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ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Obesity and Metabolism Research People John Newman





Newman Labheadline bar **NEWMAN LAB** **/ARSUserFiles/37206/Newman Pub Word Cloud 2020.png** /ARSUserFiles/37206/Newman Lab 2020.jpg   Research Scientist
                              **John Newman, Ph.D.**
Associate Adjunct Professor, UC Davis Department of Nutrition
Office: (530) 752-1009
Email address: john.newman2@usda.gov
Google Scholar page
**Research Interests**
The Newman laboratory investigates the impact of dietary lipids on process associated with obesity and inflammation, how these effects alter lipid mediator composition of different biological compartment, and how these cumulative changes influence tissue lipid metabolism and signaling to affect physiology.  This work is focused by an effort to understand whether fine-tuning an individual's dietary lipid intake can improve body weight and health beyond those recommended to the general population by the Dietary Guidelines for Americans.  Moreover, we seek to understand how individual variations in responses to diets and exercise govern the relationship between dietary lipids, lipid mediators and physiological outcomes which influence disease risk and progression.
A major target of our efforts focuses on the measurement of lipids and lipid mediators in experimental models ranging from cell culture, to animal models and human interventions.  Lipids constitute a broad assemblage of important molecules in biochemistry, and we are building and implementing platforms to efficiently measure large number of these chemical species using methods commonly referred to as metabolic profiling or targeted metabolomics. Our current tool box allows us to measure the metabolic cascades described at the right.
The regulation of physiological processes are modulated and mediated by metabolites that are produced in the body, and diet, genetics, and health status can alter the formation of these compounds.  Among these are the lipid mediators a diverse array of metabolites formed from fatty acids with a wide array of physiological impacts.  For instance as shown in the figure below, the metabolism of just one fatty acid, arachidonic acid, can lead to a multitude of products that have major impacts on physiology.
Since the biological responses to dietary lipids often involve the action of bioactive metabolites, too fully understand the mechanisms behind the biological responses to lipids in the diet, we must determine how metabolic products of those lipids change in tissues.  To this end we have begun to explore these questions, and are developing novel analytical chemistry approaches to for this purpose.  For example, below we show that dietary oils change both the lipid and lipid mediator profiles of tissues in different ways, and the mediators within these two tissues are distributed into different lipid sub compartments.
Importantly, such changes would be expected to have fundamental impacts on the normal function of adipose tissue, as the balance of these and other mediators are important regulators of the functional behavior and response of these cell types to external stimuli like inflammation.
Finally, we are very interested in understanding how these bioactive metabolites are trafficked in the body, and are developing analytical approaches to probe the metabolite profiles within sub-compartments in the blood.**Newman Lab Themes**
Improving Public Health by Understanding Diversity in Diet, Body, and Brain Interactions: To refine dietary recommendations and to understand the physiology and pathophysiology of obesity, there is a need to clarify how complex pathways influenced by dietary patterns affect metabolism, behavior, and health outcomes. Importantly, significant person-to-person differences exist with respect to susceptibility to obesity and associated disorders, driven in part by variance in metabolic and behavioral responses to specific environmental factors (i.e., habitual diet, physical activity, stress, and a ‘permissive’ food environment). This work will identify relationships between dietary intake, physical activity, stress, eating behaviors, and metabolic flexibility and encompasses two major WHNRC human phenotyping studies and complementary human, animal model, and in vitro studies.
Individual Metabolism and Physiology Signatures (iMAPS) Study: Understanding how an individual’s metabolism and physiology interact with dietary factors to impact health and health risks is a key to refining how dietary recommendations are developed and delivered to improve the overall health of the American population.  In this study, we recruited overweight women with marginal, yet healthy measures of metabolic syndrome risk factors and randomized them to diets either reflecting the typical American diet as determined by the National Health and Nutrition Examination Survey (https://www.cdc.gov/nchs/nhanes/index.htm).  Participants remained on these diets for eight weeks.  To probe participant metabolic responses to food, and whether those responses changed with time on the diet, individuals were periodically subjected to a mixed nutrient challenge of fat, carbohydrate, and protein, and blood was collected to measure the post-eating (i.e. postprandial) effects on metabolites in their blood.  We are measuring factors in the blood which will report on glucose control, liver health, inflammatory status, blood pressure regulation, as well as factors which modulate hunger.  The primary goal of this research is to determine how diet quality, in the absence of weight loss, effects metabolic risk factors of the metabolic syndrome.
The WHNRC Cross-sectional Phenotyping Study: Inter-individual variability in responses to dietary factors is poorly understood.  To better understand the implications of such variability, we need to first understand the frequency of specific phenotypes within the population, and to determine if these change in association with simple measureable factors including gender, age, and body weight.  To address this knowledge gap, a coordinated research effort at the WHNRC will phenotypically characterize 400 individuals ages 18 – 63 in the normal, overweight and obese body mass categories between Sept of 2015 and Oct of 2019. Study participants will have their metabolism perturbed using a mixed macronutrient challenge and the Newman group will be investigating the postprandial metabolic response in circulating lipids and lipid mediators as a phenotyping tool.
Drug-Vitamin Interactions Mediated by the Thiamine Transporter, SLC19A3: It has been discovered that metformin, a common treatment for hyperglycemia associated with diabetes onset, is a substrate for a major transporter of thiamine (i.e. vitamin B1) in the gut.  Moreover, rare side effects of metformin are reminiscent of those experienced with thiamine deficiency (e.g. encephalopathy). In collaboration with colleagues at the University of California San Francisco, and Tufts’ University, we will investigate the impact of metformin on thiamine absorption using cell culture, animal, and human clinical studies.  Moreover, we will probe the metabolic impacts of thiamine deficiency using metabolomics techniques to fundamentally improve our understanding of the biochemical impact of this key nutrient. The goal of this investigation is to investigate the potential impact of metformin and other thiamine transporter substrates on subclinical thiamine deficiency with relevance to key at risk populations including the elderly.
 Research Team
