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                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
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ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality People & Locations







Joseph M Bosilevac

(Mick)
                        Meat Safety and Quality

                        




                        
                        Research Microbiologist
                        Phone: (402) 762-4225
                        
                        
                        Fax: (402) 762-4149(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            ProjectsHolistic Tactics to Advance the Microbiological Safety and Quality of the Red Meat Continuum
                                                    In-House Appropriated
                                                                    (D)Accession Number: 440930Evaluation of Media Formulated to Aid in Isolation of Shiga Toxin-producing E. Coli
                                                    Non-Funded Cooperative Agreement
                                                                    (N)Accession Number: 442725Collective Dynamics of Environmental Multispecies Biofilms That Facilitate Sanitizer Tolerance and Dissemination of Foodborne Pathogens
                                                    Reimbursable Cooperative Agreement
                                                                    (R)Accession Number: 438724**Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Antibiotic and sanitizer resistance among Escherichia coli isolated during red meat production

- (Peer Reviewed Journal)Impact of intense sanitization procedures on bacterial communities recovered from floor drains in pork processing plants

- (Peer Reviewed Journal)Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and TT12B: Comprehensive phylogenomic analysis using closed genomes
                                                        

- (Peer Reviewed Journal)Kalalah, A.A., Koenig, S.S., Feng, P.C., Bosilevac, J.M., Bono, J.L., Eppinger, M. 2024. Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and TT12B: Comprehensive phylogenomic analysis using closed genomes. Microorganisms. 12(4). Article 699. https://doi.org/10.3390/microorganisms12040699.Comparative genomics of extra intestinal pathogenic E. coli from human clinical and food samples

- (Abstract Only)Pathogenomes and virulence profiles of representative big six non-O157 serogroup Shiga toxin-producing Escherichia coli
                                                        

- (Peer Reviewed Journal)Kalalah, A.A., Koenig, S.S.K., Bono, J.L., Bosilevac, J.M., Eppinger, M. 2024. Pathogenomes and virulence profiles of representative big six non-O157 serogroup Shiga toxin-producing Escherichia coli. Frontiers in Microbiology. 15. Article 1364026. https://doi.org/10.3389/fmicb.2024.1364026.Insights into the plasticity and virulence of Escherichia coli serogroup O45

- (Abstract Only)Kalalah, A.A., Higgins, A., Koenig, S.S., Bosilevac, J.M., Eppinger, M. 2024. Insights into the plasticity and virulence of Escherichia coli serogroup O45 [abstract]. American Society of Microbiology Microbe. No 5914.Pathogenomes and virulence profiles of representative emerging big six non-O157 serogroups Shiga toxin-producing Escherichia coli

- (Abstract Only)Kalalah, A.A., Konig, S.S., Bono, J.L., Bosilevac, J.M., Eppinger, M. 2024. Pathogenomes and virulence profiles of representative emerging big six non-O157 serogroups Shiga toxin-producing Escherichia coli(abstract). American Society for Microbiology, No. 5613.Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and B: comprehensive phylogenomic analysis using closed genomes

- (Abstract Only)Kalalah, A.A., Koenig, S.S., Feng, P.C., Bosilevac, J.M., Bono, J.L., Eppinger, M. 2024. Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and B: comprehensive phylogenomic analysis using closed genomes [abstract]. American Society for Microbiology. No. 5741.Impact of intense santization on environmental biofilm communities and the survival of Salmonella enterica at a beef processing plant
                                                        

- (Peer Reviewed Journal)Wang, R., Guragain, M., Chitlapilly Dass, S., Palanisamy, V., Bosilevac, J.M. 2024. Impact of intense santization on environmental biofilm communities and the survival of Salmonella enterica at a beef processing plant. Frontiers in Microbiology. 15. 1338600. https://doi.org/10.3389/fmicb.2024.1338600.Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride
                                                        

- (Peer Reviewed Journal)Chen, Q., Palanisamy, V., Wang, R., Bosilevac, J.M., Chitapilly Dass, S. 2024. Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride. Microbiology Spectrum. 12(2). Article 02346-23. https://doi.org/10.1128/spectrum.02346-23.Multiplex high resolution melt curve real-time PCR assay for detection of extended-spectrum Beta-lactam-resistant Shiga toxin-producing E. coli

- (Peer Reviewed Journal)Shotgun metagenomics reveals a highly diverse and communal microbial network present in the drains of three beef-processing plants

- (Peer Reviewed Journal)The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes

- (Abstract Only)Habib, K., Schmidt, J.W., Nichols, C.A., Kang, Q., Bosilevac, J.M., Harhay, D.M., Gragg, S.E. 2022. The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes. [Abstract]. International Association for Food Protection Proceedings. P1-83.Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels

- (Abstract Only)Schmidt, J.W., Fashenpour, E., Soler-Diaz, R., Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Kang, Q., Gragg, S.E., Sanchez-Plata, M. 2023. Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels. [Abstract]. American Meat Science Association Conference Reciprocal Proceedings. No. 117.Characterizing the collective dynamics of microbial interactions in environmental multi-species biofilm using shotgun metagenomics

- (Abstract Only)Post abattoir risk-based meat safety assurance

- (Abstract Only)Bosilevac, J.M., Arthur, T.M., Wang, R. 2023. Post abattoir risk-based meat safety assurance [abstract]. Risk-based meat inspection and integrated meat safety assurance (RIBMINS) 3rd conference, March 29-30, 2023, Bucharest, Romania. P. 9.Shiga toxin-producing Escherichia coli in pork, are they really only a problem for beef?

- (Abstract Only)Bosilevac, J.M., Guragain, M., Wang, R. 2023. Shiga toxin-producing Escherichia coli in pork, are they really only a problem for beef? [Abstract]. Safe Pork Conference, May 15-17, 2023, New Orleans, LA.  Absract No. 16349.Survival of Salmonella enterica and E. coli O157:H7 in environmental biofilms isolated from pork plants as compared to beef plants

- (Abstract Only)Wang, R., Bosilevac, J.M. 2023. Survival of Salmonella enterica and E. coli O157:H7 in environmental biofilms isolated from pork plants as compared to beef plants. [Abstract. Safepork. 14(1).Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum
                                                        

- (Peer Reviewed Journal)Guragain, M., Schmidt, J.W., Dickey, A.M., Bosilevac, J.M. 2023. Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum. Journal of Food Protection. 86(1). Article 100031. https://doi.org/10.1016/j.jfp.2022.100031.Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens
                                                        

- (Peer Reviewed Journal)Valez, F., Bosilevac, J.M., Mishra, A., Singh, P. 2023. Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens. Journal of Microbiological Methods. 204. Article 106632. https://doi.org/10.1016/j.mimet.2022.106632.Characterization of Salmonella strains and environmental microorganisms isolated from a meat plant with Salmonella recurrence
                                                        

- (Peer Reviewed Journal)Wang, R., Dass, S.C., Chen, Q., Guragain, M., Bosilevac, J.M. 2022. Characterization of Salmonella strains and environmental microorganisms isolated from a meat plant with Salmonella recurrence. Meat and Muscle Biology. 6(1). Article 15442. https://doi.org/10.22175/mmb.15442.A review of Shiga-toxin producing Escherichia coli (STEC) contamination in the raw pork production chain
                                                        

- (Peer Reviewed Journal)Haque, M., Bosilevac, J.M., Chaves, B.D. 2022. A review of Shiga-toxin producing Escherichia coli (STEC) contamination in the raw pork production chain. International Journal of Food Microbiology. 377. Article 109832. http://doi.org/10.1016/j.ijfoodmicro.2022.109832.Impact of “Deep Cleaning” sanitization on natural biofilm communities and the survival of Salmonella enterica

