# Patent

## Title
Highly active agonistic CD4 binding site anti-HIV antibodies (HAADS) comprising modified CDRH2 regions that improve contact with GP120

## Abstract

Embodiments of the present invention are directed to compositions and methods for anti-HIV (anti-CD4 binding site) potent VRC01-like (PVL) antibodies targeted to gp120 having an amino acid substitution at a residue in the anti-CD4 binding site PVL antibody that is equivalent to Phe43 in CD4, these antibodies having improved potency and breadth.

## Background
CROSS-REFERENCE TO RELATED APPLICATION(S)
The present application claims priority to and the benefit of U.S. Provisional Application Ser. No. 61/511,425 filed on Jul. 25, 2011, and U.S. Provisional Application Ser. No. 61/523,244 filed on Aug. 12, 2011, the entire contents of both of which are incorporated herein by reference.


STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
This invention was made with government support under P01 AI081677-01, RR008862, and RR022220 awarded by the National Institutes of Health. The government has certain rights in the invention.


INCORPORATION BY REFERENCE
The present application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy having been amended on Jun. 11, 2017, is named “69598SEQLIST.txt” and is 76,303 bytes in size.
TECHNICAL FIELD
This application is directed to a gp120 anti-CD4 binding site (anti-CD4bs) antibody composition that has improved potency and breadth against the human immunodeficiency virus, (HIV) which causes acquired immunodeficiency syndrome (AIDS).
TECHNICAL BACKGROUND
Three decades after the emergence of HIV there is still no vaccine, and AIDS remains a threat to global public health. However, some HIV-infected individuals eventually develop broadly neutralizing antibodies (bNAbs), i.e., antibodies that neutralize a large panel of HIV viruses and that can delay viral rebound in HIV patients. Such antibodies are relevant to vaccine development, as evidenced by the prevention of infection observed after passive transfer to macaques. Antibodies obtained by recent methods target several epitopes on the viral spike gp120 protein. These antibodies show broad and potent activity, and are referred to as highly active agonistic anti-CD4 binding site antibodies (HAADs). HAADS mimic binding of the host receptor CD4 protein by exposing the co-receptor binding site on gp120. Despite isolation from different donors, HAADs are derived from two closely-related Ig VH genes that share gp120 contact residues (Sheid et al., 2011, Science, 333:1633-1637 and Zhou et al., Science, 2010, 329: 811-817.)
Structural analysis of gp120 complexed with VRC01 (a highly potent and broad HAAD), and gp120 complexed with each of VRC03 and VRC-PG04, (two new CD4bs antibodies sharing the VRC01 germline VH gene) revealed convergence of gp120 recognition despite low sequence identities (48-57% in VH; 62-65% in VL) (Wu et al, 2011, Science, 333:1593-1602). However, sequence differences between these clonally-unrelated anti-CD4 antibodies make it difficult to determine the structural features that yield neutralization potency and breadth to thereby obtain a potent HIV antibody that is effective across many HIV strains.
SUMMARY
In some embodiments of the present invention, an isolated anti-CD4 binding site (anti-CD4bs) potentVRC01-like (PVL) antibody composition having a heavy chain and a light chain includes a substituted hydrophobic amino acid in the heavy chain at a position equivalent to Phe43 of a CD4 receptor protein. In some embodiments, the position equivalent to Phe43 of the CD4 receptor protein is position 54 of the heavy chain. In some embodiments, the heavy chain of the anti-CD4bs PVL antibody is selected from SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 45, and/or 46. In some embodiments, the light chain of the anti-CD4bs PVL antibody is selected from SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and/or 43.
In some embodiments of the present invention, the anti-CD4bs PVL antibody is VRC01, VRC02, NIH-45-46, 3BNC60, 3BNC117, 3BNC62, 3BNC95, 3BNC176, 12A21, VRC-PG04, VRC-CH30, VRC-CH31, VRC-CH32, VRC-CH33, VRC-CH34, VRC03 heavy chain with VRC01 light chain, gVRC-H5(d74) heavy chain with VRC-PG04 light chain, gVRC-H12(d74) heavy chain with VRC-PG04 light chain, VRC03, VRC01 heavy chain with VRC03 light chain, 3BNC55, 3BNC91, 3BNC104, 3BNC89, 12A21, or VRC-PG04b.
In some embodiments, the substituted hydrophobic amino acid is phenylalanine, tryptophan, or tyrosine.
In some embodiments of the present invention, the anti-CD4bs PVL antibody is NIH45-46, and the heavy chain position equivalent to Phe43 of the CD4 receptor protein is 54 of NIH-45-46.
In some embodiments of the present invention, a nucleic acid molecule encodes for the heavy chain and light chain of an anti-CD4bs PVL antibody having a substituted hydrophobic amino acid in the heavy chain at the position equivalent to Phe43 of the CD4 receptor protein. In some embodiments, a vector includes the nucleic acid molecule encoding the anti-CD4bs PVL antibody. In some embodiments, a cell includes the vector of the nucleic acid molecule encoding the anti-CD4bs PVL antibody.
In some embodiments of the present invention, a pharmaceutical composition includes the anti-CD4 bs PVL antibody composition or a fragment thereof and a pharmaceutically acceptable carrier.
In some embodiments of the present invention, a method of preventing or treating an HIV infection or an HIV-related disease includes identifying a patient having an HIV infection or an HIV-related disease, and administering to a patient a therapeutically effective amount of an anti-CD4bs PVL antibody as described.
In some embodiments of the present invention, a method of increasing the potency and breadth of an isolated anti-CD4 binding site (anti-CD4bs) potentVRC01-like (PVL) antibody composition having a heavy chain and a light chain includes substituting a target amino acid on the heavy chain with a substitute hydrophobic amino acid, where the target amino acid is at a position on the heavy chain equivalent to the Phe43 of a CD4 receptor protein.



BRIEF DESCRIPTION OF THE DRAWINGS
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawings will be provided by the Office upon request and payment of the necessary fee.
These and other features and advantages of the present invention will be better understood by reference to the following detailed description when considered in conjunction with the accompanying drawings.
FIG. 1A is a superimposition of a structural depiction of NIH45-46 Fab alone (in blue) and of NIH45-46-gp120 complex (in magenta), according to embodiments of the present invention.
FIG. 1B is a structural depiction of NIH45-46-120 (93TH053) complex and the binding interface and domains labeled and colored as indicated, with NIH45-46 Fab shown in magenta (heavy chain) and light purple (light chain), and gp120 shown in yellow (inner domain) and grey (outer domain), according to embodiments of the present invention.
FIG. 2 is a table of the data and refinement statistics from the x-ray diffraction data collected from the NIH45-46 Fab crystal structure and the NIH45-46-120 (93TH057) complex as depicted in FIGS. 1A and 1B, according to embodiments of the present invention.
FIG. 3A is a sequence alignment of the heavy chain variable (VH) domains of NIH45-46 (SEQ ID NO: 6) and VRC01 (SEQ ID NO: 2) antibodies, in which the open circles indicate NIH45-46 side chain residues that contact gp120 and closed circles indicate NIH45-46 main-chain, or main-chain and side chain residues that contact gp120, according to embodiments of the present invention.
FIG. 3B is a sequence alignment of the light chain variable (VL) domains of NIH45-46 (SEQ ID NO: 5) and VRC01 (SEQ ID NO: 1) antibodies, in which the open circles indicate NIH45-46 side chain residues that contact gp120 and closed circles indicate NIH45-46 main-chain, or main-chain and side chain residues that contact gp120, according to embodiments of the present invention.
FIG. 4A is a superimposition and comparison of a structural depiction of NIH45-46-gp120 complex (shown in magenta) and a structural depiction of VRC01-gp120 complex (shown in blue), according to embodiments of the present invention.
FIG. 4B is close-up view of the conserved interactions in the gp120 contacts of NIH45-46 and VRCO1, with the CD4 binding loop of gp120 labeled and shown in yellow, according to embodiments of the present invention.
FIG. 5 is a depiction of the interactions of NIH45-46-gp120 complex and VRC01-gp120 complex with NIH45-46 shown in magenta, VRC01 in blue, and domains of gp120 shown as follows: outer domain (yellow), bridging sheet (orange), CD4 binding loop (blue), V5 loop and D Loop (green), and inner domain (grey); with the contact region between CDRH3 insertion residues of NIH45-46 and gp120 shown in the close-up box with insertion residues 99a-99b labeled alphabetically, according to embodiments of the present invention.
FIG. 6A is a structural depiction of the binding interface of a NIH45-46-gp120 complex characterized by the direct hydrogen bond (dotted line) between the main-chain atom of Gly54NIH45-46 (magenta) and Asp368gp120 (gray) and two water molecules (larger spheres in dotted line), according to aspects of the present invention.
FIG. 6B is a structural depiction of the contact interface of a CD4-gp120 complex, characterized by CD4 (yellow) forming two direct hydrogen bonds (dotted lines) with the CD4-binding loop on gp120, according to embodiments of the present invention.
FIG. 6C is a structural depiction of the contact interface of a VRC03-gp120 complex, characterized by a carbonyl oxygen of Trp54VRC03 forming a hydrogen bond with Asp368gp120, according to embodiments of the present invention.
FIG. 7 is a structural depiction of the binding interface of a NIH45-46-gp120 complex, as shown by a hydrogen bond network between the main-chain carbonyl oxygen of Ala281gp120, Tyr99dNIH45-46 in CDRH3, and Lys52NIH45-46 in CDRH2, in which yellow dots represent hydrogen bonds, and as shown in the inset box: a sulfate ion (yellow) substitutes for Ala281gp120 in the unbound NIH45-46, according to embodiments of the present invention.
FIG. 8 is a structural depiction of the binding interface of a NIH45-46-gp120 complex, as shown by the electrostatic interactions between Asp99cNIH45-46 and Lys97gp120 (lower left dotted line) and hydrogen bonds between Asp99cNIH45-46-Tyr97NIH45-46 (upper left dotted line) and Arg99bNIH45-46-Asn99gp120 (lower right dotted line), according to embodiments of the present invention.
FIG. 9A is a structural depiction of NIH45-46-gp120 complex (with NIH45-46 shown in magenta and gp120 shown in grey) superimposed with a structural depiction of CD4-gp120 complex (with CD4 shown in yellow and gp120 shown in orange), with an arrow and label of Phe43 of CD4, according to embodiments of the present invention.
FIG. 9B is a close-up view of the superimposition of FIG. 9A with the CDRH2 loop of NIH45-46 (magenta) and the CDR2-like loop of CD4 (yellow) interacting with gp120 (grey surface), according to embodiments of the present invention.
FIG. 9C is a structural depiction of a CD4-gp120 (ZM135M.PL10a) complex with the contact interface labeled and colored as in FIG. 1B, and the initial site of CD4 attachment is indicated with the oval, according to embodiments of the present invention.
FIG. 9D is a structural depiction of a NIH45-46-gp120 (93TH057) complex with the contact interface labeled and colored as in FIG. 1B, and the corresponding Phe43CD4 cavity as shown in FIG. 9B is indicated by the asterisk, according to embodiments of the present invention.
FIG. 9E is a structural depiction of a VRC01-gp120 (93TH057) complex with the contact interface labeled and colored as in FIG. 1B, according to embodiments of the present invention.
FIG. 10A is a structural depiction of a NIH45-46-gp120 complex superimposed with a VRC01-gp120 complex, in which the Tyr74 shows different interactions with gp120, and the gp120 bridging sheet is depicted with the broad arrows in gp120 and the asterisks indicate a recombinant Gly2 linker, according to embodiments of the present invention.
FIG. 10B is a close-up view of the structural depiction of FIG. 10A showing the hydrogen bond between Tyr74NIH45-46 and the main-chain carbonyl oxygen of Leu122gp120, according to embodiments of the present invention.
FIG. 11A is a stereo view of a structural depiction of a NIH45-46-gp120 complex superimposed with a VRC01-gp120 complex showing that Tyr28VRC01 LC interacts with an N-linked carbohydrate attached to Asn276gp120 and the side chain counterpart residue Ser28NIH45-46 in the NIH45-46 complex faces away from gp120 to hydrogen bond with Arg64NIH45-46 LC (the arrowheads point to Cα atoms of residue 28 in each structure), according to embodiments of the present invention.
FIG. 11B is a superimposition of NIH45-46LC bound to gp120 (magenta) and unbound (green) showing the hydrogen bonds between Ser28 and Arg64, according to embodiments of the present invention.
FIG. 12 is a structural depiction of gp120 and highlighted differences in the gp120 resurfaced stabilized core 3 (RSC3) variant, in which the NIH45-46 contact surfaces are shown and the RSC3 mutations shown, with labeling and coloring as in FIG. 1B, according to embodiments of the present invention.
FIG. 13A shows sensorgrams from surface plasmon resonance (SPR) experiments of binding experiments of the 93TH057 gp120 protein with NIH45-46 and NIH45-46G54W Fabs, as indicated, and a table of the KD values is shown, according to embodiments of the present invention.
FIG. 13B shows sensorgrams from surface plasmon resonance (SPR) experiments of binding experiments of the CAP244.2.00 D3 gp120 protein with NIH45-46 and NIH45-46G54W Fabs, as indicated, and a table of the KD values is shown, according to embodiments of the present invention.
FIG. 13C shows sensorgrams from surface plasmon resonance (SPR) experiments of binding experiments of the Q259.d2.17 gp120 protein with NIH45-46 and NIH45-46G54W Fabs, as indicated, and a table of the KD values is shown, according to embodiments of the present invention.
FIG. 14 shows neutralization curves for NIH45-46G54W and NIH45-46 in strains DU172.17 and TRO.11, as indicated, according to embodiments of the present invention.
FIG. 15A shows a schematic comparing neutralization potencies of NIH45-46, NIH45-46G54W, NIH45-46G54F, and NIH45-46G54Y, with IC50 values for each color-coded as shown, according embodiments of the present invention.
FIG. 15B shows a graphical comparison of neutralization coverage and potency for VRC01 Monogram (Monogram is a panel of 162 viral strains), VRC01 CAVD (CAVD is a panel of 118 viral strains), PGT121 Monogram, PGT128 Monogram, NIH45-46 CAVD, NIH45-46 hard panel (See Tables 7 and 8), and NIH45-46G54W hard panel, according to embodiments of the present invention.
FIG. 15C shows neutralization summary spider graphs comparing IC50 values for VRC01, NIH45-46, and NIH45-46G54W for 65 common viruses, in which each color represents a different HIV clade, the length of the lines and size of circles are inversely proportional to the IC50 value, the distance between the outer and the inner circle and the distance from the inner circle to the center of a spider graph each span two natural logs in IC50 concentration, the dots on the outer circle indicate strains with IC50 values less than 0.018 μg/ml whose lines were truncated in the graph, and the size of each dot is inversely proportional to the IC50 value, according to embodiments of the present invention.
FIG. 16 is a schematic illustration of, on the left: PVL antibody interactions (magenta and blue-gray) made with gp120 (black); and on the right: CD4 (magenta) with gp120 (black), with the viewpoint of the diagram shown in the inset box, according to embodiments of the present invention.
FIG. 17 is a graph of a neutralization assay showing the effects of mutations at critical residues in YU2 gp120 on neutralization by PVL antibody NIH45-46G54W in which the IC50 values are the mean of several independent experiments, and the graph shows one experiment, according to embodiments of the present invention.
FIG. 18A shows an alignment of the heavy chains of PVL antibodies, their less potent relatives, and their germ-line precursor (SEQ ID NOs. 2, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 45, 46, and 47-59), according to embodiments of the present invention.
FIG. 18B shows an alignment of the light chains of PVL antibodies, their less potent relatives, and their germ-line precursors (SEQ ID NOs. 1, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, and 60-73), according to embodiments of the present invention.