                              **ARS Employees**
Ira Gray, GS-6 General Physical Scientist
Area of expertise: Sample preparation, LC-MS/MS small molecule analysis
Virginia Artegoitia, GS-11 Research Physiologist and Post-doctoral Scholar
Area of expertise: Animal nutrition, nutritional metabolomics, LC-MS/MS small molecule quantification, multivariate statistical analyses
**UC Davis Employees**
Kamil Borkowski PhD, Assistant Project Scientist III, UC Davis Metabolomics Center
Area of expertise: Adipose physiology, LC-MS/MS small molecule quantification and protein mass spectrometry, multivariate statistical analyses
Ozlem Karaca BS, Staff Research Associate II, UC Davis, Department of Nutrition
Area of expertise: Sample preparation, GC-MS of fatty acids, chemical safety
Anita Wen BS, Graduate Student, UC Davis Pharmacology and Toxicology
Area of expertise: LC-MS/MS small molecule quantification, thiamine metabolism, pharmacokinetics
**Alumni**
Rémy Bosviel, Postdoctoral Researcher, UC Davis Metabolomics Center
Karan Agrawal PhD, Doctoral Student, Pharmacology and Toxicology
William R. Keyes, MS, General Physical Scientist
Michael La Frano, Postdoctoral Scholar, West Coast Metabolomics Center
Brian Piccolo, PhD, Postdoctoral Scholar, Nutritional and Computational Biology
Theresa Pedersen, MS, Analytical Chemist
Aifric O’Sullivan, Postdoctoral Scholar, Nutritional and Computational Biology
Alison (Keenan) Borkowska, PhD, Doctoral Student, Nutritional Biology
Dmitry Grapov, Doctoral Student, Agricultural and Environmental Chemistry
Benjamin Belda, PhD, Postdoctoral Scholar, Nutritional Biology
 Selected Publications(Clicking on the reprint iconReprint Icon  will take you to the publication reprint.)Alpha-linolenic acid-enriched butter attenuates inflammation and insulin resistance by promoting the endogenous synthesis of long-chain n-3 PUFA and anti-inflammatory oxylipins-(Peer Reviewed Journal) Rong, F., Kim, J., You, M., Giraud, D., Shin, S., Kim, S., Borkowski, K., Newman, J.W., Chung, S. 2019. Alpha-linolenic acid-enriched butter attenuates inflammation and insulin resistance by promoting the endogenous synthesis of long-chain n-3 PUFA and anti-inflammatory oxylipins. Journal of Nutritional Biochemistry. 76(108285). https://doi.org/10.1016/j.jnutbio.2019.108285.Comparative analysis of obesity-related cardiometabolic biomarkers in human plasma and serum-(Peer Reviewed Journal) Rajan, M., Sotak, M., Barrenas, F., Shen, T., Borkowski, K., Ashton, N., Biorserud, C., Fandriks, L., Ramstrom, S., Lindahl, T.L., Scholl, M., Lindahl, P., Fiehn, O., Newman, J.W., Perkins, R., Wallenius, V., Lange, S., Borgeson, E. 2019. Comparative analysis of obesity-related cardiometabolic biomarkers in human plasma and serum. Scientific Reports. 15385(2019). https://doi.org/10.1038/s41598-019-51673-0.Luteal metabolomics identifies novel regulators of progesterone production and lipids that may alter functions of luteal immune cells-(Peer Reviewed Journal) Hughes, C.H., Bosviel, R., Newman, J.W., Pate, J.L. 2019. Luteal metabolomics identifies novel regulators of progesterone production and lipids that may alter functions of luteal immune cells. Frontiers in Endocrinology. https://doi.org/10.3389/fendo.2019.00662.Prospective randomized controlled pilot study on the effects of almond consumption on skin lipids and wrinkles-(Peer Reviewed Journal) Foolad, N., Vaughn, A.R., Rybak, I., Burney, W., Chodur, G., Newman, J.W., Steinberg, F.M., Sivamani, R.K. 2019. Prospective randomized controlled pilot study on the effects of almond consumption on skin lipids and wrinkles. Phytotherapy Research. 2019:1-6. https://doi.org/10.1002/ptr.6495.Predicting the effects of supplemental EPA and DHA on the omega-3 index-(Peer Reviewed Journal) Walker, R.E., Harris Jackson, K., Tintle, N.L., Shearer, G.C., Bernasconi, A., Masson, S., Latini, R., Allaire, J., Lamarche, B., Heydari, B., Kwong, R.Y., Flock, M., Kris-Etherton, P., Nedergaard Hedengran, A., Carney, R.M., Skulas-Ray, A., Gidding, S.S., Dewell, A., Trepanowski, J., Gardner, C., Grenon, M.S., Sarter, B., Pedersen, T.L., Newman, J.W., Larson, M., Harris, W.S. 2019. Predicting the effects of supplemental EPA and DHA on the omega-3 index. American Journal of Clinical Nutrition. 110(4):1034-1040. https://doi.org/10.1093/ajcn/nqz161.Oxylipins in triglyceride-rich lipoproteins of dyslipidemic subjects promote endothelial inflammation following a high fat meal-(Peer Reviewed Journal) Rajamani, A., Borkowski, K., Akre, S., Fernandez, A., Newman, J.W., Simon, S.I., Passerini, A.G. 2019. Oxylipins in triglyceride-rich lipoproteins of dyslipidemic subjects promote endothelial inflammation following a high fat meal. Scientific Reports. 9:8655. https://doi.org/10.1038/s41598-019-45005-5.MS-based targeted metabolomics of eicosanoids and other oxylipins: analytical and inter-individual variabilities-(Peer Reviewed Journal) Gladine, C., Ostermann, A.I., Newman, J.W., Schebb, N.H. 2019. MS-based targeted metabolomics of eicosanoids and other oxylipins: analytical and inter-individual variabilities. Journal of Free Radical Biology and Medicine. 144:72-89. https://doi.org/10.1016/j.freeradbiomed.2019.05.012.Walnuts change lipoprotein composition suppressing TNFa-stimulated cytokine production by diabetic adipocyte-(Peer Reviewed Journal) Borkowski, K., Yim, S.J., Holt, R.R., Hackman, R.M., Keen, C.L., Newman, J.W., Shearer, G.C. 2019. Walnuts change lipoprotein composition suppressing TNFa-stimulated cytokine production by diabetic adipocyte. Journal of Nutritional Biochemistry. 68:51-58. https://doi.org/10.1016/j.jnutbio.2019.03.004.Weight loss and fitness intervention increases markers of hepatic bile acid (BA) synthesis, while reducing serum total BA concentrations in sedentary, obese insulin resistant women-(Abstract Only)Impact of post-collection freezing delay on the reliability of serum metabolomics in samples reflecting the California mid-term pregnancy biobank-(Peer Reviewed Journal) La Frano, M.R., Carmichael, S.L., Ma, C., Hardley, M., Shen, T., Wong, R., Rosales, L., Borlowski, K., Pedersen, T.L., Shaw, G., Stevenson, D.K., Fiehn, O., Newman, J.W. 2018. Impact of post-collection freezing delay on the reliability of serum metabolomics in samples reflecting the California mid-term pregnancy biobank. Metabolomics. 14:151. https://doi.org/10.1007/s11306-018-1450-9.Umbilical Cord Blood Metabolomics Reveal Distinct Signatures of Dyslipidemia Prior to Bronchopulmonary Dysplasia and Pulmonary Hypertension-(Peer Reviewed Journal)Diet induced-obesity and weight loss alter bile acid profiles and gene expression in insulin target tissues-(Peer Reviewed Journal) Lafrano, M.R., Hernandez-Carretero, A., Weber, N., Borkowski, K., Pedersen, T.L., Newman, J.W., Osborn, O. 2018.  Diet induced-obesity and weight loss alter bile acid profiles and gene expression in insulin target tissues. Nutrition Research. 46:11-21. https://doi.org/10.1016/j.nutres.2017.07.006.Structural equation modeling of food craving across the menstrual cycle using behavioral, neuroendocrine, and metabolic factors-(Peer Reviewed Journal) Krishnan, S., Tryon, R.R., Agrawal, K., Welch, L.C., Horn, W.F., Newman, J.W., Keim, N.L. 2018. Structural equation modeling of food craving across the menstrual cycle using behavioral, neuroendocrine, and metabolic factors. Physiology and Behavior. 195:28-36. https://doi.org/10.1016/j.physbeh.2018.07.011.Non-invasive profiling of sweat-derived lipid mediators for cutaneous research-() Agrawal, K., Sivamani, R., Newman, J.W. 2018. Non-invasive profiling of sweat-derived lipid mediators for cutaneous research. Skin Research and Technology. 