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                    U.S. DEPARTMENT OF AGRICULTURE
 Methods and Application of Food Composition Laboratory: Beltsville, MD
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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Methods and Application of Food Composition Laboratory People & Locations







Jianghao  Sun
                        Methods and Application of Food Composition Laboratory

                        




                        
                        Research Associate
                        Phone: (301) 504-8466
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsFoodomics: New Tools for Food Composition
                                                    In-House Appropriated
                                                                    (D)Accession Number: 445346Farm to Table Factors: Impact of Production, Processing, and Preparation on Food Composition
                                                    In-House Appropriated
                                                                    (D)Accession Number: 445356High Resolution Mass Spectral Database for Herbs and Foods
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444051Advanced Technology for Rapid Comprehensive Analysis of the Chemical Components of Foods
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444067**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Steroidal glycoalkaloids contribute to anthracnose resistance in solanum lycopersicum
                                                        

- (Peer Reviewed Journal)Fabian, M.L., Zhang, C., Sun, J., Price, N.P., Chen, P., Clarke, C.R., Jones, R.W., Stommel, J.R. 2023. Steroidal glycoalkaloids contribute to anthracnose resistance in solanum lycopersicum. Journal of Experimental Botany. 74(12):3700-3713. https://doi.org/10.1093/jxb/erad108.Chemical composition of honeysuckle (Lonicerae japonicae) extracts and their potential in inhibiting the SARS-CoV-2 spike protein and ACE2 binding, suppressing ACE2, and scavenging radicals
                                                        

- (Peer Reviewed Journal)Gao, B., Zhu, L., Liu, Z., Li, Y., He, X., Wu, X., Pehrsson, P.R., Sun, J., Xie, Z., Slavin, M., Yu, L. 2023. Chemical composition of honeysuckle (Lonicerae japonicae) extracts and their potential in inhibiting the SARS-CoV-2 spike protein and ACE2 binding, suppressing ACE2, and scavenging radicals. Journal of Agricultural and Food Chemistry. 71(15):6133-6143. https://doi.org/10.1021/acs.jafc.3c00584.Chemical characterization of cinnamon (Cinnamomum verum J. Presl) extracts and their capacities in weakening SARS-CoV-2 spike protein binding to ACE2, inhibiting ACE2 and scavenging free radicals
                                                        

- (Peer Reviewed Journal)Xie, Z., Li, Y., Liu, Z., Zeng, M., Moore, J., Gao, B., Wu, X., Sun, J., Wang, T.T., Pehrsson, P.R., He, X., Yu, L. 2023. Chemical characterization of cinnamon (Cinnamomum verum J. Presl) extracts and their capacities in weakening SARS-CoV-2 spike protein binding to ACE2, inhibiting ACE2 and scavenging free radicals. Journal of Agricultural and Food Chemistry. 71:4890-4900. https://doi.org/10.1021/acs.jafc.3c00285.Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals
                                                        

- (Peer Reviewed Journal)Li, Y., Liu, Z., Zeng, M., El Kadiri, A., Huang, J., Kim, A., He, X., Sun, J., Chen, P., Wang, T.T., Zhang, Y., Gao, B., Xie, Z., Yu, L. 2022. Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals. Journal of Agricultural and Food Chemistry. 70(45):14403-14413. https://doi.org/10.1021/acs.jafc.2c06300.Effect of thermal treatments on soluble-free, soluble-conjugated, and insoluble-bound phenolic components and free radical scavenging properties of tomato seeds
                                                        

- (Peer Reviewed Journal)Li, Y., Wu, K., Liu, Z., He, X., Sun, J., Zeng, M., Lee, S., Wang, T.T., Gao, B., Xie, Z., Wei, C., Yu, L. 2022. Effect of thermal treatments on soluble-free, soluble-conjugated, and insoluble-bound phenolic components and free radical scavenging properties of tomato seeds. ACS Food Science and Technology. 2(10):1631-1638. https://doi.org/10.1021/acsfoodscitech.2c00228.Effects of different light-emitting diode illumination on bioactive compounds in mustard “Ruby Streak” microgreens by Ultra-high Performance Liquid Chromatography High-Resolution Mass Spectrometry
                                                        

- (Peer Reviewed Journal)Liu, Z., Teng, Z., Pearlstein, D.J., Chen, P., Yu, L., Zhou, B., Luo, Y., Sun, J. 2022. Effects of different light-emitting diode illumination on bioactive compounds in mustard “Ruby Streak” microgreens by ultra-high performance liquid chromatography high-resolution mass spectrometry. ACS Food Science and Technology. 2(9):1483–1494. https://doi.org/10.1021/acsfoodscitech.2c00193.Identification of soluble free, soluble conjugated and insoluble bound phenolics in tomato seeds and their radical scavenging and antiproliferative activities
                                                        

- (Peer Reviewed Journal)Li, Y., Liu, Z., Tamia, G., He, X., Sun, J., Chen, P., Lee, S., Wang, T.T., Gao, B., Xie, Z., Yu, L. 2022. Identification of soluble free, soluble conjugated and insoluble bound phenolics in tomato seeds and their radical scavenging and antiproliferative activities. Journal of Agricultural and Food Chemistry. 70(29):9039-9047. https://pubs.acs.org/doi/10.1021/acs.jafc.2c03418.Mass Spectrometry Based Non-Targeted and Targeted Analytical Approaches in Fingerprinting and Metabolomics of Food and Agricultral Research
                                                        

- (Review Article)Zhihao, L., Mengliang, Z., Chen, P., Harnly, J.M., Sun, J. 2022. Mass spectrometry based non-targeted and targeted analytical approaches in fingerprinting and metabolomics of food and agricultral research. Journal of Agriculture and Food Chemistry. 70(36):11138–11153. https://doi.org/10.1021/acs.jafc.2c01878.Contrast study on secondary metabolite profile between pastas
made from three single varietal common bean (Phaseolus vulgaris
L.) and durum wheat (Triticum durum)
                                                        

- (Peer Reviewed Journal)Geng, P., Hooper, S., Sun, J., Chen, P., Cichy, K.A., Harnly, J.M. 2022. Contrast study on secondary metabolite profile between pastas made from three single varietal common bean (Phaseolus vulgaris L.) and durum wheat (Triticum durum). ACS Food Science and Technology. 2(5):895–904. https://doi.org/10.1021/acsfoodscitech.2c00050.Profiling of polyphenols and glucosinolates in kale and broccoli microgreens grown under chamber and windowsill conditions by ultrahigh-performance liquid chromatography high-resolution mass spectrometry
                                                        

- (Peer Reviewed Journal)Liu, Z., Shi, J., Wan, J., Pham, Q., Zhang, Z., Sun, J., Yu, L., Luo, Y., Wang, T.T.Y., Chen, P. 2021. Profiling of polyphenols and glucosinolates in kale and broccoli microgreens grown under chamber and windowsill conditions by ultrahigh-performance liquid chromatography high-resolution mass spectrometry. ACS Food Science and Technology. 2(1):101-113. https://doi.org/10.1021/acsfoodscitech.1c00355.Classification of structural characteristics facilitate identifying steroidal saponins inAlliums using ultra-high performance liquid chromatography high-resolution massspectrometry
                                                        

- (Peer Reviewed Journal)Geng, P., Chen, P., Lin, L., Sun, J., Harrington, P., Harnly, J.M. 2021. Classifi cation of structural characteristics facilitate identifying steroidal saponins inAlliums using ultra-high performance liquid chromatography high-resolution massspectrometry . Journal of Food Composition and Analysis. 102:103994. https://doi.org/10.1016/j.jfca.2021.103994.Chemical composition of tomato seed flours, and their radical scavenging, anti-inflammatory and gut microbiota modulating properties
                                                        

- (Peer Reviewed Journal)Choe, U., Sun, J., Bailoni, E., Chen, P., Li, Y., Goa, B., Wang, T.T., Rao, J., Yu, L. 2021. Chemical composition of tomato seed flours, and their radical scavenging, anti-inflammatory and gut microbiota modulating properties. Molecules. 26(5):1478. https://doi.org/10.3390/molecules26051478.Effect of nighttime UV-C irradiation of strawberry plants on phenolic content of fruit: targeted and non-targeted metabolomic analysis
                                                        

- (Peer Reviewed Journal)Sun, J., Janisiewicz, W.J., Takeda, F., Evans, B.E., Jurick Ii, W.M., Zhang, M., Yu, L., Chen, P. 2020. Effect of nighttime UV-C irradiation of strawberry plants on phenolic content of fruit: targeted and non-targeted metabolomic analysis. Journal of Berry Research. 10(3):365-380. https://doi.org/10.3233/JBR-190482.Microgreens of Brassicaceae: Genetic diversity of phytochemical concentrations and antioxidant capacities

- (Peer Reviewed Journal)Xiao, Z., Rausch, S.R., Luo, Y., Sun, J., Yu, L., Wang, Q., Chen, P., Yu, L., Stommel, J.R. 2018. Microgreens of Brassicaceae: Genetic diversity of phytochemical concentrations and antioxidant capacities. LWT - Food Science and Technology. 101:731-737.Challenges of developing a valid Dietary Glucosinolate database
                                                        

- (Peer Reviewed Journal)Wu, X., Sun, J., Haytowitz, D.B., Harnly, J.M., Chen, P., Pehrsson, P.R. 2017. Challenges of developing a valid Dietary Glucosinolate database. Journal of Food Composition and Analysis. https://doi.org/10.1016/j.jfca.2017.07.014.Develop a Comprehensive Flavonoid Analysis Computational Tool for UHPLC-DAD- HRAM-MSn Data

- (Peer Reviewed Journal)Zhang, M., Sun, J., Chen, P. 2017. Develop a Comprehensive Flavonoid Analysis Computational Tool for UHPLC-DAD- HRAM-MSn Data. Analytical Chemist. 89 (4):7388-7397.Botanical supplements: detecting the transition from ingredients to supplements
                                                        

- (Peer Reviewed Journal)Harnly, J.M., Lu, Y., Sun, J., Chen, P. 2017. Botanical supplements: detecting the transition from ingredients to supplements. Journal of Food Composition and Analysis. 64:85-92. https://doi.org/10.1016/j.jfca.2017.06.010.Composition of phenolic compounds in wild apple with multiple resistance mechanisms against postharvest blue mold decay

- (Peer Reviewed Journal)Sun, J., Janisiewicz, W.J., Evans, B.E., Jurick II, W.M., Chen, P. 2017. Composition of phenolic compounds in wild apple with multiple resistance mechanisms against postharvest blue mold decay. Postharvest Biology and Technology. 127:68-75.Feruloyl dopamine-O-hexosides are efficient marker compounds for differentiation between Black Cohosh (Actaea racemosa) and related Actaea species – an UHPLC-HRAM-MS Chemometrics Study
                                                        

- (Peer Reviewed Journal)Geng, P., Harnly, J.M., Sun, J., Zhang, M., Chen, P. 2017. Feruloyl dopamine-O-hexosides are efficient marker compounds for differentiation between Black Cohosh (Actaea racemosa) and related Actaea species – an UHPLC-HRAM-MS Chemometrics Study. Analytical and Bioanalytical Chemistry. 127:68-75. https://doi.org/10.1016/j.postharvbio.2017.01.006.Comprehensive characterization of C-glycosyl flavones in wheat (Triticum aestivum L.) germ using UPLC-PDA-ESI/HRMSn and mass defect filtering

- (Peer Reviewed Journal)Geng, P., Sun, J., Zhang, M., Li, X., Harnly, J.M., Chen, P. 2016. Comprehensive characterization of C-glycosyl flavones in wheat (Triticum aestivum L.) germ using UPLC-PDA-ESI/HRMSn and mass defect filtering. Journal of Mass Spectrometry. 64:4407-4415.GLS-Finder: An Automated Data-Mining System for Fast Profiling Glucosinolates and its Application in Brassica Vegetables

- (Peer Reviewed Journal)Sun, J., Zhang, M., Chen, P. 2016. GLS-Finder: An Automated Data-Mining System for Fast Profiling Glucosinolates and its Application in Brassica Vegetables. Analytical Chemistry. 4407:4415.Myrosinase-dependent and –independent formation and control of isothiocyanate products of glucosinolate hydrolysis

- (Peer Reviewed Journal)Angelino, D., Dosz,.B., Sun, J., Hoeflinger, J.L., Van Tassell, M.L., Chen, P., Harnly, J.M., Miller, M.J., Jeffery, E.H. 2015. Myrosinase-dependent and –independent formation and control of isothiocyanate products of glucosinolate hydrolysis. Frontiers in Plant Science. doi: 10.3389/fpls.2015.00831.FlavonQ: An Automated Data Processing Tool for Profiling Flavone/flavonol Glycosides Using Ultra High-performance Liquid Chromatography Diode Array Detection and High-Resolution Accurate-Mass Mass Spectrometry (UHPLC HRAM-MS)

- (Peer Reviewed Journal)Zhang, M., Sun, J., Chen, P. 2015. FlavonQ: An Automated Data Processing Tool for Profiling Flavone/flavonol Glycosides Using Ultra High-performance Liquid Chromatography Diode Array Detection and High-Resolution Accurate-Mass Mass Spectrometry (UHPLC HRAM-MS). Analytical Chemist. 87:9974-9981.Comparison of Flow Injection MS, NMR, and DNA Sequencing: Methods for Identification and Authentication of Black Cohosh (Actaea racemosa)

- (Peer Reviewed Journal)Harnly, J.M., Chen, P., Sun, J., Huang, H., Colson, K., Yu, J., Mccoy, J.H., Harbaugh Reynaud, D.T., Harrington, P.B., Fletcher, E. 2015. Comparison of Flow Injection MS, NMR, and DNA Sequencing: Methods for Identification and Authentication of Black Cohosh (Actaea racemosa). Planta Medica. 82:250-262.Metabolomic assessment reveals a stimulatory effect of calcium treatment on glucosinolates contents in broccoli microgreen
                                                        

- (Peer Reviewed Journal)Sun, J., Kou, L., Geng, P., Huang, H., Yang, T., Luo, Y., Chen, P. 2015. Metabolomic assessment reveals a stimulatory effect of calcium treatment on glucosinolates contents in broccoli microgreen. Journal of Agricultural and Food Chemistry. 8:63(6):1863-1868.Characterization of phenolic amides from cortex lycii by ultra high-performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry

- (Peer Reviewed Journal)Zhang, J., Guan, S., Sun, J., Liu, T., Chen, P., Feng, R., Chen, X., Hou, J., Yang, W., Wu, W., Yang, M., Guo, D. 2014. Characterization of phenolic amides from cortex lycii by ultra high-performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 407:581-95.High fat diet leads to changes in metabolite patterns in pig plasma, fecal, and urine samples detected by a ultra-high performance liquid chromatography tandem with high resolution mass spectrometry metabolomic study

- (Peer Reviewed Journal)Sun, J., Monagas, M., Jang, S.,  Molokin, A., Harnly, J.M., Urban, J.F., Aguilar, G., Chen, P. 2014. High fat diet leads to changes in metabolite patterns in pig plasma, fecal, and urine samples detected by a ultra-high performance liquid chromatography tandem with high resolution mass spectrometry metabolomic study. Food Chemistry. 15:173:171-178.Profiling of Glucosinolates and Flavonoids in Rorippa indica (Linn.) Hiern. (Cruciferae) by UHPLC-PDA-ESI/HRMSn.

- (Peer Reviewed Journal)Lin, L., Sun, J., Chen, P., Zhang, R., Fan, X., Li, L., Harnly, J.M. 2014. UHPLC-DAD-ESI/HRMSn method to identify the glucosinolates and flavonoids of Rorippa montana (Wall) Small.. Journal of Agricultural and Food Chemistry. 62:6118-6129.
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