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ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Molecular Plant Pathology Laboratory People Robert Davis





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- Con Baker
- Wei Wei
- Rosemarie Hammond
- Robert Davis
- John Hartung
- Ing Ming Lee
- Lev Nemchinov
- Anna Smigocki
- Yan Zhao
- *Spiroplasma kunkelii* genome sequencing project
- Genbank Submissions
- Endopeptidase Alignment of Various Phytoplasmas
- Spiroplasma kunkelli  Plasmid
- Abbreviated CV

Spiroplasma kunkelli  Plasmidheadline bar Davis RE, Dally EL, Jomantiene R, Zhao Y, Roe B, Lin S, and Shao J. 2005. Cryptic plasmid pSKU146 from the wall-less plant pathogenSpiroplasma kunkeliiencodes an adhesin and components of a type IV translocation-related conjugation system.**PLASMID** 53:179-190.   ***Please click on arrows to obtain GenBank records.*** Physical and genetic map of plasmid pSK146 from *Spiroplasma kunkelii*strain CR2-3X. Putative protein-coding regions (open reading frames, ORFs) and direction of transcription are represented by open arrows. palSK1, 58 bp palindromic sequence. IRSK, inverted repeat units potentially forming a hairpin structure. Putative *oriT*A and *oriT*B regions are indicated by broken arrows. Cleavage sites of selected restriction endonucleases are indicated. ABSTRACTA cryptic plasmid of the wall-less plant pathogenic mollicute, *Spiroplasma kunkelii* CR2-3X, was cloned and its sequence analyzed. The 14,615 bp plasmid, designated pSKU146, has a nucleotide content of 28 mol% G+C, and contains 18 potential protein coding regions (open reading frames, ORFs), of which six encode proteins that exhibit similarity to virulence-associated proteins involved in cell-to-cell adhesion or conjugal DNA transfer. One ORF encodes a 96 kDa protein, SkARP1, that is highly similar to SARP1 adhesin involved in attachment of *S. citri* to insect vector gut membrane. Five ORFs encode proteins similar to TraE and Mob in walled bacteria, and to ORFs found in the integrative, conjugative element (ICEF) of *Mycoplasma fermentens*, respectively. Presence of domains similar to proteins of the Type IV secretion system in pathogenic bacteria suggests that spiroplasma possesses a related secretion system. Plasmid pSKU146 also contains two identical *oriT*regions each containing a nick sequence characteristic of the IncP conjugative plasmid family, as well as a 58 bp palindromic sequence, palSK1. Features in pSKU146 suggest that the plasmid functions as a mobile genetic element in conjugative transmission of spiroplasma pathogenicity-related genes.


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