U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality People & Locations







John W Schmidt
                        Meat Safety and Quality

                        




                        
                        Research Microbiologist
                        Phone: (402) 762-4226
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsIdentification, Genomic Characterization, and Metabolic Modeling of Foodborne Pathogens in the Meat Production Continuum
                                                    In-House Appropriated
                                                                    (D)Accession Number: 435565Holistic Tactics to Advance the Microbiological Safety and Quality of the Red Meat Continuum
                                                    In-House Appropriated
                                                                    (D)Accession Number: 440930Improving Detection of Antimicrobial Resistant E. coli and Salmonella in Swine
                                                    Interagency Reimbursable Agreement
                                                                    (I)Accession Number: 440538Understanding the Impact of the Farm and Lairage Environments on Salmonella Contamination in Market Hogs
                                                    Trust Fund Cooperative Agreement
                                                                    (T)Accession Number: 445120**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Antibiotic and sanitizer resistance among Escherichia coli isolated during red meat production

- (Peer Reviewed Journal)Identifying a list of Salmonella serotypes of concern to target for reducing risk of salmonellosis
                                                        

- (Peer Reviewed Journal)Katz, T.S., Harhay, D.M., Schmidt, J.W., Wheeler, T.L. 2024. Identifying a list of Salmonella serotypes of concern to target for reducing risk of salmonellosis. Frontiers in Microbiology. 15. Article 1307563. https://doi.org/10.3389/fmicb.2024.1307563.Multiplex high resolution melt curve real-time PCR assay for detection of extended-spectrum Beta-lactam-resistant Shiga toxin-producing E. coli

- (Peer Reviewed Journal)Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels

- (Abstract Only)Schmidt, J.W., Fashenpour, E., Soler-Diaz, R., Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Kang, Q., Gragg, S.E., Sanchez-Plata, M. 2023. Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels. [Abstract]. American Meat Science Association Conference Reciprocal Proceedings. No. 117.The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes

- (Abstract Only)Habib, K., Schmidt, J.W., Nichols, C.A., Kang, Q., Bosilevac, J.M., Harhay, D.M., Gragg, S.E. 2022. The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes. [Abstract]. International Association for Food Protection Proceedings. P1-83.Identification of dominant factors contributing to persistence of third-generation cephalosporin-resistant Salmonella at a beef cattle feedyard

- (Abstract Only)Schmidt, J.W., Murray, S.A., Dickey, A.M., Wheeler, T.L., Harhay, D.M., Arthur, T.M. 2023. Identification of dominant factors contributing to persistence of third-generation cephalosporin-resistant Salmonella at a beef cattle feedyard. [Abstract]. International Association for Food Protection Proceedings. T4-10.Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum
                                                        

- (Peer Reviewed Journal)Guragain, M., Schmidt, J.W., Dickey, A.M., Bosilevac, J.M. 2023. Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum. Journal of Food Protection. 86(1). Article 100031. https://doi.org/10.1016/j.jfp.2022.100031.Tetracycline-resistant, third-generation cephalosporin–resistant, and extended-spectrum b-lactamase–producing Escherichia coli in a beef cow-calf production system
                                                        

- (Peer Reviewed Journal)Agga, G.E., Galloway, H.O., Netthisinghe, A.M., Schmidt, J.W., Arthur, T.M. 2022. Tetracycline-resistant, third-generation cephalosporin–resistant, and extended-spectrum b-lactamase–producing Escherichia coli in a beef cow-calf production system. Journal of Food Protection. 85(11):1522-1530. https://doi.org/10.4315/JFP-22-178.Characterization and comparison of Salmonella spp. isolated from the mesenteric lymph nodes of cattle and the feedlot environment

- (Abstract Only)Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems
                                                        

- (Peer Reviewed Journal)Guragain, M., Schmidt, J.W., Kalchayanand, N., Dickey, A.M., Bosilevac, J.M. 2022. Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems. Nature Scientific Reports. 12. Article 5305. https://doi.org/10.1038/s41598-022-09274-x.A farm-to-fork quantitative microbial exposure assessment of beta-lactam resistant Escherichia coli among U.S. beef consumers
                                                        

