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 Plant, Soil and Nutrition Research: Ithaca, NY
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ARS Home Northeast Area Ithaca, New York Robert W. Holley Center for Agriculture & Health Plant, Soil and Nutrition Research People & Locations







Miguel A Pineros
                        Plant, Soil and Nutrition Research

                        




                        
                        Research Plant Physiologist
                        Phone: (607) 255-5116
                        
                        
                        Fax: (607) 255-1132(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsFunction, Structure and Regulation of Membrane Transport Proteins Mediating Plant Mineral Nutrition and Abiotic Stress Responses
                                                    Cooperative Agreement
                                                                    (A)Accession Number: 441075Biochemistry and Physiology of Crop Adaptation to Soil-Based Abiotic Stresses
                                                    In-House Appropriated
                                                                    (D)Accession Number: 443622Elucidating the Grape Malate Tonoplast Transporter: A Targeted Approach for Controlling Fruit & Wine Acidity & Improving Grapevine Tolerance
                                                    Reimbursable Cooperative Agreement
                                                                    (R)Accession Number: 445430**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)A single amino acid substitution in MdLAZY1A dominantly impairs shoot gravitropism in Malus
                                                        

- (Peer Reviewed Journal)Doughtery, L., Borejsza-Wysocka, E., Miaule, A., Wang, P., Zheng, D., Jansen, M., Brown, S., Pineros, M., Dardick, C., Xu, K., Monaghan, J., Chabot, D., Subramaniam, R., Blackwell, B., Harris, L. 2023. A single amino acid substitution in MdLAZY1A dominantly impairs shoot gravitropism in Malus. Plant Physiology. https://doi.org/10.1093/plphys/kiad373.Loss of OPT3 function decreases phloem copper levels and impairs crosstalk between copper and iron  homeostasis and shoot-to-root signaling in Arabidopsis thaliana
                                                        

- (Peer Reviewed Journal)Chia, J., Yan, J., Ishka, M.R., Faulkner, M.M., Huang, R., Smieska, L., Woll, A., Tappero, R., Kiss, A., Jiao, C., Fei, Z., Pineros, M., Kochian, L., Walker, E., Vatamaniuk, O.K. 2023. Loss of OPT3 function decreases phloem copper levels and impairs crosstalk between copper and iron  homeostasis and shoot-to-root signaling in Arabidopsis thaliana. The Plant Cell. 35(6):2157-2185. https://doi.org/10.1093/plcell/koad053.Identifying genes associated with abiotic stress tolerance suitable for CRISPR/Cas9 editing in upland rice cultivars adapted to acid soils
                                                        

- (Peer Reviewed Journal)Barrero, L., William, M., Craft, E.J., Akther, K., Martinez, G., Glahn, R.P., Harrington, S., Pineros, M., Mccouch, S. 2022. Identifying genes associated with abiotic stress tolerance suitable for CRISPR/Cas9 editing in upland rice cultivars adapted to acid soils. Plant Direct. 6(12):Article e469. https://doi.org/10.1002/pld3.469.Al-induced and -activated signals in aluminium resistance
                                                        

- (Peer Reviewed Journal)Koyama, H., Huang, C., Pineros, M., Yamamoto, Y. 2022. Al-induced and -activated signals in aluminium resistance. Frontiers in Plant Science. 13. Article e925541. https://doi.org/10.3389/fpls.2022.925541.Cell-free synthesis of a transmembrane mechanosensitive channel protein into a hybrid-supported lpid bilayer
                                                        

- (Peer Reviewed Journal)Manzer, Z., Ghosh, S., Jacobs, M.L., Krishnan, S., Zipfel, W.R., Pineros, M., Kamat, N.P., Daniel, S. 2021. Cell-free synthesis of a transmembrane mechanosensitive channel protein into a hybrid-supported lpid bilayer. ACS Applied Materials and Interfaces. 4(4):3101-3112. https://doi.org/10.1021/acsabm.0c01482.Plant HKT Channels: an updated view on structure, function and gene regulation
                                                        

