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                    U.S. DEPARTMENT OF AGRICULTURE
 Egg and Poultry Production Safety Research Unit: Athens, GA
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ARS Home Southeast Area Athens, Georgia U.S. National Poultry Research Center Egg and Poultry Production Safety Research Unit People & Locations







Richard K Gast
                        Egg and Poultry Production Safety Research Unit

                        




                        
                        Supervisory Microbiologist
                        Phone: (706) 546-3445
                        
                        
                        Fax: (706) 546-3035(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsReducing Pathogen Contamination Risks and Improving Quality Attributes of Eggs and Egg Products through Housing System Management and Egg Handling Practices
                                                    In-House Appropriated
                                                                    (D)Accession Number: 438593Cage-Free Pullets: Housing and Management Practices
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 439584Extensive Hen Housing: Old Idea New Tricks
                                                    Non-Assistance Cooperative Agreement
                                                                    (S)Accession Number: 445050**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Influence of egg handling and storage on the occurrence of spoilage organisms in shell eggs.

- (Abstract Only)Dust sprinkling as an effective method for infecting chickens with wild-type Salmonella Typhimurium and changes in host gut microbiota
                                                        

- (Peer Reviewed Journal)Kham, S., Mcwhorter, A., Andrews, D.M., Underwood, G.J., Moore, R.J., Gast, R.K., Chousalkar, K.K. 2024. Dust sprinkling as an effective method for infecting chickens with wild-type Salmonella Typhimurium and changes in host gut microbiota. Environmental Microbiology Reports. vol 16,(3). https://doi.org/10.1111/1758-2229.13265.A live attenuated Salmonella Typhimurium vaccine dose and diluent have minimal effects on the caecal microbiota of layer chickens.
                                                        

- (Peer Reviewed Journal)Kham, S., Mcwhorter, A., Andrews, D.M., Underwood, G.J., Moore, R.J., Gast, R.K., Chousalkar, K.K. 2024. A live attenuated Salmonella Typhimurium vaccine dose and diluent have minimal effects on the caecal microbiota of layer chickens.. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2024.1364731.Internal organ colonization by Salmonella Enteritidis in layer pullets infected at two different ages during rearing in cage-free housing

- (Peer Reviewed Journal)Cracking the code for high quality eggs: From the beginning to the end

- (Popular Publication)Jones, D.R., Gast, R.K. 2023. Cracking the code for high quality eggs: From the beginning to the end. Food Safety Magazine. https://digitaledition.food-safety.com/october-november-2023/.Internal organ colonization by Salmonella Enteritidis in layer pullets infected at two different ages during rearing in cage-free housing

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Garcia, J.S., Karcher, D.M. 2023. Internal organ colonization by Salmonella Enteritidis in layer pullets infected at two different ages during rearing in cage-free housing. Poultry Science Association Meeting Abstract. 102 (E.Suppl.1):135.Pullet Rearing Conditions and Susceptibility to Experimental Salmonella Enteritidis Infection.

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Garcia, J.S., Karcher, D.M. 2023. Pullet Rearing Conditions and Susceptibility to Experimental Salmonella Enteritidis Infection. American Association of Avian Pathologists. p.65.Impact of paddock area stocking density of free-range laying hens on egg and environmental microbiology.
                                                        

- (Peer Reviewed Journal)Garcia, J.S., Anderson, K.E., Guard, J.Y., Gast, R.K., Jones, D.R. 2023. Impact of paddock area stocking density of free-range laying hens on egg and environmental microbiology. Journal of Applied Poultry Research. https://doi.org/10.1016/j.japr.2023.100338.Internal organ colonization by salmonella enteritidis in experimentally infected layer pullets after rearing in conventional cage or cage-free housing
                                                        

- (Peer Reviewed Journal)Gast, R.K., Jones, D.R., Guraya, R., Garcia, J.S., Karcher, D.M. 2023. Internal organ colonization by salmonella enteritidis in experimentally infected layer pullets after rearing in conventional cage or cage-free housing. Journal of Applied Poultry Research. https://doi.org/10.1016/j.japr.2023.100334.Serotype screening of salmonella enterica subspecies I by intergenic sequence ribotyping (ISR): Critical updates
                                                        

- (Peer Reviewed Journal)Guard, J.Y., Jones, D.R., Gast, R.K., Garcia, J.S., Rothrock Jr, M.J. 2022. Serotype screening of salmonella enterica subspecies I by intergenic sequence ribotyping (ISR): Critical updates. Microorganisms. 11(1):97. https://doi.org/10.3390/microorganisms11010097.Understanding egg quality

- (Proceedings)Jones, D.R., Gast, R.K. 2022. Understanding egg quality. Australian Poultry Science Symposium. 33.Salmonella in eggs and egg-laying chickens
                                                        

- (Review Article)Gast, R.K., Dittoe, D.K., Ricke, S.C. 2022. Salmonella in eggs and egg-laying chickens. Critical Reviews in Microbiology. https://doi.org/10.1080/1040841X.2022.2156772.Environmental sampling methods' influence on detection of pathogens in cage-free aviary housing
                                                        

- (Peer Reviewed Journal)Garcia, J.S., Jones, D.R., Gast, R.K., Karcher, D., Erasmus, M.A. 2023. Environmental sampling methods' influence on detection of pathogens in cage-free aviary housing. Poultry Science. https://doi.org/10.1016/j.psj.2022.102381.Salmonella control in egg-laying chickens: understanding how and why

- (Proceedings)Gast, R.K. 2022. Salmonella control in egg-laying chickens: Understanding how and why. Workshop Proceedings. p. 53-57.Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared at different stocking densities in indoor cage-free housing
                                                        

- (Peer Reviewed Journal)Gast, R.K., Jones, D.R., Guraya, R., Garcia, J.S., Karcher, D.M. 2022. Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared at different stocking densities in indoor cage-free housing. Poultry Science. https://doi.org/10.1016/j.psj.2022.102104.Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared under different housing conditions

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Garcia, J.S., Karcher, D.M. 2023. Internal organ colonization by Salmonella Enteritidis in experimentally infected layer pullets reared under different housing conditions. Poultry Science Association Meeting Abstract. 101(1):157-158.Arising From: “Global spread of Salmonella Enteritidis via centralized sourcing and international trade of poultry breeding stocks” (Nature Communications 12:5109, 2021)
                                                        

- (Other)Johnson, T., Gast, R.K., Guard, J.Y., Rosales, A.G., Wilson, D. 2022. Arising From: “Global spread of Salmonella Enteritidis via centralized sourcing and international trade of poultry breeding stocks” Li, S., He, Y., Mann, D.A. et al. Global spread of Salmonella Enteritidis via centralized sourcing and international trade of poultry breeding stocks. Nat Commun 12, 5109 (2021).Tissue colonization and egg and environmental contamination associated with the experimental infection of cage-free laying hens with Salmonella Braenderup.

- (Peer Reviewed Journal)Garcia, J.S., Gast, R.K., Guard, J.Y., Karcher, D.M., Jones, D.R. 2022. Tissue colonization and egg and environmental contamination associated with the experimental infection of cage-free laying hens with Salmonella Braenderup. Avian Diseases. 66:74-78.Influence of forage substrates on environmental and egg microbiology in cage-free aviary housing flocks.
                                                        

- (Peer Reviewed Journal)Garcia, J.S., Jones, D.R., Gast, R.K., Robison, C.I., Regmi, P., Karcher, D.M. 2022. Influence of forage substrates on environmental and egg microbiology in cage-free aviary housing flocks. Journal of Applied Poultry Research. https://doi.org/10.1016/j.japr.2021.100225Contamination of eggs by Salmonella Enteritidis and Salmonella Typhimurium in experimentally infected laying hens in indoor cage-free housing.
                                                        

- (Peer Reviewed Journal)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K.E., Karcher, D.M. 2021. Contamination of eggs by Salmonella Enteritidis and Salmonella Typhimurium in experimentally infected laying hens in indoor cage-free housing. Poultry Science. 100(11). https://doi.org/10.1016/j.psj.2021.101438.Impact of organic dairy cattle manure on environmental and egg microbiology of organic free-range layer flocks.
                                                        

- (Peer Reviewed Journal)Garcia, J.S., Anderson, K., Guard, J.Y., Gast, R.K., Jones, D.R. 2021. Influence of sustainable grazing on environmental and egg microbiology of organic free-range layer flocks. Journal of Applied Poultry Research. https://doi.org/10.1016/j.japr.2021.100189.Contamination of eggs by Salmonella Enteritidis and Salmonella Typhimurium in experimentally infected laying hens in indoor cage-free housing

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K.E., Karcher, D.M. 2021. Contamination of eggs by Salmonella Enteritidis and Salmonella Typhimurium in experimentally infected laying hens in indoor cage-free housing. Poultry Science 100 (E-Suppl.1):118 (Abstr).Tissue colonization and egg and environmental contamination associated with experimental infection of laying hens with Salmonella Braenderup

- (Abstract Only)Garcia, J.S., Gast, R.K., Jones, D.R. 2021. Tissue colonization and egg and environmental contamination associated with experimental infection of laying hens with Salmonella Braenderup. Poultry Science Association Meeting Abstract. Poultry Science. 100(1):238.Salmonella control in egg-laying chickens: Understanding how and why

- (Proceedings)Gast, R.K. 2021. Salmonella control in egg-laying chickens: Understanding how and why. Proceedings of Shell Egg Academy, June 21-25, 2021. pp.9-13.Egg carton and eggshell: is there a possibility of Salmonella cross-contamination

- (Peer Reviewed Journal)Regmi, P., Jones, D.R., Gast, R.K., Guraya, R., Karcher, D. 2021. Egg carton and eggshell: is there a possibility of Salmonella cross-contamination. Applied Poultry Research. 30:100185.Equivalency of peroxyacetic acid to chlorine as a shell egg sanitizing rinse.
                                                        

- (Peer Reviewed Journal)Jones, D.R., Garcia, J.S., Gast, R.K., Ward, G.E. 2021. Equivalency of peroxyacetic acid to chlorine as a shell egg sanitizing rinse. Poultry Science. https://doi.org/10.1016/j.psj.2021.101069.Internal organ colonization and horizontal transmission of experimental Salmonella Enteritidis and Salmonella Kentucky infection in vaccinated laying hens in indoor cage-free housing.
                                                        

- (Peer Reviewed Journal)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K.E., Karcher, D.M. 2021. Internal organ colonization and horizontal transmission of experimental Salmonella Enteritidis and Salmonella Kentucky infection in vaccinated laying hens in indoor cage-free housing. Journal of Applied Poultry Research. 30:100132.Horizontal transmission and internal organ colonization by Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing.
                                                        

- (Peer Reviewed Journal)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K.E., Karcher, D.M. 2020. Horizontal transmission and internal organ colonization by Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing. Poultry Science. 99:6071-6074.Equivalency of peracetic acid to chlorine as a shell egg surface sanitizer.

- (Abstract Only)Jones, D.R., Garcia, J.S., Gast, R.K., Ward, G. 2020. Equivalency of peracetic acid to chlorine as a shell egg surface sanitizer. Poultry Science. 99(1):259.Horizontal transmission of Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing.

