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 Cell Wall Biology and Utilization Research: Madison, WI
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ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Cell Wall Biology and Utilization Research People & Locations







Wenli  Li
                        Cell Wall Biology and Utilization Research

                        




                        
                        Research Animal Scientist
                        Phone: (608) 890-0056
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsTargeted Depletion of Cattle Host Transcripts for High-resolution, Metatranscriptomics Analysis of Tissue-embedded Microbial Communities
                                                    Cooperative Agreement
                                                                    (A)Accession Number: 444511Developing Strategies to Improve Dairy Cow Performance and Nutrient Use Efficiency with Nutrition, Genetics, and Microbiology
                                                    In-House Appropriated
                                                                    (D)Accession Number: 442971Using Metatranscriptomics to Investigate the Liver and Blood Microbiome in the Young Calf and Mid-lactation Cows with Feed-induced Acidosis
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 444183Utilizing the University of Wisconsin Institutional Animal Care and Use Committee
                                                    Non-Funded Cooperative Agreement
                                                                    (N)Accession Number: 440145Institutional Biological Safety Committee (IBC) Agreement - University of Wisconsin Madison
                                                    Non-Funded Cooperative Agreement
                                                                    (N)Accession Number: 441643**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Genome-wide copy number variation and structural variation: A novel tool for improved livestock genomic selection

- (Book / Chapter)Liver and muscle transcriptomes differ in mid-lactation cows divergent in feed efficiency in the presence or absence of supplemental rumen-protected choline
                                                        

- (Peer Reviewed Journal)Caputo, M.J.; Li, W.; Kendall, S.J.; Larsen, A.; Weigel, K.A.; White, H.M. Liver and Muscle Transcriptomes Differ in Mid-Lactation Cows Divergent in Feed Efficiency in the Presence or Absence of Supplemental Rumen-Protected Choline. Metabolites 2023, 13, 1023. https://doi.org/10.3390/metabo13091023A meta-analysis of liver microbiome in beef and dairy cattle using metatranscriptome sequencing reads

- (Other)Microbial inoculum alters the rumen epithelial transcriptome and associated meta-transcriptome in calves

- (Abstract Only)Breeding for alfalfa compatibility with corn

- (Proceedings)Riday, H., Grabber, J.H., Li, W., Enjalbert, N., Wagner, S., Mickelson, D. 2022. Breeding for alfalfa compatibility with corn. Proceedings of the World Alfalfa Congress. 11/14/2022 - 11/17/2022.The rumen liquid meta-transcriptome of post-weaned dairy calves differed by pre-weaning ruminal administration of differentially-enriched, rumen-derived inocula
                                                        

- (Peer Reviewed Journal)Park, T., Cersosimo, L.M., Radloff, W.J., Zanton, G.I., Li, W. 2022. The rumen liquid meta-transcriptome of post-weaned dairy calves differed by pre-weaning ruminal administration of differentially-enriched, rumen-derived inocula. Animal Microbiome. 4. Article 4. https://doi.org/10.1186/s42523-021-00142-z.Novel facets of the liver transcriptome are associated with the susceptibility and resistance to lipid-related metabolic disorders in periparturient Holstein cows
                                                        

- (Peer Reviewed Journal)Pralle, R., Li, W., Murphy, B., Holdorf, H.T., White, H. 2021. Novel facets of the liver transcriptome are associated with the susceptibility and resistance to lipid-related metabolic disorders in periparturient Holstein cows. Animals. 11(9). Article 2558. https://doi.org/10.3390/ani11092558.Investigating the effect of positional variation on mid-lactation mammary gland transcriptomics in mice fed either a low-fat or high-fat diet
                                                        

- (Peer Reviewed Journal)Cheng, A., Li, W., Hernandez, L. 2021. Investigating the effect of positional variation on mid-lactation mammary gland transcriptomics in mice fed either a low-fat or high-fat diet. PLoS ONE. 16(8): e0255770. https://doi.org/10.1371/journal.pone.0255770.Effect of natural pre-luteolytic prostaglandin F2a pulses on the bovine luteal transcriptome during spontaneous luteal regression
                                                        

- (Peer Reviewed Journal)Mezera, M., Li, W., Liu, L., Meidan, R., Penagaricano, F., Wiltbank, M. 2021. Effect of natural pre-luteolytic prostaglandin F2a pulses on the bovine luteal transcriptome during spontaneous luteal regression. Biology of Reproduction. 104(5):1016-1029. https://doi.org/10.1093/biolre/ioab123.Analysis of host jejunum transcriptome and associated microbial community structure variation in young calves with feed-induced acidosis
                                                        

- (Peer Reviewed Journal)Naren, G., Li, W., Gelsinger, S., Murphy, B., Li, S. 2021. Analysis of host jejunum transcriptome and associated microbial community structure variation in young calves with feed-induced acidosis. Metabolites. 11(7). Article 414. https://doi.org/10.3390/metabo11070414.The effects of artificially dosed adult rumen contents on abomasum transcriptome and associated microbial community structure in calves
                                                        

- (Peer Reviewed Journal)Narengaowa, F., Li, W., Murphy, B., Cox, M., Suen, G. 2021. The effects of artificially dosed adult rumen contents on abomasum transcriptome and associated microbial community structure in calves. Genes. 12(3). Article 424. https://doi.org/10.3390/genes12030424.Pregnancy-induced changes in the transcriptome of the bovine corpus luteum during and after embryonic interferon-tau secretion
                                                        

- (Peer Reviewed Journal)Mezera, M., Li, W., Meidan, R., Wiltbank, M. 2021. Pregnancy-induced changes in the transcriptome of the bovine corpus luteum during and after embryonic interferon-tau secretion. Biology of Reproduction. 105(1):148-163. https://doi.org/10.1093/biolre/ioab034.Pre-weaning ruminal administration of differentially-enriched, rumen-derived inocula shaped rumen bacterial communities and co-occurrence networks of post-weaned dairy calves
                                                        

- (Peer Reviewed Journal)Park, T., Cersosimo, L.M., Li, W., Radloff, W.J., Zanton, G.I. 2021. Pre-weaning ruminal administration of differentially-enriched, rumen-derived inocula shaped rumen bacterial communities and co-occurrence networks of post-weaned dairy calves. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2021.625488.Investigating the complex interplay between genotype and high-fat-diet feeding in the lactating mammary gland using the Tph1 and Ldlr knockout models
                                                        

- (Peer Reviewed Journal)Cheng, A., Li, W., Walker, T., Silver, C., Ardendt, L., Hernandez, L. 2021. Investigating the complex interplay between genotype and high-fat-diet feeding in the lactating mammary gland using the Tph1 and Ldlr knockout models. American Journal of Physiology. 320(3):E438-E452. https://doi.org/10.1152/ajpendo.00456.2020.Changes in the host transcriptome and microbial metatranscriptome of the ileum of dairy calves subjected to artificial dosing of exogenous rumen contents
                                                        

- (Peer Reviewed Journal)Li, W., Edwards, A., Murphy, B., Cox, M., Raabis, S., Skarlupka, J., Steinberger, A. 2020. Changes in the host transcriptome and microbial metatranscriptome of the ileum of dairy calves subjected to artificial dosing of exogenous rumen contents. Physiological Genomics. 52:333-346. https://doi.org/10.1152/physiolgenomics.00005.2020.De novo assembly of the cattle reference genome with single-molecule sequencing
                                                        

- (Peer Reviewed Journal)Rosen, B.D., Bickhart, D.M., Schnabel, R.D., Koren, S., Elsik, C.G., Tseng, E., Rowan, T.N., Low, W.Y., Zimin, A., Couldrey, C., Hall, R., Li, W., Rhie, A., Ghurye, J., McKay, S.D., Thibaud-Nissen, F., Hoffman, J., Murdoch, B.M., Snelling, W.M., McDaneld, T.G., Hammond, J.A., Schwartz, J.C., Nandolo, W., Hagen, D.E., Dreischer, C., Schultheiss, S.J., Schroeder, S.G., Phillippy, A.M.,Cole, J.B., Van Tassell, C.P., Liu, G., Smith, T.P.L., Medrano, J.F. 2020. De novo assembly of the cattle reference genome with single-molecule sequencing. GigaScience. 9(3):1-9. https://doi.org/10.1093/gigascience/giaa021.Identification of stable genes in the corpus luteum of lactating Holstein cows in pregnancy and luteolysis: implications for selection of RT-qPCR reference genes
                                                        

- (Peer Reviewed Journal)Mezera, M., Li, W., Edwards, A., Koch, D., Beard, A., Wiltbank, M. 2020. Identification of stable genes in the corpus luteum of lactating Holstein cows in pregnancy and luteolysis: implications for selection of RT-qPCR reference genes. Journal of Dairy Science. 103(5):4846-4857. https://doi.org/10.3168/jds.2019-17526.The bovine epimural microbiota displays compositional and structural heterogeneity across different ruminal locations
                                                        

- (Peer Reviewed Journal)Sbardellati, D., Fischer, A., Cox, M.S., Li, W., Kalscheur, K., Suen, G. 2020. The bovine epimural microbiota displays compositional and structural heterogeneity across different ruminal locations. Journal of Dairy Science. 103(4):3636–3647. https://doi.org/10.3168/jds.2019-17649.Changes in meta-transcriptome of rumen epimural microbial community and liver transcriptome in young calves with feed induced acidosis.
                                                        

- (Peer Reviewed Journal)Li, W., Gelsinger, S., Edwards, A., Riehle, C., Koch, D. 2019. Changes in meta-transcriptome of rumen epimural microbial community and liver transcriptome in young calves with feed induced acidosis.. Scientific Reports. 9. Article 18967. https://doi.org/10.1038/s41598-019-54055-8.Identification of single nucleotide polymorphism in Red Clover (Trifolium Pratense L.) using targeted genomic ampliconsequencing and RNA-seq
                                                        

- (Peer Reviewed Journal)Li, W., Riday, H., Riehle, C., Edwards, A., Dinkins, R.D. 2019. Identification of single nucleotide polymorphism in Red Clover (Trifolium Pratense L.) using targeted genomic ampliconsequencing and RNA-seq. Frontiers in Plant Science. 10. Article 1257. https://doi.org/10.3389/fpls.2019.01257.Transcriptome analysis of rumen epithelium and metatranscriptome analysis of rumen epimural microbial community in young calves with feed induced acidosis
                                                        

- (Peer Reviewed Journal)Li, W., Arnold, S., Edwards, A., Riehle, C. 2019. Transcriptome analysis of rumen epithelium and metatranscriptome analysis of rumen epimural microbial community in young calves with feed induced acidosis. Scientific Reports. 9. Article 4744. https://doi.org/10.1038/s41598-019-40375-2.Computational detection and experimental validation of segmental duplications and associated copy number variations in water buffalo (Bubalus bubalis)
                                                        

- (Peer Reviewed Journal)Liu, S., Kang, X., Catacchio, C.R., Liu, M., Fang, L., Schroeder, S.G., Li, W., Rosen, B.D., Iamartino, D., Iannuzzi, L., Sonstegard, T.S., Van Tassell, C.P., Ventura, M., Low, W., Williams, J.L., Bickhart, D.M., Liu, G. 2019. Computational detection and experimental validation of segmental duplications and associated copy number variations in water buffalo (Bubalus bubalis). Functional and Integrative Genomics. 19(3):409–419. https://doi.org/10.1007/s10142-019-00657-4.Effects and immune responses of probiotic treatment in ruminants

- (Literature Review)Transcriptomics analysis of host liver and meta-transcriptome analysis of rumen epimural microbial community in young calves treated with artificial dosing of rumen content from adult donor cow
                                                        

- (Peer Reviewed Journal)Li, W., Edwards, A., Riehle, C., Cox, M., Raabis, S., Steinberger, A., Skarlupka, J., Walling, J.G., Bickhart, D.M., Suen, G. 2019. Transcriptomics analysis of host liver and meta-transcriptome analysis of rumen epimural microbial community in young calves treated with artificial dosing of rumen content from adult donor cow. Scientific Reports. 9. Article 790. https://doi.org/10.1038/s41598-018-37033-4.Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams
                                                        

- (Peer Reviewed Journal)Cheng, A., Hernandez, L., Li, W. 2018. Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams. Physiological Genomics. 50(12):1059-1070. https://doi.org/10.1152/physiolgenomics.00052.2018.Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology
                                                        

- (Peer Reviewed Journal)Choudhary, S., Li, W., Bickhart, D.M., Ramneek, V., Sethi, R.S., Mukhopadhyay, C.S., Choudhary, R.K. 2018. Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology. Journal of Animal Science and Technology. 60. Article 18. https://doi.org/10.1186/s40781-018-0177-5.Genomic structural differences between cattle and river buffalo identified through a combination and genomic and transcriptomic analysis

- (Peer Reviewed Journal)Li, W., Bickhart, D.M., Ramunno, L., Lamartino, D., Williams, J., Liu, G. 2018. Genomic structural differences between cattle and river buffalo identified through a combination and genomic and transcriptomic analysis. Data in Brief. 19:236-239.Comparative sequence alignment reveals River Buffalo genomic structural differences compared with cattle

- (Peer Reviewed Journal)Li, W., Bickhart, D.M., Ramunno, L., Iamartino, D., Williams, J., Liu, G. 2018. Comparative sequence alignment reveals River Buffalo genomic structural differences compared with cattle. Genomics. https://doi.org/10.1016/j.ygeno.2018.02.018.Adverse Maternal Environment Increases Fetal Liver Hydroxymethylation and Alters the Transcriptome

- (Abstract Only)Comparative sequence alignment reveals River Buffalo genomic structural differences compared with cattle

- (Abstract Only)Transcriptome changes in artificial dosing of rumen content in neonatal calves

- (Abstract Only)Effects of xanthosine in isoform switch and splice variants of expressed genes in mammary epithelial cells

- (Abstract Only)Effects of xanthosine on gene expression of mammary epithelial cells using RNA sequencing of goat milk fat globules

- (Abstract Only)


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