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                    U.S. DEPARTMENT OF AGRICULTURE
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ARS Home Southeast Area New Orleans, Louisiana Southern Regional Research Center Commodity Utilization Research People & Locations







Jay M Shockey
                        Commodity Utilization Research

                        




                        
                        Research Geneticist (Plants)
                        Phone: (504) 286-4296
                        
                        
                        Fax: (504) 286-4419(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsCreation of Enhanced-Value Cotton Germplasm via Modulation of Cyclopropyl Fatty Acid Accumulation
                                                    Cooperative Agreement
                                                                    (A)Accession Number: 445640Development of Novel Cottonseed Products and Processes
                                                    In-House Appropriated
                                                                    (D)Accession Number: 438713Creation of Enhanced-Value Cotton Germplasm via Modulation of Cyclopropyl Fatty Acid Accumulation
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 441777Beyond Static Metabolic Maps - Understanding the Cellular Organization and Dynamics of Lipid Flux for Enhanced Seed Oil Production
                                                    Reimbursable Cooperative Agreement
                                                                    (R)Accession Number: 444185**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils
                                                        

- (Peer Reviewed Journal)Parchuri, P., Bhandari, S., Azeez, A., Chen, G., Johnson, K., Shockey, J., Smertenko, A., Bates, P.D. 2024. Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils. Nature Communications. 15. Article 3547. https://doi.org/10.1038/s41467-024-47995-x.Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm.
                                                        

- (Peer Reviewed Journal)Dowd, M.K., Shockey, J., Mccarty Jr, J.C., Jenkins, J.N. 2023. Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm. Crop Science. 63(6):3393-3401. https://doi.org/10.1002/csc2.21119.A mutant cotton fatty acid desaturase 2-1d allele causes protein mistargeting and altered seed oil composition
                                                        

- (Peer Reviewed Journal)Shockey, J.M., Gilbert, M.K., Thyssen, G.N. 2023. A mutant cotton fatty acid desaturase 2-1d allele causes protein mistargeting and altered seed oil composition. BMC Plant Biology. 23. Article 147. https://doi.org/10.1186/s12870-023-04160-8.Assessing the biotechnological potential of cotton type-1 and type-2 diacylglycerol acyltransferases in transgenic systems
                                                        

- (Peer Reviewed Journal)Shockey, J., Parchuri, P., Thyssen, G.N., Bates, P.D. 2023. Assessing the biotechnological potential of cotton type-1 and type-2 diacylglycerol acyltransferases in transgenic systems. Plant Physiology and Biochemistry. 196:940-951. https://doi.org/10.1016/j.plaphy.2023.02.040.Biochemical properties of Acyl-CoA-dependent and Acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols
                                                        

- (Peer Reviewed Journal)Rahman, M., Klunga, J., Behera, J., Shockey, J.M., Kilaru, A. 2023. Biochemical properties of Acyl-CoA-dependent and Acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols. Frontiers in Plant Science.  https://doi.org/10.3389/fpls.2022.1056582.A cottonseed oil-enriched diet improves liver and plasma lipid levels in a male mouse model of fatty liver
                                                        

- (Peer Reviewed Journal)Son, Y., Shockey, J., Dowd, M.K., Shieh, J.G., Cooper, J.A., Paton, C.M. 2023. A cottonseed oil-enriched diet improves liver and plasma lipid levels in a male mouse model of fatty liver. American Journal of Physiology - Regulatory Integrative & Comparative Physiology. 324: R171–R182 https://doi.org/10.1152/ajpregu.00052.2022.Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21
                                                        

- (Peer Reviewed Journal)Zhang, L., Wu, P., Li, W., Feng, T., Shockey, J., Chen, L., Zhang, L., Lu, S. 2021. Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21. The Plant Journal. 15540. 108(6):1735-1753. https://doi.org/10.1111/tpj.Functional and predictive structural characterization of WRINKLED2, a unique oil biosynthesis regulator in avocado
                                                        

- (Peer Reviewed Journal)Behera, J.R., Rahman, M.M., Bhatia, S., Shockey, J., Kilaru, A. 2021. Functional and predictive structural characterization of WRINKLED2, a unique oil biosynthesis regulator in avocado. Frontiers in Plant Science. 12:648494. https://doi.org/10.3389/fpls.2021.648494.Oil-producing metabolons containing DGAT1 use separate substrate pools from those containing DGAT2 or PDAT
                                                        