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K.E., Karcher, D.M. 2020. Horizontal transmission of Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing. Poultry Science Association Meeting Abstract. Poultry Science 99 (E-Suppl.1):122 (2020).Pooling of laying hen environmental swabs and efficacy of Salmonella spp. detection

- (Peer Reviewed Journal)Jones, D.R., Gast, R.K., Regmi, P., Ward, G.E., Anderson, K.E., Karcher, D.M. 2020. Pooling of laying hen environmental swabs and efficacy of Salmonella spp. detection. Journal of Food Protection. 83(6):943-950.Colonization of internal organs by Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing.

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E., Karcher, D.M. 2020. Colonization of internal organs by Salmonella Enteritidis and Salmonella Kentucky in experimentally infected laying hens in indoor cage-free housing. American Association of Avian Pathologists. Proceedings of American Association of Avian Pathologists Annual Meeting, p.10.Low dose infection of hens in lay with Salmonella enterica serovar  Enteritidis from different genomic clades
                                                        

- (Peer Reviewed Journal)Guard, J.Y., Rothrock Jr, M.J., Jones, D.R., Gast, R.K. 2020. Low dose infection of hens in lay with Salmonella enterica serovar Enteritidis from different genomic clades. Avian Diseases. 64(1):7-15. https://doi.org/10.1637/0005-2086-64.1.7.Salmonella control in egg-laying chickens

- (Proceedings)Gast, R.K. 2019. Salmonella control in egg-laying chickens. Proceedings of Shell Egg Academy. pp.20-39.Pooling of laying hen housing system environmental swabs and efficacy of Salmonella spp. detection

- (Abstract Only)Jones, D.R., Gast, R.K., Regmi, P., Ward, G.E., Anderson, K.E., Karcher, D.M. 2019. Pooling of laying hen housing system environmental swabs and efficacy of Salmonella spp. detection. Poultry Science. 98(1):205.Salmonella Enteritidis contamination of eggs from experimentally infected laying hens of four commercial genetic lines housed in conventional cages and enriched colonies.

- (Abstract Only)Gast, R.K., Regmi, P., Guraya, R., Jones, D.R., Anderson, K., Karcher, D.M. 2019. Salmonella Enteritidis contamination of eggs from experimentally infected laying hens of four commercial genetic lines housed in conventional cages and enriched colonies. Poultry Science Association Meeting Abstract. 98(1):206.Contamination of eggs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cages and enriched colony housing.

- (Peer Reviewed Journal)Gast, R.K., Regmi, P., Guraya, R., Jones, D.R., Anderson, K.E., Karcher, D.M. 2019. Contamination of eggs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cages and enriched colony housing. Poultry Science. 98:5023-5027.Salmonella Enteritidis invasion of internal organs and contamination of eggs from experimentally infected laying hens of four commercial genetic lines.

- (Abstract Only)Gast, R.K., Regmi, P., Guraya, R., Jones, D.R., Anderson, K.E., Karcher, D.M. 2019. Salmonella Enteritidis invasion of internal organs and contamination of eggs from experimentally infected laying hens of four commercial genetic lines. American Association of Avian Pathologists. p. 55.Microbiological impact of delayed movement of pullets

- (Peer Reviewed Journal)Eberle-Krish, K., Jones, D.R., Gast, R.K., Robinson, C.I., Anderson, K.E., Karcher, D.M. 2019. Microbiological impact of delayed movement of pullets. Journal of Applied Poultry Research. 20(2):318-328.Production and well-being resulting from delayed movement of pullets to the hen facility.

- (Peer Reviewed Journal)Karcher, D.M., Jones, D.R., Robinson, C.I., Eberle-Krish, K., Gast, R.K., Anderson, K. 2019. Production and well-being resulting from delayed movement of pullets to the hen facility. Journal of Applied Poultry Research. 28(2):278-289.Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cages and enriched colony housing .

- (Peer Reviewed Journal)Gast, R.K., Regmi, P., Guraya, R., Jones, D.R., Anderson, K., Karcher, D.M. 2019. Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cages and enriched colony housing.  Poultry Science. 98:1785-1790.Prevalence, persistence and antimicrobial resistance of Campylobacter spp. from eggs and laying hens housed in five commercial housing systems.

- (Peer Reviewed Journal)Novoa Rama, E., Bailey, M., Jones, D.R., Gast, R.K., Anderson, K., Brar, J., Taylor, R., Oliver, H.F., Singh, M. 2018. Prevalence, persistence and antimicrobial resistance of Campylobacter spp. from eggs and laying hens housed in five commercial housing systems. Foodborne Pathogens and Disease. 15:506-516.The impact of cage-free housing system on horizontal transmission of Salmonella in eggs

- (Abstract Only)Ward, G.E., Jones, D.R., Gast, R.K., Anderson, K., Thippareddi, H., Singh, M. 2018. The impact of cage-free housing system on horizontal transmission of Salmonella in eggs. Poultry Science Association Meeting Abstract. 97:65.Effects of different litter substrates and induced molt on production performance and welfare quality parameters of white Leghorn hens housed in multi-tiered aviary system.

- (Peer Reviewed Journal)Regmi, P., Robinson, C., Jones, D.R., Gast, R.K., Tempelman, R.J., Karcher, D.M. 2018. Effects of different litter substrates and induced molt on production performance and welfare quality parameters of white Leghorn hens housed in multi-tiered aviary system. Poultry Science. 97:3397-3404.Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cage and enriched colony housing

- (Abstract Only)Gast, R.K., Regmi, P., Guraya, R., Jones, D.R., Anderson, K., Karcher, D. 2018. Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cage and enriched colony housing. Poultry Science Association Meeting Abstract. 97: (E-Suppl.1): 112.Serotyping of Salmonella enterica isolated from mice caught on US poultry farms 1995 through 1998.

- (Peer Reviewed Journal)Guard, J.Y., Henzler, D.J., Ramadan, H., Jones, D.R., Gast, R.K., Davison, S., Allard, M.W. 2018. Serotyping of Salmonella enterica isolated from mice caught on US poultry farms 1995 through 1998. Journal of Food Safety. 6:44-50.Multiplication in egg yolk and survival in egg albumen of genetically and phenotypically characterized Salmonella Enteritidis strains

- (Peer Reviewed Journal)Gast, R.K., Guard, J.Y., Guraya, R., Locatelli, A. 2018. Multiplication in egg yolk and survival in egg albumen of genetically and phenotypically characterized Salmonella Enteritidis strains. Journal of Food Protection. 81:876-880.Salmonellosis. Diagnosis of major poultry diseases

- (Book / Chapter)Gast, R.K., Waltman, D.W. 2018. Salmonellosis. Diagnosis of major poultry diseases. Y.J. Saif and H. Toro, eds.pp. 55-59. Servet Publishing, Zaragoza, Spain.Control and monitoring of Salmonella in egg-laying chickens

- (Proceedings)Gast, R.K. 2018. Control and monitoring of Salmonella in egg-laying chickens. Proceedings of the Australian Poultry Science Symposium. 29:88-96.Salmonella infections

- (Book / Chapter)Gast, Richard K. and Robert E. Porter, Jr., 2020. Salmonella infections. Pages 719-753 in Diseases of Poultry, 14th edition. D. E. Swayne, ed. John Wiley and Sons, Hoboken, New Jersey.Review of egg-related salmonellosis and reduction strategies

- (Peer Reviewed Journal)Chousalker, K., Gast, R.K., Martelli, F., Soboleva, T., Pande, V. 2018. Review of egg-related salmonellosis and reduction strategies. Critical Reviews in Microbiology. 44:290-293.Frequency and duration of fecal shedding of Salmonella serovars Heidelberg and Typhimurium by experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Guard, J.Y., Anderson, K.E., Karcher, D.M. 2017. Frequency and duration of fecal shedding of Salmonella serovars Heidelberg and Typhimurium by experimentally infected laying hens housed in enriched colony cages at different stocking densities. Avian Diseases. 61:366-371.Frequency and duration of fecal shedding of Salmonella serovars Heidelberg and Typhimurium by experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Guard, J.Y., Anderson, K., Karcher, D.M. 2017. Frequency and duration of fecal shedding of Salmonella serovars Heidelberg and Typhimurium by experimentally infected laying hens housed in enriched colony cages at different stocking densities. Poultry Science Association Meeting Abstract.Assessing the impact of early production molting of pullets on the prevalence of Salmonella, Campylobacter, and Enterobacteriaceae

- (Abstract Only)Eberle, K.N., Jones, D.R., Gast, R.K., Robison, C.I., Anderson, K., Karcher, D. 2017. Assessing the impact of early production molting of pullets on the prevalence of Salmonella, Campylobacter, and Enterobacteriaceae. Poultry Science Association Meeting Abstract. 96:21.Evaluation of commercial cage-free barn egg quality during early production.

- (Abstract Only)Ward, G.E., Jones, D.R., Gast, R.K., Harshavardhan, T., Anderson, K. 2017. Evaluation of commercial cage-free barn egg quality during early production. Poultry Science Association Meeting Abstract. 96:26.Laying hen strain influences skeletal morphology and keel fracture prevalence in multi-tier aviary system

- (Abstract Only)Regmi, P., Jones, D.R., Gast, R.K., Karcher, D. 2017. Laying hen strain influences skeletal morphology and keel fracture prevalence in multi-tier aviary system. Poultry Science Association Meeting Abstract. 96:35.Cage-free aviary egg functionality: impact of hen strain and cold storage

- (Abstract Only)Jones, D.R., Karcher, D.M., Regmi, P., Robison, C.I., Gast, R.K. 2017. Cage-free aviary egg functionality: impact of hen strain and cold storage. Poultry Science Association Meeting Abstract. 96:89.Frequency and duration of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in enriched colony cages at different stocking densities.

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E., Karcher, D.M. 2017. Frequency and durationn of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in enriched colony cages at different stocking densities. Frontiers in Veterinary Science. 4:47.Hen genetic strain and extended cold storage implications on physical egg quality from cage-free aviary housing system.

- (Peer Reviewed Journal)Jones, D.R., Karcher, D., Regmi, P., Robison, C.O., Gast, R.K. 2018. Hen genetic strain and extended cold storage implications on physical egg quality from cage-free aviary housing system. Poultry Science. 97: 2347-2355.Foodborne Salmonella control

- (Book / Chapter)Hofacre, Charles, L., Randall Singer, and Richard K. Gast, 2017. Foodborne Salmonella control. In: A Practical Guide for Biosecurity in the Poultry Industry, 2nd edition. Jacksonville, FL. Robert L. Owen, ed., pp. 53-59.Frequency and persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K., Karcher, D.M. 2016. Frequency and persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in enriched colony cages at different stocking densities. International Poultry Scientific Forum.Sampling and detection of Salmonella in eggs

- (Book / Chapter)Gast, R.K. 2017. Sampling and detection of Salmonella in eggs. In Achieving Sustainable Production of eggs: Volume 1: Safety and Quality, J. Roberts, Ed.  Burleigh Dodds, Cambridge, UK. p.141-160.Colonization of internal organs by Salmonella serovars Heidelberg and Typhimurium in experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Guard, J.Y., Anderson, K.E., Karcher, D.M. 2017. Colonization of internal organs by Salmonella serovars Heidelberg and Typhimurium in experimentally infected laying hens housed in enriched colony cages at different stocking densities. Poultry Science. 96:1402-1409.Metabolic parameters linked by phenotype microarray to acid resistance profiles of poultry-associated Salmonella enterica.