- (Abstract Only)Wang, R., Bosilevac, J.M. 2022. Impact of “Deep Cleaning” sanitization on natural biofilm communities and the survival of Salmonella enterica.  [Abstract]. American Society of Microbiology (ASM) Conference on Biofilms 2022. November 13-17, 2022, Charlotte, NC, Control NO. 2022-A-75-ASM-BIO.Development and validation of high-resolution melting assays for the detection of potentially virulent strains of Escherichia coli O103 and O121
                                                        

- (Peer Reviewed Journal)Velez, F.J., Bosilevac, J.M., Delannoy, S., Fach, P., Nagpal, R., Singh, P. 2022. Development and validation of high-resolution melting assays for the detection of potentially virulent strains of Escherichia coli O103 and O121. Food Control. 139. Article 109095. https://doi.org/10.1016/j.foodcont.2022.109095.Characterization and comparison of Salmonella spp. isolated from the mesenteric lymph nodes of cattle and the feedlot environment

- (Abstract Only)Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems
                                                        

- (Peer Reviewed Journal)Guragain, M., Schmidt, J.W., Kalchayanand, N., Dickey, A.M., Bosilevac, J.M. 2022. Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems. Nature Scientific Reports. 12. Article 5305. https://doi.org/10.1038/s41598-022-09274-x.Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21
                                                        

- (Peer Reviewed Journal)Allué-Guardia, A., Koenig, S.S., Martinez, R.A., Rodriguez, A.L., Bosilevac, J.M., Feng, P., Eppinger, M. 2022. Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21. Microbial Genomics. 8. Article 000796. https://doi.org/10.1099/mgen.0.000796.Complete closed genome sequence of extremely heat resistant escherichia coli AW1.7

- (Research Notes)Identification of phosphoglucomutase as an enteropathogen growth stimulating factor

- (Research Notes)Bosilevac, J.M., Richardson, A.J., Olstein, A.D. 2021. Identification of phosphoglucomutase as an enteropathogen growth stimulating factor. Nutrition Research and Food Science Journal. 4(1):1-12.Assessing Shiga toxin-producing Escherichia coli in FSIS regulatory pork samples

- (Abstract Only)Bosilevac, J.M., Scott, M., Tankson, J. 2021. Assessing Shiga toxin-producing Escherichia coli in FSIS regulatory pork samples [Abstract]. Journal of Food Protection. 84(Suppl A):56.Use of new markers for precise detection of pathogenic Shiga toxin-producing Escherichia coli

- (Abstract Only)Bosilevac, J.M., Bugarel, M., Machado, M., Carrico, J.A., Chablain, P., Briese, D., Murray, J., Bailey, J., Dutta, V. 2021. Use of new markers for precise detection of pathogenic Shiga toxin-producing Escherichia coli. Journal of Food Protection. 84(Suppl A): P. 113-114.Isolation and characterization of an Enteropathogen growth stimulating factor from bovine tissues

- (Abstract Only)Olstein, A., Bosilevac, J.M., Richardson, A. 2021. Isolation and characterization of an Enteropathogen growth stimulating factor from bovine tissues. [Abstract]. Journal of Food Protection. 84(Suppl A): P. 207.High-resolution melt assay for detection of virulent lineages of Shiga toxin-producing Escherichia coli O26 and O111

- (Abstract Only)Velez, F.J., Bosilevac, J.M., Singh, P. 2021. High-resolution melt assay for detection of virulent lineages of Shiga toxin-producing Escherichia coli O26 and O111. [Abstract]. Journal of Food Protection. 84(Suppl A): P. 151-152.Shiga Toxin-producing Escherichia coli (STEC) in the raw pork production chain: A cause for concern?

- (Abstract Only)Haque, M., Bosilevac, J.M., Chaves, B. 2021. Shiga Toxin-producing Escherichia coli (STEC) in the raw pork production chain: A cause for concern?.[Abstract] Journal of Food Protection. 84(Suppl A): P. 192.Ensuring Escherichia coli possessing colibactin genes (clb) linked to colorectal cancer do not become a food safety problem for beef

- (Abstract Only)Guragain, M., Schmidt, J.W., Kalchayanand, N., Bosilevac, J.M. 2023. Ensuring Escherichia coli possessing colibactin genes (clb) linked to colorectal cancer do not become a food safety problem for beef. Journal of Food Protection. 84(Suppl A): P. 134.Validation of high-resolution melting assays for the detection of virulent strains of Escherichia coli O26 and O111 in beef and pork enrichment broths
                                                        

- (Peer Reviewed Journal)Velez, F.J., Bosilevac, J.M., Singh, P. 2021. Validation of high-resolution melting assays for the detection of virulent strains of Escherichia coli O26 and O111 in beef and pork enrichment broths. Food Control. 128. Article 108123. https://doi.org/10.1016/j.foodcont.2021.108123.Locus of Heat Resistance (LHR) in meat-borne Escherichia coli: Screening and genetic characterization
                                                        

- (Peer Reviewed Journal)Guragain, M., Brichta-Harhay, D.M., Bono, J.L., Bosilevac, J.M. 2021. Locus of Heat Resistance (LHR) in meat-borne Escherichia coli: Screening and genetic characterization. Applied and Environmental Microbiology. 87:e02343-20. https://doi.org/10.1128/AEM.02343-20.Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States
                                                        

- (Peer Reviewed Journal)Nastasijevic, I., Schmidt, J.W., Boskovic, M., Glisic, M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Bosilevac, J.M. 2020. Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States. Applied and Environmental Microbiology. 87(1):e01711-20. https://doi.org/10.1128/AEM.01711-20.Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization
                                                        

- (Peer Reviewed Journal)Dass, S.C., Bosilevac, J.M., Weinroth, M., Elowsky, C.G., Zhou, Y., Anandappa, A., Wang, R. 2020. Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization. NPJ Science of Food. 4:16. https://doi.org/10.1038/s41538-020-00076-x.Rapid estimation of Salmonella enterica contamination level in ground beef – application of the time-to-positivity method using a combination of molecular detection and direct plating
                                                        

- (Peer Reviewed Journal)Harhay, D.M., Weinroth, M.D., Bono, J.L., Harhay, G.P., Bosilevac, J.M. 2021. Rapid estimation of Salmonella enterica contamination level in ground beef – application of the time-to-positivity method using a combination of molecular detection and direct plating. Food Microbiology. 93. Article 103615. https://doi.org/10.1016/j.fm.2020.103615.Screening and characterization of genetic locus of heat resistance (LHR) in meat-borne Escherchia coli

- (Abstract Only)Guragain, M., Kalchayanand, N., Wang, R., Bosilevac, J.M. 2020. Screening and characterization of genetic locus of heat resistance (LHR) in meat-borne Escherchia coli [abstract]. American Society for Microbiology Microbe Conference. Available:  https://www.abstractsonline.com/pp8/#!/9103/presentation/7252.High-resolution melting real-time PCR assays for detection of Escherichia coli O26 and O111 strains possessing Shiga toxin genes
                                                        

- (Peer Reviewed Journal)Singh, P., Cubillos, G., Kirshteyn, G., Bosilevac, J.M. 2020. High-resolution melting real-time PCR assays for detection of Escherichia coli O26 and O111 strains possessing Shiga toxin genes. LWT - Food Science and Technology. 131:109785. https://doi.org/10.1016/j.lwt.2020.109785.Evaluation of the post-enrichment process times for commercial E. coli O157:H7 molecular detection systems

- (Abstract Only)Bosilevac, J.M., Ahmed, M., Dutta, V. 2020. Evaluation of the post-enrichment process times for commercial E. coli O157:H7 molecular detection systems. [Abstract]. International Association for Food Protection Conference, Cleveland, Ohio, August 2-5, 2020, Virtual. Journal of Food Protection. 83(Supplement A):P1-98.An intervention applied to meat trimmings before grinding addressed a recurring spoilage and shelf-life problem in a commercial setting