- (Peer Reviewed Journal)Regmi, A., Shockey, J., Kotapati, H.K., Bates, P.D. 2020. Oil-producing metabolons containing DGAT1 use separate substrate pools from those containing DGAT2 or PDAT. Plant Physiology. 184(2):720-737. https://doi.org/10.1104/pp.20.00461.Gene editing in plants: assessing the variables through a simplified case study
                                                        

- (Peer Reviewed Journal)Shockey, J. 2020. Gene editing in plants: assessing the variables through a simplified case study. Plant Molecular Biology. 103:75-89. https://doi.org/10.1007/s11103-020-00976-2.Registration of four upland cotton germplasm lines with elevated levels of seed oil oleic acid
                                                        

- (Peer Reviewed Journal)Dowd, M.K., McCarty Jr, J.C., Shockey, J., Jenkins, J.N. 2020. Registration of four upland cotton germplasm lines with elevated levels of seed oil oleic acid. Journal of Plant Registrations. 14(1):64-71. https://doi.org/10.1002/plr2.20017.Gene editing in cotton for oil improvement

- (Abstract Only)Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils
                                                        

- (Peer Reviewed Journal)Shockey, J., Lager, I., Stymne, S., Kotapati, H.K., Sheffield, J., Mason, C., Bates, P.D. 2019. Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils. Planta. 1-15. https://doi.org/10.1007/s00425-018-03086-y.Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes
                                                        

- (Peer Reviewed Journal)Shockey, J., Kuhn, D., Chen, T., Cao, H., Freeman, B., Mason, C. 2018. Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes. Plant Cell Reports. 37(11):1571-1583. https://doi.org/10.1007/s00299-018-2329-y.Tung tree (Vernicia fordii, Hemsl.) genome and transcriptome sequencing reveals co-ordinate up-regulation of fatty acid beta-oxidation and triacylglycerol biosynthesis pathways during eleostearic acid accumulation in seeds
                                                        

- (Peer Reviewed Journal)Cui, P., Lin, Q., Fang, D., Zhang, L., Li, R., Cheng, J., Gao, F., Shockey, J., Hu, S., Lu, S. 2018. Tung tree (Vernicia fordii, Hemsl.) genome and transcriptome sequencing reveals co-ordinate up-regulation of fatty acid beta-oxidation and triacylglycerol biosynthesis pathways during eleostearic acid accumulation in seeds. Plant And Cell Physiology. 59:1990-2003. https://doi.org/10.1093/pcp/pcy117.Discovery of a new mechanism for regulation of plant triacylglycerol metabolism: The peanut diacylglycerol acyltransferase-1 gene family transcriptome is highly enriched in alternative splicing variants

- (Peer Reviewed Journal)Zheng, L., Shockey, J., Guo, F., Shi, L., Li, X., Shan, L., Wan, S., Peng, Z. 2017. Discovery of a new mechanism for regulation of plant triacylglycerol metabolism: The peanut diacylglycerol acyltransferase-1 gene family transcriptome is highly enriched in alternative splicing variants. Journal of Plant Physiology. 219:62-70.Variant amino acid residues alter the enzyme activity of peanut type 2
Diacylglycerol Acyltransferases

- (Peer Reviewed Journal)Zheng, L., Shockey, J., Bian, F., Chen, G., Shan, L., Li, X., Wan, S., Peng, Z. 2017. Variant amino acid residues alter the enzyme activity of peanut type 2 diacylglycerol acyltransferases. Frontiers in Plant Science. 8:1751.Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

- (Peer Reviewed Journal)Yurchenko, O., Shockey, J.M., Gidda, S.K., Silver, M.L., Chapman, K.D., Mullen, R.T., Dyer, J.M. 2017. Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves. Plant Biotechnology Journal. 15:1010-1023. doi: 10.111/pbi.12695.Naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of Gossypium barbadense fatty acid desaturase-2

- (Peer Reviewed Journal)Shockey, J., Dowd, M., Mack, B., Gilbert, M., Scheffler, B., Ballard, L., Frelichowski, J., Mason, C. 2017. Naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of Gossypium barbadense fatty acid desaturase-2. Planta. 245:611-622.Proteomic analysis of tung tree (Vernicia fordii) oilseeds during the developmental stages

- (Peer Reviewed Journal)Zhan, Z., Chen, Y., Shockey, J., Han, X., Wang, Y. 2016. Proteomic analysis of tung tree (Vernicia fordii) oilseeds during the developmental stages. Molecules. 21:1486.Dedicated industrial oilseed crops as metabolic engineering platforms for sustainable industrial feedstock production

- (Peer Reviewed Journal)Zhu, L.-H., Krens, F., Smith, M.A., Li, X., Qi, W., Van Loo, E.N., Iven, T., Feussner, I., Nazarenus, T.J., Huai, D., Taylor, D.C., Zhou, X.-R., Green, A.G., Shockey, J., Klasson, K.T., Mullen, R.T., Huang, B., Dyer, J.M., Cahoon, E.B. 2016. Dedicated industrial oilseed crops as metabolic engineering platforms for sustainable industrial feedstock production. Scientific Reports. 6:22181.Tung (Vernicia fordii and Vernicia montana)

- (Book / Chapter)Shockey, J., Rinehart, T., Chen, Y., Yangdong, W., Zhiyong, Z., Lisong, H. 2016. Tung (Vernicia fordii and Vernicia montana). In: McKeon, T.A., Hayes, D.G., Hildebrand, D.F., Weselake, R.J., editors. Industrial Oil Crops. Kidlington, Oxford, UK: Academic Press and AOCS Press. p. 243-273.Identification of Arabidopsis GPAT9 (At5g60620) as an essential gene involved in Triacylglycerol Biosynthesis

- (Peer Reviewed Journal)Shockey, J., Regmi, A., Cotton, K., Adhikari, N., Browse, J., Bates, P.D. 2015. Identification of Arabidopsis GPAT9 (At5g60620) as an essential gene involved in Triacylglycerol Biosynthesis. Plant Physiology. 170:163-179.Vernicia fordii ‘Spiers’, a new tung tree for commercial tung oil production in the gulf coast region

- (Peer Reviewed Journal)Rinehart, T.A., Shockey, J.M., Edwards Jr, N.C., Spiers, J.M., Klasson, K.T. 2015. Vernicia fordii ‘Spiers’, a new tung tree for commercial tung oil production in the gulf coast region. HortScience. 50: 1830-1832.Development and analysis of a highly flexible multi-gene expression system for metabolic engineering in Arabidopsis seeds and other plant tissues
                                                        

- (Peer Reviewed Journal)Shockey, J., Mason, C., Gilbert, M., Cao, H., Li, X., Cahoon, E., Dyer, J. 2015. Development and analysis of a highly flexible multi-gene expression system for metabolic engineering in Arabidopsis seeds and other plant tissues. Plant Molecular Biology. 89(1-2):113-126.Reducing isozyme competition increases target fatty acid accumulation in seed triacylglycerols of transgenic Arabidopsis

- (Peer Reviewed Journal)Van Erp, H., Shockey, J., Zhang, M., Adhikari, N.D., Browse, J. 2015. Reducing isozyme competition increases target fatty acid accumulation in seed triacylglycerols of transgenic Arabidopsis. Plant Physiology. 168:36-46.Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii)
                                                        

- (Peer Reviewed Journal)Cao, H., Zhang, L., Tan, X., Long, H., Shockey, J.M. 2014. Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii). PLoS One. 9(2):e88409.Plant acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) have different specificities in their forward and reverse reactions

- (Peer Reviewed Journal)Lager, I., Yilmaz, J., Zhou, X., Jasieniecka, K., Kazachkov, M., Wang, P., Zou, J., Weselake, R., Smith, M.A., Bayon, S., Dyer, J.M., Shockey, J.M., Heinz, E., Green, A., Banas, A., Stymne, S. 2013. Plant acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) have different specificities in their forward and reverse reactions. Journal of Biological Chemistry. 288(52):36902-36914.Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues

- (Peer Reviewed Journal)Cao, H., Shockey, J.M., Klasson, K.T., Chapital, D.C., Mason, C.B., Scheffler, B.E. 2013. Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues. PLoS ONE 8(10):e76946. doi: 10.1371/journal.pone.0076946.A transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (UGT73C14) induced in developing fiber of Ligon lintless-2 mutant of cotton (Gossypium hirsutum L.)

- (Peer Reviewed Journal)Gilbert, M.K., Bland, J.M., Shockey, J.M., Cao, H., Hinchliffe, D.J., Fang, D.D., Naoumkina, M.A. 2013. A transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (UGT73C14) induced in developing fiber of Ligon lintless-2 mutant of cotton (Gossypium hirsutum L.). PLoS One. 8(9):e75268.A transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (UGT73C14) induced in developing fiber of Ligon lintless-2 mutant of cotton (Gossypium hirsutum L.)

