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                    U.S. DEPARTMENT OF AGRICULTURE
 National Clonal Germplasm Repository for Citrus: Riverside, CA
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ARS Home Pacific West Area Riverside, California National Clonal Germplasm Repository for Citrus People & Locations







Manjunath L Keremane

                                                                (PhD)
                        National Clonal Germplasm Repository for Citrus

                        




                        
                        Plant Pathologist
                        Phone: (951) 827-6981
                        
                        
                        Fax: (Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            **Publications**
                                                (Clicking on the reprint icon
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                                                    will take you to the publication reprint.)NexGen Sequencing, and development of a pipeline as a tool for the detection and discovery of citrus pathogens to facilitate safer germplasm exchange
                                                        

- (Peer Reviewed Journal)Keremane, M.L., Singh, K., Ramadugu, C., Skaggs, T.H., Krueger, R. 2024. NexGen Sequencing, and development of a pipeline as a tool for the detection and discovery of citrus pathogens to facilitate safer germplasm exchange. Plants. https://doi.org/10.3390/plants13030411.Analyzing Huanglongbing-resistant pummelo from India.

- (Abstract Only)New pathogen testing tools for safe movement of citrus germplasm in the Huanglongbing (HLB) era

- (Abstract Only)Analysis of r-genes in Australian limes in comparison to commercial citrus cultivars

- (Abstract Only)Navigating the genetic bottleneck of HLB: exploring the genomes of Australian limes for development of disease resistance

- (Abstract Only)An improved reference gene for detection of “Candidatus Liberibacter asiaticus” associated with citrus huanglongbing by qPCR and digital droplet PCR assays
                                                        

- (Peer Reviewed Journal)Keremane, M.L., McCollum, T.G., Roose, M.L., Lee, R.F., Ramadugu, C. 2021. An improved reference gene for detection of “Candidatus Liberibacter asiaticus” associated with citrus huanglongbing by qPCR and digital droplet PCR assays. Plants. 10(10). Article 2111. https://doi.org/10.3390/plants10102111.Incidence of Asiatic citrus canker on Trifoliate orange and its hybrid accessions in a Florida field planting
                                                        

- (Peer Reviewed Journal)Stover, E.W., Ramadugu, C., Roose, M., Krystel, J.A., Lee, R.F., Keremane, M.L. 2021. Incidence of Asiatic citrus canker on Trifoliate orange and its hybrid accessions in a Florida field planting. Journal of the American Society for Horticultural Science. 56(5):525–531. https://doi.org/10.21273/HORTSCI15684-20.Use of young plants for biological indexing of graft transmissible pathogens of citrus
                                                        

- (Peer Reviewed Journal)Lee, R., Keremane, M.L., Ramadugu, C. 2021. Use of young plants for biological indexing of graft transmissible pathogens of citrus. Crop Protection Journal. 143. Article 105524. https://doi.org/10.1016/j.cropro.2020.105524.In-grove spatio-temporal spread of citrus huanglongbing and its psyllid vector in relation to rain
                                                        

- (Peer Reviewed Journal)Shimwela, M.M., Halbert, S.E., Keremane, M.L., Mears, P., Singer, B.H., Lee, W.S., Jones, J.B., Ploetz, R.C., Van Bruggen, A.H. 2019. In-grove spatio-temporal spread of citrus huanglongbing and its psyllid vector in relation to rain. Journal of Phytopathology. 109(3):418-427. https://doi.org/10.1094/PHYTO-03-18-0089-R.Novel development of a lateral flow immunoassay for rapid field detection of citrus tristeza virus

- (Peer Reviewed Journal)Maheshwari, Y., Selvaraj, V., Hajeri, S., Ramadugu, C., Keremane, M.L., Yokomi, R.K. 2017. Novel development of a lateral flow immunoassay for rapid field detection of citrus tristeza virus. Molecular Biotechnology. doi: 10.1007/s12600-017-0591-0.Changes in anthocyanin production during domestication of citrus

- (Peer Reviewed Journal)Changes in anthocyanin production during domestication of Citrus
                                                        