- (Abstract Only)Bosilevac, J.M., Feinberg, J., Maehler, R., Ogunrinola, Y. 2020. An intervention applied to meat trimmings before grinding addressed a recurring spoilage and shelf-life problem in a commercial setting. [Abstract]. International Association for Food Protection Conference, Cleveland Ohio, August 2-5, 2020, Virtual. Journal of Food Protection. 83(Supplement A):P1-178.Evaluation of GENE-UP® new markers EHEC for detection of Shiga Toxin-producing Escherichia coli in MicroTally sheets collected from beef carcasses

- (Abstract Only)Liu, T., Bosilevac, J.M., Wheeler, T.L., Arthur, T.M., Jia, M., Geornaras, I., Dutta, V., Belk, K.E., Yang, H. 2020. Evaluation of GENE-UP® new markers EHEC for detection of Shiga Toxin-producing Escherichia coli in MicroTally sheets collected from beef carcasses. [Abstract]. International Association for Food Protection, Cleveland Ohio, August 2-5, 2020, Virtual. Journal of Food Protection. 83(Supplement 1):P2-70.Attachment of GFP-producing Escherichia coli O103 on beef tissues over time

- (Abstract Only)Brethour, B., Bosilevac, J.M., Maher, J., Stull, K.J., Gragg, S. 2020. Attachment of GFP-producing Escherichia coli O103 on beef tissues over time. [Abtract]. International Association for Food Protection Conference, Cleveland Ohio, August 2-5, 2020, Virtual. Journal of Food Protection. 83(Supplement A):P2-92.High prevalence of extremely heat-resistant Escherichia coli in finished beef products

- (Abstract Only)Guragain, M., Schmidt, J., Bosilevac, J.M. 2020. High prevalence of extremely heat-resistant Escherichia coli in finished beef products. [Abstract] International Association for Food Protection Proceedings, August 2-5, 2020, San Antonio, Texas [Virtual].  Journal of Food Protection. 83(Supplement A):P2-99.Evaluation of UVC radiation and a UVC-ozone combination as fresh beef interventions against Shiga Toxin-producing Escherichia coli, Salmonella, and Listeria monocytogenes and their effects on beef quality
                                                        

- (Peer Reviewed Journal)Kalchayanand, N., Bosilevac, J.M., King, D.A., Wheeler, T.L. 2020. Evaluation of UVC radiation and a UVC-ozone combination as fresh beef interventions against Shiga Toxin-producing Escherichia coli, Salmonella, and Listeria monocytogenes and their effects on beef quality. Journal of Food Protection. 83(9):1520-1529. https://doi.org/10.4315/JFP-19-473.Prevalence of extreme heat-resistant gram-negative bacteria carried by U.S. cattle at harvest
                                                        

- (Peer Reviewed Journal)Guragain, M., Smith, G.E., King, D.A., Bosilevac, J.M. 2020. Prevalence of extreme heat-resistant gram-negative bacteria carried by U.S. cattle at harvest. Journal of Food Protection. 83(8):1438-1443. https://doi.org/10.4315/JFP-20-103.Genomic-based identification of environmental and clinical Listeria monocytogenes strains associated with an abortion outbreak in beef heifers
                                                        

- (Peer Reviewed Journal)Whitman, K.J., Bono, J.L., Clawson, M.L., Loy, J.D., Bosilevac, J.M., Arthur, T.M., Ondrak, J.D. 2020. Genomic-based identification of environmental and clinical Listeria monocytogenes strains associated with an abortion outbreak in beef heifers. BMC Veterinary Research. 16:70. https://doi.org/10.1186/s12917-020-2276-z.Food service pork chops from three U.S. regions harbor similar levels of antimicrobial resistance regardless of antibiotic use claims
                                                        

- (Peer Reviewed Journal)Vikram, A., Miller, E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Schmidt, J.W. 2019. Food service pork chops from three U.S. regions harbor similar levels of antimicrobial resistance regardless of antibiotic use claims. Journal of Food Protection. 82(10):1667-1676. https://doi.org/10.4315/0362-028x.jfp-19-139.Prevalence and characterization of Salmonella present during veal harvest
                                                        

- (Peer Reviewed Journal)Bosilevac, J.M., Zhilyaev, S., Wang, R., Luedtke, B., Wheeler, T.L., Koohmaraie, M. 2019. Prevalence and characterization of Salmonella present during veal harvest. Journal of Food Protection. 82(5):775-784. https://doi.org/10.4315/0362-028X.JFP-18-478.Comparative performance evaluation of real-time PCR and dual-labeled fluorescence resonance energy transfer probe-based melt peak analysis for the detection of Escherichia coli O157:H7 in beef products
                                                        

- (Peer Reviewed Journal)Bosilevac, J.M., Dwivedi, H.P., Chablain, P., Ullery, M., Bailey, J.S., Dutta, V. 2019. Comparative performance evaluation of real-time PCR and dual labeled fluorescence resonance energy transfer probe-based melt peak analysis for the detection of Escherichia coli O157:H7 in beef products. Journal of Food Protection. 82(3):507-512. https://doi.org/10.4315/0362-028X.JFP-18-366.Detection of Shiga toxin producing Escherichia coli, stx1, stx2 and Salmonella by two high resolution melt curve multiplex real-time PCR
                                                        

- (Peer Reviewed Journal)Singh, P., Liu, Y., Bosilevac, J.M., Mustapha, A. 2018. Detection of Shiga toxin producing Escherichia coli, stx1, stx2 and Salmonella by two high resolution melt curve multiplex real-time PCR. Food Control. 96:251-259. https://doi.org/10.1016/j.foodcont.2018.09.024.Similar levels of antimicrobial resistance in U.S. food service ground beef products with and without a "Raised Without Antibiotics" claim
                                                        

- (Peer Reviewed Journal)Vikram, A., Miller, E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Schmidt, J.W. 2018. Similar levels of antimicrobial resistance in U.S. food service ground beef products with and without a "Raised Without Antibiotics" claim. Journal of Food Protection. 81(12):2007-2018. https://doi.org/10.4315/0362-028X.JFP-18-299.Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association
                                                        

- (Peer Reviewed Journal)Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovina, M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2018. Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association. Microbial Genomics. 4:1-17. https://doi.org/10.1099/mgen.0.000202.Extremely heat-resistant Escherichia coli among cattle and beef

- (Abstract Only)Bosilevac, J.M. 2018. Extremely heat-resistant Escherichia coli among cattle and beef. Journal of Food Protection. 81 (Supplement A):P3-13.A Novel selective medium for simultaneous enrichment of Shiga Toxin-producing Escherichia coli and Salmonella in ground beef
                                                        

- (Peer Reviewed Journal)Eggers, J., Feirtag, J.M., Olstein, A.D., Bosilevac, J.M. 2018. A Novel selective medium for simultaneous enrichment of Shiga Toxin-producing Escherichia coli and Salmonella in ground beef. Journal of Food Protection. 81(8):1252-1257. https://doi.org/10.4315/0362-028X.JFP-17-520.Surface pH of fresh beef as a parameter to validate effectiveness of lactic acid treatment against Escherichia O157:H7 and Salmonella
                                                        

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Shackelford, S.D., Brown, T., Wheeler, T.L. 2018. Surface pH of fresh beef as a parameter to validate effectiveness of lactic acid treatment against Escherichia O157:H7 and Salmonella. Journal of Food Protection. 81(7):1126-1133. https://doi.org/10.4315/0362-028X.JFP-17-469.Salmonella in peripheral lymph nodes of healthy cattle at slaughter

