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                    U.S. DEPARTMENT OF AGRICULTURE
 Methods and Application of Food Composition Laboratory: Beltsville, MD
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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Methods and Application of Food Composition Laboratory People & Locations







Michael R Bukowski

                                                                (PhD)
                        Methods and Application of Food Composition Laboratory

                        




                        
                        RESEARCH CHEMIST
                        Phone: (301) 504-8356
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsFoodomics: New Tools for Food Composition
                                                    In-House Appropriated
                                                                    (D)Accession Number: 445346Farm to Table Factors: Impact of Production, Processing, and Preparation on Food Composition
                                                    In-House Appropriated
                                                                    (D)Accession Number: 445356TeraHerz (THz) Raman and X-Ray Diffraction (XRD) Characterization of Dietary Fiber
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 443517Advanced Technology for Rapid Comprehensive Analysis of the Chemical Components of Foods
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444067Nutrient data on watermelon rind and flesh for publication in the USDA Foundation Foods Database in FoodData Central (FDC)
                                                    Trust Fund Cooperative Agreement
                                                                    (T)Accession Number: 445886**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Metabolomic alteration in mammary glands of pubertal mice fed a high-fat diet
                                                        

- (Peer Reviewed Journal)Yan, L., Rust, B., Sundaram, S., Bukowski, M.R. 2023. Metabolomic alteration in mammary glands of pubertal mice fed a high-fat diet. Nutrition and Metabolic Insights. 16:1-10. https://doi.org/10.1177/11786388221148858.Identification of fecal oncogenic signatures in the inflammatory colon of C57BL/6 mice fed a high fat diet
                                                        

- (Peer Reviewed Journal)Zeng, H., Safratowich, B.D., Cheng, W., Bukowski, M.R. 2022. Identification of fecal oncogenic signatures in the inflammatory colon of C57BL/6 mice fed a high fat diet. Journal of Nutritional Biochemistry. 111. https://doi.org/10.1016/j.jnutbio.2022.109188.Effects of maternal HF diet and absence of TRPC1 gene on mouse placental growth and fetal intrauterine growth retardation (IUGR)
                                                        

- (Peer Reviewed Journal)Larson, K.J., Singh, B., Bundy, A.N., Brunelle, D., Bukowski, M.R., Roemmich, J.N. 2023. Effects of maternal HF diet and absence of TRPC1 gene on mouse placental growth and fetal intrauterine growth retardation (IUGR). Journal of Nutritional Biochemistry. 114. Article 109162. https://doi.org/10.1016/j.jnutbio.2022.109162.Alteration in plasma metabolome in high-fat diet-fed monocyte chemotactic protein-1 knockout mice bearing pulmonary metastasis of Lewis lung carcinoma
                                                        

- (Peer Reviewed Journal)Yan, L., Rust, B.M., Sundaram, S., Picklo, M.J., Bukowski, M.R. 2022. Alteration in plasma metabolome in high-fat diet-fed monocyte chemotactic protein-1 knockout mice bearing pulmonary metastasis of Lewis lung carcinoma. Nutrition and Metabolic Insights. 107. Article 109051. https://doi.org/10.1016/j.jnutbio.2022.109051.Metabolomes of Lewis lung carcinoma metastases and normal lung tissue from mice fed different diets
                                                        

- (Peer Reviewed Journal)Yan, L., Sundaram, S., Rust, B., Picklo, M.J., Bukowski, M.R. 2022. Metabolomes of Lewis lung carcinoma metastases and normal lung tissue from mice fed different diets. Journal of Nutritional Biochemistry. 107. Article 109051. https://doi.org/10.1016/j.jnutbio.2022.109051.Lipidomic analysis of TRPC1 Ca2+ permeable channel-knock out mouse demonstrates a vital role in placental tissue sphingolipid and triacylglycerol homeostasis under maternal high-fat diet
                                                        

- (Peer Reviewed Journal)Bukowski, M.R., Singh, B.B., Roemmich, J.N., Claycombe-Larson, K.J. 2022. Lipidomic analysis of TRPC1 Ca2+ permeable channel-knock out mouse demonstrates a vital role in placental tissue sphingolipid and triacylglycerol homeostasis under maternal high-fat diet. Frontiers in Endocrinology. 13. Article 854269. https://doi.org/10.3389/fendo.2022.854269.Skin carotenoid status of Black or African American college students correlates with plasma carotenoids and fruit and vegetable intake independent of skin tone
                                                        

