# Patent

## Title
Quinazoline compounds

## Abstract

Disclosed are compounds having the formula: wherein R1, R2, R3, R4, R5, R6 and R7 are as defined herein, and methods of making and using the same.

## Background
This application is a §371 of International Application No. PCT/US2010/054927, filed 01 Nov. 2010, which claims the benefit of U.S. Provisional Application No. 61/257,646, filed 03 Nov. 2009, which are incorporated herein in their entireties.


FIELD OF THE INVENTION
The present invention relates to compounds that inhibit TNNI3K and methods of making and using the same. Specifically, the present invention relates to quinazolines as TNNI3K inhibitors.
BACKGROUND OF THE INVENTION
Cardiac troponin I-interacting kinase (TNNI3K), also known as CARK (for cardiac ankyrin repeat kinase), is a protein kinase that exhibits highly selective expression for cardiac tissues and has been shown to interact with components of the sarcomere, including troponin I (Zhao, Y. et al., J. Mol. Med., 2003, 81, 297-304; Feng, Y. et al., Gen. Physiol. Biophys., 2007, 26, 104-109; Wang, H. et al., J. Cell. Mol. Med., 2008, 12, 304-315). Although substrates for TNNI3K have not been identified to date, recent reports suggest that this protein does play a role in the development of pressure-induced cardiomyocyte hypertrophy and contractile dysfunction (Wheeler, F. C. et al., Mamm. Genome, 2005, 16, 414-423; Wang, X. et al. “TNNI3K, a cardiac-specific kinase, promotes cardiac hypertrophy in vivo”, Poster presentation at the 2006 Scientific Sessions of the American Heart Association, Chicago, Ill., Wheeler, F. C. et al., PLos Genet, 2009, 5(9), e1000647; and Pu, W. T., PLos Genet, 2009, 5(9), e1000643). Inhibition of the kinase activity of TNNI3K may disrupt these signaling pathways, and enable the mitigation and/or reversal of cardiac hypertrophy seen in patients with progressively worsening heart failure.
In response to mechanical, neurohormonal, and genetic stimuli, the heart will undergo hypertrophy, or muscle growth and remodeling, in order to maintain sufficient cardiac output to meet tissue oxygen demands. While these structural changes are initially seen as compensatory, sustained dysregulation of hypertrophic signaling can lead to heart failure, the pathophysiogical state in which the heart can no longer adequately function as a pump (Mudd, J. O. and Kass, D. A., Nature, 2008, 451, 919-928). Prevention or reversal of pathological cardiac hypertrophy has the potential to delay or prevent the development of congestive heart failure (McKinsey, T. A. and Kass, D. A., Nat. Rev. Drug Discov., 2007, 6, 617-635; Kaye, D. M. and Krum, H., Nat. Rev. Drug Discov., 2007, 6, 127-139).
Heart failure is responsible for a reduced quality of life and premature death in a significant proportion of sufferers, and is characterized by impaired cardiac function either due to reduced pump function (systolic dysfunction) or reduced filling (diastolic dysfunction). Congestive heart failure (CHF) is characterized by impaired left ventricular function, increased peripheral and pulmonary vascular resistance and reduced exercise tolerance and dyspnea. The prevalence of heart failure is anticipated to increase with aging populations, prompting a need for new and improved methods of treating heart failure.
SUMMARY OF THE INVENTION
The invention is directed to novel quinazolines. Specifically, the invention is directed to a compound according to Formula I:




wherein:

R1 is (C1-C4)alkyl;
R2 is H or halogen;
R3 is H, halogen, (C1-C8)alkyl, (C1-C8)haloalkyl, hydroxyl, hydroxy(C1-C8)alkyl-, (C1-C8)alkoxy, (C3-C8)cycloalkyloxy, (C1-C8)haloalkoxy, (C1-C8)alkylthio-, (C1-C8)haloalkylthio-, (C3-C8)cycloalkylthio-, (C1-C8)alkylsulfonyl, (C1-C8)haloalkylsulfonyl, (C3-C8)cycloalkylsulfonyl, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb),
wherein said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, (C1-C6)alkoxy, (C1-C4)haloalkoxy, hydroxy(C1-C4)alkyl-, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C6)alkoxy, amino, (C1-C6)alkylamino, ((C1-C6)alkyl)((C1-C6)alkyl)amino, —CO2H, —CO2(C1-C6)alkyl, —CONH2, —CONH(C1-C6)alkyl, or —CON((C1-C6)alkyl)((C1-C6)alkyl), and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C1-C4)alkyl, (C1-C4)haloalkyl, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, hydroxy(C1-C4)alkyl-, oxo, (C1-C4)alkoxy, (C1-C4)haloalkoxy, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc; and
each Rc is, independently, (C1-C8)alkyl or a 5-6 membered heterocycloalkyl, wherein said (C1-C8)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, or —N(Ra)(Rb);
or R6 and R7 taken together represent —O(C1-C2)alkylO—;
or a salt, particularly a pharmaceutically acceptable salt, thereof.
The compounds of the invention are inhibitors of TNNI3K and can be useful for the treatment of cardiac diseases and disorders, particularly heart failure. Accordingly, the invention is further directed to pharmaceutical compositions comprising a compound of the invention. The invention is still further directed to methods of inhibiting TNNI3K and treatment of conditions associated therewith using a compound of the invention or a pharmaceutical composition comprising a compound of the invention.


DETAILED DESCRIPTION OF THE INVENTION
As used herein, the term “alkyl” represents a saturated, straight or branched hydrocarbon moiety, which may be unsubstituted or substituted by one, or more of the substituents defined herein. Exemplary alkyls include, but are not limited to methyl (Me), ethyl (Et), propyl, isopropyl, butyl, isobutyl, t-butyl and pentyl. The term “C1-C4” refers to an alkyl containing from 1 to 4 carbon atoms.
When the term “alkyl” is used in combination with other substituent groups, such as “haloalkyl” or “hydroxyalkyl” or “arylalkyl”, the term “alkyl” is intended to encompass a divalent straight or branched-chain hydrocarbon radical. For example, “arylalkyl” is intended to mean the radical—alkylaryl, wherein the alkyl moiety thereof is a divalent straight or branched-chain carbon radical and the aryl moiety thereof is as defined herein, and is represented by the bonding arrangement present in a benzyl group (—CH2-phenyl).
As used herein, the term “cycloalkyl” refers to a non-aromatic, saturated, cyclic hydrocarbon ring. The term “(C3-C8)cycloalkyl” refers to a non-aromatic cyclic hydrocarbon ring having from three to eight ring carbon atoms. Exemplary “(C3-C8)cycloalkyl” groups useful in the present invention include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
“Alkoxy” refers to a group containing an alkyl radical attached through an oxygen linking atom. The term “(C1-C4)alkoxy” refers to a straight- or branched-chain hydrocarbon radical having at least 1 and up to 4 carbon atoms attached through an oxygen linking atom. Exemplary “(C1-C4)alkoxy” groups useful in the present invention include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, s-butoxy, and t-butoxy.
“Alkylthio-” refers to a group containing an alkyl radical attached through a sulfur linking atom. The term “(C1-C4)alkylthio-” refers to a straight- or branched-chain hydrocarbon radical having at least 1 and up to 4 carbon atoms attached through a sulfur linking atom. Exemplary “(C1-C4)alkylthio-” groups useful in the present invention include, but are not limited to, methylthio-, ethylthio-, n-propylthio-, isopropylthio-, n-butylthio-, s-butylthio-, and t-butylthio-.
“Alkylsulfonyl” refers to a group containing an alkyl radical attached through a sulfonyl radical (i.e. —S(═O)2—). The term “(C1-C4)alkylsulfonyl” refers to a straight- or branched-chain hydrocarbon radical having at least 1 and up to 4 carbon atoms attached through a sulfonyl radical. Exemplary “(C1-C4)alkylsulfonyl” groups useful in the present invention include, but are not limited to, methanesulfonyl, ethanesulfonyl, propanesulfonyl, and butanesulfonyl.
“Cycloalkyloxy” and “cycloalkylthio” refers to a group containing a saturated carbocyclic ring atoms attached through an oxygen or sulfur linking atom, respectively. Examples of “cycloalkyloxy” moieties include, but are not limited to, cyclopropoxy, cyclobutoxy, cyclopentyloxy, cyclohexyloxy, and the like.
“Aryl” represents a group or moiety comprising an aromatic, monovalent monocyclic or bicyclic hydrocarbon radical containing from 6 to 10 carbon ring atoms, which may be unsubstituted or substituted by one or more of the substituents defined herein, and to which may be fused one or more cycloalkyl rings, which may be unsubstituted or substituted by one or more substituents defined herein.
Generally, in the compounds of this invention, aryl is phenyl.
Heterocyclic groups may be heteroaryl or heterocycloalkyl groups.
“Heterocycloalkyl” represents a group or moiety comprising a non-aromatic, monovalent monocyclic or bicyclic radical, which is saturated or partially unsaturated, containing 3 to 10 ring atoms, which includes 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, and which may be unsubstituted or substituted by one or more of the substituents defined herein. Illustrative examples of heterocycloalkyls include, but are not limited to, azetidinyl, pyrrolidyl (or pyrrolidinyl), piperidinyl, piperazinyl, morpholinyl, tetrahydro-4H-1,4-thiazinyl, tetrahydrofuryl (or tetrahydrofuranyl), dihydrofuryl, oxazolinyl, thiazolinyl, pyrazolinyl, tetrahydropyranyl, dihydropyranyl, 1,3-dioxolanyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-oxathiolanyl, 1,3-oxathianyl, 1,3-dithianyl, azabicylo[3.2.1]octyl, azabicylo[3.3.1]nonyl, azabicylo[4.3.0]nonyl, oxabicylo[2.2.1]heptyl and 1,5,9-triazacyclododecyl.
Generally, in the compounds of this invention, heterocycloalkyl groups are 5-membered and/or 6-membered heterocycloalkyl groups, such as pyrrolidyl (or pyrrolidinyl), tetrahydrofuryl (or tetrahydrofuranyl), tetrahydrothienyl, dihydrofuryl, oxazolinyl, thiazolinyl, pyrazolinyl, piperidyl (or piperidinyl), piperazinyl, morpholinyl, tetrahydropyranyl, dihydropyranyl, 1,3-dioxanyl, tetrahydro-4H-1,4-thiazinyl, 1,4-dioxanyl, 1,3-oxathianyl, and 1,3-dithianyl.
“Heteroaryl” represents a group or moiety comprising an aromatic monovalent monocyclic or bicyclic radical, containing 5 to 10 ring atoms, including 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, which may be unsubstituted or substituted by one or more of the substituents defined herein. This term also encompasses bicyclic heterocyclic-aryl compounds containing an aryl ring moiety fused to a heterocycloalkyl ring moiety, containing 5 to 10 ring atoms, including 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, which may be unsubstituted or substituted by one or more of the substituents defined herein. Illustrative examples of heteroaryls include, but are not limited to, thienyl, pyrrolyl, imidazolyl, pyrazolyl, furyl (or furanyl), isothiazolyl, isoxazolyl, oxazolyl, oxadiazolyl, thiazolyl, pyridyl (or pyridinyl), pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, tetrazinyl, triazolyl, tetrazolyl, benzo[b]thienyl, isobenzofuryl, 2,3-dihydrobenzofuryl, chromenyl, chromanyl, indolizinyl, isoindolyl, indolyl, indazolyl, purinyl, isoquinolyl, quinolyl, phthalazinyl, naphthridinyl, quinazolinyl, benzothiazolyl, benzimidazolyl, tetrahydroquinolinyl, cinnolinyl, pteridinyl, and isothiazolyl.
Generally, the heteroaryl groups present in the compounds of this invention are 5-membered and/or 6-membered monocyclic heteroaryl groups. Selected 5-membered heteroaryl groups contain one nitrogen, oxygen or sulfur ring heteroatom, and optionally contain 1, 2 or 3 additional nitrogen ring atoms. Selected 6-membered heteroaryl groups contain 1, 2, 3 or 4 nitrogen ring heteroatoms. Selected 5- or 6-membered heteroaryl groups include thienyl, pyrrolyl, imidazolyl, pyrazolyl, furyl, isothiazolyl, isoxazolyl, oxazolyl, oxadiazolyl, thiazolyl, triazolyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, and triazinyl.
“Oxo” represents a double-bonded oxygen moiety; for example, if attached directly to a carbon atom forms a carbonyl moiety (C═O).
The terms “halogen” and “halo” represent chloro, fluoro, bromo or iodo substituents. “Hydroxy” or “hydroxyl” is intended to mean the radical —OH.
As used herein, the term “compound(s) of the invention” means a compound of formula (I) (as defined above) in any form, i.e., any salt or non-salt form (e.g., as a free acid or base form, or as a pharmaceutically acceptable salt thereof) and any physical form thereof (e.g., including non-solid forms (e.g., liquid or semi-solid forms), and solid forms (e.g., amorphous or crystalline forms, specific polymorphic forms, solvates, including hydrates (e.g., mono-, di- and hemi-hydrates)), and mixtures of various forms.
As used herein, the term “optionally substituted” means that the groups may be either unsubstituted or substituted with one or more of the specified substituents.
The alternative definitions for the various groups and substituent groups of Formula I provided throughout the specification are intended to particularly describe each compound species disclosed herein, individually, as well as groups of one or more compound species. The scope of this invention includes any combination of these group and substituent group definitions.
The invention is further directed to a compound according to Formula I, wherein:
R1 is (C1-C4)alkyl;
R2 is H or halogen;
R3 is H, halogen, (C1-C4)alkoxy, (C5-C6)cycloalkyloxy, (C1-C4)haloalkoxy, (C1-C4)alkylthio-, (C1-C4)haloalkylthio-, (C5-C6)cycloalkylthio-, (C1-C4)alkylsulfonyl, (C1-C4)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, or —N(Ra)(Rb); each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, —CO2H, —CO2(C1-C4)alkyl, —CONH2, —CONH(C1-C4)alkyl, or —CON((C1-C4)alkyl)((C1-C4)alkyl), and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C1-C4)alkyl, (C1-C4)haloalkyl, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, hydroxy(C1-C4)alkyl-, oxo, (C1-C4)alkoxy, (C1-C4)haloalkoxy, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc; and
each Rc is, independently, (C1-C4)alkyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl, wherein said (C1-C4)alkyl is optionally substituted by halogen, hydroxyl, trifluoromethyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl;
or R6 and R7 taken together represent —O(C1-C2)alkylO—;
or a salt, particularly a pharmaceutically acceptable salt, thereof.
In another embodiment of this invention:
R1 is (C1-C4)alkyl;
R2 is H;
R3 is H, halogen, (C1-C8)alkyl, (C1-C8)haloalkyl, hydroxyl, hydroxy(C1-C8)alkyl-, (C1-C8)alkoxy, (C3-C8)cycloalkyloxy, (C1-C8)haloalkoxy, (C1-C8)alkylthio-, (C1-C8)haloalkylthio-, (C3-C8)cycloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb),
wherein said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, (C1-C6)alkoxy, (C1-C4)haloalkoxy, hydroxy(C1-C4)alkyl-, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C6)alkoxy, amino, (C1-C6)alkylamino, ((C1-C6)alkyl)((C1-C6)alkyl)amino, —CO2H, —CO2(C1-C6)alkyl, —CONH2, —CONH(C1-C6)alkyl, or —CON((C1-C6)alkyl)((C1-C6)alkyl), and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C1-C4)alkyl, (C1-C4)haloalkyl, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, hydroxy(C1-C4)alkyl-, oxo, (C1-C4)alkoxy, (C1-C4)haloalkoxy, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5 is H;
R6 and R7 are each ORc; and
each Rc is, independently, (C1-C8)alkyl, optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, or ((C1-C4)alkyl)((C1-C4)alkyl)amino;
or a salt, particularly a pharmaceutically acceptable salt, thereof.
In another embodiment of this invention:
R1 is (C1-C3 alkyl);
R2 is H;
R3 is H, halogen, C1-C6 alkyl, C1-C6 haloalkyl, hydroxyC1-C6 alkyl-, C1-C6 alkoxy, (C3-C6)cycloalkyloxy, C1-C6 haloalkoxy, C1-C6 alkylthio-, (C3-C6)cycloalkylthio-, C1-C6 haloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb),
wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted by hydroxyl, trifluoromethyl, (C1-C6)alkoxy, amino, (C1-C6)alkylamino, or ((C1-C6)alkyl)((C1-C6)alkyl)amino, and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by (C1-C4)alkyl, (C1-C4)haloalkyl, hydroxy(C1-C4)alkyl-, oxo, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5 is H;
R6 and R7 are each ORc; and
each Rc is, independently, (C1-C4)alkyl, optionally substituted one to three times, independently, by halogen;
or a salt, particularly a pharmaceutically acceptable salt, thereof.
In a specific embodiment of this invention, R1 is —CH3.
In another specific embodiment of this invention, R2 is H or F. In a further specific embodiment of this invention, R2 is H.
In another embodiment of the invention, R3 is H, halogen, (C1-C6)alkyl, (C1-C6)haloalkyl, (C1-C6)alkoxy, (C5-C6)cycloalkyloxy, (C1-C6)haloalkoxy, (C1-C6)alkylthio-, (C5-C6)cycloalkylthio-, (C1-C6)haloalkylthio-, (C1-C6)alkylsulfonyl, (C1-C6)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb).
In a further embodiment of this invention, R3 is H, halogen, (C1-C8)alkyl, (C1-C8)haloalkyl, hydroxyl, hydroxy(C1-C8)alkyl-, (C1-C8)alkoxy, (C3-C8)cycloalkyloxy, (C1-C8)haloalkoxy, (C1-C8)alkylthio-, (C1-C8)haloalkylthio-, (C3-C8)cycloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, (C1-C6)alkoxy, (C1-C4)haloalkoxy, hydroxy(C1-C4)alkyl-, or —N(Ra)(Rb).
In yet a further embodiment of this invention, R3 is H, halogen, C1-C6 alkyl, C1-C6 haloalkyl, hydroxyC1-C6 alkyl-, C1-C6 alkoxy, (C3-C6)cycloalkyloxy, C1-C6 haloalkoxy, C1-C6 alkylthio-, (C3-C6)cycloalkylthio-, C1-C6 haloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb).
In yet a further embodiment of this invention, R3 is H, halogen, (C1-C4)alkoxy, (C5-C6)cycloalkyloxy, (C1-C4)haloalkoxy, (C1-C4)alkylthio-, (C1-C4)haloalkylthio-, (C5-C6)cycloalkylthio-, (C1-C4)alkylsulfonyl, (C1-C4)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, or —N(Ra)(Rb).
In a specific embodiment of this invention, each Ra is independently —CH3, —CH2CH3, or —CH2CF3, and Rb is —CH3; or Ra and Rb taken together with the nitrogen to which they are attached represent pyrrolidin-1-yl, piperidin-1-yl, or morpholin-4-yl, wherein said pyrrolidin-1-yl, piperidin-1-yl, or morpholin-4-yl is optionally substituted one or two times, independently, by F, —CH3, or —CF3.
In another specific embodiment of this invention, R3 is H, F, Cl, —OCH3, —OCH(CH3)2, —OCF3, —OCH2CF3, —OCH(CH3)CF3, —SCH3, —SCH2CF3, —SO2CH3, —N(CH3)2, —N(CH3)CH2CH3, —N(CH3)CH2CF3, 2-(trifluoromethyl)pyrrolidin-1-yl, 2,5-(dimethyl)pyrrolidin-1-yl, piperidin-1-yl, 4,4-difluoro-piperidin-1-yl, or morpholin-4-yl.
In a further embodiment of this invention, R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc, and each Rc is, independently, (C1-C4)alkyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl, wherein said (C1-C4)alkyl is optionally substituted by halogen, hydroxyl, trifluoromethyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl; or R6 and R7 taken together represent —O(C1-C2)alkylO—.
In a specific embodiment of this invention, R5 is H, Cl, —OCH3, —OCH2CH3, —OCH(CH3)2, —O(CH2)2OCH3, or —O(tetrahydropyran-4-yl).
In another specific embodiment of this invention, R5 is H.
In another embodiment of this invention, R6 and R7 are each ORc; and each Rc is, independently, (C1-C8)alkyl, optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, or ((C1-C4)alkyl)((C1-C4)alkyl)amino.
In a further embodiment of this invention, R6 and R7 are each ORc; and each Rc is, independently, (C1-C4)alkyl, optionally substituted one to three times, independently, by halogen.
In yet a further embodiment of this invention, R6 and R7 are each ORc; and each Rc is, independently, (C1-C4)alkyl, optionally substituted by (C1-C4)alkoxy.
In a specific embodiment of this invention, R6 is H, Cl, I, —OCH3, —OCH2CH3, —OCH(CH3)2, —O(CH2)2OCH3, —O(CH2)3-morpholin-4-yl, —NH2, —NHCH3, or —N(CH3)2.
In another specific embodiment of this invention, R6 is H.
In another specific embodiment of this invention, R7 is H, —OH, —OCH3, —OCH2CH3, —OCH(CH3)2, —O(CH2)3Cl, —O(CH2)2OCH3, —O(CH2)3OCH3, —O(C H2)3— morpholin-4-yl, —NH2, —NHCH3, or —NO2.
In another specific embodiment of this invention, R6 and R7 taken together represent —O(CH2)2O—.
Specific compounds of this invention are:
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(4-morpholinyl)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-[ethyl(methyl)amino]-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(1-methylethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylthio)benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-(4-quinazolinylamino)benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(dimethylamino)-N-methylbenzenesulfonamide;
4-(dimethylamino)-N-methyl-3-{[6-(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-(7,8-dihydro[1,4]dioxino[2,3-g]quinazolin-4-ylamino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(4,4-difluoro-1-piperidinyl)-N-methylbenzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(4,4-difluoro-1-piperidinyl)-N-methylbenzenesulfonamide;
3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-(methyloxy)benzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-(methylthio)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2R)-2-(trifluoromethyl)-1-pyrrolidinyl]benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-[(2R)-2-(trifluoromethyl)-1-pyrrolidinyl]benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(1-piperidinyl)benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-(1-piperidinyl)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(2,5-dimethyl-1-pyrrolidinyl)-N-methylbenzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(2,5-dimethyl-1-pyrrolidinyl)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)thio]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-(5-chloro-6,7-dimethoxyquinazolin-4-ylamino)-N-methylbenzenesulfonamide;
4-methoxy-3-(7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-ylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-fluoro-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-chloro-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(4-morpholinyl)benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[6-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-3-(7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-ylamino)-N-methylbenzenesulfonamide;
N-methyl-3-{[6-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-4-[(2,2,2-trifluoroethyl)oxy]-3-{[5, 6, 7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-(methyloxy)benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylthio)benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
N-methyl-4-[(trifluoromethyl)oxy]-3-{[5, 6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide;
2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]-5-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
4-chloro-N-methyl-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
N-methyl-3-[(7-(methyloxy)-5-{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-{[7-(methyloxy)-5-(tetrahydro-2H-pyran-4-yloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzesulfonamide;
N-methyl-4-(methylsulfonyl)-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(trifluoromethyl)oxy]benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[6-iodo-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(ethyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-((7-nitroquinazolin-4-yl)amino)benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide;
N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide;
3-(7-hydroxy-6-methoxyquinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoroethoxy)benzenesulfonamide;
3-(7-isopropoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-(7-ethoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-[6-methoxy-7-(2-methoxy-ethoxy)-quinazolin-4-ylamino]-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-((6-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-((7-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
4-(dimethylamino)-3-((6-(dimethylamino)quinazolin-4-yl)amino)-N-methylbenzenesulfonamide;
4-(dimethylamino)-N-methyl-3-((6-(methylamino)quinazolin-4-yl)amino)benzenesulfonamide; and
4-(dimethylamino)-N-methyl-3-((7-(methylamino)quinazolin-4-yl)amino)benzenesulfonamide;
and salts, particularly pharmaceutically acceptable salts, thereof.
Representative compounds of this invention include the compounds of Examples 1-88.
The compounds according to Formula I may contain one or more asymmetric center (also referred to as a chiral center) and may, therefore, exist as individual enantiomers, diastereomers, or other stereoisomeric forms, or as mixtures thereof. Chiral centers, such as chiral carbon atoms, may also be present in a substituent such as an alkyl group. Where the stereochemistry of a chiral center present in Formula I, or in any chemical structure illustrated herein, is not specified the structure is intended to encompass all individual stereoisomers and all mixtures thereof. Thus, compounds according to Formula I containing one or more chiral center may be used as racemic mixtures, enantiomerically enriched mixtures, or as enantiomerically pure individual stereoisomers.
Individual stereoisomers of a compound according to Formula I which contain one or more asymmetric center may be resolved by methods known to those skilled in the art. For example, such resolution may be carried out (1) by formation of diastereoisomeric salts, complexes or other derivatives; (2) by selective reaction with a stereoisomer-specific reagent, for example by enzymatic oxidation or reduction; or (3) by gas-liquid or liquid chromatography in a chiral environment, for example, on a chiral support such as silica with a bound chiral ligand or in the presence of a chiral solvent. The skilled artisan will appreciate that where the desired stereoisomer is converted into another chemical entity by one of the separation procedures described above, a further step is required to liberate the desired form. Alternatively, specific stereoisomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer to the other by asymmetric transformation.
When a disclosed compound or its salt is named or depicted by structure, it is to be understood that the compound or salt, including solvates (particularly, hydrates) thereof, may exist in crystalline forms, non-crystalline forms or a mixture thereof. The compound or salt, or solvates (particularly, hydrates) thereof, may also exhibit polymorphism (i.e. the capacity to occur in different crystalline forms). These different crystalline forms are typically known as “polymorphs.” It is to be understood that when named or depicted by structure, the disclosed compound, or solvates (particularly, hydrates) thereof, also include all polymorphs thereof. Polymorphs have the same chemical composition but differ in packing, geometrical arrangement, and other descriptive properties of the crystalline solid state. Polymorphs, therefore, may have different physical properties such as shape, density, hardness, deformability, stability, and dissolution properties. Polymorphs typically exhibit different melting points, IR spectra, and X-ray powder diffraction patterns, which may be used for identification. One of ordinary skill in the art will appreciate that different polymorphs may be produced, for example, by changing or adjusting the conditions used in crystallizing/recrystallizing the compound.
For solvates of the compounds of the invention, or salts thereof, that are in crystalline form, the skilled artisan will appreciate that pharmaceutically-acceptable solvates may be formed wherein solvent molecules are incorporated into the crystalline lattice during crystallization. Solvates may involve nonaqueous solvents such as ethanol, isopropanol, DMSO, acetic acid, ethanolamine, and ethyl acetate, or they may involve water as the solvent that is incorporated into the crystalline lattice. Solvates wherein water is the solvent that is incorporated into the crystalline lattice are typically referred to as “hydrates.” Hydrates include stoichiometric hydrates as well as compositions containing variable amounts of water. The invention includes all such solvates.
Because of their potential use in medicine, the salts of the compounds of Formula I are preferably pharmaceutically acceptable. The compounds of this invention are bases, wherein a desired salt form may be prepared by any suitable method known in the art, including treatment of the free base with an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like, or with an organic acid, such as acetic acid, trifluoroacetic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, pyranosidyl acid, such as glucuronic acid or galacturonic acid, alpha-hydroxy acid, such as citric acid or tartaric acid, amino acid, such as aspartic acid or glutamic acid, aromatic acid, such as benzoic acid or cinnamic acid, sulfonic acid, such as p-toluenesulfonic acid, methanesulfonic acid, ethanesulfonic acid or the like. Examples of pharmaceutically acceptable salts include sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, phosphates, chlorides, bromides, iodides, acetates, propionates, decanoates, caprylates, acrylates, formates, isobutyrates, caproates, heptanoates, propiolates, oxalates, malonates succinates, suberates, sebacates, fumarates, maleates, butyne-1,4-dioates, hexyne-1,6-dioates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, hydroxybenzoates, methoxybenzoates, phthalates, phenylacetates, phenylpropionates, phenylbutrates, citrates, lactates, γ-hydroxybutyrates, glycollates, tartrates mandelates, and sulfonates, such as xylenesulfonates, methanesulfonates, propanesulfonates, naphthalene-1-sulfonates and naphthalene-2-sulfonates.
If an inventive basic compound is isolated as a salt, the corresponding free base form of that compound may be prepared by any suitable method known to the art, including treatment of the salt with an inorganic or organic base, suitably an inorganic or organic base having a higher pKa than the free base form of the compound.
General Methods of Preparation
The compounds of Formula I may be obtained by using synthetic procedures illustrated in the Schemes below or by drawing on the knowledge of a skilled organic chemist. The synthesis provided in these Schemes are applicable for producing compounds of the invention having a variety of different R1 and R2 groups employing appropriate precursors, which are suitably protected if needed, to achieve compatibility with the reactions outlined herein. Subsequent deprotection, where needed, affords compounds of the nature generally disclosed. While the Schemes are shown with compounds only of Formula I, they are illustrative of processes that may be used to make the compounds of the invention.
Compound names were generated using the software naming program ACD/Name Pro V6.02 available from Advanced Chemistry Development, Inc., 110 Yonge Street, 14th Floor, Toronto, Ontario, Canada, M5C 1T4 (http://www.acdlabs.com/).
As shown in Scheme 1, the compounds of Formula I can be prepared under a variety of conditions by reaction of an arylamine (e.g., Ar—NH—R2, specifically, Ar—NH2) with an activated quinazoline.