- (Peer Reviewed Journal)Webb, H.E., Harhay, D.M., Brashers, M.M., Nightengale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Granier, S.A., Brown, T.R., Edrington, T.S., Shackelford, S.D., Wheeler, T.L., Loneragan, G.H. 2017. Salmonella in peripheral lymph nodes of healthy cattle at slaughter. Frontiers in Microbiology. 8:2214. https://doi.org/10.3389/fmicb.2017.02214.Impact of "raised without antibiotics" beef cattle production practices on occurrences of antimicrobial resistance

- (Peer Reviewed Journal)Vikram, A., Rovira, P., Agga, G.E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Morley, P., Belk, K., Schmidt, J.W. 2017. Impact of "raised without antibiotics" beef cattle production practices on occurrences of antimicrobial resistance. Applied and Environmental Microbiology. 83:e01682-17. https://doi.org/10.1128/AEM.01682-17.Biofilm formation, antimicrobial resistance, and sanitizer tolerance of Salmonella enterica strains isolated from beef trim
                                                        

- (Peer Reviewed Journal)Wang, R., Schmidt, J.W., Harhay, D.M., Bosilevac, J.M., King, D.A., Arthur, T.M. 2017. Biofilm formation, antimicrobial resistance, and sanitizer tolerance of Salmonella enterica strains isolated from beef trim. Foodborne Pathogens and Disease. 14(12):687-695. https://doi.org/10.1089/fpd.2017.2319.Shiga toxin-producing serogroup O91 Escherichia coli strains isolated from food and environmental samples
                                                        

- (Peer Reviewed Journal)Feng, P.C.H., Delannoy, S., Lacher, D.W., Bosilevac, J.M., Fach, P., Beutin, L. 2017. Shiga toxin-producing serogroup O91 Escherichia coli strains isolated from food and environmental samples. Applied and Environmental Microbiology. 83(18):e01231-17. https://doi.org/10.1128/AEM.01231-17.Rapid detection and classification of Salmonella enterica shedding in feedlot cattle utilizing Roka Bioscience Atlas Salmonella detection assay for the analysis of rectoanal mucosal swabs
                                                        

- (Peer Reviewed Journal)Chaney, W.E, Agga, G.E., Nguyen, S.V., Arthur, T.M., Bosilevac, J.M., Dreyling, E., Rishi, A., Brichta-Harhay, D. 2017. Rapid detection and classification of Salmonella enterica shedding in feedlot cattle utilizing Roka Bioscience Atlas Salmonella detection assay for the analysis of rectoanal mucosal swabs. Journal of Food Protection. 80(10):1760-1767. https://doi.org/10.4315/0362-028X.JFP-17-124.Tracking contamination through ground beef production and identifying points of recontamination using a novel green fluorescent protein (GFP) expressing, E. coli O103, non-pathogenic surrogate

- (Abstract Only)Bosilevac, J.M., Luedtke, B., Wang, R., Ogunrinola, Y. 2017. Tracking contamination through ground beef production and identifying points of recontamination using a novel green fluorescent protein (GFP) expressing, E. coli O103, non-pathogenic surrogate. [Abstract]. International Association for Food Protection Proceedings. P1-136. Available at:  https://iafp.confex.com/iafp/2017/webprogram/authork.html.A comparative evaluation of the GENE-up assay for the detection of Escherichia coli O157:H7

- (Abstract Only)Dutta, V., Bosilevac, J.M., Dwivedi, H.P., Mills, J.C., Chablain, P., Bailey, S.J. 2017. [Abstract]. A comparative evaluation of the GENE-up assay for the detection of Escherichia coli O157:H7. International Association for Food Protection Proceedings.  P2-197. Available at: https://iafp.confex.com/iafp/2017/webprogram/authork.html.Outcomes of using wet pooling to detect STEC and Salmonella

- (Abstract Only)Bosilevac, J.M. 2017. Outcomes of using wet pooling to detect STEC and Salmonella. Meeting Abstract. [Abstract]. Beef Industry Safety Summit, February 28-March 2, 2017, Houston, Texas. Available:http://bifsco.org/search.aspx?qal=2017 abstracts.Impact of antimicrobial use during beef production on fecal occurrence of antimicrobial resistance

- (Abstract Only)Vikram, A., Rovira, P., Agga, G.E., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Morley, P., Belk, K., Schmidt, J.W. 2017. Impact of antimicrobial use during beef production on fecal occurrence of antimicrobial resistance. Meeting Abstract. [Abstract]. Beef Industry Safety Summit, February 28-March 2, 2017, Houston, Texas. Available:http://bifsco.org/search.aspx?qal=2017 abstracts.Evaluation of rectoanal mucosal swab sampling for molecular detection of Enterohemorrhagic Escherichia coli in beef cattle
                                                        

- (Peer Reviewed Journal)Agga, G.E., Arthur, T.M., Hinkley, S., Bosilevac, J.M. 2017. Evaluation of rectoanal mucosal swab sampling for molecular detection of Enterohemorrhagic Escherichia coli in beef cattle. Journal of Food Protection. 80(4):661-667.  doi:10.4315/0362-028X.JFP-16-435.Methods for detecting pathogens in the beef food chain: detecting particular pathogens

- (Book / Chapter)Fratamico, P.M., Bosilevac, J.M., Schmidt, J.W. 2017. Methods for detecting pathogens in the beef food chain: detecting particular pathogens. In: Acuff, G., Dickson, J. Ensuring safety and quality in the production of beef. Volume 1: Safety. Cambridge, UK: Burleigh Dobbs Science. p.59-72.Characterization and virulence potential of serogroup O113 Shiga toxin-producing Escherichia coli strains isolated from beef and cattle in the United States

- (Peer Reviewed Journal)Feng, P., Delannoy, S., Lacher, D., Bosilevac, J.M., Fach, P. 2017. Characterization and virulence potential of serogroup O113 Shiga toxin-producing Escherichia coli strains isolated from beef and cattle in the United States. Journal of Food Protection. 80(3):383-391.  doi:10.4315/0362-028X.JFP-16-325.Complete, closed genome sequences of 10 Salmonella enterica subsp.
enterica serovar Typhimurium strains isolated from human and
bovine sources
                                                        

- (Peer Reviewed Journal)Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete, closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Typhimurium strains isolated from human and bovine sources. Genome Announcements. 4(6):e01212-16. doi:10.1128/genomeA.01212-16.Characterization of enterohemorrhagic Escherichia coli on veal hides and carcasses
                                                        

- (Peer Reviewed Journal)Bosilevac, J.M., Wang, R., Luedtke, B.E., Hinkley, S., Wheeler, T.L., Koohmaraie, M. 2017. Characterization of enterohemorrhagic Escherichia coli on veal hides and carcasses. Journal of Food Protection. 80(1):136-145. doi:10.4315/0362.028X.JFP-16-247.Methods for detecting pathogens in the beef food chain: an overview

- (Book / Chapter)Fratamico, P.M., Bosilevac, J.M., Schmidt, J.W. 2017. Methods for detecting pathogens in the beef food chain: an overview. In: Acuff, G., Dickson, J. Ensuring safety and quality in the production of beef. Volume 1: Safety. Cambridge, UK: Burleigh Dobbs Science. p.35-51.Escherichia coli O157:H7 strains isolated from High-Event Period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number
                                                        

- (Peer Reviewed Journal)Wang, R., Luedtke, B.E., Bosilevac, J.M., Schmidt, J.W., Kalchayanand, N., Arthur, T.M. 2016. Escherichia coli O157:H7 strains isolated from High Event Period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number. Journal of Food Protection. 79(11):1875-1883.  doi:10.4315/0362-028X.JFP-16-113.Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources
                                                        

- (Peer Reviewed Journal)Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources. Genome Announcements. Vol. 4(3): e00447-16. doi: 10.1128/genomeA.00447-16.Contamination revealed by indicator microorganism levels during veal processing
                                                        