58:188-195. https://doi: 10.1194/jlr.M071738.Oral ibuprofen differentially affects plasma and sweat lipid mediator profiles in healthy adult males-(Peer Reviewed Journal) Agrawal, K., Bosviel, R., Piccolo, B.D., Newman, J.W. 2018. Oral ibuprofen differentially affects plasma and sweat lipid mediator profiles in healthy adult males. Prostaglandins & Other Lipid Mediators. 137:1-8. https://doi.org/10.1016/j.prostaglandins.2018.05.009.Effects of atopic dermatitis and gender on sebum lipid mediator and fatty acid profiles-(Peer Reviewed Journal) Agrawal, K., Hassoun, L.A., Foolad, N., Pedersen, T.L., Sivamani, R.K., Newman, J.W. 2018. Effects of atopic dermatitis and gender on sebum lipid mediator and fatty acid profiles. Prostaglandins Leukotrienes and Essential Fatty Acids. 134:7-16. https://doi:10.1016/j.plefa.2018.05.001.Impact of an 8-week controlled feeding trial based on the Dietary Guidelines for Americans on cardiometabolic health indices-(Peer Reviewed Journal) Krishnan, S., Adams, S.H., Allen, L.H., Laugero, K.D., Newman, J.W., Stephensen, C.B., Burnett, D.J., Witbracht, M.G., Welch, L.C., Que, E.S., Keim, N.L. 2018. Impact of an 8-week controlled feeding trial based on the Dietary Guidelines for Americans on cardiometabolic health indices. American Journal of Clinical Nutrition. 108(2):266-278. https://doi.org/10.1093/ajcn/nqy113.A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction-(Peer Reviewed Journal) Killion, E.A., Reeves, A.R., El Azzouny, M.A., Yan, Q., Surujon, D., Griffin, J.D., Bowman, T.A., Wang, C., Matthan, N.R., Klett, E.L., Kong, D., Newman, J.W., Han, X., Lee, M., Coleman, R.A., Greenberg, A.S. 2018. A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction. Molecular Metabolism. 9:43-46. https://doi.org/10.1016/j.molmet.2018.01.012.A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction-(Peer Reviewed Journal) Killion, E.A., Reeves, A.R., El Azzouny, M.A., Yan, Q., Surujon, D., Griffin, J.D., Bowman, T.A., Wang, C., Matthan, N.R., Klett, E.L., Kong, D., Newman, J.W., Han, X., Lee, M., Coleman, R.A., Greenberg, A.S. 2018. A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction. Molecular Metabolism. 9:43-56. https://doi.org/10.1016/j.molmet.2018.01.012.Abnormal lipoprotein oxylipins in metabolic syndrome and partial correction by omega-3 fatty acids-(Peer Reviewed Journal) Shearer, G.C., Borkowski, K., Puumala, S.L., Harris, W.S., Pedersen, T.L., Newman, J.W. 2017. Abnormal lipoprotein oxylipins in metabolic syndrome and partial correction by omega-3 fatty acids. Prostaglandins Leukotrienes and Essential Fatty Acids. 128:1-10. https://doi.org/10.1016/j.plefa.2017.10.006.Design and implementation of a cross-sectional nutritional phenotyping study in healthy US adults-(Peer Reviewed Journal) Baldiviez, L.M., Keim, N.L., Laugero, K.D., Hwang, D.H., Huang, L., Woodhouse, L.R., Burnett, D.J., Zerofsky, M., Bonnel, E., Allen, L.H., Newman, J.W., Stephensen, C.B. 2017. Design and implementation of a cross-sectional nutritional phenotyping study in healthy US adults. Biomed Central (BMC) Nutrition. 3(79):1-13. https://doi.org/10.1186/s40795-017-0197-4.Establishing and performing targeted multi-residue analysis for lipid mediators and fatty acids in small clinical plasma samples.-() Pedersen, T.L., Newman, J.W. 2018. Establishing and performing targeted multi-residue analysis for lipid mediators and fatty acids in small clinical plasma samples. In: Giera, M, editor. Clinical Metabolomics. Methods in Molecular Biology. Volume 1730. New York, NY: Humana Press. p. 175-212. https://doi.org/10.1007/978-1-4939-7592-1_13Aberrant fatty acid metabolism in the skeletal muscle contributes to insulin resistance in zinc transporter 7 (Znt7) knockout mice-(Peer Reviewed Journal) Huang, L., Tepaamorndech, S., Kirschke, C.P., Newman, J.W., Keyes, W.R., Pedersen, T.L. 2018. Aberrant fatty acid metabolism in the skeletal muscle contributes to insulin resistance in zinc transporter 7 (Znt7) knockout mice. Journal of Biological Chemistry. 293(20):7549-7563. doi:10.1074/jbc.M117.817692.Effects of stimulation technique, anatomical region and time on human sweat lipid mediator profiles.-(Peer Reviewed Journal) Agrawal, K., Waller, J.D., Pedersen, T.L., Newman, J.W. 2017. Effects of stimulation technique, anatomical region and time on human sweat lipid mediator profiles. Prostaglandins & Other Lipid Mediators. 134:84-92. https://doi.org/10.1016/j.prostaglandins.2017.09.007.Obesity-induced changes in lipid mediators persist after weight loss-(Peer Reviewed Journal) Hernandez-Carretero, A., Weber, N., La Frano, M.R., Ying, M., Lantero Rodriguez, J., Sears, D.D., Wallenius, V., Borgeson, E., Newman, J.W., Osborn, O. 2017. Obesity-induced changes in lipid mediators persist after weight loss. International Journal of Obesity. 46:11-21. https://doi.org/10.1016/j.nutres.2017.07.006.Dose-related liver injury of Geniposide associated with the alteration in bile acid synthesis and transportation-(Peer Reviewed Journal) Tian, J., Zhu, J., Yi, Y., Li, C., Zhang, Y., Zhao, Y., Pan, C., Xiang, S., Li, X., Li, J., Newman, J.W., Feng, X., Liu, J., Han, J., Wang, L., La Frano, M.R., Liang, A. 2017. Dose-related liver injury of Geniposide associated with the alteration in bile acid synthesis and transportation. Scientific Reports. 7:8938. https://doi.org/10.1038/s41598-017-09131-2.Associations among fatty acids, desaturase and elongase, and insulin resistance in children-(Peer Reviewed Journal) Beccarelil, L.M., Scheer, R.E., Newman, J.W., Borkowska, A.G., Gray, I.J., Linnell, J.D., Keen, C., Young, H. 2017. Associations among fatty acids, desaturase and elongase, and insulin resistance in children. Journal of American College of Nutrition. 37:44-50. https://doi.org/10.1080/07315724.2017.1347908.The human serum metabolome of vitamin B-12 deficiency and repletion, and associations with neurological function-(Peer Reviewed Journal) Brito, A., Grapov, D., Fahrmann, J., Harvey, D., Green, R., Miller, J.W., Fedosov, S.N., Shahab-Ferdows, S., Hampel, D., Pedersen, T.L., Fiehn, O., Newman, J.W., Uauy, R., Allen, L.H. 2017. The human serum metabolome of vitamin B-12 deficiency and repletion, and associations with neurological function. Journal of Nutrition. 147(10):1839-1849. https://doi.org/10.3945/jn.117.248278.Oleocanthal-rich extra virgin olive oil demonstrates acute anti-platelet effects in healthy men in a randomized trial-(Peer Reviewed Journal) Agrawal, K., Melliou, E., Li, X., Pedersen, T.L., Wang, S.C., Magiatis, P., Newman, J.W., Holt, R.R. 2017. Oleocanthal-rich extra virgin olive oil demonstrates acute anti-platelet effects in healthy men in a randomized trial. Journal of Functional Foods. 36:84-93. doi: 10.1016/j.jff.2017.06.046.Dietary docosahexaenoic acid and trans-10, cis-12-conjugated linoleic acid alter oxylipins profiles in mouse adipose tissue-(Peer Reviewed Journal) Adkins, Y.C., Belda, B.J., Pedersen, T.L., Mackey, B.E., Newman, J.W., Kelley, D.S. 2017. Dietary docosahexaenoic acid and trans-10, cis-12-conjugated linoleic acid alter oxylipins profiles in mouse adipose tissue. Lipids. 52(5):399-413.Prebiotic milk oligosaccharides prevent development of obese phenotype, impairment of gut permeability, and microbial dysbiosis in high fat-fed mice-(Peer Reviewed Journal) Hamilton, K.M., Ronveaux, C.C., Rust, B., Newman, J.W., Hawley, M., Barile, D., Mills, D., Raybould, H.E. 2017. Prebiotic milk oligosaccharides prevent development of obese phenotype, impairment of gut permeability, and microbial dysbiosis in high fat-fed mice. American Journal of Physiology - Gastrointestinal and Liver Physiology. 