- (Peer Reviewed Journal)Riedelsberger, J., Miller, J.K., Valdebenito-Maturana, B., Pineros, M., Gonzales, W., Dreyer, I. 2021. Plant HKT Channels: an updated view on structure, function and gene regulation. International Journal of Molecular Sciences. 22(4):1892. https://doi.org/10.3390/ijms22041892.YSL3-mediated copper distribution is required for fertility, seed size and protein accumulation in Brachypodium
                                                        

- (Peer Reviewed Journal)Sheng, H., Jiang, Y., Ishka, M.R., Chia, J., Dokuchayeva, T., Kavulych, Y., Zavodna, T., Mendoza, P.N., Huang, R., Smieshka, L.M., Miller, J., Woll, A.R., Terek, O.I., Romanyuk, N.D., Pineros, M., Zho, Y., Vatamaniuk, O.K. 2021. YSL3-mediated copper distribution is required for fertility, seed size and protein accumulation in Brachypodium. Plant Physiology. 186(1):655-676. https://doi.org/10.1093/plphys/kiab054.Indole-3-glycerolphosphate synthase, a branchpoint for the biosynthesis of tryptophan, indole, and benzoxazinoids in maize
                                                        

- (Peer Reviewed Journal)Richter, A., Powell, A.F., Mirzaei, M., Wang, L.J., Movahed, N., Miller, J.K., Pineros, M., Jander, G. 2021. Indole-3-glycerolphosphate synthase, a branchpoint for the biosynthesis of tryptophan, indole, and benzoxazinoids in maize. The Plant Journal. 106:245-257. https://doi.org/10.1111/tpj.15163.Low additive genetic variation in a trait under selection in domesticated rice
                                                        

- (Peer Reviewed Journal)Karavolias, N.G., Greenberg, A.J., Barrero, L.S., Maron, L.G., Shi, Y., Monteverde, E., Pineros, M., Mccouch, S. 2020. Low additive genetic variation in a trait under selection in domesticated rice. Genes, Genomes, Genetics. 10(7):2435-2443. https://doi.org/10.1534/g3.120.401194.Apple ALMT9 requires a conserved C-terminal domain for malate transport underlying fruit acidity
                                                        

- (Peer Reviewed Journal)Li, C., Dougherty, L., Coluccio, A., Meng, D., El-Sharkwy, I., Borejsza-Wysocka, E., Liang, D., Pineros, M., Xu, K., Cheng, L. 2020. Apple ALMT9 requires a conserved C-terminal domain for malate transport underlying fruit acidity. Plant Physiology. 182(2):992-1006. https://doi.org/10.1104/pp.19.01300.A sugar transporter takes up both hexose and sucrose for sorbitol-modulated in vitro pollen tube growth in apple
                                                        

- (Peer Reviewed Journal)Li, C., Meng, D., Pineros, M., Mao, Y., Dandekar, A.M., Cheng, L. 2020. A sugar transporter takes up both hexose and sucrose for sorbitol-modulated in vitro pollen tube growth in apple. The Plant Cell. 32:449-469. https://doi.org/10.1105/tpc.19.00638.Signal coordination prior to, during, and after stomatal closure in response to drought stress

- (Peer Reviewed Journal)Huber, A., Melcher, P., Pineros, M., Setter, T., Bauerle, T. 2019. Signal coordination prior to, during, and after stomatal closure in response to drought stress. New Phytologist. 224:675-688.Extracellular cation binding pocket is essential for ion conduction of OsHKT2;2

- (Peer Reviewed Journal)Riedelsberger, J., Vergara-Jaque, A., Pineros, M., Dreyer, I., Gonzalez, W. 2019. Extracellular cation binding pocket is essential for ion conduction of OsHKT2;2. Biomed Central (BMC) Plant Biology. 19(1);316.Cryo-EM structure of OSCA1.2 from Oryza sativa: Mechanical basis of potential membrane hyperosmolality-gating