- (Peer Reviewed Journal)Bosilevac, J.M., Wang, R., Luedtke, B.E., Wheeler, T.L., Koohmaraie, M. 2016. Contamination revealed by indicator microorganism levels during veal processing. Journal of Food Protection. 79(8):1341-1347. doi:10.4315/0362-028X.JFP-15-572.Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination

- (Abstract Only)Bosilevac, J.M., Luedtke, B.E., Wang, R., Ogunrinola, Y. 2016. Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination.[abstract] Beef Industry Safety Summit, March 1-3, 2016, Austin, Texas. Available:  http://www.bifsco.org/2016researchsessionabstracts.aspx.Effect of direct-fed microbial dosage on the fecal concentrations of enterohemorrhagic Escherichia coli in feedlot cattle
                                                        

- (Peer Reviewed Journal)Luedtke, B.E., Bosilevac, J.M., Harhay, D.M., Arthur, T.M. 2016. Effect of direct-fed microbial dosage on the fecal concentrations of enterohemorrhagic Escherichia coli in feedlot cattle. Foodborne Pathogens and Disease. 13(4):190-195. doi:10.1089/fpd.2015.2063Prevalence and level of enterohemorrhagic Escherichia coli in culled dairy cows at harvest

- (Peer Reviewed Journal)Stromberg, Z.R., Lewis, G.L., Aly, S.S., Lehenbauer, T.W., Bosilevac, J.M., Cernicchiaro, N., Moxley, R.A. 2016. Prevalence and level of enterohemorrhagic Escherichia coli in culled dairy cows at harvest. Journal of Food Protection. 79(3):421-431.Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses
                                                        

- (Peer Reviewed Journal)Luedtke, B.E., Bosilevac, J.M. 2015. Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses. Front Microbiol. 6:1062. doi:10.3389/frmicb.2015.01062.Genetic diversity and virulence potential of Shiga-toxigenic Escherichia coli strains of O113 serogroup isolated from ground beef in the U.S.

- (Abstract Only)Feng, P., Delannoy, S., Lacher, D., Bosilevac, J.M., Fach, P. 2015. Genetic diversity and virulence potential of Shiga-toxigenic Escherichia coli strains of O113 serogroup isolated from ground beef in the U.S.[Abstract]. Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections (VTEC) 2015 Conference. Section C, No 4.Variations in the fecal occurrences of antimicrobial-resistant bacteria are greater between seasons than between "raised without antibiotics" and "conventional" cattle production systems

- (Abstract Only)Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Wheeler, T.L., Arthur, T.M. 2015. Variations in the fecal occurrences of antimicrobial-resistant bacteria are greater between seasons than between "raised without antibiotics" and "conventional" cattle production systems.[Abstract] 4th American Society for Microbiology Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens. S7:4,p.102.Effects of organic acid-surfactant mixtures on levels of bacteria and beef quality traits

- (Abstract Only)Bosilevac, J.M., Kalchayanand, N., King, D.A. 2015. Effects of organic acid-surfactant mixtures on levels of bacteria and beef quality traits. [Abstract] International Association for Food Protection Proceedings. Volume 78 (Supplement A): Page 273. (P3-150).Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination

- (Abstract Only)Wang, R., Luedtke, B.E., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Arthur, T.M. 2015. Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination. [Abstract] International Association for Food Protection Proceedings. Volume 78 (Supplement A): page 64. (T10-211).Prevalence and comparison of detection methods of Enterohemorrhagic Escherichia coli in culled dairy cows

- (Abstract Only)Stromberg, Z.R., Lewis, G.L., Aly, S.S., Lehenbauer, T.W., Bosilevac, J.M., Cernicchiaro, N., Moxley, R.A. 2015. Prevalence and comparison of detection methods of Enterohemorrhagic Escherichia coli in culled dairy cows. [Abstract] International Association for Food Protection Proceedings. Volume78 (Supplement A): page 294. (P3-219).Direct rapid detection of beef lymph nodes containing high levels of Salmonella

- (Abstract Only)Bosilevac, J.M., Arthur, T.M. 2015. Direct rapid detection of beef lymph nodes containing high levels of Salmonella [abstract]. Beef Industry Safety Summit. http://www.bifsco.org/2015researchsessionabstracts.aspxEfficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli (STEC) and Salmonella inoculated onto fresh beef
                                                        

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2015. Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli and Salmonella inoculated onto fresh beef. Journal of Food Protection. 78(3):503-510.Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wang, R., Wheeler, T.L., Arthur, T.M. 2015. Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum. Applied and Environmental Microbiology. 81(2):713-725.Distribution of Escherichia coli passaged through processing equipment during ground beef production using inoculated trimmings

- (Peer Reviewed Journal)Koohmaraie, M., De La Zerda, M., Bosilevac, J.M., Ali Mohseni, M., Samadpour, M. 2015. Distribution of Escherichia coli passage through processing equipment during ground beef production using inoculated trimmings. Journal of Food Protection. 78(2):273-280.Prevalence of Escherichia coli 0157:H7 and Salmonella in camel, cattle, goat and sheep harvested for meat in Riyadh
                                                        

- (Peer Reviewed Journal)Bosilevac, J.M., Gassem, M.A., Al Sheddy, I.A., Almaiman, S.A., Al-Mohizea, I., Alowaimer, A., Koohmaraie, M. 2015. Prevalence of Escherichia coli 0157:H7 and Salmonella in camel, cattle, goat and sheep harvested for meat in Riyadh. Journal of Food Protection. 78(1):89-96.Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces
                                                        

- (Peer Reviewed Journal)Luedtke, B.E., Bono, J.L., Bosilevac, J.M. 2014. Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces. Journal of Microbiological Methods. 105:72-79.Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination
                                                        

- (Peer Reviewed Journal)Wang, R., Kalchayanand, N., King, D.A., Luedtke, B.E., Bosilevac, J.M., Arthur, T.M. 2014. Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination. Journal of Food Protection. 77(11):1982-1987.Pre- and post-harvest interventions to reduce pathogen contamination in the U.S. beef industry

- (Proceedings)Wheeler, T.L., Kalchayanand, N., Bosilevac, J.M., 2014. Pre- and post-harvest interventions to reduce pathogen contamination in the U.S. beef industry. In:  Proceedings of the 60th International Congress of Meat Science and technology, August 17-18, 2014, Punta Del Este, Uruguay. Journal of Meat Science. 98:372-382.Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes

- (Abstract Only)Harhay, D.M., Loneragan, G.H., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Wheeler, T.L., Kolath, B., Theunick, D., Riemann, M., Schaefer, D., Brown, T. 2014. Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes. [Abstract]. International Association for Food Protection. Supp. A(77):211-212. P3-65.Evaluation of different immuno-magnetic beads and selective plating media for the confirmation of Shiga toxin-producing Escherichia coli detected by molecular methods in beef trim enrichment broths

- (Abstract Only)Bosilevac, J.M., Luedtke, B.E., Rovira, P. 2014. Evaluation of different immuno-magnetic beads and selective plating media for the confirmation of Shiga toxin-producing Escherichia coli detected by molecular methods in beef trim enrichment broths. [Abstract]. International Association for Food Protection. Supp. A(77):81. P1-21.Examination of predictors of Salmonella enterica contamination in cattle feedlot environments

- (Abstract Only)Harhay, D.M., Arthur, T.M., Schmidt, J.W., Wang, R., Kalchayanand, N., Bosilevac, J.M., Edrington, T.S., Loneragan, G., Wheeler, T.L. 2014. Examination of predictors of Salmonella enterica contamination in cattle feedlot environments. [Abstract] Beef Industray Safety Summit. Available:  www.bifsco.org/attendees.aspx.Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the US

