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 Agricultural Genetic Resources Preservation Research: Fort Collins, CO
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Remi  Bonnart    Horticulturist /ARSUserFiles/45186/croppedRB2.jpg Publications(Clicking on the reprint iconReprint Icon  will take you to the publication reprint.)Direct measurement of rice (Oryza sativa) callus cell responses to common molecular cryoprotectants-(Peer Reviewed Journal) Samuels, F., Pearce, K.C., Soderlund, S., Stich, D.G., Bonnart, R.M., Volk, G.M., Levinger, N.E. 2023. Direct measurement of rice (Oryza sativa) callus cell responses to common molecular cryoprotectants. Cell Reports Physical Science. https://doi.org/10.1016/j.xcrp.2023.101469.Tracking permeation of dimethyl sulfoxide (DMSO) in Mentha x piperita shoot tips using coherent Raman microscopy-(Peer Reviewed Journal) Kreckel, H.D., Samuels, F.M., Bonnart, R.M., Volk, G.M., Stich, D., Levinger, N.E. 2023. Tracking permeation of dimethyl sulfoxide (DMSO) in Mentha x piperita shoot tips using coherent Raman microscopy. Plants. 12(12). Article e2247. https://doi.org/10.3390/plants12122247.Visualizing PVS2 component permeation into Oryza sativa callus cells by coherent Raman scattering microscopy-(Abstract Only) Samuels, F.M., Stich, D.G., Bonnart, R.M., Volk, G.M., Levinger, N.E. 2022. Visualizing PVS2 component permeation into Oryza sativa callus cells by coherent Raman scattering microscopy [abstract]. Cryobiology. 109:38. https://doi.org/10.1016/j.cryobiol.2022.11.121.A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agents-(Abstract Only) Kreckel, H.D., Bonnart, R.M., Stich, D., Volk, G.M., Levinger, N.E. 2022. A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agents [abstract]. Cryobiology. 109:14-15. https://doi.org/10.1016/j.cryobiol.2022.11.047.Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation-(Peer Reviewed Journal) Henk, A.D., Bonnart, R.M., Araujo De Oliveira, A., Volk, G.M. 2022. Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation. Applications in Plant Sciences. Article e11489. https://doi.org/10.1002/aps3.11489.Plant tissue culture media preparation-(Book / Chapter) Bonnart, R.M., Chen, K., Volk, G.M. 2022. Plant tissue culture media preparation. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/media/Preparing plant vitrification solution 2-(Book / Chapter) Bonnart, R.M., Chen, K., Volk, G.M. 2022. Preparing plant vitrification solution 2. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/pvs2/Localization of DMSO in Mentha x piperita shoot tips using CARS microscopy-(Abstract Only)Plant cryopreservation: Implementation and outreach-(Peer Reviewed Journal) Volk, G.M., Chen, K., Bonnart, R.M. 2022. Plant cryopreservation: Implementation and outreach. Acta Horticulturae. 1339:93-100. https://doi.org/10.17660/ActaHortic.2022.1339.13.Grapevine shoot tip cryopreservation (droplet vitrification and V-Cryoplate)-(Book / Chapter) Bettoni, J., Bonnart, R.M., Volk, G.M. 2020. Grapevine shoot tip cryopreservation (droplet vitrification and V-Cryoplate). In: Volk, G.M., editor. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/grapevine-shoot-tip-cryopreservation-droplet-vitrification-and-cryoplate/Apple shoot tip cryopreservation-(Book / Chapter) Bettoni, J., Bonnart, R.M., Volk, G.M. 2021. Apple shoot tip cryopreservation. In: Volk, G.M., editor. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/apple-shoot-tip-cryopreservation/Non-uniform distribution of cryoprotecting agents in rice culture cells measured by CARS microscopy-(Peer Reviewed Journal) Samuels, F.M., Stich, D.G., Bonnart, R.M., Volk, G.M., Levinger, N.E. 2021. Non-uniform distribution of cryoprotecting agents in rice culture cells measured by CARS microscopy. Plants. 10(3):589. https://doi.org/10.3390/plants10030589.Considerations for large-scale implementation of dormant budwood cryopreservation-(Peer Reviewed Journal) Tanner, J.D., Chen, K.Y., Bonnart, R.M., Minas, I.S., Volk, G.M. 2021. Considerations for large-scale implementation of dormant budwood cryopreservation. Plant Cell Tissue and Organ Culture. 144:35-48. https://doi.org/10.1007/s11240-020-01884-5.Challenges in implementing plant shoot tip cryopreservation technologies-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Volk, G.M. 2021. Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell Tissue and Organ Culture. 