- (Peer Reviewed Journal)Gilbert, M.K., Bland, J.M., Shockey, J.M., Cao, H., Hinchliffe, D.J., Fang, D.D., Naoumkina, M.A. 2013. A transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (UGT73C14) induced in developing fiber of Ligon lintless-2 mutant of cotton (Gossypium hirsutum L.). PLoS One. 8(9):e75268.Assessment of the tung tree (Vernicia fordii) germplasm collection and latest cultivar release at the USDA-ARS Southern Horticultural Research Unit in Poplarville, MS

- (Abstract Only)Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii)

- (Peer Reviewed Journal)Pastor, S., Sethumadhavan, K., Ullah, A.H., Gidda, S., Cao, H., Mason, C., Chapital, D., Scheffler, B., Mullen, R., Dyer, J., Shockey, J. 2013. Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii). Plant Science. 203-204:79-88.Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues

- (Peer Reviewed Journal)Cao, H., Shockey, J.M. 2012. Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues. Journal of Agricultural and Food Chemistry. 60(50):12296-12303.Measuring phosphatidic acid phosphatase (EC 3.1.3.4) activity using two phosphomolybdate-based colorimetric methods

- (Peer Reviewed Journal)Ullah, A.H.J., Sethumadhavan, K., Shockey, J. 2012. Measuring phosphatidic acid phosphatase (EC 3.1.3.4) activity using two phosphomolybdate-based colorimetric methods. Advances in Biological Chemistry. 2:416-421.Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria

- (Peer Reviewed Journal)Ullah, A.H.J., Sethumadhavan, K., Grimm, C., Shockey, J. 2012. Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria. Advances in Biological Chemistry.  2(4):403-410.Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria

- (Peer Reviewed Journal)Ullah, A.H.J., Sethumadhavan, K., Grimm, C., Shockey, J. 2012. Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria. Advances in Biological Chemistry. 2(4):403-410.Breeding status of tung tree (Vernicia sp.) in China, a multipurpose oilseed crop with industrial uses

- (Peer Reviewed Journal)Zhan, Z., Wang, Y., Shockey, J., Chen, Y., Zhou, Z., Yao, X., Ren, H. 2012. Breeding status of tung tree (Vernicia sp.) in China, a multipurpose oilseed crop with industrial uses. Silvae Genetica. 61(6):265-270.Long chain acyl-CoA synthetases and other acyl activating enzymes

- (Other)Shockey, J. 2012. Long chain acyl-CoA synthetases and other acyl activating enzymes. In: Harwood, J.L., Weselake, R.J., editors. The AOCS Lipid Library [online]. Urbana, IL: American Oil Chemist’s Society. Available: http://lipidlibrary.aocs.org/plantbio/coAsynthetases/index.htmExpression and purification of recombinant tung tree diacylglycerol acyltransferase 2

- (Peer Reviewed Journal)Cao, H., Chapital, D.C., Howard Jr, O.D., Deterding, L.J., Mason, C.B., Shockey, J.M., Klasson, K.T. 2012. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2. Applied Microbiology and Biotechnology. 96(3):711-727.Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2
                                                        

- (Peer Reviewed Journal)Cao, H., Chapital, D.C., Howard Jr, O.D., Deterding, L.J., Mason, C.B., Shockey, J.M., Klasson, K.T. 2012. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2. Applied Microbiology and Biotechnology. 96(3):711-727.Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae

- (Peer Reviewed Journal)Shockey, J., Chapital, D., Gidda, S., Mason, C., Davis, G., Klasson, K.T., Cao, H., Mullen, R., Dyer, J. 2011. Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 92(6):1207-1217.Expression of tung tree diacylglycerol acyltransferase 1 in E. coli

- (Peer Reviewed Journal)Cao, H., Chapital, D.C., Shockey, J.M., Klasson, K.T. 2011. Expression of tung tree diacylglycerol acyltransferase 1 in E. coli. BMC Biotechnology. 11:73 (13 pages).Bioengineering recombinant tung tree diacylglycerol acyltransferases

