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                    U.S. DEPARTMENT OF AGRICULTURE
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Douglas  Gladue

                                                                (PhD)
                        Foreign Animal Disease Research

                        




                        
                        Research Microbiologist
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                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsSupporting African Swine Fever and Classical Swine Fever Research
                                                    Cooperative Agreement
                                                                    (A)Accession Number: 445017Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine
                                                    In-House Appropriated
                                                                    (D)Accession Number: 441308African Swine Fever Collaborative Agreement
                                                    Non-Funded Cooperative Agreement
                                                                    (N)Accession Number: 440732Prediction of T-cell Epitopes for Lumpy Skin Disease Virus
                                                    Non-Funded Cooperative Agreement
                                                                    (N)Accession Number: 444540Analysis of the Cross Protective Response Induced by ASFV Recombinant Vaccine ASFV-G-DeltaI177L
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 444757Production of GMP-compliant Master Seed Virus (MSV) for Development of Vaccine Against African Swine Fever (ASF)
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 445010Curated African Swine Fever Genomic Database
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442398Development of a Differential ELISA for ASV Vaccines
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442399Development of an ASFV Nano-biosensor for Discriminating Between Infected and Vaccinated Animals
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442814Understanding Molecular Bases in the Production of Genetic Diversity of African Swine Fever Virus
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442906Predictive Structure of African Swine Fever Viral Proteins
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 433925Analysis of the Interaction Between Virus Proteins and Membrane Structures in Cells Infected with CSFV and ASFV
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 436252Actions Supporting the Development of an African Swine Fever Virus Live Attenuated DIVA Vaccine
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 436793Characterizing the Structure-Function of Various Families of Viroporins to Support African Swine Fever Virus Vaccine Candidates
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 438233Identification of Host Proteins That Interact with ASFV Proteins Through Yeast Two Hybrid Screening
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 438473Analysis of the Immune Response Elicited by Classical Swine Fever Virus Recombinant Vaccine Candidate FlagT4G
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 438599Functional Genomics of African Swine Fever Virus Infections - An Effective System to Identify Pro-Tolerance and Resilience Pathways in Pigs
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440094Evaluation of the Modification of Virulence in Recombinant ASFV with Specific Gene Deletions
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440174Development of a Self-amplifying mRNA Vaccine for African Swine Fever and Classical Swine Fever
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 440187**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)WSV2023 - The second meeting of the world society for virology: One health - One world - One virology

- (Peer Reviewed Journal)African swine fever virus p72 identification tool
                                                        

- (Other)Dinhobl, M.W., Spinard III, E.J., Birtley, H., Tesler, N., Masembe, C., Borca, M.V., Gladue, D.P. 2024. African swine fever virus p72 identification tool. Microbiology Resource Announcements. 13(2). Article e00891-23. https://doi.org/10.1128/mra.00891-23.ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies
                                                        

- (Peer Reviewed Journal)Borca, M.V., Ramirez Medina, E., Silva, E.B., Ayushi, R., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P. 2023. ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies. Pathogens. 12(6). https://doi.org/10.3390/pathogens12060805.Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
                                                        

- (Peer Reviewed Journal)Velazquez Salinas, L., Ramirez Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Espinoza, N.N., Gay, C.G., Witte, S.B., Gladue, D.P., Borca, M.V. 2023. Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines. Biologicals. 3;83:101685. https://doi.org/10.1016/j.biologicals.2023.101685.Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic
                                                        

- (Abstract Only)Ramirez Medina, E., O'Donell, V., Silva, E.B., Espinoza, N.N., Velazquez Salinas, L., Moran, K., Daite, D., Barrette, R., Bonto, F., Holland, R., Gladue, D.P., Borca, M.V. 2023. Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic. Meeting Abstract. https://doi.org/10.3390/v14051090.Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez Medina, E., Silva, E.B., Berggren, K., Rai, A., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2023. Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle. Viruses. 15(5). https://doi.org/10.3390/v15051036.Characterization of a novel African swine fever virus p72 genotype II from Nigeria
                                                        

- (Peer Reviewed Journal)Ambagala, A., Goonewardene, K., Lamboo, L., Goolia, M., Erdelyan, C., Fisher, M., Handel, K., Lung, O., Blome, S., King, J., Forth, J.H., Calvelage, S., Spinard Iii, E.J., Gladue, D.P., Masembe, C., Adedeji, A.J., Olubade, T., Maurice, N.A., Ularamu, H.G., Luka, P.D. 2023. Characterization of a novel African swine fever virus p72 genotype II from Nigeria. Viruses. https://doi.org/10.3390/v15040915.Development of porcine monoclonal antibodies with neutralizing activity against classical swine fever virus from C-strain E2 specific single B cells
                                                        

- (Peer Reviewed Journal)Wang, L., Madera, R., Li, Y., Gladue, D.P., Borca, M.V., McIntosh, M., Shi, J. 2023. Development of porcine monoclonal antibodies with neutralizing activity against classical swine fever virus from C-strain E2 specific single B cells. Viruses. 15(4);863. https://doi.org/10.3390/v15040863.Evaluation of the function of ASFV gene E66L in the process of virus replication and virulence in swine

- (Peer Reviewed Journal)Editorial: Insights of important mammalian viruses: Infection, pathogenesis and drugs
                                                        

- (Peer Reviewed Journal)Li, Y., Gladue, D.P., Li, S., Deng, L., Shen, C., Aguilar, P., Wang, T., Kong, L. 2023. Editorial: Insights of important mammalian viruses: Infection, pathogenesis and drugs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2023.1147109.Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019
                                                        

- (Peer Reviewed Journal)Hyeon, J., Tseren-Ochir, E., Lee, D., Gladue, D.P., Borca, M.V., Risatti, G.R. 2023. Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019. Viruses. 10. https://doi.org/10.3389/fvets.2023.1094052.Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980
                                                        

- (Peer Reviewed Journal)Spinnard, E., O'Donnell, V., Vuono, E., Davis, C., Rai, A., Ramirez Medina, E., Espinoza, N.N., Valadares, A., Borca, M.V., Gladue, D.P. 2023. Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980. Microbiology Resource Announcements. 13:1024(2023). https://doi.org/10.1038/s41598-022-25987-5.A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine
                                                        

- (Peer Reviewed Journal)Attreed, S.E., Silva, C.M., Abbott, S.T., Ramirez Medina, E., Espinoza, N.N., Borca, M.V., Gladue, D.P., Diaz San Segundo, F.C. 2022. A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine. Pathogens. 11(12). Article 1438. https://doi.org/10.3390/pathogens11121438.Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Spinard III, E.J., Valadares, A., Silva, E.B., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence. Viruses. 14(11). https://doi.org/10.3390/v14112548.The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates
                                                        

- (Peer Reviewed Journal)Silva, E.B., Krug, P., Ramirez Medina, E., Pruitt, S., Rai, A., Espinoza, N.N., Valadares, A., Gladue, D.P., Borca, M.V. 2022. The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates. Microbiology Resource Announcements. 11(11):1311. https://doi.org/10.3390/pathogens11111311.Structural predictions of the complete ASFV-G proteome
                                                        

- (Peer Reviewed Journal)Spinard III, E.J., Azzinaro, P.A., Rai, A., Espinoza, N.N., Ramirez Medina, E., Borca, M.V., Gladue, D.P. 2022. Structural predictions of the complete ASFV-G proteome. Microbiology Resource Announcements. 11(12):e0088122. https://doi.org/10.1128/mra.00881-22.The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against Classical swine fever
                                                        

- (Peer Reviewed Journal)Bohórquez, J.A., Wang, M., Diaz, I., Alberch, M., Perez-Simo, M., Rosell, R., Gladue, D.P., Borca, M.V., Llilianne, G. 2022. The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against classical swine fever. Viruses. 14(9). https://doi.org/10.3390/v14091954.ASFV Gene A151R is involved in the process of virulence in domestic swine
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Valladres, A., Spinard Iii, E.J., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. ASFV Gene A151R is involved in the process of virulence in domestic swine. Viruses. 14(8):1834. https://doi.org/10.3390/v14081834.Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs
                                                        

