleic acids known in the art. The use of phosphothiorate nucleic acids and PNA are known to those skilled in the art, and are described in, for example, Neilsen P E, Curr Opin Struct Biol 9:353-57; and Raz N K et al Biochem Biophys Res Commun. 297:1075-84. The production and use of nucleic acids is known to those skilled in art and is described, for example, in Molecular Cloning, (2001), Sambrook and Russell, eds. and Methods in Enzymology: Methods for molecular cloning in eukaryotic cells (2003) Purchio and G. C. Fareed. Each nucleic acid derivative represents a separate embodiment of the present invention.
Protein and/or peptide homology for any amino acid sequence listed herein is determined, in another embodiment, by methods well described in the art, including immunoblot analysis, or via computer algorithm analysis of amino acid sequences, utilizing any of a number of software packages available, via established methods. Some of these packages include the FASTA, BLAST, MPsrch or Scanps packages, and, in another embodiment, employ the use of the Smith and Waterman algorithms, and/or global/local or BLOCKS alignments for analysis, for example. Each method of determining homology represents a separate embodiment of the present invention.
In another embodiment, the present invention provides a kit comprising a reagent utilized in performing a method of the present invention. In another embodiment, the present invention provides a kit comprising a composition, tool, or instrument of the present invention.
Pharmaceutical Compositions and Methods of Administration
In another embodiment, methods of the present invention comprise the step of administering a pharmaceutical composition containing a compound or composition of the present invention. “Pharmaceutical composition” refers, in another embodiment, to a therapeutically effective amount of the active ingredient, together with a pharmaceutically acceptable carrier or diluent. A “therapeutically effective amount” refers, in one embodiment, to that amount which provides a therapeutic effect for a given condition and administration regimen.
The pharmaceutical compositions containing the compound or composition can be, in another embodiment, administered to a subject by any method known to a person skilled in the art, such as parenterally, paracancerally, transmucosally, transdermally, intramuscularly, intravenously, intra-dermally, subcutaneously, intra-peritonealy, intra-ventricularly, intra-cranially, intra-vaginally or intra-tumorally.
In another embodiment, the pharmaceutical composition is administered topically to body surfaces and is thus formulated in a form suitable for topical administration. In another embodiment, the pharmaceutical composition is administered to the target scalp, skin, or hair-bearing region. Suitable topical formulations include, in another embodiment, gels, ointments, creams, lotions, drops and the like. For topical administration, the compound or composition is prepared and applied as a solution, suspension, or emulsion in a physiologically acceptable diluent with or without a pharmaceutical carrier.
In another embodiment of methods and compositions of the present invention, the pharmaceutical compositions are administered orally, and are thus formulated in a form suitable for oral administration, i.e. as a solid or a liquid preparation. Suitable solid oral formulations include tablets, capsules, pills, granules, pellets and the like. Suitable liquid oral formulations include solutions, suspensions, dispersions, emulsions, oils and the like. In another embodiment of the present invention, the active ingredient is formulated in a capsule. In accordance with this embodiment, the compositions of the present invention comprise, in addition to the active compound and the inert carrier or diluent, a hard gelating capsule.
In another embodiment, the pharmaceutical compositions are administered by intravenous, intra-arterial, or intra-muscular injection of a liquid preparation. Suitable liquid formulations include solutions, suspensions, dispersions, emulsions, oils and the like. In another embodiment, the pharmaceutical compositions are administered intravenously and are thus formulated in a form suitable for intravenous administration. In another embodiment, the pharmaceutical compositions are administered intra-arterially and are thus formulated in a form suitable for intra-arterial administration. In another embodiment, the pharmaceutical compositions are administered intra-muscularly and are thus formulated in a form suitable for intra-muscular administration.
In another embodiment, the pharmaceutical composition is administered as a suppository, for example a rectal suppository or a urethral suppository. In another embodiment, the pharmaceutical composition is administered by subcutaneous implantation of a pellet. In another embodiment, the pellet provides for controlled release of active agent over a period of time.
In another embodiment, the active compound is delivered in a vesicle, e.g. a liposome.
In other embodiments, carriers or diluents used in methods of the present invention include, but are not limited to, a gum, a starch (e.g. corn starch, pregeletanized starch), a sugar (e.g., lactose, mannitol, sucrose, dextrose), a cellulosic material (e.g. microcrystalline cellulose), an acrylate (e.g. polymethylacrylate), calcium carbonate, magnesium oxide, talc, or mixtures thereof.
In other embodiments, pharmaceutically acceptable carriers for liquid formulations are aqueous or non-aqueous solutions, suspensions, emulsions or oils. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Examples of oils are those of animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, olive oil, sunflower oil, fish-liver oil, another marine oil, or a lipid from milk or eggs.