As shown in Schemes 2-5, the compounds of Formula I can be prepared via common functional group transformations on advanced intermediates.
















The invention also includes various deuterated forms of the compounds of Formula I. Each available hydrogen atom attached to a carbon atom may be independently replaced with a deuterium atom. A person of ordinary skill in the art will know how to synthesize deuterated forms of the compounds of Formula I. For example, deuterated alkyl groups (e.g., N-(deuteromethyl)amines or Ra/Rb alkyls) may be prepared by conventional techniques (see for example: methyl-d3-amine available from Aldrich Chemical Co., Milwaukee, Wis., Cat. No. 489,689-2). Employing such compounds according to Scheme 1 will allow for the preparation of compounds of Formula I in which various hydrogen atoms of the N-methyl or pyrimidinyl groups are replaced with a deuterium atom.
Methods of Use
The present invention is directed to a method of inhibiting TNNI3K which comprises contacting the kinase with a compound of Formula I or a salt thereof, particularly a pharmaceutically acceptable salt thereof. This invention is also directed to a method of treatment of a TNNI3K-mediated disease or disorder comprising administering an effective amount of the compound of Formula I or a salt thereof, particularly a pharmaceutically acceptable salt thereof, to a patient, specifically a human, in need thereof. As used herein, “patient” refers to a human or other mammal. Specifically, this invention is directed to a method of inhibiting TNNI3K activity, comprising contacting the kinase with an effective amount of a compound of Formula I or a pharmaceutically acceptable salt thereof. For example, TNNI3K activity may be inhibited in mammalian cardiac tissue by administering to a patient in need thereof, an effective amount a compound of Formula I or a pharmaceutically acceptable salt thereof.
The compounds of this invention may be particularly useful for treatment of TNNI3K-mediated diseases or disorders, specifically by inhibition of TNNI3K activity, where such diseases or disorders are selected from heart failure, particularly congestive heart failure; cardiac hypertrophy; and heart failure or congestive heart failure resulting from cardiac hypertrophy. The compounds of this invention may also be useful for the treatment of heart failure or congestive heart failure resulting from myocardial ischemia or myocardial infarction.
A therapeutically “effective amount” is intended to mean that amount of a compound that, when administered to a patient in need of such treatment, is sufficient to effect treatment, as defined herein. Thus, e.g., a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof, is a quantity of an inventive agent that, when administered to a human in need thereof, is sufficient to modulate or inhibit the activity of TNNI3K such that a disease condition which is mediated by that activity is reduced, alleviated or prevented. The amount of a given compound that will correspond to such an amount will vary depending upon factors such as the particular compound (e.g., the potency (pXC50), efficacy (EC50), and the biological half-life of the particular compound), disease condition and its severity, the identity (e.g., age, size and weight) of the patient in need of treatment, but can nevertheless be routinely determined by one skilled in the art. Likewise, the duration of treatment and the time period of administration (time period between dosages and the timing of the dosages, e.g., before/with/after meals) of the compound will vary according to the identity of the mammal in need of treatment (e.g., weight), the particular compound and its properties (e.g., pharmaceutical characteristics), disease or condition and its severity and the specific composition and method being used, but can nevertheless be determined by one of skill in the art.
“Treating” or “treatment” is intended to mean at least the mitigation of a disease condition in a patient, where the disease condition is caused or mediated by TNNI3K. The methods of treatment for mitigation of a disease condition include the use of the compounds in this invention in any conventionally acceptable manner, for example for prevention, retardation, prophylaxis, therapy or cure of a disease. The compounds of Formula I of this invention may be useful for the treatment of heart failure, particularly congestive heart failure. The compounds of Formula I of this invention may be useful for the treatment of cardiac hypertrophy, and heart failure or congestive heart failure resulting from cardiac hypertrophy, myocardial ischemia or myocardial infarction.
The compounds of the invention may be administered by any suitable route of administration, including both systemic administration and topical administration. Systemic administration includes oral administration, parenteral administration, transdermal administration, rectal administration, and administration by inhalation. Parenteral administration refers to routes of administration other than enteral, transdermal, or by inhalation, and is typically by injection or infusion. Parenteral administration includes intravenous, intramuscular, and subcutaneous injection or infusion. Inhalation refers to administration into the patient's lungs whether inhaled through the mouth or through the nasal passages. Topical administration includes application to the skin.
The compounds of the invention may be administered once or according to a dosing regimen wherein a number of doses are administered at varying intervals of time for a given period of time. For example, doses may be administered one, two, three, or four times per day. Doses may be administered until the desired therapeutic effect is achieved or indefinitely to maintain the desired therapeutic effect. Suitable dosing regimens for a compound of the invention depend on the pharmacokinetic properties of that compound, such as absorption, distribution, and half-life, which can be determined by the skilled artisan. In addition, suitable dosing regimens, including the duration such regimens are administered, for a compound of the invention depend on the condition being treated, the severity of the condition being treated, the age and physical condition of the patient being treated, the medical history of the patient to be treated, the nature of concurrent therapy, the desired therapeutic effect, and like factors within the knowledge and expertise of the skilled artisan. It will be further understood by such skilled artisans that suitable dosing regimens may require adjustment given an individual patient's response to the dosing regimen or over time as individual patient needs change.
Treatment of TNNI3K-mediated disease conditions may be achieved using the compounds of this invention as a monotherapy, or in dual or multiple combination therapy, such as in combination with other cardiovascular agents, for example, in combination with one or more of the following agents: a beta-blocker, an ACE inhibitor, an angiotensin receptor blocker (ARB), a calcium channel blocker, a diuretic, a renin inhibitor, a centrally acting antihypertensive, a dual ACE/NEP inhibitor, an aldosterone synthase inhibitor, and an aldosterone-receptor antagonist, which are administered in effective amounts as is known in the art.
Examples of suitable beta blockers include timolol (such as BLOCARDENTM) carteolol (such as CARTROLTM), carvedilol (such as COREGTM), nadolol (such as CORGARDTM), propanolol (such as INNOPRAN XLTM), betaxolol (such as KERLONETM) penbutolol (such as LEVATOLTM), metoprolol (such as LOPRESSORTM and TOPROL-XLTM), atenolol (such as TENORMINTM), pindolol (such as VISKENTM), bisoprolol, bucindolol, esmolol, acebutolol, labetalol, nebivolol, celiprolol, sotalol, and oxprenolol. Examples of suitable ACE inhibitors include alacepril, benazepril, benazaprilat, captopril, ceronapril, cilazapril, delapril, enalapril, enalaprilat, fosinopril, lisinopril, moexipiril, moveltopril, perindopril, quinapril, quinaprilat, ramipril, ramiprilat, spirapril, temocapril, trandolapril, and zofenopril. Preferred ACE inhibitors are benazepril, enalpril, lisinopril, and ramipril. Examples of suitable angiotensin receptor blockers include candesartan, eprosartan, irbesartan, losartan, olmesartan, tasosartan, telmisartan, and valsartan. Examples of suitable calcium channel blockers include dihydropyridines (DHPs) and non-DHPs. Suitable DHPs include amlodipine, felodipine, ryosidine, isradipine, lacidipine, nicardipine, nifedipine, nigulpidine, niludipine, nimodiphine, nisoldipine, nitrendipine, and nivaldipine, and their pharmaceutically acceptable salts. Suitable non-DHPs are flunarizine, prenylamine, diltiazem, fendiline, gallopamil, mibefradil, anipamil, tiapamil, and verampimil, and their pharmaceutically acceptable salts. A suitable diuretic is a thiazide derivative selected from amiloride, chlorothiazide, hydrochlorothiazide, methylchlorothiazide, and chlorothalidon. A suitable renin inhibitor is aliskiren. Examples of suitable centrally acting antihypertensives include clonidine, guanabenz, guanfacine and methyldopa. Examples of suitable dual ACE/NEP inhibitors include omapatrilat, fasidotril, and fasidotrilat. Examples of suitable aldosterone synthase inhibitors include anastrozole, fadrozole, and exemestane. Examples of suitable aldosterone-receptor antagonists include spironolactone and eplerenone.
The invention further includes the use of compounds of the invention as an active therapeutic substance, in particular in the treatment of diseases mediated by TNNI3K. Specifically, the invention includes the use of compounds of the invention in the treatment of heart failure, particularly congestive heart failure; cardiac hypertrophy; heart failure or congestive heart failure resulting from cardiac hypertrophy; and heart failure or congestive heart failure resulting from myocardial ischemia or myocardial infarction.
In another aspect, the invention includes the use of compounds of the invention in the manufacture of a medicament for use in the treatment of the above disorders.
Compositions
The compounds of the invention will normally, but not necessarily, be formulated into a pharmaceutical composition prior to administration to a patient. Accordingly, in another aspect the invention is directed to pharmaceutical compositions comprising a compound of the invention and a pharmaceutically-acceptable excipient.
The pharmaceutical compositions of the invention may be prepared and packaged in bulk form wherein an effective amount of a compound of the invention can be extracted and then given to the patient such as with powders, syrups, and solutions for injection. Alternatively, the pharmaceutical compositions of the invention may be prepared and packaged in unit dosage form. For oral application, for example, one or more tablets or capsules may be administered. A dose of the pharmaceutical composition contains at least a therapeutically effective amount of a compound of this invention (i.e., a compound of Formula I or a salt, particularly a pharmaceutically acceptable salt, thereof). When prepared in unit dosage form, the pharmaceutical compositions may contain from 1 mg to 1000 mg of a compound of this invention.
The pharmaceutical compositions of the invention typically contain one compound of the invention. However, in certain embodiments, the pharmaceutical compositions of the invention contain more than one compound of the invention. In addition, the pharmaceutical compositions of the invention may optionally further comprise one or more additional pharmaceutically active compounds.
As used herein, “pharmaceutically-acceptable excipient” means a material, composition or vehicle involved in giving form or consistency to the composition. Each excipient must be compatible with the other ingredients of the pharmaceutical composition when commingled such that interactions which would substantially reduce the efficacy of the compound of the invention when administered to a patient and interactions which would result in pharmaceutical compositions that are not pharmaceutically-acceptable are avoided. In addition, each excipient must of course be of sufficiently high purity to render it pharmaceutically-acceptable.
The compounds of the invention and the pharmaceutically-acceptable excipient or excipients will typically be formulated into a dosage form adapted for administration to the patient by the desired route of administration. Conventional dosage forms include those adapted for (1) oral administration such as tablets, capsules, caplets, pills, troches, powders, syrups, elixirs, suspensions, solutions, emulsions, sachets, and cachets; (2) parenteral administration such as sterile solutions, suspensions, and powders for reconstitution; (3) transdermal administration such as transdermal patches; (4) rectal administration such as suppositories; (5) inhalation such as aerosols and solutions; and (6) topical administration such as creams, ointments, lotions, solutions, pastes, sprays, foams, and gels.
Suitable pharmaceutically-acceptable excipients will vary depending upon the particular dosage form chosen. In addition, suitable pharmaceutically-acceptable excipients may be chosen for a particular function that they may serve in the composition. For example, certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the production of uniform dosage forms. Certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the production of stable dosage forms. Certain pharmaceutically-acceptable excipients may be chosen for their ability to facilitate the carrying or transporting the compound or compounds of the invention once administered to the patient from one organ, or portion of the body, to another organ, or portion of the body. Certain pharmaceutically-acceptable excipients may be chosen for their ability to enhance patient compliance.
Suitable pharmaceutically-acceptable excipients include the following types of excipients: diluents, fillers, binders, disintegrants, lubricants, glidants, granulating agents, coating agents, wetting agents, solvents, co-solvents, suspending agents, emulsifiers, sweeteners, flavoring agents, flavor masking agents, coloring agents, anti-caking agents, humectants, chelating agents, plasticizers, viscosity increasing agents, antioxidants, preservatives, stabilizers, surfactants, and buffering agents. The skilled artisan will appreciate that certain pharmaceutically-acceptable excipients may serve more than one function and may serve alternative functions depending on how much of the excipient is present in the formulation and what other ingredients are present in the formulation.
Skilled artisans possess the knowledge and skill in the art to enable them to select suitable pharmaceutically-acceptable excipients in appropriate amounts for use in the invention. In addition, there are a number of resources that are available to the skilled artisan which describe pharmaceutically-acceptable excipients and may be useful in selecting suitable pharmaceutically-acceptable excipients. Examples include Remington's Pharmaceutical Sciences (Mack Publishing Company), The Handbook of Pharmaceutical Additives (Gower Publishing Limited), and The Handbook of Pharmaceutical Excipients (the American Pharmaceutical Association and the Pharmaceutical Press).
The pharmaceutical compositions of the invention are prepared using techniques and methods known to those skilled in the art. Some of the methods commonly used in the art are described in Remington's Pharmaceutical Sciences (Mack Publishing Company).
In one aspect, the invention is directed to a solid oral dosage form such as a tablet or capsule comprising an effective amount of a compound of the invention and a diluent or filler. Suitable diluents and fillers include lactose, sucrose, dextrose, mannitol, sorbitol, starch (e.g. corn starch, potato starch, and pre-gelatinized starch), cellulose and its derivatives (e.g. microcrystalline cellulose), calcium sulfate, and dibasic calcium phosphate. The oral solid dosage form may further comprise a binder. Suitable binders include starch (e.g. corn starch, potato starch, and pre-gelatinized starch), gelatin, acacia, sodium alginate, alginic acid, tragacanth, guar gum, povidone, and cellulose and its derivatives (e.g. microcrystalline cellulose). The oral solid dosage form may further comprise a disintegrant. Suitable disintegrants include crospovidone, sodium starch glycolate, croscarmelose, alginic acid, and sodium carboxymethyl cellulose. The oral solid dosage form may further comprise a lubricant. Suitable lubricants include stearic acid, magnesium stearate, calcium stearate, and talc.
EXAMPLES
The following examples illustrate the invention. These examples are not intended to limit the scope of the present invention, but rather to provide guidance to the skilled artisan to prepare and use the compounds, compositions, and methods of the present invention. While particular embodiments of the present invention are described, the skilled artisan will appreciate that various changes and modifications can be made without departing from the spirit and scope of the invention.
In the following experimental descriptions, the following abbreviations may be used:














Abbreviation
Meaning









aq
aqueous



brine
saturated aqueous sodium chloride



CH2Cl2
methylene chloride



CH3CN or MeCN
acetonitrile



CH3SNa
sodium methyl mercaptide



d
day



DMF
N,N-dimethylformamide



DMSO
dimethylsulfoxide



equiv
equivalents



Et
ethyl



Et3N or TEA
triethylamine



Et2O
diethyl ether



EtOAc
ethyl acetate



h, hr
hour



HCl
hydrochloric acid



i-Pr2NEt
N′,N′-diisopropylethylamine



LCMS
liquid chromatography-mass spectroscopy



Me
methyl



MeOH or CH3OH
methanol



MgSO4
magnesium sulfate



min
minute



MS
mass spectrum



μw
microwave



NaH
sodium hydride



NaHCO3
sodium bicarbonate



Na2SO4
sodium sulfate



NH4Cl
ammonium chloride



Pd/C
palladium on carbon



Ph
phenyl



rt
room temperature



satd
saturated



SPE
solid phase extraction



TFA
trifluoroacetic acid



THF
tetrahydrofuran



tR
retention time










Preparation 1
4-fluoro-N-methyl-3-nitrobenzenesulfonamide




Step 1. 4-fluoro-3-nitrobenzenesulfonyl chloride
1-Fluoro-2-nitrobenzene (50.0 g, 0.354 mol) was added to chlorosulfonic acid (91 g, 0.778 mol) at 65° C. The resulting mixture was then heated to 100° C. for 18 h. The mixture was cooled to room temperature, poured over ice and extracted with methylene chloride. The combined organic layers were then washed with NaHCO3, then brine, dried over MgSO4, filtered and concentrated in vacuo to afford 4-fluoro-3-nitrobenzenesulfonyl chloride (55.3 g, 65% yield) as a brown oil.
Step 2. 4-fluoro-N-methyl-3-nitrobenzenesulfonamide
To a solution of 4-fluoro-3-nitrobenzenesulfonyl chloride (43 g, 179.5 mmol) in THF (500 mL), was added triethylamine (150 mL, 1.08 mol). The mixture was cooled to −35° C. and methylamine hydrochloride (14.5 g, 215.4 mmol) in water was added dropwise. After 1 h, the mixture was warmed to room temperature and diluted with 1:1 water/ethyl acetate. The organic layer was separated and washed with saturated aqueous sodium bicarbonate, then brine, dried over MgSO4, filtered and concentrated in vacuo. The crude residue was purified via column chromatography (20% ethyl acetate/petroleum ether) to give 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (38 g, 90% yield) as a yellow solid.
Preparation 2
3-amino-N-methyl-4-[(1-methylethyl)oxy]benzenesulfonamide




Step 1. N-methyl-4-[(1-methylethyl)oxy]-3-nitrobenzenesulfonamide
NaH (0.440 g, 11 mmol) was added to 20 mL of isopropanol and the resulting mixture stirred at room temperature. After 30 minutes, 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (2.34 g, 10 mmol) was added. The reaction mixture was then stirred at room temperature overnight. The mixture was poured into EtOAc and water. The organic phase was separated, dried over Na2SO4, and concentrated in vacuo to give the crude product. Purification via column chromatography (1:1 petroleum ether/ethyl acetate) afforded N-methyl-4-[(1-methylethyl)oxy]-3-nitrobenzenesulfonamide (1.6 g, 58% yield) as a yellow solid. MS (m/z) 274.7 (M+H+)
Step 2. 3-amino-N-methyl-4-[(1-methylethyl)oxy]benzenesulfonamide
To a mixture of N-methyl-4-[(1-methylethyl)oxy]-3-nitrobenzenesulfonamide (1.6 g, 5.8 mmol) in ethanol (20 mL) under nitrogen, Pd/C (0.160 g) was added. The flask was then evacuated and recharged with hydrogen three times. The resulting mixture was allowed to stir under a hydrogen atmosphere overnight at room temperature. The mixture was then filtered and concentrated to afford 3-amino-N-methyl-4-[(1-methylethyl)oxy]benzenesulfonamide (1.1 g, 77%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ 7.01-7.10 (m, 2H), 6.87-6.98 (m, 2H), 5.08 (br. s., 2H), 4.63 (dt, J=5.93, 11.98 Hz, 1H), 2.34-2.41 (m, 3H), 1.29 (d, J=6.02 Hz, 6H); MS (m/z) 244.7 (M+H+)
The following anilines were prepared from 4-fluoro-N-methyl-3-nitrobenzenesulfonamide using procedures analogous to those described in Preparation 2:














Conditions for
MS



Aniline Product
Step 1
(m/z)
1H NMR







3-amino-N-methyl-4-
sodium
217.0 (M + H+)
1H NMR (400 MHz, DMSO-


(methyloxy)benzenesulfonamide
methoxide,

d6) δ 7.09 (q, J = 4.85 Hz,



methanol

1H), 7.03 (s, 1H), 6.94 (s,





2H), 5.18 (s, 2H), 3.83 (s,





3H), 2.36 (d, J = 5.02 Hz,





3H)


3-amino-N-methyl-4-[(2,2,2-
NaH, 2,2,2-
285.0 (M + H+)
1H NMR (400 MHz, DMSO-


trifluoroethyl)oxy]benzenesulfonamide
trifluoroethanol

d6) δ ppm 7.18 (q, J = 5.02 Hz,





1 H), 7.05-7.13 (m, 2





H), 6.93 (dd, J = 8.53, 2.26 Hz,





1 H), 5.25 (s, 2 H),





4.81 (q, J = 8.87 Hz, 2 H), 2.38 (d,





J = 4.77 Hz, 3 H)


3-amino-N-methyl-4-[(2,2,2-
NaH, 1,1,1-
299.0 (M + H+)
1H NMR (400 MHz, DMSO-


trifluoro-1-
trifluoro-2-

d6) δ ppm 7.14-7.22 (m, 2


methylethyl)oxy]benzenesulfonamide
propanol

H), 7.11 (d, J = 2.26 Hz, 1 H),





6.92 (dd, J = 8.41, 2.38 Hz, 1





H), 5.19-5.30 (m, 3 H),





2.39 (d, J = 5.02 Hz, 3 H),





1.45 (d, J = 6.27 Hz, 3 H)