- (Peer Reviewed Journal)Guard, J.Y., Rothrock Jr, M.J., Shah, D., Jones, D.R., Gast, R.K., Sanchez-Ingunza, R., Madsen, M., El-Attrache, J., Lungu, B. 2016. Metabolic parameters linked by phenotype microArray to acid resistance profiles of poultry-associated Salmonella enterica. Research in Microbiology. 167(9-10):745-756.Colonization of internal organs by Salmonella serovars Heidelberg and Typhimurium in experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Guard, J.Y., Anderson, K.E., Karcher, D. 2016. Colonization of internal organs by Salmonella serovars Heidelberg and Typhimurium in experimentally infected laying hens housed in enriched colony cages at different stocking densities. Poultry Science Association Meeting Abstract. Paper No. 72.Salmonella enterica.

- (Book / Chapter)Waltman, W. Douglas and Richard K. Gast, 2016. Salmonella enterica. In Isolation and Identification of Avian Pathogens, 6th edition, S. M. Williams, L. Dufour-Zavala, M. W. Jackwood, M. D. Lee, B. Lupiani,  W. M. Reed, E. Spackman, and P. R. Woolcock, eds.American Association of Avian Pathologists, Athens, GA. pp. 103-112.Microbiology of Shell Egg Production in the United States

- (Book / Chapter)Gast, R.K. 2016. Microbiology of Shell Egg Production in the United States. In Producing Safe Eggs, Steven C. Ricke and Richard K. Gast, eds. Academic Press, London, UK. p. 25-44.Colonization of internal organs by Salmonella Enteritidis in experimentally 
infected laying hens housed in enriched colony cages at different stocking densities
                                                        

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K., Karcher, D.M. 2016. Colonization of internal organs by Salmonella Enteritidis in experimentally  infected laying hens housed in enriched colony cages at different stocking densities. Poultry Science. 95:1363-1369.Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in enriched colony cages at different stocking densities

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E., Karcher, D. 2013. Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in enriched colony cages at different stocking densities. American Association of Avian Pathologist Annual Meeting. p.62.Influence of commercial laying hen housing systems on the incidence and identification of Salmonella and Campylobacter
                                                        

- (Peer Reviewed Journal)Jones, D.R., Guard, J.Y., Gast, R.K., Buhr, R.J., Fedorka-Cray, P.J., Abdo, Z., Plumblee Lawrence, J.R., Bourassa, D.V., Cox Jr, N.A., Rigsby, L.L., Robison, C.I., Regmi, P., Karcher, D.M. 2016. Influence of commercial laying hen housing systems on the incidence and identification of Salmonella and Campylobacter. Poultry Science. 95:1116-1124. https://doi.org/10.3382/ps/pew036.Microbiological Consequences of Different Housing Systems for Laying Hens: Field and Experimental Infection Studies

- (Abstract Only)Gast, R.K., Jones, D.R., Guraya, R., Anderson, K., Karcher, D.M. 2015. Microbiological Consequences of Different Housing Systems for Laying Hens: Field and Experimental Infection Studies. Proceedings of Northeast Conference on Avian Diseases. p. 10.Salmonella and impact on egg production.

- (Book / Chapter)Gast, R.K., Jones, D.R. 2017. Salmonella and impact on egg production. In Egg Production: Innovations and strategies for improvement, Patricia Hester, ed., Academic Press, Cambridge, MA. pp. 513-522.Persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in conventional or enriched cages.

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2015. Persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in conventional or enriched cages. Poultry Science Association Meeting Abstract. 94 (E-Suppl.1):60.Persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in conventional or enriched cages

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2015. Persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in conventional or enriched cages. Poultry Science. 94:1650-1656.Recovery of Salmonella enterica serovar Enteritidis from hens initially infected with serovar Kentucky

- (Peer Reviewed Journal)Guard, J.Y., Sanchez-Ingunza, R., Shah, D.H., Rothrock Jr, M.J., Gast, R.K., Jones, D.R. 2015. Recovery of Salmonella enterica serovar Enteritidis from hens initially infected with serovar Kentucky. Journal of Food Chemistry, 189:86-92. 2015.Horizontal transmission of Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2014. Horizontal transmission of Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages. Poultry Science. 93:3145-3151.Microbiological impact of three commercial laying hen housing systems

- (Peer Reviewed Journal)Jones, D.R., Cox Jr, N.A., Guard, J.Y., Cray, P.J., Buhr, R.J., Gast, R.K., Abdo, Z., Rigsby, L.L., Plumblee, J., Karcher, D.M., Robinson, C.I., Blatchford, R.A., Makagon, M.M. 2015. Microbiological impact of three commercial laying hen housing systems. Poultry Science. 94:544-551.Development and application of novel SNP-based serotyping assays in targeting Salmonella enterica within the poultry production and processing continuum.

- (Abstract Only)Horizontal transmission of Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2014. Horizontal transmission of Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages. Poultry Science Association Meeting Abstract. 93(1):263.Integrated farm management to prevent Salmonella Enteritidis contamination of eggs.

- (Peer Reviewed Journal)Trampel, D.W., Holder, T.G., Gast, R.K. 2014. Integrated farm management to prevent Salmonella Enteritidis contamination of eggs. Journal of Applied Poultry Research. 23: 353-365.Continuing multiplication of Salmonella Enteritidis strains in egg yolk during refrigeration at 7.2° C

- (Peer Reviewed Journal)Gast, R.K., Guraya, R. 2014. Continuing multiplication of Salmonella Enteritidis strains in egg yolk during refrigeration at 7.2° C. International Journal of Poultry Science. 12:622-627.Salmonella Enteritidis organ invasion and egg contamination in experimentally infected laying hens housed in conventional or enriched cages.

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2014. Salmonella Enteritidis organ invasion and egg contamination in experimentally infected laying hens housed in conventional or enriched cages. American Association of Avian Pathologist. p.20.Contamination of eggs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K. 2014. Contamination of eggs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages. Poultry Science. 93:728-733.Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages.

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2013. Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages. Poultry Science. 92:468-473.Assessing the Significance of Salmonella Heidelberg Infections in Egg-Laying Flocks

- (Proceedings)Gast, R.K. 2012. Assessing the Significance of Salmonella Heidelberg Infections in Egg-Laying Flocks. Trade Journal Publication. http://egg-cite.com/presentations/gast/slideshow.aspx.Salmonella Enteritidis deposition in eggs after experimental infection of laying hens with different oral doses

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Guard, J.Y. 2013. Salmonella Enteritidis deposition in eggs after experimental infection of laying hens with different oral doses. Journal of Food Protection. 76(1):108-113.Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hydridization for assigning serotype to Salmonella enterica.

- (Peer Reviewed Journal)Guard, J.Y., Sanchez-Ingunza, R., Morales, C., Stewart, T.E., Liljebjelke, K., Van Kessel, J.S., Ingram, K.D., Jones, D.R., Jackson, C.R., Cray, P.J., Frye, J.G., Gast, R.K., Hinton Jr, A. 2012. Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hydridization for assigning serotype to Salmonella enterica. FEMS Microbiology Letters. 337:61-72.Microbiological differences between laying hen strains housed in various production systems.

- (Abstract Only)Jones, D.R., Anderson, K., Guard, J.Y., Gast, R.K. 2012. Microbiological differences between laying hen strains housed in various production systems. Poultry Science. 91(supp):27.Salmonella Enteritidis Deposition inside Eggs after Experimental Infection of Laying Hens with Different Oral Doses

- (Abstract Only)Gast, R.K., Guraya, R., Guard, J.Y. 2012. Salmonella Enteritidis Deposition inside Eggs after Experimental Infection of Laying Hens with Different Oral Doses. Poultry Science Association. 91(Supplement 1):28.Detecting Salmonella Enteritidis in Laying Hens and Eggs after Experimental Infection at Different Oral Dose Levels

- (Abstract Only)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2012. Detecting Salmonella Enteritidis in Laying Hens and Eggs after Experimental Infection at Different Oral Dose Levels. International Association for Food Protection. 74(SupplementA):52.Controlling Egg Contamination by Understanding Salmonella Enteritidis Infections in Laying Hens

- (Proceedings)Gast, R.K., Guard, J.Y. 2012. Controlling Egg Contamination by Understanding Salmonella Enteritidis Infections in Laying Hens. World Wide Web. http://egg-cite.com/presentations/NCADC/Slideshow.aspx.Isolation of Salmonella Enteritidis from Internal Organs of Experimentally Infected Laying Hens Housed in Conventional or Enriched Cages

- (Abstract Only)Gast, R.K., Guraya, R., Jones, D.R., Anderson, K.E. 2013. Isolation of Salmonella Enteritidis from Internal Organs of Experimentally Infected Laying Hens Housed in Conventional or Enriched Cages. Poultry Science Association Meeting Abstract. 92(1):26.Salmonella Enteritidis

- (Book / Chapter)Ricke, S.C., Gast, R.K. 2014. Salmonella Enteritidis. In: Encyclopedia of Food Microbiology. Vol 3, Elsevier Ltd, Academic Press. p 343-348.CONTROLLING EGG CONTAMINATION BY UNDERSTANDING 
SALMONELLA ENTERITIDIS INFECTIONS IN LAYING HENS

- (Abstract Only)Gast, R.K., Guard, J.Y. 2012. Controlling Egg Contamination By Understanding  Salmonella Enteritidis Infections In Laying Hens.  Proceedings of North Central Avian Disease Conference. p. 49.Thermal inactivation of avian viral and bacterial pathogens in an effluent treatment system within a biosafety level 2 and 3 enhanced facility.

- (Peer Reviewed Journal)Chmielewski, R.A., Day, J.M., Spatz, S.J., Yu, Q., Gast, R.K., Zsak, L., Swayne, D.E. 2011. Thermal inactivation of avian viral and bacterial pathogens in an effluent treatment system within a biosafety level 2 and 3 enhanced facility. Applied Biosafety. 16:206-217.Salmonella Infections

- (Book / Chapter)Gast, R.K. 2013. Salmonella Infections. In Diseases of Poultry. 13th edition. D.E. Swayne, ed. Wiley-Blackwell Publishing, Ames, Iowa. p. 677.Paratyphoid Infections

- (Book / Chapter)Gast, R.K. 2013. Paratyphoid Infections. In Diseases of Poultry, 13th edition. D.E.Swayne, ed. Wiley-Blackwell Publishing, Ames, Iowa. p 693-713,718-733.Frequency and persistence of fecal shedding following exposure of laying hens to different oral doses of Salmonella enteritidis.

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Holt, P.S. 2011. Frequency and persistence of fecal shedding following exposure of laying hens to different oral doses of Salmonella enteritidi. International Journal of Poultry Science. 10:750-756.Controlling Egg Contamination with Salmonella Enteritidis by Understanding its Pathobiology

- (Proceedings)Gast, R.K., Guard, J.Y. 2011.  Controlling Egg Contamination with Salmonella Enteritidis by Understanding its Pathobiology.  PoultryMed.  Available:http://www.poultrymed.com/Poultry/Templates/ShowPage.asp?.Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella Enteritidis within phage type.

- (Peer Reviewed Journal)Guard, J.Y., Morales, C., Cray, P.J., Gast, R. K. 2011. Single nucleotide polymorphisms that differentiate two subpopulations of salmonella enteritidis within phage type. BMC Research Notes. 4:369Controlling Egg Contamination with Salmonella Enteritidis by Understanding its Pathobiology

- (Proceedings)Gast, R.K., Guard, J.Y. 2011. Controlling Egg Contamination with Salmonella Enteritidis by Understanding its Pathobiology.  In: Proceedings of the 22nd Latin American Poultry Congress, 2011.  CDROMEggs and Egg Products

- (Book / Chapter)Ricke, S., Jones, D.R., Gast, R.K. 2015. Eggs and Egg Products. In Compendium of Methods for the Microbiological Examination of Foods, 5th edition, Y. Salfinger and M.L. Tortorello, eds.,  pp. 633-643. American Public Health Association, Washington, DC.A Role for Vaccination to Control Salmonella in Poultry Flocks

- (Proceedings)Gast, R.K. 2011. A Role for Vaccination to Control Salmonella in Poultry Flocks. Electronic Publication. http://www.poultrymed.com/Poultry/Templates/showpage.asp?DBID=1&LNGID=1&TMID=111&FID=352&PID=12487.Frequency and magnitude of internal organ colonization following exposure of laying hens to different oral doses of Salmonella Enteritidis

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2011. Frequency and magnitude of internal organ colonization following exposure of laying hens to different oral doses of Salmonella Enteritidis. International Journal of Poultry Science. 10:325-331.Fecal shedding and internal organ colonization following exposure of laying hens to different oral doses of Salmonella Enteritidis

- (Abstract Only)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2011. Fecal shedding and internal organ colonization following exposure of laying hens to different oral doses of Salmonella Enteritidis. Poultry Science Symposium Proceedings. Poultry Science. (E supplement 1):2.Changes in Peyer’s Patch and cecal tonsil B lymphocytes in laying hens following challenge with Salmonella enterica serovar Enteritidis.

- (Peer Reviewed Journal)Holt, P.S., Vaughn, L., Gast, R.K. 2011. Changes in Peyer’s Patch and cecal tonsil B lymphocytes in laying hens following challenge with Salmonella enterica serovar Enteritidis. International Journal of Poultry Science. 10:231-237.A Role for Vaccination to Control Salmonella in Poultry Flocks

- (Proceedings)Gast, R.K. 2011. A Role for Vaccination to Control Salmonella in Poultry Flocks. American College of Poultry Veterinarians. Vol 1:54-59.The relationship between the numbers of Salmonella Enteritidis, Salmonella Heidelberg, or Salmonella Hadar colonizing reproductive tissues of experimentally infected laying hens and deposition inside eggs

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2011. The relationship between the numbers of Salmonella Enteritidis, Salmonella Heidelberg, or Salmonella Hadar colonizing reproductive tissues of experimentally infected laying hens and deposition inside eggs. Avian Diseases. 55:243-247.Strategies for Controlling Salmonella Enteritidis in Poultry Flocks: Translating Research into Action

- (Proceedings)Gast, R.K. 2011. Strategies for Controlling Salmonella Enteritidis in Poultry Flocks: Translating Research into Action. Meeting Proceedings. Available:http://www.bccdc.ca/NR/rdonlyres/174B83CO-E172-4663-AE01-B836436B40D7/0/Gast.pdf..The Impact of Different Housing Systems on Egg Safety and Quality

- (Proceedings)Holt, P.S., Davies, R.H., Dewulf, J., Gast, R.K., Huwe, J.K., Jones, D.R., Waltman, D., Willian, K.R. 2010. The Impact of Different Housing Systems on Egg Safety and Quality. Proceedings of Iowa Egg Symposium. Proceedings of the Iowa Egg Industry Symposium, November 10, 2010, Iowa State University, Ames, IA..Salmonella Growth and Deposition Inside Eggs: Implications for Refrigeration as a Control Strategy

- (Proceedings)Gast, R.K. 2010.  Salmonella Growth and Deposition Inside Eggs:  Implications for Refrigeration as a Control Strategy.  Georgia Poultry Conference Proceedings.  Available:  http://www.poultry.uga.edu/gaconference10.htm..Salmonella enterica serovar Enteridis (SE) infection in 5 commercial white egg and 3 commercial brown egg strains of laying hens: comparison of SE colonization with immune responsiveness

- (Abstract Only)Holt, P.S., Vaughn, L.E., Gast, R.K., Anderson, K.E. 2010. Salmonella enterica serovar Enteridis (SE) infection in 5 commercial white egg and 3 commercial brown egg strains of laying hens: comparison of SE colonization with immune responsiveness. Avian Immunology Research Group Abstract. p. 37.In vitro penetration of Salmonella Enteritidis through yolk membranes of eggs from 6 genetically distinct commercial lines of laying hens.

- (Peer Reviewed Journal)vGast, R.K., Jones, D.R., Anderson, K.E., Guraya, R., Guard, J.Y., Holt, P.S. 2010. In vitro penetration of salmonella enteritidis through yolk membranes of eggs from six genetically distinct commercial lines of laying hens. Poultry Science: 89:1732-1736.Pre-Harvest Measures to Control Salmonella in Layers

- (Book / Chapter)Gast, R.K. 2011. Pre-Harvest Measures to Control Salmonella in Layers. Improving Egg and Egg Product Safety and Quality. Cambridge, UK: Flip Van Immerseel, Yves Nys, and Maureen Bain, eds. Woodhead Publishing. (2):120-145.The impact of different housing systems on egg safety and quality.

- (Peer Reviewed Journal)iHolt, P.S., Davies, R.H., Dewulf, J., Gast, R.K., Huwe, J.K., Jones, D.R., Waltman, D., Willian, K.R. 2010. The impact of different housing systems on egg safety and quality. Poultry Science. 90:251-262.Multiplication of Salmonella Enteritidis in egg yolks after inoculation outside, on, and inside vitelline membranes and storage at different temperatures

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2010. Multiplication of Salmonella Enteritidis in egg yolks after inoculation outside, on, and inside vitelline membranes and storage at different temperatures. Journal of Food Protection. 73:1902-1906.The Impact of Different Housing Systems on Egg Safety and Quality

- (Abstract Only)Holt, P.S., Gast, R.K., Jones, D.R. 2010. The Impact of Different Housing Systems on Egg Safety and Quality. Meeting Abstract. Poultry Science. 89:831.Multiplication of Salmonella Enteritidis in egg yolks after inoculation outside, on, and inside vitelline membranes and storage at different temperatures

- (Abstract Only)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2010. Multiplication of Salmonella Enteritidis in egg yolks after inoculation outside, on, and inside vitelline membranes and storage at different temperatures. Poultry Science Association Meeting Abstract. (89): p. 856.The Relationship between the Numbers of Salmonella Enteritidis, S. Heidelberg, or S. Hadar Colonizing Reproductive Tissues of Experimentally Infected Laying Hens and Deposition inside Eggs

- (Abstract Only)Gast, R.K., Guraya, R., Guard, J.Y., Holt, P.S. 2010. The Relationship between the Numbers of Salmonella Enteritidis, S. Heidelberg, or S. Hadar Colonizing Reproductive Tissues of Experimentally Infected Laying Hens and Deposition inside Eggs. American Association of Avian Pathologists. Paper No. 9203.Selection and Characterization of Cellulose Deficient Derivatives of Shiga Toxin Producing Escherichia coli

- (Peer Reviewed Journal)Yoo, B., Stewart, T.E., Guard, J.Y., Musgrove, M.T., Gast, R.K., Chen, J. 2010. Selection and Characterization of Cellulose Deficient Mutants of Shiga Toxin Producing Escherichia coli. 2010. Applied and Environmental Microbiology. 73:1038-1046.Preharvest control of Salmonella

- (Trade Journal)Gast, R.K. 2010. Preharvest Control of Salmonella. WATT Poultry USA. p. 16-20.Comparing in vitro lymphocyte reactivity to Salmonella enterica serovar Enteridis (SE) antigens 5 commercial white egg and 3 commercial brown egg strains of laying hens following SE infection.

- (Proceedings)Holt, P.S., Vaughn, L.E., Gast, R.K., Anderson, K.E. 2010. Comparing in vitro lymphocyte reactivity to Salmonella enterica serovar Enteridis (SE) antigens 5 commercial white egg and 3 commercial brown egg strains of laying hens following SE infection. Western Poultry Disease Conference. p. 107.Salmonella Control in Laying Flocks: The Scientific Background

- (Proceedings)Gast, R.K. 2009. Salmonella Control in Laying Flocks: The Scientific Background. Proceedings of Iowa Egg Symposium: pp.1-7, 2009.Flow Cytometric Characterization of Peyer’s Patch and Cecal Tonsil T Lymphocytes in Laying Hens Following Challenge with Salmonella enterica serovar Enteritidis

- (Peer Reviewed Journal)Holt, P.S., Vaughn, L.E., Gast, R.K. 2010. Flow Cytometric Characterization of Peyer’s Patch and Cecal Tonsil T Lymphocytes in Laying Hens Following Challenge with Salmonella enterica serovar Enteritidis. Veterinary Immunology and Immunopathology. 133:276-281.The Scientific Background for Salmonella Control in Laying Flocks

- (Proceedings)Gast, R.K. 2009. The Scientific Background for Salmonella Control in Laying Flocks. Georgia Poultry Conference Proceedings.Colonization of avian reproductive tract tissues by variant subpopulations of Salmonella Enteritidis

- (Peer Reviewed Journal)Guard, J.Y., Gast, R.K., Guraya, R. 2010. Colonization of avian reproductive tract tissues by variant subpopulations of Salmonella Enteritidis. Avian Diseases. 54:857-861.Strategies for Pre-Harvest Control of Food-borne Salmonella in Poultry

- (Abstract Only)Gast, R.K. 2009. Strategies for Pre-Harvest Control of Food-borne Salmonella in Poultry. American Association of Avian Pathologists.Penetration of Salmonella enteritidis through the yolk membrane in eggs from six genetically distinct commercial lines of laying hens.

- (Abstract Only)Gast, R.K., Jones, D.R., Anderson, K.E., Guraya, R., Bouldin, J.G., Holt, P.S. 2009. Penetration of Salmonella enteritidis through the yolk membrane in eggs from six genetically distinct commercial lines of laying hens. Poultry Science Association Meeting Abstract.Effect of prior passage through laying hens on invasion of reproductive organs by Salmonella Enteritidis

- (Peer Reviewed Journal)Gast, R.K., Bouldin, J.G., Guraya, R., Holt, P.S. 2009. Effect of prior passage through laying hens on invasion of reproductive organs by Salmonella enteritidis. International Journal of Poultry Science. (8):116-121.Serologic Testing for Salmonella Infections in Poultry Flocks: Limitations and Opportunities

- (Proceedings)Gast, R.K. 2009. Serologic Testing for Salmonella Infections in Poultry Flocks: Limitations and Opportunities. American College of Poultry Veterinarians. pp.168-174.Detection of Salmonella enterica serovar Enteritidis (SE) Antibodies in Serum Using A Polystyrene Bead/SE Flagella Agglutination Assay

- (Abstract Only)Use of carboxyfluorescein diacetate succinimidyl ester (CFSE) dye and fluorescent imaging as an in situ method to visualize lymphoid tissues in egg-layer chickens challenged with Salmonella enterica serovar Enteritidis (SE)

- (Abstract Only)Mechanisms of egg contamination by Salmonella Enteritidis.

- (Peer Reviewed Journal)Gantois, I., Ducatelle, R., Pasmans, F., Haesebrouck, F., Gast, R.K., Humphrey, T., Van Immerseel, F. 2009. Mechanisms of egg contamination by Salmonella Enteritidis. FEMS Microbiology Letters. 33:718-738.Evolutionary trends of Salmonella Enteritidis linked to  subpopulation biology and virulence attributes

- (Proceedings)Guard, J.Y., Morales, C., Gast, R.K. 2008. Evolutionary trends of Salmonella Enteritidis linked to  subpopulation biology and virulence attributes. United States Animal Health Association Proceedings. 480.CELLULAR ASSESSMENT OF CROP LYMPHOID TISSUE FROM SPECIFIC-PATHOGEN-FREE WHITE LEGHORN CHICKENS AFTER SALMONELLA ENTERITIDIS CHALLENGE

- (Peer Reviewed Journal)Vaughn, L.E., Holt, P.S., Gast, R.K. 2008. Cellular assessment of crop lymphoid tissue from specific-pathogen-free white leghorn chickens after salmonella enteritidis challenge. Avian Disease. 52:657-664.In vitro multiplication of Salmonella Enteritidis on the egg yolk membrane and penetration to the yolk contents

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S. 2008. Multiplication of Salmonella Enteritidis on the yolk membrane and penetration to the yolk contents at 30 c in an in vitro egg contamination model. Journal of Food Protection. 71:1905-1909.Effect of  passage through laying hens on organ invasiveness and phenotypic heterogeneity of Salmonella enteritidis

- (Abstract Only)Gast, R.K., Bouldin, J.G., Guraya, R., Holt, P.S. 2008. Effect of  passage through laying hens on organ invasiveness and phenotypic heterogeneity of Salmonella enteritidis. Poultry Science. 87(1):54.Multiplication of Salmonella Enteritidis on Egg Yolk Membranes and Penetration into Yolk Contents

- (Abstract Only)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S. 2008. Multiplication of Salmonella Enteritidis on egg yolk membranes and penetration into yolk contents. International Association for Food Protection Proceedings, p.35.Location of Salmonella enterica serovar Enteritidis on House Flies (Musca domestica)  Obtained from Rooms Containing S. Enteritidis-challenged Hens

- (Abstract Only)Holt, P.S., Geden, C.J., Vaughn, L.E., Gast, R.K. 2008. Location of Salmonella enterica serovar Enteritidis on House Flies (Musca domestica)  Obtained from Rooms Containing S. Enteritidis-challenged Hens. American Society for Microbiology.Crop Immune Response Post-Salmonella Enteritidis Challenge in Eight Commercial Egg-Layer Strains and Specific Pathogen Free White Leghorn Chickens

- (Peer Reviewed Journal)Vaughn, L.E., Holt, P.S., Moore, R.W., Gast, R.K., Anderson, K.E. 2008. Crop Immune Response Post-Salmonella Enteritidis Challenge in Eight Commercial Egg-Layer Strains and Specific Pathogen Free White Leghorn Chickens.  Avian Disease. 52:79-87.Paratyphoid Infections

- (Book / Chapter)Gast, R.K. 2008. Paratyphoid Infections. Diseases of Poultry, 12 edition.  Blackwell Publishing, Ames, iowa, pp.636-655.Salmonella Infections

- (Book / Chapter)Gast, R.K. 2008. Salmonella Infections: Diseases of Poultry, 12th edition. Blackwell Publishing, Ames, Iowa, pp.619.Detection of Salmonella enterica subpopulations by phenotype microarray antibiotic resistance patterns

- (Peer Reviewed Journal)Bouldin, J.G., Morales, C., Frye, J.G., Gast, R.K., Musgrove, M.T. 2007. Detection of Salmonella enterica subpopulations by phenotype microarray antibiotic resistance patterns. Applied and Environmental Microbiology. 73:7753-7756.Serotype-specific and serotype-independent strategies for pre-harvest control of foodborne Salmonella in poultry.

- (Peer Reviewed Journal)Gast, R.K. 2007. Serotype-specific and serotype-independent strategies for pre-harvest control of foodborne Salmonella in poultry. Avian Diseases. 51:817-828.Isolation of Salmonella enterica serovar Enteritidis from House Flies (Musca domestica) Found in Rooms Containing Salmonella serovar Enteritidis-challenged hens.

- (Peer Reviewed Journal)Holt, P.S., Geden, C.J., Moore, R.W., Gast, R.K. 2007. Isolation of Salmonella enterica serovar Enteritidis from house flies (Musca domestica) found in rooms containing Salmonella serovar Enteritidis-challenged hens. Applied and Environmental Microbiology.73:6030-6035.Novel staining procedure to visualize the chicken Peyer’s patch in situ for expanded immunological research of this tissue in poultry

- (Abstract Only)Holt, P.S., Vaughn, L.E., Moore, R.W., Gast, R.K., Kaiser, P. 2007. Novel staining procedure to visualize the chicken Peyer’s patch in situ for expanded immunological research of this tissue in poultry. Meeting Abstract.In Vitro Penetration of Egg Yolks by Salmonella Enteritidis and Salmonella Heidelberg Strains During 36-hour Ambient Temperature Storage

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S. 2007. In Vitro Penetration of Egg Yolks by Salmonella Enteritidis and Salmonella Heidelberg Strains During 36-hour Ambient Temperature Storage. Poultry Science.86(7):1431-1435Crop Isolated Lymphoid Follicles and Ileal Peyer’s patches in Egg-Layer Hens Challenged with Salmonella enterica Enteritidis

- (Abstract Only)Holt, P.S., Vaughn, L., Moore, R.W., Gast, R.K. 2007. Crop Isolated Lymphoid Follicles and Ileal Peyer’s patches in Egg-Layer Hens Challenged with Salmonella enterica Enteritidis. American Association of Avian Pathologists.Penetration of Salmonella enteritidis and S. heidelberg strains into egg yolks during 36 hour ambient temperature storage

- (Abstract Only)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S. 2007. Penetration of Salmonella enteritidis and S. heidelberg strains into egg yolks during 36 hour ambient temperature storage. International Association for Food Protection, p 99.PATHOTYPING OF SALMONELLA ENTERICA BY ANALYSIS OF SNPS IN CYAA AND FLANKING 23S RIBOSOMAL SEQUENCES

- (Peer Reviewed Journal)Bouldin, J.G., Musgrove, M.T., Humphrey, T.J., Cates, C.W., Gast, R.K., Morales, C. 2007. Pathotyping of Salmonella enterica by analysis of SNPs in cyaA and flanking 23S ribosomal sequences. Environmental Microbiology.9:1047-1059.ARS Egg Safety and Quality Research Unit

- (Abstract Only)Gast, R.K. 2006. ARS Egg Safety and Quality Research Unit: Organization and Activities.  United States Animal Health Association Committee on Salmonella Proceedings, p 22.COLONIZATION OF SPECIFIC REGIONS OF THE REPRODUCTIVE TRACT AND DEPOSITION AT DIFFERENT LOCATIONS INSIDE EGGS LAID BY HENS INFECTED WITH SALMONELLA ENTERITIDIS OR SALMONELLA HEIDELBERG

- (Peer Reviewed Journal)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S., Moore, R.W. 2007.Colonization of specific regions of the reproductive tract and deposition at different locations inside eggs laid by hens infected with Salmonella enteritidis or Salmonella heidelberg. Avian Diseases.51:40-44.J. Linkage of avian and reproductive tract tropism with sequence divergence adjacent to the 5S ribosomal subunit rrfH of Salmonella enterica.

- (Peer Reviewed Journal)Morales, C., Gast, R.K., Bouldin, J.G. 2006. J. Linkage of avian and reproductive tract tropism with sequence divergence adjacent to the 5s ribosomal subunit rrfh of salmonella enterica.Federation of European Microbiological Societies Microbiology Letters.264:48-59. 2006.USE OF NOVEL STAINING PROCEDURE TO VISUALIZE AND ISOLATE CHICKEN PEYER’S PATCHES FOR INTESTINAL IMMUNE RESPONSE STUDIES AGAINST SALMONELLA INFECTION

- (Abstract Only)Holt, P.S., Vaughn, L.E., Moore, R.W., Gast, R.K., Kaiser, P. 2006. Use of novel staining procedure to visualize and isolate chicken peyer’s patches for intestinal immune response studies against salmonella infection. 9th Avian Immunology Group Meeting.p. 57COMPARISONS OF S ENTERICA SEROVAR ENTERITIDIS LEVELS IN CROPS OF FED OR FASTED INFECTED HENS

- (Peer Reviewed Journal)Holt, P.S., Vaughn, L.E., Moore, R.W., Gast, R.K. 2006. Comparisons of s enterica serovar enteritidis levels in crops of fed or fasted infected hens. Avian Diseases.50(3):425-429.COLONIZATION OF RERPODUCTIVE TRACT AND DEPOSITION INSIDE EGGS LAID BY HENS INFECTED WITH SALMONELLA ENTERITIDIS OR S. HEIDELBERG

- (Abstract Only)Gast, R.K., Guraya, R., Bouldin, J.G., Holt, P.S., Moore, R.W. 2006. Colonization of rerpoductive tract and deposition inside eggs laid by hens infected with salmonella enteritidis or s. heidelberg (abstract). Poultry Science Association Meeting. 85:(1):134COMPARISON OF S. ENTERICA SEROVAR ENTERITIDIS LEVELS IN CROPS OF FED OR FASTED INFECTED HENS

- (Abstract Only)Holt, P.S., Vaughn, L.E., Moore, R.W., Gast, R.K. 2007. Comparison of s. enterica serovar enteritidis levels in crops of fed or fasted infected hens. Avian Diseases: 50:425-429A NEW CRACK AT EGG SAFETY:  FROM THE HEN HOUSE TO YOUR HOUSE

- (Abstract Only)Musgrove, M.T., Curtis, P., Ramirez, G., Latimer, H., Thesmar, H., Jones, D.R., Gast, R.K. 2006. A new crack at egg safety:  from the hen house to your house (abstract).International Association for Food Protection Annual Meeting. p.67.EFFECT OF EGG REFRIGERATION ON IN VITRO PENETRATION OF SALMONELLA ENTERITIDIS THROUGH THE EGG YOLK MEMBRANE

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S., Guraya, R. 2006. Effect of egg refrigeration on in vitro penetration of salmonella enteritidis through the egg yolk membrane. Journal of Food Protection.  69:1426-1429ENHANCED GROSS VISUALIZATION OF CHICKEN PEYER’S PATCH: NOVEL STAINING TECHNIQUE APPLIED TO FRESH TISSUE SPECIMENS

- (Peer Reviewed Journal)Vaughn, L.E., Holt, P.S., Moore, R.W., Gast, R.K. 2006. Enhanced gross visualization of chicken peyer’s patch: Novel staining technique applied to fresh tissue specimens. Avaian Diseases. (50):298-302SALMONELLOSIS

- (Book / Chapter)Waltman, W.D., Gast, R.K., Mallinson, E.T. 2008. Salmonellosis. Isolation and Identification of Avian Pathogens, 5th edition.  American Association of Avian Pathologists, Jacksonville, FL, pp.3-9.HIGH EFFICIENCY ELECTROSTATIC SAMPLER FOR BIOAEROSOLS

- (Abstract Only)Mitchell, B.W., Gast, R.K. 2005. High efficiency electrostatic sampler for bioaerosols. ARS Food Safety and Inspection Service Research Workshop.DEVELOPMENT OF A LAVAGE PROCEDURE TO COLLECT LUNG SECRETIONS FROM CHICKENS FOR EVALUATING RESPIRATORY HUMORAL IMMUNITY TO SALMONELLA ENTERITIDIS

- (Peer Reviewed Journal)Holt, P.S., Stone, H.D., Moore, R.W., Gast, R.K. 2005. Development of a lavage procedure to collect lung secretions from chickens for evaluating respiratory humoral immunity to salmonella enteritidis. Avian Pathology. 34:396-398. 2005.DEVELOPMENT OF LYMPHOID TISSUES IN THE CROPS OF HENS INFECTED WITH SALMONELLA ENTERICA SUBSPECIES ENTERICA SEROVAR ENTERITIDIS

- (Abstract Only)Holt, P.S., Vaughn, L.E., Moore, R.W., Gast, R.K. 2005. Development of lymphoid tissues in the crops of hens infected with salmonella enterica subspecies enterica serovar enteritidis. International Immunology Congress.THE RELATIONSHIP BETWEEN THE DURATION OF FECAL SHEDDING AND THE PRODUCTION OF CONTAMINATED EGGS BY LAYING HENS INFECTED WITH STRAINS OF SALMONELLA ENTERITIDIS AND SALMONELLA HEIDELBERG

- (Peer Reviewed Journal)Gast, R.K., Bouldin, J.G., Holt, P.S. 2005. The relationship between the duration of fecal shedding and the production of contaminated eggs by laying hens infected with strains of salmonella enteritidis and salmonella heidelberg. Avian Diseases. Vol 49:382-386.CROP IMMUNE RESPONSE POST-SALMONELLA ENTERITIDIS CHALLENGE IN EIGHT COMMERCIAL LAYER BREED-STRAINS AND  SPECIFIC-PATHOGEN-FREE (SPF)WHITE LEGHORNS

- (Abstract Only)EFFECT OF EGG REFRIGERATION ON THE IN VITRO PENETRATION OF SALMONELLA ENTERITIDIS THROUGH THE YOLK MEMBRANE.

- (Abstract Only)Gast, R.K., Holt, P.S. 2005. Effect of egg refrigeration on the in vitro penetration of salmonella enteritidis through the yolk membrane. Poultry Science Association. 84(1):77.COMPARISON OF LEVELS OF SALMONELLA ENTERICA SEROVAR ENTERITIDIS CONTAMINATION OF FLIES OBTAINED FROM ROOMS CONTAINING MOLTED OR NONMOLTED INFECTED HENS

- (Abstract Only)Holt, P.S., Geden, C.J., Moore, R.W., Gast, R.K. 2005. Comparison of levels of salmonella enterica serovar enteritidis contamination of flies obtained from rooms containing molted or nonmolted infected hens. Western Poultry Disease Conference.THE RELATIONSHIP BETWEEN INTESTINAL PERSISTENCE AND EGG CONTAMINATION FOR STRAINS OF SALMONELLA ENTERITIDIS AND S. HEIDELBERG

- (Abstract Only)Gast, R.K., Bouldin, J.G., Holt, P.S. 2005. The relationship between intestinal persistence and egg contamination for strains of salmonella enteritidis and s. heidelberg. Program of American Association of Avian Pathologists Annual Meeting. p.43PENETRATION OF SALMONELLA ENTERITIDIS AND S. HEIDELBERG INTO EGG YOLKS IN AN IN VITRO CONTAMINATION MODEL

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S., Murase, T. 2005. Penetration of salmonella enteritidis and s. heidelberg into egg yolks in an in vitro contamination model. Poultry Science. 84:621-625GROWTH OF SALMONELLA ENTERICA SEROVAR ENTERITIDIS IN ALBUMEN AND YOLK CONTENTS OF EGGS INOCULATED WITH THIS ORGANISM ONTO THE VITELLINE MEMBRANE

- (Peer Reviewed Journal)Murase, T., Holt, P.S., Gast, R.K. 2005. Growth of salmonella enterica serovar enteritidis in albumen and yolk contents of eggs inoculated with this organism onto the vitelline membrane. Journal of Food Protection. 68(4):718-721.PENETRATION OF SALMONELLA ENTERITIDIS AND S. HEIDELBERG THROUGH THE VITELLINE MEMBRANE IN AN IN VITRO EGG CONTAMINATION MODEL

- (Abstract Only)Gast, R.K., Holt, P.S., Murase, T. 2005. Penetration of salmonella enteritidis and s. heidelberg through the vitelline membrane in an in vitro egg contamination model. Proceedings of the International Poultry Scientific Forum. 84(1):93.BACTERIAL CONTAMINATION OF EGGS

- (Book / Chapter)Gast, R.K. 2005. Bacterial contamination of eggs. In Food Safety Control in the Poultry Industry.  G.C. Mead, ed. Woodhead Publishing, Cambridge, UK, p. 1-20.HIGH EFFICIENCY ELECTROSTATIC SAMPLER FOR BIOAEROSOLS

- (Proceedings)Mitchell, B.W., Gast, R.K. 2005. High efficiency electrostatic sampler for bioaerosols. Agricultural Safety and Health American Society of Agricultural Engineers.DETECTION OF SPECIFIC ANTIBODY RESPONSES TO SALMONELLA ENTERITIDIS IN LUNG WASH SAMPLES FROM INFECTED HENS

- (Abstract Only)Holt, P.S., Stone, H.D., Gast, R.K., Moore, R.W. 2005. Detection of specific antibody responses to salmonella enteritidis in lung wash samples from infected hens. International Poultry Forum Proceedings.DEPOSITION OF SALMONELLA HEIDELBERG INSIDE EGGS IN EXPERIMENTAL INFECTION STUDIES

- (Abstract Only)Gast, R.K., Bouldin, J.G., Holt, P.S. 2004. Deposition of Salmonella Heidelberg Inside Eggs in Experimental Infection Studies. United States Animal Health Association Proceedings. p.42.Molt and Salmonella Co Infection

- (Abstract Only)Holt, P.S., Gast, R.K., Moore, R.W. 2004. Molt and Salmonella Co Infection. United States Animal Health Association Proceedings.COLONIZATION OF REPRODUCTIVE ORGANS AND INTERNAL CONTAMINATION OF EGGS AFTER EXPERIMENTAL INFECTION OF LAYING HENS WITH SALMONELLA HEIDELBERG AND SALMONELLA ENTERITIDIS

- (Peer Reviewed Journal)Gast, R.K., Bouldin, J.G., Holt, P.S. 2004. Colonization of reproductive organs and internal contamination of eggs after experimental infection of laying hens with salmonella heidelberg and salmonella enteritidis. Avian Diseases. 48:863-869PRODUCTION OF INTERNALLY CONTAMINATED EGGS BY LAYING HENS INFECTED EXPERIMENTALLY WITH SALMONELLA HEIDELBERG

- (Abstract Only)Gast, R.K., Holt, P.S., Bouldin, J.G. 2004. Production of Internally Contaminated Eggs by Laying Hens Infected Experimentally With Salmonella Heidelberg. Program of American Association of Avian Pathologists Annual Meeting. p.38.DETECTION AND CONTROL OF SALMONELLA ENTERITIDIS IN EGG LAYING CHICKENS

- (Abstract Only)Gast, R.K. 2004. Detection and Control of Salmonella Enteritidis in Egg Laying Chickens. Institute of Food Technologies Proceedings, p.235, 2004.EVALUATION OF CULTURE MEDIA FOR DETECTING AIRBORNE SALMONELLA ENTERITIDIS COLLECTED WITH AN ELECTROSTATIC SAMPLING DEVICE FROM THE ENVIRONMENT OF EXPERIMENTALLY INFECTED LAYING HENS

- (Peer Reviewed Journal)Gast, R.K., Mitchell, B.W., Holt, P.S. 2004. Evaluation of Culture Media for Detecting Airborne Salmonella Enteritidis Collected With an Electrostatic Sampling Device from the Environment of Experimentally Infected Laying Hens. Poultry Science v.83 p.1106-1111, 2004.INCUBATION OF EGG CONTENTS POOLS AT AN ELEVATED TEMPERATURE (42EC) DOES NOT IMPROVE THE RAPID DETECTION OF SALMONELLA ENTERITIDIS PHAGE TYPE 14B

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2004. Incubation of Egg Contents Pools at an Elevated temperature (42 degrees Centigrade) Does Not Improve the Rapid Detection of Salmonella Enteritidis Phage Type 14b. Journal of Food Protection. 67(8):751-1754.SUBPOPULATION BEHAVIOR OF EGG-CONTAMINATING SALMONELLA ENTERICA SEROVAR ENTERITIDIS AS DEFINED BY LIPOPOLYSACCHARIDE O CHAIN

- (Peer Reviewed Journal)Bouldin, J.G., Gast, R.K., Humphrey, T.J., Henzler, D.J., Morales, C., Coles, K. 2004. Subpopulation Behavior Of Egg-Contaminating Salmonella Enterica Serovar Enteritidis As Defined By Lipopolysaccharide O Chain. Applied and Environmental Microbiology v.70, p.2756-2763, 2004.SUBPOPULATION BEHAVIOR OF EGG-CONTAMINATING SALMONELLA ENTERICA SEROVAR ENTERITIDIS AS DEFINED BY LPS MASS

- (Proceedings)Guard, J.Y., Gast, R.K., Humphrey, T.J., Henzler, D.J., Morales, C., Coles, K. 2004. Subpopulation Behavior of Egg-Contaminating Salmonella Enterica Serovar Enteritidis as Defined by LPS Mass. 53rd Western Poultry Disease Conference. p. 27COMPARISON OF METHODS FOR DIFFERENTIATION OF SALMONELLA ENTERICA SEROVAR ENTERITIDIS PHAGE TYPE 4 ISOLATES

- (Peer Reviewed Journal)Lopes, V.C., Velayundhan, B.T., Halvorson, D.A., Lauer, D.C., Gast, R.K., Nagaraja, K.V. 2004. Comparison Of Methods For Differentiation Of Salmonella Enterica Serovar Enteritidis Phage Type 4 Isolates.  American Journal of Veterinary Research v.65 p.538-543 2004.Effect of Prior Infection with a Different Salmonella Serovar on A Salmonella Enteritidis Infection During Molt.

- (Peer Reviewed Journal)Holt, P.S., Gast, R.K. 2004. Effect of Prior Infection with a Different Salmonella Serovar on A Salmonella Enteritidis Infection During Molt. Avian Diseases. 48:160-166.DETECTION OF AIRBORNE SALMONELLA ENTERITIDIS IN THE ENVIRONMENT OF EXPERIMENTALLY INFECTED LAYING HENS BY AN ELECTROSTATIC SAMPLING DEVICE

- (Peer Reviewed Journal)Gast, R.K., Mitchell, B.W., Holt, P.S. 2004. Detection of airborne salmonella enteritidis in the environment of experimentally infected laying hens by an electrostatic sampling device. Avian Diseases.  48:148-154.PATHOGENICITY OF TWO SALMONELLA PULLORUM ISOLATES FROM BACKYARD POULTRY

- (Abstract Only)Waltman, D., Gast, R.K. 2003. Pathogenicity Of Two Salmonella Pullorum Isolates From Backyard Poultry. Proceedings of the Annual Meeting of the U.S. Animal Health Association Committee on Salmonella. p.25 2003.DETECTION OF SALMONELLA ENTERITIDIS IN THE ENVIRONMENT OF EXPERIMENTALLY INFECTED LAYING HENS BY AN ELECTROSTATIC AIR SAMPLING DEVICE

- (Abstract Only)Gast, R.K., Mitchell, B.W., Holt, P.S. 2003. Detection Of Salmonella Enteritidis In The Environment Of Experimentally Infected Laying Hens By An Electrostatic Air Sampling Device.  Proceedings of the Congress of the World Veterinary Poultry Association, p.79, 2003.EVALUATION OF CULTURE MEDIA FOR USE IN DETECTING AIRBORNE SALMONELLA ENTERITIDIS IN THE ENVIRONMENT OF EXPERIMENTALLY INFECTED LAYING HENS WITH AN ELECTROSTATIC SAMPLING DEVICE

- (Abstract Only)Gast, R.K., Mitchell, B.W., Holt, P.S. 2003. Evaluation Of Culture Media For Use In Detecting Airborne Salmonella Enteritidis In The Environment Of Experimentally Infected Laying Hens With An Electrostatic Sampling Device. United States Animal Health Association Proceedings, p.38, 2003.USE OF A LIVE ATTENUATED SALMONELLA TYPHIMURIUM VACCINE TO PROTECT HENS AGAINST S. ENTERITIDIS INFECTION WHILE UNDERGOING MOLT

- (Peer Reviewed Journal)HOLT, P.S., GAST, R.K., KELLY-AEHLE, S. USE OF A LIVE ATTENUATED SALMONELLA TYPHIMURIUM VACCINE TO PROTECT HENS AGAINST S. ENTERITIDIS INFECTION WHILE UNDERGOING MOLT. AVIAN DISEASES. 2003.   47:656-661.EFFECT OF ELEVATED INCUBATION TEMPERATURE (42 DEGREES C) ON THE MULTIPLICATION AND RAPID DETECTION OF SALMONELLA ENTERITIDIS IN EGG CONTENTS POOLS.

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2003. Effect Of Elevated Incubation Iemperature (42 Degrees C) On The Multiplication And Rapid Detection Of Salmonella Enteritidis In Egg Contents Pools. Poultry Science, 2003.V.82(Supplement 1) Abstract, p.53-54.Effect of elevated incubation temperature (42 Degrees C) On the multiplication and rapid detection of Salmonella Enteritidis in Egg Contents Pools.

- (Abstract Only)Gast, R.K., Holt, P.S. 2003. Effect of elevated incubation temperature (42 Degrees C) On the multiplication and rapid detection of Salmonella Enteritidis in Egg Contents Pools.. Poultry Science Association Meeting Abstract. p.53-54.EFFECT OF PRIOR SERIAL IN VIVO PASSAGE ON THE FREQUENCY OF SALMONELLA ENTERITIDIS CONTAMINATION IN EGGS FROM EXPERIMENTALLY INFECTED LAYING HENS

- (Peer Reviewed Journal)GAST, R.K., PETTER, J.G., HOLT, P.S. EFFECT OF PRIOR SERIAL IN VIVO PASSAGE ON THE FREQUENCY OF SALMONELLA ENTERITIDIS CONTAMINATION IN EGGS FROM EXPERIMENTALLY INFECTED LAYING HENS. AVIAN DISEASES. 2003.  47:633-639.SUBPOPULATION BEHAVIOR OF EGG-CONTAMINATING SALMONELLA ENTERICA SEROVAR ENTERITIDIS AS DEFINED BY LIPOPOLYSACCHARIDE O CHAIN

- (Proceedings)Bouldin, J.G., Gast, R.K., Humphrey, T.J., Henzler, D.J., Morales, C., Coles, K. 2003. Subpopulation behavior of egg-contaminating salmonella enterica serovar enteritidis as defined by lipopolysaccharide o chain. Western Poultry Disease Conference.SYMPOSIUM INTRODUCTION

- (Proceedings)Gast, R.K., Ricke, S.C. 2003. Symposium introduction. Poultry Science. 82:964.SIMPLE AND RAPID METHODS FOR DETECTING SALMONELLA ENTERITIDIS IN RAW EGGS

- (Peer Reviewed Journal)SEO, K., HOLT, P.S., STONE, H., GAST, R.K. SIMPLE AND RAPID METHODS FOR DETECTING SALMONELLA ENTERITIDIS IN RAW EGGS. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. 2003.  87  139-144DETECTION OF SALMONELLA ENTERITIDIS IN INCUBATED POOLS OF EGG CONTENTS BY FLUORESCENCE POLARIZATION AND LATERAL FLOW IMMUNODIFFUSION

- (Peer Reviewed Journal)GAST, R.K., HOLT, P.S., NASIR, M.S., JOLLEY, M.E., STONE, H.D. DETECTION OF SALMONELLA ENTERITIDIS IN INCUBATED POOLS OF EGG CONTENTS BY FLUORESCENCE POLARIZATION AND LATERAL FLOW IMMUNODIFFUSION. POULTRY SCIENCE. 2003.   82:687-690.INCUBATION OF SUPPLEMENTED EGG CONTENTS POOLS TO SUPPORT RAPID DETECTION OF SALMONELLA ENTERITIDIS

- (Peer Reviewed Journal)GAST, R.K., HOLT, P.S. INCUBATION OF EGG CONTENTS POOLS TO SUPPORT RAPID DETECTION OF SALMONELLA ENTERICA SEROVAR ENTERITIDIS. JOURNAL OF FOOD PROTECTION. 2003. Vol. 66. No.4. p. 656-659 2003.EFFECT OF IN VIVO PASSAGE ON THE FREQUENCY OF EGG CONTAMINATION BY SALMONELLA ENTERITIDIS IN AN EXPERIMENTAL ORAL INFECTION MODEL IN LAYING HENS

- (Abstract Only)Gast, R.K., Holt, P.S. 2002. Effect Of In Vivo Passage On The Frequency Of Egg Contamination By Salmonella Enteritidis In An Experimental Oral Infection Model in Laying Hens. United States Animal Health Association Proceedings.DETECTION OF SALMONELLA ENTERITIDIS (SE)-SPECIFIC IGA ANTIBODIES IN CROP SAMPLES FROM SE-INFECTED CHICKENS

- (Peer Reviewed Journal)HOLT, P.S., SEO, K., GAST, R.K., VAUGHN, L.E., STONE, H. DETECTION OF SALMONELLA ENTERITIDIS (SE)-SPECIFIC IGA ANTIBODIES IN CROP SAMPLES FROM SE-INFECTED CHICKENS. POULTRY SCIENCE ASSOCIATION. 2003.   82:67-70.DEVELOPMENT OF A LAVAGE PROCEDURE TO COLLECT CROP SECRETIONS FROM LIVE CHICKENS FOR STUDYING CROP IMMUNITY

- (Peer Reviewed Journal)Holt, P.S., Vaughn, L.E., Gast, R.K., Stone, H.D. 2002. Development Of A Lavage Procedure To Collect Crop Secretions From Live Chickens For Studying Crop Immunity. Avian Pathology. 31:589-592. 2002.INCUBATION OF EGG CONTENTS POOLS FOR RAPID DETECTION OF SALMONELLA ENTERITIDIS

- (Abstract Only)Gast, R.K., Holt, P.S. 2002. Incubation of Egg Contents Pools For Rapid Detection Of Salmonella Enteritidis. Poultry Science Association Meeting Abstract.INTRODUCTION: CURRENT AND FUTURE PROSPECTS FOR INDUCED MOLTING IN LAYING HENS

- (Proceedings)Gast, R.K., Ricke, S.C. 2002. Introduction: Current And Future Prospects For Induced Molting In Laying Hens. Poultry Science Meeting.DETECTION OF EXPERIMENTAL SALMONELLA ENTERITIDIS AND S. TYPHIMURIUM        INFECTIONS IN LAYING HENS BY FLUORESCENCE POLARIZATION ASSAY FOR EGG YOLK  ANTIBODIES

- (Peer Reviewed Journal)Gast, R.K., Nasir, M.S., Jolley, M.E., Holt, P.S., Stone, H.D. 2002. Detection of experimental salmonella enteritidis and s. typhimurium        infections in laying hens by fluorescence polarization assay for egg yolk  antibodies. Poultry Science.EFFECT OF PRIOR SERIAL IN VIVO PASSAGE ON THE FREQUENCY OF DEPOSITION OF SALMONELLA ENTERITIDIS IN EGGS FROM EXPERIMENTALLY INFECTED LAYING HENS

- (Abstract Only)Gast, R.K., Holt, P.S. 2002. Effect Of Prior Serial In Vivo Passage On The Frequency Of Deposition Of Salmonella Enteritidis In Eggs From Experimentally Infected Laying Hens. American Veterinary Medical Association Abstract.COMPARISON OF THE EFFECTS OF INFECTION WITH SALMONELLA ENTERITIDIS, IN     COMBINATION WITH AN INDUCED MOLT, ON SERUM LEVELS OF THE ACUTE PHASE       PROTEIN, ALPHAL ACID GLYCOPROTEIN IN HENS

- (Peer Reviewed Journal)Holt, P.S., Gast, R.K. 2002. Comparison of the effects of infection with salmonella enteritidis, in     combination with an induced molt, on serum levels of the acute phase       protein, alphal acid glycoprotein in hens. Poultry Science.CHARACTERISTICS OF SALMONELLA ENTERITIDIS CONTAMINATION IN EGGS AFTER      ORAL, AEROSOL, AND INTRAVENOUS INOCULATION OF LAYING HENS

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2002. Characteristics of salmonella enteritidis contamination in eggs after      oral, aerosol, and intravenous inoculation of laying hens. Avian Diseases.SEROLOGICAL DETECTION OF EXPERIMENTAL SALMONELLA ENTERITIDIS INFECTIONS    IN LAYING HENS BY FLUORESCENCE POLARIZATION AND ENZYME IMMUNOASSAY

- (Peer Reviewed Journal)Gast, R.K., Nasir,, M.S., Jolley,, M.E., Holt, P.S., Stone,, H.D. 2002. Serological detection of experimental salmonella enteritidis infections in laying hens by fluorescence polarization and enzyme immunoassay. Avian Diseases.DETECTING ANTIBODIES TO SALMONELLA ENTERITIDIS IN SERUM AND EGG YOLKS      FROM EXPERIMENTALLY INFECTED LAYING HENS BY FLUORESCENCE POLARIZATION

- (Abstract Only)Gast, R.K., Jolley, M.E., Nasir, M.S., Holt, P.S., Stone, H.D. 2001. Detecting antibodies to salmonella enteritidis in serum and egg yolks      from experimentally infected laying hens by fluorescence polarization. United States Animal Health Association Proceedings.INTRODUCTION AND PARATYPHOID INFECTIONS

- (Book / Chapter)COMPARISON OF SALMONELLA ENTERITIDIS INFECTION IN HENS MOLTED VIA LONG     TERM FEED WITHDRAWAL VS FULL FED WHEAT MIDDLING

- (Peer Reviewed Journal)Seo, K., Holt, P.S., Gast, R.K. 2001. Comparison of salmonella enteritidis infection in hens molted via long  term feed withdrawal vs full fed wheat middling. Journal of Food Protection.PULSED-FIELD GEL ELECTROPHORESIS AND DNA SEQUENCE ANALYSIS OF SEVERAL      SALMONELLA VIRULENCE GENES REVEALS THE CLOSE GENETIC-RELATEDNESS OF THE    MAJOR SALMONELLA ENTERITIDIS PHAGE TYPES

- (Peer Reviewed Journal)DETECTION OF EXPERIMENTAL SALMONELLA ENTERITIDIS AND S. TYPHIMURIUM        INFECTIONS IN LAYING HENS BY FLUORESCENCE POLARIZATION ASSAY FOR EGG       YOLK ANTIBODIES

- (Abstract Only)Gast, R.K., Nasir,, M.S., Jolley,, M.E., Holt, P.S., Stone,, H. 2001. Detection of experimental salmonella enteritidis and s. typhimurium        infections in laying hens by fluorescence polarization assay for egg       yolk antibodies. Poultry Science Association Meeting Abstract.THE PAST, PRESENT, AND FUTURE OF SALMONELLA CONTROL IN POULTRY: THE        EXAMPLE OF S. ENTERITIDIS

- (Proceedings)Gast, R.K. 2001. The past, present, and future of salmonella control in poultry: the example of s. enteritidis. World Veterinary Poultry Association.ASSESSING THE FREQUENCY AND CONSEQUENCES OF SALMONELLA ENTERITIDIS         DEPOSITION ON THE EGG YOLK MEMBRANE

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2001. Assessing the frequency and consequences of salmonella enteritidis deposition on the egg yolk membrane. Poultry Science.FREQUENCY AND LOCATION OF SALMONELLA ENTERITIDIS CONTAMINATION IN EGGS     ASSOCIATED WITH VARIOUS ROUTES OF EXPERIMENTAL INFECTION OF LAYING HENS

- (Abstract Only)Gast, R.K., Holt, P.S. 2001. Frequency and location of salmonella enteritidis contamination in eggs  associated with various routes of experimental infection of laying hens. American Veterinary Medical Association Abstract.DETERMINATION OF CLOSE GENETIC RELATEDNESS OF THE MAJOR SALMONELLA ENTERITIDIS PHAGE TYPES BY PULSED-FIELD GEL ELECTROPHORESIS AND DNA SEQUENCE ANALYSIS OF SEVERAL SALMONELLA VIRULENCE GENES

- (Peer Reviewed Journal)Hudson,C.R.; Garcia,M.; Gast,R.K.; Maurer,J.J., Determination of close genetic relatedness of the major salmonella enteritidis phage types by pulsed-field gel electrophoresis and DNA sequence analysis of several salmonella virulence genes. Avian Diseases issue 45 pg. 875-886THE RELATIONSHIP BETWEEN THE MAGNITUDE OF THE SPECIFIC ANTIBODY RESPONSE   TO EXPERIMENTAL SALMONELLA ENTERITIDIS INFECTION IN LAYING HENS AND        THEIR PRODUCTION OF CONTAMINATED EGGS

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2001. The relationship between the magnitude of the specific antibody response   to experimental salmonella enteritidis infection in laying hens and        their production of contaminated eggs. Avian Diseases.SEROLOGICAL DETECTION OF EXPERIMENTAL SALMONELLA ENTERITIDIS INFECTIONS IN LAYING HENS BY FLUORESCENCE POLARIZATION AND ENZYME IMMUNOASSAY

- (Abstract Only)Gast, R.K., Nasir,, M.S., Jolley,, M.E., Holt, P.S., Stone,, H.D. 2001. Serological detection of experimental salmonella enteritidis infections in laying hens by fluorescence polarization and enzyme immunoassay. Proceedings of Southern Conference on Avian Diseases.BACTERICIDAL EFFECTS OF NEGATIVE AIR IONS ON AIRBORNE AND SURFACE          SALMONELLA ENTERITIDIS FROM AN ARTIFICIALLY GENERATED AEROSOL

- (Peer Reviewed Journal)Seo, K., Mitchell, B.W., Holt, P.S., Gast, R.K. 2001. Bactericidal effects of negative air ions on airborne and surface salmonella enteritidis from an artificially generated aerosol. Journal of Food Protection.MULTIPLICATION IN EGG YOLK AND SURVIVAL IN EGG ALBUMEN OF SALMONELLA       ENTERITIDIS STRAINS OF PHAGE TYPES 4, 8, 13A AND 14B

- (Peer Reviewed Journal)Gast, R.K., Holt, P.S. 2001. Multiplication in egg yolk and survival in egg albumen of salmonella enteritidis strains of phage types 4, 8, 13a and 14b. Journal of Food Protection.ELIMINATION OF EARLY SALMONELLA ENTERITIDIS INFECTION AFTER TREATMENT WITH COMPETITIVE-EXCLUSION CULTURE AND ENROFLOXACIN IN EXPERIMENTALLY INFECTED  CHICKS

- (Peer Reviewed Journal)Seo, K., Holt, P.S., Gast, R.K., Hofacre, C.L. 2000. Elimination of early salmonella enteritidis infection after treatment with competitive-exclusion culture and enrofloxacin in experimentally infected  chicks. Poultry Science.DEPOSITION OF SALMONELLA ENTERITIDIS ON AND WITHIN EGG YOLKS

- (Proceedings)Gast, R.K., Holt, P.S. 2000. Deposition of salmonella enteritidis on and within egg yolks. Poultry Science Association Meeting Abstract.STRATEGIES AND PROGRAMS FOR CONTROLLING SALMONELLA ENTERITIDIS IN          POULTRY AND EGGS

- (Proceedings)Gast, R.K. 2000. Strategies and programs for controlling salmonella enteritidis in poultry and eggs. International Seminar on Poultry Production and Pathology Proceedings.EVALUATION OF A LATERAL FLOW DEVICE FOR DETECTING SALMONELLA ENTERITIDIS   IN RAW EGGS AND CHICKEN FECES

- (Abstract Only)Seo, K., Holt, P.S., Mitchell, B.W., Gast, R.K. 2000. Evaluation of a lateral flow device for detecting salmonella enteritidis   in raw eggs and chicken feces. Milk Food and Environmental Sanitarians International Association.THE RELATIONSHIP BETWEEN SPECIFIC ANTIBODY RESPONSE AND THE DEPOSITION OF  SALMONELLA ENTERITIDIS IN EGGS LAID BY EXPERIMENTALLY INFECTED HENS

- (Abstract Only)Gast, R.K., Holt, P.S. 2000. The relationship between specific antibody response and the deposition of  salmonella enteritidis in eggs laid by experimentally infected hens. American Veterinary Medical Association Abstract.COMPARISON OF EFFECTS OF 14 DAY FEED REMOVAL, 6 DAY FEED REMOVAL/SKIP FEED REGIMEN, AND FEEDING HENS WHEAT MIDDLINGS TO MOLT HENS ON A SALMONELLA     ENTERITIDIS INFECTION

- (Abstract Only)Holt, P.S., Seo, K., Gast, R.K. 2000. Comparison of effects of 14 day feed removal, 6 day feed removal/skip feed regimen, and feeding hens wheat middlings to molt hens on a salmonella     enteritidis infection. World Poultry Congress Proceedings.REMOVAL OF SALMONELLA ENTERITIDIS FROM ROOM ENVIRONMENTS USING             NEGATIVE AIR IONIZATION - POSSIBLE ROLE OF BACTERICIDAL ACTIVITIES BY      THE IONIZER IN THIS REMOVAL

- (Abstract Only)Seo, K., Holt, P.S., Mitchell, B.W., Gast, R.K. 2000. Removal of salmonella enteritidis from room environments using negative air ionization - possible role of bactericidal activities by      the ionizer in this removal. American Society for Microbiology.INFLUENCE OF THE LEVEL AND LOCATION OF CONTAMINATION ON THE                MULTIPLICATION OF SALMONELLA ENTERITIDIS AT DIFFERENT STORAGE              TEMPERATURES IN EXPERIMENTALLY INOCULATED EGGS

- (Peer Reviewed Journal)DEPOSITION OF PHAGE TYPE 4 AND 13A SALMONELLA ENTERITIDIS STRAINS IN THE   YOLK AND ALBUMEN OF EGGS LAID BY EXPERIMENTALLY INFECTED HENS

- (Peer Reviewed Journal)APPLICATION OF THE AGAR GEL PRECIPITIN TEST TO DETECT ANTIBODIES TO        SALMONELLA ENTERICA SEROVAR ENTERITIDIS IN SERUM AND EGG YOLKS FROM        INFECTED HENS

- (Peer Reviewed Journal)HYPORESPONSIVENESS OF THE SYSTEMIC AND MUCOSAL HUMORAL IMMUNE SYSTEMS      IN CHICKENS INFECTED WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS AT       ONE DAY OF AGE

- (Peer Reviewed Journal)EXPERIMENTAL HORIZONTAL TRANSMISSION OF SALMONELLA ENTERITIDIS STRAINS     (PHAGE TYPES 4,8, AND 13A) IN CHICKS

- (Peer Reviewed Journal)USE OF NEGATIVE AIR IONIZATION FOR REDUCING AIRBORNE LEVELS OF             SALMONELLA ENTERICA SEROVAR ENTERITIDIS IN A ROOM CONTAINING INFECTED      CAGED LAYERS

- (Peer Reviewed Journal)PERSISTENCE OF SALMONELLA ENTERITIDIS FROM ONE DAY OF AGE UNTIL MATURITY   IN EXPERIMENTALLY INFECTED EGG-TYPE CHICKENS

- (Peer Reviewed Journal)APPLICATION OF STANDARD AGGLUTINATION TESTS AND FLAGELLA-BASED             IMMUNOASSAYS FOR SEROLOGICAL DETECTION OF SALMONELLA PULLORUM              INFECTION IN CHICKENS

- (Peer Reviewed Journal)SUPPLEMENTING POOLS OF EGG CONTENTS WITH CONCENTRATED ENRICHMENT MEDIA     TO IMPROVE RAPID DETECTION OF SALMONELLA ENTERITIDIS

- (Peer Reviewed Journal)AIRBORNE HORIZONTAL TRANSMISSION OF SALMONELLA ENTERITIDIS IN MOLTED       LAYING CHICKENS

- (Peer Reviewed Journal)ASSESSING THE SENSITIVITY OF EGG YOLK ANTIBODY TESTING FOR DETECTING       SALMONELLA ENTERITIDIS INFECTIONS IN LAYING HENS

- (Peer Reviewed Journal)GROWTH OF SALMONELLA ENTERITIDIS (SE) IN EGG CONTENTS FROM HENS VACCINATED WITH AN SE BACTERIN

- (Peer Reviewed Journal)APPLYING TESTS FOR SPECIFIC YOLK ANTIBODIES TO PREDICT CONTAMINATION BY    SALMONELLA ENTERITIDIS IN EGGS FROM EXPERIMENTALLY INFECTED LAYING HENS

- (Peer Reviewed Journal)INTESTINAL COLONIZATION AND ORGAN INVASION IN CHICKS EXPERIMENTALLY        INFECTED WITH SALMONELLA ENTERITIDIS PHAGE TYPE 4 AND OTHER PHAGE TYPES    ISOLATED FROM POULTRY IN THE UNITED STATES

- (Peer Reviewed Journal)DETECTING INFECTIONS OF CHICKENS WITH RECENT SALMONELLA PULLORUM ISOLATES  USING STANDARD SEROLOGICAL METHODS

- (Peer Reviewed Journal)SALMONELLA INFECTIONS.  PARATYPHOID INFECTIONS

- (Book / Chapter)
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