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Ashley  Shepherd    Biological Science Lab Technician (Plants) /ARSUserFiles/49958/cropped.jpg Publications(Clicking on the reprint iconReprint Icon  will take you to the publication reprint.)Methods for cryopreserving of date palm pollen-(Book / Chapter) Araujo De Oliveira, A., Ledo, A., Polek, M., Krueger, R., Shepherd, A.N., Volk, G.M. 2023. Methods for cryopreserving of date palm pollen. In: Rajasekharan, P., Rohini, M., editors. Pollen Cryopreservation Protocols. New York, NY: Humana. p.519-525. https://doi.org/10.1007/978-1-0716-2843-0_49.Citrus micrografting for regrowth after shoot tip cryopreservation-(Book / Chapter) Volk, G.M., Bonnart, R., Shepherd, A. 2020. Citrus micrografting for regrowth after shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-micrografting/.Citrus shoot tip cryopreservation-(Book / Chapter) Volk, G.M., Krueger, R., Moreland, B., Bonnart, R., Shepherd, A. 2020. Citrus shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-cryopreservation/.Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques-(Book / Chapter) Bettoni, J.C., Pathirana, R., Bonnart, R.M., Shepherd, A.N., Volk, G.M. 2019. Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques. In: Faisal, M., Alatar, A., editors. Synthetic Seeds. Switzerland: Springer Nature. p. 469-482. https://doi.org//10.1007/978-3-030-24631-0_22.Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants. Vitis. 58(2):71-78. https://doi.org/10.5073/vitis.2019.58.71-78.Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro. HortScience. 54(6):976-981.Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro-(Peer Reviewed Journal)Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates. CryoLetters. 40(2):103-112.Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants. Acta Horticulturae. 1234:211-218. https://doi.org/10.17660/ActaHortic.2019.1234.28.Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation-(Peer Reviewed Journal) Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Leado, A., Ayala Silva, T. 2019. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Acta Horticulturae. 1234:335-342. https://doi.org/10.17660/ActaHortic.2019.1234.44.Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system-(Peer Reviewed Journal) Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2019. Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system. Acta Horticulturae. 1234:329-334. https://doi.org/10.17660/ActaHortic.2019.1234.43.Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species-(Peer Reviewed Journal) Volk, G.M., Shepherd, A.N., Bonnart, R.M. 2018. Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species. CryoLetters. 39(5):322-330.The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil-(Abstract Only) Bettoni JC, Bonnart R, Shepherd A, Kretzschmar AA, Volk GM. 2018. The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil. Third International Symposium on Plant Cryopreservation. CryoSymp 2018. 26-28 March 2018. Bangkok, Thailand. pg. 24.Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation-(Abstract Only) Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Ledo, A., Ayala Silva, T. 2018. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Meeting Abstract. 57.Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System-(Abstract Only) Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2018. Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System. Meeting Abstract. 20.Citrus cyopreservation: viability of diverse taxa and histological observations-(Peer Reviewed Journal) Volk, G.M., Bonnart, R.M., Shepherd, A.N., Yin, Z., Lee, R.F., Polek, M., Krueger, R. 2016. Citrus cyopreservation: viability of diverse taxa and histological observations. Plant Cell Tissue and Organ Culture. doi:10.1007/211240-016-1112-4. Lab group: Dr. Gayle Volk I develop, improve, and implement cryopreservation techniques for clonally propagated crops and pollen.  Clonal species include: citrus, grape, coffee, walnut, date palm, pecan, hazelnut, and Prunus species.


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