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                    U.S. DEPARTMENT OF AGRICULTURE
 Bioproducts Research: Albany, CA
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ARS Home Pacific West Area Albany, California Western Regional Research Center Bioproducts Research People & Locations







Charles  Lee
                        Bioproducts Research

                        




                        
                        Acting Research Leader
                        Phone: (510) 559-5858
                        
                        
                        Fax: (510) 559-5818(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsBioproducts and Biopolymers from Agricultural Feedstocks
                                                    In-House Appropriated
                                                                    (D)Accession Number: 438296Bioplastics and Natural Carotenoid from Cheese Byproducts
                                                    Reimbursable Cooperative Agreement
                                                                    (R)Accession Number: 443915Production of Environmentally Friendly PHA Biopolymers from Waste BioGas Derived from Under-Valued Biomass Feedstocks
                                                    Trust Fund Cooperative Agreement
                                                                    (T)Accession Number: 442420**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Compostable bioplastics from dairy processing coproducts

- (Abstract Only)Upcycling whey for production of compostable bioplastics

- (Abstract Only)Polyhydroxyalkanoate production by Priestia megaterium on glycerol

- (Abstract Only)Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids
                                                        

- (Peer Reviewed Journal)Cal, A.J., Sikkema, D., Hathwaik, U.I., Orts, W.J., Lee, C.C. 2023. Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids. PLOS. 18(7). https://doi.org/10.1371/journal.pone.0284377.Compression molded cellulose fiber foams
                                                        

- (Peer Reviewed Journal)Glenn, G.M., Orts, W.J., Klamczynski, A.P., Shogren, R., Hart-Cooper, W.M., Wood, D.F., Lee, C.C., Chiou, B. 2023. Compression molded cellulose fiber foams. Cellulose. 30:3489-3503. https://doi.org/10.1007/s10570-023-05111-0.Wounding and cold stress increase resin and rubber production of Parthenium argentatum cultivar G711
                                                        

- (Peer Reviewed Journal)Placido, D., McMahan, C.M., Lee, C.C. 2022. Wounding and cold stress increase resin and rubber production of Parthenium argentatum cultivar G711. Industrial Crops and Products. 193. Article 116174. https://doi.org/10.1016/j.indcrop.2022.116174.Potential of industrial hemp for phytoremediation of heavy metals
                                                        

- (Peer Reviewed Journal)Placido, D., Lee, C.C. 2022. Potential of industrial hemp for phytoremediation of heavy metals. Plants. 11(5). Article 595. https://doi.org/10.3390/plants11050595.Isolation of endophytes that suppress pathogen growth of chickpea (Cicer arietinum L.)
                                                        

- (Peer Reviewed Journal)Nadeem, A., O'Keeffe, T.L., Cal, A.J., Palumbo, J.D., Arshad, R., Bibi, N., Lee, C.C. 2022. Isolation of endophytes that suppress pathogen growth of chickpea (Cicer arietinum L.). Pakistan Journal of Botany. 54(5):1813-1820. https://doi.org/10.30848/pjb2022-5(22).Production of poly(3-hydroxybutyrate-co-4-hydroxybutryate) copolymers in Bacillus megaterium

- (Abstract Only)Production of polyhydroxyalkanoate copolymers containing 4-hydroxybutyrate in engineered Bacillus megaterium
                                                        

- (Peer Reviewed Journal)Cal, A.J., Kibblewhite, R.E., Sikkema, D.W., Torres, L.F., Hart-Cooper, W.M., Orts, W.J., Lee, C.C. 2020. Production of polyhydroxyalkanoate copolymers containing 4-hydroxybutyrate in engineered Bacillus megaterium. International Journal of Biological Macromolecules. 168:86-92. https://doi.org/10.1016/j.ijbiomac.2020.12.015.Biodegradation of cotton, PET, and PHB fibers in compost conditions

- (Peer Reviewed Journal)Lactic acid bacteria for growth promotion and fungal disease management in crops

- (Abstract Only)Nadeem, A., Bibi, N., Arshad, R., Lee, C.C. 2019. Lactic acid bacteria for growth promotion and fungal disease management in crops. Meeting Abstract. [abstract]. ASM Microbe 2019 conference, San Francisco, CA.  June 20-24, 2019.Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids

- (Abstract Only)Sikkema, D.W., Cal, A.J., Hathwaik, U.I., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2019. Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids. Meeting Abstract. [abstract]. ASM Microbe 2019 conference, San Francisco, CA.  June 20-24, 2019.Nucleation and plasticization with recycled low-molecular-weight poly-3-hydroxybutyrate toughens virgin poly-3-hydroxybutyrate
                                                        

- (Peer Reviewed Journal)Cal, A.J., Grubbs, B., Torres, L.F., Riiff, T.J., Orts, W.J., Lee, C.C. 2019. Nucleation and plasticization with recycled low-molecular-weight poly-3-hydroxybutyrate toughens virgin poly-3-hydroxybutyrate. Journal of Applied Polymer Science. 136(17):47432. https://doi.org/10.1002/app.47432.Polyhydroxyalkanoate production in pseudomonas

- (Abstract Only)Sikkema, D., Cal, A.J., Ponce, M.I., Orts, W.J., Lee, C.C., Hathwaik, U.I. 2018. Polyhydroxyalkanoate production in pseudomonas. Meeting Abstract. 25th Annual BEPS Conference. September 20-22, 2017. Albany, CA.Isolation of ligninolytic Bacillus sp. strains for depolymerization of alkali lignin
                                                        

- (Peer Reviewed Journal)Shah, T.A., Tabassum, R., Orts, W.J., Lee, C.C. 2019. Isolation of ligninolytic Bacillus sp. strains for depolymerization of alkali lignin. Journal of Environmental Progress and Sustainable Energy. 38(3):e13036. https://doi.org/10.1002/ep.13036.Biochemical characterization of caulobacter crescentus xylose dehydrogenase
                                                        

- (Peer Reviewed Journal)Lee, C.C., Jordan, D.B., Stoller, R.J., Kibblewhite, R.E., Wagschal, K.C. 2018. Biochemical characterization of caulobacter crescentus xylose dehydrogenase. International Journal of Biological Macromolecules. 118:1362-1367. https://doi.org/10.1016/j.ijbiomac.2018.06.124.Biochemical characterization of caulobacter crescentus xylose dehydrogenase
                                                        

- (Peer Reviewed Journal)Lee, C.C., Jordan, D.B., Stoller, J.R., Kibblewhite, R.E., Wagschal, K.C. 2018. Biochemical characterization of caulobacter crescentus xylose dehydrogenase. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.06.124.Absence or presence of metal ion activation in two structurally
similar GH43 beta-xylosidases
                                                        

- (Peer Reviewed Journal)Jordan, D.B., Stoller, J.R., Kibblewhite, R.E., Lee, C.C., Wagschal, K.C. 2018. Analysis of divalent-metal requirements of two GH43 ß-xylosidases by site-directed mutagenesis. Enzyme and Microbial Technology. 114:29-32. doi: 10.1016/j.enzmictec.2018.03.007.Production and uses of low molecular weight 3-hydroxybutyrate polymers and oligomers

- (Abstract Only)Cal, A.J., Grubbs, B., Torres, L.F., Riiff, T.J., Orts, W.J., Lee, C.C. 2017. Production and uses of low molecular weight 3-hydroxybutyrate polymers and oligomers. Meeting Abstract. 25th Annual BEPS Conference. September 20-22, 2017. Albany, CA.A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product
                                                        

- (Research Notes)Stoller, J.R., Wagschal, K.C., Lee, C.C., Jordan, D.B. 2017. A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product. Analytical Biochemistry. 523:46-49. doi: 10.1016/j.ab.2017.02.004.Conversion of biomass to value-added bioproducts by enzyme nanoassemblies

- (Abstract Only)Lee, C.C., Kibblewhite, R.E., Paavola, C.D., Wagschal, K.C. 2017. Conversion of biomass to value-added bioproducts by enzyme nanoassemblies. Meeting Abstract. American Chemical Society. p.100.Production and characterization of microbial polyhydroxyalkanoates

- (Abstract Only)Cal, A.J., Sikkema, D.W., Ponce, M.I., Orts, W.J., Lee, C.C. 2017. Production and characterization of microbial polyhydroxyalkanoates. Meeting Abstract. American Chemical Society. p.340.Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus
                                                        

- (Peer Reviewed Journal)Jordan, D.B., Stoller, J.R., Lee, C.C., Chan, V.J., Wagschal, K.C. 2016. Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus. Applied Biochemistry and Biotechnology. 182:250-260. doi: 10.1007/s12010-016-2324-0.Production of D-xylonic acid from hemicellulose using artificial enzyme complexes

- (Peer Reviewed Journal)Lee, C.C., Kibblewhite, R.E., Paavola, C., Orts, W.J., Wagschal, K.C. 2017. Production of D-xylonic acid from hemicellulose using artificial enzyme complexes. Journal of Microbiology and Biotechnology. 27(1):77–83 doi: 10.4014/jmb.1606.06041.Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth

- (Peer Reviewed Journal)Sharon, J.A., Hathwaik, L.T., Glenn, G.M., Imam, S.H., Lee, C.C. 2016. Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. Journal of Soil Science and Plant Nutrition. 16(2):525-536.Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications

- (Abstract Only)Lee, C.C., Wagschal, K.C., Paavola, C. 2016. Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications.[abstract]. USDA/NIFA Nanotechnology Meeting.Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus

- (Peer Reviewed Journal)Wagschal, K.C., Stoller, J.R., Chan, V.J., Lee, C.C., Grigorescu, A.A., Jordan, D.B. 2016. Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus. Molecular Biotechnology. 58(7):509-519. doi: 10.1007/s12033-016-9948-8.Production of glucaric acid from hemicellulose substrate by rosettasome enzyme assemblies

- (Peer Reviewed Journal)Lee, C.C., Kibblewhite, R.E., Paavola, C., Orts, W.J., Wagschal, K.C. 2016. Production of glucaric acid from hemicellulose substrate by rosettasome enzyme assemblies. Molecular Biotechnology. 58:489.Methanotrophic production of copolymer, poly(hydroxybutyrate-co-hydroxyvalerate), with high hydroxyvalerate content

- (Peer Reviewed Journal)Cal, A.J., Ponce, M.I., Franquivillanueva, D.M., Orts, W.J., Pieja, A., Lee, C.C. 2016. Methanotrophic production of copolymer, poly(hydroxybutyrate-co-hydroxyvalerate), with high hydroxyvalerate content. International Journal of Biological Macromolecules. 87:302.Isolation and divalent-metal activation of a ß-xylosidase, RUM630-BX

- (Peer Reviewed Journal)Jordan, D.B., Braker, J.D., Wagschal, K.C., Stoller, J.R., Lee, C.C. 2015. Isolation and divalent-metal activation of a ß-xylosidase, RUM630-BX. Enzyme and Microbial Technology. 82:158-163. doi: 10.1016/j.enzmictec.2015.10.001.Utilization of microbes to improve crop production

- (Proceedings)Sharon, J.A., Lee, C.C., Glenn, G.M. 2015. Utilization of microbes to improve crop production. UJNR meeting, Kanazawa, Ishikawa, Japan. November 14 - 19, 2015. Meeting Proceedings. 44:29-30.X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX
                                                        

- (Peer Reviewed Journal)Jordan, D.B., Braker, J.D., Wagschal, K., Lee, C.C., Chan, V.J., Dubrovska, I., Anderson, S., Wawrzak, Z. 2015. X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX. Applied Biochemistry and Biotechnology. 177:637-648. doi: 10.1007/s12010-015-1767-z.Enzyme nanoassemblies for biomass conversion

- (Abstract Only)Lee, C.C., Wagschal, K.C., Paavola, C. 2015. Enzyme nanoassemblies for biomass conversion. Meeting Abstract. Gordon Research Conference Abstracts, 1(10).Nanostructure enzyme assemblies for biomass conversion

- (Abstract Only)Lee, C.C., Kibblewhite, R.E., Templeton, P.D., Orts, W.J., Paavola, C., Wagschal, K.C. 2015. Nanostructure enzyme assemblies for biomass conversion. 37th Symposium for Biotechnology. Meeting Abstract. PSI: M110.Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans
                                                        

- (Peer Reviewed Journal)Wagschal, K.C., Jordan, D.B., Lee, C.C., Younger, A.R., Braker, J.D., Chan, V.J. 2014. Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans. Enzyme and Microbial Technology. 69:62-68.Lignocellulose hydrolysis by multienzyme complexes

- (Abstract Only)Templeton, P.D., Kibblewhite, R.E., Wagschal, K.C., Paavola, C., Lee, C.C. 2014. Lignocellulose hydrolysis by multienzyme complexes. American Chemical Society Abstracts. Biol (68).Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation
                                                        

- (Peer Reviewed Journal)Singh, S.K., Heng, C., Braker, J.D., Chan, V.J., Lee, C.C., Jordan, D.B., Yuan, L., Wagschal, K.C. 2013. Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation. Journal of Industrial Microbiology and Biotechnology. 41(3):489-498. doi: 10.1007/s10295-013-1377-0.Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease

- (Peer Reviewed Journal)Majeed, T., Tabassum, R., Orts, W.J., Lee, C.C. 2013. Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease. The Scientific World. doi: 10.1155/2013/396156.Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity

- (Peer Reviewed Journal)Jordan, D.B., Lee, C.C., Wagschal, K., Braker, J.D. 2013. Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity. Archives of Biochemistry and Biophysics. 533:79-87.Divalent metal activation of a GH43 ß-xylosidase

- (Peer Reviewed Journal)Lee, C.C., Braker, J.D., Grigorescu, A.A., Wagschal, K.C., Jordan, D.B. 2013. Divalent metal activation of a GH43 ß-xylosidase. Enzyme and Microbial Technology. 52(2):84-90.Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS

- (Peer Reviewed Journal)Gong, L., Xu, Q., Lee, C.C., Zhang, H. 2012. Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS. European Food Research and Technology. 235(1):169-176.Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture

- (Peer Reviewed Journal)Lee, C.C., Wagschal, K.C., Ruiping, L., Robertson, G.H., Kibblewhite, R.E., Orts, W.J. 2012. Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture. Journal of Industrial Microbiology and Biotechnology. 39:1245-1251. DOI:10.1007/s10295-012-1128-7.Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars

- (Abstract Only)Lee, C.C., Wagschal, K.C. 2013. Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars. [Abstract}. California American Chemical Society Section Meeting, April 25, 2012, Albany CA.Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications

- (Abstract Only)Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery

- (Peer Reviewed Journal)Wagschal, K.C., Lee, C.C. 2012. Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery. Analytical Biochemistry. 89: 83-85.Plant cell walls to ethanol.

- (Peer Reviewed Journal)Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library

- (Peer Reviewed Journal)Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Li, R., Orts, W.J. 2012. Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library. Protein Journal. 31(3):206-211.HILIC separation mechanisms of tetracyclines on amino bonded silica column

- (Peer Reviewed Journal)Li, R., Zhang, Y., Lee, C.C., Liu, L., Huang, Y. 2011. HILIC separation mechanisms of tetracyclines on amino bonded silica column. Journal of Separation Science. 34(13):1508-1516.A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector

- (Peer Reviewed Journal)Sasagawa, T., Moriya, S., Lee, C.C., Kitamoto, K., Arioka, M. 2011. A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector. Plasmid Journal. 65(1):65-69.Screening and characterization of hemicellulose-degrading enzymes

- (Abstract Only)Lee, C.C., Wagschal, K.C., Orts, W.J. Screening and characterization of hemicellulose-degrading enzymes. 2010 SIM Annual Meeting and Exhibition; Abstract S160b: p101.Screening assays for biomass-degrading enzymes

- (Peer Reviewed Journal)Lee, C.C. 2010. Screening assays for biomass-degrading enzymes. Biofuels. 1(4):575-588.Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures

- (Abstract Only)Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract.  85.Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water

- (Peer Reviewed Journal)Li, R., Zhang, Y., Lee, C.C., Lu, R., Huang, Y. Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water. Journal of Chromatography, 2010.  A 1217(11), pp 1799-1805.Cloning and characterization of alpha-glucuronidase enzyme

- (Abstract Only)Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.Cloning and characterization of alpha-glucuronidase enzyme

- (Abstract Only)Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion

- (Peer Reviewed Journal)Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.Cloning and characterization of an alpha-glucuronidase from a mixed microbial population

- (Peer Reviewed Journal)Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320.Molecular cloning and characterization of multidomain xylanase from manure library

- (Peer Reviewed Journal)Li, R., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2009. Molecular cloning and characterization of multidomain xylanase from manure library. World Journal of Microbiology and Biotechnology.  doi:10:1007/s11274-009-011106Engineer Sccharomyces cerevisiae for consolidated bioprocessing

- (Abstract Only)Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.Cloning biomass-degrading enzymes from environmental sources

- (Abstract Only)Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.Multimeric hemicellulases facilitate biomass conversion

- (Peer Reviewed Journal)Zhanmin, F., Wagschal, K.C., Wei, C., Montross, M., Lee, C.C., Yuan, L. 2009. Multimeric hemicellulases facilitate biomass conversion. Proceedings of the National Academy of Sciences. 75(6):1754-1757.An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening

- (Peer Reviewed Journal)Lee, C.C., Wagschal, K.C., Kibblewhite, R.E., Orts, W.J., Robertson, G.H., Wong, D. 2009. An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening. Applied Biochemistry and Biotechnology. 155(1-3):314-320.Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08

- (Peer Reviewed Journal)Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes

- (Peer Reviewed Journal)Fan, Z., Wagschal, K.C., Lee, C.C., Kong, Q., Shen, K.A., Maiti, I.B., Yuan, L. 2009. The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes. Biotechnology and Bioengineering. 102:684-692Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture

- (Peer Reviewed Journal)Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.Metagenomic gene discovery and enzyme engineering for biomass conversion

- (Abstract Only)Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.Gene discovery and engineering for biomass degradation

- (Proceedings)Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources.  2008. 50-51.Biofuels Group NSF DUSEL Project

- (Abstract Only)Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme

- (Peer Reviewed Journal)Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology.  57:301-305.An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening

- (Abstract Only)Lee, C.C., Wagschal, K.C., Accinelli, R., Orts, W.J., Robertson, G.H., Wong, D. 2008. An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening. Biotechnology for Fuels and Chemicals Symposium Proceedings. Abstract 6-26.Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125

- (Peer Reviewed Journal)Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.Cloning and characterization of xylanase gene from hot spring microorganism

- (Abstract Only)Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.Developing enzyme systems for biomass deconstruction

- (Abstract Only)Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217SYNERGISTIC ACTION OF ALPHA-AMYLASE AND GLUCOAMYLASE ON THE HYDROLYSIS OF NATIVE STARCH GRANULES

- (Peer Reviewed Journal)Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C., Wagschal, K.C. 2007. Synergistic action of alpha-amylase and glucoamylase on the hydrolysis of native starch granules. Protein and Peptide Letters.  26:159-164.SATB1 Packages Densely Looped, Transcriptionally Active Chromatin for Coordinated Expression of Cytokine Genes

- (Peer Reviewed Journal)Cai, S., Lee, C.C., Kohwi-Shigematsu, T. 2006. Satb1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. Nature Genetics. (38):1278-1288EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE

- (Abstract Only)Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae.  Division of Biological Chemistry, Enzymes Program.  Abstract 220.GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE

- (Peer Reviewed Journal)Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES

- (Abstract Only)Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract).  P8.SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS

- (Abstract Only)Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005.  American Society of Biochemistry & Molecular Biology, San Francisco, CA.  April 1-4, 2006, Program 73.16, vol A27.CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES

- (Abstract Only)Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals.  Paper No. 1A-41.CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME

- (Peer Reviewed Journal)Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme.  Extremophiles. 10:295-300.NATIVE OR RAW STARCH DIGESTION

- (Review Article)Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion:  A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.ISOLATION AND CHARACTERIZATION OF A PSYCHROPHILIC XYLANASE ENZYME FROM FLAVOBACTERIUM SP.

- (Peer Reviewed Journal)Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.CHARACTERIZATION OF ACTIVE LENTINULA EDODES GLUCOAMYLASE EXPRESSED AND SECRETED BY SACCHAROMYCES CEREVISIAE

- (Peer Reviewed Journal)Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal.  vol 24, pp 455-463.CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES

- (Abstract Only)Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S.  2005.  Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO.  Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.ENZYME-COUPLED ASSAY FOR BETA-XYLOSIDASE HYDROLYSIS OF NATURAL SUBSTRATES

- (Peer Reviewed Journal)Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology.  71:5318-5323.EVOLVED ENZYMES FOR COLD OR NON-COOKING HYDROLYSIS OF STARCHES TO FERMENTATION FEEDSTOCK

- (Abstract Only)Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005.  Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV.  2005 CDROM.EXPRESSION AND CHARACTERIZATION OF ACTIVE LENTINUS EDODES GLUCOAMYLASE IN SACCHAROMYCES CEREVISIAE

- (Abstract Only)Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005.  Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05.  Paper No 11277.CLONING AND CHARACTERIZATION OF BIOMASS DEGRADING ENZYMES

- (Abstract Only)Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005.  Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO.  Paper No. IB-41 (Abstract).DIRECTED EVOLUTION OF BARLEY ALPHA-AMYLASE FOR INCREASED ACTIVITY

- (Abstract Only)Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H.  2004.  Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA.  Paper No. 144 (Abstracts).HETEROGENEOUS BIOCATALYIC LIQUEFACTION AND SACCHARIFICAATION OF STARCH FOR ETHANOL

- (Abstract Only)Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.IN VITRO EVOLUTION OF HIGH-ACTIVITY BARLEY ALPHA-AMYLASE FOR RAW STARCH DIGESTION

- (Abstract Only)Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California.  Abstract.HIGH-ACTIVITY BARLEY ALPHA-AMYLASE BY DIRECTED EVOLUTION

- (Peer Reviewed Journal)Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004.  High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.RAPID SELECTION OF ALPHA-AMYLASE MUTANTS IN YEAST LIBRARIES FOR RAW STARCH ACTIVITY

- (Abstract Only)Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity.  2003.  Society of Biomolecular Screening Annual Conference, Portland, Oregon.ENGINEERING APHA-AMYLASE FOR ENHANCED ACTIVITY BY MOLECULAR EVOLUTION

- (Abstract Only)Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution.  American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts.   Abstract No. B38.AN EPISOMAL EXPRESSION VECTOR FOR SCREENING MUTANT GENE LIBRARIES IN PICHIA PASTORIS

- (Peer Reviewed Journal)Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004.  An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.DIRECT SCREENING OF LIBRARIES OF YEAST CLONES FOR ALPHA-AMYLASE ACTIVITY ON RAW STARCH

- (Peer Reviewed Journal)Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.CLONING AND CHARACTERIZATION OF THE XYN11A GENE FROM LENTINULA EDODES

- (Peer Reviewed Journal)Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.
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