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                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research People & Locations







Eric  Cassmann
                        Virus and Prion Research

                        




                        
                        Research Veterinary Medical Officer
                        Phone: (515) 337-7190
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsElucidating the Pathobiology and Transmission of Transmissible Spongiform Encephalopathies
                                                    In-House Appropriated
                                                                    (D)Accession Number: 440677Response to APHIS SARS-CoV-2 Research Priorities Phase 1 (VPR)
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 441438Experimental Infections of Elk With SARS-CoV-2
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 442977SARS-CoV-2 White-tailed Deer Infection Dynamics and Antibody Persistence
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 443025Development of Wireless Sensors for Detecting Misfolded Prion Proteins and Rumen pH of Deer
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 442297**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Differentiation of scrapie from chronic wasting disease in white-tailed deer

- (Peer Reviewed Journal)Persistence of viral RNA in North American elk experimentally infected with an ancestral variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
                                                        

- (Peer Reviewed Journal)Boggiatto, P.M., Devries, A.C., Cassmann, E.D., Seger, H., Olsen, S.C., Palmer, M.V. 2024. Persistence of viral RNA in North American elk experimentally infected with an ancestral variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Scientific Reports. https://doi.org/10.1038/s41598-024-61414-7.The starvation-inducible Lipoprotein (Slp) plays a role in Escherichia coli O157:H7 adherence to the bovine rectoanal junction

- (Abstract Only)Temporal serum neurofilament light chain concentrations in sheep inoculated with the agent of classical scrapie
                                                        

- (Peer Reviewed Journal)Lathrop, Q.L., Nicholson, E.M., Wang, C., Greenlee, J.J., Seger, H., Veneziano, S.E., Cassmann, E.D. 2024. Temporal serum neurofilament light chain concentrations in sheep inoculated with the agent of classical scrapie. PLOS ONE. https://doi.org/10.1371/journal.pone.0299038.Update: CWD genetic resistance project at NADC

- (Trade Journal)Cassmann, E.D., Greenlee, J.J. 2023. Update: CWD genetic resistance project at NADC. North American Deer Farmer. P. 83.Short incubation periods of atypical H-BSE to cattle with EK211 and KK211 prion protein genotypes after intracranial inoculation
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Freese, L.J., Becky, K.A., Greenlee, J.J. 2023. Short incubation periods of atypical H-BSE to cattle with EK211 and KK211 prion protein genotypes after intracranial inoculation. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2023.1301998.Bovine rectoanal junction-in vitro organ culture model system to study Shiga toxin-producing Escherichia coli adherence
                                                        

- (Peer Reviewed Journal)Kudva, I.T., Biernbaum, E.N., Cassmann, E.D., Palmer, M.V. 2023. Bovine rectoanal junction-in vitro organ culture model system to study Shiga toxin-producing Escherichia coli adherence. Microorganisms. 11(5). Article 1289. https://doi.org/10.3390/microorganisms11051289.Experimental challenge of a SARS-CoV-2 Omicron variant in American mink

- (Abstract Only)The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons

- (Abstract Only)Cassmann, E.D., Qi, X., Kong, Q., Greenlee, J.J. 2023. The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons (abstract). Meeting Abstract. 4th International Chronic Wasting Disease Symposium, May 30-June 3, 2023, Denver, Colorado.Preliminary results for neurofilament light chain as a serum biomarker for chronic wasting disease in white-tailed deer

- (Abstract Only)Cassmann, E.D., Quazetta, B., Lambert, Z., Frese, L., Greenlee, J.J. 2023. Preliminary results for neurofilament light chain as a serum biomarker for chronic wasting disease in white-tailed deer (abstract). Chronic Wasting Disease Symposium Proceedings. 4th International Chronic Wasting Disease Symposium, May 30-June 3, 2023, Denver, Colorado.The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage

- (Abstract Only)Frese, A.J., Cassmann, E.D., West Greenlee, H.M., Greenlee, J.J. 2023. The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage (abstract). Meeting Abstract. 4th International Chronic Wasting Disease Symposium, May 30-June 3, 2023, Denver, Colorado.Scrapie as the potential origin of chronic wasting disease in white-tailed deer

- (Abstract Only)Lambert, Z.J., West Greenlee, H.M., Bian, J., Cassmann, E.D., Greenlee, J.J. 2023. Scrapie as the potential origin of chronic wasting disease in white-tailed deer (abstract). Chronic Wasting Disease Symposium Proceedings. 4th International Chronic Wasting Disease Symposium, May 30-June 3, 2023, Denver, Colorado.Model systems to study Shiga toxin-producing Escherichia coli interactions with the bovine gastrointestinal tract

- (Abstract Only)A mass spectrometry-based method of quantifying the contribution of the lysine polymorphism at position 171 in sheep PrP
                                                        

- (Peer Reviewed Journal)Silva, C.J., Cassmann, E.D., Greenlee, J.J., Erickson-Beltran, M.L., Requena, J.R. 2023. A mass spectrometry-based method of quantifying the contribution of the lysine polymorphism at position 171 in sheep PrP. Journal of American Society for Mass Spectrometry. 34(2):245-254. https://doi.org/10.1021/jasms.2c00277.Characterization of classical sheep scrapie in white-tailed deer after experimental oronasal exposure
                                                        

- (Peer Reviewed Journal)Greenlee, J.J., Moore, S.J., Cassmann, E.D., Lambert, Z.J., Kokemuller, R., Smith, J.D., Kunkle, R.A., Kong, Q., West Greenlee, H.M. 2022. Characterization of classical sheep scrapie in white-tailed deer after experimental oronasal exposure. Journal of Infectious Diseases. 227(12):1386-1395. Article jiac443. https://doi.org/10.1093/infdis/jiac443.Effect of inoculation with prion dilutions within the dynamic range of ELISA absorbance on prion incubation period
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Brown, Q.L., Frese, A.J., Lambert, Z.J., West Greenlee, H.M., Greenlee, J.J. 2022. Effect of inoculation with prion dilutions within the dynamic range of ELISA absorbance on prion incubation period. Veterinary Research Communications. 46(4):1377-1380. https://doi.org/10.1007/s11259-022-10013-w.Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep
                                                        

- (Peer Reviewed Journal)Harm, T.A., Smith, J.D., Cassmann, E.D., Greenlee, J.J. 2022. Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep. Research in Veterinary Science. 152:497-503. https://doi.org/10.1016/j.rvsc.2022.09.002.SARS-CoV-2: Experimental studies on deer-to-deer transmission
                                                        

- (Abstract Only)Palmer, M.V., Martins, M., Falkenberg, S.M., Devries, A.C., Caserta, L., Mitchell, P.K., Wagner, B., Cassmann, E.D., Lager, K.M., Diel, D.G., Boggiatto, P.M., Kanipe, C.R., Fernandes, M. 2022. SARS-CoV-2: Experimental studies on deer-to-deer transmission. United States Animal Health Association Proceedings. [Abstract}. https://doi.org/10.1371/journal.ppat.1010197.Comparison of protein hallmarks of ferroptosis in mice with prion disease

- (Abstract Only)Mathias, C.E., Greenlee, H.W., Greenlee, J.J., Cassmann, E.D. 2022. Comparison of protein hallmarks of ferroptosis in mice with prion disease (abstract). Meeting Abstract. p.198.Transmission of raccoon passaged chronic wasting disease agent to white-tailed deer
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Frese, A.J., Moore, S.J., Greenlee, J.J. 2022. Transmission of raccoon passaged chronic wasting disease agent to white-tailed deer. Viruses. 14(7). Article 1578. https://doi.org/10.3390/v14071578.Exploring the possibility of CWD transmission through artificial insemination of semen from CWD positive bucks

- (Trade Journal)Cassmann, E.D., Greenlee, J.J. 2022. Exploring the possibility of CWD transmission through artificial insemination of semen from CWD positive bucks. North American Deer Farmer. p. 107-109.Cattle with the EK211 PRNP polymorphism are susceptible to the H-type bovine spongiform encephalopathy agent from either E211K or wild type donors after oronasal inoculation
                                                        

- (Abstract Only)Greenlee, J.J., Cassmann, E.D., Moore, S., West Greenlee, H.M. 2022. Cattle with the EK211 PRNP polymorphism are susceptible to the H-type bovine spongiform encephalopathy agent from either E211K or wild type donors after oronasal inoculation. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):150. https://doi.org/10.1080/19336896.2022.2091286.The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons
                                                        

- (Abstract Only)Cassmann, E.D., Qi, X., Kong, Q., Greenlee, J.J. 2022. The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):120. https://doi.org/10.1080/19336896.2022.2091286.The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage
                                                        

- (Abstract Only)Frese, A.J., Cassmann, E.D., West Greenlee, H.M., Greenlee, J.J. 2022. The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):144. https://doi.org/10.1080/19336896.2022.2091286.Second passage of scrapie in white-tailed deer is discernable from chronic wasting disease
                                                        

- (Abstract Only)Lambert, Z., West Greenlee, H.M., Cassmann, E.D., Greenlee, J.J. 2022. Second passage of scrapie in white-tailed deer is discernable from chronic wasting disease. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):181. https://doi.org/10.1080/19336896.2022.2091286.From Deer-to-Deer: SARS-CoV-2 is efficiently transmitted and presents broad tissue tropism and replication sites in white-tailed deer
                                                        

- (Peer Reviewed Journal)Martins, M., Boggiatto, P.M., Buckley, A., Cassmann, E.D., Falkenberg, S.M., Caserta, L.C., Fernandes, M.H., Kanipe, C.R., Lager, K.M., Palmer, M.V., Diel, D.G. 2022. From Deer-to-Deer: SARS-CoV-2 is efficiently transmitted and presents broad tissue tropism and replication sites in highly susceptible white-tailed deer. PLoS Pathogens. 18(3). Article e1010197. https://doi.org/10.1371/journal.ppat.1010197.Chronic wasting disease isolates from Norway and North America lack evidence of common strain characteristics

- (Trade Journal)Cassmann, E.D., Greenlee, J.J. 2022. Chronic wasting disease isolates from Norway and North America lack evidence of common strain characteristics. North American Deer Farmer. p. 112.Differential accumulation of misfolded prion strains in natural hosts of prion diseases
                                                        

- (Review Article)Lambert, Z., Greenlee, J.J., Cassmann, E.D., West Greenlee, H.M. 2021. Differential accumulation of misfolded prion strains in natural hosts of prion diseases. Viruses. 13(12). Article 2453. https://doi.org/10.3390/v13122453.Why are specific tissue samples required for detection of chronic wasting disease prions

- (Trade Journal)Harm, T., Cassmann, E.D., Greenlee, J.J. 2021. Why are specific tissue samples required for detection of chronic wasting disease prions. North American Deer Farmer. p. 40-43.Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2
                                                        

- (Proceedings)Palmer, M.V., Martins, M., Falkenberg, S.M., Devries, A.C., Caserta, L.D., Mitchell, P.K., Wagner, B., Cassmann, E.D., Lager, K.M., Diel, D.G. 2021. Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2. Biocontainment Network Monthly Scientific Seminar. 95(11):e00083-21 Meeting Abstract.  https://doi.org/10.1128/JVI.00083-21.Attempted transmission of the CWD agent to deer with potentially resistant prion protein genotypes

- (Trade Journal)Greenlee, J.J., Cassmann, E.D. 2022. Attempted transmission of the CWD agent to deer with potentially resistant prion protein genotypes. North American Deer Farmer. p. 117.Experimental oronasal transmission of chronic wasting disease agent from white-tailed deer to Suffolk sheep
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Moore, S., Greenlee, J.J. 2021. Experimental oronasal transmission of chronic wasting disease agent from white-tailed deer to Suffolk sheep. Emerging Infectious Diseases. 27(12). https://doi.org/10.3201/eid2712.204978.Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2

- (Abstract Only)Palmer, M.V., Martins, M., Falkenberg, S.M., Devries, A.C., Caserta, L.D., Mitchell, P.K., Wagner, B., Cassmann, E.D., Lager, K.M., Diel, D.G. 2021. Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2. Virtual. 86th North American Wildlife and Natural Resources Conference.Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2
                                                        

- (Peer Reviewed Journal)Palmer, M.V., Martins, M., Falkenberg, S.M., Buckley, A.C., Caserta, L.D., Mitchell, P.K., Wagner, B., Cassmann, E.D., Lager, K.M., Diel, D.G. 2021. Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2. Journal of Virology. 95(11). Article e0008321. https://doi.org/10.1128/JVI.00083-21.Transmission of the atypical/nor98 scrapie agent to suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Mammadova, J., Benestad, S., Moore, S., Greenlee, J.J. 2021. Transmission of the atypical/nor98 scrapie agent to suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes. PLoS ONE. 16(2). Article e0246503. https://doi.org/10.1371/journal.pone.0246503.Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2

- (Abstract Only)Palmer, M.V., Martins, M., Falkenberg, S.M., Devries, A.C., Caserta, L.D., Mitchell, P.K., Wagner, B., Cassmann, E.D., Lager, K.M., Diel, D.G. 2020. Susceptibility of white-tailed deer (Odocoileus virginianus) to SARS-CoV-2. Virtual Symposium. December 1-3, 2020.Second passage of chronic wasting disease of mule deer in sheep compared to classical scrapie after intracranial inoculation
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Frese, R., Greenlee, J.J. 2021. Second passage of chronic wasting disease of mule deer in sheep compared to classical scrapie after intracranial inoculation. Journal of Veterinary Diagnostic Investigation. 33(4):711-720. https://doi.org/10.1177/10406387211017615.Autoclave treatment of the classical scrapie agent US No. 13-7 and experimental inoculation to susceptible VRQ/ARQ sheep via the oral route results in decreased transmission efficiency
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Mammadova, N., Greenlee, J.J. 2020. Autoclave treatment of the classical scrapie agent US No. 13-7 and experimental inoculation to susceptible VRQ/ARQ sheep via the oral route results in decreased transmission efficiency. PLoS ONE. 15(12). Article e0243009. https://doi.org/10.1371/journal.pone.0243009.Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ
                                                        

- (Peer Reviewed Journal)Cassmann, E.D., Moore, Sara, J, S.J., Kokemullar, R.D., Balkema-Buschman, A., Groschup, M., Nicholson, E.M., Greenlee, J.J. 2020. Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ. BMC Veterinary Research. 16. Article 383. https://doi.org/10.1186/s12917-020-02611-0.CWD research update from NADC

- (Trade Journal)Cassmann, E.D., Greenlee, J.J. 2020. CWD research update from NADC. North American Deer Farmer. p.72-73.
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