312:G474–G487. https://doi.org/10.1152/ajpgi.00427.2016.Insulin induces a shift in lipid and primary carbon metabolites in a model of fasting-induced insulin resistance-(Peer Reviewed Journal) Olmstead, K.I., Lafrano, M.R., Fahrmann, J., Grapov, D., Viscarra, J.A., Newman, J.W., Oliver, F., Crocker, D.E., Filipp, F.V., Ortiz, R.M. 2017. Insulin induces a shift in lipid and primary carbon metabolites in a model of fasting-induced insulin resistance. Metabolomics. 13:60. doi: 10.1007/s11306-017-1186-y.Metabolic perturbations of postnatal growth restriction and hyperoxia-induced pulmonary hypertension in a bronchopulmonary dysplasia model-(Peer Reviewed Journal) La Frano, M.R., Fahrmann, J.F., Grapov, D., Fiehn, O., Pedersen, T.L., Newman, J.W., Underwood, M.A., Steinhorn, R.H., Wedgwood, S. 2017. Metabolic perturbations of postnatal growth restriction and hyperoxia-induced pulmonary hypertension in a bronchopulmonary dysplasia model. Metabolomics. 32:13.Acylcarnitines as markers of exercise-associated fuel partitioning, xenometabolism, and potential signals to muscle afferent neurons-(Peer Reviewed Journal) Zhang, J., Light, A.R., Hoppel, C.L., Campbell, C., Chandler, C.J., Burnett, D.J., Souza, E.C., Casazza, G.A., Hughen, R.W., Keim, N.L., Newman, J.W., Hunter, G.R., Fernandez, J.R., Garvey, W.T., Harper, M., Fiehn, O., Adams, S.H. 2017. Acylcarnitines as markers of exercise-associated fuel partitioning, xenometabolism, and potential signals to muscle afferent neurons. Experimental Physiology. 102(1):48-69. doi:10.1113/EP086019.Sweat lipid mediator profiling: a non-invasive approach for cutaneous research-(Abstract Only) Agrawal, K., Hassoun, L., Notay, M., Foolad, N., Sivamani, R.K., Newman, J.W. 2016. Inflammatory Mediator Profiling in Sweat: A Potential Noninvasive Diagnostic for Inflammatory Skin Diseases. Meeting Abstract. LIPID MAPS Annual Meeting 2016, May 17-18, 2016, La Jolla, CA.Circulating levels of endocannabinoids and oxylipins altered by dietary lipids in older women are likely associated with previously identified gene targets-(Peer Reviewed Journal) Watkins, B.A., Kim, J., Kenny, A., Pedersen, T.L., Pappan, K.L., Newman, J.W. 2016. Circulating levels of endocannabinoids and oxylipins altered by dietary lipids in older women are likely associated with previously identified gene targets. BBA Molecular and Cell Biology of Lipids. 1861(11). doi: 10.1016/j.bbalip.2016.07.007.Perinatal triphenyl phosphate exposure accelerates type 2 diabetesonset and increases adipose accumulation in UCD-type 2 diabetesmellitus rats-(Peer Reviewed Journal) Green, A.J., Graham, J.L., Gonzalez, E.A., Lafrano, M.R., He, G., Pertopoulou, S.E., Park, J., Denison, M., Newman, J.W. 2016. Perinatal exposure to the flame retardant triphenyl phosphate accelerates the onset of type 2 diabetes and causes adipose accumulation in UCD-type 2 diabetes mellitus rats. Reproductive Toxicology. pii: S0890-6238(16)30266-0. doi: 10.1016/j.reprotox.2016.07.009.Using a lipidomics approach for nutritional phenotyping in response to a test meal containing gamma-linolenic acid-(Peer Reviewed Journal) Cajka, T., Davis, R., Austin, K.J., Newman, J.W., German, J., Fiehn, O., Smilowitz, J.T. 2016. Using a lipidomics approach for nutritional phenotyping in response to a test meal containing gamma-linolenic acid. Journal of Proteome Research. 12:127. doi: 10.1007/s11306-016-1075-9.Plasma fatty acids, oxylipins, and risk of myocardial infarction: the Singapore Chinese health study-(Peer Reviewed Journal) Sun, Y., Koh, H.W., Choi, H., Koh, W., Yuan, J., Newman, J.W., Su, J., Fang, J., Ong, C., Van Dam, R.M. 2016. Plasma fatty acids, oxylipins, and risk of myocardial infarction: the Singapore Chinese health study. Journal of Lipid Research. 57:1300-1307.Association between plasma endocannabinoids and appetite in hemodialysis patients: a pilot study-(Peer Reviewed Journal) Freidman, A.N., Kim, J., Kaiser, S., Pedersen, T., Newman, J.W., Watkins, B.A. 2016. Association between plasma endocannabinoids and appetite in hemodialysis patients: a pilot study. American Journal of Kidney Diseases. 36(7):658-662. doi: 10.1016/j.nutres.2016.03.009.A randomized placebo controlled trial of ibuprofen for respiratory syncytial infection in a bovine model study-(Peer Reviewed Journal) Walsh, P., Behrens, N., Crvallo Chaigneau, F.R., Mcelligot, H., Agrawal, K., Newman, J.W., Andersen, M., Gershwin, L.J. 2016. A randomized placebo controlled trial of ibuprofen for respiratory syncytial infection in a bovine model study. PLoS One. 11(4):e0152913. doi: 10.1371/journal.pone.0152913.Bile Acid Responses in Methane and Non-Methane Producers to Standard Breakfast Meals-(Abstract Only) Rust, B., La Frano, M., Newman, J.W., Agrawal, K., Horn, W.F., Welch, L., Marco, M., Martin, R., Keim, N.L. 2016. Bile Acid Responses in Methane and Non-Methane Producers to Standard Breakfast Meals. Meeting Abstract. Experimental Biology 2016, San Diego, CA April 2-6, 2016.Intake of farmed Atlantic salmon fed soybean oil increases hepatic levels of arachidonic acid-derived oxylipins and ceramides in mice-(Peer Reviewed Journal) Midtbø, L.K., Borkowska, A.G., Bernhard, A., Rønnevik, A.K., Lock, E., Fitzgerald, M.L., Torstensen, B.E., Liaset, B., Brattelid, T., Pedersen, T.L., Newman, J.W., Kristiansen, K., Madsen, L. 2015. Intake of farmed Atlantic salmon fed soybean oil increases hepatic levels of arachidonic acid-derived oxylipins and ceramides in mice. Journal of Nutritional Biochemistry. 26(6):585-595. doi: 10.1016/j.jnutbio.2014.12.005.The elusive endogenous adipogenic PPARy agonists: lining up the suspects-() Hallenborg, P., Petersen, R.K., Newman, J.W., Madsen, L., Kristiansen, K. 2015. The elusive endogenous adipogenic PPARy agonists: lining up the suspects. Cell Metabolism. 61:149-162. doi: 10.1016/j.plipres.2015.11.002.Effects of Extra Virgin Olive Oil (EVOO) Oleocanthal and Oleacein Content on Platelet Reactivity in Healthy Adults-(Abstract Only) Holt, R.R., Agarwal, K., Li, X., Melliou, E., Pedersen, T., Wang, S., Flynn, D., Magiatis, P., Newman, J.W. 2016. Effects of Extra Virgin Olive Oil (EVOO) Oleocanthal and Oleacein Content on Platelet Reactivity in Healthy Adults. Meeting Abstract. Experimental Biology 2016, San Diego CA Apr. 2-6.Zinc transporter 7 deficiency affects lipid synthesis in adipocytes by inhibiting insulin-dependent Akt activity and glucose uptake-(Peer Reviewed Journal) Tepaamorndech, S., Kirschke, C.P., Pedersen, T.L., Keyes, W.R., Newman, J.W., Huang, L. 2015. Zinc transporter 7 deficiency affects lipid synthesis in adipocytes by inhibiting insulin-dependent Akt activity and glucose uptake. FEBS Journal. 283(2):378-394. doi: 10.1111/febs.13582.Antioxidant supplementation and obesity have independent effects on hepatic oxylipin profiles in insulin resistant, obese-prone rats-(Peer Reviewed Journal) Picklo, M.J., Newman, J.W. 2015. Antioxidant supplementation and obesity have independent effects on hepatic oxylipin profiles in insulin resistant, obese-prone rats. Free Radical Biology and Medicine. 89:182-191.Effects of short-term walnut consumption on human microvascular function and its relationship to plasma epoxide content-() Holt, R.R., Yim, S., Shearer, G.C., Hackman, R.M., Djurica, D., Newman, J.W., Shindel, A.W., Keen, C.L. 2015. Effects of short-term walnut consumption on human microvascular function and its relationship to plasma epoxide content. Journal of Nutritional Biochemistry. 