- (Abstract Only)Cao, Heping, Chapital, Dorselyn C., Howard, Jr., O.D., Shockey, Jay M., Klasson, K. Thomas. 2011. Bioengineering recombinant tung tree diacylglycerol acyltransferases (abstract). Presented at the Society for Industrial Microbiology and Biotechnology Annual Meeting and Exhibition in New Orleans, Louisiana, July 24-28. http://sim.confex.com/sim/2011/webprogram/Paper19213.html.Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2

- (Abstract Only)Howard, O., Chapital, D.C., Shockey, J.M., Cao, H. 2011. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 (abstract). Southern Section of the American Society of Plant Biologists. p. 5.Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli

- (Abstract Only)Cao, H., Chapital, D.C., Howard Jr, O.D., Jiang, X., Shockey, J.M., Klasson, K.T. 2011. Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli (abstract). Journal of the Federation of American Societies for Experimental Biology. 25:765.8.Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants

- (Peer Reviewed Journal)Shockey, J., Browse, J. 2011. Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants. Plant Journal. (66):143-160.Hydrophobic-domain-dependent protein-protein interactions mediate the localization of GPAT enzymes to ER subdomains

- (Peer Reviewed Journal)Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. 2011. Hydrophobic-domain-dependent protein-protein interactions mediate the localization of GPAT enzymes to ER subdomains. Traffic. 12:452-472.Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis

- (Peer Reviewed Journal)Van Erp, H., Bates, P.D., Burgal, J., Shockey, J., Browse, J. 2011. Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis. Plant Physiology. 155:683-693.The N termini of Brassica and tung omega-3 fatty acid desaturases mediate proteasome-dependent protein degradation in plant cells

- (Peer Reviewed Journal)Khuu, N., Gidda, S., Shockey, J.M., Dyer, J.M., Mullen, R.T., 2011. The N termini of Brassica and tung omega-3 fatty acid desaturases mediate proteasome-dependent protein degradation in plant cells. Plant Signaling and Behavior. 6(3):422-425.Mining the bitter melon (momordica charantia l.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes

- (Peer Reviewed Journal)Yang, P., Li, X., Shipp, M.J., Shockey, J.M., Cahoon, E.B. 2010. Mining the bitter melon (momordica charantia l.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes. Biomed Central (BMC) Plant Biology. 10:250.Transmembrane domain-dependent protein-protein interactions participate in the localization of GPAT enzymes to ER subdomains

- (Abstract Only)Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., and Mullen, R.T. (2010). Transmembrane domain-dependent protein-protein interactions participate in the localization of GPAT enzymes to ER subdomains.  Joint Annual Meeting of the American Society of Plant Biologists & the Canadian Society of Plant Physiologists, July 31 - August 4, 2010, Montreal, Canada.Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast

- (Abstract Only)Cao, H., Chapital, D.C., Mason, C.B., Shockey, J.M., Klasson, K.T. 2010. Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast (abstract). Plant Biology 2010, July 31-August 4, Montreal, Canada.Temperature-sensitive, Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway

- (Peer Reviewed Journal)O'Quin, J.B., Bourassa, L., Zhang, D., Shockey, J.M., Gidda, S.K.,  Fosnot, S., Chapman, K.D., Mullen, R.T., Dyer, J.M. 2010. Temperature-sensitive, Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway. Journal of Biological Chemistry. 285(28):21781-21796.Biogenesis of ER subdomains containing DGAT2, an enzyme involved in industrial oil biosynthesis

- (Abstract Only)Gidda, S.K., Shockey, J.M., Mullen, R.T., and Dyer, J.M. (2010). Biogenesis of ER subdomains containing DGAT2, an enzyme involved in industrial oil biosynthesis. Annual Meeting of the American Oil Chemists' Society, May 11 - 16, 2010, Phoenix, AZ.TEMPERATURE-SENSITIVE, POST-TRANSLATIONAL REGULATION OF  PLANT OMEGA-3 FATTY ACID DESATURASES IS MEDIATED BY THE ER-ASSOCIATED DEGRADATION PATHWAY

- (Abstract Only)Zhang, D., Quin, J.B., Bourassa, L., Foss, S., Shockey, J.M., Gidda, S.K., Chapman, K.D., Mullen, R.T., and Dyer, J.M. (2010). Temperature-sensitive, post-translational regulation of plant omega-3 fatty acid desaturases is mediated by the ER-associated degradation pathway.  Annual Meeting of the American Society of Biochemistry and Molecular Biology, April 24 - 28, 2010, Anaheim, CA.Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis

- (Peer Reviewed Journal)Weng, H., Molina, I., Shockey, J., Browse, J. 2010. Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis. Planta. 231(5):1089-1100.Biogenesis of glycerol 3-phosphate acyltransferase (GPAT): influence of transmembrane domains and protein-protein interactions on the localization of GPAT to ER subdomains

- (Abstract Only)Gidda, S.K., Shockey, J.M., Dyer, J.M., and Mullen, R.T. (2009). Biogenesis of glycerol 3-phosphate acyltransferase (GPAT): influence of transmembrane domains and protein-protein interactions on the localization of GPAT to ER subdomains.  Eastern Regional Meeting of the Canadian Society of Plant Physiologists, December 4 - 5, 2009, Guelph, Ontarion, Canada.Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells

- (Peer Reviewed Journal)Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. (2009). Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiology and Biochemistry, 47:867-879.Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: Functional divergence of the dilysine ER retrieval motif in plant cells

- (Peer Reviewed Journal)Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. 2009. Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: Functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiology and Biochemistry.  47(10):867-879.Engineering industrial oil biosynthesis: cloning and characterization of Kennedy pathway acyltransferases from novel oilseed species

- (Book / Chapter)Shockey, J.M. 2009. Engineering industrial oil biosynthesis: cloning and characterization of Kennedy pathway acyltransferases from novel oilseed species. In: Hou, T.H., Shaw, J.-F., editors. Biocatalysis and Agricultural Biotechnology. Abingdon, England:CRC Press. pp. 19-31.Metabolic engineering of hydroxy fatty acid production in plants: rcdgat2 drives dramatic increases in ricinoleate levels in seed oil

- (Peer Reviewed Journal)Burgal, J., Shockey, J., Lu, C., Dyer, J., Larson, T., Graham, I., Browse, J. 2008. Metabolic engineering of hydroxy fatty acid production in plants: rcdgat2 drives dramatic increases in ricinoleate levels in seed oil.  Plant Biotechnology Journal. 6(8):819-831.Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux

- (Review Article)Cahoon, E.B., Shockey, J.M., Dietrich, C.R., Dyer, J.M. 2007. Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Current Opinion in Plant Biology. 10:236-244.TUNG TREE DGAT1 AND DGAT2 HAVE NONREDUDANT FUNCTIONS IN TRIACYLGLYCEROL BIOSYNTHESIS AND ARE LOCALIZED TO DIFFERENT SUBDOMAINS OF THE ENDOPLASMIC RETICULUM

- (Peer Reviewed Journal)Shockey, J.M., Gidda, S., Chapital, D.C., Kuan, J.W., Dhanoa, P.K., Bland, J.M., Rothstein, S.J., Mullen, R.T., Dyer, J.M. 2006. Tung tree DGAT1 and DGAT2 have nonredudant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. The Plant Cell 18:2294-2313.CHARACTERIZATION IN VITRO AND IN VIVO OF THE PUTATIVE MULTIGENE 4-COUMARATE:COA LIGASE NETWORK IN ARABIDOPSIS: SYRINGYL LIGNIN AND SINAPATE/SINAPYL ALCOHOL DERIVATIVE FORMATION.

- (Peer Reviewed Journal)Costa, M.A., Bedgar, D.L., Moinuddin, S.G., Kim, K.W., Cardenas, C.L., Cochrane, F.C., Shockey, J.M., Helms, G.L., Amakura, Y., Takahashi, H., Milhollan, J.K., Davin, L.B., Browse, J., Lewis, N.G. 2005. Characterization in vitro and in vivo of the putative multigene 4-coumarate:coa ligase network in arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation. Phytochemistry.  66:2072-2091.ER-LOCALIZED OMEGA-3 FATTY ACID DESATURASES ARE SHORT-LIVED PROTEINS THAT CONTAIN DEGRADATION SIGNALS IN THEIR N TERMINI

- (Abstract Only)Dyer, J.M., Bryan, J., Bourassa, L., Shockey, J.M., and Mullen, R.T. 2005. ER-localized omega-3 fatty acid desaturases are short-lived proteins that contain degradation signals in their N termini (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract A3, page 6, June 10-14, Fallen Leaf Lake, CA.  2005:17TYPE 1 AND TYPE 2 DIACYLGLYCEROL ACYLTRANSFERASES FROM DEVELOPING  SEEDS OF TUNG (VERNICIA FORDII) DISPLAY DIFFERENT AFFINITIES FOR ELEOSTEARIC ACID-CONTAINING SUBSTRATES.

