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                    U.S. DEPARTMENT OF AGRICULTURE
 Agricultural Genetic Resources Preservation Research: Fort Collins, CO
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ARS Home Plains Area Fort Collins, Colorado Center for Agricultural Resources Research Agricultural Genetic Resources Preservation Research People & Locations







Phil H Purdy
                        Agricultural Genetic Resources Preservation Research

                        




                        
                        Physiologist (Animals)
                        Phone: (970) 492-7512
                        
                        
                        Fax: (970) 492-7505(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsNational Animal Germplasm Program
                                                    In-House Appropriated
                                                                    (D)Accession Number: 442377**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Germplasm preservation for rare domestic animal breeds
                                                        

- (Book / Chapter)Purdy, P.H. 2024. Germplasm preservation for rare domestic animal breeds. In: Skinner, M.K. editor. Encyclopedia of Reproduction. 3rd edition. New York, NY: Elsevier Inc. p. 1-8.Bioclimatic thermal stress indices and their relationships with andrological characteristics in rams
                                                        

- (Peer Reviewed Journal)Molina, J.C., Da Silva, R., Bidegain, F., Souza, Y., Purdy, P.H., Blackburn, H.D., Azevedo, H. 2023. Bioclimatic thermal stress indices and their relationships with andrological characteristics in rams. International Journal of Biometeorology. 67(12):. https://doi.org/10.1007/s00484-023-02587-0.Incorporation of biotechnologies into gene banking strategies to facilitate rapid reconstitution of populations
                                                        

- (Peer Reviewed Journal)Blackburn, H.D., Azevedo, H.C., Purdy, P.H. 2023. Incorporation of biotechnologies into gene banking strategies to facilitate rapid reconstitution of populations. Animals. 13(20),3169. https://doi.org/10.3390/ani13203169.Effects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm
                                                        

- (Peer Reviewed Journal)De Oliveira, M.B., Molina, J.C., Santos Da Silva, R., Ramos, A.F., Purdy, P.H., Azevedo, H.C. 2022. Effects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm. CryoLetters. 43(6):357-367. https://doi.org/10.54680/fr22610110812.Evaluation of boar and bull sperm capacitation and the acrosome reaction by flow cytometry
                                                        

- (Peer Reviewed Journal)Purdy, P.H., Graham, J.K., Azevedo, H.C. 2021. Evaluation of boar and bull sperm capacitation and the acrosome reaction by flow cytometry. Animal Reproduction Science. 246. Article e106846. https://doi.org/10.1016/j.anireprosci.2021.106846.Section 6: Collection and cryopreservation of germplasm and tissues
                                                        

- (Book / Chapter)Blesbois, E., Purdy, P.H., Santiago Moreno, J., Liptói, K., Rajamohan, A., De Souza-Fabjan, J., Somfai, T., Parnpai, R., McGrew, M.J., Mermillod, P., Bailey, J. 2023. Section 6: Collection and cryopreservation of germplasm and tissues. In: Boes, J., Boettcher, P., Honkatukia, M., editors. Innovations in Cryoconservation of Animal Genetic Resources - Practical Guide. FAO Animal and Health Guidelines, No. 33. Rome, Italy. p. 105-157. https://doi.org/10.4060/cc3078en.Section 3: Choice of biological material to be preserved
                                                        

- (Book / Chapter)Purdy, P.H., Blesbois, E., Santiago Moreno, J., Parnpai, R., Smits, K., Somfai, T., Liptói, K., Rajamohan, A., McGrew, M.J., Roelen, B., Várkonyi, E.P., Loi, P., Paiva, S.R., Lessard, C., Zhao, X., Blackburn, H.D., Woelders, H., Bailey, J. 2023. Section 3: Choice of biological material to be preserved. In: Boes, J., Boettcher, P., Honkatukia, M., editors. Innovations in Cryoconservation of Animal Genetic Resources - Practical Guide. FAO Animal and Health Guidelines, No. 33. Rome, Italy. p. 41-68. https://doi.org/10.4060/cc3078en.Critical factors for non-surgical artificial insemination in sheep
                                                        

- (Peer Reviewed Journal)Purdy, P.H., Spiller, S.F., McGuire, E., McGuire, K., Koepke, K., Lake, S., Blackburn, H.D. 2020. Critical factors for non-surgical artificial insemination in sheep. Small Ruminant Research. 191:106179. https://doi.org/10.1016/j.smallrumres.2020.106179.Non-surgical artificial insemination of sheep; successes, challenges and opportunities

- (Proceedings)Purdy, P.H., Spiller, S.F., Blackburn, H.D. 2019. Non-surgical artificial insemination of sheep; successes, challenges and opportunities. Meeting Proceedings, Proceedings of the 27th Technical Conference on Artificial Insemination and Reproduction by the National Association of Animal Breeders, p. 25-29.The effects of gelatin supplementation prior to cooling on ram semen quality and fertility.
                                                        

- (Peer Reviewed Journal)Bandeira, A.M., Matos, J.E., Maria, A.N., Carneiro, P.C., Purdy, P.H., Azevedo, H.C. 2018. The effects of gelatin supplementation prior to cooling on ram semen quality and fertility.. Animal Reproduction(Colégio Brasileiro de Reprodução Animal?). 15(1):23-28. 10.21451/1984-3143-AR2017-0021.Use of anti-phosphotyrosine and PDK1 monoclonal antibodies enables observation of the effects of media on bull sperm second messenger signaling during capacitation.
                                                        

- (Abstract Only)Purdy, P.H., Azevedo, H.C., Graham, J.K., Marquez, M.A. 2018. Use of anti-phosphotyrosine and PDK1 monoclonal antibodies enables observation of the effects of media on bull sperm second messenger signaling during capacitation.. Animal Reproduction Science. 10.1016.The fertility achieved with nonsurgical AI and frozen-thawed ram sperm is influenced by the estrous synchronization protocol, semen cryopreservation diluent, and age and breed of the sheep

- (Abstract Only)Purdy, P.H., Spiller, S.F., Mcguire, E., Mcguire, K., Koepke, K., Stobart, R.H., Burke, J.M., Acharyaf, M., Beemer, T., Moss, G., Lake, S., Blackburn, H.D. 2017. The fertility achieved with nonsurgical AI and frozen-thawed ram sperm is influenced by the estrous synchronization protocol, semen cryopreservation diluent, and age and breed of the sheep. Biology of Reproduction. http://www.ssr.org/sites/ssr.org/files/uploads/attachments/node/482/ssr2017abstracts.pdf.Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding
                                                        

- (Peer Reviewed Journal)Cruz, V.V., Pantel, A., Ray, D.T., Niaura, W., Purdy, P.H., Dierig, D.A. 2017. Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding. Industrial Crops and Products. https://doi.org/10.1016/j.indcrop.2017.07.004.Comparison of effects of cryopreservation diluents on the ability of Ram sperm to reduce resazurin dye

- (Abstract Only)Wonderly, J.L., Koepke, K., Alexander, B., Purdy, P.H. 2017. Comparison of effects of cryopreservation diluents on the ability of Ram sperm to reduce resazurin dye. Research Day Abstracts: Regional Universities Research Day. https://www.ars.usda.gov/research/publications/publication/?seqNo115=340766.Nonsurgical Artificial Insemination in the Ewe

- (Abstract Only)Modification of trout sperm membranes associated with activation and cryopreservation.  Implications for fertilizing potential

- (Peer Reviewed Journal)Purdy, P.H., Barbosa, E.A., Praamsma, C.J., Schisler, G.J. 2016. Modification of trout sperm membranes associated with activation and cryopreservation.  Implications for fertilizing potential. Cryobiology. 73:73-79.Biobanking genetic resources: Challenges and implementation at the USDA National Animal Germplasm Program

- (Peer Reviewed Journal)Purdy, P.H., Wilson, C.S., Spiller, S.F., Blackburn, H.D. 2015. Biobanking genetic resources: Challenges and implementation at the USDA National Animal Germplasm Program. Reproduction, Fertility and Development. 28:1072-1078.Enhance of the estrus and ovulation sychronism in ewes using protocols with progesterone

- (Abstract Only)Nathalia, M.H., Brasil, O.O., Silva, P.C., Saavedra, C.A., Padilha Da Silva, J., Azevedo, H.C., Paiva, S.R., Purdy, P.H., Ramos, A.F. 2015. Enhance of the estrus and ovulation sychronism in ewes using protocols with progesterone. Meeting Abstract. 10o Simpósio de Recursos Genéticos para a América Latina e o Caribe, Oct 26-28, 2015, Bento Gonçalves, RS, Brazil.Utilizing cattle genetic trends to evaluate the robust nature of gene bank collections

- (Abstract Only)Blackburn, H.D., Wilson, C.S., Paiva, S., Spiller, S.F., Purdy, P.H. 2015. Utilizing cattle genetic trends to evaluate the robust nature of gene bank collections. Meeting Abstract. ADSA-ASAS Joint Annual Meeting Orlando, FL July 12-16, 2015.An update on USDA swine gene banking

- (Abstract Only)Purdy, P.H., Blackburn, H.D., Manahan, T.J., Spiller, S.F., Wilson, C.S. 2015. An update on USDA swine gene banking. Meeting Abstract. VIII International Conference on Boar Semen Preservation, Champaign, IL August 9-12, 2015. Vol 50:112-113.Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

- (Peer Reviewed Journal)Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Krisher, R.L., Knox, R.V., Rodriguez-Zas, S.L., Miller, D.J. 2015. Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment. Journal of Andrology. 3:558-568.A preliminary report on the impact of cryopreservation diluent on ram sperm physiology and cervical artificial insemination

- (Proceedings)Purdy, P.H., Spiller, S.F., Rice, S.D., Koeoke, K.L., Mcguire, E.M., Mcguire, K.E., Stobart, S., Blackburn, H.D. 2014. A preliminary report on the impact of cryopreservation diluent on ram sperm physiology and cervical artificial insemination. Symposium Proceedings. Western Section American Society of Animal Science, June 25-27, 2014, San Angelo, Texas.Reproduction

- (Book / Chapter)Inskeep, K., Keisler, D.H., Purdy, P.H. 2016. Reproduction.  Pp. 1000-1044. In: American Sheep Industry Association Inc (eds.). Sheep Production Handbook. Volume 8. Book Chapter. ADS Publishing, Fort Collins, Colorado.Membrane modification strategies for cryopreservation. pp 337-342.
In: Willem F. Wolkers and Harriette Oldenhof (eds.). Cryopreservation and Freeze-Drying Protocls. 3rd ed.The Lab Protocol Series Methods in Molecular Biology.

- (Book / Chapter)Purdy, P.H., Graham, J.K. 2014. Membrane modification strategies for cryopreservation. In: Wolkers, W.F., Oldenhof, H., editors. Cryopreservation and Freeze-Drying Protocls. Third Edition. The Lab Protocol Series Methods in Molecular Biology. Book Chapter. New York: Springer. p. 337-342.Fertility prediction of frozen boar sperm using novel and conventional analyses

- (Abstract Only)Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Rodriguez_Zas, S.L., Krisher, R.L., Knox, R.V., Miller, D.J. 2014. Fertility prediction of frozen boar sperm using novel and conventional analyses. Meeting Abstract. Society for the Study of Reproduction, Grand Rapids, MI, July 19-23, 2014.Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success

- (Peer Reviewed Journal)Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Krisher, R.L., Knox, R.V., Miller, D.J. 2014. Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success. Theriogenology. 82:266-273.Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation

- (Abstract Only)Purdy, P.H., Spiller, S.F., Blackburn, H.D. 2013. Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation. Meeting Abstract. Annual Meeting of the Society for Cryobiology, Bethesda, MD, July 28-31, 2013.Post-thaw motility of frozen boar sperm does not predict success with in vitro fertilization

- (Abstract Only)Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Knox, R.V., Krisher, R.L., Miller, D.J. 2013. Post-thaw motility of frozen boar sperm does not predict success with in vitro fertilization. Meeting Abstract. Society for the Study of Reproduction, Montreal, Quebec, Canada, July 22-26, 2013.Effect of storage time on the viability of cryopreserved bovine spermatozoa

- (Abstract Only)Gallegos, A.I., Ericsson, S.A., Blackburn, H.D., Spiller, S.F., Warnock, B.J., Meador, M.K., Smith, M.W., Purdy, P.H. 2013. Effect of storage time on the viability of cryopreserved bovine spermatozoa. Meeting Abstract. American Society of Animal Science, Indianapolis, IN, July 8-12, 2013.Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation
                                                        

- (Abstract Only)Purdy, P.H., Spiller, S.F., Blackburn, H.D. 2013. Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation. Cryobiology. https://doi.org/10.1016/j.cryobiol.2013.09.035.Current biotechnologies and future possibilities in preservation of animal germplasm

- (Abstract Only)Purdy, P.H. 2013. Current biotechnologies and future possibilities in preservation of animal germplasm. Meeting Abstract. The 2nd International Symposium on Plant Cryopreservation, Fort Collins, CO Aug 11-14, 2013.A simple vitrification method for cryobanking avian testicular tissue

- (Peer Reviewed Journal)Liu, J., Cheng, K.M., Purdy, P.H., Silversides, F.G. 2012. A simple vitrification method for cryobanking avian testicular tissue. Poultry Science. 91:3209-3213.Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster sperm

- (Abstract Only)Tarvis, K.M., Purdy, P.H., Graham, J.K. 2012. Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster sperm. Meeting Abstract. International Congress on Animal Reproduction, Vancouver, BC, Canada, July 29-August 2, 2012.185 Cost effectiveness of maintaining research chicken populations
in situ or with cryopreserved semen or ovaries

- (Abstract Only)Silversides, F.G., Purdy, P.H., Blackburn, H.D. 2012. 185 Cost effectiveness of maintaining research chicken populations in situ or with cryopreserved semen or ovaries. Meeting Abstract. Poultry Science Association, Athens, GA, July 9-12.Comparative costs of programmes to conserve chicken genetic variation based on maintaining living populations or storing cryopreserved material

- (Peer Reviewed Journal)Silversides, F., Purdy, P.H., Blackburn, H.D. 2013. Comparative costs of programmes to conserve chicken genetic variation based on maintaining living populations or storing cryopreserved material. British Poultry Science. 53:599-607.Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity

- (Peer Reviewed Journal)Freking, B.A., Purdy, P.H., Spiller, S.F., Welsh, C.S., Blackburn, H.D. 2012. Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity. Journal of Animal Science. 90(8):2515-2523.Changing rooster sperm membranes to facilitate cryopreservation

- (Abstract Only)Graham, J.K., Travis, K.M., Purdy, P.H. 2012. Changing rooster sperm membranes to facilitate cryopreservation. Meeting Abstract. International Embryo Transfer Society, Phoenix, AZ. January 10, 2012.Cryoconservation of animal genetic resources. Animal Production and Health Guidelines No. 12

- (Book / Chapter)Blackburn, H.D., Godke, R., Purdy, P.H., Hiemstra, S., Woelders, H., Mariante, A., Pizzi, F., Boettcher, P. 2012. Cryoconservation of animal genetic resources. Animal Production and Health Guidelines No. 12. Complete Book. FAO, Rome p. 222.Genetic Structure and Diversity among U.S. sheep breeds: Identification of the major gene pools.

- (Peer Reviewed Journal)Blackburn, H.D., Paiva, S.R., Wildeus, S., Getz, W., Stobart, R., Bixby, D., Purdy, P.H., Welsh, C.S., Spiller, S.F., Brown, M.A. 2011. Genetic Structure and Diversity among U.S. sheep breeds: Identification of the major gene pools. Journal of Animal Science. 89:2336-2348.Sperm quality assessment in mammals by flow cytometry In: T.R. Tiersch and C.C. Green (eds.) Cryopreservation in Aquatic Species, 2nd Edition. World Aquaculture Society, Baton Rouge, LA.

- (Book / Chapter)Purdy, P.H. 2011. Sperm quality assessment in mammals by flow cytometry pp. 219-226. In: T.R. Tiersch and C.C. Green (eds.) Cryopreservation in Aquatic Species, 2nd Edition. World Aquaculture Society, Baton Rouge, LA.Effect of number of motile frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts

- (Peer Reviewed Journal)Spencer, K.W., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Stewart, T.S., Knox, R.V. 2010. Effect of number of motile frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts. Animal Reproduction Sciences. 121:259-266.Reproductive biotechnologies and management of animal genetic resources

- (Proceedings)Blackburn, H.D., Purdy, P.H. 2010. Reproductive biotechnologies and management of animal genetic resources. XIIIth European Poultry Conference, Tours France, August 23-27, 2010. Meeting Proceedings.Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics

- (Peer Reviewed Journal)Purdy, P.H., Tharp, N., Stewart, T., Spiller, S.F., Blackburn, H.D. 2010. Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics. Theriogenology. 74:1304-1310.Inseminating fresh or cryopreserved semen for maximum efficiency: implications for gene banks and industry

- (Peer Reviewed Journal)Blackburn, H.D., Silversides, F., Purdy, P.H. 2009. Inseminating fresh or cryopreserved semen for maximum efficiency: implications for gene banks and industry. Poultry Science. 88:2192-2198.Evaluation of Glycerol Removal Techniques, Cryoprotectants, and Insemination Methods for Cryopreserving Rooster Sperm with Implications for Breed and/or Line Regeneration

- (Peer Reviewed Journal)Purdy, P.H., Song, Y., Silversides, F.G., Blackburn, H.D. 2009. Evaluation of Glycerol Removal Techniques, Cryoprotectants, and Insemination Methods for Cryopreserving Rooster Sperm with Implications for Breed and/or Line Regeneration. Poultry Science. 88:2184-2191The fertility of ram sperm held for 24 hours at 5 oC prior to cryopreservation

- (Peer Reviewed Journal)Purdy, P.H., Moce, E., Stobart, R., Murdoch, W.J., Moss, G.E., Larson, B., Ramsey, S., Graham, J.K., Blackburn, H.D. 2009. The fertility of ram sperm held for 24 hours at 5 oC prior to cryopreservation. Animal Reproduction Sciences. 118:231-235.Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival

- (Peer Reviewed Journal)Moce, E., Purdy, P.H., Graham, J.K. 2009. Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Animal Reproduction Sciences. 118:236-247.Conservation of Swine Genetics by the National Animal Germplasm Program (NAGP) after One Decade

- (Popular Publication)Blackburn, H.D., Stewart, T., Purdy, P.H. 2009. Conservation of Swine Genetics by the National Animal Germplasm Program (NAGP) after One Decade. National Hog Farmer.Investigation of a novel non-surgical method of artificial insemination for sheep

- (Proceedings)PPurdy, P.H., Blackburn, H.D., Larson, B., Stobart, R. 2009. Investigation of a novel non-surgical method of artificial insemination for sheep. Western section of Amercan Society of animal Science, Fort Collins, CO, June 16-18, 2009.Influence of pre-cryopreservation pH and temperature on boar semen quality

- (Abstract Only)Tharp, N.E., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Stewart, T. 2009. Influence of pre-cryopreservation pH and temperature on boar semen quality. Midwest Section American Society of Animal Science, Des Moines, IA, March 16-18, 2008.Evaluation of new techniques to enhance the use of A.I. on farm.

- (Abstract Only)PPurdy, P.H., Stobart, R.H., Larson, B., Blackburn, H.D. 2009. Evaluation of new techniques to enhance the use of A.I. on farm. American Sheep Industry Association Annual Conference, San Diego, CA, January 21-24, 2009.Effect of number of motile sperm and number of inseminations using frozen boar semen on fertility in gilts

- (Trade Journal)Spencer, K., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Welsh, C.S., Stewart, T., Breen, S., Taibl, J., Yantis, B., Knox, R. 2008. Effect of number of motile sperm and number of inseminations using frozen boar semen on fertility in gilts. National Hog Farmer. http://nationalhogfarmer.com/genetics-reproduction/artificial-insemination/1215-boar-semen-use/.Perspectives on cryopreservation, quality control, and artificial insemination of frozen-thawed boar sperm in a national repository

- (Proceedings)Purdy, P.H., Knox, R., Singleton, W., Spencer, K., Spiller, S.F., Stewart, T., Tharp, N., Welsh, C.S., Blackburn, H.D. 2008. Perspectives on cryopreservation, quality control, and artificial insemination of frozen-thawed boar sperm in a national repository. Symposium Proceedings. Midwest Boar Stud Managers Conference, Saint Louis, MO August 7-8, 2008.Swine gene banking: A quality control perspective on collection, and analysis of samples for a national repository

- (Peer Reviewed Journal)Purdy, P.H. 2008. Gene banking: A quality control perspective on collection, and analysis of samples for a national repository. Theriogenology. 10.1016/j.theriogenology.2008.06.012Ubiquitination and its influence in boar sperm physiology and cryopreservation

- (Peer Reviewed Journal)Purdy, P.H. 2008. Ubiquitination and its influence in boar sperm physiology and cryopreservation. Theriogenology 70:818-826.Boar fertility and semen quality characteristics in lines of pigs selected for component traits of female reproduction

- (Abstract Only)Freking, B.A., Purdy, P.H., Welsh, C.S., Spiller, S.F., Blackburn, H.D. 2008. Boar fertility and semen quality characteristics in lines of pigs selected for component traits of female reproduction [abstract]. Journal of Animal Science Supplement. 86(E-Suppl. 2):208-209. Abstract #W50.Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology

- (Abstract Only)Purdy, P.H. 2008. Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology. American Society of Andrology Meeting, Albuquerque, New Mexico, April 2008. Pp. 49.Gene banking: A quality control perspective on collection, and analysis of samples for a national repository

- (Abstract Only)Purdy, P.H. 2007. Gene banking: A quality control perspective on collection, and analysis of samples for a national repository. Theriogenology. 6th International Conference on Boar Semen Preservation, Alliston, Ontario, Canada, August 11-15, 2007.Semen cryopreservation research seeks higher fertility rates in rainbow trout

- (Popular Publication)Silverstein, J.T., Purdy, P.H., Blackburn, H.D. 2007. Semen cryopreservation research seeks higher fertility rates in rainbow trout. Global Aquaculture Advocate. 10(2):84.Preserving Ram Semen: New Science Shows it can be Stored for up to Two Days Before Freezing

- (Popular Publication)Purdy, P.H. 2007. Preserving Ram Semen: New Science Shows it can be Stored for up to Two Days Before Freezing. Sheep! Magazine. p.40-41.Relationship of flow cytometric evaluations of cryopreserved rainbow trout Oncorhynchus mykiss sperm with fertility

- (Abstract Only)Purdy, P.H., Blackburn, H.D., Silverstein, J. 2007. Relationship of flow cytometric evaluations of cryopreserved rainbow trout Oncorhynchus mykiss sperm with fertility. Aquaculture 2007 Annual Meeting. p 734.A Method for Cryopreserving Chicken Primordial Germ Cells

- (Peer Reviewed Journal)Moore, D.T., Purdy, P.H., Blackburn, H.D. 2006. A Method for Cryopreserving Chicken Primordial Germ Cells. Poultry Science. 85:1784–1790.UBIQUITINATION OF BOAR SPERM: WHAT DOES THIS REVEAL ABOUT SPERM PHYSIOLOGY?

- (Abstract Only)Purdy, P.H. 2006. Ubiquitination of boar sperm: what does this reveal about sperm physiology? Annual Meeting of the Society for the Study of Reproduction, Omaha, NE, July 29-August 1, 2006. p.189.VARIATION IN SPERM CELL QUALITY IN CRYOPRESERVED BOAR SEMEN

- (Peer Reviewed Journal)Stewart, T., Gunsett, F., Welsh, C.S., Spiller, S.F., Purdy, P.H., Blackburn, H.D. 2006. Variation in sperm cell quality in cryopreserved boar semen. 8th World Congress of Genetics Applied in Livestock Production. 11(23):1-4.THE POST-THAW QUALITY OF RAM SPERM HELD FOR 0 TO 48 HOURS AT 5°C PRIOR TO CRYOPRESERVATION

- (Peer Reviewed Journal)Purdy, P.H. 2006. The post-thaw quality of ram sperm held for 0 to 48 hours at 5°c prior to cryopreservation. Animal Reproduction Sciences. 93:114-123.THE FLUIDITY OF CHINESE HAMSTER OVARY CELL AND BULL SPERM MEMBRANES AFTER CHOLESTEROL ADDITION

- (Peer Reviewed Journal)Purdy, P.H., Fox, M.H., Graham, J.K. 2005. The fluidity of chinese hamster ovary cell and bull sperm membranes after cholesterol addition. Cryobiology. 51:102-112.CHOLESTEROL-LOADED CYCLODEXTRIN IMPROVES POST-THAW GOAT SPERM MOTILITY

- (Abstract Only)Barrera-Compean, M.H., Purdy, P.H., Dzakuma, J.M., Newton, G.R., Nuti, L.C. 2005. Cholesterol-loaded cyclodextrin improves post-thaw goat sperm motility. Journal of Animal Science. 2005. 83 (Supl 1):153.A REVIEW ON GOAT SPERM CRYOPRESERVATION

- (Peer Reviewed Journal)Purdy, P.H. 2005. A review on goat sperm cryopreservation. Small Ruminant Research.A REVIEW ON GOAT SPERM CRYOPRESERVATION

- (Peer Reviewed Journal)Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Research. 63:215-225.EFFECTS OF HOLDING TIME PRIOR TO FREEZING ON THE MOTILITY, VIABILITY AND MEMBRANE BINDING ABILITY OF RAM SPERM

- (Abstract Only)Purdy, P.H. 2004. Effects of holding time prior to freezing on the motility, viability and membrane binding ability of ram sperm. 2004 Joint Annual Meeting of the American Dairy Science Association, American Society of Animal Science and Poultry Science Association, July 26-28, 2004, St. Louis, Missouri. Journal of Animal Science. 82(Suppl 1):367.EFFECT OF ADDING CHOLESTEROL TO BULL SPERM MEMBRANES ON SPERM CAPACITATION, THE ACROSOME REACTION, AND FERTILITY

- (Peer Reviewed Journal)Purdy, P.H., Graham, J.K. 2004. Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction, and fertility. Biology of Reproduction. 71:522-527.SPANISH BUCK SS-B INHIBIN/ACTIVIN (INHBB) MICROSATELLITE POLYMORPHISMS

- (Abstract Only)Realivasquez, R., Ericsson, S.A., Spiller, S.F., Campbell, W.T., Sternes, K.L., Purdy, P.H., Blackburn, H.D., Dzakuma, J.M. 2004. Spanish buck ss-b inhibin/activin (INHBB) microsatellite polymorphisms. American Society of Animal Science/American Society of Dairy Science/Poultry Science Association Annual Meeting, July 25-29, 2004, St. Louis, Missouri. Journal of Animal Science Supplement 2004. 82(Suppl 1): 377-378.INHA MICROSATELLITE POLYMORPHISMS IN ANGORA BUCKS

- (Abstract Only)Spiller, S.F., Ericsson, S.A., Realivasquez, R., Campbell, W.T., Sterns, K.L., Purdy, P.H., Blackburn, H.D., Dzakuma, J.M. 2004. INHA microsatellite polymorphisms in angora bucks. American Society of Animal Science/American Society of Dairy Science/Poultry Science Association Annual Meeting, July 25-29, 2004, St. Louis, Missouri. Journal of Animal Science 2004. 82(Suppl 1):378.EFFECTS OF THE FLAVONOIDS, SILIBININ AND CATECHIN, ON THE MOTILITY OF EXTENDED COOLED CAPRINE SPERM

- (Peer Reviewed Journal)Purdy, P.H., Ericsson, S.A., Dodson, R.E., Sternes, K.L., Garner, D.L. 2004. Effects of the flavonoids, silibinin and catechin, on the motility of extended cooled caprine sperm. Small Ruminant Research. 55:239-243.EFFECT OF CHOLESTEROL-LOADED CYCLODEXTRIN ON THE CRYOSURVIVAL OF BULL SPERM

- (Peer Reviewed Journal)Purdy, P.H., Graham, J.K. 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology. 48:36-45.CURRENT STATUS OF CRYOPRESERVING GOAT SEMEN

- (Abstract Only)Purdy, P.H. 2003. Current status of cryopreserving goat semen. Annual Meeting for the American Society of Animal Science, June 25-27, 2003, Phoenix, Arizona.
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