26(12):1458-1466. https://doi.org/10.1016/j.jnutbio.2015.07.012.Dietary DHA reduced downstream endocannabinoid and inflammatory gene expression, epididymal fat mass, and improved aspects of glucose use in muscle in C57BL/6J mice-(Peer Reviewed Journal) Kim, J., Carlson, M.E., Kuchel, G.A., Newman, J.W., Watkins, B.A. 2015. Dietary DHA reduced downstream endocannabinoid and inflammatory gene expression, epididymal fat mass, and improved aspects of glucose use in muscle in C57BL/6J mice. International Journal of Obesity. 40:129–37; doi: 10.1038/ijo.2015.135.Mutations in durum wheat SBEII genes conferring increased amylose and resistant starch affect grain yield components, semolina and pasta quality and fermentation responses in rats-(Peer Reviewed Journal) Hazard, B., Zhang, X., Naemeh, R., Hamilton, K.M., Rust, B., Raybould, H.E., Newman, J.W., Martin, R., Dubcovsky, J. 2015. Mutations in durum wheat SBEII genes conferring increased amylose and resistant starch affect grain yield components, semolina and pasta quality and fermentation responses in rats. Crop Science. 55(6):2813–2825. doi: 10.2135/cropsci2015.03.0179.A novel approach to identify optimal metabotypes of elongase and desaturase activities in prevention of acute coronary syndrome-(Peer Reviewed Journal) Tintle, N.L., Newman, J.W., Shearer, G.C. 2015. A novel approach to identify optimal metabotypes of elongase and desaturase activities in prevention of acute coronary syndrome. Metabolomics. 11(1):1-11. doi: 10.1007/s11306-015-0787-6.Hepatic oxylipin profiles in obese rats: Effect of antioxidant supplementation-(Abstract Only) Picklo, M.J., Newman, J.W. 2015. Hepatic oxylipin profiles in obese rats: Effect of antioxidant supplementation [abstract]. Journal of Federation of American Societies for Experimental Biology. 29:389.2.Short chain fatty acid production and glucose responses by methane producers-(Abstract Only) Rust, B., Gray, I.J., Keifer, D., Newman, J.W., Cooper, D., Welch, L., Horn, W.F., Marco, M., Pelkman, C., Keim, N.L. 2014. Short chain fatty acid production and glucose responses by methane producers. Meeting Abstract. Experimental Biology Meeting, Boston MA, March 28-April 1, 2015.The omega-3 fatty acid DHA dose-dependently reduces atherosclerosis: a putative role for F4-neuroprostanes a specific class of peroxidized metabolites-(Peer Reviewed Journal) Gladine, C., Newman, J.W., Durand, T., Pedersen, T.L., Galano, J., Demougeot, C., Berdeaux, O., Pujos-Guillot, E., Mazur, A., Comte, B. 2014. The omega-3 fatty acid DHA dose-dependently reduces atherosclerosis: a putative role for F4-neuroprostanes a specific class of peroxidized metabolites. PLoS One. 9(11):e111471.Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin-(Peer Reviewed Journal) Campbell, C., Grapov, D., Fiehn, O., Chandler, C.J., Burnett, D.J., Souza, E.C., Casazza, G.A., Gustafson, M.B., Keim, N.L., Newman, J.W., Hunter, G.R., Fernandez, J.R., W. Timothy, G., Harper, M., Hoppel, C.L., Meissen, J.K., Takeuchi, K., Adams, S.H. 2014. Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin. PLoS One. 9(1):e84260. DOI: 10.1371/journal.pone.0084260.Habitual physical activity and plasma metabolomics patterns distinguish individuals with low- versus high-weight loss during controlled energy restriction-(Peer Reviewed Journal) Piccolo, B., Van Loan, M.D., Gertz, E.R., Woodhouse, L.R., Souza, E., Gustafson, M.B., Campbell, C., Fiehn, O., Keim, N.L., Adams, S.H., Newman, J.W. 2015. Habitual physical activity and plasma metabolomics patterns distinguish individuals with low- versus high-weight loss during controlled energy restriction. Journal of Nutrition. 145(4):681-690. doi: 10.3945/jn.114.201574.Effect of omega-3 fatty acids on the oxylipin composition of lipoproteins in hypertriglyceridemic, statin-treated subjects-(Peer Reviewed Journal) Newman, J.W., Pedersen, T.L., Brandenburg, V.R., Harris, W.S., Shearer, G.C. 2014. Effect of omega-3 fatty acids on the oxylipin composition of lipoproteins in hypertriglyceridemic, statin-treated subjects. PLoS One. 9(11):e111471. doi: 10.1371/journal.pone.0111471.Perinatal exposure to the pesticide DDT impairs energy expenditure and metabolism in adult female mice-(Peer Reviewed Journal) La Merrill, M., Karey, E., Moshier, E., Lindtner, C., La Frano, M., Newman, J.W., Buettner, C. 2014. Perinatal exposure to the pesticide DDT impairs energy expenditure and metabolism in adult female mice. PLoS One. 9(7):e103337. doi: 10.1371/journal.pone.0103337.Metabolomics:  Insulin Resistance and Type 2 Diabetes Mellitus-() Adams, S.H., Newman, J.W. 2015. Metabolomics:  Insulin Resistance and Type 2 Diabetes Mellitus. Book Chapter. DOI: 10.1002/9781118387658.ch18.A diet containing a nonfat dry milk matrix significantly alters systemic endocannabinoids and oxylipins in diet-induced obese mice-(Peer Reviewed Journal) Dunn, T.N., Keenan, A.H., Thomas, A.P., Newman, J.W., Adams, S.H. 2014. A diet containing a nonfat dry milk matrix significantly alters systemic endocannabinoids and oxylipins in diet-induced obese mice. Nutrition and Metabolism. 11:1-12.Indomethacin treatment prevents diet-induced obesity and insulin resistance, but not glucose intolerance in C57BL/6J mice-(Peer Reviewed Journal) Fjaere, E., Aune, U., Keenan, A., Ma, T., Lillefosse, H., Xi, Y., Newman, J.W., Haj, F., Liaset, B., Kristiansen, K., Madsen, L. 2014. Indomethacin treatment prevents diet-induced obesity and insulin resistance, but not glucose intolerance in C57BL/6J mice. Journal of Biological Chemistry. DOI: 10.1074/jbc.M113525220.Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice-(Peer Reviewed Journal) Schuster, G.U., Bratt, J., Jiang, X., Pedersen, T.L., Grapov, D., Adkins, Y.C., Kelley, D.S., Newman, J.W., Kenyon, N.J., Stephensen, C.B. 2014. Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice. American Journal of Respiratory Cell and Molecular Biology. doi: 10.1165/rcmb.2013-0136OC.Association between subcutaneous white adipose tissue and serum 25-hydroxyvitamin D in overweight and obese adults-(Peer Reviewed Journal) Piccolo, B.D., Dolnikowski, G., Seyoum, E., Thomas, A., Gertz, E.R., Souza, E.C., Keim, N.L., Adams, S.H., Newman, J.W., Woodhouse, L.R., Van Loan, M.D. 2013. Association between subcutaneous white adipose tissue and serum 25-hydroxyvitamin D in overweight and obese adults. American Journal of Clinical Nutrition. 5(9), 3352-3366. DOI:10.3390/nu5093352.Direct comparison of fatty acid ratios in single cellular lipid droplets as determined by Raman spectroscopy and gas chromatography-(Peer Reviewed Journal) Schie, I.W., Pedersen, T.L., Smith, Z., Wu, J., Newman, J.W., Huser, T., Noltel, L., Yahiatene, I. 2013. Direct comparison of fatty acid ratios in single cellular lipid droplets as determined by Raman spectroscopy and gas chromatography. Analyst. doi: 10.1039/C3AN00970J.High dose simvastatin exhibits enhanced lipid lowering effects relative to simvastatin/ezetimibe combination therapy-(Peer Reviewed Journal) Snowden, S.G., Grapov, D., Nygren, H., Settergren, M., D'Alexandri, F.L., Haeggstrom, J.Z., Hyotylainen, T., Oresic, M., Pedersen, T.L., Newman, J.W., Pernow, J., Wheelock, C.E. 2014. High dose simvastatin exhibits enhanced lipid lowering effects relative to simvastatin/ezetimibe combination therapy. Circulation: Cardiovascular Genetics. 7:955-964. doi: 10.1161/CIRCGENETICS.114.000606.Plasma oxylipin profiling identifies polyunsaturated vicinal diols as responsive to arachidonic acid and docosahexaenoic acid intake in growing piglets-(Peer Reviewed Journal) Bruins, M.J., Dane, A.D., Strassburg, K., Vreeken, R.J., Newman, J.W., Salem, Jr, N., Tyburczy, C., Brenna, J.T. 2013. Plasma oxylipin profiling identifies polyunsaturated vicinal diols as responsive to arachidonic acid and docosahexaenoic acid intake in growing piglets. Journal of Lipid Research. 