- (Abstract Only)Bosilevac, J.M., Luedtke, B.E., Arthur, T.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L. 2014. Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the U.S. [abstract] Beef Industry Safety Summit.  Available:  www.bifsco.org/attendees.aspx.Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides
                                                        

- (Peer Reviewed Journal)Wang, R., Koohmaraie, M., Wheeler, T.L., Bosilevac, J.M. 2014. Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides. Journal of Food Protection. 77(5):745-751.Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat
                                                        

- (Peer Reviewed Journal)Schmidt, J.W., Bosilevac, J.M., Kalchayanand, N., Wang, R., Wheeler, T.L., Koohmaraie, M. 2014. Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat. Journal of Food Protection. 77(4):538-548.Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions
                                                        

- (Peer Reviewed Journal)Wang, R., King, D.A., Koohmaraie, M., Bosilevac, J.M. 2013. Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions. Journal of Food Protection. 76(12):2069-2073.Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7

- (Abstract Only)Wang, R., Kalchayanand, N., Bosilevac, J.M., Arthur, T.M. 2013. Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7. International Association for Food Protection Proceedings. [abstract] P1-48 Page No.48.Ultraviolet light (UV) and UV-ozone interventions reduce shiga toxin-producing Escherichia coli (STEC) on contaminated fresh beef

- (Abstract Only)Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2013. Ultraviolet light (UV) and UV-ozone interventions reduce shiga toxin-producing Escherichia coli (STEC) on contaminated fresh beef. American Society for Microbiology General Meeting. Poster No. 1596 p.166.Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest

- (Peer Reviewed Journal)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.Salmonella virulence, genomics and interactions with the immune system

- (Proceedings)Harhay, D.M., Smith, T.P., Harhay, G.P., Bono, J.L., Bosilevac, J.M. 2013. Salmonella virulence, genomics and interactions with the immune system. Meeting Abstract. The Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas, FLASHDRIVE.Salmonella in lymph nodes of cull and fed cattle at harvest

- (Abstract Only)Webb, H.E., Harhay, D.M., Gragg, S.E., Brashears, M.M., Nightingale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Lonergan, G.H. 2013. Salmonella in lymph nodes of cull and fed cattle at harvest. IN:  Proceedings of The Beef Industry Summit 2013, March 13-15, 2013, Dallas Texas, 2013 FLASHDRIVE.Comparison of non-O157 Shiga toxin-producing E. coli detection systems

- (Proceedings)Bosilevac, J.M. 2013. Comparison of non-O157 Shiga toxin-producing E. coli detection systems.Proceedings of the Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas. 2013 FLASHDRIVE.Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest

- (Peer Reviewed Journal)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10(4):368-374.Isolation and characterization of Clostridium difficile associated with beef cattle and commercially produced ground beef

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2013. Isolation and characterization of Clostridium difficile associated with beef cattle and commercially produced ground beef. Journal of Food Protection. 76(2):256-264.The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests

- (Peer Reviewed Journal)Wang, R., Schmidt, J.W., Arthur, T.M., Bosilevac, J.M. 2013. The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests. Food Microbiology. 33: 205-212.Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26,O45,O103,O111,O121, and O145 from fresh beef and cattle feces

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wells, J., Wheeler, T.L. 2013. Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26,O45,O103,O111,O121, and O145 from fresh beef and cattle feces. Journal of Food Protection. 76(2):192-199.Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three U.S. commercial beef processing plants

- (Peer Reviewed Journal)Schmidt, J.W., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L. 2012. Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three U. S. commercial beef processing plants. Journal of Food Protection. 75(12):2213-2218.Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups

- (Peer Reviewed Journal)Bosilevac, J.M., Koohmaraie, M. 2012. Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups. Applied and Environmental Microbiology. 78(19):7152-7155.Resistance of various shiga toxin-producing Escherichia coli to electrolyzed oxidizing water

- (Peer Reviewed Journal)Jadeja, R., Hung, Y., Bosilevac, J.M. 2013. Resistance of various shiga toxin-producing Escherichia coli to electrolyzed oxidizing water. Food Control. 30(2):580-584.Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains

- (Peer Reviewed Journal)SWang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.Detection methods and intervention strategies for shiga toxin-producing E. coli in beef

- (Abstract Only)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Detection methods and intervention strategies for shiga toxin-producing E. coli in beef [abstract]. Annual Meeting of the Institute of Food Technologists. 061-01:52.Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2012. Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. Journal of Food Protection. 75(7):1207-1212.Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef

- (Abstract Only)Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2012. Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef.[Abstract] Annual Meeting of the Institute of Food Technologists.June 25-28, 2012, Las Vegas, Nevada. Abstract 035-71:25.Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli isolated from feedlot and cull dairy and beef herd cattle

- (Abstract Only)Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants

- (Peer Reviewed Journal)Schmidt, J.W., Harhay, D.M., Kalchayanand, N., Bosilevac, J.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2012. Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants. Applied and Environmental Microbiology. 78(8):2716-2726.Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica

- (Peer Reviewed Journal)Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Loneragan, G.H., Wheeler, T.L. 2012. Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica. Journal of Food Protection. 75(5):854-858.Salmonella in lymph nodes of cattle presented for harvest

- (Abstract Only)Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois.  p. 149.Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides

- (Peer Reviewed Journal)Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides. Journal of Food Protection. 74(10):1611-1617.Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions

- (Proceedings)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions. Proceedings of American Meat Science Association 64th Annual Reciprocal Meat Conference, June 19-22, 2011, Kansas State University, Manhattan Kansas. p.1-9.Reduction in dietary wet distillers grains alters Escherichia coli 0157:H7 in feces of cattle

- (Abstract Only)Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Reduction in dietary wet distillers grains alters Escherichia coli 0157:H7 in feces of cattle. In: Proceedings of the 2011 Congress on Gastrointestinal Function, April 18-20, 2011, Chicago, Illinois.Survival of Escherichia coli O157:H7 on cattle hides

- (Peer Reviewed Journal)Arthur, T.M., Nou, X., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L., Koohmaraie, M. 2011. Survival of Escherichia coli O157:H7 on cattle hides. Applied and Environmental Microbiology. 77(9):3002-3008.Salmonella in lymph nodes of cattle presented for harvest

- (Abstract Only)Gragg, S.E., Loneragan, G.H., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brashears, M.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. International Association for Food Protection.  p. 19.  Abstract No. P1-62.Sensitivity of Mycobacterium bovis to common beef processing interventions

- (Abstract Only)Bosilevac, J.M., Iwen, P.C. 2011. Sensitivity of Mycobacterium bovis to common beef processing interventions [abstract]. International Association for Food Protection, Milwaukee, WI. July 31 - August 3, 2011. Poster Abstract P2-42, p. 65.Towards an understanding of how Salmonella potentially evades beef carcass processing interventions

- (Abstract Only)Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.Sensitivity of Mycobacterium bovis to common beef processing interventions

- (Abstract Only)Bosilevac, J.M., Iwen, P.C. 2011. Sensitivity of Mycobacterium bovis to common beef processing interventions [abstract]. 2011 Beef Safety Summit, Dallas, TX. March 2-4, 2011. 2011 Beef Industry Safety Summit Research Update. Abstract No. 8.The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7

- (Abstract Only)Bosilevac, J.M., Schmidt, J.W., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2011. The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7 [abstract]. 2011 Beef Industry Safety Summit, Dallas, TX. March 2-4, 2011. 2011 Beef Industry Safety Summit Research Update. Abstract No. 1.Prevalence and characterization of non-O157 Shiga toxin producing Escherichia coli isolates from commercial ground beef in the United States