- (Peer Reviewed Journal)Butelli, E., Garcia-Lor, A., Licciardello, C., Las Casas, G., Hill, L., Recupero, G.R., Keremane, M.L., Ramadugu, C., Krueger, R., Xu, Q., Deng, X., Fanciullino, A., Frocelicher, Y., Navarro, L., Martin, C. 2017. Changes in anthocyanin production during domestication of Citrus. Plant Physiology. 173:2225-2242. https://doi.org/10.1104/pp.16.01701.Huanglongbing: An Overview of a Complex Pathosystem Ravaging the World's Citrus

- (Peer Reviewed Journal)Da Graca, J.V., Douhan, G.W., Halbert, S., Keremane, M.L., Lee, R., Vidalakais, G., Zhao, H. 2016. Huanglongbing: An overview of a complex pathosystem ravaging the world's Citrus. Journal of Integrative Plant Biology. doi: 10.1111/jipb.12437.Apparent tolerance to huanglongbing in citrus and citrus-related germplasm

- (Peer Reviewed Journal)Miles, G., Stover, E., Keremane, M., Ramadugu, C., Lee, R.F. 2017. Apparent tolerance to huanglongbing in citrus and citrus-related germplasm. HortScience. 52:31-39.Early detection of CLas infections in citrus

- (Abstract Only)Long term field evaluation reveals HLB resistance in Citrus relatives

- (Peer Reviewed Journal)Keremane, M.L., Ramadugu, C., Halbert, S., Duan, Y., Roose, M., Stover, E.W., Lee, R. 2016. Long term field evaluation reveals HLB resistance in Citrus relatives. Plant Disease. http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-03-16-0271-RE.Variation within and among laboratories in detection of Candidatus Liberibacter asiaticus using qPCR

- (Abstract Only)Lateral flow immunoassay for the rapid detection of citrus tristeza virus

- (Abstract Only)Maheshwari, Y., Selvaraj, V., Ramadugu, C., Keremane, M.L., Hajeri, S., Yokomi, R.K. 2016. Lateral flow immunoassay for the rapid detection of citrus tristeza virus. Conference of International Organization of Citrus Virologists. p.57.Growth measurements and disease severity metrics of citrus identifies resistance and tolerance to Huanglongbing (HLB) in a Florida field planting

- (Abstract Only)Miles, G.P., Stover, E.W., Ramadugu, C., Keremane, M.L., Lee, R.F. 2016. Growth measurements and disease severity metrics of citrus identifies resistance and tolerance to Huanglongbing (HLB) in a Florida field planting [abstract]. 129th Annual Meeting of the Florida State Horticultural Society. June 12-14, 2016, Stuart, Florida. Abstract number G11.Developing resistance to HLB

- (Trade Journal)Ramadugu, C., Keremane, M.L., Mccollum, T.G., Hall, D.G., Roose, M. 2016. Developing resistance to HLB. Citrograph. 7(2):46-51.Citrus huanglongbing (HLB) discoveries in California respective in 2015 and 2012 are different genotypes of candidatus liberibacter asiaticus (CLas) by double-locus genomic variations analysis

- (Peer Reviewed Journal)Zhonge, Y., Rascoe, J., Kumagai, L.B., Keremane, M.L., Nakhla, M.K. 2016. Citrus huanglongbing (HLB) discoveries in California respective in 2015 and 2012 are different genotypes of candidatus liberibacter asiaticus (CLas) by double-locus genomic variations analysis. Plant Disease. 100(3):647.Draft genome sequence of “Candidatus Liberibacter asiaticus” from a citrus tree in San Gabriel, California

- (Peer Reviewed Journal)Wu, F., Kumagai, L., Liang, G., Deng, X., Zheng, Z., Keremane, M.L., Chen, J. 2015. Draft genome sequence of “Candidatus Liberibacter asiaticus” from a citrus tree in San Gabriel, California. Genome Announcements. 3(6):e01508-15. doi: 10.1128/genomeA.01508-15.Genetic analysis of citron (Citrus medica L.) using simple sequence repeats and single nucleotide polymorphisms

- (Peer Reviewed Journal)Ramadugu, C., Keremane, M.L., Hu, X., Karp, D., Federici, C.T., Kahn, T., Roose, M.L., Lee, R.F. 2015. Genetic analysis of citron (Citrus medica L.) using simple sequence repeats and single nucleotide polymorphisms. Scientia Horticulturae. 195:124-137.Citrus Huanglongbing tolerance in Australian Citrus Relatives, Microcitrus and Eremocirus