DETAILED DESCRIPTION
Aspects of the present invention are directed to anti-CD4 binding site (CD4bs) antibodies. Embodiments of the present invention include anti-CD4bs antibodies which are potent VRC01-like (PVL) antibodies as defined herein. In some embodiments of the present invention, an anti-CD4bs PVL antibody has a substituted hydrophobic amino acid residue at a position that is equivalent to phenylalanine at position 43 (Phe43) of the host CD4 receptor protein (CD4). In some embodiments of the present invention, a method for increasing the potency and breadth of a PVL antibody includes identifying a target amino acid at the position on the heavy chain of the PVL antibody that is equivalent to Phe43 on CD4, and substituting the target amino acid with a hydrophobic amino acid. For example, in the PVL antibody, NIH45-46, glycine at position 54 (Gly54) is in the Phe43-equivalent position, and substitution of Gly54 in NIH45-46 (Gly54NIH45-46) with a hydrophobic amino acid such as tryptophan, results in NIH45-46G54W which has increased potency and breadth compared to NIH45-46.
Abbreviations for amino acids are used throughout this disclosure and follow the standard nomenclature known in the art. For example, as would be understood by those of ordinary skill in the art, Alanine is Ala or A; Arginine is Arg or R; Asparagine is Asn or N; Aspartic Acid is Asp or D; Cysteine is Cys or C; Glutamic acid is Glu or E; Glutamine is Gln or Q; Glycine is Gly or G; Histidine is His or H; Isoleucine is Ile or I; Leucine is Leu or L; Lysine is Lys or K; Methionine is Met or M; Phenylalanine is Phe or F; Proline is Pro or P; Serine is Ser or S; Threonine is Thr or T; Tryptophan is Trp or W; Tyrosine is Tyr or Y; and Valine is Val or V.
Hydrophobic amino acids are well known in the art. Hydrophobic amino acids include alanine, isoleucine, leucine, methionine, phenylalanine, tryptophan, tyrosine, and valine. In some embodiments of the present invention, an anti-CD4bs PVL antibody has a hydrophobic amino acid substituted at a position equivalent to Phe43 of the CD4 receptor protein, wherein the hydrophobic amino acid is alanine, isoleucine, leucine, methionine, phenylalanine, tryptophan, tyrosine, or valine. In other embodiments, an anti-CD4bs PVL antibody has a hydrophobic amino acid substituted at the position equivalent to Phe43 of CD4 receptor protein, wherein the hydrophobic amino acid is tryptophan, phenylalanine, or tyrosine.
Throughout this disclosure and in embodiments of the present invention, the term “antibody” (Ab) as used herein includes monoclonal antibodies, polyclonal antibodies, multispecific antibodies (for example, bispecific antibodies and polyreactive antibodies), and antibody fragments. Thus, the term “antibody” and “isolated antibody” are used interchangeably herein to refer to an isolated antibody according to embodiments of the present invention. An antibody in any context within this specification is meant to include, but is not be limited to, any specific binding member, immunoglobulin class and/or isotype (e.g., IgG1, IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE and IgM); and biologically relevant fragment or specific binding member thereof, including but not limited to Fab, F(ab′)2, Fv, and scFv (single chain or related entity). It is understood in the art that an antibody is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds, or an antigen binding portion thereof. A heavy chain is comprised of a heavy chain variable region (VH) and a heavy chain constant region (CH1, CH2 and CH3). A light chain is comprised of a light chain variable region (VL) and a light chain constant region (CL). The variable regions of both the heavy and light chains comprise framework regions (FWR) and complementarity determining regions (CDR). The four FWR regions are relatively conserved while CDR regions (CDR1, CDR2 and CDR3) represent hypervariable regions and are arranged from the NH2 terminus to the COOH terminus as follows: FWR1, CDR1, FWR2, CDR2, FWR3, CDR3, FWR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen while, depending on the isotype, the constant region(s) may mediate the binding of the immunoglobulin to host tissues or factors. CDR1, CDR2, and CDR3 of the light chain are referred to as CDRL1, CDRL2 and CDRL3, respectively. CDR1, CDR2, CDR3 of the heavy chain are referred to as CDRH1, CDRH2, and CDRH3, respectively.
Also included in the definition of “antibody” as used herein are chimeric antibodies, humanized antibodies, and recombinant antibodies, human antibodies generated from a transgenic non-human animal, as well as antibodies selected from libraries using enrichment technologies available to the artisan. The term “variable” refers to the fact that certain segments of the variable (V) domains differ extensively in sequence among antibodies. The V domain mediates antigen binding and defines specificity of a particular antibody for its particular antigen. However, the variability is not evenly distributed across the 110-amino acid span of the variable regions. Instead, the V regions consist of relatively invariant stretches called framework regions (FRs) of 15-30 amino acids separated by shorter regions of extreme variability called “hypervariable regions” that are each 9-12 amino acids long. The variable regions of native heavy and light chains each comprise four FRs, largely adopting a beta sheet configuration, connected by three hypervariable regions, which form loops connecting, and in some cases forming part of, the beta sheet structure. The hypervariable regions in each chain are held together in close proximity by the FRs and, with the hypervariable regions from the other chain, contribute to the formation of the antigen-binding site of antibodies. The term “hypervariable region” as used herein refers to the amino acid residues of an antibody that are responsible for antigen binding. The hypervariable region generally comprises amino acid residues from a “complementarity determining region” (“CDR”).
An antibody of the present invention may be a “humanized antibody”. A humanized antibody is considered to be a human antibody that has one or more amino acid residues introduced into it from a source that is non-human. These non-human amino acid residues often are referred to as “import” residues, which typically are taken from an “import” variable region. Humanization may be performed following known methods by substituting import hypervariable region sequences for the corresponding sequences of a human antibody. (See, for example, Jones et al., Nature, 321:522-525 20 (1986); Reichmann et al., Nature, 332:323-327 (1988); Verhoeyen et al., Science, 239:1534-1536 (1988)) the entire contents of each are incorporate herein by reference). Accordingly, such “humanized” antibodies are chimeric antibodies in which substantially less than an intact human variable region has been substituted by the corresponding sequence from a non-human species.
An antibody of the present invention includes an “antibody fragment” which includes a portion of an intact antibody, such as the antigen binding or variable region of the intact antibody. Examples of antibody fragments include, but are not limited to, Fab, Fab′, F(ab′)2, and Fv fragments; diabodies; linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments. (See, for example, U.S. Pat. No. 5,641,870, the entire content of which is incorporated herein by reference.)
Throughout this disclosure and in embodiments of the present invention, a “potent VRC01-like” (“PVL”) antibody of the present invention is an anti-CD4 binding site antibody that has the following conserved heavy chain (HC) and light chain (LC) residues: Arg71HC, Trp50HC, Asn58HC, Trp100BHC, Glu96LC, Trp67LC/Phe67LC, as well as exactly 5 amino acids in CDRL3 domain (using Kabat numbering). (The Kabat numbering system is described in Abhinandan, K. R. and Martin, A. C. R. (2008), “Analysis and improvements to Kabat and structurally correct numbering of antibody variable domains,” Molecular Immunology, 45: 3832-3839, the entire contents of which are herein incorporated by reference.) A PVL antibody of the present invention is any antibody as defined herein, that has the listed PVL features irrespective of the synthesis or derivation of the antibody, irrespective of the other unrestricted domains of the antibody, and irrespective of whether or not other domains of the antibody are present, so long as the antibody has the signature residues and features.
Throughout the disclosure and in embodiments of the present invention, the terms “Phe43-equivalent position” and “Phe43CD4 equivalent position” are used interchangeably and refer to an amino acid position within the heavy chain of a PVL antibody that replicates or mimics the binding pocket and interface contributed by Phe43 of the host CD4 receptor when the CD4 receptor protein is complexed with the HIV viral spike protein gp120. As known in the art, assigned amino acid positions of an antibody do not necessarily correspond to the amino acid residue as numbered from the amino-terminus. Following the Kabat antibody residue/position numbering system, the amino acid residue number may be the same as the amino acid position, but is not necessarily so. (See, Abhinandan, K. R. and Martin, A. C. R. (2008) Molecular immunology, 45: 3832-3839). The structure of the antibody peptide determines the position number. The information for determining position number using the Kabat system for each amino acid in a given sequence can be determined using the information found in Abhinandan and Martin, 2008. Using this position numbering system, the Phe43-equivalent position in a PVL antibody heavy chain sequence can be determined, and substituted with a hydrophobic amino acid to create a similar binding pocket as conferred by Phe43 in CD4. Methods for this mutagenesis are well known in the art (e.g. Example 2).
Subsequent heavy chain sequences can be analyzed using the Kabat numbering system to determine the equivalent position to this position 54. Alternatively, the Phe43CD4-equivalent position can also be determined by structural analysis such as x-ray crystallography. Any means of determining the Phe43CD4-equivalent position may be used so long as the Kabat system is followed as applicable.
For example, the Phe43-equivalent position in NIH45-46 is position 54 as determined by x-ray crystallography and shown herein. The native NIH45-46 sequence contains a glycine at position 54 (Gly54). As such, a PVL antibody substituted with a hydrophobic amino acid at this Phe-43 equivalent position mimics the desired contact interface between the CD4 receptor protein and the CD4 binding site of gp120 (see, e.g., Example 2).
In some embodiments of the present invention, position 54 (Kabat numbering) of the heavy chain of a PVL antibody has a substituted hydrophobic amino acid. Position 54 is determined by analyzing a heavy chain amino acid sequence of a PVL antibody using the Kabat numbering system.
In some embodiments of the present invention, a hydrophobic amino acid is substituted for the “native” amino acid present at the Phe43CD4-equivalent position on the heavy chain of a PVL antibody, where a PVL antibody is an antibody as defined herein having the PVL signature features as described herein, and “native” refers to the amino acid that is present in the PVL antibody prior to substitution. The native amino acid may also be hydrophobic, and may be substituted with another hydrophobic amino acid.
In some embodiments of the present invention, non-limiting examples of PVL antibodies include VRC01, VRC02, NIH-45-46, 3BNC60, 3BNC117, 3BNC62, 3BNC95, 3BNC176, 12A21, VRC-PG04, VRC-CH30, VRC-CH31, VRC-CH32, VRC-CH33, VRC-CH34, VRC03 heavy chain (HC) with VRC01 light chain (LC), gVRC-H5(d74)/VRC-PG04LC, and gVRC-H12(d74)/VRC-PG04LC, VRC03, VRC01 heavy chain (HC) with VRC03 light chain (LC), 3BNC55, 3BNC91, 3BNC104, 3BNC89, 12A21, and VRC-PG04b as listed below in Table 1.







