# Patent

## Title
Three-dimensional structure of complement receptor type 2 and uses thereof

## Abstract

Disclosed is a crystalline human CR2 protein in complex with C3d, and the three dimensional structure of the crystalline complex. Also disclosed are methods of use of the structure, particularly for structure-based identification of compounds that bind to CR2 and inhibit or enhance the binding of CR2 to a natural ligand, that bind to CR2 and agonize or antagonize the receptor, that bind to CR2 and inhibit or enhance CR2 dimerization, or that use the C3-binding ability of CR2 as a drug delivery vehicle. Also disclosed are therapeutic compounds obtained by such methods and uses for such compounds.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. patent application Ser. No. 11/786,788, filed Apr. 12, 2007, now U.S. Pat. No. 8,465,737, which is a continuation of U.S. patent application Ser. No. 10/921,415, filed Aug. 16, 2004, now abandoned, which is a divisional of U.S. patent application Ser. No. 09/834,309, filed Apr. 11, 2001, now U.S. Pat. No. 6,820,011. The entire disclosure of each of the above-identified applications is incorporated herein by reference.


GOVERNMENT SUPPORT
This invention was made with government support under Grant No. NIH R0-1 CA53615 awarded by the National Institutes of Health. The government has certain rights in the invention.


FIELD OF THE INVENTION
This invention relates to the crystallization and resolution of the three-dimensional structure of the human complement receptor type 2 (CR2) protein, and to methods of using such structure, particularly for structure-based drug design of regulatory compounds.
BACKGROUND OF THE INVENTION
Complement receptor type 2 (CR2 or CD21) is a key interface between innate and adaptive immunity by serving as the receptor for complement component C3d, as well as for C3 and fragments of C3 that contain the C3d domain or a portion thereof, including but not limited to C3dg, iC3b and C3b (D. T. Fearon and R. H. Carter, Annu Rev Immun l 13, 127-49 (1995); D. T. Fearon, Semin Immunol 10, 355-61 (1998)). C3d and other CR2-binding C3 fragments that contain C3d or a portion thereof are covalently attached to foreign antigens (such as invading microorganisms) through the action of the classical, alternative or lectin complement pathways (S. K. A. Law and K. B. M. Reid, Complement, D. Male, Ed., In Focus (Oxford, UK:IRL Press., ed. second edition, 1995)). C3d- or other CR2-binding C3 fragment-bound antigens then greatly amplify B cell responses by binding to CR2 through these C3 fragments at the same time as engaging the B cell receptor (BCR) via the bound antigen (R. H. Carter and D. T. Fearon, Science 256, 105-7 (1992); J. C. Cambier, Biochem Soc Trans 25, 441-5 (1997)). The cross-linking of CR2 to the BCR by C3d, C3, or other CR2-binding fragments of C3 that contain C3d or a portion thereof greatly amplifies a signal transduction cascade through the CR2/CD19/CD81 co-activation complex (D. T. Fearon, 1995 ibid.; D. T. Fearon, 1998, ibid.; J. C. Cambier, 1997, ibid; A. K. Matsumoto, et al., J Exp Med 173, 55-64 (199)).
Human CR2 is also the obligate receptor for the Epstein-Barr virus (EBV) through its interactions with the gp350/220 viral membrane protein (J. D. Fingeroth, et al., Proc Natl Acad Sci USA 81, 4510-4 (198)). EBV causes infectious mononucleosis, and is associated with Burkitt's Lymphoma and several other lymphomas and non-lymphoid tumors (M. Okano, Acta Paediatr 87, 11-8 (1998)). In addition, human CR2 serves as a receptor for CD23 (1. P. Aubry et al., Nature 358, 505-7 (1992)) and is thus a receptor for at least three biologically important ligands. Using genetically manipulated mice and animal models, CR2 has been shown to be essential for the development of normal humoral immunity to T-dependent antigens (T. Hebell et al., Science 254, 102-5 (1991); J. M. Ahearn, et al., Immunity 4, 251-62 (1996); H. Molina, et al., Proc Natl Acad Sci USA 93, 3357-61 (1996) as well as possibly play an important role in the maintenance of B cell self-tolerance and the development of autoimmunity (A. P. Prodeus, et al., Immunity 9, 721-31 (1998)). CR2 has also been shown to mediate the interaction of C3-bound HIV-1 as an immune complex with B cells in a fashion that promotes transfer of virus and infection of CD4 T cells (S. Moir, et al., J Exp Med 192, 637-46 (2000). CR2 also mediates direct infection of CR2-expressing T cells or other CR2-expressing cell lineages that are bound by HIV-1 immune complexes containing C3, C3d or other CR2-binding C3 fragments (including, but not limited to, HIV-1 complexed with C3d).
Interactions with all three human CR2 ligands require the first two of 15 or 16 short consensus repeat (SCR) domains (C. A. Lowell, et al., J Exp Med 170, 1931-46 (1989); J. C. Carel et al., J Biol Chem 265, 12293-9 (1990)). SCR domains, like Ig domains, are found in many proteins from both complement and non-complement families, and mediate diverse biological functions (A. P. Wiles, et al., J Mol Bio1272, 253-65 (1997)). Several of the SCR proteins also serve as receptors for important human pathogens. For example, in addition to CR2, CD46 is a Measles Virus receptor (R. E. Dorig et al., Cell 75, 295-305 (1993)), and CD55 is an echovirus receptor (T. Ward, et al., EMBO J 13, 5070-4 (1994); J. M. Bergelson, et al., Proc Natl Acad Sci USA 91, 6245-9 (1994)). Previously determined structures of SCR proteins containing two or four SCR domains have revealed a conserved core structure but variable orientations between domains mediated in part by relatively short 3-8 amino acid inter-SCR linker peptides (A. P. Wiles, et al., 1997, ibid.; P. N. Barlow, et al., J Mol Biol 232, 268-84 (1993); J. M. Casasnovas et al., EMBO J 18, 2911-22 (1999); R. Schwarzenbacher, et al., EMBO J 18, 6228-39 (1999)). As one of the major functions of SCR domains is to mediate protein-protein (such as receptor-ligand) interactions, and at least two SCRs have been found to be required for these interactions, the relative angle and orientation unique to each SCR-containing protein is likely to contribute to both biologic diversity as well as specificity. However, the lack of a high-resolution structure of a receptor-ligand complex in this family has hindered the understanding of the molecular recognition mechanisms of this class of proteins. With regard to the structure of CR2 and the molecular interactions with its ligands, C3d and EBVgp350/220, variable results have been obtained using mutagenesis, monoclonal antibody, and peptide strategies (C. A. Lowell, et al., J Exp Med 170, 1931-46 (1989); D. R. Martin et al., J Exp Med 174, 1299-311 (1991); H. Molina, et al., J Biol Chem 266, 12173-9 (1991); H. Molina et al., J Immunol 153, 789-95 (1994); D. R. Martin et al., J Virol 68, 4716-26 (1994); H. Molina, et al., J Immunol 154, 5426-35 (1995)).
Therefore, there is a need in the art for a three dimensional structure of CR2 in order to better understand the molecular recognition mechanisms of the protein and to enable the identification and/or design of compounds that mimic, enhance, disrupt or compete with the interactions of CR2 with its ligands.
SUMMARY OF THE INVENTION
One embodiment of the present invention relates to a method of structure-based identification of compounds which potentially bind to complement receptor type 2 (CR2) proteins or to a complex of CR2 and its ligand. This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region; and, (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a). The three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region is selected from: (i) a structure defined by atomic coordinates of a three dimensional structure of a crystalline CR2 SCR1-2 region in complex with C3d; (ii) a structure defined by atomic coordinates selected from: (1) atomic coordinates represented in a table selected from the group consisting of Table 2 (CR2-C3d) and Table 3 (CR2 only); and, (2) atomic coordinates that define a three dimensional structure, wherein at least 50% of the structure has an average root-mean-square deviation (RMSD) from backbone atoms in secondary structure elements in at least one domain of a three dimensional structure represented by the atomic coordinates of (1) of equal to or less than about 1.0 Å; and (ii) a structure defined by atomic coordinates derived from CR2 protein molecules arranged in a crystalline manner in a space group R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å.
In one aspect of this embodiment, the step of identifying comprises selecting candidate compounds that potentially bind to and activate CR2.
In another aspect of this embodiment, the method further includes the step of: (c) selecting candidate compounds of (b) that inhibit the binding of CR2 to its ligand. The step (c) of selecting can include: (i) contacting the candidate compound identified in step (b) with CR2 or a fragment thereof and a CR2 ligand or a fragment thereof under conditions in which a CR2-CR2 ligand complex can form in the absence of the candidate compound; and (ii) measuring the binding affinity of the CR2 or fragment thereof to the CR2 ligand or fragment thereof; wherein a candidate inhibitor compound is selected as a compound that inhibits the binding of CR2 to its ligand when there is a decrease in the binding affinity of the CR2 or fragment thereof for the CR2 ligand or fragment thereof, as compared to in the absence of the candidate inhibitor compound. The CR2 ligand can include, but is not limited to, C3d, C3, a CR2-binding fragment of C3 containing C3d, CD23, and Epstein Barr Virus (EBV) gp350/220, or CR2-binding fragments of any of the ligands. In one aspect, the CR2 protein or fragment thereof comprises an amino acid sequence selected from the group of SEQ ID NO:4 and SEQ ID NO:6.
In another aspect of this embodiment, the method further includes the step of: (c) selecting candidate compounds that stabilizes a complex of CR2 with its ligand. Step (c) can include: (i) contacting the candidate compound identified in step (b) with a CR2-CR2 ligand complex, wherein the CR2-CR2 ligand complex comprises CR2 or a fragment thereof and a CR2 ligand, or a fragment thereof; and (ii) measuring the stability of the CR2-CR2 ligand complex of (i), wherein a candidate stabilizer compound is selected as a compound that stabilizes the CR2-CR2 ligand complex when there is an increase in the stability of the complex as compared to in the absence of the candidate stabilizer compound. In this aspect, the ligand is preferably selected from C3d, C3, a CR2-binding fragment of C3 containing C3d, CD23, and CR2-binding fragments of any of the ligands. In this aspect, the CR2 protein or fragment thereof can comprise an amino acid sequence selected from the group of SEQ ID NO:4 and SEQ ID NO:6.
In the method of identifying a compound, the step (a) of identifying can include identifying candidate compounds for binding to the SCR2 domain of the CR2. In one aspect, the step of identifying includes identifying candidate compounds for binding to the interface between the SCR1 and SCR2 domains of CR2. In another aspect, the step of identifying includes identifying candidate compounds for binding to the dimer interface between two CR2 proteins. In yet another aspect, the step of identifying includes identifying candidate compounds for binding to the interface between CR2 and C3d, C3, a CR2-binding fragment of C3 containing C3d, or a fragment thereof. In one aspect, the step of identifying includes identifying candidate compounds for binding to the B strand and the B-C loop of CR2 SCR2 comprising the segment: G79-G80-Y81-K82-183-R84-G85-S86-T87-P88-Y89. In another aspect, the step of identifying includes identifying candidate compounds for binding to a site on the B strand of CR2 SCR2 comprising position K100. In another aspect, the step of identifying includes identifying candidate compounds for binding to a segment of CR2 SCR2 comprising V130-F131-P132-L133. In yet another aspect, the step of identifying comprises identifying candidate compounds for binding to a segment of CR2 SCR2 comprising the fragment T101-N102-F103. In one aspect of the method of identifying, the step of identifying includes identifying candidate compounds for binding to amino acid residues at positions 84 and 86 of an amino acid sequence selected from the group consisting of SEQ ID NO:4 and SEQ ID NO:6.
When the ligand is C3d, C3, or a CR2-binding fragment of C3 containing C3d, the step of identifying can include identifying candidate compounds for binding to, the loop between helix 2-3 of C3d comprising the segment Q68-P69-S70-S71. In another aspect, the step of identifying can include identifying candidate compounds for binding to Helix 5 of C3d comprising the segment S104-Q105-V106-L107-C108-G109-A110-V111-K112-W113-L114-I115-L116-E117-K118-Q119-K120-P121-D122. In another aspect, the step of identifying can include identifying candidate compounds for binding to Helix 7 of C3d comprising the segment N170-S171-L172-P173-G174-S175-I176-T177-K178-A179-G180-D181-F182-L183-E18-4-A185.
The step of identifying a compound in the method of the present invention can include any suitable method of drug design, drug screening or identification, including, but not limited to: directed drug design, random drug design, grid-based drug design, and/or computational screening of one or more databases of chemical compounds.
Yet another embodiment of the present invention relates to a method to identify a compound that inhibits the complement receptor type 2 (CR2)-dependent infection of a host cell by Epstein Barr Virus (EBV). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a cell that expresses CR2 or a ligand binding fragment thereof and an Epstein Barr Virus (EBV) particle under conditions in which the EBV particle can bind to CR2 and infect the cell in the absence of the candidate compound; and (d) measuring the intracellular EBV titer of the cell; wherein a candidate inhibitor compound is selected as a compound that inhibits the EBV titer in the cell, as compared to in the absence of the candidate inhibitor compound.
Yet another embodiment of the present invention relates to a method to identify a compound that inhibits the binding of CD23 to complement receptor type 2 (CR2). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a first cell expressing CR2 or a ligand binding fragment thereof and a second cell expressing a CD23 protein or fragment thereof under conditions in which the CD23 protein or fragment thereof and the CR2 or the ligand binding fragment thereof can bind in the absence of the candidate compound; and (d) measuring a biological activity induced by the interaction of CD23 and CR2 in the first or second cell; wherein a candidate inhibitor compound is selected as a compound that inhibits the biological activity as compared to in the absence of the candidate inhibitor compound. In a preferred embodiment, the biological activity is IgE isotype switching in the first cell.
Yet another embodiment of the present invention relates to a method to identify a compound that inhibits the binding of C3d, C3 or another CR2-binding fragment of C3 containing C3d or a portion thereof, to complement receptor type 2 (CR2). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a cell expressing CR2 or a fragment thereof and C3d, C3, a CR2-binding fragment of C3 containing C3d, or a fragment thereof, under conditions in which the C3d, the C3, the CR2-binding fragment of C3 containing C3d, or a fragment thereof, can bind to CR2 or the fragment thereof and enhance cell activation in the absence of the candidate compound; and (d) measuring the activation of the cell; wherein a candidate inhibitor compound is selected as a compound that inhibits cell activation, as compared to in the absence of the candidate inhibitor compound. In this embodiment, the cell in (c) can include, but is not limited to: a B cell, a T cell, a thymocyte, an epithelial cell, and a mast cell. Activation can be measured by any suitable method including, but not limited to: measurement of cytokine production by the cell, measurement of calcium mobilization in the cell, measurement of lyn tyrosine kinase activity in the cell, measurement of phosphatidylinositol 3′ kinase activity in the cell, measurement of activation of NF-κB, measurement of activation of MAP kinases, measurement of phosphorylation of CD 19 in the cell, and measurement of activation of protein kinase C (PKC) in the cell.
Another embodiment of the present invention relates to a method to inhibit complement receptor type 2 (CR2)-dependent human immunodeficiency virus-1 (HIV-1) infection of cells in a patient. This method includes the steps of administering to a patient infected with HIV-1 an inhibitor compound that inhibits the binding of C3d, C3 or another CR2-binding fragment of C3 containing C3d or a portion thereof, -opsonized HIV-1 to B cells, follicular dendritic cells, T cells or macrophages in the patient. The inhibitor compound is selected by the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a B cell or follicular dendritic cell expressing CR2 or a fragment thereof and C3d, C3, a CR2-binding fragment of C3 containing C3d, or a fragment thereof, under conditions in which the C3d, the C3, the CR2-binding fragment of C3 containing C3d, or the fragment thereof, can bind to CR2 and enhance B cell activation or follicular dendritic cell activation in the absence of the candidate compound; and (d) measuring the activation of the B cell or the follicular dendritic cell, wherein a candidate inhibitor compound is selected as a compound that inhibits B cell activation or follicular dendritic cell activation, as compared to in the absence of the candidate inhibitor compound.
Yet another embodiment of the present invention relates to a method to prepare a vaccine. This method includes linking a compound that increases B cell activation to an antigen to form the vaccine, wherein the compound is selected by the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a B cell expressing CR2 or a fragment thereof and with C3d, C3, a CR2-binding fragment of C3 containing C3d, or a fragment thereof, under conditions in which the C3d, the C3, the CR2-binding fragment of C3 containing C3d, or the fragment thereof, can bind to and activate CR2 in the absence of the candidate compound; and (d) measuring the activation of the B cell; wherein a candidate compound for use in a vaccine is selected as a compound that increases B cell activation, as compared to in the absence of the candidate compound.
Yet another embodiment of the present invention relates to a drug delivery system, which includes: (a) a drug; and, (b) a portion of a CR2 protein selected from the group of: (i) positions on strand B and the B-C loop of SCR2 including: G79-G80-Y81-K82-183-R84-G85-S86-T87-P88-Y89; (ii) position K100 on the B strand of CR2; and, (iii) positions: V130-F131-P132-L133; and (iv) combinations of (i)-(iii). The drug is linked to the portion of CR2.
Yet another embodiment of the present invention relates to an antibody that selectively binds to CR2. The antibody binds to a portion of CR2 selected from the group of: (a) the interface between the SCR1 and SCR2 domains of CR2; (b) the dimer interface between two CR2 proteins; and, (c) the interface between CR2 and C3d. Preferably, an antibody that binds to an interface between CR2 and C3d selectively binds to a site selected from: (i) the B strand and the B-C loop of CR2 SCR2 comprising the segment: G79-G80-Y81-K82-183-R84-G85-S86-T87-P88-Y89; (ii) the B strand of CR2 SCR2 comprising position K100; (iii) a segment of CR2 SCR2 comprising V130-F131-P132-L133; and, (iv) a segment of CR2 SCR2 comprising T101-N102-F103.
Yet another embodiment of the present invention relates to a crystal comprising complement receptor type 2 (CR2) in complex with C3d. The CR2 consists of SEQ ID NO:4, and the C3d consists of SEQ ID NO:7. The crystal effectively diffracts X-rays for the determination of the atomic coordinates of the CR2 in complex with C3d to a resolution of greater than 2.0 Å, and the crystal has a space group R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å.
Another embodiment of the present invention is a therapeutic composition that, when administered to an animal, enhances B cell responses in the animal. The therapeutic composition comprises a compound that stimulates the activity of a complement receptor type 2 (CR2). The compound is identified by the method that includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) synthesizing the candidate compound; and (d) selecting candidate compounds that bind to and activate CR2.
Yet another embodiment relates to a therapeutic composition that, when administered to an animal, inhibits the biological activity of complement receptor type 2 (CR2) in the animal. The therapeutic composition includes a compound that inhibits the activity of a complement receptor type 2 (CR2). The compound is identified by the method that includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) synthesizing the candidate compound; and (d) selecting candidate compounds that inhibit the biological activity of CR2. Preferably, the compounds inhibit the formation of a complex between CR2 and a CR2 ligand. The ligand can include, C3d, C3, CR2-binding fragments of C3 containing C3d, CD23 and Epstein Barr Virus (EBV), and CR2-binding fragments any of the ligands. In one aspect, the compound inhibits the activation of CR2.
Yet another embodiment of the present invention relates to a method of preparing complement receptor type 2 (CR2) proteins having modified biological activity. This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described in detail above; (b) analyzing the three dimensional structure to the three-dimensional structure of the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify at least one site in the structure contributing to the biological activity of CR2; and (c) modifying the at least one site in a CR2 protein to alter the biological activity of the CR2 protein.
Yet another embodiment of the present invention relates to an isolated protein comprising a mutant C3d. The protein comprises an amino acid sequence that differs from SEQ ID NO:7 by an amino acid substitution selected from the group of: a non-asparagine amino acid residue at position 170, a non-isoleucine amino acid residue at position 115, and/or a non-leucine amino acid residue at position 116. The C3d mutant protein has reduced binding to complement receptor type 2 (CR2), as compared to a wild-type C3d protein. In one aspect, the mutant protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8 and SEQ ID NO:9.



BRIEF DESCRIPTION OF THE DRAWINGS OF THE INVENTION
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
FIG. 1 A shows an overall view of the structure of CR2 binding to C3d, showing only SCR2 contacting one portion of the edge of C3d.
FIG. 1 B shows the overall structure showing a second CR2-C3d complex (colored in light blue and grey) that dimerizes with the first one in FIG. 1A.
FIG. 2 shows the structure of CR2: A is a ribbon representation of the CR2 SCR1 (in red) and SCR2 (in yellow) structures, showing the SCR fold and the packing of the two domains to form a V shape;
B is a representation of the structure and packing interaction at the interface of CR2 SCR1 and SCR2 domains;
C is a surface representation of the two-domain arrangement of CR2;
D is a representation of the dimerization of CR2 through interactions between SCR1 of each molecule; and
E is a sequence alignment between human CR2 (hCR2) SCR1-2 domains (SEQ ID NO:4) and mouse CR2 (mCR2) SCR1-2 domains (SEQ ID NO:6).
FIG. 3 shows the structure at the CR2-C3d interface: A and B are representations of the surface features of the interface area on C3d (in cyan) and CR2 molecule (in yellow);
C shows the structure of the CR2 SCR2-C3d complex;
D and E show the detailed interactions between CR2 (in yellow) and C3d (in cyan) in two angles; and
F shows the human C3d sequence (SEQ ID NO:7) with secondary structure assigned on top of the corresponding sequences.
FIG. 4A is a digitized image of a native gel shift assay of the binding between CR2 and C3d wild type (wt) or mutants (mt).
FIG. 4B is a graphical representation showing the intensity changes of the complex bands (measured by densitometry) as CR2 concentration increases from lanes 2 to 4 (wt), or lanes 6 to 8 (mt115), or lanes 10 to 12 (mt170) in FIG. 4A.
FIG. 4C is a graphical representation of a competitive ELISA demonstrating the relative abilities of wild type versus mutant forms of C3d to block CR2-wild type C3d interactions.
FIGS. 5A and 5B are two view with a 180 degree rotation to each other showing the localization of the epitopes of anti-CR2 monoclonal antibodies (mAb) on the CR2 surface.
FIG. 6A is a surface representation of the model containing a dimer of CR2 SCR1 and SCR2 that bind to C3d on each receptor.
FIG. 6B is a diagram of C3d-antigen cross-linking CR2 (as dimers) and BCR on the cell surface.



DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to the discovery of the three-dimensional structure of complement receptor 2 (CR2/CD21), to crystalline CR2-C3d complexes, to models of such three-dimensional structures, to a method of structure based drug design using such structures, to the compounds identified by such methods and to the use of such compounds in therapeutic compositions and methods. Complement receptor 2 (CR2/CD21) is an important receptor bridging the innate and adaptive immune systems that greatly amplifies B lymphocyte activation. CR2 ligands include complement C3d, C3, a CR-2 binding fragment of C3 that contains C3d or a portion thereof, CD23 and Epstein-Barr virus gp350/220. The structural basis for ligand binding by short consensus repeat (SCR) containing proteins has been unknown, but CR2 interactions require the presence of a two SCR-containing domain. In an effort to understand how CR2 interacts with its cellular ligand C3d in the process of B cell activation, as well as its other natural ligands, the present inventors have determined the 2 Å crystal structure of the CR2 SCR1 and SCR2 domain in complex with C3d. The present inventors describe herein the x-ray structure of this CR2 domain in complex with C3d, which reveals extensive main chain interactions of C3d with one SCR of CR2 and substantial SCR side-side packing. These results provide the first detailed understanding of receptor-ligand interactions in this protein family and reveal potential target sites for molecular drug design.
According to the present invention, the complement receptor 2 (CR2/CD21) is a protein that is characterized by the amino acid sequence represented by SEQ ID NO:1. SEQ ID NO:1 represents the full-length human CR2 protein sequence. The two short consensus repeat (SCR) domains of CR2 that are known to be required for CR2-ligand interactions, SCR1 and SCR2, are located, respectively, within the human CR2 amino acid sequence between positions Cys23 and Cys82 (i.e., between the first Cys and the fourth Cys residues) of SEQ ID NO:1 (SCR1, also represented herein by SEQ ID NO:2) and between positions Cys91 and Cys146 (i.e., between the fifth Cys and the eighth Cys residues) of SEQ ID NO:1 (SCR2, also represented herein by SEQ ID NO:3). The segment (fragment) of human CR2 represented in crystal structure herein contains both the SCR1 and the SCR2 domain (positions 20-153 of SEQ ID NO:1), and is represented herein by SEQ ID NO:4. SEQ ID NO:4 includes the 8 residue linker between SCR1 (SEQ ID NO:2) and SCR2 (SEQ ID NO:3). It also contains three residues at the N-terminus of SCR1 that match exactly positions Gly20-Ser22 of SEQ ID NO: 1. At the C-terminus of SCR2, SEQ ID NO:4 contains seven residues that match exactly positions Val147-Glu153 of SEQ ID NO:1.
The full-length mouse CR2 protein sequence is represented herein by SEQ ID NO:5. The SCR1 and SCR2 domains of the mouse CR2 protein are located with the mouse CR2 amino sequence at positions 14-73 of SEQ ID NO:5 (SCR1) and positions 82-138 of SEQ ID NO:5 (SCR2). The segment (fragment) of mouse CR2 that contains both the SCR1 and SCR2 domains and the eight residue linker, and which is shown aligned with the human sequence in FIG. 2E, is located at positions 11-145 of SEQ ID NO:5 and is represented herein by SEQ ID NO:6. Human and mouse CR2 are approximately 66% identical over the full length amino acid sequences represented by SEQ ID NO:1 and SEQ ID NO:5 (using BLAST 2 pairwise alignment), and approximately 61% identical over the SCR1-SCR2 regions of SEQ ID NO:4 and SEQ ID NO:6 (using BLAST 2 pairwise alignment). It is noted that both mouse and human CR2 bind to C3 (in the C3d region).
According to the present invention, general reference to a complement receptor 2 (CR2/CD21) protein is a protein that, at a minimum, contains any portion of the SCR1 and SCR2 domains of a CR2 protein, and includes full-length CR2 proteins, soluble CR2 proteins, other biologically active fragments of CR2 proteins, CR2 proteins comprising SCR1 and SCR2, CR2 fusion proteins, or any homologue of a naturally occurring CR2, as described in detail below. A homologue of a CR2 protein includes proteins which differ from a naturally occurring CR2 in that at least one or a few, but not limited to one or a few, amino acids have been deleted (e.g., a truncated version of the protein, such as a peptide or fragment), inserted, inverted, substituted and/or derivatized (e.g., by glycosylation, phosphorylation, acetylation, myristoylation, prenylation, palmitation, amidation and/or addition of glycosylphosphatidyl inositol). Preferably, a CR2 homologue has an amino acid sequence that is at least about 70% identical to the amino acid sequence of a naturally occurring CR2 (e.g., SEQ ID NO:1, or SEQ ID NO:5), and more preferably, at least about 75%, and more preferably, at least about 80%, and more preferably, at least about 85%, and more preferably, at least about 90%, and more preferably, at least about 95% identical to the amino acid sequence of a naturally occurring CR2. Preferred three-dimensional structural homologues of a CR2 are described in detail below. According to the present invention, a CR2 homologue preferably has, at a minimum, the ability to bind to a naturally occurring ligand of CR2 (e.g., C3d (including any C3 fragments with CR2-binding ability), CD23, EBV). Such homologues include fragments of a full length CR2 (e.g., the SCR2 region or the SCR1-SCR2 region) and can be referred to herein as a CR2 ligand-binding fragment. In one embodiment, a CR2 homologue has the biological activity of a naturally occurring CR2. Reference to a CR2 protein can also generally refer to CR2 in complex with a ligand.
In general, the biological activity or biological action of a protein refers to any function(s) exhibited or performed by the protein that is ascribed to the naturally occurring form of the protein as measured or observed in vivo (i.e., in the natural physiological environment of the protein) or in vitro (i.e., under laboratory conditions). Modifications of a protein, such as in a homologue or mimetic (discussed below), may result in proteins having the same biological activity as the naturally occurring protein, or in proteins having decreased or increased biological activity as compared to the naturally occurring protein. Modifications which result in a decrease in protein expression or a decrease in the activity of the protein, can be referred to as inactivation (complete or partial), down-regulation, or decreased action of a protein. Similarly, modifications which result in an increase in protein expression or an increase in the activity of the protein, can be referred to as amplification, overproduction, activation, enhancement, up-regulation or increased action of a protein. As used herein, a protein that has “CR2 biological activity” or that is referred to as a CR2 refers to a protein that has an activity that can include any one, and preferably more than one, of the following characteristics: (a) binds to a natural ligand of CR2 (e.g., C3d, EBV, CD23, C3 or other CR2-binding C3 fragments); (b) mediates interactions between the natural ligands and other proteins; (c) responds to contact with a natural ligand or other agonist (i.e., stimulation) by activation of the signal transduction cascade through the CR2/CD19/CD81 co-activation complex in a cell expressing such complex (D. T. Fearon, 1995 ibid.; D. T. Fearon, 1998, ibid.; J. C. Cambier, 1997, ibid; A. K. Matsumoto, et al., J Exp Med 173, 55-s 64 (1991)), including activation of lyn tyrosine kinase, activations of phosphatidyl inositol 3′ kinase, activation of NF-κB, activation of MAP kinases, phosphorylation of CD19, activation of PI3 kinase, and activation of protein kinase C (PKC). Such biological activities of (c) associated with the binding and activation of CR2 can be referred to as downstream biological activities, since they occur downstream of the binding of CR2 by its ligand.
An isolated protein (e.g., an isolated CR2 protein or an isolated C3d protein, an isolated C3 protein, or other CR2-binding C3 fragment), according to the present invention, is a protein that has been removed from its natural milieu (i.e., that has been subject to human manipulation) and can include purified proteins, partially purified proteins, recombinantly produced proteins, and synthetically produced proteins, for example. As such, “isolated” does not reflect the extent to which the protein has been purified. Preferably, an isolated protein, and particularly, an isolated CR2 protein and/or an isolated C3d protein or other CR2-binding C3 fragment, is produced recombinantly. According to the present invention, a CR2-binding C3 fragment can include any portion of C3 that contains at least a portion of C3d sufficient to bind to CR2, and can include, but is not limited to, portions of C3 comprising C3dg, iC3b, and/or C3b, an isolated C3d segment or a portion thereof. The terms “fragment”, “segment” and “portion” can be used interchangeably herein with regard to referencing a part of a protein.
Reference to a protein from a specific organism, such as a “human CR2”, by way of example, refers to a CR2 (including a homologue of a naturally occurring CR2) from a human or a CR2 protein that has been otherwise produced from the knowledge of the primary structure (e.g., sequence) and/or the tertiary structure of a naturally occurring CR2 protein from a human. In other words, a human CR2 protein includes any CR2 protein that has the structure and function of a naturally occurring CR2 protein from a human or that has a structure and function that is sufficiently similar to a human CR2 protein such that the CR2 protein is a biologically active (i.e., has biological activity) homologue of a naturally occurring CR2 protein from a human. As such, a human CR2 protein can include purified, partially purified, recombinant, mutated/modified and synthetic proteins.
Proteins of the present invention are preferably retrieved, obtained, and/or used in “substantially pure” form. As used herein, “substantially pure” refers to a purity that allows for the effective use of the protein in vitro, ex vivo or in vivo according to the present invention. For a protein to be useful in an in vitro, ex vivo or in vivo method according to the present invention, it is substantially free of contaminants, other proteins and/or chemicals that might interfere or that would interfere with its use in a method disclosed by the present invention, or that at least would be undesirable for inclusion with the protein when it is used in a method disclosed by the present invention. For example, for a CR2 protein, such methods include crystallization of the protein, use of a portion of the protein as a drug delivery vehicle, antibody production, agonist/antagonist identification assays, and all other methods disclosed herein. Preferably, a “substantially pure” protein, as referenced herein, is a protein that can be produced by any method (i.e., by direct purification from a natural source, recombinantly, or synthetically), and that has been purified from other protein components such that the protein comprises at least about 80% weight/weight of the total protein in a given composition (e.g., the protein is about 80% of the protein in a solution/composition/buffer), and more preferably, at least about 85%, and more preferably at least about 90%, and more preferably at least about 91%, and more preferably at least about 92%, and more preferably at least about 93%, and more preferably at least about 94%, and more preferably at least about 95%, and more preferably at least about 96%, and more preferably at least about 97%, and more preferably at least about 98%, and more preferably at least about 99%, weight/weight of the total protein in a given composition.
As used herein, a “structure” of a protein refers to the components and the manner of arrangement of the components to constitute the protein. The “three dimensional structure” or “tertiary structure” of the protein refers to the arrangement of the components of the protein in three dimensions. Such term is well known to those of skill in the art. It is also to be noted that the terms “tertiary” and “three dimensional” can be used interchangeably.
The present invention provides the atomic coordinates that define the three dimensional structure of a CR2 protein in complex with a C3d protein. A CR2-ligand complex, such as a CR2-C3d complex, refers to the complex (e.g., interaction, binding), that to forms between CR2 and any of its ligands (e.g., C3d) in the absence of a compound that interferes with the interaction between the CR2 and its ligand(s). A complex is naturally formed between at least one full length CR2 and a full length ligand, but according to the present invention, a CR2-ligand can also include complexes that minimally contain: (1) a CR2 SCR1 and/or CR2 SCR2 domain; and (2) a CR2-contacting portion of a ligand of CR2.
One embodiment of the present invention includes a CR2 protein in crystalline form. The present invention specifically exemplifies a portion of CR2 comprising the SCR1 and SCR2 domains. As used herein, the terms “crystalline CR2” and “CR2 crystal” both refer to crystallized CR2 protein and are intended to be used interchangeably. Preferably, a crystalline CR2 is produced using the crystal formation method described herein, in particular according to the method disclosed in Example 1. A CR2 crystal of the present invention can comprise any crystal structure and preferably crystallizes as an orthorhombic crystal lattice. A suitable crystalline CR2 of the present invention includes a monomer or a dimer, or a multimer of CR2 protein. One preferred crystalline CR2 comprises between one and five CR2 proteins in an asymmetric unit. A more preferred crystalline CR2 comprises a dimer of CR2 proteins. Preferably, a composition of the present invention includes CR2 protein molecules arranged in a crystalline manner in a space group R3 or R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å. A preferred crystal of the present invention provides X-ray diffraction data for determination of atomic coordinates of the CR2 protein to a resolution of about 4.0 Å, and preferably to about 3.0 Å, and more preferably to about 2.0 Å.
One embodiment of the present invention includes a method for producing crystals of CR2, alone or in complex with a CR2 ligand, comprising combining CR2 protein with a mother liquor and inducing crystal formation to produce the CR2 crystals. Although the production of crystals of CR2 in complex with C3d are specifically described herein, it is to be understood that such processes as are described herein can be adapted by those of skill in the art to produce crystals of CR2 in complex with other CR2 ligands, such as Epstein Barr Virus (EBV) or CD23.
By way of example, crystals of CR2 and C3d in complex are formed using a solution containing about 20 mg/ml of CR2-C3d complex in a mother liquor. A suitable mother liquor of the present invention comprises an acetate buffer or a sulfate buffer. A preferred acetate buffer of the present invention comprises zinc acetate or zinc sulfate. The concentration of ammonium acetate in the buffer prior to crystallization is preferably 0.2M. The pH of the acetate buffer (pH 7.36) is controlled using 0.1 M NaCacodylate. The acetate buffer also contains any polyethylene glycol (PEG), with PEG 2000 at a concentration of about 17% being more preferred. Supersaturated solutions of CR2-C3d complex can be induced to crystallize by several methods including, but not limited to, vapor diffusion, liquid diffusion, batch crystallization, constant temperature and temperature induction or a combination thereof. Preferably, supersaturated solutions of CR2-C3d complex are induced to crystallize by hanging drop vapor diffusion. In a vapor diffusion method, a CR2-C3d complex is combined with a mother liquor of the present invention that will cause the CR2-C3d complex solution to become supersaturated and form CR2-C3d complex crystals at a constant temperature. Vapor diffusion is preferably performed under a controlled temperature and, by way of example, can be performed at 4° C.
One embodiment of the present invention includes a representation, or model, of the three dimensional structure of a CR2 protein, such as a computer model. A computer model of the present invention can be produced using any suitable software program, including, but not limited to, MOLSCRIPT 2.0 (Avatar Software AB, Heleneborgsgatan 21C, SE-11731 Stockholm, Sweden), the graphical display program 0 (Jones et. al., Acta Crystallography, vol. A47, p. 110, 1991), the graphical display program GRASP, or the graphical display program INSIGHT. Suitable computer hardware useful for producing an image of the present invention are known to those of skill in the art (e.g., a Silicon Graphics Workstation).
A representation, or model, of the three dimensional structure of the CR2-C3d complex structure for which a crystal has been produced can also be determined using techniques which include molecular replacement or SIR/MIR (single/multiple isomorphous replacement). Methods of molecular replacement are generally known by those of skill in the art (generally described in Brunger, Meth. Enzym., vol. 276, pp. 558-580, 1997; Navaza and Saludjian, Meth. Enzym., vol. 276, pp. 581-594, 1997; Tong and Rossmann, Meth. Enzym., vol. 276, pp. 594-611, 1997; and Bentley, Meth. Enzym., vol. 276, pp. 611-619, 1997, each of which are incorporated by this reference herein in their entirety) and are performed in a software program including, for example, AmoRe (CCP4, Acta Cryst. D50, 760-763 (1994) or XPLOR. Briefly, X-ray diffraction data is collected from the crystal of a crystallized target structure. The X-ray diffraction data is transformed to calculate a Patterson function. The Patterson function of the crystallized target structure is compared with a Patterson function calculated from a known structure (referred to herein as a search structure). The Patterson function of the crystallized target structure is rotated on the search structure Patterson function to determine the correct orientation of the crystallized target structure in the crystal. The translation function is then calculated to determine the location of the target structure with respect to the crystal axes. Once the crystallized target structure has been correctly positioned in the unit cell, initial phases for the experimental data can be calculated. These phases are necessary for calculation of an electron density map from which structural differences can be observed and for refinement of the structure. Preferably, the structural features (e.g., amino acid sequence, conserved di-sulphide bonds, and β-strands or β-sheets) of the search molecule are related to the crystallized target structure.
As used herein, the term “model” refers to a representation in a tangible medium of the three dimensional structure of a protein, polypeptide or peptide. For example, a model can be a representation of the three dimensional structure in an electronic file, on a computer screen, on a piece of paper (i.e., on a two dimensional medium), and/or as a ball-and-stick figure. Physical three-dimensional models are tangible and include, but are not limited to, stick models and space-filling models. The phrase “imaging the model on a computer screen” refers to the ability to express (or represent) and manipulate the model on a computer screen using appropriate computer hardware and software technology known to those skilled in the art. Such technology is available from a variety of sources including, for example, Evans and Sutherland, Salt Lake City, Utah, and Biosym Technologies, San Diego, Calif. The phrase “providing a picture of the model” refers to the ability to generate a “hard copy” of the model. Hard copies include both motion and still pictures. Computer screen images and pictures of the model can be visualized in a number of formats including space-filling representations, α carbon traces, ribbon diagrams and electron density maps.
Preferably, a three dimensional structure of a CR2 protein provided by the present invention includes: (a) a structure defined by atomic coordinates of a three dimensional structure of a crystalline CR2 SCR1-2 region in complex with C3d; (b) a structure defined by atomic coordinates selected from the group consisting of: (i) atomic coordinates represented in a table selected from the group consisting of Table 2 (CR2-C3d) and Table 3 (CR2 only); and, (ii) atomic coordinates that define a three dimensional structure, wherein at least 50% of the structure has an average root-mean-square deviation (RMSD) from backbone atoms in secondary structure elements in at least one domain of a three dimensional structure represented by the atomic coordinates of (1) of equal to or less than about 1.0 Å; and/or (c) a structure defined by atomic coordinates derived from CR2 protein molecules arranged in a crystalline manner in a space group R3 or R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å.
The present inventors have provided the atomic coordinates that define the three dimensional structure of a crystalline CR2 short consensus repeat (SCR) 1-2 region (CR2 SCR1-2 region) in complex with C3d. Using the guidance provided herein, one of skill in the art will be able to reproduce such a crystalline structure and define atomic coordinates of such a structure. Example 1 demonstrates the production of a CR2-C3d complex (CR2 SCR1-2 region in complex with C3d) arranged in a crystalline manner in a space group R3 or R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å. The atomic coordinates determined from this crystal structure are represented in Table 2. Additionally, these atomic coordinates were deposited on Jan. 11, 2001, with the Protein Data Bank (PDB), operated by the Research Collaboratory for Structural Bioinformatics (RCSB) (H. M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T. N. Bhat, H. Weissig, I. N. Shindyalov, P. E. Bourne, The Protein Data Bank; Nucleic Acids Research, 28:235-242 (2000)), under PDB Deposit No. PDB id 1 GHQ. The atomic coordinates in Table 3 are the coordinates that define the three dimensional structure of just the CR2 SCR1-SCR2 domains of the CR2-C3d complex (i.e., the coordinates defining the C3d portion have been removed).
In one embodiment, a three dimensional structure of a CR2 protein provided by the present invention includes a structure represented by atomic coordinates that define a three dimensional structure, wherein at least 50% of the structure has an average root-mean-square deviation (RMSD) from backbone atoms in secondary structure elements in at least one domain of a three dimensional structure represented by the atomic coordinates of Table 2 or Table 3 of equal to or less than about 1.0 Å. Such a structure can be referred to as a structural homologue of the CR2 structures defined by Tables 2 and 3. Preferably, at least 50% of the structure has an average root-mean-square deviation (RMSD) from backbone atoms in secondary structure elements in at least one domain of a three dimensional structure represented by the atomic coordinates of Table 2 or Table 3 of equal to or less than about 0.7 Å, equal to or less than about 0.5 Å, and most preferably, equal to or less than about 0.3 Å. In a more preferred embodiment, a three dimensional structure of a CR2 protein provided by the present invention includes a structure defined by atomic coordinates that define a three dimensional structure, wherein at least about 75% of such structure has the recited average root-mean-square deviation (RMSD) value, and more preferably, at least about 90% of such structure has the recited average root-mean-square deviation (RMSD) value, and most preferably, about 100% of such structure has the recited average root-mean-square deviation (RMSD) value.
In one embodiment, RMSD of a structural homologue of CR2 can be extended to include atoms of amino acid side chains. As used herein, the phrase “common amino acid side chains” refers to amino acid side chains that are common to both the structural homologue and to the structure that is actually represented by such atomic coordinates. Preferably, at least 50% of the structure has an average root-mean-square deviation (RMSD) from common amino acid side chains in at least one domain of a three dimensional structure represented by the atomic coordinates of Table 2 or Table 3 of equal to or less than about 1.0 Å equal to or less than about 0.7 Å, equal to or less than about 0.5 Å, and most preferably, equal to or less than about 0.3 Å. In a more preferred embodiment, a three dimensional structure of a CR2 protein provided by the present invention includes a structure defined by atomic coordinates that define a three dimensional structure, wherein at least about 75% of such structure has the recited average root-mean-square deviation (RMSD) value, and more preferably, at least about 90% of such structure has the recited average root-mean-square deviation (RMSD) value, and most preferably, about 100% of such structure has the recited average root-mean-square deviation (RMSD) value.
One embodiment of the present invention relates to a method of structure-based identification of compounds which potentially bind to complement receptor type 2 (CR2) proteins or to a complex of CR2 and its ligand, comprising: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region; and (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region. The three dimensional structure of the CR2 SCR 1-2 region is selected from the group of:

    
    
        (i) a structure defined by atomic coordinates of a three dimensional structure of a crystalline CR2 SCR1-2 region in complex with C3d;
        (ii) a structure defined by atomic coordinates selected from the group consisting of:
        
            (1) atomic coordinates represented in a table selected from the group consisting of Table 2 (CR2-C3d) and Table 3 (CR2 only);
            (2) atomic coordinates that define a three dimensional structure, wherein at least 50% of the structure has an average root-mean-square deviation (RMSD) from backbone atoms in secondary structure elements in at least one domain of a three dimensional structure represented by the atomic coordinates of (1) of equal to or less than about 1.0 Å; and
        
        
        (iii) a structure defined by atomic coordinates derived from CR2 protein molecules arranged in a crystalline manner in a space group R3 or R32 so as to form a unit cell of dimensions a=b=170.5 Å, c=173.8 Å.
    
    


The structures used to perform the above-described method have been described in detail above and in the Examples section. According to the present invention, the phrase “providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region” is defined as any means of providing, supplying, accessing, displaying, retrieving, or otherwise making available the three dimensional structure of the CR2 short consensus repeat (SCR) 1-2 region described herein. For example, the step of providing can include, but is not limited to, accessing the atomic coordinates for the structure from a database; importing the atomic coordinates for the structure into a computer or other database; displaying the atomic coordinates and/or a model of the structure in any manner, such as on a computer, on paper, etc.; and determining the three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region de novo using the guidance provided herein.
The second step of the method of structure based identification of compounds of the present invention includes identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region. CR2 is a receptor for at least three biologically important ligands, and has been shown to play a role in several aspects of the humoral immune response, EBV infection, and HIV-1 infection. Therefore, identification and/or design of compounds that mimic, enhance, disrupt or compete with the interactions of CR2 with its ligands are highly desirable. Such compounds can be designed using structure based drug design. Until the discovery of the three dimensional structure of the present invention, the only information available for the development of therapeutic compounds based on the CR2 protein was based on the primary sequence of the CR2 protein. Structure based drug design refers to the prediction of a conformation of a peptide, polypeptide, protein, or conformational interaction between a peptide or polypeptide, and a compound, using the three dimensional structure of the peptide, polypeptide or protein. Typically, structure based drug design is performed with a computer. For example, generally, for a protein to effectively interact with (e.g., bind to) a compound, it is necessary that the three dimensional structure of the compound assume a compatible conformation that allows the compound to bind to the protein in such a manner that a desired result is obtained upon binding. Knowledge of the three dimensional structure of the protein enables a skilled artisan to design a compound having such compatible conformation, or to select such a compound from available libraries of compounds. For example, knowledge of the three dimensional structure of the C3d binding site of CR2 enables one of skill in the art to design a compound that binds to CR2, is stable and results in, for example, inhibition of a biological response such as C3d binding to CR2, or cellular signal transduction through the CR2, upon such binding. In addition, for example, knowledge of the three dimensional structure of the C3d binding site of a CR2 enables a skilled artisan to design a substrate analog of CR2.
Suitable structures and models useful for structure based drug design are disclosed herein. Preferred target structures to use in a method of structure based drug design include any representations of structures produced by any modeling method disclosed herein, including molecular replacement and fold recognition related methods.
According to the present invention, the step of designing a compound for testing in a method of structure based identification of the present invention can include creating a new chemical compound or searching databases of libraries of known compounds (e.g., a compound listed in a computational screening database containing three dimensional structures of known compounds). Designing can also be performed by simulating chemical compounds having substitute moieties at certain structural features. The step of designing can include selecting a chemical compound based on a known function of the compound. A preferred step of designing comprises computational screening of one or more databases of compounds in which the three dimensional structure of the compound is known and is interacted (e.g., docked, aligned, matched, interfaced) with the three dimensional structure of a CR2 by computer (e.g. as described by Humblet and Dunbar, Animal Reports in Medicinal Chemistry, vol. 28, pp. 275-283, 1993, M Venuti, ed., Academic Press). Methods to synthesize suitable chemical compounds are known to those of skill in the art and depend upon the structure of the chemical being synthesized. Methods to evaluate the bioactivity of the synthesized compound depend upon the bioactivity of the compound (e.g., inhibitory or stimulatory) and are disclosed herein.
Various other methods of structure-based drug design are disclosed in Maulik et al., 1997, Molecular Biotechnology: Therapeutic Applications and Strategies, Wiley-Liss, Inc., which is incorporated herein by reference in its entirety. Maulik et al. disclose, for example, methods of directed design, in which the user directs the process of creating novel molecules from a fragment library of appropriately selected fragments; random design, in which the user uses a genetic or other algorithm to randomly mutate fragments and their combinations while simultaneously applying a selection criterion to evaluate the fitness of candidate ligands; and a grid-based approach in which the user calculates the interaction energy between three dimensional receptor structures and small fragment probes, followed by linking together of favorable probe sites.
In a molecular diversity strategy, large compound libraries are synthesized, for example, from peptides, oligonucleotides, carbohydrates and/or synthetic organic molecules, using biological, enzymatic and/or chemical approaches. The critical parameters in developing a molecular diversity strategy include subunit diversity, molecular size, and library diversity. The general goal of screening such libraries is to utilize sequential application of combinatorial selection to obtain high-affinity ligands for a desired target, and then to optimize the lead molecules by either random or directed design strategies. Methods of molecular diversity are described in detail in Maulik, et al., ibid.
Maulik et al. also disclose, for example, methods of directed design, in which the user directs the process of creating novel molecules from a fragment library of appropriately selected fragments; random design, in which the user uses a genetic or other algorithm to randomly mutate fragments and their combinations while simultaneously applying a selection criterion to evaluate the fitness of candidate ligands; and a grid-based approach in which the user calculates the interaction energy between three dimensional receptor structures and small fragment probes, followed by linking together of favorable probe sites.
In the present method of structure based drug design, it is not necessary to align a candidate chemical compound (i.e., a chemical compound being analyzed in, for example, a computational screening method of the present invention) to each residue in a target site (target sites will be discussed in detail below). Suitable candidate chemical compounds can align to a subset of residues described for a target site. Preferably, a candidate chemical compound comprises a conformation that promotes the formation of covalent or noncovalent crosslinking between the target site and the candidate chemical compound. Preferably, a candidate chemical compound binds to a surface adjacent to a target site to provide an additional site of interaction in a complex. When designing an antagonist (i.e., a chemical compound that inhibits the binding of a ligand to CR2 by blocking a binding site or interface), for example, the antagonist should bind with sufficient affinity to the binding site or to substantially prohibit a ligand (i.e., a molecule that specifically binds to the target site) from binding to a target area. It will be appreciated by one of skill in the art that it is not necessary that the complementarity between a candidate chemical compound and a target site extend over all residues specified here in order to inhibit or promote binding of a ligand.
In general, the design of a chemical compound possessing stereochemical complementarity can be accomplished by techniques that optimize, chemically or geometrically, the “fit” between a chemical compound and a target site. Such techniques are disclosed by, for example, Sheridan and Venkataraghavan, Acc. Chem Res., vol. 20, p. 322, 1987: Goodford, J. Med. Chem., vol. 27, p. 557, 1984; Beddell, Chem. Soc. Reviews, vol. 279, 1985; Hol, Angew. Chem., vol. 25, p. 767, 1986; and Verlinde and Hol, Structure, vol. 2, p. 577, 1994, each of which are incorporated by this reference herein in their entirety.
One embodiment of the present invention for structure based drug design comprises identifying a chemical compound that complements the shape of a CR2, including a portion of CR2, such as the SCR1-SCR2 region. Such method is referred to herein as a “geometric approach”. In a geometric approach, the number of internal degrees of freedom (and the corresponding local minima in the molecular conformation space) is reduced by considering only the geometric (hard-sphere) interactions of two rigid bodies, where one body (the active site) contains “pockets” or “grooves” that form binding sites for the second body (the complementing molecule, such as a ligand).
The geometric approach is described by Kuntz et al., J. Mol. Biol., vol. 161, p. 269, 1982, which is incorporated by this reference herein in its entirety. The algorithm for chemical compound design can be implemented using the software program DOCK Package, Version 1.0 (available from the Regents of the University of California). Pursuant to the Kuntz algorithm, the shape of the cavity or groove on the surface of a structure (e.g., CR2) at a binding site or interface is defined as a series of overlapping spheres of different radii. One or more extant databases of crystallographic data (e.g., the Cambridge Structural Database System maintained by University Chemical Laboratory, Cambridge University, Lensfield Road, Cambridge CB2 1EW, U.K.) or the Protein Data Bank maintained by Brookhaven National Laboratory, is then searched for chemical compounds that approximate the shape thus defined.
Chemical compounds identified by the geometric approach can be modified to satisfy criteria associated with chemical complementarity, such as hydrogen bonding, ionic interactions or Van der Waals interactions.
Another embodiment of the present invention for structure based identification of compounds comprises determining the interaction of chemical groups (“probes”) with an active site at sample positions within and around a binding site or interface, resulting in an array of energy values from which three dimensional contour surfaces at selected energy levels can be generated. This method is referred to herein as a “chemical-probe approach.” The chemical-probe approach to the design of a chemical compound of the present invention is described by, for example, Goodford, J. Med. Chem., vol. 28, p. 849, 1985, which is incorporated by this reference herein in its entirety, and is implemented using an appropriate software package, including for example, GRID (available from Molecular Discovery Ltd., Oxford OX2 9LL, U.K.). The chemical prerequisites for a site-complementing molecule can be identified at the outset, by probing the active site of a CR2, for example, (as represented by the atomic coordinates shown in Table 2 or Table 3) with different chemical probes, e.g., water, a methyl group, an amine nitrogen, a carboxyl oxygen and/or a hydroxyl. Preferred sites for interaction between an active site and a probe are determined. Putative complementary chemical compounds can be generated using the resulting three dimensional pattern of such sites.
According to the present invention, suitable candidate compounds to test using the method of the present invention include proteins, peptides or other organic molecules, and inorganic molecules. Suitable organic molecules include small organic molecules. Peptides refer to small molecular weight compounds yielding two or more amino acids upon hydrolysis. A polypeptide is comprised of two or more peptides. As used herein, a protein is comprised of one or more polypeptides. Preferred therapeutic compounds to design include peptides composed of “L” and/or “D” amino acids that are configured as normal or retroinverso peptides, peptidomimetic compounds, small organic molecules, or homo- or hetero-polymers thereof, in linear or branched configurations.
Preferably, a compound that is identified by the method of the present invention originates from a compound having chemical and/or stereochemical complementarity with CR2 and/or C3d. Such complementarity is characteristic of a compound that matches the surface of the receptor either in shape or in distribution of chemical groups and binds to CR2 to promote or inhibit CR2 ligand binding, or to induce cellular signal transduction in a cell expressing CR2 upon the binding of the compound to CR2. More preferably, a compound that binds to a ligand binding site of CR2 associates with an affinity of at least about 10−6 M, and more preferably with an affinity of at least about 10−7M, and more preferably with an affinity of at least about 10−8 M.
Preferably, three general sites of the CR2 are targets for structure based drug design (i.e., target sites), although other sites may become apparent to those of skill in the art. The three preferred sites include: (1) the interface between CR2 and C3d; (2) the interface between the SCR1 and SCR2 domains of CR2; and (3) the dimerization interface between two CR2 monomers. Combinations of any of these general sites are also suitable target sites. The interface between CR2 and C3d is depicted in FIG. 1A, and FIGS. 3A-3E. The interface between the SCR1 and SCR2 domains of CR2 is depicted in FIGS. 2A-2C. The dimer interface between CR2 monomeric proteins is depicted in FIGS. 1B, 2D and 6A. The following discussion provides specific detail on compound identification (e.g., drug design) using target sites of the CR2 based on its three-dimensional structure in complex with C3d. It is to be understood, however, that one of skill in the art, using the description of the CR2 structure provided herein, will be able to identify compounds that are potential candidates for inhibiting, stimulating or enhancing the interaction of CR2 with its other ligands.
The C3d binding site (i.e., the interface between CR2 and C3d) is targeted to directly affect the binding of CR2 to C3d, other CR2-binding C3 fragments, or another ligand (i.e., inhibition or enhancement). In the CR2-C3d complex, no continuous stretch of residues on C3d participates in the interactions. Rather, residues that are separated in the linear sequence of C3d, but come together on the folded C3d, interact with CR2. Namely, the residues on the H3-H4 loop (the loop between helix 3 and 4), as well as H5, and H7 make contact with CR2 (FIG. 3C). On the CR2 part, however, a linear stretch of residues within SCR2 domain makes the contact with C3d. The B strand and B-C loop of SCR2 constitute the majority of the interactions with C3d. The nature of the contacts involves elegant networks of hydrogen bonds, plus some hydrophobic and van der Waals interactions (FIGS. 3D & 3E). The contact site is discussed in detail in the Examples. Preferred target sites in the C3d binding interface with CR2 include, but are not limited to: (1) a site on the B strand and the B-C loop of CR2 SCR2 comprising the segment: G79-G80-Y81-K82-183-R84-G85-S86-T87-P88-Y89; (2) a site on the B strand of CR2 SCR2 comprising position K100; (3) a segment of CR2 SCR2 comprising V130-F131-P132-L133; and (4) a segment of CR2 SCR2 comprising the fragment T101-N102-F103. In the T101-N102-F103 site, the N102 residue is glycosylated. The N-acetylglucosamine residue attached to N102 forms a hydrogen bond with C3d. The present inventors have shown that amino acid residues at positions 84 and 86 of SEQ ID NO:4 are particularly important residues in the binding of C3d to CR2 and therefore, segments of CR2 including these residues are particularly desirable target sites. Alternatively, the CR2 contact points on C3d can be targeted for the identification of compounds that specifically modify the interaction between CR2 and C3d (and potentially the interaction of CR2 with its other ligands). Such sites include, but are not limited to: (1) the loop between helix 2-3 of C3d comprising the segment Q68-P69-S70-S71; (2) Helix 5 of C3d comprising the segment S104-Q105-V106-L107-C108-G109-A110-V111-K112-W113-L114-I115-L116-E117-K11-8-Q119-K120-P121-D122; and (3) Helix 7 of C3d comprising the segment N170-S171-L172-P173-G174-S175-I176-T177-K178-A179-G180-D181-F182-L183-E18-4-A185 (all positions are given with respect to SEQ ID NO:7).
The interface between the two monomers of CR2 can also be targeted to affect the binding of CR2 to a ligand. Two CR2 molecules dimerize through SCR1-SCR1 contacts in the crystal structure (FIG. 2D). The contact is symmetrical, with the E1 strands from different molecules running anti-parallel to each other. The C-terminus of the E1 strand also contacts the C-terminus of the D2 strand of another molecule. The nature of the interaction is hydrogen bonds. Without being bound by theory, the present inventors believe that a CR2 dimer, in contrast to a monomer, can allow a dramatic increase of cross-linked CR2/CD19/CD81 and B cell receptor molecules through binding to the antigen with two or more C3d attached, which can cause a 10.000-fold enhancement of the humoral immune response. Therefore, compounds that disrupt the dimer formation are expected to be primarily inhibitory compounds with regard to immune responses. Such compounds may also be beneficial, however, in inhibiting EBV or HIV infection mediated by CR2. Similarly, compounds that enhance or stabilize the dimer formation are expected to stimulate or enhance an immune response.
The interface between the SCR1 and SCR2 domains of CR2 is also targeted to affect the binding of CR2 to a ligand (i.e., inhibit or enhance). The packing of the two SCR domains in CR2 forms a V shape. Residues important for the tight packing between the two domains at the interface and the linker regions are shown in FIG. 2B. A very unique feature of the two domain CR2 structure is that the 8 amino acid linker makes a dramatic turn to allow the two SCR domains to pack against each other sideways (FIGS. 2A, 2B, 2C). Among the protein family containing SCR domains, the linkers connecting SCR domains are normally 3-5 amino acids in length, while an 8 amino acid linker is the longest known so far. The available structures of four SCR proteins containing two or 4 repeats (A. P. Wiles, et al., (1997), supra; P. N. Barlow, et al., (1993), supra; J. M. Casasnovas, et al., (1999), supra; R. Schwarzenbacher et al., (1999), supra) all have end-end packing between consecutive SCRs, and CR2 is thus the first to demonstrate extensive side-side packing. In end-end packing, the adjacent domains could, in principle, adopt different rotations and bend angles relative to each other in different environments. Indeed, a commonly held concept is that SCRs are not absolutely fixed relative to each other but rather are allowed some freedom to move about this interface (P. N. Barlow, et al., (1993), ibid.; J. M. Casasnovas, et al., (1999), ibid.). However, the side-side packing of the CR2 SCR1 and SCR2 would not give the two domains freedom to adopt different orientations, unless some active process is involved to first separate the two domains. The interface between the two domains is mainly hydrophobic (FIG. 2B). Trp112, which is unique to CR2 SCR2 sequence and located on strand D2, plays a critical role in the packing by interacting with Ile39 and the main-chain from SCR1. Trp 112 would be unfavorably exposed to the solvent if the two domains do not pack against each other sideways in this manner. In addition to Trp 112, several other residues also play a role in the packing, including Pro 121, His91, Leu39, and the carbon side chain of Glu64. The 8 amino acid linker, which contains mainly hydrophobic residues such as Tyr65, Phe66, Tyr69, also participates in the hydrophobic packing outside the two-domain interface, further solidifying the interactions between SCR1 and SCR2 (FIG. 2B). The area of the interface of the two SCRs is very extensive, covering almost half of the length of one SCR domain (FIG. 2C). Since the above-mentioned previous studies of other SCR proteins were performed in the absence of their ligands, it is possible that this interface may change upon binding of CR2 to its ligand. In any event, it is predicted that the binding of a compound to this site will have an effect on the ligand-binding ability of CR2.
A candidate compound for binding to a CR2 protein, including to one of the preferred target sites described above, is identified by one or more of the methods of structure-based identification discussed above. As used herein, a “candidate compound” refers to a compound that is selected by a method of structure-based identification described herein as having a potential for binding to a CR2 protein (or its ligand) on the basis of a predicted conformational interaction between the candidate compound and the target site of the CR2 protein. The ability of the candidate compound to actually bind to a CR2 protein can be determined using techniques known in the art, as discussed in some detail below. A “putative compound” is a compound with an unknown regulatory activity, at least with respect to the ability of such a compound to bind to and/or regulate CR2 as described herein. Therefore, a library of putative compounds can be screened using structure based identification methods as discussed herein, and from the putative compounds, one or more candidate compounds for binding to CR2 can be identified. Alternatively, a candidate compound for binding to CR2 can be designed de novo using structure based drug design, also as discussed above. Candidate compounds can be selected based on their predicted ability to inhibit the binding of CR2 to its ligand, to stabilize (e.g., enhance) the binding of CR2 to its ligand, to bind to and activate CR2, to bind to and inhibit the activation of CR2, to bind to and activate a ligand of CR2, to bind to and inhibit the activation of a ligand of CR2, to disrupt the dimerization of CR2 monomers, or to stabilize the dimerization of CR2 monomers.
Accordingly, in one aspect of the present invention, the method of structure-based identification of compounds that potentially bind to complement receptor type 2 (CR2) proteins or to a complex of CR2 and its ligand further includes steps which confirm whether or not a candidate compound has the predicted properties with respect to its effect on CR2 (or a ligand of CR2). In one embodiment, the candidate compound is predicted to be an inhibitor of the binding of CR2 to its ligand, and the method further includes: (c) contacting the candidate compound identified in step (b) with CR2 or a fragment thereof and a CR2 ligand or a fragment thereof under conditions in which a CR2-CR2 ligand complex can form in the absence of the candidate compound; and (d) measuring the binding affinity of the CR2 or fragment thereof to the CR2 ligand or fragment thereof. A candidate inhibitor compound is selected as a compound that inhibits the binding of CR2 to its ligand when there is a decrease in the binding affinity of the CR2 or fragment thereof for the CR2 ligand or fragment thereof, as compared to in the absence of the candidate inhibitor compound.
In another embodiment, the candidate compound is predicted to be a stabilizer of the binding of CR2 to its ligand, and the method further comprises: (c) contacting the candidate compound identified in step (b) with a CR2-CR2 ligand complex, wherein the CR2-CR2 ligand complex comprises CR2 or a fragment thereof and a CR2 ligand, or a fragment thereof; (d) measuring the stability of the CR2-CR2 ligand complex of (i). A candidate stabilizer compound is selected as a compound that stabilizes the CR2-CR2 ligand complex when there is an increase in the stability of the complex as compared to in the absence of the candidate stabilizer compound.
In another embodiment, the candidate compound is predicted to bind to and activate CR2 (i.e., an agonist), and the method further comprises: (c) contacting the candidate compound identified in step (b) with CR2 or a ligand-binding fragment thereof, under conditions wherein in the absence of the compound, CR2 is not activated; and, (d) measuring the ability of the candidate compound to bind to CR2 to activate CR2. A candidate agonist compound is selected as a compound that binds to CR2 and activates CR2 as compared to in the absence of the candidate agonist compound. A similar embodiment includes the identification of candidate compounds that bind to target sites on the CR2 ligand which are now known as a result of the present inventors' work, and the determination of the ability of the candidate compound to bind to and activate the ligand of CR2 (e.g., by mimicking the structure of CR2).
In another embodiment, the candidate compound is predicted to bind to and inhibit CR2 (i.e., an antagonist), and the method further comprises: (c) contacting the candidate compound identified in step (b) with CR2 or a ligand-binding fragment thereof, wherein in the absence of the compound, CR2 is not activated; and, (d) measuring the ability of the candidate compound to bind to CR2 and activate CR2. A candidate antagonist compound is selected as a compound that binds to CR2 but does not activate and, in some embodiments, inhibits any constitutive activation, of the CR2. A similar embodiment includes the identification of candidate compounds that bind to target sites on the CR2 ligand which are now known as a result of the present inventors' work, and the determination of the ability of the candidate compound to bind to but not activate the ligand of CR2.
In another embodiment, the candidate compound is predicted to bind to CR2 and to disrupt the dimerization of CR2 monomers, and the method further comprises: (c) contacting the candidate compound identified in step (b) with at least two CR2 monomers or ligand-binding fragments thereof, in the presence and in the absence of a CR2 ligand or fragment thereof; and, (d) measuring the ability of the candidate compound to bind to CR2, the ability of the CR2 monomers to dimerize, and/or the ability of the CR2 ligand to activate CR2. A candidate compound for the disruption of CR2 dimerization is selected as a compound that binds to CR2 but inhibits the dimerization of CR2 and in some embodiments, inhibits the activation of CR2 by its ligand. Similarly, a candidate compound for stabilizing the dimerization of CR2 is a compound that binds to CR2, prolongs the dimerization of CR2 as compared to in the absence of the candidate compound, and in some embodiments, enhances or prolongs the activation of CR2 by its ligand.
In one embodiment, the conditions under which a CR2 according to the present invention is contacted with a candidate compound, such as by mixing, are conditions in which the receptor is not stimulated (activated) or bound to a natural ligand if essentially no candidate compound is present. For example, such conditions include normal culture conditions in the absence of a stimulatory compound (a stimulatory compound being, e.g., the natural ligand for the receptor (e.g., C3d, CD23, EBV), a stimulatory antibody, or other equivalent stimulus). In this embodiment, the candidate compound is then contacted with the CR2. In this embodiment, the step of detecting is designed to indicate whether the candidate compound binds to CR2, and in some embodiments, whether the candidate compound activates CR2.
In an alternate embodiment, the conditions under which a CR2 according to the present invention is contacted with a candidate compound, such as by mixing, are conditions in which the receptor is normally bound by a ligand or additionally stimulated (activated) if essentially no candidate compound is present. Such conditions can include, for example, contact of CR2 with a stimulator molecule (a stimulatory compound being, e.g., the natural ligand for the receptor, a stimulatory antibody, or other equivalent stimulus) which binds to the receptor and causes the receptor to become activated. In this embodiment, the candidate compound can be contacted with the receptor prior to the contact of the receptor with the stimulatory compound (e.g., to determine whether the candidate compound blocks or otherwise inhibits the binding and/or stimulation of CR2 by the stimulatory compound), or after contact of the receptor with the stimulatory compound (e.g., to determine whether the candidate compound downregulates, or reduces the activation of the receptor).
The present methods involve contacting CR2 with the candidate compound being tested for a sufficient time to allow for binding to, activation or inhibition of the receptor by the candidate compound. The period of contact with the candidate compound being tested can be varied depending on the result being measured, and can be determined by one of skill in the art. For example, for binding assays, a shorter time of contact with the candidate compound being tested is typically suitable, than when activation is assessed. As used herein, the term “contact period” refers to the time period during which the CR2 molecules are in contact with the compound being tested. The term “incubation period” refers to the entire time during which cells expressing CR2, for example, are allowed to grow prior to evaluation, and can be inclusive of the contact period. Thus, the incubation period includes all of the contact period and may include a further time period during which the compound being tested is not present but during which growth is continuing (in the case of a cell based assay) prior to scoring. The incubation time for growth of cells can vary but is sufficient to allow for the binding of CR2, activation of the receptor or signal transduction pathways associated with the receptor, and/or inhibition of the receptor. It will be recognized that shorter incubation times are preferable because compounds can be more rapidly screened. A preferred incubation time is between about 1 minute to about 48 hours.
In accordance with the present invention, a cell-based assay is conducted under conditions which are effective to screen for candidate compounds useful in the method of the present invention. Effective conditions include, but are not limited to, appropriate media, temperature, pH and oxygen conditions that permit the growth of the cell that expresses the receptor. An appropriate, or effective, medium refers to any medium in which a cell that naturally or recombinantly expresses a CR2, when cultured, is capable of cell growth and expression of CR2. Such a medium is typically a solid or liquid medium comprising growth factors and assimilable carbon, nitrogen and phosphate sources, as well as appropriate salts, minerals, metals and other nutrients, such as vitamins. Culturing is carried out at a temperature, pH and oxygen content appropriate for the cell. Such culturing conditions are within the expertise of one of ordinary skill in the art.
Cells that are useful in the cell-based assays of the present invention include any cell that expresses a CR2 and particularly, other proteins that are associated with CR2 signal transduction cascades (e.g., the CR2/CD19/CD81 co-activation complex (D. T. Fearon, 1995 ibid.; D. T. Fearon, 1998, ibid.; J. C. Cambier, 1997, ibid; A. K. Matsumoto, et al., J Exp Med 173, 55-64 (1991))). Such cells include B lymphocytes, T lymphocytes, follicular dendritic cells, thymocytes, epithelial cells, and mast cells. Additionally, certain cells may be induced to express CR2, for example, some tumor cells. Therefore, cells that express CR2 can include cells that naturally express CR2, recombinantly express CR2, or which can be induced to express CR2. Cells useful in some embodiments can also include cells that express a natural ligand of CR2, such as CD23.
The assay of the present invention can also be a non-cell based assay. In this embodiment, the candidate compound can be directly contacted with an isolated CR2, or a receptor component (e.g., an isolated extracellular portion of the receptor, or soluble receptor), and the ability of the candidate compound to bind to the receptor or receptor component can be evaluated, such as by an immunoassay or other binding assay. The assay can, if desired, additionally include the step of further analyzing whether candidate compounds which bind to a portion of the receptor are capable of increasing or decreasing the activity of CR2. Such further steps can be performed by cell-based assay, as described above, or by non-cell-based assay. For example, isolated membranes may be used to identify compounds that interact with CR2. Membranes can be harvested from cells expressing CR2 by standard techniques and used in an in vitro binding assay. 125I-labeled (other labels can be used also) ligand (e.g., 125I-labeled C3 d) is contacted with the membranes and assayed for specific activity; specific binding is determined by comparison with binding assays performed in the presence of excess unlabeled ligand. Membranes are typically incubated with labeled ligand in the presence or absence of test compound. Compounds that bind to the receptor and compete with labeled ligand for binding to the membranes reduced the signal compared to the vehicle control samples.
Alternatively, soluble CR2 may be recombinantly expressed and utilized in non-cell based assays to identify compounds that bind to CR2. Recombinantly expressed. CR2 polypeptides or fusion proteins containing one or more extracellular domains of CR2, and preferably, at least SCR1 and SCR2, can be used in the non-cell based screening assays. Alternatively, peptides corresponding to the extracellular domain of CR2 or fusion proteins containing the extracellular domain of CR2 can be used in non-cell based assay systems to identify compounds that bind to the extracellular portion of CR2. In non-cell based assays the recombinantly expressed CR2 is attached to a solid substrate by means well known to those in the art. For example, CR2 and/or cell lysates containing such receptors can be immobilized on a substrate such as: artificial membranes, organic supports, biopolymer supports and inorganic supports. The protein can be immobilized on the solid support by a variety of methods including adsorption, cross-linking (including covalent bonding), and entrapment. Adsorption can be through van del Waal's forces, hydrogen bonding, ionic bonding, or hydrophobic binding. Exemplary solid supports for adsorption immobilization include polymeric adsorbents and ion-exchange resins. Solid supports can be in any suitable form, including in a bead form, plate form, or well form. The test compounds are then assayed for their ability to bind to CR2.
In one embodiment, a BIAcore machine can be used to determine the binding constant of a complex between CR2 and a ligand (e.g., C3d) in the presence and absence of the candidate compound. For example, CR2 or a ligand binding fragment thereof can be immobilized on a substrate. A ligand, such as C3d, is contacted with the substrate to form a CR2-C3d complex. The dissociation constant for the complex can be determined by monitoring changes in the refractive index with respect to time as buffer is passed over the chip (O'Shannessy et al. Anal. Biochem. 212:457-468 (1993); Schuster et al., Nature 365:343-347 (1993)). Contacting a candidate compound at various concentrations with the CR2-ligand complex and monitoring the response function (e.g., the change in the refractive index with respect to time) allows the complex dissociation constant to be determined in the presence of the candidate compound and indicates whether the candidate compound is either an inhibitor or an agonist of the CR2-ligand complex. Alternatively, the candidate compound can be contacted with the immobilized CR2 at the same time as the ligand to see if the candidate compound inhibits or stabilizes the binding of the ligand to CR2.
Other suitable assays for measuring the binding of a candidate compound to a CR2 or CR2 ligand, and or for measuring the ability of such compound to affect the binding of a CR2 to its ligand include, for example, immunoassays such as enzyme linked immunoabsorbent assays (ELISA) and radioimmunoassays (RIA), as well as cell-based assays including, cytokine secretion assays, or intracellular signal transduction assays that determine, for example, protein or lipid phosphorylation, mediator release or intracellular Ca++ mobilization upon CR2 binding to a cell signal transduction molecule or coreceptor.
As used herein, the phrase “agonist” refers to any compound that interacts with a CR2 and elicits an observable response. More particularly, a CR2 agonist can include, but is not limited to, a protein (including an antibody), a peptide, a nucleic acid or any suitable product of drug design (e.g., a mimetic) which is characterized by its ability to agonize (e.g., stimulate, induce, increase, enhance) the biological activity of a naturally occurring CR2 in a manner similar to a natural agonist (e.g., C3d, gp350/220, or CD23) (e.g., by interaction/binding with and/or direct or indirect activation of CR2, including by stabilizing the interaction of CR2 with a natural ligand). An “antagonist” refers to any compound which inhibits the effect of a CR2 agonist, as described above. More particularly, a CR2 antagonist is capable of associating with a CR2 such that the biological activity of the receptor is decreased (e.g., reduced, inhibited, blocked, reversed, altered) in a manner that is antagonistic (e.g., against, a reversal of, contrary to) to the action of a natural agonist on the receptor. It is noted that the three dimensional structures disclosed herein can be used to design or identify candidate compounds that agonize or antagonize the biological activity of the CR2 ligand. For example, a compound that enhances the interaction between CR2 and CD23 can also have a stimulatory effect on a cell that expresses CD23.
Preferred agonists (i.e., stimulatory compounds) to identify using the present method are compounds that exhibit improved binding to CR2 when compared with the ability of a natural CR2 ligand to bind to CR2, and also include compounds that enhance the binding of a natural ligand to CR2 or enhance signal transduction through CR2 coreceptor complexes. Preferred agonists of the present invention are identified by their ability to: (1) bind to, or otherwise interact with, CR2 at a higher level than, for example, a natural CR2 ligand; (2) enhance binding of CR2 to its ligand; (3) enhance dimer formation of CR2 by binding to CR2 or to the combination of CR2 bound to its ligand; and/or (4) enhance signal transduction through CR2. A preferred agonist of the present invention can also include a compound that binds to CR2 or a CR2 ligand, thereby enhancing the binding of CR2 to its ligand or improving cellular signal transduction during or after the binding of CR2 to its ligand, by, for example, modifying other regions of the CR2 by an allosteric interaction that modifies the ligand-binding site of CR2. Another suitable agonist compound of the present invention can include a compound that binds to CR2 in the absence of a natural ligand, in such a manner that CR2-mediated cellular signal transduction is stimulated.
Suitable antagonist (i.e., inhibitory) compounds to identify using the present method are compounds that interact directly with CR2, thereby inhibiting the binding of a natural ligand to CR2, by either blocking the ligand binding site of CR2 (referred to herein as substrate analogs) or by modifying other regions of CR2 (such as in the interface between the monomers of a CR2 dimer, or at the interface between the SCR1 and SCR2 regions of each monomer) such that the natural ligand cannot bind to CR2 (e.g., by allosteric interaction). A CR2 substrate analog refers to a compound that interacts with (e.g., binds to, associates with, modifies) the ligand binding site of a CR2 protein. A CR2 substrate analog can, for example, comprise a chemical compound that mimics the CR2 binding portion of a natural ligand, or that binds specifically to the ligand binding site of CR2 but does not mimic the CR2 binding portion of the natural ligand. An inhibitory compound of the present invention can also include a compound that essentially mimics at least a portion of CR2 that binds to a natural ligand (referred to herein as a peptidomimetic compound). Other suitable inhibitory compounds of the present invention include compounds that inhibit the binding of CR2 to a cell signal inducing molecule such as CD 19.
Various specific embodiments of the present invention are described below. The description of the structure of CR2, and of structure based methods of identifying compounds that regulate CR2 are generally applicable to the methods described below, with particular modifications being noted in the specific description of the methods.
One embodiment of the present invention relates to a method to identify a compound that inhibits the complement receptor type 2 (CR2)-dependent infection of a host cell by Epstein Barr Virus (EBV). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a cell that expresses CR2 or a ligand binding fragment thereof and an Epstein Barr Virus (EBV) particle under conditions in which the EBV particle can bind to CR2 and infect the cell in the absence of the candidate compound; and (d) measuring the intracellular EBV titer of the cell; wherein a candidate inhibitor compound is selected as a compound that inhibits the EBV titer in the cell, as compared to in the absence of the candidate inhibitor compound.
As discussed in the Background section, one of the naturally occurring ligand for human CR2 is Epstein-Barr virus (EBV). EBV interacts with CR2 via the gp350/220 viral membrane protein (J. D. Fingeroth, et al., Proc Natl Acad Sci USA 81, 4510-4 (1984)). EBV causes infectious mononucleosis, and is associated with Burkitt's Lymphoma and several other lymphomas and non-lymphoid tumors (M. Okano, Acta Paediatr 87, 11-8 (1998)). Therefore, the identification of compounds that inhibit the interaction between EBV and CR2 are desirable. Previous studies have suggested that two amino acid positions in CR2 Ser16 and Tyr68 to Tyr, FIG. 2E and green patch in FIGS. 5A and 5B) are likely to be involved in gp350/220 binding (D. R. Martin et al., J Virol 68, 4716-26 (1994)). Given the three dimensional structure of the CR2 disclosed herein, one of skill in the art can now design or identify compounds that are predicted to bind to three dimensional face of CR2 including these residues.
In this embodiment, the steps of providing the CR2 structure and identifying a candidate compound are performed as described above generally for any candidate compound. The step of contacting the candidate can be performed under any suitable conditions for contacting a virus, or portion of the virus (e.g., gp350/220) with a receptor. Such a method preferably includes contacting (e.g., by mixing, adding, combining) EBV with a cell that expresses CR2 or a ligand binding fragment thereof (e.g., naturally, recombinantly or by induction) under conditions wherein, in the absence of the candidate compound, the EBV particle can bind to CR2 and infect the cell. The intracellular viral titer is measured in the presence and in the absence of the compound using methods well known to those of skill in the art. An inhibitor compound is selected as a compound that inhibits the EBV titer in the cell, as compared to in the absence of the candidate inhibitor compound.
Another embodiment of the present invention relates to a method to identify a compound that inhibits the binding of CD23 to complement receptor type 2 (CR2). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a first cell expressing CR2 or a ligand binding fragment thereof and a second cell expressing a CD23 protein or fragment thereof under conditions in which the CD23 protein or fragment thereof and the CR2 or the ligand binding fragment thereof can bind in the absence of the candidate compound; and (d) measuring a biological activity induced by the interaction of CD23 and CR2 in the first or second cell; wherein a candidate inhibitor compound is selected as a compound that inhibits the biological activity as compared to in the absence of the candidate inhibitor compound.
CD23 is a molecule expressed on the follicular dendritic or other cell lineage surfaces which binds to B cells via CR2 (CR2/CD19/CD81 co-activation complex), thereby greatly potentiating signaling via the B cell antigen receptor. The identification of compounds that enhance the binding of CD23 to CR2 would be desirable under conditions when potentiation of the B cell antigen response is desired. However, CD23 is known to enhance IgE isotype switching in B cells. IgE is the prominent immunoglobulin isotype involved in allergic reactions. Inhibition of IgE production would reduce symptoms of allergic inflammation. Therefore, in one embodiment, it is desirable to inhibit the interaction between CR2 and CD23 to reduce IgE isotype switching in B cells.
In this embodiment, the step of contacting the candidate compound identified in step (b) with a first cell expressing CR2 or a ligand binding fragment thereof and a second cell expressing a CD23 protein or fragment thereof occurs under conditions in which the CD23 protein or fragment thereof and the CR2 or the ligand binding fragment thereof can bind in the absence of the candidate compound. Such conditions have been described above for cell-based assays. Preferably, the first cell is a B cell, although any CR2-expressing cell as described herein can be used. The CD23-expressing cell can include a follicular dendritic cell and a cell that recombinantly expresses CD23. Step (d) of measuring a biological activity induced by the interaction of CD23 and CR2 in the first or second cell can include the measurement of any suitable biological activity that is indicative of CR2 activation in the first cell and/or CD23 activation in the second cell. For example, biological activities associated with CR2 activation include, activation of lyn tyrosine kinase, activations of phosphatidyl inositol 3′ kinase, phosphorylation of CD 19, activation of PI3 kinase, and activation of protein kinase C (PKC). If the CR2-expressing cell is a B cell, isotype switching to IgE can be measured, for example, by comparing the amounts of expression of IgE between cells in the presence and absence of the compound. Biological activities associated with CD23 activation include, but are not limited to, increases in IgG synthesis, phosphatidylinositol hydrolysis, cAMP synthesis, Calcium flux, protein tyrosine kinase activation, increases in IL-6 and TNF-a synthesis, nitric oxide activation, increases in CD40 and HLA Class II expression, and NF-KB activation (J. Gordon, Immunol. Today 15, 411-417 (1994); B. Heyman, Ann. Rev. Immunol. 18, 709-737 (2000); V. Fremeaux-Bacchi, et al, Eur. J. Immunol. 28, 4268-4274 (1998); R. M. Ten, et al, J. Immunol. 163, 3851-3857 (1999). Methods of measuring such biological activities of both CR2 and CD23 are known in the art and include immunoassays, kinase assays, flow cytometry, and phosphorylation assays. In this embodiment of the invention, an inhibitor compound is selected as a compound that inhibits the biological activity of CR2 or CD23 as compared to in the absence of the inhibitor compound.
Yet another embodiment of the present invention relates to a method to identify a compound that inhibits the binding of C3d, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, to complement receptor type 2 (CR2). This method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a cell expressing CR2 or a fragment thereof and C3d or a fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, under conditions in which the C3d or fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, can bind to CR2 and enhance cell activation in the absence of the candidate compound; and (d) measuring the activation of the cell; wherein a candidate inhibitor compound is selected as a compound that inhibits cell activation, as compared to in the absence of the candidate inhibitor compound.
C3d-bound antigens, or antigens containing CR2-binding C3 fragments that contain C3d or a portion thereof, amplify B cell responses by binding to CR2 through C3d (or other CR2-binding C3 fragments that contain C3d or a portion thereof) at the same time as engaging the B cell antigen receptor (BCR) via the bound antigen (R. H. Carter and D. T. Fearon, Science 256, 105-7 (1992); J. C. Cambier, Biochem Soc Trans 25, 441-5 (1997)). The cross-linking of CR2 to the BCR by C3d greatly amplifies a signal transduction cascade through the CR2/CD19/CD81 co-activation complex (D. T. Fearon, 1995 ibid.; D. T. Fearon, 1998, ibid.; J. C. Cambier, 1997, ibid.; A. K. Matsumoto, et al., J Exp Med 173, 55-64 (1991)). Therefore, compounds that inhibit this interaction are useful for reducing an immune response and specifically, a humoral immune response (although effects on the cellular immune response may also be achieved). Compounds that enhance or mimic the interaction between CR2 and C3d are useful for potentiating such an immune response.
In this embodiment, the step of contacting the candidate compound identified in step (b) with a cell expressing CR2 or a fragment thereof and C3d or a fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, occurs under conditions in which the C3d or fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, can bind to CR2 and enhance cell activation in the absence of the candidate compound. Such cell-based methods of contacting have been described previously herein. Preferably, the cell expressing CR2 is selected from the group of a B cell, a T cell, a thymocyte, an epithelial cell, and a mast cell. The measurement of cell activation in (d) can be accomplished by any suitable method for detecting CR2 biological activity as previously described herein, and includes, but is not limited to: the measurement of: cytokine production by the cell, calcium mobilization in the cell, lyn tyrosine kinase activity in the cell, phosphatidyl inositol 3′ kinase activity in the cell, phosphorylation of CD19 in the cell, and activation of protein kinase C (PKC) in the cell. An inhibitor compound is selected as a compound that inhibits cell activation, as compared to in the absence of the candidate inhibitor compound.
Another embodiment of the present invention relates to a method to inhibit complement receptor type 2 (CR2)-dependent human immunodeficiency virus-1 (HIV-1) infection of cells in a patient. This method includes the steps of administering to a patient infected with HIV-1 an inhibitor compound that inhibits the binding of C3d, C3 or another CR2-binding fragment of C3 containing C3d or a portion thereof, -opsonized HIV-1 to B cells, follicular dendritic cells, T cells or macrophages in the patient. The inhibitor compound is selected by the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to said CR2 SCR 1-2 region by performing structure based drug design with said structure of (a) to identify a compound structure that binds to said three dimensional structure of said CR2 SCR 1-2 region; (c) contacting said candidate compound identified in step (b) with a B cell, follicular dendritic cell, T cell or macrophage expressing CR2 or a fragment thereof and C3d or a fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, under conditions in which said C3d or fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, can bind to CR2 and enhance activation of the B cell, follicular dendritic cell, T cell or macrophage in the absence of said candidate compound; and (d) measuring the activation of the B cell, follicular dendritic cell, T cell or macrophage, wherein a candidate inhibitor compound is selected as a compound that inhibits activation of the B cell, follicular dendritic cell, T cell or macrophage, as compared to in the absence of said candidate inhibitor compound.
CR2 has been shown to mediate the interaction of C3d-bound HIV-1, or HIV-1 bound to other CR2-binding C3 fragments that contain C3d or a portion thereof, as an immune complex with B cells in a fashion that promotes transfer of virus and infection of CD4 T cells (S. Moir, et al., J Exp Med 192, 637-46 (2000)). Therefore, it would be desirable to design or identify compounds that inhibit the interaction of C3d, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof with CR2 on B cells, follicular dendritic cells, T cells and macrophages to reduce the infection of CD4 T cells by HIV-1. In this embodiment, the step of contacting the candidate compound identified in step (b) with a B cell, follicular dendritic cell, T cell or macrophage, expressing CR2 or a fragment thereof and C3d or a fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, occurs under conditions in which the C3d or fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, can bind to CR2 and enhance activation of the B cell, follicular dendritic cell, T cell or macrophage in the absence of the candidate compound. Such conditions have been described in detail above. In addition, the step of measuring the activation of the B cell, follicular dendritic cell, T cell or macrophage expressing CR2 (i.e., by measuring a biological activity effected by CR2) have been described above.
Once a compound has been identified that inhibits the interaction between C3d and CR2 on B cells, follicular dendritic cells, T cells and/or macrophages, the compound is administered to a patient infected with HIV-1. A preferred patient to treat includes a patient with early-onset HIV infection. Such a patient can be defined herein as a patient that meets one or more of the following criteria: (1) the patient has a blood CD4+ T cell count of at least about 100 cells/mm3, and preferably, at least about 200 cells/mm3, and more preferably, at least about 300 cells/mm3, and even more preferably, at least about 400 cells/mm3 as determined within 30 days of the time of employment of the present method; and (2) the patient has an HIV serum load of less than about 400 copies/ml, and preferably, less than to about 300 copies/ml, and more preferably, less than about 200 copies/ml, and even more preferably, less than about 100 copies/ml, and most preferably undetectable viral load, as determined by plasma RNA PCT within 30 days of when the method is employed. In one embodiment, the patient is characterized as having a CD4+ T cell count of at least about 100 cells/mm3 when the method is employed and/or an HIV viral load of less than about 400 copies/ml when the method is employed.
A composition to be administered to a patient, such as in this embodiment, generally includes the compound identified by the structure based identification method and a carrier, and preferably, a pharmaceutically acceptable carrier. According to the present invention, a “pharmaceutically acceptable carrier” includes pharmaceutically acceptable excipients and/or pharmaceutically acceptable delivery vehicles, which are suitable for use in administration of the composition to a suitable in vitro, ex vivo or in vivo site. A suitable in vitro, in vivo or ex vivo site is preferably at or near a cell that expresses a CR2, and most preferably, at or near a site of interest in the patient. Preferred pharmaceutically acceptable carriers are capable of maintaining a compound identified by the present methods in a form that, upon arrival of compound at the cell target in a culture or in patient, the compound is capable of interacting with its target (e.g., a CR2).
Suitable excipients of the present invention include excipients or formularies that transport or help transport, but do not specifically target a composition to a cell (also referred to herein as non-targeting carriers). Examples of pharmaceutically acceptable excipients include, but are not limited to water, phosphate buffered saline, Ringer's solution, dextrose solution, serum-containing solutions, Hank's solution, other aqueous physiologically balanced solutions, oils, esters and glycols. Aqueous carriers can contain suitable auxiliary substances required to approximate the physiological conditions of the recipient, for example, by enhancing chemical stability and isotonicity.
Suitable auxiliary substances include, for example, sodium acetate, sodium chloride, sodium lactate, potassium chloride, calcium chloride, and other substances used to produce phosphate buffer, Tris buffer, and bicarbonate buffer. Auxiliary substances can also include preservatives, such as thimerosal, —or o-cresol, formalin and benzol alcohol. Compositions of the present invention can be sterilized by conventional methods and/or lyophilized.
One type of pharmaceutically acceptable carrier includes a controlled release formulation that is capable of slowly releasing a composition of the present invention into a patient or culture. As used herein, a controlled release formulation comprises a compound of the present invention (e.g., a protein (including homologues), a drug, an antibody, a nucleic acid molecule, or a mimetic) in a controlled release vehicle. Suitable controlled release vehicles include, but are not limited to, biocompatible polymers, other polymeric matrices, capsules, microcapsules, microparticles, bolus preparations, osmotic pumps, diffusion devices, liposomes, lipospheres, and transdermal delivery systems. Other carriers of the present invention include liquids that, upon administration to a patient, form a solid or a gel in situ. Preferred carriers are also biodegradable (i.e., bioerodible). When the compound is a recombinant nucleic acid molecule, suitable delivery vehicles include, but are not limited to liposomes, viral vectors or other delivery vehicles, including ribozymes. Natural lipid-containing delivery vehicles include cells and cellular membranes. Artificial lipid-containing delivery vehicles include liposomes and micelles. A delivery vehicle of the present invention can be modified to target to a particular site in a patient, thereby targeting and making use of a compound of the present invention at that site. Suitable modifications include manipulating the chemical formula of the lipid portion of the delivery vehicle and/or introducing into the vehicle a targeting agent capable of specifically targeting a delivery vehicle to a preferred site, for example, a preferred cell type. Other suitable delivery vehicles include gold particles, poly-L-lysine/DNA-molecular conjugates, and artificial chromosomes.
A pharmaceutically acceptable carrier which is capable of targeting is herein referred to as a “delivery vehicle.” Delivery vehicles of the present invention are capable of delivering a composition of the present invention to a target site in a patient. A “target site” refers to a site in a patient to which one desires to deliver a composition. For example, a target site can be any cell which is targeted by direct injection or delivery using liposomes, viral vectors or other delivery vehicles, including ribozymes and antibodies. Examples of delivery vehicles include, but are not limited to, artificial and natural lipid-containing delivery vehicles, viral vectors, and ribozymes. Natural lipid-containing delivery vehicles include cells and cellular membranes. Artificial lipid-containing delivery vehicles include liposomes and micelles. A delivery vehicle of the present invention can be modified to target to a particular site in a subject, thereby targeting and making use of a compound of the present invention at that site. Suitable modifications include manipulating the chemical formula of the lipid portion of the delivery vehicle and/or introducing into the vehicle a compound capable of specifically targeting a delivery vehicle to a preferred site, for example, a preferred cell type. Specifically, targeting refers to causing a delivery vehicle to bind to a particular cell by the interaction of the compound in the vehicle to a molecule on the surface of the cell. Suitable targeting compounds include ligands capable of selectively (i.e., specifically) binding another molecule at a particular site. Examples of such ligands include antibodies, antigens, receptors and receptor ligands. Manipulating the chemical formula of the lipid portion of the delivery vehicle can modulate the extracellular or intracellular targeting of the delivery vehicle. For example, a chemical can be added to the lipid formula of a liposome that alters the charge of the lipid bilayer of the liposome so that the liposome fuses with particular cells having particular charge characteristics. In one embodiment, a targeting carrier can be a portion of a CR2 protein as described elsewhere herein, which is linked to the compound.
One preferred delivery vehicle of the present invention is a liposome. A liposome is capable of remaining stable in an animal for a sufficient amount of time to deliver a nucleic acid molecule or other compound to a preferred site in the animal. A liposome, according to the present invention, comprises a lipid composition that is capable of delivering a nucleic acid molecule or other compound to a particular, or selected, site in a patient. A liposome according to the present invention comprises a lipid composition that is capable of fusing with the plasma membrane of the targeted cell to deliver a nucleic acid molecule or other compound into a cell. Suitable liposomes for use with the present invention include any liposome. Preferred liposomes of the present invention include those liposomes commonly used in, for example, gene delivery methods known to those of skill in the art. More preferred liposomes comprise liposomes having a polycationic lipid composition and/or liposomes having a cholesterol backbone conjugated to polyethylene glycol. Complexing a liposome with a nucleic acid molecule or other compound can be achieved using methods standard in the art.
A liposome delivery vehicle is preferably capable of remaining stable in a patient for a sufficient amount of time to deliver a nucleic acid molecule or other compound of the present invention to a preferred site in the patient (i.e., a target cell). A liposome delivery vehicle of the present invention is preferably stable in the patient into which it has been administered for at least about 30 minutes, more preferably for at least about 1 hour and even more preferably for at least about 24 hours. A preferred liposome delivery vehicle of the present invention is from about 0.01 microns to about 1 microns in size.
Another preferred delivery vehicle comprises a viral vector. A viral vector includes an isolated nucleic acid molecule useful in the present invention, in which the nucleic acid molecules are packaged in a viral coat that allows entrance of DNA into a cell. A number of viral vectors can be used, including, but not limited to, those based on alphaviruses, poxviruses, adenoviruses, herpesviruses, lentiviruses, adeno-associated viruses and retroviruses.
A composition which includes an compound identified according to the present methods can be delivered to a cell culture or patient by any suitable method. Selection of such a method will vary with the type of compound being administered or delivered (i.e., protein, peptide, nucleic acid molecule, mimetic, or other type of compound), the mode of delivery (i.e., in vitro, in vivo, ex vivo) and the goal to be achieved by administration/delivery of the compound or composition. According to the present invention, an effective administration protocol (i.e., administering a composition in an effective manner) comprises suitable dose parameters and modes of administration that result in delivery of a composition to a desired site (i.e., to a desired cell) and/or in the desired regulatory event (e.g., inhibition of the binding of C3d-opsonized HIV-1 to B cells or follicular dendritic cells in the patient).
Administration routes include in vivo, in vitro and ex vivo routes. In vivo routes include, but are not limited to, oral, nasal, intratracheal injection, inhaled, transdermal, rectal, and parenteral routes. Preferred parenteral routes can include, but are not limited to, subcutaneous, intradermal, intravenous, intramuscular and intraperitoneal routes. Intravenous, intraperitoneal, intradermal, subcutaneous and intramuscular administrations can be performed using methods standard in the art. Aerosol (inhalation) delivery can also be performed using methods standard in the art (see, for example, Stribling et al., Proc. Natl. Acad. Sci. USA 189:11277-11281, 1992, which is incorporated herein by reference in its entirety). Oral delivery can be performed by complexing a therapeutic composition of the present invention to a carrier capable of withstanding degradation by digestive enzymes in the gut of an animal. Examples of such carriers, include plastic capsules or tablets, such as those known in the art. Direct injection techniques are particularly useful for suppressing graft rejection by, for example, injecting the composition into the transplanted tissue, or for site-specific administration of a compound, such as at the site of a tumor. Ex vivo refers to performing part of the regulatory step outside of the patient, such as by transfecting a population of cells removed from a patient with a recombinant molecule comprising a nucleic acid sequence encoding a protein according to the present invention under conditions such that the recombinant molecule is subsequently expressed by the transfected cell, and returning the transfected cells to the patient. In vitro and ex vivo routes of administration of a composition to a culture of host cells can be accomplished by a method including, but not limited to, transfection, transformation, electroporation, microinjection, lipofection, adsorption, protoplast fusion, use of protein carrying agents, use of ion carrying agents, use of detergents for cell permeabilization, and simply mixing (e.g., combining) a compound in culture with a target cell.
In this particular embodiment of the invention (i.e., the inhibition of HIV infection), it will be obvious to one of skill in the art that the number of doses administered to an immunodeficiency virus infected patient is dependent upon the extent of the infection and the response of an individual to the treatment. For example, in the case of HIV-infection, a patient having a high titer of HIV may require more doses than a patient having lower titers. In some cases, however, a patient having a high titer of HIV may require fewer doses than a patient having lower titers, if the patient with the high titer responds more favorably to the therapeutic composition than the patient with the lower titer. Thus, it is within the scope of the present invention that a suitable number of doses, as well as the time periods between administration, includes any number required to cause regression of a disease.
In another embodiment, this method is employed in conjunction with administration to the patient of one or more anti-retroviral therapeutic compounds. Such compounds include, but are not limited to, AZT, ddI, ddC, d4T, 3TC and/or protease inhibitors.
Another embodiment of the present invention relates to a method of preparing a vaccine, comprising linking a compound that increases B cell activation to an antigen to form the vaccine. The compound is selected by a method including the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) contacting the candidate compound identified in step (b) with a B cell expressing CR2 or a fragment thereof and with C3d or a fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, under conditions in which said C3d or fragment thereof, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof, can bind to and activate CR2 in the absence of said candidate compound; and (d) measuring the activation of the B cell. A candidate compound for use in a vaccine is selected as a compound that increases B cell activation as compared to in the absence of the candidate compound.
Because CR2 plays a critical role as a coreceptor for B cells and is expressed on other cells as well, CR2 is a molecular target for adjuvants and can enhance the immune response to vaccines. Therefore, in this method, compounds are identified that bind to CR2 and that enhance B cell activation, either by enhancing the interaction between CR2 and a natural ligand (e.g., C3d), or by directly interacting with CR2 to enhance downstream biological activities of the receptor, as previously discussed herein. Methods for contacting a cell with the compound and measuring the activation events associated with CR2 activation have been previously described. In addition, to measure B cell activation, one can measure calcium mobilization, immunoglobulin class switching, cytokine production, activation of NF-KB, activation of MAP kinases, protein kinase activity and phosphorylation of proteins associated with B cell activation. In this embodiment, the conditions under which the B cell is contacted typically include the presence of an antigen that binds to the B cell antigen receptor, in addition to the other components. A compound for use in a vaccine, once identified, is typically associated with a protein (antigen or antigen-containing composition) or nucleic acid to be administered to a patient as part of the vaccine. The use of a compound identified by the present method will potentiate the immune response to the antigen.
Another embodiment of the present invention relates to a drug delivery system that will preferentially deliver compounds to sites of complement activation containing CR2-binding fragments of C3 (e.g., C3 and portions thereof that contain C3d). Such a drug delivery system includes: (a) a drug; and, (b) a portion of a CR2 protein that includes one or more of: (i) a portion comprising positions on strand B and the B-C loop of SCR2 including: G79-G80-Y81-K82-I83-R84-G85-S86-T87-P88-Y89; (ii) a portion comprising position K100 on the B strand of CR2; and, (iii) a portion comprising positions: V130-F131-P132-L133 (positions given with reference to SEQ ID NO:4). In one embodiment, the portion of the CR2 protein can also contain positions T101-N102-F103 (reference again to SEQ ID NO:4). The drug is linked to the portion of CR2 by any suitable method, covalently or non-covalently, including by recombinant means or by chemical means. In this embodiment, the CR2 is not a full-length protein, or the soluble form of CR2, as it is known in the art (i.e., the natural soluble CR2 or the CR2 with the membrane portion removed), but rather, includes less of the amino acid sequence than the full-length or the soluble CR2, and preferably, just the portions of SCR1 and SCR2 that have been determined herein to be involved in the contact between CR2 and a natural ligand (e.g., C3d) and that are required to form a CR2 portion with the tertiary structure necessary to bind to C3d (or a fragment thereof). Therefore, the portion of CR2 used in the drug delivery system consists essentially of at least one or more of the above-recited segments of CR2; including a contiguous segment containing all of the segments (i.e., from positions 79-133 of SEQ ID NO:4), and has the three dimensional conformation of CR2 at the CR2-C3d interface, such that the portion will bind to C3d, C3 or other CR2-binding fragments of C3 that contain C3d or a portion thereof. Therefore, the portion of CR2 suitable for use in a drug delivery system includes the portions of CR2 that contact C3d, as well as the portions required to maintain the spatial positions of the contact residues, such that the tertiary structure of the C3d binding portion is conformationally similar to the tertiary structure of the C3d binding portion of the CR2 crystal described herein, using the parameters for structural homologues as described elsewhere herein for the structure of the CR2 complexed with C3d. According to the present invention, a CR2 fragment consisting essentially of the portions of SCR1 and SCR2 that have been determined herein to be involved in the contact between CR2 and C3d can have at least one, and up to about 20 (in whole number increments), additional heterologous amino acids flanking each of the C- and/or N-terminal end of the CR2 portion that contains the above-described segments and the sequence necessary to maintain the appropriate tertiary structure to bind to C3d (or a fragment thereof). According to the present invention, the heterologous amino acids are a sequence of amino acids that are not naturally found (i.e., not found in nature, in vivo) flanking the CR2 sequence that makes up the portion of CR2 or which would not be encoded by the nucleotides that flank the naturally occurring CR2 nucleic acid sequence as it occurs in the gene, if such nucleotides in the naturally occurring sequence were translated using standard codon usage for the organism from which the given CR2 portion is derived. Such heterologous amino acids can include a sequence that is less than about 75% similar to the natural sequence in the same positions. This embodiment also includes methods of identifying such portions of CR2.
Drugs that are desirable to deliver using the drug delivery system of the present invention include any drug that may have a beneficial effect on a subject when delivered to a site of complement activation wherein C3 and/or CR2-binding portions of C3 are present. The drugs can be protein-based, carbohydrate-based, lipid-based, nucleic acid-based, or any small molecule. Examples of such drugs include, but are not limited to, anti-inflammatory compounds, cytotoxic drugs, complement regulatory proteins, corticosteroids, and any compounds useful in ischemic, inflammatory autoimmune or vascular diseases, all of which have C3 fragments present.
In this embodiment, drug design strategies as specifically described above with regard to the identification of compounds that bind to CR2 and affect its interaction with various ligands can be similarly applied to the CR2 structure itself. CR2 proteins designed by this method can be used as drug delivery vehicles or to otherwise alter the biological activity of a CR2, such as by competing for a naturally occurring CR2 in vivo. One of ordinary skill in the art, using the art recognized modeling programs and drug design methods, many of which are described herein, to prepare portions of complement receptor type 2 (CR2) proteins that bind to their ligands, including CR2 homologues that retain ligand binding activity. In addition, one of skill in the art can produce CR2 proteins having modified biological activity. For example, such a method can include: (a) providing a three dimensional structure of a CR2 SCR1-2 domain as previously described herein; (b) analyzing the three dimensional structure to the three-dimensional structure of the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to the sites in the structure contributing to ability of CR2 to bind to a ligand (e.g., C3d or other CR2-binding fragments of C3); and (c) producing a protein that is a portion of CR2 that includes such sites. In the method to produce a CR2 protein having modified biological activity, one can analyze the three dimensional structure of CR2 provided herein to identify at least one site that contributes to the biological activity of the protein, and then modify at least one such site to alter the biological activity of the CR2 protein. Methods to altered proteins for CR2 biological activity include testing the altered protein for any of the biological activities of CR2 previously described herein.
Another embodiment of the present invention relates to an antibody that selectively binds to CR2. The antibody binds to a portion of CR2 selected from the group consisting of: (a) the interface between the SCR1 and SCR2 domains of CR2; (b) the dimer interface between two CR2 proteins; and, (c) the interface between CR2 and C3d (where the C3d includes any CR2-binding fragments of C3 that contain C3d or a portion thereof). The portion of the CR2-C3d interface bound by the antibody preferably includes at site selected from: (a) the B strand and the B-C loop of CR2 SCR2 comprising the segment: G79-G80-Y81-K82-183-R84-G85-S86-T87-P88-Y89; (b) the B strand of CR2 SCR2 comprising position K100; and (c) a segment of CR2 SCR2 comprising V130-F131-P132-L133. Prior to the present invention, the three dimensional structure of the CR2 interfaces set forth above were not known and therefore, it was not possible to design or identify an antibody by making use of such structural information. The present inventors have provided suitable target sites, including specific residues within such sites, for the design and identification of antibodies.
According to the present invention, the phrase “selectively binds to” refers to the ability of an antibody, antigen binding fragment or binding partner of the present invention to preferentially bind to specified proteins (e.g., the recited portions of a CR2 of the present invention). More specifically, the phrase “selectively binds” refers to the specific binding of one protein to another (e.g., an antibody, fragment thereof, or binding partner to an antigen), wherein the level of binding, as measured by any standard assay (e.g., an immunoassay), is statistically significantly higher than the background control for the assay. For example, when performing an immunoassay, controls typically include a reaction well/tube that contain antibody or antigen binding fragment alone (i.e., in the absence of antigen), wherein an amount of reactivity (e.g., non-specific binding to the well) by the antibody or antigen binding fragment thereof in the absence of the antigen is considered to be background. Binding can be measured using a variety of methods standard in the art including enzyme immunoassays (e.g., ELISA), immunoblot assays, etc. Limited digestion of an immunoglobulin with a protease may produce two fragments.
An antigen binding fragment is referred to as an Fab, an Fab′, or an F(ab′)2 fragment. A fragment lacking the ability to bind to antigen is referred to as an Fc fragment. An Fab fragment comprises one arm of an immunoglobulin molecule containing a L chain (VL+CL domains) paired with the VH region and a portion of the CH region (CH1domain). An Fab′ fragment corresponds to an Fab fragment with part of the hinge region attached to the CH1 domain. An F(ab′)2 fragment corresponds to two Fab′ fragments that are normally covalently linked to each other through a di-sulfide bond, typically in the hinge regions.
Functional aspects of an immunoglobulin molecule include the valency of an immunoglobulin molecule, the affinity of an immunoglobulin molecule, and the avidity of an immunoglobulin molecule. As used herein, affinity refers to the strength with which an immunoglobulin molecule binds to an antigen at a single site on an immunoglobulin molecule (i.e., a monovalent Fab fragment binding to a monovalent antigen). Affinity differs from avidity which refers to the sum total of the strength with which an immunoglobulin binds to an antigen. Immunoglobulin binding affinity can be measured using techniques standard in the art, such as competitive binding techniques, equilibrium dialysis or BIAcore methods. As used herein, valency refers to the number of different antigen binding sites per immunoglobulin molecule (i.e., the number of antigen binding sites per antibody molecule of antigen binding fragment). For example, a monovalent immunoglobulin molecule can only bind to one antigen at one time, whereas a bivalent immunoglobulin molecule can bind to two or more antigens at one time, and so forth. Both monovalent and bivalent antibodies that selectively bind to CR2 of the present invention are encompassed herein.
In one embodiment of the present invention, a monovalent antibody can be used as a regulatory compound. Such an antibody is not capable of aggregating receptors. Divalent antibodies can also be used in the present invention.
In one embodiment, the antibody is a bi- or multi-specific antibody. A bi-specific (or multi-specific) antibody is capable of binding two (or more) antigens, as with a divalent (or multivalent) antibody, but in this case, the antigens are different antigens (i.e., the antibody exhibits dual or greater specificity). A bi-specific antibody suitable for use in the present method includes an antibody having: (a) a first portion (e.g., a first antigen binding portion) which binds to CR2; and (b) a second portion which binds to a cell surface molecule expressed by a cell which expresses CR2. In this embodiment, the second portion can bind to any cell surface molecule. In a preferred embodiment, the second portion is capable of targeting the regulatory antibody to a specific target cell (i.e., the regulatory antibody binds to a target molecule).
Isolated antibodies of the present invention can include serum containing such antibodies, or antibodies that have been purified to varying degrees. Whole antibodies of the present invention can be polyclonal or monoclonal. Alternatively, functional equivalents of whole antibodies, such as antigen binding fragments in which one or more antibody domains are truncated or absent (e.g., Fv, Fab, Fab′, or F(ab)2 fragments), as well as genetically-engineered antibodies or antigen binding fragments thereof, including single chain antibodies or antibodies that can bind to more than one epitope (e.g., bi-specific antibodies), or antibodies that can bind to one or more different antigens (e.g., bi- or multi-specific antibodies), may also be employed in the invention.
Genetically engineered antibodies of the invention include those produced by standard recombinant DNA techniques involving the manipulation and re-expression of DNA encoding antibody variable and/or constant regions. Particular examples include, chimeric antibodies, where the VH and/or VL domains of the antibody come from a different source to the remainder of the antibody, and CDR grafted antibodies (and antigen binding fragments thereof), in which at least one CDR sequence and optionally at least one variable region framework amino acid is (are) derived from one source and the remaining portions of the variable and the constant regions (as appropriate) are derived from a different source. Construction of chimeric and CDR-grafted antibodies are described, for example, in European Patent Applications: EP-A 0194276, EP-A 0239400, EP-A 0451216 and EP-A 0460617.
Alternative methods, employing, for example, phage display technology (see for example U.S. Pat. Nos. 5,969,108, 5,565,332, 5,871,907, 5,858,657) or the selected lymphocyte antibody method of U.S. Pat. No. 5,627,052 may also be used for the production of antibodies and/or antigen fragments of the invention, as will be readily apparent to the skilled individual.
Generally, in the production of an antibody, a suitable experimental animal, such as, for example, but not limited to, a rabbit, a sheep, a hamster, a guinea pig, a mouse, a rat, or a chicken, is exposed to an antigen against which an antibody is desired. Typically, an animal is immunized with an effective amount of antigen that is injected into the animal. An effective amount of antigen refers to an amount needed to induce antibody production by the animal. The animal's immune system is then allowed to respond over a pre-determined period of time. The immunization process can be repeated until the immune system is found to be producing antibodies to the antigen. In order to obtain polyclonal antibodies specific for the antigen, serum is collected from the animal that contains the desired antibodies (or in the case of a chicken, antibody can be collected from the eggs). Such serum is useful as a reagent. Polyclonal antibodies can be further purified from the serum (or eggs) by, for example, treating the serum with ammonium sulfate.
Monoclonal antibodies may be produced according to the methodology of Kohler and Milstein (Nature 256:495-497, 1975). For example, B lymphocytes are recovered from the spleen (or any suitable tissue) of an immunized animal and then fused with myeloma cells to obtain a population of hybridoma cells capable of continual growth in suitable culture medium. Hybridomas producing the desired antibody are selected by testing the ability of the antibody produced by the hybridoma to bind to the desired antigen.
Another embodiment of the present invention relates to a therapeutic composition that, when administered to an animal, enhances B cell responses in the animal. The therapeutic composition includes a compound that stimulates the activity of a complement receptor type 2 (CR2), which has been identified by a method of structure based identification of compounds of the present invention, as described in detail above. Specifically, this method includes the steps of: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as described previously herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) synthesizing the candidate compound; and (d) selecting candidate compounds that bind to and activate CR2.
Another embodiment of the present invention relates to a therapeutic composition that, when administered to an animal, inhibits the biological activity of complement receptor type 2 (CR2) in the animal. The therapeutic composition comprises a compound that inhibits the activity of a complement receptor type 2 (CR2), the compound being identified by the method comprising: (a) providing a three dimensional structure of a CR2 short consensus repeat (SCR) 1-2 region as previously described herein; (b) identifying a candidate compound for binding to the CR2 SCR 1-2 region by performing structure based drug design with the structure of (a) to identify a compound structure that binds to the three dimensional structure of the CR2 SCR 1-2 region; (c) synthesizing the candidate compound; and (d) selecting candidate compounds that inhibit the biological activity of CR2. Preferably, the compounds inhibit the formation of a complex between CR2 and a CR2 ligand, such ligand including, but not limited to, C3d, CD23 and Epstein Barr Virus (EBV). In a more preferred embodiment, the compound inhibits the activation of CR2.
Methods of identifying candidate compounds and selecting compounds that bind to and activate or inhibit CR2 have been previously described herein. Candidate compounds can be synthesized using techniques known in the art, and depending on the type of compound. Synthesis techniques for the production of non-protein compounds, including organic and inorganic compounds are well known in the art.
For smaller peptides, chemical synthesis methods are preferred. For example, such methods include well known chemical procedures, such as solution or solid-phase peptide synthesis, or semi-synthesis in solution beginning with protein fragments coupled through conventional solution methods. Such methods are well known in the art and may be found in general texts and articles in the area such as: Merrifield, 1997, Methods Enzymol. 289:3-13; Wade et al., 1993, Australas Biotechnol. 3(6):332-336; Wong et al., 1991, Experientia 47(11-12):1123-1129; Carey et al., 1991, Ciba Found Symp. 158:187-203; Plaue et al., 1990, Biologicals 18(3):147-157; Bodanszky, 1985, Int. J. Pept. Protein Res. 25(5):449-474; or H. Dugas and C. Penney, BIOORGANIC CHEMISTRY, (1981) at pages 54-92, all of which are incorporated herein by reference in their entirety. For example, peptides may be synthesized by solid-phase methodology utilizing a commercially available peptide synthesizer and synthesis cycles supplied by the manufacturer. One skilled in the art recognizes that the solid phase synthesis could also be accomplished using the FMOC strategy and a TFA/scavenger cleavage mixture.
If larger quantities of a protein are desired, or if the protein is a larger polypeptide, the protein can be produced using recombinant DNA technology. A protein can be produced recombinantly by culturing a cell capable of expressing the protein (i.e., by expressing a recombinant nucleic acid molecule encoding the protein) under conditions effective to produce the protein, and recovering the protein. Effective culture conditions include, but are not limited to, effective media, bioreactor, temperature, pH and oxygen conditions that permit protein production. An effective medium refers to any medium in which a cell is cultured to produce the protein. Such medium typically comprises an aqueous medium having assimilable carbon, nitrogen and phosphate sources, and appropriate salts, minerals, metals and other nutrients, such as vitamins. Recombinant cells (i.e., cells expressing a nucleic acid molecule encoding the desired protein) can be cultured in conventional fermentation bioreactors, shake flasks, test tubes, microtiter dishes, and petri plates. Culturing can be carried out at a temperature, pH and oxygen content appropriate for a recombinant cell. Such culturing conditions are within the expertise of one of ordinary skill in the art. Such techniques are well known in the art and are described, for example, in Sambrook et al., 1988, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. or Current Protocols in Molecular Biology (1989) and supplements.
As discussed above, a composition, and particularly a therapeutic composition, of the present invention generally includes the therapeutic compound (e.g., the compound identified by the structure based identification method) and a carrier, and preferably, a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers and preferred methods of administration of therapeutic compositions of the present invention have been described in detail above with regard to the administration of an inhibitor compound to a patient infected with HIV. Such carriers and administration protocols are applicable to this embodiment.
Yet another embodiment of the present invention relates to an isolated C3d mutant protein, C3 or other CR2-binding fragments of C3 that contain a mutant C3d or a portion thereof, comprising an amino acid substitution of an non-asparagine amino acid residue at position 170 wherein said C3d mutant protein, C3 or other CR2-binding fragments of C3 that contain the mutant C3d or a portion thereof, has reduced binding to complement receptor type 2 (CR2), as compared to a wild-type C3d protein (SEQ ID NO:7), or equivalent wild-type CR2-binding fragment of C3 that contain C3d or a portion thereof. Preferably, the mutant protein is at least about 50% identical to SEQ ID NO:7, and more preferably at least about 60% identical, and more preferably at least about 70% identical, and more preferably at least about 80% identical, and more preferably at least about 90% identical, and more preferably at least about 95% identical to SEQ ID NO:7. In a preferred embodiment, the mutant protein comprises SEQ ID NO:8 (mt170 or mut2) or SEQ ID NO:9 (mut4).
Another embodiment of the present invention relates to a computer for producing a three-dimensional model of a molecule or molecular structure, wherein the molecule or molecular structure comprises a three dimensional structure defined by atomic coordinates of a complement receptor type 2 (CR2) protein, according to Table 2 or Table 3, or a three-dimensional model of a homologue of the molecule or molecular structure, wherein the homologue comprises a three dimensional structure that has an average root-mean-square deviation (RMSD) of equal to or less than about 1.0 Å for the backbone atoms in secondary structure elements in the CR2 protein, wherein the computer comprises:

    
    
        a) a computer-readable medium encoded with the atomic coordinates of the CR2 protein, according to Table 2 or Table 3, to create an electronic file;
        b) a working memory for storing a graphical display software program for processing the electronic file;
        c) a processor coupled to the working memory and to the computer-readable medium which is capable of representing the electronic file as the three dimensional model; and,
        d) a display coupled to the processor for visualizing the three dimensional model;
    
    


wherein the three dimensional structure of the CR2 protein is displayed on the computer.
The following examples are provided for the purpose of illustration and are not to intended to limit the scope of the present invention.
EXAMPLES
Example 1
The following example describes the crystallization and structure determination of the complex of complement receptor type 2 (CR2) and C3d.
Crystallization and Structure Determination of Structure
The crystals of the complex of CR2-C3d were obtained by co-crystallization of CR2 and C3d, at a protein ratio where no free CR2 or C3d could be detected by native gel electrophoresis. The protein concentration of 20 mg/ml was used for crystallization by the method of hanging drop vapor diffusion. The crystallization buffer contained 17% PEG 2K, 0.2 M ZnAcetate, and 0.1 M NaCacodylate (pH 7.36). Crystals reached full size after 4-6 weeks at 4° C. Crystal was frozen under liquid nitrogen in the crystallization buffer containing 20% glycerol. Synchrotron data were collected at Brookhaven National Laboratory and was indexed, integrated and reduced using D*trek (licensed through MSC Inc., Table 1). The space group is R32, with unit cell a=b=170.5 Å, c=173.8 Å. AmoRe (CCP4, Acta Cryst. D50, 760-763 (1994)) was used to do molecular replacement that was carried out using C3d (Accession No. 1C3D from the Protein Data Bank (PDB)) as a search model. The final correlation function and R factor after rotation and translation search were 50% and 45%. Initial phase improvement was carried out using solvent flattening and two fold averaging by the program DM (CCP4, Acta Cryst. D50, 760-763 (1994)) in CCP4 suit. Stepwise model building and refinement were carried out using program “O” and CNS (P. D. A. A. T. Brunger, G. M. Clore, W. L. Delano, P. Gros, R. W. Grosse-Kunstleve, J.-S. Jiang, J. Kuszewski, N. S. P. M. Nilges, R. J. Read, L. M. Rice, T. Simonson, G. L. Warren, Acta Cryst. D54, 905-921 (1998)). The final complete model was refined using simulated annealing, positional refinement, and individual B factor refinement. Water molecules were added last using CNS (Table 1).







TABLE 1





Structure determination and refinement







Data collection statistics










Space group
R32



Unit cell length (Å)
a = b = 170.5, c = 173.8



Resolution (Å)
25.0-2.04



Completeness (last bin)
94.1/83.6



Total reflections
255801



Unique reflections
63919



1Rsym (last bin) %
 6.7/22.3



I/δ (last bin)
10.8/3.7 







Refinement statistics










% of reflections for Rfree
10



Rwork/Rfree
20.8/23.9



rmsd from ideality




Bond length (Å)
0.006



Bond angle (°)
1.10



Dihedral angle (°)
16.8



Ramachandran plot (core,
92.3/0  



disallowed)




Average B factor
33.98



rmsd of B factor (Å2)
1.2



Protein atoms in the model
8878



H2O in the model
580





1Rsym = Σij|(j) − <I(j)>|/Σij Ii (j), where Ii(j) is the i-th measurement of reflection j and <I(j)> is the overall weighted mean of j measurements.






The atomic coordinates representing the structure of the complex of CR2 and C3d were deposited on Jan. 11, 2001, with the Protein Data Bank (PDB), operated by the Research Collaboratory for Structural Bioinformatics (RCSB) (H. M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T. N. Bhat, H. Weissig, I. N. Shindyalov, P. E. Boume, The Protein Data Bank; Nucleic Acids Research, 28:235-242 (2000)), under PDB Deposit No. PDB id 1GHQ. These atomic coordinates are also represented herein as Table 2.
Description of the Overall Structure of the CR2-C3d Complex
FIGS. 1A and 1B show the structure of the CR2-C3d complex. FIG. 1A is an overall view of the structure of CR2 binding to C3d, showing only SCR2 contacting one portion of the edge of C3d. CR2 SCR1 is colored in red and SCR2 in yellow, while C3d is in cyan. The side chain of residue Q20 of C3d that forms an ester-bond with antigen is drawn in pink and labeled. The position of residue D223 of C3d, which is the C3F form (fast migrating variant on agarose gel electrophoresis) that is associated with an increased incidence of certain diseases (M. C. Poznansky et al., J Immunol 143, 1254-8 (1989)), is also labeled. The other form of C3d, C3 S (slow migration), has an N223 residue. The N and C termini of C3d (N1 and C307, respectively) are positioned next to each other. FIG. 1B shows the overall structure showing a second CR2-C3d complex (colored in light blue and grey) that dimerizes with the first one in FIG. 1A. The dimerization contact in the two-complex structure is through SCR1 of CR2 at the very top. (Prepared with the program MOLSCRIPT).
The complex contains a V-shaped CR2 receptor binding to a globular C3d ligand (FIG. 1A). The CR2 receptor portion contains two domains (SCR1 and SCR2) that pack against each other almost side to side, producing a drastic bend of 53 degrees between the two domains to give a V-shape. A well-structured linker of 8 amino acids (residues 64-71) connects the SCR1 and SCR2 domains. Both SCR1 and SCR2 domains consist of only beta-strands and coils, which is characteristic of the SCR fold that contains a beta-barrel core structure (A. P. Wiles, et al., J Mol Biol 272, 253-65 (1997); P. N. Barlow, et al., J Mol Biol 232, 268-84 (1993); J. M. Casasnovas, M. Larvie, T. Stehle, EMBO J18, 2911-22 (1999); R. Schwarzenbacher, et al., EMBO J. 18, 6228-39 (1999)). The V-shaped two-domain CR2 molecule has a span of 42.6 Å at the base of the molecule (from SCR1 to SCR2), and the height of “V” structure (from base to tip) measures 38.5 Å.
The C3d ligand, which has a dome-shaped structure that consists of mostly alpha helices (B. Nagar, R. G. Jones, R. J. Diefenbach, D. E. Isenman, J. M. Rini, Science 280, 1277-81 (1998)), interacts with the receptor using one portion of the edge of the dome. The CR2-contact edge of C3d is located on nearly the opposite side of the amino (N) and carboxyl (C) termini, which are physically proximate to each other (FIG. 1A). Binding to CR2 through this edge leaves the concave and convex surfaces free and the rest of the iC3b molecule (a form of C3 encompassing the C3d domain with which CR2 also interacts) likely oriented away from CR2 (FIG. 1A). The site of the ester-bond linkage to antigens (Q20) sits approximately half way between the receptor contact edge and the N and C termini of C3d (FIG. 1A). The site of mutation (N223 to D223, FIG. 1A) that defines an important disease-related allelic polymorphism, C3F (fast) versus C3S (slow) (M. C. Poznansky, P. M. Clissold, P. J. Lachmann, J Immunol 143, 1254-8 (1989)), is located away from the CR2 interaction site; therefore, this disease association is likely not directly related to CR2 binding but rather to other C3 functions.
Unexpectedly, a dimer of CR2 molecules is formed in the crystal through contacts between SCR1 domains (FIG. 1B). This CR2 dimer has a shape of two letter V's coming together head to head, with SCR1 facing SCR1 and SCR2 facing SCR2. No direct contacts occur between SCR2. This second CR2 is essentially a duplicate of the other CR2 molecule of the dimer that binds to C3d (FIG. 1B).
Description of the Structure of CR2
FIGS. 2A-2E show the structure of CR2. FIG. 2A is a ribbon representation of the CR2 SCR1 (in red) and SCR2 (in yellow) structures, showing the SCR fold and the packing of the two domains to form a V shape. The side chains of Cys residues that form intra-domain disulfide bonds are colored in cyan. Within each domain, the beta-strands B-E are labeled. A sugar residue GlcNAc (N-acetyl-glucosamine) is seen attached to N102 of SCR2. (Prepared using MOLSCRIPT). FIG. 2B shows the structure and packing interaction at the interface of CR2 SCR1 and SCR2 domains. Residues important for the tight packing between the two domains at the interface and the linker regions are shown. (Prepared with the program RIBBONS). FIG. 2C is a surface representation of the two-domain arrangement of CR2. (Prepared using GRASP). FIG. 2D shows the dimerization of CR2 through interactions between SCR1 of each molecule. Strand D2 and E1 of one CR2 molecule (red) packed against strand E1 and D2 of the second CR2 molecule in the dimer (blue). (Prepared using MOLSCRIPT). FIG. 2E shows a sequence alignment between human CR2 (SEQ ID NO:4) and mouse CR2 (mCR2) (SEQ ID NO:6). The first residue of CR2 (Gly) is changed to Ala in the structure due to PCR primer sequence. Secondary structure elements are shown above the corresponding aligned sequences. A black line represents coils and arrows represent beta-strands. The linker sequence between SCR1 and SCR2 is underlined. The residues whose side-chain or main-chain groups are involved in binding to C3d are indicated by *. The symbol ● indicates the two residues that, after mutating from mouse to human sequence, allow mCR2 to gain the ability to bind EBV gp350/220.
The CR2 structure described here contains residues 1-134 (SEQ ID NO:4; FIG. 2E, corresponding to residues 20-153 of the full length human CR2 (SEQ ID NO:1)) that fold into two SCR domains (FIG. 2A). Each domain shows a basic SCR fold: four anti-parallel beta-strands form a barrel-like beta-sheet. Strand D and E are separated into two segments, D1, D2 and E 1, E2. In addition to the hydrogen bonds that hold together the antiparallel beta-strands, two intra-domain disulfide bonds cross-link the coiled N-terminus to strand D2, and strand C to strand E2 (FIG. 2A). A well ordered N-linked GlcNAc (N-acetyl-glucosamine) residue is linked to Asn102 that is located at the tip of SCR2. The rest of the sugar residues were digested away by EndoH.
A very unique feature of the two domain CR2 structure is that the 8 amino acid linker makes a dramatic turn to allow the two SCR domains to pack against each other sideways (FIGS. 2A, 2B, 2C). Among the protein family containing SCR domains, the linkers connecting SCR domains are normally 3-5 amino acids in length, while an 8 amino acid linker is the longest known so far. The available structures of four SCR proteins containing two or 4 repeats (A. P. Wiles, et al., (1997), supra; P. N. Barlow, et al., (1993), supra; J. M. Casasnovas, et al., (1999), supra; R. Schwarzenbacher et al., (1999), supra) all have end-end packing between consecutive SCRs, and CR2 is thus the first to demonstrate extensive side-side packing.
In end-end packing, the adjacent domains could, in principle, adopt different rotations and bend angles relative to each other in different environments. Indeed, a commonly held concept is that SCRs are not absolutely fixed relative to each other but rather are allowed some freedom to move about this interface (P. N. Barlow, et al., (1993), ibid.; J. M. Casasnovas, et al., (1999), ibid.). However, the side-side packing of the CR2 SCR1 and SCR2 would not give the two domains freedom to adopt different orientations, unless some active process is involved to first separate the two domains. The interface between the two domains is mainly hydrophobic (FIG. 2B). Trp112, which is unique to CR2 SCR2 sequence and located on strand D2, plays a critical role in the packing by interacting with Ile39 and the main-chain from SCR1. Trp112 would be unfavorably exposed to the solvent if the two domains do not pack against each other sideways in this manner. In addition to Trp112, several other residues also play a role in the packing, including Pro121, His91, Leu39, and the carbon side chain of Glu64. The 8 amino acid linker, which contains mainly hydrophobic residues such as Tyr65, Phe66, Tyr69, also participates in the hydrophobic packing outside the two-domain interface, further solidifying the interactions between SCR1 and SCR2 (FIG. 2B). The area of the interface of the two SCRs is very extensive, covering almost half of the length of one SCR domain (FIG. 2C). Because of the above mentioned observations of other SCR proteins studied in the absence of their ligands, it will be interesting to see if the packing of these two CR2 SCR domains remains the same in the unliganded crystal and solution states.
Two CR2 molecules dimerize through SCR1-SCR1 contacts in the crystal structure (FIG. 2D). The contact is symmetrical, with the E1 strands from different molecules running anti-parallel to each other. The C-terminus of the E1 strand also contacts the C-terminus of the D2 strand of another molecule. The nature of the interaction is hydrogen bonds. In solution, however, the present inventors have found that CR2 behaves like a monomer under physiological buffer condition as assayed by gel filtration chromatography, though this is a technique that can only detect strong protein-protein interaction. It is possible that the dimer interaction only occurs in the crystal packing and is not of relevance. Another possibility, though, is that the interaction plays biological roles under physiologic conditions, but it is too weak to be detected in solution. Considering that CR2 receptors are largely constrained in the cell membrane to only lateral movement, no strong interactions would be necessary to dimerize.
Description of the Structure of the CR2-C3d Interface
FIGS. 3A-3F show the structure at the CR2-C3d interface. FIGS. 3A and 3B show the surface features of the interface area on C3d (in cyan) and CR2 molecule (in yellow). The shape of the interface of one molecule complements that of the other. (Prepared using GRASP). FIG. 3C shows the structure of the CR2 SCR2-C3d complex. The strands of CR2 (yellow) and helices of C3d (cyan) near the interface are labeled to provide a sense of orientation. (Prepared using MOLSCRIPT). FIGS. 3D and 3E show the detailed interactions between CR2 (in yellow) and C3d (in cyan) in two angles. Dashed lines represent hydrogen bonds between carbonyl oxygen atoms (in red), nitrogen atoms (in blue) of amino acid side chains or main chain, and water molecules (in purple). (Prepared using RIBBONS). FIG. 3F shows the human C3d sequence (SEQ ID NO:7) with secondary structure assigned on top of the corresponding sequences. The residues involved in CR2 binding are indicated by *. The residue D223 in C3F from (vs. N223 in C3S) is in pink. The residue N170 shown to weaken C3d-CR2 interaction after being mutated to Arg (see FIG. 4) is colored in red. N170 is also the only residue among those involved in CR2 binding that has its side chain in close contact with and pointing toward CR2.
One particularly salient feature of the interface is the shape-matching between C3d and CR2 interacting surfaces, as the protrusions on one molecule exactly match the pits and cavities on the other (FIGS. 3A & 3B). The total of the buried area in the interface is 1400 Å2. The contact interfaces between the receptor and C3d are neither hydrophobic nor particularly charged, but rather the well-fitted surface shapes from the two molecules come together to form the bonding contact in the complex. No major conformational difference is observed between the structures of free C3d and CR2-bound C3d, except for small movements (0.7-1.0 Å) of H3 (helix 3), H5, H7 and a few turns/loops on the surface.
In the CR2-C3d complex, no continuous stretch of residues on C3d participates in the interactions. Rather, residues that are separated in the linear sequence of C3d, but come together on the folded C3d, interact with CR2. Namely, the residues on the H3-H4 loop (the loop between helix 3 and 4), as well as H5, and H7 make contact with CR2 (FIG. 3C). On the CR2 part, however, a linear stretch of residues within SCR2 domain makes the contact with C3d. The B strand and B-C loop of SCR2 constitute the majority of the interactions with C3 d. The nature of the contacts involves elegant networks of hydrogen bonds, plus some hydrophobic and van der Waals interactions (FIGS. 3D & 3E).
It is particularly intriguing that only SCR2 directly contacts C3d. Previous studies have shown that both SCR1 and SCR2 are required for binding of polymeric forms of C3d to CR2 on cell membranes (C. A. Lowell, et al., J Exp Med 170, 1931-46 (1989); J. C. Carel, et al., J Biol Chem 265, 12293-9 (1990); K. R. Kalli, et al., J Immunol 147, 590-4 (1991)). The requirement for both domains to bind C3d on the cell surface may be due to the necessity of inter-SCR packing for stabilization of the SCR2 site, or alternatively the dimerization of CR2 mediated by SCR1 plays a necessary role on cell membranes, as discussed later.
Specificity of CR2-C3d Interaction
A few features about the CR2-C3d interactions are very striking. The first of such is the extensive use of main-chain carbonyl oxygen and nitrogen atoms in forming hydrogen bonds (H-bonds) between CR2 and C3d. This is particularly true on the C3d side, where the majority of the H-bond contributors on C3d come from main-chain carbonyl groups (FIGS. 3D & 3E). No side chains other than Asn170 on the C3d side are involved in the direct interaction with CR2. This observation likely explains the difficulties in previous efforts to accurately identify the residues that directly interact with CR2 by site-directed mutagenesis of C3d or by using C3d-derived inhibitory peptides. This interaction mode of CR2-C3d is reminiscent of MHC-antigen peptide recognition where MHC interacts with the main-chain atoms of the antigen peptide in order to allow the limited number of MHC to bind the unlimited variations of antigens for antigen presentation to T cells (P. J. Bjorkman, et al., Nature 329, 512-8 (1987)). However, in distinct contrast to the MHC-antigen peptide interaction, the binding between CR2 and C3d through main-chain atoms here does not sacrifice the specificity of the CR2 receptor for C3d ligand due to an additional pronounced shape-fitting requirement, as further evident in later discussion.
Another important feature concerns one of the major sites for binding CR2 on C3d at the C-terminal end of H5. Here four carbonyl groups, one each from Ile115, Leu116, Glu117 and Gln119 of C3d, are positioned in such a way that, collectively, they form an anion hole at the C-terminus of H5 (FIG. 3D). In the complex structure, this anion hole is occupied by the positively charged Arg84 of CR2 that is located on the B-C loop of SCR2 domain. In this case, CR2 Arg84 acts as a capping residue in-trans to seal the alpha-helical dipole moment at the C-terminus of H5 of C3d. This is in contrast to the usual observation in other protein-ligand interactions that involve an alpha-helical dipole moment, in which usually a negatively charged group interacts with the other protein through the positively charged N-terminus of a helix, possibly because of the easily accessible nitrogen atoms on the main-chain (C. Branden, J. Tooze, Introduction to Protein Structure (Garland Publishing, ed. second edition, 1999) pp. 16). However, for CR2 to interact with the negatively charged C-terminus of C3d H5 that is shielded by the particular conformation at the end of H5 and its neighboring structure, it requires CR2 to have a well-matched surface around the positively charged capping residue, providing specificity to the interaction. The sequences and conformation of SCR2 around Arg84 of CR2 provides such a surface that is well-matched with the complimentary part on C3d, and that provides the necessary specificity for the interaction with C3d.
The conformation of the C3d-binding region, the B-C loop of SCR2 domain on CR2, is thus also important for the specific binding of C3d (FIGS. 3C, 3D, 3E). As one means to accomplish this conformation, the B-C loop is held and presented on the surface of CR2 by strand B and C of SCR2 in such a way that Arg84 readily fits into the anion hole at the end of the C3d H5. The residue after Arg84 is Gly85, which does not have a side chain that would interfere with the CR2 and C3d interface interaction. The side chain of Ser86 forms an H-bond with a carbonyl oxygen from the H3-H4 loop of C3d through a water molecule (FIG. 3E). Other residues around Arg84 have side chains pointing away from the interface and use either their main-chain carbonyl oxygen or nitrogen atoms to form H-bonds with C3d. This mode of interaction from the CR2 side predicts that the basic capping residue Arg84, as well as Ser86, are likely the most important residues on this interaction surface, as long as they are presented in a correct conformation.
In this regard, of importance also is a sequence comparison between human CR2 and mouse CR2 (mCR2), both of which bind C3d with similar affinity (D. R. Martin et al., J Exp Med 174, 1299-311 (1991); J. D. Fingeroth et al., Proc Natl Acad Sci USA 86, 242-6 (1989)). The SCR1-2 region of human CR2 is represented herein as SEQ ID NO:4 and is compared to the corresponding SCR1-2 region of mouse CR2 (SEQ ID NO:6). At the C3d-interacting interface the B-C loop of mCR2 SCR2 has a basic residue Lys in place of Arg84, while Gly85 and Ser86 are conserved (positions given relative to SEQ ID NO:4). Five other amino acids around Arg84 on the B-C loop are not highly conserved (FIG. 3F). The complex structure reveals, however, that the side chains of these five non-conserved residues do not participate in the CR2-C3d interaction. Importantly, Lys84 in mouse (SEQ ID NO:6) could replace Arg84 (SEQ ID NO:4) as the trans-capping residue for H5. Of interest, outside of the 5 amino acids around Lys84 (SEQ ID NO:6), there are stretches of amino acids that are the most highly conserved between human CR2 and mCR2 (FIG. 2E). These highly conserved segments likely play an important role in presenting the B-C loop in a correct conformation for specific C3d binding to mCR2.
Another feature of the complex structure is the participation of several water molecules in the interaction (FIGS. 3D & 3E). Well-ordered water molecules participate in the formation of some H-bonds between CR2 and C3d, acting as water “glue” between the two proteins. Extensive participation of water molecules in mediating receptor-ligand binding is also seen in the interaction between CAR receptor (coxsacki-adenovirus receptor) and adenovirus knob protein (M. C. Bewley et al., Science 286, 1579-83 (1999)).
A six-coordinated Zn atom is present on the edge of the interface between CR2 and C3d (FIG. 3D). Glu117 of C3d is the only amino acid side chain that participates in the coordination. The other four coordinates are mediated by water molecules, through which H-bond connections are formed with CR2 main-chain. The contribution of the Zn coordination to the CR2-C3d binding is likely to be insignificant, because no obvious change in formation of the CR2-C3d complex can be detected in buffers with/without Zn ion, or when using a C3d mutant containing a Glu117 to Ala mutation (see next section). In addition, the binding of CR2 with C3d does not demonstrate a known cation dependence.
Example 2
The following example describes the construction of C3d mutants that affect CR2 binding.
Based on the complex structure, mutagenesis of C3d around the interface to disrupt CR2 binding was predicted to be difficult. This is because the interaction between CR2 and C3d involves mostly main-chain H-bonding, and the side chain residues play relatively small roles in the binding. However, to confirm the accuracy of the CR2-C3d interaction seen in this co-crystal structure, two informative C3d mutants were constructed. In mutant 170 (mt170; SEQ ID NO:8), residue Asn170 was changed to Arg. Asn170 is located on H7 of C3d and is the only residue on C3d that more or less points directly toward CR2 in the interface (FIG. 3E). Asn170 also packs directly with Tyr81 of CR2 as well as forms an H-bond with CR2 (Lys 100) through a water molecule (FIG. 3E). In solution, this mutant protein behaved very similarly like the wild type C3d and showed the same apparent molecular weight as the wild type in gel-filtration chromatography, suggesting correct folding. In the binding experiment assayed by native gel shift, however, the interaction between mt170 protein and CR2 was clearly less strong comparing with that between wild type C3d and CR2 (FIG. 4A, lanes 1-4 versus 9-12).
Briefly, FIGS. 4A and 4B show a native gel shift assay of the binding between CR2 and C3d wild type (wt) or mutants (mt). FIG. 4A shows a 6% native polyacrylamide gel of C3d alone (lanes 1, 5, and 9) or C3d plus increasing amount of CR2 (other lanes). mt170 C3d alone (lane 9) migrated slower than both wt (lane 1) and mt115 due to the introduction of a positively charged Arg residue at N170 (lane 5). CR2 alone does not enter the gel in this system. The C3d band will be shifted to the upper part of the gel when it forms a complex with CR2. The C3d concentration (for both wt and mt) is the same throughout lanes 1-12. The CR2 added to each corresponding lanes for wt and mt C3d was also the same, e.g. same amount of CR2 was added to the C3d samples in lane 2, 6 and 10, or in lanes 4, 8 and 12. In the lanes with the highest CR2 concentration (lanes 4, 8, 12), the bands of C3d wt and mt115 were completely shifted to the complex form (lane 4, 8), while mt170 (migrated slower than the wt or mt115) still contains an obvious C3d band (lane 12). These results indicate that the interaction between mtl 70 and CR2 is weaker than that between the wt or mt115 with CR2. The same conclusion can be drawn by comparing the intensity of the bands of the complexes containing the wt, mt115 and mtl 70. FIG. 4B is a graphical representation showing the intensity changes of the complex bands (measured by densitometry) as CR2 concentration increases from lanes 2 to 4 (wt), or lanes 6 to 8 (mt115), or lanes 10 to 12 (mt170). The result clearly shows that mt170 has less apparent affinity for CR2 than wt and mt115.
The result shown in this experiment is consistent with that predicted by the interaction interface seen in the complex structure. In another mutant of C3d (mt115), although three residues: Gln105, Leu116 and Glu117, were mutated to Ala, the mutant protein has no obvious effect on CR2 binding (FIG. 4A, lanes 1-4 versus 5-8). This is consistent with the complex structure in that all these three residues, even though located within the interface area, have their side chains pointing sideways and are not involved in the direct contact with CR2. The side chain of Glu117, however, participates in the Zn coordination. This mutant showed that Zn coordination by Glu117 does not contribute significantly to CR2-C3d binding. The coordinated Zn in the structure, therefore, could simply come from the crystallization buffer which contained ZnAcetate.
FIG. 4C demonstrates the results of a competitive ELISA analysis using informative C3d mutants. In this analysis, C3d mutant Gln105Ala, Leu116Ala, Glu117Ala (designated Mt115 in FIGS. 4A and 4B and mut1 in FIG. 4C) manifests an equal ability as wild type C3d to block the binding of soluble full length CR2 to plate-bound wild type C3d. Therefore, mutations of these two residues which utilize only the main-chain atoms to interact with CR2 did not have an effect, which is predicted by the X-ray structure. Two other informative mutants, one Asn170Arg (designated mt170 in FIGS. 4A and 4B and mut2 in FIG. 4C, also represented herein as SEQ ID NO:8) and the other a new mutant Asn170Ala, Ile115Arg, Leu116Arg (designated mut4 in FIG. 4C, also represented herein as SEQ ID NO:9), are both unable to effectively block soluble CR2 binding to plate-bound wild type C3d. Therefore, we conclude that two mutations of C3d involving Asn170, whose side-chain interacts with CR2 directly, result in substantially decreased binding to CR2.
Of interest, the mode of interaction seen here is very different from that previously predicted by C3d mutants and C3d-derived peptides (J. D. Lambris et al., Proc Natl Acad Sci USA 82, 4235-9 (1985); R. J. Diefenbach et al., J Immunol 154, 2303-20 (1995); L. Clemenza et al., J Immunol 165, 3839-48 (2000)). Close examination of the complex structure, however, can explain some of the previous mutagenesis results. For example, residues Asp163, Ile164, and Glu166 of C3d, which affect CR2-binding after being mutated to Ala (Clemenza et al., 2000, ibid.), are located on H6 that is juxtaposed to H5 where the major CR2-recognizing anion hole is positioned (FIG. 3D). Based on the structure, it is obvious that mutations of some of the residues on H6 (such as Ile164) of C3d will affect the relative positions of H6 and H5, which may account for the reduced interaction with CR2. The other group of mutations on C3d (Asp36, Glu37, Glu39) reported to weaken CR2 binding (Clemenza et al., 2000, ibid.) are located right next to the N and C termini, and the present inventors' findings cannot account for those results. However, it is of note that this particular site would be juxtaposed to additional large peptides derived from iC3b, and these additional peptides in iC3b would present a steric hindrance for the interaction with CR2 if these residues were to be involved in CR2 binding. The present inventors' observation that CR2 contacts C3d on the opposite side from the N and C termini removes that concern and is more consistent with the observed high affinity binding of CR2 to iC3b.
Example 3
The following example describes the inhibition of CR2-C3d interaction by CR2-derived peptides.
Based on the structure of the CR2-C3d complex, the results from previously reported CR2 peptide inhibition and monoclonal antibody assays can now be explained. In the peptide inhibition tests using short synthetic peptides covering all of CR2 SCR1 and S CR2, peptides from two regions were shown to inhibit CR2-C3 binding (H. Molina, et al., J Immunol 154, 5426-35 (1995)). One of them contains sequences that are located right on the interaction interface of CR2 seen in the complex structure, namely the sequences from the B strand and B-C loop of SCR2 (FIGS. 3D & 3E). This independent result strongly supports the complex structure described by the present invention. However, the other peptide located on the B strand of SCR1 also showed a similar inhibition effect as the first one. Close examination of this fragment on CR2 structure within the SCR1 domain revealed a very similar 3-dimensional arrangement of these residues as the one on SCR2, but the sequence is in part reversed. Part of the C3d-binding sequences on the SCR2 B-C loop is 83-IRGSTP-88. The peptide from SCR1 that also has inhibitory effects has a sequence of 11-LNGRIS-16 (H. Molina et aI., 1995, ibid). Similarities are apparent between these two peptides if one of the sequences is reversed. Specifically, at the 3-dimensional structure level, the conformation of -NGRI- of the SCR1 peptide looks like a reverse duplicate of -IRGS- on SCR2. However, in a folded CR2 molecule, the structure of the neighboring residues around the peptide on SCR1 restricts the accessibility of the sequences within the peptide, especially the Arg residue. Therefore, this part of CR2 should not be able to bind C3d unless some major conformational switch occurred to expose the segment in a fashion similar to it in a short peptide state.
Example 4
The following example describes the inhibition of CR2-C3d binding by anti-CR2 monoclonal antibodies.
The previously mapped epitope positions on CR2 for inhibitory monoclonal antibodies also support the interaction sites seen in the CR2-C3d complex structure. Two inhibitory antibodies, OKB7 (P. E. Rao et al., Cell Immunol 93, 549-55 (1985)) and FE8 (W. M. Prodinger, et al., J Immunol 161, 4604-10 (1998)), have epitopes positioned right next to the C3d binding region (FIGS. 5A & 5B). The present inventors have also created three new monoclonal antibodies, denoted mAb171, mAb1048 and mAb629, that inhibit the specific binding of CR2 to C3d. All three have mapped epitopes on CR2 lying right within the C3d-interacting area (FIG. 5A, blue area). Samples of the hybridoma cell lines UCO-Anti-CR2-171, UCO-Anti-CR2-1048, and UCO-Anti-CR2-629, which produce monoclonal antibodies mAb171, mAb1048, and mAb629, respectively, have been deposited under the provisions of the Budapest Treaty with the American Type Culture Collection (ATCC®), 10801 University Blvd., Manassas, Va., 20110, on Sep. 20, 2011. The hybridomas have been assigned ATCC® Patent Deposit Designations PTA-12091, Patent Deposit Designations PTA-12091, PTA-12093, and PTA-12092, respectively.
FIGS. 5A and 5B show the localization of the epitopes of anti-CR2 monoclonal antibodies (mAb) on the CR2 surface. These figures are two views with a 180 degree rotation to each other. The C3d binding site is labeled. All of the mAbs inhibit the interactions between CR2 and C3d to some extent. mAb 171, together with 1048 and 629 (colored in blue) have epitopes located within the C3d-binding area. OKB7 (Rao et al., 1985, ibid) (colored in red) and FE8 (Prodinger et al., 1998, ibid) (colored in pink) also have epitopes right next to the C3d-binding region. The green spots indicate the locations where mutations from mCR2 to hCR2 sequence allow mCR2 to gain the ability to bind EBV gp350/220. The surfaces around these two sites are likely located within the interface between CR2 and gp350/220.
Example 5
The following example describes a potential gp350/220 binding region in CR2.
Previous evidence suggests that CR2 binds to C3d and EBV gp350/220 with overlapping but distinct sites (D. R. Martin et al., J Exp Med 174, 1299-311 (1991); H. Molina et al., J Biol Chem 266, 12173-9 (1991); D. R. Martin et al., J Vivol 68, 4716-26 (1994); H. Molina et al., J Immunol 154, 5426-35 (1995)). One instrumental result in this regard has been the ability to transform by point mutation mCR2 into a form capable of binding gp350/220. In this regard, mCR2 and CR2 both bind C3d, but mCR2 does not bind EBV or gp350/220. However, changing the sequence of mCR2 at two amino acids (Pro16 to Ser and Thr69 to Tyr, FIG. 2E) allowed mCR2 to gain the ability to bind EBV (D. R. Martin et al., J Virol 68, 4716-26 (1994)). Because of this, the locations of the two mutated residues are likely to be involved in gp350/220 binding. Of interest, these locations mapped on the CR2 surface (green patch on FIGS. 5A & 5B) are separated from the blue area that interacts with C3d in the complex structure of the present invention.
Example 6
The following example describes a structure model of CR2 in complex with C3d on the cell surface.
Based on the complex structure, the present inventors propose a model of CR2 binding to C3d or iC3b on the cell surface (FIG. 6). FIG. 6A is a surface representation of the model containing a dimer of CR2 SCR1 and SCR2 that bind to C3d on each receptor. The dimer contact is through SCR1 only, as seen in the crystal structure. FIG. 6B is a diagram of C3d-antigen cross-linking CR2 (as dimers) and BCR on the cell surface. The dimer form of CR2, as opposed to the monomer, in complex with CD19/CD81 permits the cross-linking of multiple CR2 by C3d-antigen to greatly increase the local concentration of CR2/CD19/CD81
In this model, CR2 in complex with C3d molecules exists as a dimer through SCR1-SCR1 contact (FIGS. 6A & 6B). C3d- or iC3b-bound antigen interacts with SCR2 in such a way that the site of ester-linkage with the antigen (residue Q20) is pointing in the lateral direction (FIG. 6A). This orientation allows CR2 maximum capabilities to interact with C3 bound to many shapes and sizes of antigens. In addition, though, there is also the opportunity for the antigen to cross-link a neighboring CR2 dimer with a second C3d/iC3b attached to it. Repeat of this interaction could cross-link many CR2/CD19/CD81 and BCR molecules, with the potential of greatly amplifying down-stream signal transduction. Of interest, previous reports of C3d acting as a molecular adjuvant showed that two or three C3d attached to hen egg lysozyme (HEL) enhanced the IgG1 humoral response to HEL by 1,000 to 10,000 times, but one C3d had a suppressive effect (P. W. Dempsey et al., Science 271, 348-50 (1996)). The model in FIG. 6B provides one potential molecular explanation for this observation. Multiple CR2 dimers can be brought together by two (or more) C3d linked to an antigen, but only two (or three) CR2 monomers can be cross-linked together. Thus, the CR2 dimer model, in contrast to a monomer, allows a dramatic increase of cross-linked CR2/CD19/CD81 and BCR molecules through binding to the antigen with two or more C3d attached, which could account for the 10.000-fold enhancement of the observed immune response.







TABLE 2





lists the atomic coordinates for a crystal of SCR1-2 of human CR2


(SEQ ID NO: 4) bound to C3d (SEQ ID NO: 7).


