Preparation 3
3-amino-N-methyl-4-(methylthio)benzenesulfonamide




Step 1. N-methyl-4-(methylthio)-3-nitrobenzenesulfonamide
To a solution of 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (15 g, 64.01 mmol) in THF (150 mL), was added 20% CH3SNa (22.4 g, 64.01 mmol) dropwise. The resulting mixture was then stirred overnight. In the morning, the mixture was poured into ethyl acetate and water, the organic phase separated, dried over Na2SO4, filtered and concentrated. The crude material was then purified via column chromatography (1:1 ethyl acetate/petroleum ether) to afford N-methyl-4-(methylthio)-3-nitrobenzenesulfonamide (3.29 g, 19%) as a yellow solid. MS (m/z) 262.7 (M+H+)
Step 2. 3-amino-N-methyl-4-(methylthio)benzenesulfonamide
To a solution of N-methyl-4-(methylthio)-3-nitrobenzenesulfonamide (1.0 g, 3.81 mmol) in 10 mL of ethanol and 10 mL of NH4Cl, zinc dust (2.5 g, 3.81 mmol) was added. The reaction mixture was stirred overnight at room temperature. The mixture was then filtered and diluted with ethyl acetate and water. The organic phase was separated, washed with water and brine, dried over MgSO4, filtered and concentrated to afford 3-amino-N-methyl-4-(methylthio)benzenesulfonamide (0.500 g, 56%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ 7.06 (d, J=8.03 Hz, 1H), 6.86 (s, 1H), 6.67-6.76 (m, 1H), 5.28 (br. s., 2H), 2.17 (s, 3H), 2.21 (s, 3H); MS (m/z) 232.7 (M+H+)
The following anilines were prepared from 4-fluoro-N-methyl-3-nitrobenzenesulfonamide using procedures analogous to those described in Preparation 3:













Aniline Product
Conditions
MS (m/z)
1H NMR







3-amino-N-methyl-4-[(2,2,2-
Step 1: 2,2,2-
300.7
1H NMR (400 MHz, DMSO-


trifluoroethyl)thio]benzene-
trifluoroethane
(M + H+)
d6) δ ppm 7.48 (d, J = 7.94


sulfonamide
thiol, NaH

Hz, 1 H), 7.33 (q, J = 5.00 Hz,



Step 2: NiCl2,

1 H), 7.14 (d, J = 1.98 Hz, 1



NaBH4, MeOH

H), 6.85 (dd, J = 8.05, 2.09





Hz, 1 H), 5.87 (s, 2 H), 3.72





(q, J = 10.36 Hz, 2 H), 2.39





(d, J = 5.07 Hz, 3 H)









Preparation 4
3-amino-N-methyl-4-(4-morpholinyl)benzenesulfonamide




Step 1. N-methyl-4-(4-morpholinyl)-3-nitrobenzenesulfonamide
To a solution of 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (2.00 g, 8.54 mmol) and morpholine (0.744 g, 8.54 mmol) in THF (100 mL), was added N,N-diisopropylethylamine (2.21 g, 17.08 mmol). The resulting solution was stirred at 50° C. overnight. In the morning, the reaction mixture was cooled to room temperature and concentrated to dryness in vacuo. The residue was dissolved in ethyl acetate and washed with water and brine, dried over MgSO4, filtered and concentrated in vacuo to obtain N-methyl-4-(4-morpholinyl)-3-nitrobenzenesulfonamide (2.5 g, 97%) as a red oil. MS (m/z) 302.0 (M+H+)
Step 2. 3-amino-N-methyl-4-(4-morpholinyl)benzenesulfonamide
To a mixture of N-methyl-4-(4-morpholinyl)-3-nitrobenzenesulfonamide (2.5 g, 8.30 mmol) in THF (100 mL) under nitrogen, Pd/C (0.8 g) was added. The flask was then evacuated and recharged with hydrogen three times. The resulting mixture was allowed to stir under a hydrogen atmosphere at 50° C. overnight. The mixture was then filtered and concentrated to afford 3-amino-N-methyl-4-(4-morpholinyl)benzenesulfonamide (1.98 g, 88%). 1H NMR (400 MHz, DMSO-d6) δ 7.07-7.17 (m, 2H), 7.01 (d, J=8.28 Hz, 1H), 6.94 (dd, J=1.88, 8.16 Hz, 1H), 5.20 (s, 2H), 3.72-3.81 (m, 4H), 2.80-2.89 (m, 4H), 2.38 (d, J=4.77 Hz, 3H); MS (m/z) 272.2 (M+H+)
The following anilines were prepared from 4-fluoro-N-methyl-3-nitrobenzenesulfonamide and the indicated amine using the procedures described in Preparation 4:














Amine/





Conditions in


Aniline Product
Step 1
MS (m/z)
1H NMR







3-amino-4-
dimethyl amine
230.2
1H NMR (400 MHz, DMSO-d6) δ 7.03-


(dimethylamino)-N-

(M + H+)
7.10 (m, 2H), 7.00 (d, J = 8.28 Hz,


methylbenzenesulfon-


1H), 6.93 (dd, J = 2.13, 8.16 Hz, 1H),


amide


5.13 (s, 2H), 2.62 (s, 6H), 2.38 (d, J =





5.02 Hz, 3H)


3-amino-4-
ethyl(methyl)
244.1
1H NMR (400 MHz, DMSO-d6) δ 7.06-


[ethyl(methyl)amino]-
amine
(M + H+)
7.13 (m, 2H), 7.02 (d, J = 8.28 Hz,


N-methylbenzenesulfon-


1H), 6.93 (dd, J = 1.76, 8.03 Hz, 1H),


amide


5.11 (s, 2H), 2.89 (q, J = 7.03 Hz,





2H), 2.60 (s, 3H), 2.39 (d, J = 5.02





Hz, 3H), 1.03 (t, J = 7.03 Hz, 3H)


3-amino-4-(4,4-
4,4-difluoro-
306.1
1H NMR (400 MHz, DMSO-d6) δ ppm


difluoro-1-
piperidine
(M + H+)
7.15 (br. s., 1 H), 7.10 (d, J = 2.01 Hz,


piperidinyl)-N-


1 H), 7.05 (d, J = 8.28 Hz, 1 H), 6.92


methylbenzenesulfon-


(dd, J = 8.16, 2.13 Hz, 1 H), 5.29 (s, 2


amide


H), 2.95 (t, J = 4.77 Hz, 4 H), 2.39 (s, 3





H), 2.10-2.25 (m, 4 H)


3-amino-N-methyl-4-
(2R)-2-
324.1
1H NMR (400 MHz, DMSO-d6) δ ppm


[(2R)-2-(trifluoro-
(trifluoromethyl)
(M + H+)
7.31 (d, J = 8.28 Hz, 1 H), 7.13-7.22


methyl)-1-pyrrolidinyl]ben-
pyrrolidine

(m, 1 H), 7.10 (d, J = 2.26 Hz, 1 H),


zenesulfonamide
(conditions:

6.91 (dd, J = 8.28, 2.26 Hz, 1 H), 5.20



dioxane, 110° C.,

(s, 2 H), 4.40-4.53 (m, 1 H), 3.49-



sealed tube)

3.59 (m, 1 H), 2.64-2.78 (m, 1 H),





2.42 (s, 3 H), 2.27-2.37 (m, 1 H),





1.84-2.04 (m, 3 H)


3-amino-N-methyl-4-
piperidine
270.1
1H NMR (400 MHz, DMSO-d6) δ ppm


(1-piperidinyl)benzene-

(M + H+)
7.11 (br. s., 1 H), 7.08 (d, J = 2.01 Hz,


sulfonamide


1 H), 6.99 (d, J = 8.28 Hz, 1 H), 6.91-





6.96 (m, 1 H), 5.09 (s, 2 H), 2.74-





2.86 (m, 4 H), 2.38 (s, 3 H), 1.64-





1.72 (m, 4H), 1.48-1.58 (m, 2 H)


3-amino-4-(2,5-
2,5-dimethylpyrrolidine
284.1
1H NMR (400 MHz, DMSO-d6) δ ppm


dimethyl-1-

(M + H+)
7.29 (d, J = 8.03 Hz, 1 H), 7.19 (br. s.,


pyrrolidinyl)-N-


1 H), 7.09 (d, J = 2.26 Hz, 1 H), 6.92


methylbenzene-


(dd, J = 8.16, 2.13 Hz, 1 H), 5.38 (s, 2


sulfonamide


H), 3.04-3.14 (m, 2 H), 2.41 (s, 3 H),





1.95-2.07 (m, 2 H), 1.42-1.55 (m, 2





H), 0.88 (d, J = 6.02 Hz, 6 H)









Preparation 5
3-amino-4-fluoro-N-methylbenzenesulfonamide




To a mixture of 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (1.6 g, 6.83 mmol) in THF (50 mL) under nitrogen, Pd/C (0.600 g) was added. The flask was then evacuated and recharged with hydrogen. The resulting mixture was allowed to stir under a hydrogen atmosphere overnight at 50° C. The mixture was then filtered and concentrated to afford 3-amino-4-fluoro-N-methylbenzenesulfonamide (1.25 g, 89%) as an off-white solid. 1H NMR (400 MHz, DMSO-d6) δ 7.26 (q, J=4.85 Hz, 1H), 7.13-7.22 (m, 2H), 6.90 (ddd, J=2.38, 4.27, 8.41 Hz, 1H), 5.63 (s, 2H), 2.40 (d, J=5.02 Hz, 3H); MS (m/z) 205.1 (M+H+)
Preparation 6
3-amino-4-chloro-N-methylbenzenesulfonamide




Step 1. 4-chloro-N-methyl-3-nitrobenzenesulfonamide
A solution of 4-chloro-3-nitrobenzenesulfonyl chloride (10 g, 39.1 mmol) in THF (100 mL) was cooled to −40° C. before being treated with a solution of methylamine hydrochloride (2.64 g, 39.1 mmol) in 10 ml of water followed by TEA (5.44 mL, 39.1 mmol). The reaction mixture was stirred and allowed to warm to room temperature over 1 hour before being partitioned between 350 mL EtOAc and 30 mL brine. The organic layer was washed twice with brine, dried over MgSO4 and subjected to flash chromatography (330 g silica gel, 0-40% EtOAc/hexane) to afford 4-chloro-N-methyl-3-nitrobenzenesulfonamide (6.38 g, 65%) as a light yellow solid. MS (m/z) 251.0 (M+H+).
Step 2. 3-amino-4-chloro-N-methylbenzenesulfonamide
A solution of 4-chloro-N-methyl-3-nitrobenzenesulfonamide (6.35 g, 25.3 mmol) in EtOH (150 mL) and water (50.0 mL) was treated with iron (14.15 g, 253 mmol) and NH4Cl (13.55 g, 253 mmol) and heated at 90° C. for 4 hours before being cooled and filtered through celite. The filter cake was washed with EtOAc and the combined filtrate was filtered again to remove precipitated NH4Cl before being concentrated. The resulting crude material was partitioned between 350 ml EtOAc and 50 ml saturated aqueous NaHCO3. The organic layer was washed with brine, dried over MgSO4, concentrated and subjected to flash column chromatography (330 g silica gel, 0-15% EtOAc/DCM) to afford 3-amino-4-chloro-N-methylbenzenesulfonamide (5.60 g, 100%) as a light yellow crystalline solid. 1H NMR (400 MHz, MeOD) δ ppm 7.39 (d, J=8.28 Hz, 1 H), 7.27 (d, J=2.26 Hz, 1 H), 7.03 (dd, J=8.28, 2.26 Hz, 1 H), 2.54 (s, 3 H). MS 221.0 (M+H+).
Preparation 7
3-amino-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide




Step 1. phenylmethyl[(4-fluoro-3-nitrophenyl)sulfonyl]methylcarbamate
A solution of 4-fluoro-N-methyl-3-nitrobenzenesulfonamide (2 g, 8.54 mmol) in THF (20 mL) was treated with triethylamine (2.380 mL, 17.08 mmol) followed by dropwise addition of benzyl chloroformate (3.75 mL, 11.10 mmol). The mixture was stirred at 25° C. for 5 hours before being concentrated. The residue was treated with water and extracted with CH2Cl2. The organic extracts were washed (brine), dried (sodium sulfate), concentrated, and subjected to flash chromatography (25-50% EtOAc-hexanes) to give a yellow solid, which was suspended in EtOAc-hexanes, collected by filtration, and washed with hexanes to give phenylmethyl[(4-fluoro-3-nitrophenyl)sulfonyl]methylcarbamate (1 g, 32%) as a white solid. MS (m/z) 391.0 (M+Na+).
Step 2. phenylmethyl methyl({3-nitro-4-[(2,2,2-trifluoroethyl)amino]phenyl}sulfonyl)carbamate
A solution of phenylmethyl[(4-fluoro-3-nitrophenyl)sulfonyl]methylcarbamate (1 g, 2.71 mmol) in THF (10 mL) at 25° C. was treated with 2,2,2-trifluoroethylamine (0.592 g, 5.97 mmol) and stirred for 20 hours before being concentrated to give a yellow oil, which was dissolved in EtOAc/hexanes. A yellow precipitate formed, which was collected by filtration and washed with hexanes to give phenylmethyl methyl({3-nitro-4-[(2,2,2-trifluoroethyl)amino]phenyl}sulfonyl)carbamate (1.07 g, 88%) as a yellow solid. MS (m/z) 448.1 (M+H+).
Step 3. phenylmethyl methyl({4-[methyl(2,2,2-trifluoroethyl)amino]-3-nitrophenyl}sulfonyl)carbamate
A solution of phenylmethyl methyl({3-nitro-4-[(2,2,2-trifluoroethyl)amino]phenyl}sulfonyl)carbamate (1 g, 2.24 mmol) in DMF (1 mL) at 25° C. was treated with sodium hydride (0.179 g, 4.47 mmol) and stirred for 2 minutes before being treated with iodomethane (0.42 mL, 6.71 mmol). After 1 hour, the mixture was diluted with water and extracted with EtOAc. The organic extract was washed (brine), dried (sodium sulfate), concentrated, and subjected to flash chromatography (10-35% EtOAc-hexanes) to give phenylmethyl methyl({4-[methyl(2,2,2-trifluoroethyl)amino]-3-nitrophenyl}sulfonyl) carbamate (539 mg, 52%) as a yellow oil. MS (m/z) 462.1 (M+H+).
Step 4. 3-amino-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide
A solution of phenylmethyl methyl({4-[methyl(2,2,2-trifluoroethyl)amino]-3-nitrophenyl}sulfonyl)carbamate (539 mg, 1.17 mmol) in MeOH (10 mL) at 25° C. was treated with 10% palladium on carbon (124 mg, 0.117 mmol) and stirred under an atmosphere of hydrogen (balloon) overnight before being filtered through celite. The filtrate was again filtered through a 0.45 micron syringe filter and concentrated to give 3-amino-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide (320 mg, 92%) as a brown oil. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.14-7.20 (m, 2 H), 7.12 (d, J=2.26 Hz, 1 H), 6.95 (dd, J=8.28, 2.26 Hz, 1 H), 5.23 (s, 2 H), 3.82 (q, J=9.87 Hz, 2 H), 2.83 (s, 3 H), 2.39 (d, J=5.02 Hz, 3 H). MS (m/z) 298.0 (M+H+).
Preparation 8
5-amino-2-fluoro-N-methylbenzenesulfonamide




Step 1. 2-fluoro-5-nitrobenzenesulfonyl chloride
A mixture of 1-fluoro-4-nitrobenzene (3.0 g, 21.3 mmol) in chlorosulfonic acid (5.5 mL, 84 mmol) was stirred at 90-100° C. for 8 hours before being cooled to room temperature and slowly poured into ice water and extracted with EtOAc. The organic extract was washed with saturated aq. NaHCO3 and water, dried (sodium sulfate), and concentrated to give 2-fluoro-5-nitrobenzenesulfonyl chloride (3.2 g, 63%) as a colorless oil, which was used directly in the next step.
Step 2. 2-fluoro-N-methyl-5-nitrobenzenesulfonamide
A solution of 2-fluoro-5-nitrobenzenesulfonyl chloride (3.2 g, 12.6 mmol) in THF (30 mL) at −45° C. was treated with methylamine hydrochloride (1.0 g, 15.1 mmol) and triethylamine (2.1 mL, 15.1 mmol) and stirred for 30 minutes. The mixture was then treated with 6M aqueous HCl to adjust the pH to 3 and warmed to room temperature before being diluted with water and extracted with EtOAc. The organic extract was dried (sodium sulfate), concentrated, and subjected to flash chromatography (5-20% EtOAc-petroleum ether) to give 2-fluoro-N-methyl-5-nitrobenzenesulfonamide as a yellow solid (3.0 g, 93%). MS (m/z) 235.1 (M+H+).
Step 3. 5-amino-2-fluoro-N-methylbenzenesulfonamide
A solution of 2-fluoro-N-methyl-5-nitrobenzenesulfonamide (3.0 g, 12.8 mmol) in
MeOH (40 mL) was treated with 10% palladium on carbon (300 mg, 0.28 mmol) and stirred under hydrogen (40 psi) for 8 hours before being filtered through celite and concentrated to give 5-amino-2-fluoro-N-methylbenzenesulfonamide (2.5 g, 96%) as an off-white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.40-7.49 (m, 1 H), 7.01-7.09 (m, 1 H), 6.94 (dd, J=5.95, 2.87 Hz, 1 H), 6.71-6.77 (m, 1 H), 5.49 (br. s., 2 H), 2.45 (d, J=4.85 Hz, 3 H). MS (m/z) 205.1 (M+H+).
The following anilines were prepared from the indicated nitrobenzenes using procedures analogous to those described in Preparation 8:














Nitrobenzene




Aniline Product
in Step 1
MS (m/z)
1H NMR







3-amino-N-methyl-4-
1-nitro-2-
271.0
1H NMR (400 MHz, DMSO-d6) δ ppm


[(trifluoromethyl)oxy]ben-
[(trifluorometh-
(M + H+)
7.39 (q, J = 4.77 Hz, 1 H), 7.31 (dd,


zenesulfonamide
yl)oxy]benzene

J = 8.53, 1.51 Hz, 1 H), 7.24 (d, J = 2.26





Hz, 1 H), 6.92 (dd, J = 8.41, 2.38 Hz, 1 H),





5.92 (s, 2 H), 2.43 (d, J = 4.77 Hz, 3 H)


5-amino-2-fluoro-N-
4-fluoro-2-
235.1
1H NMR (400 MHz, DMSO-d6) δ ppm


methyl-4-
(methyloxy)-1-
(M + H+)
7.31 (br. s., 1 H), 6.96 (d, J = 7.28 Hz, 1


(methyloxy)benzene-
nitrobenzene

H), 6.90 (d, J = 11.91 Hz, 1 H), 4.97 (s, 2


sulfonamide


H), 3.82 (s, 3 H), 2.40 (d, J = 3.75 Hz, 3 H)









Preparation 9
5-amino-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide




Step 1. 2,4-difluoro-5-nitrobenzenesulfonyl chloride
A mixture of 2,4-difluoro-1-nitrobenzene (20 g, 126 mmol) in chlorosulfonic acid (44 g, 378 mmol) was stirred at 100° C. for 48 hours before being poured into ice-water and extracted with EtOAc. The organic extract was dried (Na2SO4) and concentrated, and the residue was triturated with 10% EtOAc-petroleum ether to give 2,4-difluoro-5-nitrobenzenesulfonyl chloride as a brown oil (21 g, 81%) which was used directly in the next step.
Step 2. 2,4-difluoro-N-methyl-5-nitrobenzenesulfonamide
A solution of 2,4-difluoro-5-nitrobenzenesulfonyl chloride (21 g, 81 mmol) in THF (400 mL) at −60° C. was treated with methylamine hydrochloride (6.6 g, 97 mmol) and then treated dropwise with triethylamine (22.6 mL, 162 mmol). After stirring for 6 hours at −60 to −40° C. the mixture was adjusted to pH 3 with the addition of 15% aqueous HCl, diluted with water, and extracted with EtOAc. The organic extracts were dried (Na2SO4), concentrated, and subjected to flash chromatography (17% EtOAc-petroleum ether) to give 2,4-difluoro-N-methyl-5-nitrobenzenesulfonamide (8 g, 38%) as a brown solid. 1H NMR (400 MHz, CDCl3) δ ppm 8.66-8.74 (m, 1 H), 7.20-7.25 (m, 1 H), 4.81-4.91 (m, 1 H), 2.78-2.81 (m, 3 H).
Step 3. 4-(dimethylamino)-2-fluoro-N-methyl-5-nitrobenzenesulfonamide
A solution of 2,4-difluoro-N-methyl-5-nitrobenzenesulfonamide (8.0 g, 31.6 mmol) in CH2Cl2 (200 mL) at −20° C. was treated with dimethylamine hydrochloride (2.56 g, 31.6 mmol). The resulting mixture was treated dropwise with triethylamine and stirred for 1 hour before being treated with 15% aqueous HCl to adjust the pH to 3, diluted with water, and extracted with EtOAc. The organic extract was dried (sodium sulfate), concentrated, and subjected to flash chromatography (20-50% EtOAc-petroleum ether) to give 4-(dimethylamino)-2-fluoro-N-methyl-5-nitrobenzenesulfonamide (4.0 g, 46%) as a yellow solid. MS (m/z) 278.1 (M+H+).
Step 4. 5-amino-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide
A solution of 4-(dimethylamino)-2-fluoro-N-methyl-5-nitrobenzenesulfonamide (4.0 g, 14.3 mmol) in MeOH (100 mL) was treated with 10% Pd/C (400 mg) and stirred under H2 (50 psi) for 16 hours before being filtered, concentrated, and subjected to flash chromatography (33-50% EtOAc-petroleum ether) to give 5-amino-4-(d imethylamino)-2-fluoro-N-methylbenzenesulfonamide as a white solid (2.5 g, 71%). 1H NMR (400 MHz, CDCl3) δ ppm 7.13 (d, J=7.28 Hz, 1 H), 6.75 (d, J=11.69 Hz, 1 H), 4.58 (q, J=4.85 Hz, 1 H), 3.87 (br. s., 2 H), 2.66 (d, J=5.51 Hz, 3 H). MS (m/z) 248.1 (M+H+).
Preparation 10
5-amino-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide




Step 1. 4-fluoro-1-nitro-2-[(2,2,2-trifluoroethyl)oxy]benzene
A mixture of 2,4-difluoro-1-nitrobenzene (10 g, 62.9 mmol) and 2,2,2-trifluoroethanol (6.29 g, 62.9 mmol) in THF (100 mL) at 25° C. was treated with Cs2CO3 (20.5 g, 62.9 mmol) and stirred for 8 hours before being diluted with the addition of water and extracted with EtOAc. The organic extract was dried (Na2SO4), concentrated, and subjected to flash chromatography (3% EtOAc-petroleum ether) to give 4-fluoro-1-nitro-2-[(2,2,2-trifluoroethyl)oxy]benzene (10 g, 67%) as a yellow solid. MS (m/z) 240.0 (M+H+).
Step 2. 2-fluoro-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonyl chloride
A mixture of 4-fluoro-1-nitro-2-[(2,2,2-trifluoroethyl)oxy]benzene (10 g, 41.8 mmol) in chlorosulfonic acid (82 mL, 125.5 mmol) was stirred at 50° C. for 8 hours before being poured into ice and extracted with EtOAc. The organic extracts were dried (Na2SO4) and concentrated to give 2-fluoro-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonyl chloride (15 g, crude) as a brown oil, which was used directly in the next step.
Step 3. 2-fluoro-N-methyl-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide
A mixture of 2-fluoro-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonyl chloride (15 g, crude) in THF (150 mL) at −45° C. was treated with methylamine hydrochloride (5.96 g, 89 mmol) and then treated dropwise with triethylamine (12.4 mL, 89 mmol). After stirring for 1 hour at −45° C., the mixture was adjusted to pH 3 by the addition of aqueous 3M HCl, warmed to room temperature, diluted with water, and extracted with EtOAc. The organic extract was dried (Na2SO4), concentrated, and subjected to flash chromatography (9-17% EtOAc-petroleum ether) to give 2-fluoro-N-methyl-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (10 g, 72% for two steps) as a yellow solid. MS (m/z) 333.0 (M+H+).
Step 4. 5-amino-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide
A mixture of 2-fluoro-N-methyl-5-nitro-4-[(2,2,2-trifluoroethyl)oxy]benzene-sulfonamide (10 g, 30.1 mmol) in MeOH (150 mL) was treated with 10% Pd/C (1 g) and stirred under H2 (45 psi) at 45° C. for 10 hours before being filtered. The filtrate was concentrated to give 5-amino-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzene-sulfonamide (8 g, 88%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.40 (q, J=5.07 Hz, 1 H), 7.10 (d, J=11.69 Hz, 1 H), 7.05 (d, J=7.28 Hz, 1 H), 5.04 (s, 2 H), 4.83 (q, J=8.82 Hz, 2 H), 2.42 (d, J=4.41 Hz, 3 H). MS (m/z) 303.0 (M+H+).
Preparation 11
3-amino-N-methyl-4-(methylsulfonyl)benzenesulfonamide