TABLE 1







Examples of PVL Antibodies












Light Chain
Heavy Chain



Antibody Name
SEQ ID NO:
SEQ ID NO:















VRC01
1
2



VRC02
3
4



NIH-45-46
5
6



3BNC60
7
8



3BNC117
9
10



3BNC62
11
12



3BNC95
13
14



3BNC176
15
16



12A12
17
18



VRC-PG04
19
20



VRC-CH30
21
22



VRC-CH31
23
24



VRC-CH32
25
26



VRC-CH33
27
28



VRC-CH34
29
30



VRC03
31
32



3BNC55
33
34



3BNC91
35
36



3BNC104
37
38



3BNC89
39
40



12A21
41
42



VRC-PG04b
43
44



VRC03HC-VRC01LC
1
32



VRC01HC/VRC03LC
31
2



gVRC-H5(d74)/
19
45



VRC-PG04LC



gVRC0H12(D74)/
19
46



VRC-PG04LC










In some embodiments of the present invention, a PVL antibody has a heavy chain selected from one of the heavy chains listed above in Table 1 (SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 45, and 46). Any PVL heavy chain may be matched with a PVL light chain so long as the signature PVL residue features are maintained. In some embodiments, any one of the PVL heavy chains of Table 1 is expressed with any one of the PVL light chains of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43. In other embodiments, any PVL antibody heavy chain can be combined with any PVL antibody light chain.
In embodiments of the present invention, the terms “nucleic acid” and “polynucleotide” are used interchangeably herein to refer to single-stranded or double-stranded RNA, DNA, or mixed polymers. Polynucleotides can include genomic sequences, extra-genomic and plasmid sequences, and smaller engineered gene segments that express, or can be adapted to express polypeptides.
An “isolated nucleic acid” is a nucleic acid that is substantially separated from other genome DNA sequences as well as proteins or complexes such as ribosomes and polymerases, which naturally accompany a native sequence.
In some embodiments of the present invention, nucleic acid molecules encode part or all of the light and heavy chains of the described inventive antibodies, and fragments thereof. Due to redundancy of the genetic code, variants of these sequences will exist that encode the same amino acid sequences.
The present invention also includes isolated nucleic acid molecules encoding the polypeptides of the heavy and the light chain of the PVL antibodies listed in Table 1. In some embodiments, an isolated nucleic acid molecule encodes for any of the PVL heavy chain and light chain polypeptides including those of SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 45, and 46, and SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43, respectively, in which the Phe43CD4-equivalent amino acid (i.e., the target amino acid) of the heavy chain is substituted with a hydrophobic amino acid.
Embodiments of the present invention also include vectors and host cells including a nucleic acid encoding a PVL antibody of the present invention, as well as recombinant techniques for the production of polypeptide of the invention. Vectors of the invention include those capable of replication in any type of cell or organism, including, for example, plasmids, phage, cosmids, and mini chromosomes. In some embodiments, vectors comprising a polynucleotide 5 of the described invention are vectors suitable for propagation or replication of the polynucleotide, or vectors suitable for expressing a polypeptide of the described invention. Such vectors are known in the art and commercially available.
In embodiments of the present invention, “vector” includes shuttle and expression vectors. Typically, the plasmid construct will include an origin of replication (for example, the ColE1 origin of replication) and a selectable marker (for example, ampicillin or tetracycline resistance), for replication and selection, respectively, of the plasmids in bacteria. An “expression vector” refers to a vector that contains the necessary control sequences or regulatory elements for expression of the antibodies including antibody fragment of the invention, in bacterial or eukaryotic cells.
In some embodiments of the present invention, in order to express a polypeptide of the invention, the nucleotide sequences encoding the polypeptide, or functional equivalents, may be inserted into an appropriate expression vector, i.e., a vector that contains the necessary elements for the transcription and translation of the inserted coding sequence. Methods well known to those skilled in the art may be used to construct expression vectors containing sequences encoding a polypeptide of interest and appropriate transcriptional and translational control elements. These methods include in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination. Such techniques are described, for example, in Sambrook, J., et al. (2001) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, Plainview, N.Y., the entire contents of which are incorporated herein by reference.
As used herein, the term “cell” can be any cell, including, but not limited to, eukaryotic cells, such as, but not limited to, mammalian cells or human cells.
In some embodiments of the present invention, the antibodies disclosed herein are produced recombinantly using vectors and methods available in the art. (see, e.g. Sambrook et al., 2001, supra). Human antibodies also can be generated by in vitro activated B cells (see, for example, U.S. Pat. Nos. 5,567,610 and 5,229,275). Reagents, cloning vectors, and kits for genetic manipulation are available from commercial vendors such as BioRad, Stratagene, Invitrogen, ClonTech and Sigma-Aldrich Co.
In some embodiments of the present invention, human antibodies are produced in transgenic animals (for example, mice) that are capable of producing a full repertoire of human antibodies in the absence of endogenous immunoglobulin production. For example, it has been described that the homozygous deletion of the antibody heavy-chain joining region (JH) gene in chimeric and germ-line mutant mice results in complete inhibition of endogenous antibody production. Transfer of the human germ-line immunoglobulin gene array into such germline mutant mice results in the production of human antibodies upon antigen challenge. See, for example, Jakobovits et al., Proc. Natl. Acad. Sci. USA, 90:2551 (1993); Jakobovits et al., Nature, 362:255-258 (1993); Bruggemann et al., Year in Immuno., 7:33 (1993); U.S. Pat. Nos. 5,545,806, 5,569,825, 5,591,669; 5,545,807; and WO 97/17852, the entire contents of all of which are incorporated herein by reference. Such animals can be genetically engineered to produce human antibodies comprising a polypeptide of a PVL antibody of the present invention.
In some embodiments of the present invention, a method includes the preparation and administration of an HIV antibody composition (e.g., a PVL antibody having a hydrophobic amino acid substituted at the Phe43CD4-equivalent position of the PVL heavy chain) that is suitable for administration to a human or non-human primate patient having an HIV infection, or at risk of HIV infection, in an amount and according to a schedule sufficient to induce a protective immune response against HIV, or reduction of the HIV virus, in a human.
In some embodiments of the present invention, a vaccine includes at least one antibody as disclosed herein and a pharmaceutically acceptable carrier. In some embodiments of the present invention, the vaccine is a vaccine including at least one PVL antibody as described herein and a pharmaceutically acceptable carrier. The vaccine can include a plurality of the antibodies having the characteristics described herein in any combination and can further include HIV neutralizing antibodies such as a PVL antibody having the Phe43CD4-equivalent residue on the heavy chain substituted with a hydrophobic amino acid.
In some embodiments of the present invention, carriers as used herein include pharmaceutically acceptable carriers, excipients or stabilizers that are nontoxic to the cell or mammal being exposed thereto at the dosages and concentrations employed. Often the physiologically acceptable carrier is an aqueous pH buffered solution. Examples of physiologically acceptable carriers include, but are not limited to, buffers such as phosphate, citrate, and other organic acids; antioxidants including, but not limited to, ascorbic acid; low molecular weight (less than about 10 residues) polypeptide; proteins, such as, but not limited to, serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as, but not limited to: polyvinylpyrrolidone; amino acids such as, but not limited to: glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides, and other carbohydrates including, but not limited to: glucose, mannose, or dextrins; chelating agents such as, but not limited to: EDTA (ethylenediamineteteraacetic acid); sugar alcohols such as, but not limited to: mannitol or sorbitol; salt-forming counterions such as, but not limited to: sodium; and/or nonionic surfactants such as, but not limited to TWEEN® (polysorbate); polyethylene glycol (PEG), and PLURONICS® (poloxamers).
In some embodiments of the present invention, the compositions may include a single antibody or a combination of antibodies, which can be the same or different, in order to prophylactically or therapeutically treat the progression of various subtypes of HIV infection after vaccination. Such combinations can be selected according to the desired immunity. When an antibody is administered to an animal or a human, it can be combined with one or more pharmaceutically acceptable carriers, excipients or adjuvants as are known to one of ordinary skilled in the art. The composition can further include broadly neutralizing antibodies known in the art, including, for example, a PVL antibody having the Phe43CD4-equivalent residue substituted with a hydrophobic amino acid.
In some embodiments of the present invention, an antibody-based pharmaceutical composition includes a therapeutically effective amount of an isolated HIV antibody which provides a prophylactic or therapeutic treatment choice to reduce infection of the HIV virus. The antibody-based pharmaceutical composition of the present invention may be formulated by any number of strategies known in the art (e.g., see McGoff and Scher, 2000, Solution Formulation of Proteins/Peptides: In McNally, E. J., ed. Protein Formulation and Delivery. New York, N.Y.: Marcel Dekker; pp. 139-158; Akers and Defilippis, 2000, Peptides and Proteins as Parenteral Solutions. In: Pharmaceutical Formulation Development of Peptides and Proteins. Philadelphia, Pa.: Taylor and Francis; pp. 145-177; Akers, et 5 al., 2002, Pharm. Biotechnol. 14:47-127, the entire contents of all of which are incorporated herein by reference).
In some embodiments of the present invention, a method for treating a mammal infected with a virus infection, such as, for example, HIV, comprising administering to said mammal a pharmaceutical composition comprising an HIV antibody composition as disclosed herein. According to some embodiments, the method for treating a mammal infected with HIV includes administering to said mammal a pharmaceutical composition that includes an antibody as disclosed herein, or a fragment thereof. The compositions of embodiments of the present invention may include more than one antibody having the characteristics disclosed herein. For example, a plurality or pool of PVL antibodies, each antibody having the Phe43CD4-equivalent residue substituted with a hydrophobic amino acid.
In some embodiments of the present invention, in vivo treatment of human and non-human patients includes administering or providing a pharmaceutical formulation including an HIV antibody according to embodiments of the present invention. When used for in vivo therapy, the antibodies of the invention are administered to the patient in therapeutically effective amounts (i.e., amounts that eliminate or reduce the patient's viral burden). The antibodies are administered to a human patient, in accord with known methods, such as intravenous administration, for example, as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intracerobrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, oral, topical, or inhalation routes. The antibodies can be administered parenterally, when possible, at the target cell site, or intravenously. In some embodiments, a PVL antibody composition as described herein is administered by intravenous or subcutaneous administration.
In some embodiments of the present invention, a therapeutically effective amount of an antibody is administered to a patient. In some embodiments, the amount of antibody administered is in the range of about 0.1 mg/kg to about 50 mg/kg of patient body weight. Depending on the type and severity of the infection, about 0.1 mg/kg to about 50 mg/kg body weight (for example, about 0.1-15 mg/kg/dose) of antibody is an 5 initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion. The progress of this therapy is readily monitored by conventional methods and assays and based on criteria known to the physician or other persons of skill in the art. The above parameters for assessing successful treatment and improvement in the disease are readily measurable by routine procedures familiar to a physician.
In some embodiments of the present invention, passive immunization using a PVL antibody as disclosed herein, is used as an effective and safe strategy for the prevention and treatment of HIV disease. (See, for example, Keller et al., Clin. Microbiol. Rev. 13:602-14 (2000); Casadevall, Nat. Biotechnol. 20:114 (2002); Shibata et al., Nat. Med. 5:204-10 (1999); and Igarashi et al., Nat. Med. 5:211-16 (1999), each of which are incorporated herein by reference).
The following Examples are presented for illustrative purposes only, and do not limit the scope or content of the present application.
EXAMPLES
Reference is made to Diskin et al. 2013, JEM, 210: 1235-1249; Diskin et al., 2011, Science, 334:12989-1293; and West et al., 2012, PNAS, (doi: 10.1073/pnas.1208984109), the entire contents of all of which are incorporated herein by reference. FIGS. 18A and 18B show the heavy chain (SEQ ID NOs. 2, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 45, 46, and 47-59) and light chain (SEQ ID NOs. 1, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, and 60-73) amino acid sequence alignments of several related variant groups of PVL antibodies as presented in FIG. 2 of West et al. with CDRs defined using the Chothia definition of the Abysis database.
Example 1
Structural Comparisons of NIH45-46 and VRC01
To determine structural correlates of high potency and breadth in HAADs, structures of NIH45-46 alone and bound to the Glade A/E 93TH057 gp120 core were solved (FIGS. 1A, 1B and 2). NIH45-46 is a more potent clonal variant of VRC01 that was isolated from the same donor using a YU2 trimer (Sheid et al., 2011, supra), instead of a resurfaced gp120 core (RSC3) as a bait. Comparisons of NIH45-46 Fab in its free versus gp120-bound states demonstrate that gp120 binding does not require major conformational changes (FIG. 1A). However, gp120 binding induced minor conformational in CDRL1, CDRH3, and in heavy chain framework region 3 (FWR3). As predicted by high sequence identity (85% in VH; 96% in VL) (FIGS. 3A and 3B), NIH45-46 resembles VRC01 (FIGS. 4A and 4B). However, relative to VRC01, NIH45-46 includes a four-residue insertion within CDRH3 (FIG. 5) that was acquired by somatic hypermutation. (See, Sheid et al., 2011, Science, 333:1633-1637, the entire contents of which are incorporated herein by reference.)
The crystal structure of the NIH45-46-93TH057 gp120 complex verified that NIH45-46 targets the CD4bs on gp120 (FIGS. 1B and 5). The primary binding surface is the outer domain, including the CD4 binding loop (FIG. 6A), loop D and loop V5, but CDRH3NIH-45-46 reaches toward the gp120 inner domain (FIG. 1B, 2A-C). Important interactions in the VRC01-93TH057 structure are conserved in NIH45-46 (FIG. 4B); e.g., residues C-terminal to CDRH2 of VRC1 and NIH45-46 mimic the interaction of main-chain atoms in the C′′ β-strand of CD4 domain, which hydrogen bond with the CD4-binding loop of gp120 (FIGS. 6A, 6B, and 6C). In both NIH45-46 and VRC01, hydrogen bonds between CDRH2 and gp120 are water-mediated (except for the Gly54NIH45-46/Gly54VRC01 carbonyl oxygen-Asp368gp120 backbone nitrogen H-bond (FIGS. 6A, 6B, and 6C)), and Arg71VRC01/Arg71NIH45-46 preserves the Arg59CD4 interaction with Asp368gp120. However, the Phe43CD4 interaction with a hydrophobic pocket between α-helix 3gp120 (CD4 binding loop) and β-strand 21gp120 (bridging sheet) (FIGS. 9A and 9B) is not mimicked by either antibody. Differences between VRC01 and NIH45-46 include the conformation of heavy chain residue Tyr74, a FWR3 residue that was substituted during somatic hypermutation (Sheid et al., 2011, supra), and a tyrosine to serine substitution in CDRL1 (FIGS. 10A, 10B, 11A, and 11B).
A notable difference between VRC01 and NIH45-46 is the four-residue insertion (residues 99a-99d) in CDRH3. Three inserted residues contribute to binding to gp120 (FIG. 5—inset), consistent with deletion of the insertion resulting in about 10-fold reduced neutralization potencies (Tables 2 and 3, below).