- (Abstract Only)Whole-genome sequence of “Candidatus Liberibacter solanacearum” strain R1 from California

- (Peer Reviewed Journal)Zheng, Z., Clark, N., Keremane, M.L., Lee, R.F., Wallis, C.M., Deng, X., Chen, J. 2014. Whole-genome sequence of “Candidatus Liberibacter solanacearum” strain R1 from California. Genome Announcements. 2(6):e01353-14.A rapid field detection system for citrus huanglongbing associated ‘Candidatus Liberibacter asiaticus’ from the psyllid vector, diaphorina citri kuwayama and its implications in disease management

- (Peer Reviewed Journal)Keremane, M.L., Ramadugu, C., Jenkins, D., Kubota, R., Shibata, S., Hall, D.G., Roose, M., Lee, R.F., Rodriguez, E. 2014. A rapid detection system for citrus huanglongbing associated ‘Candidatus Liberibacter asiaticus’ from Diaphorina citri. Crop Protection. 68:41-48.Screening Citrus germplasm for tolerance to HLB and Diaphorina citri--progress

- (Trade Journal)Detection of citrus huanglongbing-associated ‘Candidatus Liberibacter asiaticus’ in citrus and Diaphorina citri in Pakistan, season variability, and implications for disease management

- (Peer Reviewed Journal)Razi, M.F., Keremane, M.L., Ramadugu, C., Roose, M., Kahn, I., Lee, R.F. 2014. Detection of citrus huanglongbing-associated ‘Candidatus Liberibacter asiaticus’ in citrus and Diaphorina citri in Pakistan, season variability, and implications for disease management. Phytopathology. 104:257-268.Mild strain cross protection of tristeza: A review of research to protect against decline on sour orange in Florida and a look at the future

- (Peer Reviewed Journal)Lee, R.F., Keremane, M.L. 2013. Mild strain cross protection of tristeza: A review of research to protect against decline on sour orange in Florida and a look at the future. Frontiers in Virology. 4:259. DOI: 10.3389/frmicb.2013.00259.A six nuclear gene phylogeny of Citrus (Rutaceae) taking into account hybridization and lineage sorting

- (Peer Reviewed Journal)Ramadugu, C., Pfiel, B., Keremane, M.L., Lee, R.F., Maureira-Butler, I., Roose, M. 2013. A six nuclear gene phylogeny of Citrus (Rutaceae) taking into account hybridization and lineage sorting. PLoS ONE. 8(7) e68410. DOI:10.1371/journal.pone.0068410Incidence of huanglongbing-associated ‘Candidatus Liberibacter Asiaticus’ in Diaphorina citri (Hemiptera: Psyllidae) collected from plants for sale in Florida

- (Peer Reviewed Journal)Halbert, S., Keremane, M.L., Ramadugu, C., Lee, R.F. 2012. Incidence of huanglongbing-associated ‘Candidatus Liberibacter Asiaticus’ in Diaphorina citri (Hemiptera: Psyllidae) collected from plants for sale in Florida. Florida Entomologist. 95(3):617-624.Improved real-time PCR detection of Candidatus Liberibacter asiaticus from citrus and psyllid hosts by targeting the intragenic tandem repeats of its prophage genes

- (Peer Reviewed Journal)Morgan, J.K., Zhou, L., Li, W., Shatters, R.G., Keremane, M.L., Duan, Y. 2012. Improved real-time PCR detection of Candidatus Liberibacter asiaticus from citrus and psyllid hosts by targeting the intragenic tandem repeats of its prophage genes. Molecular and Cellular Probes. 26:90-98.In vivo generated Citrus exocortis viroid progeny variants display a range of phenotypes with altered levels of replication, systemic accumulation and pathogenicity

- (Peer Reviewed Journal)Hajeri, S., Ramadugu, C., Keremane, M.L., Ng, J., Lee, R.F., Vidalakis, G. 2011. In vivo generated Citrus exocortis viroid progeny variants display a range of phenotypes with altered levels of replication, systemic accumulation and pathogenicity. Virology. 417(2):400-409. DOI:10.1016/j.virol.2011.06.013Behavioral responses of adult potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae), to potato germplasm and transmission of Candidatus Liberibacter psyllaurous

- (Peer Reviewed Journal)Butler, C., Gonzalez, B., Keremane, M.L., Lee, R.F., Novy, R.G., Miller, J., Trumble, J. 2011. Behavioral responses of adult potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae), to potato germplasm and transmission of Candidatus Liberibacter psyllaurous. Crop Protection Journal. 30(9):p1233-1238.“Candidatus liberibacter sp.”, without koch's postulates completed, can the bacterium be considered as the causal agent of citrus Huanglongbing (yellow shoot disease)?

