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                    U.S. DEPARTMENT OF AGRICULTURE
 Foodborne Toxin Detection and Prevention Research: Albany, CA
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ARS Home Pacific West Area Albany, California Western Regional Research Center Foodborne Toxin Detection and Prevention Research People & Locations







Bradley Jay Hernlem

(Brad)
                        Foodborne Toxin Detection and Prevention Research

                        




                        
                        Chemical Engineer
                        Phone: (510) 559-5937
                        
                        
                        Fax: (510) 559-6429(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsTechnologies for the Detection of Bacterial and Plant Toxins and Allergens that Impact Food Safety and Food Defense
                                                    In-House Appropriated
                                                                    (D)Accession Number: 438572**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)A sensitive cell-based assay for measuring low-level biological activity of a-amanitin
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2023. A sensitive cell-based assay for measuring low-level biological activity of a-amanitin. International Journal of Molecular Sciences. 24(22). Article e16402. https://doi.org/10.3390/ijms242216402.Development of thermally stable nanobodies for detection and neutralization of Staphylococcal enterotoxin B
                                                        

- (Peer Reviewed Journal)Hughes, A.C., Kirkland, M., Du, W.N., Rasooly, R., Hernlem, B.J., Tam, C.C., Zhang, Y., He, X. 2023. Development of thermally stable nanobodies for detection and neutralization of Staphylococcal enterotoxin B. Toxins. 15(6). Article 400. https://doi.org/10.3390/toxins15060400.T-cell-receptor Vß8 for detection of biologically active streptococcal pyrogenic exotoxin type C
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2023. T-cell-receptor Vß8 for detection of biologically active streptococcal pyrogenic exotoxin type C. Journal of Dairy Science. 106(10):672-6730. https://doi.org/10.3168/jds.2023-23286.The effect of tannin-rich witch hazel on growth of probiotic Lactobacillus plantarum
                                                        

- (Peer Reviewed Journal)Rasooly, R., Howard, A., Balaban, N., Hernlem, B.J., Apostolidis, E. 2022. The effect of tannin-rich witch hazel on growth of probiotic Lactobacillus plantarum. Antibiotics. 11(3). Article 395. https://doi.org/10.3390/antibiotics11030395.Ex vivo and in vitro methods for detection of bioactive staphylococcal enterotoxins
                                                        

- (Book / Chapter)Rasooly, R., Do, P.M., Hernlem, B.J. 2022. Ex vivo and in vitro methods for detection of bioactive staphylococcal enterotoxins. In: Ossandon, M.R., Baker, H., Rasooly, A., editors. Biomedical Engineering Technologies. Volume 1. New York, NY: Humana Press. p. 237-255. https://doi.org/10.1007/978-1-0716-1803-5_13.Human leukemia T-cell lines as alternative to animal use for detecting biologically active Staphylococcal enterotoxin type B
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2021. Human leukemia T-cell lines as alternative to animal use for detecting biologically active Staphylococcal enterotoxin type B. Toxins. 13(5). Article 300. https://doi.org/10.3390/toxins13050300.Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2020. Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex. PLoS ONE. 15(9): Article e0238153. https://doi.org/10.1371/journal.pone.0238153.CCD based detector for detection of Abrin toxin activity
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2020. CCD based detector for detection of Abrin toxin activity. Toxins. 12(2). https://doi.org/10.3390/toxins12020120.T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2019. T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals. Toxins. 11:399. https://doi.org/10.3390/toxins11070399.Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2018. Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A. Toxins. 10(12):540. https://doi.org/10.3390/toxins10120540.Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection
                                                        

- (Peer Reviewed Journal)Rasooly, R., Magoz, Z., Luo, J., Do, P.M., Hernlem, B.J. 2018. Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection. Desalination and Water Treatment. 118:120-125. https://doi.org/10.5004/dwt.2018.22667.Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E. Sensors and Actuators B: Chemical. 259:357-393. https://doi.org/10.1016/j.snb.2017.12.079.TCR-Vß8 as alternative to animal testing for quantifying active SEE
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E. Journal of Food Protection. 80(11):1857-1862. https://doi.org/10.4315/0362-028X.JFP-17-196.Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D. Journal of Food Science. doi: 10.1111/1750-3841.13634.Generation of octoploid switchgrass by seedling treatment with mitotic inhibitors

- (Peer Reviewed Journal)Yoon, S., Aucar, S., Hernlem, B.J., Edme, S.J., Sarath, G., Blumwald, E., Tobias, C.M. 2016. Generation of octoploid switchgrass by seedling treatment with mitotic inhibitors. BioEnergy Research. doi: 10.1007/s12155-016-9795-2.Generation of Neo Octaploid Switchgrass

- (Abstract Only)Yoon, S., Blumwald, E., Mitchell, R., Gautam, S., Palmer, N., Edme, S., Hernlem, B.J., Aucar, S., Tobias, C.M. 2016. Generation of Neo Octaploid Switchgrass. Meeting Abstract. 5th Pan-American Congress on Plants and BioEnergy, Santa Fe, NM, August 4-7, 2016.Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E. Toxins. 8(5):150. doi: 10.3390/toxins8050150.Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1
                                                        

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1. Journal of Agricultural and Food Chemistry. 80:405-410.New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes
                                                        

- (Peer Reviewed Journal)Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin
                                                        

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction
                                                        

- (Peer Reviewed Journal)Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Sarreal, S.L. 2015. Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction. World Journal of Microbiology and Biotechnology. doi: 10.1007/s11274-015-1824-3..Sensitive detection of active Shiga toxin using low cost CCD based optical detector
                                                        

- (Peer Reviewed Journal)Rasooly, R., Balsam, J., Hernlem, B.J., Rasooly, A.J. 2015. Sensitive detection of active Shiga toxin using low cost CCD based optical detector. Biosensors and Bioelectronics. 68:705-711.Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells
                                                        

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876  DOI:10.3390/s140508869.Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry

- (Peer Reviewed Journal)Ravva, S.V., Hernlem, B.J., Sarreal, C.Z. 2014. Rapid Detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry. Frontiers in Cellular and Infection Microbiology. DOI:10.3389/fcimb.2014.000057.Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells
                                                        

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control

- (Abstract Only)Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Parfitt, D.E., Sarreal, S.L. 2012. Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control. American Society for Microbiology General Meeting. [abstract].Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract)

- (Abstract Only)Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria

- (Peer Reviewed Journal)Ravva, S.V., Hernlem, B.J., Sarreal, C.Z., Mandrell, R.E. 2012. Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. Journal of Environmental Monitoring. 14(2):473-481. doi: 10.1039/clem10573d.CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A

- (Peer Reviewed Journal)Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells

- (Peer Reviewed Journal)Rasooly, R., Do, P.M., Hernlem, B.J. 2011. Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells. Open Journal of Immunology. 1(1):8-14. doi:10.4236/oji.2011.11002.Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus  flavus and Pichia anomala

- (Peer Reviewed Journal)Hua, S.T., Brandl, M., Hernlem, B.J., Eng, J.G., Sarreal, S.L. 2011. Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus  flavus and Pichia anomala. Mycopathologia. 171(2):133-138.Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus

- (Abstract Only)Hua, S.T., Brandl, M., Hernlem, B.J., Sarreal, S.L. 2010. Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus. American Society for Microbiology General Meeting, San Diego, CA, May 23-27, 2010.Dihaploid stocks for switchgrass isolated by a screening approach

- (Peer Reviewed Journal)Young, H.A., Hernlem, B.J., Anderton, A.L., Lanzatella-Craig, C., Tobias, C.M. 2010. Dihaploid stocks for switchgrass isolated by a screening approach.  BioEnergy Research. 3:305-313.Dual fluorochrome flow cytometric assessment of yeast viability

- (Peer Reviewed Journal)Hernlem, B.J., Hua, S.T. 2010. Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiology. 61:57-63.Development of monoclonal antibodies specific for Ricinus agglutinins

- (Peer Reviewed Journal)Brandon, D.L., Hernlem, B.J. 2009. Development of monoclonal antibodies specific for Ricinus agglutinins. Food and Agricultural Immunology. 20(1):11-22Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples

- (Peer Reviewed Journal)Hernlem, B.J., Ravva, S.V. 2007. Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples. Journal of Environmental Monitoring. 2007(9) 1317-1322.ELECTROLYTIC DISINFECTION OF ESCHERICHIA COLI AND COLIFORM BACTERIA IN A BATCH CELL WITH DSA ELECTRODES

- (Abstract Only)Hernlem, B.J. 2006. Electrolytic disinfection of escherichia coli and coliform bacteria in a batch cell with dsa electrodes [Abstract]. ISAC XXIII International Congress. Abstract #229; Poster # P83.COMBINATION OF CHLORINE-FREE ELECTROLYTIC AND PHOTOCHEMICAL METHODS FOR STERILIZATION OF CONTAMINATED WATERS

- (Abstract Only)Barashkov, N.N., El Fenni, M., Eisenberg, D., Eisenberg, S., Lam, L., Hernlem, B.J. Combination of chlorine-free electrolytic and photochemical methods for sterilization of contaminated waters [Abstract]. The 232nd ACS National Meeting. Paper ENVR 161.ELECTROLYTIC DESTRUCTION OF UREA IN DILUTE CHLORIDE SOLUTION USING DSA ELECTRODES IN A RECYCLED BATCH CELL

- (Peer Reviewed Journal)Hernlem, B.J. 2005. "Electrolytic Destruction of Urea in Dilute Chloride Solution using DSA Electrodes in a Recycled Batch Cell." Water Research. 39(11):2245-2252ELECTROFLOTATION OF DAIRY MANURE LAGOON WATER: CHEMICAL & MICROBIOLOGICAL EFFECTS

- (Other)Hernlem, B.J. 2004. Electroflotation of dairy manure lagoon water: chemical & microbiological effects. Meeting Abstract.DISINFECTION AND SOLIDS REMOVAL OF POULTRY CHILLER WATER BY ELECTROFLOTATION

- (Peer Reviewed Journal)Tsai, L.-S., Hernlem, B.J., Huxsoll, C.C. 2002. Disinfection and Solids Removal of Poultry Chiller Water by Electroflotation. Journal of Food Science. 67(6):2160-2164.CHLORINE GENERATION AND DISINFECTION BY ELECTROFLOTATION

- (Peer Reviewed Journal)Hernlem, B.J., Tsai, L.-S. 2000. Chlorine Generation and Disinfection by Electroflotation. Journal of Food Science. 65(4):834-837.COMBINED ELECTROFLOTATION AND DISINFECTION IN FOOD PROCESSING

- (Abstract Only)Hernlem, B.J., Tsai, L.-S., Huxsoll, C., Robertson, G. 2000.  Combined Electroflotation and Disinfection in Food Processing.  Paper Number 74-12, presented at the 2000 Institute of Food Technologists Annual Meeting, June 10-14, 2000, Dallas, TX.  Available:  http://ift.confex.com/ift/2000/techprogram/paper_4502.htm.
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