TABLE 2







In vitro neutralization IC50 values (μg/mL)













NIH45-46
NIH45-46
NIH45-46


Virus
Clade
WT
Y99dA
Δ99a-99d














AC10.0.29
B
0.9
4.4
13


TRO.11
B
1.9
>50
>50


SC422661.8
B
0.05
0.08
1.4


QH0692.42
B
0.7
2.1
3.7


ZM214M.PL15.11
C
0.3
1.1
2.2


CAP45.2.00.G3
C
>50
>50
>50


T257-31
CRF02
0.5
2.4
7.0



(A/G)



















TABLE 3 








CDRH3 sequence









NIH45-46 WT
FCTRGKYCTARDYYNWDFEHWGRGAP



 



NIH45-46 Y99dA
FCTRGKYCTARDAYNWDFEHWGRGAP



 



NIH45-46 Δ99a-99d
FCTRGKYCT----YNWDFEHWGRGAP










First, the Tyr99dNIH45-46 sidechain hydrogen bonds with the loop D Ala281gp120 carbonyl oxygen (FIG. 7), a main-chain atom, thus preventing escape through mutation. Indeed, NIH45-46-sensitive strains accommodate different sidechains at position 281gp120 (Table 4, below).







TABLE 4 







Comparison of in vitro neutralization for viral strains


with differences at 281gp120












Residue
IC50*


Strain
gp120 sequence surrounding residue 281
281gp120
μg/mL





Du156.12
QLLLNGSLAEEEIIIKSENLTDNIKTIIVQLNQSIGINCTRPNNNTRKSV
I
0.01


 


ZM197M.PB7
QLLLNGSLAEEEIIIRSENLTDNTKTIIVHLNESVEIECVRPNNNTRKSV
T
0.14


 


ZM214M.PL15
QLLLNGSLAEKEIMIRSENLTNNAKTIIVQLTEAVNITCMRPGNNTRRSV
A
0.05


 


ZM249M.PL1
QLLLNGSLAEKEIIIRSENITDNVKIIIVHLNESVEINCTRPNNNTRKSI
V
0.02


 


ZM53M.PB12
QLLLNGSTAEEDIIIRSENLTNNAKTIIVHLNESIEIECTRPGNNTRKSI
A
0.65


 


ZM109F.PB4
QLLLNGSLAEEEIVIRSENLTDNAKTIIVHLNKSVEIECIRPGNNTRKSI
A
0.22


 


ZM135M.PL10a
QLLLNGSLSEEGIIIRSKNLTDNTKTIIVHLNESVAIVCTRPNNNTRKSI
T
0.36









The importance of Tyr99dNIH45-46 for potency is demonstrated by alanine substitution (NIH45-46 Y99dA), which reduces the neutralization potency of NIH45-46 to values intermediate between wild-type NIH45-46 and the deletion mutant (Table 2). Second, Asp99cNIH45-46 interacts electrostatically with Lys97gp120 at the base of α-helix 1gp120, and third, Arg99bNIH45-46 hydrogen bonds with Asn99gp120 (FIG. 8). The conformation of the insertion is stabilized by two intramolecular hydrogen bonds. In one, the Tyr99dNIH45-46 sidechain hydrogen bonds with the ε-amino group of Lys52NIH45-46 within CDRH2 (FIG. 7), also seen in the unbound structure of NIH45-46 (FIG. 7—inset), thus the Tyr99dNIH45-46 hydroxyl is poised for interacting with Ala281gp120. A second hydrogen bond between Tyr97NIH45-46 and Asp99cNIH45-46 in the 120-bound Fab positions the negatively-charged aspartic acid for interaction with Lys97gp120 (FIG. 8). The region of gp120 with which CDRH3NIH45-46 interacts was not included in the previously-defined vulnerable site of initial CD4 attachment on the gp120 outer domain (FIG. 9C). Thus, gp120 residues that contact CDRH3NIH45-46 residues required for potent neutralization (Table 2), e.g., Lys97gp120, were mutated in RSC3 (FIG. 12), the resurfaced gp120 used for isolating bNAbs and as a candidate HIV immunogen.
The insertion in CDRH3 contributes to a higher total buried surface area between the NIH45-46 heavy chain and gp120 compared with VRC01 (Table 5, below). The extra contacts with gp120 created by the CDRH3 insertion allow the NIH45-46 footprint on gp120 to more closely resemble the CD4 footprint on gp120 than does the VRC01 footprint (FIGS. 9C, 9D, and 9E, and Tables 5A and 5B, below).







TABLE 5A







Buried Surface Area (Å2)
























Interface
CDR2 +



FWR1
CDR1
FWR2
CDR2
FWR3
CDR3
Total Fab
on gp120
FWR356-65*




















NIH45-46 HC
0
35
51
181
551
326
1144
1097
576


VRC01 HC
0
20
98
136
521
117
892
882
545


NIH45-46 LC
35
8
0
0
0
159
203
192
0


VRC01 LC
36
114
0
0
0
165
314
367
0





*Residues that correspond to the CDR2 region as defined in Zhou et al., Science, 2010, 329: 811-817.






















TABLE 5B







Inner










domain &




Outer



bridging
Loop D +
β-15/α-3 +


domain exit
Total
Interface on



sheet
NAG
NAG
V5
β-24
loop
gp120
Fab or CD4
























NIH45-46
328
335
222
292
81
35
1290
1346


VRC01
157
433
208
328
43
57
1225
1206


CD4
400
136
263
155
14
97
973
1059









The observation that NIH45-46 shows more extensive contacts relative to VRC01 with the inner domain and bridging sheet of gp120 (FIGS. 9D and 9E), yet exhibits higher potency and breadth (Sheid et al., 2001, supra), is inconsistent with the suggestion that increased contact area with regions outside of the outer domain of gp120 correlate with decreased neutralization potency and/or breadth (Zhou et al., 2010 supra; and Wu et al, 2011, Science, 333:1593-1602). Indeed, the observed CDRH3 contacts with the inner domain imply that the crystallographically-observed conformation of this region, whether pre-existing or induced, actively played a role in the affinity maturation events that resulted in the four-residue insertion with CDRH3.
Example 2
Hydrophobic Amino Acid Substitution at Position 54 of NIH45-46
Although NIH45-46 increases its contacts with the inner domain/bridging sheet area of gp120, like VRC01, it lacks a critical CD4 contact to a hydrophobic pocket at the boundary between the gp120 bridging sheet and outer domain made by burying Phe43CD4. This residue alone accounts for 23% of the interatomic contacts between CD4 and gp120, serving as a “linchpin” that welds CD4 to gp120 (Kwong et al., 1998, Nature, 393:648-659). On gp120, the Phe43 binding cavity is a binding site of small-molecule CD4 mimics (Madani et al., 2008, Structure, 16:1689-1701), and a desirable target for compounds to disrupt CD4-gp120 interactions (Kwong et al., 1998, supra), yet it remains unfilled in the 93TH057 complexes with VRC01 (Zhou et al., 2010, supra) and NIH45-46. In a superimposition of a CD4-gp120 structure and NIH45-46-gp120 (FIG. 9B), the Cα atom of heavy chain residue Gly54NIH45-46 is only about 1.4 Å from the Phe43CD4 Cα, suggesting that this important interaction might be mimicked by substituting Gly54NIH45-46 with a large hydrophobic residue. Indeed, residue 54 of VRC03 is a tryptophan, and Trp54VRC03 is accommodated within gp120's Phe43 binding cavity to mimic Phe43CD4, while still maintaining its main-chain hydrogen bond with Asp368gp120 (PDB 3SE8) (FIGS. 6A-6C). If increasing contacts with the inner domain/bridging sheet enhances antibody activity, as suggested by analysis of the NIH45-46-gp120 structure, then substituting Gly54NIH45-46 with a large hydrophobic residue should increase the potency and breadth of NIH45-46.
A series of NIH45-46 mutants were constructed to test the possibility that a hydrophobic sidechain at position 54 in NIH45-46 would improve activity. First it was verified that substitutions at residue 54 did not interfere with antigen binding by assessing the ability of one mutant, NIH45-46G54W, to bind core gp120s. Surface plasmon resonance (SPR) binding analyses demonstrated that NIH45-46G54W Fab bound core gp120s with slightly higher affinities than did NIH45-46 Fab, with differences largely due to slower dissociation rates (FIGS. 13A, 13B, and 13C). Next mutant IgGs were evaluated in neutralization assays using a panel of six viruses chosen to include NIH45-46-sensitive and resistant strains (Table 6, below).









TABLE 6









NIH45-46 IC50 (μg/mL)
















Virus
Clade
WT
G54W
G54F
G54Y
G54I
G54M
G54L
G54H



















SC422661.8
B
0.06
0.03
0.02
0.06
0.1
0.06
0.1
0.09


AC10.0.29
B
0.9
0.2
0.3
0.4
8.6
1.5
1.7
0.6


TRO.11
B
1.0
0.09
0.08
0.1
10
0.3
0.3
0.2


Du172.17
C
>50
0.9
16
>50
>50
>50
>50
>50


CAP210.2.00.E8
C
>50
41
>50
>50
>50
>50
>50
>50


CAP45.2.00.G3
C
>50
6.6
>50
45
>50
>50
>50
>50









NIH45-46G54W and NIH45-46G54F showed increased potencies and NIH45-46G54W increased breadth by neutralizing three strains that are resistant to >50 μg/mL of NIH45-46. The apparent increase in breadth is likely due to increased potency as evidenced by the extrapolated IC50 for NIH45-46 against strain DU172.17 (FIG. 14).
An additional 82 viruses were tested including 13 NIH45-46-resistant, 14 weakly-neutralized, and 55 sensitive strains representing all clades, of which 12 are transmitted founder viruses (FIG. 15A, and Tables 7 and 8, below).