144:21-34. https://doi.org/10.1007/s11240-020-01846-x.Citrus micrografting for regrowth after shoot tip cryopreservation-(Book / Chapter) Volk, G.M., Bonnart, R., Shepherd, A. 2020. Citrus micrografting for regrowth after shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-micrografting/.Prunus shoot tip cryopreservation-(Book / Chapter) Volk, G.M., Bonnart, R. 2020. Prunus shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/prunus-cryopreservation/.Citrus shoot tip cryopreservation-(Book / Chapter) Volk, G.M., Krueger, R., Moreland, B., Bonnart, R., Shepherd, A. 2020. Citrus shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-cryopreservation/.Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques-(Book / Chapter) Bettoni, J.C., Pathirana, R., Bonnart, R.M., Shepherd, A.N., Volk, G.M. 2019. Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques. In: Faisal, M., Alatar, A., editors. Synthetic Seeds. Switzerland: Springer Nature. p. 469-482. https://doi.org//10.1007/978-3-030-24631-0_22.Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants. Vitis. 58(2):71-78. https://doi.org/10.5073/vitis.2019.58.71-78.Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro. HortScience. 54(6):976-981.Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro-(Peer Reviewed Journal)Development of practical and successful Vitis shoot tip cryopreservation protocols-(Abstract Only) Volk G. 2019. Development of practical and successful Vitis shoot tip cryopreservation protocols. Cryobiology 91:194-195.Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates. CryoLetters. 40(2):103-112.Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants-(Peer Reviewed Journal) Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants. Acta Horticulturae. 1234:211-218. https://doi.org/10.17660/ActaHortic.2019.1234.28.Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system-(Peer Reviewed Journal) Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2019. Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system. Acta Horticulturae. 1234:329-334. https://doi.org/10.17660/ActaHortic.2019.1234.43.Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation-(Peer Reviewed Journal) Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Leado, A., Ayala Silva, T. 2019. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Acta Horticulturae. 1234:335-342. https://doi.org/10.17660/ActaHortic.2019.1234.44.Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species-(Peer Reviewed Journal) Volk, G.M., Shepherd, A.N., Bonnart, R.M. 2018. Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species. CryoLetters. 39(5):322-330.The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil-(Abstract Only) Bettoni JC, Bonnart R, Shepherd A, Kretzschmar AA, Volk GM. 2018. The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil. Third International Symposium on Plant Cryopreservation. CryoSymp 2018. 26-28 March 2018. Bangkok, Thailand. pg. 24.Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System-(Abstract Only) Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2018. Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System. Meeting Abstract. 20.Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation-(Abstract Only) Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Ledo, A., Ayala Silva, T. 2018. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Meeting Abstract. 57.Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips-(Peer Reviewed Journal) Gross, B.L., Henk, A.D., Bonnart, R.M., Volk, G.M. 2016. Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips. Plant Cell Reports. 36:459-470. doi:10.1007/s00299-016-2095-7.Citrus cyopreservation: viability of diverse taxa and histological observations-(Peer Reviewed Journal) Volk, G.M., Bonnart, R.M., Shepherd, A.N., Yin, Z., Lee, R.F., Polek, M., Krueger, R. 2016. Citrus cyopreservation: viability of diverse taxa and histological observations. Plant Cell Tissue and Organ Culture. doi:10.1007/211240-016-1112-4.Cryopreservation of citrus for long-term conservation-(Peer Reviewed Journal) Volk, G.M., Bonnart, R.M., Shepherd, A., Krueger, R., Lee, R.F. 2015. Cryopreservation of citrus for long-term conservation. Acta Horticulturae. 1065:187-191.Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting-(Peer Reviewed Journal) Bonnart, R.M., Waddell, J.W., Haiby, K., Widrlechner, M., Volk, G.M. 2014. Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting. CryoLetters. 35(6):507-515.Strategies for improved efficiency when implementing plant vitrification techniques-(Peer Reviewed Journal) Volk, G.M., Shepherd, A., Bonnart, R.M. 2014. Strategies for improved efficiency when implementing plant vitrification techniques. Acta Horticulturae. 