Step 1. N-methyl-4-(methylsulfonyl)-3-nitrobenzenesulfonamide
A mixture of N-methyl-4-(methylthio)-3-nitrobenzenesulfonamide (1.0 g, 3.81 mmol) in AcOH (15 mL) was treated dropwise with 30% aqueous H2O2 (5 mL, 44 mmol) and stirred at 117° C. for 2 hours before being cooled to room temperature and diluted with water (100 mL). The precipitate that formed was collected by filtration and dried to give N-methyl-4-(methylsulfonyl)-3-nitrobenzenesulfonamide (1.5 g, crude) as a yellow solid, which was used directly in the next step.
Step 2. 3-amino-N-methyl-4-(methylsulfonyl)benzenesulfonamide
A solution of N-methyl-4-(methylsulfonyl)-3-nitrobenzenesulfonamide (1.5 g, crude) in EtOH (20 mL) and saturated aqueous NH4Cl (20 mL) was treated with Zn dust (3.3. g, 51 mmol) and stirred at 50° C. overnight before being filtered (EtOAc wash). The filtrate was diluted with water and extracted with EtOAc. The organic extract was washed with water and brine, dried (MgSO4), concentrated, and subjected to flash chromatography (33% EtOAc-petroleum ether) to give 3-amino-N-methyl-4-(methylsulfonyl)benzenesulfonamide (950 mg, 94% 2 steps) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.73 (d, J=8.28 Hz, 1 H), 7.58 (q, J=4.94 Hz, 1 H), 7.34 (d, J=1.76 Hz, 1 H), 7.01 (dd, J=8.53, 1.76 Hz, 1 H), 6.48 (s, 2 H), 3.18 (s, 3 H), 2.47 (d, J=5.02 Hz, 3 H). MS (m/z) 265.0 (M+H+).
Preparation 12
4,5-dichloro-6,7-bis(methyloxy)quinazoline




A mixture of 5-chloro-6,7-bis(methyloxy)-4(1H)-quinazolinone (100 mg, 0.42 mmol, prepared according to J. Med. Chem. (1999) 42 3860) in POCl3 (2 mL) was heated at 80-90° C. for 8 hours before being concentrated. The residue was treated with saturated aqueous NaHCO3 and extracted with EtOAc. The organic extract was dried (Na2SO4) and concentrated to give 4,5-dichloro-6,7-bis(methyloxy)quinazoline (70 mg, 65%) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.94 (s, 1 H), 7.56 (s, 1 H), 4.05 (s, 3 H), 3.87 (s, 3 H). MS (m/z) 258.9 (M+H+).
The following 4-chloroquinazolines were prepared from the indicated commercially available or known quinazolinones using procedures analogous to those described in Preparation 12:













Quinazoline Product
Quinazolinone SM
MS (m/z)
1H NMR







4-chloro-6,7-bis{[2-
6,7-bis{[2-
313.0
1H NMR (400 MHz, DMSO-


(methyloxy)ethyl]oxy}quin-
(methyloxy)ethyl]oxy}-
(M + H+)
d6) δ ppm 8.88 (s, 1 H), 7.50


azoline
4(1H)-quinazolinone

(s, 1 H), 7.46 (s, 1 H), 4.32-





4.42 (m, 4 H), 3.75-3.81





(m, 4 H), 3.37 (s, 3 H), 3.36





(s, 3 H)


4-chloro-6-iodo-7-
6-iodo-7-
320.7
1H NMR (400 MHz, DMSO-


(methyloxy)quinazoline
(methyloxy)-4(1H)-
(M + H+)
d6) δ ppm 9.03 (s, 1 H), 8.67



quinazolinone

(s, 1 H), 7.48 (s, 1 H), 4.08



(prepared as in

(s, 3 H)



U.S. Pat. No. 6,225,318



B1 (2001))









Preparation 13
4-chloro-6-(ethyloxy)-7-(methyloxy)quinazoline




Step 1. 6-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone
A mixture of 6-hydroxy-7-(methyloxy)-4(1H)-quinazolinone (1 g, 5.2 mmol) in water (12 mL), MeCN (6 mL), and MeOH (1 mL) at 25° C. was treated with Cs2CO3 (1.7 g, 5.2 mmol). After the mixture was stirred for 30 minutes, iodoethane (0.42 mL, 5.2 mmol) was added and the mixture was stirred at 60° C. for 40 hours before being cooled to room temperature. The solid was collected by filtration and washed with MeOH to give 6-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone (560 mg, 49%). MS (m/z) 221.1 (M+H+).
Step 2. 4-chloro-6-(ethyloxy)-7-(methyloxy)quinazoline
A mixture of 6-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone (560 mg, 2.54 mmol) in POCl3 (5 mL, 53.6 mmol) was treated with 1 drop of DMF and heated at 100° C. for 2 hours before being concentrated. The residue was treated with saturated aqueous NaHCO3 and extracted with CH2Cl2. The organic extracts were dried (sodium sulfate) and concentrated to give 4-chloro-6-(ethyloxy)-7-(methyloxy)quinazoline (416 mg, 1.74 mmol, 69%) as an off-white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.88 (s, 1 H), 7.46 (s, 1 H), 7.39 (s, 1 H), 4.26 (q, J=6.94 Hz, 2 H), 4.02 (s, 3 H), 1.44 (t, J=7.03 Hz, 3 H). MS (m/z) 239.0 (M+H+).
The following quinazolines were prepared using procedures analogous to those described in Preparation 13 by substituting the indicated alkyl halide in the place iodoethane in Step 1:














Alkyl Halide in




Quinazoline Product
Step 1
MS (m/z)
1H NMR







4-chloro-6-[(1-methylethyl)oxy]-
2-bromopropane
253.0
1H NMR (400 MHz, DMSO-


7-(methyloxy)quinazoline

(M + H+)
d6) δ ppm 8.88 (s, 1 H), 7.46





(s, 1 H), 7.41 (s, 1 H), 4.86-





4.97 (m, 1 H), 4.01 (s, 3 H),





1.38 (d, J = 6.02 Hz, 6 H)









Preparation 14
4-chloro-5-(ethyloxy)-7-(methyloxy)quinazoline




Step 1. 5-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone
A mixture of [5-hydroxy-7-(methyloxy)-4-oxo-3(4H)-quinazolinyl]methyl 2,2-dimethylpropanoate (1 g, 3.26 mmol, prepared according to J. Med. Chem. (2006) 49 6465), triphenylphosphine (1.370 g, 5.22 mmol), and ethanol (0.210 mL, 3.59 mmol) in DCM (10 mL) at 0° C. was treated dropwise over 2 minutes with a 40% solution of DEAD in toluene (2.379 mL, 5.22 mmol). After addition, the mixture was warmed to room temperature and stirred for 3 hours before being concentrated. The residue was dissolved in 7 M ammonia in methanol (10 mL, 70.0 mmol) and the mixture was stirred for 3 days. The solid was collected by filtration and washed with MeOH and CH2Cl2 to give 5-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone (286 mg, 40%) as a white solid. MS (m/z) 221.1 (M+H+).
Step 2. 4-chloro-5-(ethyloxy)-7-(methyloxy)quinazoline
A mixture of 5-(ethyloxy)-7-(methyloxy)-4(1H)-quinazolinone (285 mg, 1.29 mmol) in POCl3 (10 mL, 107 mmol) was treated with 1 drop of DMF and stirred at 100° C. for 1 hour before being concentrated. The residue was treated with saturated aqueous NaHCO3 and extracted with CH2Cl2. The organic extracts were dried (sodium sulfate), concentrated, and subjected to flash chromatography (1-3% MeOH—CH2Cl2) to give 4-chloro-5-(ethyloxy)-7-(methyloxy)quinazoline (260 mg, 84%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.84 (s, 1 H), 7.03 (d, J=2.51 Hz, 1 H), 6.84 (d, J=2.26 Hz, 1 H), 4.22 (q, J=6.86 Hz, 2 H), 3.96 (s, 3 H), 1.47 (t, J=6.90 Hz, 3 H). MS (m/z) 239.0 (M+H+).
The following quinazolines were prepared using procedures analogous to those described in Preparation 14 by substituting the indicated alcohol in the place of ethanol in Step 1:














Alcohol in




Quinazoline Product
Step 1
MS (m/z)
1H NMR







4-chloro-5-[(1-
2-propanol
253.0
1H NMR (400 MHz, DMSO-d6) δ


methylethyl)oxy]-7-

(M + H+)
ppm 8.82 (s, 1 H), 7.02 (d,


(methyloxy)quinazoline


J = 2.26 Hz, 1 H), 6.88 (d,





J = 2.26 Hz, 1 H), 4.85-4.96 (m,





1 H), 3.96 (s, 3 H), 1.40 (d,





J = 6.02 Hz, 6 H)


4-chloro-7-(methyloxy)-5-
tetrahydro-2H-
295.0
1H NMR (400 MHz, DMSO-d6)


(tetrahydro-2H-pyran-4-
pyran-4-ol
(M + H+)
δ ppm 8.69 (br. s., 1 H), 6.78 (s,


yloxy)quinazoline


2 H), 4.81-4.91 (m, 1 H), 3.86-





3.93 (m, 5 H), 3.49-3.56 (m,





2 H), 1.89-2.01 (m, 2 H), 1.68





(m, J = 13.49, 6.93, 6.93, 3.51





Hz, 2 H)


4-chloro-7-(methyloxy)-5-{[2-
2-(methyloxy)
269.0
1H NMR (400 MHz, DMSO-d6) δ


(methyloxy)ethyl]oxy}quin-
ethanol
(M + H+)
ppm 8.84 (s, 1 H), 7.04 (d,


azoline


J = 2.26 Hz, 1 H), 6.88 (d,





J = 2.26 Hz, 1 H), 4.26-4.33 (m,





2 H), 3.96 (s, 3 H), 3.77-3.83





(m, 2 H), 3.36 (s, 3 H)









Preparation 15
4-chloro-5,6,7-tris(methyloxy)quinazoline




Step 1. 4,5,6-tris(methyloxy)-1 H-indole-2,3-dione
A solution of 3,4,5-trimethoxyaniline (10 g, 54.6 mmol) in Et2O (100 mL) was treated with a solution of 2M HCl in Et2O (40.9 mL, 82 mmol) and the resulting light gray solid was collected and washed with ether to give the HCl salt of the aniline (11.5 g), which was treated with oxalyl chloride (16.72 mL, 191 mmol) and the mixture was heated at 170° C. for 1.5 hours before being cooled to room temperature and treated with MeOH. The resulting mixture was heated to boiling and filtered while hot to give a red solid, which was washed with MeOH and dried in air to give 4,5,6-tris(methyloxy)-1H-indole-2,3-dione (10.5 g, 81%) as a red-orange solid. MS (m/z) 238.0 (M+H+).
Step 2. 6-amino-2,3,4-tris(methyloxy)benzoic acid
A solution of 4,5,6-tris(methyloxy)-1H-indole-2,3-dione (10 g, 42.2 mmol) in 33% aqueous NaOH (70.5 ml, 582 mmol) at 100° C. was treated with 30% aqueous hydrogen peroxide (12.9 ml, 126 mmol) dropwise over 30 min, at a rate to prevent the foaming mixture from overflowing the flask. After addition was complete, the mixture was stirred for 10 minutes before being cooled and diluted by the addition of ice. The mixture was adjusted to pH 8 by addition of concentrated HCl (70 mL) and then acidified to pH 3 with the addition of glacial acetic acid. The solid that formed was collected by filtration, washed with water, and dried under vacuum to give 6-amino-2,3,4-tris(methyloxy)benzoic acid (2.7 g, 28%) as a tan solid. MS (m/z) 228.0 (M+H+).
Step 3. methyl 6-amino-2,3,4-tris(methyloxy)benzoate
A solution of 6-amino-2,3,4-tris(methyloxy)benzoic acid (2.7 g, 11.88 mmol) in THF (20 mL) and MeOH (20 mL) at 25° C. was treated with a solution of 2M TMS-diazomethane in Et2O (14.85 mL, 29.7 mmol) and stirred for 10 minutes before being concentrated to give methyl 6-amino-2,3,4-tris(methyloxy)benzoate (2.87 g, 99%) as a brown solid. MS (m/z) 242.1 (M+H+).
Step 4. 5,6,7-tris(methyloxy)-4(1H)-quinazolinone
A solution of methyl 6-amino-2,3,4-tris(methyloxy)benzoate (2.87 g, 11.90 mmol) and formamidine acetate (3.72 g, 35.7 mmol) in 2-methoxyethanol (25 mL) was stirred at 125° C. for 2 hrs before being concentrated. The residue was suspended in water and the solid was collected by filtration, washed with water, and dried under vacuum (45° C.) to give 5,6,7-tris(methyloxy)-4(1H)-quinazolinone (1 g, 34%) as a light brown solid. MS (m/z) 237.1 (M+H+).
Step 5. 4-chloro-5,6,7-tris(methyloxy)quinazoline
5,6,7-tris(methyloxy)-4(1H)-quinazolinone (0.8 g, 3.39 mmol) was treated with POCl3 (10 ml, 107 mmol) and one drop of DMF, and stirred at 100° C. for 1 hour before being concentrated. The residue was treated with saturated aqueous NaHCO3 and extracted with CH2Cl2. The organic extracts were dried (sodium sulfate) and concentrated to give 4-chloro-5,6,7-tris(methyloxy)quinazoline (770 mg, 89%) as a tan solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.88 (s, 1 H), 7.37 (s, 1 H), 4.04 (s, 3 H), 3.94 (s, 3 H), 3.91 (s, 3 H). MS (m/z) 255.0 (M+H+).
Preparation 16
N-methyl-3-({6-(methyloxy)-7-[(phenylmethyl)oxy]-4-quinazolinyl}amino)-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide




A solution of 3-amino-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (3.0 g, 10.7 mmol) in i-PrOH (100 mL) was treated with 4-chloro-6-(methyloxy)-7-[(phenylmethyl)oxy]quinazoline (3.2 g, 10.7 mmol, prepared according to J. Med. Chem. (1999) 42 5369) and stirred at 80° C. for 2 hours before being concentrated. The residue was washed with petroleum ether and dried to give N-methyl-3-({6-(methyloxy)-7-[(phenylmethyl)oxy]-4-quinazolinyl}amino)-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (4.2 g, 72%) as a brown solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 11.42 (br. s., 1 H), 8.75 (s, 1 H), 8.20 (s, 1 H), 7.80-7.84 (m, 2 H), 7.49-7.57 (m, 4 H), 7.35-7.49 (m, 4 H), 5.34 (s, 2 H), 4.91 (q, J=8.82 Hz, 2 H), 3.99 (s, 3 H), 2.45 (d, J=5.07 Hz, 3 H). MS (m/z) 549.1 (M+H+).
The following compound was prepared with procedures analogous to that described in Preparation 16 using the specified quinazoline and aniline starting materials:













Name
Structure
Quinazoline
Aniline







4-(dimethylamino)-N- methyl-3-[(6-nitro-4- quinazolinyl)amino]benzene sulfonamide




4-chloro-6- nitroquinazoline
3-amino-4- (dimethylamino)- N-methylbenzene- sulfonamide









Example 1
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide hydrochloride




To a mixture of 3-amino-4-(dimethylamino)-N-methylbenzenesulfonamide (0.100 g, 0.434 mmol) and 4-chloro-6,7-bis(methyloxy)quinazoline (0.075 g, 0.334 mmol) in isopropanol (2 mL) was added HCl (0.334 mL, 0.334 mmol). The resulting mixture was then subjected to microwave irradiation (150° C., 6 bar) for 15 minutes. The mixture was then concentrated and purified via column chromatography (5-10% MeOH/CH2Cl2) to give an orange solid that was triturated with EtOAc and dried to afford 3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide (54 mg, 35% yield) as orange crystals.
The following compounds were prepared with procedures analogous to that described in Example 1 using 4-chloro-6,7-bis(methyloxy)quinazoline and the specified aniline as starting materials:













Ex.
Name
Structure
Aniline







2
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methylbenzenesulfonamide hydrochloride 




3-amino-N- methylbenzene sulfonamide 


 


3
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N-methyl-4-(4- morpholinyl)benzenesulfonamide trifluoroacetate




3-amino-N-methyl-4- (4-morpholinyl) benzene sulfonamide


 


4
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-4- [ethyl(methyl)amino]-N- methylbenzenesulfonamide




3-amino-4- [ethyl(methyl)amino]- N-methylbenzene sulfonamide 


 


5
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N-methyl-4-[(1- methylethyl)oxy]benzenesulfonamide




3-amino-N-methyl-4- [(1-methylethyl)oxy] benzenesulfonamide 


 


6
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N-methyl-4- (methyloxy)benzenesulfonamide




3-amino-N-methyl-4- (methyloxy)benzene sulfonamide 


 


7
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N-methyl-4- (methylthio)benzenesulfonamide trifluoroacetate




3-amino-N-methyl-4- (methylthio)benzene sulfonamide









Example 8
4-(dimethylamino)-N-methyl-3-(4-quinazolinylamino)benzenesulfonamide trifluoroacetate




A mixture of 3-amino-4-(dimethylamino)-N-methylbenzenesulfonamide (136 mg, 0.59 mmol) and 4-chloroquinazoline (75 mg, 0.46 mmol) in i-PrOH (2 mL) was subjected to microwave irradiation (150° C., 6 bar) for 30 minutes before being filtered and subjected to reverse phase HPLC (10-50% MeCN/water/0.1% TFA; Sunfire C18 OBD 5 μM, 30×75 mm) to give an oil, which was dissolved in water and lypophilized to give 4-(dimethylamino)-N-methyl-3-(4-quinazolinylamino)benzenesulfonamide trifluoroacetate (30 mg, 13%) as an orange solid.
The following compounds were prepared with procedures analogous to that described in Example 8 using the specified quinazoline and aniline starting materials:














Ex.
Name
Structure
Quinazoline
Aniline



















9
3-[(6-chloro-4- quinazolinyl)amino]-4- (dimethylamino)-N- methylbenzenesulfonamide 




4,6- dichloro- quinazoline 
3-amino-4- (dimethylamino)- N- methylbenzene- sulfonamide 


 


10
4-(dimethylamino)-N- methyl-3-{[6-(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro-6- (methyloxy) quinazoline
3-amino-4- (dimethylamino)- N- methylbenzene- sulfonamide









Example 11
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide hydrochloride




A mixture of 4-chloro-6,7-bis(methyloxy)quinazoline (100 mg, 0.445 mmol) and 3-amino-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (139 mg, 0.490 mmol) in i-PrOH (2 mL) was subjected to microwave irradiation (100° C., 2 bar) for 30 minutes before being cooled to room temperature. The solid that formed was collected by filtration and washed with isopropanol to give 3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide hydrochloride (160 mg, 70%) as a pale yellow solid.
The following compounds were prepared with procedures analogous to that described in Example 11 using the specified quinazoline and aniline starting materials:














Ex.
Name
Structure
Quinazoline
Aniline







12
3-[(6-chloro-4- quinazolinyl)amino]-N- methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4,6- dichloro- quinazoline 
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


13
3-(7,8- dihydro[1,4]dioxino[2,3- g]quinazolin-4-ylamino)-4- (dimethylamino)-N- methylbenzenesulfonamide bis(trifluoroacetate)




4-chloro-7,8- dihydro[1,4] dioxino[2,3- g]quinazoline 
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide


 


14
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-4-(4,4- difluoro-1-piperidinyl)-N- methylbenzenesulfonamide trifluoroacetate 




4-chloro-6,7- bis(methyloxy) quinazoline 
3-amino-4-(4,4- difluoro-1- piperidinyl)-N- methylbenzene sulfonamide 


 


15
3-[(6-chloro-4- quinazolinyl)amino]-4-(4,4- difluoro-1-piperidinyl)-N- methylbenzenesulfonamide trifluoroacetate




4,6- dichloro- quinazoline
3-amino-4-(4,4- difluoro-1- piperidinyl)-N- methylbenzene sulfonamide


 


16
3-({7-[(3-chloropropyl)oxy]- 4-quinazolinyl}amino)-4- (dimethylamino)-N- methylbenzenesulfonamide




4-chloro-7- [(3- chloropropyl) oxy] quinazoline
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide


 


17
3-[(6,7-bis{[2- (methyloxy)ethyl]oxy}-4- quinazolinyl)amino]-4- (dimethylamino)-N- methylbenzenesulfonamide trifluoroacetate




4-chloro-6,7- bis{[2- (methyloxy) ethyl]oxy} quinazoline
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide


 


18
3-[(6,7-bis{[2- (methyloxy)ethyl]oxy}-4- quinazolinyl)amino]-N- methyl-4- (methyloxy) benzenesulfonamide  hydrochloride




4-chloro-6,7- bis{[2- (methyloxy) ethyl]oxy} quinazoline
3-amino-N- methyl-4- (methyloxy) benzene- sulfonamide 


 


19
3-[(6,7-bis{[2- (methyloxy)ethyl]oxy}-4- quinazolinyl)amino]-N- methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4-chloro-6,7- bis{[2- (methyloxy) ethyl]oxy} quinazoline 
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


20
3-[(6,7-bis{[2- (methyloxy)ethyl]oxy}-4- quinazolinyl)amino]-N- methyl-4- (methylthio) benzenesulfonamide




4-chloro-6,7- bis{[2- (methyloxy) ethyl]oxy} quinazoline 
3-amino-N- methyl-4- (methylthio) benzene- sulfonamide 


 


21
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2R)-2- (trifluoromethyl)-1- pyrrolidinyl] benzenesulfonamide trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4-[(2R)- 2- (trifluoromethyl)- 1- pyrrolidinyl] benzene- sulfonamide


 


22
3-[(6-chloro-4- quinazolinyl)amino]-N- methyl-4-[(2R)-2- (trifluoromethyl)-1- pyrrolidinyl] benzenesulfonamide trifluoroacetate




4,6- dichloro- quinazoline 
3-amino-N- methyl-4-[(2R)- 2- (trifluoromethyl)- 1- pyrrolidinyl] benzene- sulfonamide 


 


23
5-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-2- fluoro-N- methylbenzenesulfonamide hydrochloride




4-chloro-6,7- bis(methyloxy) quinazoline
5-amino-2- fluoro-N- methylbenzene sulfonamide


 


24
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4- [(trifluoromethyl)oxy] benzenesulfonamide trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline 
3-amino-N- methyl-4- [(trifluoromethyl) oxy]benzene- sulfonamide


 


25
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-(1- piperidinyl) benzenesulfonamide trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4-(1- piperidinyl) benzene- sulfonamide


 


26
3-[(6-chloro-4- quinazolinyl)amino]-N- methyl-4-(1- piperidinyl) benzenesulfonamide trifluoroacetate 




4,6- dichloro- quinazoline
3-amino-N- methyl-4-(1- piperidinyl) benzene- sulfonamide


 


27
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-4-(2,5- dimethyl-1-pyrrolidinyl)-N- methylbenzenesulfonamide trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-4-(2,5- dimethyl-1- pyrrolidinyl)-N- methylbenzene sulfonamide 


 


28
3-[(6-chloro-4- quinazolinyl)amino]-4-(2,5- dimethyl-1-pyrrolidinyl)-N- methylbenzenesulfonamide trifluoroacetate 




4,6- dichloro- quinazoline
3-amino-4-(2,5- dimethyl-1- pyrrolidinyl)-N- methylbenzene sulfonamide


 


29
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2- trifluoroethyl)thio] benzenesulfonamide  hydrochloride




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) thio]benzene- sulfonamide


 


30
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methylbenzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methylbenzene sulfonamide









Example 31
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide hydrochloride




A mixture of 4-chloro-5,7-bis(methyloxy)quinazoline (75 mg, 0.334 mmol, prepared according to J. Med. Chem. (2006) 49 6465) and 3-amino-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (95 mg, 0.334 mmol) in i-PrOH (2 mL) was subjected to microwave irradiation (100° C., 1 bar) for 30 minutes before being cooled to room temperature. The solid was collected by filtration, washed with i-PrOH, and dried under vacuum to give 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide hydrochloride (130 mg, 76%) as a yellow soid.
The following compounds were prepared with procedures analogous to that described in Example 31 using the specified quinazoline and aniline starting materials:














Ex.
Name
Structure
Quinazoline
Aniline







32
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-4- (dimethylamino)-N- methylbenzenesulfonamide




4-chloro-5,7- bis(methyloxy) quinazoline 
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide


 


33
3-(5-chloro-6,7- dimethoxyquinazolin-4- ylamino)-N- methylbenzenesulfonamide trifluoroacetate




4,5-dichloro- 6,7- bis(methyloxy) quinazoline
3-amino-N- methylbenzene sulfonamide


 


34
4-methoxy-3-(7-methoxy-6- (3- morpholinopropoxy) quinazolin-4-ylamino)-N- methylbenzenesulfonamide bis(trifluoroacetate)




4-chloro-7- (methyloxy)- 6-{[3-(4- morpholinyl) propyl]oxy} quinazoline (J. Med. Chem. (2004) 47 871)
3-amino-N- methyl-4- (methyloxy) benzene- sulfonamide


 


35
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[methyl(2,2,2- trifluoroethyl)amino] benzenesulfonamide trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- [methyl(2,2,2- trifluoroethyl) amino]benzene- sulfonamide 


 


36
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[methyl(2,2,2- trifluoroethyl)amino] benzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- [methyl(2,2,2- trifluoroethyl) amino]benzene- sulfonamide 


 


37
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4- (methyloxy) benzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- (methyloxy) benzene- sulfonamide


 


38
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-4- fluoro-N- methylbenzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline 
3-amino-4- fluoro-N- methylbenzene sulfonamide


 


39
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-4- chloro-N- methylbenzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-4- chloro-N- methylbenzene sulfonamide


 