TABLE 7







In vitro neutralization IC50 values (μg/mL) in the “hard panel” of viruses









NIH45-46 IC50 (μg/mL)













Virus
Clade
Category
WT
G54W
G54F
G54Y
















6545.v4.c1
AC
R
>50
18.92
>50
>50


6540.v4.c1
AC
R
>50
>50
>50
>50


CAP45.2.00.G3
C
R
>50
32.25
>50
>50


Du422.1
C
R
>50
>50
>50
>50


CAP210.2.00.E8
C
R
>50
>50
>50
>50


3817.v2.c59
CD
R
>50
>50
>50
>50


89-F1_2_25
CD
R
>50
>50
>50
>50


620345.c01
CRF01_AE
R
>50
>50
>50
>50


T250-4
CRF02_AG
R
>50
1.33
>50
>50


T278-50
CRF02_AG
R
>50
>50
>50
>50


211-9
CRF02_AG
R
>50
16.41
>50
>50


3016.v5.c45
D
R
>50
1.47
5.82
17.89


Du172.17
C
R
>50
3.65
>50
>50


3718.v3.c11
A
P
19.61
0.01
0.32
0.30


703357.C02
CRF01_AE
P
19.17
3.43
7.91
5.60


CNE20
BC
P
7.83
0.04
0.53
0.33


CNE21
BC
P
6.01
0.03
0.12
0.07


HIV-16845-2.22
C
P
5.00
0.45
0.59
0.59


C2101.c01
CRF01_AE
P
4.24
0.04
0.11
0.09


ZM247v1(Rev-)
C
P, T/F
2.94
0.32
0.28
0.42


ZM233M.PB6
C
P
2.50
0.02
0.22
0.16


C1080.c03
CRF01_AE
P
2.48
0.20
0.36
0.29


THRO4156.18
B
P
1.91
0.54
0.89
0.66


3103.v3.c10
ACD
P
1.770
0.200
0.370
0.300


231966.c02
D
P
1.640
0.020
0.060
0.060


TRO.11
B
P
1.610
0.040
0.110
0.080


T251-18
CRF02_AG
P
1.350
0.260
0.410
0.350


Ce1176_A3
C
S, T/F
0.930
0.160
0.240
0.210


QH0692.42
B
S
0.720
0.370
0.560
0.520


T255-34
CRF02_AG
S
0.710
<0.001
0.030
0.040


ZM135M.PL10a
C
S
0.590
0.040
0.130
0.090


AC10.0.29
B
S
0.560
0.130
0.240
0.190


T257-31
CRF02_AG
S
0.490
0.130
0.170
0.180


CNE58
BC
S
0.430
0.020
0.040
0.040


Ce0393_C3
C
S, T/F
0.334
0.013
0.040
0.036


R1166.c01
CRF01_AE
S
0.310
0.130
0.400
0.240


CNE30
BC
S
0.309
0.052
0.100
0.099


CNE17
BC
S
0.261
0.036
0.075
0.073


X2131_C1_B5
G
S
0.230
0.050
0.100
0.100


928-28
CRF02_AG
S
0.230
0.060
0.110
0.120


6535.3
B
S
0.230
0.030
0.070
0.080


ZM53M.PB12
C
S
0.175
0.040
0.080
0.060


ZM214M.PL15
C
S
0.170
0.030
0.090
0.060


Ce703010054_2A2
C
S, T/F
0.159
0.027
0.020
0.022


ZM197M.PB7
C
S
0.150
0.040
0.090
0.070


CAAN5342.A2
B
S
0.150
0.070
0.100
0.100


Q23.17
A
S
0.140
0.010
0.030
0.020


PVO.4
B
S
0.120
0.050
0.070
0.060


1054_07_TC4_1499
B
S, T/F
0.113
0.040
0.076
0.064


Ce2010_F5
C
S, T/F
0.101
0.038
0.046
0.049


ZM109F.PB4
C
S
0.095
0.002
0.022
0.026


1056_10_TA11_1826
B
S, T/F
0.094
0.024
0.064
0.044


0330.v4.c3
A
S
0.090
0.030
0.040
0.030


P1981_C5_3
G
S
0.080
0.020
0.030
0.040


Q461.e2
A
S
0.076
0.009
0.030
0.023


P0402_c2_11
G
S
0.073
0.003
0.008
0.012


SC422661.8
B
S
0.060
0.020
0.040
0.040


62357_14_D3_4589
B
S, T/F
0.060
0.020
0.040
0.030


WITO4160.33
B
S
0.060
0.010
0.020
0.030


Ce2060_G9
C
S, T/F
0.058
0.005
0.022
0.021


Ce0682_E4
C
S, T/F
0.054
0.010
0.011
0.017


231965.c01
D
S
0.051
<0.001
0.022
0.025


Q259.d2.17
A
S
0.043
<0.001
0.009
0.009


TRJO4551.58
B
S
0.040
0.010
0.030
0.030


6811.v7.c18
CD
S
0.035
<0.001
0.017
0.011


R2184.c04
CRF01_AE
S
0.034
0.005
0.015
0.015


6480.v4.c25
CD
S
0.032
0.004
0.014
0.018


X1254_c3
G
S
0.032
0.002
0.011
0.013


Q842.d12
A
S
0.031
0.005
0.011
0.015


C3347.c11
CRF01_AE
S
0.029
<0.001
0.015
0.011


1006_11_C3_1601
B
S, T/F
0.027
<0.001
0.003
0.005


3415.v1.c1
A
S
0.022
<0.001
<0.001
<0.001


X1193_c1
G
S
0.021
<0.001
<0.001
0.006


Du156.12
C
S
0.020
<0.001
<0.001
0.007


RHPA4259.7
B
S
0.017
<0.001
0.005
0.007


ZM249M.PL1
C
S
0.017
0.002
0.004
0.003


0815.v3.c3
ACD
S
0.014
<0.001
<0.001
<0.001


REJO4541.67
B
S
0.014
0.002
0.007
0.007


3301.v1.c24
AC
S
0.009
<0.001
0.001
0.003


Q769.d22
A
S
0.009
<0.001
0.005
0.007


CNE53
BC
S
0.008
<0.001
0.005
0.006


WEAU_d15_410_787
B
S, T/F
0.005
<0.001
<0.001
0.002


Geometric means


0.417
0.046
0.120
0.124





Category


R—Resistant


P—Poorly sensitive


S—Sensitive


T/F—Transmitted Founder













TABLE 8







In vitro neutralization IC80 values (μg/mL) in the “hard panel” of viruses









NIH45-46 IC80 (μg/mL)













Virus
Clade
Category
WT
G54W
G54F
G54Y
















T250-4
CRF02_AG
R
>50
44.94
>50
>50


703357.C02
CRF01_AE
R
>50
17.61
>50
30.58


CAP45.2.00.G3
C
R
>50
>50
>50
>50


CNE20
BC
R
>50
0.48
3.91
2.40


CAP210.2.00.E8
C
R
>50
>50
>50
>50


T278-50
CRF02_AG
R
>50
>50
>50
>50


211-9
CRF02_AG
R
>50
>50
>50
>50


620345.c01
CRF01_AE
R
>50
>50
>50
>50


3016.v5.c45
D
R
>50
15.37
36.97
>50


3817.v2.c59
CD
R
>50
>50
>50
>50


89-F1_2_25
CD
R
>50
>50
>50
>50


6540.v4.c1
AC
R
>50
>50
>50
>50


6545.v4.c1
AC
R
>50
>50
>50
>50


Du422.1
C
P
>50
>50
>50
>50


3718.v3.c11
A
P
>50
0.04
4.620
3.85


Du172.17
C
P
>50
42.85
>50
>50


CNE21
BC
P
38.07
0.16
0.66
0.29


C2101.c01
CRF01_AE
P
31.37
0.17
0.42
0.27


ZM247v1(Rev-)
C
P, T/F
24.50
2.60
2.45
3.57


HIV-16845-2.22
C
P
22.61
2.10
2.75
2.75


ZM233M.PB6
C
P
14.18
0.11
0.99
0.78


C1080.c03
CRF01_AE
P
11.56
0.91
2.26
1.83


231966.c02
D
P
9.64
0.11
0.24
0.23


THRO4156.18
B
P
8.22
1.81
3.01
2.14


TRO.11
B
P
7.49
0.13
0.30
0.22


3103.v3.c10
ACD
P
6.15
0.56
1.28
0.81


T251-18
CRF02_AG
P
3.68
0.92
1.38
1.16


T255-34
CRF02_AG
S
3.442
0.099
0.198
0.174


Ce1176_A3
C
S, T/F
3.17
0.45
0.83
0.58


ZM135M.PL10a
C
S
2.79
0.16
0.43
0.30


CNE58
BC
S
2.08
0.05
0.11
0.11


AC10.0.29
B
S
1.93
0.63
1.12
0.90


QH0692.42
B
S
1.71
1.12
1.65
1.50


T257-31
CRF02_AG
S
1.38
0.45
0.51
0.67


R1166.c01
CRF01_AE
S
1.21
0.51
1.32
0.84


CNE30
BC
S
1.067
0.196
0.348
0.263


Ce0393_C3
C
S, T/F
0.936
0.089
0.173
0.134


X2131_C1_B5
G
S
0.88
0.24
0.41
0.39


CNE17
BC
S
0.734
0.127
0.287
0.264


928-28
CRF02_AG
S
0.64
0.25
0.41
0.33


ZM53M.PB12
C
S
0.61
0.16
0.23
0.22


ZM214M.PL15
C
S
0.59
0.15
0.30
0.23


ZM197M.PB7
C
S
0.55
0.18
0.23
0.21


6535.3
B
S
0.54
0.13
0.27
0.24


Ce703010054_2A2
C
S, T/F
0.538
0.077
0.070
0.070


Q23.17
A
S
0.50
0.03
0.07
0.06


1056_10_TA11_1826
B
S, T/F
0.447
0.160
0.283
0.189


ZM109F.PB4
C
S
0.437
0.070
0.17
0.168


PVO.4
B
S
0.41
0.16
0.25
0.18


1054_07_TC4_1499
B
S, T/F
0.404
0.165
0.283
0.236


CAAN5342.A2
B
S
0.40
0.21
0.28
0.27


Ce2010_F5
C
S, T/F
0.357
0.187
0.186
0.235


0330.v4.c3
A
S
0.3
0.11
0.13
0.09


Q461.e2
A
S
0.291
0.091
0.135
0.103


Ce2060_G9
C
S, T/F
0.290
0.042
0.085
0.068


WITO4160.33
B
S
0.26
0.04
0.14
0.09


P1981_C5_3
G
S
0.24
0.07
0.11
0.09


P0402_c2_11
G
S
0.214
0.023
0.047
0.049


1006_11_C3_1601
B
S, T/F
0.196
0.008
0.024
0.021


62357_14_D3_4589
B
S, T/F
0.19
0.07
0.14
0.09


Ce0682_E4
C
S, T/F
0.155
0.039
0.056
0.065


Q259.d2.17
A
S
0.154
0.014
0.036
0.034


SC422661.8
B
S
0.13
0.07
0.10
0.09


TRJO4551.58
B
S
0.13
0.05
0.08
0.07


R2184.c04
CRF01_AE
S
0.127
0.036
0.054
0.045


231965.c01
D
S
0.126
0.035
0.062
0.054


6811.v7.c18
CD
S
0.113
0.033
0.063
0.059


X1254_c3
G
S
0.107
0.018
0.043
0.041


6480.v4.c25
CD
S
0.100
0.021
0.046
0.051


C3347.c11
CRF01_AE
S
0.094
0.028
0.059
0.052


3415.v1.c1
A
S
0.086
0.023
0.029
0.037


Q842.d12
A
S
0.073
0.025
0.039
0.045


X1193_c1
G
S
0.064
0.009
0.026
0.024


Du156.12
C
S
0.054
0.005
0.019
0.026


ZM249M.PL1
C
S
0.053
0.007
0.016
0.011


0815.v3.c3
ACD
S
0.052
0.003
0.014
0.015


RHPA4259.7
B
S
0.047
0.007
0.020
0.020


CNE53
BC
S
0.039
0.005
0.024
0.027


REJO4541.67
B
S
0.035
0.013
0.028
0.020


3301.v1.c24
AC
S
0.033
0.004
0.011
0.014


Q769.d22
A
S
0.033
0.009
0.023
0.024


WEAU_d15_410_787
B
S, T/F
0.015
0.003
0.004
0.008


Geometric means


1.231
0.225
0.437
0.393





Category


R—Resistant


P—Poorly sensitive


S—Sensitive


T/F—Transmitted Founder






The above panel of viruses in Tables 7 and 8 (referred to as the “hard panel”) is more difficult for NIH45-46 to neutralize than a recently-published panel (Sheid et al, 2011, supra) (FIG. 15B). NIH45-46G54W showed increased potency and breadth compared to NIH45-46 and VRC01: geometric mean IC50s of 0.04 μg/mL for NIH45-46G54W, 0.41 μg/mL for NIH45-46, and 0.92 μg/mL for VRC01 (calculated for 65 viruses against which VRC01 was previously evaluated (Sheid et al, 2011, supra) (Tables 7 and 8, and FIG. 15C). (Geometric IC50S values were calculated without excluding resistant strains by entering values of 50 μg/ml for strains with IC50 values greater than 50 μg/ml)







TABLE 9







Sequence correlates of resistance to NIH45-46







Strain













620345_c1
Ser456 (Arg)
Asp459 (Gly)
Lys279 (Asn/Asp)


89_F1_2_25
Ser456 (Arg)
Asn458 (Gly)


6540_v4_c1
Ser456 (Arg)
Tyr458 (Gly)
Ser280 (Asn)


6545_v4_c1
Ser456 (Arg)
Tyr458 (Gly)
Ser280 (Asn)


Du422.1
Trp456 (Arg)


T250_4
Pro459 (Gly)


T278_50
Glu459 (Gly)
Ala279 (Asn/Asp)


Ce1172_H1
deletion of Gly459


X2088_c9
Val459 (Gly)


