U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Produce Safety and Microbiology Research: Albany, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Albany, California Western Regional Research Center Produce Safety and Microbiology Research People & Locations







Leslie  Harden

(Les)
                        Produce Safety and Microbiology Research

                        




                        
                        Chemist
                        Phone: (510) 559-5714
                        
                        
                        Fax: (510) 559-5777(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
                            **Publications**
                                                (Clicking on the reprint icon
                                                    Reprint Icon
                                                    will take you to the publication reprint.)Utilising natural diversity of kinases to rationally engineer interactions with the angiosperm immune receptor ZAR1
                                                        

- (Peer Reviewed Journal)Diplock, N., Baudin, M., Harden, L.A., Silva, C.J., Erickson-Beltran, M.L., Hassan, J.A., Lewis, J.D. 2023. Utilising natural diversity of kinases to rationally engineer interactions with the angiosperm immune receptor ZAR1. Plant, Cell & Environment. 46(7):2238-2254. https://doi.org/10.1111/pce.14603.Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit
                                                        

- (Peer Reviewed Journal)Thilmony, R.L., Dasgupta, K., Shao, M., Harris, D., Hartman, J., Harden, L.A., Chan, R., Thomson, J.G. 2022. Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit. Frontiers in Plant Science. 13. Article 945738. https://doi.org/10.3389/fpls.2022.945738.Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on Mung Bean seeds
                                                        

- (Peer Reviewed Journal)Liao, Y., Zhang, Y., Salvador, A., Harden, L.A., Wu, V.C. 2022. Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on Mung Bean seeds. Microbiology Spectrum. 10(1). Article e02220-21. https://doi.org/10.1128/spectrum.02220-21.Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli
                                                        

- (Peer Reviewed Journal)Lavenburg, V., Liao, Y., Salvador, A., Hsu, A.L., Harden, L.A., Wu, V.C. 2020. Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli. Cryobiology. 96:85-91. https://doi.org/10.1016/j.cryobiol.2020.07.012.Phage therapy for controlling Shiga toxin-producing Escherichia coli O103 using lytic bacteriophages

- (Abstract Only)Characterization of a lytic bacteriophage as an antimicrobial agent for biocontrol of Shiga toxin-producing Escherichia coli O145 strains
                                                        

- (Peer Reviewed Journal)Liao, Y., Salvador, A., Harden, L.A., Liu, F., Lavenburg, V.M., Li, R.W., Wu, V.C. 2019. Characterization of a lytic bacteriophage as an antimicrobial agent for biocontrol of Shiga toxin-producing Escherichia coli O145 strains. Antibiotics. 8(2):74. https://doi.org/10.3390/antibiotics8020074.Characterization of a Lytic Bacteriophage as an Antimicrobial Agent for Biocontrol of Shiga Toxin-Producing Escherichia coli O145 Strains

- (Abstract Only)Quantitative HPLC-MS/MS analysis of toxins in soapberry (Sapindaceae) seeds: Methylenecyclopropylglycine and Hypoglycin A
                                                        

- (Peer Reviewed Journal)Sanford, A.A., Isenberg, S.L., Carter, M., Mojica, M.A., Matthews, T.P., Harden, L.A., Takeoka, G.R., Thomas, J.D., Pirkle, J.L., Johnson, R.C. 2018. Quantitative HPLC-MS/MS analysis of toxins in soapberry (Sapindaceae) seeds: Methylenecyclopropylglycine and Hypoglycin A. Journal of Food Chemistry. 264:449-454. https://doi.org/10.1016/j.foodchem.2018.04.093.Identification of novel bacteriophage peptides using a combination of gene sequence LC-MS-MS analysis and BLASTP

- (Abstract Only)Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves
                                                        

- (Peer Reviewed Journal)Scott, R.A., Thilmony, R.L., Harden, L.A., Zhou, Y., Brandl, M. 2017. Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves. Frontiers in Microbiology. 8:2436. https://doi.org/10.3389/fmicb.2017.02436.Association of acute toxic encephalopathy with litchi consumption in an outbreak in Muzaffarpur, India, 2014: a case-control study
                                                        