- (Peer Reviewed Journal)Zhang, Y., Schmidt, J.W., Arthur, T.M., Wheeler, T.L., Zhang, Q., Wang, B. 2022. A farm-to-fork quantitative microbial exposure assessment of beta-lactam resistant Escherichia coli among U.S. beef consumers. Microorganisms. 10(3). Article 661. https://doi.org/10.3390/microorganisms10030661.Resistomes and microbiomes of meat trimmings and colon content from culled cows raised in conventional and organic production systems
                                                        

- (Peer Reviewed Journal)Weinroth, M.D., Thomas, K.M., Doster, E., Vikram, A., Schmidt, J.W., Arthur, T.M., Wheeler, T.L., Parker, J.K., Hanes, A.S., Alekoza, N., Wolfe, C., Metcalf, J.L., Morley, P.S., Belk, K.E. 2022. Resistomes and microbiomes of meat trimmings and colon content from culled cows raised in conventional and organic production systems. Animal Microbiome. 4. Article 21. https://doi.org/10.1186/s42523-022-00166-z.Twenty-four-month longitudinal study suggests little to no horizontal gene transfer in situ between third-generation cephalosporin-resistant Salmonella and third-generation cephalosporin-resistant Escherichia coli
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Murray, S.A., Dickey, A.M., Wheeler, T.L., Harhay, D.M., Arthur, T.M. 2022. Twenty-four-month longitudinal study suggests little to no horizontal gene transfer in situ between third-generation cephalosporin-resistant Salmonella and third-generation cephalosporin-resistant Escherichia coli in a beef cattle feedyard. Journal of Food Protection. 85(2):323-335. https://doi.org/10.4315/JFP-21-371.Ensuring Escherichia coli possessing colibactin genes (clb) linked to colorectal cancer do not become a food safety problem for beef

- (Abstract Only)Guragain, M., Schmidt, J.W., Kalchayanand, N., Bosilevac, J.M. 2023. Ensuring Escherichia coli possessing colibactin genes (clb) linked to colorectal cancer do not become a food safety problem for beef. Journal of Food Protection. 84(Suppl A): P. 134.Antimicrobial resistance in U.S. retail ground beef with and without label claims regarding antibiotic use
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Vikram, A., Doster, E., Thomas, K., Weinroth, M.D., Parker, J., Hanes, A., Geornaras, I., Morley, P.S., Belk, K.E., Wheeler, T.L., Arthur, T.M. 2021. Antimicrobial resistance in U.S. retail ground beef with and without label claims regarding antibiotic use. Journal of Food Protection. 84(5):827-842. https://doi.org/10.4315/JFP-20-376.A comparative quantitative assessment of human exposure to various antimicrobial-resistant bacteria among U.S. ground beef consumers
                                                        

- (Peer Reviewed Journal)Zhang, Y., Schmidt, J.W., Arthur, T.M., Wheeler, T.L., Wang, B. 2021. A comparative quantitative assessment of human exposure to various antimicrobial-resistant bacteria among U.S. ground beef consumers. Journal of Food Protection. 84(5):736-759. https://doi.org/10.4315/JFP-20-154.Metagenomic characterization of the microbiome and resistome of retail ground beef products
                                                        

- (Peer Reviewed Journal)Doster, E., Thomas, K.M., Weinroth, M.D., Parker, J.K., Crone, K.K., Arthur, T.M., Schmidt, J.W., Wheeler, T.L., Belk, K.E., Morley, P.S. 2020. Metagenomic characterization of the microbiome and resistome of retail ground beef products. Frontiers in Microbiology. 11. Article 541972.. https://doi.org/10.3389/fmicb.2020.541972.Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States
                                                        