40
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-(4- morpholinyl) benzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4-(4- morpholinyl) benzene- sulfonamide 


 


41
5-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-2- fluoro-N-methyl-4- (methyloxy) benzenesulfonamide  hydrochloride




4-chloro-6,7- bis(methyloxy) quinazoline
5-amino-2- fluoro-N- methyl-4- (methyloxy) benzene- sulfonamide 


 


42
3-{[6-(ethyloxy)-7- (methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2- trifluoroethyl)oxy]  benzenesulfonamide  hydrochloride




4-chloro-6- (ethyloxy)-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide 


 


43
4-(dimethylamino)-3-(7- methoxy-6-(3- morpholinopropoxy) quinazolin-4-ylamino)-N- methylbenzenesulfonamide bis(formate)




4-chloro-7- (methyloxy)- 6-{[3-(4- morpholinyl) propyl]oxy} quinazoline
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide


 


44
N-methyl-3-{[6-[(1- methylethyl)oxy]-7- (methyloxy)-4- quinazolinyl]amino}-4- [(2,2,2- trifluoroethyl)oxy]  benzenesulfonamide  hydrochloride




4-chloro-6- [(1- methylethyl) oxy]-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


45
3-({7-[(3-chloropropyl)oxy]- 4-quinazolinyl}amino)-N- methyl-4-[(2,2,2- trifluoroethyl)oxy]  benzenesulfonamide




4-chloro-7- [(3- chloropropyl) oxy] quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


46
3-{[5-(ethyloxy)-7- (methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2- trifluoroethyl)oxy]  benzenesulfonamide




4-chloro-5- (ethyloxy)-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


47
N-methyl-3-{[5-[(1- methylethyl)oxy]-7- (methyloxy)-4- quinazolinyl]amino}-4- [(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4-chloro-5- [(1- methylethyl) oxy]-7- (methyloxy) quinazoline 
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide 


 


48
N-methyl-4-[(2,2,2- trifluoroethyl)oxy]-3-{[5,6,7- tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide hydrochloride




4-chloro- 5,6,7- tris(methyloxy) quinazoline 
3-amino-N- methyl-4- ((2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


49
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2-trifluoro-1- methylethyl)oxy] benzenesulfonamide  trifluoroacetate




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2-trifluoro- 1- methylethyl)oxy] benzene- sulfonamide


 


50
5-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-2- fluoro-N-methyl-4-[(2,2,2- trifluoroethyl)oxy]  benzenesulfonamide  trifluoroacetate




4-chloro-5,7- bis(methyloxy) quinazoline
5-amino-2- fluoro-N- methyl-4- [(2,2,2- trifluoroethyl) oxy] benzene- sulfonamide


 


51
5-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-2- fluoro-N-methyl-4- (methyloxy) benzenesulfonamide  trifluoroacetate




4-chloro-5,7- bis(methyloxy) quinazoline
5-amino-2- fluoro-N- methyl-4- (methyloxy) benzene- sulfonamide


 


52
5-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-2- fluoro-N-methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide  hydrochloride




4-chloro-6,7- bis(methyloxy) quinazoline
5-amino-2- fluoro-N- methyl-4- [(2,2,2- trifluoroethyl) oxy] benzene- sulfonamide


 


53
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4- (methylthio) benzenesulfonamide  hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- (methylthio) benzene- sulfonamide


 


54
3-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4- [(trifluoromethyl)oxy] benzenesulfonamide  hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- [(trifluoromethyl) oxy]benzene- sulfonamide


 


55
N-methyl-4- [(trifluoromethyl)oxy]-3- {[5,6,7-tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro- 5,6,7- tris(methyloxy) quinazoline
3-amino-N- methyl-4- [(trifluoromethyl) oxy]benzene- sulfonamide


 


56
5-{[5,7-bis(methyloxy)-4- quinazolinyl]amino}-4- (dimethylamino)-2-fluoro-N- methylbenzenesulfonamide hydrochloride




4-chloro-5,7- bis(methyloxy) quinazoline
5-amino-4- (dimethylamino)- 2-fluoro-N- methylbenzene sulfonamide


 


57
5-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-4- (dimethylamino)-2-fluoro-N- methylbenzenesulfonamide hydrochloride




4-chloro-6,7- bis(methyloxy) quinazoline
5-amino-4- (dimethylamino)- 2-fluoro-N- methylbenzene sulfonamide


 


58
2-fluoro-N-methyl-4-[(2,2,2- trifluoroethyl)oxy]-5-{[5,6,7- tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro 5,6,7- tris(methyloxy) quinazoline
5-amino-2- fluoro-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


59
4-chloro-N-methyl-3-{[5,6,7- tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro- 5,6,7- tris(methyloxy) quinazoline
3-amino-4- chloro-N- methylbenzene sulfonamide


 


60
N-methyl-3-[(7-(methyIoxy)- 5-{[2-(methyloxy)ethyl]oxy}- 4-quinazolinyl)amino]-4- [(2,2,2- trifluoroethyl)oxy]benzene- sulfonamide hydrochloride




4-chloro-7- (methyloxy)- 5-{[2- (methyloxy) ethyl]oxy} quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


61
N-methyl-3-{[7-(methyloxy)- 5-(tetrahydro-2H-pyran-4- yloxy)-4- quinazolinyl]amino}-4- [(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4-chloro-7- (methyloxy)- 5- (tetrahydro- 2H-pyran-4- yloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


62
N-methyl-4-[(2,2,2-trifluoro- 1-methylethyl)oxy]-3- {[5,6,7-tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro- 5,6,7- tris(methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2-trifluoro- 1- methylethyl)oxy] benzene- sulfonamide


 


63
N-methyl-4- (methylsulfonyl)-3-{[5,6,7- tris(methyloxy)-4- quinazolinyl]amino}benzene sulfonamide trifluoroacetate




4-chloro- 5,6,7- tris(methyloxy) quinazoline
3-amino-N- methyl-4- (methylsulfonyl) benzene- sulfonamide


 


64
3-{[5-(ethyloxy)-7- (methyloxy)-4- quinazolinyl]amino}-N- methyl-4- [(trifluoromethyl)oxy] benzenesulfonamide




4-chloro-5- (ethyloxy)-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(trifluoromethyl) oxy]benzene- sulfonamide


 


65
N-methyl-3-{[5-[(1- methylethyl)oxy]-7- (methyloxy)-4- quinazolinyl]amino}-4- [(trifluoromethyl)oxy] benzenesulfonamide




4-chloro-5- [(1- methylethyl) oxy]-7- (methyloxy) quinazoline 
3-amino-N- methyl-4- [(trifluoromethyl) oxy]benzene- sulfonamide


 


66
3-{[5-(ethyloxy)-7- (methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2-trifluoro-1- methylethyl)oxy] benzenesulfonamide




4-chloro-5- (ethyloxy)-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2-trifluoro- 1- methylethyl)oxy] benzene- sulfonamide


 


67
N-methyl-3-{[5-[(1- methylethyl)oxy]-7- (methyloxy)-4- quinazolinyl]amino}-4- [(2,2,2-trifluoro-1- methylethyl)oxy] benzenesulfonamide




4-chloro-5- [(1- methylethyl) oxy]-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2-trifluoro- 1- methylethyl)oxy] benzene- sulfonamide 


 


68
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4- (methylsulfonyl) benzenesulfonamide  trifluoroacetate




4-chloro-6,7- bis(methyloxy) quinazoline
3-amino-N- methyl-4- (methylsulfonyl) benzene- sulfonamide


 


69
3-{[6,7-bis(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2-trifluoro-1- methylethyl)oxy] benzenesulfonamide




4-chloro-6,7- bis(methyloxy) quinazoline 
3-amino-N- methyl-4- [(2,2,2-trifluoro- 1- methylethyl)oxy] benzene- sulfonamide


 


70
3-{[6-iodo-7-(methyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4-chloro-6- iodo-7- (methyloxy) quinazoline
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide


 


71
3-{[6,7-bis(ethyloxy)-4- quinazolinyl]amino}-N- methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide




4-chloro-6,7- bis(ethyloxy) quinazoline (J. Med. Chem. (2005) 48 7445)
3-amino-N- methyl-4- [(2,2,2- trifluoroethyl) oxy]benzene- sulfonamide 


 


72
4-(dimethylamino)-N- methyI-3-((7- nitroquinazolin-4- yl)amino) benzenesulfonamide  hydrochloride




4-chloro-7- nitro- quinazoline
3-amino-4- (dimethylamino)- N- methylbenzene sulfonamide









Example 73
4-(dimethylamino)-N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide hydrochloride




A mixture of 3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-4-(dimethylamino)-N-methylbenzenesulfonamide (50 mg, 0.111 mmol) and morpholine (0.048 mL, 0.556 mmol) in MeOH (1 mL) was stirred at 90° C. for 3 days before being cooled and concentrated. The residue was triturated with Et2O-EtOAc and the resulting solid was collected by filtration, washed with Et2O, and dried to give 4-(dimethylamino)-N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide hydrochloride (33 mg, 48%) as an off-white solid.
The following compound was prepared with procedures analogous to that described in Example 73 using the specified quinazoline starting material:













Ex.
Name
Structure
Quinazoline SM







74
N-methyl-3-[(7-{[3-(4- morpholinyl)propyl]oxy}-4- quinazolinyl)amino]-4- [(2,2,2- trifluoroethyl)oxy] benzenesulfonamide  trifluoroacetate




3-({7-[(3- chloropropyl)oxy]- 4- quinazolinyl}amino)- N-methyl-4-[(2,2,2- trifluoroethyl)oxy] benzenesulfonamide









Example 75
N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide




A mixture of 3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (150 mg, 0.297 mmol) in MeOH (3 mL) was treated with a solution of 25% sodium methoxide in methanol (3 g, 13.88 mmol) and subjected to microwave irradiation (100° C.) for 10 minutes before being cooled and neutralized with the addition of concentrated HCl. The resulting solution was diluted with saturated aqueous NaHCO3 and extracted with CH2Cl2. The organic extracts were dried (sodium sulfate), concentrated, and subjected to reverse phase HPLC (30-70% MeCN/aq. NH4OH pH 10, XBridge C18 OBD 30×150 mm, 5 μm) to give N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (37 mg, 25%) as a white solid.
The following compound was prepared with procedures analogous to that described in Example 75 using the specified quinazoline starting material:













Ex.
Name
Structure
Quinazoline SM







76
4-(dimethylamino)-N- methyl-3-[(7-{[3- (methyloxy)propyl]oxy}-4- quinazolinyl)amino] benzenesulfonamide




3-({7-[(3- chloropropyl)oxy]- 4- quinazolinyl}amino)- 4-(dimethylamino)- N-methyl- benzenesulfonamide









Example 77
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide trifluoroacetate




A mixture of 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylthio)benzenesulfonamide (200 mg, 0.476 mmol) and sodium perborate tetrahydrate (220 mg, 1.427 mmol) in AcOH (1.902 ml) was heated at 50° C. for 2 hours before being filtered and extracted with EtOAc. The organic extract was washed with water, saturated aqueous NaHCO3, and brine, then dried (sodium sulfate), concentrated, and subjected to reverse phase HPLC (Sunfire prep C18 OBD, 30×150 mm, 10-50% Water/CH3CN+0.1% TFA) to give 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide trifluoroacetate (28 mg, 10%) as a white solid.
Example 78
3-(7-hydroxy-6-methoxyquinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoroethoxy)benzenesulfonamide




A mixture of N-methyl-3-({6-(methyloxy)-7-[(phenylmethyl)oxy]-4-quinazolinyl}amino)-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (4.2 g, 7.66 mmol) in EtOAc (120 mL), MeOH (40 mL), and DMF (40 mL) was treated with 60% AcOH (0.4 mL) and Pd/C (400 mg) was stirred under H2 (balloon) for 2 hours before being filtered and concentrated to give 3-(7-hydroxy-6-methoxyguinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoroethoxy)benzenesulfonamide (3.2 g, 91%) as a yellow solid.
Example 79
3-(7-isopropoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide




A solution of 3-{[7-hydroxy-6-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide (170 mg, 0.37 mmol) in DMF (5 mL) was treated with 2-bromopropane (181 mg, 1.48 mmol) and K2CO3 (154 mg, 1.11 mmol) and stirred at 60° C. for 16 hours before being poured into water. The precipitate that formed was collected by filtration, washed with MeOH, and dried to give 3-(7-isopropoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide (80 mg, 43%) as an off-white solid.
The following compounds were prepared with procedures analogous to that described in Example 79 using the indicated alkyl halide:













Ex.
Name
Structure
Alkyl Halide







80
3-(7-ethoxy-6-methoxy- quinazolin-4-ylamino)-N- methyl-4-(2,2,2-trifluoro- ethoxy)- benzenesulfonamide trifluoroacetate




bromoethane


 


81
3-[6-methoxy-7-(2-methoxy- ethoxy)-quinazolin-4- ylamino]-N-methyl-4-(2,2,2- trifluoro-ethoxy)- benzenesulfonamide trifluoroacetate




1-bromo-2- (methyloxy)ethane









Examples 82 & 83
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide hydrochloride (enantiomer 1) & 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide hydrochloride (enantiomer 2)




A racemic mixture of 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide hydrochloride (528 mg) was subjected to chiral SFC (Chiralpak OJ-H, 15% MeOH cosolvent) to provide 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide hydrochloride (unassigned enantiomer 1, 33mg) & 3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide hydrochloride (unassigned enantiomer 2, 49 mg).
Example 84
3-((6-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide




A mixture of 4-(dimethylamino)-N-methyl-3-((6-nitroquinazolin-4-yl)amino)benzene-sulfonamide (715 mg, 1.78 mmol) in MeOH (50 mL) was treated with 10% Pd/C (189 mg, 0.178 mmol) and stirred under H2 (balloon) at 25° C. for 2.5 hours before being filtered through celite and concentrated. The residue was dissolved in CH2Cl2/MeOH, washed with saturated aqueous NaHCO3, dried (Na2SO4), and concentrated to give 3-((6-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide (433 mg, 59%) as a yellow solid.
The following compound was prepared with procedures analogous to that described in Example 84:













Ex.
Name
Structure
Quinazoline SM







85
3-((7-aminoquinazolin-4- yl)amino)-4- (dimethylamino)-N- methylbenzenesulfonamide




4-(dimethylamino)-N- methyl-3-[(7-nitro-4- quinazolinyl)amino] benzenesulfonamide









Example 86
4-(dimethylamino)-3-((6-(dimethylamino)quinazolin-4-yl)amino)-N-methylbenzenesulfonamide bis(trifluoroacetate) &
Example 87
4-(dimethylamino)-N-methyl-3-((6-(methylamino)quinazolin-4-yl)amino)benzenesulfonamide bis(trifluoroacetate)




A mixture of 3-((6-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzene-sulfonamide (129 mg, 0.346 mmol) in CH2Cl2 (3 mL) was treated with AcOH (0.022 mL, 0.381 mmol) and 37% aqueous formaldehyde (0.028 mL, 0.381 mmol) and stirred at 25° C. After 5 minutes, sodium triacetoxyborohydride (110 mg, 0.520 mmol) was added and the mixture was stirred for 2 hours before being quenched with the addition of saturated aqueous NaHCO3 and extracted with a CH2Cl2/MeOH mixture. The organic extract was dried (Na2SO4), concentrated, and subjected to reverse phase HPLC (10-40% MeCN/H2O/0.1% TFA on a SunfireTM Prep C18 OBDTM 5 μm 30×150 mm column) to give 4-(dimethylamino)-N-methyl-3-((6-(methylamino)quinazolin-4-yl)amino)benzene-sulfonamide (3 mg, 1.4%) as a yellow solid and 4-(dimethylamino)-3-((6-(dimethylamino)quinazolin-4-yl)amino)-N-methylbenzenesulfonamide (3.6 mg, 1.6%) as a yellow solid.
The following compound was prepared with procedures analogous to that described in Examples 86 & 87:













Ex.
Name
Structure
Quinazoline SM







88
4-(dimethylamino)-N- methyl-3-((7- (methylamino)quinazolin-4- yl)amino) benzenesulfonamide




3-((7- aminoquinazolin-4- yl)amino)-4- (dimethylamino)-N- methylbenzene- sulfonamide









Spectroscopic Data For Examples 1-88














Ex.
Name
tR (min)
MS (m/z)
1H NMR



















1
3-{[6,7-bis(methyloxy)-4-
2.06a
418
1H NMR (400 MHz, DMSO-d6) δ 11.38 (br.



quinazolinyl]amino}-4-


s., 1H), 8.81 (s, 1H), 8.29 (s, 1H), 7.59-



(dimethylamino)-N-


7.71 (m, 2H), 7.31-7.39 (m, 2H), 7.23 (d,



methylbenzenesulfonamide


J = 9.54 Hz, 1H), 4.00 (s, 3H), 4.01 (s,



hydrochloride


3H), 2.82 (s, 6H), 2.43 (d, J = 4.27 Hz,






3H)


2
3-{[6,7-bis(methyloxy)-4-
1.81a
375.1
1H NMR (400 MHz, DMSO-d6) δ 10.98 (br.



quinazolinyl]amino}-N-


s., 1H), 8.85 (s, 1H), 8.05-8.18 (m, 3H),



methylbenzenesulfonamide


7.73 (t, J = 7.91 Hz, 1H), 7.67 (d, J = 7.78



hydrochloride


Hz, 1H), 7.59 (q, J = 4.43 Hz, 1H), 7.29 (s,






1H), 4.02 (d, J = 3.76 Hz, 6H), 2.48 (s, 3H)


3
3-{[6,7-bis(methyloxy)-4-
1.78a
459.9
1H NMR (400 MHz, DMSO-d6) δ 10.66 (br.



quinazolinyl]amino}-N-


s., 1H), 8.76 (s, 1H), 8.05 (s, 1H), 7.92-



methyl-4-(4-morpholinyl)benzene-


7.98 (m, 1H), 7.71 (dd, J = 2.01, 8.53 Hz,



sulfonamide trifluoroacetate


1H), 7.44 (q, J = 5.02 Hz, 1H), 7.37 (d, J =






8.53 Hz, 1H), 7.28 (s, 1H), 3.99 (s, 3H),






4.01 (s, 3H), 3.50-3.60 (m, 4H), 2.96-






3.03 (m, 4H), 2.45 (d, J = 4.77 Hz, 3H)


4
3-{[6,7-bis(methyloxy)-4-
1.91a
432.2
1H NMR (400 MHz, DMSO-d6) δ 11.29 (br.



quinazolinyl]amino}-4-


s., 1H), 8.80 (s, 1H), 8.29 (s, 1H), 7.62-



[ethyl(methyl)amino]-N-


7.71 (m, 2H), 7.32-7.39 (m, 2H), 7.26 (d,



methylbenzenesulfonamide


J = 8.53 Hz, 1H), 3.99 (s, 3H), 4.01 (s,






3H), 3.07 (q, J = 6.94 Hz, 2H), 2.79 (s,






3H), 2.43 (d, J = 4.77 Hz, 3H), 0.96 (t, J =






7.03 Hz, 3H)


5
3-{[6,7-bis(methyloxy)-4-
1.93a
433.1
1H NMR (400 MHz, DMSO-d6) δ 11.09 (br.



quinazolinyl]amino}-N-methyl-


s., 1H), 8.76 (s, 1H), 8.12 (s, 1H), 7.78-



4-(methyloxy)benzenesulfon-


7.85 (m, 2H), 7.40-7.49 (m, 2H), 7.30 (s,



amide


1H), 4.00 (d, J = 7.55 Hz, 6H), 3.89 (s,






3H), 2.45 (d, J = 4.78 Hz, 3H)


6
3-{[6,7-bis(methyloxy)-4-
1.75a
405
1H NMR (400 MHz, DMSO-d6) δ 10.93 (br.



quinazolinyl]amino}-N-methyl-


s., 1H), 8.77 (s, 1H), 8.05 (s, 1H), 7.80-



4-[(1-methylethyl)oxy]benzene-


7.85 (m, 1H), 7.76 (dd, J = 2.27, 8.81 Hz,



sulfonamide


1H), 7.39-7.49 (m, 2H), 7.29 (s, 1H),






4.78 (quin, J = 5.98 Hz, 1H), 4.01 (s, 3H),






3.99 (s, 3H), 2.45 (d, J = 5.04 Hz, 3H),






1.21 (s, 3H), 1.19 (s, 3H)


7
3-{[6,7-bis(methyloxy)-4-
1.80a
421.1
1H NMR (400 MHz, methanol-d4) δ 7.08



quinazolinyl]amino}-N-


(s, 1H), 6.41 (s, 1H), 6.32-6.37 (m, 1H),



methyl-4-(methylthio)ben-


6.30 (d, J = 1.76 Hz, 1H), 6.10 (d, J = 8.53



zenesulfonamide trifluoroacetate


Hz, 1H), 5.73 (s, 1H), 2.54 (s, 3H), 2.56 (s,






3H), 1.05 (s, 3H), 1.02 (s, 3H)


8
4-(dimethylamino)-N-methyl-
1.67a
358.1
1H NMR (400 MHz, DMSO-d6) δ ppm



3-(4-quinazolinylamino)benzene-


10.77 (br. s., 1 H), 8.78-8.87 (m, 1 H),



sulfonamide trifluoroacetate


8.75 (s, 1 H), 8.06 (dd, J = 8.91, 1.88 Hz, 1






H), 7.87 (d, J = 9.04 Hz, 1 H), 7.74 (d,






J = 2.01 Hz, 1 H), 7.63 (dd, J = 8.78, 2.26






Hz, 1 H), 7.32 (q, J = 5.02 Hz, 1 H), 7.23 (d,






J = 8.78 Hz, 1 H), 2.80 (s, 6 H), 2.43 (d,






J = 5.02 Hz, 3 H)


9
3-[(6-chloro-4-
1.89a
392.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl)amino]-4-


11.32 (br. s., 1 H), 8.85 (s, 1 H), 8.19 (d,



(dimethylamino)-N-


J = 2.51 Hz, 1 H), 7.87 (d, J = 9.29 Hz, 1 H),



methylbenzenesulfonamide


7.77 (dd, J = 9.29, 2.51 Hz, 1 H), 7.72 (d,






J = 2.26 Hz, 1 H), 7.67 (dd, J = 8.53, 2.26






Hz, 1 H), 7.33 (q, J = 4.94 Hz, 1 H), 7.25 (d,






J = 8.78 Hz, 1 H), 3.97 (s, 3 H), 2.82 (s, 6






H), 2.39-2.47 (m, 3 H)


10
4-(dimethylamino)-N-methyl-
1.89a
388.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.41



3-{[6-(methyloxy)-4-


(s, 1 H), 8.36 (s, 1 H), 7.97 (d, J = 2.26 Hz,



quinazolinyl]amino}benzene-


1 H), 7.73 (s, 1 H), 7.68 (dd, J = 8.78, 2.26



sulfonamide trifluoroacetate


Hz, 1 H), 7.40-7.50 (m, 2 H), 7.20 (s, 1






H), 4.88 (q, J = 8.78 Hz, 2 H), 3.95 (s, 3 H),






3.94 (s, 3 H), 2.46 (d, J = 4.77 Hz, 3 H)


11
3-{[6,7-bis(methyloxy)-4-
2.10a
473.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.91



quinazolinyl]amino}-N-


(br. s., 1 H), 8.57 (br. s., 1 H), 8.49 (s, 1



methyl-4-[(2,2,2-trifluoro-


H), 7.77-7.95 (m, 3 H), 7.67-7.77 (m, 1



ethyl)oxy]benzenesulfonamide


H), 7.47 (d, J = 8.28 Hz, 2 H), 4.88 (q,



hydrochloride


J = 8.70 Hz, 2 H), 2.46 (d, J = 4.77 Hz, 3 H)


12
3-[(6-chloro-4-
2.14a
447.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl)amino]-N-


11.05 (br. s., 1 H), 8.78 (s, 1 H), 8.24 (s, 1



methyl-4-[(2,2,2-


H), 7.69 (d, J = 2.26 Hz, 1 H), 7.63-7.68



trifluoroethyl)oxy]benzene-


(m, 1 H), 7.29-7.35 (m, 2 H), 7.23 (d,



sulfonamide


J = 8.78 Hz, 1 H), 4.41-4.59 (m, 4 H), 2.80






(s, 6 H), 2.43 (d, J = 5.02 Hz, 3 H)


13
3-(7,8-
1.87a
416.1
1H NMR (400 MHz, DMSO-d6) δ ppm



dihydro[1,4]dioxino[2,3-


10.63 (br. s., 1 H), 8.77 (s, 1 H), 8.07 (s, 1



g]quinazolin-4-ylamino)-4-


H), 7.97 (d, J = 2.01 Hz, 1 H), 7.69 (dd,



(dimethylamino)-N-


J = 8.53, 2.01 Hz, 1 H), 7.38-7.51 (m, 2



methylbenzenesulfonamide


H), 7.30 (s, 1 H), 4.02 (s, 3 H), 3.98 (s, 3



bis(trifluoroacetate)


H), 3.12 (t, J = 5.14 Hz, 4 H), 2.45 (d,






J = 5.02 Hz, 3 H), 1.87-2.05 (m, 4 H)