- (Peer Reviewed Journal)Chen, J., Civerolo, E.L., Lee, R.F., Jones, J., Deng, X., Hartung, J.S., Keremane, M.L., Brlansky, R. 2011. “Candidatus liberibacter sp.”, without koch's postulates completed, can the bacterium be considered as the causal agent of citrus Huanglongbing (yellow shoot disease)?. Acta Phytopathologica Sinica. 41(2):113-117.Nucleotide sequence and genome organization of Dweet mottle virus and its relationship to members of the family Betaflexiviridae

- (Peer Reviewed Journal)Hajeri, S., Ramadugu, C., Keremane, M.L., Vidalakis, G., Lee, R.F. 2010. Nucleotide sequence and genome organization of Dweet mottle virus and its relationship to members of the family Betaflexiviridae. Archives of Virology. 155(9):1523–1527.Trailers transporting oranges to processing plants move Asian citrus psyllids

- (Peer Reviewed Journal)Halbert, S.E., Keremane, M.L., Ramadugu, C., Brodie, M.W., Webb, S.E., Lee, R.F. 2010. Trailers transporting oranges to processing plants move Asian citrus psyllids. Florida Entomologist. 93(1)p33-38.Evaluation of Quantitative Real-Time PCR Assays for Detection of Citrus Greening

- (Proceedings)Turechek, W., Gottwald, T.R., Hartung, J.S., Hilf, M.E., Keremane, M.L., Lin, H., Shatters, R.G., Irey, M., Sieburth, P., Brlansky, R., Dagraca, J., Graham, J., Kunta, M., Roberts, P., Rogers, M., Sun, X., Wang, N. 2009. Evaluation of Quantitative Real-Time PCR Assays for Detection of Citrus Greening. Workshop Proceedings. 158-160.Gene expression analysis to understand cold tolerance in citrus

- (Abstract Only)Phylogenetic analysis of Rutaceous plants based on single nucleotide polymorphism in chloroplast and nuclear gene sequences

- (Abstract Only)Production of Polyclonal Antibodies to the Recombinant Coat Protein of Citrus tristeza virus and Their Effectiveness for Virus Detection

- (Peer Reviewed Journal)Iracheta-Cardenas, M.M., Sandoval-Alejo, B.D., Roman-Calderon, M.E., Keremane, M.L., Lee, R.F., Rocha-Pena, M.A. 2008. Production of Polyclonal Antibodies to the Recombinant Coat Protein of Citrus tristeza virus and Their Effectiveness for Virus Detection. Journal of Phytopathology, 156: 243-250Detection of Candidatus Liberibacter asiaticus in Diaphorina citri and its importance in the management of Citrus Huanglongbing in Florida

- (Peer Reviewed Journal)Keremane, M.L., Halbert, S.E., Ramadugu, C., Webb, S., Lee, R.F. 2008. Detection of Candidatus Liberibacter asiaticus in Diaphorina citri and its importance in the management of Citrus Huanglongbing in Florida. Phytopathology, 98: 387-396Phylogenetic analysis of Citrus based on sequences of six nuclear genes

- (Abstract Only)Quantitative Detection of Citrus tristeza virus (CTV) in Citrus and Aphids by Real-time Reverse Transcription-PCR (TaqMan®)

- (Peer Reviewed Journal)Saponari, M., Keremane, M.L., Yokomi, R.K. 2008. Quantitative Detection of Citrus tristeza virus (CTV) in Citrus and Aphids by Real-time Reverse Transcription-PCR (TaqMan®). Journal of Virological Methods. 147:43-53.Use of Psyllids in Early Identification of Huanglongbing Disease of Citrus