H086.8
Asp459 (Gly)
Lys279 (Asn/Asp)









As shown in Table 9, above, 10 of 17 NIH45-46-resistant strains (5 of 7 NIH45-46G54W-resistant strains) have amino acid variations at NIH45-46-contacting residues that have a fully conserved residue (shown in parenthesis) in all NIH45-46 sensitive strains. These mutations occur in the β23 strand immediately preceding V5 and in loop D. The positions of underlined sites have been shown to be important in resistance to VRC01 as reported in Li et al., 2011, J. Virol., 85:8954-8967.
The largest difference between sensitivity to NIH45-46 and sensitivity to VRC01 was in strain 3016.v5.c45 (IC50s of>30 and 0.16 μg/mL, respectively). The most notable residue in 3016.v5.c45 is Tyr282 in loop D. This large residue may alter the conformation of loop D, which is closely contacted by the four-residue insertion in the NIH45-46 CDRH3. The absence of the insertion may permit VRC01 to better accommodate an altered loop D. The next largest NIH45-46/VRC01 difference, for strain C2101.c1 (12.78 vs. 0.36 μg/mL), may similarly relate to the unusual Lys99gp120 residue replacing the asparagine that favorably interacts with Arg99bNIH45-46 in the NIH45-46-gp120 crystal structure.
From the neutralization assays, it is noted that NIH45-46G54W gained de novo neutralization activity against six NIH45-46 resistant strains, including the only three that were sensitive to VRC01 but resistant to NIH45-46 in the panel tested in Sheid et al, 2011, supra. For some strains that NIH45-46 neutralizes poorly, NIH45-46G54W was significantly more potent (e.g., improvements of >700-fold for T255-34 and 2000-fold for 3718.v3.c11). The enhanced neutralization activity of NIH45-46G54W implies that Trp54 forms a favorable hydrophobic interaction with Phe43 cavity of gp120 as seen in VRC03-gp120 (PDB 3SE8). NIH45-46G54F showed some increased activity (Tables 6, 7 and 8). Substituting Gly54 with tryptophan adds about 140 Å2 of buried surface area on VH when complexed with gp120, and is consistent with the reduced dissociation rates observed in surface plasmon resonance (SPR) experiments (FIGS. 13a, 13B, and 13C). By providing a tryptophan in the Phe43 cavity of gp120, NIH45-46G54W may use higher affinities and/or slower dissociation rates to overcome incompatible surface variations that render some viruses less sensitive or resistant to its effects.
Heavy chain residue 54 is not conserved in HAADs; in addition to glycine (NIH45-46 and VRC01), residue 54 can be threonine (3BNC60, 3BNC117, 3BNC115; VRC-PG04), tyrosine (12A12), phenylalanine (12A21), or arginine (1B2530 and 1NC9), as reported in Sheid et al., 2011, supra; and Wu et al., 2011, supra. Tryptophan substitution in some HAADs was tested and shown in Table 10, below.







TABLE 10







In vitro neutralization IC50 values (μg/mL)


















3BNC60
3BNC60
3BNC117
3BNC117
3BNC55
3BNC55
12A12
12A12


Virus
Clade
WT
T54W
WT
T54W
WT
T54W
WT
Y54W



















SC422661.8
B
0.1
0.1
0.07
0.07
0.3
0.6
0.2
0.2


AC10.0.29
B
13
3.1
6.5
2.8
>50
>50
0.6
0.5


TRO.11
B
0.07
0.06
0.6
0.6
7.6
>50
0.3
0.2


DU172.17
C
0.05
0.04
0.04
0.9
2
>50
0.2
0.1


CAP210.2.00.E8
C
4.7
5.0
11
2.8
>50
>50
>50
>50


CAP45.2.00.G3
C
10
19
16
23
>50
>50
0.4
0.2









Passive immunization and/or gene therapy to deliver HIV antibodies is increasingly being considered as an option for prevention of HIV infection. To reduce the concentrations and numbers of antibodies required for protection to realistic and affordable levels, highly potent and broadly neutralizing antibodies are the reagents of choice for passive delivery. Although it is difficult to compare the potencies and breadth of antibodies characterized using different virus panels, the natural form of NIH45-46 exhibits superior potency to VRC01 when compared against a panel of 82 Tier 2 and 3 viruses representing all known HIV clades (Sheid et al., 2011, supra). One set of HIV antibodies, the PGT antibodies that recognize the gp120 V3 loop and associated carbohydrates, exhibited median IC50s up to 10-fold lower than VRC01 (Walker et al., 2011, Nature, 477:466-471, the entire contents of which are incorporated herein by reference), but are less potent and broad than NIH45-46G54W (FIG. 15B, and Table 11, below).







TABLE 11





IC50 from PGT antibodies and VRC01 using the same virus panel





























PGT-
PGT-
PGT-
PGT-
PGT-
PGT-
PGT-
PGT-
PGT-
PGT-
PGT-



Isolate
121
122
123
125
126
127
128
130
131
135
136





Include >50
Geometric
0.53
1.03
0.66
1.85
1.05
2.92
0.39
3.07
7.27
9.53
30.39


(μg/mL)
mean



Arithmetic
16.63
19.39
18.29
25.81
21.75
26.19
15.31
25.39
31.06
34.91
44.02



mean



Median
0.31
2.02
0.35
34.97
1.08
42.83
0.10
22.98
50.00
50.00
50.00


Exclude >50
Geometric
0.07
0.13
0.08
0.09
0.08
0.17
0.06
0.24
0.41
0.32
2.25


(μg/mL)
mean



Arithmetic
2.17
3.21
2.44
3.34
2.82
2.39
1.56
3.09
2.80
3.88
12.74



mean



Median
0.03
0.05
0.03
0.04
0.04
0.08
0.02
0.16
0.52
0.17
7.81



% viruses <50
70%
65%
67%
52%
60%
50%
72%
52%
40%
33%
16%



(μg/mL)
























PGT-
PGT-
PGT-
PGT-
PGT-
PGT-

VRC-





Isolate
137
141
142
143
144
145
VRC01
PG04
PG9







Include >50
Geometric
23.53
3.15
2.40
3.14
13.62
0.91
0.45
0.57
1.27



(μg/mL)
mean




Arithmetic
41.22
24.62
23.30
24.62
33.76
12.83
4.41
7.92
15.89




mean




Median
50.00
16.01
9.46
13.76
50.00
0.86
0.34
0.30
0.62



Exclude >50
Geometric
1.68
0.33
0.24
0.34
1.58
0.30
0.32
0.30
0.36



(μg/mL)
mean




Arithmetic
10.51
3.80
2.99
4.32
6.87
2.59
1.04
1.99
4.33




mean




Median
3.46
0.35
0.21
0.31
2.06
0.29
0.32
0.20
0.23




% viruses <50
22%
55%
57%
56%
38%
78%
92%
88%
75%




(μg/mL)










Table 11 above shows a comparison of mean and median IC50 (μg/mL) values for PGT antibodies and VRC01. A direct comparison between NIH45-46 and the PGT antibodies is not available. However, VRC01 (which was shown in a direct comparison to be less potent than NIH45-46) was directly compared to the PGT antibodies using the same virus panel. (Sheid et al., 2011, supra.) Mean IC50 values were calculated using data taken from Sheid et al., 2011, supra. Geometric and arithmetic means were calculated to include data for all viral strains (listed as Include >50, in which case, values reported as IC50>50 μg/mL were entered as 50 μg/mL in the calculation) and to exclude viral strains in which the IC50 was >50 μg/mL (listed as Exclude >50, in which case the percent of viral strains with IC50s<50 μg/mL is also reported). Mean IC50s are compared with the median IC50s as reported in Sheid et al., 2011, supra.
Contacts between the antibody light chain and gp120 are mostly conserved between the NIH45-46-93THO57 and VRC01-93THO57 structures with a notable exception: Ser28NIH45-46 LC in CDRL1 replaces a solvent-exposed tyrosine (Tyr28VRC01 LC) that interacts with ordered N-linked carbohydrate attached to Asn27693TH057. By contrast, the Ser28NIH45-46 LC sidechain does not contact gp120 carbohydrates; instead it faces away from gp120, hydrogen bonding with Arg64NIH45-46 LC (FWR3) and creating a 2.7 Å displacement of the main-chain Cα atoms (FIG. 11A). The Ser28NIH45-46 LC-Arg64NIH45-46 LC interaction is maintained in unbound NIH45-46 (FIG. 11B). The position 28 substitution of serine for tyrosine largely accounts for the burial of more surface area in gp120's interaction with the VRC01 versus NIH45-46 light chain (681 Å2 versus 395 Å2 total buried surface area; 314 Å2 versus 203 Å2 buried surface area on the light chain) (Tables 5A, 5B). The larger contact area for the VRC01 light chain may account for the ability of VRC01, but not NIH45-46, to neutralize the Glade C CAP45.2.00.G3 strain, given that the NIH45-46 heavy chain paired with the VRC01 light chain neutralizes this strain, whereas the VRC01 heavy chain paired with the NIH45-46 light chain does not (Table 12). However, the VRC01 light chain did not increase the potency of NIH45-46 against three other viral strains (Table 12), suggesting that the Tyr28 interaction with gp120 carbohydrate is not obligatory.







TABLE 12







In vitro neutralization IC50 values (μg/mL)















NIH45-
VRC01






46 HC
HC




NIH45-
VRC01
NIH45-


Virus
Clade
46
LC
46 LC
VRC01















AC10.0.29
B
0.9
1.0
4.5
0.8


TRO.11
B
1.9
0.3
24
0.5


SC422661.8
B
0.05
0.2
0.4
0.2


QH0692.42
B
0.7
0.9
1.2
0.7


ZM214M.PL15.11
C
0.5
0.6
1.8
0.8


CAP45.2.00.G3
C
>50
2.1
>50
1.8


T257-31
CRF02
0.5
0.6
15
1.0



(A/G)