In another embodiment, parenteral vehicles (for subcutaneous, intravenous, intraarterial, or intramuscular injection) include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Examples are sterile liquids such as water and oils, with or without the addition of a surfactant and other pharmaceutically acceptable adjuvants. In general, water, saline, aqueous dextrose and related sugar solutions, and glycols such as propylene glycols or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions. Examples of oils are those of animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, olive oil, sunflower oil, fish-liver oil, another marine oil, or a lipid from milk or eggs.
In other embodiments, the compositions further comprise binders (e.g. acacia, cornstarch, gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, povidone), disintegrating agents (e.g. cornstarch, potato starch, alginic acid, silicon dioxide, croscarmelose sodium, crospovidone, guar gum, sodium starch glycolate), buffers (e.g., Tris-HCL, acetate, phosphate) of various pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween 20, Tween 80, Pluronic F68, bile acid salts), protease inhibitors, surfactants (e.g. sodium lauryl sulfate), permeation enhancers, solubilizing agents (e.g., glycerol, polyethylene glycerol), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite, butylated hydroxyanisole), stabilizers (e.g. hydroxypropyl cellulose, hyroxypropylmethyl cellulose), viscosity increasing agents (e.g. carbomer, colloidal silicon dioxide, ethyl cellulose, guar gum), sweeteners (e.g. aspartame, citric acid), preservatives (e.g., Thimerosal, benzyl alcohol, parabens), lubricants (e.g. stearic acid, magnesium stearate, polyethylene glycol, sodium lauryl sulfate), flow-aids (e.g. colloidal silicon dioxide), plasticizers (e.g. diethyl phthalate, triethyl citrate), emulsifiers (e.g. carbomer, hydroxypropyl cellulose, sodium lauryl sulfate), polymer coatings (e.g., poloxamers or poloxamines), coating and film forming agents (e.g. ethyl cellulose, acrylates, polymethacrylates) and/or adjuvants. Each of the above excipients represents a separate embodiment of the present invention.
In another embodiment, the pharmaceutical compositions provided herein are controlled-release compositions, i.e. compositions in which the active compound is released over a period of time after administration. Controlled- or sustained-release compositions include formulation in lipophilic depots (e.g. fatty acids, waxes, oils). In another embodiment, the composition is an immediate-release composition, i.e. a composition in which all the active compound is released immediately after administration.
The preparation of pharmaceutical compositions that contain an active component, for example by mixing, granulating, or tablet-forming processes, is well understood in the art. The active therapeutic ingredient is often mixed with excipients that are pharmaceutically acceptable and compatible with the active ingredient. For oral administration, the active agent is mixed with additives customary for this purpose, such as vehicles, stabilizers, or inert diluents, and converted by customary methods into suitable forms for administration, such as tablets, coated tablets, hard or soft gelatin capsules, aqueous, alcoholic or oily solutions. For parenteral administration, the active agent is converted into a solution, suspension, or emulsion, if desired with the substances customary and suitable for this purpose, for example, solubilizers or other substances.
Each of the above additives, excipients, formulations and methods of administration represents a separate embodiment of the present invention.
Experimental Details Section
Example 1
Histopathology of Androgenetic Alopecia
Materials and Experimental Methods (Examples 1-5)
Tissue Samples
Human scalp samples were obtained from 8 subjects during hair transplantation, with approval of the University of Pennsylvania Institutional Review Board. During the procedure, 2 millimeter mm wide by several centimeter (cm) long specimens are taken from the donor scalp, and then dissected into various graft sizes (single hair grafts to multiple hair grafts). Some tissue is deemed unsatisfactory for transplantation, due to inadvertent sectioning, for example. These discarded donor samples were used as haired specimens. Likewise, 1.2 mm and 1.7 mm diameter bald scalp cylindrical “punches” are performed to create vacant recipient sites for donor scalp, which was used as the bald samples. Subjects were 40-63 year old Caucasian males not using using minoxidil or finasteride. For RNA isolation, paired samples from 5 patients were stored in RNA Later® (Qiagen). For flow cytometry, paired samples from 3 patients were kept at 4° C. in DMEM+streptomycin and fungazone (GIBCO) until use. Scalp excisions used for in situ hybridization were provided by the University of Pennsylvania Human Cooperative Tissue Network.