- (Peer Reviewed Journal)Cryo-EM structure of OSCA1.2 from Oryza sativa: Mechanical basis of potential membrane hyperosmolality-gating

- (Peer Reviewed Journal)Maity, K., Heumann, J., Mcgrath, A., Kopcho, N., Hsu, P., Lee, C., Mapes, J., Garza, D., Krishnan, S., Morgan, G., Hendargo, K., Klose, T., Rees, S., Medrano-Soto, A., Saier, M., Pineros, M., Komives, E., Schroeder, J., Chang, G., Stowell, M. 2019. Cryo-EM structure of OSCA1.2 from Oryza sativa: Mechanical basis of potential membrane hyperosmolality-gating. Proceedings of the National Academy of Sciences. 116(28):14309-14318.Emerging pleiotropic mechanisms underlying aluminum resistance and phosphorus acquisition on acidic soils

- (Peer Reviewed Journal)Magalhaes, J., Pineros, M., Maciel, L., Kochian, L. 2018. Emerging pleiotropic mechanisms underlying aluminum resistance and phosphorus acquisition on acidic soils. Frontiers in Plant Science. 9:1420.Two citrate transporters coordinately regulate citrate secretion from rice bean root tip under aluminum stress
                                                        

- (Peer Reviewed Journal)Liu, M., Lou, H., Chen, W., Pineros, M., Xu, J., Fan, W., Kochian, L., Yang, J., Zheng, S. 2018. Two citrate transporters coordinately regulate citrate secretion from rice bean root tip under aluminum stress. Plant, Cell & Environment. 41:809-822. https://doi.org/10.1111/pce.13150.Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor
                                                        

- (Peer Reviewed Journal)Doshim, R., McGrath, A., Pineros, M., Szewczyk, P., Garza, D., Kochian, L., Chang, G. 2017. Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor. Scientific Reports. 7(17996). https://doi.org/10.1038/s41598-017-18146-8.Aluminum resistance transcription factor 1 (ART1) contributes to natural variation in rice aluminum resistance
                                                        

- (Peer Reviewed Journal)Arbelaez, J., Maron, L., Jobe, T., Pineros, M., Rebelo, A., Famoso, A., Ma, Q., Fei, Z., Kochian, L., McCouch, S. 2017. Aluminum resistance transcription factor 1 (ART1) contributes to natural variation in rice aluminum resistance. Plant and Soil. 1(4):1-19. https://doi.org/10.1002/pld3.14.Loss-of-function mutation of the calcium sensor CBL1 increases aluminum sensitivity in Arabidopsis
                                                        

- (Peer Reviewed Journal)Ligaba, A., Fei, Z., Liu, J., Xu, Y., Jia, X., Shaff, J., Lee, S., Luan, S., Kudla, J., Kochian, L.V., Pineros, M. 2017. Loss-of-function mutation of the calcium sensor CBL1 increases aluminum sensitivity in Arabidopsis. New Phytologist. 214(2):830-841. https://doi.org/10.1111/nph.14420.The ALMT family of organic acid transporters in plants and their involvement in detoxification and nutrient security
                                                        

- (Review Article)Sharma, T., Dreyer, I., Kochian, L.V., Pineros, M. 2016. The ALMT family of organic acid transporters in plants and their involvement in detoxification and nutrient security. Frontiers in Plant Science. 7(1488). https://doi.org/10.3389/fpls.2016.01488.An Arabidopsis ABC transporter mediates phosphate deficiency-induced remodeling of root architecture by modulating iron homeostasis in roots
                                                        

- (Peer Reviewed Journal)Dong, J., Pineros, M., Li, X., Yang, H., Liu, Y., Murphy, A., Kochian, L.V., Liu, D. 2017. An Arabidopsis ABC transporter mediates phosphate deficiency-induced remodeling of root architecture by modulating iron homeostasis in roots. Molecular Plant. 10(2):244-259. https://doi.org/10.1016/j.molp.2016.11.001.Redefining ‘stress resistance gene’, and why it matters
                                                        