- (Abstract Only)Keremane, M.L., Halbert, S., Ramadugu, C., Webb, S., Lee, R.F. 2007. Use of Psyllids in Early Identification of Huanglongbing Disease of Citrus. Phytopathology, 97, No7: S70.Incidence of Huanglongbing in Psyllids Under Natural Conditions in Florida

- (Abstract Only)Ramadugu, C., Keremane, M.L., Ramos, C., Halbert, S., Lee, R.F. 2007. Incidence of Huanglongbing in Psyllids Under Natural Conditions in Florida. Phytopathology, 97, No7: S96.Psyllids as A Tool in Evaluating the Efficiency of Different Management Practices for Control of Citrus Huanglongbing Disease

- (Abstract Only)Keremane, M.L., Harakava, R., Ramadugu, C., Halbert, S., Yamamoto, P., Lee, R.F. 2007. Psyllids as A Tool in Evaluating the Efficiency of Different Management Practices for Control of Citrus Huanglongbing Disease. Phytopathology, 97, No7: S70.NPDN Citrus Greening Diagnostic and Detection Efforts

- (Abstract Only)Harmon, C.L., Keremane, M.L., Schubert, T.S. 2007. NPDN Citrus Greening Diagnostic and Detection Efforts. National Plant Diagnosic Network, 2007 National Meeting.The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus (CCLV) and its Genome Organization

- (Abstract Only)Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus, (CCLV) and its Genome Organization. Phytopathology 97: S 174Evaluación de Anticuerpos Desarrollados Contra la Proteína Recombinante de la Cápside del Virus Tristeza de los Cítricos

- (Peer Reviewed Journal)Sandoval-Alejo, B.D., Iracheta-Cardenas, M.M., Keremane, M.L., Lee, R.F., Rocha-Pena, M.A. 2006. Evaluación de Anticuerpos Desarrollados Contra la Proteína Recombinante de la Cápside del Virus Tristeza de los Cítricos. Revista Mexicana de Fitopatologia, Vol. 24: 137-144.Leprosis in Guatemala

- (Abstract Only)Palmieri, M., Donis, I., Salazar, A., Blanco, S., Porres, M., Brlansky, R.H., Guerra-Moreno, A.S., Keremane, M.L., Lee, R.F. 2007. Leprosis in Guatemala. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 510.Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection

- (Abstract Only)Keremane, M.L., Ramadugu, C., Warren, C., Niblett, C.L., Lee, R.F. 2007. Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 487.Bacterial Expression and Purification of a Protein From Citrus Leprosis Virus (CiLV)

- (Abstract Only)Rangel, E., Keremane, M.L., Guerra-Moreno, A.S., Brlansky, R.H., Lee, R.F. 2007. Bacterial Expression and Purification of a Protein From Citrus Leprosis Virus (CiLV). International Organization of Citrus Virologists Proceedings, Pg. 510.HUANGLONGBING, CITRUS GREENING:  PREPARING FOR THE FUTURE

- (Trade Journal)Lee, R.F., Keremane, M.L., Williams, M., Vidalakis, G. 2006. Huanglongbing, citrus greening:  preparing for the future. Subtropics newsletter.Citrus Leprosis Symptoms Can be Associated with the Presence of Two Different Viruses:  Cytoplasmic and Nuclear, the Former Having a Multipartite RNA Genome

- (Proceedings)Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. Citrus Leprosis Symptoms Can be Associated with the Presence of Two Different Viruses:  Cytoplasmic and Nuclear, the Former Having a Multipartite RNA Genome. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 230-239.Molecular Characterization of Citrus tristeza virus Isolates from Panama

- (Proceedings)Ramos, C., Castillo, J.C., Fernandez, O., Rangel, B., Keremane, M.L., Lee, R.F. 2007. Molecular Characterization of Citrus tristeza virus Isolates from Panama. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 159-164.Citrus Viruses in Guatemala: Application of Laboratory-Based Assays

- (Proceedings)Palmieri, M., Donis, I., Salazar, A.L., Cruz, N., Paniagua, A., Brlansky, R.H., Guerra-Moreno, A., Keremane, M.L., Ballance, P.M., Lee, R.F. 2007. Citrus Viruses in Guatemala: Application of Laboratory-Based Assays. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 386-391.
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