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                    U.S. DEPARTMENT OF AGRICULTURE
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Dana N Mitzel

                                                                (PhD)
                        Foreign Arthropod Borne Animal Disease Research

                        




                        
                        Molecular Biologist
                        Phone: (785) 537-5540
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsEnhanced Capacity for Cooperative Research Related to Insect Transmission of Pathogens
                                                    Cooperative Agreement
                                                                    (A)Accession Number: 441111Rift Valley Fever Pathogenesis and Epidemiology
                                                    In-House Appropriated
                                                                    (D)Accession Number: 441467Japanese Encephalitis Virus Prevention and Mitigation Strategies
                                                    In-House Appropriated
                                                                    (D)Accession Number: 441468Development of Diagnostic Tools for Japanese Encephalitis Virus
                                                    Memorandum of Understanding
                                                                    (M)Accession Number: 443051Southeastern USA Arbovirus Landscape
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 443800Assessing the Risk of Emergence, Transmission and Establishment of the Japanese Encephalitis Virus in the United States
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 443942Characterization of Environmental Effects on Mosquito Saliva Composition Using Mass Spectrometry
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444069Vector Surveillance at an Active Cache Valley Virus Focus
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444656Xenosurveillance Using Arthropod Vectors to Characterize Vector-borne Disease Epidemiology
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444667Southwestern USA Arbovirus Landscape
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444680AI/Machine-Learning Software for Measurement of EPG Waveforms from Mosquito Feeding
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444714Host and Vector Transcriptional Responses for Transboundary Arboviral Disease of Livestock
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444719A Genetic Approach to Assess the Spatiotemporal Dynamics of Disease Vectors
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444759Exploration of Novel repRNA Vaccine Candidates for Rift Valley Fever Virus
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 445055Animal Studies in BSL-3 Containment at Pat Roberts Hall for Comparative Zoonotic Arbovirus Studies
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 435561Mammalian Responses to Arboviruses and Sorghum Polyphenols
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 437098Development of a Population Genetics Pipeline for Characterizing Viral and Vector Population Diversity
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 438167Effects of Arbovirus Infection on Insect Feeding Behavior
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 438169Effects of Environmental Temperature on Arbovirus Vector Competence of Various Mosquito Species
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440363Detection and Characterization of Potential Emerging Arboviruses in Brazil
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440427Molecular Characterization of Arbovirus-Mosquito-Vertebrate Host Interactions
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440542Molecular Characterization and Epidemiological Surveillance of Arboviruses in Kenya’s Livestock
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440543Investigating Risk Factors of Rift Valley Fever Virus Direct Transmission in Kenya
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440614Molecular Characterization of Arboviruses in South African Livestock
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440618Characterization of Arbovirus-mosquito-vertebrate Host Interactions
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440631Development of Diagnostic Reagents and Assays for Arbovirus Surveillance
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440694Host Cellular Immune Responses to Arboviruses
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440696Detection and Characterization of Arboviruses and Environmental Influences on Transmission at the U.S. Wildlife and Livestock Interface
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440730Insecticidal Nanoparticles
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 441658Evaluation of Transboundary Disease Mitigation Strategies in Kenya
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442376Investigation of Arboviral Infection Rates and Blood Meal Sources of Various Mosquito Species in Rift Valley Fever Endemic Areas in Kenya
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442633Pathogenesis and Wildlife Host Range of Japanese Encephalitis Virus
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442730A Scalable Vascularized Hydrogel System for Agricultural Vector-borne Disease Research
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442801Assessment of CCHV in Ugandan Cattle Corridor and Kenyan Arid North Tick Populations
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442808Detection and Characterization of Potential Emerging and Emergent Arboviruses in Mosquitoes in the Caribbean
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442817Exosomes as Mediators of Arbovirus Transmission by Insects
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442826Development of Lateral Flow Assays for Rift Valley Fever Virus Antigen and Antibody Detection
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442843Bio-hardened Environments: Integrating AI-based Biothreat Detection Networks into Situational Awareness and Rapid Response Systems
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442870The Impact of Viral Recombination on the Epidemiology of Viruses
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442874Epidemiological Fitness Assessment of Segmented RNA Viruses
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442875Identifying Flavivirus Determinants of Entry and Replication and Design of Potential Vaccine Candidates
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442924Animal and Arthropod Studies in BSL3 Containment at Pat Roberts Hall for Comparative Zoonotic Arbovirus Studies
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 443000Mosquito Cis-regulatory Elements Associated with Arbovirus Infection
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 441171**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Assessing the feasibility, safety, and nutritional quality of using wild-caught pest flies in animal feed

- (Peer Reviewed Journal)Establishment of a Culex tarsalis cell line and its permissiveness to arbovirus infection
                                                        

- (Peer Reviewed Journal)Schirtzinger, E.E., Jasperson, D.C., Swanson, D.A., Mitzel, D.N., Drolet, B.S., Richt, J.A., Wilson, W.C. 2022. Establishment of a Culex tarsalis cell line and its permissiveness to arbovirus infection. Journal of Medical Entomology. 60(1):239-244. https://doi.org/10.1093/jme/tjac155.Susceptibility of sheep to experimental infection with SARS-CoV-2
                                                        

- (Peer Reviewed Journal)Gaudreault, N.N., Cool, K., Trujillo, J.D., Morozov, I., Meekins, D.A., McDowell, C., Bold, D., Carossino, M., Mitzel, D.N., Balaraman, V., Kwon, T., Madden, D.W., Libanori Artiaga, B., Pogranichniy, R.M., Roman-Sosa, G., Henningson, J., Wilson, W.C., Balasuriya, U.B., Garcia-Sastre, A., Richt, J.A. 2022. Susceptibility of sheep to experimental infection with SARS-CoV-2. Emerging Microbes & Infections. 11(1):662-675. https://doi.org/10.1080/22221751.2022.2037397.Infection and transmission of ancestral SARS-CoV-2 and its alpha variant in pregnant white-tailed deer
                                                        

- (Peer Reviewed Journal)Cool, K., Gaudreault, N.N., Morozov, I., Trujillo, J.D., Meekins, D., Mcdowell, C., Carossino, M., Bold, D., Mitzel, D.N., Kwon, T., Balaraman, V., Madden, D.W., Artiaga, B.L., Pogranichniy, R.M., Sosa, G.S., Henningson, J., Wilson, W.C., Balasuriya, U.B., Garcia-Sastre, A., Richt, J.A. 2021. Infection and transmission of ancestral SARS-CoV-2 and its alpha variant in pregnant white-tailed deer. Emerging Microbes & Infections. 11(1):95-112. https://doi.org/10.1080/22221751.2021.2012528.In vitro infection dynamics of Japanese encephalitis virus in established porcine cell lines
                                                        

- (Peer Reviewed Journal)Adetunji, S.A., Smolensky, D., Mitzel, D.N., Chitko-Mckown, C.G., Cernicchiaro, N., Noronha, L.E., Owens, J.L. 2021. In vitro infection dynamics of Japanese encephalitis virus in established porcine cell lines. Pathogens. 10(11). Article 1468. https://doi.org/10.3390/pathogens10111468.Examining the potential transmission of SARS-CoV-2  by insect vectors

- (Abstract Only)Infection dynamics of Japanese encephalitis virus in porcine cell lines

- (Abstract Only)Mechanical transmission of SARS-CoV-2 by house flies
                                                        

- (Peer Reviewed Journal)Balaraman, V., Drolet, B.S., Mitzel, D.N., Wilson, W.C., Owens, J.L., Gaultiero, N.N., Meekins, D.A., Bold, D., Trujillo, J.D., Noronha, L.E., Richt, J.A., Nayduch, D. 2021. Mechanical transmission of SARS-CoV-2 by house flies. Parasites & Vectors. 14:214. https://doi.org/10.1186/s13071-021-04703-8.Porcine macrophage-like cell line C Delta 2+ is susceptible to Japanese encephalitis virus infection

- (Abstract Only)Susceptibility of midge and mosquito vectors to SARS-COV-2 by natural route of infection
                                                        

- (Peer Reviewed Journal)Balaraman, V., Drolet, B.S., Gaudreault, N.N., Wilson, W.C., Owens, J.L., Bold, D., Swanson, D.A., Jasperson, D.C., Noronha, L.E., Richt, J.A., Mitzel, D.N. 2021. Susceptibility of midge and mosquito vectors to SARS-COV-2 by natural route of infection. Journal of Medical Entomology. 58(4):1948-1951. https://doi.org/10.1093/jme/tjab013.Porcine macrophage-like cell line Cdelta2+ is susceptible to Japanese encephalitis virus infection

- (Abstract Only)Research Updates from the Arthropod-Borne Animal Diseases Research Unit

- (Proceedings)Editorial: Emerging arboviruses
                                                        

- (Peer Reviewed Journal)Wilson, W.C., Mitzel, D.N., Savini, G., Zientara, S., Richt, J.A. 2020. Editorial: Emerging arboviruses. Frontiers in Veterinary Science. 7:593872. https://doi.org/10.3389/fvets.2020.593872.Japanese encephalitis virus infects novel Culex cell line

- (Other)Perspectives regarding the risk of introduction of the Japanese encephalitis virus (JEV) in the United States
                                                        

- (Peer Reviewed Journal)Oliveira, A., Cohnstaedt, L.W., Noronha, L.E., Mitzel, D.N., McVey, D.S., Cernicchiaro, N. 2020. Perspectives regarding the risk of introduction of the Japanese encephalitis virus (JEV) in the United States. Frontiers in Veterinary Science.  https://doi.org/10.3389/fvets.2020.00048.


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