Example 3
Protein Expression and Purification
Proteins were produced and purified using previously-described methods (Diskin et al., 2010, Nat. Struct. Mol. Biol., 17:608-613, the entire contents of which are incorporated herein by reference). Briefly, NIH45-46 IgG was expressed by transient transfection in HEK293-6E cells. Secreted IgG was purified from cell supernatants using protein A affinity chromatography (GE Healthcare). Fab fragments were prepared by digesting purified IgG with immobilized papain (Pierce) at 10 mg/mL and then separating Fabs from Fc-containing proteins using protein A chromatography and Superdex 200 16/60 size exclusion chromatography. For crystallization trials, the NIH45-46 Fab for crystallization experiments was concentrated to 11 mg/mL in 20 mM Tris pH 8.0, 150 mM sodium chloride, 0.02% sodium azide (TBS). Substitutions in heavy chain residue 54 of NIH45-46, 3BNC55, 12A12, 3BNC117 and 3BNC60 were introduced using a Quikchange II kit (Agilent technologies). Wild type, mutant forms and chain swapped versions of these proteins were expressed as IgGs in HEK293-6E cells and purified by protein A chromatography as described for NIH45-46 IgG. Proteins were stored at a concentration of 1 mg/mL for neutralization assays in either 10 mM sodium citrate pH 3.05, 50 mM sodium chloride, 0.02% sodium azide or in TBS (12A12 and 12A12Y54W) or in phosphate buffered saline (NIH45-46 mutated/truncated in CDRH3 and NIH45-46/VRC01 heavy and light chain swapped antibodies (Abs)) prior to dilution into neutral pH cell media. For SPR analyses, NIH45-46 and NIH45-46G54W heavy chains were subcloned into the pTT5 (NRC-BRI) expression vector to encode C-terminal 6×-His tagged Fab heavy chains (VH-CH1-6×-His tag), and the heavy chain expression vectors were co-transfected with the appropriate light chain vector into HEK293-6E cells. Supernatants were collected after 7 days, buffer exchanged into TBS and loaded on a Ni2+-NTA affinity column (Qiagen). Fabs were eluted using TBS supplemented with 250 mM imidazole and further purified by Superdex200 10/300 size exclusion chromatography (GE Healthcare) in TBS.
Genes encoding truncated 93TH053, CAP244.2.00.D3, and Q259.d2.17 gp120 cores including the deletions and modifications described in Zhou et al., 2010, supra (the entire contents of which are incorporated herein by reference), were chemically synthesized (BlueHeron). An extra disulfide bond was introduced into 93TH053 by changing the Val65 and Ser115 codons into cysteines.
The modified core genes were subcloned into the pACgp67b expression vector (BD Biosynthesis) to include a C-terminal 6×-His tag, expressed in baculovirus-infected insect cells, and purified from insect cell supernatants as previously described in Diskin et al., 2010, supra. For crystallization experiments, purified NIH45-46 Fab and 93TH057 gp120 were incubated at a 1:1 molar ratio and treated with 40 kU of Endoglycosidase H (New England Biolabs) for 16 hours at 37° C. The complex was purified after the incubation by Superdex 200 10/300 size exclusion chromatography (GE Healthcare) and then concentrated to OD280=9.6 in 20 mM Tris pH 8.0, 300 mM sodium chloride, 0.02% sodium azide.
Example 4
Crystallization
Crystallization screening was done by vapor diffusion in sitting drops by a Mosquito® crystallization robot (TTP labs) using 400 nL drops (1:1 protein to reservoir ratio) utilizing commercially available crystallization screens (Hampton). Initial crystallization hits for Fab NIH45-46 and for NIH45-46-93TH057 complex were identified using the PEGRx HTTM (Hampton) screen and then manually optimized. Thin needle-like crystals of Fab NIH45-46 (space group P212121, a=49.4 Å, b=87.4 Å, c=166.4 Å; one molecule per asymmetric unit) were obtained upon mixing a protein solution at 11 mg/mL with 12% polyethylene glycol 20,000, 0.1 M sodium acetate pH 5.0, 0.1 M sodium/potassium tartrate, 0.02 M ammonium sulfate at 20° C. Crystals were briefly soaked in mother liquor solution supplemented with 15% and then 30% glycerol before flash cooling in liquid nitrogen. Crystals of the NIH45-46-93TH057 complex (space group P212121, a=69.1 Å, b=70.5 Å, c=217.7 Å; one molecule per asymmetric unit) were obtained upon mixing a protein solution at OD280=9.6 with 12% isopropanol, 10% polyethylene glycol 10,000, 0.1 M sodium citrate pH 5.0 at 20° C. Complex crystals were cryo-cooled by covering the crystallization drops with paraffin oil to prevent evaporation and then adding an excess of 20% isopropanol, 5% glycerol, 10% polyethylene glycol, 0.1 M sodium citrate pH 5.0 to the drops prior to mounting and flash cooling the crystals in liquid nitrogen.
Example 5
Data Collection, Structure Solution and Refinement
X-ray diffraction data were collected at the Stanford Synchrotron Radiation Lightsource (SSRL) beamline 12-2 using a Pilatus 6M pixel detector (Dectris). The data were indexed, integrated and scaled using XDS as described in Kabsch, 2010, Acta Crystallogr D Biol Crystallogr, 66:125-132, the entire contents of which are incorporated herein by reference. The Fab NIH45-46 structure was solved by molecular replacement using Phaser as described in McCoy et al., 2007, J. Appl. Cryst., 40:658-674, the entire contents of which are incorporated herein by reference, and the VHVL and CH1CL domains of the VRC01 Fab (PDB code 3NGB) as separate search models. The model was refined to 2.6 Å resolution using an iterative approach involving refinement using the Phenix crystallography package Adams et al., 2010, Acta Crystallogr D Biol Crystallogr, 66:213-221, the entire contents of which are incorporated herein by reference, and manually fitting models into electron density maps using Coot (Emsley et al., 2004, Acta Crystallogr D Biol Crystallogr, 60:2126-2132, the entire contents of which are incorporated herein by reference). The final model (Rwork=18.4%; Rfree=23.8%) includes 3380 protein atoms, 125 water molecules and 37 ligand atoms, including N-Acetylglucosamine, glycerol and a sulfate ion (FIG. 2). 96.5%, 3.3% and 0.2% of the residues were in the favored, allowed and disallowed regions, respectively, of the Ramachandran plot. The first glutamine of the NIH45-46 heavy chain was modeled as 5-pyrrolidone-2-carboxylic acid.
A search model for solving the NIH45-46-93TH057 complex was created by superimposing the refined structure of the NIH45-46 Fab on the VRC01 Fab in the structure of VRC01-93TH057 (PDB code 3NGB). A molecular replacement solution was found as described above using separate search models for the VHVL domains of NIH45-46 complexed with 93TH057 and the CH1CL domains of NIH45-46. (FIG. 2). The complex structure was refined to 2.45 Å resolution as described for the Fab structure. To reduce model bias, the CDRH3 of NIH45-46 was omitted from the model and then built into electron density maps after a few rounds of refinement.
The final model (Rwork=20.7%; Rfree=25.6%) includes 5989 protein atoms, 67 water molecules and 148 atoms of carbohydrates, citrate and chloride ions (FIG. 2). 96.1%, 3.5% and 0.4% of the residues were in the favored, allowed and disallowed regions, respectively, of the Ramachandran plot. Disordered residues that were not included in the model were residues 1-2 of the NIH45-46 light chain, residues 133-136 and 219-221 of the heavy chain, and residues 302-308 (V3 substitution), residues 397-408 (a total of 6 residues from V4) and the 6×-His tag of 93TH057. The first glutamine of the NIH45-46 heavy chain was modeled as 5-pyrrolidone-2-carboxylic acid.
Buried surface areas were calculated using AreaIMol in CCP4 and a 1.4 Å probe. Superimposition calculations were done and molecular representations were generated using PyMol (The PyMOL Molecular Graphics System, Schrödinger, LLC).
Example 6
Surface Plasmon Resonance (SPR) Measurements
The binding of gp120 core proteins to wild-type NIH45-46 Fab and to mutant (NIH45-46G54W) Fab was compared using a Biacore T100 instrument (GE Healthcare). Purified NIH45-46 and NIH45-46G54W Fabs were immobilized at coupling densities of 500 resonance units (RU) or 1500 RU on a CM5 sensor chip (Biacore) in 10 mM acetate pH 5.0 using primary amine coupling chemistry as described in the Biacore manual. One of the four flow cells on each sensor chip was mock-coupled using buffer to serve as a blank. Experiments were performed at 25° C. in 20 mM HEPES, pH 7.0, 150 mM sodium chloride and 0.005% (v/v) surfactant P20, and the sensor chips were regenerated using 10 mM glycine, pH 2.5. gp120 core proteins were injected in a two-fold dilution series at concentrations ranging from 500 nM to 31.2 nM at a flow rate of 70 μL/min. After subtracting the signal from the mock-coupled flow cell, kinetic data were globally fit to a 1:1 binding model (Biacore evaluation software) to derive on- and off-rate constants, which were used to calculate affinities as KD=kd/ka.
Example 7
In Vitro Neutralization Assays
A previously-described pseudovirus neutralization assay was used to compare the neutralization potencies of wild-type and mutant IgGs as previously described in Montefiori, 2005, Current protocols in immunology, Edited by John E. Coligan et al., Chapter 12, Unit 12.11, the entire contents of which are incorporated herein by reference. Briefly, pseudoviruses were generated in HEK293T cells by co-transfection of an Env-expressing vector and a replication-incompetent backbone plasmid. Neutralization was assessed by measuring the reduction in luciferase reporter gene expression in the presence of a potential inhibitor following a single round of pseudovirus infection in TZM-bl cells. Antibodies were pre-incubated with 250 infectious viral units in a three or four-fold dilution series for one hour at 37° C. before adding 10,000 TZM-bl cells per well for a two-day incubation. Cells were then lysed and luciferase expression was measured using BrightGlo (Promega) and a Victor3 luminometer (PerkinElmer). Nonlinear regression analysis using the program Prism (GraphPad) was used to calculate the concentrations at which half-maximal inhibition was observed (IC50 values) as described in Klein et al., 2009, PNAS, 106:7385-7390, the entire contents of which are incorporated herein by reference. Samples were initially screened in duplicates. Reagents that showed enhanced activity were tested again as triplicates. Values for NIH45-46 and NIH45-46G54W in FIG. 14 were obtained from three independent experiments. Similar IC50 values were obtained in two independent neutralization experiments using different dilution series.
Example 8
Signature Features of PVL Antibodies
The correlation between neutralization potency and the length of two of the light chain CDR loops was analyzed in CD4bs antibodies. The relatively small CDRL1 of VRC01, which has a 2-residue deletion relative to its germline precursor, was previously correlated with increased neutralization potency (Zhou et al., 2010, supra). It was noted that sequences of VRC01, NIH45-46, and VRC-PG04 revealed a more striking correlation for the length of CDRL3, which is only 5 residues in these antibodies. Examination of the large Abysis database for human Ab sequences (http://www.bioinf.org.uk/abs/) showed that only about 1% of VL domains have a CDRL3 length of 5 amino acids, compared with more typical 9-11 residue lengths. Larger CDRL3 loops would place critical side chains at the tip of CDRL3 in different locations, thus not able to interact with gp120 in the same manner. In antibodies with longer CDRL3s, the tip of CDRL3 interacts with Trp47HC, a highly conserved residue (found in 63 of 69 germline VH gene segments) that plays a similar role as Trp102HC in the Abs with 5-residue CDRL3s to stabilize the VH-VL interface.
V domain alignments revealed the following sequence characteristics of the most potent of the VRC01-like Abs: complete conservation of heavy chain Arg71HC, Trp50HC, Asn58HC, and Trp102HC, and light chain Glu90LC, Trp65LC/Phe65LC and a CDRL3 length of exactly 5 amino acids (residues are numbered here as in the structure of NIH45-46; pdb code 3U7Y). Analysis of the per residue variability of VH1-2*02-derived Abs indicates that the conservation of Trp50HC and Asn58HC is unlikely to be coincidental.
The roles that conserved residues play in the VH domain structure and in binding to the CD4bs on gp120 are shown schematically in FIG. 16 and Table 13, below. The figures are based on interactions present in the gp120 complexes of VRC01, NIH45-46, and VRC-PG04 (Wu et al., 2011, Science, 333:1593-1602; Diskin et al., 2011, Science, 334:1289-1293; and Zhou et al., 2010, Science, 329:811-817, the entire contents of all of which are incorporated herein by reference.







TABLE 13







PVL Features








PVL Characteristic feature
Role





Trp50HC
H bond with Asn280gp120


Asn58HC
H bond with Arg456gp120


Arg71HC
H bond/salt bridge with Asp368gp120


Trp102*HC
H bond with Asn/Asp279gp120


Glu90**LC
H bond with Gly459gp120


Trp65***LC/
interaction with Asn276gp120 glycan


Phe65***LC


5-residue CDRL3
prevent steric clashes, position 89LC &



90LC side chains





*Position Trp100B;


**Postion Glu96; and


***Trp67/Phe67 using Kabat numbering system.






The side chains of Trp50HC, Trp102HC, and Trp47HC form an unusual propeller-like arrangement on the surface the VH domain. (Although Trp47HC participates in the “propeller,” it is not considered to be a signature residue of potent CD4bs antibodies because it is commonly found in VH domains.) The main interactions of the characteristic VH domain residues with gp120 are as follows: Trp50HC: indole N—H hydrogen bonds with the side chain oxygen of Asn280gp120; Asn58HC: side chain N—H hydrogen bonds with the backbone carbonyl of Arg456gp120; Arg71HC: side chain hydrogen bonds/salt bridges with the side chain of Asp368gp120; and Trp102HC: indole N—H hydrogen bonds with the side chain oxygen of Asn/Asp279gp120. Trp102HC also buries 85 Å2 of surface area at the VH/VL interface—contacting residues Tyr89Lc and Glu90LC.
In the light chains, the side chain of Glu90LC forms a hydrogen bond with the backbone nitrogen of Gly459gp120 and/or the side chain of Asn280gp120. The conservation of Trp65LC/Phe65LC is surprising as this position is distant from gp120 in the available crystal structures.
For those interactions that depend on specific gp120 side chains, the degree of conservation of the relevant gp120 residues is 96.4% for Asn/Asp279gp120, 96.4% for Asn280gp120, and 99.7% for Asp368gp120 (based on the 2010 filtered web alignment of 2869 HIV-1 sequences in the Los Alamos HIV database; http://www.hiv.lanl.gov/). Arg456gp120, which is involved in a main-chain hydrogen bond with the sidechain of Asn58HC, is also highly conserved (95.0%).
An SPR-based binding assay demonstrated detectable binding of the germline heavy chain/mature light chain IgG to immobilized gp140 trimers. Binding of germline heavy chain IgGs was analyzed with substitutions in the four signature heavy chain residues (W50S, N58S, R71T, and W102S) (again paired with the mature 3BNC60 light chain). The W50S, R71T, and W102S mutants showed little or no gp140 binding, and the N58S mutation diminished binding by about 20-fold, consistent with the corresponding PVL characteristic residues playing key roles in recognition of the HIV-1 envelope spike by the germline PVL B cell receptor.
To examine the importance of the signature PVL residues to their activity, the gp120 sequences of HIV-1 strains resistant to neutralization by NIH45-46 were analyzed. The gp120 residue variants associated with resistance were identified by three criteria: first, they are contact residues with NIH45-46; second, they are absent in NIH45-46-resistant viruses; third, they do not appear in NIH45-46-sensitive viruses. The critical positions identified were 279gp120, 280gp120, 456gp120, 458gp120, and 459gp120; the common (i.e., sensitive) residues at these positions are Asx, Asn, Arg, Gly, and Gly, respectively, where Asx is Asp or Asn. These sites make significant contacts with the characteristic PVL residues (FIG. 16). Viral stains with variations at these sites are generally neutralized poorly by all PVL antibodies, as expected if substitutions at these positions interfere with common interactions.
To verify the significance of gp120 variations at these positions, point mutants within the gp160 gene of HIV-1 strain YU2 were engineered, created pseudoviruses carrying the mutant gp160s, and determined the neutralization potencies of the PVL NIH45-46 (Diskin et al., 2011, supra) (as characterized by IC50 values). Mutations at 279gp120 and 280gp120 rendered the virus resistant to neutralization by NIH45-46G54W, and substitution of 458gp120 diminished the neutralization potency by >1500-fold (FIG. 17).
As disclosed throughout and evidenced by, for example, the neutralization assays of FIG. 14, a PVL antibody such as NIH45-46, having a hydrophobic amino acid (Trp) substituted at the Phe43CD4-equivalent residue (position 54) has increased potency and breadth in HIV strains. Furthermore, methods are provided for identifying a PVL antibody and the Phe43CD4-equivalent residue for substitution with a hydrophobic amino acid.
While the present invention has been illustrated and described with reference to certain exemplary embodiments, those of ordinary skill in the art will understand that various modifications and changes may be made to the described embodiments without departing from the spirit and scope of the present invention, as defined in the following claims.