54(6):1598-1607.Oral or parenteral administration of curcumin does not prevent the growth of high-risk t(4;11) acute lymphoblastic leukemia cells engrafted into a NOD/SCID mouse model-(Peer Reviewed Journal) Zunino, S.J., Storms, D.H., Newman, J.W., Pedersen, T.L., Keen, C.L., Ducore, J.M. 2013. Oral or parenteral administration of curcumin does not prevent the growth of high-risk t(4;11) acute lymphoblastic leukemia cells engrafted into a NOD/SCID mouse model. International Journal of Oncology. 42:741-748. DOI: 10.3892/ijo.2012.1734.The effect of docosahexaenoic acid on t10, c12-conjugated linoleic acid-induced changes in fatty acid composition of mouse liver, adipose and muscle-(Peer Reviewed Journal) Fedor, D.M., Adkins, Y.C., Newman, J.W., Mackey, B.E., Kelley, D.S. 2013. The effect of docosahexaenoic acid on t10, c12-conjugated linoleic acid-induced changes in fatty acid composition of mouse liver, adipose and muscle. Metabolic Syndrome and Disorders. 11(1):63-70. DOI: 10.1089/met.2012.0116.Type 2 diabetes associated changes in the plasma non-esterified fatty acids, oxylipins and endocannabinoids-(Peer Reviewed Journal) Grapov, D., Adams, S.H., Pedersen, T.L., Garvey, W.T., Lok, K.H., Newman, J.W. 2012. Type 2 diabetes associated changes in the plasma non-esterified fatty acids, oxylipins and endocannabinoids. PLoS One. 7(11):e48852.Dietary resveratrol does not delay engraftment, sensitize to vincristine, or inhibit growth of high-risk acute lymphoblastic leukemia cells in NOD/SCID mice-(Peer Reviewed Journal) Zunino, S.J., Storms, D.H., Newman, J.W., Pedersen, T.L., Keen, C.L., Ducore, J.M. 2012. Dietary resveratrol does not delay engraftment, sensitize to vincristine, or inhibit growth of high-risk acute lymphoblastic leukemia cells in NOD/SCID mice. International Journal of Oncology. 41(6):2207-2212. DOI: 10.3892/ijo.2012.1650.Daily consumption of orange-fleshed sweet potato with added fat tends to increase total body vitamin A pool size in vitamin A depleted Bangladeshi women-(Peer Reviewed Journal)IRF-1 and miRNA126 modulate inflammatory VCAM-1 expression in response to a high fat meal-(Peer Reviewed Journal) Sun, C., Alkhoury, K., Wang, Y.I., Foster, G.A., Radecke, C.E., Tam, K., Edwards, C.M., Facciotti, M.T., Armstrong, E.J., Knowlton, A.A., Newman, J.W., Passerini, A.G., Simon, S.I. 2012. IRF-1 and miRNA126 modulate inflammatory VCAM-1 expression in response to a high fat meal. Circulation Research. 111:1054-1064.imDEV: a graphical user interface to R multivariate analysis tools in Microsoft Excel-(Peer Reviewed Journal) Grapov, D., Newman, J.W. 2012. imDEV: a graphical user interface to R multivariate analysis tools in Microsoft Excel. Oxford University Press. doi:10.1093.Vitamin B-12 supplementation of rural Mexican women changes biochemical B-12 status indicators but does not affect hematology or a bone turnover marker-(Peer Reviewed Journal) Shahab-Ferdows, S., Anaya-Loyola, M.A., Vergara-Castaneda, H., Rosado, J., Keyes, W.R., Newman, J.W., Miller, J., Allen, L.H. 5012. Vitamin B-12 supplementation of rural Mexican women changes biochemical B-12 status indicators but does not affect hematology or a bone turnover marker. Journal of Nutrition. vol. 142, no. 10, 1881-1887.Quantitative profiling of oxylipins through comprehensive lc-ms/ms analysis: Application in cardiac surgery-(Peer Reviewed Journal) Strassburg, K., Pedersen, T.L., Huijbrechts, A.M., Kortekaas, K., Lindeman, J., Dane, A., Berger, R., Brenkman, A., Hankemeier, T., Van Duynhoven, J., Kalkhoven, E., Newman, J.W., Vreeken, R. 2012. Quantitative profiling of oxylipins through comprehensive lc-ms/ms analysis: Application in cardiac surgery. Analytical and Bioanalytical Chemistry. 404(5):1413-1426.Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers-(Peer Reviewed Journal) Keenan, A.H., Pedersen, T.L., Fillaus, K., Larson, M.K., Shearer, G.C., Newman, J.W. 2012. Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers. Journal of Lipid Research. doi: 10.1194.Arachidonate 5-lipoxygenase gene variants affect response to fish oil supplementation by healthy African Americans-(Peer Reviewed Journal) Armstrong, P., Kelley, D.S., Newman, J.W., Staggers, F., Hartiala, J., Allayee, H., Stephensen, C.B. 2012. Arachidonate 5-lipoxygenase gene variants affect response to fish oil supplementation by healthy African Americans. Journal of Nutrition. 142(8):1417-1428. DOI: 10.3945/jn.112.159814.Variations in metabolic responses to meal challenges differing in glycemic index in healthy women: Is it meaningful?-(Peer Reviewed Journal) Krishnan, S., Newman, J.W., Hembrooke, T.A., Keim, N.L. 2012. Variations in metabolic responses to meal challenges differing in glycemic index in healthy women: Is it meaningful?. Nutrition and Metabolism. 9:26.Variation in metabolic responses to meal challenges differing in glycemic index in healthy women: Is it meaningful?-(Peer Reviewed Journal) Krishnan, S., Newman, J.W., Hembrooke, T.A., Keim, N.L. 2012. Variation in metabolic responses to meal challenges differing in glycemic index in healthy women: Is it meaningful?. Journal of Nutrition and Metabolism. 9(1):26.Saturated fatty acids activate TLR-mediated pro-inflammatory signaling pathways-(Peer Reviewed Journal) Huang, S., Rutkowsky, J.M., Snodgrass, R.G., Ono-Moore, K., Schneider, D.A., Newman, J.W., Adams, S.H., Hwang, D.H. 2012. Saturated fatty acids activate TLR-mediated pro-inflammatory signaling pathways. Journal of Lipid Research. Epublished. DOI: 10.1194/jlr.D029546.Resveratrol given intraperitoneally does not inhibit growth of high-risk t(4;11) acute lymphoblastic leukemia cells in NOD/SCID mouse model-(Peer Reviewed Journal) Zunino, S.J., Storms, D.H., Newman, J.W., Pedersen, T.L., Keen, C.L., Ducore, J.M. 2012. Resveratrol given intraperitoneally does not inhibit growth of high-risk t(4;11) acute lymphoblastic leukemia cells in NOD/SCID mouse model. International Journal of Oncology. 40(4):1277-84. DOI:10.3892/IJO.2011.1316.An optimized method for measuring methylmalonic acid in low volumes of serum using UPLC-MS/MS-(Peer Reviewed Journal) Pedersen, T.L., Keyes, W.R., Shahab-Ferdows, S., Allen, L.H., Newman, J.W. 2011. An Optimized Method for Measuring Methylmalonic Acid in Low Volumes of Serum Using UPLC-MS/MS. Journal of Chromatography. B. 879, 1502-6.Effects of industrially produced trans fat on markers of systemic inflammation: evidence from a randomized trial in women-(Peer Reviewed Journal) Bendensen, N.T., Stender, S., Szecsi, P.B., Pedersen, S.B., Basu, S., Hellgren, L.I., Newman, J.W., Larsen, T.M., Haugaard, S.B., Astrup, A. 2011. Effects of industrially produced trans fat on markers of systemic inflammation: evidence from a randomized trial in women. Journal of Lipid Research. doi 10.1194/jlr.M014738.ALOX5 gene variants affect eicosanoid production and response to fish oil supplementation-(Peer Reviewed Journal) Stephensen, C.B., Armstrong, P., Newman, J.W., Pedersen, T.L., Legault, J., Schuster, G., Kelley, D.S., Vikman, S., Hartiala, J., Hooman, A. 2011. ALOX5 gene variants affect eicosanoid production and response to fish oil supplementation. Journal of Lipid Research. 52(5):991-1003.The human serum metabolome-(Peer Reviewed Journal) Psychogios, N., Hau, D.D., Peng, J., Guo, A., Mandal, R., Bouatra, S., Sinelnikov, I., Krishnamurthy, R., Eisner, R., Gautam, B., Young, N., Xia, J., Knox, C., Dong, E., Huang, P., Hollander, Z., Pedersen, T.L., Smith, S.R., Bamforth, F., Greiner, R., Mcmanus, B., Newman, J.W., Goodfriend, T., Wishart, D.S. 2011. THE HUMAN SERUM METABOLOME. PLoS One. 6(2): e16957.12- and 15-lipoxygenases in human carotid atherosclerotic lesions: Associations with cerebrovascular symptoms-(Peer Reviewed Journal) Gertow, K., Nobili, E., Folkersen, L., Newman, J.W., Pedersen, T.L., Swedenborg, J., Paulsson-Berne, G., Kuhn, H., Hedin, U., Wheelock, C.E., Hansson, G.K., Haeggstrom, J.Z., Gabrielsen, A. 2011. 