Flow Cytometry
Samples were treated with 2.5 U/ml of Dispase (Sigma) in DMEM with 0.3% FBS, gentamycin and 0.01M HEPES overnight. The epidermis was gently removed from the dermis, with simultaneous removal of hair shafts. With removal of individual hair shafts, the surrounding follicle keratinocytes were likewise separated from dermis. Bald scalp follicles were also carefully separated individually. Isolated epidermis was then washed in PBS, and treated with Trypsin for 20 minutes at 37° C. in the presence of DNAase. The sample was vortexed gently and filtered trough a sterile 70 μm filter. Cells were spun at 200×g for 5 minutes and washed twice with PBS. Cells were counted using a hemocytometer. For every stage of the above procedure, tissue was saved for monitoring of successful purification by hematoxylin and eosin staining. 600,000 cells were used for counting each sample with attempts made to quantify the maximum possible during flow analysis. Samples were stained with antibodies to alpha-6 integrin (Pharmingen, San Jose, Calif., Clone GoH3), fixed and permeabilized (Caltag labs, Burlingame, Calif.), and stained with antibodies against ACTIN (Sigma clone AC-15), KRT15 (Lab Vision clone LHK15), FST (R&D Systems Clone 85918) and with an isotype control (IgG2a Sigma). Isotype, KRT15 and FST antibodies were pre-labeled with chromophores preconjugatcd to Fab (Zenon Tricolor IgG2a kit). The antibody to ACTIN was directly conjugated to FITC by the manufacturer. Cells were counted on a Becton Dickson FACScalibur® machine using the Cell Quest® application (BD Biosciences, San Jose Calif.). FITC, PE and PerCP channels were detected using the respective bandpass filters (530/30, 575/26, 695/45), after excitation from a 488 15 mW argon laser. Data was analyzed with FloJo® (Treestar) software. For each patient tested, identical gating was used for all samples using the same antibodies.
Target Preparation and Hybridization
5 microgram (mcg) total RNA was converted to first-strand cDNA using Superscript II reverse transcriptase primed by a poly(T) oligomer that incorporated the T7 promoter. Second-strand cDNA synthesis was followed by in vitro transcription for linear amplification of each transcript and incorporation of biotinylated CTP and UTP. The cRNA products were fragmented to 200 nucleotides or less, heated at 99° C. for 5 min and hybridized for 16 h at 45° C. to U133A Affymetrix microarrays (Human Genome U133A Array). The microarrays were then washed at low (6×SSPE) and high (100 millimolar (mM) MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain. A confocal scanner was used to collect fluorescence signal at 3 micrometer (mcm) resolution after excitation at 570 nm. The average signal from 2 sequential scans was calculated for each microarray feature.
Initial Data Analysis
Affymetrix GeneChip® Operating System (GCOS, v. 1.4, Affymetrix, Inc.) was used to quantitate expression signal levels for the arrays; default values provided by Affymetrix were applied to all analysis parameters. Border pixels were removed, and the average intensity of pixels within the 75th percentile was computed for each probe. These values were exported as .cel files. The average of the lowest 2% of probe intensities occurring in each of 16 microarray sectors was set as background and subtracted from all features in that sector. Probe pairs were scored positive or negative for detection of the targeted sequence by comparing signals from the perfect match and mismatch probe features. The number of probe pairs meeting the default discrimination threshold (tau=0.015) was used to assign a call of absent, present or marginal for each assayed gene, and a p-value was calculated to reflect confidence in the detection call. The flag values were additionally exported in .chp files.
Affymetrix probe intensities were imported into GeneSpring® (v7.2, Agilent Technologies) where probeset signal values were calculated using the GC-RMA algorithm. Upon import of the Affymetrix flag values, the probesets were filtered to retain only those that were flagged P (present) in at least two of the 10 samples. This list was used for condition-based principle components analysis (PCA) to assess global trends in sample similarity. This analysis demonstrated groupings based on both patient and processing date, and prompted the use of mixed-model 3-way ANOVA as a means of finding differentially regulated genes between the sites of interest.
GC-RMA signal data was imported into Partek Genomics Solution® (PGS, v6.2, Partek, Inc.), where the data were log2 transformed, and a 3-way mixed model ANOVA was applied. Location (bald or haired) was considered a fixed effect, while patient and processing date were considered random effects. Probesets were ranked by ascending p-values for the “location” term of the ANOVA, thereby prioritizing genes that best differentiated between bald and haired scalp. The top 250 probesets, having a false discovery rate (FDR, by the “step-down” method implemented in PGS) of less than 18% were retained for further analysis. This list was imported to GeneSpring® for further analysis, visualization and hierarchical clustering of both genes and samples. Fold change was calculated by taking the geometric mean of the 5 haired/bald patient-paired ratios for each gene (using GC-RMA signal values). Positive fold changes indicate greater relative expression in the haired scalp, and negative values indicate greater expression in the bald scalp. 169 probesets were found to be higher in haired scalp, and 81 were found to be higher in bald scalp.
As a further validation of the relationship among the samples, a 10×10 pairwise correlation matrix was calculated using log2 transformed GC-RMA data from which the batch effects of patient and processing date had been removed (PGS, using the same 3-way mixed model ANOVA described above). The mean and standard deviation for all haired-haired, haired-bald and bald-bald paired correlations were calculated (excluding self-self pairs).