- (Peer Reviewed Journal)Shrivastava, A., Kumar, A., Thomas, J., Bhushan, G., Isenberg, S.L., Carter, M., Chhabra, M., Mittal, V., Khare, S., Sejvar, J., Dwivedi, M., Johnson, R., Pirkle, J.L., Yadav, R., Velayudhan, A., Pappanna, M., Singh, P., Somashekar, D., Pradhan, A., Goel, K., Pandey, R., Kumar, M., Kumar, S., Sharer, D., Hall, P., Graham, L., Matthews, T.P., Johnson, D., Schier, J., Chakrabarti, A., Perumal, S., Kumar, R., Valentin, L., Caldwell, K., Jarrett, J., Harden, L.A., Takeoka, G.R., Tong, S., Singh, R., Sing, R., Earhart, K., Dhariwal, A., Chauhan, L., Laserson, K., Venkatesh, S., Srikantiah, P. 2017. Association of acute toxic encephalopathy with litchi consumption in an outbreak in Muzaffarpur, India, 2014: a case-control study. The Lancet Global Health. 5(4):e458-e466. http://dx.doi.org/10.1016/S2214-109X(17)30035-9.Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine

- (Peer Reviewed Journal)Isenberg, S.L., Carter, M.S., Hayes, S.R., Graham, L., Johnson, D., Matthews, T.P., Harden, L.A., Takeoka, G.R., Thomas, J.D., Pirkle, J.L., Johnson, R.C. 2016. Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine. Journal of Agricultural and Food Chemistry. 64(27):5607-5613. doi:10.1021/acs.jafc.6b02478.Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract)

- (Abstract Only)Harden, L.A., Pan, J., Takeoka, G.R. 2015. Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract).  63rd ASMS Conference on Mass Spectrometry and Allied Topic, St. Louis, MO. May 31-June 4, 2015.Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4

- (Peer Reviewed Journal)Zhang, Y., Du, W., Fregevu, C., Kothary, M.H., Harden, L.A., Mchugh, T.H. 2014. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. Journal of Agricultural and Food Chemistry. 62(52):12695-12700. doi: 10.1021/jf50451021.Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties

- (Peer Reviewed Journal)Takeoka, G.R., Dao, L.T., Harden, L.A., Pantoja, A., Kuhl, J.C. 2012. Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties. Journal of Food Chemistry. DOI: 10.1111/j.1365-2621.2012.03174.x.Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California

- (Peer Reviewed Journal)Jay-Russell, M.J., Bates, A.H., Harden, L.A., Miller, W.G., Mandrell, R.E. 2012. Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California. Zoonoses and Public Health. 10.1111/j.1863-2378.2012.01465.x.Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics

- (Peer Reviewed Journal)Fagerquist, C.K., Garbus, B.R., Miller, W.G., Williams, K.E., Yee, E., Bates, A.H., Boyle, S., Harden, L.A., Cooley, M.B., Mandrell, R.E. 2010. Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics. Analytical Chemistry. 82:2717-2725.Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry

- (Peer Reviewed Journal)Fagerquist, C.K., Garbus, B., Williams, K., Bates, A.H., Harden, L.A. 2010. Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry. Journal of American Society for Mass Spectrometry. 21,819-832.Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry

- (Proceedings)Fagerquist, C.K., Garbus, B.R., Williams, K.E., Bates, A.H., Boyle, S., Harden, L.A., Miller, W.G., Mandrell, R.E. 2009. Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry.IN: Proceedings of the 57th American Society of Mass Spectrometry Conference, May 31-June 4, 2009, Philadelphia, PA. DVD:A091307.8125ver.1.pdfWeb-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification

- (Peer Reviewed Journal)Fagerquist, C.K., Garbus, B.R., Williams, K., Bates, A.H., Boyle, S., Harden, L.A. 2009. Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification. Applied and Environmental Microbiology. 75(13):4341-4353.Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry

- (Abstract Only)Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry

- (Peer Reviewed Journal)Lin, J.T., Arcinas, A.J., Harden, L.A. 2009. Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry. Lipids. 44:359-365.SUB-SPECIATING CAMPYLOBACTER JEJUNI BY PROTEOMIC ANALYSIS OF ITS PROTEIN BIOMARKERS AND THEIR POST-TRANSLATIONAL MODIFICATIONS

- (Peer Reviewed Journal)Fagerquist, C.K., Bates, A.H., Heath, S., King, B.C., Garbus, B.R., Harden, L.A., Miller, W.G. Sub-speciating campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications. Journal of Proteome Research. 5:10:2527-2538IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GYLCEROL, AN ACYLGLYCEROL CONTAINING FOUR ACYL CHAINS, IN CASTOR BY LC-ESI-MS

