# Patent

## Title
Preparation of 2-methyl-4-amino-5(substituted-1H-1,2,3-triazolyl)methylpyrimidine derivatives and microbicidal activity thereof

## Abstract

Disclosed is a derivative of 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazol)methylpyrimidine of general formula I and microbicidal activity thereof. In the formula, R1 represents hydrogen, I; X represents O or NH; Y represents phenyl of substituted phenyl, benzoyl or substituted benzoyl, phenyloxyacetyl or substituted phenyloxyacetyl; the substituents on the phenyl rings which Y involves are: H, halogen, nitro, cyano, CF3, C1 ̃4 alkyl, methoxyl, C1 ̃2 carboxyl or carboxylic ester groups; any position of phenyl rings can be mono- or multi-substituted by identical or different substituents. The compound has a significant inhibition effect on cucumber bacterial angular leaf spot, tomato bacterial leaf spot, cucumber brown blot, cucumber downy mildew, rice sheath blight, Gibberella saubinetii, Alternaria solani, Botrytis cinerea, Alternaria alternate and Colletotrichum orbiculare and it can be used as an effective ingredient of microbicides.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation application of International Patent Application No. PCT/CN2012/081261, filed Sep. 11, 2012, which itself claims the priority to Chinese Patent Application Nos. 201110268908.7 and 201210331267.X, filed Sep. 13, 2011 and Sep. 10, 2012, respectively, in the State Intellectual Property Office of P.R. China, which are hereby incorporated herein in their entireties by reference.
Some references, if any, which may include patents, patent applications and various publications, may be cited and discussed in the description of this invention. The citation and/or discussion of such references, if any, is provided merely to clarify the description of the present invention and is not an admission that any such reference is “prior art” to the invention described herein. All references listed, cited and/or discussed in this specification are incorporated herein by reference in their entireties and to the same extent as if each reference was individually incorporated by reference.


FIELD OF THE INVENTION
The present invention relates to preparation of 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine derivatives and microbicidal activity thereof.
BACKGROUND OF THE INVENTION
Current development and study of new pesticides are focused on searching and discovery of pesticidal active compounds that not only have new structures but also have new targets. In the process of regulation and control of energy metabolism, conversion of pyruvic acid from glycolysis into acetyl CoA as substrate for tricarboxylic acid cycle is a very important stage. This conversion is catalyzed by pyruvic dehydrogenases. If the pyruvate dehydrogenases are inhibited, pyruvic acid would be reduced into lactic acid instead of being converted into acetyl CoA. Accordingly, the source of acetyl CoA required in tricarboxylic acid cycle is limited, resulting in reduced yield of Adenosine Triphosphate (ATP), resulting in energy metabolic disturbance and tissue blocking, and under serious conditions resulting in death. Based on this biochemical feature, the pyruvic dehydrogenase complex may be used as a target of pesticides. Currently, there are reports about inhibitors of pyruvic dehydrogenases. For example, thiamine pyrophosphate analogs A-1 and A-2 are reported as highly active inhibitors against pyruvic dehydrogenases in microbes.




Although the thiamine pyrophosphate analogs A are a group of highly active inhibitors against pyruvic dehydrogenases in microbes, these compounds are complex in structure, difficult in synthesis and have low value in application, especially have not yet shown application value in agriculture. Accordingly, with pyruvic dehydrogenases in microbes as target, novel highly active inhibitors are designed and synthesized by applicant of this invention. Specifically, novel structural compounds are discovered as microbicide useful in agriculture. Those novel compounds are significant and valuable in study and development of novel highly active microbicide.
SUMMARY OF THE INVENTION
In one aspect, the present invention is directed to novel triazolyl methylpyrimidine derivatives. Specifically, the present invention is directed to a novel 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine derivative having microbicidal activity, and preparation and use thereof. The compounds not only have high inhibition on pyruvic dehydrogenases in microbes, but also have superior microbicidal activity.
In one embodiment, the compound of the present invention has a structural of general formula I:




In the formula I, R1 represents hydrogen or iodine (I);
X represents O or NH;
Y represents phenyl or substituted phenyl, benzoyl or substituted benzoyl, phenoxyacetyl or substituted phenoxyacetyl. A substituent on the benzene ring involved in Y is H, halogen, nitro, cyano, CF3, C1-4 alkyl, methoxy, C1-2 carboxy or carboxylate. The substituents on the benzene ring of Y are mono- or multi-substituted at any position of the benzene ring, and the substituents can be the same or different.
The compounds of formula I includes 2-methyl-4-amino-5-((4-substituted phenoxymethyl-5-substituted)-1H-1,2,3-triazol-1-yl)methylpyrimidine compounds represented by general formula I-1;

    N-((1-((2-methyl-4-aminopyrimidin-5-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)substituted benzoyl ester(amine) compounds represented by general formula I-2; and
    N-((1-((2-methyl-4-aminopyrimidin-5-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-2-(substituted phenoxy) acetyl ester(amine) compounds represented by general formula I-3.






The structures of the three new compounds of general formulas I-1, I-2 and I-3 provided by the present invention have not been reported in any patent or literature. The structures of formulas I-1, I-2 and I-3 are defined respectively as follows.
In the 2-methyl-4-amino-5-((4-substituted phenoxymethyl-5-substituted)-1H-1,2,3-triazol-1-yl)methylpyrimidine compounds represented by general formula I-1 above, X is oxygen; R1 is defined as in general formula I; R2 is H, halogen, nitro or C1-4 alkyl; R3 is H, halogen, nitro, cyano, CF3, C1-4 alkyl, methoxy, C1-2 carboxy or carboxylate; and positions of R2 and R3 are exchangeable.
In the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine derivatives represented by general formula I-2 above, X is defined as in general formula I; R1 is defined as in general formula I; R2 is H, halogen or nitro; R3 is H, halogen, nitro or C1-4 alkyl; and positions of R2 and R3 are exchangeable.
In the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine derivatives represented by general formula I-3 above, X is defined as in general formula I; R1 represents hydrogen; R2 is H, halogen, nitro, CF3 or C1-4 alkyl; R3 is H, halogen, nitro, C1-4 alkyl or methoxy; and positions of R2 and R3 are exchangeable.
The compounds of general formula I above according to the present invention have significantly controlling effects on bacterial spot of cucumber, tomato bacterial leaf spot, corynespora leaf spot of cucumber, downy mildew of cucumber, rice sheath blight disease, wheat scab, early blight of tomato, gray mold of cucumber, tobacco brown spot and anthracnose of cucumber, and may be used as microbicide.
Methods for preparing 2-methyl-4-amino-5-((4-substituted phenoxymethyl-5-substituted)-1H-1,2,3-triazol-1-yl)methylpyrimidine compounds represented by general formula I-1: a ring-closing reaction of the compounds of general formula II with substituted phenoxypropyne in the presence of catalyst or catalyst and organic base at temperature of −10° C. to 80° C. for 1-24 hours. Method A:




wherein R1, R2 and R3 are defined as in general formula I-1 above.

In the reaction above, a molar ratio of the compounds of formula II to substituted phenoxypropyne to catalyst to organic base is 1:0.8-1.2:0.01-0.15:2. The organic solvent used as the reaction solvent is dichloromethane, dioxane, dichloroethane, acetone, tert-butyl alcohol:water, benzene, ethyl acetate, tetrahydrofuran, acetonitrile, N,N-dimethyl formamide or dimethyl sulfoxide. The catalyst is CuI, CuBr(PPh3)3, CuSO4.5H2O:sodium ascorbate, CuBr or Cu(OAc)2. The organic base is triethylamine, diisopropylethylamine, pyridine or piperidine.
Method for preparing N-((1-((2-methyl-4-aminopyrimidin-5-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)substituted benzoyl ester(amine) compounds represented by general formula I-2: ring-closing reaction of the compounds of general formula II with substituted benzoyloxy(amino)propyne in the presence of catalyst or catalyst and organic base at temperature of −10° C. to 80° C. and catalytic conditions for 1-24 hours. Method B:




wherein X, R2 and R3 are defined as in general formula I-2 above.

In the reaction above, a molar ratio of the compounds of formula II to substituted benzoyloxypropyne to catalyst to organic base is 1:0.8-1.2:0.01-0.15:2. The organic solvent used as the reaction solvent is dichloromethane, dioxane, dichloroethane, acetone, tert-butyl alcohol:water, benzene, ethyl acetate, tetrahydrofuran, acetonitrile, N,N-dimethyl formamide or dimethyl sulfoxide. The catalyst is CuI, CuBr(PPh3)3, CuSO4.5H2O:sodium ascorbate, CuBr or Cu(OAc)2. The organic base is triethylamine, diisopropylethylamine, pyridine or piperidine.
Method for preparing N-((1-((2-methyl-4-aminopyrimidin-5-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-2-(substituted phenoxy)acetyl ester(amine) compounds represented by general formula I-3: ring-closing reaction of the compounds of general formula II with substituted phenoxyacetoxypropyne in the presence of catalyst or catalyst and organic base at temperature of −10° C. to 80° C. and catalytic conditions for 1-24 hours. Method C:




wherein X, R2 and R3 are defined as in general formula I-3 above.

In the reaction above, a molar ratio of the compounds of formula II to substituted phenoxyacetoxypropyne to catalyst to organic base is 1:0.8-1.2:0.01-0.15:2. The organic solvent used as the reaction solvent is dichloromethane, dioxane, dichloroethane, acetone, tert-butyl alcohol:water, benzene, ethyl acetate, tetrahydrofuran, acetonitrile, N,N-dimethyl formamide or dimethyl sulfoxide. The catalyst is CuI, CuBr(PPh3)3, CuSO4.5H2O:sodium ascorbate, CuBr or Cu(OAc)2. The organic base is triethylamine, diisopropylethylamine, pyridine or piperidine.
These and other aspects of the present invention will become apparent from the following description of the preferred embodiment taken in conjunction with the following drawings, although variations and modifications therein may be effected without departing from the spirit and scope of the novel concepts of the invention.


DETAILED DESCRIPTION OF THE INVENTION
The invention is more particularly described in the following examples that are intended as illustrative only since numerous modifications and variations therein will be apparent to those skilled in the art. Various embodiments of the invention are now described in detail. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The terms used in this specification generally have their ordinary meanings in the art, within the context of the invention, and in the specific context where each term is used. Certain terms that are configured to describe the invention are discussed below, or elsewhere in the specification, to provide additional guidance to the practitioner regarding the description of the invention. For convenience, certain terms may be highlighted, for example using italics and/or quotation marks. The use of highlighting has no influence on the scope and meaning of a term; the scope and meaning of a term is the same, in the same context, whether or not it is highlighted. It will be appreciated that same thing can be said in more than one way. Consequently, alternative language and synonyms may be used for any one or more of the terms discussed herein, nor is any special significance to be placed upon whether or not a term is elaborated or discussed herein. Synonyms for certain terms are provided. A recital of one or more synonyms does not exclude the use of other synonyms. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification.
As used in the description herein and throughout the claims that follow, the meaning of “a”, “an”, and “the” includes plural reference unless the context clearly dictates otherwise.
As used herein, “around”, “about” or “approximately” shall generally mean within 20 percent, preferably within 10 percent, and more preferably within 5 percent of a given value or range. Numerical quantities given herein are approximate, meaning that the term “around”, “about” or “approximately” can be inferred if not expressly stated.
The methods for preparation of the compounds of formula I of the present invention, including the compounds of formula I-1, I-2 and I-3, will be described specifically. The present invention is merely described rather than limited by the embodiments.
Embodiment 1
Preparation of Compound 1




1 mmol 2-methyl-4-amino-5-azido methylpyrimidine and 1 mmol 4-nitrophenoxypropyne were dissolved in a solvent of 6 ml tert-butyl alcohol and water (ratio by volume of tert-butyl alcohol to water=2:1). 0.01 mmol blue vitriol and 0.1 mmol sodium ascorbate were added respectively thereto, and stirred at 60° C.-70° C. for 5-7 hours. After the reaction was completed, 50 ml water was added. With stirring, solid was precipitated out, then the solid precipitant was filtered by suction, and dried, to give the target compound as yellow solid. The yield is 79%, and melting point (mp) is 202-204° C.
Elementary analysis/%:
Calculated value: C, 52.78; H, 4.43; N, 28.73.
Measured value: C, 52.53; H, 4.33; N, 28.22.
1H Nuclear Magnetic Resonance (NMR) (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3); 5.31 (s, 2H, OCH2); 5.46 (s, 2H, CH2); 6.95 (s, 2H, NH2); 7.25-7.26 (d, J=9.0 Hz, 2H, Ar—H); 8.02 (s, 1H, triazole CH); 8.21-8.22 (d, J=9.6 Hz, 2H, Ar—H); 8.27 (s, 1H, pyrimidine CH).
Mass Spectroscopy (MS) (70 eV) m/z (%): 342 (M+1)+.
The compounds 2-22 were prepared similar to the method of preparing compound 1. The structural data are identified as follows.
Compound 2




The pure product as white solid was obtained with yield of 78%, and m.p. is 168-170° C.
Elementary analysis/%:
Calculated value: C, 54.47; H, 4.57; N, 25.41.
Measured value: C, 54.74; H, 4.43; N, 25.17.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.13 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.05-7.06 (d, 2H, J=9.0 Hz, Ar—H), 7.32-7.34 (d, 2H, J=9.6 Hz, Ar—H), 8.01 (s, 1H, triazole CH), 8.21 (s, 1H, pyrimidine CH).
Compound 3




The pure product as white solid was obtained with yield of 94%, and m.p. is 190-192° C.
Elementary analysis/%:
Calculated value: C, 58.69; H, 5.47; N, 22.81.
Measured value: C, 58.88; H, 5.49; N, 22.62.
1H NMR (600 MHz, DMSO-d6): δ 1.28-1.31 (t, 3H, J=10.8 Hz, CH3), 2.30 (s, 3H, CH3), 4.24-4.29 (q, 2H, J=10.8 Hz, CH2), 5.22 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.12-7.14 (d, 2H, J=12.6 Hz, Ar—H), 7.89-7.91 (d, 2H, J=12.6 Hz, Ar—H), 8.01 (s, 1H, triazole CH), 8.21 (s, 1H, pyrimidine CH).
ESI-MS m/z: 369 (M+1)+.
Compound 4




The pure product as white solid was obtained with yield of 89%, and m.p. is 160-161° C.
Elementary analysis/%:
Calculated value: C, 52.75; H, 4.15; N, 23.07.
Measured value: C, 52.46; H, 4.35; N, 22.94.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.23 (s, 2H, OCH2), 5.46 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.30-7.31 (d, 1H, J=7.8 Hz, Ar—H), 7.34-7.36 (t, 2H, J=8.4 Hz, 12.0 Hz, Ar—H), 7.52-7.53 (d, 1H, J=7.8 Hz, Ar—H), 8.02 (s, 1H, triazole CH), 8.24 (d, 1H, pyrimidine CH).
Compound 5




The pure product as white solid was obtained with yield of 80%, and m.p. is 184-185° C.
Elementary analysis/%:
Calculated value: C, 55.73; H, 4.97; N, 24.37.
Measured value: C, 55.41; H, 4.99; N, 24.44.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 2.31 (s, 3H, CH3), 5.20-5.21 (d, 2H, J=5.4 Hz, OCH2), 5.46-5.47 (d, 2H, J=6.0 Hz, CH2), 6.79 (s, 1H, Ar—H), 6.96 (s, 2H, NH2), 7.17-7.18 (d, 1H, J=5.4 Hz, Ar—H), 7.27-7.29 (d, 1H, J=7.2 Hz, Ar—H), 8.03 (s, 1H, triazole CH), 8.25 (s, 1H, pyrimidine CH).
Compound 6




The pure product as white solid was obtained with yield of 78%, and m.p. is 169-171° C.
Elementary analysis/%:
Calculated value: C, 58.69; H, 5.47; N, 22.81.
Measure value: C, 58.88; H, 5.49; N, 22.62.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.21 (s, 2H, OCH2), 5.46 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.18-7.21 (dd, 1H, J=6.6 Hz, 7.8 Hz, Ar—H), 7.35-7.38 (m, 1H, Ar—H), 7.43-7.44 (d, 1H, J=6.0 Hz, Ar—H), 8.01 (s, 1H, triazole CH), 8.23 (s, 1H, pyrimidine CH).
ESI-MS m/z: 349 (M+1)+.
Compound 7




The pure product as white solid was obtained with yield of 76%, and m.p. >260° C.
Elementary analysis/%:
Calculated value: C, 56.47; H, 4.74; N, 24.69.
Measured value: C, 56.35; H, 4.74; N, 24.45.
1H NMR (600 MHz, DMSO-d6): δ 2.34 (s, 3H, CH3), 5.22 (s, 2H, OCH2), 5.47 (s, 2H, CH2), 7.11-7.13 (d, 2H, J=13.2 Hz, Ar—H), 7.20 (s, 2H, NH2), 7.88-7.90 (d, 2H, J=12.6 Hz, Ar—H), 8.06 (s, 1H, triazole CH), 8.26 (s, 1H, pyrimidine CH).
ESI-MS m/z: 341 (M+1)+.
Compound 8




The pure product as yellow solid was obtained with yield of 50%, and m.p. is 210-211° C.
Elementary analysis/%:
Calculated value: C, 46.63; H, 3.65; N, 29.01.
Measured value: C, 46.45; H, 3.86; N, 28.81.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.47 (s, 2H, OCH2), 5.52 (s, 2H, CH2), 6.98 (s, 2H, NH2), 7.80-7.83 (d, 1H, J=14.4 Hz, Ar—H), 8.27 (s, 1H, pyrimidine CH), 8.51-8.54 (m, 1H, Ar—H), 8.75-8.75 (d, 1H, J=4.2 Hz, Ar—H).
13C NMR (DMSO-d6, 150 MHz): δ 25.2, 46.9, 63.6, 108.5, 116.2, 121.2, 125.3, 129.2, 138.7, 139.9, 141.0, 155.2, 155.9, 161.6, 167.3.
ESI-MS m/z: 387 (M+1)+.
Compound 9




The pure product as white solid was obtained with yield of 85%, and m.p. is 142-143° C.
Elementary analysis/%:
Calculated value: C, 58.69; H, 5.47; N, 22.81.
Measured value: C, 58.77; H, 5.48; N, 22.67.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 3.59 (s, 5H, CH2+CH3), 5.10 (s, 2H, OCH2), 5.44 (s, 2H, CH2), 6.95 (s, 2H, NH2), 6.96-6.97 (d, 2H, J=8.4 Hz, Ar—H), 7.16-7.18 (d, 2H, J=9.0 Hz, Ar—H), 8.01 (s, 1H, triazole CH), 8.20 (d, 1H, pyrimidine CH).
13C NMR (DMSO-d6, 150 MHz): δ 25.2, 39.9, 46.7, 51.6, 61.0, 108.2, 114.4, 114.6, 124.5, 126.6, 130.9, 142.8, 156.9, 161.5, 167.0, 171.8.
ESI-MS m/z: 369 (M+1)+.
Compound 10




The pure product as white solid was obtained with yield of 70%, and m.p. is 175-177° C.
Elementary analysis/%:
Calculated value: C, 58.69; H, 5.47; N, 22.81.
Measured value: C, 58.77; H, 5.48; N, 22.67.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.24 (s, 2H, OCH2), 5.46 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.38 (s, 2H, Ar—H), 7.57 (s, 1H, Ar—H), 8.01 (s, 1H, triazole CH), 8.25 (s, 1H, pyrimidine CH).
ESI-MS m/z: 365.22 (M+1)+.
Compound 11




The pure product as yellow solid was obtained with yield of 74%, and m.p. is 142-143° C.
Elementary analysis/%:
Calculated value: C, 55.73; H, 4.97; N, 24.37.
Measured value: C, 55.63; H, 4.86; N, 24.39.
1H NMR (600 MHz, DMSO-d6): δ 2.28 (s, 3H, CH3), 2.31 (s, 3H, CH3), 5.11 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.95 (s, 2H, NH2), 6.89 (s, 1H, Ar—H), 7.03-7.04 (d, 2H, J=3.6 Hz, Ar—H), 7.28-7.31 (d, 2H, J=14.4 Hz, Ar—H), 8.00 (s, 1H, triazole CH), 8.20 (s, 1H, pyrimidine CH).
Compound 12




The pure product as white solid was obtained with yield of 97%, and m.p. is 132-133° C.
Elementary analysis/%:
Calculated value: C, 60.80; H, 5.44; N, 28.36.
Measured value: C, 60.62; H, 5.35; N, 28.60.
1H NMR (600 MHz, CDCl3): δ 2.50 (s, 3H, CH3), 5.18 (s, 2H, OCH2), 5.36 (s, 2H, CH2), 5.59 (s, 2H, NH2), 6.95-6.99 (m, 3H, Ar—H), 7.27-7.30 (t, J=7.8 Hz, Ar—H), 7.62 (s, 1H, triazole CH), 8.19 (s, 1H, pyrimidine CH).
ESI-MS m/z: 297 (M+1)+.
Compound 13




The pure product as white solid was obtained with yield of 87%, and m.p. is 115-117° C.
Elementary analysis/%:
Calculated value: C, 58.88; H, 5.56; N, 25.75.
Measured value: C, 58.97; H, 5.86; N, 25.45.
1H NMR (600 MHz, CDCl3): δ 2.50 (s, 3H, CH3), 3.77 (s, 3H, CH3), 5.13 (s, 2H, OCH2), 5.36 (s, 2H, CH2), 5.60 (s, 2H, NH2), 6.82-6.83 (d, 2H, J=9.0 Hz, Ar—H), 6.89-6.90 (d, 2H, J=8.4 Hz, Ar—H), 7.61 (s, 1H, triazole CH), 8.19 (d, 1H, pyrimidine CH).
ESI-MS m/z: 327 (M+1)+.
Compound 14




The pure product as white solid was obtained with yield of 50%, and m.p. is 202-204° C.
Elementary analysis/%:
Calculated value: C, 62.95; H, 6.21; N, 25.91.
Measured value: C, 62.55; H, 6.37; N, 26.00.
1H NMR (600 MHz, DMSO-d6): δ 2.03 (s, 3H, 2-phenyl-CH3), 2.20 (s, 3H, 3-phenyl-CH3), 2.31 (s, 3H, CH3), 5.10 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.76-6.78 (d, 1H, J=7.2 Hz, Ar—H), 6.95-6.96 (d, 2H, J=7.8 Hz, Ar—H), 7.02-7.04 (t, 1H, J=7.8 Hz, Ar—H), 7.99 (s, 1H, triazole CH), 8.21 (s, 1H, pyrimidine CH).
ESI-MS m/z: 325 (M+1)+.
Compound 15




The pure product as yellow solid was obtained with yield of 81%, and m.p. is 194-196° C.
Elementary analysis/%:
Calculated value: C, 47.94; H, 3.76; N, 26.09.
Measured value: C, 47.59; H, 3.74; N, 22.82.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.31 (s, 2H, OCH2), 5.46 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.25-7.26 (d, 2H, J=9.0 Hz, Ar—H), 8.02 (s, 1H, triazole CH), 8.21-8.22 (d, 2H, J=9.6 Hz, Ar—H), 8.27 (s, 1H, pyrimidine CH).
ESI-MS m/z: 376 (M+1)+.
Compound 16




The pure product as yellow solid was obtained with yield of 90%, and m.p. is 184-185° C.
Elementary analysis/%:
Calculated value: C, 52.78; H, 4.43; N, 28.73.
Measured value: C, 52.64; H, 4.36; N, 28.31.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.34 (s, 2H, OCH2), 5.46 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.13-7.15 (dd, 1H, J=7.2 Hz, 7.8 Hz, Ar—H), 7.57-7.58 (d, 1H, J=8.4 Hz, Ar—H), 7.65-7.68 (d, 1H, J=7.8 Hz, Ar—H), 7.85-7.87 (d, 1H, J=7.8 Hz, Ar—H), 8.01 (s, 1H, triazole CH), 8.23 (s, 1H, pyrimidine CH).
Compound 17




The pure product as white solid was obtained with yield of 73%, and m.p. is 157-158° C.
Elementary analysis/%:
Calculated value: C, 61.92; H, 5.85; N, 27.08.
Measured value: C, 61.74; H, 5.76; N, 27.32.
1H NMR (600 MHz, CDCl3): δ 2.29 (s, 3H, CH3), 2.51 (s, 3H, CH3), 5.16 (s, 2H, CH2), 5.36 (s, 2H, CH2), 5.58 (s, 2H, NH2), 6.85-6.86 (d, 2H, J=8.4 Hz, Ar—H), 7.08-7.09 (d, 2H, J=8.4 Hz, Ar—H), 7.62 (s, 1H, triazole CH), 8.19 (s, 1H, pyrimidine CH).
Compound 18




The pure product as yellow solid was obtained with yield of 45%, and m.p. is 153-154° C.
Elementary analysis/%:
Calculated value: C, 61.92; H, 5.85; N, 27.08.
Measured value: C, 61.81; H, 5.91; N, 28.22.
1H NMR (600 MHz, CDCl3): δ 2.21 (s, 3H, CH3), 2.51 (s, 3H, CH3), 5.19 (s, 2H, CH2), 5.37 (s, 2H, CH2), 5.62 (s, 2H, NH2), 6.88-6.92 (m, 2H, Ar—H), 7.14-7.17 (t, 2H, J=7.8, 8.4 Hz, Ar—H), 7.61 (s, 1H, triazole CH), 8.20 (s, 1H, pyrimidine CH).
Compound 19




The pure product as white solid was obtained with yield of 73%, and m.p. is 58-59° C.
Elementary analysis/%:
Calculated value: C, 61.92; H, 5.85; N, 27.08.
Measured value: C, 61.77; H, 5.67; N, 26.92.
1H NMR (600 MHz, CDCl3): δ 2.32 (s, 3H, CH3), 2.50 (s, 3H, CH3), 5.17 (s, 2H, CH2), 5.36 (s, 2H, CH2), 5.61 (s, 2H, NH2), 6.76-6.80 (m, 3H, ArH), 7.16-7.18 (t, 1H, J=7.8, 7.8 Hz, ArH), 7.63 (s, 1H, triazole CH), 8.19 (s, 1H, pyrimidine CH).
Compound 20




The pure product as white solid was obtained with yield of 63%, and m.p. is 178-179° C.
Elementary analysis/%:
Calculated value: C, 57.32; H, 4.81; N, 26.74.
Measured value: C, 57.21; H, 4.67; N, 26.55.
1H NMR (600 MHz, CDCl3): δ 2.50 (s, 3H, CH3), 5.14 (s, 2H, CH2), 5.37 (s, 2H, CH2), 5.63 (s, 2H, NH2), 6.89-6.91 (m, 2H, Ar—H), 6.96-6.99 (t, 2H, J=8.4, 8.4 Hz, Ar—H), 7.63 (s, 1H, triazole CH), 8.20 (s, 1H, pyrimidine CH).
Compound 21




The pure product as yellow solid was obtained with yield of 74%, and m.p. is 167-168° C.
Elementary analysis/%:
Calculated value: C, 48.01; H, 4.03; N, 22.40.
Measured value: C, 47.87; H, 4.32; N, 22.21.
1H NMR (600 MHz, CDCl3): δ 2.50 (s, 3H, CH3), 5.15 (s, 2H, CH2), 5.36 (s, 2H, CH2), 5.59 (s, 2H, NH2), 6.84-6.85 (d, 2H, J=9.0 Hz, Ar—H), 7.37-7.38 (d, 2H, J=8.4 Hz, Ar—H), 7.62 (s, 1H, triazole CH), 8.19 (s, 1H, pyrimidine CH).
Compound 22




The pure product as white solid was obtained with yield of 62%, and m.p. is 185-186° C.
Elementary analysis/%:
Calculated value: C, 59.80; H, 4.71; N, 30.51.
Measured value: C, 59.77; H, 4.66; N, 30.42.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.32 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.22-7.23 (d, 2H, J=8.4 Hz, Ar—H), 7.79-7.80 (d, 2H, J=8.4 Hz, Ar—H) 8.02 (s, 1H, triazole CH), 8.27 (s, 1H, pyrimidine CH).
Embodiment 2




Preparation of Compound 23
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol iodo-4-nitrophenoxypropyne were dissolved in 5 ml anhydrous tetrahydrofuran. 0.05 mmol CuI and 2 mmol triethylamine were added respectively thereto, and stirred for 12 hours at 40-50° C. Upon addition of water at stirring, solid was precipitated out. The solid precipitant was filtered by suction, and dried to give the yellow solid, with yield of 75%, and m.p. is 127-129° C.
Elementary analysis/%:
Calculated value: C, 42.67; H, 3.58; N, 19.90.
Measured value: C, 42.89; H, 3.59; N, 19.95.
1H NMR (600 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 5.06 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.97 (s, 2H, NH2), 7.05 (s, 3H, Ar—H), 7.31 (s, 2H, Ar—H), 7.69 (s, 1H, pyrimidine CH).
13C NMR (DMSO-d6, 125 MHz): δ 25.27, 48.01, 61.37, 86.15, 107.49, 114.80, 121.16, 129.58, 147.38, 155.09, 158.16, 161.41, 166.86.
ESI-MS m/z: 423 (M+1)+.
The compounds 24-31 and 80-87 were prepared in analogy to the compound 23, with structural data identified as follows.
Compound 24




The pure product as yellow solid was obtained with yield of 74%, and m.p. is 215-217° C.
Elementary analysis/%:
Calculated value: C, 38.56; H, 3.02; N, 20.99.
Measured value: C, 38.26; H, 3.00; N, 21.35.
1H NMR (600 MHz, DMSO-d6): δ 2.34 (s, 3H, CH3), 5.26 (s, 2H, CH2), 5.49 (s, 2H, CH2), 6.98 (s, 2H, NH2), 7.26-7.29 (d, 2H, J=7.8 Hz, Ar—H), 8.22-8.24 (d, 2H, J=9.0 Hz, Ar—H), 8.27 (s, 1H, pyrimidine CH).
ESI-MS m/z (%): 468 (M+1)+.
Compound 25




The pure product as yellow solid was obtained with yield of 74%, and m.p. is 215-217° C.
Elementary analysis/%:
Calculated value: C, 40.83; H, 3.43; N, 17.85.
Measured value: C, 40.58; H, 3.26; N, 17.49.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 2.33 (s, 3H, CH3), 5.05 (s, 2H, CH2), 5.47 (s, 2H, CH2), 6.90-6.92 (m, 3H), 7.06-7.08 (d, 1H, J=2.4 Hz, Ar—H), 7.30-7.32 (d, 1H, J=9.0 Hz, Ar—H).
Compound 26




The pure product as yellow solid was obtained with yield of 83%, and m.p. is 177-179° C.
Elementary analysis/%:
Calculated value: C, 42.49; H, 3.79; N, 18.58.
Measured value: C, 42.45; H, 3.86; N, 18.26.
1H NMR (600 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 3.70 (s, 3H, OCH3), 4.99 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.85-6.88 (t, 3H, J=6.6 Hz, 8.4 Hz), 6.88-6.91 (d, 1H, J=8.4 Hz), 6.92 (s, 1H), 6.96-6.98 (d, 1H, J=9.0 Hz).
Compound 27




The pure product as yellow solid was obtained with yield of 94%, and m.p. is 187-189° C.
Elementary analysis/%:
Calculated value: C, 37.96; H, 2.76; N, 17.71.
Measured value: C, 38.00; H, 2.88; N, 17.44. 1H NMR (600 MHz, DMSO-d6): δ 2.33 (s, 3H, CH3), 5.15 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.90 (s, 2H, NH2), 7.21 (s, 1H, Ar—H), 7.36 (s, 1H, Ar—H), 7.43-7.44 (d, 1H, J=5.4 Hz, Ar—H), 7.68 (s, 1H).
Compound 28




The pure product as yellow solid was obtained with yield of 87%, and m.p. is 183-185° C.
Elementary analysis/%:
Calculated value: C, 39.45; H, 3.09; N, 18.40.
Measured value: C, 39.58; H, 3.12; N, 18.58.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.12 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.94 (s, 2H, NH2), 6.99-7.08 (m, 2H, Ar—H), 7.34-7.35 (d, 3H, J=9.0 Hz, Ar—H).
Compound 29




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 163-165° C.
Elementary analysis/%:
Calculated value: C, 40.83; H, 3.43; N, 17.85.
Measured value: C, 41.18; H, 3.83; N, 17.67.
1H NMR (600 MHz, CDCl3): δ 2.21 (s, 3H, CH3), 2.51 (s, 3H, CH3), 5.08 (s, 2H, CH2), 5.42 (s, 2H, CH2), 5.77 (s, 2H, NH2), 6.91-6.93 (d, 1H, J=9.6 Hz, Ar—H), 7.10 (s, 2H, Ar—H), 8.42 (s, 1H, pyrimidine CH).
Compound 30




The pure product as yellow solid was obtained with yield of 85%, and m.p. is 223-225° C.
Elementary analysis/%:
Calculated value: C, 40.83; H, 3.43; N, 17.85.
Measured value: C, 41.18; H, 3.83; N, 17.67.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.36 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.91 (s, 2H, NH2), 7.60-7.61 (d, 1H, J=8.4 Hz, Ar—H), 8.24-8.25 (d, 1H, J=7.8 Hz, Ar—H), 8.30 (s, 1H, Ar—H).
13C NMR (125 Mz, DMSO-d6): δ 25.28, 48.09, 63.39, 86.83, 114.11, 122.14, 124.59, 125.61, 141.12, 146.23, 155.15, 158.61, 161.45.
Compound 31




The pure product as yellow solid was obtained with yield of 88%, and m.p. is 199-201° C.
Elementary analysis/%:
Calculated value: C, 42.97; H, 3.16; N, 21.92.
Measured value: C, 42.64; H, 3.31; N, 21.68.
1H NMR (600 MHz, DMSO-d6): δ 2.35 (s, 3H, CH3), 5.18 (s, 2H, CH2), 5.46 (s, 2H, CH2), 7.06 (s, 2H, NH2), 7.22-7.23 (d, 2H, J=8.4 Hz, Ar—H), 7.78-7.79 (d, 2H, J=8.4 Hz, Ar—H).
13C NMR (125 Mz, DMSO-d6): δ 25.69, 47.93, 61.88, 86.61, 103.40, 115.88, 119.11, 134.26, 124.64, 146.69, 154.57, 161.24, 161.52.
Compound 80




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 178-180° C.
Elementary analysis/%:
Calculated value: C, 38.56; H, 3.02; N, 20.99.
Measured value: C, 38.26; H, 3.42; N, 20.67.
1H NMR (400 MHz, DMSO-d6): δ 2.34 (s, 3H, CH3), 5.29 (s, 2H, CH2), 5.46 (s, 2H, CH2), 6.97 (s, 2H, NH2), 7.16 (s, 1H, Ar—H), 7.60 (s, 1H, Ar—H), 7.68 (s, 1H, Ar—H), 7.85 (s, 1H, Ar—H).
E-MS (m/z, %): 467.25 (M+, 0.45).
Compound 81




The pure product as yellow solid was obtained with yield of 65%, and m.p. is 146-148° C.
Elementary analysis/%:
Calculated value: C, 39.20; H, 2.88; N, 17.14.
Measured value: C, 39.02; H, 2.53; N, 16.85.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 5.17 (s, 2H, CH2), 5.46 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.31-7.39 (m, 3H, Ar—H), 7.54 (t, 1H, J=8.0 Hz, Ar—H).
E-MS (m/z, %): 490.47 (M+, 1.00).
Compound 82




The pure product as yellow solid was obtained with yield of 67%, and m.p. is 160-162° C.
Elementary analysis/%:
Calculated value: C, 40.93; H, 3.21; N, 19.09.
Measured value: C, 40.71; H, 3.15; N, 19.37.
1H NMR (600 MHz, CDCl3): δ 2.51 (s, 3H, CH3), 5.05 (s, 2H, CH2), 5.42 (s, 2H, CH2), 5.76 (s, 2H, NH2), 6.96-6.99 (m, 4H, Ar—H), 8.42 (s, 1H, CH).
E-MS (m/z, %): 440.94 (M++1, 2.23), 440.20 (M+, 3.38).
Compound 83




The pure product as yellow solid was obtained with yield of 84%, and m.p. is 176-177° C.
Elementary analysis/%:
Calculated value: C, 36.68; H, 2.67; N, 17.11.
Measured value: C, 36.39; H, 2.34; N, 16.96.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.17 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.92 (s, 2H, NH2), 7.239 (s, 2H, Ar—H), 7.57 (s, 1H, Ar—H).
E-MS (m/z, %): 491.34 (M+, 0.69).
Compound 84




The pure product as yellow solid was obtained with yield of 75%, and m.p. is 138-140° C.
Elementary analysis/%:
Calculated value: C, 35.95; H, 2.82; N, 16.77.
Measured value: C, 35.53; H, 3.30; N, 16.80.
1H NMR (400 MHz, DMSO-d6): δ 2.33 (s, 3H, CH3), 5.07 (s, 2H, CH2), 5.47 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.03 (d, 2H, J=8.4 Hz, Ar—H), 7.47 (d, 2H, J=8.0 Hz, Ar—H), 8.22 (s, 1H, CH).
E-MS (m/z, %): 502.03 (M++2, 0.76), 499.91 (M+, 1.17).
Compound 85




The pure product as yellow solid was obtained with yield of 77%, and m.p. is 145-147° C.
Elementary analysis/%:
Calculated value: C, 35.95; H, 2.82; N, 16.77.
Measured value: C, 35.61; H, 3.01; N, 16.52.
1H NMR (600 MHz, CDCl3): δ 2.51 (s, 3H, CH3), 5.18 (s, 2H, CH2), 5.41 (s, 2H, CH2), 5.77 (s, 2H, NH2), 6.86-6.91 (m, 2H, Ar—H), 7.52-7.56 (m, 2H, Ar—H), 8.44 (s, 1H, CH).
E-MS (m/z, %): 499.95 (M+, 0.65).
Compound 86




The pure product as yellow solid was obtained with yield of 88%, and m.p. is 168-170° C.
Elementary analysis/%:
Calculated value: C, 39.45; H, 3.09; N, 18.40.
Measured value: C, 39.46; H, 3.43; N, 18.55.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 5.06 (s, 2H, CH2), 5.46 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.08 (d, 2H, J=8.8 Hz, Ar—H), 7.34 (d, 2H, J=8.8 Hz, Ar—H).
E-MS (m/z, %): 458.27 (M++2, 0.52), 456.27 (M+, 0.79).
Compound 87




The pure product as yellow solid was obtained with yield of 80%, and m.p. is 146-147° C.
Elementary analysis/%:
Calculated value: C, 43.74; H, 3.87; N, 17.00.
Measured value: C, 43.62; H, 4.16; N, 16.72.
1H NMR (400 MHz, DMSO-d6): δ 1.30 (t, 3H, J=6.8 Hz, CH3), 2.32 (s, 3H, CH3), 4.26 (q, 2H, J=7.6 Hz, CH2), 5.16 (s, 2H, CH2), 5.46 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.16 (d, 2H, J=7.6 Hz, Ar—H), 7.92 (d, 2H, J=8.0 Hz, Ar—H).
E-MS (m/z, %): 494.04 (M+, 0.91).
Embodiment 3




Preparation of Compound 32
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol benzoyl propyne ester were dissolved in a solvent of 6 ml DMF. 0.01 mmol blue vitriol and 0.1 mmol sodium ascorbate were added respectively thereto, stirred at 0-10° C. for 5-7 hours. After the reaction was completed, 50 ml water was added. With stirring, solid precipitated out. The solid precipitant was filtered by suction, and was dried, to give the target compound as white solid, yield 88%, and m.p. is 151-153° C.
Elementary analysis/%:
Calculated value: C, 52.03; H, 4.09; N, 26.55.
Measured value: C, 52.44; H, 4.35; N, 26.13.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.44 (s, 2H, CH2), 5.45 (s, 2H, OCH2), 6.96 (s, 2H, NH2), 8.02 ((s, 1H, triazole CH), 8.16-8.17 (dd, 2H, J=2.4 Hz, J=6.6 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.33-8.34 (dd, 2H, J=1.2 Hz, J=7.2 Hz, Ar—H).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.3, 47.0, 58.2, 108.6, 125.5, 127.5, 129.0, 132.0, 138.5, 141.4, 156.8, 160.9, 164.0, 167.6.
The compounds 33-42 are prepared in analogy to the compound 32, with structural data identified as follows.
Compound 33




The pure product as white solid was obtained with yield of 54%, and m.p. is 188-189° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.93; H, 4.15; N, 22.98.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.37 (s, 2H, CH2), 5.44 (s, 2H, OCH2), 6.96 (s, 2H, NH2), 7.59-7.60 (d, 2H, J=7.2 Hz, Ar—H), 7.94-7.95 (d, 2H, J=7.2 Hz, Ar—H), 8.02 ((s, 1H, triazole CH), 8.25 (s, 1H, pyrimidine CH).




Compound 34
The pure product as white solid was obtained with yield of 88%, and m.p. is 198-199° C.
Elementary analysis/%:
Calculated value: C, 56.14; H, 4.42; N, 24.55.
Measured value: C, 56.44; H, 4.42; N, 24.73.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.34 (s, 2H, CH2), 5.44 (s, 2H, OCH2), 6.95 (s, 2H, NH2), 7.34 (s, 2H, Ar—H), 8.01 (s, 2H, Ar—H), 8.01 (s, 1H, triazole CH), 8.28 (s, 1H, pyrimidine CH).
Compound 35




The pure product as white solid was obtained with yield of 75%, and m.p. is 142-143° C.
Elementary analysis/%:
Calculated value: C, 46.38; H, 3.41; N, 27.04.
Measured value: C, 46.33; H, 2.92; N, 27.14.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.46 (s, 2H, CH2), 5.51 (s, 2H, OCH2), 6.96 (s, 2H, NH2), 8.02 ((s, 1H, triazole CH), 8.28 (s, 1H, pyrimidine CH), 8.87 (s, 2H, Ar—H), 9.03 (s, 1H, Ar—H).
Compound 36




The pure product as white solid was obtained with yield of 82%, and m.p. is 85-87° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.17; H, 4.78; N, 23.43.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.38 (s, 2H, CH2), 5.45 (s, 2H, OCH2), 6.95 (s, 2H, NH2), 7.45 (s, 1H, Ar—H), 7.58 (s, 2H, Ar—H), 7.76-7.77 (d, 1H, J=5.4 Hz, Ar—H), 8.00 (s, 1H, triazole CH), 8.28 (s, 1H, pyrimidine CH).
Compound 37




The pure product as white solid was obtained with yield of 90%, and m.p. is 124-125° C.
Elementary analysis/%:
Calculated value: C, 56.14; H, 4.42; N, 24.55.
Measured value: C, 56.26; H, 4.02; N, 24.12.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.39 (s, 2H, CH2), 5.46 (s, 2H, OCH2), 6.96 (s, 2H, NH2), 7.34-7.35 (d, 2H, J=7.8 Hz, Ar—H), 7.69 (s, 1H, Ar—H), 7.87-7.88 (d, 1H, J=6.6 Hz, Ar—H), 8.03 (s, 1H, triazole CH), 8.26 (s, 1H, pyrimidine CH).
Compound 38




The pure product as white solid was obtained with yield of 77%, and m.p. is 202-203° C.
Elementary analysis/%:
Calculated value: C, 60.34; H, 5.36; N, 24.84.
Measured value: C, 60.42; H, 5.38; N, 24.73.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 2.36 (s, 3H, CH3), 5.35 (s, 2H, CH2), 5.44 (s, 2H, OCH2), 6.95 (s, 2H, NH2), 7.31-7.33 (d, 2H, J=8.4 Hz, Ar—H), 7.82-7.84 (d, 2H, J=7.8 Hz, Ar—H), 8.01 ((s, 1H, triazole CH), 8.24 (s, 1H, pyrimidine CH).
Compound 39




The pure product as white solid was obtained with yield of 83%, and m.p. is 165-166° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.04; H, 4.19; N, 23.49.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.44 (s, 2H, OCH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 8.02 (s, 1H, triazole CH), 8.16-8.17 (t, 2H, J=2.4 Hz, J=6.6 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.33-8.34 (t, 2H, J=1.2 Hz, J=7.2 Hz, Ar—H).
Compound 40




The pure product as green solid was obtained with yield of 60%, and m.p. is 174-176° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.04; H, 4.19; N, 23.49.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.39 (s, 2H, OCH2), 5.47 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.48 (s, 2H, Ar—H), 7.73 (s, 2H, Ar—H), 8.25 (s, 1H).
Compound 41




The pure product as green solid was obtained with yield of 69%, and m.p. is 159-160° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.04; H, 4.19; N, 23.49.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 5.45 (s, 2H, OCH2), 5.48 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.99 (s, 1H), 8.16-8.17 (m, 3H, Ar—H), 8.28 (s, 1H, pyrimidine CH).
Compound 42




The pure product as green solid was obtained with yield of 59%, and m.p. is 129-130° C.
Elementary analysis/%:
Calculated value: C, 53.56; H, 4.21; N, 23.42.
Measured value: C, 53.04; H, 4.19; N, 23.49.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 5.45 (s, 2H, OCH2), 5.49 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.99-8.02 (d, 1H, J=12.6 Hz), 8.25-8.26 (d, 2H, J=5.4 Hz), 8.41 (s, 1H, pyrimidine CH).
Embodiment 4




Preparation of Compound 43
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol iodo-4-nitrophenoxypropyne were dissolved in 5 ml anhydrous tetrahydrofuran. 0.05 mmol CuBr and 2 mmol triethylamine were added respectively thereto, and stirred for 12 hours at 50-60° C. Upon addition of water at stirring, the solid precipitated out. The solid precipitant was filtered by suction, and dried to give the green solid, with yield of 78%, and m.p. is 197-199° C.
Elementary analysis/%:
Calculated value: C, 59.43; H, 4.95; N, 30.32.
Measured value: C, 59.19; H, 5.25; N, 30.75.
1H NMR (600 MHz, DMSO-d6): δ 2.28 (s, 3H, CH3), 4.47-4.48 (d, 2H, J=5.4, CH2), 5.38 (s, 2H, CH2), 6.89 (s, 2H, NH2), 7.43-7.46 (dd, 2H, J=7.8, 7.2 Hz, Ar—H), 7.50-7.51 (d, 1H, J=7.8 Hz, Ar—H), 7.84-7.85 (d, 2H, J=7.2 Hz, Ar—H), 7.98 (s, 2H, CH), 9.00 (s, 1H, NH).
IR(KBr) υ (cm−1): 3440 (—NH2), 2960 (—CH3), 2920 (—CH2), 1640 (—C═O), 1500 (—Ar).
The compounds 44-53 are prepared in analogy to the compound 43, with structural data identified as follows.
Compound 44




The pure product as greenish solid was obtained with yield of 65%, and m.p. is 211-212° C.
Elementary Analysis/%:
Calculated value: C, 56.30; H, 4.72; N, 28.72.
Measured value: C, 56.63; H, 4.615; N, 28.9.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.47-4.48 (d, 2H, J=6.0, CH2), 5.41 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.26-7.29 (m, 2H, J=6.0, 6.6, 7.2 Hz, Ar—H), 7.52-7.53 (d, 1H, J=7.2 Hz, Ar—H), 7.60-7.62 (t, 1H, J=6.0, 7.2 Hz, Ar—H), 7.99 (s, 2H, CH), 8.84 (s, 1H, NH).
IR(KBr) υ (cm−1): 3440 (—NH2), 2960 (—CH3), 2920 (—CH2), 1650 (—C═O), 1505 (—Ar).
Compound 45




The pure product as white solid was obtained with yield of 71%, and m.p. is 132-133° C.
Elementary Analysis/%:
Calculated value: C, 60.52; H, 5.68; N, 29.06.
Measured value: C, 60.49; H, 5.805; N, 29.51.
1H NMR (600 MHz, DMSO-d6): δ 2.27 (s, 3H, CH3), 2.32 (s, 3H, CH3), 4.45 (s, 2H, CH2), 5.36 (s, 2H, CH2), 6.90 (s, 2H, NH2), 7.24 (s, 2H, Ar—H), 7.74-7.75 (d, 2H, J=5.4 Hz, Ar—H), 7.96 (s, 2H, CH), 8.93 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.5, 35.4, 47.9, 83.4, 108.5, 127.2, 128.3, 130.5, 133.7, 148.8, 154.5, 160.3, 164.5, 166.6.
Compound 46




The pure product as greenish solid was obtained with yield of 60%, and m.p. is 205-206° C.
Elementary Analysis/%:
Calculated value: C, 56.30; H, 4.72; N, 28.72.
Measured value: C, 56.79; H, 4.942; N, 28.37.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.48-4.49 (d, 2H, J=6.0 Hz, CH2), 5.43 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.28-7.31 (t, 2H, J=7.2 Hz, Ar—H), 7.53-7.56 (m, 1H, J=6.0, 6.0, 7.2 Hz, Ar—H), 7.62-7.64 (m, 1H, J=6.0, 6.6, 7.2, 7.8 Hz, Ar—H), 8.01 (s, 2H, CH), 8.85-8.86 (d, 1H, J=4.8 Hz, NH).
IR(KBr) υ (cm−1): 3440 (—NH2), 2960 (—CH3), 2920 (—CH2), 1650 (—C═O), 1505 (—Ar), 1005 (—C—F).
Compound 47




The pure product as greenish solid was obtained with yield of 93%, and m.p. is 239-240° C.
Elementary Analysis/%:
Calculated value: C, 52.17; H, 4.38; N, 30.42.
Measured value: C, 52.60; H, 4.68; N, 30.35.
1H NMR (600 MHz, DMSO-d6): δ 2.27 (s, 3H, CH3), 4.49-4.50 (d, 2H, J=5.4 Hz, CH2), 5.38 (s, 2H, CH2), 6.88 (s, 2H, NH2), 8.00 (s, 2H, CH), 8.05-8.07 (d, 2H, J=8.4 Hz, Ar—H), 8.28-8.29 (d, 2H, J=9.0 Hz, Ar—H), 9.32 (s, 1H, NH).
MS (EI) m/z (%): 369 (M+, 18).
Compound 48




The pure product as white solid was obtained with yield of 84%, and m.p. is 165-167° C.
Elementary Analysis/%:
Calculated value: C, 53.71; H, 4.51; N, 27.40.
Measured value: C, 53.37; H, 4.778; N, 27.32.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.50-4.51 (d, 2H, J=5.4 Hz, CH2), 5.41 (s, 2H, CH2), 6.92 (s, 2H, NH2), 7.54-7.56 (d, 2H, J=8.4 Hz, Ar—H), 7.88-7.90 (d, 2H, J=8.4 Hz, Ar—H), 8.02 (s, 2H, CH), 9.11 (s, 1H, NH).
MS (EI) m/z (%): 357 (M+, 12).
Compound 49




The pure product as white solid was obtained with yield of 77%, and m.p. is 217-218° C.
Elementary Analysis/%:
Calculated value: C, 53.71; H, 4.51; N, 27.40.
Measured value: C, 53.51; H, 4.851; N, 27.86.
1H NMR (600 MHz, DMSO-d6): δ 2.27 (s, 3H, CH3), 4.43-4.44 (d, 2H, J=5.4 Hz, CH2), 5.41 (s, 2H, CH2), 6.91 (s, 2H, NH2), 7.35-7.36 (d, 1H, J=7.2 Hz, ArH), 7.38-7.39 (d, 1H, J=7.8 Hz, ArH), 7.41-7.42 (d, 1H, J=6.6 Hz, ArH), 7.45-7.46 (d, 1H, J=7.8 Hz, ArH), 7.96 (s, 2H, CH), 8.92 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.8, 34.5, 47.5, 83.1, 107.8, 127.0, 127.9, 130.7, 132.1, 132.8, 147.0, 153.1, 159.8, 163.2, 164.8, 166.7, 168.5.
Compound 50




The pure product as greenish solid was obtained with yield of 99%, and m.p. is 213-214° C.
Elementary Analysis/%:
Calculated value: C, 53.71; H, 4.51; N, 27.40.
Measured value: C, 53.27; H, 4.777; N, 27.51.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.48-4.49 (d, 2H, J=5.4 Hz, CH2), 5.39 (s, 2H, CH2), 6.89 (s, 2H, NH2), 7.48-7.51 (d, 1H, J=8.4, 7.8 Hz, Ar—H), 7.59-7.61 (d, 1H, J=8.4 Hz, Ar—H), 7.81-7.82 (d, 1H, J=7.8 Hz, Ar—H), 7.89 (s, 1H, A-H), 8.00 (s, 2H, CH), 9.13-9.15 (t, 1H, J=5.4, 5.4 Hz, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.1, 36.1, 47.6, 82.4, 108.7, 126.9, 128.7, 139.1, 131.4, 132.9, 133.7, 148.4, 155.2, 161.1, 165.2, 167.2.
Compound 51




The pure product as greenish solid was obtained with yield of 85%, and m.p. is >260° C.
Elementary Analysis/%:
Calculated value: C, 47.71; H, 3.75; N, 27.82.
Measured value: C, 47.61; H, 4.118; N, 27.79.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.48-4.49 (d, 2H, J=4.8 Hz, CH2), 5.43 (s, 2H, CH2), 6.92 (s, 2H, NH2), 7.67-7.68 (d, 1H, J=8.4, Ar—H), 8.01 (s, 2H, CH), 8.21-8.22 (d, 1H, J=7.8 Hz, Ar—H), 8.32 (s, 1H, Ar—H), 9.20 (s, 1H, NH).
IR(KBr) υ (cm−1): 3440 (—NH2), 2960 (—CH3), 2920 (CH2), 1640 (—C═O), 1505 (—Ar), 1355 (—NO2).
Compound 52




The pure product as yellow solid was obtained with yield of 54%, and m.p. is 233-234° C.
Elementary Analysis/%:
Calculated value: C, 47.77; H, 4.01; N, 24.37.
Measured value: C, 47.78; H, 4.502; N, 24.64.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.45-4.46 (d, 2H, J=5.4 Hz, CH2), 5.44 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.35 (s, 1H, Ar—H), 7.37-7.38 (d, 1H, J=8.4 Hz, Ar—H), 7.41-7.42 (d, 1H, J=7.2 Hz, Ar—H), 7.63-7.64 (d, 1H, J=8.4 Hz, Ar—H), 7.99 (s, 2H, CH), 8.94 (s, 1H, NH).
IR(KBr) υ (cm−1): 3440 (—NH2), 2960 (—CH3), 2920 (—CH2), 1640 (—C═O), 1505 (—Ar).
Compound 53




The pure product as white solid was obtained with yield of 63%, and m.p. is 188-189° C.
Elementary Analysis/%:
Calculated value: C, 52.71; H, 4.51; N, 30.42.
Measured value: C, 52.88; H, 4.917; N, 30.54.
1H NMR (600 MHz, DMSO-d6): δ 2.28 (s, 3H, CH3), 4.53 (s, 2H, CH2), 5.39 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.78 (s, 1H, CH), 7.99 (s, 1H, Ar—H), 8.04 (s, 1H, Ar—H), 8.31 (s, 1H, Ar—H), 8.38 (s, 1H, Ar—H), 8.70 (s, 1H, CH), 9.45 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.4, 35.4, 46.6, 83.1, 108.3, 127.1, 127.4, 128.8, 130.4, 133.2, 148.2, 151.7, 154.5, 160.9, 165.7, 167.6.
Additional Embodiment 4




Preparation of Compound 88
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol iodo-4-nitrophenoxypropyne were dissolved in 5 ml anhydrous acetonitrile. 0.05 mmol CuI and 2 mmol triethylamine were added respectively thereto, and stirred for 12 hours at 40-50° C. Upon addition of water at stirring, the solid precipitated out. The solid precipitant was filtered by suction, and dried to give the yellow solid, with yield of 91%, and m.p. is 198-199° C.
Elementary Analysis/%:
Calculated value: C, 42.68; H, 3.36; N, 18.61.
Measured value: C, 42.64; H, 3.43; N, 19.67.
1H NMR (400 MHz, DMSO-d6): δ 2.00 (s, 3H, CH3), 4.03 (s, 2H, CH2), 4.94 (s, 2H, CH2), 6.43 (s, 2H, NH2), 7.58-7.62 (m, 3H, Ar—H), 7.81 (s, 2H, Ar—H), 8.29 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3426 (—NH2), 2960 (—CH3), 2920 (—CH2), 1722 (—C═O), 1505 (—Ar).
The compounds 89-109 are prepared in analogy to the compound 88, with structural data identified as follows.
Compound 89




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 185-187° C.
Elementary Analysis/%:
Calculated value: C, 39.65; H, 2.91; N, 17.34.
Measured value: C, 39.28; H, 3.16; N, 17.79.
1H NMR (400 MHz, DMSO-d6): δ 2.36 (s, 3H, CH3), 4.94 (s, 2H, CH2), 5.50 (s, 2H, CH2), 7.00 (s, 2H, NH2), 7.55-7.59 (m, 2H, Ar—H), 7.76 (d, 2H, J=4.0 Hz, Ar—H), 8.18 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3414 (—NH2), 2962 (—CH3), 2925 (—CH2), 1737 (—C═O), 1500 (—Ar).
Compound 90




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 185-187° C.
Elementary Analysis/%:
Calculated value: C, 39.65; H, 2.91; N, 17.34.
Measured value: C, 39.74; H, 2.86; N, 17.15.
1H NMR (400 MHz, DMSO-d6): δ 2.50 (s, 3H, CH3), 4.94 (s, 2H, CH2), 5.51 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.57 (d, 2H, J=2.4 Hz, Ar—H), 7.70 (d, 2H, J=2.4 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3473 (—NH2), 2962 (—CH3), 2920 (—CH2), 1731 (—C═O), 1500 (—Ar).
Compound 91




The pure product as yellow solid was obtained with yield of 84%, and m.p. is 192-193° C.
Elementary Analysis/%:
Calculated value: C, 39.65; H, 2.91; N, 17.34.
Measured value: C, 39.38; H, 2.73; N, 17.23.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 5.37 (s, 2H, CH2), 5.48 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.59 (d, 2H, J=4.0 Hz, Ar—H), 7.94 (d, 2H, J=4.0 Hz, Ar—H), 8.25 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3409 (—NH2), 3000 (—CH3), 2920 (—CH2), 1720 (—C═O), 1505 (—Ar).
Compound 92




The pure product as yellow solid was obtained with yield of 75%, and m.p. is 200-201° C.
Elementary Analysis/%:
Calculated value: C, 42.51; H, 3.57; N, 17.50.
Measured value: C, 42.44; H, 3.58; N, 17.67.
1H NMR (400 MHz, DMSO-d6): δ 2.08 (s, 3H, CH3), 2.50 (s, 3H, CH3), 5.35 (s, 2H, CH2), 5.51 (s, 2H, CH2), 7.05 (s, 2H, NH2), 7.32 (d, 2H, J=8.0 Hz, Ar—H), 7.83 (d, 2H, J=8.0 Hz, Ar—H), 8.24 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3428 (—NH2), 3005 (—CH3), 2920 (—CH2), 1734 (—C═O), 1505 (—Ar).
Compound 93




The pure product as yellow solid was obtained with yield of 82%, and m.p. is 187-188° C.
Elementary Analysis/%:
Calculated value: C, 41.04; H, 3.01; N, 17.95.
Measured value: C, 41.05; H, 3.30; N, 18.18.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.39 (s, 2H, CH2), 5.60 (s, 2H, CH2), 7.02 (s, 2H, NH2), 7.34 (d, 2H, J=8.0 Hz, Ar—H), 7.69 (s, 1H, Ar—H), 7.87 (d, 1H, J=8.0 Hz, Ar—H), 8.26 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3428 (—NH2), 3015 (—CH3), 2920 (—CH2), 1716 (—C═O), 1500 (—Ar).
Compound 94




The pure product as yellow solid was obtained with yield of 73%, and m.p. is 207-209° C.
Elementary Analysis/%:
Calculated value: C, 41.04; H, 3.01; N, 17.95.
Measured value: C, 40.87; H, 3.42; N, 18.01.
1H NMR (400 MHz, DMSO-d6): δ 2.39 (s, 3H, CH3), 5.42 (s, 2H, CH2), 5.51 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.86 (d, 2H, J=16.0 Hz, Ar—H), 8.10 (s, 2H, Ar—H), 8.28 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3437 (—NH2), 3010 (—CH3), 2920 (—CH2), 1732 (—C═O), 1500 (—Ar), 1005 (—C—F).
Compound 95




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 192-193° C.
Elementary Analysis/%:
Calculated value: C, 38.80; H, 2.85; N, 19.80.
Measured value: C, 38.88; H, 3.01; N, 20.05.
1H NMR (400 MHz, DMSO-d6): δ 2.39 (s, 3H, CH3), 5.36 (s, 2H, CH2), 5.55 (s, 2H, CH2), 6.98 (s, 2H, NH2), 7.36 (s, 2H, Ar—H), 8.01 (s, 2H, Ar—H), 8.24 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3422 (—NH2), 3020 (—CH3), 2920 (—CH2), 1728 (—C═O), 1508 (—Ar), 1355 (—NO2).
Compound 96




The pure product as yellow solid was obtained with yield of 73%, and m.p. is 183-184° C.
Elementary Analysis/%:
Calculated value: C, 41.04; H, 3.01; N, 17.95.
Measured value: C, 40.87; H, 3.42; N, 18.01.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.48 (s, 2H, CH2), 5.47 (s, 2H, CH2), 6.96 (s, 2H, NH2), 8.28 (s, 1H, pyrimidine CH), 8.27-8.31 (m, 2H, Ar—H), 9.10 (s, 1H, Ar—H).
IR(KBr) υ (cm−1): 3457 (—NH2), 3010 (—CH3), 2920 (—CH2), 1647 (—C═O), 1508 (—Ar), 1355 (—NO2).
EI-MS (m/z, %): 529 (M+, 5).
Compound 97




The pure product as yellow solid was obtained with yield of 78%, and m.p. is 201-202° C.
Elementary Analysis/%:
Calculated value: C, 38.80; H, 2.85; N, 19.80.
Measured value: C, 38.82; H, 2.75; N, 20.23.
1H NMR (400 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.45 (s, 2H, CH2), 5.52 (s, 2H, CH2), 7.00 (s, 2H, NH2), 8.28 (s, 1H, pyrimidine CH), 8.07-8.10 (m, 2H, Ar—H), 8.36-8.38 (m, 2H, Ar—H).
IR(KBr) υ (cm−1): 3381 (—NH2), 3010 (—CH3), 2920 (—CH2), 1735 (—C═O), 1508 (—Ar), 1350 (—NO2).
Compound 98




The pure product as yellow solid was obtained with yield of 96%, and m.p. is 200-202° C.
Elementary Analysis/%:
Calculated value: C, 36.32; H, 2.67; N, 15.88.
Measured value: C, 36.57; H, 2.63; N, 15.87.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.36 (s, 2H, CH2), 5.59 (s, 2H, CH2), 7.00 (s, 2H, NH2), 7.49 (s, 2H, Ar—H), 7.73 (s, 2H, Ar—H), 8.26 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3414 (—NH2), 3011 (—CH3), 2923 (—CH2), 1735 (—C═O), 1508 (—Ar).
Compound 99




The pure product as yellow solid was obtained with yield of 59%, and m.p. is 197-198° C.
Elementary Analysis/%:
Calculated value: C, 39.79; H, 3.13; N, 20.27.
Measured value: C, 39.76; H, 3.38; N, 20.61.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 4.49 (s, 2H, CH2), 5.44 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.50 (d, 2H, J=3.2 Hz, Ar—H), 7.83 (d, 2H, J=4.0 Hz, Ar—H), 8.30 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3363 (—NH2), 3010 (—CH3), 2920 (—CH2), 1628 (—C═O), 1501 (—Ar).
Compound 100




The pure product as yellow solid was obtained with yield of 45%, and m.p. is 186-188° C.
Elementary Analysis/%:
Calculated value: C, 39.79; H, 3.13; N, 20.27.
Measured value: C, 40.16; H, 3.39; N, 20.22.
1H NMR (400 MHz, DMSO-d6): δ 2.32 (s, 3H, CH3), 4.49 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.91 (s, 2H, NH2), 7.43-7.48 (m, 4H, Ar—H), 8.10 (s, 2H, Ar—H), 8.28 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3456 (—NH2), 3010 (—CH3), 2920 (—CH2), 1650 (—C═O), 1505 (—Ar).
Compound 101




The pure product as yellow solid was obtained with yield of 86%, and m.p. is 223-224° C.
Elementary Analysis/%:
Calculated value: C, 38.88; H, 3.06; N, 22.67.
Measured value: C, 39.25; H, 2.89; N, 22.96.
1H NMR (400 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.03 (s, 2H, CH2), 4.95 (s, 2H, CH2), 6.43 (s, 2H, NH2), 7.59 (s, 2H, Ar—H), 7.81 (s, 2H, Ar—H), 8.26 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3431 (—NH2), 3010 (—CH3), 2920 (—CH2), 1648 (—C═O), 1505 (—Ar), 1346 (—NO2).
Compound 102




The pure product as yellow solid was obtained with yield of 63%, and m.p. is 218-220° C.
Elementary Analysis/%:
Calculated value: C, 42.78; H, 3.59; N, 21.82.
Measured value: C, 42.93; H, 3.62; N, 21.65.
1H NMR (400 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.49 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.89 (s, 2H, NH2), 7.48-7.52 (m, 5H, Ar—H), 8.28 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3440 (—NH2), 3010 (—CH3), 2920 (—CH2), 1644 (—C═O), 1505 (—Ar).
Compound 103




The pure product as yellow solid was obtained with yield of 84%, and m.p. is 231-233° C.
Elementary Analysis/%:
Calculated value: C, 39.79; H, 3.13; N, 20.27.
Measured value: C, 39.78; H, 2.73; N, 20.74.
1H NMR (400 MHz, DMSO-d6): δ 2.10 (s, 3H, CH3), 4.50 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.90 (s, 2H, NH2), 7.53 (d, 2H, J=8.0 Hz, Ar—H), 7.88 (d, 2H, J=12.0 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3425 (—NH2), 3010 (—CH3), 2920 (—CH2), 1651 (—C═O), 1500 (—Ar).
Compound 104




The pure product as yellow solid was obtained with yield of 56%, and m.p. is 217-219° C.
Elementary Analysis/%:
Calculated value: C, 41.13; H, 3.24; N, 20.98.
Measured value: C, 41.26; H, 3.19; N, 21.04.
1H NMR (400 MHz, DMSO-d6): δ 2.09 (s, 3H, CH3), 4.50 (s, 2H, CH2), 5.47 (s, 2H, CH2), 6.95 (s, 2H, NH2), 7.28 (s, 2H, Ar—H), 7.93 (s, 2H, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.95 (s, 1H, NH).
IR(KBr) υ (cm−1): 3429 (—NH2), 3010 (—CH3), 2920 (—CH2), 1650 (—C═O), 1500 (—Ar).
Compound 105




The pure product as yellow solid was obtained with yield of 55%, and m.p. is 204-206° C.
Elementary Analysis/%:
Calculated value: C, 41.13; H, 3.24; N, 20.98.
Measured value: C, 41.26; H, 3.19; N, 21.04.
1H NMR (400 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.49 (s, 2H, CH2), 5.49 (s, 2H, CH2), 7.04 (s, 2H, NH2), 7.21 (s, 2H, Ar—H), 7.56 (d, 2H, J=4.0 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.75 (s, 1H, NH).
IR(KBr) υ (cm−1): 3347 (—NH2), 3010 (—CH3), 2920 (—CH2), 1650 (—C═O), 1500 (—Ar), 1050 (—C—F).
Compound 106




The pure product as yellow solid was obtained with yield of 48%, and m.p. is 180-182° C.
Elementary Analysis/%:
Calculated value: C, 42.60; H, 3.79; N, 20.46.
Measured value: C, 42.90; H, 3.53; N, 20.32.
1H NMR (400 MHz, DMSO-d6): δ 2.27 (s, 3H, CH3), 2.36 (s, 3H, CH3), 4.45 (s, 2H, CH2), 5.36 (s, 2H, CH2), 6.90 (s, 2H, NH2), 7.24 (s, 2H, Ar—H), 7.74 (d, 2H, J=4.0 Hz, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.93 (s, 1H, NH).
IR(KBr) υ (cm−1): 3407 (—NH2), 3010 (—CH3), 2920 (—CH2), 1686 (—C═O), 1502 (—Ar).
Compound 107




The pure product as yellow solid was obtained with yield of 63%, and m.p. is 183-184° C.
Elementary Analysis/%:
Calculated value: C, 38.88; H, 3.06; N, 22.67.
Measured value: C, 38.40; H, 3.35; N, 22.19.
1H NMR (400 MHz, DMSO-d6): δ 2.39 (s, 3H, CH3), 5.42 (s, 2H, CH2), 5.54 (s, 2H, CH2), 6.92 (s, 2H, NH2), 8.01 (s, 2H, Ar—H), 8.26 (s, 1H, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.86 (s, 1H, NH).
IR(KBr) υ (cm−1): 3436 (—NH2), 3010 (—CH3), 2920 (—CH2), 1745 (—C═O), 1524 (—Ar), 1355 (—NO2).
Compound 108




The pure product as yellow solid was obtained with yield of 79%, and m.p. is 202-204° C.
1H NMR (400 MHz, DMSO-d6): δ 2.28 (s, 3H, CH3), 4.53 (s, 2H, CH2), 5.39 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.78 (s, 2H, Ar—H), 8.01 (s, 2H, Ar—H), 8.38 (s, 1H, pyrimidine CH).
IR(KBr) υ (cm−1): 3405 (—NH2), 3010 (—CH3), 2920 (—CH2), 1654 (—C═O), 1524 (—Ar), 1355 (—NO2).
Compound 109




The pure product as yellow solid was obtained with yield of 90%, and m.p. is 200-202° C.
Elementary Analysis/%:
Calculated value: C, 36.39; H, 2.86; N, 18.56.
Measured value: C, 36.74; H, 2.77; N, 18.36.
1H NMR (400 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.45 (s, 2H, CH2), 5.44 (s, 2H, CH2), 6.94 (s, 2H, NH2), 7.35-7.39 (m, 4H, Ar—H), 8.28 (s, 1H, pyrimidine CH), 8.94 (s, 1H, NH).
IR(KBr) υ (cm−1): 3449 (—NH2), 3010 (—CH3), 2920 (—CH2), 1641 (—C═O), 1524 (—Ar).
Embodiment 5




Preparation of Compound 54
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol benzoylpropyne ester were dissolved in a solvent of 6 ml acetonitrile. 0.01 mmol blue vitriol and 0.1 mmol sodium ascorbate were added thereto respectively. The reaction liquid was stirred over night at 0-20° C., and a significant amount of white or greenish solid precipitated out. It was extracted in ethyl acetate, and the organic phase was dried over anhydrous sodium sulfate over night. The anhydrous sodium sulfate solid was removed, and the organic phase was precipitated and purified by column chromatography on silica gel (G type) with gradient elution (acetone:petroleum ether=1:1), to give the pure product as white solid, with yield of 53%, mp: 135-137° C.
Elementary Analysis/%:
Calculated value: C, 48.24; H, 3.81; N, 19.86.
Measured value: C, 47.75; H, 4.06; N, 19.63.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.97 (s, 2H, CH2), 5.24 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.07 (d, 1H, Ar—H), 7.32-7.34 (t, 1H, Ar—H), 7.59 (d, 1H, Ar—H), 8.00 (s, 1H, 1,2,3-triazole-H), 8.17 (s, 1H, pyrimidine CH).
The compounds 55-67 were prepared in analogy to the compound 54, with its structural data identified as follows:
Compound 55




The pure product as white solid was obtained with yield of 73%, and m.p. is 163-165° C.
Elementary Analysis/%:
Calculated value: C, 53.67; H, 4.75; N, 20.86.
Measured value: C, 53.53; H, 5.00; N, 20.82.
1H NMR (600 MHz, DMSO-d6): δ 2.26 (s, 3H, CH3), 2.31 (s, 3H, CH3), 4.77 (s, 2H, CH2), 5.23 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.94 (s, 2H, NH2), 6.78 (d, 1H, Ar—H), 6.94 (t, 1H, Ar—H), 7.27-7.28 (d, 1H, Ar—H), 8.00 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 56




The pure product as white solid was obtained with yield of 77%, and m.p. is 152-153° C.
Elementary Analysis/%:
Calculated value: C, 52.51; H, 4.41; N, 21.61.
Measured value: C, 52.39; H, 4.57; N, 21.62.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.82 (s, 2H, CH2), 5.23 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.95 (s, 2H, NH2), 6.93 (s, 2H, Ar—H), 7.31-7.32 (d, 2H, Ar—H), 8.00 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 57




The pure product as white solid was obtained with yield of 65%, and m.p. is 149-151° C.
Elementary Analysis/%:
Calculated value: C, 58.69; H, 5.47; N, 22.81.
Measured value: C, 58.42; H, 5.75; N, 22.38.
1H NMR (600 MHz, DMSO-d6): δ 2.22 (s, 3H, CH3), 2.31 (s, 3H, CH3), 4.74 (s, 2H, CH2), 5.22 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 6.78-6.79 (t, 2H, Ar—H), 7.05-7.07 (t, 2H, Ar—H), 8.01 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 58




The pure product as white solid was obtained with yield of 53%, and m.p. is 128-129° C.
Elementary Analysis/%:
Calculated value: C, 56.24; H, 5.24; N, 21.86.
Measured value: C, 56.47.42; H, 5.14; N, 22.05.
1H NMR (600 MHz, DMSO-d6): δ 2.22 (s, 3H, OCH3), 2.31 (s, 3H, CH3), 4.71 (s, 2H, CH2), 5.22 (d, 2H, CH2), 5.44 (s, 2H, CH2), 6.96 (s, 2H, NH2), 6.83 (s, 4H, Ar—H), 8.00 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 59




The pure product as white solid was obtained with yield of 53%, and m.p. is 138-140° C.
Elementary Analysis/%:
Calculated value: C, 53.67; H, 4.75; N, 20.86.
Measured value: C, 53.65; H, 5.10; N, 20.89.
1H NMR (600 MHz, DMSO-d6): δ 2.17 (s, 3H, CH3), 2.31 (s, 3H, CH3), 4.84 (s, 2H, CH2), 5.22 (d, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 6.84 (d, 1H, Ar—H), 7.13-7.15 (t, 1H, Ar—H), 7.22 (s, 1H, Ar—H), 8.00 (s, 1H, triazole CH), 8.16 (s, 1H, pyrimidine CH).
Compound 60




The pure product as white solid was obtained with yield of 70%, and m.p. is 144-146° C.
Elementary Analysis/%:
Calculated value: C, 50.19; H, 3.96; N, 20.66.
Measured value: C, 50.62; H, 3.52; N, 20.80.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.93 (s, 2H, CH2), 5.24 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.05-7.09 (m, 1H, Ar—H), 7.11-7.15 (m, 1H, Ar—H), 7.45 (q, 1H, Ar—H), 8.00 (s, 1H, triazole CH), 8.16 (s, 1H, pyrimidine CH).
Compound 61




The pure product as white solid was obtained with yield of 69%, and m.p. is 107-110° C.
Elementary Analysis/%:
Calculated value: C, 61.45; H, 6.38; N, 20.47.
Measured value: C, 61.60; H, 6.81; N, 20.40.
1H NMR (600 MHz, DMSO-d6): δ 1.24 (s, 9H, 3CH3), 2.31 (s, 3H, CH3), 4.75 (s, 2H, CH2), 5.23 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 6.81-6.82 (d, 2H, Ar—H), 7.27-7.28 (d, 2H, Ar—H), 8.00 (s, 1H, triazole CH), 8.18 (s, 1H, pyrimidine CH).
Compound 62




The pure product as white solid was obtained with yield of 49%, and m.p. is 138-140° C.
Elementary Analysis/%:
Calculated value: C, 51.19; H, 4.06; N, 19.90.
Measured value: C, 51.36; H, 3.66; N, 20.32.
1H NMR (600 MHz, DMSO-d6): δ 2.55 (s, 3H, CH3), 4.68 (s, 2H, CH2), 5.33-5.34 (d, 2H, CH2), 5.63 (s, 2H, CH2), 7.25 (s, 2H, NH2), 7.04-7.07 (t, 2H, Ar—H), 7.25-7.27 (t, 1H, Ar—H), 7.37-7.39 (q, 1H, Ar—H), 7.61 (s, 1H, triazole CH), 8.18 (s, 1H, pyrimidine CH).
Compound 63




The pure product as white solid was obtained with yield of 72%, and m.p. is 168-171° C.
Elementary Analysis/%:
Calculated value: C, 59.67; H, 5.80; N, 21.98.
Measured value: C, 59.89; H, 5.60; N, 22.10.
1H NMR (600 MHz, DMSO-d6): δ 2.09 (s, 3H, CH3), 2.21 (s, 2H, CH3), 2.31 (s, 3H, CH3), 4.77 (s, 2H, CH2), 5.23 (s, 2H, CH2), 5.44 (s, 2H, CH2), 6.96 (s, 2H, NH2), 6.64 (d, 1H, Ar—H), 6.76 (d, 1H, Ar—H), 6.97 (d, 1H, Ar—H), 8.01 (s, 1H, triazole CH), 8.15 (s, 1H, pyrimidine CH).
Compound 64




The pure product as white solid was obtained with yield of 58%, and m.p. is 160-161° C.
Elementary Analysis/%:
Calculated value: C, 44.61; H, 3.30; N, 18.36.
Measured value: C, 44.71; H, 3.39; N, 18.79.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 5.03 (s, 2H, CH2), 5.24 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.45 (s, 1H, Ar—H), 7.83 (s, 1H, Ar—H), 8.01 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 65




The pure product as white solid was obtained with yield of 77%, and m.p. is 158-160° C.
Elementary Analysis/%:
Calculated value: C, 51.13; H, 4.29; N, 24.55.
Measured value: C, 50.62; H, 4.61; N, 24.20.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.04 (s, 2H, CH2), 5.22 (s, 2H, CH2), 5.44 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.13 (t, 1H, Ar—H), 7.23 (d, 1H, Ar—H), 7.57 (t, 1H, Ar—H), 7.85 (d, 1H, Ar—H), 8.00 (s, 1H, triazole CH), 8.15 (s, 1H, pyrimidine CH).
Compound 66




The pure product as white solid was obtained with yield of 56%, and m.p. is 145-148° C.
Elementary Analysis/%:
Calculated value: C, 51.13; H, 4.29; N, 24.55.
Measured value: C, 50.64; H, 4.67; N, 24.29.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.02 (s, 2H, CH2), 5.24 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.14 (d, 2H, Ar—H), 8.19-8.20 (d, 2H, Ar—H), 8.00 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH).
Compound 67




The pure product as white solid was obtained with yield of 56%, and m.p. is 145-148° C.
Elementary Analysis/%:
Calculated value: C, 47.07; H, 3.72; N, 22.60.
Measured value: C, 47.26; H, 3.83; N, 22.19.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 5.16 (s, 2H, CH2), 5.24 (s, 2H, CH2), 5.45 (s, 2H, CH2), 6.96 (s, 2H, NH2), 7.30 (d, 1H, Ar—H), 7.98 (s, 1H, triazole CH), 8.17 (s, 1H, pyrimidine CH), 8.18 (t, 1H, Ar—H), 8.34 (d, 1H, Ar—H).
Embodiment 6




Preparation of Compound 68
1 mmol 2-methyl-4-amino-5-azidomethylpyrimidine and 1 mmol iodo-4-nitrophenoxypropyne were dissolved in 5 ml anhydrous tetrahydrofuran. 0.05 mmol CuBr and 2 mmol triethylamine were added respectively thereto, and stirred for 12 hours at 50-60° C. Upon addition of water at stirring, the solid precipitated out. The solid precipitant was filtered by suction, and dried to give the off-white solid, with yield of 85%, and m.p. is 201-202° C.
Elementary Analysis/%:
Calculated value: C, 48.35; H, 4.06; N, 23.22.
Measured value: C, 48.73; H, 4.38; N, 23.11.
1H NMR (600 MHz, DMSO-d6): δ 2.31 (s, 3H, CH3), 4.34-4.36 (d, 2H, J=8.4 Hz, CH2—NH), 4.64 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.95 (s, 2H, NH2), 6.99 (s, 1H, CH), 7.01 (s, 1H, Ar—H), 7.33-7.35 (dd, 1H, J=3.6 Hz, 9.6 Hz, Ar—H), 7.58-7.59 (d, 1H, J=4.2 Hz, Ar—H), 7.95 (s, 1H, pyrimidine CH), 8.55 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.2, 46.7, 56.7, 58.2, 108.4, 114.7, 124.8, 127.1, 129.6, 130.4, 134.7, 138.5, 142.0, 156.1, 161.2, 165.1, 167.7.
The compounds 69-79 were prepared in analogy to the compound 68, with its structural data identified as follows:
Compound 69




The pure product as black solid was obtained with yield of 78%, and m.p. is 201-202° C.
Elementary Analysis/%:
Calculated value: C, 52.65; H, 4.68; N, 25.28.
Measured value: C, 52.53; H, 4.72; N, 25.39.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.35 (s, 2H, CH2), 4.64 (s, 2H, CH2), 5.46 (s, 2H, CH2), 6.95 (s, 2H, NH2), 6.96-6.97 (m, 2H, Ar—H), 7.33-7.34 (m, 2H, Ar—H), 7.49 (s, 2H), 8.86 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.0, 46.1, 57.2, 60.3, 107.9, 115.6, 124.7, 127.0, 129.6, 130.3, 134.6, 138.4, 143.0, 165.5, 161.4, 164.2, 165.9.
Compound 70




The pure product as off-white solid was obtained with yield of 74%, and m.p. is 117-119° C.
Elementary Analysis/%:
Calculated value: C, 58.84; H, 5.76; N, 26.69.
Measured value: C, 58.93; H, 5.82; N, 26.90.
1H NMR (600 MHz, DMSO-d6): δ 2.22 (s, 3H, CH3), 2.29 (s, 3H, CH3), 4.34-4.35 (d, 2H, J=5.4 Hz, CH2—NH), 4.43 (s, 2H, CH2), 5.39 (s, 2H, CH2), 6.81-6.82 (d, 2H, J=8.4 Hz, Ar—H), 6.94 (s, 2H, NH2), 7.06-7.07 (d, 2H, J=8.4 Hz, Ar—H), 7.91 (s, 1H, CH), 7.98 (s, 1H, CH), 8.61 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 20.2, 24.5, 34.3, 46.9, 66.5, 108.8, 114.8, 116.5, 123.0, 129.8, 145.5, 155.5, 156.4, 161.1, 167.1, 168.8.
Compound 71




The pure product as grey solid was obtained with yield of 61%, and m.p. is 124-125° C.
Elementary Analysis/%:
Calculated value: C, 52.65; H, 4.68; N, 25.28.
Measured value: C, 52.68; H, 5.04; N, 25.78.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.34 (s, 2H, CH2), 4.49 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.95-6.96 (d, 4H, J=7.8 Hz), 7.32-7.33 (d, 2H, J=7.2 Hz, Ar—H), 7.94 (s, 1H), 8.86 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.3, 47.0, 57.4, 67.1, 107.7, 124.3, 126.8, 128.3, 130.6, 138.1, 141.3, 157.9, 160.7, 164.3, 167.3.
Compound 72




The pure product as grey solid was obtained with yield of 91%, and m.p. is 183-184° C.
Elementary Analysis/%:
Calculated value: C, 47.17; H, 3.96; N, 25.89.
Measured value: C, 47.18; H, 3.88; N, 25.54.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.35-4.36 (d, 2H, J=5.4 Hz, CH2), 4.85 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.19-7.20 (d, 1H, 9.6 Hz, Ar—H), 7.96 (s, 1H, CH), 8.19-8.19 (d, 1H, J=2.4 Hz, Ar—H), 8.20 (s, 1H, CH), 8.32-8.33 (d, 1H, J=2.4 Hz, Ar—H), 8.65 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 24.9, 32.9, 45.9, 67.3, 107.8, 113.4, 115.1, 122.3, 124.6, 129.1, 132.8, 144.3, 155.5, 155.9, 160.2, 166.5, 167.9.
Compound 73




The pure product as brown solid was obtained with yield of 71%, and m.p. is 143-144° C.
Elementary Analysis/%:
Calculated value: C, 51.25; H, 4.55; N, 28.13.
Measured value: C, 51.62; H, 4.89; N, 27.89.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.35-4.36 (d, 2H, J=4.8 Hz, CH2), 4.69 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.93 (s, 2H, NH2), 7.13-7.14 (d, 2H, J=9.0 Hz, Ar—H), 7.95 (s, 1H, CH), 8.20-8.22 (d, 2H, J=9.0 Hz, Ar—H), 8.77 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 24.5, 45.8, 57.0, 66.7, 107.3, 124.9, 127.3, 128.8, 130.8, 137.5, 141.5, 155.6, 160.9, 163.5, 166.1.
MS (EI) m/z (%): 398 (M+, 4).
Compound 74




The pure product as brown solid was obtained with yield of 85%, and m.p. is 105-106° C.
Elementary Analysis/%:
Calculated value: C, 47.23; H, 4.20; N, 22.68.
Measured value: C, 47.52; H, 4.58; N, 22.37.
1H NMR (600 MHz, DMSO-d6): δ 2.29 (s, 3H, CH3), 4.34-4.35 (d, 2H, J=5.4 Hz, CH2), 4.49 (s, 2H, CH2), 5.39 (s, 2H, CH2), 6.90-6.91 (d, 4H, J=8.4 Hz), 7.44-7.45 (d, 2H, J=8.4 Hz, Ar—H), 7.93 (s, 1H), 8.67 (s, 1H, NH).
13C NMR (100 MHz, DMSO-d6) δ(ppm): 25.7, 46.6, 58.9, 56.6, 108.4, 124.8, 128.3, 129.0, 131.1, 138.4, 141.6, 156.5, 161.7, 164.5, 167.0.
MS (EI) m/z (%): 432 (M+, 15).
Compound 75




The pure product as brown solid was obtained with yield of 66%, and m.p. is 165-166° C.
Elementary Analysis/%:
Calculated value: C, 56.39; H, 5.52; N, 25.57.
Measured value: C, 56.35; H, 5.67; N, 25.85.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 3.68 (s, 3H, OCH3), 4.34 (s, 2H, CH2), 4.41 (s, 2H, CH2), 5.40 (s, 2H, CH2), 6.85-6.87 (d, 5H, J=12.0 Hz), 6.95 (s, 1H, Ar—H), 8.03 (s, 1H), 8.60 (s, 1H, NH).
IR (KBr) υ (cm−1): 3441 (—NH2), 2950 (—CH3), 2850 (CH2), 1644 (—C═O), 1505 (—Ar).
Compound 76




The pure product as brown solid was obtained with yield of 57%, and m.p. is and m.p. is 177-178° C.
Elementary Analysis/%:
Calculated value: C, 53.80; H, 5.02; N, 24.40.
Measured value: C, 53.77; H, 5.15; N, 24.56.
1H NMR (600 MHz, DMSO-d6): δ 2.26 (s, 3H, CH3), 2.29 (s, 3H, CH3), 4.34 (s, 2H, CH2), 4.47 (s, 2H, CH2), 5.39 (s, 2H, CH2), 6.77-6.79 (d, 1H, J=7.8 Hz, Ar—H), 6.94 (s, 3H), 7.28-7.29 (d, 2H, J=9.0 Hz, Ar—H), 7.93 (s, 1H), 8.64 (s, 1H, NH).
IR(KBr) υ (cm−1): 3446 (—NH2), 2950 (—CH3), 2850 (CH2), 1660 (—C═O), 1505 (—Ar).
Compound 77




The pure product as yellow solid was obtained with yield of 57%, and m.p. is 169-170° C.
Elementary Analysis/%:
Calculated value: C, 59.83; H, 6.08; N, 25.70.
Measured value: C, 60.26; H, 6.02; N, 25.41.
1H NMR (600 MHz, DMSO-d6): δ 2.01 (s, 3H, CH3), 2.20 (s, 3H, CH3), 2.29 (s, 3H, CH3), 4.35-4.36 (s, 2H, J=6.0 Hz, CH2), 4.46 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.63-6.64 (d, 1H, J=8.4 Hz, Ar—H), 6.76-6.77 (d, 1H, J=7.2 Hz), 6.94-6.98 (m, 3H), 7.89 (s, 1H), 8.47 (s, 1H, NH).
IR(KBr) υ (cm−1): 3439 (—NH2), 2950 (—CH3), 2850 (—CH2), 1675 (—C═O), 1505 (—Ar).
Compound 78




The pure product as brown solid was obtained with yield of 71%, and m.p. is 168-169° C.
Elementary Analysis/%:
Calculated value: C, 57.78; H, 5.42; N, 27.75.
Measured value: C, 57.53; H, 5.24; N, 27.64.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.34 (s, 2H, J=6.0 Hz, CH2), 4.49 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.94-6.96 (m, 5H), 7.33-7.34 (m, 2H), 7.94 (s, 1H), 8.67 (s, 1H, NH).
IR(KBr) υ (cm−1): 3447 (—NH2), 2950 (—CH3), 2850 (—CH2), 1665 (—C═O), 1505 (—Ar).
Compound 79




The pure product as brown solid was obtained with yield of 52%, and m.p. is 125-126° C.
Elementary Analysis/%:
Calculated value: C, 50.31; H, 4.22; N, 24.16.
Measured value: C, 50.10; H, 4.63; N, 23.59.
1H NMR (600 MHz, DMSO-d6): δ 2.30 (s, 3H, CH3), 4.35 (s, 2H, J=6.0 Hz, CH2), 4.48 (s, 2H, CH2), 5.41 (s, 2H, CH2), 6.95 (s, 2H), 7.20 (s, 2H), 7.42-7.43 (d, 2H, J=7.2 Hz), 7.93 (s, 1H), 8.52 (s, 1H, NH).
IR(KBr) υ (cm−1): 3427 (—NH2), 2964 (—CH3), 2850 (CH2), 1665 (—C═O), 1505 (—Ar), 1069 (—C—F).







TABLE 1







Synthsized compounds











No.
R1
R2
R3
X










I-1








 











1
H
H
4-NO2
O


2
H
H
4-Cl
O


3
H
H
4-CO2Et
O


4
H
H
3-CF3
O


5
H
2-Cl
5-CH3
O


6
H
2-Cl
4-F
O


7
H
H
4-COOH
O


8
H
2-NO2
4-NO2
O


9
H
H
4-CH2CO2Me
O


10
H
2-Cl
4-Cl
O


11
H
3-CH3
4-Cl
O


12
H
H
H
O


13
H
H
4-OCH3
O


14
H
2-CH3
3-CH3
O


15
H
2-Cl
4-NO2
O


16
H
2-NO2
H
O


17
H
H
4-CH3
O


18
H
2-CH3
H
O


19
H
3-CH3
H
O


20
H
H
4-F
O


21
H
H
4-Br
O


22
H
H
4-CN
O


23
I
H
H
O


24
I
H
4-NO2
O


25
I
3-CH3
4-Cl
O


26
I
H
4-OCH3
O


27
I
2-Cl
4-F
O


28
I
H
4-Cl
O


29
I
2-CH3
4-Cl
O


30
I
2-Cl
4-NO2
O


31
I
H
4-CN
O


80
I
2-NO2
H
O


81
I
H
3-CF3
O


82
I
H
4-F
O


83
I
2-Cl
4-Cl
O


84
I
H
4-Br
O


85
I
2-Br
H
O


86
I
2-Cl
H
O


87
I
H
4-CO2Et
O







I-2








 











32
H
H
4-NO2
O


33
H
H
4-Cl
O


34
H
H
4-F
O


35
H
3-NO2
5-NO2
O


36
H
2-Cl
H
O


37
H
2-F
H
O


38
H
H
4-CH3
O


39
H
3-Cl
H
O


40
H
2-Br
H
O


41
H
3-NO2
H
O


42
H
2-Cl
4-NO2
O


43
H
H
H
NH


44
H
H
4-F
NH


45
H
H
4-CH3
NH


46
H
2-F
H
NH


47
H
H
4-NO2
NH


48
H
H
4-Cl
NH


49
H
2-Cl
H
NH


50
H
3-Cl
H
NH


51
H
2-Cl
4-NO2
NH


52
H
2-Br
H
NH


53
H
3-NO2
H
NH


88
I
H
H
O


89
I
2-Cl
H
O


90
I
3-Cl
H
O


91
I
4-Cl
H
O


92
I
4-CH3
H
O


93
I
2-F
H
O


94
I
4-F
H
O


95
I
4-NO2
H
O


96
I
2-Cl
4-NO2
O


97
I
3-NO2
H
O


98
I
2-Br
H
O


99
I
3-Cl
H
NH


100
I
2-Cl
H
NH


101
I
4-NO2
H
NH


102
I
H
H
NH


103
I
4-Cl
H
NH


104
I
4-F
H
NH


105
I
2-F
H
NH


106
I
4-CH3
H
NH


107
I
2-Cl
4-NO2
NH


108
I
3-NO2
H
NH


109
I
2-Br
H
NH







I-3








 











54
H
2-Cl
4-Cl
O


55
H
3-CH3
4-Cl
O


56
H
H
4-Cl
O


57
H
H
4-CH3
O


58
H
H
4-CH3O
O


59
H
2-CH3
4-Cl
O


60
H
2-Cl
4-F
O


61
H
H
4-C(CH3)3
O


62
H
3-CF3
H
O


63
H
2-CH3
3-CH3
O


64
H
3-Cl
4,5-2Cl
O


65
H
2-NO2
H
O


66
H
H
4-NO2
O


67
H
2-Cl
4-NO2
O


68
H
2-Cl
4-Cl
NH


69
H
2-Cl
H
NH


70
H
H
4-CH3
NH


71
H
H
4-Cl
NH


72
H
2-Cl
4-NO2
NH


73
H
H
4-NO2
NH


74
H
H
4-Br
NH


75
H
H
4-OCH3
NH


76
H
2-CH3
4-Cl
NH


77
H
2-CH3
3-CH3
NH


78
H
H
H
NH


79
H
2-Cl
4-F
NH





In the table, Me—methyl, Et—ethyl.






The compounds of general formula I according to the present invention have superior microbicidal activity against bacterial spot of cucumber, tomato bacterial leaf spot, corynespora leaf spot of cucumber, downy mildew of cucumber, rice sheath blight disease, wheat scab, early blight of tomato, gray mold of cucumber, tobacco brown spot and anthracnose of cucumber, some of which have equivalent or better controlling effects against bacteria or fungi than the commercial microbicides as control.
Embodiment 7
Testing of Microbicidal Activity
Testing Materials: Strain: Cucumber Angular Leaf Spot
Testing method: a cucumber seedling at the stage of 2 seminal leaves was inoculated by spraying a suspension of Cucumber Angular Leaf Spot. Testing agents and control agents were sprayed uniformly on the seminal leaves at A.M. of a sunshine day respectively. After 2 hours, the leaves were inoculated with pathogenic microbes and cultured at humidity. After full occurrence of disease in control group, state of illness was examined according to grading standards, and the controlling effects were measured. Testing results were given in table 2.







TABLE 2







Testing results from some compounds 


(testing concentration of 500 μg/g)











Cucumber 



Compounds
bacterial spot (%)














32
60



33
60



34
60



35
45



36
60



37
70



38
60



39
60



Zhongshengmycin
70.69



Bismerthiazol
73.14



Thiodiazole-copper
65.89










Embodiment 8
Testing of Microbicidal Activity
Testing Materials: Strains: Tomato Bacterial Leaf Spot
(Pseudomonassyringaepv. tomato (Okabe) Young, Dye & Wilkie), Cucumber Angular Leaf Spot, Rice Sheath Blight Disease (Rhizoctonia solani), Corynespora Leaf Spot of Cucumber (Corynespora cassiicola), Downy Mildew of Cucumber (Cucumber Downy Mildew).
Testing Method:
A cucumber seedling at the stage of 2 seminal leaves and a tomato seedling at the stage of 5 leaves were inoclulated by spraying a suspension of spore for Downy Mildew of Cucumber and Corynespora Leaf Spot of Cucumber, and spraying a bacterial suspension for Cucumber Angular Leaf Spot and Tomato Bacterial Leaf Spot. Testing agents and control agents were sprayed uniformly at A.M. of a sunshine day on the cucumber seminal leaves. After 2 hours, the leaves were inoculated with pathogenic microbes and cultured at humidity. Inoculation of the suspension of spore by root irrigation is used for fusarium wilt of cucumber. Strip inoculation is used for Rice Sheath Blight Disease and phytophthora blight of pepper, in which the liquor was sprayed and after 2 hours, inoculation of strips was performed followed by culturing at humidity. After full occurrence of diseases in the control group, state of illness was examined according to grading standards, and a disease index and inhibition rate were calculated. Testing results were given in table 3.







TABLE 3







Testing results from some compounds (testing concentration of 500 μg/g)













Tomato
Cucumber
Rice Sheath
Corynespora
Downy



Bacterial
Angular Leaf
Blight
Leaf Spot of
Mildew of



Leaf Spot
Spot
Disease
Cucumber
Cucumber



Average
Average
Average
Average
Average



inhibition 
inhibition
inhibition
inhibition rate
inhibition


Compounds
rate (%)
rate (%)
rate (%)
(%)
rate (%)















1
N
60
90
50
75


2
N
60
80
55
75


3
N
60
80
80
60


4
N
60
60
70
35


5
N
50
80
70
70


6
N
65
70
90
30


7
N
55
70
70
40


8
N
60
70
70
60


9
N
60
90
60
80


10
N
60
70
70
50


11
N
60
70
65
50


12
N
60
80
70
70


13
N
60
70
80
50


14
N
60
70
90
35


15
N
60
60
80
30


16
N
70
70
80
50


32
70
N
60
N
N


33
70
N
55
N
N


34
60
N
40
N
N


35
80
80
50
N
N


36
80
60
45
N
N


37
70
37
50
N
N


38
95
40
50
N
N


39
80
10
60
N
N


Thiophanate-
N

85
80
20


methyl







Zhongshengmycin
60
70
50
50
40


jingangmycin
N

70
70
70


Dimethomorph
N

90
70
90


Bismerthiazol

73





Thiodiazole-copper

66





Note:


N represents undetermined activity.






Embodiment 9
Testing of Microbicidal Activity
In this method, microbicidal activity was determined from spawn growth rate. Six common microbes associated with commercial crops, vegetable crops and fruit crops were selected as targets for testing: Rice Sheath Blight Disease, gray mold of cucumber, wheat scab, early blight of tomato, Tobacco brown spot and anthracnose of cucumber. The compounds were dissolved in a small amount of acetone, emulsified with Tween-80, and added with distilled water to achieve given concentrations for use. 200 grams (g) potato, 15 g glucose, 15 g agar and 1000 g water were formulated into a culture medium, and sterilized with dishes having a diameter of 9 cm at high temperature and at reduced pressure for 25 minutes. Then 13.5 mL hot medium and 1.5 mL prepared compound solution were mixed uniformly and distributed equally into two dishes. The dishes were placed levelly, and after cooling, 5 mm agar with microbes collected from the culture of strains was inoculated into each dish by a sterile collector. Surface of spawn faces downward and each dish was placed with 2-3 strains. Two blank control groups were established. The dishes were then placed into a sterile, constant temperature drying oven for 48 hours. Diameters of the bacterial plaque were measured. Based on plaque diameter of the control, the efficacy of compounds is indicated by the diameters:

Inhibition %=(control−treatment)/control×100%.

Testing materials: strains: wheat scab (Gibberella zeae), gray mold of cucumber (Botrytis cinerea), early blight of tomato (Alternaria solania), tobacco brown spot (Alternaria alternate (Fries) Keissler), anthracnose of cucumber (GloeosporiumorbiculareArs). Testing results were given in table 4-8.







TABLE 4







Testing results from some compounds 


(testing concentration of 100 μg/g)













Gray mold of

Tobacco brown




cucumber

spot




Average 

Average 



Compound
inhibition
Compound
inhibition



No.
rate (%)
No.
rate (%)
















2
73
2
52



3
70
4
66



4
77
5
56



5
67
7
57



6
63
10
58



7
67
14
60



8
54
32
87



9
79
35
75



10
56
39
50



11
67





12
53





13
49





14
54





15
44





16
82





32
59





33
61





34
63





35
49





36
61





37
98





38
61





39
84





Difenoconazole
73



















TABLE 5







Testing results from some compounds 


(testing concentration of 100 μg/g)













Anthracnose 



Early 



of

Wheat 

blight 



cucumber

scab

of tomato



Average 

Average 

Average


Compound
inhibition
Compound 
inhibition
Compound
inhibition 


No.
rate (%)
No.
rate (%)
No.
rate (%)















2
50
2
51
4
51


4
65
11
54
32
63


10
64
16
63




15
57
33
55




32
67
34
51




35
50
37
51




38
54
38
56




39
52
















TABLE 6







Testing results from some compounds 


(testing concentration of 100 μg/g)













Sheath 
Gray 
Early 



Wheat 
and culm
mold of
blight of



scab
blight of rice
cucumber
tomato



Average 
Average 
Average 
Average 


Compound
inhibition
inhibition 
inhibition 
inhibition


No.
rate (%)
rate (%)
rate (%)
rate (%)














23
59
96
88
57


24
68
97
84
66


25
62
97
96
71


26
55
99
92
69


27
39
94
99
38


28
67
100
100
69


29
67
98
100
60


31
58
97
95
70


82
48
96
96
70


84
62
98
93
58


86
62
99
100
60


Difenoconazole
98
100
96
100
















TABLE 7







Testing results from some compounds















Sheath 







and
Gray 
Early 





culm 
mold 
blight 


Compound 
Concentration
Wheat 
blight 
of
of


No.
(μg/g)
scab
of rice
cucumber
tomato















28
25
43
91
42
0



50
64
98
86
28



100
69
99
90
68


29
25
26
64
79
0



50
45
70
90
25



100
52
96
100
41


Difenoconazole
100
95
91
86
100
















TABLE 8







Testing results from some compounds














Sheath and
Gray mold




Concentration
culm blight
of



No.
(μg/g)
of rice
cucumber
















25
50
61
67




25
25
50




12.5
0
40



26
50
94
88




25
72
55




12.5
58
43



27
50
72
92




25
37
66




12.5
0
59



28
50
98
98




25
96
90




12.5
87
85



29
50
69
91




25
68
72




12.5
33
47



31
50
96
91




25
88
87




12.5
78
75



82
50
100
99




25
90
97




12.5
63
56



84
50
97
94




25
95
90




12.5
73
66



86
50
98
97




25
74
76




12.5
37
40



Difenoconazole
100
100
96










While there has been shown several and alternate embodiments of the present invention, it is to be understood that certain changes can be made as would be known to one skilled in the art without departing from the underlying scope of the present invention as is discussed and set forth above and below including claims. Furthermore, the embodiments described above and claims set forth below are only intended to illustrate the principles of the present invention and are not intended to limit the scope of the present invention to the disclosed elements.

## Claims

1. A 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound, having a structure of formula I:



wherein R1 represents hydrogen or iodine, X represents 0 or NH, Y represents phenyl or substituted phenyl, benzoyl or substituted benzoyl, phenoxyacetyl or substituted phenoxyacetyl; and
wherein each substituent on the benzene ring of Y is H, halogen, nitro, cyano, CF3, C1-4 alkyl, methoxy, C1-2 carboxy or alkoxycarbonyl, the substituents are mono- or multi-substituted at any position of the benzene ring, and the substituents are the same or different.

2. A method for preparing the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound of claim 1,
wherein the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound has a structure of formula I-1;
wherein the structure of formula I-1 is prepared by reacting a compound of formula II with a compound of formula III:




and
wherein in formula I-1 and formula III, R1 is defined as in formula I, R2 is H, halogen, nitro or C1-4 alkyl, and R3 is H, halogen, nitro, cyano, CF3, C1-4 alkyl, methoxy, C1-2 carboxy or carboxylate alkoxycarbonyl.

3. A method for preparing the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound of claim 1,
wherein the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound has a structure of formula I-2;
wherein the structure of formula I-2 is prepared by reacting a compound of formula II with a compound of formula IV:




and
wherein in formula I-2 and formula IV, X is defined as in formula I, R1 represents hydrogen or iodine, R2 is H, halogen or nitro, and R3 is H, halogen, nitro or C1-4 alkyl.

4. A method for preparing the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound of claim 1,
wherein the 2-methyl-4-amino-5-(substituted-1H-1,2,3-triazolyl)methylpyrimidine compound has a structure of formula I-3;
wherein the structure of formula I-3 is prepared by reacting a compound of formula II with a compound of formula V:




and
wherein in formula I-3 and formula V, X is defined as in formula I, R1 represents hydrogen, R2 is H, halogen, nitro, CF3 or C1-4 alkyl, and R3 is H, halogen, nitro, C1-4 alkyl or methoxy.