1039:85-89.Plant shoot tip response to treatment with plant vitrification solution 2-(Peer Reviewed Journal) Volk, G.M., Henk, A.D., Bonnart, R.M., Shepherd, A., Gross, B.L. 2014. Plant shoot tip response to treatment with plant vitrification solution 2. Acta Horticulturae. 1039:81-84.Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection-(Abstract Only) Bonnart, R.M., Shepherd, A., Lee, R.F., Krueger, R., Volk, G.M. 2013. Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection. Meeting Abstract. 2nd International Symposium on Plant Cryopreservation. p.62.Gene expression in arabidopsis shoot tips after liquid nitrogen exposure-(Abstract Only) Volk, G.M., Henk, A.D., Bonnart, R.M., Gross, B. 2013. Gene expression in arabidopsis shoot tips after liquid nitrogen exposure. Meeting Abstract. 2nd International Society for Horticultural Science Meeting. p.17.Cryopreservation of vitis shoot tips from diverse species-(Abstract Only) Shepherd, A., Bonnart, R.M., Volk, G.M. 2013. Cryopreservation of vitis shoot tips from diverse species. Meeting Abstract. Second International Symposium on Plant Cryopreservation, p.103.Cryopreservation of citrus for long-term conservation-(Abstract Only) Volk, G.M., Bonnart, R.M., Shepherd, A., Krueger, R., Lee, R.F. 2012. Cryopreservation of citrus for long-term conservation. XII International Citrus Congress,  November 18 - 23, 2012, Valencia , Spain. Meeting Abstract. Paper No. S01O02.Conservation of US citrus collections using cryopreservation-(Abstract Only) Volk, G.M., Bonnart, R.M., Krueger, R., Lee, R.F. 2012. Conservation of US citrus collections using cryopreservation. Meeting Abstract. Available: http://ashs.org/abstracts/m/abstracts12/abstract_id_10287.html.Cryopreservation of Citrus shoot tips using micrografting for recovery-(Peer Reviewed Journal) Volk, G.M., Bonnart, R.M., Krueger, R., Lee, R.F. 2012. Cryopreservation of Citrus shoot tips using micrografting for recovery. CryoLetters. 33(6):418-426.Increased efficiency using the encapsulation-dehydration cryopreservation technique-(Peer Reviewed Journal) Bonnart, R.M., Volk, G.M. 2010. Increased efficiency using the encapsulation-dehydration cryopreservation technique. CryoLetters. 31:95-100.Annonaceae seeds: Desiccation tolerant with unusual physiologies-(Abstract Only) Volk, G.M., Bonnart, R.M., Walters, C.T. 2009. Annonaceae seeds: Desiccation tolerant with unusual physiologies. Seed Technologists Newsletter. 83 (2):18-19. Association of Official Seed Analysts and Society for Commercial Seed Technologists. May 29-June 4, 2009. Fort Collins, Colorado. Meeting Abstract.Cryopreservation Of Dormant Buds From Diverse Fraxinus Species-(Peer Reviewed Journal) Volk, G.M., Bonnart, R.M., Waddell, J.W., Widrlechner, M.P. 2009. Cryopreservation Of Dormant Buds From Diverse Fraxinus Species. CryoLetters. 30:262-267.High viability of dormant Malus buds after 10 years of storage in liquid nitrogen vapor-(Peer Reviewed Journal) Volk, G.M., Waddell, J.W., Bonnart, R.M., Towill, L.E., Ellis, D.D., Luffman, M. 2008. High viability of dormant Malus buds after 10 years of storage in liquid nitrogen vapor. CryoLetters 29:89-94.CRYOPRESERVATION OF ARABIDOPSIS THALIANA SHOOT TIPS.-(Peer Reviewed Journal) Towill, L.E., R. Bonnart and G.M. Volk. 2006. Cryopreservation of Arabidopsis thaliana shoot tips. CryoLetters 27:353-360.CRYOPRESERVATION OF APPLE USING NON-DESICCATED SECTIONS FROM WINTER COLLECTED SCIONS-(Peer Reviewed Journal) Towill, L.E. and R.M. Bonnart. 2005. Cryopreservation of apple using non-desiccated sections from winter collected scions. CryoLetters 26:323-332.CRACKING IN A VITRIFICATION SOLUTION DURING COOLING OR WARMING DOES NOT EFFECT GROWTH OF CRYOPRESERVED MINT SHOOT TIPS-(Peer Reviewed Journal) Towill, L.E., Bonnart, R.M. 2003. Cracking in a vitrification solution during cooling or warming does not effect growth of cryopreserved mint shoot tips. CryoLetters. 24:341-346. Lab group: Dr. Gayle Volk I develop cryopreservation protocols for conservation of genetically-diverse vegetatively-propagated agricultural and horticultural plants through applied research. This includes Citrus species and relatives (such as lemons, limes, oranges and kumquats), grapes (Vitis spp.), sweet cherry and peach (Prunus avium and Prunus persica), kiwifruit (Actinidia spp.), and breadfruit (Artocarpus spp.). Other responsibilities and interests include laboratory operation and safety, plant tissue culture media formulation and preparation, and plant propagation and microculture.


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