14
3-{[6,7-bis(methyloxy)-4-
2.14a
494.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-4-(4,4-


10.57 (br. s., 1 H), 8.67-8.86 (m, 2 H),



difluoro-1-piperidinyl)-N-


8.06 (dd, J = 8.91, 2.13 Hz, 1 H), 7.93-



methylbenzenesulfonamide


8.01 (m, 1 H), 7.89 (d, J = 9.04 Hz, 1 H),



trifluoroacetate


7.67 (dd, J = 8.53, 2.26 Hz, 1 H), 7.36-






7.49 (m, 2 H), 3.12 (t, J = 5.27 Hz, 4 H),






2.46 (d, J = 4.77 Hz, 3 H), 1.89-2.03 (m, 4 H)


15
3-[(6-chloro-4-
2.24a
468.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.45



quinazolinyl)amino]-4-(4,4-


(br. s., 1 H), 8.44 (s, 1 H), 8.38 (d, J = 9.03



difluoro-1-piperidinyl)-N-


Hz, 1 H), 7.88-7.95 (m, 1 H), 7.54 (dd,



methylbenzenesulfonamide


J = 8.53, 2.01 Hz, 1 H), 7.13-7.36 (m, 4



trifluoroacetate


H), 4.28 (t, J = 6.02 Hz, 2 H), 3.85 (t,






J = 6.53 Hz, 2 H), 2.77 (s, 6 H), 2.43 (s, 3






H), 2.26 (quin, J = 6.21 Hz, 2 H)


16
3-({7-[(3-chloropropyl)oxy]-
2.15a
450.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.95



4-quinazolinyl}amino)-4-


(br. s., 1 H), 8.76 (br. s., 1 H), 8.60 (d,



(dimethylamino)-N-


J = 9.29 Hz, 1 H), 7.76 (d, J = 2.01 Hz, 1 H),



methylbenzenesulfonamide


7.64 (dd, J = 8.66, 2.13 Hz, 1 H), 7.44 (d,






J = 8.78 Hz, 1 H), 7.33 (q, J = 5.19 Hz, 1 H),






7.20-7.29 (m, 2 H), 4.30 (t, J = 5.77 Hz, 2






H), 4.01 (br. s., 2 H), 3.68 (t, J = 12.55 Hz,






2 H), 3.57 (s, 3 H), 3.52 (br. s., 2 H), 3.34






(br. s., 2 H), 3.14 (br. s., 2 H), 2.80 (s, 6






H), 2.40-2.46 (m, 3 H), 2.23 (d, J = 9.03






Hz, 2 H)


17
3-[(6,7-bis{[2-
1.96a
506.2
1H NMR (400 MHz, DMSO-d6) δ ppm



(methyloxy)ethyl]oxy}-4-


11.12 (br. s., 1 H), 8.75 (s, 1 H), 8.19 (s, 1



quinazolinyl)amino]-4-


H), 7.78-7.84 (m, 2 H), 7.41-7.49 (m, 2



(dimethylamino)-N-


H), 7.36 (s, 1 H), 4.35 (q, J = 4.85 Hz, 4 H),



methylbenzenesulfonamide


3.89 (s, 3 H), 3.76-3.83 (m, 4 H), 3.37 (s,



trifluoroacetate


3 H), 3.37 (s, 3 H), 2.46 (d, J = 5.02 Hz, 3 H)


18
3-[(6,7-bis{[2-
1.90a
493.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.39



(methyloxy)ethyl]oxy}-4-


(br. s., 1 H), 8.35 (s, 1 H), 7.96 (d, J = 2.01



quinazolinyl)amino]-N-


Hz, 1 H), 7.77 (s, 1 H), 7.68 (dd, J = 8.53,



methyl-4-(methyloxy)benzene-


2.26 Hz, 1 H), 7.39-7.51 (m, 2 H), 7.23



sulfonamide hydrochloride


(s, 1 H), 4.88 (q, J = 8.70 Hz, 2 H), 4.22-






4.32 (m, 4 H), 3.70-3.82 (m, 4 H), 3.37






(s, 3 H), 3.36 (s, 3 H), 2.46 (s, 3 H)


19
3-[(6,7-bis{[2-
2.08a
561.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.58



(methyloxy)ethyl]oxy}-4-


(br. s., 1 H), 8.29 (s, 1 H), 7.85 (s, 1 H),



quinazolinyl)amino]-N-


7.62-7.74 (m, 2 H), 7.56 (d, J = 8.28 Hz, 1



methyl-4-[(2,2,2-trifluoro-


H), 7.42-7.51 (m, 1 H), 7.22 (s, 1 H),



ethyl)oxy]benzenesulfonamide


4.22-4.33 (m, 4 H), 3.71-3.81 (m, 4 H),






3.38 (s, 3 H), 3.36 (s, 3 H), 2.46 (s, 6 H)


20
3-[(6,7-bis{[2-
1.97a
509.1
1H NMR (400 MHz, DMSO-d6) δ ppm



(methyloxy)ethyl]oxy}-4-


11.06 (br. s., 1 H), 8.74 (s, 1 H), 8.06 (s, 1



quinazolinyl)amino]-N-


H), 7.69 (d, J = 2.01 Hz, 1 H), 7.64 (dd,



methyl-4-(methylthio)benzene-


J = 8.78, 2.26 Hz, 1 H), 7.54 (d, J = 9.03 Hz,



sulfonamide


1 H), 7.36 (q, J = 4.94 Hz, 1 H), 7.31 (s, 1






H), 4.91-5.04 (m, 1 H), 4.01 (s, 3 H),






3.98 (s, 3 H), 3.32-3.43 (m, 1 H), 3.15-






3.24 (m, 1 H), 2.44 (d, J = 5.02 Hz, 3 H),






2.10-2.23 (m, 1 H), 1.70-1.94 (m, 3 H)


21
3-{[6,7-bis(methyloxy)-4-
2.14a
512.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


10.85 (br. s., 1 H), 8.73 (br. s., 2 H), 8.01-



methyl-4-[(2R)-2-


8.10 (m, 1 H), 7.87 (d, J = 9.03 Hz, 1 H),



(trifluoromethyl)-1-


7.71 (br. s., 1 H), 7.60 (dd, J = 8.78, 2.01



pyrrolidinyl]benzenesulfon-


Hz, 1 H), 7.52 (d, J = 8.78 Hz, 1 H), 7.33 (q,



amide trifluoroacetate


J = 4.94 Hz, 1 H), 4.96 (br. s., 1 H), 3.36-






3.47 (m, 1 H), 3.14-3.25 (m, 1 H), 2.44






(d, J = 5.02 Hz, 3 H), 2.09-2.23 (m, 1 H),






1.68-1.95 (m, 3H)


22
3-[(6-chloro-4-
2.25a
486.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl)amino]-N-


11.53 (br. s., 1 H), 8.89 (s, 1 H), 8.34 (s, 1



methyl-4-[(2R)-2-


H), 8.06-8.21 (m, 2 H), 7.89 (q, J = 4.77



(trifluoromethyl)-1-pyrro-


Hz, 1 H), 7.61 (t, J = 9.29 Hz, 1 H), 7.37 (s,



lidinyl]benzenesulfon-


1 H), 4.04 (s, 3 H), 4.01 (s, 3 H), 2.57 (d,



amide trifluoroacetate


J = 4.77 Hz, 3 H)


23
5-{[6,7-bis(methyloxy)-
1.85a
393.1
1H NMR (400 MHz, DMSO-d6) δ ppm



4-quinazolinyl]amino}-2-fluoro-


10.86 (br. s., 1 H), 8.72 (s, 1 H), 8.05 (d,



N-methylbenzenesulfonamide


J = 2.01 Hz, 1 H), 7.98 (s, 1 H), 7.89 (dd,



hydrochloride


J = 8.66, 2.13 Hz, 1 H), 7.79-7.86 (m, 1






H), 7.73 (q, J = 5.10 Hz, 1 H), 7.29 (s, 1 H),






4.00-4.04 (m, 3 H), 3.97-4.00 (m, 3 H),






2.5 (d, 3H, obscured by DMSO)


24
3-{[6,7-bis(methyloxy)-4-
2.05a
459.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-methyl-


10.72 (br. s., 1 H), 8.79 (s, 1 H), 8.09 (s, 1



4-[(trifluoromethyl)oxy]benzene-


H), 7.92 (d, J = 1.76 Hz, 1 H), 7.69 (dd,



sulfonamide trifluoroacetate


J = 8.53, 2.26 Hz, 1 H), 7.41 (q, J = 5.19 Hz,






1 H), 7.34 (d, J = 8.78 Hz, 1 H), 7.29 (s, 1






H), 4.02 (s, 3 H), 3.98 (s, 3 H), 2.95 (br. s.,






4 H), 2.45 (d, J = 5.02 Hz, 3 H), 1.46 (br. s.,






6 H)


25
3-{[6,7-bis(methyloxy)-
2.16a
458.1
1H NMR (400 MHz, DMSO-d6) δ ppm



4-quinazolinyl]amino}-N-


10.68 (br. s., 1 H), 8.78 (s, 2 H), 8.07 (dd,



methyl-4-(1-piperidinyl)benzene-


J = 8.91, 2.13 Hz, 1 H), 7.95 (d, J = 2.01 Hz,



sulfonamide trifluoroacetate


1 H), 7.89 (d, J = 9.03 Hz, 1 H), 7.67 (dd,






J = 8.41, 2.13 Hz, 1 H), 7.40 (q, J = 4.94 Hz,






1 H), 7.33 (d, J = 8.53 Hz, 1 H), 2.95 (br. s.,






4 H), 2.45 (d, J = 5.02 Hz, 3 H), 1.47 (br. s.,






6 H)


26
3-[(6-chloro-4-
2.30a
432.1
1H NMR (500 MHz, DMSO-d6) δ ppm



quinazolinyl)amino]-N-


10.97 (br. s., 1 H), 8.83 (s, 1 H), 7.97 (br.



methyl-4-(1-piperidinyl)benzene-


s., 1 H), 7.72 (br. s., 1 H), 7.59 (dd,



sulfonamide trifluoroacetate


J = 9.03, 2.20 Hz, 1 H), 7.28 (s, 1 H), 7.18-






7.26 (m, 1 H), 7.15 (d, J = 9.03 Hz, 1 H),






4.01 (s, 3 H), 3.96 (s, 3 H), 3.77-3.84 (m,






2 H), 2.40 (d, J = 4.88 Hz, 3 H), 1.88-2.01






(m, 2 H), 1.56-1.67 (m, 2 H), 1.12 (d,






J = 6.10 Hz, 6 H)


27
3-{[6,7-bis(methyloxy)-
2.21a
472.1
1H NMR (400 MHz, DMSO-d6) δ ppm



4-quinazolinyl]amino}-4-(2,5-


10.96 (br. s., 1 H), 8.83 (s, 1 H), 8.64 (s, 1



dimethyl-1-pyrrolidinyl)-N-


H), 8.09 (dd, J = 8.91, 2.13 Hz, 1 H), 7.89



methylbenzenesulfonamide


(d, J = 9.03 Hz, 1 H), 7.80 (d, J = 2.01 Hz, 1



trifluoroacetate


H), 7.58 (dd, J = 8.91, 2.38 Hz, 1 H), 7.24






(d, J = 4.77 Hz, 1 H), 7.18 (d, J = 9.03 Hz, 1






H), 3.82 (br. s., 2 H), 2.42 (d, J = 4.77 Hz, 3






H), 1.89-2.03 (m, 2 H), 1.57-1.69 (m, 2






H), 1.12 (d, 6 H)


28
3-[(6-chloro-4-
2.38a
446.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl)amino]-4-(2,5-


10.83 (br. s., 1 H), 8.61 (br. s., 1 H), 8.03



dimethyl-1-pyrrolidinyl)-N-


(s, 1 H), 7.96 (d, J = 8.03 Hz, 1 H), 7.72-



methylbenzenesulfonamide


7.80 (m, 2 H), 7.61 (q, J = 4.94 Hz, 1 H),



trifluoroacetate


7.29 (s, 1 H), 4.14 (q, J = 10.21 Hz, 2 H),






3.99 (s, 3 H), 3.99 (s, 3 H), 2.47 (d, J = 5.02






Hz, 3 H)


29
3-{[6,7-bis(methyloxy)-4-
2.08a
489.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.97



quinazolinyl]amino}-N-


(s, 1 H), 8.48 (s, 1 H), 8.39 (t, J = 1.76 Hz,



methyl-4-[(2,2,2-


1 H), 7.94-8.00 (m, 1 H), 7.55-7.65 (m,



trifluoroethyl)thio]benzene-


1 H), 7.45-7.55 (m, 2 H), 6.83 (d, J = 2.26



sulfonamide hydrochloride


Hz, 1 H), 6.75 (d, J = 2.26 Hz, 1 H), 4.10 (s,






3 H), 3.92 (s, 3 H), 2.44-2.49 (m, 3 H)


30
3-{[5,7-bis(methyloxy)-4-
1.86a
375.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


10.79 (s, 1 H), 8.83 (s, 1 H), 8.60 (br. s., 1



methylbenzenesulfonamide


H), 7.73 (dd, J = 8.66, 2.13 Hz, 1 H), 7.48-



hydrochloride


7.60 (m, 2 H), 6.98 (d, J = 2.26 Hz, 1 H),






6.92 (d, J = 2.26 Hz, 1 H), 5.08 (q, J = 8.78






Hz, 2 H), 4.14 (s, 3 H), 3.99 (s, 3 H), 2.47






(d, J = 5.02 Hz, 3 H)


31
3-{[5,7-bis(methyloxy)-
2.08a
473
1H NMR (400 MHz, DMSO-d6) δ ppm



4-quinazolinyl]amino}-N-


10.92 (s, 1 H), 9.30 (d, J = 2.01 Hz, 1 H),



methyl-4-[(2,2,2-tri-


8.57 (s, 1 H), 7.42-7.49 (m, 2 H), 7.40 (q,



fluoroethyl)oxy]benzene-


J = 5.02 Hz, 1 H), 6.85 (d, J = 2.26 Hz, 1 H),



sulfonamide hydrochloride


6.79 (d, J = 2.26 Hz, 1 H), 4.08-4.13 (m, 3






H), 3.93 (s, 3 H), 2.77 (s, 6 H), 2.46 (d,






J = 5.02 Hz, 3 H)


32
3-{[5,7-bis(methyloxy)-4-
2.01a
418.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.87



quinazolinyl]amino}-4-


(br. s., 1 H), 8.57 (s, 1 H), 8.17 (s, 1 H),



(dimethylamino)-N-


7.92 (d, J = 8.82 Hz, 1 H), 7.57-7.64 (m, 1



methylbenzenesulfonamide


H), 7.48-7.56 (m, 2 H), 7.34 (s, 1 H),






4.01 (s, 3 H), 3.85 (s, 3 H), 2.45 (d, J = 4.85






Hz, 3 H)


33
3-(5-chloro-6,7-
0.97b
409.0
1H NMR (400 MHz, DMSO-d6) δ ppm



dimethoxyquinazolin-4-


10.87 (br. s., 1 H), 10.24 (br. s., 1 H), 8.70



ylamino)-N-methylbenzene-


(s, 1 H), 8.10 (br. s., 1 H), 7.73-7.84 (m,



sulfonamide trifluoroacetate


2 H), 7.35-7.48 (m, 2 H), 7.32 (d, J = 2.21






Hz, 1 H), 4.25 (t, J = 5.51 Hz, 2 H), 3.93-






4.04 (m, 5 H), 3.85 (s, 3 H), 3.65-3.74






(m, 2 H), 3.48 (d, J = 6.62 Hz, 2 H), 3.30






(br. s., 2 H), 3.11 (br. s., 2 H), 2.42 (d,






J = 4.85 Hz, 3 H), 2.23-2.34 (m, 2 H)


34
4-methoxy-3-(7-methoxy-6-(3-
0.86b
518.1
1H NMR (400 MHz, DMSO-d6) δ ppm



morpholinopropoxy)quinazolin-


10.93 (br. s., 1 H), 8.78 (s, 1 H), 8.05 (s, 1



4-ylamino)-N-methylbenzene-


H), 7.78 (d, J = 2.01 Hz, 1 H), 7.72 (dd,



sulfonamide bis(trifluoroacetate)


J = 8.66, 2.13 Hz, 1 H), 7.51 (d, J = 8.78 Hz,






1 H), 7.44 (q, J = 5.02 Hz, 1 H), 7.28 (s, 1






H), 4.02 (s, 3 H), 3.98 (s, 3 H), 3.88-3.97






(m, 2 H), 2.96 (s, 3 H), 2.44 (d, J = 5.02 Hz,






3 H)


35
3-{[6,7-bis(methyloxy)-4-
1.94a
486.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


11.02-11.20 (m, 1 H), 8.85 (s, 1 H), 8.79



methyl-4-[methyl (2,2,2-


(br. s., 1 H), 7.61-7.77 (m, 2 H), 7.52 (q,



trifluoroethyl)amino]benzene-


J = 5.02 Hz, 1 H), 6.98 (d, J = 1.76 Hz, 1 H),



sulfonamide trifluoroacetate


6.88 (d, J = 2.26 Hz, 1 H), 4.16 (s, 3 H),






4.07 (q, J = 9.54 Hz, 2 H), 3.99 (s, 3 H),






2.92 (s, 3 H), 2.48 (d, J = 5.02 Hz, 3 H)


36
3-{[5,7-bis(methyloxy)-4-
2.05a
486.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


11.09 (s, 1 H), 8.88 (s, 1 H), 8.78 (br. s., 1



methyl-4-[methyl (2,2,2-tri-


H), 7.69 (dd, J = 8.66, 2.13 Hz, 1 H), 7.47



fluoroethyl)amino]benzene-


(q, J = 4.94 Hz, 1 H), 7.41 (d, J = 8.78 Hz, 1



sulfonamide hydrochloride


H), 6.98 (d, J = 1.76 Hz, 1 H), 6.91 (d,






J = 2.01 Hz, 1 H), 4.18 (s, 3 H), 4.04 (s, 3






H), 3.99 (s, 3 H), 2.45 (d, J = 5.02 Hz, 3 H)


37
3-{[5,7-bis(methyloxy)-4-
1.86a
405.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-methyl-


10.73 (s, 1 H), 8.78 (s, 1 H), 8.21 (br. s., 1



4-(methyloxy)benzenesulfon-


H), 7.78-7.87 (m, 1 H), 7.62-7.73 (m, 2



amide hydrochloride


H), 6.96 (d, J = 2.26 Hz, 1 H), 6.90 (d,






J = 2.26 Hz, 1 H), 4.13 (s, 3 H), 3.99 (s, 3






H), 2.49 (d, J = 5.02 Hz, 3 H)


38
3-{[5,7-bis(methyloxy)-4-
1.84a
393.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-4-fluoro-N-


10.92 (s, 1 H), 8.79 (s, 1 H), 8.41 (br. s., 1



methylbenzenesulfonamide


H), 7.92 (d, J = 8.28 Hz, 1 H), 7.70-7.77



hydrochloride


(m, 2 H), 6.97 (d, J = 2.26 Hz, 1 H), 6.92 (d,






J = 2.26 Hz, 1 H), 4.16 (s, 3 H), 3.99 (s, 3






H), 2.49 (s, 3 H)


39
3-{[5,7-bis(methyloxy)-4-
1.92a
409.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-4-chloro-N-


10.81 (br. s., 1 H), 8.85 (s, 1 H), 8.52 (s, 1



methylbenzenesulfonamide


H), 7.67 (dd, J = 8.41, 2.13 Hz, 1 H), 7.44-



hydrochloride


7.53 (m, 2 H), 7.00 (d, J = 2.01 Hz, 1 H),






6.92 (d, J = 2.01 Hz, 1 H), 4.23 (s, 3 H),






4.00 (s, 3 H), 3.67-3.75 (m, 4 H), 2.95-






3.02 (m, 4 H), 2.46 (d, J = 5.02 Hz, 3 H)


40
3-{[5,7-bis(methyloxy)-4-
1.84a
460.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-methyl-


11.07 (br. s., 1 H), 8.76 (s, 1 H), 8.13 (s, 1



4-(4-morpholinyl)benzenesulfon-


H), 7.78 (d, J = 7.78 Hz, 1 H), 7.74 (q,



amide hydrochloride


J = 4.77 Hz, 1 H), 7.43 (d, J = 12.05 Hz, 1






H), 7.31 (s, 1 H), 4.01 (s, 3 H), 3.99 (s, 3






H), 3.90 (s, 3 H), 2.53 (d, J = 5.27 Hz, 3 H)


41
5-{[6,7-bis(methyloxy)-4-
1.72a
423.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-2-fluoro-


11.19 (br. s., 1 H), 8.76 (s, 1 H), 8.10 (s, 1



N-methyl-4-(methyloxy)benzene-


H), 7.79-7.91 (m, 2 H), 7.50-7.59 (m, 2



sulfonamide hydrochloride


H), 7.34 (s, 1 H), 4.93 (q, J = 8.78 Hz, 2 H),






4.26 (q, J = 6.86 Hz, 2 H), 4.02 (s, 3 H),






2.47 (d, J = 5.02 Hz, 3 H), 1.46 (t, J = 7.03






Hz, 3 H)


42
3-{[6-(ethyloxy)-7-
1.97a
487.1
1H NMR (400 MHz, METHANOL-d4) δ



(methyloxy)-4-


ppm 8.44 (br. s., 2 H), 8.38 (s, 1 H), 8.12



quinazolinyl]amino}-N-


(d, J = 2.21 Hz, 1 H), 7.69 (s, 1 H), 7.63



methyl-4-[(2,2,2-


(dd, J = 8.38, 2.21 Hz, 1 H), 7.27 (d, J = 8.38



trifluoroethyl)oxy]benzene-


Hz, 1 H), 7.20 (s, 1 H), 4.27 (t, J = 5.73 Hz,



sulfonamide hydrochloride


2 H), 4.01 (s, 3 H), 3.77 (t, J = 4.63 Hz, 4






H), 2.79-2.85 (m, 8 H), 2.74 (br. s., 4 H),






2.57 (s, 3 H), 2.13-2.22 (m, 2 H)


43
4-(dimethylamino)-3-(7-
0.89b
531.2
1H NMR (400 MHz, DMSO-d6) δ ppm



methoxy-6-(3-


11.27 (br. s., 1 H), 8.77 (s, 1 H), 8.15 (s, 1



morpholinopropoxy)quinazolin-


H), 7.88 (d, J = 2.26 Hz, 1 H), 7.84 (dd,



4-ylamino)-N-


J = 8.66, 2.38 Hz, 1 H), 7.53-7.59 (m, 2



methylbenzenesulfonamide


H), 7.35 (s, 1 H), 4.84-5.00 (m, 3 H),



bis(formate)


4.01 (s, 3 H), 2.47 (d, J = 5.02 Hz, 3 H),






1.40 (d, J = 6.02 Hz, 6 H)


44
N-methyl-3-{[6-[(1-
2.04a
501.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.58



methylethyl)oxy]-7-


(s, 1 H), 8.19-8.38 (m, 2 H), 7.95 (br. s.,



(methyloxy)-4-


1 H), 7.56 (br. s., 1 H), 7.37 (br. s., 2 H),



quinazolinyl]amino}-4-[(2,2,2-


7.11 (br. s., 2 H), 4.86 (q, J = 8.95 Hz, 2 H),



trifluoroethyl)oxy]benzene-


4.26 (t, J = 5.77 Hz, 2 H), 3.85 (t, J = 6.53



sulfonamide hydrochloride


Hz, 2 H), 2.45 (s, 3 H), 2.25 (quin, J = 6.27






Hz, 2 H)


45
3-({7-[(3-chloropropyl)oxy]-
2.09a
505.0
1H NMR (400 MHz, DMSO-d6) δ ppm



4-quinazolinyl}amino)-N-methyl-


10.18 (s, 1 H), 9.14 (d, J = 2.01 Hz, 1 H),



4-[(2,2,2-


8.53 (s, 1 H), 7.48-7.56 (m, 2 H), 7.45 (q,



trifluoroethyl)oxy]benzene-


J = 4.85 Hz, 1 H), 6.86 (d, J = 2.26 Hz, 1 H),



sulfonamide


6.80 (d, J = 2.51 Hz, 1 H), 5.12 (q, J = 8.95






Hz, 2 H), 4.44 (q, J = 6.94 Hz, 2 H), 3.92 (s,






3 H), 2.47 (d, J = 5.02 Hz, 3 H), 1.46 (t,






J = 6.90 Hz, 3 H)


46
3-{[5-(ethyloxy)-7-
2.08a
487.0
1H NMR (400 MHz, DMSO-d6) δ ppm



(methyloxy)-4-


10.00 (s, 1 H), 8.91 (s, 1 H), 8.48 (s, 1 H),



quinazolinyl]amino}-N-


7.51-7.59 (m, 2 H), 7.44 (q, J = 5.02 Hz, 1



methyl-4-[(2,2,2-


H), 6.84 (d, J = 2.26 Hz, 1 H), 6.81 (d,



trifluoroethyl)oxy]benzene-


J = 2.26 Hz, 1 H), 5.10 (q, J = 8.87 Hz, 2 H),



sulfonamide


5.00 (dt, J = 12.17, 5.96 Hz, 1 H), 3.92 (s, 3






H), 2.48 (d, J = 5.02 Hz, 3 H), 1.44 (d,






J = 6.02 Hz, 6 H)


47
N-methyl-3-{[5-[(1-
2.13a
501.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.59



methylethyl)oxy]-7-


(s, 1 H), 8.40 (s, 1 H), 8.30 (d, J = 9.03 Hz,



(methyloxy)-4-


1 H), 7.94 (d, J = 2.26 Hz, 1 H), 7.69 (dd,



quinazolinyl]amino}-4-


J = 8.78, 2.26 Hz, 1 H), 7.45 (d, J = 8.78 Hz,



[(2,2,2-trifluoro-


2 H), 7.26 (dd, J = 9.16, 2.38 Hz, 1 H), 7.17



ethyl)oxy]benzenesulfonamide


(d, J = 2.51 Hz, 1 H), 4.88 (q, J = 8.78 Hz, 2






H), 4.21 (t, J = 6.27 Hz, 2 H), 3.52 (t,






J = 6.27 Hz, 2 H), 3.28 (s, 3 H), 2.46 (d,






J = 4.52 Hz, 3 H), 2.03 (quin, J = 6.34 Hz, 2






H)


48
N-methyl-4-[(2,2,2-
2.23a
503.1
1H NMR (500 MHz, DMSO-d6) δ ppm



trifluoroethyl)oxy]-3-{[5,6,7-


10.59 (br. s., 1 H), 8.75 (s, 2 H), 7.63-



tris(methyloxy)-4-


7.70 (m, 1 H), 7.52-7.63 (m, 1 H), 7.47



quinazolinyl]amino}benzene-


(q, J = 4.64 Hz, 1 H), 6.93 (s, 1 H), 6.86 (d,



sulfonamide hydrochloride


J = 1.95 Hz, 1 H), 5.49-5.68 (m, 1 H), 4.12






(s, 3 H), 3.97 (s, 3 H), 2.47 (d, J = 4.88 Hz,






3 H), 1.52 (d, J = 6.35 Hz, 3 H)


49
3-{[5,7-bis(methyloxy)-4-
2.08a
487.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


11.14 (s, 1 H), 8.76 (s, 1 H), 8.6 (s, 1 H),



methyl-4-[(2,2,2-trifluoro-1-


8.21 (d, J = 8.28 Hz, 1 H), 7.83-7.93 (m, 2



methylethyl)oxy]benzene-


H), 6.94 (d, J = 2.01 Hz, 1 H), 6.90 (d,



sulfonamide trifluoroacetate


J = 2.01 Hz, 1 H), 4.11 (s, 3 H), 3.99 (s, 3






H), 3.35 (s, 3 H), 2.54 (d, J = 4.77 Hz, 3 H)


50
5-{[5,7-bis(methyloxy)-4-
2.03a
491.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-2-fluoro-


10.81 (br. s., 1 H), 8.77 (s, 2 H), 7.65-



N-methyl-4-[(2,2,2-


7.74 (m, 1 H), 7.39 (d, J = 11.80 Hz, 1 H),



trifluoroethyl)oxy]benzene-


6.92 (d, J = 2.01 Hz, 1 H), 6.83 (d, J = 2.26



sulfonamide trifluoroacetate


Hz, 1 H), 4.15 (s, 3 H), 4.04 (s, 3 H), 3.97






(s, 3 H), 2.52 (br. s., 3 H)


51
5-{[5,7-bis(methyloxy)-4-
1.89a
423.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-2-fluoro-


11.23 (br. s., 1 H), 8.78 (s, 1 H), 8.12 (s, 1



N-methyl-4-(methyloxy)benzene-


H), 7.81-7.88 (m, 2 H), 7.61 (d, J = 11.80



sulfonamide trifluoroacetate


Hz, 1 H), 7.33 (s, 1 H), 4.96 (q, J = 8.78 Hz,






2 H), 4.02 (s, 3 H), 4.00 (s, 3 H), 2.54 (d,






J = 4.77 Hz, 3 H)


52
5-{[6,7-bis(methyloxy)-4-
1.90a
491.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-2-fluoro-


10.80 (s, 1 H), 8.74 (s, 1 H), 7.91 (d,



N-methyl-4-[(2,2,2-


J = 1.51 Hz, 1 H), 7.77 (dd, J = 8.41, 1.88



trifluoroethyl)oxy]benzene-


Hz, 1 H), 7.57-7.66 (m, 2 H), 6.94-6.98



sulfonamide hydrochloride


(m, 2 H), 4.14 (s, 3 H), 3.99 (s, 3 H), 2.54






(s, 3 H), 2.47 (d, J = 5.02 Hz, 3 H)


53
3-{[5,7-bis(methyloxy)-4-
1.92a
421.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


10.84 (s, 1 H), 8.84 (s, 1 H), 8.37-8.44



methyl-4-(methylthio)benzene-


(m, 1 H), 7.86-7.91 (m, 1 H), 7.79-7.86



sulfonamide hydrochloride


(m, 2 H), 6.98-7.03 (m, 2 H), 4.14 (s, 3






H), 3.99 (s, 3 H), 2.49-2.53 (m, 3 H)


54
3-{[5,7-bis(methyloxy)-4-
2.06a
459.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-methyl-


10.75 (br. s., 1 H), 8.92 (br. s., 1 H), 8.76



4-[(trifluoromethyl)oxy]benzene-


(s, 1 H), 7.68-7.88 (m, 3 H), 7.17 (s, 1 H),



sulfonamide hydrochloride


4.18 (s, 3 H), 4.03 (s, 3 H), 3.91 (s, 3 H),






2.53 (br. s., 3 H)


55
N-methyl-4-
2.14a
489.0
1H NMR (400 MHz, DMSO-d6) δ ppm



[(trifluoromethyl)oxy]-3-


10.95 (s, 1 H), 8.83 (s, 1 H), 8.20 (d,



{[5,6,7-tris(methyloxy)-4-


J = 7.78 Hz, 1 H), 7.63 (q, J = 4.77 Hz, 1 H),



quinazolinyl]amino}benzene-


7.17 (d, J = 12.55 Hz, 1 H), 6.97 (d, J = 2.01



sulfonamide trifluoroacetate


Hz, 1 H), 6.91 (d, J = 2.26 Hz, 1 H), 4.14 (s,






3 H), 3.98 (s, 3 H), 2.85 (s, 6 H), 2.50 (br.






s., 3 H)


56
5-{[5,7-bis(methyloxy)-4-
1.93a
436.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-4-


11.21 (br. s., 1 H), 8.79 (s, 1 H), 8.22 (s, 1



(dimethylamino)-2-fluoro-N-methyl-


H), 7.50-7.63 (m, 2 H), 7.32 (s, 1 H),



benzenesulfonamide hydrochloride


7.00 (d, J = 13.30 Hz, 1 H), 4.01 (s, 3 H),






3.99 (s, 3 H), 2.85 (s, 6 H), 2.49 (d, J = 4.77






Hz, 3 H)


57
5-{[6,7-bis(methyloxy)-4-
1.78a
436.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-4-


10.69 (s, 1 H), 8.97 (br. s., 1 H), 8.75 (s, 1



(dimethylamino)-2-fluoro-N-methyl-


H), 7.77 (q, J = 4.77 Hz, 1 H), 7.58 (d,



benzenesulfonamide hydrochloride


J = 11.54 Hz, 1 H), 7.13 (s, 1 H), 5.12 (q,






J = 8.70 Hz, 2 H), 4.13 (s, 3 H), 4.02 (s, 3






H), 3.90 (s, 3 H), 2.54 (d, J = 4.77 Hz, 3 H)


58
2-fluoro-N-methyl-4-[(2,2,2-
2.09a
521.1
1H NMR (400 MHz, DMSO-d6) δ ppm



trifluoroethyl)oxy]-5-{[5,6,7-


10.76 (s, 1 H), 9.03 (br. s., 1 H), 8.69 (s, 1



tris(methyloxy)-4-


H), 7.87 (d, J = 8.28 Hz, 1 H), 7.64-7.72



quinazolinyl]amino}benzene-


(m, 1 H), 7.60 (dd, J = 8.28, 1.76 Hz, 1 H),



sulfonamide trifluoroacetate


7.15 (s, 1 H), 4.21 (s, 3 H), 4.02 (s, 3 H),






3.90 (s, 3 H), 2.50 (br. s., 3 H)


59
4-chloro-N-methyl-3-{[5,6,7-
2.01a
439.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.34



tris(methyloxy)-4-


(br. s., 1 H), 8.32 (s, 1 H), 7.97 (d, J = 1.32



quinazolinyl]amino}benzene-


Hz, 1 H), 7.68 (s, 1 H), 7.64 (dd, J = 8.49,



sulfonamide trifluoroacetate


1.87 Hz, 1 H), 7.43 (d, J = 8.60 Hz, 2 H),






7.16 (s, 1 H), 4.74-4.94 (m, 4 H), 3.90 (s,






3 H), 2.44 (s, 3 H), 1.34 (d, J = 5.95 Hz, 6 H)


60
N-methyl-3-[(7-(methyloxy)-
2.04a
517.1
1H NMR (400 MHz, DMSO-d6) δ ppm



5-{[2-(methyloxy)ethyl]oxy}-


11.03 (br. s., 1 H), 10.26 (br. s., 1 H), 8.47



4-quinazolinyl)amino]-4-


(s, 1 H), 7.88 (s, 2 H), 7.71 (dd, J = 8.71,



[(2,2,2-


2.32 Hz, 1 H), 7.38-7.54 (m, 2 H), 7.16



trifluoroethyl)oxy]benzene-


(s, 1 H), 4.87 (q, J = 8.75 Hz, 2 H), 3.93 (s,



sulfonamide hydrochloride


3 H), 2.42 (d, J = 4.85 Hz, 3 H)


61
N-methyl-3-{[7-(methyloxy)-
2.03a
543.1
1H NMR (400 MHz, METHANOL-d4) δ



5-(tetrahydro-2H-pyran-4-


ppm 9.02 (d, J = 2.26 Hz, 1 H), 8.69 (s, 1



yloxy)-4-quinazolinyl]amino}-


H), 7.66 (dd, J = 8.78, 2.26 Hz, 1 H), 7.40



4-[(2,2,2-trifluoroethyl)oxy]ben-


(d, J = 9.03 Hz, 1 H), 7.00 (s, 1 H), 5.32 (dt,



zenesulfonamide


J = 12.74, 6.31 Hz, 1 H), 4.18 (s, 3 H), 4.00






(s, 3 H), 3.89 (s, 3 H), 2.49 (s, 3 H), 1.53






(d, J = 6.27 Hz, 3 H)


62
N-methyl-4-[(2,2,2-trifluoro-
2.14a
517.1
1H NMR (400 MHz, DMSO-d6) δ ppm



1-methylethyl)oxy]-3-{[5,6,7-


10.93 (s, 1 H), 8.61-8.73 (m, 2 H), 8.19



tris(methyloxy)-4-


(d, J = 8.28 Hz, 1 H), 7.75-7.91 (m, 2 H),



quinazolinyl]amino}benzene-


7.16 (s, 1 H), 4.19 (s, 3 H), 4.03 (s, 3 H),



sulfonamide trifluoroacetate


3.89 (s, 3 H), 3.36 (s, 3 H), 2.55 (d, J = 5.02






Hz, 3 H)


63
N-methyl-4-(methylsulfonyl)-
1.94a
483.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.83



3-{[5,6,7-tris(methyloxy)-4-


(br. s., 1 H), 8.71 (br. s., 1 H), 8.48 (s, 1



quinazolinyl]amino}benzene-


H), 7.85-7.93 (m, 1 H), 7.80 (dd, J = 8.66,



sulfonamide trifluoroacetate


1.88 Hz, 1 H), 7.48-7.60 (m, 2 H), 7.24-






7.31 (m, 1 H), 4.92 (q, J = 8.70 Hz, 2 H),






4.30 (t, J = 5.77 Hz, 2 H), 4.02 (br. s., 2 H),






3.68 (br. s., 2 H), 3.51 (br. s., 2 H), 3.34






(br. s., 2 H), 3.15 (br. s., 2 H), 2.47 (d,






J = 5.02 Hz, 3 H), 2.23 (d, J = 9.03 Hz, 2 H)


64
3-{[5-(ethyloxy)-7-
2.15a
473.1
1H NMR (400 MHz, DMSO-d6) δ ppm



(methyloxy)-4-


10.16 (s, 1 H), 9.04 (d, J = 2.26 Hz, 1 H),



quinazolinyl]amino}-N-


8.53 (s, 1 H), 7.76 (dd, J = 8.53, 1.51 Hz, 1



methyl-4-


H), 7.68 (br. s., 1 H), 7.63 (dd, J = 8.66,



[(trifluoromethyl)oxy]benzene-


2.38 Hz, 1 H), 6.88 (d, J = 2.26 Hz, 1 H),



sulfonamide


6.85 (d, J = 2.26 Hz, 1 H), 5.05 (dt,






J = 12.05, 6.02 Hz, 1 H), 3.93 (s, 3 H), 2.52






(br. s., 3 H), 1.46 (d, J = 6.27 Hz, 6 H)


65
N-methyl-3-{[5-[(1-
2.21a
487.1
1H NMR (400 MHz, DMSO-d6) δ ppm



methylethyl)oxy]-7-


10.05 (s, 1 H), 9.01 (d, J = 2.01 Hz, 1 H),



(methyloxy)-4-


8.51 (s, 1 H), 7.49-7.60 (m, 2 H), 7.44 (q,



quinazolinyl]amino}-4-


J = 5.02 Hz, 1 H), 6.86 (d, J = 2.26 Hz, 1 H),



[(trifluoromethyl)oxy]benzene-


6.80 (d, J = 2.26 Hz, 1 H), 5.53 (dt,



sulfonamide


J = 12.99, 6.43 Hz, 1 H), 4.44 (q, J = 6.94






Hz, 2 H), 3.92 (s, 3 H), 2.48 (d, J = 5.02 Hz,






3 H), 1.54 (d, J = 6.27 Hz, 3 H), 1.46 (t,






J = 7.03 Hz, 3 H)


66
3-{[5-(ethyloxy)-7-
2.14a
501.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.84



(methyloxy)-4-


(s, 1 H), 8.73 (d, J = 1.76 Hz, 1 H), 8.45 (s,



quinazolinyl]amino}-N-


1 H), 7.50-7.60 (m, 2 H), 7.40-7.49 (m,



methyl-4-[(2,2,2-trifluoro-1-


1 H), 6.84 (d, J = 2.26 Hz, 1 H), 6.80 (d,



methylethyl)oxy]benzene-


J = 2.26 Hz, 1 H), 5.41-5.55 (m, 1 H), 4.92-



sulfonamide


5.02 (m, 1 H), 3.93 (s, 3 H), 2.49 (d,






J = 5.02 Hz, 3 H), 1.52 (d, J = 6.27 Hz, 3 H),






1.44 (dd, J = 6.02, 2.26 Hz, 6 H)


67
N-methyl-3-{[5-[(1-
2.20a
515.1
1H NMR (400 MHz, METHANOL-d4) δ



methylethyl)oxy]-7-


ppm 8.81 (s, 1 H), 8.72 (s, 1 H), 8.35 (d,



(methyloxy)-4-


J = 8.28 Hz, 1 H), 8.02 (dd, J = 8.28, 1.76



quinazolinyl]amino}-4-


Hz, 1 H), 7.78 (s, 1 H), 7.30 (s, 1 H), 4.12



[(2,2,2-trifluoro-1-methyl-


(s, 3 H), 4.10 (s, 3 H), 3.24 (s, 3 H), 2.67



ethyl)oxy]benzenesulfonamide


(s, 3 H)


68
3-{[6,7-bis(methyloxy)-4-
1.78a
453.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.62



quinazolinyl]amino}-N-


(br. s., 1 H), 8.43 (s, 1 H), 8.00 (d, J = 2.01



methyl-4-


Hz, 1 H), 7.78 (s, 1 H), 7.69 (dd, J = 8.78,



(methylsulfonyl)benzenesulfon-


2.26 Hz, 1 H), 7.53 (d, J = 8.78 Hz, 1 H),



amide trifluoroacetate


7.42-7.50 (m, 1 H), 7.22 (s, 1 H), 5.39






(dt, J = 12.80, 6.40 Hz, 1 H), 3.96 (s, 3 H),






3.95 (s, 3 H), 2.47 (d, J = 5.02 Hz, 3 H),






1.36 (d, J = 6.27 Hz, 3 H)


69
3-{[6,7-bis(methyloxy)-4-
1.96a
487.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.45



quinazolinyl]amino}-N-


(br. s., 1 H), 8.44 (s, 1 H), 8.37 (d, J = 9.03



methyl-4-[(2,2,2-trifluoro-1-


Hz, 1 H), 7.90 (d, J = 1.76 Hz, 1 H), 7.54



methylethyl)oxy]benzenesulfon-


(dd, J = 8.53, 2.26 Hz, 1 H), 7.15-7.37 (m,



amide


4 H), 4.20 (t, J = 6.27 Hz, 2 H), 3.52 (t,






J = 6.27 Hz, 2 H), 3.29 (s, 3 H), 2.77 (s, 6






H), 2.43 (s, 3 H), 2.03 (quin, J = 6.27 Hz, 2 H)


70
3-{[6-iodo-7-(methyloxy)-4-
2.06a
568.7
1H NMR (400 MHz, DMSO-d6) δ ppm 9.38



quinazolinyl]amino}-N-


(br. s., 1 H), 8.34 (s, 1 H), 7.97 (d, J = 2.26



methyl-4-[(2,2,2-


Hz, 1 H), 7.72 (s, 1 H), 7.67 (dd, J = 8.66,



trifluoroethyl)oxy]benzene-


2.13 Hz, 1 H), 7.39-7.52 (m, 2 H), 7.17



sulfonamide


(s, 1 H), 4.88 (q, J = 8.95 Hz, 2 H), 4.08-






4.29 (m, 4 H), 2.46 (s, 3 H), 1.43 (q,






J = 7.03 Hz, 6 H)


71
3-{[6,7-bis(ethyloxy)-4-
2.02a
500.9
1H NMR (400 MHz, DMSO-d6) δ ppm 8.91



quinazolinyl]amino}-N-


(d, J = 9.04 Hz, 1 H), 8.77 (s, 1 H), 8.59 (d,



methyl-4-[(2,2,2-trifluoro-


J = 2.26 Hz, 1 H), 8.46 (dd, J = 9.04, 2.26



ethyl)oxy]benzenesulfonamide


Hz, 1 H), 7.77 (d, J = 2.01 Hz, 1 H), 7.63






(dd, J = 8.53, 2.26 Hz, 1 H), 7.34 (q, J = 4.77






Hz, 1 H), 7.24 (d, J = 8.78 Hz, 1 H), 2.79 (s,






6 H), 2.43 (d, J = 4.77 Hz, 3 H)


72
4-(dimethylamino)-N-methyl-
2.05a
403.0
1H NMR (400 MHz, DMSO-d6) δ ppm 9.03



3-((7-nitroquinazolin-4-


(s, 1 H), 8.28 (s, 1 H), 8.02-8.09 (m, 2 H),



yl)amino)benzenesulfonamide


7.50 (dd, J = 8.53, 2.26 Hz, 1 H), 7.30 (q,



hydrochloride


J = 4.68 Hz, 1 H), 7.22 (d, J = 8.53 Hz, 1 H),






6.91 (dd, J = 8.78, 2.26 Hz, 1 H), 6.72 (d,






J = 2.26 Hz, 1 H), 6.07 (s, 2 H), 2.76 (s, 6






H), 2.43 (d, J = 4.77 Hz, 3 H)


73
4-(dimethylamino)-N-methyl-
1.68a
501.2
1H NMR (400 MHz, DMSO-d6) δ ppm



3-[(7-{[3-(4-


11.00 (br. s., 1 H), 8.79 (s, 1 H), 8.15 (s, 1



morpholinyl)propyl]oxy}-4-


H), 7.69 (d, J = 2.26 Hz, 1 H), 7.65 (dd,



quinazolinyl)amino]benzene-


J = 8.78, 2.26 Hz, 1 H), 7.27-7.35 (m, 2



sulfonamide hydrochloride


H), 7.24 (d, J = 8.78 Hz, 1 H), 4.29-4.40






(m, 4 H), 3.75-3.83 (m, 4 H), 3.37 (s, 3






H), 3.37 (s, 3 H), 2.81 (s, 6 H), 2.43 (d,






J = 5.02 Hz, 3 H)


74
N-methyl-3-[(7-{[3-(4-
1.63a
556.1
1H NMR (400 MHz, DMSO-d6) δ ppm



morpholinyl)propyl]oxy}-4-


10.20 (s, 1 H), 9.13 (d, J = 2.26 Hz, 1 H),



quinazolinyl)amino]-4-[(2,2,2-


8.56 (s, 1 H), 7.75 (dd, J = 8.66, 1.63 Hz, 1



trifluoroethyl)oxy]benzene-


H), 7.68 (s, 1 H), 7.62 (dd, J = 8.53, 2.26



sulfonamide trifluoroacetate


Hz, 1 H), 6.89 (d, J = 2.26 Hz, 1 H), 6.83 (d,






J = 2.26 Hz, 1 H), 4.43 (q, J = 7.03 Hz, 2 H),






3.93 (s, 3 H), 2.52 (s, 3 H), 1.50 (t, J = 6.90






Hz, 3 H)


75
N-methyl-3-[(7-{[3-
1.97a
501.1
1H NMR (400 MHz, DMSO-d6) δ ppm



(methyloxy)propyl]oxy}-4-


10.99 (s, 1 H), 8.82-8.92 (m, 2 H), 7.70



quinazolinyl)amino]-4-[(2,2,2-


(dd, J = 8.78, 2.26 Hz, 1 H), 7.50-7.61 (m,



trifluoroethyl)oxy]benzene-


2 H), 7.22 (s, 1 H), 5.12 (q, J = 8.78 Hz, 2



sulfonamide


H), 4.17 (s, 3 H), 4.04 (s, 3 H), 3.92 (s, 3






H), 2.47 (d, J = 4.77 Hz, 3 H)


76
4-(dimethylamino)-N-methyl-
1.38a
446.2
1H NMR (400 MHz, METHANOL-d4) δ



3-[(7-{[3-(methyl-


ppm 9.02 (d, J = 2.01 Hz, 1 H), 8.54 (s, 1



oxy)propyl]oxy}-4-quin-


H), 7.59 (dd, J = 8.78, 2.26 Hz, 1 H), 7.36



azolinyl)amino]benzenesulfon-


(d, J = 8.78 Hz, 1 H), 6.79 (d, J = 2.01 Hz, 1



amide


H), 6.70-6.76 (m, 1 H), 5.28 (dt, J = 12.55,






6.27 Hz, 1 H), 4.08 (s, 3 H), 3.90 (s, 3 H),






2.50 (s, 3 H), 1.52 (d, J = 6.27 Hz, 3 H)


77
3-{[5,7-bis(methyloxy)-4-
1.87a
453.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolinyl]amino}-N-


10.57 (br. s., 1 H), 8.76 (s, 1 H), 8.63 (br.



methyl-4-(methylsulfonyl)benzene-


s., 1 H), 7.79 (q, J = 4.68 Hz, 1 H), 7.59 (d,



sulfonamide trifluoroacetate


J = 11.54 Hz, 1 H), 6.93 (d, J = 2.01 Hz, 1






H), 6.85 (d, J = 2.26 Hz, 1 H), 5.08 (q,






J = 8.78 Hz, 2 H), 4.11 (s, 3 H), 3.98 (s, 3






H), 2.54 (d, J = 4.77 Hz, 3 H)


78
3-(7-hydroxy-6-
0.80b
458.9
1H NMR (400 MHz, DMSO-d6) δ ppm



methoxyquinazolin-4-ylamino)-


10.66 (br. s., 1 H), 8.64 (s, 1 H), 7.91 (br.