- (Review Article)Maron, L., Pineros, M., Kochian, L.V., Mccouch, S. 2016. Redefining ‘stress resistance gene’, and why it matters. Journal of Experimental Botany. 67(19):5588-5591. https://doi.org/10.1093/jxb/erw323.The raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for innate immunity and abiotic stress response

- (Peer Reviewed Journal)Brauer, E.K., Ahsan, N., Dale, R., Kato, N., Coluccio, A.E., Pineros, M., Kochian, L.V., Thelen, J.J., Popescu, S. 2016. The raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for innate immunity and abiotic stress response. Plant Physiology. doi: 10.1104/pp.16.00035.Advances and considerations in technologies for growing, imaging, and analyzing 3-D root system architecture

- (Peer Reviewed Journal)Pineros, M., Larson, B., Shaff, J., Schneider, D.J., Falcao, A., Lixing, Y., Clark, R.T., Craft, E.J., Davis, T.W., Pradier, P., Liu, J., Assaranurak, I., Susan, M., Sturrock, C., Bennett, M., Kochian, L.V. 2016. Advances and considerations in technologies for growing, imaging, and analyzing 3-D root system architecture. Journal of Integrative Plant Biology. 58(3):230-241.Plant adaptation to acid soils: the molecular basis for crop aluminum resistance

- (Review Article)Kochian, L.V., Pineros, M., Liu, J., Magalhaes, J. 2015. Plant adaptation to acid soils: the molecular basis for crop aluminum resistance. Annual Reviews of Plant Biology. 66:571-598. DOI: 10.1146/annualrev-arplant-043014-114822.OPT3 is a phloem-specific iron transporter that is essential for systemic iron signaling and redistribution of iron and cadmium in arabidopsis

- (Peer Reviewed Journal)Zhiyang, Z., Gayomba, S.R., Jung, H., Vimalakumar, N.K., Pineros, M., Rutzke, M., Danku, J., Lahner, B., Punshon, T., Guerinot, M., Salt, D.D., Kochian, L.V., Vatamaniuk, O.K. 2014. OPT3 is a phloem-specific iron transporter that is essential for systemic iron signaling and redistribution of iron and cadmium in arabidopsis. The Plant Cell. DOI: http:dx.doi.org/10.1105/tpc.114.123737.Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice

- (Peer Reviewed Journal)Wang, X., Wang, Z., Pineros, M., Wang, Z., Wang, W., Li, C., Wu, Z., Kochian, L.V., Wu, P. 2014. Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice. Plant Cell and Environment. 37(5):1159-1170. DOI: 10.1111/pce.201437.issue-5/issuetoc.The role of aluminum sensing and signaling in plant aluminum resistance

- (Review Article)Liu, J., Pineros, M., Kochian, L.V. 2014. The role of aluminum sensing and signaling in plant aluminum resistance. Journal of Integrative Plant Biology. 56(3):221-230. DOI: 10.1111/jipb.12162Physiological and molecular analysis of selected Kenyan maize lines for aluminum tolerance

- (Peer Reviewed Journal)Matonyei, T., Cheprot, R., Liu, J., Pineros, M., Shaff, J., Gudo, S., Were, B., Magalhaes, J., Kochian, L.V. 2014. Physiological and molecular analysis of selected Kenyan maize lines for aluminum tolerance. Plant Journal. 377:357-367.Functional, structural and phylogenetic analysis of domains underlying the Al-sensitivity of the aluminium-activated malate/anion transporter, TaALMT1
                                                        

- (Peer Reviewed Journal)Ligaba, A., Dreyer, I., Margaryan, A., Schneider, D.J., Kochian, L.V., Pineros, M. 2013. Functional, structural and phylogenetic analysis of domains underlying the Al-sensitivity of the aluminium-activated malate/anion transporter, TaALMT1. Plant Journal. 76(6):766-780.  DOI: 10.1111/tpj.12332Molecular and physiological mechanisms of plant tolerance to toxic metals
                                                        