12- and 15-lipoxygenases in human carotid atherosclerotic lesions: Associations with cerebrovascular symptoms. Atherosclerosis. 215:411-416.Effects of dynamic exercise on plasma arachidonic acid epoxides and diols in human volunteers-(Peer Reviewed Journal) Giordano, R.M., Newman, J.W., Pedersen, T.L., Ramos, M.I., Stebbins, C. 2011. Effects of dynamic exercise on plasma arachidonic acid epoxides and diols in human volunteers. American Journal of Clinical Nutrition. doi: 21, 471-479.Plasma metabolomic profiles reflective of glucose homeostasis in non-diabetic and Type 2 diabetic obese African-American women-(Peer Reviewed Journal) Fiehn, O., Garvey, W., Newman, J.W., Lok, K.H., Hoppel, C.L., Adams, S.H. 2010. Plasma Metabolomic Profiles Reflective of Glucose Homeostasis in Non-Diabetic and Type 2 Diabetic Obese African-American Women. PLoS One. Vol.5(12): e15234.Impact of a UCP3 Loss-of-Function Polymorphism on the Plasma Metabolomic Profile in Obese African-American Women-(Peer Reviewed Journal) Piccolo, B.D., Grapov, D., Adams, S.H., Hoppel, C., Garvey, W., Fiehn, O., Harper, M., Newman, J.W. 2010. Impact of a UCP3 Loss-of-Function Polymorphism on the Plasma Metabolomic Profile in Obese African-American Women. Journal of Federation of American Societies for Experimental Biology. https://doi.org/10.1371/journal.pone.0015234.s002.Web-enabled and improved software tools and data are needed to measure nutrient intakes and physical activity for personalized health research-(Peer Reviewed Journal) Stumbo, P.J., Weiss, R., Newman, J.W., Pennington, J.A., Tucker, K.L., Wiesenfield, P., Illner, A., Klurfeld, D.M., Kaput, J. 2010. Web-enabled and improved software tools and data are needed to measure nutrient intakes and physical activity for personalized health research. Journal of Nutrition. 140:2104-2115.Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study-(Peer Reviewed Journal) Van Erk, M.J., Wopereis, S., Rubingh, C., Van Vliet, T., Verheij, E., Cnubben, N., Pedersen, T.L., Newman, J.W., Age, S., Van Der Greef, J., Hendriks, H., Van Ommen, B. 2010. Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study. Biomed Central (BMC) Genomics.  3:5.Trans fatty acids adversely affect blood lipids but not intra-abdominal and liver fat deposition - a randomized trial in overweight postmenopausal women-(Peer Reviewed Journal) Bendsen, N.T., Chabanova, E., Thomsen, H.S., Larsen, T.M., Newman, J.W., Stender, S., Dyerberg, J., Haugaard, S.B., Astrup, A. 2010. Trans fatty acids adversely affect blood lipids but not intra-abdominal and liver fat deposition - a randomized trial in overweight postmenopausal women. Nutrition and Diabetes. 1:e4; 10.1038.Detection of omega-3 oxylipins in human plasma and response to treatment with omega-3 acid ethyl esters-(Peer Reviewed Journal) Shearer, G.C., Harris, W.S., Pedersen, T.L., Newman, J.W. 2010. DETECTION OF OMEGA-3 OXYLIPINS IN HUMAN PLASMA AND RESPONSE TO TREATMENT WITH OMEGA-3 ACID ETHYL ESTERS. Journal of Lipid Research. 51(8):2074-81.Impact of circulating esterified eicosanoids and other oxylipins on endothelial function-(Peer Reviewed Journal) Shearer, G., Newman, J.W. 2009. Impact of circulating esterified eicosanoids and other oxylipins on endothelial function. Current Atherosclerosis Reports. Vol. 11:403-410.INCREASED EXPRESSION OF RECEPTORS FOR OREXIGENIC FACTORS IN NODOSE GANGLION OF DIET-INDUCED OBESE RATS-(Peer Reviewed Journal) Paulino, G., Barbier De La Serre, C., Knotts, T.A., Oort, P.J., Newman, J.W., Adams, S.H., Raybould, H. 2009. INCREASED EXPRESSION OF RECEPTORS FOR OREXIGENIC FACTORS IN NODOSE GANGLION OF DIET-INDUCED OBESE RATS. American Journal of Physiology - Endocrinology and Metabolism, 296:898-903.ADMINISTRATION OF A SUBSTITUTED ADAMANTLY-UREA INHIBITOR OF THE SOLUBLE EPOXIDE HYDROLASE PROTECTS THE KIDNEY FROM DAMAGE IN HYPERTENSIVE GOTO-KAKIZAKI RATS-(Peer Reviewed Journal) Olearczyk, J.J., Quigley, J.E., Mitchell, B.C., Yamamoto, T., Kim, I., Newman, J.W., Luria, A., Hammock, B.D., Imig, J.D. 2010. ADMINISTRATION OF A SUBSTITUTED ADAMANTLY-UREA INHIBITOR OF THE SOLUBLE EPOXIDE HYDROLASE PROTECTS THE KIDNEY FROM DAMAGE IN HYPERTENSIVE GOTO-KAKIZAKI RATS. Trade Journal Publication. 10.1042/CS20080039.Plasma Acylcarnitine Profiles Suggest Incomplete Fatty Acid ß-Oxidation and Altered Tricarboxylic Cycle Activity in Type 2 Diabetic African-American Women-(Peer Reviewed Journal) Adams, S.H., Hoppel, C.L., Lok, K.H., Zhao, L., Wong, S.W., Minkler, P.E., Hwang, D.H., Newman, J.W., Garvey, W.T. 2009. Plasma Acylcarnitine Profiles Suggest Incomplete Fatty Acid ß-Oxidation and Altered Tricarboxylic Cycle Activity in Type 2 Diabetic African-American Women. Journal of Nutrition, 139:1073-1081.TRIGLYCERIDE-RICH LIPOPROTEIN LIPOLYSIS RELEASES NEUTRAL AND OXIDIZED FREE FATTY ACIDS THAT INDUCE ENDOTHELIAL CELL INFLAMMATION-(Peer Reviewed Journal) Wang, L., Gill, R., Pedersen, T.L., Higgins, L.J., Newman, J.W., Rutledge, J.C. 2008. Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized free fatty acids that induce endothelial cell inflammation. Journal of Lipid Research.  Papers in press published ahead of print, doi:10.1194/jlr.M700505-JLR200LIPOPROTEIN LIPASE RELEASES ESTERIFIED OXYLIPINS FROM VERY LOW-DENSITY LIPOPROTEINS.-(Peer Reviewed Journal) Shearer, G.C., Newman, J.W. 2008. Lipoprotein lipase releases esterified oxylipins from very low-density lipoproteins. Prostaglandins Leukot. Essent. Fatty Acids. 79:215-222.PHOSPHOLIPASE A2 REDUCTION AMELIORATES COGNITIVE DEFICITS IN MOUSE MODEL OF ALZHEIMER’S DISEASE-(Peer Reviewed Journal) Sanchez-Mejia, R.O., Newman, J.W., Toh, S., Yu, G., Zhou, Y., Scearce-Levie, K., Cheng, I.H., Gan, L., Palop, J.J., Bonventre, J.V., Mucke, L. 2008. PHOSPHOLIPASE A2 REDUCTION AMELIORATES COGNITIVE DEFICITS IN MOUSE MODEL OF ALZHEIMER’S DISEASE. Nature Neuroscience. 11(11): 1311–1318.Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize mik.-(Peer Reviewed Journal) Kim, S., Andaya, C.B., Newman, J.W., Goyal, S.S., Tai, T. 2008. ISOLATION AND CHARACTERIZATION OF A LOW PHYTIC ACID RICE MUTANT REVEALS A MUTATION IN THE RICE ORTHOLOGUE OF MAIZE MIK. Journal of Theoretical and Applied Genetics. 117(8):1291-301.INCREASED BLOOD PRESSURE IN MICE LACKING CYTOCHROME P450 2J5-(Peer Reviewed Journal) Athirakul, K., Bradbury, A.J., Graves, J.P., Degraff, L.M., Ma, J., Zhao, Y., Couse, J.F., Quigley, R., Harder, D.R., Zhao, X., Imig, J.D., Pedersen, T.L., Newman, J.W., Hammock, B.D., Conley, A.J., Korach, K.S., Coffman, T.M., Zeldin, D.C. 2008. INCREASED BLOOD PRESSURE IN MICE LACKING CYTOCHROME P450 2J5. Federation of American Societies for Experimental Biology Conference. 12):4096-108.DEVELOPMENT OF METABOLICALLY STABLE INHIBITORS OF MAMMALIAN MICROSOMAL EPOXIDE HYDROLASE-(Peer Reviewed Journal) Morisseau, C., Newman, J.W., Wheelock, C.E., Hill, Iii, T., Morin, D., Buckpitt, A.R., Hammock, B.D. 2008. Development of metabolically stable inhibitors of mammalian microsomal epoxide hydrolase. Chemical Research in Toxicology. 21:951-957.Effects of pyridine exposure upon structural lipid metabolism in Swiss Webster mice.-(Peer Reviewed Journal) Wheelock, C.E., Forshed, J., Goto, S., Hammock, B.D., Newman, J.W. 2008. Effects of pyridine exposure upon structural lipid metabolism in Swiss Webster mice. Chemical Research in Toxicology. 21;583-590, 2008.SALT LOADING INCREASES URINARY EXCRETION OF LINOLEIC ACID DIOLS AND TRIOLS IN HEALTHY HUMAN SUBJECTS-(Peer Reviewed Journal) Driesbach, A.W., Rice, J.C., Japa, S., Newman, J.W., Sigel, A., Gill, R., Hess, A.E., Cemo, A.C., Fonseca, J.P., Hammock, B.D., Lertora, J., Hamm, J.P. Salt loading increases urinary excretion of linoleic acid diols and triols in healthy human subjects. 2008 Hypertension. 51:755-761.PATHOGENESIS OF ARYL HYDROCARBON RECEPTOR-MEDIATED DEVELOPMENT OF LYMPHOMA IS ASSOCIATED WITH INCREASED CYCLOOXYGENSE-2 EXPRESSION-(Peer Reviewed Journal) Vogel, C., Li, W., Sciullo, E., Newman, J.W., Hammock, B., Reader, R.J., Tuscano, J., Matsumura, F. Pathogenesis of aryl hydrocarbon receptor-mediated development of lymphoma is associated with increased cyclooxygense-2 expression. 2007. American Journal of Pathology. Vol.171, No.5;1538-1548.CLOFIBRATE-INDUCED CHANGES IN THE LIVER, HEART, BRAIN AND WHITE ADIPOSE LIPID METABOLOME OF SWISS-WEBSTER MICE.-(Peer Reviewed Journal) Wheelock, C.S., Goto, S., Hammock, B.D., Newman, J.W. Clofibrate-induced changes in the liver, heart, brain and white adipose lipid metabolome of swiss-webster mice. Metabolomics 3(2):137-145, 2007.Compensatory mechanism for homeostatic blood pressure regulation in Ephx2 gene disrupted mice-(Peer Reviewed Journal) Luria, A., Weldon, S.M., Kabcenell, A.K., Ingraham, R.H., Matera, D., Jiang, H., Gill, R., Morisseau, C., Newman, J.W., Hammock, B.D. Compensatory mechanism for homeostatic blood pressure regulation in Ephx2 gene disrupted mice. 2007. Journal of Biological Chemistry. Vol.282,No.5:12891-2898.Peptidyl-urea based inhibitors of soluble epoxide hydrolases-(Peer Reviewed Journal) Norisseau, C., Newman, J.W., Tsai, H., Baecker, P.A., Hammock, B.D. Peptidyl-urea based inhibitors of soluble epoxide hydrolases. 2006. Bioorganic and Medicinal Chemistry Letters. Vol.16: 5439-5444.ROLE OF SOLUBLE EPOXIDE HYDROLASE IN POST-ISCHEMIC RECOVERY OF HEART CONTRACTILE FUNCTION-(Peer Reviewed Journal) Seubert, J.M., Sinal, C.J., Graves, J.P., Degraff, L.M., Bradbury, A.J., Lee, C.R., Goralski, K.B., Carey, M.A., Luria, A., Newman, J.W., Hammock, B.D., Roberts, H., Rockman, H.A., Murphy, E., Zeldin, D.C. 2006. Role of soluble epoxide hydrolase in post-ischemic recovery of heart contractile function. Journal of Clinical Investigation. 2006. 99:442-450.Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study-(Peer Reviewed Journal) Lee, C.R., North, K.E., Bray, M.S., Fornage, M., Seubert, J.M., Newman, J.W., Hammock, B.D., Couper, D.J., Heiss, G., Zeldin, D.C. Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study. Human Molecular Genetics. 15(10): 1640-1649, 2006.FATTY ACID PROFILES OF LIVER, ADIPOSE TISSUE, SPLEEN, AND HEART OF MICE FED DIETS CONTAINING T10, C-12-, AND C9, T11-CONJUGATED LINOLEIC ADIC-(Peer Reviewed Journal) Kelley, D.S., Bartolini, G.L., Newman, J.W., Vemuri, M., Mackey, B.E. 2006. FATTY ACID PROFILES OF LIVER, ADIPOSE TISSUE, SPLEEN, AND HEART OF MICE FED DIETS CONTAINING T10, C-12-, AND C9, T11-CONJUGATED LINOLEIC ADIC. Prostaglandins Leukotrienes and Essential Fatty Acids. 74:331-338.ATTENUATION OF VASCULAR SMOOTH MUSCLE CELL PROLIFERATION BY 1-CYCLOHEXYL-3-DODECYL UREA IS INDEPENDENT OF SOLUBLE EPOXIDE HYDROLASE INHIBITION.-(Peer Reviewed Journal) Davis, B.B., Morisseau, C., Pedersen, T., Newman, J.W., Hammock, B.D., Weiss, R.H. Attenuation of vascular smooth muscle cell proliferation by 1-cyclohexyl-3-dodecyl urea is independent of soluble epoxide hydrolase inhibition.. American Society of Pharmacology and Experimental Therapeutics. 2006: Vol.316, No.2:815-821.AN EPOXIDE HYDROLASE INHIBITOR, 12-(3-ADAMANTAN-1-YL-UREIDO) DODECANOIC ACID (AUDA), REDUCES ISCHEMIC CEREBRAL INFARCT SIZE IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS.-(Peer Reviewed Journal) Dorrance, A.M., Rupp, N., Pollock, D.M., Newman, J.W., Hammock, J.D., Imig, J.D. An epoxide hydrolase inhibitor, 12-(3-adamantan-1-yl-ureido) dodecanoic acid (auda), reduces ischemic cerebral infarct size in stroke-prone spontaneously hypertensive rats. Journal Cardiovasc Pharmacol. 2005; 46(6):842-848.LIPID SULFATES AND SULFONATES ARE ALLOSTERIC COMPETITIVE INHIBITORS OF THE N-TERMINAL PHOSPHATASE ACTIVITY OF THE MAMMALIAN SOLUBLE EPOXIDE HYDROLASE-(Peer Reviewed Journal) Tran, K.L., Aronov, P.A., Tanaka, H., Newman, J.W., Hammock, B.D., Morisseau, C. Lipid sulfates and sulfonates are allosteric competitive inhibitors of the n-terminal phosphatase activity of the mammalian soluble epoxide hydrolase. Journal of Biochemistry. 44:12179-12187, 2005.AN ORALLY ACTIVE EPOXIDE HYDROLASE INHIBITOR LOWERS BLOOD PRESSURE AND PROVIDES RENAL PROTECTION IN SALT-SENSITIVE HYPERTENSION-(Peer Reviewed Journal) Imig, J.D., Zhao, X., Zaharis, C.Z., Olearczyk, J.J., Pollock, D.M., Newman, J.W., Kim, I.H., Watanabe, T., Hammock, B.D. An orally active epoxide hydrolase inhibitor lowers blood pressure and provides renal protection in salt-sensitive hypertension. Hypertension. 2005;46(part 2):975-981.SOLUBLE EPOXIDE HYDROLASE IS A THERAPEUTIC TARGET FOR ACUTE INFLAMMATION-(Peer Reviewed Journal) Schmelzer, K.R., Kubala, L., Newman, J.W., Kim, I., Eiserich, J.P., Hammock, B.D. Soluble epoxide hydrolase is a therapeutic target for acute inflammation. Journal of the National Academy of Sciences. 102,9772-9777, 2005.THE GRADED EFFECTS OF PROTEINURIA ON HDL STRUCTURE IN NEPHROTIC RATS-(Peer Reviewed Journal) Shearer, G.C., Newman, J.W., Hammock, B.D., Kaysen, G.A. The graded effects of proteinuria on HDL structure in nephrotic rats. Journal of the American Society of Nephrology. 16: 1309-1319, 2005.
 Prominent ProjectsImproving Public Health by Understanding Metabolic and Bio-Behavioral Effects of Following Recommendations in the Dietary Guidelines for AmericansIn-House Appropriated (D)Accession Number:436165OXYGENATE = Oxylipins Signature to Monitor the Cardiometabolic Status and its Response to Dietary InterventionNon-Funded Cooperative Agreement (N)Accession Number:432998West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC)Non-Funded Cooperative Agreement (N)Accession Number:434583Drug-Vitamin Interactions Mediated by the Thiamine Transporter, SLC19A3Reimbursable Cooperative Agreement (R)Accession Number:433904Drug-Vitamin Interactions Mediated by the Thiamine Transporter, SLC19A3Non-Assistance Cooperative Agreement (S)Accession Number:436046Almonds Snacks and Satiety: A Randomized Intervention Trial Examining Acute and Chronic EffectsNon-Assistance Cooperative Agreement (S)Accession Number:436414
 Methods, Technique and Software
                              **Methods and Technique**
• Quantitative analytical chemistry
• Mass spectrometry-based targeted metabolic profiling
• Gas chromatography – mass spectrometry (GC-MS)
• Ultra-performance liquid chromatography  - tandem mass spectrometry (UPLC-MS/MS)
• High performance liquid chromatography - Size Exclusion Chromatography (HPLC-SEC) lipoprotein particle separation
• High-throughput sample processing
• Multi-variate statistics for omic-data integration, visualization, and interpretation
**Software**
Interactive Modules for Data Exploration and Visualization (imDEV) is a multivariate analysis tool box developed in R using an R-Excel interface to function as a Microsoft Excel Add-In.  This software was developed in my group by Dr. Dmitry Grapov.  The Package was first described in 2012 (doi: 10.1093/bioinformatics/bts439.). Dr. Grapov continues to support the software and publically available versions for Microsoft Excel 2003, 2007, and 2010 can be obtained here


        
        
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7/29/2020

    

    
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