ATOM
1
CB
ALA
A
1
113.978
74.531
51.463
1.00
24.47
A


ATOM
2
C
ALA
A
1
113.746
72.085
52.085
1.00
26.48
A


ATOM
3
O
ALA
A
1
112.896
72.106
52.942
1.00
30.12
A


ATOM
4
N
ALA
A
1
115.853
72.884
51.119
1.00
42.70
A


ATOM
5
CA
ALA
A
1
114.718
73.256
51.995
1.00
33.68
A


ATOM
6
N
LEU
A
2
113.868
71.065
51.232
1.00
24.18
A


ATOM
7
CA
LEU
A
2
112.952
69.905
51.320
1.00
27.55
A


ATOM
8
CB
LEU
A
2
112.148
69.728
50.001
1.00
23.79
A


ATOM
9
CG
LEU
A
2
110.907
68.774
50.002
1.00
26.21
A


ATOM
10
CD1
LEU
A
2
109.785
69.339
50.877
1.00
20.38
A


ATOM
11
CD2
LEU
A
2
110.365
68.568
48.562
1.00
22.09
A


ATOM
12
C
LEU
A
2
113.635
68.549
51.642
1.00
27.27
A


ATOM
13
O
LEU
A
2
114.746
68.266
51.172
1.00
22.30
A


ATOM
14
N
ASP
A
3
112.963
67.734
52.455
1.00
23.38
A


ATOM
15
CA
ASP
A
3
113.426
66.393
52.758
1.00
23.91
A


ATOM
16
CB
ASP
A
3
114.304
66.327
54.028
1.00
20.20
A


ATOM
17
CG
ASP
A
3
113.619
66.825
55.279
1.00
22.03
A


ATOM
18
OD1
ASP
A
3
112.357
66.765
55.433
1.00
20.42
A


ATOM
19
OD2
ASP
A
3
114.366
67.274
56.160
1.00
28.30
A


ATOM
20
C
ASP
A
3
112.201
65.488
52.875
1.00
23.39
A


ATOM
21
O
ASP
A
3
111.047
65.953
52.784
1.00
18.32
A


ATOM
22
N
ALA
A
4
112.438
64.194
53.060
1.00
24.33
A


ATOM
23
CA
ALA
A
4
111.330
63.243
53.164
1.00
23.20
A


ATOM
24
CB
ALA
A
4
111.854
61.819
53.288
1.00
17.28
A


ATOM
25
C
ALA
A
4
110.291
63.499
54.261
1.00
19.39
A


ATOM
26
O
ALA
A
4
109.113
63.369
53.958
1.00
16.69
A


ATOM
27
N
GLU
A
5
110.691
63.841
55.491
1.00
16.50
A


ATOM
28
CA
GLU
A
5
109.701
64.065
56.548
1.00
22.14
A


ATOM
29
CB
GLU
A
5
110.301
64.257
57.982
1.00
23.56
A


ATOM
30
CG
GLU
A
5
110.935
65.596
58.263
1.00
38.23
A


ATOM
31
CD
GLU
A
5
110.138
66.605
59.095
1.00
31.60
A


ATOM
32
OE1
GLU
A
5
109.004
66.397
59.598
1.00
31.04
A


ATOM
33
OE2
GLU
A
5
110.719
67.671
59.253
1.00
44.56
A


ATOM
34
C
GLU
A
5
108.853
65.262
56.208
1.00
20.89
A


ATOM
35
O
GLU
A
5
107.637
65.242
56.419
1.00
22.09
A


ATOM
36
N
ARG
A
6
109.470
66.300
55.662
1.00
17.73
A


ATOM
37
CA
ARG
A
6
108.703
67.491
55.302
1.00
23.39
A


ATOM
38
CB
ARG
A
6
109.618
68.614
54.815
1.00
25.00
A


ATOM
39
CG
ARG
A
6
110.433
69.194
55.948
1.00
24.66
A


ATOM
40
CD
ARG
A
6
111.430
70.240
55.488
1.00
30.21
A


ATOM
41
NE
ARG
A
6
112.143
70.710
56.662
1.00
31.75
A


ATOM
42
CZ
ARG
A
6
113.044
71.686
56.675
1.00
37.88
A


ATOM
43
NH1
ARG
A
6
113.377
72.333
55.558
1.00
32.11
A


ATOM
44
NH2
ARG
A
6
113.608
72.015
57.830
1.00
38.98
A


ATOM
45
C
ARG
A
6
107.696
67.156
54.219
1.00
25.83
A


ATOM
46
O
ARG
A
6
106.532
67.558
54.304
1.00
20.78
A


ATOM
47
N
LEU
A
7
108.122
66.409
53.205
1.00
19.13
A


ATOM
48
CA
LEU
A
7
107.179
66.068
52.143
1.00
17.13
A


ATOM
49
CB
LEU
A
7
107.916
65.488
50.945
1.00
16.82
A


ATOM
50
CG
LEU
A
7
106.984
65.041
49.805
1.00
23.63
A


ATOM
51
CD1
LEU
A
7
106.086
66.207
49.303
1.00
18.87
A


ATOM
52
CD2
LEU
A
7
107.870
64.538
48.676
1.00
25.78
A


ATOM
53
C
LEU
A
7
106.103
65.075
52.615
1.00
19.67
A


ATOM
54
O
LEU
A
7
104.935
65.191
52.246
1.00
20.24
A


ATOM
55
N
LYS
A
8
106.489
64.099
53.427
1.00
21.02
A


ATOM
56
CA
LYS
A
8
105.521
63.110
53.928
1.00
24.15
A


ATOM
57
CB
LYS
A
8
106.205
62.100
54.869
1.00
26.98
A


ATOM
58
CG
LYS
A
8
105.339
60.838
55.146
1.00
29.12
A


ATOM
59
CD
LYS
A
8
105.997
59.844
56.108
1.00
48.06
A


ATOM
60
CE
LYS
A
8
107.390
59.397
55.608
1.00
61.21
A


ATOM
61
NZ
LYS
A
8
108.117
58.353
56.469
1.00
69.61
A


ATOM
62
C
LYS
A
8
104.281
63.720
54.630
1.00
22.77
A


ATOM
63
O
LYS
A
8
103.163
63.203
54.499
1.00
16.94
A


ATOM
64
N
HIS
A
9
104.465
64.778
55.412
1.00
17.13
A


ATOM
65
CA
HIS
A
9
103.303
65.454
56.034
1.00
23.94
A


ATOM
66
CB
HIS
A
9
103.759
66.675
56.838
1.00
15.51
A


ATOM
67
CG
HIS
A
9
104.457
66.331
58.109
1.00
25.05
A


ATOM
68
CD2
HIS
A
9
105.755
66.445
58.478
1.00
22.49
A


ATOM
69
ND1
HIS
A
9
103.792
65.831
59.209
1.00
24.32
A


ATOM
70
CE1
HIS
A
9
104.649
65.654
60.200
1.00
26.37
A


ATOM
71
NE2
HIS
A
9
105.851
66.015
59.786
1.00
31.51
A


ATOM
72
C
HIS
A
9
102.268
65.959
55.002
1.00
24.84
A


ATOM
73
O
HIS
A
9
101.161
66.278
55.375
1.00
23.11
A


ATOM
74
N
LEU
A
10
102.634
66.074
53.721
1.00
18.19
A


ATOM
75
CA
LEU
A
10
101.670
66.538
52.730
1.00
22.98
A


ATOM
76
CB
LEU
A
10
102.370
67.210
51.534
1.00
24.09
A


ATOM
77
CG
LEU
A
10
103.171
68.492
51.885
1.00
30.55
A


ATOM
78
CD1
LEU
A
10
103.625
69.205
50.648
1.00
29.82
A


ATOM
79
CD2
LEU
A
10
102.285
69.415
52.701
1.00
39.35
A


ATOM
80
C
LEU
A
10
100.789
65.391
52.237
1.00
30.17
A


ATOM
81
O
LEU
A
10
99.875
65.616
51.442
1.00
31.15
A


ATOM
82
N
ILE
A
11
101.065
64.159
52.663
1.00
20.98
A


ATOM
83
CA
ILE
A
11
100.194
63.053
52.229
1.00
22.19
A


ATOM
84
CB
ILE
A
11
101.002
61.747
52.060
1.00
27.01
A


ATOM
85
CG2
ILE
A
11
100.044
60.547
51.788
1.00
29.34
A


ATOM
86
CG1
ILE
A
11
101.984
61.940
50.863
1.00
21.97
A


ATOM
87
CD1
ILE
A
11
103.039
60.904
50.736
1.00
31.22
A


ATOM
88
C
ILE
A
11
99.082
62.956
53.269
1.00
27.58
A


ATOM
89
O
ILE
A
11
99.300
62.542
54.411
1.00
26.44
A


ATOM
90
N
VAL
A
12
97.890
63.403
52.888
1.00
23.12
A


ATOM
91
CA
VAL
A
12
96.760
63.400
53.803
1.00
22.74
A


ATOM
92
CB
VAL
A
12
96.392
64.814
54.185
1.00
33.31
A


ATOM
93
CG1
VAL
A
12
97.530
65.452
55.004
1.00
34.31
A


ATOM
94
CG2
VAL
A
12
96.105
65.600
52.928
1.00
24.64
A


ATOM
95
C
VAL
A
12
95.487
62.726
53.265
1.00
28.78
A


ATOM
96
O
VAL
A
12
95.316
62.525
52.061
1.00
25.51
A


ATOM
97
N
THR
A
13
94.577
62.396
54.174
1.00
22.73
A


ATOM
98
CA
THR
A
13
93.348
61.747
53.787
1.00
24.33
A


ATOM
99
CB
THR
A
13
92.824
60.893
54.947
1.00
28.46
A


ATOM
100
OG1
THR
A
13
93.834
59.921
55.298
1.00
37.06
A


ATOM
101
CG2
THR
A
13
91.503
60.186
54.540
1.00
22.36
A


ATOM
102
C
THR
A
13
92.328
62.811
53.443
1.00
19.70
A


ATOM
103
O
THR
A
13
92.043
63.649
54.254
1.00
18.28
A


ATOM
104
N
PRO
A
14
91.760
62.781
52.239
1.00
23.16
A


ATOM
105
CD
PRO
A
14
92.057
61.889
51.100
1.00
27.40
A


ATOM
106
CA
PRO
A
14
90.765
63.803
51.883
1.00
23.69
A


ATOM
107
CB
PRO
A
14
90.675
63.699
50.355
1.00
20.22
A


ATOM
108
CG
PRO
A
14
90.959
62.232
50.106
1.00
26.75
A


ATOM
109
C
PRO
A
14
89.406
63.564
52.509
1.00
22.95
A


ATOM
110
O
PRO
A
14
89.009
62.415
52.725
1.00
20.64
A


ATOM
111
N
SER
A
15
88.719
64.651
52.845
1.00
19.26
A


ATOM
112
CA
SER
A
15
87.348
64.575
53.337
1.00
25.94
A


ATOM
113
CB
SER
A
15
87.295
64.124
54.797
1.00
25.47
A


ATOM
114
OG
SER
A
15
88.078
64.979
55.578
1.00
28.02
A


ATOM
115
C
SER
A
15
86.638
65.928
53.187
1.00
26.13
A


ATOM
116
O
SER
A
15
87.227
66.923
52.758
1.00
26.66
A


ATOM
117
N
GLY
A
16
85.364
65.948
53.556
1.00
28.55
A


ATOM
118
CA
GLY
A
16
84.593
67.167
53.481
1.00
25.28
A


ATOM
119
C
GLY
A
16
83.748
67.259
52.225
1.00
23.22
A


ATOM
120
O
GLY
A
16
83.667
66.314
51.428
1.00
20.49
A


ATOM
121
N
ALA
A
17
83.122
68.421
52.072
1.00
21.03
A


ATOM
122
CA
ALA
A
17
82.258
68.742
50.952
1.00
19.04
A


ATOM
123
CB
ALA
A
17
81.288
69.898
51.356
1.00
17.25
A


ATOM
124
C
ALA
A
17
83.051
69.097
49.692
1.00
19.33
A


ATOM
125
O
ALA
A
17
84.271
68.928
49.643
1.00
22.08
A


ATOM
126
N
GLY
A
18
82.356
69.566
48.668
1.00
17.15
A


ATOM
127
CA
GLY
A
18
82.983
69.869
47.376
1.00
17.27
A


ATOM
128
C
GLY
A
18
84.233
70.743
47.300
1.00
21.54
A


ATOM
129
O
GLY
A
18
84.993
70.629
46.350
1.00
16.56
A


ATOM
130
N
GLU
A
19
84.419
71.653
48.248
1.00
18.40
A


ATOM
131
CA
GLU
A
19
85.629
72.488
48.258
1.00
22.07
A


ATOM
132
CB
GLU
A
19
85.302
73.929
48.683
1.00
14.92
A


ATOM
133
CG
GLU
A
19
84.450
74.722
47.650
1.00
18.57
A


ATOM
134
CD
GLU
A
19
84.034
76.086
48.184
1.00
21.03
A


ATOM
135
OE1
GLU
A
19
84.654
76.565
49.162
1.00
20.93
A


ATOM
136
OE2
GLU
A
19
83.096
76.694
47.639
1.00
19.11
A


ATOM
137
C
GLU
A
19
86.679
71.889
49.203
1.00
18.24
A


ATOM
138
O
GLU
A
19
87.865
71.779
48.822
1.00
17.93
A


ATOM
139
N
GLN
A
20
86.235
71.519
50.415
1.00
15.20
A


ATOM
140
CA
GLN
A
20
87.084
70.923
51.502
1.00
23.25
A


ATOM
141
CB
GLN
A
20
86.217
70.526
52.751
1.00
23.39
A


ATOM
142
CG
GLN
A
20
85.294
71.692
53.342
1.00
43.73
A


ATOM
143
CD
GLN
A
20
84.079
71.273
54.296
1.00
42.22
A


ATOM
144
OE1
GLN
A
20
83.301
70.366
53.989
1.00
38.04
A


ATOM
145
NE2
GLN
A
20
83.924
71.988
55.410
1.00
33.68
A


ATOM
146
C
GLN
A
20
87.816
69.660
50.976
1.00
24.37
A


ATOM
147
O
GLN
A
20
88.996
69.451
51.237
1.00
17.83
A


ATOM
148
N
ASN
A
21
87.100
68.832
50.225
1.00
15.50
A


ATOM
149
CA
ASN
A
21
87.677
67.616
49.677
1.00
18.10
A


ATOM
150
CB
ASN
A
21
86.601
66.790
48.967
1.00
13.23
A


ATOM
151
CG
ASN
A
21
87.145
65.457
48.485
1.00
23.30
A


ATOM
152
OD1
ASN
A
21
87.490
64.598
49.294
1.00
17.88
A


ATOM
153
ND2
ASN
A
21
87.251
65.294
47.168
1.00
17.44
A


ATOM
154
C
ASN
A
21
88.847
67.891
48.721
1.00
21.18
A


ATOM
155
O
ASN
A
21
89.789
67.088
48.658
1.00
21.46
A


ATOM
156
N
MET
A
22
88.787
69.004
47.971
1.00
14.28
A


ATOM
157
CA
MET
A
22
89.850
69.360
47.039
1.00
13.52
A


ATOM
158
CB
MET
A
22
89.330
70.305
45.939
1.00
19.05
A


ATOM
159
CG
MET
A
22
88.285
69.647
45.064
1.00
20.17
A


ATOM
160
SD
MET
A
22
88.876
68.159
44.293
1.00
28.63
A


ATOM
161
CE
MET
A
22
90.211
68.915
43.271
1.00
21.96
A


ATOM
162
C
MET
A
22
91.028
69.995
47.767
1.00
15.63
A


ATOM
163
O
MET
A
22
92.159
69.896
47.328
1.00
16.97
A


ATOM
164
N
ILE
A
23
90.743
70.644
48.886
1.00
11.88
A


ATOM
165
CA
ILE
A
23
91.784
71.242
49.714
1.00
19.19
A


ATOM
166
CB
ILE
A
23
91.126
72.029
50.870
1.00
13.50
A


ATOM
167
CG2
ILE
A
23
92.159
72.357
51.971
1.00
13.80
A


ATOM
168
CG1
ILE
A
23
90.476
73.296
50.297
1.00
16.98
A


ATOM
169
CD1
ILE
A
23
89.807
74.203
51.351
1.00
17.93
A


ATOM
170
C
ILE
A
23
92.597
70.046
50.235
1.00
22.66
A


ATOM
171
O
ILE
A
23
93.843
70.053
50.257
1.00
18.47
A


ATOM
172
N
GLY
A
24
91.892
68.990
50.628
1.00
15.67
A


ATOM
173
CA
GLY
A
24
92.583
67.804
51.110
1.00
17.85
A


ATOM
174
C
GLY
A
24
93.291
67.056
50.000
1.00
21.53
A


ATOM
175
O
GLY
A
24
94.439
66.616
50.138
1.00
16.68
A


ATOM
176
N
MET
A
25
92.631
66.918
48.864
1.00
18.50
A


ATOM
177
CA
MET
A
25
93.234
66.170
47.767
1.00
15.73
A


ATOM
178
CB
MET
A
25
92.153
65.985
46.703
1.00
21.35
A


ATOM
179
CG
MET
A
25
92.469
65.151
45.529
1.00
26.92
A


ATOM
180
SD
MET
A
25
90.966
64.905
44.498
1.00
24.37
A


ATOM
181
CE
MET
A
25
91.718
63.617
43.360
1.00
17.81
A


ATOM
182
C
MET
A
25
94.511
66.832
47.185
1.00
23.64
A


ATOM
183
O
MET
A
25
95.432
66.146
46.757
1.00
17.81
A


ATOM
184
N
THR
A
26
94.577
68.164
47.180
1.00
17.90
A


ATOM
185
CA
THR
A
26
95.736
68.856
46.607
1.00
18.87
A


ATOM
186
CB
THR
A
26
95.629
70.395
46.847
1.00
17.83
A


ATOM
187
OG1
THR
A
26
94.436
70.869
46.218
1.00
17.41
A


ATOM
188
CG2
THR
A
26
96.851
71.139
46.262
1.00
16.75
A


ATOM
189
C
THR
A
26
97.149
68.423
47.052
1.00
11.10
A


ATOM
190
O
THR
A
26
97.990
68.069
46.229
1.00
17.69
A


ATOM
191
N
PRO
A
27
97.418
68.448
48.353
1.00
13.60
A


ATOM
192
CD
PRO
A
27
96.664
68.937
49.532
1.00
11.14
A


ATOM
193
CA
PRO
A
27
98.788
68.049
48.696
1.00
17.00
A


ATOM
194
CB
PRO
A
27
98.861
68.267
50.218
1.00
12.53
A


ATOM
195
CG
PRO
A
27
97.406
68.312
50.676
1.00
15.76
A


ATOM
196
C
PRO
A
27
99.193
66.662
48.277
1.00
21.64
A


ATOM
197
O
PRO
A
27
100.324
66.455
47.840
1.00
19.29
A


ATOM
198
N
THR
A
28
98.273
65.709
48.393
1.00
16.44
A


ATOM
199
CA
THR
A
28
98.607
64.333
48.046
1.00
17.14
A


ATOM
200
CB
THR
A
28
97.488
63.396
48.522
1.00
18.78
A


ATOM
201
OG1
THR
A
28
97.290
63.620
49.926
1.00
16.04
A


ATOM
202
CG2
THR
A
28
97.822
61.967
48.263
1.00
20.93
A


ATOM
203
C
THR
A
28
98.827
64.211
46.567
1.00
19.51
A


ATOM
204
O
THR
A
28
99.799
63.572
46.116
1.00
17.24
A


ATOM
205
N
VAL
A
29
97.957
64.844
45.781
1.00
16.43
A


ATOM
206
CA
VAL
A
29
98.164
64.762
44.346
1.00
16.98
A


ATOM
207
CB
VAL
A
29
97.035
65.430
43.524
1.00
21.71
A


ATOM
208
CG1
VAL
A
29
97.421
65.427
41.999
1.00
14.09
A


ATOM
209
CG2
VAL
A
29
95.731
64.619
43.708
1.00
22.05
A


ATOM
210
C
VAL
A
29
99.474
65.393
43.939
1.00
24.43
A


ATOM
211
O
VAL
A
29
100.260
64.768
43.179
1.00
19.52
A


ATOM
212
N
ILE
A
30
99.767
66.608
44.420
1.00
21.18
A


ATOM
213
CA
ILE
A
30
101.022
67.194
43.950
1.00
21.90
A


ATOM
214
CB
ILE
A
30
101.165
68.700
44.228
1.00
25.58
A


ATOM
215
CG2
ILE
A
30
100.124
69.422
43.519
1.00
45.44
A


ATOM
216
CG1
ILE
A
30
101.109
68.995
45.713
1.00
20.88
A


ATOM
217
CD1
ILE
A
30
102.385
68.792
46.400
1.00
43.61
A


ATOM
218
C
ILE
A
30
102.227
66.495
44.510
1.00
8.84
A


ATOM
219
O
ILE
A
30
103.227
66.413
43.837
1.00
20.78
A


ATOM
220
N
ALA
A
31
102.137
65.986
45.730
1.00
11.98
A


ATOM
221
CA
ALA
A
31
103.289
65.247
46.282
1.00
16.32
A


ATOM
222
CB
ALA
A
31
102.982
64.774
47.668
1.00
11.90
A


ATOM
223
C
ALA
A
31
103.613
64.026
45.381
1.00
20.08
A


ATOM
224
O
ALA
A
31
104.779
63.774
45.047
1.00
18.22
A


ATOM
225
N
VAL
A
32
102.594
63.254
45.005
1.00
16.38
A


ATOM
226
CA
VAL
A
32
102.852
62.078
44.161
1.00
18.95
A


ATOM
227
CB
VAL
A
32
101.597
61.176
44.042
1.00
22.56
A


ATOM
228
CG1
VAL
A
32
101.803
60.122
42.937
1.00
22.34
A


ATOM
229
CG2
VAL
A
32
101.348
60.477
45.364
1.00
13.55
A


ATOM
230
C
VAL
A
32
103.328
62.569
42.778
1.00
22.75
A


ATOM
231
O
VAL
A
32
104.310
62.061
42.211
1.00
22.15
A


ATOM
232
N
HIS
A
33
102.662
63.581
42.234
1.00
17.13
A


ATOM
233
CA
HIS
A
33
103.113
64.112
40.945
1.00
15.67
A


ATOM
234
CB
HIS
A
33
102.301
65.335
40.554
1.00
19.93
A


ATOM
235
CG
HIS
A
33
102.849
66.078
39.380
1.00
19.22
A


ATOM
236
CD2
HIS
A
33
103.579
67.220
39.317
1.00
25.53
A


ATOM
237
ND1
HIS
A
33
102.541
65.747
38.076
1.00
24.42
A


ATOM
238
CE1
HIS
A
33
103.042
66.661
37.262
1.00
21.19
A


ATOM
239
NE2
HIS
A
33
103.674
67.567
37.992
1.00
27.74
A


ATOM
240
C
HIS
A
33
104.580
64.549
41.036
1.00
15.68
A


ATOM
241
O
HIS
A
33
105.364
64.348
40.108
1.00
20.03
A


ATOM
242
N
TYR
A
34
104.944
65.179
42.144
1.00
16.97
A


ATOM
243
CA
TYR
A
34
106.317
65.646
42.304
1.00
20.05
A


ATOM
244
CB
TYR
A
34
106.420
66.500
43.571
1.00
19.93
A


ATOM
245
CG
TYR
A
34
107.826
66.882
43.969
1.00
14.48
A


ATOM
246
CD1
TYR
A
34
108.374
68.143
43.629
1.00
19.63
A


ATOM
247
CE1
TYR
A
34
109.672
68.522
44.095
1.00
15.16
A


ATOM
248
CD2
TYR
A
34
108.597
66.021
44.746
1.00
21.63
A


ATOM
249
CE2
TYR
A
34
109.889
66.380
45.183
1.00
17.33
A


ATOM
250
CZ
TYR
A
34
110.419
67.633
44.861
1.00
21.32
A


ATOM
251
OH
TYR
A
34
111.695
67.974
45.318
1.00
20.25
A


ATOM
252
C
TYR
A
34
107.326
64.471
42.392
1.00
21.84
A


ATOM
253
O
TYR
A
34
108.389
64.514
41.770
1.00
20.07
A


ATOM
254
N
LEU
A
35
107.010
63.454
43.197
1.00
19.69
A


ATOM
255
CA
LEU
A
35
107.924
62.333
43.345
1.00
19.40
A


ATOM
256
CB
LEU
A
35
107.451
61.435
44.478
1.00
17.56
A


ATOM
257
CG
LEU
A
35
107.627
62.047
45.884
1.00
16.05
A


ATOM
258
CD1
LEU
A
35
106.927
61.165
46.915
1.00
19.26
A


ATOM
259
CD2
LEU
A
35
109.123
62.096
46.253
1.00
15.28
A


ATOM
260
C
LEU
A
35
108.066
61.564
42.015
1.00
23.12
A


ATOM
261
O
LEU
A
35
109.187
61.215
41.608
1.00
17.85
A


ATOM
262
N
ASP
A
36
106.954
61.305
41.329
1.00
16.70
A


ATOM
263
CA
ASP
A
36
107.009
60.614
40.024
1.00
21.87
A


ATOM
264
CB
ASP
A
36
105.620
60.458
39.375
1.00
23.42
A


ATOM
265
CG
ASP
A
36
104.722
59.442
40.071
1.00
19.96
A


ATOM
266
OD1
ASP
A
36
105.160
58.736
41.003
1.00
23.17
A


ATOM
267
OD2
ASP
A
36
103.556
59.366
39.646
1.00
26.39
A


ATOM
268
C
ASP
A
36
107.830
61.430
39.021
1.00
26.32
A


ATOM
269
O
ASP
A
36
108.750
60.927
38.360
1.00
22.51
A


ATOM
270
N
GLU
A
37
107.505
62.709
38.909
1.00
19.41
A


ATOM
271
CA
GLU
A
37
108.180
63.550
37.919
1.00
23.12
A


ATOM
272
CB
GLU
A
37
107.476
64.916
37.874
1.00
31.20
A


ATOM
273
CG
GLU
A
37
107.620
65.640
36.601
1.00
42.72
A


ATOM
274
CD
GLU
A
37
106.461
65.412
35.676
1.00
49.79
A


ATOM
275
OE1
GLU
A
37
106.263
64.269
35.210
1.00
51.12
A


ATOM
276
OE2
GLU
A
37
105.728
66.387
35.415
1.00
58.13
A


ATOM
277
C
GLU
A
37
109.679
63.735
38.197
1.00
28.37
A


ATOM
278
O
GLU
A
37
110.506
63.788
37.269
1.00
19.17
A


ATOM
279
N
THR
A
38
110.044
63.821
39.469
1.00
19.70
A


ATOM
280
CA
THR
A
38
111.446
64.046
39.777
1.00
18.16
A


ATOM
281
CB
THR
A
38
111.615
64.951
41.051
1.00
24.32
A


ATOM
282
OG1
THR
A
38
111.062
64.292
42.206
1.00
17.55
A


ATOM
283
CG2
THR
A
38
110.872
66.295
40.874
1.00
17.35
A


ATOM
284
C
THR
A
38
112.173
62.725
39.992
1.00
23.12
A


ATOM
285
O
THR
A
38
113.384
62.708
40.176
1.00
20.01
A


ATOM
286
N
GLU
A
39
111.423
61.626
39.973
1.00
19.41
A


ATOM
287
CA
GLU
A
39
111.997
60.298
40.178
1.00
24.05
A


ATOM
288
CB
GLU
A
39
112.918
59.965
38.997
1.00
26.82
A


ATOM
289
CG
GLU
A
39
112.089
59.751
37.704
1.00
27.56
A


ATOM
290
CD
GLU
A
39
112.915
59.531
36.414
1.00
40.15
A


ATOM
291
OE1
GLU
A
39
113.910
58.776
36.430
1.00
32.93
A


ATOM
292
OE2
GLU
A
39
112.555
60.113
35.358
1.00
42.52
A


ATOM
293
C
GLU
A
39
112.740
60.228
41.519
1.00
25.56
A


ATOM
294
O
GLU
A
39
113.898
59.818
41.597
1.00
23.18
A


ATOM
295
N
GLN
A
40
112.059
60.614
42.601
1.00
24.46
A


ATOM
296
CA
GLN
A
40
112.707
60.592
43.899
1.00
18.16
A


ATOM
297
CB
GLN
A
40
112.682
62.002
44.489
1.00
24.56
A


ATOM
298
CG
GLN
A
40
113.517
63.012
43.708
1.00
10.67
A


ATOM
299
CD
GLN
A
40
113.705
64.283
44.507
1.00
19.34
A


ATOM
300
OE1
GLN
A
40
114.594
64.350
45.334
1.00
17.51
A


ATOM
301
NE2
GLN
A
40
112.845
65.298
44.275
1.00
17.15
A


ATOM
302
C
GLN
A
40
112.118
59.603
44.894
1.00
19.24
A


ATOM
303
O
GLN
A
40
112.350
59.725
46.100
1.00
23.09
A


ATOM
304
N
TRP
A
41
111.345
58.635
44.403
1.00
18.32
A


ATOM
305
CA
TRP
A
41
110.700
57.670
45.311
1.00
18.31
A


ATOM
306
CB
TRP
A
41
109.808
56.715
44.511
1.00
18.08
A


ATOM
307
CG
TRP
A
41
108.504
57.334
44.147
1.00
16.18
A


ATOM
308
CD2
TRP
A
41
107.382
57.574
45.024
1.00
18.90
A


ATOM
309
CE2
TRP
A
41
106.376
58.204
44.257
1.00
16.03
A


ATOM
310
CE3
TRP
A
41
107.134
57.322
46.379
1.00
16.13
A


ATOM
311
CD1
TRP
A
41
108.139
57.816
42.926
1.00
20.37
A


ATOM
312
NE1
TRP
A
41
106.860
58.344
42.977
1.00
19.88
A


ATOM
313
CZ2
TRP
A
41
105.136
58.583
44.800
1.00
15.32
A


ATOM
314
CZ3
TRP
A
41
105.893
57.703
46.929
1.00
19.86
A


ATOM
315
CH2
TRP
A
41
104.908
58.330
46.127
1.00
17.50
A


ATOM
316
C
TRP
A
41
111.717
56.895
46.152
1.00
23.10
A


ATOM
317
O
TRP
A
41
111.457
56.555
47.312
1.00
24.58
A


ATOM
318
N
GLU
A
42
112.886
56.633
45.587
1.00
22.30
A


ATOM
319
CA
GLU
A
42
113.937
55.916
46.322
1.00
30.40
A


ATOM
320
CB
GLU
A
42
115.163
55.750
45.427
1.00
36.58
A


ATOM
321
CG
GLU
A
42
116.448
55.477
46.147
1.00
53.35
A


ATOM
322
CD
GLU
A
42
116.751
54.016
46.210
1.00
62.20
A


ATOM
323
OE1
GLU
A
42
117.946
53.652
46.164
1.00
67.07
A


ATOM
324
OE2
GLU
A
42
115.790
53.227
46.306
1.00
71.58
A


ATOM
325
C
GLU
A
42
114.292
56.689
47.595
1.00
26.77
A


ATOM
326
O
GLU
A
42
114.483
56.112
48.658
1.00
25.14
A


ATOM
327
N
LYS
A
43
114.352
58.005
47.515
1.00
24.54
A


ATOM
328
CA
LYS
A
43
114.639
58.771
48.717
1.00
25.18
A


ATOM
329
CB
LYS
A
43
115.106
60.173
48.344
1.00
33.24
A


ATOM
330
CG
LYS
A
43
116.398
60.152
47.566
1.00
36.94
A


ATOM
331
CD
LYS
A
43
117.068
61.498
47.549
1.00
39.52
A


ATOM
332
CE
LYS
A
43
116.841
62.204
46.242
1.00
39.10
A


ATOM
333
NZ
LYS
A
43
117.597
63.486
46.197
1.00
37.63
A


ATOM
334
C
LYS
A
43
113.437
58.885
49.640
1.00
30.60
A


ATOM
335
O
LYS
A
43
113.583
59.064
50.854
1.00
28.31
A


ATOM
336
N
PHE
A
44
112.232
58.754
49.089
1.00
23.22
A


ATOM
337
CA
PHE
A
44
111.061
58.940
49.924
1.00
22.11
A


ATOM
338
CB
PHE
A
44
109.995
59.654
49.078
1.00
19.24
A


ATOM
339
CG
PHE
A
44
108.732
59.989
49.809
1.00
21.27
A


ATOM
340
CD1
PHE
A
44
108.627
61.162
50.555
1.00
23.33
A


ATOM
341
CD2
PHE
A
44
107.624
59.154
49.710
1.00
22.69
A


ATOM
342
CE1
PHE
A
44
107.424
61.514
51.193
1.00
22.52
A


ATOM
343
CE2
PHE
A
44
106.405
59.486
50.344
1.00
27.02
A


ATOM
344
CZ
PHE
A
44
106.306
60.674
51.083
1.00
27.40
A


ATOM
345
C
PHE
A
44
110.533
57.628
50.510
1.00
24.45
A


ATOM
346
O
PHE
A
44
110.059
57.605
51.647
1.00
18.55
A


ATOM
347
N
GLY
A
45
110.636
56.550
49.730
1.00
20.97
A


ATOM
348
CA
GLY
A
45
110.142
55.236
50.131
1.00
20.25
A


ATOM
349
C
GLY
A
45
109.064
54.826
49.136
1.00
23.29
A


ATOM
350
O
GLY
A
45
107.871
55.185
49.279
1.00
17.57
A


ATOM
351
N
LEU
A
46
109.465
54.095
48.104
1.00
20.22
A


ATOM
352
CA
LEU
A
46
108.525
53.666
47.089
1.00
18.57
A


ATOM
353
CB
LEU
A
46
109.243
52.881
45.977
1.00
27.58
A


ATOM
354
CG
LEU
A
46
108.444
52.315
44.770
1.00
35.06
A


ATOM
355
CD1
LEU
A
46
107.661
53.396
44.022
1.00
27.92
A


ATOM
356
CD2
LEU
A
46
109.443
51.614
43.809
1.00
32.82
A


ATOM
357
C
LEU
A
46
107.322
52.884
47.611
1.00
23.74
A


ATOM
358
O
LEU
A
46
106.255
52.927
46.995
1.00
19.46
A


ATOM
359
N
GLU
A
47
107.450
52.190
48.738
1.00
19.11
A


ATOM
360
CA
GLU
A
47
106.292
51.453
49.220
1.00
29.84
A


ATOM
361
CB
GLU
A
47
106.629
50.448
50.332
1.00
30.68
A


ATOM
362
CG
GLU
A
47
107.600
50.871
51.370
1.00
45.90
A


ATOM
363
CD
GLU
A
47
107.135
52.019
52.226
1.00
52.96
A


ATOM
364
OE1
GLU
A
47
105.927
52.017
52.597
1.00
60.58
A


ATOM
365
OE2
GLU
A
47
107.991
52.901
52.541
1.00
47.47
A


ATOM
366
C
GLU
A
47
105.151
52.350
49.678
1.00
27.56
A


ATOM
367
O
GLU
A
47
104.061
51.855
49.854
1.00
24.22
A


ATOM
368
N
LYS
A
48
105.403
53.655
49.861
1.00
26.01
A


ATOM
369
CA
LYS
A
48
104.340
54.594
50.262
1.00
20.77
A


ATOM
370
CB
LYS
A
48
104.924
55.863
50.888
1.00
20.15
A


ATOM
371
CG
LYS
A
48
105.694
55.636
52.167
1.00
19.21
A


ATOM
372
CD
LYS
A
48
106.344
56.939
52.656
1.00
20.50
A


ATOM
373
CE
LYS
A
48
107.190
56.673
53.891
1.00
30.14
A


ATOM
374
NZ
LYS
A
48
108.457
55.962
53.556
1.00
23.91
A


ATOM
375
C
LYS
A
48
103.512
55.013
49.059
1.00
19.66
A


ATOM
376
O
LYS
A
48
102.415
55.567
49.217
1.00
23.92
A


ATOM
377
N
ARG
A
49
104.005
54.750
47.850
1.00
17.52
A


ATOM
378
CA
ARG
A
49
103.257
55.178
46.655
1.00
21.83
A


ATOM
379
CB
ARG
A
49
104.048
54.879
45.360
1.00
16.40
A


ATOM
380
CG
ARG
A
49
103.443
55.612
44.111
1.00
14.73
A


ATOM
381
CD
ARG
A
49
104.410
55.661
42.916
1.00
21.52
A


ATOM
382
NE
ARG
A
49
103.924
56.478
41.815
1.00
16.66
A


ATOM
383
CZ
ARG
A
49
103.062
56.056
40.889
1.00
31.21
A


ATOM
384
NH1
ARG
A
49
102.592
54.803
40.922
1.00
20.87
A


ATOM
385
NH2
ARG
A
49
102.657
56.894
39.938
1.00
21.02
A


ATOM
386
C
ARG
A
49
101.826
54.612
46.536
1.00
24.68
A


ATOM
387
O
ARG
A
49
100.872
55.334
46.224
1.00
20.52
A


ATOM
388
N
GLN
A
50
101.681
53.313
46.785
1.00
31.61
A


ATOM
389
CA
GLN
A
50
100.398
52.629
46.691
1.00
24.84
A


ATOM
390
CB
GLN
A
50
100.531
51.187
47.168
1.00
33.17
A


ATOM
391
CG
GLN
A
50
100.197
50.169
46.142
1.00
46.59
A


ATOM
392
CD
GLN
A
50
98.794
50.305
45.608
1.00
42.68
A


ATOM
393
OE1
GLN
A
50
97.825
50.200
46.346
1.00
40.26
A


ATOM
394
NE2
GLN
A
50
98.684
50.542
44.308
1.00
47.02
A


ATOM
395
C
GLN
A
50
99.381
53.289
47.577
1.00
27.71
A


ATOM
396
O
GLN
A
50
98.257
53.562
47.157
1.00
18.05
A


ATOM
397
N
GLY
A
51
99.795
53.504
48.822
1.00
19.41
A


ATOM
398
CA
GLY
A
51
98.944
54.124
49.820
1.00
24.54
A


ATOM
399
C
GLY
A
51
98.517
55.489
49.329
1.00
27.23
A


ATOM
400
O
GLY
A
51
97.347
55.861
49.470
1.00
27.21
A


ATOM
401
N
ALA
A
52
99.444
56.233
48.723
1.00
21.82
A


ATOM
402
CA
ALA
A
52
99.089
57.561
48.220
1.00
21.64
A


ATOM
403
CB
ALA
A
52
100.343
58.319
47.777
1.00
20.17
A


ATOM
404
C
ALA
A
52
98.117
57.446
47.065
1.00
18.79
A


ATOM
405
O
ALA
A
52
97.209
58.265
46.947
1.00
21.57
A


ATOM
406
N
LEU
A
53
98.296
56.436
46.206
1.00
16.94
A


ATOM
407
CA
LEU
A
53
97.379
56.279
45.079
1.00
20.49
A


ATOM
408
CB
LEU
A
53
97.816
55.148
44.151
1.00
22.88
A


ATOM
409
CG
LEU
A
53
99.197
55.310
43.509
1.00
27.56
A


ATOM
410
CD1
LEU
A
53
99.526
54.081
42.643
1.00
28.51
A


ATOM
411
CD2
LEU
A
53
99.207
56.557
42.665
1.00
19.40
A


ATOM
412
C
LEU
A
53
95.966
56.027
45.571
1.00
22.20
A


ATOM
413
O
LEU
A
53
95.014
56.559
44.996
1.00
22.74
A


ATOM
414
N
GLU
A
54
95.838
55.241
46.643
1.00
20.38
A


ATOM
415
CA
GLU
A
54
94.542
54.937
47.247
1.00
22.21
A


ATOM
416
CB
GLU
A
54
94.705
53.920
48.404
1.00
22.94
A


ATOM
417
CG
GLU
A
54
95.023
52.489
47.951
1.00
41.26
A


ATOM
418
CD
GLU
A
54
95.587
51.579
49.071
1.00
48.52
A


ATOM
419
OE1
GLU
A
54
95.476
51.903
50.286
1.00
50.15
A


ATOM
420
OE2
GLU
A
54
96.141
50.514
48.717
1.00
50.86
A


ATOM
421
C
GLU
A
54
93.870
56.214
47.801
1.00
24.94
A


ATOM
422
O
GLU
A
54
92.644
56.351
47.710
1.00
21.97
A


ATOM
423
N
LEU
A
55
94.656
57.126
48.392
1.00
18.88
A


ATOM
424
CA
LEU
A
55
94.085
58.351
48.948
1.00
21.65
A


ATOM
425
CB
LEU
A
55
95.099
59.091
49.809
1.00
17.30
A


ATOM
426
CG
LEU
A
55
95.464
58.303
51.072
1.00
26.70
A


ATOM
427
CD1
LEU
A
55
96.576
59.072
51.841
1.00
25.33
A


ATOM
428
CD2
LEU
A
55
94.196
58.153
51.981
1.00
20.15
A


ATOM
429
C
LEU
A
55
93.646
59.229
47.802
1.00
18.82
A


ATOM
430
O
LEU
A
55
92.613
59.897
47.902
1.00
16.55
A


ATOM
431
N
ILE
A
56
94.413
59.218
46.712
1.00
15.84
A


ATOM
432
CA
ILE
A
56
94.065
60.024
45.513
1.00
16.74
A


ATOM
433
CB
ILE
A
56
95.151
59.930
44.390
1.00
21.15
A


ATOM
434
CG2
ILE
A
56
94.710
60.691
43.120
1.00
25.29
A


ATOM
435
CG1
ILE
A
56
96.461
60.644
44.806
1.00
20.28
A


ATOM
436
CD1
ILE
A
56
96.234
61.948
45.382
1.00
40.37
A


ATOM
437
C
ILE
A
56
92.723
59.508
44.945
1.00
25.52
A


ATOM
438
O
ILE
A
56
91.816
60.297
44.615
1.00
18.85
A


ATOM
439
N
LYS
A
57
92.589
58.183
44.822
1.00
20.21
A


ATOM
440
CA
LYS
A
57
91.354
57.595
44.308
1.00
19.21
A


ATOM
441
CB
LYS
A
57
91.496
56.088
44.221
1.00
26.24
A


ATOM
442
CG
LYS
A
57
90.244
55.363
43.763
1.00
37.40
A


ATOM
443
CD
LYS
A
57
90.531
53.831
43.577
1.00
39.50
A


ATOM
444
CE
LYS
A
57
89.290
53.088
43.091
1.00
45.08
A


ATOM
445
NZ
LYS
A
57
89.516
51.637
42.936
1.00
50.42
A


ATOM
446
C
LYS
A
57
90.192
57.952
45.242
1.00
23.30
A


ATOM
447
O
LYS
A
57
89.082
58.273
44.789
1.00
20.92
A


ATOM
448
N
LYS
A
58
90.445
57.902
46.550
1.00
16.98
A


ATOM
449
CA
LYS
A
58
89.421
58.260
47.513
1.00
17.12
A


ATOM
450
CB
LYS
A
58
89.971
58.041
48.937
1.00
18.85
A


ATOM
451
CG
LYS
A
58
88.973
58.307
50.047
1.00
25.94
A


ATOM
452
CD
LYS
A
58
89.491
57.947
51.437
1.00
35.63
A


ATOM
453
CE
LYS
A
58
88.639
58.677
52.501
1.00
42.54
A


ATOM
454
NZ
LYS
A
58
88.728
58.038
53.858
1.00
50.94
A


ATOM
455
C
LYS
A
58
89.011
59.749
47.300
1.00
25.76
A


ATOM
456
O
LYS
A
58
87.808
60.095
47.288
1.00
22.91
A


ATOM
457
N
GLY
A
59
89.999
60.629
47.099
1.00
21.23
A


ATOM
458
CA
GLY
A
59
89.691
62.041
46.894
1.00
23.41
A


ATOM
459
C
GLY
A
59
88.819
62.247
45.664
1.00
25.51
A


ATOM
460
O
GLY
A
59
87.820
62.978
45.683
1.00
21.04
A


ATOM
461
N
TYR
A
60
89.213
61.576
44.588
1.00
21.05
A


ATOM
462
CA
TYR
A
60
88.517
61.642
43.312
1.00
23.66
A


ATOM
463
CB
TYR
A
60
89.244
60.776
42.296
1.00
13.71
A


ATOM
464
CG
TYR
A
60
88.456
60.499
41.039
1.00
25.98
A


ATOM
465
CD1
TYR
A
60
88.276
61.495
40.082
1.00
22.56
A


ATOM
466
CE1
TYR
A
60
87.570
61.250
38.915
1.00
26.16
A


ATOM
467
CD2
TYR
A
60
87.893
59.223
40.792
1.00
27.02
A


ATOM
468
CE2
TYR
A
60
87.180
58.966
39.610
1.00
23.07
A


ATOM
469
CZ
TYR
A
60
87.027
59.994
38.677
1.00
27.86
A


ATOM
470
OH
TYR
A
60
86.347
59.798
37.490
1.00
19.73
A


ATOM
471
C
TYR
A
60
87.089
61.131
43.448
1.00
23.97
A


ATOM
472
O
TYR
A
60
86.129
61.776
43.024
1.00
16.61
A


ATOM
473
N
THR
A
61
86.947
59.954
44.029
1.00
22.77
A


ATOM
474
CA
THR
A
61
85.615
59.365
44.185
1.00
22.30
A


ATOM
475
CB
THR
A
61
85.739
58.003
44.849
1.00
30.49
A


ATOM
476
OG1
THR
A
61
86.448
57.145
43.960
1.00
31.94
A


ATOM
477
CG2
THR
A
61
84.371
57.401
45.164
1.00
42.36
A


ATOM
478
C
THR
A
61
84.724
60.246
45.008
1.00
23.97
A


ATOM
479
O
THR
A
61
83.554
60.480
44.668
1.00
22.40
A


ATOM
480
N
GLN
A
62
85.267
60.743
46.108
1.00
20.42
A


ATOM
481
CA
GLN
A
62
84.476
61.619
46.958
1.00
23.93
A


ATOM
482
CB
GLN
A
62
85.255
61.966
48.242
1.00
24.47
A


ATOM
483
CG
GLN
A
62
85.293
60.776
49.207
1.00
26.29
A


ATOM
484
CD
GLN
A
62
86.265
60.940
50.385
1.00
27.78
A


ATOM
485
OE1
GLN
A
62
86.263
60.126
51.289
1.00
27.57
A


ATOM
486
NE2
GLN
A
62
87.098
61.980
50.362
1.00
22.36
A


ATOM
487
C
GLN
A
62
84.037
62.885
46.215
1.00
19.82
A


ATOM
488
O
GLN
A
62
82.903
63.334
46.374
1.00
21.86
A


ATOM
489
N
GLN
A
63
84.910
63.441
45.382
1.00
20.60
A


ATOM
490
CA
GLN
A
63
84.569
64.669
44.667
1.00
23.75
A


ATOM
491
CB
GLN
A
63
85.772
65.168
43.862
1.00
17.42
A


ATOM
492
CG
GLN
A
63
85.669
66.602
43.360
1.00
15.84
A


ATOM
493
CD
GLN
A
63
85.470
67.642
44.449
1.00
18.31
A


ATOM
494
OE1
GLN
A
63
85.851
67.455
45.610
1.00
15.34
A


ATOM
495
NE2
GLN
A
63
84.900
68.779
44.062
1.00
17.92
A


ATOM
496
C
GLN
A
63
83.371
64.460
43.721
1.00
27.22
A


ATOM
497
O
GLN
A
63
82.590
65.388
43.471
1.00
16.84
A


ATOM
498
N
LEU
A
64
83.223
63.249
43.201
1.00
18.15
A


ATOM
499
CA
LEU
A
64
82.113
62.984
42.289
1.00
20.34
A


ATOM
500
CB
LEU
A
64
82.194
61.562
41.738
1.00
20.67
A


ATOM
501
CG
LEU
A
64
83.460
61.266
40.904
1.00
29.97
A


ATOM
502
CD1
LEU
A
64
83.341
59.848
40.328
1.00
28.53
A


ATOM
503
CD2
LEU
A
64
83.645
62.308
39.769
1.00
25.04
A


ATOM
504
C
LEU
A
64
80.781
63.214
42.971
1.00
20.30
A


ATOM
505
O
LEU
A
64
79.818
63.568
42.314
1.00
23.21
A


ATOM
506
N
ALA
A
65
80.708
63.015
44.288
1.00
15.07
A


ATOM
507
CA
ALA
A
65
79.459
63.267
44.975
1.00
19.06
A


ATOM
508
CB
ALA
A
65
79.595
62.955
46.447
1.00
19.25
A


ATOM
509
C
ALA
A
65
79.010
64.717
44.790
1.00
19.22
A


ATOM
510
O
ALA
A
65
77.864
65.042
45.087
1.00
24.93
A


ATOM
511
N
PHE
A
66
79.895
65.592
44.314
1.00
22.94
A


ATOM
512
CA
PHE
A
66
79.533
67.015
44.134
1.00
22.02
A


ATOM
513
CB
PHE
A
66
80.481
67.908
44.943
1.00
21.43
A


ATOM
514
CG
PHE
A
66
80.548
67.511
46.386
1.00
24.76
A


ATOM
515
CD1
PHE
A
66
79.542
67.914
47.287
1.00
21.43
A


ATOM
516
CD2
PHE
A
66
81.573
66.657
46.840
1.00
20.02
A


ATOM
517
CE1
PHE
A
66
79.555
67.467
48.605
1.00
22.96
A


ATOM
518
CE2
PHE
A
66
81.594
66.203
48.162
1.00
18.70
A


ATOM
519
CZ
PHE
A
66
80.584
66.611
49.049
1.00
21.49
A


ATOM
520
C
PHE
A
66
79.511
67.461
42.693
1.00
17.64
A


ATOM
521
O
PHE
A
66
79.405
68.673
42.389
1.00
18.14
A


ATOM
522
N
ARG
A
67
79.606
66.481
41.796
1.00
21.16
A


ATOM
523
CA
ARG
A
67
79.541
66.745
40.354
1.00
23.23
A


ATOM
524
CB
ARG
A
67
79.971
65.496
39.598
1.00
18.11
A


ATOM
525
CG
ARG
A
67
79.896
65.665
38.107
1.00
28.17
A


ATOM
526
CD
ARG
A
67
80.315
64.386
37.363
1.00
25.25
A


ATOM
527
NE
ARG
A
67
80.344
64.619
35.923
1.00
25.71
A


ATOM
528
CZ
ARG
A
67
80.004
63.715
35.012
1.00
26.53
A


ATOM
529
NH1
ARG
A
67
79.601
62.496
35.388
1.00
23.05
A


ATOM
530
NH2
ARG
A
67
80.089
64.028
33.724
1.00
16.97
A


ATOM
531
C
ARG
A
67
78.055
67.034
40.044
1.00
25.46
A


ATOM
532
O
ARG
A
67
77.223
66.244
40.427
1.00
18.92
A


ATOM
533
N
GLN
A
68
77.716
68.150
39.395
1.00
21.57
A


ATOM
534
CA
GLN
A
68
76.305
68.440
39.084
1.00
20.68
A


ATOM
535
CB
GLN
A
68
76.073
69.964
39.186
1.00
23.31
A


ATOM
536
CG
GLN
A
68
76.532
70.448
40.570
1.00
17.98
A


ATOM
537
CD
GLN
A
68
75.990
71.790
40.937
1.00
25.72
A


ATOM
538
OE1
GLN
A
68
75.464
72.528
40.084
1.00
23.05
A


ATOM
539
NE2
GLN
A
68
76.132
72.140
42.206
1.00
15.43
A


ATOM
540
C
GLN
A
68
75.871
67.887
37.722
1.00
16.68
A


ATOM
541
O
GLN
A
68
76.704
67.367
36.998
1.00
19.76
A


ATOM
542
N
PRO
A
69
74.563
67.945
37.368
1.00
22.91
A


ATOM
543
CD
PRO
A
69
73.385
68.286
38.198
1.00
21.74
A


ATOM
544
CA
PRO
A
69
74.144
67.408
36.046
1.00
20.68
A


ATOM
545
CB
PRO
A
69
72.628
67.698
35.995
1.00
17.83
A


ATOM
546
CG
PRO
A
69
72.208
67.532
37.454
1.00
16.18
A


ATOM
547
C
PRO
A
69
74.886
68.078
34.892
1.00
23.73
A


ATOM
548
O
PRO
A
69
75.152
67.467
33.875
1.00
23.28
A


ATOM
549
N
SER
A
70
75.246
69.340
35.086
1.00
24.11
A


ATOM
550
CA
SER
A
70
75.975
70.114
34.092
1.00
23.11
A


ATOM
551
CB
SER
A
70
76.000
71.580
34.528
1.00
20.68
A


ATOM
552
OG
SER
A
70
76.662
71.709
35.793
1.00
27.57
A


ATOM
553
C
SER
A
70
77.436
69.654
33.942
1.00
27.84
A


ATOM
554
O
SER
A
70
78.127
70.080
33.021
1.00
24.67
A


ATOM
555
N
SER
A
71
77.885
68.805
34.868
1.00
19.80
A


ATOM
556
CA
SER
A
71
79.288
68.333
34.971
1.00
20.11
A


ATOM
557
CB
SER
A
71
79.953
68.052
33.613
1.00
22.15
A


ATOM
558
OG
SER
A
71
79.498
66.853
33.011
1.00
21.54
A


ATOM
559
C
SER
A
71
80.131
69.401
35.702
1.00
20.52
A


ATOM
560
O
SER
A
71
81.329
69.262
35.782
1.00
19.50
A


ATOM
561
N
ALA
A
72
79.522
70.477
36.199
1.00
19.13
A


ATOM
562
CA
ALA
A
72
80.273
71.479
36.973
1.00
21.30
A


ATOM
563
CB
ALA
A
72
79.665
72.897
36.823
1.00
16.76
A


ATOM
564
C
ALA
A
72
80.250
71.064
38.447
1.00
24.51
A


ATOM
565
O
ALA
A
72
79.540
70.122
38.840
1.00
20.95
A


ATOM
566
N
PHE
A
73
81.026
71.773
39.266
1.00
22.89
A


ATOM
567
CA
PHE
A
73
81.113
71.484
40.695
1.00
19.84
A


ATOM
568
CB
PHE
A
73
82.541
70.958
41.056
1.00
16.24
A


ATOM
569
CG
PHE
A
73
82.890
69.625
40.409
1.00
17.75
A


ATOM
570
CD1
PHE
A
73
83.265
69.554
39.067
1.00
14.21
A


ATOM
571
CD2
PHE
A
73
82.791
68.439
41.140
1.00
21.80
A


ATOM
572
CE1
PHE
A
73
83.527
68.310
38.447
1.00
18.43
A


ATOM
573
CE2
PHE
A
73
83.048
67.196
40.546
1.00
20.67
A


ATOM
574
CZ
PHE
A
73
83.418
67.115
39.190
1.00
17.64
A


ATOM
575
C
PHE
A
73
80.846
72.708
41.561
1.00
15.55
A


ATOM
576
O
PHE
A
73
81.079
73.846
41.134
1.00
17.42
A


ATOM
577
N
ALA
A
74
80.392
72.437
42.787
1.00
18.86
A


ATOM
578
CA
ALA
A
74
80.196
73.440
43.842
1.00
21.20
A


ATOM
579
CB
ALA
A
74
78.774
74.076
43.782
1.00
14.22
A


ATOM
580
C
ALA
A
74
80.381
72.705
45.171
1.00
19.11
A


ATOM
581
O
ALA
A
74
80.364
71.471
45.211
1.00
23.95
A


ATOM
582
N
ALA
A
75
80.579
73.456
46.259
1.00
17.13
A


ATOM
583
CA
ALA
A
75
80.738
72.883
47.584
1.00
21.11
A


ATOM
584
CB
ALA
A
75
80.813
73.959
48.592
1.00
12.54
A


ATOM
585
C
ALA
A
75
79.600
71.944
47.961
1.00
19.04
A


ATOM
586
O
ALA
A
75
79.821
70.901
48.589
1.00
18.22
A


ATOM
587
N
PHE
A
76
78.386
72.317
47.591
1.00
20.53
A


ATOM
588
CA
PHE
A
76
77.202
71.519
47.920
1.00
15.82
A


ATOM
589
CB
PHE
A
76
76.420
72.195
49.053
1.00
17.12
A


ATOM
590
CG
PHE
A
76
77.249
72.514
50.258
1.00
26.60
A


ATOM
591
CD1
PHE
A
76
77.723
71.505
51.098
1.00
27.70
A


ATOM
592
CD2
PHE
A
76
77.576
73.824
50.551
1.00
22.87
A


ATOM
593
CE1
PHE
A
76
78.512
71.807
52.212
1.00
22.16
A


ATOM
594
CE2
PHE
A
76
78.367
74.125
51.667
1.00
28.08
A


ATOM
595
CZ
PHE
A
76
78.832
73.124
52.490
1.00
23.72
A


ATOM
596
C
PHE
A
76
76.343
71.444
46.654
1.00
23.48
A


ATOM
597
O
PHE
A
76
76.308
72.404
45.866
1.00
15.81
A


ATOM
598
N
VAL
A
77
75.639
70.327
46.465
1.00
19.15
A


ATOM
599
CA
VAL
A
77
74.845
70.141
45.255
1.00
23.84
A


ATOM
600
CB
VAL
A
77
74.279
68.688
45.144
1.00
24.38
A


ATOM
601
CG1
VAL
A
77
75.456
67.682
44.930
1.00
20.82
A


ATOM
602
CG2
VAL
A
77
73.465
68.349
46.390
1.00
19.64
A


ATOM
603
C
VAL
A
77
73.701
71.122
45.082
1.00
20.75
A


ATOM
604
O
VAL
A
77
73.201
71.320
43.973
1.00
24.71
A


ATOM
605
N
LYS
A
78
73.302
71.723
46.184
1.00
15.92
A


ATOM
606
CA
LYS
A
78
72.245
72.712
46.200
1.00
28.31
A


ATOM
607
CB
LYS
A
78
71.642
72.818
47.590
1.00
31.59
A


ATOM
608
CG
LYS
A
78
70.888
71.559
47.898
1.00
52.94
A


ATOM
609
CD
LYS
A
78
69.877
71.719
48.992
1.00
60.83
A


ATOM
610
CE
LYS
A
78
68.914
70.543
48.967
1.00
61.05
A


ATOM
611
NZ
LYS
A
78
68.246
70.353
47.672
1.00
30.69
A


ATOM
612
C
LYS
A
78
72.755
74.071
45.823
1.00
26.47
A


ATOM
613
O
LYS
A
78
71.956
74.971
45.569
1.00
24.70
A


ATOM
614
N
ARG
A
79
74.080
74.223
45.818
1.00
20.39
A


ATOM
615
CA
ARG
A
79
74.723
75.499
45.514
1.00
21.78
A


ATOM
616
CB
ARG
A
79
76.013
75.592
46.349
1.00
19.49
A


ATOM
617
CG
ARG
A
79
76.859
76.847
46.120
1.00
23.95
A


ATOM
618
CD
ARG
A
79
78.098
76.897
47.058
1.00
20.40
A


ATOM
619
NE
ARG
A
79
78.758
78.204
47.005
1.00
25.41
A


ATOM
620
CZ
ARG
A
79
78.559
79.167
47.899
1.00
30.60
A


ATOM
621
NH1
ARG
A
79
77.724
78.956
48.915
1.00
29.61
A


ATOM
622
NH2
ARG
A
79
79.177
80.338
47.780
1.00
23.38
A


ATOM
623
C
ARG
A
79
75.036
75.716
44.030
1.00
21.79
A


ATOM
624
O
ARG
A
79
75.417
74.764
43.332
1.00
19.63
A


ATOM
625
N
ALA
A
80
74.877
76.967
43.553
1.00
21.57
A


ATOM
626
CA
ALA
A
80
75.180
77.331
42.159
1.00
26.40
A


ATOM
627
CB
ALA
A
80
74.960
78.868
41.923
1.00
22.62
A


ATOM
628
C
ALA
A
80
76.659
76.953
41.914
1.00
20.22
A


ATOM
629
O
ALA
A
80
77.519
77.179
42.780
1.00
23.83
A


ATOM
630
N
PRO
A
81
76.959
76.365
40.744
1.00
23.02
A


ATOM
631
CD
PRO
A
81
75.984
76.235
39.641
1.00
22.02
A


ATOM
632
CA
PRO
A
81
78.301
75.909
40.337
1.00
20.18
A


ATOM
633
CB
PRO
A
81
78.046
75.207
38.995
1.00
23.34
A


ATOM
634
CG
PRO
A
81
76.879
75.999
38.416
1.00
25.62
A


ATOM
635
C
PRO
A
81
79.370
76.990
40.238
1.00
24.53
A


ATOM
636
O
PRO
A
81
79.132
78.085
39.736
1.00
18.24
A


ATOM
637
N
SER
A
82
80.560
76.677
40.736
1.00
18.76
A


ATOM
638
CA
SER
A
82
81.648
77.630
40.669
1.00
15.88
A


ATOM
639
CB
SER
A
82
82.494
77.549
41.942
1.00
19.09
A


ATOM
640
OG
SER
A
82
83.781
78.102
41.725
1.00
15.72
A


ATOM
641
C
SER
A
82
82.529
77.411
39.437
1.00
18.93
A


ATOM
642
O
SER
A
82
82.981
76.286
39.114
1.00
17.23
A


ATOM
643
N
THR
A
83
82.762
78.494
38.725
1.00
17.20
A


ATOM
644
CA
THR
A
83
83.628
78.442
37.544
1.00
16.78
A


ATOM
645
CB
THR
A
83
83.692
79.829
36.868
1.00
19.03
A


ATOM
646
OG1
THR
A
83
82.397
80.168
36.392
1.00
20.73
A


ATOM
647
CG2
THR
A
83
84.666
79.840
35.692
1.00
17.21
A


ATOM
648
C
THR
A
83
85.060
78.069
38.027
1.00
20.11
A


ATOM
649
O
THR
A
83
85.740
77.214
37.446
1.00
15.09
A


ATOM
650
N
TRP
A
84
85.514
78.745
39.084
1.00
16.86
A


ATOM
651
CA
TRP
A
84
86.839
78.483
39.627
1.00
24.71
A


ATOM
652
CB
TRP
A
84
87.171
79.427
40.780
1.00
16.82
A


ATOM
653
CG
TRP
A
84
88.624
79.255
41.247
1.00
17.86
A


ATOM
654
CD2
TRP
A
84
89.106
78.315
42.218
1.00
16.17
A


ATOM
655
CE2
TRP
A
84
90.510
78.464
42.284
1.00
22.04
A


ATOM
656
CE3
TRP
A
84
88.486
77.366
43.035
1.00
17.61
A


ATOM
657
CD1
TRP
A
84
89.727
79.915
40.783
1.00
23.03
A


ATOM
658
NE1
TRP
A
84
90.859
79.447
41.405
1.00
16.07
A


ATOM
659
CZ2
TRP
A
84
91.308
77.686
43.141
1.00
21.59
A


ATOM
660
CZ3
TRP
A
84
89.279
76.595
43.892
1.00
21.38
A


ATOM
661
CH2
TRP
A
84
90.670
76.759
43.936
1.00
17.83
A


ATOM
662
C
TRP
A
84
87.001
77.049
40.136
1.00
22.89
A


ATOM
663
O
TRP
A
84
87.973
76.398
39.800
1.00
18.84
A


ATOM
664
N
LEU
A
85
86.065
76.561
40.949
1.00
23.94
A


ATOM
665
CA
LEU
A
85
86.175
75.193
41.495
1.00
19.54
A


ATOM
666
CB
LEU
A
85
85.011
74.891
42.475
1.00
14.42
A


ATOM
667
CG
LEU
A
85
85.115
73.580
43.258
1.00
19.45
A


ATOM
668
CD1
LEU
A
85
86.371
73.566
44.197
1.00
11.14
A


ATOM
669
CD2
LEU
A
85
83.825
73.422
44.060
1.00
18.37
A


ATOM
670
C
LEU
A
85
86.175
74.195
40.356
1.00
24.50
A


ATOM
671
O
LEU
A
85
86.994
73.257
40.330
1.00
19.38
A


ATOM
672
N
THR
A
86
85.285
74.413
39.383
1.00
16.67
A


ATOM
673
CA
THR
A
86
85.226
73.494
38.279
1.00
18.11
A


ATOM
674
CB
THR
A
86
84.068
73.818
37.357
1.00
17.96
A


ATOM
675
OG1
THR
A
86
82.871
73.696
38.127
1.00
12.56
A


ATOM
676
CG2
THR
A
86
84.007
72.851
36.202
1.00
14.63
A


ATOM
677
C
THR
A
86
86.534
73.480
37.569
1.00
18.80
A


ATOM
678
O
THR
A
86
87.057
72.395
37.283
1.00
15.97
A


ATOM
679
N
ALA
A
87
87.111
74.657
37.315
1.00
15.61
A


ATOM
680
CA
ALA
A
87
88.422
74.679
36.645
1.00
21.28
A


ATOM
681
CB
ALA
A
87
88.812
76.132
36.265
1.00
17.13
A


ATOM
682
C
ALA
A
87
89.532
74.068
37.535
1.00
21.82
A


ATOM
683
O
ALA
A
87
90.531
73.527
37.036
1.00
18.47
A


ATOM
684
N
TYR
A
88
89.400
74.203
38.845
1.00
16.96
A


ATOM
685
CA
TYR
A
88
90.439
73.637
39.708
1.00
19.62
A


ATOM
686
CB
TYR
A
88
90.324
74.155
41.140
1.00
17.51
A


ATOM
687
CG
TYR
A
88
91.533
73.749
41.989
1.00
17.09
A


ATOM
688
CD1
TYR
A
88
92.843
73.975
41.528
1.00
17.38
A


ATOM
689
CE1
TYR
A
88
93.947
73.554
42.259
1.00
18.76
A


ATOM
690
CD2
TYR
A
88
91.366
73.093
43.214
1.00
20.06
A


ATOM
691
CE2
TYR
A
88
92.475
72.663
43.971
1.00
20.12
A


ATOM
692
CZ
TYR
A
88
93.746
72.903
43.488
1.00
20.21
A


ATOM
693
OH
TYR
A
88
94.817
72.537
44.255
1.00
13.27
A


ATOM
694
C
TYR
A
88
90.318
72.119
39.706
1.00
21.43
A


ATOM
695
O
TYR
A
88
91.316
71.390
39.776
1.00
16.93
A


ATOM
696
N
VAL
A
89
89.092
71.625
39.636
1.00
19.57
A


ATOM
697
CA
VAL
A
89
88.949
70.192
39.599
1.00
19.82
A


ATOM
698
CB
VAL
A
89
87.486
69.796
39.599
1.00
18.50
A


ATOM
699
CG1
VAL
A
89
87.331
68.263
39.251
1.00
15.01
A


ATOM
700
CG2
VAL
A
89
86.918
70.101
40.995
1.00
13.83
A


ATOM
701
C
VAL
A
89
89.659
69.678
38.349
1.00
23.95
A


ATOM
702
O
VAL
A
89
90.348
68.656
38.395
1.00
18.30
A


ATOM
703
N
VAL
A
90
89.491
70.382
37.232
1.00
17.40
A


ATOM
704
CA
VAL
A
90
90.160
69.981
35.990
1.00
18.85
A


ATOM
705
CB
VAL
A
90
89.724
70.929
34.791
1.00
19.41
A


ATOM
706
CG1
VAL
A
90
90.600
70.715
33.553
1.00
13.53
A


ATOM
707
CG2
VAL
A
90
88.248
70.648
34.399
1.00
17.29
A


ATOM
708
C
VAL
A
90
91.703
70.043
36.175
1.00
19.91
A


ATOM
709
O
VAL
A
90
92.452
69.188
35.696
1.00
20.47
A


ATOM
710
N
LYS
A
91
92.181
71.075
36.858
1.00
20.83
A


ATOM
711
CA
LYS
A
91
93.614
71.240
37.046
1.00
23.83
A


ATOM
712
CB
LYS
A
91
93.861
72.579
37.741
1.00
21.45
A


ATOM
713
CG
LYS
A
91
95.232
73.277
37.527
1.00
24.31
A


ATOM
714
CD
LYS
A
91
95.603
73.978
38.817
1.00
28.86
A


ATOM
715
CE
LYS
A
91
96.264
75.359
38.681
1.00
41.38
A


ATOM
716
NZ
LYS
A
91
97.276
75.477
37.603
1.00
23.09
A


ATOM
717
C
LYS
A
91
94.176
70.090
37.904
1.00
22.83
A


ATOM
718
O
LYS
A
91
95.288
69.615
37.659
1.00
28.75
A


ATOM
719
N
VAL
A
92
93.422
69.641
38.908
1.00
16.52
A


ATOM
720
CA
VAL
A
92
93.938
68.571
39.767
1.00
15.54
A


ATOM
721
CB
VAL
A
92
93.220
68.564
41.134
1.00
14.74
A


ATOM
722
CG1
VAL
A
92
93.669
67.333
41.957
1.00
12.97
A


ATOM
723
CG2
VAL
A
92
93.531
69.882
41.912
1.00
15.57
A


ATOM
724
C
VAL
A
92
93.780
67.195
39.100
1.00
22.13
A


ATOM
725
O
VAL
A
92
94.735
66.398
39.027
1.00
19.97
A


ATOM
726
N
PHE
A
93
92.575
66.936
38.599
1.00
15.35
A


ATOM
727
CA
PHE
A
93
92.262
65.649
37.986
1.00
21.59
A


ATOM
728
CB
PHE
A
93
90.780
65.619
37.538
1.00
20.43
A


ATOM
729
CG
PHE
A
93
89.762
65.399
38.658
1.00
21.63
A


ATOM
730
CD1
PHE
A
93
90.101
65.531
40.003
1.00
22.34
A


ATOM
731
CD2
PHE
A
93
88.435
65.083
38.327
1.00
25.57
A


ATOM
732
CE1
PHE
A
93
89.146
65.347
41.015
1.00
18.59
A


ATOM
733
CE2
PHE
A
93
87.474
64.900
39.309
1.00
16.00
A


ATOM
734
CZ
PHE
A
93
87.827
65.034
40.660
1.00
18.70
A


ATOM
735
C
PHE
A
93
93.183
65.348
36.778
1.00
20.69
A


ATOM
736
O
PHE
A
93
93.572
64.201
36.582
1.00
22.65
A


ATOM
737
N
SER
A
94
93.516
66.364
35.975
1.00
19.95
A


ATOM
738
CA
SER
A
94
94.382
66.192
34.795
1.00
23.79
A


ATOM
739
CB
SER
A
94
94.563
67.526
34.040
1.00
23.74
A


ATOM
740
OG
SER
A
94
93.351
67.930
33.393
1.00
23.03
A


ATOM
741
C
SER
A
94
95.759
65.623
35.169
1.00
29.47
A


ATOM
742
O
SER
A
94
96.379
64.867
34.406
1.00
24.55
A


ATOM
743
N
LEU
A
95
96.230
65.998
36.346
1.00
25.17
A


ATOM
744
CA
LEU
A
95
97.500
65.515
36.858
1.00
29.40
A


ATOM
745
CB
LEU
A
95
97.953
66.430
37.992
1.00
32.98
A


ATOM
746
CG
LEU
A
95
99.047
67.438
37.643
1.00
41.49
A


ATOM
747
CD1
LEU
A
95
98.732
68.151
36.358
1.00
36.09
A


ATOM
748
CD2
LEU
A
95
99.244
68.378
38.800
1.00
40.51
A


ATOM
749
C
LEU
A
95
97.413
64.064
37.368
1.00
31.25
A


ATOM
750
O
LEU
A
95
98.433
63.376
37.493
1.00
24.33
A


ATOM
751
N
ALA
A
96
96.203
63.614
37.685
1.00
28.03
A


ATOM
752
CA
ALA
A
96
95.991
62.260
38.208
1.00
27.75
A


ATOM
753
CB
ALA
A
96
94.901
62.301
39.286
1.00
26.88
A


ATOM
754
C
ALA
A
96
95.629
61.204
37.157
1.00
29.48
A


ATOM
755
O
ALA
A
96
95.529
60.013
37.479
1.00
25.41
A


ATOM
756
N
VAL
A
97
95.447
61.639
35.910
1.00
25.39
A


ATOM
757
CA
VAL
A
97
95.035
60.759
34.802
1.00
29.11
A


ATOM
758
CB
VAL
A
97
95.034
61.571
33.480
1.00
28.78
A


ATOM
759
CG1
VAL
A
97
95.057
60.663
32.321
1.00
38.20
A


ATOM
760
CG2
VAL
A
97
93.748
62.496
33.422
1.00
24.13
A


ATOM
761
C
VAL
A
97
95.813
59.424
34.636
1.00
33.92
A


ATOM
762
O
VAL
A
97
95.222
58.381
34.289
1.00
31.47
A


ATOM
763
N
ASN
A
98
97.124
59.457
34.848
1.00
21.59
A


ATOM
764
CA
ASN
A
98
97.923
58.240
34.770
1.00
36.51
A


ATOM
765
CB
ASN
A
98
99.222
58.471
33.983
1.00
36.15
A


ATOM
766
CG
ASN
A
98
98.971
58.837
32.510
1.00
36.17
A


ATOM
767
OD1
ASN
A
98
98.119
58.252
31.844
1.00
38.38
A


ATOM
768
ND2
ASN
A
98
99.730
59.805
32.007
1.00
40.71
A


ATOM
769
C
ASN
A
98
98.260
57.700
36.178
1.00
37.38
A


ATOM
770
O
ASN
A
98
99.138
56.844
36.316
1.00
31.38
A


ATOM
771
N
LEU
A
99
97.562
58.205
37.205
1.00
25.80
A


ATOM
772
CA
LEU
A
99
97.763
57.760
38.592
1.00
34.00
A


ATOM
773
CB
LEU
A
99
97.747
58.936
39.580
1.00
23.10
A


ATOM
774
CG
LEU
A
99
98.867
59.966
39.407
1.00
26.85
A


ATOM
775
CD1
LEU
A
99
98.812
61.017
40.508
1.00
25.56
A


ATOM
776
CD2
LEU
A
99
100.191
59.231
39.390
1.00
29.12
A


ATOM
777
C
LEU
A
99
96.656
56.804
38.994
1.00
34.25
A


ATOM
778
O
LEU
A
99
96.911
55.782
39.629
1.00
29.94
A


ATOM
779
N
ILE
A
100
95.431
57.169
38.623
1.00
24.08
A


ATOM
780
CA
ILE
A
100
94.232
56.407
38.913
1.00
28.23
A


ATOM
781
CB
ILE
A
100
93.446
57.007
40.119
1.00
26.92
A


ATOM
782
CG2
ILE
A
100
94.300
56.986
41.388
1.00
21.39
A


ATOM
783
CG1
ILE
A
100
93.022
58.457
39.786
1.00
22.04
A


ATOM
784
CD1
ILE
A
100
91.965
59.043
40.791
1.00
21.31
A


ATOM
785
C
ILE
A
100
93.340
56.511
37.684
1.00
28.89
A


ATOM
786
O
ILE
A
100
93.676
57.218
36.738
1.00
25.36
A


ATOM
787
N
ALA
A
101
92.195
55.834
37.701
1.00
21.87
A


ATOM
788
CA
ALA
A
101
91.278
55.891
36.563
1.00
25.41
A


ATOM
789
CB
ALA
A
101
90.323
54.656
36.557
1.00
29.20
A


ATOM
790
C
ALA
A
101
90.426
57.148
36.639
1.00
27.34
A


ATOM
791
O
ALA
A
101
89.546
57.235
37.493
1.00
27.43
A


ATOM
792
N
ILE
A
102
90.676
58.121
35.774
1.00
25.35
A


ATOM
793
CA
ILE
A
102
89.837
59.308
35.794
1.00
26.53
A


ATOM
794
CB
ILE
A
102
90.666
60.596
35.788
1.00
30.43
A


ATOM
795
CG2
ILE
A
102
89.775
61.805
35.343
1.00
26.72
A


ATOM
796
CG1
ILE
A
102
91.165
60.849
37.221
1.00
33.11
A


ATOM
797
CD1
ILE
A
102
92.470
61.458
37.221
1.00
45.56
A


ATOM
798
C
ILE
A
102
88.919
59.248
34.585
1.00
22.33
A


ATOM
799
O
ILE
A
102
89.378
59.106
33.461
1.00
26.71
A


ATOM
800
N
ASP
A
103
87.621
59.318
34.836
1.00
26.27
A


ATOM
801
CA
ASP
A
103
86.616
59.227
33.776
1.00
25.84
A


ATOM
802
CB
ASP
A
103
85.231
59.056
34.425
1.00
25.81
A


ATOM
803
CG
ASP
A
103
84.114
58.863
33.403
1.00
34.26
A


ATOM
804
OD1
ASP
A
103
84.361
59.023
32.187
1.00
25.53
A


ATOM
805
OD2
ASP
A
103
82.979
58.551
33.828
1.00
39.65
A


ATOM
806
C
ASP
A
103
86.642
60.484
32.900
1.00
24.26
A


ATOM
807
O
ASP
A
103
86.385
61.577
33.381
1.00
26.29
A


ATOM
808
N
SER
A
104
86.972
60.331
31.627
1.00
24.09
A


ATOM
809
CA
SER
A
104
87.010
61.465
30.700
1.00
30.37
A


ATOM
810
CB
SER
A
104
87.490
60.999
29.333
1.00
35.62
A


ATOM
811
OG
SER
A
104
88.890
60.808
29.371
1.00
42.20
A


ATOM
812
C
SER
A
104
85.689
62.219
30.544
1.00
27.77
A


ATOM
813
O
SER
A
104
85.679
63.399
30.237
1.00
26.73
A


ATOM
814
N
GLN
A
105
84.576
61.534
30.741
1.00
26.58
A


ATOM
815
CA
GLN
A
105
83.264
62.158
30.673
1.00
31.05
A


ATOM
816
CB
GLN
A
105
82.162
61.141
30.991
1.00
33.79
A


ATOM
817
CG
GLN
A
105
81.964
60.052
29.984
1.00
45.91
A


ATOM
818
CD
GLN
A
105
81.570
60.627
28.655
1.00
53.89
A


ATOM
819
OE1
GLN
A
105
82.400
61.212
27.938
1.00
55.25
A


ATOM
820
NE2
GLN
A
105
80.286
60.499
28.320
1.00
55.15
A


ATOM
821
C
GLN
A
105
83.227
63.209
31.778
1.00
30.55
A


ATOM
822
O
GLN
A
105
82.602
64.258
31.644
1.00
26.07
A


ATOM
823
N
VAL
A
106
83.867
62.889
32.897
1.00
26.33
A


ATOM
824
CA
VAL
A
106
83.888
63.790
34.056
1.00
25.39
A


ATOM
825
CB
VAL
A
106
84.395
63.046
35.315
1.00
24.50
A


ATOM
826
CG1
VAL
A
106
84.635
64.032
36.470
1.00
21.15
A


ATOM
827
CG2
VAL
A
106
83.369
61.966
35.738
1.00
18.61
A


ATOM
828
C
VAL
A
106
84.829
64.931
33.742
1.00
22.14
A


ATOM
829
O
VAL
A
106
84.446
66.083
33.719
1.00
25.05
A


ATOM
830
N
LEU
A
107
86.076
64.589
33.498
1.00
20.26
A


ATOM
831
CA
LEU
A
107
87.076
65.582
33.195
1.00
22.29
A


ATOM
832
CB
LEU
A
107
88.416
64.907
32.888
1.00
19.77
A


ATOM
833
CG
LEU
A
107
89.520
65.924
32.603
1.00
28.38
A


ATOM
834
CD1
LEU
A
107
89.898
66.617
33.883
1.00
29.44
A


ATOM
835
CD2
LEU
A
107
90.737
65.230
32.030
1.00
37.22
A


ATOM
836
C
LEU
A
107
86.652
66.472
32.017
1.00
25.15
A


ATOM
837
O
LEU
A
107
86.572
67.690
32.153
1.00
24.72
A


ATOM
838
N
CYS
A
108
86.334
65.874
30.877
1.00
23.13
A


ATOM
839
CA
CYS
A
108
85.959
66.654
29.711
1.00
20.45
A


ATOM
840
C
CYS
A
108
84.576
67.314
29.811
1.00
27.92
A


ATOM
841
O
CYS
A
108
84.319
68.330
29.156
1.00
25.68
A


ATOM
842
CB
CYS
A
108
86.043
65.787
28.446
1.00
27.49
A


ATOM
843
SG
CYS
A
108
87.690
65.037
28.227
1.00
28.60
A


ATOM
844
N
GLY
A
109
83.684
66.748
30.613
1.00
29.41
A


ATOM
845
CA
GLY
A
109
82.370
67.355
30.765
1.00
21.87
A


ATOM
846
C
GLY
A
109
82.542
68.681
31.483
1.00
26.48
A


ATOM
847
O
GLY
A
109
81.879
69.660
31.155
1.00
24.15
A


ATOM
848
N
ALA
A
110
83.475
68.737
32.440
1.00
19.49
A


ATOM
849
CA
ALA
A
110
83.684
69.970
33.184
1.00
17.06
A


ATOM
850
CB
ALA
A
110
84.578
69.736
34.402
1.00
18.01
A


ATOM
851
C
ALA
A
110
84.318
70.995
32.258
1.00
18.22
A


ATOM
852
O
ALA
A
110
83.970
72.180
32.307
1.00
19.57
A


ATOM
853
N
VAL
A
111
85.268
70.552
31.450
1.00
21.00
A


ATOM
854
CA
VAL
A
111
85.947
71.421
30.492
1.00
19.95
A


ATOM
855
CB
VAL
A
111
87.013
70.609
29.645
1.00
26.47
A


ATOM
856
CG1
VAL
A
111
87.331
71.346
28.314
1.00
19.61
A


ATOM
857
CG2
VAL
A
111
88.307
70.414
30.463
1.00
20.68
A


ATOM
858
C
VAL
A
111
84.883
72.006
29.549
1.00
25.73
A


ATOM
859
O
VAL
A
111
84.835
73.210
29.305
1.00
24.22
A


ATOM
860
N
LYS
A
112
84.016
71.143
29.034
1.00
24.78
A


ATOM
861
CA
LYS
A
112
82.996
71.589
28.096
1.00
29.18
A


ATOM
862
CB
LYS
A
112
82.162
70.391
27.599
1.00
29.48
A


ATOM
863
CG
LYS
A
112
81.141
70.772
26.507
1.00
35.04
A


ATOM
864
CD
LYS
A
112
80.441
69.565
25.878
1.00
35.71
A


ATOM
865
CE
LYS
A
112
81.420
68.677
25.104
1.00
41.21
A


ATOM
866
NZ
LYS
A
112
80.780
67.431
24.529
1.00
43.02
A


ATOM
867
C
LYS
A
112
82.088
72.660
28.688
1.00
24.46
A


ATOM
868
O
LYS
A
112
81.776
73.643
28.024
1.00
26.79
A


ATOM
869
N
TRP
A
113
81.672
72.470
29.939
1.00
23.58
A


ATOM
870
CA
TRP
A
113
80.810
73.415
30.626
1.00
20.72
A


ATOM
871
CB
TRP
A
113
80.459
72.878
32.014
1.00
25.99
A


ATOM
872
CG
TRP
A
113
79.627
73.817
32.856
1.00
22.08
A


ATOM
873
CD2
TRP
A
113
80.101
74.659
33.909
1.00
24.19
A


ATOM
874
CE2
TRP
A
113
78.961
75.282
34.494
1.00
24.99
A


ATOM
875
CE3
TRP
A
113
81.379
74.945
34.426
1.00
19.23
A


ATOM
876
CD1
TRP
A
113
78.251
73.971
32.828
1.00
19.34
A


ATOM
877
NE1
TRP
A
113
77.856
74.845
33.820
1.00
20.42
A


ATOM
878
CZ2
TRP
A
113
79.060
76.153
35.557
1.00
22.43
A


ATOM
879
CZ3
TRP
A
113
81.484
75.808
35.491
1.00
26.60
A


ATOM
880
CH2
TRP
A
113
80.324
76.408
36.056
1.00
30.42
A


ATOM
881
C
TRP
A
113
81.475
74.775
30.793
1.00
23.72
A


ATOM
882
O
TRP
A
113
80.848
75.815
30.600
1.00
21.74
A


ATOM
883
N
LEU
A
114
82.750
74.756
31.182
1.00
23.73
A


ATOM
884
CA
LEU
A
114
83.508
75.976
31.396
1.00
19.99
A


ATOM
885
CB
LEU
A
114
84.971
75.624
31.687
1.00
16.92
A


ATOM
886
CG
LEU
A
114
85.231
75.166
33.138
1.00
17.13
A


ATOM
887
CD1
LEU
A
114
86.620
74.621
33.322
1.00
14.89
A


ATOM
888
CD2
LEU
A
114
85.050
76.365
34.046
1.00
17.43
A


ATOM
889
C
LEU
A
114
83.413
76.839
30.143
1.00
24.77
A


ATOM
890
O
LEU
A
114
83.101
78.042
30.204
1.00
20.50
A


ATOM
891
N
ILE
A
115
83.676
76.197
29.008
1.00
23.02
A


ATOM
892
CA
ILE
A
115
83.670
76.848
27.702
1.00
26.10
A


ATOM
893
CB
ILE
A
115
84.191
75.920
26.552
1.00
24.85
A


ATOM
894
CG2
ILE
A
115
84.215
76.694
25.249
1.00
27.04
A


ATOM
895
CG1
ILE
A
115
85.585
75.368
26.851
1.00
32.98
A


ATOM
896
CD1
ILE
A
115
86.479
76.372
27.492
1.00
52.20
A


ATOM
897
C
ILE
A
115
82.287
77.278
27.240
1.00
23.55
A


ATOM
898
O
ILE
A
115
82.087
78.433
26.884
1.00
21.22
A


ATOM
899
N
LEU
A
116
81.356
76.330
27.222
1.00
15.47
A


ATOM
900
CA
LEU
A
116
80.009
76.584
26.722
1.00
23.51
A


ATOM
901
CB
LEU
A
116
79.298
75.260
26.459
1.00
23.69
A


ATOM
902
CG
LEU
A
116
79.986
74.298
25.494
1.00
29.79
A


ATOM
903
CD1
LEU
A
116
78.985
73.211
25.148
1.00
24.68
A


ATOM
904
CD2
LEU
A
116
80.479
75.022
24.250
1.00
26.74
A


ATOM
905
C
LEU
A
116
79.126
77.454
27.598
1.00
22.96
A


ATOM
906
O
LEU
A
116
78.214
78.095
27.100
1.00
23.54
A


ATOM
907
N
GLU
A
117
79.386
77.479
28.902
1.00
26.63
A


ATOM
908
CA
GLU
A
117
78.567
78.272
29.813
1.00
24.27
A


ATOM
909
CB
GLU
A
117
78.033
77.403
30.936
1.00
21.64
A


ATOM
910
CG
GLU
A
117
77.035
76.355
30.526
1.00
27.37
A


ATOM
911
CD
GLU
A
117
75.767
76.959
29.969
1.00
26.27
A


ATOM
912
OE1
GLU
A
117
75.449
78.124
30.316
1.00
25.92
A


ATOM
913
OE2
GLU
A
117
75.090
76.260
29.191
1.00
30.37
A


ATOM
914
C
GLU
A
117
79.257
79.457
30.470
1.00
24.39
A


ATOM
915
O
GLU
A
117
78.611
80.460
30.753
1.00
25.44
A


ATOM
916
N
LYS
A
118
80.561
79.373
30.706
1.00
18.78
A


ATOM
917
CA
LYS
A
118
81.189
80.460
31.440
1.00
19.49
A


ATOM
918
CB
LYS
A
118
81.782
79.887
32.748
1.00
25.65
A


ATOM
919
CG
LYS
A
118
80.761
79.090
33.606
1.00
17.16
A


ATOM
920
CD
LYS
A
118
79.665
80.070
34.058
1.00
21.32
A


ATOM
921
CE
LYS
A
118
78.463
79.410
34.663
1.00
23.09
A


ATOM
922
NZ
LYS
A
118
77.522
80.476
35.101
1.00
23.88
A


ATOM
923
C
LYS
A
118
82.213
81.332
30.744
1.00
22.37
A


ATOM
924
O
LYS
A
118
82.898
82.122
31.400
1.00
20.49
A


ATOM
925
N
GLN
A
119
82.360
81.202
29.431
1.00
20.57
A


ATOM
926
CA
GLN
A
119
83.332
82.060
28.748
1.00
32.06
A


ATOM
927
CB
GLN
A
119
84.242
81.238
27.801
1.00
20.00
A


ATOM
928
CG
GLN
A
119
85.382
82.091
27.173
1.00
20.89
A


ATOM
929
CD
GLN
A
119
86.339
81.257
26.337
1.00
23.85
A


ATOM
930
OE1
GLN
A
119
85.940
80.270
25.734
1.00
18.93
A


ATOM
931
NE2
GLN
A
119
87.602
81.661
26.285
1.00
15.38
A


ATOM
932
C
GLN
A
119
82.543
83.095
27.929
1.00
24.21
A


ATOM
933
O
GLN
A
119
81.649
82.725
27.195
1.00
21.97
A


ATOM
934
N
LYS
A
120
82.880
84.367
28.048
1.00
28.47
A


ATOM
935
CA
LYS
A
120
82.193
85.403
27.288
1.00
33.91
A


ATOM
936
CB
LYS
A
120
82.448
86.774
27.918
1.00
29.00
A


ATOM
937
CG
LYS
A
120
81.948
86.897
29.337
1.00
38.95
A


ATOM
938
CD
LYS
A
120
82.173
88.303
29.892
1.00
47.54
A


ATOM
939
CE
LYS
A
120
81.955
88.332
31.396
1.00
56.75
A


ATOM
940
NZ
LYS
A
120
80.623
87.776
31.790
1.00
65.69
A


ATOM
941
C
LYS
A
120
82.673
85.434
25.818
1.00
36.89
A


ATOM
942
O
LYS
A
120
83.700
84.864
25.460
1.00
30.22
A


ATOM
943
N
PRO
A
121
81.926
86.120
24.949
1.00
37.55
A


ATOM
944
CD
PRO
A
121
80.622
86.771
25.165
1.00
41.75
A


ATOM
945
CA
PRO
A
121
82.327
86.197
23.548
1.00
31.55
A


ATOM
946
CB
PRO
A
121
81.271
87.114
22.940
1.00
39.82
A


ATOM
947
CG
PRO
A
121
80.041
86.762
23.756
1.00
44.17
A


ATOM
948
C
PRO
A
121
83.724
86.780
23.443
1.00
31.58
A


ATOM
949
O
PRO
A
121
84.489
86.388
22.568
1.00
29.41
A


ATOM
950
N
ASP
A
122
84.076
87.693
24.342
1.00
27.25
A


ATOM
951
CA
ASP
A
122
85.401
88.285
24.268
1.00
29.04
A


ATOM
952
CB
ASP
A
122
85.435
89.636
24.985
1.00
28.38
A


ATOM
953
CG
ASP
A
122
85.281
89.525
26.494
1.00
38.36
A


ATOM
954
OD1
ASP
A
122
84.964
88.438
27.033
1.00
39.09
A


ATOM
955
OD2
ASP
A
122
85.477
90.558
27.156
1.00
38.98
A


ATOM
956
C
ASP
A
122
86.531
87.363
24.783
1.00
32.13
A


ATOM
957
O
ASP
A
122
87.693
87.759
24.780
1.00
26.62
A


ATOM
958
N
GLY
A
123
86.186
86.145
25.214
1.00
30.31
A


ATOM
959
CA
GLY
A
123
87.203
85.196
25.678
1.00
30.38
A


ATOM
960
C
GLY
A
123
87.422
85.098
27.188
1.00
30.19
A


ATOM
961
O
GLY
A
123
88.039
84.144
27.654
1.00
29.94
A


ATOM
962
N
VAL
A
124
86.930
86.077
27.940
1.00
24.05
A


ATOM
963
CA
VAL
A
124
87.055
86.116
29.403
1.00
23.87
A


ATOM
964
CB
VAL
A
124
86.546
87.473
29.951
1.00
23.70
A


ATOM
965
CG1
VAL
A
124
86.353
87.413
31.459
1.00
27.05
A


ATOM
966
CG2
VAL
A
124
87.522
88.581
29.581
1.00
24.79
A


ATOM
967
C
VAL
A
124
86.240
85.041
30.106
1.00
29.58
A


ATOM
968
O
VAL
A
124
85.071
84.813
29.733
1.00
26.81
A


ATOM
969
N
PHE
A
125
86.824
84.383
31.126
1.00
24.92
A


ATOM
970
CA
PHE
A
125
86.040
83.394
31.891
1.00
25.88
A


ATOM
971
CB
PHE
A
125
86.893
82.228
32.352
1.00
24.21
A


ATOM
972
CG
PHE
A
125
87.069
81.189
31.312
1.00
25.00
A


ATOM
973
CD1
PHE
A
125
86.059
80.277
31.059
1.00
23.52
A


ATOM
974
CD2
PHE
A
125
88.224
81.146
30.534
1.00
22.60
A


ATOM
975
CE1
PHE
A
125
86.186
79.345
30.063
1.00
23.68
A


ATOM
976
CE2
PHE
A
125
88.354
80.202
29.517
1.00
16.73
A


ATOM
977
CZ
PHE
A
125
87.338
79.306
29.282
1.00
20.14
A


ATOM
978
C
PHE
A
125
85.479
84.135
33.090
1.00
24.56
A


ATOM
979
O
PHE
A
125
86.175
84.941
33.710
1.00
21.73
A


ATOM
980
N
GLN
A
126
84.214
83.866
33.414
1.00
21.30
A


ATOM
981
CA
GLN
A
126
83.558
84.549
34.530
1.00
22.03
A


ATOM
982
CB
GLN
A
126
82.348
85.333
34.003
1.00
33.53
A


ATOM
983
CG
GLN
A
126
81.694
86.233
35.019
1.00
46.06
A


ATOM
984
CD
GLN
A
126
80.588
87.096
34.409
1.00
54.63
A


ATOM
985
OE1
GLN
A
126
79.474
86.612
34.131
1.00
56.00
A


ATOM
986
NE2
GLN
A
126
80.897
88.383
34.188
1.00
55.55
A


ATOM
987
C
GLN
A
126
83.085
83.607
35.634
1.00
18.80
A


ATOM
988
O
GLN
A
126
82.611
82.489
35.360
1.00
18.84
A


ATOM
989
N
GLU
A
127
83.202
84.068
36.871
1.00
19.63
A


ATOM
990
CA
GLU
A
127
82.770
83.301
38.050
1.00
18.00
A


ATOM
991
CB
GLU
A
127
83.708
83.576
39.232
1.00
17.47
A


ATOM
992
CG
GLU
A
127
83.190
82.969
40.556
1.00
21.51
A


ATOM
993
CD
GLU
A
127
83.051
81.442
40.446
1.00
23.16
A


ATOM
994
OE1
GLU
A
127
82.025
80.930
39.942
1.00
17.83
A


ATOM
995
OE2
GLU
A
127
84.008
80.754
40.848
1.00
21.73
A


ATOM
996
C
GLU
A
127
81.374
83.823
38.419
1.00
26.19
A


ATOM
997
O
GLU
A
127
81.232
85.016
38.663
1.00
21.32
A


ATOM
998
N
ASP
A
128
80.339
82.981
38.438
1.00
24.79
A


ATOM
999
CA
ASP
A
128
79.015
83.486
38.837
1.00
23.86
A


ATOM
1000
CB
ASP
A
128
77.909
83.020
37.876
1.00
28.03
A


ATOM
1001
CG
ASP
A
128
77.970
83.709
36.523
1.00
31.99
A


ATOM
1002
OD1
ASP
A
128
78.166
84.944
36.495
1.00
35.60
A


ATOM
1003
OD2
ASP
A
128
77.807
83.018
35.492
1.00
29.56
A


ATOM
1004
C
ASP
A
128
78.659
83.006
40.253
1.00
22.48
A


ATOM
1005
O
ASP
A
128
77.629
83.368
40.796
1.00
23.11
A


ATOM
1006
N
ALA
A
129
79.498
82.172
40.849
1.00
20.40
A


ATOM
1007
CA
ALA
A
129
79.215
81.676
42.210
1.00
25.25
A


ATOM
1008
CB
ALA
A
129
78.336
80.383
42.153
1.00
24.60
A


ATOM
1009
C
ALA
A
129
80.531
81.385
42.926
1.00
21.47
A


ATOM
1010
O
ALA
A
129
81.012
80.267
42.901
1.00
20.12
A


ATOM
1011
N
PRO
A
130
81.142
82.412
43.535
1.00
22.93
A


ATOM
1012
CD
PRO
A
130
80.707
83.830
43.484
1.00
18.89
A


ATOM
1013
CA
PRO
A
130
82.406
82.269
44.248
1.00
20.89
A


ATOM
1014
CB
PRO
A
130
82.610
83.637
44.868
1.00
24.77
A


ATOM
1015
CG
PRO
A
130
81.951
84.575
43.823
1.00
29.40
A


ATOM
1016
C
PRO
A
130
82.417
81.168
45.314
1.00
25.72
A


ATOM
1017
O
PRO
A
130
81.421
80.932
46.010
1.00
20.76
A


ATOM
1018
N
VAL
A
131
83.557
80.497
45.431
1.00
18.66
A


ATOM
1019
CA
VAL
A
131
83.709
79.442
46.421
1.00
25.11
A


ATOM
1020
CB
VAL
A
131
85.057
78.722
46.307
1.00
15.74
A


ATOM
1021
CG1
VAL
A
131
85.116
77.946
44.960
1.00
15.94
A


ATOM
1022
CG2
VAL
A
131
86.188
79.725
46.459
1.00
19.54
A


ATOM
1023
C
VAL
A
131
83.618
80.030
47.806
1.00
19.19
A


ATOM
1024
O
VAL
A
131
83.848
81.225
48.014
1.00
21.17
A


ATOM
1025
N
ILE
A
132
83.251
79.169
48.747
1.00
18.82
A


ATOM
1026
CA
ILE
A
132
83.149
79.556
50.154
1.00
23.91
A


ATOM
1027
CB
ILE
A
132
82.332
78.512
50.929
1.00
24.37
A


ATOM
1028
CG2
ILE
A
132
82.271
78.876
52.417
1.00
20.00
A


ATOM
1029
CG1
ILE
A
132
80.941
78.425
50.306
1.00
21.42
A


ATOM
1030
CD1
ILE
A
132
80.142
77.180
50.747
1.00
24.98
A


ATOM
1031
C
ILE
A
132
84.541
79.688
50.790
1.00
19.79
A


ATOM
1032
O
ILE
A
132
84.781
80.623
51.520
1.00
17.40
A


ATOM
1033
N
HIS
A
133
85.467
78.766
50.504
1.00
18.82
A


ATOM
1034
CA
HIS
A
133
86.827
78.851
51.100
1.00
17.96
A


ATOM
1035
CB
HIS
A
133
87.434
77.437
51.218
1.00
18.01
A


ATOM
1036
CG
HIS
A
133
86.691
76.548
52.180
1.00
22.75
A


ATOM
1037
CD2
HIS
A
133
85.617
75.738
51.996
1.00
18.28
A


ATOM
1038
ND1
HIS
A
133
86.992
76.499
53.531
1.00
25.58
A


ATOM
1039
CE1
HIS
A
133
86.128
75.693
54.137
1.00
21.42
A


ATOM
1040
NE2
HIS
A
133
85.289
75.222
53.231
1.00
27.04
A


ATOM
1041
C
HIS
A
133
87.711
79.735
50.221
1.00
14.42
A


ATOM
1042
O
HIS
A
133
88.525
79.250
49.431
1.00
17.65
A


ATOM
1043
N
GLN
A
134
87.517
81.039
50.373
1.00
11.52
A


ATOM
1044
CA
GLN
A
134
88.238
82.045
49.608
1.00
21.65
A


ATOM
1045
CB
GLN
A
134
87.689
83.424
50.025
1.00
17.09
A


ATOM
1046
CG
GLN
A
134
86.298
83.729
49.418
1.00
22.43
A


ATOM
1047
CD
GLN
A
134
86.376
84.059
47.908
1.00
21.78
A


ATOM
1048
OE1
GLN
A
134
87.129
84.944
47.522
1.00
28.45
A


ATOM
1049
NE2
GLN
A
134
85.604
83.340
47.061
1.00
22.37
A


ATOM
1050
C
GLN
A
134
89.759
81.873
49.837
1.00
20.34
A


ATOM
1051
O
GLN
A
134
90.565
82.314
49.052
1.00
26.33
A


ATOM
1052
N
GLU
A
135
90.079
81.179
50.928
1.00
19.09
A


ATOM
1053
CA
GLU
A
135
91.407
80.777
51.431
1.00
24.48
A


ATOM
1054
CB
GLU
A
135
91.169
79.850
52.767
1.00
24.93
A


ATOM
1055
CG
GLU
A
135
89.522
79.250
53.099
1.00
4.96
A


ATOM
1056
CD
GLU
A
135
89.446
77.808
53.925
1.00
27.66
A


ATOM
1057
OE1
GLU
A
135
90.527
77.338
53.831
1.00
37.42
A


ATOM
1058
OE2
GLU
A
135
88.528
77.190
54.620
1.00
1.00
A


ATOM
1059
C
GLU
A
135
92.140
79.938
50.318
1.00
20.82
A


ATOM
1060
O
GLU
A
135
93.362
80.014
50.119
1.00
15.84
A


ATOM
1061
N
MET
A
136
91.377
79.109
49.602
1.00
18.40
A


ATOM
1062
CA
MET
A
136
91.967
78.147
48.626
1.00
17.42
A


ATOM
1063
CB
MET
A
136
91.123
76.853
48.571
1.00
15.67
A


ATOM
1064
CG
MET
A
136
89.868
76.961
47.675
1.00
17.99
A


ATOM
1065
SD
MET
A
136
88.703
75.563
47.631
1.00
29.44
A


ATOM
1066
CE
MET
A
136
89.744
74.260
47.009
1.00
11.97
A


ATOM
1067
C
MET
A
136
92.269
78.546
47.203
1.00
15.51
A


ATOM
1068
O
MET
A
136
92.791
77.747
46.443
1.00
14.46
A


ATOM
1069
N
ILE
A
137
91.988
79.787
46.831
1.00
16.94
A


ATOM
1070
CA
ILE
A
137
92.217
80.213
45.449
1.00
15.24
A


ATOM
1071
CB
ILE
A
137
90.980
80.959
44.955
1.00
20.19
A


ATOM
1072
CG2
ILE
A
137
89.724
80.121
45.254
1.00
14.68
A


ATOM
1073
CG1
ILE
A
137
90.847
82.300
45.723
1.00
11.83
A


ATOM
1074
CD1
ILE
A
137
89.473
82.985
45.435
1.00
23.22
A


ATOM
1075
C
ILE
A
137
93.446
81.121
45.287
1.00
19.40
A


ATOM
1076
O
ILE
A
137
93.658
81.729
44.228
1.00
18.10
A


ATOM
1077
N
GLY
A
138
94.222
81.215
46.361
1.00
15.44
A


ATOM
1078
CA
GLY
A
138
95.438
82.007
46.314
1.00
18.65
A


ATOM
1079
C
GLY
A
138
95.197
83.450
45.897
1.00
23.13
A


ATOM
1080
O
GLY
A
138
94.182
84.061
46.313
1.00
15.00
A


ATOM
1081
N
GLY
A
139
96.097
83.962
45.044
1.00
20.15
A


ATOM
1082
CA
GLY
A
139
96.054
85.338
44.585
1.00
17.13
A


ATOM
1083
C
GLY
A
139
94.767
85.808
43.967
1.00
29.17
A


ATOM
1084
O
GLY
A
139
94.568
87.013
43.807
1.00
33.25
A


ATOM
1085
N
LEU
A
140
93.875
84.890
43.618
1.00
23.37
A


ATOM
1086
CA
LEU
A
140
92.621
85.317
43.023
1.00
24.08
A


ATOM
1087
CB
LEU
A
140
91.930
84.142
42.322
1.00
22.29
A


ATOM
1088
CG
LEU
A
140
92.000
84.065
40.800
1.00
36.28
A


ATOM
1089
CD1
LEU
A
140
93.365
84.425
40.297
1.00
39.55
A


ATOM
1090
CD2
LEU
A
140
91.640
82.663
40.376
1.00
31.19
A


ATOM
1091
C
LEU
A
140
91.682
85.918
44.065
1.00
30.45
A


ATOM
1092
O
LEU
A
140
90.698
86.572
43.697
1.00
29.82
A


ATOM
1093
N
ARG
A
141
91.977
85.715
45.353
1.00
23.95
A


ATOM
1094
CA
ARG
A
141
91.105
86.235
46.411
1.00
26.90
A


ATOM
1095
CB
ARG
A
141
91.577
85.782
47.790
1.00
23.51
A


ATOM
1096
CG
ARG
A
141
90.622
86.183
48.883
1.00
31.94
A


ATOM
1097
CD
ARG
A
141
91.045
85.655
50.222
1.00
41.48
A


ATOM
1098
NE
ARG
A
141
90.058
85.983
51.254
1.00
56.61
A


ATOM
1099
CZ
ARG
A
141
89.989
85.390
52.451
1.00
62.59
A


ATOM
1100
NH1
ARG
A
141
90.853
84.427
52.773
1.00
61.70
A


ATOM
1101
NH2
ARG
A
141
89.055
85.761
53.330
1.00
64.29
A


ATOM
1102
C
ARG
A
141
91.103
87.771
46.308
1.00
31.97
A


ATOM
1103
O
ARG
A
141
90.112
88.440
46.571
1.00
35.29
A


ATOM
1104
N
ASN
A
142
92.233
88.327
45.932
1.00
35.39
A


ATOM
1105
CA
ASN
A
142
92.292
89.759
45.708
1.00
47.29
A


ATOM
1106
CB
ASN
A
142
93.755
90.199
45.620
1.00
44.19
A


ATOM
1107
CG
ASN
A
142
93.903
91.672
45.316
1.00
50.84
A


ATOM
1108
OD1
ASN
A
142
93.081
92.260
44.606
1.00
50.20
A


ATOM
1109
ND2
ASN
A
142
94.968
92.273
45.829
1.00
52.23
A


ATOM
1110
C
ASN
A
142
91.588
89.889
44.342
1.00
43.41
A


ATOM
1111
O
ASN
A
142
92.160
89.542
43.302
1.00
41.71
A


ATOM
1112
N
ASN
A
143
90.343
90.361
44.363
1.00
49.05
A


ATOM
1113
CA
ASN
A
143
89.511
90.522
43.151
1.00
54.92
A


ATOM
1114
CB
ASN
A
143
88.071
90.941
43.523
1.00
58.67
A


ATOM
1115
CG
ASN
A
143
87.097
89.762
43.556
1.00
67.74
A


ATOM
1116
OD1
ASN
A
143
87.173
88.843
42.721
1.00
72.16
A


ATOM
1117
ND2
ASN
A
143
86.159
89.795
44.510
1.00
73.35
A


ATOM
1118
C
ASN
A
143
90.024
91.509
42.102
1.00
51.69
A


ATOM
1119
O
ASN
A
143
89.457
91.625
41.012
1.00
52.71
A


ATOM
1120
N
ASN
A
144
91.067
92.245
42.432
1.00
47.25
A


ATOM
1121
CA
ASN
A
144
91.597
93.191
41.479
1.00
47.83
A


ATOM
1122
CB
ASN
A
144
92.706
93.993
42.126
1.00
58.20
A


ATOM
1123
CG
ASN
A
144
92.406
95.451
42.142
1.00
67.49
A


ATOM
1124
OD1
ASN
A
144
92.353
96.070
43.210
1.00
75.34
A


ATOM
1125
ND2
ASN
A
144
92.194
96.027
40.954
1.00
70.42
A


ATOM
1126
C
ASN
A
144
92.157
92.456
40.274
1.00
42.44
A


ATOM
1127
O
ASN
A
144
92.967
91.540
40.432
1.00
43.00
A


ATOM
1128
N
GLU
A
145
91.740
92.866
39.081
1.00
35.72
A


ATOM
1129
CA
GLU
A
145
92.207
92.277
37.832
1.00
30.80
A


ATOM
1130
CB
GLU
A
145
93.751
92.269
37.777
1.00
29.82
A


ATOM
1131
CG
GLU
A
145
94.342
93.705
37.873
1.00
34.98
A


ATOM
1132
CD
GLU
A
145
95.866
93.839
37.711
1.00
33.90
A


ATOM
1133
OE1
GLU
A
145
96.311
94.948
37.366
1.00
37.12
A


ATOM
1134
OE2
GLU
A
145
96.641
92.890
37.938
1.00
36.12
A


ATOM
1135
C
GLU
A
145
91.639
90.883
37.705
1.00
32.53
A


ATOM
1136
O
GLU
A
145
92.261
89.979
37.134
1.00
29.47
A


ATOM
1137
N
LYS
A
146
90.410
90.715
38.179
1.00
33.08
A


ATOM
1138
CA
LYS
A
146
89.829
89.386
38.144
1.00
34.99
A


ATOM
1139
CB
LYS
A
146
88.545
89.324
38.969
1.00
38.84
A


ATOM
1140
CG
LYS
A
146
87.328
89.843
38.309
1.00
46.43
A


ATOM
1141
CD
LYS
A
146
86.173
89.733
39.312
1.00
53.97
A


ATOM
1142
CE
LYS
A
146
84.799
89.902
38.655
1.00
56.22
A


ATOM
1143
NZ
LYS
A
146
84.630
91.185
37.909
1.00
58.13
A


ATOM
1144
C
LYS
A
146
89.597
88.782
36.778
1.00
29.69
A


ATOM
1145
O
LYS
A
146
89.864
87.593
36.608
1.00
22.87
A


ATOM
1146
N
ASP
A
147
89.125
89.558
35.800
1.00
26.03
A


ATOM
1147
CA
ASP
A
147
88.883
88.989
34.459
1.00
24.17
A


ATOM
1148
CB
ASP
A
147
88.363
90.051
33.483
1.00
26.31
A


ATOM
1149
CG
ASP
A
147
86.884
90.374
33.687
1.00
33.37
A


ATOM
1150
OD1
ASP
A
147
86.419
91.396
33.148
1.00
41.72
A


ATOM
1151
OD2
ASP
A
147
86.160
89.619
34.369
1.00
28.33
A


ATOM
1152
C
ASP
A
147
90.175
88.402
33.894
1.00
29.67
A


ATOM
1153
O
ASP
A
147
90.177
87.328
33.291
1.00
30.44
A


ATOM
1154
N
MET
A
148
91.277
89.112
34.087
1.00
21.79
A


ATOM
1155
CA
MET
A
148
92.562
88.631
33.567
1.00
27.79
A


ATOM
1156
CB
MET
A
148
93.591
89.770
33.532
1.00
21.45
A


ATOM
1157
CG
MET
A
148
93.406
90.794
32.411
1.00
16.88
A


ATOM
1158
SD
MET
A
148
94.007
90.171
30.836
1.00
26.17
A


ATOM
1159
CE
MET
A
148
95.843
90.114
31.224
1.00
25.36
A


ATOM
1160
C
MET
A
148
93.097
87.466
34.431
1.00
23.06
A


ATOM
1161
O
MET
A
148
93.502
86.433
33.902
1.00
26.32
A


ATOM
1162
N
ALA
A
149
93.082
87.630
35.749
1.00
21.95
A


ATOM
1163
CA
ALA
A
149
93.577
86.584
36.637
1.00
26.30
A


ATOM
1164
CB
ALA
A
149
93.539
87.058
38.093
1.00
17.92
A


ATOM
1165
C
ALA
A
149
92.762
85.290
36.464
1.00
24.09
A


ATOM
1166
O
ALA
A
149
93.336
84.239
36.228
1.00
20.02
A


ATOM
1167
N
LEU
A
150
91.441
85.357
36.578
1.00
23.60
A


ATOM
1168
CA
LEU
A
150
90.615
84.157
36.396
1.00
21.51
A


ATOM
1169
CB
LEU
A
150
89.141
84.431
36.708
1.00
21.03
A


ATOM
1170
CG
LEU
A
150
88.167
83.219
36.546
1.00
31.38
A


ATOM
1171
CD1
LEU
A
150
88.557
82.048
37.474
1.00
23.78
A


ATOM
1172
CD2
LEU
A
150
86.725
83.681
36.903
1.00
28.01
A


ATOM
1173
C
LEU
A
150
90.707
83.581
34.985
1.00
26.10
A


ATOM
1174
O
LEU
A
150
90.700
82.351
34.794
1.00
23.93
A


ATOM
1175
N
THR
A
151
90.777
84.442
33.978
1.00
21.82
A


ATOM
1176
CA
THR
A
151
90.895
83.927
32.622
1.00
16.71
A


ATOM
1177
CB
THR
A
151
90.817
85.079
31.570
1.00
24.41
A


ATOM
1178
OG1
THR
A
151
89.512
85.680
31.646
1.00
24.76
A


ATOM
1179
CG2
THR
A
151
91.004
84.529
30.144
1.00
24.82
A


ATOM
1180
C
THR
A
151
92.199
83.146
32.466
1.00
22.41
A


ATOM
1181
O
THR
A
151
92.214
82.096
31.853
1.00
22.92
A


ATOM
1182
N
ALA
A
152
93.283
83.633
33.055
1.00
26.27
A


ATOM
1183
CA
ALA
A
152
94.557
82.926
32.945
1.00
19.92
A


ATOM
1184
CB
ALA
A
152
95.654
83.757
33.543
1.00
15.47
A


ATOM
1185
C
ALA
A
152
94.459
81.578
33.671
1.00
19.39
A


ATOM
1186
O
ALA
A
152
94.882
80.567
33.133
1.00
19.38
A


ATOM
1187
N
PHE
A
153
93.884
81.603
34.877
1.00
17.38
A


ATOM
1188
CA
PHE
A
153
93.713
80.417
35.703
1.00
14.60
A


ATOM
1189
CB
PHE
A
153
92.970
80.716
37.004
1.00
17.50
A


ATOM
1190
CG
PHE
A
153
92.773
79.492
37.851
1.00
22.29
A


ATOM
1191
CD1
PHE
A
153
93.813
79.005
38.652
1.00
10.97
A


ATOM
1192
CD2
PHE
A
153
91.551
78.828
37.855
1.00
15.16
A


ATOM
1193
CE1
PHE
A
153
93.623
77.853
39.448
1.00
20.47
A


ATOM
1194
CE2
PHE
A
153
91.348
77.689
38.636
1.00
22.68
A


ATOM
1195
CZ
PHE
A
153
92.387
77.205
39.442
1.00
21.15
A


ATOM
1196
C
PHE
A
153
92.940
79.346
34.977
1.00
19.47
A


ATOM
1197
O
PHE
A
153
93.360
78.219
34.971
1.00
18.15
A


ATOM
1198
N
VAL
A
154
91.805
79.687
34.388
1.00
17.18
A


ATOM
1199
CA
VAL
A
154
91.016
78.686
33.682
1.00
14.37
A


ATOM
1200
CB
VAL
A
154
89.598
79.241
33.313
1.00
17.63
A


ATOM
1201
CG1
VAL
A
154
88.830
78.188
32.527
1.00
14.29
A


ATOM
1202
CG2
VAL
A
154
88.835
79.585
34.588
1.00
17.52
A


ATOM
1203
C
VAL
A
154
91.750
78.197
32.416
1.00
19.75
A


ATOM
1204
O
VAL
A
154
91.801
77.003
32.146
1.00
15.15
A


ATOM
1205
N
LEU
A
155
92.336
79.109
31.652
1.00
20.48
A


ATOM
1206
CA
LEU
A
155
93.093
78.710
30.457
1.00
22.98
A


ATOM
1207
CB
LEU
A
155
93.729
79.960
29.791
1.00
16.47
A


ATOM
1208
CG
LEU
A
155
94.688
79.745
28.623
1.00
19.59
A


ATOM
1209
CD1
LEU
A
155
94.015
78.920
27.570
1.00
17.74
A


ATOM
1210
CD2
LEU
A
155
95.164
81.108
28.034
1.00
22.48
A


ATOM
1211
C
LEU
A
155
94.200
77.680
30.800
1.00
16.38
A


ATOM
1212
O
LEU
A
155
94.391
76.711
30.079
1.00
21.06
A


ATOM
1213
N
ILE
A
156
94.926
77.903
31.897
1.00
17.56
A


ATOM
1214
CA
ILE
A
156
96.016
76.992
32.288
1.00
21.21
A


ATOM
1215
CB
ILE
A
156
96.769
77.543
33.538
1.00
23.70
A


ATOM
1216
CG2
ILE
A
156
97.704
76.490
34.155
1.00
17.82
A


ATOM
1217
CG1
ILE
A
156
97.584
78.768
33.108
1.00
20.22
A


ATOM
1218
CD1
ILE
A
156
98.096
79.593
34.297
1.00
15.17
A


ATOM
1219
C
ILE
A
156
95.392
75.608
32.555
1.00
21.00
A


ATOM
1220
O
ILE
A
156
95.955
74.595
32.181
1.00
18.43
A


ATOM
1221
N
SER
A
157
94.222
75.577
33.187
1.00
14.32
A


ATOM
1222
CA
SER
A
157
93.525
74.316
33.425
1.00
22.61
A


ATOM
1223
CB
SER
A
157
92.244
74.564
34.218
1.00
23.22
A


ATOM
1224
OG
SER
A
157
92.563
74.830
35.582
1.00
30.07
A


ATOM
1225
C
SER
A
157
93.174
73.644
32.105
1.00
19.13
A


ATOM
1226
O
SER
A
157
93.405
72.445
31.924
1.00
23.24
A


ATOM
1227
N
LEU
A
158
92.640
74.416
31.163
1.00
23.19
A


ATOM
1228
CA
LEU
A
158
92.268
73.872
29.859
1.00
26.55
A


ATOM
1229
CB
LEU
A
158
91.607
74.955
28.970
1.00
23.19
A


ATOM
1230
CG
LEU
A
158
90.330
75.603
29.537
1.00
28.10
A


ATOM
1231
CD1
LEU
A
158
89.618
76.320
28.444
1.00
30.58
A


ATOM
1232
CD2
LEU
A
158
89.378
74.573
30.117
1.00
26.46
A


ATOM
1233
C
LEU
A
158
93.464
73.293
29.126
1.00
23.77
A


ATOM
1234
O
LEU
A
158
93.342
72.294
28.417
1.00
20.25
A


ATOM
1235
N
GLN
A
159
94.622
73.927
29.269
1.00
20.67
A


ATOM
1236
CA
GLN
A
159
95.823
73.433
28.580
1.00
19.57
A


ATOM
1237
CB
GLN
A
159
96.995
74.418
28.776
1.00
20.64
A


ATOM
1238
CG
GLN
A
159
96.787
75.764
28.118
1.00
25.42
A


ATOM
1239
CD
GLN
A
159
97.003
75.741
26.600
1.00
26.92
A


ATOM
1240
OE1
GLN
A
159
96.865
74.703
25.933
1.00
24.64
A


ATOM
1241
NE2
GLN
A
159
97.341
76.899
26.049
1.00
24.27
A


ATOM
1242
C
GLN
A
159
96.195
72.047
29.136
1.00
25.83
A


ATOM
1243
O
GLN
A
159
96.681
71.182
28.390
1.00
22.57
A


ATOM
1244
N
GLU
A
160
95.963
71.833
30.433
1.00
18.37
A


ATOM
1245
CA
GLU
A
160
96.275
70.533
31.010
1.00
28.44
A


ATOM
1246
CB
GLU
A
160
96.124
70.505
32.527
1.00
18.70
A


ATOM
1247
CG
GLU
A
160
97.204
71.226
33.289
1.00
29.07
A


ATOM
1248
CD
GLU
A
160
97.071
71.010
34.792
1.00
37.12
A


ATOM
1249
OE1
GLU
A
160
96.376
70.046
35.219
1.00
29.05
A


ATOM
1250
OE2
GLU
A
160
97.673
71.806
35.539
1.00
41.97
A


ATOM
1251
C
GLU
A
160
95.364
69.454
30.478
1.00
29.40
A


ATOM
1252
O
GLU
A
160
95.782
68.316
30.357
1.00
24.26
A


ATOM
1253
N
ALA
A
161
94.110
69.796
30.177
1.00
25.80
A


ATOM
1254
CA
ALA
A
161
93.182
68.775
29.705
1.00
26.76
A


ATOM
1255
CB
ALA
A
161
91.827
68.987
30.370
1.00
25.94
A


ATOM
1256
C
ALA
A
161
93.019
68.749
28.189
1.00
29.95
A


ATOM
1257
O
ALA
A
161
92.166
68.029
27.654
1.00
32.80
A


ATOM
1258
N
LYS
A
162
93.848
69.514
27.492
1.00
30.99
A


ATOM
1259
CA
LYS
A
162
93.741
69.628
26.043
1.00
37.27
A


ATOM
1260
CB
LYS
A
162
94.762
70.671
25.553
1.00
38.61
A


ATOM
1261
CG
LYS
A
162
94.792
70.892
24.048
1.00
50.32
A


ATOM
1262
CD
LYS
A
162
95.668
72.093
23.628
1.00
56.78
A


ATOM
1263
CE
LYS
A
162
97.171
71.809
23.618
1.00
58.73
A


ATOM
1264
NZ
LYS
A
162
97.593
70.975
22.433
1.00
67.24
A


ATOM
1265
C
LYS
A
162
93.885
68.321
25.254
1.00
37.81
A


ATOM
1266
O
LYS
A
162
93.029
67.964
24.455
1.00
33.39
A


ATOM
1267
N
ASP
A
163
94.968
67.601
25.475
1.00
41.14
A


ATOM
1268
CA
ASP
A
163
95.171
66.387
24.719
1.00
42.49
A


ATOM
1269
CB
ASP
A
163
96.576
65.858
24.965
1.00
51.70
A


ATOM
1270
CG
ASP
A
163
97.643
66.839
24.512
1.00
57.18
A


ATOM
1271
OD1
ASP
A
163
97.366
67.598
23.557
1.00
62.80
A


ATOM
1272
OD2
ASP
A
163
98.752
66.846
25.094
1.00
64.82
A


ATOM
1273
C
ASP
A
163
94.127
65.361
25.074
1.00
41.86
A


ATOM
1274
O
ASP
A
163
93.582
64.687
24.214
1.00
41.54
A


ATOM
1275
N
ILE
A
164
93.808
65.253
26.344
1.00
37.18
A


ATOM
1276
CA
ILE
A
164
92.801
64.297
26.721
1.00
37.23
A


ATOM
1277
CB
ILE
A
164
92.750
64.158
28.236
1.00
43.08
A


ATOM
1278
CG2
ILE
A
164
91.530
63.365
28.661
1.00
42.32
A


ATOM
1279
CG1
ILE
A
164
94.054
63.514
28.705
1.00
48.60
A


ATOM
1280
CD1
ILE
A
164
94.078
63.174
30.156
1.00
56.10
A


ATOM
1281
C
ILE
A
164
91.404
64.634
26.216
1.00
39.86
A


ATOM
1282
O
ILE
A
164
90.656
63.748
25.830
1.00
33.04
A


ATOM
1283
N
CYS
A
165
91.060
65.914
26.183
1.00
32.76
A


ATOM
1284
CA
CYS
A
165
89.715
66.278
25.814
1.00
34.31
A


ATOM
1285
C
CYS
A
165
89.433
66.817
24.422
1.00
37.19
A


ATOM
1286
O
CYS
A
165
88.287
66.818
24.011
1.00
28.15
A


ATOM
1287
CB
CYS
A
165
89.180
67.278
26.839
1.00
29.46
A


ATOM
1288
SG
CYS
A
165
89.010
66.566
28.503
1.00
28.08
A


ATOM
1289
N
GLU
A
166
90.459
67.264
23.707
1.00
45.15
A


ATOM
1290
CA
GLU
A
166
90.273
67.877
22.395
1.00
51.11
A


ATOM
1291
CB
GLU
A
166
91.630
68.143
21.720
1.00
59.07
A


ATOM
1292
CG
GLU
A
166
92.542
66.935
21.528
1.00
66.72
A


ATOM
1293
CD
GLU
A
166
93.935
67.335
21.000
1.00
71.70
A


ATOM
1294
OE1
GLU
A
166
94.838
66.457
20.967
1.00
74.38
A


ATOM
1295
OE2
GLU
A
166
94.123
68.526
20.619
1.00
68.32
A


ATOM
1296
C
GLU
A
166
89.349
67.152
21.449
1.00
50.87
A


ATOM
1297
O
GLU
A
166
88.661
67.784
20.646
1.00
50.00
A


ATOM
1298
N
GLU
A
167
89.314
65.833
21.559
1.00
52.19
A


ATOM
1299
CA
GLU
A
167
88.448
65.027
20.718
1.00
52.32
A


ATOM
1300
CB
GLU
A
167
88.840
63.559
20.838
1.00
60.45
A


ATOM
1301
CG
GLU
A
167
89.074
62.885
19.504
1.00
68.41
A


ATOM
1302
CD
GLU
A
167
89.979
63.713
18.605
1.00
74.42
A


ATOM
1303
OE1
GLU
A
167
91.048
64.161
19.082
1.00
77.67
A


ATOM
1304
OE2
GLU
A
167
89.621
63.917
17.421
1.00
78.62
A


ATOM
1305
C
GLU
A
167
86.963
65.177
21.059
1.00
54.06
A


ATOM
1306
O
GLU
A
167
86.136
65.356
20.163
1.00
52.37
A


ATOM
1307
N
GLN
A
168
86.632
65.103
22.351
1.00
46.71
A


ATOM
1308
CA
GLN
A
168
85.247
65.202
22.811
1.00
43.91
A


ATOM
1309
CB
GLN
A
168
85.059
64.480
24.155
1.00
50.94
A


ATOM
1310
CG
GLN
A
168
86.269
64.533
25.087
1.00
56.41
A


ATOM
1311
CD
GLN
A
168
87.154
63.284
24.976
1.00
55.96
A


ATOM
1312
OE1
GLN
A
168
86.830
62.226
25.526
1.00
49.92
A


ATOM
1313
NE2
GLN
A
168
88.268
63.406
24.247
1.00
57.62
A


ATOM
1314
C
GLN
A
168
84.752
66.620
22.959
1.00
42.37
A


ATOM
1315
O
GLN
A
168
83.547
66.856
23.073
1.00
41.71
A


ATOM
1316
N
VAL
A
169
85.673
67.571
22.965
1.00
33.31
A


ATOM
1317
CA
VAL
A
169
85.281
68.962
23.112
1.00
35.06
A


ATOM
1318
CB
VAL
A
169
85.809
69.506
24.469
1.00
27.42
A


ATOM
1319
CG1
VAL
A
169
85.245
70.889
24.754
1.00
25.13
A


ATOM
1320
CG2
VAL
A
169
85.402
68.540
25.573
1.00
24.56
A


ATOM
1321
C
VAL
A
169
85.780
69.792
21.919
1.00
34.13
A


ATOM
1322
O
VAL
A
169
86.866
70.376
21.962
1.00
31.73
A


ATOM
1323
N
ASN
A
170
84.971
69.841
20.857
1.00
31.12
A


ATOM
1324
CA
ASN
A
170
85.367
70.554
19.646
1.00
31.56
A


ATOM
1325
CB
ASN
A
170
84.377
70.283
18.503
1.00
38.49
A


ATOM
1326
CG
ASN
A
170
84.352
68.798
18.058
1.00
44.51
A


ATOM
1327
OD1
ASN
A
170
83.419
68.063
18.367
1.00
50.11
A


ATOM
1328
ND2
ASN
A
170
85.374
68.375
17.334
1.00
40.85
A


ATOM
1329
C
ASN
A
170
85.562
72.052
19.838
1.00
33.38
A


ATOM
1330
O
ASN
A
170
86.308
72.682
19.101
1.00
32.60
A


ATOM
1331
N
SER
A
171
84.931
72.641
20.843
1.00
29.65
A


ATOM
1332
CA
SER
A
171
85.123
74.080
21.054
1.00
27.07
A


ATOM
1333
CB
SER
A
171
83.937
74.650
21.858
1.00
26.83
A


ATOM
1334
OG
SER
A
171
83.675
73.861
23.020
1.00
22.03
A


ATOM
1335
C
SER
A
171
86.434
74.410
21.776
1.00
23.40
A


ATOM
1336
O
SER
A
171
86.829
75.571
21.868
1.00
25.17
A


ATOM
1337
N
LEU
A
172
87.130
73.400
22.274
1.00
20.64
A


ATOM
1338
CA
LEU
A
172
88.364
73.670
23.024
1.00
18.28
A


ATOM
1339
CB
LEU
A
172
88.976
72.340
23.451
1.00
26.95
A


ATOM
1340
CG
LEU
A
172
89.987
72.262
24.605
1.00
36.59
A


ATOM
1341
CD1
LEU
A
172
89.724
73.283
25.714
1.00
28.04
A


ATOM
1342
CD2
LEU
A
172
89.912
70.840
25.159
1.00
30.83
A


ATOM
1343
C
LEU
A
172
89.420
74.540
22.325
1.00
23.79
A


ATOM
1344
O
LEU
A
172
89.867
75.551
22.863
1.00
22.52
A


ATOM
1345
N
PRO
A
173
89.821
74.173
21.095
1.00
28.78
A


ATOM
1346
CD
PRO
A
173
89.392
73.036
20.258
1.00
26.81
A


ATOM
1347
CA
PRO
A
173
90.837
74.991
20.419
1.00
28.96
A


ATOM
1348
CB
PRO
A
173
91.001
74.308
19.054
1.00
28.65
A


ATOM
1349
CG
PRO
A
173
90.597
72.878
19.321
1.00
33.10
A


ATOM
1350
C
PRO
A
173
90.454
76.475
20.301
1.00
27.14
A


ATOM
1351
O
PRO
A
173
91.259
77.366
20.599
1.00
22.88
A


ATOM
1352
N
GLY
A
174
89.223
76.730
19.885
1.00
24.04
A


ATOM
1353
CA
GLY
A
174
88.771
78.104
19.731
1.00
24.51
A


ATOM
1354
C
GLY
A
174
88.667
78.805
21.071
1.00
23.82
A


ATOM
1355
O
GLY
A
174
88.943
79.985
21.172
1.00
24.00
A


ATOM
1356
N
SER
A
175
88.269
78.083
22.115
1.00
23.65
A


ATOM
1357
CA
SER
A
175
88.167
78.706
23.462
1.00
25.41
A


ATOM
1358
CB
SER
A
175
87.662
77.665
24.489
1.00
21.04
A


ATOM
1359
OG
SER
A
175
87.648
78.213
25.791
1.00
22.86
A


ATOM
1360
C
SER
A
175
89.534
79.224
23.917
1.00
23.64
A


ATOM
1361
O
SER
A
175
89.691
80.347
24.440
1.00
21.39
A


ATOM
1362
N
ILE
A
176
90.531
78.372
23.741
1.00
24.52
A


ATOM
1363
CA
ILE
A
176
91.907
78.683
24.127
1.00
20.94
A


ATOM
1364
CB
ILE
A
176
92.774
77.406
23.873
1.00
19.98
A


ATOM
1365
CG2
ILE
A
176
94.244
77.741
23.797
1.00
20.82
A


ATOM
1366
CG1
ILE
A
176
92.449
76.370
24.970
1.00
23.34
A


ATOM
1367
CD1
ILE
A
176
93.191
75.017
24.797
1.00
23.21
A


ATOM
1368
C
ILE
A
176
92.427
79.921
23.371
1.00
23.29
A


ATOM
1369
O
ILE
A
176
93.011
80.845
23.953
1.00
21.00
A


ATOM
1370
N
THR
A
177
92.180
79.957
22.069
1.00
22.38
A


ATOM
1371
CA
THR
A
177
92.622
81.096
21.280
1.00
26.70
A


ATOM
1372
CB
THR
A
177
92.298
80.826
19.810
1.00
30.44
A


ATOM
1373
OG1
THR
A
177
93.142
79.754
19.352
1.00
32.66
A


ATOM
1374
CG2
THR
A
177
92.507
82.071
18.959
1.00
25.97
A


ATOM
1375
C
THR
A
177
91.966
82.395
21.793
1.00
28.53
A


ATOM
1376
O
THR
A
177
92.635
83.437
21.992
1.00
25.08
A


ATOM
1377
N
LYS
A
178
90.665
82.320
22.058
1.00
24.69
A


ATOM
1378
CA
LYS
A
178
89.966
83.486
22.556
1.00
22.85
A


ATOM
1379
CB
LYS
A
178
88.458
83.212
22.647
1.00
33.69
A


ATOM
1380
CG
LYS
A
178
87.719
83.233
21.285
1.00
37.81
A


ATOM
1381
CD
LYS
A
178
86.260
82.880
21.478
1.00
45.50
A


ATOM
1382
CE
LYS
A
178
85.580
82.539
20.168
1.00
55.66
A


ATOM
1383
NZ
LYS
A
178
84.321
81.738
20.375
1.00
62.92
A


ATOM
1384
C
LYS
A
178
90.513
83.903
23.908
1.00
27.56
A


ATOM
1385
O
LYS
A
178
90.764
85.100
24.139
1.00
22.42
A


ATOM
1386
N
ALA
A
179
90.708
82.945
24.818
1.00
21.82
A


ATOM
1387
CA
ALA
A
179
91.232
83.327
26.117
1.00
22.17
A


ATOM
1388
CB
ALA
A
179
91.326
82.107
27.056
1.00
20.58
A


ATOM
1389
C
ALA
A
179
92.607
83.918
25.879
1.00
23.19
A


ATOM
1390
O
ALA
A
179
92.958
84.970
26.424
1.00
21.73
A


ATOM
1391
N
GLY
A
180
93.392
83.244
25.046
1.00
26.28
A


ATOM
1392
CA
GLY
A
180
94.732
83.741
24.790
1.00
25.77
A


ATOM
1393
C
GLY
A
180
94.723
85.155
24.206
1.00
29.69
A


ATOM
1394
O
GLY
A
180
95.509
86.005
24.628
1.00
26.61
A


ATOM
1395
N
ASP
A
181
93.869
85.403
23.215
1.00
19.22
A


ATOM
1396
CA
ASP
A
181
93.776
86.745
22.600
1.00
24.71
A


ATOM
1397
CB
ASP
A
181
92.670
86.781
21.544
1.00
28.40
A


ATOM
1398
CG
ASP
A
181
93.045
86.061
20.266
1.00
24.26
A


ATOM
1399
OD1
ASP
A
181
94.242
85.821
20.035
1.00
26.47
A


ATOM
1400
OD2
ASP
A
181
92.138
85.751
19.475
1.00
31.29
A


ATOM
1401
C
ASP
A
181
93.465
87.814
23.635
1.00
23.38
A


ATOM
1402
O
ASP
A
181
94.051
88.897
23.619
1.00
24.92
A


ATOM
1403
N
PHE
A
182
92.541
87.508
24.548
1.00
23.93
A


ATOM
1404
CA
PHE
A
182
92.179
88.481
25.562
1.00
24.17
A


ATOM
1405
CB
PHE
A
182
90.971
88.008
26.368
1.00
29.71
A


ATOM
1406
CG
PHE
A
182
90.573
88.970
27.457
1.00
37.61
A


ATOM
1407
CD1
PHE
A
182
89.777
90.074
27.174
1.00
35.83
A


ATOM
1408
CD2
PHE
A
182
91.034
88.798
28.751
1.00
37.07
A


ATOM
1409
CE1
PHE
A
182
89.447
90.989
28.165
1.00
38.94
A


ATOM
1410
CE2
PHE
A
182
90.706
89.712
29.748
1.00
42.65
A


ATOM
1411
CZ
PHE
A
182
89.915
90.806
29.456
1.00
41.10
A


ATOM
1412
C
PHE
A
182
93.343
88.759
26.514
1.00
29.73
A


ATOM
1413
O
PHE
A
182
93.610
89.911
26.877
1.00
26.64
A


ATOM
1414
N
LEU
A
183
94.041
87.710
26.933
1.00
23.11
A


ATOM
1415
CA
LEU
A
183
95.163
87.923
27.847
1.00
23.03
A


ATOM
1416
CB
LEU
A
183
95.794
86.587
28.302
1.00
19.13
A


ATOM
1417
CG
LEU
A
183
94.927
85.684
29.181
1.00
24.04
A


ATOM
1418
CD1
LEU
A
183
95.697
84.363
29.487
1.00
23.39
A


ATOM
1419
CD2
LEU
A
183
94.595
86.398
30.489
1.00
21.06
A


ATOM
1420
C
LEU
A
183
96.237
88.742
27.140
1.00
19.58
A


ATOM
1421
O
LEU
A
183
96.797
89.668
27.711
1.00
23.84
A


ATOM
1422
N
GLU
A
184
96.545
88.383
25.904
1.00
19.16
A


ATOM
1423
CA
GLU
A
184
97.584
89.114
25.190
1.00
29.47
A


ATOM
1424
CB
GLU
A
184
97.874
88.459
23.842
1.00
27.07
A


ATOM
1425
CG
GLU
A
184
98.874
89.217
23.010
1.00
33.26
A


ATOM
1426
CD
GLU
A
184
99.360
88.416
21.817
1.00
36.59
A


ATOM
1427
OE1
GLU
A
184
98.869
87.295
21.569
1.00
37.18
A


ATOM
1428
OE2
GLU
A
184
100.249
88.907
21.120
1.00
40.45
A


ATOM
1429
C
GLU
A
184
97.204
90.578
24.992
1.00
26.03
A


ATOM
1430
O
GLU
A
184
98.004
91.471
25.273
1.00
29.51
A


ATOM
1431
N
ALA
A
185
95.981
90.835
24.548
1.00
28.37
A


ATOM
1432
CA
ALA
A
185
95.560
92.236
24.317
1.00
32.04
A


ATOM
1433
CB
ALA
A
185
94.166
92.282
23.689
1.00
31.81
A


ATOM
1434
C
ALA
A
185
95.567
93.137
25.537
1.00
36.13
A


ATOM
1435
O
ALA
A
185
95.706
94.353
25.401
1.00
35.39
A


ATOM
1436
N
ASN
A
186
95.448
92.555
26.730
1.00
32.84
A


ATOM
1437
CA
ASN
A
186
95.352
93.351
27.953
1.00
29.26
A


ATOM
1438
CB
ASN
A
186
94.035
93.004
28.640
1.00
27.88
A


ATOM
1439
CG
ASN
A
186
92.847
93.480
27.836
1.00
33.22
A


ATOM
1440
OD1
ASN
A
186
92.562
94.672
27.813
1.00
34.12
A


ATOM
1441
ND2
ASN
A
186
92.175
92.566
27.143
1.00
25.25
A


ATOM
1442
C
ASN
A
186
96.476
93.240
28.952
1.00
27.19
A


ATOM
1443
O
ASN
A
186
96.444
93.890
30.003
1.00
27.97
A


ATOM
1444
N
TYR
A
187
97.472
92.433
28.610
1.00
24.51
A


ATOM
1445
CA
TYR
A
187
98.612
92.176
29.486
1.00
21.48
A


ATOM
1446
CB
TYR
A
187
99.634
91.255
28.785
1.00
23.19
A


ATOM
1447
CG
TYR
A
187
100.651
90.604
29.729
1.00
27.39
A


ATOM
1448
CD1
TYR
A
187
100.258
89.596
30.637
1.00
24.03
A


ATOM
1449
CE1
TYR
A
187
101.209
88.977
31.508
1.00
28.63
A


ATOM
1450
CD2
TYR
A
187
102.004
90.982
29.712
1.00
25.92
A


ATOM
1451
CE2
TYR
A
187
102.952
90.380
30.570
1.00
28.97
A


ATOM
1452
CZ
TYR
A
187
102.551
89.379
31.465
1.00
31.32
A


ATOM
1453
OH
TYR
A
187
103.498
88.788
32.293
1.00
25.25
A


ATOM
1454
C
TYR
A
187
99.360
93.389
29.929
1.00
29.36
A


ATOM
1455
O
TYR
A
187
99.645
93.536
31.122
1.00
26.96
A


ATOM
1456
N
MET
A
188
99.721
94.240
28.956
1.00
25.41
A


ATOM
1457
CA
MET
A
188
100.526
95.417
29.260
1.00
31.50
A


ATOM
1458
CB
MET
A
188
100.794
96.215
27.981
1.00
24.85
A


ATOM
1459
CG
MET
A
188
101.699
95.478
27.011
1.00
26.87
A


ATOM
1460
SD
MET
A
188
103.239
94.935
27.730
1.00
33.34
A


ATOM
1461
CE
MET
A
188
103.886
96.512
28.315
1.00
30.68
A


ATOM
1462
C
MET
A
188
99.962
96.322
30.349
1.00
27.28
A


ATOM
1463
O
MET
A
188
100.720
96.982
31.071
1.00
26.43
A


ATOM
1464
N
ASN
A
189
98.645
96.288
30.495
1.00
25.67
A


ATOM
1465
CA
ASN
A
189
97.927
97.092
31.482
1.00
40.32
A


ATOM
1466
CB
ASN
A
189
96.499
97.323
31.000
1.00
46.12
A


ATOM
1467
CG
ASN
A
189
96.220
98.769
30.729
1.00
58.01
A


ATOM
1468
OD1
ASN
A
189
95.564
99.113
29.728
1.00
63.96
A


ATOM
1469
ND2
ASN
A
189
96.722
99.643
31.612
1.00
54.68
A


ATOM
1470
C
ASN
A
189
97.850
96.519
32.897
1.00
40.44
A


ATOM
1471
O
ASN
A
189
97.220
97.109
33.770
1.00
40.07
A


ATOM
1472
N
LEU
A
190
98.469
95.375
33.140
1.00
37.09
A


ATOM
1473
CA
LEU
A
190
98.391
94.795
34.473
1.00
32.05
A


ATOM
1474
CB
LEU
A
190
98.887
93.358
34.459
1.00
27.91
A


ATOM
1475
CG
LEU
A
190
98.000
92.410
33.659
1.00
25.90
A


ATOM
1476
CD1
LEU
A
190
98.707
91.036
33.591
1.00
21.96
A


ATOM
1477
CD2
LEU
A
190
96.619
92.343
34.315
1.00
13.68
A


ATOM
1478
C
LEU
A
190
99.186
95.549
35.485
1.00
32.08
A


ATOM
1479
O
LEU
A
190
100.264
96.039
35.189
1.00
34.79
A


ATOM
1480
N
GLN
A
191
98.653
95.630
36.696
1.00
24.96
A


ATOM
1481
CA
GLN
A
191
99.359
96.299
37.771
1.00
29.96
A


ATOM
1482
CB
GLN
A
191
98.443
97.330
38.429
1.00
37.43
A


ATOM
1483
CG
GLN
A
191
98.000
98.434
37.486
1.00
50.78
A


ATOM
1484
CD
GLN
A
191
99.178
99.289
37.084
1.00
58.93
A


ATOM
1485
OE1
GLN
A
191
99.756
99.994
37.923
1.00
65.09
A


ATOM
1486
NE2
GLN
A
191
99.566
99.220
35.803
1.00
65.13
A


ATOM
1487
C
GLN
A
191
99.878
95.333
38.855
1.00
32.15
A


ATOM
1488
O
GLN
A
191
100.893
95.610
39.464
1.00
28.48
A


ATOM
1489
N
ARG
A
192
99.189
94.219
39.119
1.00
25.14
A


ATOM
1490
CA
ARG
A
192
99.657
93.312
40.170
1.00
22.86
A


ATOM
1491
CB
ARG
A
192
98.489
92.473
40.703
1.00
23.64
A


ATOM
1492
CG
ARG
A
192
97.302
93.308
41.257
1.00
31.91
A


ATOM
1493
CD
ARG
A
192
96.128
92.393
41.622
1.00
35.16
A


ATOM
1494
NE
ARG
A
192
96.514
91.335
42.569
1.00
41.51
A


ATOM
1495
CZ
ARG
A
192
95.847
90.184
42.733
1.00
44.28
A


ATOM
1496
NH1
ARG
A
192
94.757
89.949
41.997
1.00
39.94
A


ATOM
1497
NH2
ARG
A
192
96.255
89.280
43.641
1.00
30.46
A


ATOM
1498
C
ARG
A
192
100.736
92.356
39.709
1.00
19.29
A


ATOM
1499
O
ARG
A
192
100.658
91.822
38.596
1.00
15.56
A


ATOM
1500
N
SER
A
193
101.735
92.114
40.557
1.00
20.51
A


ATOM
1501
CA
SER
A
193
102.769
91.138
40.196
1.00
23.99
A


ATOM
1502
CB
SER
A
193
103.853
91.066
41.250
1.00
25.82
A


ATOM
1503
OG
SER
A
193
104.634
92.236
41.177
1.00
29.45
A


ATOM
1504
C
SER
A
193
102.123
89.754
40.041
1.00
26.36
A


ATOM
1505
O
SER
A
193
102.508
88.958
39.164
1.00
26.52
A


ATOM
1506
N
TYR
A
194
101.123
89.479
40.877
1.00
27.26
A


ATOM
1507
CA
TYR
A
194
100.429
88.201
40.790
1.00
22.13
A


ATOM
1508
CB
TYR
A
194
99.303
88.108
41.823
1.00
23.41
A


ATOM
1509
CG
TYR
A
194
98.539
86.783
41.755
1.00
16.74
A


ATOM
1510
CD1
TYR
A
194
99.067
85.620
42.340
1.00
19.51
A


ATOM
1511
CE1
TYR
A
194
98.401
84.389
42.271
1.00
17.85
A


ATOM
1512
CD2
TYR
A
194
97.312
86.692
41.087
1.00
20.75
A


ATOM
1513
CE2
TYR
A
194
96.621
85.461
41.001
1.00
25.88
A


ATOM
1514
CZ
TYR
A
194
97.181
84.312
41.603
1.00
20.62
A


ATOM
1515
OH
TYR
A
194
96.522
83.107
41.540
1.00
24.87
A


ATOM
1516
C
TYR
A
194
99.843
87.959
39.398
1.00
23.17
A


ATOM
1517
O
TYR
A
194
100.123
86.938
38.775
1.00
22.83
A


ATOM
1518
N
THR
A
195
99.013
88.878
38.904
1.00
18.74
A


ATOM
1519
CA
THR
A
195
98.391
88.680
37.602
1.00
16.68
A


ATOM
1520
CB
THR
A
195
97.427
89.817
37.264
1.00
16.43
A


ATOM
1521
OG1
THR
A
195
96.657
90.130
38.428
1.00
21.65
A


ATOM
1522
CG2
THR
A
195
96.496
89.378
36.151
1.00
22.25
A


ATOM
1523
C
THR
A
195
99.437
88.593
36.498
1.00
21.37
A


ATOM
1524
O
THR
A
195
99.315
87.829
35.549
1.00
18.17
A


ATOM
1525
N
VAL
A
196
100.506
89.359
36.647
1.00
23.63
A


ATOM
1526
CA
VAL
A
196
101.531
89.334
35.639
1.00
20.64
A


ATOM
1527
CB
VAL
A
196
102.620
90.409
35.959
1.00
26.84
A


ATOM
1528
CG1
VAL
A
196
103.973
90.045
35.324
1.00
25.77
A


ATOM
1529
CG2
VAL
A
196
102.154
91.773
35.456
1.00
25.74
A


ATOM
1530
C
VAL
A
196
102.147
87.933
35.583
1.00
20.37
A


ATOM
1531
O
VAL
A
196
102.451
87.439
34.503
1.00
19.78
A


ATOM
1532
N
ALA
A
197
102.386
87.326
36.739
1.00
21.05
A


ATOM
1533
CA
ALA
A
197
103.005
85.984
36.766
1.00
24.45
A


ATOM
1534
CB
ALA
A
197
103.370
85.583
38.225
1.00
15.17
A


ATOM
1535
C
ALA
A
197
102.056
84.951
36.162
1.00
15.00
A


ATOM
1536
O
ALA
A
197
102.424
84.229
35.226
1.00
20.20
A


ATOM
1537
N
ILE
A
198
100.809
84.911
36.648
1.00
24.78
A


ATOM
1538
CA
ILE
A
198
99.887
83.889
36.144
1.00
20.75
A


ATOM
1539
CB
ILE
A
198
98.569
83.723
37.056
1.00
19.12
A


ATOM
1540
CG2
ILE
A
198
97.602
84.931
36.915
1.00
20.52
A


ATOM
1541
CG1
ILE
A
198
97.855
82.438
36.658
1.00
19.50
A


ATOM
1542
CD1
ILE
A
198
96.670
82.071
37.553
1.00
20.96
A


ATOM
1543
C
ILE
A
198
99.545
84.052
34.674
1.00
19.83
A


ATOM
1544
O
ILE
A
198
99.593
83.057
33.913
1.00
21.51
A


ATOM
1545
N
ALA
A
199
99.217
85.274
34.235
1.00
19.78
A


ATOM
1546
CA
ALA
A
199
98.921
85.448
32.814
1.00
19.70
A


ATOM
1547
CB
ALA
A
199
98.323
86.823
32.537
1.00
18.52
A


ATOM
1548
C
ALA
A
199
100.226
85.268
32.049
1.00
11.15
A


ATOM
1549
O
ALA
A
199
100.228
84.804
30.911
1.00
17.30
A


ATOM
1550
N
GLY
A
200
101.345
85.621
32.680
1.00
17.10
A


ATOM
1551
CA
GLY
A
200
102.617
85.447
32.002
1.00
19.39
A


ATOM
1552
C
GLY
A
200
102.800
83.976
31.647
1.00
21.92
A


ATOM
1553
O
GLY
A
200
103.113
83.615
30.486
1.00
19.30
A


ATOM
1554
N
TYR
A
201
102.578
83.097
32.628
1.00
17.66
A


ATOM
1555
CA
TYR
A
201
102.742
81.670
32.329
1.00
23.38
A


ATOM
1556
CB
TYR
A
201
102.550
80.804
33.587
1.00
17.53
A


ATOM
1557
CG
TYR
A
201
102.641
79.303
33.313
1.00
19.35
A


ATOM
1558
CD1
TYR
A
201
103.714
78.768
32.608
1.00
20.15
A


ATOM
1559
CE1
TYR
A
201
103.815
77.383
32.384
1.00
25.42
A


ATOM
1560
CD2
TYR
A
201
101.663
78.420
33.788
1.00
26.23
A


ATOM
1561
CE2
TYR
A
201
101.749
77.037
33.573
1.00
17.93
A


ATOM
1562
CZ
TYR
A
201
102.816
76.528
32.883
1.00
25.37
A


ATOM
1563
OH
TYR
A
201
102.908
75.171
32.690
1.00
24.20
A


ATOM
1564
C
TYR
A
201
101.740
81.238
31.242
1.00
23.90
A


ATOM
1565
O
TYR
A
201
102.098
80.503
30.312
1.00
18.43
A


ATOM
1566
N
ALA
A
202
100.490
81.698
31.349
1.00
19.10
A


ATOM
1567
CA
ALA
A
202
99.484
81.306
30.367
1.00
22.19
A


ATOM
1568
CB
ALA
A
202
98.132
81.940
30.715
1.00
19.48
A


ATOM
1569
C
ALA
A
202
99.916
81.720
28.963
1.00
23.29
A


ATOM
1570
O
ALA
A
202
99.730
80.969
28.004
1.00
23.99
A


ATOM
1571
N
LEU
A
203
100.487
82.919
28.832
1.00
23.24
A


ATOM
1572
CA
LEU
A
203
100.929
83.371
27.510
1.00
23.06
A


ATOM
1573
CB
LEU
A
203
101.178
84.894
27.514
1.00
24.75
A


ATOM
1574
CG
LEU
A
203
99.907
85.744
27.664
1.00
23.34
A


ATOM
1575
CD1
LEU
A
203
100.306
87.220
27.773
1.00
27.91
A


ATOM
1576
CD2
LEU
A
203
98.963
85.548
26.489
1.00
21.31
A


ATOM
1577
C
LEU
A
203
102.182
82.611
27.060
1.00
20.01
A


ATOM
1578
O
LEU
A
203
102.321
82.260
25.892
1.00
22.51
A


ATOM
1579
N
ALA
A
204
103.087
82.325
27.985
1.00
20.31
A


ATOM
1580
CA
ALA
A
204
104.291
81.588
27.615
1.00
19.81
A


ATOM
1581
CB
ALA
A
204
105.193
81.391
28.819
1.00
16.54
A


ATOM
1582
C
ALA
A
204
103.893
80.248
27.035
1.00
22.33
A


ATOM
1583
O
ALA
A
204
104.477
79.812
26.060
1.00
23.53
A


ATOM
1584
N
GLN
A
205
102.881
79.588
27.607
1.00
21.05
A


ATOM
1585
CA
GLN
A
205
102.483
78.289
27.058
1.00
21.95
A


ATOM
1586
CB
GLN
A
205
101.309
77.717
27.840
1.00
18.19
A


ATOM
1587
CG
GLN
A
205
101.588
77.415
29.314
1.00
25.79
A


ATOM
1588
CD
GLN
A
205
100.431
76.681
29.929
1.00
29.43
A


ATOM
1589
OE1
GLN
A
205
99.299
77.200
29.967
1.00
23.15
A


ATOM
1590
NE2
GLN
A
205
100.685
75.451
30.399
1.00
23.34
A


ATOM
1591
C
GLN
A
205
102.068
78.366
25.586
1.00
27.79
A


ATOM
1592
O
GLN
A
205
102.165
77.392
24.847
1.00
24.19
A


ATOM
1593
N
MET
A
206
101.575
79.525
25.164
1.00
26.44
A


ATOM
1594
CA
MET
A
206
101.117
79.661
23.789
1.00
29.37
A


ATOM
1595
CB
MET
A
206
99.802
80.452
23.771
1.00
26.17
A


ATOM
1596
CG
MET
A
206
98.806
80.012
24.828
1.00
30.40
A


ATOM
1597
SD
MET
A
206
97.200
80.847
24.718
1.00
29.96
A


ATOM
1598
CE
MET
A
206
97.598
82.319
25.513
1.00
41.60
A


ATOM
1599
C
MET
A
206
102.138
80.349
22.889
1.00
29.92
A


ATOM
1600
O
MET
A
206
101.828
80.674
21.749
1.00
26.65
A


ATOM
1601
N
GLY
A
207
103.345
80.586
23.394
1.00
29.43
A


ATOM
1602
CA
GLY
A
207
104.352
81.278
22.593
1.00
26.83
A


ATOM
1603
C
GLY
A
207
104.014
82.753
22.378
1.00
34.90
A


ATOM
1604
O
GLY
A
207
104.534
83.393
21.464
1.00
27.87
A


ATOM
1605
N
ARG
A
208
103.171
83.313
23.244
1.00
27.86
A


ATOM
1606
CA
ARG
A
208
102.784
84.698
23.107
1.00
34.19
A


ATOM
1607
CB
ARG
A
208
101.256
84.788
23.147
1.00
33.23
A


ATOM
1608
CG
ARG
A
208
100.654
84.008
21.992
1.00
38.52
A


ATOM
1609
CD
ARG
A
208
99.191
83.574
22.162
1.00
42.22
A


ATOM
1610
NE
ARG
A
208
98.288
84.690
21.974
1.00
49.84
A


ATOM
1611
CZ
ARG
A
208
97.110
84.638
21.362
1.00
42.43
A


ATOM
1612
NH1
ARG
A
208
96.641
83.509
20.856
1.00
38.92
A


ATOM
1613
NH2
ARG
A
208
96.416
85.753
21.233
1.00
48.94
A


ATOM
1614
C
ARG
A
208
103.430
85.669
24.080
1.00
32.61
A


ATOM
1615
O
ARG
A
208
103.113
86.839
24.056
1.00
31.15
A


ATOM
1616
N
LEU
A
209
104.333
85.197
24.934
1.00
26.57
A


ATOM
1617
CA
LEU
A
209
104.990
86.093
25.879
1.00
31.14
A


ATOM
1618
CB
LEU
A
209
105.271
85.369
27.211
1.00
22.61
A


ATOM
1619
CG
LEU
A
209
105.798
86.228
28.366
1.00
35.65
A


ATOM
1620
CD1
LEU
A
209
104.766
87.332
28.723
1.00
28.29
A


ATOM
1621
CD2
LEU
A
209
106.083
85.364
29.589
1.00
28.03
A


ATOM
1622
C
LEU
A
209
106.285
86.516
25.178
1.00
32.66
A


ATOM
1623
O
LEU
A
209
107.305
85.851
25.289
1.00
32.28
A


ATOM
1624
N
LYS
A
210
106.212
87.611
24.426
1.00
32.19
A


ATOM
1625
CA
LYS
A
210
107.337
88.134
23.658
1.00
34.10
A


ATOM
1626
CB
LYS
A
210
107.118
87.884
22.169
1.00
39.67
A


ATOM
1627
CG
LYS
A
210
106.844
86.472
21.772
1.00
49.14
A


ATOM
1628
CD
LYS
A
210
106.174
86.418
20.399
1.00
51.11
A


ATOM
1629
CE
LYS
A
210
106.928
85.462
19.474
1.00
58.79
A


ATOM
1630
NZ
LYS
A
210
106.158
85.113
18.241
1.00
57.80
A


ATOM
1631
C
LYS
A
210
107.421
89.644
23.793
1.00
35.59
A


ATOM
1632
O
LYS
A
210
106.520
90.290
24.326
1.00
30.70
A


ATOM
1633
N
GLY
A
211
108.488
90.201
23.230
1.00
37.65
A


ATOM
1634
CA
GLY
A
211
108.675
91.642
23.212
1.00
34.24
A


ATOM
1635
C
GLY
A
211
108.350
92.423
24.453
1.00
26.73
A


ATOM
1636
O
GLY
A
211
108.884
92.158
25.535
1.00
31.87
A


ATOM
1637
N
PRO
A
212
107.471
93.420
24.329
1.00
35.92
A


ATOM
1638
CD
PRO
A
212
106.750
93.851
23.117
1.00
36.64
A


ATOM
1639
CA
PRO
A
212
107.096
94.252
25.480
1.00
31.77
A


ATOM
1640
CB
PRO
A
212
106.113
95.266
24.881
1.00
34.18
A


ATOM
1641
CG
PRO
A
212
106.506
95.315
23.424
1.00
36.29
A


ATOM
1642
C
PRO
A
212
106.456
93.397
26.585
1.00
29.56
A


ATOM
1643
O
PRO
A
212
106.700
93.630
27.767
1.00
27.62
A


ATOM
1644
N
LEU
A
213
105.644
92.416
26.205
1.00
26.27
A


ATOM
1645
CA
LEU
A
213
105.036
91.569
27.232
1.00
30.09
A


ATOM
1646
CB
LEU
A
213
104.048
90.590
26.613
1.00
28.37
A


ATOM
1647
CG
LEU
A
213
102.726
91.280
26.223
1.00
31.49
A


ATOM
1648
CD1
LEU
A
213
102.970
92.306
25.101
1.00
29.76
A


ATOM
1649
CD2
LEU
A
213
101.738
90.239
25.745
1.00
29.45
A


ATOM
1650
C
LEU
A
213
106.112
90.830
28.032
1.00
22.05
A


ATOM
1651
O
LEU
A
213
106.139
90.886
29.250
1.00
25.06
A


ATOM
1652
N
LEU
A
214
107.023
90.176
27.331
1.00
29.37
A


ATOM
1653
CA
LEU
A
214
108.087
89.455
27.992
1.00
31.80
A


ATOM
1654
CB
LEU
A
214
109.030
88.849
26.952
1.00
27.44
A


ATOM
1655
CG
LEU
A
214
110.272
88.115
27.476
1.00
31.65
A


ATOM
1656
CD1
LEU
A
214
109.889
86.976
28.445
1.00
25.93
A


ATOM
1657
CD2
LEU
A
214
111.030
87.536
26.246
1.00
25.20
A


ATOM
1658
C
LEU
A
214
108.836
90.401
28.889
1.00
28.32
A


ATOM
1659
O
LEU
A
214
109.110
90.091
30.049
1.00
27.69
A


ATOM
1660
N
ASN
A
215
109.176
91.576
28.365
1.00
33.16
A


ATOM
1661
CA
ASN
A
215
109.912
92.553
29.175
1.00
30.08
A


ATOM
1662
CB
ASN
A
215
110.205
93.829
28.383
1.00
37.16
A


ATOM
1663
CG
ASN
A
215
110.980
94.815
29.205
1.00
39.99
A


ATOM
1664
OD1
ASN
A
215
112.161
94.586
29.516
1.00
38.55
A


ATOM
1665
ND2
ASN
A
215
110.311
95.895
29.629
1.00
39.72
A


ATOM
1666
C
ASN
A
215
109.189
92.951
30.453
1.00
26.60
A


ATOM
1667
O
ASN
A
215
109.789
93.055
31.522
1.00
27.99
A


ATOM
1668
N
LYS
A
216
107.891
93.204
30.346
1.00
29.96
A


ATOM
1669
CA
LYS
A
216
107.116
93.562
31.536
1.00
28.98
A


ATOM
1670
CB
LYS
A
216
105.669
93.842
31.124
1.00
27.44
A


ATOM
1671
CG
LYS
A
216
104.773
94.180
32.297
1.00
25.28
A


ATOM
1672
CD
LYS
A
216
103.384
94.434
31.799
1.00
29.65
A


ATOM
1673
CE
LYS
A
216
102.375
94.667
32.940
1.00
31.45
A


ATOM
1674
NZ
LYS
A
216
102.739
95.753
33.908
1.00
33.04
A


ATOM
1675
C
LYS
A
216
107.177
92.385
32.529
1.00
31.98
A


ATOM
1676
O
LYS
A
216
107.459
92.549
33.758
1.00
23.57
A


ATOM
1677
N
PHE
A
217
106.934
91.182
32.000
1.00
29.01
A


ATOM
1678
CA
PHE
A
217
106.984
90.002
32.862
1.00
23.20
A


ATOM
1679
CB
PHE
A
217
106.849
88.715
32.053
1.00
24.49
A


ATOM
1680
CG
PHE
A
217
107.137
87.453
32.855
1.00
30.86
A


ATOM
1681
CD1
PHE
A
217
106.184
86.923
33.729
1.00
25.74
A


ATOM
1682
CD2
PHE
A
217
108.350
86.762
32.682
1.00
33.51
A


ATOM
1683
CE1
PHE
A
217
106.418
85.718
34.410
1.00
27.69
A


ATOM
1684
CE2
PHE
A
217
108.598
85.553
33.359
1.00
30.90
A


ATOM
1685
CZ
PHE
A
217
107.620
85.029
34.228
1.00
26.68
A


ATOM
1686
C
PHE
A
217
108.277
89.949
33.655
1.00
25.85
A


ATOM
1687
O
PHE
A
217
108.255
89.888
34.892
1.00
26.90
A


ATOM
1688
N
LEU
A
218
109.417
90.010
32.967
1.00
29.26
A


ATOM
1689
CA
LEU
A
218
110.700
89.915
33.668
1.00
27.29
A


ATOM
1690
CB
LEU
A
218
111.853
89.742
32.671
1.00
29.60
A


ATOM
1691
CG
LEU
A
218
111.709
88.572
31.682
1.00
32.78
A


ATOM
1692
CD1
LEU
A
218
112.729
88.696
30.537
1.00
27.26
A


ATOM
1693
CD2
LEU
A
218
111.905
87.240
32.455
1.00
24.94
A


ATOM
1694
C
LEU
A
218
111.016
91.071
34.609
1.00
30.13
A


ATOM
1695
O
LEU
A
218
111.452
90.857
35.751
1.00
31.21
A


ATOM
1696
N
THR
A
219
110.798
92.295
34.141
1.00
31.78
A


ATOM
1697
CA
THR
A
219
111.097
93.480
34.958
1.00
35.74
A


ATOM
1698
CB
THR
A
219
111.082
94.772
34.097
1.00
33.10
A


ATOM
1699
OG1
THR
A
219
109.778
94.981
33.536
1.00
31.70
A


ATOM
1700
CG2
THR
A
219
112.106
94.645
32.947
1.00
34.85
A


ATOM
1701
C
THR
A
219
110.138
93.626
36.142
1.00
30.35
A


ATOM
1702
O
THR
A
219
110.431
94.334
37.101
1.00
30.89
A


ATOM
1703
N
THR
A
220
109.007
92.931
36.094
1.00
31.42
A


ATOM
1704
CA
THR
A
220
108.081
93.014
37.216
1.00
29.70
A


ATOM
1705
CB
THR
A
220
106.715
92.404
36.867
1.00
33.79
A


ATOM
1706
OG1
THR
A
220
106.091
93.206
35.862
1.00
29.37
A


ATOM
1707
CG2
THR
A
220
105.794
92.390
38.097
1.00
36.20
A


ATOM
1708
C
THR
A
220
108.666
92.314
38.437
1.00
30.26
A


ATOM
1709
O
THR
A
220
108.320
92.622
39.579
1.00
32.43
A


ATOM
1710
N
ALA
A
221
109.590
91.388
38.223
1.00
30.76
A


ATOM
1711
CA
ALA
A
221
110.140
90.711
39.383
1.00
33.31
A


ATOM
1712
CB
ALA
A
221
111.030
89.552
38.950
1.00
29.98
A


ATOM
1713
C
ALA
A
221
110.903
91.626
40.341
1.00
33.78
A


ATOM
1714
O
ALA
A
221
111.664
92.480
39.932
1.00
37.00
A


ATOM
1715
N
LYS
A