N-methyl-4-(2,2,2-trifluoro-


s., 1 H), 7.86 (br. s., 1 H), 7.77 (d, J = 10.58



ethoxy)benzenesulfonamide


Hz, 1 H), 7.45-7.57 (m, 2 H), 7.23-7.32






(m, 1 H), 4.81-4.96 (m, 2 H), 4.17-4.31






(m, 2 H), 3.95 (s, 3 H), 2.44 (d, J = 4.63 Hz,






3 H), 1.42 (t, J = 6.73 Hz, 3 H)


79
3-(7-isopropoxy-6-methoxy-
0.94b
501.0
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolin-4-ylamino)-N-


10.64 (br. s., 1 H), 8.80 (s, 1 H), 8.34 (br.



methyl-4-(2,2,2-trifluoro-


s., 1 H), 7.76 (dd, J = 8.66, 2.13 Hz, 1 H),



ethoxy)-benzenesulfonamide


7.48-7.61 (m, 2 H), 7.09 (d, J = 2.01 Hz, 1






H), 6.91 (d, J = 2.01 Hz, 1 H), 5.05 (q,






J = 8.78 Hz, 2 H), 4.55-4.63 (m, 2 H), 3.98






(s, 3 H), 3.77-3.83 (m, 2 H), 3.23 (s, 3 H),






2.47 (d, J = 5.02 Hz, 3 H), 1.04 (d, J = 6.02






Hz, 3 H)


80
3-(7-ethoxy-6-methoxy-
1.03b
487.1
1H NMR (400 MHz, DMSO-d6) δ ppm



quinazolin-4-ylamino)-N-methyl-


11.17 (br. s., 1 H), 8.75 (s, 1 H), 8.02 (s, 1



4-(2,2,2-trifluoro-ethoxy)-


H), 7.76-7.89 (m, 2 H), 7.51-7.58 (m, 2



benzenesulfonamide


H), 7.39 (s, 2 H), 4.90 (q, J = 8.60 Hz, 2 H),



trifluoroacetate


4.26-4.34 (m, 2 H), 3.97 (s, 3 H), 3.72-






3.78 (m, 2 H), 3.32 (s, 3 H), 2.45 (d,






J = 4.85 Hz, 3 H)


81
3-[6-methoxy-7-(2-methoxy-
1.00b
517.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.91



ethoxy)-quinazolin-4-


(s, 1 H), 8.87 (d, J = 1.76 Hz, 1 H), 8.48 (s,



ylamino]-N-methyl-4-(2,2,2-


1 H), 7.48-7.58 (m, 2 H), 7.44 (q, J = 4.77



trifluoro-ethoxy)-


Hz, 1 H), 6.95 (d, J = 2.26 Hz, 1 H), 6.87 (d,



benzenesulfonamide


J = 2.26 Hz, 1 H), 5.09 (q, J = 8.78 Hz, 2 H),



trifluoroacetate


4.98 (dt, J = 9.54, 5.02 Hz, 1 H), 3.86-3.95






(m, 5 H), 3.45-3.56 (m, 2 H), 2.48 (d,






J = 4.77 Hz, 3 H), 2.09-2.19 (m, 2 H), 1.69-






1.85 (m, 2 H)


82
3-{[5,7-bis(methyloxy)-4-
2.07a
487.1
1H NMR (400 MHz, METHANOL-d4) δ



quinazolinyl]amino}-N-


ppm 9.23 (d, J = 2.26 Hz, 1 H), 8.46 (s, 1



methyl-4-[(2,2,2-trifluoro-1-


H), 7.52 (dd, J = 8.78, 2.26 Hz, 1 H), 7.32



methylethyl)oxy]benzene-


(d, J = 8.78 Hz, 1 H), 6.71 (dd, J = 9.16, 2.13



sulfonamide hydrochloride


Hz, 2 H), 5.27 (dt, J = 12.61, 6.37 Hz, 1 H),






4.04 (s, 3 H), 3.86 (s, 3 H), 2.50 (s, 3 H),






1.53 (d, J = 6.27 Hz, 3 H)


83
3-{[5,7-bis(methyloxy)-4-
2.07a
487.1
1H NMR (400 MHz, DMSO-d6) δ ppm 9.78



quinazolinyl]amino}-N-


(s, 1 H), 8.93 (s, 1 H), 8.42 (s, 1 H), 7.85



methyl-4-[(2,2,2-trifluoro-1-


(d, J = 2.26 Hz, 1 H), 7.70 (dd, J = 8.66, 2.13



methylethyl)oxy]benzene-


Hz, 1 H), 7.45 (d, J = 8.53 Hz, 2 H), 7.21 (s,



sulfonamide hydrochloride


1 H), 4.87 (q, J = 8.78 Hz, 2 H), 4.00 (s, 3






H), 2.46 (d, J = 4.77 Hz, 3 H)


84
3-((6-aminoquinazolin-4-
1.54a
372.9
1H NMR (400 MHz, DMSO-d6) δ ppm 9.05



yl)amino)-4-


(br. s., 1 H), 8.31 (s, 1 H), 8.03-8.11 (m,



(dimethylamino)-N-


2 H), 7.50 (dd, J = 8.66, 1.88 Hz, 1 H), 7.22



methylbenzenesulfonamide


(d, J = 8.53 Hz, 1 H), 6.94 (dd, J = 8.91, 1.88






Hz, 1 H), 6.60-6.69 (m, 1 H), 6.55 (d,






J = 2.01 Hz, 1 H), 2.80 (d, J = 4.77 Hz, 3 H),






2.76 (s, 6 H), 2.43 (s, 3 H)


85
3-((7-aminoquinazolin-4-
1.61a
373.0
1H NMR (400 MHz, DMSO-d6) δ ppm 8.95



yl)amino)-4-


(s, 1 H), 8.46 (d, J = 2.26 Hz, 1 H), 8.33 (s,



(dimethylamino)-N-


1 H), 7.56 (d, J = 8.78 Hz, 1 H), 7.50 (dd,



methylbenzenesulfonamide


J = 8.28, 2.26 Hz, 1 H), 7.29-7.39 (m, 2






H), 7.26 (dd, J = 8.78, 2.26 Hz, 1 H), 7.14






(d, J = 2.26 Hz, 1 H), 5.76 (s, 2 H), 2.79 (s,






6 H), 2.45 (d, J = 5.02 Hz, 3 H)


86
4-(dimethylamino)-3-((6-
1.80a
401.0
1H NMR (400 MHz, DMSO-d6) δ ppm



(dimethylamino)quinazolin-


10.97 (br. s., 1 H), 8.65 (s, 1 H), 7.59-



4-yl)amino)-N-


7.71 (m, 3 H), 7.46 (dd, J = 9.03, 2.26 Hz, 1



methylbenzenesulfonamide


H), 7.38 (s, 1 H), 7.28-7.35 (m, 1 H),



bis(trifluoroacetate)


7.21-7.26 (m, 1 H), 2.85 (s, 3 H), 2.81 (s,






6 H), 2.42 (d, J = 5.27 Hz, 3 H)


87
4-(dimethylamino)-N-methyl-
1.71a
387.0
1H NMR (400 MHz, DMSO-d6) δ ppm



3-((6-(methylamino)quinazolin-


10.77 (br. s., 1 H), 8.78-8.87 (m, 1 H),



4-yl)amino)benzenesulfon-


8.75 (s, 1 H), 8.06 (dd, J = 8.91, 1.88 Hz, 1



amide bis(trifluoroacetate)


H), 7.87 (d, J = 9.04 Hz, 1 H), 7.74 (d,






J = 2.01 Hz, 1 H), 7.63 (dd, J = 8.78, 2.26






Hz, 1 H), 7.32 (q, J = 5.02 Hz, 1 H), 7.23 (d,






J = 8.78 Hz, 1 H), 2.80 (s, 6 H), 2.43 (d,






J = 5.02 Hz, 3 H)


88
4-(dimethylamino)-N-methyl-
1.71a
387.0
1H NMR (400 MHz, DMSO-d6) δ ppm



3-((7-(methylamino)quinazolin-


11.14 (br. s., 1 H), 8.71 (s, 1 H), 7.72-



4-yl)amino)benzenesulfon-


7.77 (m, 1 H), 7.60-7.72 (m, 4 H), 7.33



amide


(q, J = 5.19 Hz, 1 H), 7.24 (d, J = 8.53 Hz, 1






H), 3.13 (s, 6 H), 2.81 (s, 6 H), 2.42 (d,






J = 5.27 Hz, 3 H)





aLCMS Method: Agilent 1100 Series LC/MSD SL or VL using electrospray positive [ES + ve to give M + H+] equipped with a Sunfire C18 5.0 μm column (3.0 mm × 50 mm, i.d.), eluting with 0.05% TFA in water (solvent A) and 0.05% TFA in acetonitrile (solvent B), using the following elution gradient: 10%-100% (solvent B) over 2.5 minutes and holding at 100% for 1.7 minutes at a flow rate of 1.0 ml/minutes.


bLCMS Method: Agilent 1200 Series LC/MSD VL using electrospray positive [ES + ve to give M + H+] equipped with a shim-pack XR-ODS 2.2 μm column (3.0 mm × 30 mm, 3.0 mm i.d.) eluting with 0.0375% TFA in water (solvent A) and 0.01875% TFA in acetonitrile (solvent B), using the following elution gradient 10-80% (solvent B) over 0.9 minutes and holding at 80% for 0.6 minutes at a flow rate of 1.2 mL/minutes.






Pharmaceutical Compositions

Example A
Tablets are prepared using conventional methods and are formulated as follows:














Ingredient
Amount per tablet




















Compound of Example I
5
mg



Microcrystalline cellulose
100
mg



Lactose
100
mg



Sodium starch glycollate
30
mg



Magnesium stearate
2
mg



Total
237
mg










Example B
Capsules are prepared using conventional methods and are formulated as follows:














Ingredient
Amount per tablet




















Compound of Example 3
15
mg



Dried starch
178
mg



Magnesium stearate
2
mg



Total
195
mg










Biological Assay(s)

Materials: His-MBP-TEV-Full length human TNNI3K (hTNNI3K) was expressed in Baculokinase system and purified from amylase affinity column followed by Superdex200. The fluorescent ligand 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid was used. The preparation of this fluorescent ligand is disclosed in U.S. Provisional Patent Application No. 61/237,815 filed Aug. 28, 2009, the disclosure of which is incorporated by reference herein. The other buffer components, including MgCl2 (Catalog Number M1028), Bis-Tris (Catalog Number B7535), DTT (Catalog Number D9779) and Chaps (Catalog Number C3023) were purchased from Sigma-Aldrich.
Biological Assay Method I:
A fluorescent polarization assay was used to determine does response of compound inhibition on hTNNI3K ATP binding. The binding of 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid to the hTNNI3K ATP binding pocket results in increase of fluorescent polarization and the displacement of 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid by a competitive compound leads to fluorescent polarization decrease.
Solution 1: Ten (10) mL of a 5 nM 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid solution (Solution 1) was prepared by mixing 5 μL of 1M DTT and 80 μL of 10% (w/v) Chaps and 5 μL of a 10 μM 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino) ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid stock solution into 9910 μL buffer (20 mM Tris, 15 mM MgCl2, pH 7.5). (Stock solution: 10 μM solution of 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid in 100% DMSO)
Solution 2 was formed by mixing 53.8 μL of 2.6 μM hTNNI3K with a 6946.2 μL aliquot of Solution 1 (the above 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid solution) to make up a 7 mL of mixture of hTNNI3K and 5-({[2-({[3-({4-[(5-hydroxy-2-methylphenyl)amino]-2-pyrimidinyl}amino)phenyl]carbonyl}amino)ethyl]amino}carbonyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid (Solution 2).
Fifty (50) nL of inhibitors in DMSO (or DMSO controls) were stamped into a 384-well low volume Greiner black plate, followed by addition of 5 μL of Solution 1 to column 18 and 5 μL Solution 2 to columns 1-17 and 19-24 of the plate. The plate was then spun at 500 rpm for 30 seconds and incubated at room temperature for 60 minutes. After that, the fluorescent polarization was measured on Analyst (ex/em: 485/530 nm, Dichroic: 505). For dose response experiments, normalized data were fit by ABASE/XC50 and pXC50=(log((b−y)/(y−a)))/d−log(x), where x is the compound concentration and y is the % activity at specified compound concentration, a is the minimum % activity, b is the maximum % activity, and d is the Hill slope.
The pXC50s are averaged to determine a mean value, for a minimum of 2 experiments. As determined using the above method, the compounds of Examples 1-88 exhibited a pXC50 greater than or equal to 6.0. Preferred compounds of the invention, including the compounds of Examples 1, 3, 4, 5, 10, 11, 14, 16, 17, 18, 19, 20, 22, 26, 28, 30, 36, 38, 39, 40, 42, 44, 45, 47, 50, 51, 53, 55, 58, 59, 60, 61, 64, 65, 67, 69, 70, 74, 75, 77, 80, 81, 84, 85, 86, 87, and 88, exhibited a pXC50 of between approximately 7.0 and approximately 8.0. For instance, the compound of Example 3 inhibited hTNNI3K in the above method with a mean pXC50 of approximately 7.1. More preferred compounds of the invention, including the compounds of Examples 21, 25, 27, 31, 32, 35, 37, 46, 48, 49, 54, 56, 62, 66, 68, 71, 73, 76, 82, and 83, exhibited a pXC50 of between approximately 8.0 and approximately 9.0. For instance, the compound of Example 68 inhibited hTNNI3K in the above method with a mean pXC50 of approximately 8.4.

## Claims

1. A compound according to Formula I:




wherein:
R1 is (C1-C4)alkyl;
R2 is H or halogen;
R3 is H, halogen, (C1-C8)alkyl, (C1-C8)haloalkyl, hydroxyl, hydroxy(C1-C8)alkyl-, (C1-C8)alkoxy, (C3-C8)cycloalkyloxy, (C1-C8)haloalkoxy, (C1-C8)alkylthio-, (C1-C8)haloalkylthio-, (C3-C8)cycloalkylthio-, (C1-C8)alkylsulfonyl, (C1-C8)haloalkylsulfonyl, (C3-C8)cycloalkylsulfonyl, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb),
wherein said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, (C1-C6)alkoxy, (C1-C4)haloalkoxy, hydroxy(C1-C4)alkyl-, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C6)alkoxy, amino, (C1-C6)alkylamino, ((C1-C6)alkyl)((C1-C6)alkyl)amino, —CO2H, —CO2(C1-C6)alkyl, —CONH2, —CONH(C1-C6)alkyl, or —CON((C1-C6)alkyl)((C1-C6)alkyl), and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C1-C4)alkyl, (C1-C4)haloalkyl, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, hydroxy(C1-C4)alkyl-, oxo, (C1-C4)alkoxy,(C1-C4)haloalkoxy, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc; and
each Rc is, independently, (C1-C8)alkyl or a 5-6 membered heterocycloalkyl, wherein said (C1-C8)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, or —N(Ra)(Rb);
or R6 and R7 taken together represent —O(C1-C2)alkylO—;
or a salt thereof.

2. The compound or salt according to claim 1, wherein R3 is H, halogen, (C1-C6)alkyl, (C1-C6)haloalkyl, (C1-C6)alkoxy, (C5-C6)cycloalkyloxy, (C1-C6)haloalkoxy, (C1-C6)alkylthio-, (C5-C6)cycloalkylthio-, (C1-C6)haloalkylthio-, (C1-C6)alkylsulfonyl, (C1-C6)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb).

3. The compound or salt according to claim 1, wherein R3 is H, halogen, (C1-C8)alkyl, (C1-C8)haloalkyl, hydroxyl, hydroxy(C1-C8)alkyl-, (C1-C8)alkoxy, (C3-C8)cycloalkyloxy, (C1-C8)haloalkoxy, (C1-C8)alkylthio-, (C1-C8)haloalkylthio-, (C3-C8)cycloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, (C1-C6)alkoxy, (C1-C4)haloalkoxy, hydroxy(C1-C4)alkyl-, or —N(Ra)(Rb).

4. The compound or salt according to claim 1, wherein R3 is H, halogen, C1-C6 alkyl, C1-C6 haloalkyl, hydroxyC1-C6 alkyl-, C1-C6 alkoxy, (C3-C6)cycloalkyloxy, C1-C6 haloalkoxy, C1-C6 alkylthio-, (C3-C6)cycloalkylthio-, C1-C6 haloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb), wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb).

5. The compound or salt according to claim 1, wherein R3 is H, halogen, (C1-C4)alkoxy, (C5-C6)cycloalkyloxy, (C1-C4)haloalkoxy, (C1-C4)alkylthio-, (C1-C4)haloalkylthio-, (C5-C6)cycloalkylthio-, (C1-C4)alkylsulfonyl, (C1-C4)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, or —N(Ra)(Rb).

6. The compound or salt according to claim 1, wherein:
each Ra is independently —CH3, —CH2CH3, or —CH2CF3; and
Rb is —CH3;
or Ra and Rb taken together with the nitrogen to which they are attached represent pyrrolidin-1-yl, piperidin-1-yl, or morpholin-4-yl, wherein said pyrrolidin-1-yl, piperidin-1-yl, or morpholin-4-yl is optionally substituted one or two times, independently, by F, —CH3, or —CF3.

7. The compound or salt according to claim 1, wherein:
R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc, and each Rc is, independently, (C1-C4)alkyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl, wherein said (C1-C4)alkyl is optionally substituted by halogen, hydroxyl, trifluoromethyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, pyrrolidinyl, piperidinyl, piperazinyl, or morpholinyl;
or R6 and R7 taken together represent —O(C1-C2)alkylO—.

8. The compound or salt according to claim 1, wherein R6 is H.

9. The compound or salt according to claim 1, wherein:
R6 and R7 are each ORc; and
each Rc is, independently, (C1-C8)alkyl, optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, or ((C1-C4)alkyl)((C1-C4)alkyl)amino.

10. The compound or salt according to claim 1, wherein:
R1 is (C1-C4)alkyl;
R2 is H or halogen;
R3 is H, halogen, (C1-C4)alkoxy, (C5-C6)cycloalkyloxy, (C1-C4)haloalkoxy, (C1-C4)alkylthio-, (C1-C4)haloalkylthio-, (C5-C6)cycloalkylthio-, (C1-C4)alkylsulfonyl, (C1-C4)haloalkylsulfonyl, (C5-C6)cycloalkylsulfonyl, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted one to three times, independently, by halogen, hydroxyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, —CO2H, —CO2(C1-C4)alkyl, —CONH2, —CONH(C1-C4)alkyl, or —CON((C1-C4)alkyl)((C1-C4)alkyl), and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by halogen, (C1-C4)alkyl, (C1-C4)haloalkyl, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, hydroxy(C1-C4)alkyl-, oxo, (C1-C4)alkoxy, (C1-C4)haloalkoxy, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5, R6, and R7 are each, independently, H, halogen, nitro, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, hydroxyl, or ORc; and
each Rc is, independently, (C1-C4)alkyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, piperazinyl, morpholinyl, tetrahydropyranyl, tetrahydro-4H-1,4-thiazinyl, or 1,4-dioxanyl, wherein said (C1-C4)alkyl is optionally substituted by halogen, hydroxyl, trifluoromethyl, (C1-C4)alkoxy, amino, (C1-C4)alkylamino, ((C1-C4)alkyl)((C1-C4)alkyl)amino, pyrrolidinyl, piperidinyl, piperazinyl, or morpholinyl,
or R6 and R7 taken together represent —O(C1-C2)alkylO—.

11. The compound or salt according to claim 1, wherein:
R1 is (C1-C3 alkyl);
R2 is H;
R3 is H, halogen, C1-C6 alkyl, C1-C6 haloalkyl, hydroxyC1-C6 alkyl-, C1-C6 alkoxy, (C3-C6)cycloalkyloxy, C1-C6 haloalkoxy, C1-C6 alkylthio-, (C3-C6)cycloalkylthio-, C1-C6 haloalkylthio-, phenyl, 5-membered heteroaryl, or —N(Ra)(Rb),
wherein said heteroaryl contains one heteroatom selected from N, O and S, or contains one nitrogen atom and optionally contains 1 additional heteroatom selected from N, O and S, or contains two nitrogen atoms and optionally contains 1 additional heteroatom selected from N, O and S; and said phenyl or heteroaryl is optionally substituted one to three times, independently, by halogen, (C1-C6)alkyl, (C1-C4)haloalkyl, or —N(Ra)(Rb);
each Ra is independently (C1-C4)alkyl, wherein said (C1-C4)alkyl is optionally substituted by hydroxyl, trifluoromethyl, (C1-C6)alkoxy, amino, (C1-C6)alkylamino, or ((C1-C6)alkyl)((C1-C6)alkyl)amino, and
Rb is (C1-C4)alkyl;
or Ra and Rb taken together with the nitrogen atom to which they are attached form a 5-membered or 6-membered heterocyclic ring, optionally containing one additional heteroatom selected from nitrogen, oxygen and sulfur, wherein said ring is optionally substituted one or two times, independently, by (C1-C4)alkyl, (C1-C4)haloalkyl, hydroxy(C1-C4)alkyl-, oxo, or (C1-C4)alkoxy(C1-C4)alkyl;
R4 is H;
R5 is H;
R6 and R7 are each ORc and
each Rc is, independently, (C1-C4)alkyl, optionally substituted one to three times, independently, by halogen.

12. A compound selected from:
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(4-morpholinyl)benzenesulfonamide;
3-{[6, 7-bis (methyloxy)-4-quinazolinyl]amino}-4-[ethyl(methyl)amino]-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(1-methylethyl ) oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylthio)benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-(4-quinazolinylamino)benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(dimethylamino)-N -methylbenzenesulfonamide;
4-(dimethylamino)-N-methyl-3-{[6-(methyloxy)-4-quinazolinyl ]amino}benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-[(2,2,2-trifluoroethyl )oxy]benzenesulfonamide;
3-(7,8-dihydro[1,4]dioxino[2,3-g]quinazolin-4-ylamino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(4,4-difluoro-1-piperidinyl)-N-methylbenzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(4,4-difluoro-1-piperidinyl)-N-methylbenzenesulfonamide;
3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-(methyloxy)benzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-[(6,7-bis{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-N-methyl-4-( methylthio)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2R)-2-(trifluoromethyl)-1-pyrrolidinyl]benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-[(2R)-2-(trifluoromethyl)-1-pyrrolidinyl]benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(1-piperidinyl)benzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-N-methyl-4-(1-piperidinyl)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(2,5-dimethyl-1-pyrrolidinyl)-N-methylbenzenesulfonamide;
3-[(6-chloro-4-quinazolinyl)amino]-4-(2,5-dimethyl-1-pyrrolidinyl)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)thio]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-(5-chloro-6,7-dimethoxyquinazolin-4-ylamino)-N-methylbenzenesulfonamide;
4-methoxy-3-(7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-ylamino)-N-methylbenzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[methyl(2,2,2-trifluoroethyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-fluoro-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-chloro-N-methylbenzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(4-morpholinyl)benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-(methyloxy)benzenesulfonamide;
3-{[6-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-3-(7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-ylamino)-N-methylbenzenesulfonamide;
N-methyl-3-{[6-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-({7-[(3-chloropropyl)oxy]-4-quinazolinyl}amino)-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-4-[(2,2,2-trifluoroethyl)oxy]-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-(methyloxy)benzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylthio)benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
N-methyl-4-[(trifluoromethyl)oxy]-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
5-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide;
5-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-4-(dimethylamino)-2-fluoro-N-methylbenzenesulfonamide;
2-fluoro-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]-5-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
4-chloro-N-methyl-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
N-methyl-3-[(7-(methyloxy)-5-{[2-(methyloxy)ethyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-{[7-(methyloxy)-5-(tetrahydro-2H-pyran-4-yloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
N-methyl-4-(methylsulfonyl)-3-{[5,6,7-tris(methyloxy)-4-quinazolinyl]amino}benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(trifluoromethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(trifluoromethyl)oxy]benzenesulfonamide;
3-{[5-(ethyloxy)-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
N-methyl-3-{[5-[(1-methylethyl)oxy]-7-(methyloxy)-4-quinazolinyl]amino}-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide;
3-{[6,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[6-iodo-7-(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
3-{[6,7-bis(ethyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-((7-nitroquinazolin-4-yl)amino)benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide;
N-methyl-3-[(7-{[3-(4-morpholinyl)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]-4-[(2,2,2-trifluoroethyl)oxy]benzenesulfonamide;
4-(dimethylamino)-N-methyl-3-[(7-{[3-(methyloxy)propyl]oxy}-4-quinazolinyl)amino]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-(methylsulfonyl)benzenesulfonamide;
3-(7-hydroxy-6-methoxyquinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoroethoxy)benzenesulfonamide;
3-(7-isopropoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-(7-ethoxy-6-methoxy-quinazolin-4-ylamino)-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-[6-methoxy-7-(2-methoxy-ethoxy)-quinazolin-4-ylamino]-N-methyl-4-(2,2,2-trifluoro-ethoxy)-benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-{[5,7-bis(methyloxy)-4-quinazolinyl]amino}-N-methyl-4-[(2,2,2-trifluoro-1-methylethyl)oxy]benzenesulfonamide;
3-((6-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
3-((7-aminoquinazolin-4-yl)amino)-4-(dimethylamino)-N-methylbenzenesulfonamide;
4-(dimethylamino)-3-((6-(dimethylamino)quinazolin-4-yl)amino)-N-methylbenzenesulfonamide;
4-(dimethylamino)-N-methyl-3-((6-(methylamino)quinazolin-4-yl)amino)benzenesulfonamide; and
4-(dimethylamino)-N-methyl-3-((7-(methylamino)quinazolin-4-yl)amino)benzenesulfonamide;
or a pharmaceutically acceptable salt thereof.

13. A pharmaceutical composition comprising the compound or salt according to claim 1 and a pharmaceutically-acceptable excipient.

14. A method of inhibiting TNNI3K comprising contacting TNNI3K with the compound or salt according to claim 1.

15. A method for treating congestive heart failure comprising administering to a patient in need thereof an effective amount of the compound or salt according to claim 1.

16. A method for treating congestive heart failure comprising administering to a patient in need thereof the pharmaceutical composition according to claim 13.