- (Peer Reviewed Journal)Bosilevac, J.M., Koohmaraie, M. 2011. Prevalence and characterization of non-O157 Shiga toxin producing Escherichia coli isolates from commercial ground beef in the United States. Applied and Environmental Microbiology. 77(6):2103-2112.Diversity of multidrug-resistant Salmonella enterica strains associated with cattle at harvest in the United States

- (Peer Reviewed Journal)Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2011. Diversity of multidrug-resistant Salmonella enterica strains associated with cattle at harvest in the United States. Applied and Environmental Microbiology. 77(5):1783-1796.Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods

- (Peer Reviewed Journal)Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods. Journal of Food Protection. 73:2180-2188.Diversity of Multi-drug Resistant Salmonella enterica Associated with Cull Cattle at Harvest in the United States

- (Abstract Only)Harhay, D.M., Poole, T.L., Durso, L.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Diversity of Multi-drug Resistant Salmonella enterica Associated with Cull Cattle at Harvest in the United States [abstract]. American Society for Microbiology. p. 25, Presentation No. S4:1 and p. 111, Poster No. B176.Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination

- (Proceedings)Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination. Meat Science. 86:32-37. Special Issue: 56th International Congress of Meat Science and Technology (56th ICoMST) August 15-20, 2010, Jeju, Korea. (Proceedings) Available: http://dx.doi.org/10.1016/j.meatsci.2010.04.019Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmarie, M. 2010. Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle. Journal of Food Protection. 73(2):366-371.Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and Role of High-Level Shedders in Hide Contamination

- (Peer Reviewed Journal)Arthur, T.M., Keen, J.E., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Nou, X., Koohmaraie, M. 2009. Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and Role of High-Level Shedders in Hide Contamination. Applied and Environmental Microbiology. 75(20):6515-6523.Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles

- (Peer Reviewed Journal)Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Guerini, M.N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles. Journal of Food Protection. 72(8):1624-1633.Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and the Role of High Shedders in Hide Contamination

- (Abstract Only)Arthur, T.M., Keen, J.E., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Nou, X., Koohmaraie, M. 2009. Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and the Role of High Shedders in Hide Contamination [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. 195.Non-O157 Shiga toxin Producing Escherichia coli in United States Beef Production

- (Abstract Only)Bosilevac, J.M., Koohmaraie, M. 2009. Non-O157 Shiga toxin Producing Escherichia coli in United States Beef Production [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. P03.2.2. p 71.Comparison of Colony Hybridization to Phenotype Screening on Washed Sheep's Blood Agar for the Isolation of Shiga toxin Producing Escherichia coli from Complex Matrixes

- (Abstract Only)Bosilevac, J.M., Koohmaraie, M. 2009. Comparison of Colony Hybridization to Phenotype Screening on Washed Sheep's Blood Agar for the Isolation of Shiga toxin Producing Escherichia coli from Complex Matrixes [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. P02.5.3. p. 106.Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States

- (Peer Reviewed Journal)Wells, J., Bosilevac, J.M., Kalchayanand, N., Arthur, T.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States. Journal of Food Protection 72(7):1457-1462.Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles

- (Abstract Only)Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009.  Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles [abstract]. Microbial Ecology 57(3):583-584.Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States

- (Abstract Only)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States [abstract]. Institute of Food Technologists (IFT), Division Food Microbiology, Session 026-15, p. 82.Prevalence and Characterization of Salmonellae in Commercial Ground Beef in the United States

- (Peer Reviewed Journal)Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2009. Prevalence and Characterization of Salmonellae in Commercial Ground Beef in the United States. Applied and Environmental Microbiology. 75(7):1892-1900.Prevalence Rates of Escherichia coli O157:H7 and Salmonella at Different Sampling Sites on Cattle Hides at a Feedlot and Processing Plant

- (Peer Reviewed Journal)Kalchayanand, N., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Guerini, M.N., Wheeler, T.L., Shackelford, S.D., Koohmaraie, M. 2009. Prevalence Rates of Escherichia coli O157:H7 and Salmonella at Different Sampling Sites on Cattle Hides at a Feedlot and Processing Plant. Journal of Food Protection. 72(6):1267-1271.Characterization of Multidrug-resistant Salmonella Isolated from Cull and Fed Cattle at Slaughter in the United States

- (Abstract Only)Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Characterization of Multidrug-resistant Salmonella Isolated from Cull and Fed Cattle at Slaughter in the United States [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7

- (Abstract Only)Bosilevac, J.M., Wells, J., Arthur, T.M., Kalchayanand, N., Shackelford, S.D., Berry, E.D., Bono, J.L., Harhay, D.M., Wheeler, T.L., Koohmaraie, M. 2009. Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day

- (Peer Reviewed Journal)Bosilevac, J.M., Arthur, T.M., Bono, J.L., Brichta-Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day. Journal of Food Protection. 72(6):1272-1278.Effectiveness of 1,3-Dibromo-5,5 dimethylhydantoin on reduction of Escherichia coli O157:H7- and Salmonella-inoculated fresh meat

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Guerini, M.N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Effectiveness of 1,3-Dibromo-5,5 dimethylhydantoin on reduction of Escherichia coli O157:H7- and Salmonella-inoculated fresh meat. Journal of Food Protection 72(1):151-156.Increased Detection of Listeria Species and Listeria Monocytogenes in Raw Beef, Using the Assurance GDS Molecular Detection System with Culture Isolation

- (Peer Reviewed Journal)Bosilevac, J.M., Guerini, M.N., Koohmaraie, M. 2009. Increased Detection of Listeria Species and Listeria Monocytogenes in Raw Beef, Using the Assurance GDS Molecular Detection System with Culture Isolation. Journal of Food Protection. 72(3):674-679.Salmonella and E. coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the U.S.: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods

- (Peer Reviewed Journal)Harhay, D.M., Guerini, M.N., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods. 2008. Applied and Environmental Microbiology 74(20):6289-6297.Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7

- (Peer Reviewed Journal)Arthur, T.M., Kalchayanand, N., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Bono, J.L., Wheeler, T.L., Koohmaraie, M. 2008. Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7. Journal of Food Protection 71(11):2177-2181.Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention. Journal of Food Protection. 71(9):1752-1760.Effects of using reduced volumes of nonselective enrichment medium in methods for the detection of Escherichia coli O157:H7 from raw beef

- (Review Article)Bosilevac, J.M., Koohmaraie, M. 2008. Effects of using reduced volumes of nonselective enrichment medium in methods for the detection of Escherichia coli O157:H7 from raw beef. Journal of Food Protection 71(9):1768-1773.Prevalence and Characterization of Salmonella in Bovine Lymph Nodes Potentially Destined for Use in Ground Beef

- (Peer Reviewed Journal)Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Prevalence and characterization of Salmonella in bovine lymph nodes potentially destined for use in ground beef. Journal of Food Protection 71(8):1685-1688.Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Guerini, M.N., Wheeler, T.L., Koohmaraie, M. 2008. Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing. Journal of Food Protection 71(3):621-624.Listeria prevalence and L. monocytogenes serovar diversity at cull cow/bull processing plants in the United States

- (Peer Reviewed Journal)Guerini, M.N., Harhay, D.M., Shackelford, S.D., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2007. Listeria prevalence and Listeria monocytogenes serovar diversity at cull cow and bull processing plants in the United States. Journal of Food Protection. 70(11):2578-2582.Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America. Journal of Food Protection. 70(7):1622-1626.Microbiological characterization of lamb carcasses at commercial processing plants in the United States

- (Peer Reviewed Journal)Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Microbiological characterization of lamb carcasses at commercial processing plants in the United States. Journal of Food Protection 70(8):1811-1819.Enumeration of Salmonella and Escherichia coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Fecal Samples Using Direct Plating Methods