- (Peer Reviewed Journal)Nastasijevic, I., Schmidt, J.W., Boskovic, M., Glisic, M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Bosilevac, J.M. 2020. Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States. Applied and Environmental Microbiology. 87(1):e01711-20. https://doi.org/10.1128/AEM.01711-20.Antimicrobial resistance at two United States cull cow processing establishments
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Vikram, A., Arthur, T.M., Belk, K.E., Morley, P.S., Weinroth, M.D., Wheeler, T.L. 2020. Antimicrobial resistance at two United States cull cow processing establishments. Journal of Food Protection. 83(12):2216-2228. https://doi.org/10.4315/JFP-20-201.SMART antimicrobial resistance goals to drive meat safety improvement
                                                        

- (Proceedings)Schmidt, J.W. 2020. SMART antimicrobial resistance goals to drive meat safety improvement. In: Proceedings of International Congress of Meat Science and Technology. Lake Buena Vista, Florida, Jul 31-Aug 7, 2020. Meat and Muscle Biology. 4(2):1-8. https://doi.org/10.22175/mmb.11218.No change in risk for antibiotic-resistant Salmonellosis from beef, United States, 2002-2010
                                                        

- (Peer Reviewed Journal)Costard, S., Pouzou, J., Belk, K.E., Morley, P.S., Schmidt, J.W., Wheeler, T.L., Arthur, T.M., Zagmutt, F. 2020. No change in risk for antibiotic-resistant Salmonellosis from beef, United States, 2002-2010. Emerging Infectious Diseases. 26(9):2108-2117. https://doi.org/10.3201/eid2609.190922.High prevalence of extremely heat-resistant Escherichia coli in finished beef products

- (Abstract Only)Guragain, M., Schmidt, J., Bosilevac, J.M. 2020. High prevalence of extremely heat-resistant Escherichia coli in finished beef products. [Abstract] International Association for Food Protection Proceedings, August 2-5, 2020, San Antonio, Texas [Virtual].  Journal of Food Protection. 83(Supplement A):P2-99.Persistence of third-generation cephalosporin-resistant Salmonella over two years at a beef cattle feedlot

- (Abstract Only)In-feed tylosin phosphate administration to feedlot cattle minimally affects antimicrobial resistance
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Vikram, A., Miller, E., Jones, S., Arthur, T.M. 2020. In-feed tylosin phosphate administration to feedlot cattle minimally affects antimicrobial resistance. Journal of Food Protection. 83(2):350-364. https://doi.org/10.4315/0362-028X.JFP-19-342.Cropland amendment with beef cattle manure minimally affects antimicrobial resistance
                                                        

- (Peer Reviewed Journal)Miller, E., Spiehs, M., Arthur, T.M., Woodbury, B., Cortus, E., Chatterjee, A., Rahman, S., Schmidt, J.W. 2019. Cropland amendment with beef cattle manure minimally affects antimicrobial resistance. Journal of Environmental Quality. 48:1683-1693. https://doi.org/10.2134/jeq2019.02.0042.Food service pork chops from three U.S. regions harbor similar levels of antimicrobial resistance regardless of antibiotic use claims
                                                        

- (Peer Reviewed Journal)Vikram, A., Miller, E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Schmidt, J.W. 2019. Food service pork chops from three U.S. regions harbor similar levels of antimicrobial resistance regardless of antibiotic use claims. Journal of Food Protection. 82(10):1667-1676. https://doi.org/10.4315/0362-028x.jfp-19-139.Occurrences of antimicrobial resistant bacteria and antimicrobial resistance genes in retail ground beef with and without a “Raised without Antibiotics” claim

- (Abstract Only)Antimicrobial use and resistance in United States beef production

- (Abstract Only)Schmidt, J.W., Wheeler, T.L., Arthur, T.M. 2019. Antimicrobial use and resistance in United States beef production. [Abstract]. American Chemistry Society Abstracts. Paper No. 133.Similar levels of antimicrobial resistance in U.S. food service ground beef products with and without a "Raised Without Antibiotics" claim
                                                        