222
110.682
91.436
41.633
1.00
35.09
A


ATOM
1716
CA
LYS
A
222
111.385
92.202
42.645
1.00
35.18
A


ATOM
1717
CB
LYS
A
222
110.598
92.185
43.955
1.00
43.27
A


ATOM
1718
CG
LYS
A
222
111.085
93.202
44.970
1.00
51.49
A


ATOM
1719
CD
LYS
A
222
110.204
93.213
46.227
1.00
60.05
A


ATOM
1720
CE
LYS
A
222
110.402
94.508
47.040
1.00
63.10
A


ATOM
1721
NZ
LYS
A
222
111.855
94.791
47.290
1.00
63.82
A


ATOM
1722
C
LYS
A
222
112.740
91.534
42.864
1.00
39.12
A


ATOM
1723
O
LYS
A
222
112.811
90.313
43.020
1.00
31.62
A


ATOM
1724
N
ASP
A
223
113.809
92.332
42.906
1.00
35.35
A


ATOM
1725
CA
ASP
A
223
115.168
91.818
43.097
1.00
33.61
A


ATOM
1726
CB
ASP
A
223
115.383
91.275
44.518
1.00
42.55
A


ATOM
1727
CG
ASP
A
223
115.290
92.349
45.583
1.00
50.58
A


ATOM
1728
OD1
ASP
A
223
114.908
92.022
46.733
1.00
53.29
A


ATOM
1729
OD2
ASP
A
223
115.609
93.518
45.282
1.00
58.47
A


ATOM
1730
C
ASP
A
223
115.448
90.696
42.115
1.00
31.36
A


ATOM
1731
O
ASP
A
223
116.247
89.804
42.408
1.00
33.77
A


ATOM
1732
N
LYS
A
224
114.786
90.737
40.962
1.00
30.77
A


ATOM
1733
CA
LYS
A
224
114.964
89.706
39.940
1.00
35.01
A


ATOM
1734
CB
LYS
A
224
116.370
89.807
39.382
1.00
36.58
A


ATOM
1735
CG
LYS
A
224
116.683
91.208
38.878
1.00
47.96
A


ATOM
1736
CD
LYS
A
224
118.037
91.262
38.207
1.00
52.39
A


ATOM
1737
CE
LYS
A
224
118.284
92.622
37.573
1.00
56.76
A


ATOM
1738
NZ
LYS
A
224
119.429
92.538
36.606
1.00
59.77
A


ATOM
1739
C
LYS
A
224
114.720
88.268
40.446
1.00
35.05
A


ATOM
1740
O
LYS
A
224
115.169
87.296
39.832
1.00
31.24
A


ATOM
1741
N
ASN
A
225
114.026
88.117
41.566
1.00
27.53
A


ATOM
1742
CA
ASN
A
225
113.811
86.756
42.053
1.00
33.64
A


ATOM
1743
CB
ASN
A
225
114.834
86.408
43.156
1.00
24.93
A


ATOM
1744
CG
ASN
A
225
114.614
87.203
44.456
1.00
32.88
A


ATOM
1745
OD1
ASN
A
225
115.462
87.178
45.355
1.00
27.17
A


ATOM
1746
ND2
ASN
A
225
113.477
87.896
44.563
1.00
23.33
A


ATOM
1747
C
ASN
A
225
112.391
86.412
42.506
1.00
32.97
A


ATOM
1748
O
ASN
A
225
112.114
85.247
42.781
1.00
29.39
A


ATOM
1749
N
ARG
A
226
111.481
87.385
42.587
1.00
27.45
A


ATOM
1750
CA
ARG
A
226
110.126
87.028
43.007
1.00
24.16
A


ATOM
1751
CB
ARG
A
226
110.055
86.996
44.528
1.00
29.81
A


ATOM
1752
CG
ARG
A
226
110.358
88.346
45.221
1.00
29.04
A


ATOM
1753
CD
ARG
A
226
110.419
88.133
46.717
1.00
29.72
A


ATOM
1754
NE
ARG
A
226
110.694
89.338
47.493
1.00
36.06
A


ATOM
1755
CZ
ARG
A
226
111.862
89.992
47.530
1.00
40.98
A


ATOM
1756
NH1
ARG
A
226
112.909
89.572
46.818
1.00
42.69
A


ATOM
1757
NH2
ARG
A
226
111.994
91.066
48.310
1.00
37.88
A


ATOM
1758
C
ARG
A
226
109.069
87.973
42.477
1.00
31.57
A


ATOM
1759
O
ARG
A
226
109.330
89.143
42.211
1.00
25.27
A


ATOM
1760
N
TRP
A
227
107.867
87.456
42.299
1.00
28.37
A


ATOM
1761
CA
TRP
A
227
106.761
88.275
41.851
1.00
23.41
A


ATOM
1762
CB
TRP
A
227
105.973
87.536
40.760
1.00
20.74
A


ATOM
1763
CG
TRP
A
227
106.620
87.650
39.384
1.00
26.80
A


ATOM
1764
CD2
TRP
A
227
107.850
87.041
38.950
1.00
20.68
A


ATOM
1765
CE2
TRP
A
227
108.105
87.500
37.630
1.00
22.35
A


ATOM
1766
CE3
TRP
A
227
108.763
86.157
39.549
1.00
22.82
A


ATOM
1767
CD1
TRP
A
227
106.189
88.430
38.330
1.00
21.29
A


ATOM
1768
NE1
TRP
A
227
107.079
88.344
37.281
1.00
28.98
A


ATOM
1769
CZ2
TRP
A
227
109.225
87.105
36.897
1.00
24.84
A


ATOM
1770
CZ3
TRP
A
227
109.886
85.757
38.821
1.00
22.71
A


ATOM
1771
CH2
TRP
A
227
110.105
86.232
37.505
1.00
26.46
A


ATOM
1772
C
TRP
A
227
105.918
88.521
43.097
1.00
31.42
A


ATOM
1773
O
TRP
A
227
105.162
87.658
43.531
1.00
30.37
A


ATOM
1774
N
GLU
A
228
106.081
89.674
43.723
1.00
30.19
A


ATOM
1775
CA
GLU
A
228
105.278
89.914
44.897
1.00
40.90
A


ATOM
1776
CB
GLU
A
228
106.105
89.747
46.169
1.00
43.17
A


ATOM
1777
CG
GLU
A
228
107.143
90.767
46.430
1.00
49.83
A


ATOM
1778
CD
GLU
A
228
107.521
90.796
47.904
1.00
51.45
A


ATOM
1779
OE1
GLU
A
228
107.955
89.753
48.428
1.00
41.85
A


ATOM
1780
OE2
GLU
A
228
107.375
91.865
48.534
1.00
56.43
A


ATOM
1781
C
GLU
A
228
104.555
91.248
44.907
1.00
43.90
A


ATOM
1782
O
GLU
A
228
105.015
92.225
44.320
1.00
38.10
A


ATOM
1783
N
ASP
A
229
103.398
91.261
45.559
1.00
45.53
A


ATOM
1784
CA
ASP
A
229
102.606
92.462
45.661
1.00
49.47
A


ATOM
1785
CB
ASP
A
229
101.178
92.149
45.244
1.00
48.06
A


ATOM
1786
CG
ASP
A
229
101.057
91.972
43.727
1.00
50.49
A


ATOM
1787
OD1
ASP
A
229
101.471
92.917
43.003
1.00
49.52
A


ATOM
1788
OD2
ASP
A
229
100.573
90.910
43.263
1.00
34.83
A


ATOM
1789
C
ASP
A
229
102.716
92.944
47.097
1.00
56.94
A


ATOM
1790
O
ASP
A
229
102.511
92.165
48.033
1.00
47.42
A


ATOM
1791
N
PRO
A
230
103.104
94.229
47.290
1.00
64.41
A


ATOM
1792
CD
PRO
A
230
103.414
95.217
46.238
1.00
66.97
A


ATOM
1793
CA
PRO
A
230
103.261
94.830
48.625
1.00
64.83
A


ATOM
1794
CB
PRO
A
230
103.783
96.239
48.322
1.00
68.61
A


ATOM
1795
CG
PRO
A
230
103.206
96.530
46.968
1.00
71.34
A


ATOM
1796
C
PRO
A
230
101.958
94.835
49.400
1.00
61.61
A


ATOM
1797
O
PRO
A
230
100.886
94.963
48.819
1.00
59.14
A


ATOM
1798
N
GLY
A
231
102.066
94.671
50.713
1.00
64.01
A


ATOM
1799
CA
GLY
A
231
100.884
94.647
51.552
1.00
69.86
A


ATOM
1800
C
GLY
A
231
100.197
93.305
51.447
1.00
70.74
A


ATOM
1801
O
GLY
A
231
99.407
93.085
50.526
1.00
71.02
A


ATOM
1802
N
LYS
A
232
100.487
92.408
52.388
1.00
74.07
A


ATOM
1803
CA
LYS
A
232
99.899
91.068
52.345
1.00
77.87
A


ATOM
1804
CB
LYS
A
232
98.369
91.170
52.297
1.00
80.70
A


ATOM
1805
CG
LYS
A
232
97.722
91.539
53.624
1.00
83.42
A


ATOM
1806
CD
LYS
A
232
97.830
90.372
54.609
1.00
85.26
A


ATOM
1807
CE
LYS
A
232
97.028
90.622
55.884
1.00
85.21
A


ATOM
1808
NZ
LYS
A
232
97.018
89.416
56.769
1.00
86.74
A


ATOM
1809
C
LYS
A
232
100.443
90.345
51.087
1.00
77.00
A


ATOM
1810
O
LYS
A
232
99.701
89.982
50.156
1.00
76.48
A


ATOM
1811
N
GLN
A
233
101.762
90.168
51.071
1.00
70.42
A


ATOM
1812
CA
GLN
A
233
102.447
89.508
49.971
1.00
64.94
A


ATOM
1813
CB
GLN
A
233
103.895
89.999
49.916
1.00
65.15
A


ATOM
1814
CG
GLN
A
233
104.660
89.787
51.211
1.00
68.22
A


ATOM
1815
CD
GLN
A
233
105.334
91.059
51.716
1.00
73.51
A


ATOM
1816
OE1
GLN
A
233
106.256
91.581
51.087
1.00
79.17
A


ATOM
1817
NE2
GLN
A
233
104.869
91.567
52.859
1.00
73.37
A


ATOM
1818
C
GLN
A
233
102.416
88.003
50.192
1.00
62.45
A


ATOM
1819
O
GLN
A
233
103.423
87.332
50.007
1.00
59.36
A


ATOM
1820
N
LEU
A
234
101.267
87.473
50.604
1.00
59.16
A


ATOM
1821
CA
LEU
A
234
101.163
86.040
50.844
1.00
57.14
A


ATOM
1822
CB
LEU
A
234
100.011
85.733
51.808
1.00
58.67
A


ATOM
1823
CG
LEU
A
234
98.593
86.146
51.406
1.00
59.33
A


ATOM
1824
CD1
LEU
A
234
97.566
85.419
52.300
1.00
55.69
A


ATOM
1825
CD2
LEU
A
234
98.455
87.680
51.521
1.00
56.83
A


ATOM
1826
C
LEU
A
234
101.004
85.222
49.548
1.00
56.11
A


ATOM
1827
O
LEU
A
234
101.067
83.977
49.572
1.00
58.17
A


ATOM
1828
N
TYR
A
235
100.835
85.906
48.415
1.00
43.77
A


ATOM
1829
CA
TYR
A
235
100.694
85.205
47.142
1.00
33.72
A


ATOM
1830
CB
TYR
A
235
99.701
85.954
46.262
1.00
29.90
A


ATOM
1831
CG
TYR
A
235
98.416
86.253
46.982
1.00
31.05
A


ATOM
1832
CD1
TYR
A
235
97.805
87.505
46.872
1.00
32.37
A


ATOM
1833
CE1
TYR
A
235
96.614
87.785
47.530
1.00
27.48
A


ATOM
1834
CD2
TYR
A
235
97.799
85.273
47.779
1.00
29.78
A


ATOM
1835
CE2
TYR
A
235
96.615
85.533
48.437
1.00
27.57
A


ATOM
1836
CZ
TYR
A
235
96.026
86.789
48.303
1.00
33.50
A


ATOM
1837
OH
TYR
A
235
94.822
87.009
48.889
1.00
30.21
A


ATOM
1838
C
TYR
A
235
102.015
85.112
46.401
1.00
31.37
A


ATOM
1839
O
TYR
A
235
102.027
84.671
45.262
1.00
28.36
A


ATOM
1840
N
ASN
A
236
103.130
85.519
47.023
1.00
24.27
A


ATOM
1841
CA
ASN
A
236
104.399
85.505
46.292
1.00
20.44
A


ATOM
1842
CB
ASN
A
236
105.472
86.335
47.017
1.00
25.88
A


ATOM
1843
CG
ASN
A
236
105.651
85.944
48.484
1.00
30.25
A


ATOM
1844
OD1
ASN
A
236
104.890
85.148
49.032
1.00
39.82
A


ATOM
1845
ND2
ASN
A
236
106.644
86.543
49.133
1.00
28.85
A


ATOM
1846
C
ASN
A
236
104.955
84.137
45.960
1.00
20.82
A


ATOM
1847
O
ASN
A
236
105.588
83.968
44.919
1.00
20.31
A


ATOM
1848
N
VAL
A
237
104.761
83.155
46.831
1.00
20.09
A


ATOM
1849
CA
VAL
A
237
105.272
81.827
46.501
1.00
21.36
A


ATOM
1850
CB
VAL
A
237
105.154
80.861
47.704
1.00
18.71
A


ATOM
1851
CG1
VAL
A
237
105.601
79.471
47.312
1.00
14.08
A


ATOM
1852
CG2
VAL
A
237
106.052
81.376
48.846
1.00
27.50
A


ATOM
1853
C
VAL
A
237
104.481
81.299
45.301
1.00
18.56
A


ATOM
1854
O
VAL
A
237
105.063
80.794
44.343
1.00
18.22
A


ATOM
1855
N
GLU
A
238
103.162
81.479
45.323
1.00
17.79
A


ATOM
1856
CA
GLU
A
238
102.315
80.996
44.239
1.00
16.12
A


ATOM
1857
CB
GLU
A
238
100.824
81.147
44.601
1.00
17.19
A


ATOM
1858
CG
GLU
A
238
99.867
80.782
43.468
1.00
14.34
A


ATOM
1859
CD
GLU
A
238
98.471
81.420
43.650
1.00
11.06
A


ATOM
1860
OE1
GLU
A
238
98.241
82.107
44.686
1.00
19.48
A


ATOM
1861
OE2
GLU
A
238
97.621
81.200
42.783
1.00
16.80
A


ATOM
1862
C
GLU
A
238
102.628
81.744
42.958
1.00
20.25
A


ATOM
1863
O
GLU
A
238
102.845
81.130
41.897
1.00
18.11
A


ATOM
1864
N
ALA
A
239
102.684
83.071
43.036
1.00
18.43
A


ATOM
1865
CA
ALA
A
239
102.975
83.867
41.835
1.00
17.27
A


ATOM
1866
CB
ALA
A
239
102.988
85.340
42.221
1.00
17.99
A


ATOM
1867
C
ALA
A
239
104.334
83.488
41.251
1.00
16.91
A


ATOM
1868
O
ALA
A
239
104.510
83.248
40.038
1.00
18.31
A


ATOM
1869
N
THR
A
240
105.312
83.398
42.136
1.00
12.73
A


ATOM
1870
CA
THR
A
240
106.678
83.101
41.673
1.00
16.78
A


ATOM
1871
CB
THR
A
240
107.675
83.337
42.835
1.00
20.26
A


ATOM
1872
OG1
THR
A
240
107.499
84.683
43.322
1.00
20.23
A


ATOM
1873
CG2
THR
A
240
109.110
83.187
42.382
1.00
15.53
A


ATOM
1874
C
THR
A
240
106.790
81.686
41.087
1.00
23.59
A


ATOM
1875
O
THR
A
240
107.639
81.437
40.215
1.00
20.02
A


ATOM
1876
N
SER
A
241
105.941
80.760
41.552
1.00
15.58
A


ATOM
1877
CA
SER
A
241
105.991
79.381
41.022
1.00
17.43
A


ATOM
1878
CB
SER
A
241
105.162
78.441
41.906
1.00
11.67
A


ATOM
1879
OG
SER
A
241
105.715
78.363
43.233
1.00
15.93
A


ATOM
1880
C
SER
A
241
105.438
79.442
39.595
1.00
17.10
A


ATOM
1881
O
SER
A
241
105.980
78.810
38.675
1.00
18.31
A


ATOM
1882
N
TYR
A
242
104.365
80.217
39.383
1.00
18.46
A


ATOM
1883
CA
TYR
A
242
103.846
80.367
38.013
1.00
18.91
A


ATOM
1884
CB
TYR
A
242
102.622
81.307
37.942
1.00
15.98
A


ATOM
1885
CG
TYR
A
242
101.263
80.652
38.192
1.00
20.69
A


ATOM
1886
CD1
TYR
A
242
100.766
79.658
37.338
1.00
13.29
A


ATOM
1887
CE1
TYR
A
242
99.504
79.043
37.567
1.00
18.13
A


ATOM
1888
CD2
TYR
A
242
100.486
81.030
39.286
1.00
16.91
A


ATOM
1889
CE2
TYR
A
242
99.246
80.434
39.537
1.00
11.03
A


ATOM
1890
CZ
TYR
A
242
98.759
79.444
38.669
1.00
19.17
A


ATOM
1891
OH
TYR
A
242
97.505
78.916
38.898
1.00
17.62
A


ATOM
1892
C
TYR
A
242
104.949
81.000
37.157
1.00
23.33
A


ATOM
1893
O
TYR
A
242
105.190
80.603
35.997
1.00
22.29
A


ATOM
1894
N
ALA
A
243
105.613
82.004
37.717
1.00
18.34
A


ATOM
1895
CA
ALA
A
243
106.634
82.697
36.929
1.00
18.89
A


ATOM
1896
CB
ALA
A
243
107.164
83.901
37.694
1.00
19.57
A


ATOM
1897
C
ALA
A
243
107.772
81.717
36.608
1.00
21.61
A


ATOM
1898
O
ALA
A
243
108.290
81.731
35.494
1.00
21.45
A


ATOM
1899
N
LEU
A
244
108.145
80.861
37.565
1.00
16.89
A


ATOM
1900
CA
LEU
A
244
109.239
79.899
37.309
1.00
20.89
A


ATOM
1901
CB
LEU
A
244
109.588
79.103
38.587
1.00
16.59
A


ATOM
1902
CG
LEU
A
244
110.578
77.933
38.379
1.00
21.10
A


ATOM
1903
CD1
LEU
A
244
111.844
78.449
37.632
1.00
20.05
A


ATOM
1904
CD2
LEU
A
244
110.974
77.326
39.777
1.00
12.73
A


ATOM
1905
C
LEU
A
244
108.813
78.947
36.182
1.00
18.67
A


ATOM
1906
O
LEU
A
244
109.610
78.556
35.296
1.00
19.02
A


ATOM
1907
N
LEU
A
245
107.542
78.574
36.181
1.00
17.68
A


ATOM
1908
CA
LEU
A
245
107.074
77.709
35.107
1.00
15.21
A


ATOM
1909
CB
LEU
A
245
105.628
77.214
35.404
1.00
20.70
A


ATOM
1910
CG
LEU
A
245
105.557
76.123
36.506
1.00
21.76
A


ATOM
1911
CD1
LEU
A
245
104.108
75.956
36.996
1.00
26.34
A


ATOM
1912
CD2
LEU
A
245
106.100
74.778
35.969
1.00
16.28
A


ATOM
1913
C
LEU
A
245
107.147
78.449
33.746
1.00
19.30
A


ATOM
1914
O
LEU
A
245
107.449
77.831
32.717
1.00
20.79
A


ATOM
1915
N
ALA
A
246
106.874
79.759
33.724
1.00
18.12
A


ATOM
1916
CA
ALA
A
246
106.959
80.501
32.461
1.00
18.77
A


ATOM
1917
CB
ALA
A
246
106.367
81.944
32.622
1.00
16.45
A


ATOM
1918
C
ALA
A
246
108.431
80.558
32.009
1.00
14.13
A


ATOM
1919
O
ALA
A
246
108.738
80.352
30.823
1.00
19.75
A


ATOM
1920
N
LEU
A
247
109.337
80.835
32.944
1.00
15.08
A


ATOM
1921
CA
LEU
A
247
110.769
80.899
32.620
1.00
18.36
A


ATOM
1922
CB
LEU
A
247
111.567
81.267
33.866
1.00
19.66
A


ATOM
1923
CG
LEU
A
247
111.365
82.715
34.363
1.00
20.28
A


ATOM
1924
CD1
LEU
A
247
111.874
82.820
35.813
1.00
17.02
A


ATOM
1925
CD2
LEU
A
247
112.098
83.725
33.441
1.00
21.44
A


ATOM
1926
C
LEU
A
247
111.263
79.557
32.058
1.00
23.09
A


ATOM
1927
O
LEU
A
247
112.038
79.526
31.092
1.00
25.65
A


ATOM
1928
N
LEU
A
248
110.796
78.447
32.624
1.00
22.19
A


ATOM
1929
CA
LEU
A
248
111.239
77.124
32.123
1.00
28.05
A


ATOM
1930
CB
LEU
A
248
110.815
75.977
33.081
1.00
24.02
A


ATOM
1931
CG
LEU
A
248
111.577
75.998
34.437
1.00
25.05
A


ATOM
1932
CD1
LEU
A
248
110.943
75.071
35.436
1.00
24.06
A


ATOM
1933
CD2
LEU
A
248
113.066
75.617
34.216
1.00
15.01
A


ATOM
1934
C
LEU
A
248
110.657
76.914
30.745
1.00
23.21
A


ATOM
1935
O
LEU
A
248
111.313
76.391
29.847
1.00
25.42
A


ATOM
1936
N
GLN
A
249
109.416
77.324
30.572
1.00
22.82
A


ATOM
1937
CA
GLN
A
249
108.757
77.197
29.281
1.00
22.86
A


ATOM
1938
CB
GLN
A
249
107.314
77.694
29.377
1.00
27.58
A


ATOM
1939
CG
GLN
A
249
106.526
77.545
28.085
1.00
34.66
A


ATOM
1940
CD
GLN
A
249
106.170
76.085
27.789
1.00
39.67
A


ATOM
1941
OE1
GLN
A
249
105.459
75.434
28.554
1.00
44.37
A


ATOM
1942
NE2
GLN
A
249
106.672
75.575
26.687
1.00
37.71
A


ATOM
1943
C
GLN
A
249
109.513
78.042
28.226
1.00
27.62
A


ATOM
1944
O
GLN
A
249
109.623
77.655
27.079
1.00
24.60
A


ATOM
1945
N
LEU
A
250
110.009
79.207
28.618
1.00
32.08
A


ATOM
1946
CA
LEU
A
250
110.740
80.080
27.700
1.00
30.59
A


ATOM
1947
CB
LEU
A
250
110.736
81.501
28.247
1.00
28.66
A


ATOM
1948
CG
LEU
A
250
109.401
82.220
28.410
1.00
30.11
A


ATOM
1949
CD1
LEU
A
250
109.530
83.326
29.426
1.00
29.85
A


ATOM
1950
CD2
LEU
A
250
108.960
82.728
27.062
1.00
28.49
A


ATOM
1951
C
LEU
A
250
112.207
79.647
27.551
1.00
36.31
A


ATOM
1952
O
LEU
A
250
112.938
80.189
26.708
1.00
31.07
A


ATOM
1953
N
LYS
A
251
112.627
78.682
28.373
1.00
30.98
A


ATOM
1954
CA
LYS
A
251
114.016
78.226
28.410
1.00
31.57
A


ATOM
1955
CB
LYS
A
251
114.420
77.554
27.098
1.00
32.85
A


ATOM
1956
CG
LYS
A
251
113.715
76.232
26.890
1.00
42.53
A


ATOM
1957
CD
LYS
A
251
114.060
75.631
25.555
1.00
46.71
A


ATOM
1958
CE
LYS
A
251
113.233
74.376
25.314
1.00
54.63
A


ATOM
1959
NZ
LYS
A
251
113.214
73.987
23.864
1.00
58.25
A


ATOM
1960
C
LYS
A
251
114.943
79.412
28.712
1.00
31.12
A


ATOM
1961
O
LYS
A
251
116.102
79.442
28.301
1.00
32.07
A


ATOM
1962
N
ASP
A
252
114.438
80.376
29.470
1.00
29.36
A


ATOM
1963
CA
ASP
A
252
115.239
81.521
29.830
1.00
28.62
A


ATOM
1964
CB
ASP
A
252
114.346
82.738
30.091
1.00
33.61
A


ATOM
1965
CG
ASP
A
252
115.150
84.017
30.237
1.00
37.36
A


ATOM
1966
OD1
ASP
A
252
116.328
83.990
29.839
1.00
49.12
A


ATOM
1967
OD2
ASP
A
252
114.624
85.048
30.725
1.00
34.68
A


ATOM
1968
C
ASP
A
252
116.097
81.183
31.046
1.00
31.99
A


ATOM
1969
O
ASP
A
252
115.933
81.742
32.139
1.00
30.76
A


ATOM
1970
N
PHE
A
253
117.069
80.301
30.819
1.00
31.40
A


ATOM
1971
CA
PHE
A
253
117.935
79.818
31.873
1.00
27.03
A


ATOM
1972
CB
PHE
A
253
118.786
78.674
31.338
1.00
33.08
A


ATOM
1973
CG
PHE
A
253
117.995
77.601
30.649
1.00
33.23
A


ATOM
1974
CD1
PHE
A
253
116.815
77.109
31.205
1.00
35.86
A


ATOM
1975
CD2
PHE
A
253
118.460
77.032
29.463
1.00
34.30
A


ATOM
1976
CE1
PHE
A
253
116.115
76.061
30.593
1.00
35.38
A


ATOM
1977
CE2
PHE
A
253
117.762
75.987
28.853
1.00
30.02
A


ATOM
1978
CZ
PHE
A
253
116.593
75.504
29.421
1.00
32.95
A


ATOM
1979
C
PHE
A
253
118.823
80.800
32.619
1.00
36.01
A


ATOM
1980
O
PHE
A
253
119.249
80.512
33.746
1.00
32.40
A


ATOM
1981
N
ASP
A
254
119.124
81.951
32.028
1.00
33.64
A


ATOM
1982
CA
ASP
A
254
119.986
82.905
32.728
1.00
37.81
A


ATOM
1983
CB
ASP
A
254
120.588
83.884
31.729
1.00
49.35
A


ATOM
1984
CG
ASP
A
254
119.539
84.520
30.871
1.00
65.34
A


ATOM
1985
OD1
ASP
A
254
118.936
83.793
30.046
1.00
75.38
A


ATOM
1986
OD2
ASP
A
254
119.293
85.740
31.023
1.00
75.70
A


ATOM
1987
C
ASP
A
254
119.210
83.669
33.802
1.00
31.65
A


ATOM
1988
O
ASP
A
254
119.791
84.196
34.752
1.00
30.63
A


ATOM
1989
N
PHE
A
255
117.892
83.728
33.652
1.00
28.90
A


ATOM
1990
CA
PHE
A
255
117.058
84.442
34.616
1.00
29.25
A


ATOM
1991
CB
PHE
A
255
115.881
85.100
33.892
1.00
28.92
A


ATOM
1992
CG
PHE
A
255
115.234
86.213
34.671
1.00
38.13
A


ATOM
1993
CD1
PHE
A
255
114.281
85.947
35.643
1.00
31.13
A


ATOM
1994
CD2
PHE
A
255
115.596
87.546
34.432
1.00
39.51
A


ATOM
1995
CE1
PHE
A
255
113.695
86.988
36.366
1.00
38.06
A


ATOM
1996
CE2
PHE
A
255
115.017
88.589
35.150
1.00
35.88
A


ATOM
1997
CZ
PHE
A
255
114.063
88.313
36.120
1.00
39.10
A


ATOM
1998
C
PHE
A
255
116.523
83.530
35.721
1.00
28.15
A


ATOM
1999
O
PHE
A
255
116.078
83.996
36.767
1.00
27.42
A


ATOM
2000
N
VAL
A
256
116.562
82.228
35.482
1.00
24.41
A


ATOM
2001
CA
VAL
A
256
116.040
81.257
36.453
1.00
21.90
A


ATOM
2002
CB
VAL
A
256
116.017
79.838
35.789
1.00
26.19
A


ATOM
2003
CG1
VAL
A
256
115.894
78.725
36.829
1.00
17.67
A


ATOM
2004
CG2
VAL
A
256
114.827
79.770
34.812
1.00
22.75
A


ATOM
2005
C
VAL
A
256
116.666
81.130
37.848
1.00
22.10
A


ATOM
2006
O
VAL
A
256
115.946
81.095
38.871
1.00
28.02
A


ATOM
2007
N
PRO
A
257
118.007
81.124
37.931
1.00
21.20
A


ATOM
2008
CD
PRO
A
257
118.995
81.442
36.886
1.00
24.71
A


ATOM
2009
CA
PRO
A
257
118.667
80.963
39.234
1.00
24.35
A


ATOM
2010
CB
PRO
A
257
120.169
81.097
38.899
1.00
27.97
A


ATOM
2011
CG
PRO
A
257
120.234
80.711
37.411
1.00
23.95
A


ATOM
2012
C
PRO
A
257
118.258
81.802
40.403
1.00
24.79
A


ATOM
2013
O
PRO
A
257
117.988
81.266
41.468
1.00
27.34
A


ATOM
2014
N
PRO
A
258
118.189
83.134
40.250
1.00
25.57
A


ATOM
2015
CD
PRO
A
258
118.491
84.076
39.165
1.00
26.97
A


ATOM
2016
CA
PRO
A
258
117.785
83.828
41.487
1.00
25.28
A


ATOM
2017
CB
PRO
A
258
117.840
85.322
41.103
1.00
29.92
A


ATOM
2018
CG
PRO
A
258
118.862
85.363
39.944
1.00
31.32
A


ATOM
2019
C
PRO
A
258
116.373
83.414
41.927
1.00
25.27
A


ATOM
2020
O
PRO
A
258
116.032
83.438
43.117
1.00
21.56
A


ATOM
2021
N
VAL
A
259
115.552
83.013
40.963
1.00
21.83
A


ATOM
2022
CA
VAL
A
259
114.175
82.658
41.291
1.00
22.97
A


ATOM
2023
CB
VAL
A
259
113.339
82.571
39.988
1.00
21.99
A


ATOM
2024
CG1
VAL
A
259
111.917
82.096
40.294
1.00
17.20
A


ATOM
2025
CG2
VAL
A
259
113.271
83.961
39.346
1.00
24.31
A


ATOM
2026
C
VAL
A
259
114.069
81.358
42.085
1.00
19.11
A


ATOM
2027
O
VAL
A
259
113.366
81.256
43.091
1.00
22.76
A


ATOM
2028
N
VAL
A
260
114.783
80.357
41.620
1.00
26.91
A


ATOM
2029
CA
VAL
A
260
114.760
79.071
42.275
1.00
24.36
A


ATOM
2030
CB
VAL
A
260
115.564
78.094
41.451
1.00
23.58
A


ATOM
2031
CG1
VAL
A
260
115.723
76.787
42.189
1.00
24.55
A


ATOM
2032
CG2
VAL
A
260
114.865
77.887
40.155
1.00
21.01
A


ATOM
2033
C
VAL
A
260
115.340
79.231
43.657
1.00
24.01
A


ATOM
2034
O
VAL
A
260
114.815
78.721
44.651
1.00
20.85
A


ATOM
2035
N
ARG
A
261
116.436
79.972
43.724
1.00
24.50
A


ATOM
2036
CA
ARG
A
261
117.084
80.219
45.002
1.00
19.29
A


ATOM
2037
CB
ARG
A
261
118.320
81.101
44.757
1.00
26.10
A


ATOM
2038
CG
ARG
A
261
119.216
81.291
45.956
1.00
42.29
A


ATOM
2039
CD
ARG
A
261
120.449
82.133
45.594
1.00
48.37
A


ATOM
2040
NE
ARG
A
261
121.239
81.474
44.553
1.00
53.77
A


ATOM
2041
CZ
ARG
A
261
121.406
81.910
43.301
1.00
58.06
A


ATOM
2042
NH1
ARG
A
261
120.845
83.045
42.877
1.00
55.53
A


ATOM
2043
NH2
ARG
A
261
122.128
81.180
42.454
1.00
54.87
A


ATOM
2044
C
ARG
A
261
116.114
80.883
45.999
1.00
26.55
A


ATOM
2045
O
ARG
A
261
116.056
80.507
47.169
1.00
25.37
A


ATOM
2046
N
TRP
A
262
115.353
81.885
45.556
1.00
21.10
A


ATOM
2047
CA
TRP
A
262
114.423
82.526
46.469
1.00
21.13
A


ATOM
2048
CB
TRP
A
262
113.759
83.756
45.804
1.00
27.41
A


ATOM
2049
CG
TRP
A
262
112.747
84.440
46.710
1.00
26.23
A


ATOM
2050
CD2
TRP
A
262
111.344
84.176
46.770
1.00
24.38
A


ATOM
2051
CE2
TRP
A
262
110.809
84.949
47.839
1.00
22.85
A


ATOM
2052
CE3
TRP
A
262
110.479
83.359
46.027
1.00
29.17
A


ATOM
2053
CD1
TRP
A
262
113.008
85.351
47.715
1.00
27.15
A


ATOM
2054
NE1
TRP
A
262
111.844
85.659
48.397
1.00
25.55
A


ATOM
2055
CZ2
TRP
A
262
109.458
84.922
48.177
1.00
22.55
A


ATOM
2056
CZ3
TRP
A
262
109.134
83.332
46.359
1.00
22.37
A


ATOM
2057
CH2
TRP
A
262
108.636
84.106
47.427
1.00
27.32
A


ATOM
2058
C
TRP
A
262
113.345
81.504
46.891
1.00
21.59
A


ATOM
2059
O
TRP
A
262
113.003
81.383
48.077
1.00
21.98
A


ATOM
2060
N
LEU
A
263
112.806
80.767
45.927
1.00
24.29
A


ATOM
2061
CA
LEU
A
263
111.760
79.775
46.248
1.00
24.58
A


ATOM
2062
CB
LEU
A
263
111.270
79.050
44.985
1.00
23.15
A


ATOM
2063
CG
LEU
A
263
110.294
79.804
44.055
1.00
26.66
A


ATOM
2064
CD1
LEU
A
263
110.179
79.064
42.702
1.00
25.63
A


ATOM
2065
CD2
LEU
A
263
108.902
79.890
44.736
1.00
19.50
A


ATOM
2066
C
LEU
A
263
112.280
78.754
47.252
1.00
25.46
A


ATOM
2067
O
LEU
A
263
111.589
78.396
48.213
1.00
21.54
A


ATOM
2068
N
ASN
A
264
113.521
78.334
47.052
1.00
27.69
A


ATOM
2069
CA
ASN
A
264
114.138
77.339
47.923
1.00
26.89
A


ATOM
2070
CB
ASN
A
264
115.429
76.833
47.317
1.00
30.52
A


ATOM
2071
CG
ASN
A
264
115.869
75.523
47.906
1.00
37.07
A


ATOM
2072
OD1
ASN
A
264
117.047
75.355
48.181
1.00
41.85
A


ATOM
2073
ND2
ASN
A
264
114.936
74.576
48.093
1.00
34.54
A


ATOM
2074
C
ASN
A
264
114.426
77.843
49.308
1.00
31.72
A


ATOM
2075
O
ASN
A
264
114.734
77.040
50.186
1.00
29.93
A


ATOM
2076
N
GLU
A
265
114.368
79.161
49.518
1.00
23.33
A


ATOM
2077
CA
GLU
A
265
114.583
79.677
50.868
1.00
27.89
A


ATOM
2078
CB
GLU
A
265
115.256
81.057
50.817
1.00
36.54
A


ATOM
2079
CG
GLU
A
265
116.666
81.031
50.220
1.00
45.81
A


ATOM
2080
CD
GLU
A
265
117.203
82.426
49.890
1.00
52.24
A


ATOM
2081
OE1
GLU
A
265
116.412
83.284
49.448
1.00
55.22
A


ATOM
2082
OE2
GLU
A
265
118.421
82.659
50.053
1.00
59.70
A


ATOM
2083
C
GLU
A
265
113.240
79.794
51.617
1.00
31.76
A


ATOM
2084
O
GLU
A
265
113.210
80.100
52.800
1.00
31.51
A


ATOM
2085
N
GLN
A
266
112.131
79.578
50.917
1.00
25.12
A


ATOM
2086
CA
GLN
A
266
110.823
79.682
51.534
1.00
25.11
A


ATOM
2087
CB
GLN
A
266
109.769
80.120
50.492
1.00
22.70
A


ATOM
2088
CG
GLN
A
266
110.160
81.344
49.619
1.00
27.84
A


ATOM
2089
CD
GLN
A
266
110.749
82.499
50.434
1.00
30.91
A


ATOM
2090
OE1
GLN
A
266
110.138
82.961
51.386
1.00
29.87
A


ATOM
2091
NE2
GLN
A
266
111.949
82.952
50.065
1.00
23.36
A


ATOM
2092
C
GLN
A
266
110.402
78.343
52.166
1.00
29.30
A


ATOM
2093
O
GLN
A
266
110.826
77.247
51.730
1.00
24.87
A


ATOM
2094
N
ARG
A
267
109.585
78.444
53.212
1.00
15.92
A


ATOM
2095
CA
ARG
A
267
109.051
77.274
53.898
1.00
25.35
A


ATOM
2096
CB
ARG
A
267
108.588
77.693
55.292
1.00
33.71
A


ATOM
2097
CG
ARG
A
267
107.994
76.574
56.134
1.00
51.92
A


ATOM
2098
CD
ARG
A
267
107.907
76.988
57.616
1.00
62.06
A


ATOM
2099
NE
ARG
A
267
106.792
76.336
58.312
1.00
68.36
A


ATOM
2100
CZ
ARG
A
267
105.511
76.589
58.055
1.00
69.62
A


ATOM
2101
NH1
ARG
A
267
105.199
77.484
57.118
1.00
71.28
A


ATOM
2102
NH2
ARG
A
267
104.546
75.951
58.721
1.00
63.27
A


ATOM
2103
C
ARG
A
267
107.868
76.833
53.031
1.00
24.21
A


ATOM
2104
O
ARG
A
267
106.740
76.727
53.500
1.00
21.31
A


ATOM
2105
N
TYR
A
268
108.146
76.503
51.772
1.00
20.68
A


ATOM
2106
CA
TYR
A
268
107.077
76.220
50.844
1.00
19.61
A


ATOM
2107
CB
TYR
A
268
107.623
76.325
49.416
1.00
19.65
A


ATOM
2108
CG
TYR
A
268
108.603
75.213
49.054
1.00
20.48
A


ATOM
2109
CD1
TYR
A
268
108.141
73.919
48.758
1.00
14.04
A


ATOM
2110
CE1
TYR
A
268
109.038
72.914
48.336
1.00
17.07
A


ATOM
2111
CD2
TYR
A
268
109.988
75.469
48.950
1.00
18.73
A


ATOM
2112
CE2
TYR
A
268
110.896
74.462
48.562
1.00
10.52
A


ATOM
2113
CZ
TYR
A
268
110.400
73.191
48.251
1.00
18.28
A


ATOM
2114
OH
TYR
A
268
111.270
72.207
47.849
1.00
20.44
A


ATOM
2115
C
TYR
A
268
106.320
74.921
51.058
1.00
25.67
A


ATOM
2116
O
TYR
A
268
105.280
74.726
50.457
1.00
20.64
A


ATOM
2117
N
TYR
A
269
106.837
74.047
51.923
1.00
22.96
A


ATOM
2118
CA
TYR
A
269
106.192
72.764
52.224
1.00
22.56
A


ATOM
2119
CB
TYR
A
269
107.284
71.749
52.550
1.00
19.40
A


ATOM
2120
CG
TYR
A
269
108.279
72.324
53.538
1.00
25.20
A


ATOM
2121
CD1
TYR
A
269
108.002
72.326
54.913
1.00
21.63
A


ATOM
2122
CE1
TYR
A
269
108.856
72.951
55.823
1.00
19.42
A


ATOM
2123
CD2
TYR
A
269
109.452
72.970
53.098
1.00
23.97
A


ATOM
2124
CE2
TYR
A
269
110.310
73.620
54.010
1.00
18.32
A


ATOM
2125
CZ
TYR
A
269
110.002
73.600
55.359
1.00
24.42
A


ATOM
2126
OH
TYR
A
269
110.809
74.232
56.269
1.00
30.40
A


ATOM
2127
C
TYR
A
269
105.286
72.941
53.459
1.00
19.68
A


ATOM
2128
O
TYR
A
269
104.703
71.986
53.943
1.00
21.53
A


ATOM
2129
N
GLY
A
270
105.176
74.170
53.948
1.00
21.67
A


ATOM
2130
CA
GLY
A
270
104.406
74.456
55.146
1.00
17.59
A


ATOM
2131
C
GLY
A
270
102.977
73.953
55.184
1.00
25.69
A


ATOM
2132
O
GLY
A
270
102.532
73.482
56.232
1.00
24.34
A


ATOM
2133
N
GLY
A
271
102.266
73.984
54.057
1.00
19.73
A


ATOM
2134
CA
GLY
A
271
100.868
73.577
54.104
1.00
21.41
A


ATOM
2135
C
GLY
A
271
100.160
74.574
55.033
1.00
26.85
A


ATOM
2136
O
GLY
A
271
100.583
75.740
55.198
1.00
40.08
A


ATOM
2137
N
GLY
A
272
99.091
74.158
55.662
1.00
23.80
A


ATOM
2138
CA
GLY
A
272
98.437
75.085
56.568
1.00
26.88
A


ATOM
2139
C
GLY
A
272
97.305
75.856
55.915
1.00
23.11
A


ATOM
2140
O
GLY
A
272
97.123
75.787
54.712
1.00
21.50
A


ATOM
2141
N
TYR
A
273
96.567
76.618
56.710
1.00
17.15
A


ATOM
2142
CA
TYR
A
273
95.425
77.353
56.196
1.00
19.83
A


ATOM
2143
CB
TYR
A
273
94.836
78.198
57.313
1.00
21.62
A


ATOM
2144
CG
TYR
A
273
93.616
78.988
56.897
1.00
23.29
A


ATOM
2145
CD1
TYR
A
273
92.321
78.473
57.084
1.00
20.57
A


ATOM
2146
CE1
TYR
A
273
91.184
79.246
56.758
1.00
23.40
A


ATOM
2147
CD2
TYR
A
273
93.759
80.281
56.359
1.00
23.31
A


ATOM
2148
CE2
TYR
A
273
92.646
81.060
56.027
1.00
22.47
A


ATOM
2149
CZ
TYR
A
273
91.371
80.532
56.235
1.00
20.88
A


ATOM
2150
OH
TYR
A
273
90.314
81.304
55.896
1.00
20.65
A


ATOM
2151
C
TYR
A
273
95.747
78.224
54.976
1.00
23.07
A


ATOM
2152
O
TYR
A
273
96.764
78.920
54.931
1.00
14.66
A


ATOM
2153
N
GLY
A
274
94.891
78.150
53.966
1.00
23.18
A


ATOM
2154
CA
GLY
A
274
95.092
78.955
52.768
1.00
16.12
A


ATOM
2155
C
GLY
A
274
96.280
78.623
51.891
1.00
25.88
A


ATOM
2156
O
GLY
A
274
96.629
79.431
51.012
1.00
20.78
A


ATOM
2157
N
SER
A
275
96.907
77.458
52.080
1.00
14.86
A


ATOM
2158
CA
SER
A
275
98.089
77.131
51.270
1.00
20.55
A


ATOM
2159
CB
SER
A
275
99.074
76.297
52.077
1.00
16.52
A


ATOM
2160
OG
SER
A
275
98.428
75.061
52.389
1.00
21.60
A


ATOM
2161
C
SER
A
275
97.806
76.335
50.005
1.00
20.94
A


ATOM
2162
O
SER
A
275
98.746
75.961
49.318
1.00
17.86
A


ATOM
2163
N
THR
A
276
96.544
76.072
49.683
1.00
18.41
A


ATOM
2164
CA
THR
A
276
96.269
75.232
48.516
1.00
19.71
A


ATOM
2165
CB
THR
A
276
94.730
75.059
48.238
1.00
22.45
A


ATOM
2166
OG1
THR
A
276
94.192
76.301
47.838
1.00
51.89
A


ATOM
2167
CG2
THR
A
276
93.993
74.729
49.503
1.00
5.81
A


ATOM
2168
C
THR
A
276
96.967
75.607
47.228
1.00
21.51
A


ATOM
2169
O
THR
A
276
97.666
74.764
46.650
1.00
19.94
A


ATOM
2170
N
GLN
A
277
96.823
76.850
46.754
1.00
13.97
A


ATOM
2171
CA
GLN
A
277
97.463
77.189
45.485
1.00
15.27
A


ATOM
2172
CB
GLN
A
277
96.942
78.540
44.964
1.00
15.99
A


ATOM
2173
CG
GLN
A
277
95.421
78.529
44.563
1.00
8.40
A


ATOM
2174
CD
GLN
A
277
95.100
77.321
43.742
1.00
20.22
A


ATOM
2175
OE1
GLN
A
277
95.685
77.090
42.673
1.00
16.53
A


ATOM
2176
NE2
GLN
A
277
94.190
76.505
44.258
1.00
18.51
A


ATOM
2177
C
GLN
A
277
99.007
77.220
45.567
1.00
13.58
A


ATOM
2178
O
GLN
A
277
99.689
76.791
44.620
1.00
15.16
A


ATOM
2179
N
ALA
A
278
99.534
77.754
46.667
1.00
17.70
A


ATOM
2180
CA
ALA
A
278
100.996
77.842
46.833
1.00
17.58
A


ATOM
2181
CB
ALA
A
278
101.367
78.522
48.200
1.00
12.12
A


ATOM
2182
C
ALA
A
278
101.539
76.426
46.798
1.00
20.92
A


ATOM
2183
O
ALA
A
278
102.581
76.154
46.176
1.00
19.56
A


ATOM
2184
N
THR
A
279
100.821
75.524
47.477
1.00
18.75
A


ATOM
2185
CA
THR
A
279
101.254
74.136
47.553
1.00
15.04
A


ATOM
2186
CB
THR
A
279
100.380
73.341
48.554
1.00
23.60
A


ATOM
2187
OG1
THR
A
279
100.574
73.865
49.873
1.00
16.00
A


ATOM
2188
CG2
THR
A
279
100.755
71.832
48.531
1.00
18.05
A


ATOM
2189
C
THR
A
279
101.224
73.469
46.195
1.00
20.78
A


ATOM
2190
O
THR
A
279
102.224
72.894
45.758
1.00
14.58
A


ATOM
2191
N
PHE
A
280
100.080
73.544
45.514
1.00
14.85
A


ATOM
2192
CA
PHE
A
280
99.989
72.925
44.212
1.00
15.92
A


ATOM
2193
CB
PHE
A
280
98.601
73.198
43.604
1.00
15.13
A


ATOM
2194
CG
PHE
A
280
98.354
72.463
42.313
1.00
19.65
A


ATOM
2195
CD1
PHE
A
280
97.543
71.341
42.284
1.00
19.12
A


ATOM
2196
CD2
PHE
A
280
98.873
72.932
41.120
1.00
17.17
A


ATOM
2197
CE1
PHE
A
280
97.261
70.703
41.074
1.00
21.16
A


ATOM
2198
CE2
PHE
A
280
98.593
72.308
39.910
1.00
22.94
A


ATOM
2199
CZ
PHE
A
280
97.784
71.202
39.884
1.00
22.80
A


ATOM
2200
C
PHE
A
280
101.031
73.493
43.273
1.00
22.45
A


ATOM
2201
O
PHE
A
280
101.706
72.768
42.534
1.00
17.39
A


ATOM
2202
N
MET
A
281
101.157
74.808
43.284
1.00
15.49
A


ATOM
2203
CA
MET
A
281
102.061
75.445
42.339
1.00
19.30
A


ATOM
2204
CB
MET
A
281
101.681
76.939
42.169
1.00
16.61
A


ATOM
2205
CG
MET
A
281
100.322
77.212
41.527
1.00
18.78
A


ATOM
2206
SD
MET
A
281
100.020
76.356
39.946
1.00
24.71
A


ATOM
2207
CE
MET
A
281
101.567
76.615
39.056
1.00
22.61
A


ATOM
2208
C
MET
A
281
103.576
75.321
42.559
1.00
12.99
A


ATOM
2209
O
MET
A
281
104.316
75.044
41.593
1.00
20.36
A


ATOM
2210
N
VAL
A
282
104.036
75.481
43.800
1.00
20.60
A


ATOM
2211
CA
VAL
A
282
105.475
75.395
44.084
1.00
17.17
A


ATOM
2212
CB
VAL
A
282
105.795
75.806
45.562
1.00
16.00
A


ATOM
2213
CG1
VAL
A
282
105.366
74.677
46.522
1.00
16.44
A


ATOM
2214
CG2
VAL
A
282
107.331
76.108
45.740
1.00
20.08
A


ATOM
2215
C
VAL
A
282
105.974
73.961
43.790
1.00
16.69
A


ATOM
2216
O
VAL
A
282
107.058
73.777
43.230
1.00
16.91
A


ATOM
2217
N
PHE
A
283
105.184
72.943
44.134
1.00
13.38
A


ATOM
2218
CA
PHE
A
283
105.623
71.562
43.832
1.00
20.74
A


ATOM
2219
CB
PHE
A
283
104.821
70.541
44.684
1.00
13.63
A


ATOM
2220
CG
PHE
A
283
105.280
70.495
46.117
1.00
21.60
A


ATOM
2221
CD1
PHE
A
283
104.633
71.260
47.107
1.00
18.08
A


ATOM
2222
CD2
PHE
A
283
106.402
69.732
46.477
1.00
17.18
A


ATOM
2223
CE1
PHE
A
283
105.100
71.264
48.441
1.00
21.58
A


ATOM
2224
CE2
PHE
A
283
106.873
69.736
47.809
1.00
19.10
A


ATOM
2225
CZ
PHE
A
283
106.220
70.502
48.790
1.00
15.19
A


ATOM
2226
C
PHE
A
283
105.559
71.274
42.329
1.00
17.88
A


ATOM
2227
O
PHE
A
283
106.418
70.582
41.780
1.00
17.08
A


ATOM
2228
N
GLN
A
284
104.572
71.859
41.642
1.00
17.15
A


ATOM
2229
CA
GLN
A
284
104.505
71.696
40.195
1.00
15.23
A


ATOM
2230
CB
GLN
A
284
103.258
72.375
39.570
1.00
19.08
A


ATOM
2231
CG
GLN
A
284
103.135
72.123
38.053
1.00
18.61
A


ATOM
2232
CD
GLN
A
284
101.834
72.642
37.446
1.00
27.56
A


ATOM
2233
OE1
GLN
A
284
101.215
73.561
37.957
1.00
19.72
A


ATOM
2234
NE2
GLN
A
284
101.419
72.040
36.358
1.00
28.76
A


ATOM
2235
C
GLN
A
284
105.733
72.350
39.583
1.00
23.26
A


ATOM
2236
O
GLN
A
284
106.403
71.749
38.721
1.00
19.38
A


ATOM
2237
N
ALA
A
285
106.032
73.586
40.020
1.00
20.27
A


ATOM
2238
CA
ALA
A
285
107.169
74.314
39.474
1.00
22.72
A


ATOM
2239
CB
ALA
A
285
107.131
75.841
39.900
1.00
13.58
A


ATOM
2240
C
ALA
A
285
108.525
73.704
39.796
1.00
19.56
A


ATOM
2241
O
ALA
A
285
109.342
73.571
38.891
1.00
18.63
A


ATOM
2242
N
LEU
A
286
108.775
73.348
41.060
1.00
17.53
A


ATOM
2243
CA
LEU
A
286
110.059
72.729
41.426
1.00
22.71
A


ATOM
2244
CB
LEU
A
286
110.238
72.682
42.957
1.00
20.14
A


ATOM
2245
CG
LEU
A
286
110.337
74.074
43.563
1.00
26.93
A


ATOM
2246
CD1
LEU
A
286
110.528
74.003
45.093
1.00
20.46
A


ATOM
2247
CD2
LEU
A
286
111.470
74.793
42.896
1.00
21.22
A


ATOM
2248
C
LEU
A
286
110.181
71.322
40.832
1.00
18.99
A


ATOM
2249
O
LEU
A
286
111.286
70.866
40.558
1.00
23.33
A


ATOM
2250
N
ALA
A
287
109.058
70.631
40.629
1.00
20.11
A


ATOM
2251
CA
ALA
A
287
109.135
69.321
39.980
1.00
18.27
A


ATOM
2252
CB
ALA
A
287
107.786
68.606
40.005
1.00
16.55
A


ATOM
2253
C
ALA
A
287
109.519
69.613
38.530
1.00
25.56
A


ATOM
2254
O
ALA
A
287
110.381
68.942
37.927
1.00
19.06
A


ATOM
2255
N
GLN
A
288
108.907
70.634
37.935
1.00
18.68
A


ATOM
2256
CA
GLN
A
288
109.249
70.877
36.536
1.00
16.52
A


ATOM
2257
CB
GLN
A
288
108.392
71.977
35.894
1.00
23.21
A


ATOM
2258
CG
GLN
A
288
108.681
72.106
34.390
1.00
20.99
A


ATOM
2259
CD
GLN
A
288
108.188
70.889
33.579
1.00
29.85
A


ATOM
2260
OE1
GLN
A
288
108.896
70.370
32.704
1.00
29.90
A


ATOM
2261
NE2
GLN
A
288
106.972
70.448
33.863
1.00
16.47
A


ATOM
2262
C
GLN
A
288
110.719
71.258
36.345
1.00
22.79
A


ATOM
2263
O
GLN
A
288
111.330
70.907
35.335
1.00
16.55
A


ATOM
2264
N
TYR
A
289
111.262
72.009
37.287
1.00
19.12
A


ATOM
2265
CA
TYR
A
289
112.654
72.405
37.203
1.00
18.73
A


ATOM
2266
CB
TYR
A
289
113.021
73.260
38.403
1.00
18.19
A


ATOM
2267
CG
TYR
A
289
114.482
73.677
38.476
1.00
26.27
A


ATOM
2268
CD1
TYR
A
289
114.898
74.932
38.001
1.00
26.70
A


ATOM
2269
CE1
TYR
A
289
116.232
75.332
38.072
1.00
25.07
A


ATOM
2270
CD2
TYR
A
289
115.447
72.820
39.024
1.00
23.61
A


ATOM
2271
CE2
TYR
A
289
116.796
73.199
39.098
1.00
26.68
A


ATOM
2272
CZ
TYR
A
289
117.178
74.469
38.615
1.00
31.54
A


ATOM
2273
OH
TYR
A
289
118.491
74.859
38.678
1.00
22.55
A


ATOM
2274
C
TYR
A
289
113.541
71.142
37.207
1.00
25.66
A


ATOM
2275
O
TYR
A
289
114.502
71.042
36.424
1.00
21.35
A


ATOM
2276
N
GLN
A
290
113.224
70.195
38.095
1.00
22.15
A


ATOM
2277
CA
GLN
A
290
114.013
68.974
38.200
1.00
25.92
A


ATOM
2278
CB
GLN
A
290
113.751
68.295
39.535
1.00
16.82
A


ATOM
2279
CG
GLN
A
290
114.234
69.185
40.698
1.00
20.11
A


ATOM
2280
CD
GLN
A
290
113.869
68.620
42.062
1.00
28.81
A


ATOM
2281
OE1
GLN
A
290
114.594
67.805
42.611
1.00
23.19
A


ATOM
2282
NE2
GLN
A
290
112.740
69.050
42.603
1.00
19.24
A


ATOM
2283
C
GLN
A
290
113.792
68.037
37.053
1.00
24.56
A


ATOM
2284
O
GLN
A
290
114.690
67.272
36.654
1.00
22.99
A


ATOM
2285
N
LYS
A
291
112.604
68.100
36.485
1.00
28.20
A


ATOM
2286
CA
LYS
A
291
112.340
67.233
35.349
1.00
27.92
A


ATOM
2287
CB
LYS
A
291
110.879
67.253
34.960
1.00
25.58
A


ATOM
2288
CG
LYS
A
291
110.640
66.179
33.951
1.00
35.43
A


ATOM
2289
CD
LYS
A
291
109.644
66.601
32.962
1.00
47.83
A


ATOM
2290
CE
LYS
A
291
109.438
65.497
31.950
1.00
53.06
A


ATOM
2291
NZ
LYS
A
291
108.781
64.338
32.602
1.00
54.26
A


ATOM
2292
C
LYS
A
291
113.128
67.716
34.138
1.00
30.13
A


ATOM
2293
O
LYS
A
291
113.543
66.915
33.313
1.00
24.65
A


ATOM
2294
N
ASP
A
292
113.305
69.031
34.028
1.00
21.05
A


ATOM
2295
CA
ASP
A
292
114.016
69.633
32.891
1.00
25.19
A


ATOM
2296
CB
ASP
A
292
113.610
71.109
32.757
1.00
23.19
A


ATOM
2297
CG
ASP
A
292
112.222
71.299
32.158
1.00
33.23
A


ATOM
2298
OD1
ASP
A
292
111.626
70.308
31.703
1.00
27.34
A


ATOM
2299
OD2
ASP
A
292
111.737
72.452
32.131
1.00
27.83
A


ATOM
2300
C
ASP
A
292
115.556
69.584
33.008
1.00
23.90
A


ATOM
2301
O
ASP
A
292
116.275
69.573
32.017
1.00
22.32
A


ATOM
2302
N
ALA
A
293
116.034
69.604
34.236
1.00
20.48
A


ATOM
2303
CA
ALA
A
293
117.451
69.602
34.558
1.00
28.06
A


ATOM
2304
CB
ALA
A
293
117.602
69.472
36.065
1.00
20.35
A


ATOM
2305
C
ALA
A
293
118.355
68.564
33.838
1.00
29.15
A


ATOM
2306
O
ALA
A
293
119.480
68.885
33.427
1.00
30.95
A


ATOM
2307
N
PRO
A
294
117.913
67.302
33.732
1.00
29.07
A


ATOM
2308
CD
PRO
A
294
116.811
66.630
34.437
1.00
30.66
A


ATOM
2309
CA
PRO
A
294
118.777
66.331
33.051
1.00
28.43
A


ATOM
2310
CB
PRO
A
294
118.013
65.019
33.196
1.00
36.12
A


ATOM
2311
CG
PRO
A
294
117.307
65.185
34.516
1.00
34.80
A


ATOM
2312
C
PRO
A
294
119.062
66.686
31.593
1.00
34.08
A


ATOM
2313
O
PRO
A
294
120.059
66.245
31.042
1.00
37.55
A


ATOM
2314
N
SER
A
295
118.206
67.496
30.970
1.00
31.59
A


ATOM
2315
CA
SER
A
295
118.412
67.895
29.570
1.00
32.05
A


ATOM
2316
CB
SER
A
295
117.053
68.031
28.827
1.00
37.77
A


ATOM
2317
OG
SER
A
295
116.413
66.791
28.542
1.00
38.35
A


ATOM
2318
C
SER
A
295
119.141
69.246
29.412
1.00
33.12
A


ATOM
2319
O
SER
A
295
119.543
69.592
28.310
1.00
27.93
A


ATOM
2320
N
ASP
A
296
119.310
70.014
30.490
1.00
26.80
A


ATOM
2321
CA
ASP
A
296
119.913
71.328
30.352
1.00
30.74
A


ATOM
2322
CB
ASP
A
296
118.782
72.415
30.256
1.00
27.90
A


ATOM
2323
CG
ASP
A
296
117.839
72.252
28.986
1.00
41.52
A


ATOM
2324
OD1
ASP
A
296
118.246
72.564
27.834
1.00
38.56
A


ATOM
2325
OD2
ASP
A
296
116.655
71.816
29.121
1.00
44.60
A


ATOM
2326
C
ASP
A
296
120.862
71.604
31.525
1.00
31.76
A


ATOM
2327
O
ASP
A
296
120.426
71.809
32.661
1.00
31.22
A


ATOM
2328
N
HIS
A
297
122.161
71.613
31.240
1.00
27.39
A


ATOM
2329
CA
HIS
A
297
123.221
71.862
32.231
1.00
31.02
A


ATOM
2330
CB
HIS
A
297
124.562
72.043
31.514
1.00
39.25
A


ATOM
2331
CG
HIS
A
297
125.466
70.858
31.577
1.00
47.23
A


ATOM
2332
CD2
HIS
A
297
126.818
70.762
31.501
1.00
46.79
A


ATOM
2333
ND1
HIS
A
297
125.005
69.597
31.863
1.00
48.20
A


ATOM
2334
CE1
HIS
A
297
126.036
68.777
31.990
1.00
54.99
A


ATOM
2335
NE2
HIS
A
297
127.146
69.462
31.778
1.00
46.54
A


ATOM
2336
C
HIS
A
297
122.999
73.125
33.043
1.00
27.48
A


ATOM
2337
O
HIS
A
297
123.412
73.224
34.203
1.00
28.00
A


ATOM
2338
N
GLN
A
298
122.396
74.117
32.401
1.00
22.60
A


ATOM
2339
CA
GLN
A
298
122.174
75.396
33.046
1.00
25.08
A


ATOM
2340
CB
GLN
A
298
121.569
76.409
32.065
1.00
30.41
A


ATOM
2341
CG
GLN
A
298
122.332
76.669
30.783
1.00
34.42
A


ATOM
2342
CD
GLN
A
298
121.872
75.791
29.603
1.00
37.26
A


ATOM
2343
OE1
GLN
A
298
121.932
76.217
28.444
1.00
36.98
A


ATOM
2344
NE2
GLN
A
298
121.429
74.558
29.897
1.00
32.64
A


ATOM
2345
C
GLN
A
298
121.276
75.302
34.280
1.00
26.28
A


ATOM
2346
O
GLN
A
298
121.322
76.182
35.129
1.00
23.86
A


ATOM
2347
N
GLU
A
299
120.448
74.263
34.374
1.00
22.71
A


ATOM
2348
CA
GLU
A
299
119.556
74.107
35.525
1.00
29.56
A


ATOM
2349
CB
GLU
A
299
118.247
73.497
35.081
1.00
27.09
A


ATOM
2350
CG
GLU
A
299
117.445
74.443
34.288
1.00
42.75
A


ATOM
2351
CD
GLU
A
299
116.372
73.723
33.574
1.00
52.61
A


ATOM
2352
OE1
GLU
A
299
115.342
73.415
34.208
1.00
64.24
A


ATOM
2353
OE2
GLU
A
299
116.565
73.443
32.377
1.00
59.69
A


ATOM
2354
C
GLU
A
299
120.251
73.185
36.491
1.00
30.34
A


ATOM
2355
O
GLU
A
299
119.841
72.042
36.706
1.00
25.19
A


ATOM
2356
N
LEU
A
300
121.289
73.724
37.099
1.00
26.72
A


ATOM
2357
CA
LEU
A
300
122.164
72.960
37.960
1.00
26.16
A


ATOM
2358
CB
LEU
A
300
123.621
73.384
37.693
1.00
24.26
A


ATOM
2359
CG
LEU
A
300
124.040
74.875
37.817
1.00
23.41
A


ATOM
2360
CD1
LEU
A
300
122.894
75.782
38.110
1.00
13.62
A


ATOM
2361
CD2
LEU
A
300
125.064
75.023
38.845
1.00
23.86
A


ATOM
2362
C
LEU
A
300
121.930
73.005
39.439
1.00
31.66
A


ATOM
2363
O
LEU
A
300
122.685
72.360
40.165
1.00
24.27
A


ATOM
2364
N
ASN
A
301
120.899
73.717
39.895
1.00
20.86
A


ATOM
2365
CA
ASN
A
301
120.674
73.844
41.335
1.00
27.51
A


ATOM
2366
CB
ASN
A
301
119.685
74.974
41.629
1.00
27.49
A


ATOM
2367
CG
ASN
A
301
120.338
76.367
41.606
1.00
41.50
A


ATOM
2368
OD1
ASN
A
301
121.524
76.531
41.948
1.00
42.07
A


ATOM
2369
ND2
ASN
A
301
119.546
77.386
41.248
1.00
40.89
A


ATOM
2370
C
ASN
A
301
120.139
72.561
41.946
1.00
27.36
A


ATOM
2371
O
ASN
A
301
119.478
71.775
41.255
1.00
20.70
A


ATOM
2372
N
LEU
A
302
120.455
72.338
43.227
1.00
28.60
A


ATOM
2373
CA
LEU
A
302
119.962
71.168
43.945
1.00
24.52
A


ATOM
2374
CB
LEU
A
302
121.038
70.593
44.894
1.00
29.03
A


ATOM
2375
CG
LEU
A
302
120.446
69.605
45.935
1.00
27.91
A


ATOM
2376
CD1
LEU
A
302
119.494
68.711
45.254
1.00
18.13
A


ATOM
2377
CD2
LEU
A
302
121.464
68.809
46.627
1.00
28.47
A


ATOM
2378
C
LEU
A
302
118.742
71.586
44.767
1.00
21.88
A


ATOM
2379
O
LEU
A
302
118.888
72.203
45.817
1.00
20.14
A


ATOM
2380
N
ASP
A
303
117.540
71.265
44.306
1.00
18.15
A


ATOM
2381
CA
ASP
A
303
116.335
71.611
45.095
1.00
29.52
A


ATOM
2382
CB
ASP
A
303
115.472
72.630
44.334
1.00
25.17
A


ATOM
2383
CG
ASP
A
303
115.065
72.121
42.996
1.00
37.89
A


ATOM
2384
OD1
ASP
A
303
115.528
71.037
42.637
1.00
54.39
A


ATOM
2385
OD2
ASP
A
303
114.298
72.780
42.279
1.00
57.27
A


ATOM
2386
C
ASP
A
303
115.580
70.289
45.373
1.00
22.29
A


ATOM
2387
O
ASP
A
303
114.347
70.216
45.577
1.00
22.75
A


ATOM
2388
N
VAL
A
304
116.382
69.240
45.425
1.00
21.19
A


ATOM
2389
CA
VAL
A
304
115.925
67.870
45.624
1.00
25.10
A


ATOM
2390
CB
VAL
A
304
116.935
66.930
44.914
1.00
21.32
A


ATOM
2391
CG1
VAL
A
304
118.001
66.444
45.851
1.00
13.87
A


ATOM
2392
CG2
VAL
A
304
116.235
65.842
44.282
1.00
42.05
A


ATOM
2393
C
VAL
A
304
115.770
67.499
47.102
1.00
19.73
A


ATOM
2394
O
VAL
A
304
116.219
68.242
47.961
1.00
19.32
A


ATOM
2395
N
SER
A
305
115.136
66.360
47.402
1.00
21.55
A


ATOM
2396
CA
SER
A
305
114.969
65.904
48.794
1.00
24.92
A


ATOM
2397
CB
SER
A
305
114.202
64.572
48.838
1.00
31.82
A


ATOM
2398
OG
SER
A
305
112.797
64.716
48.530
1.00
45.40
A


ATOM
2399
C
SER
A
305
116.366
65.696
49.401
1.00
32.11
A


ATOM
2400
O
SER
A
305
117.187
64.961
48.836
1.00
19.54
A


ATOM
2401
N
LEU
A
306
116.649
66.330
50.535
1.00
25.35
A


ATOM
2402
CA
LEU
A
306
117.962
66.173
51.161
1.00
30.33
A


ATOM
2403
CB
LEU
A
306
118.422
67.488
51.831
1.00
35.23
A


ATOM
2404
CG
LEU
A
306
118.886
68.694
50.987
1.00
33.58
A


ATOM
2405
CD1
LEU
A
306
119.870
68.352
49.838
1.00
29.73
A


ATOM
2406
CD2
LEU
A
306
117.665
69.257
50.434
1.00
39.98
A


ATOM
2407
C
LEU
A
306
117.943
65.050
52.208
1.00
37.07
A


ATOM
2408
O
LEU
A
306
116.874
64.515
52.547
1.00
34.41
A


ATOM
2409
N
GLN
A
307
119.107
64.670
52.722
1.00
37.78
A


ATOM
2410
CA
GLN
A
307
119.102
63.615
53.734
1.00
47.24
A


ATOM
2411
CB
GLN
A
307
120.507
63.110
54.035
1.00
52.95
A


ATOM
2412
CG
GLN
A
307
120.931
61.932
53.174
1.00
64.46
A


ATOM
2413
CD
GLN
A
307
121.481
62.371
51.846
1.00
70.87
A


ATOM
2414
OE1
GLN
A
307
122.341
63.253
51.785
1.00
76.28
A


ATOM
2415
NE2
GLN
A
307
121.001
61.755
50.767
1.00
77.08
A


ATOM
2416
C
GLN
A
307
118.482
64.115
55.021
1.00
42.93
A


ATOM
2417
O
GLN
A
307
118.665
65.308
55.340
1.00
42.42
A


ATOM
2418
OXT
GLN
A
307
117.838
63.294
55.699
1.00
44.41
A


ATOM
2419
CB
ALA
B
1
64.181
107.666
4.123
1.00
39.20
B


ATOM
2420
C
ALA
B
1
62.836
105.816
3.113
1.00
41.03
B


ATOM
2421
O
ALA
B
1
61.659
105.713
3.477
1.00
42.08
B


ATOM
2422
N
ALA
B
1
62.413
108.231
2.458
1.00
40.18
B


ATOM
2423
CA
ALA
B
1
63.447
107.201
2.861
1.00
43.96
B


ATOM
2424
N
ILE
B
2
63.636
104.759
2.923
1.00
39.63
B


ATOM
2425
CA
ILE
B
2
63.175
103.383
3.141
1.00
37.01
B


ATOM
2426
CB
ILE
B
2
64.245
102.349
2.700
1.00
32.57
B


ATOM
2427
CG2
ILE
B
2
63.775
100.936
3.064
1.00
35.80
B


ATOM
2428
CG1
ILE
B
2
64.457
102.422
1.178
1.00
34.01
B


ATOM
2429
CD1
ILE
B
2
65.479
101.372
0.650
1.00
31.21
B


ATOM
2430
C
ILE
B
2
62.831
103.151
4.633
1.00
38.27
B


ATOM
2431
O
ILE
B
2
63.565
103.577
5.528
1.00
32.71
B


ATOM
2432
N
SER
B
3
61.714
102.486
4.897
1.00
30.65
B


ATOM
2433
CA
SER
B
3
61.316
102.226
6.272
1.00
26.19
B


ATOM
2434
CB
SER
B
3
60.245
103.236
6.689
1.00
31.79
B


ATOM
2435
OG
SER
B
3
59.060
103.020
5.926
1.00
27.35
B


ATOM
2436
C
SER
B
3
60.744
100.826
6.435
1.00
30.38
B


ATOM
2437
O
SER
B
3
60.495
100.132
5.446
1.00
28.89
B


ATOM
2438
N
CYS
B
4
60.559
100.410
7.690
1.00
27.89
B


ATOM
2439
CA
CYS
B
4
59.941
99.127
8.003
1.00
27.29
B


ATOM
2440
C
CYS
B
4
58.609
99.528
8.600
1.00
26.13
B


ATOM
2441
O
CYS
B
4
58.446
100.666
9.061
1.00
29.64
B


ATOM
2442
CB
CYS
B
4
60.731
98.328
9.056
1.00
27.46
B


ATOM
2443
SG
CYS
B
4
62.214
97.531
8.369
1.00
27.76
B


ATOM
2444
N
GLY
B
5
57.648
98.622
8.561
1.00
22.65
B


ATOM
2445
CA
GLY
B
5
56.366
98.916
9.155
1.00
23.57
B


ATOM
2446
C
GLY
B
5
56.452
98.570
10.643
1.00
24.00
B


ATOM
2447
O
GLY
B
5
57.504
98.199
11.181
1.00
23.18
B


ATOM
2448
N
SER
B
6
55.322
98.698
11.313
1.00
21.65
B


ATOM
2449
CA
SER
B
6
55.204
98.441
12.733
1.00
22.23
B


ATOM
2450
CB
SER
B
6
53.722
98.511
13.098
1.00
27.49
B


ATOM
2451
OG
SER
B
6
53.543
98.605
14.483
1.00
33.82
B


ATOM
2452
C
SER
B
6
55.785
97.062
13.091
1.00
26.45
B


ATOM
2453
O
SER
B
6
55.489
96.081
12.439
1.00
22.14
B


ATOM
2454
N
PRO
B
7
56.639
96.980
14.122
1.00
26.74
B


ATOM
2455
CD
PRO
B
7
57.230
98.026
14.973
1.00
25.89
B


ATOM
2456
CA
PRO
B
7
57.191
95.661
14.468
1.00
25.01
B


ATOM
2457
CB
PRO
B
7
58.175
95.984
15.592
1.00
22.52
B


ATOM
2458
CG
PRO
B
7
57.589
97.228
16.232
1.00
26.49
B


ATOM
2459
C
PRO
B
7
56.133
94.627
14.894
1.00
27.04
B


ATOM
2460
O
PRO
B
7
55.061
94.961
15.414
1.00
24.19
B


ATOM
2461
N
PRO
B
8
56.439
93.345
14.700
1.00
24.37
B


ATOM
2462
CD
PRO
B
8
57.540
92.754
13.936
1.00
26.75
B


ATOM
2463
CA
PRO
B
8
55.431
92.366
15.099
1.00
25.28
B


ATOM
2464
CB
PRO
B
8
55.974
91.048
14.538
1.00
31.15
B


ATOM
2465
CG
PRO
B
8
57.401
91.314
14.245
1.00
33.33
B


ATOM
2466
C
PRO
B
8
55.228
92.382
16.613
1.00
30.70
B


ATOM
2467
O
PRO
B
8
56.180
92.527
17.364
1.00
31.22
B


ATOM
2468
N
PRO
B
9
53.971
92.314
17.074
1.00
30.39
B


ATOM
2469
CD
PRO
B
9
52.724
92.280
16.275
1.00
37.82
B


ATOM
2470
CA
PRO
B
9
53.686
92.322
18.518
1.00
32.22
B


ATOM
2471
CB
PRO
B
9
52.155
92.415
18.589
1.00
31.66
B


ATOM
2472
CG
PRO
B
9
51.705
91.731
17.265
1.00
31.37
B


ATOM
2473
C
PRO
B
9
54.190
91.062
19.209
1.00
26.54
B


ATOM
2474
O
PRO
B
9
54.542
90.072
18.563
1.00
23.13
B


ATOM
2475
N
ILE
B
10
54.214
91.111
20.526
1.00
25.96
B


ATOM
2476
CA
ILE
B
10
54.651
89.969
21.302
1.00
34.36
B


ATOM
2477
CB
ILE
B
10
56.150
90.068
21.770
1.00
24.92
B


ATOM
2478
CG2
ILE
B
10
56.398
91.363
22.562
1.00
24.04
B


ATOM
2479
CG1
ILE
B
10
56.491
88.795
22.581
1.00
30.43
B


ATOM
2480
CD1
ILE
B
10
57.991
88.536
22.813
1.00
24.83
B


ATOM
2481
C
ILE
B
10
53.753
89.810
22.511
1.00
33.31
B


ATOM
2482
O
ILE
B
10
53.743
90.647
23.412
1.00
32.83
B


ATOM
2483
N
LEU
B
11
52.992
88.724
22.525
1.00
30.71
B


ATOM
2484
CA
LEU
B
11
52.108
88.483
23.659
1.00
34.63
B


ATOM
2485
CB
LEU
B
11
51.217
87.277
23.365
1.00
36.50
B


ATOM
2486
CG
LEU
B
11
50.279
87.435
22.161
1.00
41.37
B


ATOM
2487
CD1
LEU
B
11
49.708
86.073
21.769
1.00
42.99
B


ATOM
2488
CD2
LEU
B
11
49.142
88.425
22.495
1.00
39.65
B


ATOM
2489
C
LEU
B
11
52.912
88.247
24.946
1.00
33.29
B


ATOM
2490
O
LEU
B
11
53.905
87.517
24.948
1.00
28.06
B


ATOM
2491
N
ASN
B
12
52.463
88.872
26.032
1.00
31.32
B


ATOM
2492
CA
ASN
B
12
53.086
88.753
27.328
1.00
30.29
B


ATOM
2493
CB
ASN
B
12
53.025
87.303
27.802
1.00
32.27
B


ATOM
2494
CG
ASN
B
12
51.612
86.875
28.083
1.00
38.86
B


ATOM
2495
OD1
ASN
B
12
50.917
87.526
28.864
1.00
38.55
B


ATOM
2496
ND2
ASN
B
12
51.161
85.805
27.431
1.00
34.66
B


ATOM
2497
C
ASN
B
12
54.510
89.250
27.371
1.00
32.36
B


ATOM
2498
O
ASN
B
12
55.277
88.858
28.239
1.00
32.55
B


ATOM
2499
N
GLY
B
13
54.849
90.130
26.439
1.00
28.46
B


ATOM
2500
CA
GLY
B
13
56.187
90.671
26.400
1.00
28.72
B


ATOM
2501
C
GLY
B
13
56.147
92.166
26.169
1.00
29.82
B


ATOM
2502
O
GLY
B
13
55.075
92.780
26.153
1.00
34.03
B


ATOM
2503
N
ARG
B
14
57.324
92.742
25.983
1.00
28.15
B


ATOM
2504
CA
ARG
B
14
57.486
94.166
25.739
1.00
31.15
B


ATOM
2505
CB
ARG
B
14
58.198
94.882
26.897
1.00
34.62
B


ATOM
2506
CG
ARG
B
14
57.388
95.196
28.116
1.00
41.44
B


ATOM
2507
CD
ARG
B
14
58.240
95.962
29.112
1.00
42.08
B


ATOM
2508
NE
ARG
B
14
57.526
96.080
30.373
1.00
49.55
B


ATOM
2509
CZ
ARG
B
14
58.023
96.595
31.487
1.00
48.40
B


ATOM
2510
NH1
ARG
B
14
59.261
97.065
31.509
1.00
40.73
B


ATOM
2511
NH2
ARG
B
14
57.276
96.602
32.593
1.00
56.30
B


ATOM
2512
C
ARG
B
14
58.409
94.342
24.567
1.00
23.56
B


ATOM
2513
O
ARG
B
14
59.232
93.480
24.280
1.00
23.51
B


ATOM
2514
N
ILE
B
15
58.319
95.516
23.955
1.00
27.48
B


ATOM
2515
CA
ILE
B
15
59.171
95.893
22.841
1.00
26.75
B


ATOM
2516
CB
ILE
B
15
58.345
96.176
21.580
1.00
26.32
B


ATOM
2517
CG2
ILE
B
15
59.250
96.815
20.497
1.00
29.30
B


ATOM
2518
CG1
ILE
B
15
57.656
94.902
21.122
1.00
30.62
B


ATOM
2519
CD1
ILE
B
15
56.760
95.100
19.889
1.00
35.23
B


ATOM
2520
C
ILE
B
15
59.767
97.212
23.279
1.00
26.59
B


ATOM
2521
O
ILE
B
15
59.050
98.050
23.818
1.00
30.94
B


ATOM
2522
N
SER
B
16
61.055
97.409
23.036
1.00
23.15
B


ATOM
2523
CA
SER
B
16
61.709
98.654
23.393
1.00
32.61
B


ATOM
2524
CB
SER
B
16
63.187
98.567
23.057
1.00
29.32
B


ATOM
2525
OG
SER
B
16
63.359
98.151
21.717
1.00
35.31
B


ATOM
2526
C
SER
B
16
61.082
99.839
22.639
1.00
41.27
B


ATOM
2527
O
SER
B
16
60.466
99.674
21.576
1.00
36.78
B


ATOM
2528
N
TYR
B
17
61.260
101.035
23.193
1.00
42.89
B


ATOM
2529
CA
TYR
B
17
60.719
102.267
22.624
1.00
46.29
B


ATOM
2530
CB
TYR
B
17
60.932
103.436
23.623
1.00
44.99
B


ATOM
2531
CG
TYR
B
17
60.026
103.380
24.865
1.00
47.02
B


ATOM
2532
CD1
TYR
B
17
60.510
103.694
26.151
1.00
49.03
B


ATOM
2533
CE1
TYR
B
17
59.664
103.655
27.283
1.00
48.52
B


ATOM
2534
CD2
TYR
B
17
58.686
103.025
24.748
1.00
51.67
B


ATOM
2535
CE2
TYR
B
17
57.833
102.974
25.861
1.00
53.32
B


ATOM
2536
CZ
TYR
B
17
58.319
103.292
27.122
1.00
56.82
B


ATOM
2537
OH
TYR
B
17
57.430
103.259
28.190
1.00
55.53
B


ATOM
2538
C
TYR
B
17
61.397
102.551
21.285
1.00
46.15
B


ATOM
2539
O
TYR
B
17
62.600
102.375
21.146
1.00
50.00
B


ATOM
2540
N
TYR
B
18
60.623
102.952
20.281
1.00
46.05
B


ATOM
2541
CA
TYR
B
18
61.204
103.270
18.975
1.00
44.93
B


ATOM
2542
CB
TYR
B
18
61.076
102.076
18.010
1.00
37.78
B


ATOM
2543
CG
TYR
B
18
59.645
101.669
17.711
1.00
37.10
B


ATOM
2544
CD1
TYR
B
18
58.890
102.343
16.750
1.00
31.24
B


ATOM
2545
CE1
TYR
B
18
57.573
101.980
16.501
1.00
34.43
B


ATOM
2546
CD2
TYR
B
18
59.040
100.622
18.415
1.00
34.57
B


ATOM
2547
CE2
TYR
B
18
57.730
100.255
18.184
1.00
36.06
B


ATOM
2548
CZ
TYR
B
18
57.000
100.939
17.218
1.00
35.02
B


ATOM
2549
OH
TYR
B
18
55.706
100.565
16.967
1.00
33.22
B


ATOM
2550
C
TYR
B
18
60.524
104.514
18.375
1.00
40.62
B


ATOM
2551
O
TYR
B
18
59.356
104.784
18.632
1.00
40.57
B


ATOM
2552
N
SER
B
19
61.280
105.260
17.586
1.00
42.39
B


ATOM
2553
CA
SER
B
19
60.786
106.464
16.931
1.00
49.17
B


ATOM
2554
CB
SER
B
19
61.922
107.472
16.789
1.00
49.59
B


ATOM
2555
OG
SER
B
19
63.070
106.843
16.237
1.00
54.17
B


ATOM
2556
C
SER
B
19
60.213
106.155
15.540
1.00
51.20
B


ATOM
2557
O
SER
B
19
60.569
105.165
14.900
1.00
42.62
B


ATOM
2558
N
THR
B
20
59.347
107.041
15.073
1.00
48.91
B


ATOM
2559
CA
THR
B
20
58.706
106.898
13.783
1.00
49.40
B


ATOM
2560
CB
THR
B
20
57.166
107.002
13.991
1.00
52.73
B


ATOM
2561
OG1
THR
B
20
56.483
106.279
12.968
1.00
60.25
B


ATOM
2562
CG2
THR
B
20
56.714
108.456
13.943
1.00
51.71
B


ATOM
2563
C
THR
B
20
59.222
108.022
12.844
1.00
47.48
B


ATOM
2564
O
THR
B
20
59.460
109.147
13.280
1.00
50.56
B


ATOM
2565
N
PRO
B
21
59.427
107.720
11.551
1.00
46.52
B


ATOM
2566
CD
PRO
B
21
59.801
108.745
10.551
1.00
44.33
B


ATOM
2567
CA
PRO
B
21
59.204
106.417
10.909
1.00
43.20
B


ATOM
2568
CB
PRO
B
21
59.137
106.769
9.422
1.00
43.49
B


ATOM
2569
CG
PRO
B
21
60.103
107.918
9.305
1.00
44.19
B


ATOM
2570
C
PRO
B
21
60.342
105.456
11.248
1.00
37.71
B


ATOM
2571
O
PRO
B
21
61.393
105.891
11.674
1.00
38.42
B


ATOM
2572
N
THR
B
22
60.123
104.155
11.067
1.00
40.00
B


ATOM
2573
CA
THR
B
22
61.139
103.150
11.382
1.00
37.29
B


ATOM
2574
CB
THR
B
22
60.521
101.715
11.578
1.00
33.63
B


ATOM
2575
OG1
THR
B
22
59.497
101.719
12.600
1.00
36.55
B


ATOM
2576
CG2
THR
B
22
61.626
100.734
11.963
1.00
26.59
B


ATOM
2577
C
THR
B
22
62.143
103.079
10.221
1.00
35.94
B


ATOM
2578
O
THR
B
22
61.851
102.484
9.183
1.00
33.29
B


ATOM
2579
N
ALA
B
23
63.326
103.663
10.410
1.00
33.77
B


ATOM
2580
CA
ALA
B
23
64.367
103.677
9.380
1.00
30.19
B


ATOM
2581
CB
ALA
B
23
65.314
104.852
9.615
1.00
28.90
B


ATOM
2582
C
ALA
B
23
65.192
102.399
9.300
1.00
30.98
B


ATOM
2583
O
ALA
B
23
65.331
101.693
10.284
1.00
23.97
B


ATOM
2584
N
VAL
B
24
65.736
102.127
8.112
1.00
27.61
B


ATOM
2585
CA
VAL
B
24
66.591
100.982
7.874
1.00
26.92
B


ATOM
2586
CB
VAL
B
24
67.197
101.042
6.460
1.00
29.41
B


ATOM
2587
CG1
VAL
B
24
68.357
100.043
6.326
1.00
28.58
B


ATOM
2588
CG2
VAL
B
24
66.107
100.729
5.430
1.00
26.92
B


ATOM
2589
C
VAL
B
24
67.707
101.091
8.898
1.00
32.79
B


ATOM
2590
O
VAL
B
24
68.266
102.166
9.077
1.00
26.10
B


ATOM
2591
N
GLY
B
25
68.026
100.000
9.586
1.00
28.01
B


ATOM
2592
CA
GLY
B
25
69.070
100.081
10.596
1.00
31.93
B


ATOM
2593
C
GLY
B
25
68.516
100.210
12.007
1.00
34.29
B


ATOM
2594
O
GLY
B
25
69.251
100.080
12.966
1.00
28.44
B


ATOM
2595
N
THR
B
26
67.227
100.486
12.148
1.00
26.99
B


ATOM
2596
CA
THR
B
26
66.650
100.568
13.474
1.00
29.37
B


ATOM
2597
CB
THR
B
26
65.179
100.994
13.403
1.00
30.78
B


ATOM
2598
OG1
THR
B
26
65.111
102.295
12.808
1.00
26.87
B


ATOM
2599
CG2
THR
B
26
64.524
101.000
14.833
1.00
23.56
B


ATOM
2600
C
THR
B
26
66.713
99.183
14.136
1.00
32.07
B


ATOM
2601
O
THR
B
26
66.462
98.171
13.473
1.00
28.44
B


ATOM
2602
N
VAL
B
27
67.056
99.143
15.429
1.00
28.90
B


ATOM
2603
CA
VAL
B
27
67.124
97.891
16.200
1.00
29.71
B


ATOM
2604
CB
VAL
B
27
68.507
97.703
16.858
1.00
36.67
B


ATOM
2605
CG1
VAL
B
27
68.477
96.507
17.808
1.00
31.87
B


ATOM
2606
CG2
VAL
B
27
69.554
97.478
15.793
1.00
32.00
B


ATOM
2607
C
VAL
B
27
66.054
97.946
17.292
1.00
26.09
B


ATOM
2608
O
VAL
B
27
65.961
98.921
18.029
1.00
28.74
B


ATOM
2609
N
ILE
B
28
65.230
96.913
17.349
1.00
28.74
B


ATOM
2610
CA
ILE
B
28
64.126
96.780
18.294
1.00
34.25
B


ATOM
2611
CB
ILE
B
28
62.802
96.400
17.552
1.00
38.10
B


ATOM
2612
CG2
ILE
B
28
61.752
95.929
18.516
1.00
49.78
B


ATOM
2613
CG1
ILE
B
28
62.260
97.598
16.808
1.00
46.71
B


ATOM
2614
CD1
ILE
B
28
62.252
98.826
17.612
1.00
40.93
B


ATOM
2615
C
ILE
B
28
64.491
95.619
19.223
1.00
38.36
B


ATOM
2616
O
ILE
B
28
65.063
94.626
18.769
1.00
29.90
B


ATOM
2617
N
ARG
B
29
64.134
95.724
20.503
1.00
30.42
B


ATOM
2618
CA
ARG
B
29
64.470
94.673
21.458
1.00
32.43
B


ATOM
2619
CB
ARG
B
29
65.468
95.242
22.461
1.00
38.54
B


ATOM
2620
CG
ARG
B
29
66.234
94.231
23.273
1.00
56.74
B


ATOM
2621
CD
ARG
B
29
67.479
94.939
23.821
1.00
68.43
B


ATOM
2622
NE
ARG
B
29
68.210
95.573
22.722
1.00
77.31
B


ATOM
2623
CZ
ARG
B
29
69.134
94.960
21.983
1.00
81.82
B


ATOM
2624
NH1
ARG
B
29
69.454
93.693
22.237
1.00
82.00
B


ATOM
2625
NH2
ARG
B
29
69.723
95.603
20.976
1.00
82.81
B


ATOM
2626
C
ARG
B
29
63.222
94.132
22.168
1.00
26.06
B


ATOM
2627
O
ARG
B
29
62.432
94.884
22.704
1.00
20.11
B


ATOM
2628
N
TYR
B
30
63.032
92.823
22.133
1.00
22.95
B


ATOM
2629
CA
TYR
B
30
61.886
92.206
22.787
1.00
31.30
B


ATOM
2630
CB
TYR
B
30
61.299
91.096
21.905
1.00
28.61
B


ATOM
2631
CG
TYR
B
30
60.647
91.547
20.605
1.00
29.37
B


ATOM
2632
CD1
TYR
B
30
61.389
92.135
19.579
1.00
24.77
B


ATOM
2633
CE1
TYR
B
30
60.781
92.553
18.387
1.00
19.11
B


ATOM
2634
CD2
TYR
B
30
59.282
91.378
20.403
1.00
29.03
B


ATOM
2635
CE2
TYR
B
30
58.674
91.785
19.215
1.00
26.35
B


ATOM
2636
CZ
TYR
B
30
59.429
92.379
18.216
1.00
23.41
B


ATOM
2637
OH
TYR
B
30
58.793
92.836
17.083
1.00
26.05
B


ATOM
2638
C
TYR
B
30
62.340
91.579
24.126
1.00
28.93
B


ATOM
2639
O
TYR
B
30
63.497
91.168
24.283
1.00
24.49
B


ATOM
2640
N
SER
B
31
61.418
91.488
25.067
1.00
26.50
B


ATOM
2641
CA
SER
B
31
61.697
90.903
26.369
1.00
24.95
B


ATOM
2642
CB
SER
B
31
62.304
91.946
27.308
1.00
21.32
B


ATOM
2643
OG
SER
B
31
61.426
93.028
27.483
1.00
26.55
B


ATOM
2644
C
SER
B
31
60.388
90.378
26.954
1.00
29.25
B


ATOM
2645
O
SER
B
31
59.287
90.780
26.529
1.00
22.45
B


ATOM
2646
N
CYS
B
32
60.515
89.484
27.930
1.00
27.99
B


ATOM
2647
CA
CYS
B
32
59.364
88.868
28.606
1.00
32.41
B


ATOM
2648
C
CYS
B
32
59.371
89.122
30.121
1.00
38.37
B


ATOM
2649
O
CYS
B
32
60.431
89.311
30.719
1.00
37.82
B


ATOM
2650
CB
CYS
B
32
59.371
87.347
28.400
1.00
30.70
B


ATOM
2651
SG
CYS
B
32
59.450
86.770
26.677
1.00
32.11
B


ATOM
2652
N
SER
B
33
58.178
89.101
30.717
1.00
51.37
B


ATOM
2653
CA
SER
B
33
57.995
89.265
32.163
1.00
56.15
B


ATOM
2654
CB
SER
B
33
56.544
89.013
32.552
1.00
60.18
B


ATOM
2655
OG
SER
B
33
55.675
89.714
31.672
1.00
66.23
B


ATOM
2656
C
SER
B
33
58.859
88.260
32.894
1.00
57.27
B


ATOM
2657
O
SER
B
33
59.535
87.427
32.274
1.00
60.30
B


ATOM
2658
N
GLY
B
34
58.805
88.290
34.219
1.00
53.29
B


ATOM
2659
CA
GLY
B
34
59.662
87.391
34.976
1.00
47.70
B


ATOM
2660
C
GLY
B
34
59.278
85.922
34.987
1.00
44.48
B


ATOM
2661
O
GLY
B
34
60.129
85.033
35.135
1.00
38.34
B


ATOM
2662
N
THR
B
35
57.993
85.660
34.818
1.00
40.51
B


ATOM
2663
CA
THR
B
35
57.524
84.290
34.860
1.00
40.76
B


ATOM
2664
CB
THR
B
35
56.225
84.239
35.644
1.00
41.73
B


ATOM
2665
OG1
THR
B
35
55.258
85.077
35.014
1.00
48.51
B


ATOM
2666
CG2
THR
B
35
56.468
84.761
37.057
1.00
44.89
B


ATOM
2667
C
THR
B
35
57.369
83.691
33.470
1.00
39.51
B


ATOM
2668
O
THR
B
35
56.786
82.613
33.286
1.00
31.97
B


ATOM
2669
N
PHE
B
36
57.924
84.399
32.491
1.00
35.56
B


ATOM
2670
CA
PHE
B
36
57.873
83.955
31.112
1.00
31.01
B


ATOM
2671
CB
PHE
B
36
57.146
84.967
30.250
1.00
33.05
B


ATOM
2672
CG
PHE
B
36
55.673
84.918
30.396
1.00
35.23
B


ATOM
2673
CD1
PHE
B
36
55.040
85.573
31.444
1.00
35.53
B


ATOM
2674
CD2
PHE
B
36
54.909
84.204
29.483
1.00
31.53
B


ATOM
2675
CE1
PHE
B
36
53.663
85.497
31.570
1.00
37.58
B


ATOM
2676
CE2
PHE
B
36
53.549
84.126
29.604
1.00
32.60
B


ATOM
2677
CZ
PHE
B
36
52.918
84.769
30.638
1.00
32.38
B


ATOM
2678
C
PHE
B
36
59.276
83.798
30.577
1.00
32.83
B


ATOM
2679
O
PHE
B
36
60.212
84.347
31.125
1.00
33.40
B


ATOM
2680
N
ARG
B
37
59.427
83.049
29.498
1.00
30.99
B


ATOM
2681
CA
ARG
B
37
60.741
82.896
28.916
1.00
29.69
B


ATOM
2682
CB
ARG
B
37
61.201
81.458
29.083
1.00
30.62
B


ATOM
2683
CG
ARG
B
37
61.442
81.072
30.541
1.00
35.61
B


ATOM
2684
CD
ARG
B
37
62.492
81.992
31.177
1.00
39.39
B


ATOM
2685
NE
ARG
B
37
62.303
82.044
32.629
1.00
49.28
B


ATOM
2686
CZ
ARG
B
37
62.847
81.168
33.451
1.00
47.41
B


ATOM
2687
NH1
ARG
B
37
63.614
80.212
32.947
1.00
53.00
B


ATOM
2688
NH2
ARG
B
37
62.602
81.223
34.751
1.00
44.10
B


ATOM
2689
C
ARG
B
37
60.713
83.280
27.439
1.00
32.05
B


ATOM
2690
O
ARG
B
37
59.809
82.874
26.700
1.00
25.63
B


ATOM
2691
N
LEU
B
38
61.706
84.050
27.011
1.00
23.96
B


ATOM
2692
CA
LEU
B
38
61.802
84.470
25.621
1.00
30.62
B


ATOM
2693
CB
LEU
B
38
62.655
85.738
25.508
1.00
23.25
B


ATOM
2694
CG
LEU
B
38
62.739
86.365
24.105
1.00
28.16
B


ATOM
2695
CD1
LEU
B
38
61.376
86.948
23.659
1.00
20.01
B


ATOM
2696
CD2
LEU
B
38
63.753
87.486
24.132
1.00
32.19
B


ATOM
2697
C
LEU
B
38
62.386
83.361
24.745
1.00
27.63
B


ATOM
2698
O
LEU
B
38
63.447
82.793
25.036
1.00
27.98
B


ATOM
2699
N
ILE
B
39
61.651
83.017
23.691
1.00
30.18
B


ATOM
2700
CA
ILE
B
39
62.073
81.983
22.743
1.00
27.37
B


ATOM
2701
CB
ILE
B
39
60.940
80.959
22.492
1.00
30.75
B


ATOM
2702
CG2
ILE
B
39
61.438
79.878
21.538
1.00
29.27
B


ATOM
2703
CG1
ILE
B
39
60.455
80.359
23.822
1.00
30.90
B


ATOM
2704
CD1
ILE
B
39
61.491
79.535
24.559
1.00
26.44
B


ATOM
2705
C
ILE
B
39
62.403
82.668
21.418
1.00
29.53
B


ATOM
2706
O
ILE
B
39
61.510
83.256
20.797
1.00
24.87
B


ATOM
2707
N
GLY
B
40
63.676
82.609
20.998
1.00
21.97
B


ATOM
2708
CA
GLY
B
40
64.084
83.239
19.751
1.00
23.62
B


ATOM
2709
C
GLY
B
40
64.957
84.450
19.975
1.00
22.85
B


ATOM
2710
O
GLY
B
40
65.065
84.921
21.104
1.00
21.42
B


ATOM
2711
N
GLU
B
41
65.592
84.958
18.925
1.00
27.33
B


ATOM
2712
CA
GLU
B
41
66.472
86.133
19.065
1.00
29.50
B


ATOM
2713
CB
GLU
B
41
67.138
86.471
17.730
1.00
33.78
B


ATOM
2714
CG
GLU
B
41
68.634
86.193
17.721
1.00
52.17
B


ATOM
2715
CD
GLU
B
41
69.411
87.123
18.648
1.00
57.74
B


ATOM
2716
OE1
GLU
B
41
70.414
86.641
19.231
1.00
66.30
B


ATOM
2717
OE2
GLU
B
41
69.034
88.326
18.791
1.00
53.76
B


ATOM
2718
C
GLU
B
41
65.667
87.333
19.541
1.00
30.40
B


ATOM
2719
O
GLU
B
41
64.601
87.619
18.995
1.00
27.22
B


ATOM
2720
N
LYS
B
42
66.197
88.049
20.528
1.00
27.86
B


ATOM
2721
CA
LYS
B
42
65.538
89.215
21.135
1.00
32.03
B


ATOM
2722
CB
LYS
B
42
66.171
89.495
22.504
1.00
37.19
B


ATOM
2723
CG
LYS
B
42
67.610
89.990
22.379
1.00
43.78
B


ATOM
2724
CD
LYS
B
42
68.196
90.536
23.674
1.00
54.68
B


ATOM
2725
CE
LYS
B
42
68.055
89.538
24.812
1.00
62.51
B


ATOM
2726
NZ
LYS
B
42
68.438
88.139
24.443
1.00
69.20
B


ATOM
2727
C
LYS
B
42
65.586
90.530
20.331
1.00
32.32
B


ATOM
2728
O
LYS
B
42
64.771
91.432
20.555
1.00
27.61
B


ATOM
2729
N
SER
B
43
66.547
90.680
19.422
1.00
28.99
B


ATOM
2730
CA
SER
B
43
66.611
91.946
18.672
1.00
33.61
B


ATOM
2731
CB
SER
B
43
68.012
92.565
18.722
1.00
29.97
B


ATOM
2732
OG
SER
B
43
68.478
92.606
20.047
1.00
47.21
B


ATOM
2733
C
SER
B
43
66.273
91.770
17.224
1.00
28.57
B


ATOM
2734
O
SER
B
43
66.765
90.841
16.588
1.00
32.11
B


ATOM
2735
N
LEU
B
44
65.443
92.661
16.697
1.00
26.09
B


ATOM
2736
CA
LEU
B
44
65.127
92.607
15.280
1.00
25.34
B


ATOM
2737
CB
LEU
B
44
63.620
92.609
14.987
1.00
21.75
B


ATOM
2738
CG
LEU
B
44
62.608
91.642
15.593
1.00
33.26
B


ATOM
2739
CD1
LEU
B
44
61.455
91.487
14.626
1.00
24.66
B


ATOM
2740
CD2
LEU
B
44
63.227
90.293
15.976
1.00
19.72
B


ATOM
2741
C
LEU
B
44
65.738
93.863
14.694
1.00
27.39
B


ATOM
2742
O
LEU
B
44
65.759
94.919
15.334
1.00
29.07
B


ATOM
2743
N
LEU
B
45
66.201
93.740
13.456
1.00
26.79
B


ATOM
2744
CA
LEU
B
45
66.854
94.812
12.727
1.00
26.17
B


ATOM
2745
CB
LEU
B
45
68.202
94.303
12.213
1.00
25.36
B


ATOM
2746
CG
LEU
B
45
69.343
95.281
11.950
1.00
35.90
B


ATOM
2747
CD1
LEU
B
45
70.263
94.668
10.905
1.00
31.78
B


ATOM
2748
CD2
LEU
B
45
68.853
96.627
11.519
1.00
39.62
B


ATOM
2749
C
LEU
B
45
66.016
95.198
11.516
1.00
25.11
B


ATOM
2750
O
LEU
B
45
65.568
94.320
10.761
1.00
20.94
B


ATOM
2751
N
CYS
B
46
65.803
96.487
11.298
1.00
24.85
B


ATOM
2752
CA
CYS
B
46
65.057
96.887
10.097
1.00
22.87
B


ATOM
2753
C
CYS
B
46
66.106
96.897
8.976
1.00
29.24
B


ATOM
2754
O
CYS
B
46
67.099
97.601
9.051
1.00
28.50
B


ATOM
2755
CB
CYS
B
46
64.446
98.284
10.251
1.00
26.50
B


ATOM
2756
SG
CYS
B
46
63.677
98.900
8.705
1.00
27.31
B


ATOM
2757
N
ILE
B
47
65.877
96.106
7.943
1.00
24.65
B


ATOM
2758
CA
ILE
B
47
66.817
95.991
6.842
1.00
28.64
B


ATOM
2759
CB
ILE
B
47
67.412
94.572
6.871
1.00
30.17
B


ATOM
2760
CG2
ILE
B
47
68.114
94.258
5.601
1.00
42.28
B


ATOM
2761
CG1
ILE
B
47
68.341
94.458
8.070
1.00
40.16
B


ATOM
2762
CD1
ILE
B
47
69.121
93.190
8.107
1.00
53.27
B


ATOM
2763
C
ILE
B
47
66.098
96.188
5.504
1.00
28.32
B


ATOM
2764
O
ILE
B
47
64.874
96.332
5.466
1.00
24.72
B


ATOM
2765
N
THR
B
48
66.853
96.238
4.413
1.00
24.30
B


ATOM
2766
CA
THR
B
48
66.236
96.267
3.091
1.00
27.24
B


ATOM
2767
CB
THR
B
48
66.215
97.672
2.405
1.00
31.04
B


ATOM
2768
OG1
THR
B
48
65.632
97.534
1.090
1.00
26.58
B


ATOM
2769
CG2
THR
B
48
67.627
98.250
2.271
1.00
23.59
B


ATOM
2770
C
THR
B
48
67.048
95.280
2.254
1.00
26.55
B


ATOM
2771
O
THR
B
48
68.276
95.371
2.173
1.00
27.30
B


ATOM
2772
N
LYS
B
49
66.370
94.295
1.688
1.00
24.39
B


ATOM
2773
CA
LYS
B
49
67.031
93.309
0.837
1.00
25.65
B


ATOM
2774
CB
LYS
B
49
66.323
91.943
0.940
1.00
27.44
B


ATOM
2775
CG
LYS
B
49
66.358
91.351
2.368
1.00
36.21
B


ATOM
2776
CD
LYS
B
49
67.171
90.103
2.479
1.00
40.52
B


ATOM
2777
CE
LYS
B
49
68.570
90.294
2.025
1.00
41.63
B


ATOM
2778
NZ
LYS
B
49
69.314
89.069
2.403
1.00
46.47
B


ATOM
2779
C
LYS
B
49
67.041
93.752
−0.632
1.00
33.01
B


ATOM
2780
O
LYS
B
49
67.987
93.450
−1.353
1.00
28.70
B


ATOM
2781
N
ASP
B
50
65.994
94.451
−1.083
1.00
26.02
B


ATOM
2782
CA
ASP
B
50
65.933
94.859
−2.495
1.00
29.77
B


ATOM
2783
CB
ASP
B
50
64.575
94.503
−3.108
1.00
28.99
B


ATOM
2784
CG
ASP
B
50
63.439
95.221
−2.440
1.00
26.54
B


ATOM
2785
OD1
ASP
B
50
63.720
96.043
−1.562
1.00
25.74
B


ATOM
2786
OD2
ASP
B
50
62.262
94.969
−2.779
1.00
32.42
B


ATOM
2787
C
ASP
B
50
66.200
96.327
−2.744
1.00
30.62
B


ATOM
2788
O
ASP
B
50
66.037
96.794
−3.858
1.00
24.13
B


ATOM
2789
N
LYS
B
51
66.610
97.050
−1.709
1.00
27.94
B


ATOM
2790
CA
LYS
B
51
66.889
98.473
−1.824
1.00
31.18
B


ATOM
2791
CB
LYS
B
51
68.014
98.742
−2.819
1.00
34.46
B


ATOM
2792
CG
LYS
B
51
69.381
98.355
−2.297
1.00
41.85
B


ATOM
2793
CD
LYS
B
51
70.447
98.960
−3.175
1.00
49.97
B


ATOM
2794
CE
LYS
B
51
71.826
98.869
−2.564
1.00
59.90
B


ATOM
2795
NZ
LYS
B
51
72.754
99.713
−3.366
1.00
67.70
B


ATOM
2796
C
LYS
B
51
65.666
99.305
−2.186
1.00
30.87
B


ATOM
2797
O
LYS
B
51
65.786
100.430
−2.634
1.00
28.68
B


ATOM
2798
N
VAL
B
52
64.483
98.748
−1.992
1.00
26.95
B


ATOM
2799
CA
VAL
B
52
63.263
99.501
−2.231
1.00
26.37
B


ATOM
2800
CB
VAL
B
52
62.456
98.945
−3.402
1.00
27.69
B


ATOM
2801
CG1
VAL
B
52
61.123
99.695
−3.511
1.00
32.70
B


ATOM
2802
CG2
VAL
B
52
63.236
99.113
−4.679
1.00
32.13
B


ATOM
2803
C
VAL
B
52
62.375
99.437
−1.000
1.00
30.79
B


ATOM
2804
O
VAL
B
52
61.949
100.461
−0.491
1.00
26.76
B


ATOM
2805
N
ASP
B
53
62.102
98.232
−0.512
1.00
26.86
B


ATOM
2806
CA
ASP
B
53
61.210
98.074
0.642
1.00
26.94
B


ATOM
2807
CB
ASP
B
53
60.156
96.999
0.318
1.00
42.02
B


ATOM
2808
CG
ASP
B
53
59.285
97.356
−0.871
1.00
53.53
B


ATOM
2809
OD1
ASP
B
53
58.878
98.540
−0.943
1.00
63.13
B


ATOM
2810
OD2
ASP
B
53
58.964
96.476
−1.727
1.00
64.13
B


ATOM
2811
C
ASP
B
53
62.006
97.651
1.888
1.00
25.00
B


ATOM
2812
O
ASP
B
53
63.063
97.038
1.773
1.00
22.23
B


ATOM
2813
N
GLY
B
54
61.490
97.959
3.072
1.00
20.64
B


ATOM
2814
CA
GLY
B
54
62.170
97.578
4.293
1.00
24.10
B


ATOM
2815
C
GLY
B
54
61.441
96.397
4.919
1.00
25.17
B


ATOM
2816
O
GLY
B
54
60.237
96.266
4.728
1.00
25.23
B


ATOM
2817
N
THR
B
55
62.169
95.517
5.605
1.00
23.87
B


ATOM
2818
CA
THR
B
55
61.589
94.373
6.318
1.00
20.36
B


ATOM
2819
CB
THR
B
55
61.679
93.051
5.518
1.00
27.74
B


ATOM
2820
OG1
THR
B
55
61.052
92.004
6.267
1.00
24.11
B


ATOM
2821
CG2
THR
B
55
63.151
92.669
5.212
1.00
21.92
B


ATOM
2822
C
THR
B
55
62.371
94.156
7.600
1.00
22.54
B


ATOM
2823
O
THR
B
55
63.535
94.526
7.675
1.00
24.57
B


ATOM
2824
N
TRP
B
56
61.743
93.589
8.624
1.00
23.11
B


ATOM
2825
CA
TRP
B
56
62.513
93.285
9.827
1.00
25.54
B


ATOM
2826
CB
TRP
B
56
61.580
92.954
10.988
1.00
23.18
B


ATOM
2827
CG
TRP
B
56
60.874
94.202
11.420
1.00
19.51
B


ATOM
2828
CD2
TRP
B
56
61.456
95.282
12.147
1.00
21.91
B


ATOM
2829
CE2
TRP
B
56
60.473
96.302
12.247
1.00
26.89
B


ATOM
2830
CE3
TRP
B
56
62.722
95.491
12.729
1.00
25.30
B


ATOM
2831
CD1
TRP
B
56
59.595
94.578
11.119
1.00
21.93
B


ATOM
2832
NE1
TRP
B
56
59.343
95.849
11.617
1.00
21.49
B


ATOM
2833
CZ2
TRP
B
56
60.713
97.521
12.895
1.00
26.71
B


ATOM
2834
CZ3
TRP
B
56
62.963
96.706
13.380
1.00
29.35
B


ATOM
2835
CH2
TRP
B
56
61.957
97.706
13.451
1.00
28.96
B


ATOM
2836
C
TRP
B
56
63.335
92.075
9.384
1.00
28.11
B


ATOM
2837
O
TRP
B
56
62.882
91.296
8.535
1.00
23.54
B


ATOM
2838
N
ASP
B
57
64.535
91.909
9.926
1.00
20.44
B


ATOM
2839
CA
ASP
B
57
65.401
90.812
9.470
1.00
25.81
B


ATOM
2840
CB
ASP
B
57
66.885
91.113
9.761
1.00
30.15
B


ATOM
2841
CG
ASP
B
57
67.234
91.045
11.255
1.00
35.84
B


ATOM
2842
OD1
ASP
B
57
66.319
91.161
12.102
1.00
23.31
B


ATOM
2843
OD2
ASP
B
57
68.443
90.889
11.581
1.00
41.06
B


ATOM
2844
C
ASP
B
57
65.058
89.433
9.963
1.00
26.11
B


ATOM
2845
O
ASP
B
57
65.634
88.455
9.486
1.00
28.90
B


ATOM
2846
N
LYS
B
58
64.114
89.336
10.896
1.00
29.91
B


ATOM
2847
CA
LYS
B
58
63.687
88.032
11.411
1.00
27.97
B


ATOM
2848
CB
LYS
B
58
64.697
87.491
12.443
1.00
30.61
B


ATOM
2849
CG
LYS
B
58
64.884
88.378
13.650
1.00
38.23
B


ATOM
2850
CD
LYS
B
58
65.966
87.831
14.618
1.00
41.02
B


ATOM
2851
CE
LYS
B
58
67.406
88.063
14.115
1.00
37.73
B


ATOM
2852
NZ
LYS
B
58
67.830
89.511
14.119
1.00
29.19
B


ATOM
2853
C
LYS
B
58
62.319
88.105
12.045
1.00
22.71
B


ATOM
2854
O
LYS
B
58
61.795
89.188
12.311
1.00
26.33
B


ATOM
2855
N
PRO
B
59
61.700
86.947
12.292
1.00
25.99
B


ATOM
2856
CD
PRO
B
59
62.084
85.584
11.885
1.00
24.09
B


ATOM
2857
CA
PRO
B
59
60.367
86.972
12.919
1.00
22.11
B


ATOM
2858
CB
PRO
B
59
59.902
85.515
12.845
1.00
26.86
B


ATOM
2859
CG
PRO
B
59
60.730
84.913
11.746
1.00
29.91
B


ATOM
2860
C
PRO
B
59
60.509
87.419
14.369
1.00
30.21
B


ATOM
2861
O
PRO
B
59
61.593
87.307
14.968
1.00
28.15
B


ATOM
2862
N
ALA
B
60
59.433
87.924
14.951
1.00
25.43
B


ATOM
2863
CA
ALA
B
60
59.516
88.330
16.339
1.00
26.37
B


ATOM
2864
CB
ALA
B
60
58.260
89.089
16.742
1.00
26.63
B


ATOM
2865
C
ALA
B
60
59.644
87.085
17.223
1.00
21.91
B


ATOM
2866
O
ALA
B
60
59.104
86.068
16.914
1.00
22.47
B


ATOM
2867
N
PRO
B
61
60.358
87.173
18.350
1.00
23.37
B


ATOM
2868
CD
PRO
B
61
61.134
88.320
18.870
1.00
22.24
B


ATOM
2869
CA
PRO
B
61
60.476
86.007
19.227
1.00
19.99
B


ATOM
2870
CB
PRO
B
61
61.650
86.386
20.130
1.00
21.18
B


ATOM
2871
CG
PRO
B
61
61.458
87.876
20.314
1.00
22.73
B


ATOM
2872
C
PRO
B
61
59.151
85.933
20.024
1.00
27.42
B


ATOM
2873
O
PRO
B
61
58.314
86.831
19.903
1.00
27.48
B


ATOM
2874
N
LYS
B
62
58.960
84.893
20.834
1.00
21.73
B


ATOM
2875
CA
LYS
B
62
57.737
84.770
21.648
1.00
26.90
B


ATOM
2876
CB
LYS
B
62
56.880
83.576
21.187
1.00
32.90
B


ATOM
2877
CG
LYS
B
62
56.318
83.743
19.779
1.00
39.12
B


ATOM
2878
CD
LYS
B
62
55.638
82.502
19.258
1.00
48.29
B


ATOM
2879
CE
LYS
B
62
55.142
82.741
17.828
1.00
54.55
B


ATOM
2880
NZ
LYS
B
62
54.620
81.478
17.213
1.00
59.87
B


ATOM
2881
C
LYS
B
62
58.050
84.601
23.133
1.00
30.68
B


ATOM
2882
O
LYS
B
62
59.190
84.287
23.528
1.00
24.62
B


ATOM
2883
N
CYS
B
63
57.039
84.843
23.958
1.00
25.15
B


ATOM
2884
CA
CYS
B
63
57.171
84.675
25.405
1.00
27.27
B


ATOM
2885
C
CYS
B
63
56.314
83.481
25.792
1.00
30.43
B


ATOM
2886
O
CYS
B
63
55.111
83.454
25.515
1.00
30.82
B


ATOM
2887
CB
CYS
B
63
56.646
85.903
26.149
1.00
25.60
B


ATOM
2888
SG
CYS
B
63
57.682
87.384
25.922
1.00
31.81
B


ATOM
2889
N
GLU
B
64
56.934
82.486
26.414
1.00
29.14
B


ATOM
2890
CA
GLU
B
64
56.220
81.297
26.871
1.00
27.87
B


ATOM
2891
CB
GLU
B
64
56.938
80.043
26.393
1.00
22.54
B


ATOM
2892
CG
GLU
B
64
56.839
79.836
24.884
1.00
30.23
B


ATOM
2893
CD
GLU
B
64
57.354
78.472
24.471
1.00
25.42
B


ATOM
2894
OE1
GLU
B
64
58.146
77.879
25.221
1.00
28.63
B


ATOM
2895
OE2
GLU
B
64
56.993
77.994
23.396
1.00
24.45
B


ATOM
2896
C
GLU
B
64
56.172
81.301
28.394
1.00
27.43
B


ATOM
2897
O
GLU
B
64
57.189
81.564
29.036
1.00
28.45
B


ATOM
2898
N
TYR
B
65
55.003
81.039
28.981
1.00
30.82
B


ATOM
2899
CA
TYR
B
65
54.898
81.016
30.449
1.00
26.71
B


ATOM
2900
CB
TYR
B
65
53.474
80.638
30.881
1.00
24.67
B


ATOM
2901
CG
TYR
B
65
53.222
80.754
32.382
1.00
30.61
B


ATOM
2902
CD1
TYR
B
65
53.558
81.915
33.078
1.00
37.93
B


ATOM
2903
CE1
TYR
B
65
53.369
82.013
34.464
1.00
37.10
B


ATOM
2904
CD2
TYR
B
65
52.681
79.689
33.102
1.00
29.91
B


ATOM
2905
CE2
TYR
B
65
52.481
79.765
34.477
1.00
32.04
B


ATOM
2906
CZ
TYR
B
65
52.829
80.927
35.149
1.00
38.91
B


ATOM
2907
OH
TYR
B
65
52.653
81.014
36.510
1.00
40.82
B


ATOM
2908
C
TYR
B
65
55.931
79.969
30.908
1.00
26.37
B


ATOM
2909
O
TYR
B
65
56.022
78.891
30.339
1.00
27.39
B


ATOM
2910
N
PHE
B
66
56.722
80.290
31.924
1.00
30.93
B


ATOM
2911
CA
PHE
B
66
57.770
79.381
32.362
1.00
29.14
B


ATOM
2912
CB
PHE
B
66
58.611
80.060
33.455
1.00
27.56
B


ATOM
2913
CG
PHE
B
66
59.743
79.210
33.998
1.00
28.67
B


ATOM
2914
CD1
PHE
B
66
60.632
78.559
33.139
1.00
33.68
B


ATOM
2915
CD2
PHE
B
66
59.907
79.055
35.371
1.00
30.48
B


ATOM
2916
CE1
PHE
B
66
61.669
77.763
33.648
1.00
35.23
B


ATOM
2917
CE2
PHE
B
66
60.931
78.267
35.895
1.00
26.93
B


ATOM
2918
CZ
PHE
B
66
61.816
77.618
35.041
1.00
31.25
B


ATOM
2919
C
PHE
B
66
57.283
78.013
32.844
1.00
32.09
B


ATOM
2920
O
PHE
B
66
56.382
77.924
33.671
1.00
31.84
B


ATOM
2921
N
ASN
B
67
57.882
76.956
32.312
1.00
28.91
B


ATOM
2922
CA
ASN
B
67
57.553
75.591
32.727
1.00
31.14
B


ATOM
2923
CB
ASN
B
67
57.124
74.763
31.524
1.00
32.40
B


ATOM
2924
CG
ASN
B
67
56.635
73.368
31.903
1.00
37.56
B


ATOM
2925
OD1
ASN
B
67
57.193
72.701
32.784
1.00
33.77
B


ATOM
2926
ND2
ASN
B
67
55.589
72.914
31.218
1.00
40.31
B


ATOM
2927
C
ASN
B
67
58.844
75.009
33.318
1.00
30.31
B


ATOM
2928
O
ASN
B
67
59.719
74.551
32.577
1.00
26.39
B


ATOM
2929
N
LYS
B
68
58.960
75.038
34.644
1.00
28.27
B


ATOM
2930
CA
LYS
B
68
60.148
74.535
35.329
1.00
29.00
B


ATOM
2931
CB
LYS
B
68
60.041
74.792
36.843
1.00
35.08
B


ATOM
2932
CG
LYS
B
68
58.984
73.906
37.518
1.00
41.56
B


ATOM
2933
CD
LYS
B
68
59.179
73.778
39.031
1.00
52.33
B


ATOM
2934
CE
LYS
B
68
58.907
75.077
39.764
1.00
54.82
B


ATOM
2935
NZ
LYS
B
68
58.896
74.891
41.250
1.00
58.88
B


ATOM
2936
C
LYS
B
68
60.398
73.039
35.087
1.00
32.74
B


ATOM
2937
O
LYS
B
68
61.507
72.556
35.318
1.00
32.92
B


ATOM
2938
N
TYR
B
69
59.389
72.305
34.611
1.00
33.19
B


ATOM
2939
CA
TYR
B
69
59.559
70.867
34.354
1.00
25.62
B


ATOM
2940
CB
TYR
B
69
58.259
70.096
34.687
1.00
30.97
B


ATOM
2941
CG
TYR
B
69
57.801
70.349
36.107
1.00
30.82
B


ATOM
2942
CD1
TYR
B
69
56.774
71.266
36.378
1.00
28.48
B


ATOM
2943
CE1
TYR
B
69
56.444
71.623
37.697
1.00
31.73
B


ATOM
2944
CD2
TYR
B
69
58.486
69.776
37.192
1.00
31.64
B


ATOM
2945
CE2
TYR
B
69
58.174
70.118
38.507
1.00
38.99
B


ATOM
2946
CZ
TYR
B
69
57.153
71.053
38.756
1.00
39.94
B


ATOM
2947
OH
TYR
B
69
56.901
71.467
40.048
1.00
41.68
B


ATOM
2948
C
TYR
B
69
60.029
70.516
32.955
1.00
31.61
B


ATOM
2949
O
TYR
B
69
60.549
69.414
32.722
1.00
31.53
B


ATOM
2950
N
SER
B
70
59.889
71.447
32.017
1.00
29.13
B


ATOM
2951
CA
SER
B
70
60.328
71.162
30.649
1.00
31.79
B


ATOM
2952
CB
SER
B
70
59.926
72.310
29.708
1.00
27.84
B


ATOM
2953
OG
SER
B
70
58.524
72.534
29.725
1.00
38.42
B


ATOM
2954
C
SER
B
70
61.849
70.949
30.544
1.00
34.24
B


ATOM
2955
O
SER
B
70
62.624
71.534
31.296
1.00
31.38
B


ATOM
2956
N
SER
B
71
62.261
70.097
29.613
1.00
33.64
B


ATOM
2957
CA
SER
B
71
63.676
69.832
29.351
1.00
36.09
B


ATOM
2958
CB
SER
B
71
64.265
68.809
30.324
1.00
39.34
B


ATOM
2959
OG
SER
B
71
63.823
67.519
30.007
1.00
41.01
B


ATOM
2960
C
SER
B
71
63.790
69.308
27.907
1.00
41.17
B


ATOM
2961
O
SER
B
71
62.959
68.520
27.432
1.00
41.90
B


ATOM
2962
N
CYS
B
72
64.805
69.766
27.195
1.00
32.35
B


ATOM
2963
CA
CYS
B
72
64.970
69.351
25.816
1.00
36.35
B


ATOM
2964
C
CYS
B
72
66.139
68.393
25.722
1.00
36.24
B


ATOM
2965
O
CYS
B
72
67.089
68.472
26.503
1.00
34.41
B


ATOM
2966
CB
CYS
B
72
65.205
70.586
24.920
1.00
26.09
B


ATOM
2967
SG
CYS
B
72
63.836
71.795
24.962
1.00
32.64
B


ATOM
2968
N
PRO
B
73
66.069
67.453
24.779
1.00
35.65
B


ATOM
2969
CD
PRO
B
73
64.958
67.148
23.867
1.00
38.10
B


ATOM
2970
CA
PRO
B
73
67.157
66.494
24.617
1.00
35.61
B


ATOM
2971
CB
PRO
B
73
66.567
65.461
23.661
1.00
35.75
B


ATOM
2972
CG
PRO
B
73
65.662
66.314
22.806
1.00
40.23
B


ATOM
2973
C
PRO
B
73
68.355
67.203
24.011
1.00
37.18
B


ATOM
2974
O
PRO
B
73
68.225
68.277
23.427
1.00
31.86
B


ATOM
2975
N
GLU
B
74
69.521
66.595
24.151
1.00
30.08
B


ATOM
2976
CA
GLU
B
74
70.726
67.168
23.594
1.00
36.66
B


ATOM
2977
CB
GLU
B
74
71.904
66.221
23.830
1.00
40.69
B


ATOM
2978
CG
GLU
B
74
73.123
66.500
22.972
1.00
55.61
B


ATOM
2979
CD
GLU
B
74
74.357
66.847
23.791
1.00
65.33
B


ATOM
2980
OE1
GLU
B
74
75.476
66.535
23.311
1.00
68.86
B


ATOM
2981
OE2
GLU
B
74
74.214
67.432
24.899
1.00
67.56
B


ATOM
2982
C
GLU
B
74
70.526
67.411
22.099
1.00
31.83
B


ATOM
2983
O
GLU
B
74
70.179
66.517
21.347
1.00
34.92
B


ATOM
2984
N
PRO
B
75
70.743
68.639
21.648
1.00
29.85
B


ATOM
2985
CD
PRO
B
75
71.019
69.851
22.431
1.00
29.21
B


ATOM
2986
CA
PRO
B
75
70.573
68.944
20.227
1.00
31.16
B


ATOM
2987
CB
PRO
B
75
70.349
70.441
20.235
1.00
30.77
B


ATOM
2988
CG
PRO
B
75
71.310
70.872
21.362
1.00
28.27
B


ATOM
2989
C
PRO
B
75
71.855
68.552
19.507
1.00
37.41
B


ATOM
2990
O
PRO
B
75
72.949
68.998
19.877
1.00
35.98
B


ATOM
2991
N
ILE
B
76
71.728
67.726
18.474
1.00
37.41
B


ATOM
2992
CA
ILE
B
76
72.904
67.277
17.757
1.00
41.40
B


ATOM
2993
CB
ILE
B
76
73.069
65.755
17.912
1.00
47.80
B


ATOM
2994
CG2
ILE
B
76
74.385
65.302
17.272
1.00
48.99
B


ATOM
2995
CG1
ILE
B
76
73.078
65.396
19.404
1.00
48.44
B


ATOM
2996
CD1
ILE
B
76
72.926
63.918
19.671
1.00
55.08
B


ATOM
2997
C
ILE
B
76
72.874
67.654
16.294
1.00
38.62
B


ATOM
2998
O
ILE
B
76
71.874
67.480
15.625
1.00
39.69
B


ATOM
2999
N
VAL
B
77
73.984
68.195
15.813
1.00
36.89
B


ATOM
3000
CA
VAL
B
77
74.107
68.610
14.433
1.00
38.30
B


ATOM
3001
CB
VAL
B
77
74.290
70.119
14.331
1.00
39.19
B


ATOM
3002
CG1
VAL
B
77
74.483
70.519
12.871
1.00
39.16
B


ATOM
3003
CG2
VAL
B
77
73.081
70.826
14.946
1.00
40.99
B


ATOM
3004
C
VAL
B
77
75.307
67.939
13.761
1.00
40.36
B


ATOM
3005
O
VAL
B
77
76.457
68.351
13.948
1.00
41.17
B


ATOM
3006
N
PRO
B
78
75.057
66.893
12.966
1.00
40.75
B


ATOM
3007
CD
PRO
B
78
73.785
66.267
12.590
1.00
37.39
B


ATOM
3008
CA
PRO
B
78
76.183
66.228
12.303
1.00
40.96
B


ATOM
3009
CB
PRO
B
78
75.506
65.195
11.412
1.00
41.91
B


ATOM
3010
CG
PRO
B
78
74.119
65.745
11.229
1.00
45.49
B


ATOM
3011
C
PRO
B
78
76.997
67.232
11.525
1.00
37.80
B


ATOM
3012
O
PRO
B
78
76.447
68.140
10.913
1.00
37.38
B


ATOM
3013
N
GLY
B
79
78.312
67.083
11.590
1.00
32.94
B


ATOM
3014
CA
GLY
B
79
79.186
68.002
10.900
1.00
36.40
B


ATOM
3015
C
GLY
B
79
79.427
69.261
11.712
1.00
34.31
B


ATOM
3016
O
GLY
B
79
80.209
70.109
11.304
1.00
42.13
B


ATOM
3017
N
GLY
B
80
78.760
69.404
12.855
1.00
36.96
B


ATOM
3018
CA
GLY
B
80
78.960
70.608
13.664
1.00
39.25
B


ATOM
3019
C
GLY
B
80
79.072
70.348
15.164
1.00
35.08
B


ATOM
3020
O
GLY
B
80
78.994
69.202
15.609
1.00
40.28
B


ATOM
3021
N
TYR
B
81
79.225
71.410
15.953
1.00
37.65
B


ATOM
3022
CA
TYR
B
81
79.345
71.271
17.408
1.00
33.04
B


ATOM
3023
CB
TYR
B
81
80.800
71.019
17.799
1.00
29.39
B


ATOM
3024
CG
TYR
B
81
81.745
72.112
17.331
1.00
31.26
B


ATOM
3025
CD1
TYR
B
81
82.169
73.116
18.199
1.00
28.35
B


ATOM
3026
CE1
TYR
B
81
82.992
74.143
17.766
1.00
28.17
B


ATOM
3027
CD2
TYR
B
81
82.181
72.163
16.004
1.00
28.62
B


ATOM
3028
CE2
TYR
B
81
83.006
73.188
15.559
1.00
31.47
B


ATOM
3029
CZ
TYR
B
81
83.401
74.174
16.449
1.00
30.40
B


ATOM
3030
OH
TYR
B
81
84.172
75.207
16.012
1.00
32.87
B


ATOM
3031
C
TYR
B
81
78.847
72.536
18.095
1.00
33.96
B


ATOM
3032
O
TYR
B
81
78.696
73.581
17.454
1.00
30.79
B


ATOM
3033
N
LYS
B
82
78.600
72.431
19.398
1.00
23.56
B


ATOM
3034
CA
LYS
B
82
78.114
73.557
20.195
1.00
29.94
B


ATOM
3035
CB
LYS
B
82
77.348
73.034
21.428
1.00
25.29
B


ATOM
3036
CG
LYS
B
82
76.140
72.144
21.057
1.00
27.67
B


ATOM
3037
CD
LYS
B
82
75.335
71.652
22.266
1.00
33.47
B


ATOM
3038
CE
LYS
B
82
76.215
71.041
23.365
1.00
44.83
B


ATOM
3039
NZ
LYS
B
82
77.091
69.944
22.837
1.00
45.24
B


ATOM
3040
C
LYS
B
82
79.205
74.526
20.629
1.00
29.85
B


ATOM
3041
O
LYS
B
82
80.270
74.110
21.099
1.00
31.44
B


ATOM
3042
N
ILE
B
83
78.980
75.822
20.428
1.00
25.93
B


ATOM
3043
CA
ILE
B
83
79.959
76.795
20.887
1.00
21.30
B


ATOM
3044
CB
ILE
B
83
80.449
77.771
19.800
1.00
29.03
B


ATOM
3045
CG2
ILE
B
83
81.296
77.004
18.799
1.00
23.68
B


ATOM
3046
CG1
ILE
B
83
79.278
78.514
19.155
1.00
24.89
B


ATOM
3047
CD1
ILE
B
83
79.722
79.571
18.148
1.00
27.44
B


ATOM
3048
C
ILE
B
83
79.362
77.581
22.027
1.00
26.62
B


ATOM
3049
O
ILE
B
83
80.038
78.408
22.636
1.00
32.15
B


ATOM
3050
N
ARG
B
84
78.092
77.330
22.324
1.00
24.60
B


ATOM
3051
CA
ARG
B
84
77.471
77.981
23.469
1.00
25.74
B


ATOM
3052
CB
ARG
B
84
77.047
79.396
23.131
1.00
34.80
B


ATOM
3053
CG
ARG
B
84
76.583
80.160
24.348
1.00
45.89
B


ATOM
3054
CD
ARG
B
84
76.518
81.629
24.027
1.00
56.25
B


ATOM
3055
NE
ARG
B
84
77.801
82.302
24.217
1.00
62.31
B


ATOM
3056
CZ
ARG
B
84
78.276
82.707
25.397
1.00
64.61
B


ATOM
3057
NH1
ARG
B
84
77.581
82.496
26.512
1.00
65.21
B


ATOM
3058
NH2
ARG
B
84
79.421
83.386
25.454
1.00
61.85
B


ATOM
3059
C
ARG
B
84
76.278
77.199
23.984
1.00
27.61
B


ATOM
3060
O
ARG
B
84
75.487
76.687
23.189
1.00
24.27
B


ATOM
3061
N
GLY
B
85
76.166
77.105
25.314
1.00
25.11
B


ATOM
3062
CA
GLY
B
85
75.074
76.374
25.959
1.00
23.64
B


ATOM
3063
C
GLY
B
85
75.394
74.877
26.115
1.00
32.13
B


ATOM
3064
O
GLY
B
85
75.961
74.257
25.200
1.00
25.66
B


ATOM
3065
N
SER
B
86
75.094
74.293
27.281
1.00
24.97
B


ATOM
3066
CA
SER
B
86
75.347
72.859
27.473
1.00
34.32
B


ATOM
3067
CB
SER
B
86
76.755
72.588
28.034
1.00
31.54
B


ATOM
3068
OG
SER
B
86
76.964
73.322
29.223
1.00
42.05
B


ATOM
3069
C
SER
B
86
74.315
72.304
28.415
1.00
27.65
B


ATOM
3070
O
SER
B
86
73.536
73.064
28.979
1.00
24.75
B


ATOM
3071
N
THR
B
87
74.294
70.982
28.573
1.00
27.03
B


ATOM
3072
CA
THR
B
87
73.325
70.314
29.454
1.00
29.87
B


ATOM
3073
CB
THR
B
87
73.532
68.806
29.450
1.00
34.20
B


ATOM
3074
OG1
THR
B
87
73.876
68.395
28.125
1.00
54.47
B


ATOM
3075
CG2
THR
B
87
72.263
68.107
29.856
1.00
36.26
B


ATOM
3076
C
THR
B
87
73.420
70.767
30.903
1.00
30.09
B


ATOM
3077
O
THR
B
87
74.501
71.113
31.381
1.00
30.94
B


ATOM
3078
N
PRO
B
88
72.293
70.771
31.626
1.00
26.03
B


ATOM
3079
CD
PRO
B
88
72.370
71.088
33.060
1.00
30.64
B


ATOM
3080
CA
PRO
B
88
70.921
70.406
31.240
1.00
33.13
B


ATOM
3081
CB
PRO
B
88
70.225
70.211
32.585
1.00
28.86
B


ATOM
3082
CG
PRO
B
88
70.903
71.248
33.440
1.00
31.91
B


ATOM
3083
C
PRO
B
88
70.228
71.482
30.388
1.00
31.83
B


ATOM
3084
O
PRO
B
88
70.495
72.668
30.535
1.00
29.33
B


ATOM
3085
N
TYR
B
89
69.340
71.059
29.503
1.00
27.84
B


ATOM
3086
CA
TYR
B
89
68.613
71.990
28.641
1.00
29.55
B


ATOM
3087
CB
TYR
B
89
68.553
71.414
27.239
1.00
27.73
B


ATOM
3088
CG
TYR
B
89
69.938
71.078
26.722
1.00
29.87
B


ATOM
3089
CD1
TYR
B
89
70.326
69.759
26.501
1.00
33.12
B


ATOM
3090
CE1
TYR
B
89
71.605
69.456
26.036
1.00
26.70
B


ATOM
3091
CD2
TYR
B
89
70.865
72.086
26.467
1.00
32.19
B


ATOM
3092
CE2
TYR
B
89
72.134
71.795
26.007
1.00
28.60
B


ATOM
3093
CZ
TYR
B
89
72.492
70.482
25.802
1.00
27.81
B


ATOM
3094
OH
TYR
B
89
73.770
70.211
25.415
1.00
32.86
B


ATOM
3095
C
TYR
B
89
67.212
72.242
29.176
1.00
25.88
B


ATOM
3096
O
TYR
B
89
66.333
71.400
29.025
1.00
25.96
B


ATOM
3097
N
ARG
B
90
67.015
73.405
29.790
1.00
25.05
B


ATOM
3098
CA
ARG
B
90
65.732
73.778
30.386
1.00
21.94
B


ATOM
3099
CB
ARG
B
90
65.950
74.306
31.802
1.00
25.43
B


ATOM
3100
CG
ARG
B
90
66.836
73.368
32.650
1.00
35.30
B


ATOM
3101
CD
ARG
B
90
66.308
71.933
32.684
1.00
30.92
B


ATOM
3102
NE
ARG
B
90
65.162
71.857
33.578
1.00
38.77
B


ATOM
3103
CZ
ARG
B
90
64.577
70.721
33.950
1.00
43.50
B


ATOM
3104
NH1
ARG
B
90
65.038
69.562
33.500
1.00
42.19
B


ATOM
3105
NH2
ARG
B
90
63.529
70.750
34.771
1.00
39.16
B


ATOM
3106
C
ARG
B
90
65.001
74.823
29.580
1.00
26.84
B


ATOM
3107
O
ARG
B
90
65.540
75.372
28.606
1.00
21.63
B


ATOM
3108
N
HIS
B
91
63.767
75.095
29.999
1.00
24.57
B


ATOM
3109
CA
HIS
B
91
62.903
76.057
29.318
1.00
28.69
B


ATOM
3110
CB
HIS
B
91
61.570
76.143
30.062
1.00
26.54
B


ATOM
3111
CG
HIS
B
91
60.516
76.909
29.326
1.00
34.66
B


ATOM
3112
CD2
HIS
B
91
59.414
77.563
29.770
1.00
26.97
B


ATOM
3113
ND1
HIS
B
91
60.513
77.035
27.952
1.00
29.34
B


ATOM
3114
CE1
HIS
B
91
59.457
77.735
27.582
1.00
27.63
B


ATOM
3115
NE2
HIS
B
91
58.775
78.066
28.666
1.00
34.45
B


ATOM
3116
C
HIS
B
91
63.546
77.445
29.177
1.00
30.22
B


ATOM
3117
O
HIS
B
91
63.937
78.069
30.161
1.00
23.98
B


ATOM
3118
N
GLY
B
92
63.663
77.915
27.941
1.00
26.25
B


ATOM
3119
CA
GLY
B
92
64.260
79.209
27.702
1.00
20.48
B


ATOM
3120
C
GLY
B
92
65.752
79.118
27.458
1.00
24.40
B


ATOM
3121
O
GLY
B
92
66.310
80.102
27.019
1.00
24.34
B


ATOM
3122
N
ASP
B
93
66.407
77.974
27.724
1.00
20.35
B


ATOM
3123
CA
ASP
B
93
67.857
77.884
27.472
1.00
19.73
B


ATOM
3124
CB
ASP
B
93
68.487
76.575
27.992
1.00
23.61
B


ATOM
3125
CG
ASP
B
93
68.579
76.504
29.504
1.00
20.60
B


ATOM
3126
OD1
ASP
B
93
68.336
77.522
30.167
1.00
23.89
B


ATOM
3127
OD2
ASP
B
93
68.884
75.401
30.020
1.00
25.06
B


ATOM
3128
C
ASP
B
93
68.138
77.911
25.981
1.00
25.02
B


ATOM
3129
O
ASP
B
93
67.362
77.415
25.170
1.00
24.06
B


ATOM
3130
N
SER
B
94
69.298
78.422
25.625
1.00
21.77
B


ATOM
3131
CA
SER
B
94
69.641
78.511
24.229
1.00
27.05
B


ATOM
3132
CB
SER
B
94
69.779
79.972
23.834
1.00
28.57
B


ATOM
3133
OG
SER
B
94
70.417
80.035
22.587
1.00
38.84
B


ATOM
3134
C
SER
B
94
70.935
77.815
23.908
1.00
25.71
B


ATOM
3135
O
SER
B
94
71.833
77.727
24.753
1.00
26.52
B


ATOM
3136
N
VAL
B
95
71.051
77.331
22.678
1.00
27.36
B


ATOM
3137
CA
VAL
B
95
72.291
76.689
22.245
1.00
26.56
B


ATOM
3138
CB
VAL
B
95
72.096
75.178
22.088
1.00
26.82
B


ATOM
3139
CG1
VAL
B
95
73.282
74.581
21.344
1.00
34.06
B


ATOM
3140
CG2
VAL
B
95
71.908
74.549
23.460
1.00
30.69
B


ATOM
3141
C
VAL
B
95
72.729
77.273
20.908
1.00
30.07
B


ATOM
3142
O
VAL
B
95
71.903
77.496
20.019
1.00
26.03
B


ATOM
3143
N
THR
B
96
74.026
77.526
20.758
1.00
27.23
B


ATOM
3144
CA
THR
B
96
74.540
78.058
19.503
1.00
23.14
B


ATOM
3145
CB
THR
B
96
75.242
79.419
19.687
1.00
32.77
B


ATOM
3146
OG1
THR
B
96
74.292
80.383
20.163
1.00
26.94
B


ATOM
3147
CG2
THR
B
96
75.845
79.896
18.329
1.00
26.13
B


ATOM
3148
C
THR
B
96
75.550
77.071
18.915
1.00
33.62
B


ATOM
3149
O
THR
B
96
76.445
76.614
19.618
1.00
26.19
B


ATOM
3150
N
PHE
B
97
75.381
76.732
17.638
1.00
28.29
B


ATOM
3151
CA
PHE
B
97
76.249
75.788
16.922
1.00
27.82
B


ATOM
3152
CB
PHE
B
97
75.409
74.849
16.039
1.00
27.23
B


ATOM
3153
CG
PHE
B
97
74.598
73.858
16.796
1.00
30.99
B


ATOM
3154
CD1
PHE
B
97
73.325
74.183
17.257
1.00
28.10
B


ATOM
3155
CD2
PHE
B
97
75.149
72.633
17.153
1.00
24.15
B


ATOM
3156
CE1
PHE
B
97
72.608
73.310
18.081
1.00
29.54
B


ATOM
3157
CE2
PHE
B
97
74.434
71.756
17.975
1.00
26.96
B


ATOM
3158
CZ
PHE
B
97
73.155
72.101
18.444
1.00
28.76
B


ATOM
3159
C
PHE
B
97
77.247
76.479
15.980
1.00
33.87
B


ATOM
3160
O
PHE
B
97
77.088
77.663
15.628
1.00
30.90
B


ATOM
3161
N
ALA
B
98
78.274
75.725
15.582
1.00
33.98
B


ATOM
3162
CA
ALA
B
98
79.266
76.152
14.574
1.00
34.14
B


ATOM
3163
CB
ALA
B
98
80.551
76.651
15.211
1.00
38.55
B


ATOM
3164
C
ALA
B
98
79.544
74.880
13.785
1.00
36.15
B


ATOM
3165
O
ALA
B
98
79.370
73.775
14.312
1.00
35.98
B


ATOM
3166
N
CYS
B
99
79.953
75.018
12.527
1.00
38.50
B


ATOM
3167
CA
CYS
B
99
80.266
73.843
11.711
1.00
39.15
B


ATOM
3168
C
CYS
B
99
81.747
73.512
11.779
1.00
36.00
B


ATOM
3169
O
CYS
B
99
82.573
74.408
11.865
1.00
32.17
B


ATOM
3170
CB
CYS
B
99
79.901
74.086
10.251
1.00
42.12
B


ATOM
3171
SG
CYS
B
99
78.108
74.162
9.949
1.00
40.52
B


ATOM
3172
N
LYS
B
100
82.089
72.229
11.749
1.00
35.70
B


ATOM
3173
CA
LYS
B
100
83.498
71.842
11.770
1.00
40.35
B


ATOM
3174
CB
LYS
B
100
83.632
70.319
11.848
1.00
43.13
B


ATOM
3175
CG
LYS
B
100
83.185
69.707
13.148
1.00
38.59
B


ATOM
3176
CD
LYS
B
100
82.771
68.263
12.966
1.00
44.87
B


ATOM
3177
CE
LYS
B
100
82.689
67.562
14.314
1.00
48.06
B


ATOM
3178
NZ
LYS
B
100
82.353
66.122
14.196
1.00
53.59
B


ATOM
3179
C
LYS
B
100
84.190
72.329
10.482
1.00
45.56
B


ATOM
3180
O
LYS
B
100
83.532
72.770
9.525
1.00
42.57
B


ATOM
3181
N
THR
B
101
85.519
72.239
10.456
1.00
47.39
B


ATOM
3182
CA
THR
B
101
86.286
72.651
9.282
1.00
48.55
B


ATOM
3183
CB
THR
B
101
87.794
72.408
9.497
1.00
48.03
B


ATOM
3184
OG1
THR
B
101
88.281
73.285
10.521
1.00
47.14
B


ATOM
3185
CG2
THR
B
101
88.557
72.683
8.212
1.00
51.80
B


ATOM
3186
C
THR
B
101
85.816
71.876
8.042
1.00
47.72
B


ATOM
3187
O
THR
B
101
85.523
70.681
8.127
1.00
43.56
B


ATOM
3188
N
ASN
B
102
85.747
72.555
6.901
1.00
47.11
B


ATOM
3189
CA
ASN
B
102
85.294
71.943
5.647
1.00
48.36
B


ATOM
3190
CB
ASN
B
102
85.961
70.594
5.375
1.00
53.67
B


ATOM
3191
CG
ASN
B
102
87.461
70.703
5.219
1.00
58.65
B


ATOM
3192
OD1
ASN
B
102
87.980
71.763
4.856
1.00
59.24
B


ATOM
3193
ND2
ASN
B
102
88.171
69.599
5.484
1.00
61.19
B


ATOM
3194
C
ASN
B
102
83.797
71.728
5.632
1.00
49.36
B


ATOM
3195
O
ASN
B
102
83.284
70.965
4.814
1.00
51.85
B


ATOM
3196
N
PHE
B
103
83.097
72.378
6.555
1.00
48.85
B


ATOM
3197
CA
PHE
B
103
81.641
72.294
6.617
1.00
44.54
B


ATOM
3198
CB
PHE
B
103
81.179
71.539
7.855
1.00
44.01
B


ATOM
3199
CG
PHE
B
103
81.335
70.067
7.740
1.00
45.42
B


ATOM
3200
CD1
PHE
B
103
82.554
69.461
8.037
1.00
47.36
B


ATOM
3201
CD2
PHE
B
103
80.270
69.274
7.323
1.00
44.68
B


ATOM
3202
CE1
PHE
B
103
82.715
68.064
7.921
1.00
44.91
B


ATOM
3203
CE2
PHE
B
103
80.413
67.883
7.202
1.00
46.13
B


ATOM
3204
CZ
PHE
B
103
81.638
67.276
7.503
1.00
44.51
B


ATOM
3205
C
PHE
B
103
81.103
73.700
6.663
1.00
43.79
B


ATOM
3206
O
PHE
B
103
81.736
74.577
7.239
1.00
41.42
B


ATOM
3207
N
SER
B
104
79.939
73.908
6.052
1.00
46.45
B


ATOM
3208
CA
SER
B
104
79.306
75.216
6.007
1.00
47.47
B


ATOM
3209
CB
SER
B
104
79.176
75.706
4.570
1.00
53.61
B


ATOM
3210
OG
SER
B
104
77.886
75.379
4.057
1.00
61.85
B


ATOM
3211
C
SER
B
104
77.921
75.051
6.596
1.00
46.27
B


ATOM
3212
O
SER
B
104
77.285
74.007
6.425
1.00
46.36
B


ATOM
3213
N
MET
B
105
77.435
76.089
7.269
1.00
47.80
B


ATOM
3214
CA
MET
B
105
76.129
75.998
7.913
1.00
50.16
B


ATOM
3215
CB
MET
B
105
76.114
76.848
9.184
1.00
42.24
B


ATOM
3216
CG
MET
B
105
74.783
76.829
9.931
1.00
39.50
B


ATOM
3217
SD
MET
B
105
74.951
77.585
11.559
1.00
31.98
B


ATOM
3218
CE
MET
B
105
76.153
76.456
12.300
1.00
32.14
B


ATOM
3219
C
MET
B
105
74.948
76.398
7.046
1.00
52.75
B


ATOM
3220
O
MET
B
105
75.066
77.234
6.147
1.00
52.67
B


ATOM
3221
N
ASN
B
106
73.807
75.787
7.343
1.00
57.15
B


ATOM
3222
CA
ASN
B
106
72.560
76.072
6.664
1.00
56.25
B


ATOM
3223
CB
ASN
B
106
72.186
74.950
5.722
1.00
63.78
B


ATOM
3224
CG
ASN
B
106
72.678
75.210
4.332
1.00
72.25
B


ATOM
3225
OD1
ASN
B
106
72.787
76.372
3.916
1.00
78.63
B


ATOM
3226
ND2
ASN
B
106
72.995
74.141
3.597
1.00
74.20
B


ATOM
3227
C
ASN
B
106
71.504
76.200
7.727
1.00
54.17
B


ATOM
3228
O
ASN
B
106
71.303
75.277
8.508
1.00
56.24
B


ATOM
3229
N
GLY
B
107
70.833
77.342
7.762
1.00
50.67
B


ATOM
3230
CA
GLY
B
107
69.805
77.552
8.763
1.00
44.02
B


ATOM
3231
C
GLY
B
107
70.334
78.414
9.886
1.00
42.66
B


ATOM
3232
O
GLY
B
107
71.478
78.864
9.854
1.00
41.68
B


ATOM
3233
N
ASN
B
108
69.500
78.646
10.889
1.00
46.24
B


ATOM
3234
CA
ASN
B
108
69.878
79.464
12.032
1.00
47.08
B


ATOM
3235
CB
ASN
B
108
68.613
79.910
12.751
1.00
55.35
B


ATOM
3236
CG
ASN
B
108
67.793
80.851
11.903
1.00
63.30
B


ATOM
3237
OD1
ASN
B
108
68.164
82.013
11.722
1.00
67.44
B


ATOM
3238
ND2
ASN
B
108
66.691
80.352
11.346
1.00
68.11
B


ATOM
3239
C
ASN
B
108
70.808
78.723
12.981
1.00
39.04
B


ATOM
3240
O
ASN
B
108
70.560
77.577
13.347
1.00
33.47
B


ATOM
3241
N
LYS
B
109
71.858
79.406
13.402
1.00
34.53
B


ATOM
3242
CA
LYS
B
109
72.859
78.806
14.280
1.00
35.55
B


ATOM
3243
CB
LYS
B
109
74.138
79.632
14.217
1.00
35.53
B


ATOM
3244
CG
LYS
B
109
73.909
81.037
14.698
1.00
37.21
B


ATOM
3245
CD
LYS
B
109
75.176
81.691
15.171
1.00
43.67
B


ATOM
3246
CE
LYS
B
109
74.913
83.141
15.501
1.00
53.84
B


ATOM
3247
NZ
LYS
B
109
76.079
83.848
16.124
1.00
67.33
B


ATOM
3248
C
LYS
B
109
72.456
78.639
15.753
1.00
33.59
B


ATOM
3249
O
LYS
B
109
73.116
77.900
16.490
1.00
32.99
B


ATOM
3250
N
SER
B
110
71.409
79.337
16.198
1.00
29.02
B


ATOM
3251
CA
SER
B
110
70.972
79.239
17.597
1.00
31.62
B


ATOM
3252
CB
SER
B
110
71.068
80.588
18.298
1.00
28.39
B


ATOM
3253
OG
SER
B
110
72.395
81.073
18.276
1.00
35.28
B


ATOM
3254
C
SER
B
110
69.547
78.730
17.734
1.00
33.04
B


ATOM
3255
O
SER
B
110
68.665
79.049
16.935
1.00
28.18
B


ATOM
3256
N
VAL
B
111
69.336
77.927
18.761
1.00
32.94
B


ATOM
3257
CA
VAL
B
111
68.028
77.360
19.032
1.00
28.12
B


ATOM
3258
CB
VAL
B
111
67.998
75.911
18.507
1.00
34.53
B


ATOM
3259
CG1
VAL
B
111
69.117
75.074
19.143
1.00
27.59
B


ATOM
3260
CG2
VAL
B
111
66.694
75.304
18.785
1.00
41.47
B


ATOM
3261
C
VAL
B
111
67.729
77.488
20.539
1.00
26.65
B


ATOM
3262
O
VAL
B
111
68.631
77.621
21.359
1.00
27.71
B


ATOM
3263
N
TRP
B
112
66.447
77.496
20.866
1.00
26.33
B


ATOM
3264
CA
TRP
B
112
65.932
77.654
22.205
1.00
21.46
B


ATOM
3265
CB
TRP
B
112
65.023
78.895
22.287
1.00
27.43
B


ATOM
3266
CG
TRP
B
112
65.772
80.142
22.392
1.00
28.72
B


ATOM
3267
CD2
TRP
B
112
66.455
80.818
21.323
1.00
27.13
B


ATOM
3268
CE2
TRP
B
112
67.130
81.922
21.894
1.00
23.65
B


ATOM
3269
CE3
TRP
B
112
66.560
80.595
19.944
1.00
27.06
B


ATOM
3270
CD1
TRP
B
112
66.053
80.849
23.542
1.00
29.24
B


ATOM
3271
NE1
TRP
B
112
66.875
81.916
23.242
1.00
22.18
B


ATOM
3272
CZ2
TRP
B
112
67.896
82.804
21.132
1.00
24.55
B


ATOM
3273
CZ3
TRP
B
112
67.328
81.476
19.179
1.00
32.51
B


ATOM
3274
CH2
TRP
B
112
67.985
82.570
19.782
1.00
28.74
B


ATOM
3275
C
TRP
B
112
65.113
76.462
22.622
1.00
24.09
B


ATOM
3276
O
TRP
B
112
64.348
75.924
21.826
1.00
23.03
B


ATOM
3277
N
CYS
B
113
65.257
76.065
23.886
1.00
16.00
B


ATOM
3278
CA
CYS
B
113
64.507
74.942
24.421
1.00
22.66
B


ATOM
3279
C
CYS
B
113
63.090
75.442
24.775
1.00
27.37
B


ATOM
3280
O
CYS
B
113
62.892
76.248
25.702
1.00
23.71
B


ATOM
3281
CB
CYS
B
113
65.230
74.386
25.636
1.00
24.38
B


ATOM
3282
SG
CYS
B
113
64.295
73.084
26.474
1.00
25.70
B


ATOM
3283
N
GLN
B
114
62.105
74.973
24.014
1.00
22.77
B


ATOM
3284
CA
GLN
B
114
60.704
75.406
24.190
1.00
27.53
B


ATOM
3285
CB
GLN
B
114
59.993
75.312
22.852
1.00
23.87
B


ATOM
3286
CG
GLN
B
114
60.669
76.101
21.776
1.00
25.80
B


ATOM
3287
CD
GLN
B
114
59.934
75.957
20.455
1.00
34.33
B


ATOM
3288
OE1
GLN
B
114
59.540
74.850
20.084
1.00
40.82
B


ATOM
3289
NE2
GLN
B
114
59.749
77.062
19.744
1.00
28.51
B


ATOM
3290
C
GLN
B
114
59.918
74.632
25.242
1.00
24.17
B


ATOM
3291
O
GLN
B
114
60.324
73.557
25.638
1.00
24.70
B


ATOM
3292
N
ALA
B
115
58.806
75.187
25.704
1.00
28.17
B


ATOM
3293
CA
ALA
B
115
57.984
74.520
26.720
1.00
32.79
B


ATOM
3294
CB
ALA
B
115
56.769
75.392
27.068
1.00
30.00
B


ATOM
3295
C
ALA
B
115
57.523
73.107
26.316
1.00
34.03
B


ATOM
3296
O
ALA
B
115
57.338
72.237
27.170
1.00
33.21
B


ATOM
3297
N
ASN
B
116
57.343
72.881
25.020
1.00
36.70
B


ATOM
3298
CA
ASN
B
116
56.913
71.573
24.526
1.00
38.02
B


ATOM
3299
CB
ASN
B
116
56.230
71.728
23.168
1.00
34.90
B


ATOM
3300
CG
ASN
B
116
57.198
72.144
22.087
1.00
33.42
B


ATOM
3301
OD1
ASN
B
116
58.359
72.454
22.364
1.00
28.93
B


ATOM
3302
ND2
ASN
B
116
56.728
72.167
20.853
1.00
30.98
B


ATOM
3303
C
ASN
B
116
58.104
70.608
24.392
1.00
34.77
B


ATOM
3304
O
ASN
B
116
58.017
69.612
23.695
1.00
37.59
B


ATOM
3305
N
ASN
B
117
59.219
70.937
25.044
1.00
36.92
B


ATOM
3306
CA
ASN
B
117
60.425
70.094
25.072
1.00
31.01
B


ATOM
3307
CB
ASN
B
117
60.095
68.728
25.699
1.00
35.95
B


ATOM
3308
CG
ASN
B
117
59.327
68.855
27.022
1.00
41.69
B


ATOM
3309
OD1
ASN
B
117
58.165
68.448
27.116
1.00
48.82
B


ATOM
3310
ND2
ASN
B
117
59.966
69.428
28.041
1.00
38.48
B


ATOM
3311
C
ASN
B
117
61.155
69.913
23.753
1.00
35.04
B


ATOM
3312
O
ASN
B
117
61.989
69.010
23.584
1.00
30.97
B


ATOM
3313
N
MET
B
118
60.877
70.804
22.814
1.00
32.90
B


ATOM
3314
CA
MET
B
118
61.544
70.747
21.539
1.00
30.69
B


ATOM
3315
CB
MET
B
118
60.533
70.486
20.426
1.00
39.19
B


ATOM
3316
CG
MET
B
118
60.143
69.004
20.284
1.00
45.35
B


ATOM
3317
SD
MET
B
118
58.844
68.904
19.065
1.00
61.98
B


ATOM
3318
CE
MET
B
118
57.379
68.930
20.152
1.00
59.12
B


ATOM
3319
C
MET
B
118
62.299
72.033
21.290
1.00
32.74
B


ATOM
3320
O
MET
B
118
61.958
73.093
21.820
1.00
31.41
B


ATOM
3321
N
TRP
B
119
63.322
71.913
20.460
1.00
30.27
B


ATOM
3322
CA
TRP
B
119
64.197
73.007
20.122
1.00
32.04
B


ATOM
3323
CB
TRP
B
119
65.519
72.477
19.556
1.00
29.45
B


ATOM
3324
CG
TRP
B
119
66.377
71.888
20.618
1.00
34.08
B


ATOM
3325
CD2
TRP
B
119
67.091
72.613
21.638
1.00
29.55
B


ATOM
3326
CE2
TRP
B
119
67.641
71.653
22.514
1.00
32.16
B


ATOM
3327
CE3
TRP
B
119
67.309
73.974
21.891
1.00
27.61
B


ATOM
3328
CD1
TRP
B
119
66.533
70.563
20.907
1.00
27.66
B


ATOM
3329
NE1
TRP
B
119
67.287
70.419
22.045
1.00
29.97
B


ATOM
3330
CZ2
TRP
B
119
68.401
72.018
23.634
1.00
33.69
B


ATOM
3331
CZ3
TRP
B
119
68.067
74.333
23.002
1.00
28.67
B


ATOM
3332
CH2
TRP
B
119
68.603
73.356
23.860
1.00
29.17
B


ATOM
3333
C
TRP
B
119
63.672
74.059
19.188
1.00
35.37
B


ATOM
3334
O
TRP
B
119
62.946
73.750
18.264
1.00
34.01
B


ATOM
3335
N
GLY
B
120
64.129
75.279
19.507
1.00
44.82
B


ATOM
3336
CA
GLY
B
120
63.928
76.577
18.868
1.00
40.39
B


ATOM
3337
C
GLY
B
120
62.736
76.818
18.037
1.00
45.27
B


ATOM
3338
O
GLY
B
120
61.922
75.920
17.884
1.00
54.52
B


ATOM
3339
N
PRO
B
121
62.570
78.049
17.525
1.00
39.72
B


ATOM
3340
CD
PRO
B
121
63.331
79.268
17.805
1.00
34.64
B


ATOM
3341
CA
PRO
B
121
61.411
78.339
16.678
1.00
40.69
B


ATOM
3342
CB
PRO
B
121
61.355
79.869
16.674
1.00
33.54
B


ATOM
3343
CG
PRO
B
121
62.781
80.221
16.775
1.00
38.11
B


ATOM
3344
C
PRO
B
121
61.717
77.733
15.294
1.00
38.17
B


ATOM
3345
O
PRO
B
121
60.817
77.305
14.591
1.00
40.16
B


ATOM
3346
N
THR
B
122
62.994
77.693
14.930
1.00
33.84
B


ATOM
3347
CA
THR
B
122
63.424
77.113
13.660
1.00
38.91
B


ATOM
3348
CB
THR
B
122
64.548
77.939
12.988
1.00
42.26
B


ATOM
3349
OG1
THR
B
122
65.803
77.671
13.634
1.00
39.47
B


ATOM
3350
CG2
THR
B
122
64.259
79.431
13.106
1.00
40.58
B


ATOM
3351
C
THR
B
122
63.968
75.708
13.874
1.00
40.61
B


ATOM
3352
O
THR
B
122
64.163
75.266
14.999
1.00
43.52
B


ATOM
3353
N
ARG
B
123
64.220
75.005
12.784
1.00
42.70
B


ATOM
3354
CA
ARG
B
123
64.762
73.656
12.870
1.00
46.43
B


ATOM
3355
CB
ARG
B
123
64.505
72.912
11.549
1.00
48.19
B


ATOM
3356
CG
ARG
B
123
64.849
71.433
11.600
1.00
63.34
B


ATOM
3357
CD
ARG
B
123
64.109
70.638
10.517
1.00
71.72
B


ATOM
3358
NE
ARG
B
123
62.664
70.564
10.762
1.00
75.72
B


ATOM
3359
CZ
ARG
B
123
61.734
70.934
9.881
1.00
77.53
B


ATOM
3360
NH1
ARG
B
123
62.096
71.410
8.690
1.00
78.13
B


ATOM
3361
NH2
ARG
B
123
60.444
70.820
10.184
1.00
75.29
B


ATOM
3362
C
ARG
B
123
66.270
73.774
13.141
1.00
36.14
B


ATOM
3363
O
ARG
B
123
66.861
74.816
12.880
1.00
34.06
B


ATOM
3364
N
LEU
B
124
66.888
72.714
13.659
1.00
36.62
B


ATOM
3365
CA
LEU
B
124
68.326
72.743
13.939
1.00
35.68
B


ATOM
3366
CB
LEU
B
124
68.818
71.410
14.509
1.00
37.22
B


ATOM
3367
CG
LEU
B
124
68.399
71.019
15.933
1.00
38.68
B


ATOM
3368
CD1
LEU
B
124
69.008
69.670
16.342
1.00
37.12
B


ATOM
3369
CD2
LEU
B
124
68.860
72.097
16.874
1.00
34.15
B


ATOM
3370
C
LEU
B
124
69.081
73.029
12.656
1.00
37.07
B


ATOM
3371
O
LEU
B
124
68.701
72.584
11.583
1.00
35.87
B


ATOM
3372
N
PRO
B
125
70.168
73.787
12.750
1.00
40.36
B


ATOM
3373
CD
PRO
B
125
70.832
74.306
13.962
1.00
38.05
B


ATOM
3374
CA
PRO
B
125
70.933
74.085
11.538
1.00
35.47
B


ATOM
3375
CB
PRO
B
125
71.993
75.069
12.029
1.00
35.51
B


ATOM
3376
CG
PRO
B
125
72.224
74.641
13.460
1.00
35.57
B


ATOM
3377
C
PRO
B
125
71.535
72.794
11.002
1.00
40.45
B


ATOM
3378
O
PRO
B
125
71.432
71.739
11.640
1.00
33.92
B


ATOM
3379
N
THR
B
126
72.131
72.867
9.814
1.00
41.58
B


ATOM
3380
CA
THR
B
126
72.765
71.704
9.217
1.00
41.03
B


ATOM
3381
CB
THR
B
126
71.929
71.137
8.006
1.00
42.99
B


ATOM
3382
OG1
THR
B
126
71.625
72.182
7.075
1.00
45.10
B


ATOM
3383
CG2
THR
B
126
70.628
70.529
8.503
1.00
40.83
B


ATOM
3384
C
THR
B
126
74.157
72.116
8.777
1.00
41.16
B


ATOM
3385
O
THR
B
126
74.415
73.299
8.544
1.00
40.35
B


ATOM
3386
N
CYS
B
127
75.074
71.158
8.716
1.00
42.86
B


ATOM
3387
CA
CYS
B
127
76.433
71.468
8.279
1.00
48.44
B


ATOM
3388
C
CYS
B
127
76.774
70.561
7.098
1.00
53.76
B


ATOM
3389
O
CYS
B
127
76.711
69.336
7.219
1.00
55.59
B


ATOM
3390
CB
CYS
B
127
77.440
71.249
9.422
1.00
49.29
B


ATOM
3391
SG
CYS
B
127
77.365
72.471
10.779
1.00
41.21
B


ATOM
3392
N
VAL
B
128
77.117
71.159
5.960
1.00
52.99
B


ATOM
3393
CA
VAL
B
128
77.466
70.386
4.764
1.00
58.11
B


ATOM
3394
CB
VAL
B
128
76.479
70.674
3.612
1.00
60.17
B


ATOM
3395
CG1
VAL
B
128
76.356
72.179
3.395
1.00
59.61
B


ATOM
3396
CG2
VAL
B
128
76.974
70.005
2.322
1.00
62.78
B


ATOM
3397
C
VAL
B
128
78.881
70.738
4.290
1.00
55.96
B


ATOM
3398
O
VAL
B
128
79.307
71.875
4.427
1.00
56.76
B


ATOM
3399
N
SER
B
129
79.604
69.763
3.747
1.00
58.88
B


ATOM
3400
CA
SER
B
129
80.976
69.974
3.241
1.00
63.04
B


ATOM
3401
CB
SER
B
129
81.489
68.685
2.616
1.00
64.42
B


ATOM
3402
OG
SER
B
129
80.566
68.249
1.633
1.00
74.77
B


ATOM
3403
C
SER
B
129
81.061
71.092
2.194
1.00
60.01
B


ATOM
3404
O
SER
B
129
82.054
71.856
2.166
1.00
59.63
B


ATOM
3405
OXT
SER
B
129
80.121
71.165
1.381
1.00
61.43
B


END
















TABLE 3





lists the atomic coordinates for a crystal of SCR1-2 of human CR2 (SEQ ID NO: 4).


























ATOM
2419
CB
ALA
B
1
64.181
107.666
4.123
1.00
39.20
B


ATOM
2420
C
ALA
B
1
62.836
105.816
3.113
1.00
41.03
B


ATOM
2421
O
ALA
B
1
61.659
105.713
3.477
1.00
42.08
B


ATOM
2422
N
ALA
B
1
62.413
108.231
2.458
1.00
40.18
B


ATOM
2423
CA
ALA
B
1
63.447
107.201
2.861
1.00
43.96
B


ATOM
2424
N
ILE
B
2
63.636
104.759
2.923
1.00
39.63
B


ATOM
2425
CA
ILE
B
2
63.175
103.383
3.141
1.00
37.01
B


ATOM
2426
CB
ILE
B
2
64.245
102.349
2.700
1.00
32.57
B


ATOM
2427
CG2
ILE
B
2
63.775
100.936
3.064
1.00
35.80
B


ATOM
2428
CG1
ILE
B
2
64.457
102.422
1.178
1.00
34.01
B


ATOM
2429
CD1
ILE
B
2
65.479
101.372
0.650
1.00
31.21
B


ATOM
2430
C
ILE
B
2
62.831
103.151
4.633
1.00
38.27
B


ATOM
2431
O
ILE
B
2
63.565
103.577
5.528
1.00
32.71
B


ATOM
2432
N
SER
B
3
61.714
102.486
4.897
1.00
30.65
B


ATOM
2433
CA
SER
B
3
61.316
102.226
6.272
1.00
26.19
B


ATOM
2434
CB
SER
B
3
60.245
103.236
6.689
1.00
31.79
B


ATOM
2435
OG
SER
B
3
59.060
103.020
5.926
1.00
27.35
B


ATOM
2436
C
SER
B
3
60.744
100.826
6.435
1.00
30.38
B


ATOM
2437
O
SER
B
3
60.495
100.132
5.446
1.00
28.89
B


ATOM
2438
N
CYS
B
4
60.559
100.410
7.690
1.00
27.89
B


ATOM
2439
CA
CYS
B
4
59.941
99.127
8.003
1.00
27.29
B


ATOM
2440
C
CYS
B
4
58.609
99.528
8.600
1.00
26.13
B


ATOM
2441
O
CYS
B
4
58.446
100.666
9.061
1.00
29.64
B


ATOM
2442
CB
CYS
B
4
60.731
98.328
9.056
1.00
27.46
B


ATOM
2443
SG
CYS
B
4
62.214
97.531
8.369
1.00
27.76
B


ATOM
2444
N
GLY
B
5
57.648
98.622
8.561
1.00
22.65
B


ATOM
2445
CA
GLY
B
5
56.366
98.916
9.155
1.00
23.57
B


ATOM
2446
C
GLY
B
5
56.452
98.570
10.643
1.00
24.00
B


ATOM
2447
O
GLY
B
5
57.504
98.199
11.181
1.00
23.18
B


ATOM
2448
N
SER
B
6
55.322
98.698
11.313
1.00
21.65
B


ATOM
2449
CA
SER
B
6
55.204
98.441
12.733
1.00
22.23
B


ATOM
2450
CB
SER
B
6
53.722
98.511
13.098
1.00
27.49
B


ATOM
2451
OG
SER
B
6
53.543
98.605
14.483
1.00
33.82
B


ATOM
2452
C
SER
B
6
55.785
97.062
13.091
1.00
26.45
B


ATOM
2453
O
SER
B
6
55.489
96.081
12.439
1.00
22.14
B


ATOM
2454
N
PRO
B
7
56.639
96.980
14.122
1.00
26.74
B


ATOM
2455
CD
PRO
B
7
57.230
98.026
14.973
1.00
25.89
B


ATOM
2456
CA
PRO
B
7
57.191
95.661
14.468
1.00
25.01
B


ATOM
2457
CB
PRO
B
7
58.175
95.984
15.592
1.00
22.52
B


ATOM
2458
CG
PRO
B
7
57.589
97.228
16.232
1.00
26.49
B


ATOM
2459
C
PRO
B
7
56.133
94.627
14.894
1.00
27.04
B


ATOM
2460
O
PRO
B
7
55.061
94.961
15.414
1.00
24.19
B


ATOM
2461
N
PRO
B
8
56.439
93.345
14.700
1.00
24.37
B


ATOM
2462
CD
PRO
B
8
57.540
92.754
13.936
1.00
26.75
B


ATOM
2463
CA
PRO
B
8
55.431
92.366
15.099
1.00
25.28
B


ATOM
2464
CB
PRO
B
8
55.974
91.048
14.538
1.00
31.15
B


ATOM
2465
CG
PRO
B
8
57.401
91.314
14.245
1.00
33.33
B


ATOM
2466
C
PRO
B
8
55.228
92.382
16.613
1.00
30.70
B


ATOM
2467
O
PRO
B
8
56.180
92.527
17.364
1.00
31.22
B


ATOM
2468
N
PRO
B
9
53.971
92.314
17.074
1.00
30.39
B


ATOM
2469
CD
PRO
B
9
52.724
92.280
16.275
1.00
37.82
B


ATOM
2470
CA
PRO
B
9
53.686
92.322
18.518
1.00
32.22
B


ATOM
2471
CB
PRO
B
9
52.155
92.415
18.589
1.00
31.66
B


ATOM
2472
CG
PRO
B
9
51.705
91.731
17.265
1.00
31.37
B


ATOM
2473
C
PRO
B
9
54.190
91.062
19.209
1.00
26.54
B


ATOM
2474
O
PRO
B
9
54.542
90.072
18.563
1.00
23.13
B


ATOM
2475
N
ILE
B
10
54.214
91.111
20.526
1.00
25.96
B


ATOM
2476
CA
ILE
B
10
54.651
89.969
21.302
1.00
34.36
B


ATOM
2477
CB
ILE
B
10
56.150
90.068
21.770
1.00
24.92
B


ATOM
2478
CG2
ILE
B
10
56.398
91.363
22.562
1.00
24.04
B


ATOM
2479
CG1
ILE
B
10
56.491
88.795
22.581
1.00
30.43
B


ATOM
2480
CD1
ILE
B
10
57.991
88.536
22.813
1.00
24.83
B


ATOM
2481
C
ILE
B
10
53.753
89.810
22.511
1.00
33.31
B


ATOM
2482
O
ILE
B
10
53.743
90.647
23.412
1.00
32.83
B


ATOM
2483
N
LEU
B
11
52.992
88.724
22.525
1.00
30.71
B


ATOM
2484
CA
LEU
B
11
52.108
88.483
23.659
1.00
34.63
B


ATOM
2485
CB
LEU
B
11
51.217
87.277
23.365
1.00
36.50
B


ATOM
2486
CG
LEU
B
11
50.279
87.435
22.161
1.00
41.37
B


ATOM
2487
CD1
LEU
B
11
49.708
86.073
21.769
1.00
42.99
B


ATOM
2488
CD2
LEU
B
11
49.142
88.425
22.495
1.00
39.65
B


ATOM
2489
C
LEU
B
11
52.912
88.247
24.946
1.00
33.29
B


ATOM
2490
O
LEU
B
11
53.905
87.517
24.948
1.00
28.06
B


ATOM
2491
N
ASN
B
12
52.463
88.872
26.032
1.00
31.32
B


ATOM
2492
CA
ASN
B
12
53.086
88.753
27.328
1.00
30.29
B


ATOM
2493
CB
ASN
B
12
53.025
87.303
27.802
1.00
32.27
B


ATOM
2494
CG
ASN
B
12
51.612
86.875
28.083
1.00
38.86
B


ATOM
2495
OD1
ASN
B
12
50.917
87.526
28.864
1.00
38.55
B


ATOM
2496
ND2
ASN
B
12
51.161
85.805
27.431
1.00
34.66
B


ATOM
2497
C
ASN
B
12
54.510
89.250
27.371
1.00
32.36
B


ATOM
2498
O
ASN
B
12
55.277
88.858
28.239
1.00
32.55
B


ATOM
2499
N
GLY
B
13
54.849
90.130
26.439
1.00
28.46
B


ATOM
2500
CA
GLY
B
13
56.187
90.671
26.400
1.00
28.72
B


ATOM
2501
C
GLY
B
13
56.147
92.166
26.169
1.00
29.82
B


ATOM
2502
O
GLY
B
13
55.075
92.780
26.153
1.00
34.03
B


ATOM
2503
N
ARG
B
14
57.324
92.742
25.983
1.00
28.15
B


ATOM
2504
CA
ARG
B
14
57.486
94.166
25.739
1.00
31.15
B


ATOM
2505
CB
ARG
B
14
58.198
94.882
26.897
1.00
34.62
B


ATOM
2506
CG
ARG
B
14
57.388
95.196
28.116
1.00
41.44
B


ATOM
2507
CD
ARG
B
14
58.240
95.962
29.112
1.00
42.08
B


ATOM
2508
NE
ARG
B
14
57.526
96.080
30.373
1.00
49.55
B


ATOM
2509
CZ
ARG
B
14
58.023
96.595
31.487
1.00
48.40
B


ATOM
2510
NH1
ARG
B
14
59.261
97.065
31.509
1.00
40.73
B


ATOM
2511
NH2
ARG
B
14
57.276
96.602
32.593
1.00
56.30
B


ATOM
2512
C
ARG
B
14
58.409
94.342
24.567
1.00
23.56
B


ATOM
2513
O
ARG
B
14
59.232
93.480
24.280
1.00
23.51
B


ATOM
2514
N
ILE
B
15
58.319
95.516
23.955
1.00
27.48
B


ATOM
2515
CA
ILE
B
15
59.171
95.893
22.841
1.00
26.75
B


ATOM
2516
CB
ILE
B
15
58.345
96.176
21.580
1.00
26.32
B


ATOM
2517
CG2
ILE
B
15
59.250
96.815
20.497
1.00
29.30
B


ATOM
2518
CG1
ILE
B
15
57.656
94.902
21.122
1.00
30.62
B


ATOM
2519
CD1
ILE
B
15
56.760
95.100
19.889
1.00
35.23
B


ATOM
2520
C
ILE
B
15
59.767
97.212
23.279
1.00
26.59
B


ATOM
2521
O
ILE
B
15
59.050
98.050
23.818
1.00
30.94
B


ATOM
2522
N
SER
B
16
61.055
97.409
23.036
1.00
23.15
B


ATOM
2523
CA
SER
B
16
61.709
98.654
23.393
1.00
32.61
B


ATOM
2524
CB
SER
B
16
63.187
98.567
23.057
1.00
29.32
B


ATOM
2525
OG
SER
B
16
63.359
98.151
21.717
1.00
35.31
B


ATOM
2526
C
SER
B
16
61.082
99.839
22.639
1.00
41.27
B


ATOM
2527
O
SER
B
16
60.466
99.674
21.576
1.00
36.78
B


ATOM
2528
N
TYR
B
17
61.260
101.035
23.193
1.00
42.89
B


ATOM
2529
CA
TYR
B
17
60.719
102.267
22.624
1.00
46.29
B


ATOM
2530
CB
TYR
B
17
60.932
103.436
23.623
1.00
44.99
B


ATOM
2531
CG
TYR
B
17
60.026
103.380
24.865
1.00
47.02
B


ATOM
2532
CD1
TYR
B
17
60.510
103.694
26.151
1.00
49.03
B


ATOM
2533
CE1
TYR
B
17
59.664
103.655
27.283
1.00
48.52
B


ATOM
2534
CD2
TYR
B
17
58.686
103.025
24.748
1.00
51.67
B


ATOM
2535
CE2
TYR
B
17
57.833
102.974
25.861
1.00
53.32
B


ATOM
2536
CZ
TYR
B
17
58.319
103.292
27.122
1.00
56.82
B


ATOM
2537
OH
TYR
B
17
57.430
103.259
28.190
1.00
55.53
B


ATOM
2538
C
TYR
B
17
61.397
102.551
21.285
1.00
46.15
B


ATOM
2539
O
TYR
B
17
62.600
102.375
21.146
1.00
50.00
B


ATOM
2540
N
TYR
B
18
60.623
102.952
20.281
1.00
46.05
B


ATOM
2541
CA
TYR
B
18
61.204
103.270
18.975
1.00
44.93
B


ATOM
2542
CB
TYR
B
18
61.076
102.076
18.010
1.00
37.78
B


ATOM
2543
CG
TYR
B
18
59.645
101.669
17.711
1.00
37.10
B


ATOM
2544
CD1
TYR
B
18
58.890
102.343
16.750
1.00
31.24
B


ATOM
2545
CE1
TYR
B
18
57.573
101.980
16.501
1.00
34.43
B


ATOM
2546
CD2
TYR
B
18
59.040
100.622
18.415
1.00
34.57
B


ATOM
2547
CE2
TYR
B
18
57.730
100.255
18.184
1.00
36.06
B


ATOM
2548
CZ
TYR
B
18
57.000
100.939
17.218
1.00
35.02
B


ATOM
2549
OH
TYR
B
18
55.706
100.565
16.967
1.00
33.22
B


ATOM
2550
C
TYR
B
18
60.524
104.514
18.375
1.00
40.62
B


ATOM
2551
O
TYR
B
18
59.356
104.784
18.632
1.00
40.57
B


ATOM
2552
N
SER
B
19
61.280
105.260
17.586
1.00
42.39
B


ATOM
2553
CA
SER
B
19
60.786
106.464
16.931
1.00
49.17
B


ATOM
2554
CB
SER
B
19
61.922
107.472
16.789
1.00
49.59
B


ATOM
2555
OG
SER
B
19
63.070
106.843
16.237
1.00
54.17
B


ATOM
2556
C
SER
B
19
60.213
106.155
15.540
1.00
51.20
B


ATOM
2557
O
SER
B
19
60.569
105.165
14.900
1.00
42.62
B


ATOM
2558
N
THR
B
20
59.347
107.041
15.073
1.00
48.91
B


ATOM
2559
CA
THR
B
20
58.706
106.898
13.783
1.00
49.40
B


ATOM
2560
CB
THR
B
20
57.166
107.002
13.991
1.00
52.73
B


ATOM
2561
OG1
THR
B
20
56.483
106.279
12.968
1.00
60.25
B


ATOM
2562
CG2
THR
B
20
56.714
108.456
13.943
1.00
51.71
B


ATOM
2563
C
THR
B
20
59.222
108.022
12.844
1.00
47.48
B


ATOM
2564
O
THR
B
20
59.460
109.147
13.280
1.00
50.56
B


ATOM
2565
N
PRO
B
21
59.427
107.720
11.551
1.00
46.52
B


ATOM
2566
CD
PRO
B
21
59.801
108.745
10.551
1.00
44.33
B


ATOM
2567
CA
PRO
B
21
59.204
106.417
10.909
1.00
43.20
B


ATOM
2568
CB
PRO
B
21
59.137
106.769
9.422
1.00
43.49
B


ATOM
2569
CG
PRO
B
21
60.103
107.918
9.305
1.00
44.19
B


ATOM
2570
C
PRO
B
21
60.342
105.456
11.248
1.00
37.71
B


ATOM
2571
O
PRO
B
21
61.393
105.891
11.674
1.00
38.42
B


ATOM
2572
N
THR
B
22
60.123
104.155
11.067
1.00
40.00
B


ATOM
2573
CA
THR
B
22
61.139
103.150
11.382
1.00
37.29
B


ATOM
2574
CB
THR
B
22
60.521
101.715
11.578
1.00
33.63
B


ATOM
2575
OG1
THR
B
22
59.497
101.719
12.600
1.00
36.55
B


ATOM
2576
CG2
THR
B
22
61.626
100.734
11.963
1.00
26.59
B


ATOM
2577
C
THR
B
22
62.143
103.079
10.221
1.00
35.94
B


ATOM
2578
O
THR
B
22
61.851
102.484
9.183
1.00
33.29
B


ATOM
2579
N
ALA
B
23
63.326
103.663
10.410
1.00
33.77
B


ATOM
2580
CA
ALA
B
23
64.367
103.677
9.380
1.00
30.19
B


ATOM
2581
CB
ALA
B
23
65.314
104.852
9.615
1.00
28.90
B


ATOM
2582
C
ALA
B
23
65.192
102.399
9.300
1.00
30.98
B


ATOM
2583
O
ALA
B
23
65.331
101.693
10.284
1.00
23.97
B


ATOM
2584
N
VAL
B
24
65.736
102.127
8.112
1.00
27.61
B


ATOM
2585
CA
VAL
B
24
66.591
100.982
7.874
1.00
26.92
B


ATOM
2586
CB
VAL
B
24
67.197
101.042
6.460
1.00
29.41
B


ATOM
2587
CG1
VAL
B
24
68.357
100.043
6.326
1.00
28.58
B


ATOM
2588
CG2
VAL
B
24
66.107
100.729
5.430
1.00
26.92
B


ATOM
2589
C
VAL
B
24
67.707
101.091
8.898
1.00
32.79
B


ATOM
2590
O
VAL
B
24
68.266
102.166
9.077
1.00
26.10
B


ATOM
2591
N
GLY
B
25
68.026
100.000
9.586
1.00
28.01
B


ATOM
2592
CA
GLY
B
25
69.070
100.081
10.596
1.00
31.93
B


ATOM
2593
C
GLY
B
25
68.516
100.210
12.007
1.00
34.29
B


ATOM
2594
O
GLY
B
25
69.251
100.080
12.966
1.00
28.44
B


ATOM
2595
N
THR
B
26
67.227
100.486
12.148
1.00
26.99
B


ATOM
2596
CA
THR
B
26
66.650
100.568
13.474
1.00
29.37
B


ATOM
2597
CB
THR
B
26
65.179
100.994
13.403
1.00
30.78
B


ATOM
2598
OG1
THR
B
26
65.111
102.295
12.808
1.00
26.87
B


ATOM
2599
CG2
THR
B
26
64.524
101.000
14.833
1.00
23.56
B


ATOM
2600
C
THR
B
26
66.713
99.183
14.136
1.00
32.07
B


ATOM
2601
O
THR
B
26
66.462
98.171
13.473
1.00
28.44
B


ATOM
2602
N
VAL
B
27
67.056
99.143
15.429
1.00
28.90
B


ATOM
2603
CA
VAL
B
27
67.124
97.891
16.200
1.00
29.71
B


ATOM
2604
CB
VAL
B
27
68.507
97.703
16.858
1.00
36.67
B


ATOM
2605
CG1
VAL
B
27
68.477
96.507
17.808
1.00
31.87
B


ATOM
2606
CG2
VAL
B
27
69.554
97.478
15.793
1.00
32.00
B


ATOM
2607
C
VAL
B
27
66.054
97.946
17.292
1.00
26.09
B


ATOM
2608
O
VAL
B
27
65.961
98.921
18.029
1.00
28.74
B


ATOM
2609
N
ILE
B
28
65.230
96.913
17.349
1.00
28.74
B


ATOM
2610
CA
ILE
B
28
64.126
96.780
18.294
1.00
34.25
B


ATOM
2611
CB
ILE
B
28
62.802
96.400
17.552
1.00
38.10
B


ATOM
2612
CG2
ILE
B
28
61.752
95.929
18.516
1.00
49.78
B


ATOM
2613
CG1
ILE
B
28
62.260
97.598
16.808
1.00
46.71
B


ATOM
2614
CD1
ILE
B
28
62.252
98.826
17.612
1.00
40.93
B


ATOM
2615
C
ILE
B
28
64.491
95.619
19.223
1.00
38.36
B


ATOM
2616
O
ILE
B
28
65.063
94.626
18.769
1.00
29.90
B


ATOM
2617
N
ARG
B
29
64.134
95.724
20.503
1.00
30.42
B


ATOM
2618
CA
ARG
B
29
64.470
94.673
21.458
1.00
32.43
B


ATOM
2619
CB
ARG
B
29
65.468
95.242
22.461
1.00
38.54
B


ATOM
2620
CG
ARG
B
29
66.234
94.231
23.273
1.00
56.74
B


ATOM
2621
CD
ARG
B
29
67.479
94.939
23.821
1.00
68.43
B


ATOM
2622
NE
ARG
B
29
68.210
95.573
22.722
1.00
77.31
B


ATOM
2623
CZ
ARG
B
29
69.134
94.960
21.983
1.00
81.82
B


ATOM
2624
NH1
ARG
B
29
69.454
93.693
22.237
1.00
82.00
B


ATOM
2625
NH2
ARG
B
29
69.723
95.603
20.976
1.00
82.81
B


ATOM
2626
C
ARG
B
29
63.222
94.132
22.168
1.00
26.06
B


ATOM
2627
O
ARG
B
29
62.432
94.884
22.704
1.00
20.11
B


ATOM
2628
N
TYR
B
30
63.032
92.823
22.133
1.00
22.95
B


ATOM
2629
CA
TYR
B
30
61.886
92.206
22.787
1.00
31.30
B


ATOM
2630
CB
TYR
B
30
61.299
91.096
21.905
1.00
28.61
B


ATOM
2631
CG
TYR
B
30
60.647
91.547
20.605
1.00
29.37
B


ATOM
2632
CD1
TYR
B
30
61.389
92.135
19.579
1.00
24.77
B


ATOM
2633
CE1
TYR
B
30
60.781
92.553
18.387
1.00
19.11
B


ATOM
2634
CD2
TYR
B
30
59.282
91.378
20.403
1.00
29.03
B


ATOM
2635
CE2
TYR
B
30
58.674
91.785
19.215
1.00
26.35
B


ATOM
2636
CZ
TYR
B
30
59.429
92.379
18.216
1.00
23.41
B


ATOM
2637
OH
TYR
B
30
58.793
92.836
17.083
1.00
26.05
B


ATOM
2638
C
TYR
B
30
62.340
91.579
24.126
1.00
28.93
B


ATOM
2639
O
TYR
B
30
63.497
91.168
24.283
1.00
24.49
B


ATOM
2640
N
SER
B
31
61.418
91.488
25.067
1.00
26.50
B


ATOM
2641
CA
SER
B
31
61.697
90.903
26.369
1.00
24.95
B


ATOM
2642
CB
SER
B
31
62.304
91.946
27.308
1.00
21.32
B


ATOM
2643
OG
SER
B
31
61.426
93.028
27.483
1.00
26.55
B


ATOM
2644
C
SER
B
31
60.388
90.378
26.954
1.00
29.25
B


ATOM
2645
O
SER
B
31
59.287
90.780
26.529
1.00
22.45
B


ATOM
2646
N
CYS
B
32
60.515
89.484
27.930
1.00
27.99
B


ATOM
2647
CA
CYS
B
32
59.364
88.868
28.606
1.00
32.41
B


ATOM
2648
C
CYS
B
32
59.371
89.122
30.121
1.00
38.37
B


ATOM
2649
O
CYS
B
32
60.431
89.311
30.719
1.00
37.82
B


ATOM
2650
CB
CYS
B
32
59.371
87.347
28.400
1.00
30.70
B


ATOM
2651
SG
CYS
B
32
59.450
86.770
26.677
1.00
32.11
B


ATOM
2652
N
SER
B
33
58.178
89.101
30.717
1.00
51.37
B


ATOM
2653
CA
SER
B
33
57.995
89.265
32.163
1.00
56.15
B


ATOM
2654
CB
SER
B
33
56.544
89.013
32.552
1.00
60.18
B


ATOM
2655
OG
SER
B
33
55.675
89.714
31.672
1.00
66.23
B


ATOM
2656
C
SER
B
33
58.859
88.260
32.894
1.00
57.27
B


ATOM
2657
O
SER
B
33
59.535
87.427
32.274
1.00
60.30
B


ATOM
2658
N
GLY
B
34
58.805
88.290
34.219
1.00
53.29
B


ATOM
2659
CA
GLY
B
34
59.662
87.391
34.976
1.00
47.70
B


ATOM
2660
C
GLY
B
34
59.278
85.922
34.987
1.00
44.48
B


ATOM
2661
O
GLY
B
34
60.129
85.033
35.135
1.00
38.34
B


ATOM
2662
N
THR
B
35
57.993
85.660
34.818
1.00
40.51
B


ATOM
2663
CA
THR
B
35
57.524
84.290
34.860
1.00
40.76
B


ATOM
2664
CB
THR
B
35
56.225
84.239
35.644
1.00
41.73
B


ATOM
2665
OG1
THR
B
35
55.258
85.077
35.014
1.00
48.51
B


ATOM
2666
CG2
THR
B
35
56.468
84.761
37.057
1.00
44.89
B


ATOM
2667
C
THR
B
35
57.369
83.691
33.470
1.00
39.51
B


ATOM
2668
O
THR
B
35
56.786
82.613
33.286
1.00
31.97
B


ATOM
2669
N
PHE
B
36
57.924
84.399
32.491
1.00
35.56
B


ATOM
2670
CA
PHE
B
36
57.873
83.955
31.112
1.00
31.01
B


ATOM
2671
CB
PHE
B
36
57.146
84.967
30.250
1.00
33.05
B


ATOM
2672
CG
PHE
B
36
55.673
84.918
30.396
1.00
35.23
B


ATOM
2673
CD1
PHE
B
36
55.040
85.573
31.444
1.00
35.53
B


ATOM
2674
CD2
PHE
B
36
54.909
84.204
29.483
1.00
31.53
B


ATOM
2675
CE1
PHE
B
36
53.663
85.497
31.570
1.00
37.58
B


ATOM
2676
CE2
PHE
B
36
53.549
84.126
29.604
1.00
32.60
B


ATOM
2677
CZ
PHE
B
36
52.918
84.769
30.638
1.00
32.38
B


ATOM
2678
C
PHE
B
36
59.276
83.798
30.577
1.00
32.83
B


ATOM
2679
O
PHE
B
36
60.212
84.347
31.125
1.00
33.40
B


ATOM
2680
N
ARG
B
37
59.427
83.049
29.498
1.00
30.99
B


ATOM
2681
CA
ARG
B
37
60.741
82.896
28.916
1.00
29.69
B


ATOM
2682
CB
ARG
B
37
61.201
81.458
29.083
1.00
30.62
B


ATOM
2683
CG
ARG
B
37
61.442
81.072
30.541
1.00
35.61
B


ATOM
2684
CD
ARG
B
37
62.492
81.992
31.177
1.00
39.39
B


ATOM
2685
NE
ARG
B
37
62.303
82.044
32.629
1.00
49.28
B


ATOM
2686
CZ
ARG
B
37
62.847
81.168
33.451
1.00
47.41
B


ATOM
2687
NH1
ARG
B
37
63.614
80.212
32.947
1.00
53.00
B


ATOM
2688
NH2
ARG
B
37
62.602
81.223
34.751
1.00
44.10
B


ATOM
2689
C
ARG
B
37
60.713
83.280
27.439
1.00
32.05
B


ATOM
2690
O
ARG
B
37
59.809
82.874
26.700
1.00
25.63
B


ATOM
2691
N
LEU
B
38
61.706
84.050
27.011
1.00
23.96
B


ATOM
2692
CA
LEU
B
38
61.802
84.470
25.621
1.00
30.62
B


ATOM
2693
CB
LEU
B
38
62.655
85.738
25.508
1.00
23.25
B


ATOM
2694
CG
LEU
B
38
62.739
86.365
24.105
1.00
28.16
B


ATOM
2695
CD1
LEU
B
38
61.376
86.948
23.659
1.00
20.01
B


ATOM
2696
CD2
LEU
B
38
63.753
87.486
24.132
1.00
32.19
B


ATOM
2697
C
LEU
B
38
62.386
83.361
24.745
1.00
27.63
B


ATOM
2698
O
LEU
B
38
63.447
82.793
25.036
1.00
27.98
B


ATOM
2699
N
ILE
B
39
61.651
83.017
23.691
1.00
30.18
B


ATOM
2700
CA
ILE
B
39
62.073
81.983
22.743
1.00
27.37
B


ATOM
2701
CB
ILE
B
39
60.940
80.959
22.492
1.00
30.75
B


ATOM
2702
CG2
ILE
B
39
61.438
79.878
21.538
1.00
29.27
B


ATOM
2703
CG1
ILE
B
39
60.455
80.359
23.822
1.00
30.90
B


ATOM
2704
CD1
ILE
B
39
61.491
79.535
24.559
1.00
26.44
B


ATOM
2705
C
ILE
B
39
62.403
82.668
21.418
1.00
29.53
B


ATOM
2706
O
ILE
B
39
61.510
83.256
20.797
1.00
24.87
B


ATOM
2707
N
GLY
B
40
63.676
82.609
20.998
1.00
21.97
B


ATOM
2708
CA
GLY
B
40
64.084
83.239
19.751
1.00
23.62
B


ATOM
2709
C
GLY
B
40
64.957
84.450
19.975
1.00
22.85
B


ATOM
2710
O
GLY
B
40
65.065
84.921
21.104
1.00
21.42
B


ATOM
2711
N
GLU
B
41
65.592
84.958
18.925
1.00
27.33
B


ATOM
2712
CA
GLU
B
41
66.472
86.133
19.065
1.00
29.50
B


ATOM
2713
CB
GLU
B
41
67.138
86.471
17.730
1.00
33.78
B


ATOM
2714
CG
GLU
B
41
68.634
86.193
17.721
1.00
52.17
B


ATOM
2715
CD
GLU
B
41
69.411
87.123
18.648
1.00
57.74
B


ATOM
2716
OE1
GLU
B
41
70.414
86.641
19.231
1.00
66.30
B


ATOM
2717
OE2
GLU
B
41
69.034
88.326
18.791
1.00
53.76
B


ATOM
2718
C
GLU
B
41
65.667
87.333
19.541
1.00
30.40
B


ATOM
2719
O
GLU
B
41
64.601
87.619
18.995
1.00
27.22
B


ATOM
2720
N
LYS
B
42
66.197
88.049
20.528
1.00
27.86
B


ATOM
2721
CA
LYS
B
42
65.538
89.215
21.135
1.00
32.03
B


ATOM
2722
CB
LYS
B
42
66.171
89.495
22.504
1.00
37.19
B


ATOM
2723
CG
LYS
B
42
67.610
89.990
22.379
1.00
43.78
B


ATOM
2724
CD
LYS
B
42
68.196
90.536
23.674
1.00
54.68
B


ATOM
2725
CE
LYS
B
42
68.055
89.538
24.812
1.00
62.51
B


ATOM
2726
NZ
LYS
B
42
68.438
88.139
24.443
1.00
69.20
B


ATOM
2727
C
LYS
B
42
65.586
90.530
20.331
1.00
32.32
B


ATOM
2728
O
LYS
B
42
64.771
91.432
20.555
1.00
27.61
B


ATOM
2729
N
SER
B
43
66.547
90.680
19.422
1.00
28.99
B


ATOM
2730
CA
SER
B
43
66.611
91.946
18.672
1.00
33.61
B


ATOM
2731
CB
SER
B
43
68.012
92.565
18.722
1.00
29.97
B


ATOM
2732
OG
SER
B
43
68.478
92.606
20.047
1.00
47.21
B


ATOM
2733
C
SER
B
43
66.273
91.770
17.224
1.00
28.57
B


ATOM
2734
O
SER
B
43
66.765
90.841
16.588
1.00
32.11
B


ATOM
2735
N
LEU
B
44
65.443
92.661
16.697
1.00
26.09
B


ATOM
2736
CA
LEU
B
44
65.127
92.607
15.280
1.00
25.34
B


ATOM
2737
CB
LEU
B
44
63.620
92.609
14.987
1.00
21.75
B


ATOM
2738
CG
LEU
B
44
62.608
91.642
15.593
1.00
33.26
B


ATOM
2739
CD1
LEU
B
44
61.455
91.487
14.626
1.00
24.66
B


ATOM
2740
CD2
LEU
B
44
63.227
90.293
15.976
1.00
19.72
B


ATOM
2741
C
LEU
B
44
65.738
93.863
14.694
1.00
27.39
B


ATOM
2742
O
LEU
B
44
65.759
94.919
15.334
1.00
29.07
B


ATOM
2743
N
LEU
B
45
66.201
93.740
13.456
1.00
26.79
B


ATOM
2744
CA
LEU
B
45
66.854
94.812
12.727
1.00
26.17
B


ATOM
2745
CB
LEU
B
45
68.202
94.303
12.213
1.00
25.36
B


ATOM
2746
CG
LEU
B
45
69.343
95.281
11.950
1.00
35.90
B


ATOM
2747
CD1
LEU
B
45
70.263
94.668
10.905
1.00
31.78
B


ATOM
2748
CD2
LEU
B
45
68.853
96.627
11.519
1.00
39.62
B


ATOM
2749
C
LEU
B
45
66.016
95.198
11.516
1.00
25.11
B


ATOM
2750
O
LEU
B
45
65.568
94.320
10.761
1.00
20.94
B


ATOM
2751
N
CYS
B
46
65.803
96.487
11.298
1.00
24.85
B


ATOM
2752
CA
CYS
B
46
65.057
96.887
10.097
1.00
22.87
B


ATOM
2753
C
CYS
B
46
66.106
96.897
8.976
1.00
29.24
B


ATOM
2754
O
CYS
B
46
67.099
97.601
9.051
1.00
28.50
B


ATOM
2755
CB
CYS
B
46
64.446
98.284
10.251
1.00
26.50
B


ATOM
2756
SG
CYS
B
46
63.677
98.900
8.705
1.00
27.31
B


ATOM
2757
N
ILE
B
47
65.877
96.106
7.943
1.00
24.65
B


ATOM
2758
CA
ILE
B
47
66.817
95.991
6.842
1.00
28.64
B


ATOM
2759
CB
ILE
B
47
67.412
94.572
6.871
1.00
30.17
B


ATOM
2760
CG2
ILE
B
47
68.114
94.258
5.601
1.00
42.28
B


ATOM
2761
CG1
ILE
B
47
68.341
94.458
8.070
1.00
40.16
B


ATOM
2762
CD1
ILE
B
47
69.121
93.190
8.107
1.00
53.27
B


ATOM
2763
C
ILE
B
47
66.098
96.188
5.504
1.00
28.32
B


ATOM
2764
O
ILE
B
47
64.874
96.332
5.466
1.00
24.72
B


ATOM
2765
N
THR
B
48
66.853
96.238
4.413
1.00
24.30
B


ATOM
2766
CA
THR
B
48
66.236
96.267
3.091
1.00
27.24
B


ATOM
2767
CB
THR
B
48
66.215
97.672
2.405
1.00
31.04
B


ATOM
2768
OG1
THR
B
48
65.632
97.534
1.090
1.00
26.58
B


ATOM
2769
CG2
THR
B
48
67.627
98.250
2.271
1.00
23.59
B


ATOM
2770
C
THR
B
48
67.048
95.280
2.254
1.00
26.55
B


ATOM
2771
O
THR
B
48
68.276
95.371
2.173
1.00
27.30
B


ATOM
2772
N
LYS
B
49
66.370
94.295
1.688
1.00
24.39
B


ATOM
2773
CA
LYS
B
49
67.031
93.309
0.837
1.00
25.65
B


ATOM
2774
CB
LYS
B
49
66.323
91.943
0.940
1.00
27.44
B


ATOM
2775
CG
LYS
B
49
66.358
91.351
2.368
1.00
36.21
B


ATOM
2776
CD
LYS
B
49
67.171
90.103
2.479
1.00
40.52
B


ATOM
2777
CE
LYS
B
49
68.570
90.294
2.025
1.00
41.63
B


ATOM
2778
NZ
LYS
B
49
69.314
89.069
2.403
1.00
46.47
B


ATOM
2779
C
LYS
B
49
67.041
93.752
−0.632
1.00
33.01
B


ATOM
2780
O
LYS
B
49
67.987
93.450
−1.353
1.00
28.70
B


ATOM
2781
N
ASP
B
50
65.994
94.451
−1.083
1.00
26.02
B


ATOM
2782
CA
ASP
B
50
65.933
94.859
−2.495
1.00
29.77
B


ATOM
2783
CB
ASP
B
50
64.575
94.503
−3.108
1.00
28.99
B


ATOM
2784
CG
ASP
B
50
63.439
95.221
−2.440
1.00
26.54
B


ATOM
2785
OD1
ASP
B
50
63.720
96.043
−1.562
1.00
25.74
B


ATOM
2786
OD2
ASP
B
50
62.262
94.969
−2.779
1.00
32.42
B


ATOM
2787
C
ASP
B
50
66.200
96.327
−2.744
1.00
30.62
B


ATOM
2788
O
ASP
B
50
66.037
96.794
−3.858
1.00
24.13
B


ATOM
2789
N
LYS
B
51
66.610
97.050
−1.709
1.00
27.94
B


ATOM
2790
CA
LYS
B
51
66.889
98.473
−1.824
1.00
31.18
B


ATOM
2791
CB
LYS
B
51
68.014
98.742
−2.819
1.00
34.46
B


ATOM
2792
CG
LYS
B
51
69.381
98.355
−2.297
1.00
41.85
B


ATOM
2793
CD
LYS
B
51
70.447
98.960
−3.175
1.00
49.97
B


ATOM
2794
CE
LYS
B
51
71.826
98.869
−2.564
1.00
59.90
B


ATOM
2795
NZ
LYS
B
51
72.754
99.713
−3.366
1.00
67.70
B


ATOM
2796
C
LYS
B
51
65.666
99.305
−2.186
1.00
30.87
B


ATOM
2797
O
LYS
B
51
65.786
100.430
−2.634
1.00
28.68
B


ATOM
2798
N
VAL
B
52
64.483
98.748
−1.992
1.00
26.95
B


ATOM
2799
CA
VAL
B
52
63.263
99.501
−2.231
1.00
26.37
B


ATOM
2800
CB
VAL
B
52
62.456
98.945
−3.402
1.00
27.69
B


ATOM
2801
CG1
VAL
B
52
61.123
99.695
−3.511
1.00
32.70
B


ATOM
2802
CG2
VAL
B
52
63.236
99.113
−4.679
1.00
32.13
B


ATOM
2803
C
VAL
B
52
62.375
99.437
−1.000
1.00
30.79
B


ATOM
2804
O
VAL
B
52
61.949
100.461
−0.491
1.00
26.76
B


ATOM
2805
N
ASP
B
53
62.102
98.232
−0.512
1.00
26.86
B


ATOM
2806
CA
ASP
B
53
61.210
98.074
0.642
1.00
26.94
B


ATOM
2807
CB
ASP
B
53
60.156
96.999
0.318
1.00
42.02
B


ATOM
2808
CG
ASP
B
53
59.285
97.356
−0.871
1.00
53.53
B


ATOM
2809
OD1
ASP
B
53
58.878
98.540
−0.943
1.00
63.13
B


ATOM
2810
OD2
ASP
B
53
58.964
96.476
−1.727
1.00
64.13
B


ATOM
2811
C
ASP
B
53
62.006
97.651
1.888
1.00
25.00
B


ATOM
2812
O
ASP
B
53
63.063
97.038
1.773
1.00
22.23
B


ATOM
2813
N
GLY
B
54
61.490
97.959
3.072
1.00
20.64
B


ATOM
2814
CA
GLY
B
54
62.170
97.578
4.293
1.00
24.10
B


ATOM
2815
C
GLY
B
54
61.441
96.397
4.919
1.00
25.17
B


ATOM
2816
O
GLY
B
54
60.237
96.266
4.728
1.00
25.23
B


ATOM
2817
N
THR
B
55
62.169
95.517
5.605
1.00
23.87
B


ATOM
2818
CA
THR
B
55
61.589
94.373
6.318
1.00
20.36
B


ATOM
2819
CB
THR
B
55
61.679
93.051
5.518
1.00
27.74
B


ATOM
2820
OG1
THR
B
55
61.052
92.004
6.267
1.00
24.11
B


ATOM
2821
CG2
THR
B
55
63.151
92.669
5.212
1.00
21.92
B


ATOM
2822
C
THR
B
55
62.371
94.156
7.600
1.00
22.54
B


ATOM
2823
O
THR
B
55
63.535
94.526
7.675
1.00
24.57
B


ATOM
2824
N
TRP
B
56
61.743
93.589
8.624
1.00
23.11
B


ATOM
2825
CA
TRP
B
56
62.513
93.285
9.827
1.00
25.54
B


ATOM
2826
CB
TRP
B
56
61.580
92.954
10.988
1.00
23.18
B


ATOM
2827
CG
TRP
B
56
60.874
94.202
11.420
1.00
19.51
B


ATOM
2828
CD2
TRP
B
56
61.456
95.282
12.147
1.00
21.91
B


ATOM
2829
CE2
TRP
B
56
60.473
96.302
12.247
1.00
26.89
B


ATOM
2830
CE3
TRP
B
56
62.722
95.491
12.729
1.00
25.30
B


ATOM
2831
CD1
TRP
B
56
59.595
94.578
11.119
1.00
21.93
B


ATOM
2832
NE1
TRP
B
56
59.343
95.849
11.617
1.00
21.49
B


ATOM
2833
CZ2
TRP
B
56
60.713
97.521
12.895
1.00
26.71
B


ATOM
2834
CZ3
TRP
B
56
62.963
96.706
13.380
1.00
29.35
B


ATOM
2835
CH2
TRP
B
56
61.957
97.706
13.451
1.00
28.96
B


ATOM
2836
C
TRP
B
56
63.335
92.075
9.384
1.00
28.11
B


ATOM
2837
O
TRP
B
56
62.882
91.296
8.535
1.00
23.54
B


ATOM
2838
N
ASP
B
57
64.535
91.909
9.926
1.00
20.44
B


ATOM
2839
CA
ASP
B
57
65.401
90.812
9.470
1.00
25.81
B


ATOM
2840
CB
ASP
B
57
66.885
91.113
9.761
1.00
30.15
B


ATOM
2841
CG
ASP
B
57
67.234
91.045
11.255
1.00
35.84
B


ATOM
2842
OD1
ASP
B
57
66.319
91.161
12.102
1.00
23.31
B


ATOM
2843
OD2
ASP
B
57
68.443
90.889
11.581
1.00
41.06
B


ATOM
2844
C
ASP
B
57
65.058
89.433
9.963
1.00
26.11
B


ATOM
2845
O
ASP
B
57
65.634
88.455
9.486
1.00
28.90
B


ATOM
2846
N
LYS
B
58
64.114
89.336
10.896
1.00
29.91
B


ATOM
2847
CA
LYS
B
58
63.687
88.032
11.411
1.00
27.97
B


ATOM
2848
CB
LYS
B
58
64.697
87.491
12.443
1.00
30.61
B


ATOM
2849
CG
LYS
B
58
64.884
88.378
13.650
1.00
38.23
B


ATOM
2850
CD
LYS
B
58
65.966
87.831
14.618
1.00
41.02
B


ATOM
2851
CE
LYS
B
58
67.406
88.063
14.115
1.00
37.73
B


ATOM
2852
NZ
LYS
B
58
67.830
89.511
14.119
1.00
29.19
B


ATOM
2853
C
LYS
B
58
62.319
88.105
12.045
1.00
22.71
B


ATOM
2854
O
LYS
B
58
61.795
89.188
12.311
1.00
26.33
B


ATOM
2855
N
PRO
B
59
61.700
86.947
12.292
1.00
25.99
B


ATOM
2856
CD
PRO
B
59
62.084
85.584
11.885
1.00
24.09
B


ATOM
2857
CA
PRO
B
59
60.367
86.972
12.919
1.00
22.11
B


ATOM
2858
CB
PRO
B
59
59.902
85.515
12.845
1.00
26.86
B


ATOM
2859
CG
PRO
B
59
60.730
84.913
11.746
1.00
29.91
B


ATOM
2860
C
PRO
B
59
60.509
87.419
14.369
1.00
30.21
B


ATOM
2861
O
PRO
B
59
61.593
87.307
14.968
1.00
28.15
B


ATOM
2862
N
ALA
B
60
59.433
87.924
14.951
1.00
25.43
B


ATOM
2863
CA
ALA
B
60
59.516
88.330
16.339
1.00
26.37
B


ATOM
2864
CB
ALA
B
60
58.260
89.089
16.742
1.00
26.63
B


ATOM
2865
C
ALA
B
60
59.644
87.085
17.223
1.00
21.91
B


ATOM
2866
O
ALA
B
60
59.104
86.068
16.914
1.00
22.47
B


ATOM
2867
N
PRO
B
61
60.358
87.173
18.350
1.00
23.37
B


ATOM
2868
CD
PRO
B
61
61.134
88.320
18.870
1.00
22.24
B


ATOM
2869
CA
PRO
B
61
60.476
86.007
19.227
1.00
19.99
B


ATOM
2870
CB
PRO
B
61
61.650
86.386
20.130
1.00
21.18
B


ATOM
2871
CG
PRO
B
61
61.458
87.876
20.314
1.00
22.73
B


ATOM
2872
C
PRO
B
61
59.151
85.933
20.024
1.00
27.42
B


ATOM
2873
O
PRO
B
61
58.314
86.831
19.903
1.00
27.48
B


ATOM
2874
N
LYS
B
62
58.960
84.893
20.834
1.00
21.73
B


ATOM
2875
CA
LYS
B
62
57.737
84.770
21.648
1.00
26.90
B


ATOM
2876
CB
LYS
B
62
56.880
83.576
21.187
1.00
32.90
B


ATOM
2877
CG
LYS
B
62
56.318
83.743
19.779
1.00
39.12
B


ATOM
2878
CD
LYS
B
62
55.638
82.502
19.258
1.00
48.29
B


ATOM
2879
CE
LYS
B
62
55.142
82.741
17.828
1.00
54.55
B


ATOM
2880
NZ
LYS
B
62
54.620
81.478
17.213
1.00
59.87
B


ATOM
2881
C
LYS
B
62
58.050
84.601
23.133
1.00
30.68
B


ATOM
2882
O
LYS
B
62
59.190
84.287
23.528
1.00
24.62
B


ATOM
2883
N
CYS
B
63
57.039
84.843
23.958
1.00
25.15
B


ATOM
2884
CA
CYS
B
63
57.171
84.675
25.405
1.00
27.27
B


ATOM
2885
C
CYS
B
63
56.314
83.481
25.792
1.00
30.43
B


ATOM
2886
O
CYS
B
63
55.111
83.454
25.515
1.00
30.82
B


ATOM
2887
CB
CYS
B
63
56.646
85.903
26.149
1.00
25.60
B


ATOM
2888
SG
CYS
B
63
57.682
87.384
25.922
1.00
31.81
B


ATOM
2889
N
GLU
B
64
56.934
82.486
26.414
1.00
29.14
B


ATOM
2890
CA
GLU
B
64
56.220
81.297
26.871
1.00
27.87
B


ATOM
2891
CB
GLU
B
64
56.938
80.043
26.393
1.00
22.54
B


ATOM
2892
CG
GLU
B
64
56.839
79.836
24.884
1.00
30.23
B


ATOM
2893
CD
GLU
B
64
57.354
78.472
24.471
1.00
25.42
B


ATOM
2894
OE1
GLU
B
64
58.146
77.879
25.221
1.00
28.63
B


ATOM
2895
OE2
GLU
B
64
56.993
77.994
23.396
1.00
24.45
B


ATOM
2896
C
GLU
B
64
56.172
81.301
28.394
1.00
27.43
B


ATOM
2897
O
GLU
B
64
57.189
81.564
29.036
1.00
28.45
B


ATOM
2898
N
TYR
B
65
55.003
81.039
28.981
1.00
30.82
B


ATOM
2899
CA
TYR
B
65
54.898
81.016
30.449
1.00
26.71
B


ATOM
2900
CB
TYR
B
65
53.474
80.638
30.881
1.00
24.67
B


ATOM
2901
CG
TYR
B
65
53.222
80.754
32.382
1.00
30.61
B


ATOM
2902
CD1
TYR
B
65
53.558
81.915
33.078
1.00
37.93
B


ATOM
2903
CE1
TYR
B
65
53.369
82.013
34.464
1.00
37.10
B


ATOM
2904
CD2
TYR
B
65
52.681
79.689
33.102
1.00
29.91
B


ATOM
2905
CE2
TYR
B
65
52.481
79.765
34.477
1.00
32.04
B


ATOM
2906
CZ
TYR
B
65
52.829
80.927
35.149
1.00
38.91
B


ATOM
2907
OH
TYR
B
65
52.653
81.014
36.510
1.00
40.82
B


ATOM
2908
C
TYR
B
65
55.931
79.969
30.908
1.00
26.37
B


ATOM
2909
O
TYR
B
65
56.022
78.891
30.339
1.00
27.39
B


ATOM
2910
N
PHE
B
66
56.722
80.290
31.924
1.00
30.93
B


ATOM
2911
CA
PHE
B
66
57.770
79.381
32.362
1.00
29.14
B


ATOM
2912
CB
PHE
B
66
58.611
80.060
33.455
1.00
27.56
B


ATOM
2913
CG
PHE
B
66
59.743
79.210
33.998
1.00
28.67
B


ATOM
2914
CD1
PHE
B
66
60.632
78.559
33.139
1.00
33.68
B


ATOM
2915
CD2
PHE
B
66
59.907
79.055
35.371
1.00
30.48
B


ATOM
2916
CE1
PHE
B
66
61.669
77.763
33.648
1.00
35.23
B


ATOM
2917
CE2
PHE
B
66
60.931
78.267
35.895
1.00
26.93
B


ATOM
2918
CZ
PHE
B
66
61.816
77.618
35.041
1.00
31.25
B


ATOM
2919
C
PHE
B
66
57.283
78.013
32.844
1.00
32.09
B


ATOM
2920
O
PHE
B
66
56.382
77.924
33.671
1.00
31.84
B


ATOM
2921
N
ASN
B
67
57.882
76.956
32.312
1.00
28.91
B


ATOM
2922
CA
ASN
B
67
57.553
75.591
32.727
1.00
31.14
B


ATOM
2923
CB
ASN
B
67
57.124
74.763
31.524
1.00
32.40
B


ATOM
2924
CG
ASN
B
67
56.635
73.368
31.903
1.00
37.56
B


ATOM
2925
OD1
ASN
B
67
57.193
72.701
32.784
1.00
33.77
B


ATOM
2926
ND2
ASN
B
67
55.589
72.914
31.218
1.00
40.31
B


ATOM
2927
C
ASN
B
67
58.844
75.009
33.318
1.00
30.31
B


ATOM
2928
O
ASN
B
67
59.719
74.551
32.577
1.00
26.39
B


ATOM
2929
N
LYS
B
68
58.960
75.038
34.644
1.00
28.27
B


ATOM
2930
CA
LYS
B
68
60.148
74.535
35.329
1.00
29.00
B


ATOM
2931
CB
LYS
B
68
60.041
74.792
36.843
1.00
35.08
B


ATOM
2932
CG
LYS
B
68
58.984
73.906
37.518
1.00
41.56
B


ATOM
2933
CD
LYS
B
68
59.179
73.778
39.031
1.00
52.33
B


ATOM
2934
CE
LYS
B
68
58.907
75.077
39.764
1.00
54.82
B


ATOM
2935
NZ
LYS
B
68
58.896
74.891
41.250
1.00
58.88
B


ATOM
2936
C
LYS
B
68
60.398
73.039
35.087
1.00
32.74
B


ATOM
2937
O
LYS
B
68
61.507
72.556
35.318
1.00
32.92
B


ATOM
2938
N
TYR
B
69
59.389
72.305
34.611
1.00
33.19
B


ATOM
2939
CA
TYR
B
69
59.559
70.867
34.354
1.00
25.62
B


ATOM
2940
CB
TYR
B
69
58.259
70.096
34.687
1.00
30.97
B


ATOM
2941
CG
TYR
B
69
57.801
70.349
36.107
1.00
30.82
B


ATOM
2942
CD1
TYR
B
69
56.774
71.266
36.378
1.00
28.48
B


ATOM
2943
CE1
TYR
B
69
56.444
71.623
37.697
1.00
31.73
B


ATOM
2944
CD2
TYR
B
69
58.486
69.776
37.192
1.00
31.64
B


ATOM
2945
CE2
TYR
B
69
58.174
70.118
38.507
1.00
38.99
B


ATOM
2946
CZ
TYR
B
69
57.153
71.053
38.756
1.00
39.94
B


ATOM
2947
OH
TYR
B
69
56.901
71.467
40.048
1.00
41.68
B


ATOM
2948
C
TYR
B
69
60.029
70.516
32.955
1.00
31.61
B


ATOM
2949
O
TYR
B
69
60.549
69.414
32.722
1.00
31.53
B


ATOM
2950
N
SER
B
70
59.889
71.447
32.017
1.00
29.13
B


ATOM
2951
CA
SER
B
70
60.328
71.162
30.649
1.00
31.79
B


ATOM
2952
CB
SER
B
70
59.926
72.310
29.708
1.00
27.84
B


ATOM
2953
OG
SER
B
70
58.524
72.534
29.725
1.00
38.42
B


ATOM
2954
C
SER
B
70
61.849
70.949
30.544
1.00
34.24
B


ATOM
2955
O
SER
B
70
62.624
71.534
31.296
1.00
31.38
B


ATOM
2956
N
SER
B
71
62.261
70.097
29.613
1.00
33.64
B


ATOM
2957
CA
SER
B
71
63.676
69.832
29.351
1.00
36.09
B


ATOM
2958
CB
SER
B
71
64.265
68.809
30.324
1.00
39.34
B


ATOM
2959
OG
SER
B
71
63.823
67.519
30.007
1.00
41.01
B


ATOM
2960
C
SER
B
71
63.790
69.308
27.907
1.00
41.17
B


ATOM
2961
O
SER
B
71
62.959
68.520
27.432
1.00
41.90
B


ATOM
2962
N
CYS
B
72
64.805
69.766
27.195
1.00
32.35
B


ATOM
2963
CA
CYS
B
72
64.970
69.351
25.816
1.00
36.35
B


ATOM
2964
C
CYS
B
72
66.139
68.393
25.722
1.00
36.24
B


ATOM
2965
O
CYS
B
72
67.089
68.472
26.503
1.00
34.41
B


ATOM
2966
CB
CYS
B
72
65.205
70.586
24.920
1.00
26.09
B


ATOM
2967
SG
CYS
B
72
63.836
71.795
24.962
1.00
32.64
B


ATOM
2968
N
PRO
B
73
66.069
67.453
24.779
1.00
35.65
B


ATOM
2969
CD
PRO
B
73
64.958
67.148
23.867
1.00
38.10
B


ATOM
2970
CA
PRO
B
73
67.157
66.494
24.617
1.00
35.61
B


ATOM
2971
CB
PRO
B
73
66.567
65.461
23.661
1.00
35.75
B


ATOM
2972
CG
PRO
B
73
65.662
66.314
22.806
1.00
40.23
B


ATOM
2973
C
PRO
B
73
68.355
67.203
24.011
1.00
37.18
B


ATOM
2974
O
PRO
B
73
68.225
68.277
23.427
1.00
31.86
B


ATOM
2975
N
GLU
B
74
69.521
66.595
24.151
1.00
30.08
B


ATOM
2976
CA
GLU
B
74
70.726
67.168
23.594
1.00
36.66
B


ATOM
2977
CB
GLU
B
74
71.904
66.221
23.830
1.00
40.69
B


ATOM
2978
CG
GLU
B
74
73.123
66.500
22.972
1.00
55.61
B


ATOM
2979
CD
GLU
B
74
74.357
66.847
23.791
1.00
65.33
B


ATOM
2980
OE1
GLU
B
74
75.476
66.535
23.311
1.00
68.86
B


ATOM
2981
OE2
GLU
B
74
74.214
67.432
24.899
1.00
67.56
B


ATOM
2982
C
GLU
B
74
70.526
67.411
22.099
1.00
31.83
B


ATOM
2983
O
GLU
B
74
70.179
66.517
21.347
1.00
34.92
B


ATOM
2984
N
PRO
B
75
70.743
68.639
21.648
1.00
29.85
B


ATOM
2985
CD
PRO
B
75
71.019
69.851
22.431
1.00
29.21
B


ATOM
2986
CA
PRO
B
75
70.573
68.944
20.227
1.00
31.16
B


ATOM
2987
CB
PRO
B
75
70.349
70.441
20.235
1.00
30.77
B


ATOM
2988
CG
PRO
B
75
71.310
70.872
21.362
1.00
28.27
B


ATOM
2989
C
PRO
B
75
71.855
68.552
19.507
1.00
37.41
B


ATOM
2990
O
PRO
B
75
72.949
68.998
19.877
1.00
35.98
B


ATOM
2991
N
ILE
B
76
71.728
67.726
18.474
1.00
37.41
B


ATOM
2992
CA
ILE
B
76
72.904
67.277
17.757
1.00
41.40
B


ATOM
2993
CB
ILE
B
76
73.069
65.755
17.912
1.00
47.80
B


ATOM
2994
CG2
ILE
B
76
74.385
65.302
17.272
1.00
48.99
B


ATOM
2995
CG1
ILE
B
76
73.078
65.396
19.404
1.00
48.44
B


ATOM
2996
CD1
ILE
B
76
72.926
63.918
19.671
1.00
55.08
B


ATOM
2997
C
ILE
B
76
72.874
67.654
16.294
1.00
38.62
B


ATOM
2998
O
ILE
B
76
71.874
67.480
15.625
1.00
39.69
B


ATOM
2999
N
VAL
B
77
73.984
68.195
15.813
1.00
36.89
B


ATOM
3000
CA
VAL
B
77
74.107
68.610
14.433
1.00
38.30
B


ATOM
3001
CB
VAL
B
77
74.290
70.119
14.331
1.00
39.19
B


ATOM
3002
CG1
VAL
B
77
74.483
70.519
12.871
1.00
39.16
B


ATOM
3003
CG2
VAL
B
77
73.081
70.826
14.946
1.00
40.99
B


ATOM
3004
C
VAL
B
77
75.307
67.939
13.761
1.00
40.36
B


ATOM
3005
O
VAL
B
77
76.457
68.351
13.948
1.00
41.17
B


ATOM
3006
N
PRO
B
78
75.057
66.893
12.966
1.00
40.75
B


ATOM
3007
CD
PRO
B
78
73.785
66.267
12.590
1.00
37.39
B


ATOM
3008
CA
PRO
B
78
76.183
66.228
12.303
1.00
40.96
B


ATOM
3009
CB
PRO
B
78
75.506
65.195
11.412
1.00
41.91
B


ATOM
3010
CG
PRO
B
78
74.119
65.745
11.229
1.00
45.49
B


ATOM
3011
C
PRO
B
78
76.997
67.232
11.525
1.00
37.80
B


ATOM
3012
O
PRO
B
78
76.447
68.140
10.913
1.00
37.38
B


ATOM
3013
N
GLY
B
79
78.312
67.083
11.590
1.00
32.94
B


ATOM
3014
CA
GLY
B
79
79.186
68.002
10.900
1.00
36.40
B


ATOM
3015
C
GLY
B
79
79.427
69.261
11.712
1.00
34.31
B


ATOM
3016
O
GLY
B
79
80.209
70.109
11.304
1.00
42.13
B


ATOM
3017
N
GLY
B
80
78.760
69.404
12.855
1.00
36.96
B


ATOM
3018
CA
GLY
B
80
78.960
70.608
13.664
1.00
39.25
B


ATOM
3019
C
GLY
B
80
79.072
70.348
15.164
1.00
35.08
B


ATOM
3020
O
GLY
B
80
78.994
69.202
15.609
1.00
40.28
B


ATOM
3021
N
TYR
B
81
79.225
71.410
15.953
1.00
37.65
B


ATOM
3022
CA
TYR
B
81
79.345
71.271
17.408
1.00
33.04
B


ATOM
3023
CB
TYR
B
81
80.800
71.019
17.799
1.00
29.39
B


ATOM
3024
CG
TYR
B
81
81.745
72.112
17.331
1.00
31.26
B


ATOM
3025
CD1
TYR
B
81
82.169
73.116
18.199
1.00
28.35
B


ATOM
3026
CE1
TYR
B
81
82.992
74.143
17.766
1.00
28.17
B


ATOM
3027
CD2
TYR
B
81
82.181
72.163
16.004
1.00
28.62
B


ATOM
3028
CE2
TYR
B
81
83.006
73.188
15.559
1.00
31.47
B


ATOM
3029
CZ
TYR
B
81
83.401
74.174
16.449
1.00
30.40
B


ATOM
3030
OH
TYR
B
81
84.172
75.207
16.012
1.00
32.87
B


ATOM
3031
C
TYR
B
81
78.847
72.536
18.095
1.00
33.96
B


ATOM
3032
O
TYR
B
81
78.696
73.581
17.454
1.00
30.79
B


ATOM
3033
N
LYS
B
82
78.600
72.431
19.398
1.00
23.56
B


ATOM
3034
CA
LYS
B
82
78.114
73.557
20.195
1.00
29.94
B


ATOM
3035
CB
LYS
B
82
77.348
73.034
21.428
1.00
25.29
B


ATOM
3036
CG
LYS
B
82
76.140
72.144
21.057
1.00
27.67
B


ATOM
3037
CD
LYS
B
82
75.335
71.652
22.266
1.00
33.47
B


ATOM
3038
CE
LYS
B
82
76.215
71.041
23.365
1.00
44.83
B


ATOM
3039
NZ
LYS
B
82
77.091
69.944
22.837
1.00
45.24
B


ATOM
3040
C
LYS
B
82
79.205
74.526
20.629
1.00
29.85
B


ATOM
3041
O
LYS
B
82
80.270
74.110
21.099
1.00
31.44
B


ATOM
3042
N
ILE
B
83
78.980
75.822
20.428
1.00
25.93
B


ATOM
3043
CA
ILE
B
83
79.959
76.795
20.887
1.00
21.30
B


ATOM
3044
CB
ILE
B
83
80.449
77.771
19.800
1.00
29.03
B


ATOM
3045
CG2
ILE
B
83
81.296
77.004
18.799
1.00
23.68
B


ATOM
3046
CG1
ILE
B
83
79.278
78.514
19.155
1.00
24.89
B


ATOM
3047
CD1
ILE
B
83
79.722
79.571
18.148
1.00
27.44
B


ATOM
3048
C
ILE
B
83
79.362
77.581
22.027
1.00
26.62
B


ATOM
3049
O
ILE
B
83
80.038
78.408
22.636
1.00
32.15
B


ATOM
3050
N
ARG
B
84
78.092
77.330
22.324
1.00
24.60
B


ATOM
3051
CA
ARG
B
84
77.471
77.981
23.469
1.00
25.74
B


ATOM
3052
CB
ARG
B
84
77.047
79.396
23.131
1.00
34.80
B


ATOM
3053
CG
ARG
B
84
76.583
80.160
24.348
1.00
45.89
B


ATOM
3054
CD
ARG
B
84
76.518
81.629
24.027
1.00
56.25
B


ATOM
3055
NE
ARG
B
84
77.801
82.302
24.217
1.00
62.31
B


ATOM
3056
CZ
ARG
B
84
78.276
82.707
25.397
1.00
64.61
B


ATOM
3057
NH1
ARG
B
84
77.581
82.496
26.512
1.00
65.21
B


ATOM
3058
NH2
ARG
B
84
79.421
83.386
25.454
1.00
61.85
B


ATOM
3059
C
ARG
B
84
76.278
77.199
23.984
1.00
27.61
B


ATOM
3060
O
ARG
B
84
75.487
76.687
23.189
1.00
24.27
B


ATOM
3061
N
GLY
B
85
76.166
77.105
25.314
1.00
25.11
B


ATOM
3062
CA
GLY
B
85
75.074
76.374
25.959
1.00
23.64
B


ATOM
3063
C
GLY
B
85
75.394
74.877
26.115
1.00
32.13
B


ATOM
3064
O
GLY
B
85
75.961
74.257
25.200
1.00
25.66
B


ATOM
3065
N
SER
B
86
75.094
74.293
27.281
1.00
24.97
B


ATOM
3066
CA
SER
B
86
75.347
72.859
27.473
1.00
34.32
B


ATOM
3067
CB
SER
B
86
76.755
72.588
28.034
1.00
31.54
B


ATOM
3068
OG
SER
B
86
76.964
73.322
29.223
1.00
42.05
B


ATOM
3069
C
SER
B
86
74.315
72.304
28.415
1.00
27.65
B


ATOM
3070
O
SER
B
86
73.536
73.064
28.979
1.00
24.75
B


ATOM
3071
N
THR
B
87
74.294
70.982
28.573
1.00
27.03
B


ATOM
3072
CA
THR
B
87
73.325
70.314
29.454
1.00
29.87
B


ATOM
3073
CB
THR
B
87
73.532
68.806
29.450
1.00
34.20
B


ATOM
3074
OG1
THR
B
87
73.876
68.395
28.125
1.00
54.47
B


ATOM
3075
CG2
THR
B
87
72.263
68.107
29.856
1.00
36.26
B


ATOM
3076
C
THR
B
87
73.420
70.767
30.903
1.00
30.09
B


ATOM
3077
O
THR
B
87
74.501
71.113
31.381
1.00
30.94
B


ATOM
3078
N
PRO
B
88
72.293
70.771
31.626
1.00
26.03
B


ATOM
3079
CD
PRO
B
88
72.370
71.088
33.060
1.00
30.64
B


ATOM
3080
CA
PRO
B
88
70.921
70.406
31.240
1.00
33.13
B


ATOM
3081
CB
PRO
B
88
70.225
70.211
32.585
1.00
28.86
B


ATOM
3082
CG
PRO
B
88
70.903
71.248
33.440
1.00
31.91
B


ATOM
3083
C
PRO
B
88
70.228
71.482
30.388
1.00
31.83
B


ATOM
3084
O
PRO
B
88
70.495
72.668
30.535
1.00
29.33
B


ATOM
3085
N
TYR
B
89
69.340
71.059
29.503
1.00
27.84
B


ATOM
3086
CA
TYR
B
89
68.613
71.990
28.641
1.00
29.55
B


ATOM
3087
CB
TYR
B
89
68.553
71.414
27.239
1.00
27.73
B


ATOM
3088
CG
TYR
B
89
69.938
71.078
26.722
1.00
29.87
B


ATOM
3089
CD1
TYR
B
89
70.326
69.759
26.501
1.00
33.12
B


ATOM
3090
CE1
TYR
B
89
71.605
69.456
26.036
1.00
26.70
B


ATOM
3091
CD2
TYR
B
89
70.865
72.086
26.467
1.00
32.19
B


ATOM
3092
CE2
TYR
B
89
72.134
71.795
26.007
1.00
28.60
B


ATOM
3093
CZ
TYR
B
89
72.492
70.482
25.802
1.00
27.81
B


ATOM
3094
OH
TYR
B
89
73.770
70.211
25.415
1.00
32.86
B


ATOM
3095
C
TYR
B
89
67.212
72.242
29.176
1.00
25.88
B


ATOM
3096
O
TYR
B
89
66.333
71.400
29.025
1.00
25.96
B


ATOM
3097
N
ARG
B
90
67.015
73.405
29.790
1.00
25.05
B


ATOM
3098
CA
ARG
B
90
65.732
73.778
30.386
1.00
21.94
B


ATOM
3099
CB
ARG
B
90
65.950
74.306
31.802
1.00
25.43
B


ATOM
3100
CG
ARG
B
90
66.836
73.368
32.650
1.00
35.30
B


ATOM
3101
CD
ARG
B
90
66.308
71.933
32.684
1.00
30.92
B


ATOM
3102
NE
ARG
B
90
65.162
71.857
33.578
1.00
38.77
B


ATOM
3103
CZ
ARG
B
90
64.577
70.721
33.950
1.00
43.50
B


ATOM
3104
NH1
ARG
B
90
65.038
69.562
33.500
1.00
42.19
B


ATOM
3105
NH2
ARG
B
90
63.529
70.750
34.771
1.00
39.16
B


ATOM
3106
C
ARG
B
90
65.001
74.823
29.580
1.00
26.84
B


ATOM
3107
O
ARG
B
90
65.540
75.372
28.606
1.00
21.63
B


ATOM
3108
N
HIS
B
91
63.767
75.095
29.999
1.00
24.57
B


ATOM
3109
CA
HIS
B
91
62.903
76.057
29.318
1.00
28.69
B


ATOM
3110
CB
HIS
B
91
61.570
76.143
30.062
1.00
26.54
B


ATOM
3111
CG
HIS
B
91
60.516
76.909
29.326
1.00
34.66
B


ATOM
3112
CD2
HIS
B
91
59.414
77.563
29.770
1.00
26.97
B


ATOM
3113
ND1
HIS
B
91
60.513
77.035
27.952
1.00
29.34
B


ATOM
3114
CE1
HIS
B
91
59.457
77.735
27.582
1.00
27.63
B


ATOM
3115
NE2
HIS
B
91
58.775
78.066
28.666
1.00
34.45
B


ATOM
3116
C
HIS
B
91
63.546
77.445
29.177
1.00
30.22
B


ATOM
3117
O
HIS
B
91
63.937
78.069
30.161
1.00
23.98
B


ATOM
3118
N
GLY
B
92
63.663
77.915
27.941
1.00
26.25
B


ATOM
3119
CA
GLY
B
92
64.260
79.209
27.702
1.00
20.48
B


ATOM
3120
C
GLY
B
92
65.752
79.118
27.458
1.00
24.40
B


ATOM
3121
O
GLY
B
92
66.310
80.102
27.019
1.00
24.34
B


ATOM
3122
N
ASP
B
93
66.407
77.974
27.724
1.00
20.35
B


ATOM
3123
CA
ASP
B
93
67.857
77.884
27.472
1.00
19.73
B


ATOM
3124
CB
ASP
B
93
68.487
76.575
27.992
1.00
23.61
B


ATOM
3125
CG
ASP
B
93
68.579
76.504
29.504
1.00
20.60
B


ATOM
3126
OD1
ASP
B
93
68.336
77.522
30.167
1.00
23.89
B


ATOM
3127
OD2
ASP
B
93
68.884
75.401
30.020
1.00
25.06
B


ATOM
3128
C
ASP
B
93
68.138
77.911
25.981
1.00
25.02
B


ATOM
3129
O
ASP
B
93
67.362
77.415
25.170
1.00
24.06
B


ATOM
3130
N
SER
B
94
69.298
78.422
25.625
1.00
21.77
B


ATOM
3131
CA
SER
B
94
69.641
78.511
24.229
1.00
27.05
B


ATOM
3132
CB
SER
B
94
69.779
79.972
23.834
1.00
28.57
B


ATOM
3133
OG
SER
B
94
70.417
80.035
22.587
1.00
38.84
B


ATOM
3134
C
SER
B
94
70.935
77.815
23.908
1.00
25.71
B


ATOM
3135
O
SER
B
94
71.833
77.727
24.753
1.00
26.52
B


ATOM
3136
N
VAL
B
95
71.051
77.331
22.678
1.00
27.36
B


ATOM
3137
CA
VAL
B
95
72.291
76.689
22.245
1.00
26.56
B


ATOM
3138
CB
VAL
B
95
72.096
75.178
22.088
1.00
26.82
B


ATOM
3139
CG1
VAL
B
95
73.282
74.581
21.344
1.00
34.06
B


ATOM
3140
CG2
VAL
B
95
71.908
74.549
23.460
1.00
30.69
B


ATOM
3141
C
VAL
B
95
72.729
77.273
20.908
1.00
30.07
B


ATOM
3142
O
VAL
B
95
71.903
77.496
20.019
1.00
26.03
B


ATOM
3143
N
THR
B
96
74.026
77.526
20.758
1.00
27.23
B


ATOM
3144
CA
THR
B
96
74.540
78.058
19.503
1.00
23.14
B


ATOM
3145
CB
THR
B
96
75.242
79.419
19.687
1.00
32.77
B


ATOM
3146
OG1
THR
B
96
74.292
80.383
20.163
1.00
26.94
B


ATOM
3147
CG2
THR
B
96
75.845
79.896
18.329
1.00
26.13
B


ATOM
3148
C
THR
B
96
75.550
77.071
18.915
1.00
33.62
B


ATOM
3149
O
THR
B
96
76.445
76.614
19.618
1.00
26.19
B


ATOM
3150
N
PHE
B
97
75.381
76.732
17.638
1.00
28.29
B


ATOM
3151
CA
PHE
B
97
76.249
75.788
16.922
1.00
27.82
B


ATOM
3152
CB
PHE
B
97
75.409
74.849
16.039
1.00
27.23
B


ATOM
3153
CG
PHE
B
97
74.598
73.858
16.796
1.00
30.99
B


ATOM
3154
CD1
PHE
B
97
73.325
74.183
17.257
1.00
28.10
B


ATOM
3155
CD2
PHE
B
97
75.149
72.633
17.153
1.00
24.15
B


ATOM
3156
CE1
PHE
B
97
72.608
73.310
18.081
1.00
29.54
B


ATOM
3157
CE2
PHE
B
97
74.434
71.756
17.975
1.00
26.96
B


ATOM
3158
CZ
PHE
B
97
73.155
72.101
18.444
1.00
28.76
B


ATOM
3159
C
PHE
B
97
77.247
76.479
15.980
1.00
33.87
B


ATOM
3160
O
PHE
B
97
77.088
77.663
15.628
1.00
30.90
B


ATOM
3161
N
ALA
B
98
78.274
75.725
15.582
1.00
33.98
B


ATOM
3162
CA
ALA
B
98
79.266
76.152
14.574
1.00
34.14
B


ATOM
3163
CB
ALA
B
98
80.551
76.651
15.211
1.00
38.55
B


ATOM
3164
C
ALA
B
98
79.544
74.880
13.785
1.00
36.15
B


ATOM
3165
O
ALA
B
98
79.370
73.775
14.312
1.00
35.98
B


ATOM
3166
N
CYS
B
99
79.953
75.018
12.527
1.00
38.50
B


ATOM
3167
CA
CYS
B
99
80.266
73.843
11.711
1.00
39.15
B


ATOM
3168
C
CYS
B
99
81.747
73.512
11.779
1.00
36.00
B


ATOM
3169
O
CYS
B
99
82.573
74.408
11.865
1.00
32.17
B


ATOM
3170
CB
CYS
B
99
79.901
74.086
10.251
1.00
42.12
B


ATOM
3171
SG
CYS
B
99
78.108
74.162
9.949
1.00
40.52
B


ATOM
3172
N
LYS
B
100
82.089
72.229
11.749
1.00
35.70
B


ATOM
3173
CA
LYS
B
100
83.498
71.842
11.770
1.00
40.35
B


ATOM
3174
CB
LYS
B
100
83.632
70.319
11.848
1.00
43.13
B


ATOM
3175
CG
LYS
B
100
83.185
69.707
13.148
1.00
38.59
B


ATOM
3176
CD
LYS
B
100
82.771
68.263
12.966
1.00
44.87
B


ATOM
3177
CE
LYS
B
100
82.689
67.562
14.314
1.00
48.06
B


ATOM
3178
NZ
LYS
B
100
82.353
66.122
14.196
1.00
53.59
B


ATOM
3179
C
LYS
B
100
84.190
72.329
10.482
1.00
45.56
B


ATOM
3180
O
LYS
B
100
83.532
72.770
9.525
1.00
42.57
B


ATOM
3181
N
THR
B
101
85.519
72.239
10.456
1.00
47.39
B


ATOM
3182
CA
THR
B
101
86.286
72.651
9.282
1.00
48.55
B


ATOM
3183
CB
THR
B
101
87.794
72.408
9.497
1.00
48.03
B


ATOM
3184
OG1
THR
B
101
88.281
73.285
10.521
1.00
47.14
B


ATOM
3185
CG2
THR
B
101
88.557
72.683
8.212
1.00
51.80
B


ATOM
3186
C
THR
B
101
85.816
71.876
8.042
1.00
47.72
B


ATOM
3187
O
THR
B
101
85.523
70.681
8.127
1.00
43.56
B


ATOM
3188
N
ASN
B
102
85.747
72.555
6.901
1.00
47.11
B


ATOM
3189
CA
ASN
B
102
85.294
71.943
5.647
1.00
48.36
B


ATOM
3190
CB
ASN
B
102
85.961
70.594
5.375
1.00
53.67
B


ATOM
3191
CG
ASN
B
102
87.461
70.703
5.219
1.00
58.65
B


ATOM
3192
OD1
ASN
B
102
87.980
71.763
4.856
1.00
59.24
B


ATOM
3193
ND2
ASN
B
102
88.171
69.599
5.484
1.00
61.19
B


ATOM
3194
C
ASN
B
102
83.797
71.728
5.632
1.00
49.36
B


ATOM
3195
O
ASN
B
102
83.284
70.965
4.814
1.00
51.85
B


ATOM
3196
N
PHE
B
103
83.097
72.378
6.555
1.00
48.85
B


ATOM
3197
CA
PHE
B
103
81.641
72.294
6.617
1.00
44.54
B


ATOM
3198
CB
PHE
B
103
81.179
71.539
7.855
1.00
44.01
B


ATOM
3199
CG
PHE
B
103
81.335
70.067
7.740
1.00
45.42
B


ATOM
3200
CD1
PHE
B
103
82.554
69.461
8.037
1.00
47.36
B


ATOM
3201
CD2
PHE
B
103
80.270
69.274
7.323
1.00
44.68
B


ATOM
3202
CE1
PHE
B
103
82.715
68.064
7.921
1.00
44.91
B


ATOM
3203
CE2
PHE
B
103
80.413
67.883
7.202
1.00
46.13
B


ATOM
3204
CZ
PHE
B
103
81.638
67.276
7.503
1.00
44.51
B


ATOM
3205
C
PHE
B
103
81.103
73.700
6.663
1.00
43.79
B


ATOM
3206
O
PHE
B
103
81.736
74.577
7.239
1.00
41.42
B


ATOM
3207
N
SER
B
104
79.939
73.908
6.052
1.00
46.45
B


ATOM
3208
CA
SER
B
104
79.306
75.216
6.007
1.00
47.47
B


ATOM
3209
CB
SER
B
104
79.176
75.706
4.570
1.00
53.61
B


ATOM
3210
OG
SER
B
104
77.886
75.379
4.057
1.00
61.85
B


ATOM
3211
C
SER
B
104
77.921
75.051
6.596
1.00
46.27
B


ATOM
3212
O
SER
B
104
77.285
74.007
6.425
1.00
46.36
B


ATOM
3213
N
MET
B
105
77.435
76.089
7.269
1.00
47.80
B


ATOM
3214
CA
MET
B
105
76.129
75.998
7.913
1.00
50.16
B


ATOM
3215
CB
MET
B
105
76.114
76.848
9.184
1.00
42.24
B


ATOM
3216
CG
MET
B
105
74.783
76.829
9.931
1.00
39.50
B


ATOM
3217
SD
MET
B
105
74.951
77.585
11.559
1.00
31.98
B


ATOM
3218
CE
MET
B
105
76.153
76.456
12.300
1.00
32.14
B


ATOM
3219
C
MET
B
105
74.948
76.398
7.046
1.00
52.75
B


ATOM
3220
O
MET
B
105
75.066
77.234
6.147
1.00
52.67
B


ATOM
3221
N
ASN
B
106
73.807
75.787
7.343
1.00
57.15
B


ATOM
3222
CA
ASN
B
106
72.560
76.072
6.664
1.00
56.25
B


ATOM
3223
CB
ASN
B
106
72.186
74.950
5.722
1.00
63.78
B


ATOM
3224
CG
ASN
B
106
72.678
75.210
4.332
1.00
72.25
B


ATOM
3225
OD1
ASN
B
106
72.787
76.372
3.916
1.00
78.63
B


ATOM
3226
ND2
ASN
B
106
72.995
74.141
3.597
1.00
74.20
B


ATOM
3227
C
ASN
B
106
71.504
76.200
7.727
1.00
54.17
B


ATOM
3228
O
ASN
B
106
71.303
75.277
8.508
1.00
56.24
B


ATOM
3229
N
GLY
B
107
70.833
77.342
7.762
1.00
50.67
B


ATOM
3230
CA
GLY
B
107
69.805
77.552
8.763
1.00
44.02
B


ATOM
3231
C
GLY
B
107
70.334
78.414
9.886
1.00
42.66
B


ATOM
3232
O
GLY
B
107
71.478
78.864
9.854
1.00
41.68
B


ATOM
3233
N
ASN
B
108
69.500
78.646
10.889
1.00
46.24
B


ATOM
3234
CA
ASN
B
108
69.878
79.464
12.032
1.00
47.08
B


ATOM
3235
CB
ASN
B
108
68.613
79.910
12.751
1.00
55.35
B


ATOM
3236
CG
ASN
B
108
67.793
80.851
11.903
1.00
63.30
B


ATOM
3237
OD1
ASN
B
108
68.164
82.013
11.722
1.00
67.44
B


ATOM
3238
ND2
ASN
B
108
66.691
80.352
11.346
1.00
68.11
B


ATOM
3239
C
ASN
B
108
70.808
78.723
12.981
1.00
39.04
B


ATOM
3240
O
ASN
B
108
70.560
77.577
13.347
1.00
33.47
B


ATOM
3241
N
LYS
B
109
71.858
79.406
13.402
1.00
34.53
B


ATOM
3242
CA
LYS
B
109
72.859
78.806
14.280
1.00
35.55
B


ATOM
3243
CB
LYS
B
109
74.138
79.632
14.217
1.00
35.53
B


ATOM
3244
CG
LYS
B
109
73.909
81.037
14.698
1.00
37.21
B


ATOM
3245
CD
LYS
B
109
75.176
81.691
15.171
1.00
43.67
B


ATOM
3246
CE
LYS
B
109
74.913
83.141
15.501
1.00
53.84
B


ATOM
3247
NZ
LYS
B
109
76.079
83.848
16.124
1.00
67.33
B


ATOM
3248
C
LYS
B
109
72.456
78.639
15.753
1.00
33.59
B


ATOM
3249
O
LYS
B
109
73.116
77.900
16.490
1.00
32.99
B


ATOM
3250
N
SER
B
110
71.409
79.337
16.198
1.00
29.02
B


ATOM
3251
CA
SER
B
110
70.972
79.239
17.597
1.00
31.62
B


ATOM
3252
CB
SER
B
110
71.068
80.588
18.298
1.00
28.39
B


ATOM
3253
OG
SER
B
110
72.395
81.073
18.276
1.00
35.28
B


ATOM
3254
C
SER
B
110
69.547
78.730
17.734
1.00
33.04
B


ATOM
3255
O
SER
B
110
68.665
79.049
16.935
1.00
28.18
B


ATOM
3256
N
VAL
B
111
69.336
77.927
18.761
1.00
32.94
B


ATOM
3257
CA
VAL
B
111
68.028
77.360
19.032
1.00
28.12
B


ATOM
3258
CB
VAL
B
111
67.998
75.911
18.507
1.00
34.53
B


ATOM
3259
CG1
VAL
B
111
69.117
75.074
19.143
1.00
27.59
B


ATOM
3260
CG2
VAL
B
111
66.694
75.304
18.785
1.00
41.47
B


ATOM
3261
C
VAL
B
111
67.729
77.488
20.539
1.00
26.65
B


ATOM
3262
O
VAL
B
111
68.631
77.621
21.359
1.00
27.71
B


ATOM
3263
N
TRP
B
112
66.447
77.496
20.866
1.00
26.33
B


ATOM
3264
CA
TRP
B
112
65.932
77.654
22.205
1.00
21.46
B


ATOM
3265
CB
TRP
B
112
65.023
78.895
22.287
1.00
27.43
B


ATOM
3266
CG
TRP
B
112
65.772
80.142
22.392
1.00
28.72
B


ATOM
3267
CD2
TRP
B
112
66.455
80.818
21.323
1.00
27.13
B


ATOM
3268
CE2
TRP
B
112
67.130
81.922
21.894
1.00
23.65
B


ATOM
3269
CE3
TRP
B
112
66.560
80.595
19.944
1.00
27.06
B


ATOM
3270
CD1
TRP
B
112
66.053
80.849
23.542
1.00
29.24
B


ATOM
3271
NE1
TRP
B
112
66.875
81.916
23.242
1.00
22.18
B


ATOM
3272
CZ2
TRP
B
112
67.896
82.804
21.132
1.00
24.55
B


ATOM
3273
CZ3
TRP
B
112
67.328
81.476
19.179
1.00
32.51
B


ATOM
3274
CH2
TRP
B
112
67.985
82.570
19.782
1.00
28.74
B


ATOM
3275
C
TRP
B
112
65.113
76.462
22.622
1.00
24.09
B


ATOM
3276
O
TRP
B
112
64.348
75.924
21.826
1.00
23.03
B


ATOM
3277
N
CYS
B
113
65.257
76.065
23.886
1.00
16.00
B


ATOM
3278
CA
CYS
B
113
64.507
74.942
24.421
1.00
22.66
B


ATOM
3279
C
CYS
B
113
63.090
75.442
24.775
1.00
27.37
B


ATOM
3280
O
CYS
B
113
62.892
76.248
25.702
1.00
23.71
B


ATOM
3281
CB
CYS
B
113
65.230
74.386
25.636
1.00
24.38
B


ATOM
3282
SG
CYS
B
113
64.295
73.084
26.474
1.00
25.70
B


ATOM
3283
N
GLN
B
114
62.105
74.973
24.014
1.00
22.77
B


ATOM
3284
CA
GLN
B
114
60.704
75.406
24.190
1.00
27.53
B


ATOM
3285
CB
GLN
B
114
59.993
75.312
22.852
1.00
23.87
B


ATOM
3286
CG
GLN
B
114
60.669
76.101
21.776
1.00
25.80
B


ATOM
3287
CD
GLN
B
114
59.934
75.957
20.455
1.00
34.33
B


ATOM
3288
OE1
GLN
B
114
59.540
74.850
20.084
1.00
40.82
B


ATOM
3289
NE2
GLN
B
114
59.749
77.062
19.744
1.00
28.51
B


ATOM
3290
C
GLN
B
114
59.918
74.632
25.242
1.00
24.17
B


ATOM
3291
O
GLN
B
114
60.324
73.557
25.638
1.00
24.70
B


ATOM
3292
N
ALA
B
115
58.806
75.187
25.704
1.00
28.17
B


ATOM
3293
CA
ALA
B
115
57.984
74.520
26.720
1.00
32.79
B


ATOM
3294
CB
ALA
B
115
56.769
75.392
27.068
1.00
30.00
B


ATOM
3295
C
ALA
B
115
57.523
73.107
26.316
1.00
34.03
B


ATOM
3296
O
ALA
B
115
57.338
72.237
27.170
1.00
33.21
B


ATOM
3297
N
ASN
B
116
57.343
72.881
25.020
1.00
36.70
B


ATOM
3298
CA
ASN
B
116
56.913
71.573
24.526
1.00
38.02
B


ATOM
3299
CB
ASN
B
116
56.230
71.728
23.168
1.00
34.90
B


ATOM
3300
CG
ASN
B
116
57.198
72.144
22.087
1.00
33.42
B


ATOM
3301
OD1
ASN
B
116
58.359
72.454
22.364
1.00
28.93
B


ATOM
3302
ND2
ASN
B
116
56.728
72.167
20.853
1.00
30.98
B


ATOM
3303
C
ASN
B
116
58.104
70.608
24.392
1.00
34.77
B


ATOM
3304
O
ASN
B
116
58.017
69.612
23.695
1.00
37.59
B


ATOM
3305
N
ASN
B
117
59.219
70.937
25.044
1.00
36.92
B


ATOM
3306
CA
ASN
B
117
60.425
70.094
25.072
1.00
31.01
B


ATOM
3307
CB
ASN
B
117
60.095
68.728
25.699
1.00
35.95
B


ATOM
3308
CG
ASN
B
117
59.327
68.855
27.022
1.00
41.69
B


ATOM
3309
OD1
ASN
B
117
58.165
68.448
27.116
1.00
48.82
B


ATOM
3310
ND2
ASN
B
117
59.966
69.428
28.041
1.00
38.48
B


ATOM
3311
C
ASN
B
117
61.155
69.913
23.753
1.00
35.04
B


ATOM
3312
O
ASN
B
117
61.989
69.010
23.584
1.00
30.97
B


ATOM
3313
N
MET
B
118
60.877
70.804
22.814
1.00
32.90
B


ATOM
3314
CA
MET
B
118
61.544
70.747
21.539
1.00
30.69
B


ATOM
3315
CB
MET
B
118
60.533
70.486
20.426
1.00
39.19
B


ATOM
3316
CG
MET
B
118
60.143
69.004
20.284
1.00
45.35
B


ATOM
3317
SD
MET
B
118
58.844
68.904
19.065
1.00
61.98
B


ATOM
3318
CE
MET
B
118
57.379
68.930
20.152
1.00
59.12
B


ATOM
3319
C
MET
B
118
62.299
72.033
21.290
1.00
32.74
B


ATOM
3320
O
MET
B
118
61.958
73.093
21.820
1.00
31.41
B


ATOM
3321
N
TRP
B
119
63.322
71.913
20.460
1.00
30.27
B


ATOM
3322
CA
TRP
B
119
64.197
73.007
20.122
1.00
32.04
B


ATOM
3323
CB
TRP
B
119
65.519
72.477
19.556
1.00
29.45
B


ATOM
3324
CG
TRP
B
119
66.377
71.888
20.618
1.00
34.08
B


ATOM
3325
CD2
TRP
B
119
67.091
72.613
21.638
1.00
29.55
B


ATOM
3326
CE2
TRP
B
119
67.641
71.653
22.514
1.00
32.16
B


ATOM
3327
CE3
TRP
B
119
67.309
73.974
21.891
1.00
27.61
B


ATOM
3328
CD1
TRP
B
119
66.533
70.563
20.907
1.00
27.66
B


ATOM
3329
NE1
TRP
B
119
67.287
70.419
22.045
1.00
29.97
B


ATOM
3330
CZ2
TRP
B
119
68.401
72.018
23.634
1.00
33.69
B


ATOM
3331
CZ3
TRP
B
119
68.067
74.333
23.002
1.00
28.67
B


ATOM
3332
CH2
TRP
B
119
68.603
73.356
23.860
1.00
29.17
B


ATOM
3333
C
TRP
B
119
63.672
74.059
19.188
1.00
35.37
B


ATOM
3334
O
TRP
B
119
62.946
73.750
18.264
1.00
34.01
B


ATOM
3335
N
GLY
B
120
64.129
75.279
19.507
1.00
44.82
B


ATOM
3336
CA
GLY
B
120
63.928
76.577
18.868
1.00
40.39
B


ATOM
3337
C
GLY
B
120
62.736
76.818
18.037
1.00
45.27
B


ATOM
3338
O
GLY
B
120
61.922
75.920
17.884
1.00
54.52
B


ATOM
3339
N
PRO
B
121
62.570
78.049
17.525
1.00
39.72
B


ATOM
3340
CD
PRO
B
121
63.331
79.268
17.805
1.00
34.64
B


ATOM
3341
CA
PRO
B
121
61.411
78.339
16.678
1.00
40.69
B


ATOM
3342
CB
PRO
B
121
61.355
79.869
16.674
1.00
33.54
B


ATOM
3343
CG
PRO
B
121
62.781
80.221
16.775
1.00
38.11
B


ATOM
3344
C
PRO
B
121
61.717
77.733
15.294
1.00
38.17
B


ATOM
3345
O
PRO
B
121
60.817
77.305
14.591
1.00
40.16
B


ATOM
3346
N
THR
B
122
62.994
77.693
14.930
1.00
33.84
B


ATOM
3347
CA
THR
B
122
63.424
77.113
13.660
1.00
38.91
B


ATOM
3348
CB
THR
B
122
64.548
77.939
12.988
1.00
42.26
B


ATOM
3349
OG1
THR
B
122
65.803
77.671
13.634
1.00
39.47
B


ATOM
3350
CG2
THR
B
122
64.259
79.431
13.106
1.00
40.58
B


ATOM
3351
C
THR
B
122
63.968
75.708
13.874
1.00
40.61
B


ATOM
3352
O
THR
B
122
64.163
75.266
14.999
1.00
43.52
B


ATOM
3353
N
ARG
B
123
64.220
75.005
12.784
1.00
42.70
B


ATOM
3354
CA
ARG
B
123
64.762
73.656
12.870
1.00
46.43
B


ATOM
3355
CB
ARG
B
123
64.505
72.912
11.549
1.00
48.19
B


ATOM
3356
CG
ARG
B
123
64.849
71.433
11.600
1.00
63.34
B


ATOM
3357
CD
ARG
B
123
64.109
70.638
10.517
1.00
71.72
B


ATOM
3358
NE
ARG
B
123
62.664
70.564
10.762
1.00
75.72
B


ATOM
3359
CZ
ARG
B
123
61.734
70.934
9.881
1.00
77.53
B


ATOM
3360
NH1
ARG
B
123
62.096
71.410
8.690
1.00
78.13
B


ATOM
3361
NH2
ARG
B
123
60.444
70.820
10.184
1.00
75.29
B


ATOM
3362
C
ARG
B
123
66.270
73.774
13.141
1.00
36.14
B


ATOM
3363
O
ARG
B
123
66.861
74.816
12.880
1.00
34.06
B


ATOM
3364
N
LEU
B
124
66.888
72.714
13.659
1.00
36.62
B


ATOM
3365
CA
LEU
B
124
68.326
72.743
13.939
1.00
35.68
B


ATOM
3366
CB
LEU
B
124
68.818
71.410
14.509
1.00
37.22
B


ATOM
3367
CG
LEU
B
124
68.399
71.019
15.933
1.00
38.68
B


ATOM
3368
CD1
LEU
B
124
69.008
69.670
16.342
1.00
37.12
B


ATOM
3369
CD2
LEU
B
124
68.860
72.097
16.874
1.00
34.15
B


ATOM
3370
C
LEU
B
124
69.081
73.029
12.656
1.00
37.07
B


ATOM
3371
O
LEU
B
124
68.701
72.584
11.583
1.00
35.87
B


ATOM
3372
N
PRO
B
125
70.168
73.787
12.750
1.00
40.36
B


ATOM
3373
CD
PRO
B
125
70.832
74.306
13.962
1.00
38.05
B


ATOM
3374
CA
PRO
B
125
70.933
74.085
11.538
1.00
35.47
B


ATOM
3375
CB
PRO
B
125
71.993
75.069
12.029
1.00
35.51
B


ATOM
3376
CG
PRO
B
125
72.224
74.641
13.460
1.00
35.57
B


ATOM
3377
C
PRO
B
125
71.535
72.794
11.002
1.00
40.45
B


ATOM
3378
O
PRO
B
125
71.432
71.739
11.640
1.00
33.92
B


ATOM
3379
N
THR
B
126
72.131
72.867
9.814
1.00
41.58
B


ATOM
3380
CA
THR
B
126
72.765
71.704
9.217
1.00
41.03
B


ATOM
3381
CB
THR
B
126
71.929
71.137
8.006
1.00
42.99
B


ATOM
3382
OG1
THR
B
126
71.625
72.182
7.075
1.00
45.10
B


ATOM
3383
CG2
THR
B
126
70.628
70.529
8.503
1.00
40.83
B


ATOM
3384
C
THR
B
126
74.157
72.116
8.777
1.00
41.16
B


ATOM
3385
O
THR
B
126
74.415
73.299
8.544
1.00
40.35
B


ATOM
3386
N
CYS
B
127
75.074
71.158
8.716
1.00
42.86
B


ATOM
3387
CA
CYS
B
127
76.433
71.468
8.279
1.00
48.44
B


ATOM
3388
C
CYS
B
127
76.774
70.561
7.098
1.00
53.76
B


ATOM
3389
O
CYS
B
127
76.711
69.336
7.219
1.00
55.59
B


ATOM
3390
CB
CYS
B
127
77.440
71.249
9.422
1.00
49.29
B


ATOM
3391
SG
CYS
B
127
77.365
72.471
10.779
1.00
41.21
B


ATOM
3392
N
VAL
B
128
77.117
71.159
5.960
1.00
52.99
B


ATOM
3393
CA
VAL
B
128
77.466
70.386
4.764
1.00
58.11
B


ATOM
3394
CB
VAL
B
128
76.479
70.674
3.612
1.00
60.17
B


ATOM
3395
CG1
VAL
B
128
76.356
72.179
3.395
1.00
59.61
B


ATOM
3396
CG2
VAL
B
128
76.974
70.005
2.322
1.00
62.78
B


ATOM
3397
C
VAL
B
128
78.881
70.738
4.290
1.00
55.96
B


ATOM
3398
O
VAL
B
128
79.307
71.875
4.427
1.00
56.76
B


ATOM
3399
N
SER
B
129
79.604
69.763
3.747
1.00
58.88
B


ATOM
3400
CA
SER
B
129
80.976
69.974
3.241
1.00
63.04
B


ATOM
3401
CB
SER
B
129
81.489
68.685
2.616
1.00
64.42
B


ATOM
3402
OG
SER
B
129
80.566
68.249
1.633
1.00
74.77
B


ATOM
3403
C
SER
B
129
81.061
71.092
2.194
1.00
60.01
B


ATOM
3404
O
SER
B
129
82.054
71.856
2.166
1.00
59.63
B


ATOM
3405
OXT
SER
B
129
80.121
71.165
1.381
1.00
61.43
B


ATOM
3406
CB
ALA
C
1
51.630
81.929
2.879
1.00
35.79
C


ATOM
3407
C
ALA
C
1
50.853
84.288
2.366
1.00
35.46
C


ATOM
3408
O
ALA
C
1
50.053
85.212
2.282
1.00
40.99
C


ATOM
3409
N
ALA
C
1
49.558
82.473
1.552
1.00
39.80
C


ATOM
3410
CA
ALA
C
1
50.392
82.883
2.708
1.00
38.04
C


ATOM
3411
N
ILE
C
2
52.138
84.436
2.113
1.00
25.56
C


ATOM
3412
CA
ILE
C
2
52.684
85.736
1.810
1.00
32.00
C


ATOM
3413
CB
ILE
C
2
53.982
85.933
2.591
1.00
24.33
C


ATOM
3414
CG2
ILE
C
2
54.794
87.063
1.974
1.00
24.84
C


ATOM
3415
CG1
ILE
C
2
53.647
86.140
4.085
1.00
31.72
C


ATOM
3416
CD1
ILE
C
2
54.875
86.286
5.004
1.00
28.23
C


ATOM
3417
C
ILE
C
2
52.955
85.930
0.331
1.00
29.98
C


ATOM
3418
O
ILE
C
2
53.280
84.966
−0.364
1.00
24.28
C


ATOM
3419
N
SER
C
3
52.816
87.164
−0.156
1.00
25.13
C


ATOM
3420
CA
SER
C
3
53.130
87.453
−1.564
1.00
24.56
C


ATOM
3421
CB
SER
C
3
51.848
87.773
−2.360
1.00
23.32
C


ATOM
3422
OG
SER
C
3
51.206
88.949
−1.866
1.00
28.18
C


ATOM
3423
C
SER
C
3
54.111
88.630
−1.651
1.00
28.79
C


ATOM
3424
O
SER
C
3
54.240
89.413
−0.703
1.00
25.00
C


ATOM
3425
N
CYS
C
4
54.834
88.720
−2.766
1.00
24.82
C


ATOM
3426
CA
CYS
C
4
55.760
89.824
−3.003
1.00
23.87
C


ATOM
3427
C
CYS
C
4
55.114
90.751
−4.020
1.00
26.63
C


ATOM
3428
O
CYS
C
4
54.488
90.277
−4.974
1.00
24.86
C


ATOM
3429
CB
CYS
C
4
57.075
89.341
−3.595
1.00
26.41
C


ATOM
3430
SG
CYS
C
4
58.182
88.516
−2.412
1.00
26.19
C


ATOM
3431
N
GLY
C
5
55.270
92.056
−3.843
1.00
25.01
C


ATOM
3432
CA
GLY
C
5
54.697
93.002
−4.800
1.00
23.78
C


ATOM
3433
C
GLY
C
5
55.467
93.100
−6.115
1.00
33.19
C


ATOM
3434
O
GLY
C
5
56.444
92.346
−6.350
1.00
24.73
C


ATOM
3435
N
SER
C
6
55.020
94.068
−6.975
1.00
24.24
C


ATOM
3436
CA
SER
C
6
55.654
94.257
−8.285
1.00
31.29
C


ATOM
3437
CB
SER
C
6
54.994
95.528
−8.834
1.00
28.12
C


ATOM
3438
OG
SER
C
6
53.739
95.485
−9.097
1.00
30.57
C


ATOM
3439
C
SER
C
6
57.187
94.422
−8.106
1.00
28.51
C


ATOM
3440
O
SER
C
6
57.681
95.162
−7.262
1.00
27.78
C


ATOM
3441
N
PRO
C
7
57.921
93.562
−8.849
1.00
24.89
C


ATOM
3442
CD
PRO
C
7
57.432
92.669
−9.919
1.00
29.34
C


ATOM
3443
CA
PRO
C
7
59.391
93.554
−8.765
1.00
29.21
C


ATOM
3444
CB
PRO
C
7
59.778
92.308
−9.570
1.00
30.50
C


ATOM
3445
CG
PRO
C
7
58.721
92.286
−10.660
1.00
34.19
C


ATOM
3446
C
PRO
C
7
60.066
94.829
−9.286
1.00
25.82
C


ATOM
3447
O
PRO
C
7
59.490
95.586
−10.054
1.00
30.82
C


ATOM
3448
N
PRO
C
8
61.312
95.071
−8.879
1.00
25.55
C


ATOM
3449
CD
PRO
C
8
62.239
94.221
−8.107
1.00
22.99
C


ATOM
3450
CA
PRO
C
8
61.972
96.293
−9.360
1.00
27.11
C


ATOM
3451
CB
PRO
C
8
63.315
96.292
−8.613
1.00
23.33
C


ATOM
3452
CG
PRO
C
8
63.116
95.255
−7.464
1.00
30.29
C


ATOM
3453
C
PRO
C
8
62.162
96.260
−10.877
1.00
27.57
C


ATOM
3454
O
PRO
C
8
62.478
95.206
−11.457
1.00
27.35
C


ATOM
3455
N
PRO
C
9
61.945
97.401
−11.552
1.00
28.23
C


ATOM
3456
CD
PRO
C
9
61.527
98.718
−11.049
1.00
30.53
C


ATOM
3457
CA
PRO
C
9
62.116
97.415
−13.009
1.00
29.41
C


ATOM
3458
CB
PRO
C
9
61.430
98.714
−13.417
1.00
30.12
C


ATOM
3459
CG
PRO
C
9
61.742
99.607
−12.272
1.00
31.67
C


ATOM
3460
C
PRO
C
9
63.607
97.389
−13.404
1.00
37.14
C


ATOM
3461
O
PRO
C
9
64.487
97.661
−12.572
1.00
30.31
C


ATOM
3462
N
ILE
C
10
63.883
97.070
−14.669
1.00
30.28
C


ATOM
3463
CA
ILE
C
10
65.252
97.015
−15.166
1.00
29.72
C


ATOM
3464
CB
ILE
C
10
65.704
95.533
−15.351
1.00
30.40
C


ATOM
3465
CG2
ILE
C
10
64.708
94.766
−16.273
1.00
30.23
C


ATOM
3466
CG1
ILE
C
10
67.121
95.474
−15.931
1.00
28.32
C


ATOM
3467
CD1
ILE
C
10
67.729
94.058
−15.907
1.00
27.53
C


ATOM
3468
C
ILE
C
10
65.380
97.792
−16.488
1.00
36.89
C


ATOM
3469
O
ILE
C
10
64.732
97.472
−17.491
1.00
34.25
C


ATOM
3470
N
LEU
C
11
66.219
98.822
−16.483
1.00
38.68
C


ATOM
3471
CA
LEU
C
11
66.436
99.629
−17.678
1.00
36.69
C


ATOM
3472
CB
LEU
C
11
67.269
100.846
−17.320
1.00
40.95
C


ATOM
3473
CG
LEU
C
11
66.804