Annotations for the 251 probesets were mined using the DAVID functional annotation tool (Dennis G et al. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003; 4(5):P3 The lists of 169 and 81 genes were analyzed separately, and were scored for their overrepresentation of Gene Ontology Biological Processes (GO_BP_All) categories with a minimum of 5 genes per category.
In Situ Hybridization
All primers used to generate probes are depicted in FIG. 8. Anti-sense PCR-derived probe templates were constructed to contain the T7 promoter. Amplified probe templates were purified in agarose gel via QIAquick® extraction kit (Qiagen, Chatsworth, Calif.). Purified products were converted to digoxin-containing ribroprobes with the DIG RNA labeling kit (Roche, Indianapolis, Ind.) according to the manufacturer's instructions. Paraformaldehyde (PFA)-fixed, paraffin-embedded tissue from scalp excisions from haired scalp. No tissue from patients participating in array or flow cytometry experiments was used. Embedded tissue was removed of paraffin with xylene and rehydrated. Tissue was permeabilized with proteinase K (Roche, Indianapolis, Ind.) and re-fixed in 4% PFA followed by acetylation in acetic anhydride and triethanolamine. Sections were pre-hybridized for 2 hours, and then hybridized with 500-1000 ng/ml of probe for 16 hours. Slides were subsequently washed, blocked, treated with antibodies to digoxin preconjugated to alkaline phosphatase (Roche, Indianapolis, Ind.), and developed with NBT and BCIP (Roche, Indianapolis, Ind.).
Immunohistochemistry
Tissue was from different subjects than those used for microarray or flow cytometry experiments. Briefly, PFA-fixed, paraffin-embedded slides containing human scalp hair from excisions were de-parafinized and rehydrated. Microwave antigen retrieval was used with 10 mM citrate buffer (pH 6.8). Slides were treated with hydrogen peroxidase to block endogenous horseradish peroxidase activity, washed, and treated with blocking reagent and then primary antibody (chicken anti-KRT15 at 1:200, Covance), washed, treated with secondary biotinylated anti-chicken antibody (Kirkegaard and Perry Laboratories, Inc), washed, treated with streptavidin-HRP (Vectastain, Burlingame Calif.), and developed using DAB(3,3-diaminobenzidine).
Results
Haired and bald human scalp was procured from individuals undergoing hair transplantation, and representative tissue was examined histologically to confirm the presence of haired and bald scalp (FIG. 1A-B, respectively). Bald scalp exhibited a larger number of miniaturized, vellus-like hair follicles (HF) that satisfied the formal definition of a reduction from the minimum normal hair diameter of 0.08 mm to <0.06 mm. Generally there was a large variation in size of sectioned follicles in the bald scalp. Occasional follicles demonstrated fibroplasia in the inferior portion of the follicle below the hair shaft (called fibrous streamers). There was also a decrease in the normal percentage (>93%) of anagen follicles: In addition, many follicles were found to have inflammatory infiltrates, which were composed of lymphocytes as well as mast cells, and were centered around the lower infundibulum of the HF. Hair follicle miniaturization was confirmed with scanning electron microscopy (SEM), which demonstrated dramatic miniaturization of follicles in the bald scalp (FIG. 1D-E).
None of the subjects showed histopathology consistent with other causes of inflammatory hair loss such as alopecia areata (lymphocytic infiltrates around bulbs of anagen follicles), or cicatricial alopecias (often lichenoid infiltrates associated with complete loss of follicular structures), confirming that the subjects had AGA.
Example 2
Suprabasal Bulge Cells, But Not HF Stem Cells, are Depleted in Bald Scalp  
To determine whether destruction of HF stem cells accounted for follicle miniaturization, immuno-histochemical staining was performed on bald scalp with an antibody to KRT15, a marker for follicle stem cells. KRT15 expression was detected in the miniaturized follicles (FIG. 1C). To determine whether bald scalp exhibited changes in stem cell number, keratinocyte suspensions were stained for KRT15 and FST protein (both intracellularly) and were subjected to flow cytometry to identify bulge follicle stem cells. FST is also a marker for HF stem cells. Cells were also stained for the basal cell marker alpha-6 integrin.
Cells were effectively permeabilized, as evidenced by the staining of over 90% of cells with antibodies against actin, in contrast to minimal staining from an unrelated isotype antibody (FIG. 2A). A high degree of overlap was observed between KRT15 and FST staining, with comparatively fewer single positive cells (KRT15+/FST− or KRT15−/FST+) than double positive or double negative cells (KRT15+/FST+ or KRT15−/FST−) as shown by the slope of the KRT15 vs. FST plot, which was close to 1 (FIG. 2B). Haired samples from each patient yielded similar results.