- (Peer Reviewed Journal)Vikram, A., Miller, E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Schmidt, J.W. 2018. Similar levels of antimicrobial resistance in U.S. food service ground beef products with and without a "Raised Without Antibiotics" claim. Journal of Food Protection. 81(12):2007-2018. https://doi.org/10.4315/0362-028X.JFP-18-299.Effects of in-feed Chlortetracycline prophylaxis in beef cattle on antimicrobial resistance genes
                                                        

- (Peer Reviewed Journal)Miller, E.W., Vikram, A., Agga, G.E., Arthur, T.M., Schmidt, J.W. 2018. Effects of in-feed Chlortetracycline prophylaxis in beef cattle on antimicrobial resistance genes. Foodborne Pathogens and Disease. 15(1):689-697. https://doi.org/10.1089/fpd.2018.2475.A comparison of the resistome between natural and conventional retail ground beef products
                                                        

- (Abstract Only)Thomas, K., Weinroth, M., Parker, J., Metcalf, J.L., Arthur, T.M., Schmidt, J.W., Wheeler, T.L., Vikram, A., Woerner, D.R., Morley, P.S., Belk, K.E. 2019. A comparison of the resistome between natural and conventional retail ground beef products [abstract]. In: American Meat Science Association Reciprocal Meat Conference proceedings, Kansas City, MO. June 24-27, 2018. Meat and Muscle Biology. 2(2):147-148. https://doi.org/10.22175/rmc2018.130.Surface pH of fresh beef as a parameter to validate effectiveness of lactic acid treatment against Escherichia O157:H7 and Salmonella
                                                        

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Shackelford, S.D., Brown, T., Wheeler, T.L. 2018. Surface pH of fresh beef as a parameter to validate effectiveness of lactic acid treatment against Escherichia O157:H7 and Salmonella. Journal of Food Protection. 81(7):1126-1133. https://doi.org/10.4315/0362-028X.JFP-17-469.Functional blaKPC-2 sequences are present in U.S. beef cattle feces regardless of antibiotic use
                                                        

- (Peer Reviewed Journal)Vikram, A., Schmidt, J.W. 2018. Functional blaKPC-2 sequences are present in U.S. beef cattle feces regardless of antibiotic use. Foodborne Pathogens and Disease. 15(7):444-448. https://doi.org/10.1089/fpd.2017.2406.Studies performed in the proper context suggest that antimicrobial use during swine and cattle production minimally impact antimicrobial resistance
                                                        

- (Abstract Only)Schmidt, J.W., Wheeler, T.L., Arthur, T. 2018. Studies performed in the proper context suggest that antimicrobial use during swine and cattle production minimally impact antimicrobial resistance [abstract]. Journal of Animal Science. 96(Supplement 2):197. https://doi.org/10.1093/jas/sky073.362.Salmonella in peripheral lymph nodes of healthy cattle at slaughter

- (Peer Reviewed Journal)Webb, H.E., Harhay, D.M., Brashers, M.M., Nightengale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Granier, S.A., Brown, T.R., Edrington, T.S., Shackelford, S.D., Wheeler, T.L., Loneragan, G.H. 2017. Salmonella in peripheral lymph nodes of healthy cattle at slaughter. Frontiers in Microbiology. 8:2214. https://doi.org/10.3389/fmicb.2017.02214.Impact of "raised without antibiotics" beef cattle production practices on occurrences of antimicrobial resistance

- (Peer Reviewed Journal)Vikram, A., Rovira, P., Agga, G.E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Morley, P., Belk, K., Schmidt, J.W. 2017. Impact of "raised without antibiotics" beef cattle production practices on occurrences of antimicrobial resistance. Applied and Environmental Microbiology. 83:e01682-17. https://doi.org/10.1128/AEM.01682-17.Biofilm formation, antimicrobial resistance, and sanitizer tolerance of Salmonella enterica strains isolated from beef trim
                                                        

- (Peer Reviewed Journal)Wang, R., Schmidt, J.W., Harhay, D.M., Bosilevac, J.M., King, D.A., Arthur, T.M. 2017. Biofilm formation, antimicrobial resistance, and sanitizer tolerance of Salmonella enterica strains isolated from beef trim. Foodborne Pathogens and Disease. 14(12):687-695. https://doi.org/10.1089/fpd.2017.2319.Effect of winter cover crop grazing on animal performance and antibiotic resistance during pre-weaning period in beef cattle

- (Abstract Only)Agga, G.E., Galloway, H.O., Arthur, T.M., Schmidt, J.W., Netthisinghe, A.M. 2017. Effect of winter cover crop grazing on animal performance and antibiotic resistance during pre-weaning period in beef cattle. Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting. Paper No. 1327.Proper context: Comparison studies demonstrate that United States food-animal production antimicrobial uses have minimal impact on antimicrobial resistance

- (Abstract Only)Schmidt, J.W., Wheeler, T.L., Arthur, T.M. 2017. Proper context: Comparison studies demonstrate that United States food-animal production antimicrobial uses have minimal impact on antimicrobial resistance. Meeting Abstract. [Abstract]. International Sysmposium on the Environmental Dimension of Antibiotic Resistance (4th), Lansing, Michigan, August 13-17, 2017. http://www.antibiotic-resistance.de/.Providing context: antimicrobial resistance from multiple environmental sources

- (Abstract Only)Arthur, T.M., Agga, G.E., Schmidt, J.W. 2017. Providing context: antimicrobial resistance from multiple environmental sources. [Abstract]. American Society for Microbiology General Meeting. p.32, S4:8.Effect of in-feed Chlortetracycline prophylaxis in beef cattle on levels of 10 antimicrobial resistance genes

- (Abstract Only)Miller, E.W., Agga, G.E., Vikram, A., Arthur, T.M., Schmidt, J.W. 2017. Effect of in-feed Chlortetracycline prophylaxis in beef cattle on levels of 10 antimicrobial resistance genes. [Abstract]. American Society for Microbiology Meeting. p.43, no.13.Impact of raised without antibiotics practices on occurrences of antimicrobial resistance

- (Abstract Only)Vikram, A., Rovira, P., Miller, E.W., Agga, G.E., Wheeler, T.L., Arthur, T.M., Schmidt, J.W. 2017. Impact of raised without antibiotics practices on occurrences of antimicrobial resistance. [Abstract].  American Society for Microbiology General Meeting. p. 14, S1:5.Effects of in-feed Chlortetracycline Prophylaxis of beef cattle on animal health and antimicrobial-resistant Escherichia coli

- (Abstract Only)Agga, G.E., Schmidt, J.W., Arthur, T.M. 2017. Effects of in-feed Chlortetracycline Prophylaxis of beef cattle on animal health and antimicrobial-resistant Escherichia coli. Meeting Abstract. [Abstract]. Beef Industry Safety Summit, February 28-March 2, 2017, Houston, Texas, Available:http://bifsco.org/search.aspx?qal=2017 abstracts.Impact of antimicrobial use during beef production on fecal occurrence of antimicrobial resistance

- (Abstract Only)Vikram, A., Rovira, P., Agga, G.E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Morley, P., Belk, K., Schmidt, J.W. 2017. Impact of antimicrobial use during beef production on fecal occurrence of antimicrobial resistance. Meeting Abstract. [Abstract]. Beef Industry Safety Summit, February 28-March 2, 2017, Houston, Texas. Available:http://bifsco.org/search.aspx?qal=2017 abstracts.Methods for detecting pathogens in the beef food chain: detecting particular pathogens

- (Book / Chapter)Fratamico, P.M., Bosilevac, J.M., Schmidt, J.W. 2017. Methods for detecting pathogens in the beef food chain: detecting particular pathogens. In: Acuff, G., Dickson, J. Ensuring safety and quality in the production of beef. Volume 1: Safety. Cambridge, UK: Burleigh Dobbs Science. p.59-72.Effects of in-feed chlortetracycline prophylaxis of beef cattle on animal health and antimicrobial-resistant Escherichia coli
                                                        

- (Peer Reviewed Journal)Agga, G.E., Schmidt, J.W., Arthur, T.M. 2016. Effects of in-feed chlortetracycline prophylaxis of beef cattle on animal health and antimicrobial-resistant Escherichia coli. Applied and Environmental Microbiology. 82(24):7197-7204. doi:10.1128/AEM.01928-16.Methods for detecting pathogens in the beef food chain: an overview

- (Book / Chapter)Fratamico, P.M., Bosilevac, J.M., Schmidt, J.W. 2017. Methods for detecting pathogens in the beef food chain: an overview. In: Acuff, G., Dickson, J. Ensuring safety and quality in the production of beef. Volume 1: Safety. Cambridge, UK: Burleigh Dobbs Science. p.35-51.Escherichia coli O157:H7 strains isolated from High-Event Period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number
                                                        

- (Peer Reviewed Journal)Wang, R., Luedtke, B.E., Bosilevac, J.M., Schmidt, J.W., Kalchayanand, N., Arthur, T.M. 2016. Escherichia coli O157:H7 strains isolated from High Event Period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number. Journal of Food Protection. 79(11):1875-1883.  doi:10.4315/0362-028X.JFP-16-113.Effect of calcium hydroxide application to cattle feedlot pens on Escherichia coli O157:H7 and total E. coli in pen surface manure

- (Abstract Only)Berry, E.D., Wells, J., Arthur, T.M., Schmidt, J.W., Spiehs, M.J., Woodbury, B.L. 2016. Effect of calcium hydroxide application to cattle feedlot pens on Escherichia coli O157:H7 and total E. coli in pen surface manure [Abstract]. Journal of Food Protection. Supp. A(79):P2-122.Antimicrobial-resistant fecal bacteria from ceftiofur-treated and nonantimicrobial-treated comingled beef cows at a cow-calf operation
                                                        

- (Peer Reviewed Journal)Agga, G.E., Schmidt, J.W., Arthur, T.M. 2016. Antimicrobial-resistant fecal bacteria from ceftiofur-treated and nonantimicrobial-treated comingled beef cows at a cow-calf operation. Microbial Drug Resistance. 22(7):598-608. doi:10.1089/mdr.2015.0259.Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica
                                                        

- (Peer Reviewed Journal)Agga, G.E., Arthur, T.M., Schmidt, J.W., Wang, R., Harhay, D.M. 2016. Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica. Journal of Food Protection. 79(4):531-537. doi:10.4315/0362-028X.JFP-15-409.Antimicrobial resistance issues in beef production

- (Proceedings)Schmidt, J.W. 2015. Antimicrobial resistance issues in beef production.Proceedings of the American Meat Science Association Reciprocal Conference, June 13-17, 2015, Lincoln, NE, p. 55-58.Antimicrobial-resistant bacterial populations and antimicrobial resistance genes obtained from environments impacted by livestock and municipal waste
                                                        

- (Peer Reviewed Journal)Agga, G.E., Arthur, T.M., Durso, L.M., Harhay, D.M., Schmidt, J.W. 2015. Antimicrobial-resistant bacterial populations and antimicrobial resistance genes obtained from environments impacted by livestock and municipal waste. PLoS One. 10(7).Variations in the fecal occurrences of antimicrobial-resistant bacteria are greater between seasons than between "raised without antibiotics" and "conventional" cattle production systems

- (Abstract Only)Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Wheeler, T.L., Arthur, T.M. 2015. Variations in the fecal occurrences of antimicrobial-resistant bacteria are greater between seasons than between "raised without antibiotics" and "conventional" cattle production systems.[Abstract] 4th American Society for Microbiology Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens. S7:4,p.102.Antimicrobial resistance of enteric bacteria among ceftiofur treated and non-antimicrobial treated co-mingled pasture beef cows

- (Abstract Only)Agga, G.E., Schmidt, J.W., Arthur, T.M. 2015. Antimicrobial resistance of enteric bacteria among ceftiofur treated and non-antimicrobial treated co-mingled pasture beef cows.[Abstract]. 4th American Society for Microbiology Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens. S6:5, p.39.Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination

- (Abstract Only)Wang, R., Luedtke, B.E., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Arthur, T.M. 2015. Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination. [Abstract] International Association for Food Protection Proceedings. Volume 78 (Supplement A): page 64. (T10-211).Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli (STEC) and Salmonella inoculated onto fresh beef
                                                        

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2015. Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli and Salmonella inoculated onto fresh beef. Journal of Food Protection. 78(3):503-510.Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wang, R., Wheeler, T.L., Arthur, T.M. 2015. Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum. Applied and Environmental Microbiology. 81(2):713-725.Antibiotic resistant Escherichia coli and Salmonella enterica in the beef production and processing chain

- (Abstract Only)Schmidt, J.W., Harhay, D.M., Shackelford, S.D., Wheeler, T.L., Arthur, T.M. 2014. Antibiotic resistant Escherichia coli and Salmonella enterica in the beef production and processing chain. [abstract] American Society for Microbiology 114th General Meeting.  No. 1033.Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes

- (Abstract Only)Harhay, D.M., Loneragan, G.H., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Wheeler, T.L., Kolath, B., Theunick, D., Riemann, M., Schaefer, D., Brown, T. 2014. Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes. [Abstract]. International Association for Food Protection. Supp. A(77):211-212. P3-65.Examination of predictors of Salmonella enterica contamination in cattle feedlot environments

- (Abstract Only)Harhay, D.M., Arthur, T.M., Schmidt, J.W., Wang, R., Kalchayanand, N., Bosilevac, J.M., Edrington, T.S., Loneragan, G., Wheeler, T.L. 2014. Examination of predictors of Salmonella enterica contamination in cattle feedlot environments. [Abstract] Beef Industray Safety Summit. Available:  www.bifsco.org/attendees.aspx.Antibiotic resistance by source: human-cattle-swine

- (Abstract Only)Arthur, T.M., Agga, G.E., Schmidt, J.W. 2014. Antibiotic resistance by source: human-cattle-swine. [Abstract] Beef Industry Safety Summit. Available:  www.bifsco.org/attendees.aspx.Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the US

- (Abstract Only)Bosilevac, J.M., Luedtke, B.E., Arthur, T.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L. 2014. Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the U.S. [abstract] Beef Industry Safety Summit.  Available:  www.bifsco.org/attendees.aspx.Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Bosilevac, J.M., Kalchayanand, N., Wang, R., Wheeler, T.L., Koohmaraie, M. 2014. Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat. Journal of Food Protection. 77(4):538-548.Bacterial antibiotic resistance, animal agriculture, and human health: no simple answer at the interface of three complex systems

- (Proceedings)Schmidt, J.W. 2013. Bacterial antibiotic resistance, animal agriculture, and human health: no simple answer at the interface of three complex systems. Proceedings of the American Meat Science Association Reciprocal 66th Annual Meat Conference, June 16-19, 2013, Auburn, Alabama,  p.60-63.Characterization of Escherichia coli 0157:H7 strains isolated from supershedding cattle
                                                        

- (Peer Reviewed Journal)Arthur, T.M., Ahmed, R., Chase-Topping, M., Kalchayanand, N., Schmidt, J.W., Bono, J.L. 2013. Characterization of Escherichia coli 0157:H7 strains isolated from supershedding cattle. Applied and Environmental Microbiology. 79(14):4294-4303.Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances
                                                        

- (Peer Reviewed Journal)Wang, R., Kalchayanand, N., Schmidt, J.W., Harhay, D.M. 2013. Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances. Journal of Food Protection. 76(9):1513-1522.Longitudinal concentrations of antibiotic resistant Escherichia coli in feces do not correspond to the patterns of antibiotic use at a cattle feedlot

- (Abstract Only)Schmidt, J.W. 2013. Longitudinal concentrations of antibiotic resistant Escherichia coli in feces do not correspond to the patterns of antibiotic use at a cattle feedlot. American Society for Microbiology Annual Meeting. Poster No. 15 p. 101.Characterization of E. coli O157:H7 strains isolated from super-shedding cattle