- (Peer Reviewed Journal)Faraji, B., Bukowski, M.R., Thompson-Johnson, T., Krusinski, L., Lawson Goldgerg, J., Brooks, C.M., Snyder, S. 2022. Skin carotenoid status of Black or African American college students correlates with plasma carotenoids and fruit and vegetable intake independent of skin tone. International Journal of Clinical Nutrition & Dietetics. 8. Article 161. https://doi.org/10.15344/2456-8171/2022/161.Identification of high and low branched-chain fatty acid producing phenotypes in Holstein cows following high forage and low forage diets in a cross-over designed trial1
                                                        

- (Peer Reviewed Journal)Picklo, M.J., Kalscheur, K., Magnuson, A.D., Bukowski, M.R., Harnly, J.M., Fukagawa, N.K., Finley, J.W. 2022. Identification of high and low branched-chain fatty acid producing phenotypes in Holstein cows following high forage and low forage diets in a cross-over designed trial1. Current Developments in Nutrition. Article nzab154. https://doi.org/10.1093/cdn/nzab154.Deriving Information from Complex Data Sets: Impact of Forage on Fatty Acids in Cow Milk
                                                        

- (Peer Reviewed Journal)Harnly, J.M., Picklo, M., Bukowski, M.R., Kalscheur, K., Magnuson, A.D., Fukagawa, N.K., Finley, J.W. 2021. Deriving information from complex data sets: Impact of forage on fatty acids in cow milk. Journal of Food Composition and Analysis. 107:104179. https://doi.org/10.1016/j.jfca.2021.104179.Mammary tumorigenesis and metabolome in adipose specific monocyte chemotactic protein-1 deficient male MMTV-PyMT mice fed a high-fat diet
                                                        

- (Peer Reviewed Journal)Yan, L., Sundaram, S., Rust, B.M., Picklo, M.J., Bukowski, M.R. 2021. Mammary tumorigenesis and metabolome in adipose specific monocyte chemotactic protein-1 deficient male MMTV-PyMT mice fed a high-fat diet. Frontiers in Oncology. 11. Article 667843. https://doi.org/10.3389/fonc.2021.667843.Azoxymethane alters the plasma metabolome to a greater extent in mice fed a high fat diet compared to an AIN-93 diet
                                                        

- (Peer Reviewed Journal)Zeng, H., Umar, S., Liu, Z., Bukowski, M.R. 2021. Azoxymethane alters the plasma metabolome to a greater extent in mice fed a high fat diet compared to an AIN-93 diet. International Journal of Molecular Sciences. https://doi.org/10.3390/metabo11070448.Adequacy of calcium and vitamin D reduces inflammation, beta-catenin signaling and dysbiotic Parasutterela bacteria in the colon of C57BL/6 mice fed a Western-style diet
                                                        

- (Peer Reviewed Journal)Zeng, H., Safratowich, B.D., Liu, Z., Bukowski, M.R., Ishaq, S. 2021. Adequacy of calcium and vitamin D reduces inflammation, beta-catenin signaling and dysbiotic Parasutterela bacteria in the colon of C57BL/6 mice fed a Western-style diet. Journal of Nutritional Biochemistry. 92. Article 108613. https://doi.org/10.1016/j.jnutbio.2021.108613.Identification of phenotypic lipidomic signatures in response to long chain n-3 polyunsaturated fatty acid supplementation in humans
                                                        

- (Peer Reviewed Journal)Picklo, M.J., Vallee Marcotte, B., Bukowski, M.R., Rust, B.M., Guenard, F., Vohl, M. 2021. Identification of phenotypic lipidomic signatures in response to long chain n-3 polyunsaturated fatty acid supplementation in humans. Journal of the American Heart Association. 10. Article 18126. https://doi.org/10.1161/JAHA.120.018126.Simple, rapid lipidomic analysis of triacylglycerols in bovine milk by infusion-electrospray mass spectrometry
                                                        

- (Peer Reviewed Journal)Bukowski, M.R., Picklo, M.J. 2021. Simple, rapid lipidomic analysis of triacylglycerols in bovine milk by infusion-electrospray mass spectrometry. Lipids. 56:243-255. https://doi.org/10.1002/lipd.12292.Time-restricted feeding mice a high-fat diet induces a unique lipidomic profile
                                                        

- (Peer Reviewed Journal)Mehus, A.A., Rust, B.M., Idso, J.P., Hanson, B.K., Zeng, H., Yan, L., Bukowski, M.R., Picklo, M.J. 2020. Time-restricted feeding mice a high-fat diet induces a unique lipidomic profile. Journal of Nutritional Biochemistry. 88. Article 108531. https://doi.org/10.1016/j.jnutbio.2020.108531.The Metabolome of Mammary Tumors Differs from Normal Mammary Glands but Is not Altered by Time-restricted Feeding under Obesogenic Conditions
                                                        

- (Peer Reviewed Journal)Yan, L., Sundaram, S., Rust, B.M., Picklo, M.J., Bukowski, M.R. 2020. The metabolome of mammary tumors differs from normal mammary glands but is not altered by time-restricted feeding under obesogenic conditions. Anticancer Research. https://doi.org/10.21873/anticanres.14358.Advanced liver steatosis accompanies an increase in hepatic inflammation, colonic secondary bile acids and Lactobacillaceae/Lachnospiraceae bacteria in C57BL/6 mice fed a high-fat diet
                                                        

- (Peer Reviewed Journal)Zeng, H., Larson, K.J., Cheng, W., Bukowski, M.R., Safratowich, B.D., Liu, Z., Hakkak, R. 2020. Advanced liver steatosis accompanies an increase in hepatic inflammation, colonic secondary bile acids and lactobacillaceae/lachnospiraceae bacteria in C57BL/6 mice fed a high-fat diet. Journal of Nutritional Biochemistry. https://doi.org/10.1016/j.jnutbio.2019.108336.Concurrent validity of skin carotenoid status as a concentration biomarker of vegetable and fruit intake compared to multiple 24-hour recalls and plasma carotenoid concentrations across one year: a cohort study
                                                        

- (Peer Reviewed Journal)Jahns, L.A., Johnson, L., Conrad, Z.S., Bukowski, M.R., Raatz, S., Pitts, S., Wang, Y., Ermakov, I., Gellermann, W. 2019. Concurrent validity of skin carotenoid status as a concentration biomarker of vegetable and fruit intake compared to multiple 24-hour recalls and plasma carotenoid concentrations across one year: a cohort study. Nutrition Journal. 18:78. https://doi.org/10.1186/s12937-019-0500-0.Dietary saturated fatty acid type impacts obesity-induced metabolic dysfunction and plasma lipidomic signatures in mice
                                                        

- (Peer Reviewed Journal)Zacek, P., Bukowski, M.R., Mehus, A.A., Johnson, L., Zeng, H., Raatz, S.K., Idso, J.P., Picklo, M.J. 2018. Dietary saturated fatty acid type impacts obesity-induced metabolic dysfunction and plasma lipidomic signatures in mice. Journal of Nutritional Biochemistry. https://doi.org/10.1016/j.jnutbio.2018.10.005.Simplified mass spectrometric analysis of ceramides using a common collision energy
                                                        

- (Peer Reviewed Journal)Picklo, M.J., Hanson, B.K., Bukowski, M.R. 2019. Simplified mass spectrometric analysis of ceramides using a common collision energy. Lipids Journal. https://doi.org/10.1002/lipd.12179.Simple and sensitive dilute-and-shoot analysis of carotenoids in human plasma
                                                        

- (Peer Reviewed Journal)Bukowski, M.R., Voeller, K.M., Jahns, L.A. 2018. Simple and sensitive dilute-and-shoot analysis of carotenoids in human plasma. Journal of Chromatography B. 1095:32-38. https://doi.org/10.1016/j.jchromb.2018.07.020.Lipidomic impacts of an obesogenic diet upon Lewis lung carcinoma in mice
                                                        

- (Peer Reviewed Journal)Sundaram, S., Zacek, P., Bukowski, M.R., Mehus, A.A., Yan, L., Picklo, M.J. 2018. Lipidomic impacts of an obesogenic diet upon Lewis lung carcinoma in mice. Frontiers in Oncology. 8:134. https://doi.org/10.3389/fonc.2018.00134.Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry
                                                        

- (Book / Chapter)Bukowski, M.R., Picklo, M.J. 2019. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry. Methods in Molecular Biology. https://doi.org/10.1007/978-1-4939-9187-7_12.Aberrant crypt formation accompanies an increase of opportunistic pathogens/bacteria in the inflammatory gut of C57BL/6 mice fed a high-fat diet

- (Abstract Only)Zeng, H., Ishaq, S.L., Liu, Z., Keehr, K.A., Bukowski, M.R. 2017. Aberrant crypt formation accompanies an increase of opportunistic pathogens/bacteria in the inflammatory gut of C57BL/6 mice fed a high-fat diet [abstract]. Journal of Federation of American Societies for Experimental Biology. 31:435.5.Selective enrichment of n-3 fatty acids in human plasma lipid motifs following intake of marine fish
                                                        

- (Peer Reviewed Journal)Zacek, P., Bukowski, M.R., Johnson, L., Raatz, S.K., Picklo, M.J. 2018. Selective enrichment of n-3 fatty acids in human plasma lipid motifs following intake of marine fish. Journal of Nutritional Biochemistry. 54:57-65. https://doi.org/10.1016/j.jnutbio.2017.11.002.Colonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat diet

- (Peer Reviewed Journal)Zeng, H., Ishaq, S.L., Liu, Z., Bukowski, M.R. 2017. Colonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat diet. Journal of Nutritional Biochemistry. https://doi.org/10.1016/j.jnutbio.2017.11.001.Effects of cooking techniques on fatty acid and oxylipin content of farmed rainbow trout (Oncorhynchus mykiss)
                                                        

- (Peer Reviewed Journal)Flaskerud, K., Bukowski, M.R., Golovko, M., Johnson, L.K., Brose, S., Ali, A., Cleveland, B.M., Picklo, M.J., Raatz, S.K. 2017. Effects of cooking techniques on fatty acid and oxylipin content of farmed rainbow trout (Oncorhynchus mykiss). Food Science and Nutrition. https://doi.org/10.1002/fsn3.512.Dietary saturated fatty acid type modifies the plasma phospholipidome in obese mice

- (Abstract Only)Zacek, P., Bukowski, M.R., Idso, J.P., Johnson, L., Picklo, M.J. 2017. Dietary saturated fatty acid type modifies the plasma phospholipidome in obese mice [abstract]. Keystone Symposia. p.50.Incorporation of dietary n-3 fatty acids into selective phosphatidylcholine lipids in human plasma after salmon intake

- (Abstract Only)Zacek, P., Bukowski, M.R., Raatz, S.K., Picklo, M.J. 2017. Incorporation of dietary n-3 fatty acids into selective phosphatidylcholine lipids in human plasma after salmon intake. Journal of Federation of American Societies for Experimental Biology. 31:42.4.Simpler less expensive method for analysis of inorganic as (iAs) in rice

- (Other)Chaney, R.L., Green, C.E., Lehotay, S.J., Bukowski, M.R. 2016. Simpler less expensive method for analysis of inorganic as (iAs) in rice. Final Project Report to USA Rice Foundation. 30 pp.Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion trap mass spectrometer

- (Peer Reviewed Journal)Zacek, P., Bukowski, M.R., Rosenberger, T., Picklo, M.J. 2016. Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion trap mass spectrometer. Journal of Lipid Research. 57:2225-2234.Effects of frying in various cooking oils on fatty acid content of farmed rainbow trout (Oncorhynchus mykiss)

- (Abstract Only)Flaskerud, K., Picklo, M.J., Bukowski, M.R., Raatz, S.K. 2016. Effects of frying in various cooking oils on fatty acid content of farmed rainbow trout (Oncorhynchus mykiss) [abstract]. Federation of American Societies for Experimental Biology Conference. April 1-6, 2016, San Diego, California. 30:1163.7.Enhanced bioavailability of EPA from emulsified fish oil preparations versus capsular triacylglycerol
                                                        

- (Peer Reviewed Journal)Raatz, S.K., Johnson, L.K., Bibus, D.M., Bukowski, M.R. 2016. Enhanced bioavailability of EPA from emulsified fish oil preparations versus capsular triacylglycerol. Lipids Journal. 51(5):643-651.High-fat diets containing different amounts of n-3 polyunsaturated acids modulate adipokine production in mice
                                                        

- (Peer Reviewed Journal)Sundaram, S., Bukowski, M.R., Lie, W., Picklo, M.J., Yan, L. 2016. High-fat diets containing different amounts of n-3 polyunsaturated acids modulate adipokine production in mice. Lipids. 51(5):571-582.Effects of high-fat diets composed of different oils on adipokine production in mice

- (Abstract Only)Sundaram, S., Bukowski, M.R., Picklo, M.J., Yan, L. 2015. Effects of high-fat diets composed of different oils on adipokine production in mice. Workshop Proceedings. 27.Measuring protein-bound glutathioine (PSSG): Critical correction for cytosolic glutathione species

- (Abstract Only)Bukowski, M.R., Picklo, M.J. 2014. Measuring protein-bound glutathioine (PSSG): Critical correction for cytosolic glutathione species [abstract]. American Society for Mass Spectrometry. p. 58:MP089.Quantitation of protein S-glutathionylation by liquid chromatograph-tandem mass spectrometry: Correction for contaminating glutathione and glutathione disulfide

- (Peer Reviewed Journal)Bukowski, M.R., Bucklin, C., Picklo, M.J. 2015. Quantitation of protein S-glutathionylation by liquid chromatograph-tandem mass spectrometry: Correction for contaminating glutathione and glutathione disulfide. Analytical Biochemistry. 469:54-64.


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