- (Book / Chapter)Milner, M., Pineros, M., Kochian, L.V. 2014. Molecular and physiological mechanisms of plant tolerance to toxic metals. In: Jenks, M.A. and Hasegawa, P.M., editors. Plant Abiotic Stress, Second Edition. Hoboken, NJ: John Wiley & Sons, Inc. p. 179-196.Aluminum tolerance in sorghum and maize
                                                        

- (Book / Chapter)Magalhaes, J.V., Maron, L.G., Pineros, M., Guimaraes, C.T., Kochian, L.V. 2013. Aluminum tolerance in sorghum and maize. In: Varshney, R., Ruberosa, R., editors. Translational genomics for crop breeding: improvement for abiotic stress, quality and yield improvement. Volume 2. Chichester, UK. Wiley and Sons Ltd. p. 83-98. DOI: 10.1002/9781118728482.ch6Aluminum tolerance is associated with higher MATE1 gene copy-number in maize
                                                        

- (Peer Reviewed Journal)Maron, L., Guimareas, C., Matias, K., Albert, P.S., Birchler, J.A., Bradbury, P., Buckler IV, E.S., Coluccio, A.E., Danilova, T.V., Kudrna, D., Magalhaes, J.V., Pineros, M., Schatz, M.C., Wing, R., Kochian, L.V. 2013. Aluminum tolerance is associated with higher MATE1 gene copy-number in maize. Proceedings of the National Academy of Sciences. 110(13):5241-5246.Low pH, aluminum and phosphorus coordinately regulate malate exudation through GmALMT1 to improve soybean adaptation to acid soils

- (Peer Reviewed Journal)Liang, C., Pineros, M., Tian, J., Yao, Z., Sun, L., Liu, J., Shaff, J., Liao, H., Kochian, L.V. 2013. Low pH, aluminum and phosphorus coordinately regulate malate exudation through GmALMT1 to improve soybean adaptation to acid soils. Plant Physiology. 161:1347-1361.Incomplete transfer of accessory loci influencing SbMATE expression underlies genetic background effects for aluminum tolerance in sorghum

- (Peer Reviewed Journal)Melo, J., Lana, U., Pineros, M., Alves, V., Guimaraes, C., Liu, J., Zheng, Y., Zhong, S., Fei, Z., Maron, L., Schaeffert, R., Kochian, L.V., Magalhaes, J. 2013. Incomplete transfer of accessory loci influencing SbMATE expression underlies genetic background effects for aluminum tolerance in sorghum. Plant Journal. 73(2):276-288.Maize ZmALMT2 is a root anion transporter that mediates constitutive root malate efflux

- (Peer Reviewed Journal)Ligaba, A., Maron, L., Shaff, J.E., Kochian, L.V., Pineros, M. 2012. Maize ZmALMT2 is a root anion transporter that mediates constitutive root malate efflux. Plant, Cell & Environment. 35(7):1365-3040.Title A de novo synthesis citrate transporter VuMATE confers aluminum resistance in rice bean (vigna umbellata)

- (Peer Reviewed Journal)Yang, X., Yang, J., Pineros, M., Kochian, L.V., Zheng, S. 2011. Title A de novo synthesis citrate transporter VuMATE confers aluminum resistance in rice bean (vigna umbellata). Plant Cell and Environment. 34:2138-2148.Two functionally distinct members of the MATE (multidrug and toxic compound extrusion) family of transporters potentially underlie two major Al tolerance QTL in maize

- (Peer Reviewed Journal)Maron, L., Guimaraes, C., Pineros, M., Magalhaes, J., Pleiman, J., Mao, C., Kochian, L.V. 2010. Two functionally distinct members of the MATE (multidrug and toxic compound extrusion) family of transporters potentially underlie two major Al tolerance QTL in maize. Plant Journal. 61:728-740.Phosphorylation at S384 regulates the activity of the TaALMT1 malate transporter that underlies aluminum resistance in wheat