- (Abstract Only)Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Spinard Iii, E.J., Valladares, A., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Virus Research. https://doi.org/10.3390/v14081682.Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Viruses. 14(7):1409. https://doi.org/10.3390/v14071409.Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry
                                                        

- (Peer Reviewed Journal)Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Neilan, J., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry. Scientific Reports. https://doi.org/10.3390/v14061249.Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg
                                                        

- (Abstract Only)Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg. Meeting Abstract. https://doi.org/10.3390/v15040915.Deletion of H108R reduces virulence of the Georgia strain of African swine fever virus with surviving animals being protected against virulent challenge
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez Medina, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Valladares, A., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of H108R reduces virulence of the Georgia strain of African swine fever virus with surviving animals being protected against virulent challenge. Journal of Virology. https://doi.org/10.1128/jvi.00545-22.Experimental infection of domestic pigs with an African swine fever virus field strain isolated in 2021 from the Dominican Republic
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., O'Donnell, V., Silva, E.B., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Experimental infection of domestic pigs with an African swine fever virus field strain isolated in 2021 from the Dominican Republic. Viruses.  https://doi.org/10.3390/v14051090.Recombinant ASF live attenuated virus strains as experimental vaccine candidates
                                                        

- (Peer Reviewed Journal)Gladue, D.P., Borca, M.V. 2022. Recombinant ASF live attenuated virus strains as experimental vaccine candidates. Viruses. https://doi.org/10.3390/v14050878.Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L
                                                        

- (Peer Reviewed Journal)Xuan, T., Le Thi Thu, P., Nguyen Quang, H., Do Thanh, T., Nguyen Van, D., Pham Hào, Q., Quách Võ, N., Gay, C.G., Gladue, D.P., Borca, M.V. 2022. Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L. Viruses.  https://doi.org/10.3390/v14050896.Evaluation of an ASFV RNA helicase gene A859L for virus replication and swine virulence
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of an ASFV RNA helicase gene A859L for virus replication and swine virulence. Viruses. https://doi.org/10.3390/v14010010.World society for virology first international conference: tackling global virus epidemics
                                                        

- (Review Article)Soderlund-Venermo, M., Varma, A., Guo, D., Gladue, D.P., Poole, E., Pujol, F.H., Pappu, H., Romalde, J.L., Kramer, L., Baz, M., Venter, M., Moore, M.D., Nevels, M.M., Ezzikouri, S., Vakharia, V.N., Wilson, W.C., Malik, Y., Shi, Z., Abdel-Moneim, A.S. 2021. World society for virology first international conference: tackling global virus epidemics. Virology. 566(2022):114-121. https://doi.org/10.1016/j.virol.2021.11.009.Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors.
                                                        

- (Peer Reviewed Journal)Jaime, L., Lex, M., Gladue, D.P., Borca, M.V., Jorge, O. 2021. Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors. Scientific Reports. https://doi.org/10.1038/s41598-021-02949-x.Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines
                                                        

- (Abstract Only)Vuono, E., Ramirez Medina, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines. Meeting Abstract. https://doi.org/10.1128/JVI.01419-21.Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Pina-Pedrero, S., Zhu, J.J., Rodriguez, F., Borca, M.V., Gladue, D.P. 2021. Deletion of E184L, a putative DIVA target from the pandemic strain of African Swine Fever Virus, produces a reduction in virulence and protection against virulent challenge. Viruses. https://doi.org/10.1128/JVI.01419-21.Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs
                                                        

- (Peer Reviewed Journal)Bohórquez, J., Defaus, S.,  Pérez-Simó, M., Alberch, M., Gladue, D.P., Borca, M.V., Andreu, D., Ganges, L. 2021. Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs. Viruses. https://doi.org/10.3390/v13101980.Development real-time PCR assays to genetically differentiate vaccinated pigs from pigs infected with the Eurasian strain of African swine fever virus
                                                        

- (Peer Reviewed Journal)Velazquez Salinas, L., Ramirez Medina, E., Rai, A., Pruitt, S.E., Vuono, E.A., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2021. Development real-time PCR assays to genetically differentiate vaccinated pigs from pigs infected with the Eurasian strain of African swine fever virus. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2021.768869.African swine fever virus vaccine candidate ASFV-G-¿I177L efficiently protects European and native pig breeds against circulating Vietnamese field strain.
                                                        

- (Peer Reviewed Journal)Tran, X., Le Thi Thu, P., Nguyen Quang, H., Do Thanh, T., Nguyen Van, D., Gay, C.G., Borca, M.V., Gladue, D.P. 2021. African swine fever virus vaccine candidate ASFV-G-I177L efficiently protects European and native pig breeds against circulating Vietnamese field strain. Transboundary and Emerging Diseases. https://doi.org//10.1111/tbed.14329.Additional deletions to further attenuate BA71¿CD2 recombinant virus decreases vaccine efficacy without providing additional safety.

- (Peer Reviewed Journal)Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge
                                                        

- (Peer Reviewed Journal)Gladue, D.P., Ramirez Medina, E., Vuono, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez-Salinas, L., Borca, M.V. 2021. Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge. Journal of Virology.  https://doi.org/10.1128/JVI.01139-21.Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence. Viruses. https://doi.org/10.3390/v13060986.A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain
                                                        

- (Peer Reviewed Journal)Borca, M.V., Rai, A., Ramirez-Medina, E., Silva, E.B., Velazquez-Salinas, L., Vuono, E., Espinoza, N.N., Pruitt, S.E., Gladue, D.P. 2021. A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain. Nature Communications. https://doi.org/10.1128/JVI.00123-21.ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever
                                                        

- (Peer Reviewed Journal)Borca, M.V., Ramirez Medina, E., Silva, E., Vuono, E., Rai, A., Pruitt, S.E., Gay, C.G., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P. 2021. ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever. Viruses. https://doi.org/10.3390/v13050765.Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez-Media, E., Velazquez-Salinas, L., Berggren, K., Rai, A., Pruitt, S.E., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2021. Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle. Virology Journal. https://doi.org/10.1128/JVI.00314-21.Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene
                                                        

- (Peer Reviewed Journal)Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Valladares, A., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene. Viruses. https://doi.org/10.3390/v13020286.Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2021. Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene. Viruses. https://doi.org/10.3390/v13020286.Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez-Medina, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene. Viruses. https://doi.org/10.3390/v13010039.Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- ¿9GL abrogates its effectiveness as an experimental vaccine
                                                        

- (Peer Reviewed Journal)Gladue, D.P., O'Donnell, V., Ramierez-Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Silva, E., Velazquez-Salinas, L., Borca, M.V. 2020. Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- 9GL abrogates its effectiveness as an experimental vaccine. Viruses. https://doi.org/10.3390/v12101185.Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene
                                                        

- (Peer Reviewed Journal)Ramierez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Velazquez-Salinas, L., Zhu, J.J., Gladue, D.P., Borca, M.V. 2020. Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene. Viruses. https://doi.org/10.3390/v12101193.Positive Selection of ORF1ab, ORF3a, and ORF8 Genes Drives the Early Evolutionary Trends of SARS-CoV-2 During the 2020 COVID-19 Pandemic
                                                        

- (Peer Reviewed Journal)Velazquez-Salinas, L., Zarate, S., Eberl, S., Gladue, D.P., Novella, I., Borca, M.V. 2020. Positive selection of ORF1ab, ORF3a, and ORF8 genes drives the early evolutionary trends of SARS-CoV-2 during the 2020 COVID-19 pandemic. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.550674.X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E.A., Pruitt, S.E., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine. Viruses. https://doi.org/10.3390/v12060676.Residues within the foot-and-mouth disease virus 3dpol nuclear localization signal affect polymerase fidelity
                                                        

- (Peer Reviewed Journal)Kloc, A., Rai, D.K., Kenney, M.A., Schafer, E.A., Rieder, A.E., Gladue, D. 2020. Residues within the foot-and-mouth disease virus 3dpol nuclear localization signal affect polymerase fidelity. Journal of Virology. https://doi.org/10.1128/JVI.00833-20.A Single Amino Acid Substitution In The Matrix Protein (M51R) Of Vesicular Stomatitis New Jersey Virus Impairs Replication In Cultured Porcine Macrophages And Results In Significant Attenuation In Pigs
                                                        

- (Peer Reviewed Journal)Velazquez-Salinas, L., Pauszek, S.J., Holinka, L.G., Gladue, D.P., Rekant, S.I., Bishop, E.A., Stenfeldt, C., Verdugo-Rodriguez, A., Borca, M.V., Rodriguez, L.L., Arzt, J. 2020. A single amino acid substitution in the matrix protein (M51R) of Vesicular Stomatitis New Jersey virus impairs replication in cultured porcine macrophages and results in significant attenuation in pigs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.01123.SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine
                                                        

- (Peer Reviewed Journal)Vuono, E., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Borca, M.V., Gladue, D.P. 2020. SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine. Journal of Virology. https://doi.org/10.3390/v12040421.Detection and quantification of African swine fever in MA104 cells
                                                        

- (Peer Reviewed Journal)Rai, A., Pruitt, S.E., Ramierez-Medina, E., Vuono, E., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P., Borca, M.V. 2020. Detection and quantification of African swine fever in MA104 cells. Bio-protocol. https://doi.org/10.21769/BioProtoc.3955.Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus
                                                        

- (Peer Reviewed Journal)Vuonno, E., Ramirez-Media, E., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus. Viruses. https://doi.org/10.3390/v12040388.The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Silva, E., Rai, A., Pruitt, S.E., Vuono, E.A., Velazquez-Salinas, L., Berggren, K.A., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP. Viruses. https://doi.org/10.1128/JVI.02017-19.Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.
                                                        

- (Abstract Only)Borca, M.V., Ramirez Media, E., Silva, E., Vuono, E., Rai, A., Pruitt, S., Holinka, L., Velazquezsalinas, L., Gladue, D.P., Risatti, G., Shi, J. 2020.  Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.. Meeting Abstract. https://doi.org/10.1038/s41598-020-57455-3.First report describing the spatial-temporal circulation and the genetic diversity of Parapoxvirus species in Mexico
                                                        

- (Other)Velazquezsalinas, L., Ramirez Media, E., Bracht, A., Carrillo, C., Gladue, D.P. 2019. First report describing the spatial-temporal circulation and the genetic diversity of Parapoxvirus species in Mexico. Atlas of Science. https://doi.org/10.1016/j.meegid.2018.07.005.Mechanisms of African Swine Fever Virus Pathogenesis and Immune Evasion Inferred from Gene Expression Changes in Infected Swine Macrophages

- (Peer Reviewed Journal)Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine
                                                        

- (Peer Reviewed Journal)Borca, M.V., Vuono, E.A., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K.A., Singer, M., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P. 2019. Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine. Journal of Virology. https://doi.org/10.1128/JVI.01642-19.The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain
                                                        

- (Peer Reviewed Journal)Ramirez-Medina, E., Vuono, E., O'Donnell, V., Holinka, L.G., Silva, E., Rai, A., Pruitt, S., Carrillo, C., Gladue, D.P., Borca, M.V. 2019. The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain. Virology. https://doi.org/10.3390/v11070599.Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß)

- (Peer Reviewed Journal)Vuono, E., Ramirez-Medina, E., Holinka-Patterson, L.G., Baker-Branstetter, R., Borca, M.V., Gladue, D.P. 2019. Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß). Virology. Viruses 2019, 11(4), 307. https://doi.org/10.3390/v11040307.Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells
                                                        

- (Peer Reviewed Journal)Borca, M.V., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E., Berggren, K., Gladue, D.P. 2018. Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells. Virology. 526:38-44. https://doi.org/10.1016/j.virol.2018.10.004.Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B
                                                        

- (Peer Reviewed Journal)Gladue, D.P., Largo, E., De La Arada, I., Aguilella, V., Alcaraz, A., Arrondo, J., Holinka-Patterson, L.G., Brocchi, E., Ramirez-Medina, E., Vuono, E., Berggren, K., Carrillo, C., Borca, M.V. 2018. Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B. Journal of Virology. https://doi.org/10.1128/JVI.01360-18.Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum
                                                        