- (Abstract Only)Shockey, J.M., Gidda, S., Chapital, D.C., Kuan, J.-C.W., Rothstein, S.J., Mullen, R.T., and Dyer, J.M. 2005. Type 1 and type 2 diacylglycerol acyltransferases from developing seeds of tung (Vernicia fordii) display different affinities for eleostearic acid-containing substrates (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract C3, page 11, June 10-14, Fallen Leaf Lake, CA.  2005:22CLONING, FUNCTIONAL ANALYSIS, AND SUBCELLULAR LOCALIZATION OF TWO ISOFORMS OF NADH:CYTOCHROME B5 REDUCTASE FROM DEVELOPING SEEDS OF TUNG (VERNICIA FORDII)

- (Peer Reviewed Journal)Shockey, J.M., Dhanoa, P.K., Dupuy, T., Chapital, D.C., Mullen, R.T., and Dyer, J.M. 2005. Cloning, functional analysis, and subcellular localization of two isoforms of NADH:cytochrome b5 reductase from developing seeds of tung (Vernicia fordii). Plant Science. 169(2):375-385.BIOTRANSFORMATION OF LIPIDS IN YEAST CELLS: ENZYMATIC CONVERSION OF FATTY ACIDS INTO OMEGA-3 AND CONJUGATED FATTY ACIDS FOR POTENTIAL USAGE AS NUTRACEUTICALS

- (Proceedings)Dyer, J.M., Shockey, J.M., Mullen, R.T., Chapital, D.C., Kuan, J.-C., and Stewart, G. (2004). Biotransformation of lipids in yeast cells: enzymatic conversion of fatty acids into omega-3 and conjugated fatty acids for potential usage as nutraceuticals. In: Proceedings of the 33rd Annual United States-Japan Cooperative Program in Natural Resources Annual Meeting, December 11, 2004, Tokyo, JA. p. 210-214.CLONING AND CHARACTERIZATION OF A LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE FROM TUNG (ALEURITIES FORDII)

- (Abstract Only)Shockey, J.M., Dyer, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Cary, J.W.  2004.  Cloning and characterization of a lysophosphatidic acid acyltransferase from tung (aleurities fordii)(abstract).  American Society of Microbiology.BIOSYNTHESES OF TRIACYLGLYCEROLS CONTAINING ELEOSTEARIC ACID. CLONING AND CHARACTERIZATION OF THE QUANTITATIVE AND QUALITATIVE ROLES OF DIACYLGLYCEROL ACYLTRANSFERASE AND PHOSPHOLIPID-DIACYLGLYCEROL ACYLTRANSFERASE FROM TUNG

- (Abstract Only)Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dupuy, T., Dyer, J.M. 2004. Biosyntheses of triacylglycerols containing eleostearic acid. cloning and characterization of the quantitative and qualitative roles of diacylglycerol acyltransferase and phospholipid-diacylglycerol acyltransferase from tung (abstract).  American Society of Microbiology.ENGINEERING HIGH LEVELS OF ELEOSTEARIC ACID CONTENT IN OILSEEDS - CLONING AND FUNCTIONAL CHARACTERIZATION OF TRIACYLGLYCEROL BIOSYNTHETIC ACYLTRANSFERASE GENES FROM TUNG (ALEURITES FORDII)

- (Abstract Only)Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dyer, J.M. 2004. Engineering high levels of eleostearic acid content in oilseeds - cloning and functional characterization of triacylglycerol biosynthetic acyltransferase genes from tung (aleurites fordii) (abstract).  American Society of Plant Biology.THE ACYL-COENZYME A SYNTHETASE ENCODED BY LACS2 IS ESSENTIAL FOR NORMAL CUTICLE DEVELOPMENT IN ARABIDOPSIS.

- (Peer Reviewed Journal)Schnurr, J., Shockey, J.M., Browse, J. 2004. The acyl-coenzyme a synthetase encoded by lacs2 is essential for normal cuticle development in arabidopsis.  The Plant Cell. 16:629-642.BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ACH2, AN ACYL-COA THIOESTERASE FROM ARABIDOPSIS THALIANA.

- (Peer Reviewed Journal)Tilton, G.B., Shockey, J.M., Browse, J. 2003. Biochemical and molecular characterization of ach2, an acyl-coa thioesterase from arabidopsis thaliana. Journal of Biological Chemistry.  279:(9)7487-7494.
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