- (Peer Reviewed Journal)Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2007. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. Journal of Applied Microbiology 103(5):1657-1668.Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter. Journal of Food Protection 70:1076-1079.Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing. Journal of Food Protection 70(2):280-286.MICROBIOLOGICAL CHARACTERIZATION OF IMPORTED AND DOMESTIC BONELESS BEEF TRIM USED FOR GROUND BEEF

- (Peer Reviewed Journal)Bosilevac, J.M., Guerini, M.N., Harhay, D.M., Arthur, T.M., Koohmaraie, M. 2007. Microbiological characterization of imported and domestic boneless beef trim used for ground beef. Journal of Food Protection 70(2):440-449.RAPID ENRICHMENT STRATEGY FOR ISOLATION OF LISTERIA FROM BOVINE HIDE, CARCASS, AND MEAT SAMPLES

- (Peer Reviewed Journal)Guerini, M.N., Bosilevac, J.M., Koohmaraie, M. 2007. Rapid enrichment strategy for isolation of Listeria from bovine hide, carcass, and meat samples. Journal of Food Protection 70(1):53-57.IMPROVEMENT OF IMMUNOMAGNETIC SEPARATION FOR ESCHERICHIA COLI O157:H7 DETECTION BY THE PICKPEN MAGNETIC PARTICLE SEPARATION DEVICE

- (Peer Reviewed Journal)Nou, X., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2006. Improvement of immunomagnetic separation for Escherichia coli O157:H7 detection by the pickpen magnetic particle separation device. Journal of Food Protection 69:2870-2874.TREATMENTS USING HOT WATER INSTEAD OF LACTIC ACID REDUCE LEVELS OF AEROBIC BACTERIA AND ENTEROBACTERIACEAE AND REDUCE THE PREVALENCE OF ESCHERICHIA COLI O157:H7 ON PREEVISCERATION BEEF CARCASSES

- (Peer Reviewed Journal)Bosilevac, J.M., Nou, X., Barkocy-Gallagher, G.A., Arthur, T.M., Koohmaraie, M. 2006. Treatments using hot water instead of lactic acid reduce levels of aerobic bacteria and Enterobacteriaceae and reduce the prevalence of Escherichia coli O157:H7 on preevisceration beef carcasses. Journal of Food Protection. 69(8):1808-1813.METHODS FOR RECOVERING ESCHERICHIA COLI O157:H7 FROM CATTLE FECAL, HIDE, AND CARCASS SAMPLES: SENSITIVITY AND IMPROVEMENTS

- (Peer Reviewed Journal)Barkocy-Gallagher, G.A., Edwards, K.K., Nou, X., Bosilevac, J.M., Arthur, T.M., Shackelford, S.D., Koohmaraie, M. 2005. Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples: Sensitivity and improvements. Journal of Food Protection. 68:2264-2268.EVALUATION OF CULTURE- AND PCR-BASED DETECTION METHODS FOR ESCHERICHIA COLI O157:H7 IN INOCULATED GROUND BEEF

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Evaluation of culture- and PCR-based detection methods for Escherichia coli O157:H7 in inoculated ground beef. Journal of Food Protection. 68:1566-1574.EFFICACY OF OZONATED AND ELECTROLYZED OXIDATIVE WATERS TO DECONTAMINATE HIDES OF CATTLE BEFORE SLAUGHTER

- (Peer Reviewed Journal)Bosilevac, J.M., Shackelford, S.D., Brichta, D.M., Koohmaraie, M. 2005. Efficacy of ozonated and electrolyzed oxidative waters to decontaminate hides of cattle before slaughter. Journal of Food Protection. 68:1393-1398.EFFECTS OF LOW-DOSE, LOW-PENETRATION ELECTRON BEAM IRRADIATION OF CHILLED BEEF CARCASS SURFACE CUTS ON ESCHERICHIA COLI O157:H7 AND MEAT QUALITY

- (Peer Reviewed Journal)Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Effects of low-dose, low-penetration electron beam irradiation of chilled beef carcass surface cuts on Escherichia coli O157:H7 and meat quality. Journal of Food Protection. 68(4):666-672.DEVELOPMENT AND EVALUATION OF AN ON-LINE HIDE DECONTAMINATION PROCEDURE FOR USE IN A COMMERCIAL BEEF PROCESSING PLANT

- (Peer Reviewed Journal)Bosilevac, J.M., Nou, X., Osborn, M.S., Allen, D.M., Koohmaraie, M. 2005. Development and evaluation of an on-line hide decontamination procedure for use in a commercial beef processing plant. Journal of Food Protection. 68(2):265-272.DECREASED DOSAGE OF ACIDIFIED SODIUM CHLORITE REDUCES MICROBIAL CONTAMINATION AND MAINTAINS ORGANOLEPTIC QUALITIES OF GROUND BEEF PRODUCTS

- (Peer Reviewed Journal)Bosilevac, J.M., Shackelford, S.D., Fahle, R., Biela, T., Koohmaraie, M. 2004. Decreased dosage of acidified sodium chlorite reduces microbial contamination and maintains organoleptic qualities of ground beef products. Journal of Food Protection 67:2248-2254.ESCHERICHIA COLI O157 PREVALENCE AND ENUMERATION OF AEROBIC BACTERIA, ENTEROBACTERIACEAE, AND ESCHERICHIA COLI O157 AT VARIOUS STEPS IN COMMERCIAL BEEF PROCESSING PLANTS

- (Peer Reviewed Journal)Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Kent, M.P., Jaroni, D., Pauling, B., Allen, D.M., Koohmaraie, M. 2004. Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriacae, and Escherichia coli O157 at various steps in commercial beef processing plants. Journal of Food Protection. 67(4):658-665.PREVALENCE OF ESCHERICHIA COLI O157 AND LEVELS OF AEROBIC BACTERIA AND ENTEROBACTERIACEAE ARE REDUCED WHEN HIDES ARE WASHED AND TREATED WITH CETYLPYRIDINIUM CHLORIDE AT A COMMERCIAL BEEF PROCESSING PLANT

- (Peer Reviewed Journal)Bosilevac, J.M., Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Prevalence of Escherichia coli O157 and levels of aerobic bacteria and Enterobacteriaceae are reduced when hides are washed and treated with cetylpyridinium chloride at a commercial beef processing plant. Journal of Food Protection. 67(4):646-650.PROTOCOL FOR EVALUATING THE EFFICACY OF CETYLPYRIDINIUM CHLORIDE AS A BEEF HIDE INTERVENTION

- (Peer Reviewed Journal)Bosilevac, J.M., Wheeler, T.L., Rivera-Betancourt, M., Nou, X., Arthur, T.M., Shackelford, S.D., Kent, M.P., Jaroni, D., Osborn, M.S., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Protocol for evaluating the efficacy of cetylpyridinium chloride as a beef hide intervention. Journal of Food Protection. 67(2):303-309.EFFECT OF CHEMICAL DEHAIRING ON THE PREVALENCE OF ESCHERICHIA COLI O157:H7 AND THE LEVELS OF AEROBIC BACTERIA AND ENTEROBACTERIACEAE ON CARCASSES IN A COMMERCIAL BEEF PROCESSING PLANT

- (Peer Reviewed Journal)Nou, X., Rivera-Betancourt, M., Bosilevac, J.M., Wheeler, T.L., Shackelford, S.D., Gwartney, B.L., Reagan, J.O., Koohmaraie, M. 2003. Effect of chemical dehairing on the prevalence of Escherichia coli O157:H7 and the levels of aerobic bacteria and Enterobacteriaceae on carcasses in a commercial beef processing plant. Journal of Food Protection. 66(11):2005-2009.
                    ARS News Articles
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                                                Scientists Scrutinize Role of "Supershedder" Cattle in E. coli O157:H7 Contaminationitem
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                                                Food Safety Study of Beef "Trim" Leads to Ongoing Research Collaboration


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