- (Peer Reviewed Journal)Gladue, D.P., Largo, E., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E.A., Berggren, K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2018. Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum. Virus Research. 10(9):E460. https://doi.org/10.3390/v10090460.CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus
                                                        

- (Peer Reviewed Journal)Borca, M.V., Berggren, K., Ramirez, E., Vuono, E., Gladue, D.P. 2018. CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus. Bio-protocol. 8(16). https://doi.org/10.21769/BioProtoc.2978.Phylodynamics of parapoxvirus genus in Mexico (2007–2011)
                                                        

- (Peer Reviewed Journal)Velazquez-Salinas, L., Ramirez-Medina, E., Bracht, A.J., Hole, K., Brito, B., Gladue, D.P., Carillo, C. 2018. Phylodynamics of parapoxvirus genus in Mexico (2007–2011). Infection, Genetics and Evolution. https://doi.org/10.1016/j.meegid.2018.07.005.The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß
                                                        

- (Peer Reviewed Journal)Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Ramirez, E., Vuono, E., Berggren, K., Alfano, M., Carey, L.B., Richt, J.A., Risatti, G.R., Gladue, D.P. 2018. The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß. Virus Research. 249:116-123. https://doi.org/10.1016/j.virusres.2018.03.017.CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses
                                                        

- (Peer Reviewed Journal)Borca, M.V., Holinka-Patterson, L.G., Berggren, K., Gladue, D.P. 2018. CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. Scientific Reports. 8(3154). https://doi.org/10.1038/s41598-018-21575-8.A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection
                                                        

- (Peer Reviewed Journal)Stenfeldt, C., Arzt, J., Pacheco, J., Gladue, D.P., Smoliga, G.R., Silva, E., Rodriguez, L.L., Borca, M.V. 2018. A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection. Virology. 516:115-126. https://doi.org/10.1016/j.virol.2018.01.008.Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses
                                                        

- (Peer Reviewed Journal)Largo, E., Gladue, D.P., Aguilella, V.M., Alcaraz, A., Borca, M.V., Nieva, J.L. 2018. Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses. Biochimica et Biophysica Acta. 1860:1015-1021. https://doi.org/10.1016/j.bbamem.2018.01.012.Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G

- (Peer Reviewed Journal)Holinka-Patterson, L.G., O'Donnell, V., Risatti, G., Azzinaro, P.A., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2017. Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One. doi: 10.1371/journal.pone.0177433.Development of a fluorescent ASFV strain that retains the ability to cause disease in swine

- (Peer Reviewed Journal)Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Sanford, B., Azzinaro, P.A., Risatti, G., Gladue, D.P. 2017. Development of a fluorescent ASFV strain that retains the ability to cause disease in swine. Nature Scientific Reports. 7:46747. doi: 10.1038/srep46747.Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain

- (Peer Reviewed Journal)O'Donnell, V., Risatti, G.R., Holinka-Patterson, L.G., Krug, P.W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P., Gladue, D.P., Borca, M.V. 2017. Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain. Journal of Virology. 91(1):e01760-16.Association of the host immune response with protection using a live attenuated African swine fever virus model
                                                        

- (Peer Reviewed Journal)Carlson, J., O'Donnell, V.K., Alfano, M., Velazquez-Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated African swine fever virus model  . Viruses. 8(10):E291. https://doi.org/10.3390/v8100291.Association of the host immune response with protection using a live attenuated African swine fever virus model

- (Peer Reviewed Journal)Carlson, J., O'Donnell, V., Alsfonso, M., Velazquez Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated african swine fever virus model. Viruses. 8(10):291. doi: 10.3390/v8100291.Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine

- (Peer Reviewed Journal)Holinka-Patterson, L.G., Largo, E., Gladue, D.P., O'Donnell, V.K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2016. Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine. Journal of Virology. 90:10299-10308. doi: 10.1128/JVI.01530-16.The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6
                                                        

- (Peer Reviewed Journal)Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Rai, D., Sanford, B., Alfano, M., Carlson, J., Azzinaro, P., Alonso, C., Gladue, D.P. 2016. The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6. Virus Research. 223:181-189. doi: 10.1016/j.virusres.2016.07.013.Selective factors associated with the evolution of codon usage in natural populations of arboviruses and their practical application to infer possible hosts for emerging viruses

- (Peer Reviewed Journal)Velazquez-Salinas, L., Zarate, S., Eschbaumer, M., Lobo, F.P., Gladue, D.P., Arzt, J., Novella, I.S., Rodriguez, L.L. 2016. Selective factors associated with the evolution of codon usage in natural populations of arboviruses and their practical application to infer possible hosts for emerging viruses. PLoS One. PLoS ONE 11(7): e0159943. doi:10.1371/journal.pone.0159943.African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge

- (Peer Reviewed Journal)O'Donnell, V.K., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B., Krug, P.W., Carlson, J., Pacheco, J., Reese, B., Risatti, G.R., Borca, M.V. 2016. African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge. Virology. 221:8-14. doi: 10.1016/j.virusres.2016.05.014.Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease

- (Peer Reviewed Journal)Velazquez-Salinas, L., Risatti, G.R., Holinka-Patterson, L.G., O'Donnell, V.K., Carlson, J., Alfano, M., Rodriguez, L.L., Carrillo, C., Gladue, D.P., Borca, M.V. 2016. Recoding classical swine fever virus (CSFV) structural glycoprotein E2 produces complete virus attenuation in swine and protects infected animals against disease. Virology. 494:178-189. doi: 10.1016/j.virol.2016.4.007.Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus

- (Peer Reviewed Journal)Sandford, B., Holinka-Patterson, L.G., O'Donnell, V., Krug, P.W., Carlson, J., Alfano, M., Carrillo, C., Wu, P., Lowe, A., Risatti, G.R., Gladue, D.P., Borca, M.V. 2015. Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Research. 213:165-171. doi: 10.1016/j.viruses.2015.12.002.Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine

- (Peer Reviewed Journal)Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Risatti, G.R., Borca, M.V. 2011. Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine. Virology. 412:68-74.Identification of an NTPase motif in classical swine fever virus NS4B protein

- (Peer Reviewed Journal)Gladue, D.P., Gavrilov, B.K., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., Vepkhvadze, N.G., Rogers, K., O'Donnell, V., Borca, M.V. 2011. Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology. 411(1):41-49.Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine

- (Peer Reviewed Journal)Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Saniz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Rogers, K., Risatti, G.R., Borca, M.V. 2010. Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine. Virology. 407:129-136.Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine

- (Peer Reviewed Journal)Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gladue, D.P., O'Donnell, V., Lu, Z., Gavrilov, B.K., Risatti, G.R., Borca, M.V. 2011. Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine. Journal of Virology. 407:129-136.Mutations in the classical swine fever virus NS4B protein affects virulence in swine

- (Abstract Only)Fernandez Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Zhiqiang, L., Risatti, G.R., Zhu, J.J., Borca, M.V. 2009. Mutations in the classical swine fever virus NS4B protein affects virulence in swine. Proceedings. P. 143.Mutations in classical swine fever virus NS4B affect virulence in swine

- (Peer Reviewed Journal)Fernandez-Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Lu, Z., Risatti, G.R., Zhu, J.J., Borca, M.V. 2010. Mutations in classical swine fever virus NS4B affect virulence in swine. Journal of Virology. 84(3):1536-1549.Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine

- (Abstract Only)Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., Lu, Z., Borca, M.V. 2009. Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine. American Society for Virology Meeting.  Paper No. W49-5: P. 213.Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine

- (Peer Reviewed Journal)Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gavrilov, B.K., Prarat, M.V., Gladue, D.P., Lu, Z., Jia, W., Risatti, G.R., Borca, M.V. 2009. Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine. Virology. 386:210-216.Development of a live attenuated antigenic marker classical swine fever vaccine

- (Peer Reviewed Journal)Holinka-Patterson, L.G., Fernandez-Sainz, I., O'Donnell, V., Prarat, M.V., Gladue, D.P., Lu, Z., Risatti, G., Borca, M.V. 2008. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology. 384:106-113.Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray

- (Peer Reviewed Journal)Gladue, D.P., Zhu, J.J., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Borca, M.V. 2010. Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virology. 138(1-2):89-96.
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