- (Peer Reviewed Journal)Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-gylcerol, an acylglycerol containing four acyl chains, in castor by lc-esi-ms. Journal of Agriculture and Food Chemistry. 54(10):3498-3504.IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GLYCEROL IN CASTOR OIL BY ESI-MS/MS AND ITS BIOSYNTHESIS

- (Abstract Only)Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-glycerol in castor oil by esi-ms/ms and its biosynthesis. American Oil Chemists' Society Meeting.IDENTIFICATION OF BENZALKONIUM CHLORIDE IN COMMERCIAL GRAPEFRUIT SEED EXTRACTS

- (Peer Reviewed Journal)Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Identification of Benzalkonium Chloride in Commercial Grapefruit Seed Extracts. Journal of Agricultural and Food Chemistry. 53:7630-7636.SPECIATION OF CAMPYLOBACTER COLI, C. JEJUNI, C. HELVETICUS, C. LARI, C. SPUTORUM, AND C. UPSALIENSIS BY MATRIX-ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROMETRY

- (Peer Reviewed Journal)Mandrell, R.E., Harden, L.A., Bates, A.H., Miller, W.G., Haddon, W.F., Fagerquist, C.K. 2005. Speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. Applied and Environmental Microbiology. 71:10 p. 6292-6307GENOMIC AND PROTEOMIC IDENTIFICATION OF A DNA-BINDING PROTEIN USED IN THE "FINGERPRINTING" OF CAMPYLOBACTER SPECIES AND STRAINS BY MALDI-TOF-MS PROTEIN BIOMARKER ANALYSIS

- (Peer Reviewed Journal)Fagerquist, C.K., Miller, W.G., Harden, L.A., Bates, A.H., Vensel, W.H., Wang, G., Mandrell, R.E. 2005. Genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. Analytical Chemistry. web release 6/25/05, 77:4897-4907DELPHINIDIN 3-O-(2-O-BETA-D-GLUCOPYRANOSYL-ALPHA-L-ARABINOPYRANOSIDE), A NOVEL ANTHOCYANIN IDENTIFIED IN BELUGA BLACK LENTILS

- (Peer Reviewed Journal)Takeoka, G.R., Dao, L.T., Tamura, H., Harden, L.A. 2005. Delphinidin 3-O-(2-O-Beta-D-Glucopyranosyl-Alpha-L-arabinopyranoside): A Novel Anthocyanin Identified in Beluga Black Lentils. Journal of Agricultural and Food Chemistry. Vol 53. p. 4932-4937.AUTHENTICATION OF COMMERCIAL GRAPEFRUIT SEED EXTRACTS

- (Abstract Only)Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Authentication of commercial grapefruit seed extracts. Meeting Abstract. AFGD Paper No. 50, ACS Nat'l Meeting, March 2005, San Diego, CA.FINGERPRINTING OF CAMPYLOBACTER SPECIES AND STRAINS BY MASS SPECTROMETRIC IDENTIFICATION OF PROTEIN BIOMARKERS

- (Proceedings)Fagerquist, C.K., Harden, L.A., Miller, W.G., Fouts, D., Nelson, K., Mandrell, R.E. 2004. Fingerprinting of campylobacter species and strains by mass spectrometric identification of protein biomarkers. In: Proceedings of the 52nd American Society for Mass Spectrometry Conference and Allied Topics. May 23-27, 2004 Nashville, TN. CDROMBIOCHEMICAL DIVERSITY AMONG THE 1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE ISOZYMES ENCODED BY THE ARABIDOPSIS GENE FAMILY

- (Peer Reviewed Journal)YAMAGAMI, T., TSUCHISAKA, A., YAMADA, K., HADDON, W.F., HARDEN, L.A., THEOLOGIS, A. Biochemical Diversity among the 1-Amino-cyclopropane-1-Carboxylate Synthase Isozymes Encoded by the Arabidopsis Gene Family. JOURNAL OF BIOLOGICAL CHEMISTRY. 2003. V. 278:49102-49112.LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN.

- (Abstract Only)TURNER, C., NGUYEN, T.T., LIN, J.T., WONG, R.Y., HARDEN, L.A., LUNDIN, R.E., MCKEON, T.A. LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN.. BIOCHEMISTRY & MOLECULAR BIOLOGY OF PLANT FATTY ACIDS AND GLYCEROLIPIDS. LIPIDS, v. 38(11)2003, pp:1197-1205


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov