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ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Bioenergy Research People







Ronald E Hector

(Ron)    Research Molecular Biologist Biography and Research **Ronald E. Hector – Research Molecular Biologist** Ron Hector has worked as molecular biologist at ARS in Peoria, IL since 2006. After receiving his B.S. in Chemical Engineering from Ohio University and Ph.D. in Molecular Genetics and Microbiology from the University of Florida, he conducted postgraduate research on molecular mechanisms of DNA damage sensing and repair, and telomere length regulation at the Cleveland Clinic Lerner Research Institute.  Currently, Dr. Hector is a member of a team of scientists that conducts research on the development of microorganisms and fermentation processes for the production of fuels and chemicals from agricultural materials (e.g., non-food crops and agricultural residues). The goals of his current research focus on metabolic engineering of the industrial yeast *Saccharomyces cerevisiae* (also known as Brewer’s yeast) to increase conversion of biomass-derived sugars to fuel alcohols and higher-value chemicals which are typically produced from oil. He uses metabolic engineering, molecular genetic, and comparative genomics and transcriptomics methods to address these objectives. ProjectsTechnologies to Improve Conversion of Biomass-Derived Sugars to BioproductsIn-House Appropriated (D)Accession Number:438817
 **Publications Indexed by Scopus** Click here to view Scopus entries for **Ronald Hector**in a new browser window. (The Scopus results may not reflect a complete or accurate listing for this scientist. Some journals may not be indexed by Scopus, and there may be articles by other authors of the same name listed by this search). Click here to view publications indexed by Google Scholar for **Ronald Hector**in a new browser window
 Publications(Clicking on the reprint iconReprint Icon  will take you to the publication reprint.)Novel technologies for butyric acid fermentation: use of cellulosic biomass, rapid bioreactor, and efficient product recovery-(Peer Reviewed Journal) Qureshi, N., Ashby, R.D., Nichols, N.N., Hector, R.E. 2024. Novel technologies for butyric acid fermentation: use of cellulosic biomass, rapid bioreactor, and efficient product recovery. Fermentation. https://doi.org/10.3390/fermentation10030142.Metabolic engineering of a stable haploid strain derived from lignocellulosic inhibitor tolerant Saccharomyces cerevisiae natural isolate YB-2625-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A., Nichols, N.N. 2023. Metabolic engineering of a stable haploid strain derived from lignocellulosic inhibitor tolerant Saccharomyces cerevisiae natural isolate YB-2625. Biotechnology for Biofuels and Bioproducts. https://doi.org/10.1186/s13068-023-02442-9.Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass-(Peer Reviewed Journal) Dias Lopes, D., Dien, B.S., Hector, R.E., Singh, V., Thompson, S.R., Slininger, P.J., Boundy-Mills, K., Sujit, J.S., Rao, C.V. 2023. Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass. Journal of Industrial Microbiology and Biotechnology. https://doi.org/10.1093/jimb/kuad040.Microcystin-detoxifying recombinant Saccharomyces cerevisiae expressing the mlrA gene from Sphingosinicella microcystinivorans B9-(Peer Reviewed Journal) de Godoi Silva, F., Dias Lopes, D., Hector, R.E., do Nascimento, M., de Avila Miguel, T., Kuroda, E., Andrade de Nobreag, G., Harada, K., Hirooka, E. 2023. Microcystin-detoxifying recombinant Saccharomyces cerevisiae expressing the mlrA gene from Sphingosinicella microcystinivorans B9. Microorganisms. 11(3). Article 575. https://doi.org/10.3390/microorganisms11030575.Growth of Coniochaeta species on acetate in biomass sugars-(Peer Reviewed Journal) Nichols, N.N., Mertens, J.A., Frazer, S.E., Hector, R.E. 2022. Growth of Coniochaeta species on acetate in biomass sugars. Fermentation. 8(12). Article 721. https://doi.org/10.3390/fermentation8120721.Identification of mutations responsible for improved xylose utilization in an adapted xylose isomerase expressing Saccharomyces cerevisiae strain-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A., Nichols, N.N. 2022. Identification of mutations responsible for improved xylose utilization in an adapted xylose isomerase expressing Saccharomyces cerevisiae strain. Fermentation. 8(12). Article 669. https://doi.org/10.3390/fermentation8120669.Increased expression of the fluorescent reporter protein ymNeonGreen in Saccharomyces cerevisiae by reducing RNA secondary structure near the start codon-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A., Nichols, N.N. 2021. Increased expression of the fluorescent reporter protein ymNeonGreen in Saccharomyces cerevisiae by reducing RNA secondary structure near the start codon. Biotechnology Reports. 33: Article e00697. https://doi.org/10.1016/j.btre.2021.e00697.Performance of xylose-fermenting yeasts in oat and soybean hulls hydrolysate and improvement of ethanol production using immobilized cell systems-(Peer Reviewed Journal) Cortivo, P.R.D, Aydos, L.F., Hickert, L.R., Rosa, C.A., Hector, R.E., Mertens, J.A., Ayub, M.A.Z. 2021. Performance of xylose-fermenting yeasts in oat and soybean hulls hydrolysate and improvement of ethanol production using immobilized cell systems. Biotechnology Letters. 43:2011-2026. https://doi.org/10.1007/s10529-021-03182-2.Abatement of inhibitors in recycled process water from biomass fermentations relieves inhibition of a Saccharomyces cerevisiae penthose phosphate pathway mutant-(Peer Reviewed Journal) Nichols, N.N., Hector, R.E., Mertens, J.A., Frazer, S.E. 2020. Abatement of inhibitors in recycled process water from biomass fermentations relieves inhibition of a Saccharomyces cerevisiae penthose phosphate pathway mutant. Fermentation. 6(4). Article 107. https://doi.org/10.3390/fermentation6040107.Impact of stress-response related transcription factor overexpression on lignocellulosic inhibitor tolerance of Saccharomyces cerevisiae environmental isolates-(Peer Reviewed Journal) Mertens, J.A., Skory, C.D., Nichols, N.N., Hector, R.E. 2020. Impact of stress-response related transcription factor overexpression on lignocellulosic inhibitor tolerance of Saccharomyces cerevisiae environmental isolates. Biotechnology Progress. 37(2). Article e3094. https://doi.org/10.1002/btpr.3094.Development and characterization of Saccharomyces cerevisiae strains genetically modified to over-express the pentose phosphate pathway regulating transcription factor STB5 in the presence of xylose-(Peer Reviewed Journal) Hohenschuh, W., Hector, R.E., Mertens, J.A., Murthy, G.S. 2020. Development and characterization of Saccharomyces cerevisiae strains genetically modified to over-express the pentose phosphate pathway regulating transcription factor STB5 in the presence of xylose. Systems Microbiology and Biomanufacturing. 1: 42-57. https://doi.org/10.1007/s43393-020-00002-y.Using high throughput data and dynamic flux balance modeling techniques to identify points of constraint in xylose utilization in Saccharomyces cerevisiae-(Peer Reviewed Journal) Hohenschuh, W., Hector, R.E., Chaplen, F., Murthy, G.S. 2021. Using high throughput data and dynamic flux balance modeling techniques to identify points of constraint in xylose utilization in Saccharomyces cerevisiae. Systems Microbiology and Biomanufacturing. 1: 58-75. https://doi.org/10.1007/s43393-020-00003-x.Defining the eco-enzymological role of the fungal strain Coniochaeta sp. 2T2.1 in a tripartite lignocellulolytic microbial consortium-(Peer Reviewed Journal) Jimenez, D., Wang, Y., de Mares, M., Cortes-Tolalpa, L., Mertens, J.A., Hector, R.E., Lin, J., Johnson, J., Lipzen, A., Barry, K., Mondo, S.J., Grigoriev, I.V., Nichols, N.N., Van Elsas, J.D. 2019. Defining the eco-enzymological role of the fungal strain Coniochaeta sp. 2T2.1 in a tripartite lignocellulolytic microbial consortium. FEMS Microbiology Ecology. 96(1). Article fiz186. https://doi.org/10.1093/femsec/fiz186.Recycle of fermentation process water through mitigation of inhibitors in dilute-acid corn stover hydrolysate-(Peer Reviewed Journal) Nichols, N.N., Mertens, J.A., Dien, B.S., Hector, R.E., Frazer, S.E. 2019. Recycle of fermentation process water through mitigation of inhibitors in dilute-acid corn stover hydrolysate. Bioresource Technology. 9:100349. https://doi.org/10.1016/j.biteb.2019.100349.Genome expansion by allopolyploidization in the fungal strain Coniochaeta 2T2.1 and its exceptional lignocellulolytic machinery-(Peer Reviewed Journal) Mondo, S.J., Jimenez, D.J., Hector, R.E., Lipzen, A., Yan, M., Labutti, K., Barry, K., Dirk Van Elsas, J., Grigoriev, I.V., Nichols, N.N. 2019. Genome expansion by allopolyploidization in the fungal strain Coniochaeta 2T2.1 and its exceptional lignocellulolytic machinery. Biotechnology for Biofuels. 12:229. https://doi.org/10.1186/s13068-019-1569-6.Development and characterization of vectors for tunable expression of both xylose-regulated and constitutive gene expression in Saccharomyces yeasts-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A., Nichols, N.N. 2019. Development and characterization of vectors for tunable expression of both xylose-regulated and constitutive gene expression in Saccharomyces yeasts. New Biotechnology. 53:16-23. https://doi.org/10.1016/j.nbt.2019.06.006.Factors affecting production of xylitol by the furfural-metabolizing fungus Coniochaeta ligniaria-(Peer Reviewed Journal) Nichols, N.N., Hector, R.E., Frazer, S.E. 2019. Factors affecting production of xylitol by the furfural-metabolizing fungus Coniochaeta ligniaria. Current Trends in Microbiology. 12: 109-119.Genetic transformation of Coniochaeta sp. 2T2.1, key fungal member of a lignocellulose-degrading microbial consortium-(Peer Reviewed Journal) Nichols, N.N., Hector, R.E., Frazer, S.E. 2019. Genetic transformation of Coniochaeta sp. 2T2.1, key fungal member of a lignocellulose-degrading microbial consortium. Biology Methods and Protocols. 4:1-5. https://doi.org/10.1093/biomethods/bpz001.Engineering Candida phangngensis – an oleaginous yeast from the Yarrowia clade – for enhanced detoxification of lignocellulose-derived inhibitors and lipid overproduction-(Peer Reviewed Journal) Quarterman, J.C., Slininger, P.J., Hector, R.E., Dien, B.S. 2018. Engineering Candida phangngensis – an oleaginous yeast from the Yarrowia clade – for enhanced detoxification of lignocellulose-derived inhibitors and lipid overproduction. Federation Of European Microbiological Societies Yeast Research. 18(8):foy102. https://doi.org/10.1093/femsyr/foy102.Screening for inhibitor tolerant Saccharomyces cerevisiae strains from diverse environments for use as platform strains for production of fuels and chemicals from biomass-(Peer Reviewed Journal) Mertens, J.A., Kelly, A., Hector, R.E. 2018. Screening for inhibitor tolerant Saccharomyces cerevisiae strains from diverse environments for use as platform strains for production of fuels and chemicals from biomass. Bioresource Technology. 3:154-161. https://doi.org/10.1016/j.biteb.2018.07.006.Association of improved oxidative stress tolerance and alleviation of glucose repression with superior xylose-utilization capability by a natural isolate of Saccharomyces cerevisiae-(Peer Reviewed Journal) Cheng, C., Tang, R., Xiong, L., Hector, R.E., Bai, F., Zhao, X. 2018. Association of improved oxidative stress tolerance and alleviation of glucose repression with superior xylose-utilization capability by a natural isolate of Saccharomyces cerevisiae. Biotechnology for Biofuels. 11:28. https://doi.org/10.1186/s13068-018-1018-y.Fermentation of oat and soybean hull hydrolysates into ethanol and xylitol by recombinant industrial strains of Saccharomyces cerevisiae under diverse oxygen environments-(Peer Reviewed Journal) Cortivo, P.R.D., Hickert, L.R.H, Hector, R., Ayub, M.A.Z. 2018. Fermentation of oat and soybean hull hydrolysates into ethanol and xylitol by recombinant industrial strains of Saccharomyces cerevisiae under diverse oxygen environments. Industrial Crops and Products. 113:10-18. doi: 10.1016/j.indcrop.2018.01.010.Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates-(Peer Reviewed Journal) Dias-Lopes, D., Rosa, C.A, Hector, R.E., Dien, B.S., Mertens, J.A., Ayub, M.A.Z. 2017. Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates. Journal of Industrial Microbiology and Biotechnology. 44(11):1575-1588. doi: 10.1007/s10295-017-1979-z.Draft genome sequence of the D-Xylose-Fermenting yeast Spathaspora xylofermentans UFMG-HMD23.3-(Peer Reviewed Journal) Lopes, D.D., Cibulski, S.P., Mayer, F.Q., Siqueira, F.M., Rosa, C.A., Hector, R.E., Ayub, M.A.Z. 2017. Draft genome sequence of the D-Xylose-Fermenting yeast Spathaspora xylofermentans UFMG-HMD23.3. Genome Announcements. 5(33):e00815-17.The impact of stress-response related transcription factors on lignocellulosic hydrolysate inhibitor tolerance of Saccharomyces strains-(Abstract Only) Mertens, J.A., Hector, R.E., Skory, C.D. 2017. The impact of stress-response related transcription factors on lignocellulosic hydrolysate inhibitor tolerance of Saccharomyces strains [abstract]. Meeting Abstract for Society for Industrial Microbiology, Denver, Colorado, 07/30-08/3/2017. poster #11.A synthetic hybrid promoter for xylose-regulated control of gene expression in Saccharomyces yeasts-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A. 2017. A synthetic hybrid promoter for xylose-regulated control of gene expression in Saccharomyces yeasts. Molecular Biotechnology. 59(1):24-33. doi: 10.1007/s12033-016-9991-5.Draft genome sequence of Coniochaeta ligniaria NRRL 30616, a lignocellulolytic fungus for bioabatement of inhibitors in plant biomass hydrolysates-(Peer Reviewed Journal) Jimenez, D.J., Hector, R.E., Riley, R., Lipzen, A., Kuo, R.C., Amirebrahimi, M., Barry, K.W., Grigoriev, I.V., Dirk van Elsas, J., Nichols, N.N. 2017. Draft genome sequence of Coniochaeta ligniaria NRRL 30616, a lignocellulolytic fungus for bioabatement of inhibitors in plant biomass hydrolysates. Genome Announcements. 5(4):e01476-16. doi: 10.1128/genomeA.01476-16.Modification of the mannitol biosynthetic pathway in Aureobasidium pullulans to alter the structure of the polyol lipid liamocin-(Abstract Only) Skory, C.D., Price, N.P.J., Leathers, T.D., Manitchotpisit, P., Hector, R.E., Bowman, M.J., Rich, J.O. 2016. Modification of the mannitol biosynthetic pathway in Aureobasidium pullulans to alter the structure of the polyol lipid liamocin [abstract]. Society for Industrial Microbiology and Biotechnology. Poster P39, Paper 32487.A dynamic flux balance model and bottleneck identification of glucose, xylose, xylulose co-fermentation in Saccharomyces cerevisiae-(Peer Reviewed Journal) Hohenschuh, W., Hector, R.E., Murthy, G.S. 2015. A dynamic flux balance model and bottleneck identification of glucose, xylose, xylulose co-fermentation in Saccharomyces cerevisiae. Bioresource Technology. 188:153-160.Triacetic acid lactone production in industrial Saccharomyces yeast strains-(Peer Reviewed Journal) Saunders, L.P., Bowman, M.J., Mertens, J.A., Da Silva, N.A., Hector, R.E. 2015. Triacetic acid lactone production in industrial Saccharomyces yeast strains. Journal of Industrial Microbiology and Biotechnology. 42:711-721.Creation of a synthetic xylose-inducible promoter for Saccharomyces cerevisiae-(Abstract Only) Hector, R.E., Card, K.A. 2014. Creation of a synthetic xylose-inducible promoter for Saccharomyces cerevisiae [abstract].Triacetic acid lactone production from Saccharomyces cerevisiae-(Abstract Only) Saunders, L.P., Bowman, M.J., Hector, R.E. 2014. Triacetic acid lactone production from Saccharomyces cerevisiae [abstract].Comparisons of five Saccharomyces cerevisiae strains for ethanol production from SPORL pretreated lodgepole pine-(Peer Reviewed Journal) Zhou, H., Lan, T., Dien, B.S., Hector, R.E., Zhu, J.Y. 2014. Comparisons of five Saccharomyces cerevisiae strains for ethanol production from SPORL pretreated lodgepole pine. Biotechnology Progress. 30(5):1076-1083.Biological abatement of inhibitors in rice hull hydrolyzate and fermentation to ethanol using conventional and engineered microbes-(Peer Reviewed Journal) Nichols, N.N., Hector, R.E., Saha, B.C., Frazer, S.E., Kennedy, G.J. 2014. Biological abatement of inhibitors in rice hull hydrolyzate and fermentation to ethanol using conventional and engineered microbes. Biomass and Bioenergy. 67:79-88.Bioconversion of beetle-killed lodgepole pine using SPORL: Process scale-up design, lignin co-product, and high solids fermentation without detoxification-(Peer Reviewed Journal) Zhou, H., Zhu, J.Y., Luo, X., Leu, S.-Y., Wu, X., Gleisner, R., Dien, B.S., Hector, R.E., Yang, D., Qiu, X., Horn, E., Negron, J. 2013. Bioconversion of beetle-killed lodgepole pine using SPORL: Process scale-up design, lignin co-product, and high solids fermentation without detoxification. Industrial and Engineering Chemistry Research. 52:16057-16065.Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24-(Peer Reviewed Journal) Hector, R.E., Dien, B.S., Cotta, M.A., Mertens, J.A. 2013. Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24. Biotechnology for Biofuels. 6:84.Conversion of switchgrass to ethanol using dilute ammonium hydroxide pretreatment: influence of ecotype and harvest maturity-(Peer Reviewed Journal) Dien, B.S., O'Bryan, P.J., Hector, R.E., Iten, L.B., Mitchell, R.B., Qureshi, N., Sarath, G., Vogel, K.P., Cotta, M.A. 2013. Conversion of switchgrass to ethanol using dilute ammonium hydroxide pretreatment: influence of ecotype and harvest maturity. Environmental Technology. 34(13-14):1837-1848.Simultaneous detoxification, saccharification, and ethanol fermentation of weak-acid hydrolyzates-(Peer Reviewed Journal) Klasson, K.T., Dien, B.S., Hector, R.E. 2013. Simultaneous detoxification, saccharification, and ethanol fermentation of weak-acid hydrolyzates. Industrial Crops and Products. 49:292-298.Reduction of fermentation lag phase in biofuel production using a novel activated biochar material-(Proceedings) Klasson, K.T., Dien, B.S., Hector, R.E. 2012. Reduction of fermentation lag phase in biofuel production using a novel activated biochar material. In: Proceedings of the U.S.-Japan Cooperative Program in Natural Resources Food and Agriculture Panel, USDA-ARS, December 8-13, 2012, Wyndmoor, Pennsylvania.  pp. 1-2.High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation-(Peer Reviewed Journal) Lan, T.Q., Gleisner, R., Zhu, J.Y., Dien, B.S., Hector, R.E. 2013. High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation. Bioresource Technology. 127:291-297.Expression of novel bacterial xylose isomerases in Saccharomyces cerevisiae-(Abstract Only) Hector, R.E., Dien, B.S., Cotta, M.A., Hughes, S.R. 2012. Expression of novel bacterial xylose isomerases in Saccharomyces cerevisiae [abstract]. Society for Industrial Microbiology & Biotechnology Annual Meeting, 8/12-8/16/12, Washington, DC.Genetically engineered Escherichia coli FBR5:  Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose-(Peer Reviewed Journal) Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Hector, R.E., Cotta, M.A., Hughes, S.R. 2012. Genetically engineered Escherichia coli FBR5:  Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose. Biotechnology Progress. 28(5):1167-1178.Genetically engineered Escherichia coli FBR5:  Part II. Ethanol production from xylose and simultaneous product recovery-(Peer Reviewed Journal) Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Cotta, M.A., Hughes, S.R., Hector, R.E. 2012. Genetically engineered Escherichia coli FBR5:  Part II. Ethanol production from xylose and simultaneous product recovery. Biotechnology Progress. 28(5):1179-1185.Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation-(Peer Reviewed Journal) Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2012. Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation. Bioresource Technology. 110:437-447.Plant cell walls to ethanol.-(Peer Reviewed Journal) Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.Biochemical processing of reed canarygrass into fuel ethanol-(Peer Reviewed Journal) Dien, B.S., Casler, M.D., Hector, R.E., Iten, L.B., Nichols, N.N., Mertens, J.A., Cotta, M.A. 2012. Biochemical processing of reed canarygrass into fuel ethanol. International Journal of Low-Carbon Technologies. 7:338-347.Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry-(Peer Reviewed Journal) Mertens, J.A., Hector, R.E., Bowman, M.J. 2012. Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry. Thermochimica Acta. 527:219-222.Effect of cellulosic sugar degradation products (furfural and hydroxymethylfurfural) on acetone-butanol-ethanol (ABE) fermentation using Clostridium beijerinckii P260-(Peer Reviewed Journal) Qureshi, N., Bowman, M.J., Saha, B.C., Hector, R.E., Berhow, M.A., Cotta, M.A. 2012. Effect of cellulosic sugar degradation products (furfural and hydroxymethylfurfural) on acetone-butanol-ethanol (ABE) fermentation using Clostridium beijerinckii P260. Journal of Food and Bioproducts Processing. 90:533-540.Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation-(Peer Reviewed Journal) Hector, R.E., Mertens, J.A., Bowman, M.J., Nichols, N.N., Cotta, M.A., Hughes, S.R. 2011. Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation. Yeast. 28:645-660.Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol-(Abstract Only) Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol [abstract]. American Society for Mass Spectrometry. p. 66.Butanol productivity enhancers in wheat straw hydrolyzate: employing potential of enhanced reaction rate-(Abstract Only) Qureshi, N., Bowman, M.J., Hector, R.E., Saha, B.C., Cotta, M.A. 2011. Butanol productivity enhancers in wheat straw hydrolyzate: employing potential of enhanced reaction rate [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper No. 11-11.Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain-(Abstract Only) Dien, B.S., Hector, R.E., Casler, M.D., Cotta, M.A. 2011. Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper No. 4-18.Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry-(Abstract Only) Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper NO. 10-19.Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition-(Peer Reviewed Journal) Dien, B.S., Miller, D.J., Hector, R.E., Dixon, R.A., Chen, F., McCaslin, M., Risen, P., Sarath, G., Cotta, M.A. 2011. Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition. Bioresource Technology. 102(11):6479-6486.Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion-(Peer Reviewed Journal) Hector, R.E., Dien, B.S., Cotta, M.A., Qureshi, N. 2011. Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion. Journal of Industrial Microbiology and Biotechnology. 38(9):1193-1202.Biochemical Conversion of Reduced Lignin Alfalfa Stems Into Ethanol-(Abstract Only)Cloning and Expression of Novel Bacterial Xylose Isomerases in Saccharomyces cerevisiae-(Abstract Only) Hector, R.E., Dien, B.S., Cotta, M.A., Hughes, S.R. 2010. Cloning and expression of novel bacterial xylose isomerases in Saccharomyces cerevisiae. Biotechnology for Fuels and Chemicals Symposium. Abstract #1.Production of Butanol (A Biofuel) from Agricultural Residues:  Part I - Use of Barley Straw Hydrolysate-(Peer Reviewed Journal) Qureshi, N., Saha, B.C., Dien, B., Hector, R.E., Cotta, M.A. 2010. Production of Butanol (a Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate. Biomass and Bioenergy. 34(4):559-565.Production of Butanol (a Biofuel) from Agricultural Residues:  Part II - Use of Corn Stover and Switchgrass Hydrolysates-(Peer Reviewed Journal) Qureshi, N., Saha, B.C., Hector, R.E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M.J., Sarath, G., Cotta, M.A. 2010. Production of butanol (a Biofuel) from agricultural residues: Part II - Use of corn stover and switchgrass hydrolysates. Biomass and Bioenergy. 34(4):566-571.Enhanced Production of Carboxylic Acids by Engineering of Rhizopus-(Abstract Only) Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Enhanced Production of Carboxylic Acids by Engineering of Rhizopus [abstract].Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus-(Abstract Only) Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus [abstract]. United States-Japan Natural Resources Food and Agriculture Panel. p. 160-163.Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus-(Proceedings) Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus. United States-Japan Cooperative Program in Natural Resources. p. 160-163.Genetic Engineering of Rhizopus for Enhancing Lactic Acid Production-(Abstract Only) Rich, J.O., Skory, C.D. 2009. Genetic Engineering of Rhizopus for enhancing lactic acid production [abstract]. Conference on Fermentation Technology for Value Added Agricultural Products. Paper Fer406. p. 25.Report membrane transport of lactic acid in the filamentous fungus Rhizopus-(Peer Reviewed Journal) Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Report membrane transport of lactic acid in the filamentous fungus Rhizopus.  KKU Research Journal. 15(9):826-831.Analysis of a Functional Lactate Permease in the Fungus Rhizopus-(Peer Reviewed Journal) Skory, C.D., Hector, R.E., Gorsich, S., Rich, J.O. 2010. Analysis of a functional lactate permease in the fungus Rhizopus. Enzyme and Microbial Technology. 46(1):43-50.The Saccharomyces cerevisiae YMR315W Gene Encodes an NADP(H)-Specific Oxidoreductase Regulated by the Transcription Factor Stb5p in Response to NADPH Limitation-(Peer Reviewed Journal) Hector, R.E., Bowman, M.J., Skory, C.D., Cotta, M.A. 2009. The Saccharomyces cerevisiae YMR315W gene encodes an NADP(H)-specific Oxidoreductase regulated by the transcription factor Stb5p in response to NADPH limitation. New Biotechnology. 26(3/4):171-180.Evaluation of engineered xylose-fermenting industrial strains of Saccharomyces cerevisiae for improved ethanol production from lignocellulosic feedstocks-(Abstract Only)Conversion of switchgrass to sugars and ethanol using dilute ammonium hydroxide pretreatment-(Abstract Only)GMAX Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically-(Abstract Only) Hughes, S.R., Tasaki, K., Moser, B., Doll, K., Butt, T., Sterner, D., Bischoff, K., Hector, R., Jones, M., and Bang, S. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit. Abstract No. 2. p. 3.Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates-(Abstract Only) Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated high throughput GMAX-L strains of Saccharomyces cereviciae for profitable cellulosic ethanol production from industrial hydrosylates [abstract]. Society for In-Vitro Biology. p. 122.Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen-(Peer Reviewed Journal) Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings-(Abstract Only) Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production-(Peer Reviewed Journal) Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system-(Peer Reviewed Journal) Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars-(Abstract Only) Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.Expression of a Heterologous Xylose Transporter in a Saccharomyces cerevisiae Strain Engineered to Utilize Xylose Improves Aerobic Xylose Co-consumption-(Peer Reviewed Journal) Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption. Applied Microbiology and Biotechnology. 80(4):675-684.Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol-(Abstract Only) Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain-(Abstract Only) Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption-(Abstract Only) Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption [abstract]. In: Proceedings of the 30th Symposium on Biotechnology for Fuels and Chemicals, May 4-7, 2008, New Orleans, LA. p. 131.The YMR315W gene from Saccharomyces cerevisiae codes for an alcohol dehydrogenase and is required for full resistance to oxidative stress-(Abstract Only)Removal of Fermentation Inhibitors from Alkaline Peroxide Pretreated and Enzymatically Hydrolyzed Wheat Straw:  Production of Butanol from Hydrolysate Using Clostridium beijerinckii in Batch Reactors-(Peer Reviewed Journal) Qureshi, N., Saha, B.C., Hector, R.E., Cotta, M.A. 2008. Removal of Fermentation Inhibitors from Alkaline Peroxide Pretreated and Enzymatically Hydrolyzed Wheat Straw:  Production of Butanol from Hydrolysate Using Clostridium beijerinckii in Batch Reactors. Biomass and Bioenergy. 32(12):1353-1358.Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain-(Peer Reviewed Journal) Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains-(Abstract Only) Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.ENGINEERING SACCHAROMYCES CEREVISIAE FOR ETHANOL PRODUCTION FROM AGRICULTURAL WASTE PRODUCTS-(Abstract Only) Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2007. Engineering Saccharomyces cerevisiae for ethanol production from agricultural waste products [abstract]. Yeast Cell Biology. Paper No. 176.BUTANOL PRODUCTION FROM WHEAT STRAW BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CLOSTRIDIUM BEIJERINCKII:  PART I-BATCH FERMENTATION-(Peer Reviewed Journal) Qureshi, N., Saha, B.C., Hector, R.E., Hughes, S.R., Cotta, M.A. 2008. Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii:  Part I - batch fermentation. Biomass and Bioenergy. 32:168-175.High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System-(Peer Reviewed Journal) Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides.-(Abstract Only) Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose expressing "stealth" insecticidal peptides [abstract]. American Chemical Society. p. 108.Fully automated molecular biology:  Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts.-(Abstract Only) Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy.-(Abstract Only) Riedmuller, S., Hughes, S.R., Hector, R.E., Bischoff, K.M., Farrelly, P., Cotta, M.A., Li, X. 2007. Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Seminar 43. p. 4.
 ARS News ArticlesitemStudies Steadily Advance Cellulosic Ethanol Prospects


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