- (Peer Reviewed Journal)Ligaba, A., Kochian, L.V., Pineros, M. 2009. Phosphorylation at S384 regulates the activity of the TaALMT1 malate transporter that underlies aluminum resistance in wheat. Plant Journal. 60:411-423.Evaluation of aluminum tolerance in grapevine rootstocks

- (Peer Reviewed Journal)Cancado, G., Ribeiro, P., Pineros, M., Myiata, L., Alvarenga, A., Villa, F., Pasqual, M., Purgatto, E. 2009. Evaluation of aluminum tolerance in grapevine rootstocks. Vitis. 48(4):167-173.Recent Advances on the Molecular Basis of Crop Aluminum Resistance

- (Proceedings)Kochian, L.V., Magalhaes, J., Liu, J., Hoekenga, O., Pineros, M. 2009. Recent Advances on the Molecular Basis of Crop Aluminum Resistance. Meeting Proceedings.Maize aluminum tolerance

- (Book / Chapter)Kochian, L.V., Hoekenga, O., Magalhaes, J., Pineros, M. 2009.  Maize aluminum tolerance. In: Bennetzen, J.L., Hale, E.S.C., editors. Handbook of Maize: It's Biology. New York, NY: Springer. p. 367-380.Novel properties of the wheat aluminum tolerance organic acid transporter (TaALMT1) revealed by electrophysiological characterization in Xenopus oocytes: Functional and structural implications

- (Peer Reviewed Journal)Pineros, M., Kochian, L.V. 2008. Novel properties of the wheat aluminum tolerance organic acid transporter (TaALMT1) revealed by electrophysiological characterization in Xenopus oocytes: Functional and structural implications. Plant Physiology. 147:2131-2146.Characterization of AtALMT1 expression in aluminum inducible malate release and its role for rhizotoxic stress in Arabidopsis

- (Peer Reviewed Journal)Kobayashi, Y., Hoekenga, O., Ito, H., Nakashima, M., Saito, S., Shaff, J., Maron, L., Pineros, M., Kochian, L.V., Koyama, H. 2008. Characterization of AtALMT1 expression in aluminum inducible malate release and its role for rhizotoxic stress in Arabidopsis. Plant Physiology. 145:843-852.Not all ALMT1-type transporters mediate aluminum-activated organic acid responses: The case of AmALMT1-an anion selective transporter

- (Peer Reviewed Journal)Pineros, M., Cancado, G., Maron, L., Lyi, S., Menossi, M., Kochian, L.V. 2008. Not all ALMT1-type transporters mediate aluminum-activated organic acid responses: The case of AmALMT1-an anion selective transporter. Plant Journal. 53:352-367.Elucidating the Molecular Determinants of Aluminum Tolerance in Sorghum, Maize and Rice

- (Abstract Only)Kochian, L.V., Magalhaes, J., Liu, J., Pineros, M., Maron, L. 2008. Elucidating the Molecular Determinants of Aluminum Tolerance in Sorghum, Maize and Rice. Plant and Animal Genome Conference.The effect of plant cadmium and zinc status on root and shoot heavy metal accumulation in the heavy metal hyperaccumulator, Thlaspi caerulescens

- (Peer Reviewed Journal)Papoyan, A., Pineros, M., Kochian, L.V. 2007. The effect of plant cadmium and zinc status on root and shoot heavy metal accumulation in the heavy metal hyperaccumulator, Thlaspi caerulescens. New Phytologist. 175:51-58.Molecular Cloning and Characterization of AltSB, A Major Aluminum Tolerance Gene in Sorghum

- (Abstract Only)Liu, J., Magalhaes, J., Guimaraes, C., Lana, U., Alves, V., Hoekenga, O., Shaff, J., Pineros, M., Wang, Y., Klein, P., Schaffert, R., Kochian, L.V. 2007. Molecular Cloning and Characterization of AltSB, A Major Aluminum Tolerance Gene in Sorghum [abstract]. Plant and Animal Genome VX Conference Abstracts. p. 59.


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