Percentages of HF stem cells in the basal layer of the bulge, defined as KRT15+ or FST+ and alpha-6 integrin+, were similar between haired and bald scalp for all three paired samples (FIG. 2). The percentage of the KRT15+/alpha-6 integrin+ population in the haired and bald scalp was, respectively, 2.12 vs. 2.39 in the 1st patient (FIG. 2C); 2.05 vs. 2.55 in the 2nd patient. Similarly, in the 3rd patient, the percentage of the FST+/alpha-6 integrin+ populations in haired and bald scalp were 1.52% vs. 1.38% (FIG. 2D). Thus, the number of follicular stem cells in bald versus non-bald scalp was essentially constant.
The KRT15+ and FST+ populations differed between the bald and haired scalp with respect to the distribution of alpha-6 integrin+ cells. Fewer alpha-6 integrin− cells were found in the stem cell compartment of bald scalp. The percentages of the KRT15+/alpha-6 integrin− population were 0.7% and 0.26% in haired and bald scalp, respectively, and the FST+/alpha-6 integrin− population were 0.96% to 0.45%, respectively. Thus the ratio of KRT15+/alpha-6 integrin+ to KRT15+/alpha-6 integrin− increased from 2.17 to 5.3 between haired and bald scalp, and the ratio of FST+/alpha-6 integrin+ to FST+/alpha-6 integrin− cells increased from 2.2 to 5.3. The 3rd subject exhibited a similar 2-fold increase in the ratio of KRT15+/alpha-6 integrin+ to KRT15+/alpha-6 integrin− cells (1.55 vs. 2.97).
Thus, bald scalp exhibited a relative decrease in the proportion of alpha-6 integrin negative cells within the stem cell compartment.
Example 3
Haired and Bald Scalp Exhibit Increased Expression of Organogenesis and Inflammation Genes, Respectively  
To measure differences in stem cell marker expression between AGA and normal samples, RNA was isolated from haired and bald scalp samples from 5 patients, converted to cRNA, and probed with an Affymetrix® U133A chip, testing 22,215 probe sets. 12,786 probe sets were found to be expressed in at least one sample. After intensity readings were obtained for probe sets, geometric means were taken of the patient-specific ratios of haired to bald scalp. Data was analyzed with a 3-way ANOVA algorithm to assign p values (significance), based on the primary variable of haired vs. bald sample, while the variables of patient and date of experiment were considered as secondary random effects. All probe sets tested were then ordered according to p value of the differential expression from most to least significant. The top 251 genes according to statistical significance are depicted in FIG. 3. To corroborate the statistical test used, several additional tests were performed, as described below.
First, correlation within duplicates of haired samples and within duplicates of bald samples was examined. High degrees of correlation were observed (FIG. 4A), indicating the success of the use of internal controls between patients. Secondly, all 10 samples were clustered according to common gene expression in a hierarchical analysis using the 250 most significant genes. Haired specimens and bald samples were each grouped together, providing additional collaborative evidence (FIG. 4B). In addition, this list of genes was compared between patients. Genes that exhibited higher expression in the haired scalp also exhibited a uniform direction of slope among all patients (FIG. 4C, left panel). Similarly, genes higher in the bald scalp exhibited a uniform direction of slope among all patients (FIG. 4C, right panel). Thus, the most statistically significant genes exhibited very uniform behavior across all subjects, further corroborating the gene expression data.
In order to characterize in a non-biased fashion the global expression patterns of genes that were higher in either the donor or recipient, the functional categories of each of the 251 most significantly different genes was determined based on Gene Ontology (GO) classifications (Gene Ontology: The tool for unification of biology. The Gene Ontology Consortium (2000) Nature Genet. 25: 25-29). The 251 most significant genes were subdivided into 2 gene lists: those which were enriched in the haired scalp (169), and those enriched in the bald scalp (81). Each gene list was then analyzed for any common gene functional GO categories within the list using a computerized algorithm. P values were assigned based on the significance of overlapping common categories. Only categories populated by 5 probe sets were considered in the analysis. 13 common categories were found for the genes higher in the donor scalp, and 8 for the bald scalp (FIG. 4D). The 4 most populated gene function categories were development, morphogenesis, organ development and organogenesis, with p values ranging from 1.99×10−10 to 4.72×10−12. In addition to multiple known genes, genes not known to be expressed in HF were discovered.
Thus, findings of the present invention reveal genes that participate in hair growth, healthy HF maintenance and cycling, and hair loss.
Example 4
Novel HF Genes Identified by Analysis of Gene Expression
Next, the fold change magnitude and significance of specific gene changes between haired and bald scalp were characterized. The geometric means from the 5 subjects of the abundance of each transcript were expressed as a fold-change, with a positive value indicating enrichment in the haired scalp, and a negative value indicating enrichment in bald scalp.
Consistent with a lack of gross changes in the cell numbers of the bulge stem cell compartment, no significant differences were observed between the expression of KRT15 and FST (FIG. 5A).
To determine the contribution of UV light to the genetic signature of bald scalp, expression of genes known to be affected by UV light was measured. Collagen message was significantly increased (Col11A1 p=0.0038, Col3A1 p=0.0046, arguing against a role of UV light. Expression of PCNA (p=0.917), CDK4 (p=0.797), and cyclin G (p=0.257), which are induced by UV light, was not significantly changed (FIG. 5A). UV light exposure would also be expected to result in fewer T cells and Langerhans cells. To the contrary, however, evidence of more immune cellular infiltration was detected in the bald scalp. Thus, UV light does not fully explain the genetic signature in the bald scalp.
To test the role of steroid metabolism in aging, the expression of genes involved in steroid metabolism was examined. The androgen receptor (AR) and the enzyme 3-beta-HSD were more abundant in the bald scalp (p<0.05; FIG. 5A), thus corroborating the findings of the present invention.
Immune-related gene expression in bald vs. haired scalp was also examined. Multiple MHC genes, both class I and II, were enriched in the bald scalp (FIGS. 3 and 5A). In addition, transcripts specific for T cells, Langerhans cells and mast cells were enriched in a statistically significant manner in the bald scalp (CD4 [p=0.017], Cd1a [p=0.0087], FCER1A [p=0.046] (FIG. 5A). Several genes not known to be expressed in human HF are depicted in FIG. 5B. These genes also exhibited a high degree of statistical significance (minimum p value=0.001) and minimum fold-enrichment in haired scalp of 4.5.
Example 5
In Situ and Immuno-Histological Characterization of Novel HF Genes
In situ hybridization and immuno-histochemistry was next used to determine tissue patterns of expression of significantly enriched transcripts in the haired scalp, using human haired scalp samples from different patients than those used to generate the array and flow cytometry data.
Microarray showed that LRRC15 was upregulated 4.5 fold in the haired samples (FIG. 5B). LRRC15 is a transmembrane glycoprotein with leucine-rich repeats. To determine whether LRRC15 functions in cell migration, LRRC15 expression was measured in scalp samples by immuno-histochemistry. LRRC15 was present in Huxley's layer and the cuticle layer of the inner root sheath, especially at the lower follicle (FIG. 6A), which is an area of rapid cell movement during hair growth. Thus, LRRC15 functions in cell migration necessary for hair growth.
Serpin A was up-regulated 5.7 fold in the haired samples. Serpin A is, in another embodiment, a Glade A anti-protease in the same family as anti-trypsin and anti-chymotrypsin. Serpin A was expressed in the companion layer of the outer root sheath, as shown by immuno-histochemistry (FIG. 6B).
GPR49 (LGF5, HG38), another leucine rich repeat-containing protein, was upregulated 6.8 fold in the haired samples, and was expressed in human outer root sheath cells, as shown by immuno-histochemistry (FIG. 6C).
GPR49 is known to be upregulated in the mouse bulge (outer root sheath), thus further confirming results of the present invention. Enrichment of this G-protein in anagen/terminal follicles show its utility as a drug target for stimulating hair growth.
The Angiopoietin-like gene CDT6 (upregulated 18 fold in the haired samples) is an anti-vascular factor that is also expressed in the cornea (Corneal Derived Transcript 6), and thought to maintain the avascularity of the cornea. CDT6 was expressed in the outer root sheath, as shown by immuno-histochemistry (FIG. 6D), which is also avascular.
GPRC5D (upregulated 19.5 fold in haired samples) is a homologue of RAIG-1 (retinoic acid inducible gene-1). GPRC5D was expressed in the inner root sheath and precortical cells of the hair, as shown by immuno-histochemistry (FIG. 6E).
FGF18 (upregulated almost 6 fold in the haired samples; FIG. 5B) was found to be expressed in the inner root sheath, the companion layer, and to a lesser extent in the suprabasal outer root sheath of the bulge area (FIG. 6F-G).
The genes identified in this Example are all enriched in haired scalp, and are thus therapeutic targets for stimulating hair growth.
Example 6
PGD2S Levels are Elevated in Bald Scalp Materials and Experimental Methods
Prostaglandin D2 Measurements in Human Scalp
Human scalp was obtained from hair transplants to use in PGD2 measurements. Haired samples were taken from donor sites of hair-bearing scalp from the occiput. Bald samples were taken from recipient tissue taken in the bald frontal scalp to create areas of placement for donor grafts. Immediately post surgery samples were placed at 4 degrees Celsius. Sample weights varied from 0.14-0.94 g. and all results were normalized to starting tissue weight. Within 1-2 days, samples were frozen in liquid nitrogen, and frozen at −70 degrees Celsius for at least 24 hours. After thawing, samples were diluted in 1 ml of acetone and homogenized using a table-top PowerGen 700 Fisher mechanical tissue grinder for 30 seconds at setting 3. Samples were vortexed, centrifuged at 5000×g for 10 minutes, with retention of the supernatant and subsequent second extraction performed on the pellet. Combined supernatants were dried in a speed-vac centrifuge, resuspended in 100 microliter (mcL) of EIA buffer, and PGD2 concentration was determined relative to a prepared standard curve using the Cayman Chemical PGD2-mox ELISA kit. Values were normalized to haired scalp for fold determination.
Results
In addition to the above genes, levels of the lipocalin (brain) isoform prostaglandin D2 synthase (PGD2S) were elevated in bald scalp relative to haired scalp (FIG. 7A). To confirm this finding, PGD2 levels were tested in bald and haired tissue from 3 patients. PGD2 was elevated in all bald samples, at an average fold increase of 11.6 (FIG. 7B). This increase in PGD2 was verified in 1 individual by mass spectrometry. PGD2 was detected as 17 pg/mg of tissue in haired scalp and 75.5 pg/mg in bald scalp, representing a 4.4 fold increase in bald tissue. PGF2a also was slightly elevated in bald scalp with 6.7 pg/mg in haired scalp and 15.9 pg/mg in bald scalp. PGE2, however, exhibited the reverse trend with PGE2 present at 22.7 pg/mg in the haired scalp and 12.0 pg/mg in the bald scalp (FIG. 7C).
The RT-PCR results were further confirmed in 2 individuals not measured in the original array study by quantitative RT PCR, demonstrating a 5.23 and 10.7 fold increase of L-PGDS in bald scalp over haired scalp (FIG. 7D). Immunohistochemical staining of L-PGDS revealed an increase in bald scalp, with L-PGDS appearing in the cells along the fibrous root sheath populated by dermal fibroblasts, as well as in scattered locations intrafollicularly. Given the lack of expression of L-PGDS in hematopoietic cells, increases in L-PGDS were not from the sparse inflammatory cell infiltrate occasionally present in AGA.
Thus, PGD2 synthase and PGD2 are targets for ameliorating common baldness, e.g. AGA.
Example 7
Haired Scalp Contains a Population of CD200High Alpha-6 IntegrinHigh Cells that are Lacking in Bald Scalp
Haired and bald scalp samples from 5 AGA patients were subjected to staining and FACS for alpha-6 integrin and CD200. In each case, haired and bald samples from the same patient were compared. A population of alpha-6 integrinhigh CD200high cells was found to be present in the haired but not bald samples (FIG. 9). These findings demonstrate the existence of a stem cell population that can be transplanted to generate new HF and treat baldness. Further, these findings demonstrate that administration of CD200 and analogues thereof can be used to treat baldness.
Example 8
CD200High Alpha-6 Integrinhigh Cells Possess High In Vitro Proliferative Potential
Materials and Experimental Methods
Equivalent numbers (5,000 cells/flask) of living alpha-6 integrinhigh CD200high cells and mid-follicle cells (positive control) are prepared and seeded into T-25 flasks (Corning) onto a lethally irradiated NIH 3T3 cell feeder layer. Hair follicle cells are cultured in a 3:1 mixture of DMEM and Ham's F12 medium (Invitrogen Corp.) supplemented with 10% FBS, 180 μM adenine, 5 μg/ml insulin, 0.5 μg/ml hydrocortisone, 0.1 nM cholera toxin (Sigma-Aldrich), and 10 ng/ml epidermal growth factor (Invitrogen Corp.) for 2 weeks. Colonies are fixed with paraformaldehyde (Electron Microscopy Sciences) and stained with a 1:1 mixture of Rhodamine B and Nile blue sulfate solution (Sigma-Aldrich), and the number of colonies is per flask is counted.
Results
The proliferative potential of alpha-6 integrinhigh CD200high cells is measured in a colony forming assay. Alpha-6 integrinhigh CD200high cells are found to exhibit high in vitro proliferative potential, consistent with their role as HF stem cells.
Example 9
CD200High Alpha-6 Integrin+ Cells are Able to Reconstitute HF
Materials and Experimental Methods
Alpha-6 integrinhigh CD200high cells expressing a marker protein are isolated by FACS and combined with and equal number of freshly isolated dermal cells from neonatal mice, and injected subcutaneously (sc) (50 mcL of 1×104 cells per mcl suspension) into CB-17 Icr scid/scid mice. In other experiments, cells are placed in tracheas, then implanted sc into the mice. 4 weeks after implantation, mice are sacrificed, and tracheas or subcutaneous growths are removed and assayed for marker protein activity. As negative controls, dermal cells alone or alpha-6 integrinhigh CD200high cells alone are injected. In some experiments, ROSA26 reporter mice, which express beta-galactosidase under control of the ROSA26 promoter, are used as the source of the alpha-6 integrinhigh CD200high cells.
Results
To further characterize the stem cell capacity of alpha-6 integrinhigh CD200high cells, these cells are engineered to express a reporter protein and transplanted into immunodeficient mice. Alpha-6 integrinhigh CD200high cells exhibit stem cell proliferative capacity in this assay.
Example 10
Gene Expression Profile of CD200High Alpha-6 IntegrinHigh Cells
The gene expression profile of alpha-6 integrinhigh CD200high cells is determined, as described in Example 3. Genes enriched in the alpha-6 integrinhigh CD200high cells (e.g. relative to non-bulge basal keratinocytes) play a role in HF formation. Thus, administration of these genes, their protein products, or mimetics and analogues thereof, can be used to stimulate hair growth.
Example 11
PGD2S Levels are Elevated in Skin with Sebaceous Gland Hypertrophy
Sebaceous gland hypertrophy is induced in mice, using a nude mouse grafting model with an altered dermal component, using grafts with an unaltered dermal as a negative control, and PGD2 levels are measured in the both types of skin. PGD2 levels are elevated in the areas of the skin exhibiting sebaceous gland hyperplasia, indicating the role of PGD2 in sebaceous gland hyperplasia, acne, and rosacea.
Example 12
PGD2S Inhibition Ameliorates Sebaceous Gland Hypertrophy
Mice with sebaceous gland hypertrophy are treated with inhibitors of PGD2, and extent of hypertrophy is determined. Sebaceous gland hypertrophy is ameliorated in skin treated with PGD2 inhibitors, but not in untreated areas of the same mice or untreated mice, confirming the role of PGD2 in sebaceous gland hyperplasia, acne, and rosacea.
Example 13
Application of PGD2S Induces Alopecia
Materials and Experimental Methods
25 day-old (corresponding to end of the first telogen stage) wild-type c57/black mice were treated with 200 ng of PGD2 (Cayman Chemicals) diluted in 200 μl of acetone applied to the central back after shaving. Subsequent treatments were performed every 3 days, ending on day 40 For a total of 5 applications. Photographs were taken on day 46 of life, after the end of the 2nd anagen. In the second experiment, mice were shaved and treated with 10 μg of PGD2 dissolved in 200 μl of acetone on days 66 and 69 of life, and then photographed on day #121.
Results
The effect of PGD2 on hair growth was directly determined by applying PGD2 to the backs of mice. Application of PGD2 induced alopecia following the second anagen phase (FIG. 10A). In another experiment, application of PGD2 was shown to prevent hair regrowth over 50 days after the last application (FIG. 10B).
Example 14
K14-COX2 Mice Exhibit PGD2 Elevation and Hair Growth Defects
K14-COX2 mice were examined for hair phenotype. The mice exhibited very low gross hair coat volume, without significant change in hair follicle number and anagen percentage of mice at day 29, demonstrating an early defect in hair growth. Sebaceous hyperplasia was also detected at this age. By day 39, sebaceous glands had enlarged further and many hairs were miniaturized (FIG. 11A).
To verify the contribution of L-PGDS to the phenotype of K14-COX2 mice, immunohistochemistry was performed with antibodies against L-PGDS. Adult mice expressed L-PGDS in an infiltrate of dermal cells, hair sheath fibroblasts, and melanocytes. Staining was detected in anagen dermal papilla in wild-type mice (FIG. 11B).
PGD2 were measured in the K14-COX2 mouse skin via mass spectrometry. PGD2 was 10-fold more abundant than PGE2 in K14-COX2 mice (820.4 ng/gram of tissue vs. 82.4 ng/gram). PGD2 levels were also significantly higher in K14-COX2 mice than in wild type mice in telogen (420.9 ng/gm of tissue, p value=0.01). PGE2 was also elevated, although less so, compared to wild type mice (60.5 ng/gram of tissue). Slight elevations in PGJ-M, a metabolite of PGD2 were also detected (FIG. 12).
These findings corroborate the above androgenetic alopecia findings in humans, by showing that elevations of PGD2 are associated with alopecia and sebaceous gland hyperplasia in mice as well.
Example 15
Identification of Additional Target Genes Regulating Baldness and Hair Loss
The data sets from Example 3 were re-analyzed using the following criteria: Genes exhibiting differential expression patterns in bald vs. non-bald scalp (as described in Example 1) were reviewed to identify cell surface proteins, growth factors, cytokines, G-protein coupled receptors and other signaling molecules, and proteins with biological activities possibly related to miniaturization of the hair follicle in baldness. This additional set of genes is depicted in FIG. 13.

## Claims

1. A method of treating acne or rosacea in a subject, the method comprising the step of contacting said subject with a prostaglandin D2 antagonist, thereby treating acne or rosacea, respectively, in the subject.

2. The method of claim 1, further comprising the step of contacting said subject with a pro-hair growth prostaglandin which is a prostaglandin E1, a prostaglandin E2, a prostaglandin F2a, an analogue thereof or a combination thereof.