## Claims

1. A composition comprising an isolated human anti-CD4 binding site (anti-CD4bs) antibody variant having a light chain and a heavy chain, the heavy chain comprising an amino acid (X) substitution at position 54 selected from G54X, T54X, or S54X according to Kabat numbering, the amino acid (X) being selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, tryptophan, tyrosine, valine, histidine, arginine, glutamine, asparagine, lysine, glutamic acid, and aspartic acid, the anti-CD4bs antibody variant comprising a variant group selected from the group consisting of a VRC01 related variant group, a 3BNC117 related variant group, a VRC-PG04 related variant group, a CH31 related variant group, and a 12A12 related variant group,
the VRC01 related variant group comprising:
a complementarity determining region (CDR) 1 of the heavy chain (CDRH1) having a sequence of Gly26-Tyr27-Glu28-Phe29-(lle/Leu)30-(Asn/Asp)31-Cys32 as set forth in SEQ ID NOs: 2 and 6;
a CDR 2 of the heavy chain (CDRH2) having a sequence of SEQ ID NO: 74 Lys52-Pro52A-Arg53-Gly54-Gly55-Ala56 as set forth in SEQ ID NOs: 2 and 6;
a CDR 3 of the heavy chain (CDRH3) having a sequence selected from:
Gly95-Lys96-(Asn/Tyr)97-Cys98-(Asp/Thr)99-Tyr100-Asn100A-Trp100B-Asp100C-Phe100D-Glu101-His102 as set forth in SEQ ID NO: 2 or Gly95-Lys96-(Asn/Tyr)97-Cys98-(Asp/Thr)99-Ala100-Arg100A-Asp100B-Tyr100C-Tyr100D-Asn100E-Tryp100F-Asp100G-Phe100H-Glu101-His102 as set forth in SEQ ID NO: 6;

a CDR 1 of the light chain (CDRL1) having a sequence of Arg24-Thr25-Ser26-Gln27-(Ser/Tyr)28-Gly29-Ser30-Leu33-Ala34 as set forth in SEQ ID NOs: 1 and 5;
a CDR 2 of the light chain (CDRL2) having a sequence of Ser50-Gly51-Ser52-Thr53-Arg54-Ala55-Ala56 as set forth in SEQ ID NOs: 1 and 5; and
a CDR 3 of the light chain (CDRL3) having a sequence of Gln89-Gln90-Tyr91-Glu96-Phe97 as set forth in SEQ ID NOs: 1 and 5,

the 3BNC117 related variant group comprising:
a CDRH1 having a sequence of Gly26-Tyr27-(Asn/Glu/Lys)28-lle29-(Arg/Ser)30-Asp31-(His/Tyr)32 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRH2 having a sequence of Asn52-Pro52A-Lys53-Thr54-Gly55-Gln56 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRH3 having a sequence of Gln95-Arg96-Ser97-Asp98-(Phe/Tyr)100A-Trp100B-Asp100C-Phe100D-Asp101-Val102 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRL1 having a sequence of Gln24-Ala25-Asn26-Gly27-Tyr28-Leu33-Asn34 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15;
a CDRL2 having a sequence of Asp50-Gly51-Ser52-Lys53-Leu54-Glu55-(Arg/Thr)56 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15; and
a CDRL3 having a sequence of Gln89-Val90-Tyr91-Glu96-Phe97 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15,

the VRC-PG04 related variant group comprising:
a CDRH1 having a sequence of Glu26-Asp27-lle28-Phe29-Glu30-Arg31-Thr32-Glu33 as set forth in SEQ ID NOs: 20, 46, and 59;
a CDRH2 having a sequence of Lys52-(Ala/Thr)52A-Val53-(Ser/Thr)54-Gly55-Ala56 as set forth in SEQ ID NOs: 20, 46, and 59;
a CDRH3 having a sequence selected from the group of Gln95-Lys96-Phe97-Tyr98-(Ala/Thr)99-Gly100-Gly100A-Gln100B-Gly100C-Trp100D-Tyr100E-Phe100F-Asp101-Leu102 as set forth in SEQ ID NOs: 20 and 46 Gln95-Lys96-Phe97-Glu98-Ser99-Arg100-Tyr100A-(Ala/Thr)100B-Gly100C-Gly100D-Gln100E-Gly100F-Trp100G-Tyr100H-Phe100l-Asp101-Leu102 as set forth in SEQ ID NO: 59;
a CDRL1 having a sequence of Thr24-Ala25-Ala26-Ser27-Tyr28-Gly29-His30-Met33-Thr34 as set forth in SEQ ID NO: 19;
a CDRL2 having a sequence of Ala50-Thr51-Ser52-Lys53-Arg54-Ala55-Ser56 as set forth in SEQ ID NO: 19; and
a CDRL3 having a sequence of Gln89-Gln90-Leu91-Glu96-Phe97,

the CH31 related variant group comprising as set forth in SEQ ID NO: 19:
a CDRH1 having a sequence of (Asp/Glu26)-(Ala/Asp)27-Asp28-Asp28A -(Phe/Trp/Tyr)28B-Ser28C-Pro28D-(His/Tyr)28E-Trp28F-(Met/Val)28G-Asn28H-Pro29-Ala30-Pro31-Glu32-His33 as set forth in SEQ ID NOs: 22, 24, 26, 28, and 30;
a CDRH2 having a sequence of (Asn/Lys)52-Pro52A-Thr53-Asn54-Gly55-Ala56 as set forth in SEQ ID NOs: 22, 24, 26, 28, and 30;
a CDRH3 having a sequence of Ala95-Gln96-Lys97-Arg98-(Ala/Gly)99-Arg100-Ser100A-Glu100B-Trp100C-Ala100D-Tyr100E-Ala101-His102 as set forth in SEQ ID NOs: 22, 24, 26, 28, and 30;
a CDRL1 having a sequence of Gln24-Ala25-Ser26-Arg27-Gly28-lle29-Gly30-Lys31-Asp32-Leu33-Asn34 as set forth in SEQ ID NOs: 21, 23, 25, 27, and 29;
a CDRL2 having a sequence of Asp50-Ala51-Ser52-(lle/Thr/Val)53-Leu54-Glu55-Gly56 as set forth in SEQ ID NOs: 21, 23, 25, 27, and 29; and
a CDRL3 having a sequence of Gln89-Gln90-Tyr91-Glu96-Thr97 as set forth in SEQ ID NOs: 21, 23, 25, 27, and 29, and

the 12A12 related variant group comprising:
a CDRH1 having a sequence of Gly26-Tyr27-Ser28-Phe29-Thr30-Asp31-Tyr32 as set forth in SEQ ID NO: 18;
a CDRH2 having a sequence of Lys52-Pro52A-Val53-Tyr54-Gly55-Ala56 as set forth in SEQ ID NO: 18;
a CDRH3 having a sequence of Asp95-Gly96-Ser97-Gly98-Asp99-Asp100-Thr100A-Ser100B-Trp100C-His100D-Leu100E-Asp101-Pro102 as set forth in SEQ ID NO: 18;
a CDRL1 having a sequence of Gln24-Ala25-Gly26-Gln27-Gly28-lle29-Gly30-Ser31-Ser32-Leu33-Gln34 as set forth in SEQ ID NO: 17;
a CDRL2 having a sequence of Gly50-Ala51-Ser52-Asn53-Leu54-His55-Arg56 as set forth in SEQ ID NO: 17; and
a CDRL3 having a sequence of Ala89-Val90-Leu91-Glu96-Phe97 as set forth in SEQ ID NO: 17,


wherein the CDRs are defined according to the Chothia definition.

2. The composition of claim 1, wherein the heavy chain substitution is tryptophan, tyrosine, phenylalanine, histidine, arginine, glutamine, or asparagine.

3. The composition of claim 1, wherein the human anti-CD4bs antibody is capable of binding to gp120 at positions corresponding to 279, 280, 368, 458, and 459 according to pdb code 3U7Y.

4. A pharmaceutical composition comprising the human anti-CD4bs antibody of claim 1 or a fragment thereof, and a pharmaceutically acceptable carrier.

5. A method of inhibiting an HIV infection or an HIV-related disease, comprising:
administering a therapeutically effective amount of the composition of claim 1 to a patient.

6. A composition comprising an isolated human anti-CD4 binding site (anti-CD4bs) antibody variant having a light chain and a heavy chain, the heavy chain comprising an amino acid (X) substitution at position 54 selected from G54X, T54X, or S54X according to Kabat numbering, the amino acid (X) being selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, tryptophan, tyrosine, valine, histidine, arginine, glutamine, asparagine, lysine, glutamic acid, and aspartic acid, the anti-CD4bs antibody variant comprising a variant group selected from the group consisting of a VRC01 related variant group and a 3BNC117 related variant group,
the VRC01 related variant group comprising:
a complementarity determining region (CDR) 1 of the heavy chain (CDRH1) having a sequence of Gly26-Tyr27-Glu28-Phe29-(lle/Leu)30-(Asn/Asp)31-Cys32 as set forth in SEQ ID NOs: 2 and 6;
a CDR 2 of the heavy chain (CDRH2) having a sequence of Lys52-Pro52A-Arg53-Gly54-Gly55-Ala56 as set forth in SEQ ID NOs: 2 and 6;
a CDR 3 of the heavy chain (CDRH3) having a sequence selected from:
Gly95-Lys96-(Asn/Tyr)97-Cys98-(Asp/Thr)99-Tyr100-Asn100A -Trp100B-Asp100C-Phe100D-Glu101-His102 as set forth in SEQ ID NO: 2 or Gly95-Lys96-(Asn/Tyr)97-Cys98-(Asp/Thr)99-Ala100-Arg100A-Asp100B-Tyr100C-Tyr100D-Asn100E-Tryp100F-Asp100G-Phe100H-Glu101-His102 as set forth in SEQ ID NO: 6;

a CDR 1 of the light chain (CDRL1) having a sequence of Arg24-Thr25-Ser26-Gln27-(Ser/Tyr)28-Gly29-Ser30-Leu33-Ala34 as set forth in SEQ ID NOs: 1 and 5;
a CDR 2 of the light chain (CDRL2) having a sequence of Ser50-Gly51-Ser52-Thr53-Arg54-Ala55-Ala56 as set forth in SEQ ID NOs: 1 and 5; and
a CDR 3 of the light chain (CDRL3) having a sequence of Gln89-Gln90-Tyr91-Glu96-Phe97 as set forth in SEQ ID NOs: 1 and 5, and

the 3BNC117 related variant group comprising:
a CDRH1 having a sequence of Gly26-Tyr27-(Asn/Glu/Lys)28-lle29-(Arg/Ser)30-Asp31-(His/Tyr)32 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRH2 having a sequence of Asn52-Pro52A-Lys53-Thr54-Gly55-Gln56 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRH3 having a sequence of Gln95-Arg96-Ser97-Asp98-(Phe/Tyr)100A-Trp100B-Asp100C-Phe100D-Asp101-Val102 as set forth in SEQ ID NOs: 8, 10, 12, 14, and 16;
a CDRL1 having a sequence of Gln24-Ala25-Asn26-Gly27-Tyr28-Leu33-Asn34 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15;
a CDRL2 having a sequence of Asp50-Gly51-Ser52-Lys53-Leu54-Glu55-(Arg/Thr)56 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15; and
a CDRL3 having a sequence of Gln89-Val90-Tyr91-Glu96-Phe97 as set forth in SEQ ID NOs: 7, 9, 11, 13, and 15,


wherein the CDRs are defined according to the Chothia definition.

7. The composition of claim 6, wherein the heavy chain substitution is tryptophan, tyrosine, phenylalanine, histidine, arginine, glutamine, or asparagine.

8. The composition of claim 6, wherein the human anti-CD4bs antibody is capable of binding to gp120 at positions corresponding to 279, 280, 368, 458, and 459 according to pdb code 3U7Y.

9. A pharmaceutical composition comprising the human anti-CD4bs antibody of claim 6 or a fragment thereof, and a pharmaceutically acceptable carrier.

10. A method of inhibiting an HIV infection or an HIV-related disease, comprising:
administering a therapeutically effective amount of the composition of claim 6 to a patient.