102.189
−17.855
1.00
45.07
C


ATOM
3474
CD1
LEU
C
11
65.484
102.575
−17.199
1.00
43.54
C


ATOM
3475
CD2
LEU
C
11
67.875
103.215
−17.565
1.00
43.98
C


ATOM
3476
C
LEU
C
11
67.176
98.837
−18.758
1.00
39.52
C


ATOM
3477
O
LEU
C
11
68.193
98.204
−18.475
1.00
37.78
C


ATOM
3478
N
ASN
C
12
66.680
98.899
−19.988
1.00
30.25
C


ATOM
3479
CA
ASN
C
12
67.284
98.227
−21.121
1.00
28.03
C


ATOM
3480
CB
ASN
C
12
68.705
98.746
−21.351
1.00
38.34
C


ATOM
3481
CG
ASN
C
12
68.739
100.231
−21.653
1.00
42.68
C


ATOM
3482
OD1
ASN
C
12
69.139
101.036
−20.813
1.00
47.15
C


ATOM
3483
ND2
ASN
C
12
68.318
100.596
−22.859
1.00
44.31
C


ATOM
3484
C
ASN
C
12
67.294
96.721
−20.910
1.00
36.66
C


ATOM
3485
O
ASN
C
12
68.253
96.075
−21.417
1.00
38.52
C


ATOM
3486
N
GLY
C
13
66.330
96.216
−20.179
1.00
37.43
C


ATOM
3487
CA
GLY
C
13
66.219
94.783
−19.993
1.00
37.37
C


ATOM
3488
C
GLY
C
13
64.776
94.380
−19.800
1.00
34.87
C


ATOM
3489
O
GLY
C
13
63.859
95.180
−19.928
1.00
37.34
C


ATOM
3490
N
ARG
C
14
64.557
93.119
−19.493
1.00
35.59
C


ATOM
3491
CA
ARG
C
14
63.201
92.681
−19.259
1.00
36.84
C


ATOM
3492
CB
ARG
C
14
62.562
92.191
−20.558
1.00
41.62
C


ATOM
3493
CG
ARG
C
14
63.338
91.087
−21.221
1.00
51.45
C


ATOM
3494
CD
ARG
C
14
63.739
91.505
−22.634
1.00
65.10
C


ATOM
3495
NE
ARG
C
14
64.692
92.626
−22.649
1.00
73.47
C


ATOM
3496
CZ
ARG
C
14
64.669
93.630
−23.528
1.00
76.84
C


ATOM
3497
NH1
ARG
C
14
63.736
93.667
−24.477
1.00
76.76
C


ATOM
3498
NH2
ARG
C
14
65.576
94.600
−23.457
1.00
76.89
C


ATOM
3499
C
ARG
C
14
63.179
91.573
−18.224
1.00
34.90
C


ATOM
3500
O
ARG
C
14
64.211
90.974
−17.875
1.00
30.74
C


ATOM
3501
N
ILE
C
15
61.985
91.308
−17.725
1.00
32.52
C


ATOM
3502
CA
ILE
C
15
61.809
90.274
−16.740
1.00
35.90
C


ATOM
3503
CB
ILE
C
15
61.048
90.814
−15.525
1.00
31.54
C


ATOM
3504
CG2
ILE
C
15
60.749
89.677
−14.553
1.00
25.41
C


ATOM
3505
CG1
ILE
C
15
61.862
91.952
−14.893
1.00
30.46
C


ATOM
3506
CD1
ILE
C
15
61.086
92.836
−13.959
1.00
28.46
C


ATOM
3507
C
ILE
C
15
61.013
89.156
−17.357
1.00
39.71
C


ATOM
3508
O
ILE
C
15
60.004
89.403
−18.022
1.00
39.51
C


ATOM
3509
N
SER
C
16
61.469
87.932
−17.136
1.00
39.22
C


ATOM
3510
CA
SER
C
16
60.762
86.750
−17.623
1.00
50.26
C


ATOM
3511
CB
SER
C
16
61.410
85.472
−17.077
1.00
53.90
C


ATOM
3512
OG
SER
C
16
61.213
85.384
−15.655
1.00
59.12
C


ATOM
3513
C
SER
C
16
59.326
86.801
−17.091
1.00
50.15
C


ATOM
3514
O
SER
C
16
59.097
87.096
−15.911
1.00
46.35
C


ATOM
3515
N
TYR
C
17
58.377
86.475
−17.961
1.00
54.91
C


ATOM
3516
CA
TYR
C
17
56.951
86.453
−17.629
1.00
50.96
C


ATOM
3517
CB
TYR
C
17
56.154
85.806
−18.765
1.00
56.78
C


ATOM
3518
CG
TYR
C
17
54.665
86.055
−18.678
1.00
58.05
C


ATOM
3519
CD1
TYR
C
17
54.109
87.224
−19.209
1.00
59.99
C


ATOM
3520
CE1
TYR
C
17
52.751
87.509
−19.071
1.00
61.22
C


ATOM
3521
CD2
TYR
C
17
53.822
85.163
−18.004
1.00
55.94
C


ATOM
3522
CE2
TYR
C
17
52.457
85.438
−17.854
1.00
58.29
C


ATOM
3523
CZ
TYR
C
17
51.926
86.620
−18.395
1.00
61.48
C


ATOM
3524
OH
TYR
C
17
50.577
86.917
−18.272
1.00
63.66
C


ATOM
3525
C
TYR
C
17
56.708
85.652
−16.360
1.00
49.21
C


ATOM
3526
O
TYR
C
17
57.228
84.555
−16.189
1.00
51.47
C


ATOM
3527
N
TYR
C
18
55.921
86.212
−15.459
1.00
49.68
C


ATOM
3528
CA
TYR
C
18
55.600
85.528
−14.218
1.00
46.63
C


ATOM
3529
CB
TYR
C
18
56.361
86.154
−13.033
1.00
42.78
C


ATOM
3530
CG
TYR
C
18
56.242
87.663
−12.969
1.00
35.46
C


ATOM
3531
CD1
TYR
C
18
57.129
88.488
−13.678
1.00
36.33
C


ATOM
3532
CE1
TYR
C
18
56.996
89.880
−13.663
1.00
33.08
C


ATOM
3533
CD2
TYR
C
18
55.220
88.270
−12.243
1.00
29.58
C


ATOM
3534
CE2
TYR
C
18
55.083
89.665
−12.218
1.00
29.35
C


ATOM
3535
CZ
TYR
C
18
55.968
90.465
−12.921
1.00
27.89
C


ATOM
3536
OH
TYR
C
18
55.863
91.850
−12.860
1.00
32.49
C


ATOM
3537
C
TYR
C
18
54.100
85.695
−14.022
1.00
43.03
C


ATOM
3538
O
TYR
C
18
53.496
86.659
−14.513
1.00
41.84
C


ATOM
3539
N
SER
C
19
53.496
84.758
−13.314
1.00
44.03
C


ATOM
3540
CA
SER
C
19
52.067
84.849
−13.068
1.00
49.05
C


ATOM
3541
CB
SER
C
19
51.430
83.455
−13.036
1.00
48.59
C


ATOM
3542
OG
SER
C
19
52.115
82.585
−12.147
1.00
50.14
C


ATOM
3543
C
SER
C
19
51.823
85.584
−11.749
1.00
50.35
C


ATOM
3544
O
SER
C
19
52.661
85.567
−10.839
1.00
46.39
C


ATOM
3545
N
THR
C
20
50.607
86.109
−11.537
1.00
52.04
C


ATOM
3546
CA
THR
C
20
50.099
86.975
−10.488
1.00
53.58
C


ATOM
3547
CB
THR
C
20
49.571
88.293
−11.054
1.00
55.84
C


ATOM
3548
OG1
THR
C
20
48.476
88.030
−11.940
1.00
60.69
C


ATOM
3549
CG2
THR
C
20
50.671
89.029
−11.802
1.00
52.60
C


ATOM
3550
C
THR
C
20
49.003
86.277
−9.703
1.00
56.21
C


ATOM
3551
O
THR
C
20
48.037
85.783
−10.339
1.00
59.04
C


ATOM
3552
N
PRO
C
21
49.034
86.244
−8.386
1.00
47.82
C


ATOM
3553
CD
PRO
C
21
48.004
85.901
−7.826
1.00
47.40
C


ATOM
3554
CA
PRO
C
21
50.027
86.916
−7.519
1.00
42.99
C


ATOM
3555
CB
PRO
C
21
49.335
86.932
−6.151
1.00
43.36
C


ATOM
3556
CG
PRO
C
21
48.022
86.266
−6.380
1.00
47.21
C


ATOM
3557
C
PRO
C
21
51.356
86.153
−7.464
1.00
39.76
C


ATOM
3558
O
PRO
C
21
51.481
85.058
−8.071
1.00
37.13
C


ATOM
3559
N
ILE
C
22
52.324
86.813
−6.805
1.00
33.73
C


ATOM
3560
CA
ILE
C
22
53.646
86.216
−6.676
1.00
31.36
C


ATOM
3561
CB
ILE
C
22
54.766
87.216
−6.985
1.00
33.33
C


ATOM
3562
CG2
ILE
C
22
56.125
86.572
−6.755
1.00
33.08
C


ATOM
3563
CG1
ILE
C
22
54.641
87.724
−8.423
1.00
24.06
C


ATOM
3564
CD1
ILE
C
22
55.565
88.876
−8.747
1.00
24.35
C


ATOM
3565
C
ILE
C
22
53.824
85.628
−5.269
1.00
34.54
C


ATOM
3566
O
ILE
C
22
53.977
86.348
−4.285
1.00
28.13
C


ATOM
3567
N
ALA
C
23
53.864
84.299
−5.155
1.00
28.78
C


ATOM
3568
CA
ALA
C
23
54.048
83.695
−3.836
1.00
30.59
C


ATOM
3569
CB
ALA
C
23
53.437
82.316
−3.834
1.00
31.70
C


ATOM
3570
C
ALA
C
23
55.517
83.599
−3.412
1.00
30.12
C


ATOM
3571
O
ALA
C
23
56.399
83.517
−4.266
1.00
25.80
C


ATOM
3572
N
VAL
C
24
55.784
83.613
−2.102
1.00
32.21
C


ATOM
3573
CA
VAL
C
24
57.165
83.456
−1.625
1.00
30.00
C


ATOM
3574
CB
VAL
C
24
57.272
83.290
−0.087
1.00
35.52
C


ATOM
3575
CG1
VAL
C
24
56.808
84.568
0.597
1.00
35.78
C


ATOM
3576
CG2
VAL
C
24
56.459
82.108
0.378
1.00
39.11
C


ATOM
3577
C
VAL
C
24
57.673
82.175
−2.253
1.00
31.34
C


ATOM
3578
O
VAL
C
24
56.918
81.231
−2.413
1.00
28.53
C


ATOM
3579
N
GLY
C
25
58.947
82.128
−2.603
1.00
30.39
C


ATOM
3580
CA
GLY
C
25
59.456
80.936
−3.249
1.00
32.92
C


ATOM
3581
C
GLY
C
25
59.526
81.119
−4.764
1.00
34.28
C


ATOM
3582
O
GLY
C
25
60.245
80.389
−5.443
1.00
37.66
C


ATOM
3583
N
THR
C
26
58.785
82.086
−5.296
1.00
25.38
C


ATOM
3584
CA
THR
C
26
58.791
82.337
−6.735
1.00
33.97
C


ATOM
3585
CB
THR
C
26
57.652
83.304
−7.169
1.00
27.23
C


ATOM
3586
OG1
THR
C
26
56.390
82.761
−6.784
1.00
35.09
C


ATOM
3587
CG2
THR
C
26
57.650
83.514
−8.696
1.00
27.35
C


ATOM
3588
C
THR
C
26
60.113
82.985
−7.132
1.00
35.06
C


ATOM
3589
O
THR
C
26
60.614
83.888
−6.441
1.00
32.34
C


ATOM
3590
N
VAL
C
27
60.662
82.523
−8.246
1.00
27.52
C


ATOM
3591
CA
VAL
C
27
61.914
83.046
−8.795
1.00
30.48
C


ATOM
3592
CB
VAL
C
27
62.901
81.912
−9.116
1.00
35.60
C


ATOM
3593
CG1
VAL
C
27
64.064
82.451
−9.936
1.00
34.59
C


ATOM
3594
CG2
VAL
C
27
63.398
81.269
−7.830
1.00
34.12
C


ATOM
3595
C
VAL
C
27
61.632
83.751
−10.114
1.00
32.83
C


ATOM
3596
O
VAL
C
27
60.908
83.216
−10.953
1.00
31.75
C


ATOM
3597
N
ILE
C
28
62.168
84.956
−10.310
1.00
31.95
C


ATOM
3598
CA
ILE
C
28
61.967
85.628
−11.600
1.00
25.76
C


ATOM
3599
CB
ILE
C
28
61.234
86.962
−11.482
1.00
25.19
C


ATOM
3600
CG2
ILE
C
28
59.782
86.710
−11.057
1.00
27.07
C


ATOM
3601
CG1
ILE
C
28
61.981
87.915
−10.530
1.00
20.91
C


ATOM
3602
CD1
ILE
C
28
61.363
89.345
−10.512
1.00
24.89
C


ATOM
3603
C
ILE
C
28
63.341
85.870
−12.187
1.00
29.97
C


ATOM
3604
O
ILE
C
28
64.341
85.934
−11.450
1.00
24.78
C


ATOM
3605
N
ARG
C
29
63.394
86.015
−13.505
1.00
31.68
C


ATOM
3606
CA
ARG
C
29
64.673
86.191
−14.185
1.00
33.25
C


ATOM
3607
CB
ARG
C
29
64.962
84.984
−15.093
1.00
43.21
C


ATOM
3608
CG
ARG
C
29
65.539
83.751
−14.395
1.00
55.79
C


ATOM
3609
CD
ARG
C
29
64.795
82.458
−14.794
1.00
63.30
C


ATOM
3610
NE
ARG
C
29
63.873
82.000
−13.745
1.00
66.73
C


ATOM
3611
CZ
ARG
C
29
62.570
81.762
−13.917
1.00
68.37
C


ATOM
3612
NH1
ARG
C
29
62.003
81.935
−15.119
1.00
66.91
C


ATOM
3613
NH2
ARG
C
29
61.831
81.353
−12.883
1.00
61.65
C


ATOM
3614
C
ARG
C
29
64.794
87.444
−15.020
1.00
30.28
C


ATOM
3615
O
ARG
C
29
63.956
87.732
−15.872
1.00
29.65
C


ATOM
3616
N
TYR
C
30
65.867
88.181
−14.778
1.00
32.96
C


ATOM
3617
CA
TYR
C
30
66.159
89.400
−15.527
1.00
31.51
C


ATOM
3618
CB
TYR
C
30
66.759
90.437
−14.590
1.00
27.74
C


ATOM
3619
CG
TYR
C
30
65.773
91.001
−13.617
1.00
23.76
C


ATOM
3620
CD1
TYR
C
30
65.257
90.224
−12.571
1.00
28.24
C


ATOM
3621
CE1
TYR
C
30
64.350
90.773
−11.631
1.00
28.16
C


ATOM
3622
CD2
TYR
C
30
65.360
92.327
−13.718
1.00
27.82
C


ATOM
3623
CE2
TYR
C
30
64.456
92.879
−12.793
1.00
28.53
C


ATOM
3624
CZ
TYR
C
30
63.971
92.097
−11.758
1.00
22.02
C


ATOM
3625
OH
TYR
C
30
63.160
92.703
−10.848
1.00
24.79
C


ATOM
3626
C
TYR
C
30
67.161
89.133
−16.641
1.00
25.91
C


ATOM
3627
O
TYR
C
30
68.007
88.254
−16.520
1.00
26.19
C


ATOM
3628
N
SER
C
31
67.065
89.883
−17.731
1.00
28.15
C


ATOM
3629
CA
SER
C
31
68.027
89.740
−18.811
1.00
28.99
C


ATOM
3630
CB
SER
C
31
67.611
88.643
−19.795
1.00
25.70
C


ATOM
3631
OG
SER
C
31
66.381
88.981
−20.380
1.00
32.21
C


ATOM
3632
C
SER
C
31
68.124
91.082
−19.518
1.00
30.27
C


ATOM
3633
O
SER
C
31
67.213
91.910
−19.437
1.00
31.27
C


ATOM
3634
N
CYS
C
32
69.238
91.304
−20.192
1.00
27.74
C


ATOM
3635
CA
CYS
C
32
69.457
92.558
−20.890
1.00
32.43
C


ATOM
3636
C
CYS
C
32
69.378
92.286
−22.369
1.00
36.47
C


ATOM
3637
O
CYS
C
32
69.611
91.146
−22.819
1.00
33.53
C


ATOM
3638
CB
CYS
C
32
70.840
93.110
−20.530
1.00
30.11
C


ATOM
3639
SG
CYS
C
32
70.999
93.505
−18.757
1.00
30.29
C


ATOM
3640
N
SER
C
33
68.922
93.383
−23.026
1.00
41.81
C


ATOM
3641
CA
SER
C
33
69.024
93.272
−24.485
1.00
48.46
C


ATOM
3642
CB
SER
C
33
68.136
94.544
−24.975
1.00
51.34
C


ATOM
3643
OG
SER
C
33
68.752
95.734
−24.524
1.00
57.23
C


ATOM
3644
C
SER
C
33
70.449
92.848
−24.842
1.00
54.01
C


ATOM
3645
O
SER
C
33
71.347
92.852
−23.989
1.00
61.22
C


ATOM
3646
N
GLY
C
34
70.633
92.487
−26.096
1.00
55.17
C


ATOM
3647
CA
GLY
C
34
71.928
91.989
−26.558
1.00
49.56
C


ATOM
3648
C
GLY
C
34
73.161
92.877
−26.748
1.00
47.38
C


ATOM
3649
O
GLY
C
34
74.279
92.377
−26.942
1.00
46.07
C


ATOM
3650
N
THR
C
35
72.832
94.184
−26.690
1.00
41.74
C


ATOM
3651
CA
THR
C
35
73.932
95.134
−26.777
1.00
46.36
C


ATOM
3652
CB
THR
C
35
73.603
96.329
−27.720
1.00
51.23
C


ATOM
3653
OG1
THR
C
35
72.301
96.848
−27.419
1.00
57.47
C


ATOM
3654
CG2
THR
C
35
73.641
95.881
−29.190
1.00
54.03
C


ATOM
3655
C
THR
C
35
74.305
95.658
−25.383
1.00
43.55
C


ATOM
3656
O
THR
C
35
75.001
96.655
−25.258
1.00
40.41
C


ATOM
3657
N
PHE
C
36
73.840
94.969
−24.342
1.00
34.64
C


ATOM
3658
CA
PHE
C
36
74.133
95.342
−22.965
1.00
31.17
C


ATOM
3659
CB
PHE
C
36
72.888
95.857
−22.253
1.00
30.28
C


ATOM
3660
CG
PHE
C
36
72.414
97.176
−22.750
1.00
32.70
C


ATOM
3661
CD1
PHE
C
36
71.692
97.266
−23.944
1.00
36.40
C


ATOM
3662
CD2
PHE
C
36
72.729
98.343
−22.054
1.00
33.13
C


ATOM
3663
CE1
PHE
C
36
71.301
98.500
−24.427
1.00
37.59
C


ATOM
3664
CE2
PHE
C
36
72.345
99.588
−22.528
1.00
32.44
C


ATOM
3665
CZ
PHE
C
36
71.629
99.673
−23.718
1.00
38.52
C


ATOM
3666
C
PHE
C
36
74.612
94.107
−22.239
1.00
34.05
C


ATOM
3667
O
PHE
C
36
74.432
93.001
−22.727
1.00
31.93
C


ATOM
3668
N
ARG
C
37
75.236
94.292
−21.079
1.00
28.78
C


ATOM
3669
CA
ARG
C
37
75.703
93.167
−20.295
1.00
27.36
C


ATOM
3670
CB
ARG
C
37
77.233
93.140
−20.235
1.00
27.49
C


ATOM
3671
CG
ARG
C
37
77.871
92.816
−21.587
1.00
21.08
C


ATOM
3672
CD
ARG
C
37
77.403
91.440
−22.049
1.00
27.97
C


ATOM
3673
NE
ARG
C
37
78.050
91.042
−23.301
1.00
35.17
C


ATOM
3674
CZ
ARG
C
37
77.641
91.427
−24.503
1.00
36.96
C


ATOM
3675
NH1
ARG
C
37
76.572
92.213
−24.620
1.00
32.52
C


ATOM
3676
NH2
ARG
C
37
78.308
91.039
−25.581
1.00
37.15
C


ATOM
3677
C
ARG
C
37
75.091
93.309
−18.913
1.00
29.60
C


ATOM
3678
O
ARG
C
37
75.060
94.400
−18.336
1.00
25.97
C


ATOM
3679
N
LEU
C
38
74.614
92.193
−18.383
1.00
25.39
C


ATOM
3680
CA
LEU
C
38
73.952
92.202
−17.083
1.00
27.27
C


ATOM
3681
CB
LEU
C
38
73.038
90.984
−16.980
1.00
27.65
C


ATOM
3682
CG
LEU
C
38
72.221
90.971
−15.692
1.00
35.28
C


ATOM
3683
CD1
LEU
C
38
70.799
91.354
−16.019
1.00
33.06
C


ATOM
3684
CD2
LEU
C
38
72.278
89.589
−15.051
1.00
40.58
C


ATOM
3685
C
LEU
C
38
74.951
92.203
−15.926
1.00
24.03
C


ATOM
3686
O
LEU
C
38
75.851
91.376
−15.892
1.00
27.79
C


ATOM
3687
N
ILE
C
39
74.794
93.146
−15.001
1.00
25.30
C


ATOM
3688
CA
ILE
C
39
75.654
93.264
−13.819
1.00
26.06
C


ATOM
3689
CB
ILE
C
39
76.255
94.707
−13.686
1.00
31.18
C


ATOM
3690
CG2
ILE
C
39
77.130
94.813
−12.455
1.00
28.29
C


ATOM
3691
CG1
ILE
C
39
77.057
95.071
−14.955
1.00
37.49
C


ATOM
3692
CD1
ILE
C
39
78.059
94.022
−15.415
1.00
46.78
C


ATOM
3693
C
ILE
C
39
74.787
92.979
−12.590
1.00
27.54
C


ATOM
3694
O
ILE
C
39
73.807
93.683
−12.327
1.00
21.11
C


ATOM
3695
N
GLY
C
40
75.140
91.950
−11.829
1.00
28.46
C


ATOM
3696
CA
GLY
C
40
74.351
91.619
−10.654
1.00
28.45
C


ATOM
3697
C
GLY
C
40
73.691
90.275
−10.901
1.00
31.11
C


ATOM
3698
O
GLY
C
40
73.762
89.752
−12.008
1.00
27.47
C


ATOM
3699
N
GLU
C
41
73.057
89.713
−9.876
1.00
32.89
C


ATOM
3700
CA
GLU
C
41
72.395
88.411
−9.994
1.00
37.70
C


ATOM
3701
CB
GLU
C
41
72.006
87.900
−8.597
1.00
35.85
C


ATOM
3702
CG
GLU
C
41
71.217
86.601
−8.624
1.00
47.53
C


ATOM
3703
CD
GLU
C
41
71.983
85.447
−9.235
1.00
48.84
C


ATOM
3704
OE1
GLU
C
41
71.419
84.733
−10.088
1.00
44.21
C


ATOM
3705
OE2
GLU
C
41
73.153
85.252
−8.842
1.00
56.76
C


ATOM
3706
C
GLU
C
41
71.161
88.510
−10.893
1.00
32.10
C


ATOM
3707
O
GLU
C
41
70.336
89.404
−10.718
1.00
31.37
C


ATOM
3708
N
LYS
C
42
71.033
87.604
−11.857
1.00
33.31
C


ATOM
3709
CA
LYS
C
42
69.879
87.647
−12.760
1.00
33.67
C


ATOM
3710
CB
LYS
C
42
70.207
86.955
−14.069
1.00
33.01
C


ATOM
3711
CG
LYS
C
42
70.550
85.515
−13.891
1.00
39.88
C


ATOM
3712
CD
LYS
C
42
70.862
84.895
−15.238
1.00
43.23
C


ATOM
3713
CE
LYS
C
42
71.922
83.820
−15.105
1.00
48.14
C


ATOM
3714
NZ
LYS
C
42
71.648
82.872
−13.965
1.00
52.44
C


ATOM
3715
C
LYS
C
42
68.591
87.038
−12.185
1.00
34.36
C


ATOM
3716
O
LYS
C
42
67.509
87.306
−12.687
1.00
37.69
C


ATOM
3717
N
SER
C
43
68.685
86.265
−11.117
1.00
25.52
C


ATOM
3718
CA
SER
C
43
67.472
85.664
−10.565
1.00
31.67
C


ATOM
3719
CB
SER
C
43
67.668
84.155
−10.331
1.00
28.31
C


ATOM
3720
OG
SER
C
43
67.869
83.464
−11.537
1.00
39.90
C


ATOM
3721
C
SER
C
43
67.099
86.274
−9.233
1.00
29.32
C


ATOM
3722
O
SER
C
43
67.945
86.318
−8.342
1.00
31.30
C


ATOM
3723
N
LEU
C
44
65.853
86.735
−9.087
1.00
27.40
C


ATOM
3724
CA
LEU
C
44
65.393
87.267
−7.808
1.00
28.37
C


ATOM
3725
CB
LEU
C
44
64.744
88.638
−7.931
1.00
27.60
C


ATOM
3726
CG
LEU
C
44
65.498
89.876
−8.393
1.00
38.75
C


ATOM
3727
CD1
LEU
C
44
64.700
91.149
−7.896
1.00
26.06
C


ATOM
3728
CD2
LEU
C
44
66.913
89.846
−7.907
1.00
39.16
C


ATOM
3729
C
LEU
C
44
64.352
86.315
−7.218
1.00
29.49
C


ATOM
3730
O
LEU
C
44
63.469
85.796
−7.914
1.00
35.15
C


ATOM
3731
N
LEU
C
45
64.434
86.137
−5.919
1.00
25.55
C


ATOM
3732
CA
LEU
C
45
63.560
85.222
−5.230
1.00
28.43
C


ATOM
3733
CB
LEU
C
45
64.412
84.210
−4.469
1.00
28.67
C


ATOM
3734
CG
LEU
C
45
63.709
83.269
−3.493
1.00
30.62
C


ATOM
3735
CD1
LEU
C
45
62.896
82.195
−4.244
1.00
32.56
C


ATOM
3736
CD2
LEU
C
45
64.798
82.623
−2.649
1.00
31.55
C


ATOM
3737
C
LEU
C
45
62.664
85.961
−4.263
1.00
28.71
C


ATOM
3738
O
LEU
C
45
63.094
86.894
−3.558
1.00
21.68
C


ATOM
3739
N
CYS
C
46
61.410
85.547
−4.238
1.00
25.91
C


ATOM
3740
CA
CYS
C
46
60.455
86.152
−3.343
1.00
26.90
C


ATOM
3741
C
CYS
C
46
60.610
85.392
−2.032
1.00
30.25
C


ATOM
3742
O
CYS
C
46
60.448
84.173
−1.980
1.00
28.51
C


ATOM
3743
CB
CYS
C
46
59.021
86.026
−3.908
1.00
28.02
C


ATOM
3744
SG
CYS
C
46
57.741
86.546
−2.696
1.00
28.63
C


ATOM
3745
N
ILE
C
47
60.925
86.115
−0.971
1.00
21.11
C


ATOM
3746
CA
ILE
C
47
61.120
85.484
0.314
1.00
24.65
C


ATOM
3747
CB
ILE
C
47
62.619
85.501
0.722
1.00
32.62
C


ATOM
3748
CG2
ILE
C
47
63.505
84.878
−0.358
1.00
27.06
C


ATOM
3749
CG1
ILE
C
47
63.050
86.958
0.959
1.00
25.64
C


ATOM
3750
CD1
ILE
C
47
64.429
87.119
1.592
1.00
32.03
C


ATOM
3751
C
ILE
C
47
60.431
86.274
1.421
1.00
31.05
C


ATOM
3752
O
ILE
C
47
59.931
87.399
1.226
1.00
22.58
C


ATOM
3753
N
THR
C
48
60.421
85.669
2.596
1.00
27.58
C


ATOM
3754
CA
THR
C
48
59.964
86.388
3.764
1.00
27.51
C


ATOM
3755
CB
THR
C
48
58.584
85.987
4.273
1.00
28.85
C


ATOM
3756
OG1
THR
C
48
58.303
86.778
5.426
1.00
25.48
C


ATOM
3757
CG2
THR
C
48
58.490
84.526
4.619
1.00
26.50
C


ATOM
3758
C
THR
C
48
61.001
86.164
4.839
1.00
29.22
C


ATOM
3759
O
THR
C
48
61.455
85.049
5.060
1.00
29.40
C


ATOM
3760
N
LYS
C
49
61.445
87.243
5.463
1.00
27.29
C


ATOM
3761
CA
LYS
C
49
62.425
87.112
6.522
1.00
31.22
C


ATOM
3762
CB
LYS
C
49
63.478
88.220
6.427
1.00
26.53
C


ATOM
3763
CG
LYS
C
49
64.229
88.239
5.134
1.00
35.41
C


ATOM
3764
CD
LYS
C
49
65.703
88.351
5.346
1.00
40.12
C


ATOM
3765
CE
LYS
C
49
66.262
87.134
6.055
1.00
42.32
C


ATOM
3766
NZ
LYS
C
49
67.729
87.262
6.166
1.00
46.64
C


ATOM
3767
C
LYS
C
49
61.745
87.219
7.877
1.00
33.53
C


ATOM
3768
O
LYS
C
49
62.249
86.692
8.856
1.00
30.36
C


ATOM
3769
N
ASP
C
50
60.596
87.896
7.952
1.00
25.34
C


ATOM
3770
CA
ASP
C
50
59.978
88.083
9.253
1.00
26.98
C


ATOM
3771
CB
ASP
C
50
59.961
89.579
9.597
1.00
25.16
C


ATOM
3772
CG
ASP
C
50
59.154
90.397
8.607
1.00
27.41
C


ATOM
3773
OD1
ASP
C
50
58.459
89.768
7.749
1.00
22.49
C


ATOM
3774
OD2
ASP
C
50
59.207
91.664
8.693
1.00
26.37
C


ATOM
3775
C
ASP
C
50
58.598
87.492
9.365
1.00
25.28
C


ATOM
3776
O
ASP
C
50
57.883
87.770
10.321
1.00
24.69
C


ATOM
3777
N
LYS
C
51
58.247
86.664
8.386
1.00
26.19
C


ATOM
3778
CA
LYS
C
51
56.948
86.006
8.319
1.00
23.97
C


ATOM
3779
CB
LYS
C
51
56.717
85.091
9.526
1.00
31.09
C


ATOM
3780
CG
LYS
C
51
57.749
84.021
9.749
1.00
32.84
C


ATOM
3781
CD
LYS
C
51
57.625
82.919
8.769
1.00
44.41
C


ATOM
3782
CE
LYS
C
51
58.505
81.752
9.179
1.00
52.55
C


ATOM
3783
NZ
LYS
C
51
58.266
81.397
10.605
1.00
51.40
C


ATOM
3784
C
LYS
C
51
55.791
86.996
8.241
1.00
25.78
C


ATOM
3785
O
LYS
C
51
54.660
86.628
8.510
1.00
25.03
C


ATOM
3786
N
VAL
C
52
56.048
88.252
7.896
1.00
25.78
C


ATOM
3787
CA
VAL
C
52
54.937
89.195
7.758
1.00
23.31
C


ATOM
3788
CB
VAL
C
52
54.976
90.286
8.894
1.00
30.82
C


ATOM
3789
CG1
VAL
C
52
53.786
91.197
8.802
1.00
27.39
C


ATOM
3790
CG2
VAL
C
52
54.946
89.611
10.264
1.00
33.79
C


ATOM
3791
C
VAL
C
52
55.085
89.832
6.376
1.00
29.16
C


ATOM
3792
O
VAL
C
52
54.193
89.764
5.537
1.00
25.28
C


ATOM
3793
N
ASP
C
53
56.248
90.433
6.146
1.00
31.64
C


ATOM
3794
CA
ASP
C
53
56.575
91.099
4.892
1.00
27.94
C


ATOM
3795
CB
ASP
C
53
57.647
92.167
5.150
1.00
28.37
C


ATOM
3796
CG
ASP
C
53
57.189
93.235
6.132
1.00
30.66
C


ATOM
3797
OD1
ASP
C
53
56.323
94.069
5.744
1.00
20.78
C


ATOM
3798
OD2
ASP
C
53
57.700
93.245
7.287
1.00
27.56
C


ATOM
3799
C
ASP
C
53
57.112
90.126
3.837
1.00
23.86
C


ATOM
3800
O
ASP
C
53
57.862
89.212
4.164
1.00
24.61
C


ATOM
3801
N
GLY
C
54
56.735
90.327
2.573
1.00
26.63
C


ATOM
3802
CA
GLY
C
54
57.253
89.489
1.476
1.00
20.87
C


ATOM
3803
C
GLY
C
54
58.190
90.420
0.730
1.00
25.47
C


ATOM
3804
O
GLY
C
54
57.814
91.563
0.435
1.00
19.97
C


ATOM
3805
N
THR
C
55
59.422
89.999
0.459
1.00
22.42
C


ATOM
3806
CA
THR
C
55
60.345
90.907
−0.247
1.00
21.92
C


ATOM
3807
CB
THR
C
55
61.326
91.591
0.760
1.00
24.17
C


ATOM
3808
OG1
THR
C
55
61.999
92.669
0.106
1.00
24.68
C


ATOM
3809
CG2
THR
C
55
62.401
90.597
1.251
1.00
24.06
C


ATOM
3810
C
THR
C
55
61.184
90.153
−1.284
1.00
22.33
C


ATOM
3811
O
THR
C
55
61.298
88.929
−1.209
1.00
24.76
C


ATOM
3812
N
TRP
C
56
61.767
90.882
−2.230
1.00
22.04
C


ATOM
3813
CA
TRP
C
56
62.637
90.281
−3.226
1.00
21.23
C


ATOM
3814
CB
TRP
C
56
62.689
91.142
−4.506
1.00
28.51
C


ATOM
3815
CG
TRP
C
56
61.377
91.081
−5.223
1.00
25.63
C


ATOM
3816
CD2
TRP
C
56
60.853
89.962
−5.970
1.00
26.11
C


ATOM
3817
CE2
TRP
C
56
59.512
90.266
−6.287
1.00
23.40
C


ATOM
3818
CE3
TRP
C
56
61.384
88.723
−6.383
1.00
25.94
C


ATOM
3819
CD1
TRP
C
56
60.374
91.988
−5.141
1.00
23.59
C


ATOM
3820
NE1
TRP
C
56
59.249
91.513
−5.771
1.00
25.69
C


ATOM
3821
CZ2
TRP
C
56
58.679
89.384
−7.003
1.00
24.25
C


ATOM
3822
CZ3
TRP
C
56
60.555
87.832
−7.095
1.00
26.08
C


ATOM
3823
CH2
TRP
C
56
59.209
88.177
−7.398
1.00
29.68
C


ATOM
3824
C
TRP
C
56
63.994
90.185
−2.504
1.00
22.58
C


ATOM
3825
O
TRP
C
56
64.379
91.119
−1.805
1.00
23.17
C


ATOM
3826
N
ASP
C
57
64.698
89.064
−2.660
1.00
24.44
C


ATOM
3827
CA
ASP
C
57
65.949
88.838
−1.931
1.00
26.43
C


ATOM
3828
CB
ASP
C
57
66.293
87.334
−1.894
1.00
26.66
C


ATOM
3829
CG
ASP
C
57
66.722
86.762
−3.256
1.00
31.79
C


ATOM
3830
OD1
ASP
C
57
67.168
85.599
−3.268
1.00
36.19
C


ATOM
3831
OD2
ASP
C
57
66.613
87.435
−4.298
1.00
30.66
C


ATOM
3832
C
ASP
C
57
67.173
89.647
−2.317
1.00
29.20
C


ATOM
3833
O
ASP
C
57
68.161
89.651
−1.589
1.00
32.18
C


ATOM
3834
N
LYS
C
58
67.084
90.363
−3.428
1.00
31.78
C


ATOM
3835
CA
LYS
C
58
68.180
91.182
−3.928
1.00
31.51
C


ATOM
3836
CB
LYS
C
58
69.081
90.366
−4.866
1.00
37.98
C


ATOM
3837
CG
LYS
C
58
70.061
89.452
−4.166
1.00
49.29
C


ATOM
3838
CD
LYS
C
58
69.648
87.987
−4.237
1.00
58.25
C


ATOM
3839
CE
LYS
C
58
69.569
87.484
−5.686
1.00
55.39
C


ATOM
3840
NZ
LYS
C
58
68.893
86.136
−5.803
1.00
46.73
C


ATOM
3841
C
LYS
C
58
67.614
92.311
−4.741
1.00
29.28
C


ATOM
3842
O
LYS
C
58
66.442
92.301
−5.106
1.00
29.16
C


ATOM
3843
N
PRO
C
59
68.429
93.340
−4.958
1.00
32.29
C


ATOM
3844
CD
PRO
C
59
69.835
93.582
−4.659
1.00
26.68
C


ATOM
3845
CA
PRO
C
59
67.912
94.387
−5.841
1.00
24.28
C


ATOM
3846
CB
PRO
C
59
68.919
95.530
−5.673
1.00
28.93
C


ATOM
3847
CG
PRO
C
59
70.198
94.856
−5.368
1.00
30.90
C


ATOM
3848
C
PRO
C
59
67.949
93.982
−7.321
1.00
29.24
C


ATOM
3849
O
PRO
C
59
68.694
93.012
−7.627
1.00
23.42
C


ATOM
3850
N
ALA
C
60
67.208
94.594
−8.172
1.00
28.57
C


ATOM
3851
CA
ALA
C
60
67.247
94.196
−9.570
1.00
31.26
C


ATOM
3852
CB
ALA
C
60
66.284
95.039
−10.363
1.00
24.31
C


ATOM
3853
C
ALA
C
60
68.674
94.376
−10.126
1.00
32.02
C


ATOM
3854
O
ALA
C
60
69.403
95.259
−9.700
1.00
29.35
C


ATOM
3855
N
PRO
C
61
69.101
93.498
−11.046
1.00
29.11
C


ATOM
3856
CD
PRO
C
61
68.453
92.245
−11.466
1.00
26.65
C


ATOM
3857
CA
PRO
C
61
70.449
93.643
−11.625
1.00
26.17
C


ATOM
3858
CB
PRO
C
61
70.690
92.296
−12.307
1.00
28.95
C


ATOM
3859
CG
PRO
C
61
69.280
91.838
−12.676
1.00
27.22
C


ATOM
3860
C
PRO
C
61
70.327
94.812
−12.632
1.00
26.90
C


ATOM
3861
O
PRO
C
61
69.221
95.303
−12.859
1.00
22.51
C


ATOM
3862
N
LYS
C
62
71.430
95.280
−13.208
1.00
21.80
C


ATOM
3863
CA
LYS
C
62
71.379
96.409
−14.158
1.00
24.26
C


ATOM
3864
CB
LYS
C
62
72.173
97.615
−13.637
1.00
26.24
C


ATOM
3865
CG
LYS
C
62
71.776
98.107
−12.246
1.00
39.00
C


ATOM
3866
CD
LYS
C
62
71.465
99.585
−12.222
1.00
41.85
C


ATOM
3867
CE
LYS
C
62
71.357
100.084
−10.800
1.00
46.78
C


ATOM
3868
NZ
LYS
C
62
72.692
100.117
−10.118
1.00
55.22
C


ATOM
3869
C
LYS
C
62
71.955
96.043
−15.507
1.00
21.61
C


ATOM
3870
O
LYS
C
62
72.735
95.097
−15.607
1.00
25.68
C


ATOM
3871
N
CYS
C
63
71.585
96.790
−16.549
1.00
27.02
C


ATOM
3872
CA
CYS
C
63
72.113
96.526
−17.903
1.00
27.02
C


ATOM
3873
C
CYS
C
63
73.118
97.601
−18.302
1.00
30.37
C


ATOM
3874
O
CYS
C
63
72.751
98.758
−18.416
1.00
32.00
C


ATOM
3875
CB
CYS
C
63
70.989
96.504
−18.932
1.00
28.91
C


ATOM
3876
SG
CYS
C
63
69.851
95.146
−18.602
1.00
29.20
C


ATOM
3877
N
GLU
C
64
74.376
97.213
−18.514
1.00
23.24
C


ATOM
3878
CA
GLU
C
64
75.417
98.167
−18.890
1.00
30.86
C


ATOM
3879
CB
GLU
C
64
76.707
97.873
−18.098
1.00
31.31
C


ATOM
3880
CG
GLU
C
64
77.231
99.028
−17.228
1.00
39.58
C


ATOM
3881
CD
GLU
C
64
78.383
98.616
−16.287
1.00
40.40
C


ATOM
3882
OE1
GLU
C
64
79.134
97.679
−16.620
1.00
26.79
C


ATOM
3883
OE2
GLU
C
64
78.543
99.238
−15.208
1.00
48.25
C


ATOM
3884
C
GLU
C
64
75.703
98.035
−20.380
1.00
25.87
C


ATOM
3885
O
GLU
C
64
75.849
96.923
−20.882
1.00
19.45
C


ATOM
3886
N
TYR
C
65
75.782
99.155
−21.093
1.00
25.32
C


ATOM
3887
CA
TYR
C
65
76.106
99.110
−22.530
1.00
31.90
C


ATOM
3888
CB
TYR
C
65
76.325
100.536
−23.061
1.00
36.00
C


ATOM
3889
CG
TYR
C
65
76.254
100.650
−24.570
1.00
42.53
C


ATOM
3890
CD1
TYR
C
65
75.041
100.470
−25.238
1.00
42.78
C


ATOM
3891
CE1
TYR
C
65
74.951
100.568
−26.621
1.00
40.68
C


ATOM
3892
CD2
TYR
C
65
77.396
100.937
−25.331
1.00
48.40
C


ATOM
3893
CE2
TYR
C
65
77.321
101.040
−26.729
1.00
48.66
C


ATOM
3894
CZ
TYR
C
65
76.086
100.848
−27.366
1.00
48.38
C


ATOM
3895
OH
TYR
C
65
75.998
100.897
−28.750
1.00
49.96
C


ATOM
3896
C
TYR
C
65
77.407
98.304
−22.704
1.00
26.60
C


ATOM
3897
O
TYR
C
65
78.410
98.634
−22.101
1.00
27.68
C


ATOM
3898
N
PHE
C
66
77.404
97.279
−23.541
1.00
24.63
C


ATOM
3899
CA
PHE
C
66
78.583
96.428
−23.736
1.00
25.64
C


ATOM
3900
CB
PHE
C
66
78.250
95.339
−24.776
1.00
30.86
C


ATOM
3901
CG
PHE
C
66
79.408
94.427
−25.130
1.00
30.23
C


ATOM
3902
CD1
PHE
C
66
80.137
93.775
−24.143
1.00
25.24
C


ATOM
3903
CD2
PHE
C
66
79.753
94.208
−26.481
1.00
33.99
C


ATOM
3904
CE1
PHE
C
66
81.206
92.899
−24.478
1.00
28.31
C


ATOM
3905
CE2
PHE
C
66
80.822
93.329
−26.838
1.00
29.10
C


ATOM
3906
CZ
PHE
C
66
81.550
92.672
−25.826
1.00
27.94
C


ATOM
3907
C
PHE
C
66
79.896
97.121
−24.133
1.00
32.19
C


ATOM
3908
O
PHE
C
66
79.931
97.955
−25.044
1.00
29.06
C


ATOM
3909
N
ASN
C
67
80.977
96.766
−23.440
1.00
25.19
C


ATOM
3910
CA
ASN
C
67
82.297
97.296
−23.754
1.00
27.44
C


ATOM
3911
CB
ASN
C
67
82.845
98.203
−22.639
1.00
28.12
C


ATOM
3912
CG
ASN
C
67
84.126
98.921
−23.066
1.00
33.33
C


ATOM
3913
OD1
ASN
C
67
84.904
98.390
−23.870
1.00
27.62
C


ATOM
3914
ND2
ASN
C
67
84.356
100.126
−22.528
1.00
28.36
C


ATOM
3915
C
ASN
C
67
83.245
96.106
−23.942
1.00
30.05
C


ATOM
3916
O
ASN
C
67
83.706
95.489
−22.967
1.00
18.34
C


ATOM
3917
N
LYS
C
68
83.565
95.816
−25.199
1.00
24.67
C


ATOM
3918
CA
LYS
C
68
84.428
94.686
−25.511
1.00
29.80
C


ATOM
3919
CB
LYS
C
68
84.491
94.441
−27.024
1.00
29.48
C


ATOM
3920
CG
LYS
C
68
85.095
95.574
−27.875
1.00
39.59
C


ATOM
3921
CD
LYS
C
68
85.233
95.076
−29.326
1.00
45.75
C


ATOM
3922
CE
LYS
C
68
85.993
96.016
−30.254
1.00
52.45
C


ATOM
3923
NZ
LYS
C
68
85.111
96.916
−31.073
1.00
56.09
C


ATOM
3924
C
LYS
C
68
85.821
94.831
−24.995
1.00
32.29
C


ATOM
3925
O
LYS
C
68
86.541
93.833
−24.921
1.00
27.75
C


ATOM
3926
N
TYR
C
69
86.237
96.059
−24.663
1.00
25.84
C


ATOM
3927
CA
TYR
C
69
87.599
96.229
−24.153
1.00
24.63
C


ATOM
3928
CB
TYR
C
69
88.147
97.625
−24.434
1.00
27.26
C


ATOM
3929
CG
TYR
C
69
88.161
97.948
−25.876
1.00
31.88
C


ATOM
3930
CD1
TYR
C
69
87.105
98.635
−26.441
1.00
25.30
C


ATOM
3931
CE1
TYR
C
69
87.067
98.885
−27.791
1.00
33.19
C


ATOM
3932
CD2
TYR
C
69
89.213
97.503
−26.708
1.00
32.15
C


ATOM
3933
CE2
TYR
C
69
89.186
97.749
−28.079
1.00
32.52
C


ATOM
3934
CZ
TYR
C
69
88.095
98.453
−28.614
1.00
34.98
C


ATOM
3935
OH
TYR
C
69
88.031
98.764
−29.952
1.00
32.72
C


ATOM
3936
C
TYR
C
69
87.687
95.997
−22.673
1.00
30.28
C


ATOM
3937
O
TYR
C
69
88.777
95.781
−22.158
1.00
28.03
C


ATOM
3938
N
SER
C
70
86.565
96.050
−21.967
1.00
29.29
C


ATOM
3939
CA
SER
C
70
86.597
95.868
−20.505
1.00
23.26
C


ATOM
3940
CB
SER
C
70
85.204
96.061
−19.871
1.00
25.19
C


ATOM
3941
OG
SER
C
70
84.518
97.144
−20.277
1.00
30.67
C


ATOM
3942
C
SER
C
70
87.175
94.504
−20.128
1.00
29.31
C


ATOM
3943
O
SER
C
70
86.714
93.455
−20.641
1.00
33.25
C


ATOM
3944
N
SER
C
71
88.113
94.596
−19.092
1.00
34.01
C


ATOM
3945
CA
SER
C
71
88.825
93.422
−18.570
1.00
33.07
C


ATOM
3946
CB
SER
C
71
90.139
93.197
−19.334
1.00
37.09
C


ATOM
3947
OG
SER
C
71
90.774
91.992
−18.919
1.00
38.97
C


ATOM
3948
C
SER
C
71
89.145
93.621
−17.099
1.00
31.37
C


ATOM
3949
O
SER
C
71
89.965
94.464
−16.748
1.00
31.95
C


ATOM
3950
N
CYS
C
72
88.518
92.836
−16.230
1.00
32.50
C


ATOM
3951
CA
CYS
C
72
88.760
92.984
−14.803
1.00
31.24
C


ATOM
3952
C
CYS
C
72
89.899
92.131
−14.294
1.00
32.97
C


ATOM
3953
O
CYS
C
72
90.062
90.989
−14.695
1.00
37.51
C


ATOM
3954
CB
CYS
C
72
87.479
92.670
−14.032
1.00
31.14
C


ATOM
3955
SG
CYS
C
72
86.104
93.792
−14.488
1.00
35.33
C


ATOM
3956
N
PRO
C
73
90.723
92.681
−13.401
1.00
31.28
C


ATOM
3957
CD
PRO
C
73
90.688
94.015
−12.785
1.00
34.25
C


ATOM
3958
CA
PRO
C
73
91.836
91.900
−12.875
1.00
37.83
C


ATOM
3959
CB
PRO
C
73
92.700
92.953
−12.199
1.00
39.00
C


ATOM
3960
CG
PRO
C
73
91.667
93.862
−11.635
1.00
36.58
C


ATOM
3961
C
PRO
C
73
91.325
90.889
−11.872
1.00
40.84
C


ATOM
3962
O
PRO
C
73
90.265
91.088
−11.284
1.00
40.57
C


ATOM
3963
N
GLU
C
74
92.185
89.834
−11.599
1.00
44.75
C


ATOM
3964
CA
GLU
C
74
91.750
88.788
−10.731
1.00
50.06
C


ATOM
3965
CB
GLU
C
74
92.854
87.743
−10.680
1.00
55.68
C


ATOM
3966
CG
GLU
C
74
93.109
86.845
−9.771
1.00
64.35
C


ATOM
3967
CD
GLU
C
74
92.171
85.710
−10.135
1.00
67.16
C


ATOM
3968
OE1
GLU
C
74
92.147
85.315
−11.320
1.00
67.98
C


ATOM
3969
OE2
GLU
C
74
91.459
85.216
−9.236
1.00
69.43
C


ATOM
3970
C
GLU
C
74
91.482
89.377
−9.347
1.00
45.69
C


ATOM
3971
O
GLU
C
74
92.282
90.168
−8.832
1.00
43.16
C


ATOM
3972
N
PRO
C
75
90.195
89.176
−8.898
1.00
45.27
C


ATOM
3973
CD
PRO
C
75
89.118
88.373
−9.504
1.00
46.99
C


ATOM
3974
CA
PRO
C
75
89.789
89.765
−7.622
1.00
46.84
C


ATOM
3975
CB
PRO
C
75
88.278
89.575
−7.643
1.00
45.59
C


ATOM
3976
CG
PRO
C
75
88.129
88.251
−8.380
1.00
43.10
C


ATOM
3977
C
PRO
C
75
90.484
88.987
−6.471
1.00
46.31
C


ATOM
3978
O
PRO
C
75
90.622
87.764
−6.533
1.00
43.58
C


ATOM
3979
N
ILE
C
76
90.967
89.682
−5.452
1.00
44.06
C


ATOM
3980
CA
ILE
C
76
91.585
88.961
−4.346
1.00
49.43
C


ATOM
3981
CB
ILE
C
76
93.091
89.252
−4.220
1.00
50.66
C


ATOM
3982
CG2
ILE
C
76
93.648
88.589
−2.960
1.00
51.02
C


ATOM
3983
CG1
ILE
C
76
93.829
88.692
−5.433
1.00
53.97
C


ATOM
3984
CD1
ILE
C
76
95.327
88.938
−5.393
1.00
58.58
C


ATOM
3985
C
ILE
C
76
90.910
89.320
−3.041
1.00
47.39
C


ATOM
3986
O
ILE
C
76
90.663
90.496
−2.770
1.00
45.77
C


ATOM
3987
N
VAL
C
77
90.596
88.303
−2.240
1.00
47.49
C


ATOM
3988
CA
VAL
C
77
89.933
88.516
−0.949
1.00
47.30
C


ATOM
3989
CB
VAL
C
77
88.466
87.930
−0.945
1.00
46.81
C


ATOM
3990
CG1
VAL
C
77
87.848
88.039
0.474
1.00
43.39
C


ATOM
3991
CG2
VAL
C
77
87.604
88.654
−1.986
1.00
42.04
C


ATOM
3992
C
VAL
C
77
90.725
87.855
0.188
1.00
47.58
C


ATOM
3993
O
VAL
C
77
90.823
86.620
0.263
1.00
45.09
C


ATOM
3994
N
PRO
C
78
91.301
88.669
1.086
1.00
44.97
C


ATOM
3995
CD
PRO
C
78
91.365
90.140
1.032
1.00
45.60
C


ATOM
3996
CA
PRO
C
78
92.078
88.143
2.221
1.00
44.08
C


ATOM
3997
CB
PRO
C
78
92.461
89.407
2.989
1.00
45.76
C


ATOM
3998
CG
PRO
C
78
92.593
90.447
1.892
1.00
46.75
C


ATOM
3999
C
PRO
C
78
91.208
87.217
3.065
1.00
43.84
C


ATOM
4000
O
PRO
C
78
90.088
87.568
3.403
1.00
43.30
C


ATOM
4001
N
GLY
C
79
91.705
86.035
3.395
1.00
44.86
C


ATOM
4002
CA
GLY
C
79
90.913
85.126
4.204
1.00
43.45
C


ATOM
4003
C
GLY
C
79
89.868
84.402
3.379
1.00
45.18
C


ATOM
4004
O
GLY
C
79
89.025
83.667
3.914
1.00
43.18
C


ATOM
4005
N
GLY
C
80
89.922
84.605
2.067
1.00
41.25
C


ATOM
4006
CA
GLY
C
80
88.970
83.946
1.185
1.00
39.64
C


ATOM
4007
C
GLY
C
80
89.622
83.287
−0.022
1.00
44.23
C


ATOM
4008
O
GLY
C
80
90.827
83.420
−0.269
1.00
40.56
C


ATOM
4009
N
TYR
C
81
88.829
82.545
−0.779
1.00
42.62
C


ATOM
4010
CA
TYR
C
81
89.343
81.905
−1.973
1.00
46.24
C


ATOM
4011
CB
TYR
C
81
89.908
80.496
−1.655
1.00
44.92
C


ATOM
4012
CG
TYR
C
81
88.934
79.522
−1.020
1.00
46.64
C


ATOM
4013
CD1
TYR
C
81
88.405
78.463
−1.754
1.00
48.03
C


ATOM
4014
CE1
TYR
C
81
87.438
77.618
−1.221
1.00
48.43
C


ATOM
4015
CD2
TYR
C
81
88.478
79.709
0.281
1.00
47.96
C


ATOM
4016
CE2
TYR
C
81
87.503
78.871
0.826
1.00
50.43
C


ATOM
4017
CZ
TYR
C
81
86.980
77.827
0.062
1.00
51.08
C


ATOM
4018
OH
TYR
C
81
85.948
77.038
0.550
1.00
54.25
C


ATOM
4019
C
TYR
C
81
88.233
81.854
−3.028
1.00
50.13
C


ATOM
4020
O
TYR
C
81
87.039
81.840
−2.711
1.00
46.05
C


ATOM
4021
N
LYS
C
82
88.643
81.853
−4.289
1.00
50.32
C


ATOM
4022
CA
LYS
C
82
87.697
81.828
−5.380
1.00
53.74
C


ATOM
4023
CB
LYS
C
82
88.393
82.238
−6.681
1.00
53.51
C


ATOM
4024
CG
LYS
C
82
87.466
82.280
−7.884
1.00
54.66
C


ATOM
4025
CD
LYS
C
82
88.182
82.779
−9.126
1.00
52.50
C


ATOM
4026
CE
LYS
C
82
89.254
81.803
−9.563
1.00
53.91
C


ATOM
4027
NZ
LYS
C
82
90.030
82.321
−10.718
1.00
52.48
C


ATOM
4028
C
LYS
C
82
87.089
80.447
−5.528
1.00
54.39
C


ATOM
4029
O
LYS
C
82
87.782
79.435
−5.429
1.00
54.15
C


ATOM
4030
N
ILE
C
83
85.783
80.412
−5.740
1.00
53.36
C


ATOM
4031
CA
ILE
C
83
85.083
79.157
−5.935
1.00
56.28
C


ATOM
4032
CB
ILE
C
83
83.815
79.085
−5.070
1.00
57.52
C


ATOM
4033
CG2
ILE
C
83
82.803
78.186
−5.698
1.00
58.38
C


ATOM
4034
CG1
ILE
C
83
84.162
78.510
−3.702
1.00
59.41
C


ATOM
4035
CD1
ILE
C
83
85.312
79.203
−3.034
1.00
64.54
C


ATOM
4036
C
ILE
C
83
84.698
79.018
−7.408
1.00
60.57
C


ATOM
4037
O
ILE
C
83
84.790
77.926
−7.969
1.00
61.13
C


ATOM
4038
N
ARG
C
84
84.274
80.125
−8.025
1.00
58.19
C


ATOM
4039
CA
ARG
C
84
83.859
80.134
−9.432
1.00
55.53
C


ATOM
4040
CB
ARG
C
84
82.340
80.259
−9.557
1.00
59.40
C


ATOM
4041
CG
ARG
C
84
81.555
79.123
−8.937
1.00
66.78
C


ATOM
4042
CD
ARG
C
84
80.080
79.471
−8.783
1.00
70.71
C


ATOM
4043
NE
ARG
C
84
79.338
78.353
−8.204
1.00
78.82
C


ATOM
4044
CZ
ARG
C
84
78.041
78.384
−7.902
1.00
82.83
C


ATOM
4045
NH1
ARG
C
84
77.331
79.487
−8.124
1.00
84.17
C


ATOM
4046
NH2
ARG
C
84
77.454
77.308
−7.378
1.00
84.81
C


ATOM
4047
C
ARG
C
84
84.475
81.305
−10.156
1.00
54.25
C


ATOM
4048
O
ARG
C
84
84.812
82.313
−9.543
1.00
52.78
C


ATOM
4049
N
GLY
C
85
84.612
81.164
−11.468
1.00
51.61
C


ATOM
4050
CA
GLY
C
85
85.166
82.234
−12.265
1.00
52.64
C


ATOM
4051
C
GLY
C
85
86.542
81.935
−12.801
1.00
52.39
C


ATOM
4052
O
GLY
C
85
87.400
81.412
−12.093
1.00
53.26
C


ATOM
4053
N
SER
C
86
86.768
82.264
−14.063
1.00
56.65
C


ATOM
4054
CA
SER
C
86
88.074
82.013
−14.636
1.00
60.26
C


ATOM
4055
CB
SER
C
86
88.078
80.685
−15.393
1.00
63.40
C


ATOM
4056
OG
SER
C
86
89.410
80.222
−15.570
1.00
73.69
C


ATOM
4057
C
SER
C
86
88.482
83.126
−15.567
1.00
56.75
C


ATOM
4058
O
SER
C
86
87.650
83.698
−16.257
1.00
55.15
C


ATOM
4059
N
THR
C
87
89.775
83.428
−15.565
1.00
58.49
C


ATOM
4060
CA
THR
C
87
90.343
84.457
−16.417
1.00
57.84
C


ATOM
4061
CB
THR
C
87
91.816
84.711
−16.006
1.00
62.33
C


ATOM
4062
OG1
THR
C
87
91.873
85.892
−15.197
1.00
69.91
C


ATOM
4063
CG2
THR
C
87
92.724
84.863
−17.215
1.00
64.96
C


ATOM
4064
C
THR
C
87
90.257
84.034
−17.886
1.00
55.75
C


ATOM
4065
O
THR
C
87
90.241
82.842
−18.198
1.00
56.65
C


ATOM
4066
N
PRO
C
88
90.134
85.007
−18.805
1.00
52.96
C


ATOM
4067
CD
PRO
C
88
90.192
84.766
−20.259
1.00
53.18
C


ATOM
4068
CA
PRO
C
88
90.069
86.442
−18.533
1.00
52.06
C


ATOM
4069
CB
PRO
C
88
90.533
87.060
−19.849
1.00
50.34
C


ATOM
4070
CG
PRO
C
88
89.909
86.162
−20.842
1.00
52.45
C


ATOM
4071
C
PRO
C
88
88.630
86.844
−18.183
1.00
47.83
C


ATOM
4072
O
PRO
C
88
87.666
86.219
−18.628
1.00
43.02
C


ATOM
4073
N
TYR
C
89
88.502
87.903
−17.398
1.00
40.94
C


ATOM
4074
CA
TYR
C
89
87.204
88.376
−16.977
1.00
40.87
C


ATOM
4075
CB
TYR
C
89
87.276
88.839
−15.516
1.00
41.58
C


ATOM
4076
CG
TYR
C
89
87.666
87.730
−14.570
1.00
47.21
C


ATOM
4077
CD1
TYR
C
89
88.886
87.759
−13.879
1.00
48.57
C


ATOM
4078
CE1
TYR
C
89
89.260
86.711
−13.043
1.00
52.12
C


ATOM
4079
CD2
TYR
C
89
86.835
86.625
−14.397
1.00
47.83
C


ATOM
4080
CE2
TYR
C
89
87.203
85.573
−13.567
1.00
52.56
C


ATOM
4081
CZ
TYR
C
89
88.409
85.618
−12.892
1.00
52.86
C


ATOM
4082
OH
TYR
C
89
88.734
84.568
−12.059
1.00
55.18
C


ATOM
4083
C
TYR
C
89
86.775
89.516
−17.874
1.00
37.75
C


ATOM
4084
O
TYR
C
89
87.368
90.582
−17.837
1.00
35.86
C


ATOM
4085
N
ARG
C
90
85.742
89.282
−18.677
1.00
36.76
C


ATOM
4086
CA
ARG
C
90
85.227
90.291
−19.591
1.00
31.74
C


ATOM
4087
CB
ARG
C
90
84.956
89.672
−20.957
1.00
39.30
C


ATOM
4088
CG
ARG
C
90
86.211
89.526
−21.808
1.00
51.87
C


ATOM
4089
CD
ARG
C
90
86.620
88.085
−21.981
1.00
60.63
C


ATOM
4090
NE
ARG
C
90
87.619
87.933
−23.048
1.00
68.24
C


ATOM
4091
CZ
ARG
C
90
88.091
86.767
−23.501
1.00
68.54
C


ATOM
4092
NH1
ARG
C
90
87.673
85.604
−22.993
1.00
64.59
C


ATOM
4093
NH2
ARG
C
90
88.987
86.767
−24.479
1.00
70.32
C


ATOM
4094
C
ARG
C
90
83.959
90.915
−19.048
1.00
31.15
C


ATOM
4095
O
ARG
C
90
83.414
90.456
−18.040
1.00
24.92
C


ATOM
4096
N
HIS
C
91
83.483
91.955
−19.725
1.00
23.82
C


ATOM
4097
CA
HIS
C
91
82.285
92.674
−19.301
1.00
23.05
C


ATOM
4098
CB
HIS
C
91
81.872
93.640
−20.406
1.00
26.29
C


ATOM
4099
CG
HIS
C
91
80.849
94.665
−19.998
1.00
23.49
C


ATOM
4100
CD2
HIS
C
91
80.142
95.553
−20.744
1.00
20.45
C


ATOM
4101
ND1
HIS
C
91
80.515
94.920
−18.685
1.00
28.99
C


ATOM
4102
CE1
HIS
C
91
79.651
95.923
−18.637
1.00
19.30
C


ATOM
4103
NE2
HIS
C
91
79.412
96.325
−19.873
1.00
28.70
C


ATOM
4104
C
HIS
C
91
81.142
91.717
−19.003
1.00
24.93
C


ATOM
4105
O
HIS
C
91
80.771
90.926
−19.862
1.00
22.92
C


ATOM
4106
N
GLY
C
92
80.575
91.808
−17.801
1.00
25.08
C


ATOM
4107
CA
GLY
C
92
79.459
90.949
−17.435
1.00
25.83
C


ATOM
4108
C
GLY
C
92
79.834
89.612
−16.803
1.00
26.66
C


ATOM
4109
O
GLY
C
92
78.964
88.947
−16.233
1.00
30.96
C


ATOM
4110
N
ASP
C
93
81.098
89.203
−16.913
1.00
24.69
C


ATOM
4111
CA
ASP
C
93
81.558
87.940
−16.311
1.00
28.03
C


ATOM
4112
CB
ASP
C
93
83.001
87.609
−16.749
1.00
26.26
C


ATOM
4113
CG
ASP
C
93
83.101
87.123
−18.214
1.00
33.28
C


ATOM
4114
OD1
ASP
C
93
84.243
86.973
−18.725
1.00
35.30
C


ATOM
4115
OD2
ASP
C
93
82.056
86.885
−18.859
1.00
33.91
C


ATOM
4116
C
ASP
C
93
81.535
88.080
−14.791
1.00
32.16
C


ATOM
4117
O
ASP
C
93
81.641
89.197
−14.254
1.00
32.11
C


ATOM
4118
N
SER
C
94
81.400
86.966
−14.082
1.00
27.90
C


ATOM
4119
CA
SER
C
94
81.395
87.050
−12.632
1.00
37.96
C


ATOM
4120
CB
SER
C
94
79.983
86.889
−12.104
1.00
35.76
C


ATOM
4121
OG
SER
C
94
79.577
85.556
−12.314
1.00
39.23
C


ATOM
4122
C
SER
C
94
82.287
85.986
−11.997
1.00
38.51
C


ATOM
4123
O
SER
C
94
82.713
85.038
−12.655
1.00
36.22
C


ATOM
4124
N
VAL
C
95
82.550
86.154
−10.706
1.00
38.03
C


ATOM
4125
CA
VAL
C
95
83.373
85.221
−9.943
1.00
42.81
C


ATOM
4126
CB
VAL
C
95
84.825
85.728
−9.743
1.00
41.37
C


ATOM
4127
CG1
VAL
C
95
85.454
86.006
−11.073
1.00
46.63
C


ATOM
4128
CG2
VAL
C
95
84.840
86.980
−8.916
1.00
36.92
C


ATOM
4129
C
VAL
C
95
82.724
85.111
−8.587
1.00
43.66
C


ATOM
4130
O
VAL
C
95
82.023
86.024
−8.155
1.00
43.47
C


ATOM
4131
N
THR
C
96
82.939
83.988
−7.918
1.00
45.64
C


ATOM
4132
CA
THR
C
96
82.356
83.806
−6.599
1.00
46.87
C


ATOM
4133
CB
THR
C
96
81.258
82.736
−6.600
1.00
46.87
C


ATOM
4134
OG1
THR
C
96
80.233
83.101
−7.531
1.00
40.12
C


ATOM
4135
CG2
THR
C
96
80.635
82.635
−5.208
1.00
50.66
C


ATOM
4136
C
THR
C
96
83.432
83.403
−5.612
1.00
43.20
C


ATOM
4137
O
THR
C
96
84.363
82.686
−5.962
1.00
41.73
C


ATOM
4138
N
PHE
C
97
83.302
83.886
−4.387
1.00
39.07
C


ATOM
4139
CA
PHE
C
97
84.267
83.578
−3.349
1.00
43.95
C


ATOM
4140
CB
PHE
C
97
84.898
84.854
−2.810
1.00
45.41
C


ATOM
4141
CG
PHE
C
97
85.850
85.519
−3.750
1.00
46.14
C


ATOM
4142
CD1
PHE
C
97
85.422
86.543
−4.594
1.00
42.85
C


ATOM
4143
CD2
PHE
C
97
87.195
85.162
−3.749
1.00
45.67
C


ATOM
4144
CE1
PHE
C
97
86.320
87.194
−5.406
1.00
39.79
C


ATOM
4145
CE2
PHE
C
97
88.101
85.812
−4.562
1.00
42.72
C


ATOM
4146
CZ
PHE
C
97
87.663
86.829
−5.390
1.00
41.65
C


ATOM
4147
C
PHE
C
97
83.646
82.859
−2.158
1.00
46.38
C


ATOM
4148
O
PHE
C
97
82.421
82.804
−1.988
1.00
39.27
C


ATOM
4149
N
ALA
C
98
84.530
82.332
−1.318
1.00
48.32
C


ATOM
4150
CA
ALA
C
98
84.155
81.664
−0.076
1.00
46.94
C


ATOM
4151
CB
ALA
C
98
84.112
80.158
−0.277
1.00
46.22
C


ATOM
4152
C
ALA
C
98
85.254
82.038
0.929
1.00
46.65
C


ATOM
4153
O
ALA
C
98
86.394
82.293
0.536
1.00
44.10
C


ATOM
4154
N
CYS
C
99
84.914
82.103
2.212
1.00
46.08
C


ATOM
4155
CA
CYS
C
99
85.917
82.423
3.209
1.00
47.08
C


ATOM
4156
C
CYS
C
99
86.590
81.156
3.711
1.00
46.05
C


ATOM
4157
O
CYS
C
99
85.963
80.096
3.760
1.00
43.34
C


ATOM
4158
CB
CYS
C
99
85.289
83.168
4.375
1.00
44.52
C


ATOM
4159
SG
CYS
C
99
84.727
84.800
3.844
1.00
44.83
C


ATOM
4160
N
LYS
C
100
87.874
81.273
4.056
1.00
47.36
C


ATOM
4161
CA
LYS
C
100
88.645
80.147
4.574
1.00
48.81
C


ATOM
4162
CB
LYS
C
100
90.135
80.489
4.594
1.00
49.39
C


ATOM
4163
CG
LYS
C
100
90.750
80.658
3.219
1.00
54.21
C


ATOM
4164
CD
LYS
C
100
92.190
81.114
3.317
1.00
54.87
C


ATOM
4165
CE
LYS
C
100
92.806
81.267
1.942
1.00
57.13
C


ATOM
4166
NZ
LYS
C
100
94.197
81.768
2.053
1.00
60.72
C


ATOM
4167
C
LYS
C
100
88.165
79.861
5.997
1.00
49.95
C


ATOM
4168
O
LYS
C
100
87.495
80.705
6.619
1.00
48.69
C


ATOM
4169
N
THR
C
101
88.786
78.765
6.373
1.00
51.22
C


ATOM
4170
CA
THR
C
101
88.580
78.229
7.709
1.00
55.29
C


ATOM
4171
CB
THR
C
101
89.528
77.069
8.012
1.00
57.10
C


ATOM
4172
OG1
THR
C
101
89.306
76.008
7.077
1.00
64.35
C


ATOM
4173
CG2
THR
C
101
89.308
76.559
9.429
1.00
57.34
C


ATOM
4174
C
THR
C
101
88.780
79.304
8.764
1.00
49.93
C


ATOM
4175
O
THR
C
101
89.894
79.947
8.793
1.00
47.27
C


ATOM
4176
N
ASN
C
102
88.004
79.652
9.763
1.00
46.86
C


ATOM
4177
CA
ASN
C
102
88.149
80.670
10.780
1.00
48.18
C


ATOM
4178
CB
ASN
C
102
89.557
80.629
11.363
1.00
46.95
C


ATOM
4179
CG
ASN
C
102
89.845
79.289
12.014
1.00
48.40
C


ATOM
4180
OD1
ASN
C
102
88.985
78.720
12.693
1.00
41.35
C


ATOM
4181
ND2
ASN
C
102
91.051
78.775
11.810
1.00
49.40
C


ATOM
4182
C
ASN
C
102
87.761
82.030
10.236
1.00
49.20
C


ATOM
4183
O
ASN
C
102
88.252
83.052
10.922
1.00
46.49
C


ATOM
4184
N
PHE
C
103
87.220
82.295
9.176
1.00
48.20
C


ATOM
4185
CA
PHE
C
103
86.584
83.570
8.755
1.00
49.30
C


ATOM
4186
CB
PHE
C
103
87.390
84.074
7.554
1.00
46.94
C


ATOM
4187
CG
PHE
C
103
88.859
84.187
7.814
1.00
45.01
C


ATOM
4188
CD1
PHE
C
103
89.671
83.059
7.792
1.00
48.38
C


ATOM
4189
CD2
PHE
C
103
89.437
85.421
8.082
1.00
48.98
C


ATOM
4190
CE1
PHE
C
103
91.045
83.157
8.034
1.00
48.94
C


ATOM
4191
CE2
PHE
C
103
90.810
85.533
8.328
1.00
48.39
C


ATOM
4192
CZ
PHE
C
103
91.616
84.400
8.302
1.00
46.82
C


ATOM
4193
C
PHE
C
103
85.097
83.521
8.378
1.00
50.21
C


ATOM
4194
O
PHE
C
103
84.570
82.478
7.968
1.00
49.82
C


ATOM
4195
N
SER
C
104
84.414
84.647
8.565
1.00
48.66
C


ATOM
4196
CA
SER
C
104
83.012
84.745
8.214
1.00
45.33
C


ATOM
4197
CB
SER
C
104
82.165
85.191
9.417
1.00
46.37
C


ATOM
4198
OG
SER
C
104
82.048
86.605
9.522
1.00
57.43
C


ATOM
4199
C
SER
C
104
82.984
85.792
7.108
1.00
41.51
C


ATOM
4200
O
SER
C
104
83.757
86.736
7.130
1.00
38.53
C


ATOM
4201
N
MET
C
105
82.114
85.616
6.129
1.00
41.72
C


ATOM
4202
CA
MET
C
105
82.036
86.573
5.038
1.00
43.60
C


ATOM
4203
CB
MET
C
105
81.601
85.872
3.748
1.00
44.67
C


ATOM
4204
CG
MET
C
105
81.330
86.830
2.579
1.00
40.80
C


ATOM
4205
SD
MET
C
105
80.914
85.922
1.118
1.00
45.07
C


ATOM
4206
CE
MET
C
105
82.489
85.186
0.675
1.00
39.55
C


ATOM
4207
C
MET
C
105
81.077
87.722
5.308
1.00
46.26
C


ATOM
4208
O
MET
C
105
79.972
87.519
5.816
1.00
44.17
C


ATOM
4209
N
ASN
C
106
81.518
88.927
4.963
1.00
47.38
C


ATOM
4210
CA
ASN
C
106
80.707
90.137
5.078
1.00
49.51
C


ATOM
4211
CB
ASN
C
106
81.391
91.160
5.984
1.00
56.07
C


ATOM
4212
CG
ASN
C
106
80.769
92.539
5.879
1.00
63.53
C


ATOM
4213
OD1
ASN
C
106
80.925
93.233
4.867
1.00
66.72
C


ATOM
4214
ND2
ASN
C
106
80.048
92.943
6.921
1.00
68.89
C


ATOM
4215
C
ASN
C
106
80.572
90.688
3.645
1.00
49.28
C


ATOM
4216
O
ASN
C
106
81.566
91.073
3.015
1.00
46.49
C


ATOM
4217
N
GLY
C
107
79.344
90.700
3.130
1.00
50.96
C


ATOM
4218
CA
GLY
C
107
79.102
91.178
1.776
1.00
47.29
C


ATOM
4219
C
GLY
C
107
78.620
90.074
0.846
1.00
46.96
C


ATOM
4220
O
GLY
C
107
78.421
88.940
1.275
1.00
48.97
C


ATOM
4221
N
ASN
C
108
78.439
90.398
−0.434
1.00
50.83
C


ATOM
4222
CA
ASN
C
108
77.974
89.429
−1.426
1.00
49.00
C


ATOM
4223
CB
ASN
C
108
77.392
90.148
−2.635
1.00
56.26
C


ATOM
4224
CG
ASN
C
108
76.392
91.180
−2.247
1.00
61.63
C


ATOM
4225
OD1
ASN
C
108
76.703
92.371
−2.168
1.00
67.56
C


ATOM
4226
ND2
ASN
C
108
75.176
90.735
−1.977
1.00
62.48
C


ATOM
4227
C
ASN
C
108
79.125
88.561
−1.887
1.00
46.12
C


ATOM
4228
O
ASN
C
108
80.217
89.064
−2.155
1.00
42.01
C


ATOM
4229
N
LYS
C
109
78.858
87.266
−2.016
1.00
38.69
C


ATOM
4230
CA
LYS
C
109
79.874
86.310
−2.407
1.00
41.12
C


ATOM
4231
CB
LYS
C
109
79.444
84.897
−2.023
1.00
44.04
C


ATOM
4232
CG
LYS
C
109
78.255
84.360
−2.804
1.00
45.47
C


ATOM
4233
CD
LYS
C
109
77.585
83.222
−2.019
1.00
51.74
C


ATOM
4234
CE
LYS
C
109
76.584
82.453
−2.865
1.00
59.58
C


ATOM
4235
NZ
LYS
C
109
77.262
81.664
−3.956
1.00
64.18
C


ATOM
4236
C
LYS
C
109
80.199
86.324
−3.874
1.00
40.75
C


ATOM
4237
O
LYS
C
109
81.189
85.714
−4.307
1.00
41.19
C


ATOM
4238
N
SER
C
110
79.358
86.988
−4.655
1.00
38.36
C


ATOM
4239
CA
SER
C
110
79.578
87.024
−6.101
1.00
36.23
C


ATOM
4240
CB
SER
C
110
78.437
86.305
−6.834
1.00
39.46
C


ATOM
4241
OG
SER
C
110
78.481
84.918
−6.579
1.00
36.52
C


ATOM
4242
C
SER
C
110
79.635
88.421
−6.581
1.00
29.42
C


ATOM
4243
O
SER
C
110
78.876
89.247
−6.098
1.00
28.55
C


ATOM
4244
N
VAL
C
111
80.560
88.692
−7.499
1.00
30.69
C


ATOM
4245
CA
VAL
C
111
80.678
90.017
−8.105
1.00
29.50
C


ATOM
4246
CB
VAL
C
111
81.934
90.782
−7.652
1.00
33.90
C


ATOM
4247
CG1
VAL
C
111
81.666
91.463
−6.325
1.00
32.11
C


ATOM
4248
CG2
VAL
C
111
83.135
89.825
−7.579
1.00
26.88
C


ATOM
4249
C
VAL
C
111
80.718
89.916
−9.632
1.00
29.99
C


ATOM
4250
O
VAL
C
111
80.996
88.853
−10.194
1.00
27.74
C


ATOM
4251
N
TRP
C
112
80.448
91.034
−10.299
1.00
25.72
C


ATOM
4252
CA
TRP
C
112
80.437
91.062
−11.756
1.00
25.20
C


ATOM
4253
CB
TRP
C
112
79.020
91.411
−12.237
1.00
26.69
C


ATOM
4254
CG
TRP
C
112
78.107
90.226
−12.146
1.00
33.34
C


ATOM
4255
CD2
TRP
C
112
77.481
89.706
−10.957
1.00
30.54
C


ATOM
4256
CE2
TRP
C
112
76.820
88.505
−11.322
1.00
33.04
C


ATOM
4257
CE3
TRP
C
112
77.421
90.133
−9.622
1.00
31.04
C


ATOM
4258
CD1
TRP
C
112
77.799
89.338
−13.158
1.00
37.78
C


ATOM
4259
NE1
TRP
C
112
77.029
88.309
−12.665
1.00
35.55
C


ATOM
4260
CZ2
TRP
C
112
76.110
87.729
−10.397
1.00
27.67
C


ATOM
4261
CZ3
TRP
C
112
76.713
89.357
−8.696
1.00
30.48
C


ATOM
4262
CH2
TRP
C
112
76.067
88.168
−9.092
1.00
26.73
C


ATOM
4263
C
TRP
C
112
81.456
92.052
−12.307
1.00
27.63
C


ATOM
4264
O
TRP
C
112
81.719
93.091
−11.701
1.00
22.47
C


ATOM
4265
N
CYS
C
113
82.042
91.719
−13.455
1.00
26.10
C


ATOM
4266
CA
CYS
C
113
83.025
92.593
−14.083
1.00
25.33
C


ATOM
4267
C
CYS
C
113
82.313
93.725
−14.830
1.00
30.04
C


ATOM
4268
O
CYS
C
113
81.611
93.488
−15.824
1.00
28.98
C


ATOM
4269
CB
CYS
C
113
83.897
91.780
−15.029
1.00
26.64
C


ATOM
4270
SG
CYS
C
113
85.096
92.793
−15.958
1.00
29.69
C


ATOM
4271
N
GLN
C
114
82.478
94.951
−14.336
1.00
22.99
C


ATOM
4272
CA
GLN
C
114
81.842
96.127
−14.927
1.00
25.16
C


ATOM
4273
CB
GLN
C
114
81.597
97.177
−13.835
1.00
28.73
C


ATOM
4274
CG
GLN
C
114
80.857
96.602
−12.630
1.00
36.89
C


ATOM
4275
CD
GLN
C
114
80.727
97.569
−11.457
1.00
42.08
C


ATOM
4276
OE1
GLN
C
114
79.802
98.365
−11.382
1.00
49.79
C


ATOM
4277
NE2
GLN
C
114
81.665
97.493
−10.539
1.00
49.11
C


ATOM
4278
C
GLN
C
114
82.630
96.739
−16.092
1.00
31.28
C


ATOM
4279
O
GLN
C
114
83.827
96.444
−16.284
1.00
27.34
C


ATOM
4280
N
ALA
C
115
81.956
97.594
−16.865
1.00
25.85
C


ATOM
4281
CA
ALA
C
115
82.564
98.246
−18.031
1.00
28.40
C


ATOM
4282
CB
ALA
C
115
81.577
99.211
−18.667
1.00
20.65
C


ATOM
4283
C
ALA
C
115
83.844
98.990
−17.651
1.00
28.38
C


ATOM
4284
O
ALA
C
115
84.797
99.004
−18.432
1.00
23.27
C


ATOM
4285
N
ASN
C
116
83.873
99.587
−16.456
1.00
23.81
C


ATOM
4286
CA
ASN
C
116
85.060
100.327
−16.002
1.00
28.02
C


ATOM
4287
CB
ASN
C
116
84.663
101.425
−14.993
1.00
28.32
C


ATOM
4288
CG
ASN
C
116
83.983
100.858
−13.746
1.00
27.71
C


ATOM
4289
OD1
ASN
C
116
84.129
99.674
−13.436
1.00
24.66
C


ATOM
4290
ND2
ASN
C
116
83.247
101.707
−13.020
1.00
23.76
C


ATOM
4291
C
ASN
C
116
86.139
99.447
−15.378
1.00
27.65
C


ATOM
4292
O
ASN
C
116
87.027
99.957
−14.695
1.00
31.18
C


ATOM
4293
N
ASN
C
117
86.060
98.132
−15.593
1.00
27.61
C


ATOM
4294
CA
ASN
C
117
87.030
97.189
−15.048
1.00
29.18
C


ATOM
4295
CB
ASN
C
117
88.475
97.578
−15.449
1.00
27.03
C


ATOM
4296
CG
ASN
C
117
88.724
97.389
−16.951
1.00
30.01
C


ATOM
4297
OD1
ASN
C
117
87.901
96.793
−17.638
1.00
27.30
C


ATOM
4298
ND2
ASN
C
117
89.853
97.883
−17.455
1.00
29.00
C


ATOM
4299
C
ASN
C
117
86.958
96.986
−13.543
1.00
31.56
C


ATOM
4300
O
ASN
C
117
87.748
96.230
−12.994
1.00
31.15
C


ATOM
4301
N
MET
C
118
86.035
97.652
−12.860
1.00
33.87
C


ATOM
4302
CA
MET
C
118
85.918
97.430
−11.421
1.00
38.31
C


ATOM
4303
CB
MET
C
118
85.431
98.700
−10.723
1.00
43.16
C


ATOM
4304
CG
MET
C
118
86.446
99.857
−10.744
1.00
53.52
C


ATOM
4305
SD
MET
C
118
85.763
101.385
−10.037
1.00
58.36
C


ATOM
4306
CE
MET
C
118
86.038
101.027
−8.271
1.00
63.17
C


ATOM
4307
C
MET
C
118
84.937
96.281
−11.182
1.00
41.22
C


ATOM
4308
O
MET
C
118
84.034
96.039
−12.001
1.00
39.23
C


ATOM
4309
N
TRP
C
119
85.108
95.588
−10.058
1.00
39.36
C


ATOM
4310
CA
TRP
C
119
84.276
94.458
−9.706
1.00
34.52
C


ATOM
4311
CB
TRP
C
119
85.044
93.494
−8.830
1.00
37.83
C


ATOM
4312
CG
TRP
C
119
85.916
92.608
−9.625
1.00
34.11
C


ATOM
4313
CD2
TRP
C
119
85.497
91.515
−10.449
1.00
33.34
C


ATOM
4314
CE2
TRP
C
119
86.652
90.975
−11.053
1.00
32.21
C


ATOM
4315
CE3
TRP
C
119
84.250
90.935
−10.741
1.00
29.94
C


ATOM
4316
CD1
TRP
C
119
87.264
92.691
−9.755
1.00
31.13
C


ATOM
4317
NE1
TRP
C
119
87.721
91.715
−10.608
1.00
36.90
C


ATOM
4318
CZ2
TRP
C
119
86.610
89.897
−11.919
1.00
29.11
C


ATOM
4319
CZ3
TRP
C
119
84.206
89.869
−11.594
1.00
31.88
C


ATOM
4320
CH2
TRP
C
119
85.384
89.352
−12.181
1.00
32.92
C


ATOM
4321
C
TRP
C
119
82.930
94.725
−9.066
1.00
45.62
C


ATOM
4322
O
TRP
C
119
82.742
95.703
−8.345
1.00
44.53
C


ATOM
4323
N
GLY
C
120
82.022
93.799
−9.399
1.00
52.00
C


ATOM
4324
CA
GLY
C
120
80.621
93.709
−8.986
1.00
49.90
C


ATOM
4325
C
GLY
C
120
79.864
94.960
−8.669
1.00
50.22
C


ATOM
4326
O
GLY
C
120
80.454
96.037
−8.703
1.00
53.01
C


ATOM
4327
N
PRO
C
121
78.546
94.853
−8.388
1.00
42.28
C


ATOM
4328
CD
PRO
C
121
77.760
93.635
−8.569
1.00
46.84
C


ATOM
4329
CA
PRO
C
121
77.673
95.988
−8.042
1.00
46.16
C


ATOM
4330
CB
PRO
C
121
76.263
95.395
−8.040
1.00
42.57
C


ATOM
4331
CG
PRO
C
121
76.380
94.189
−8.846
1.00
45.38
C


ATOM
4332
C
PRO
C
121
78.054
96.442
−6.637
1.00
48.97
C


ATOM
4333
O
PRO
C
121
78.066
97.631
−6.340
1.00
49.57
C


ATOM
4334
N
THR
C
122
78.361
95.485
−5.766
1.00
45.65
C


ATOM
4335
CA
THR
C
122
78.735
95.807
−4.388
1.00
48.81
C


ATOM
4336
CB
THR
C
122
78.209
94.712
−3.397
1.00
52.30
C


ATOM
4337
OG1
THR
C
122
76.777
94.615
−3.488
1.00
54.63
C


ATOM
4338
CG2
THR
C
122
78.563
95.064
−1.953
1.00
54.73
C


ATOM
4339
C
THR
C
122
80.264
95.881
−4.287
1.00
51.31
C


ATOM
4340
O
THR
C
122
80.964
95.769
−5.277
1.00
49.95
C


ATOM
4341
N
ARG
C
123
80.792
96.106
−3.094
1.00
56.40
C


ATOM
4342
CA
ARG
C
123
82.233
96.099
−2.936
1.00
54.17
C


ATOM
4343
CB
ARG
C
123
82.628
96.777
−1.621
1.00
62.98
C


ATOM
4344
CG
ARG
C
123
82.258
98.275
−1.561
1.00
73.33
C


ATOM
4345
CD
ARG
C
123
82.868
98.987
−0.336
1.00
77.96
C


ATOM
4346
NE
ARG
C
123
83.483
100.279
−0.674
1.00
80.89
C


ATOM
4347
CZ
ARG
C
123
84.558
100.439
−1.458
1.00
80.79
C


ATOM
4348
NH1
ARG
C
123
85.166
99.392
−2.007
1.00
79.18
C


ATOM
4349
NH2
ARG
C
123
85.035
101.656
−1.696
1.00
81.16
C


ATOM
4350
C
ARG
C
123
82.566
94.608
−2.890
1.00
52.07
C


ATOM
4351
O
ARG
C
123
81.687
93.780
−2.609
1.00
51.24
C


ATOM
4352
N
LEU
C
124
83.801
94.245
−3.210
1.00
46.24
C


ATOM
4353
CA
LEU
C
124
84.175
92.836
−3.137
1.00
43.03
C


ATOM
4354
CB
LEU
C
124
85.682
92.655
−3.342
1.00
38.28
C


ATOM
4355
CG
LEU
C
124
86.181
92.386
−4.766
1.00
39.78
C


ATOM
4356
CD1
LEU
C
124
87.714
92.314
−4.721
1.00
41.84
C


ATOM
4357
CD2
LEU
C
124
85.591
91.090
−5.304
1.00
41.07
C


ATOM
4358
C
LEU
C
124
83.785
92.371
−1.738
1.00
35.52
C


ATOM
4359
O
LEU
C
124
83.740
93.175
−0.807
1.00
32.32
C


ATOM
4360
N
PRO
C
125
83.473
91.080
−1.574
1.00
31.53
C


ATOM
4361
CD
PRO
C
125
83.668
89.969
−2.523
1.00
35.48
C


ATOM
4362
CA
PRO
C
125
83.093
90.568
−0.255
1.00
36.74
C


ATOM
4363
CB
PRO
C
125
82.663
89.127
−0.554
1.00
32.42
C


ATOM
4364
CG
PRO
C
125
83.629
88.741
−1.610
1.00
33.06
C


ATOM
4365
C
PRO
C
125
84.328
90.593
0.623
1.00
36.97
C


ATOM
4366
O
PRO
C
125
85.438
90.594
0.117
1.00
34.38
C


ATOM
4367
N
THR
C
126
84.130
90.571
1.930
1.00
41.39
C


ATOM
4368
CA
THR
C
126
85.239
90.584
2.857
1.00
44.67
C


ATOM
4369
CB
THR
C
126
85.171
91.881
3.691
1.00
49.35
C


ATOM
4370
OG1
THR
C
126
86.470
92.211
4.174
1.00
58.86
C


ATOM
4371
CG2
THR
C
126
84.227
91.731
4.855
1.00
46.39
C


ATOM
4372
C
THR
C
126
85.179
89.343
3.765
1.00
43.43
C


ATOM
4373
O
THR
C
126
84.101
88.820
4.068
1.00
41.61
C


ATOM
4374
N
CYS
C
127
86.333
88.832
4.164
1.00
46.42
C


ATOM
4375
CA
CYS
C
127
86.348
87.679
5.068
1.00
46.58
C


ATOM
4376
C
CYS
C
127
86.997
88.162
6.361
1.00
47.12
C


ATOM
4377
O
CYS
C
127
88.182
88.490
6.384
1.00
44.98
C


ATOM
4378
CB
CYS
C
127
87.153
86.515
4.485
1.00
41.09
C


ATOM
4379
SG
CYS
C
127
86.397
85.641
3.072
1.00
36.94
C


ATOM
4380
N
VAL
C
128
86.202
88.224
7.422
1.00
47.64
C


ATOM
4381
CA
VAL
C
128
86.671
88.675
8.725
1.00
50.09
C


ATOM
4382
CB
VAL
C
128
85.674
89.670
9.349
1.00
53.76
C


ATOM
4383
CG1
VAL
C
128
84.322
88.993
9.553
1.00
56.55
C


ATOM
4384
CG2
VAL
C
128
86.211
90.180
10.678
1.00
55.81
C


ATOM
4385
C
VAL
C
128
86.860
87.494
9.696
1.00
50.97
C


ATOM
4386
O
VAL
C
128
86.117
86.494
9.653
1.00
47.21
C


ATOM
4387
N
SER
C
129
87.860
87.611
10.563
1.00
44.79
C


ATOM
4388
CA
SER
C
129
88.146
86.561
11.534
1.00
41.23
C


ATOM
4389
CB
SER
C
129
89.373
86.901
12.370
1.00
36.76
C


ATOM
4390
OG
SER
C
129
89.483
85.955
13.423
1.00
38.45
C


ATOM
4391
C
SER
C
129
87.020
86.289
12.506
1.00
42.12
C


ATOM
4392
O
SER
C
129
86.396
87.214
13.030
1.00
38.42
C


ATOM
4393
N
VAL
C
130
86.783
85.004
12.747
1.00
42.88
C


ATOM
4394
CA
VAL
C
130
85.786
84.566
13.712
1.00
48.17
C


ATOM
4395
CB
VAL
C
130
85.679
83.028
13.724
1.00
49.37
C


ATOM
4396
CG1
VAL
C
130
85.006
82.570
15.003
1.00
55.87
C


ATOM
4397
CG2
VAL
C
130
84.879
82.558
12.510
1.00
47.26
C


ATOM
4398
C
VAL
C
130
86.191
85.042
15.124
1.00
47.25
C


ATOM
4399
O
VAL
C
130
85.334
85.271
15.976
1.00
49.24
C


ATOM
4400
N
PHE
C
131
87.495
85.202
15.360
1.00
42.64
C


ATOM
4401
CA
PHE
C
131
87.979
85.639
16.665
1.00
43.25
C


ATOM
4402
CB
PHE
C
131
89.429
85.174
16.873
1.00
37.67
C


ATOM
4403
CG
PHE
C
131
89.608
83.691
16.666
1.00
36.56
C


ATOM
4404
CD1
PHE
C
131
90.324
83.218
15.589
1.00
30.51
C


ATOM
4405
CD2
PHE
C
131
88.927
82.770
17.461
1.00
32.59
C


ATOM
4406
CE1
PHE
C
131
90.357
81.848
15.278
1.00
32.11
C


ATOM
4407
CE2
PHE
C
131
88.955
81.399
17.157
1.00
37.78
C


ATOM
4408
CZ
PHE
C
131
89.674
80.938
16.055
1.00
33.91
C


ATOM
4409
C
PHE
C
131
87.849
87.146
16.797
1.00
45.03
C


ATOM
4410
O
PHE
C
131
88.533
87.896
16.104
1.00
43.08
C


ATOM
4411
N
PRO
C
132
86.980
87.606
17.719
1.00
49.31
C


ATOM
4412
CD
PRO
C
132
86.384
86.810
18.806
1.00
48.64
C


ATOM
4413
CA
PRO
C
132
86.730
89.029
17.954
1.00
51.76
C


ATOM
4414
CB
PRO
C
132
85.882
89.026
19.227
1.00
51.00
C


ATOM
4415
CG
PRO
C
132
86.345
87.802
19.927
1.00
49.99
C


ATOM
4416
C
PRO
C
132
87.948
89.921
18.054
1.00
54.56
C


ATOM
4417
O
PRO
C
132
88.018
90.941
17.371
1.00
59.16
C


ATOM
4418
N
LEU
C
133
88.913
89.558
18.887
1.00
53.87
C


ATOM
4419
CA
LEU
C
133
90.089
90.396
18.991
1.00
56.98
C


ATOM
4420
CB
LEU
C
133
90.854
90.093
20.274
1.00
53.54
C


ATOM
4421
CG
LEU
C
133
89.997
90.162
21.538
1.00
52.81
C


ATOM
4422
CD1
LEU
C
133
90.901
90.183
22.748
1.00
53.95
C


ATOM
4423
CD2
LEU
C
133
89.142
91.398
21.540
1.00
53.34
C


ATOM
4424
C
LEU
C
133
90.958
90.156
17.772
1.00
58.59
C


ATOM
4425
O
LEU
C
133
92.106
90.579
17.726
1.00
54.07
C


ATOM
4426
N
GLU
C
134
91.054
89.741
16.857
1.00
60.78
C


ATOM
4427
CA
GLU
C
134
91.010
89.158
15.522
1.00
70.26
C


ATOM
4428
CB
GLU
C
134
91.448
90.184
14.475
1.00
72.39
C


ATOM
4429
CG
GLU
C
134
90.575
91.428
14.421
1.00
75.60
C


ATOM
4430
CD
GLU
C
134
89.133
91.113
14.077
1.00
78.38
C


ATOM
4431
OE1
GLU
C
134
88.819
89.923
13.866
1.00
80.20
C


ATOM
4432
OE2
GLU
C
134
88.317
92.057
14.018
1.00
80.22
C


ATOM
4433
C
GLU
C
134
91.879
87.908
15.438
1.00
71.86
C


ATOM
4434
O
GLU
C
134
92.143
87.521
16.835
1.00
73.34
C


ATOM
4435
OXT
GLU
C
134
92.658
87.662
14.702
1.00
76.05
C


ATOM
4436
C1
NAG
D
1
91.022
77.190
13.373
1.00
39.02
D


ATOM
4437
O1
NAG
D
1
90.217
76.061
13.510
1.00
53.38
D


ATOM
4438
C2
NAG
D
1
92.379
76.764
12.861
1.00
36.79
D


ATOM
4439
N2
NAG
D
1
92.264
76.234
11.516
1.00
39.67
D


ATOM
4440
C7
NAG
D
1
92.851
76.904
10.527
1.00
40.49
D


ATOM
4441
O7
NAG
D
1
93.458
77.963
10.726
1.00
46.19
D


ATOM
4442
C8
NAG
D
1
92.771
76.338
9.115
1.00
36.88
D


ATOM
4443
C3
NAG
D
1
92.972
75.726
13.816
1.00
35.88
D


ATOM
4444
O3
NAG
D
1
94.293
75.444
13.394
1.00
40.30
D


ATOM
4445
C4
NAG
D
1
92.986
76.292
15.264
1.00
37.37
D


ATOM
4446
O4
NAG
D
1
93.308
75.255
16.169
1.00
36.52
D


ATOM
4447
C5
NAG
D
1
91.610
76.877
15.659
1.00
38.38
D


ATOM
4448
O5
NAG
D
1
91.167
77.818
14.663
1.00
39.81
D


ATOM
4449
C6
NAG
D
1
91.599
77.601
16.994
1.00
34.64
D


ATOM
4450
O6
NAG
D
1
92.510
78.695
16.984
1.00
35.80
D


ATOM
4451
C1
NAG
E
1
90.580
70.350
4.612
1.00
80.29
E


ATOM
4452
O1
NAG
E
1
90.608
71.734
4.755
1.00
82.28
E


ATOM
4453
C2
NAG
E
1
91.606
69.705
5.560
1.00
79.89
E


ATOM
4454
N2
NAG
E
1
91.383
70.221
6.901
1.00
79.48
E


ATOM
4455
C7
NAG
E
1
90.709
69.507
7.799
1.00
79.52
E


ATOM
4456
O7
NAG
E
1
89.865
68.652
7.495
1.00
78.01
E


ATOM
4457
C8
NAG
E
1
91.027
69.791
9.263
1.00
77.64
E


ATOM
4458
C3
NAG
E
1
93.061
70.018
5.141
1.00
79.82
E


ATOM
4459
O3
NAG
E
1
93.945
69.116
5.805
1.00
80.58
E


ATOM
4460
C4
NAG
E
1
93.291
69.924
3.618
1.00
80.30
E


ATOM
4461
O4
NAG
E
1
94.526
70.547
3.285
1.00
80.30
E


ATOM
4462
C5
NAG
E
1
92.148
70.607
2.853
1.00
80.10
E


ATOM
4463
O5
NAG
E
1
90.884
70.035
3.252
1.00
79.60
E


ATOM
4464
C6
NAG
E
1
92.253
70.476
1.341
1.00
80.36
E


ATOM
4465
O6
NAG
E
1
91.044
70.874
0.705
1.00
78.94
E


END









While various embodiments of the present invention have been described in detail, it is apparent that modifications and adaptations of those embodiments will occur to those skilled in the art. It is to be expressly understood, however, that such modifications and adaptations are within the scope of the present invention, as set forth in the following claims.

## Claims

1. A method of inhibiting Epstein Barr Virus (EBV) infection in a human, comprising administering to the human an isolated antibody or antigen-binding fragment thereof that selectively binds to complement receptor type 2 (CR2) and inhibits the binding of CR2 to EBV, wherein the antibody or antigen-binding fragment thereof binds to the same epitope of CR2 as the monoclonal antibody mAb171 (produced by ATCC deposit PTA-12091).

2. A method of inhibiting Epstein Barr Virus (EBV) infection in a human, comprising administering to the human an isolated antibody or antigen-binding fragment thereof that selectively binds to complement receptor type 2 (CR2) and inhibits the binding of CR2 to EBV, wherein the antibody or antigen-binding fragment thereof competitively inhibits the specific binding of the monoclonal antibody mAb171 (produced by ATCC deposit PTA-12091) to CR2.

3. The method of claim 1, wherein the isolated antibody or antigen-binding fragment thereof inhibits the binding of CR2 to C3d.

4. The method of claim 1, wherein the method comprises administering said antigen-binding fragment.

5. The method of claim 4, wherein said antigen-binding fragment is selected from the group consisting of an Fv fragment, an Fab fragment, an Fab' fragment, and an F(ab)2 fragment.

6. The method of claim 1, wherein the method comprises administering said antibody.

7. The method of claim 6, wherein said antibody is a monoclonal antibody.

8. The method of claim 1, wherein said antibody is a single-chain antibody or antigen-binding fragment thereof.

9. A method of inhibiting Epstein Barr Virus (EBV) infection in a human, comprising administering to the human an isolated bispecific antibody or antigen-binding fragment thereof that selectively binds to complement receptor type 2 (CR2) and inhibits the binding of CR2 to EBV, wherein the bispecific antibody or antigen-binding fragment thereof binds to the same epitope of CR2 as the monoclonal antibody mAb171 (produced by ATCC deposit PTA-12091).

10. The method of claim 1, wherein said antibody or antigen-binding fragment thereof is a chimeric antibody or antigen-binding fragment thereof.

11. The method of claim 1, wherein said antibody or antigen-binding fragment thereof is a CDR grafted antibody or antigen-binding fragment thereof.

12. The method of claim 1, wherein said antibody or antigen-binding fragment thereof is mAb171 (produced by ATCC deposit PTA-12091) or antigen-binding fragment thereof.

13. The method of claim 1, wherein said antibody or antigen-binding fragment thereof is a chimeric antibody or antigen-binding fragment thereof originated from mAb171 (produced by ATCC deposit PTA-12091).