- (Abstract Only)Arthur, T.M., Ahmed, R., Chase-Topping, M., Kalchayanand, N., Schmidt, J.W., Bono, J.L. 2013. Characterization of E. coli O157:H7 strains isolated from super-shedding cattle. American Society for Microbiology General Meeting. Poster No. 1707, p 171.Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest

- (Peer Reviewed Journal)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.Salmonella in lymph nodes of cull and fed cattle at harvest

- (Abstract Only)Webb, H.E., Harhay, D.M., Gragg, S.E., Brashears, M.M., Nightingale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Lonergan, G.H. 2013. Salmonella in lymph nodes of cull and fed cattle at harvest. IN:  Proceedings of The Beef Industry Summit 2013, March 13-15, 2013, Dallas Texas, 2013 FLASHDRIVE.Influence of therapeutic ceftiofur treatments of feedlot cattle on fecal and hide prevalences of commensal Escherichia coli resistant to expanded-spectrum cephalosporins, and molecular characterization of resistant isolates
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Griffin, D., Kuehn, L.A., Harhay, D.M. 2013. Influence of therapeutic ceftiofur treatments of feedlot cattle on fecal and hide prevalences of commensal Escherichia coli resistant to expanded-spectrum cephalosporins, and molecular characterization of resistant isolates. Applied and Environmental Microbiology. 79(7):2273-2283.Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest

- (Peer Reviewed Journal)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10(4):368-374.The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests

- (Peer Reviewed Journal)Wang, R., Schmidt, J.W., Arthur, T.M., Bosilevac, J.M. 2013. The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests. Food Microbiology. 33: 205-212.Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three U.S. commercial beef processing plants

- (Peer Reviewed Journal)Schmidt, J.W., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L. 2012. Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three U. S. commercial beef processing plants. Journal of Food Protection. 75(12):2213-2218.Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains

- (Peer Reviewed Journal)SWang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.Prevalence of lactose fermenting coliforms resistant to third generation cephalosporins in cattle feedlot throughout a production cycle and molecular characterization of resistant isolates

- (Abstract Only)Schmidt, J.W., Kuehn, L.A., Griffin, D., Harhay, D.M. 2012. Prevalence of lactose fermenting coliforms resistant to third generation cephalosporins in cattle feedlot throughout a production cycle and molecular characterization of resistant isolates [abstract]. International Association for Food Protection. p. 148-149. Abstract No. P2-95.Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2012. Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. Journal of Food Protection. 75(7):1207-1212.Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants

- (Peer Reviewed Journal)Schmidt, J.W., Harhay, D.M., Kalchayanand, N., Bosilevac, J.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2012. Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants. Applied and Environmental Microbiology. 78(8):2716-2726.Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention

- (Peer Reviewed Journal)Schmidt, J.W., Wang, R., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2012. Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention. Journal of Food Protection. 75 (5): 955-958.Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica

- (Peer Reviewed Journal)Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Loneragan, G.H., Wheeler, T.L. 2012. Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica. Journal of Food Protection. 75(5):854-858.Salmonella in lymph nodes of cattle presented for harvest

- (Abstract Only)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois.  p. 149.Salmonella in lymph nodes of cattle presented for harvest

- (Abstract Only)Gragg, S.E., Loneragan, G.H., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brashears, M.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. International Association for Food Protection.  p. 19.  Abstract No. P1-62.Towards an understanding of how Salmonella potentially evades beef carcass processing interventions

- (Abstract Only)Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7

- (Abstract Only)Bosilevac, J.M., Schmidt, J.W., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2011. The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7 [abstract]. 2011 Beef Industry Safety Summit, Dallas, TX. March 2-4, 2011. 2011 Beef Industry Safety Summit Research Update. Abstract No. 1.Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods

- (Peer Reviewed Journal)Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods. Journal of Food Protection. 73:2180-2188.
                    ARS News Articles
                            item
                                                Scientists Scrutinize Role of "Supershedder" Cattle in E. coli O157:H7 Contamination


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov