# Patent

## Title
Active compound combinations

## Abstract

The invention relates to active compound combinations, in particular a fungicidal and/or insecticidal composition, comprising Isotianil (3,4-dichloro-N-(2-cyanophenyl)-5-isothiazolecarboxamide) and at least one further insecticide of the tetronic acid derivatives group and optionally one further insecticide of the neonicotinoids.
Moreover, the invention relates to a method for curatively or preventively controlling the phytopathogenic fungi and/or microorganisms and/or pests of plants or crops, to the use of a combination according to the invention for the treatment of seed, to a method for protecting a seed and not at least to the treated seed.

## Background
The invention relates to active compound combinations, in particular a fungicidal and/or insecticidal composition, comprising Isotianil (3,4-dichloro-N-(2-cyanophenyl)-5-isothiazolecarboxamide, CAS No 224049-04-1) and at least one further insecticide of the group of cyclic ketoenols and optionally one further insecticide of the neonicotinoids.
Moreover, the invention relates to a method for curatively or preventively controlling the phytopathogenic fungi and/or microorganisms and/or pests of plants or crops, to the use of a combination according to the invention for the treatment of seed, to a method for protecting a seed and not at least to the treated seed.
It is already known that the compound (A) Isotianil has fungicidal and insecticidal properties. In addition, it has also been found that the isothiazolecarboxylic acid derivatives are highly suitable for protecting plants against attack by undesirable phytopathogenic fungi and microorganisms (U.S. Pat. No. 5,240,951 and JP-A 06-009313). The compound (A) Isotianil according to the invention is suitable both for mobilizing the defenses of the plant against attack by undesirable phytopathogenic fungi and microorganisms and as microbicide for the direct control of phytopathogenic fungi and microorganisms. In addition, the compound (A) is also active against pests which damage plants (WO 99/24414). The activity of this substance is good; however, at low application rates it is in some cases unsatisfactory.
Furthermore, it is already known that neonicotinoids according to group (C) can be used for controlling pests of plant and crops (Pesticide Manual, 14th. Edition (2006); “Modern Agrochemicals”, Vol. 4, No. 3, June 2005; EP-A 0 428 941) and that the compounds of group (B) are also suitable for controlling pests of plants and crops (DE-A 4216814 and WO-A 98/005638). However, the activity of these substances at low application rates is likewise not always sufficient. In addition, also the binary combinations of neonicotinoids according to group (C) and compounds from group (B) are known (WO-A 2001/24634, WO-A 2000/56156, WO-A 2001/89300, WO-A 2008/006516).
It is also known that the combination of Isotianil with neonicotinoids is suitable for controlling phytopathogenic fungi (WO 2005/009131). A fungicidal and insecticidal composition comprising Isotianil and fipronil or ethiprole, optionally together with another insecticide or fungicide, is known from WO 2008/046533. Ternary active compound combinations comprising Isotianil, imidacloprid, and one further insecticidally or fungicidally active compound are known from WO 2009/015763. WO 2009/074235 relates to active compound combinations comprising Isotianil, at least one further insecticide selected from ethiprole and fipronil, and one further insecticide of the neonicotinoids. The biological profile of spirotetramat is known from Bayer CropScience Journal 61(2), 245-277, 2008. This article is, however, silent on the possibility of employing spirotetramat in combination with further insecticides and/or fungicides. Resistance management guidelines for spirotetramat were published in Bayer CropScience Journal 61(2), 403-416, 2008. The article shortly mentions that the use of spirotetramat in tank mixes or ready formulations with neonicotinoids or pyrethroids should be restricted. However, the article does not disclose a synergistic effect of such tank mixes or ready formulations nor does it mention isotianil as further combination partner. The effect of spiromesifen, among other insecticides, against one specific pest in a tea field was published in Chagyo Kenkyu Hokoku 101, 25-28, 2006. This article is, however, also silent on the possibility of employing spiromesifen in combination with further insecticides and/or fungicides.
Fungicidal and/or insecticidal compositions, comprising Isotianil and at least one further insecticide of the group of cyclic ketoenols and optionally one further insecticide of the neonicotinoids are not disclosed in the prior art, nor is there any hint that would point to a synergistic activity of such combinations.
Since, moreover, the environmental and economic requirements imposed on modern-day fungicides are continually increasing, with regard, for example, to the spectrum of action, toxicity, selectivity, application rate, formation of residues, and favorable preparation ability, and since, furthermore, there may be problems, for example, with resistances developing to known active compounds, a constant task is to develop new fungicide and insecticide agents which in some areas at least have advantages over their known counterparts.
The invention provides active compound combinations/compositions which in some aspects at least achieve the stated objectives.
It has now been found, surprisingly, that the combinations according to the invention not only bring about the additive enhancement of the spectrum of action with respect to the phytopathogenic fungi and/or microorganisms and/or pests to be controlled but achieve a synergistic effect which extends the range of action of the compound (A), (B) and (C) in two ways. Firstly, the rates of application of the compounds (A), (B) and (C) are lowered whilst the action remains equally good. Secondly, the combination still achieves a high degree of phytopathogen control even where the three individual compounds have become totally ineffective in such a low application rate range. This allows, on the one hand, a substantial broadening of the spectrum of phytopathogens that can be controlled and, on the other hand, increased safety in use.
However, besides the actual synergistic action with respect to fungicidal and/or insecticidal activity, the combinations according to the invention also have further surprising advantageous properties which can also be described, in a wider sense, as synergistic activity. Examples of such advantageous properties that may be mentioned are: a broadening of the spectrum of fungicidal and/or insecticidal activity to other phytopathogenic fungi and/or microorganisms and/or pests, for example to resistant strains; a reduction in the rate of application of the active ingredients; adequate pest control with the aid of the compositions according to the invention, even at a rate of application at which the individual compounds are totally ineffective; advantageous behavior during formulation or upon application, for example upon grinding, sieving, emulsifying, dissolving or dispensing; increased storage stability; improved stability to light; more advantageous degradability; improved toxicological or ecotoxicological behavior; improved characteristics of the useful plants including: emergence, crop yields, more developed root system, tillering increase, increase in plant height, bigger leaf blade, less dead basal leaves, stronger tillers, greener leaf color, less fertilizers needed, less seeds or plant propagation material needed, more productive tillers, earlier flowering, early grain maturity, less plant verse (lodging), increased shoot growth, improved plant vigor, and early germination; or any other advantages familiar to a person skilled in the art.
The combination according to the invention can also provide an improved systemicity to the active compounds that are used. Indeed, even if some of the used fungicide compound does not possess any or a satisfying systemicity, within the composition according to the invention these compounds can exhibit such a property.
In a similar manner, the combination according to the invention can allow an increased persistence of the fungicide efficacy of the active compounds that are employed.
Another advantage of the combination according to the invention relies in that an increased efficacy is achievable.
Accordingly, the present invention provides an active compound combination comprising (A) Isotianil and (B) a further insecticidal active compound selected from the group consisting of spirotetramat (CasNo. 203313-25-1), spirodiclofen (CasNo. 148477-71-8) and spiromesifen (CasNo. 283594-90-1)).
Preferred preference is given to combinations comprising (A) Isotianil and (B) an insecticidal active compound spirotetramat.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) an insecticidal active compound spirodiclofen.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) an insecticidal active compound spiromesifen.
In addition, the present invention provides an active compound combination comprising compound (A) Isotianil and
(B) a further insecticidal active compound selected from the group consisting of spirotetramat, spirodiclofen and spiromesifen
(C) and a further insecticidal active compound selected from the group consisting of neonicotinoids, for example imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, imidaclothiz, nitenpyram, dinotefuran, and 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preference is given to combinations comprising (A) Isotianil and (B) spirotetramat and (C) a further insecticidal active compound selected from the group consisting of neonicotinoids, for example imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, imidaclothiz, nitenpyram, dinotefuran, and 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) a further insecticidal active compound selected from the group consisting of neonicotinoids, for example imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, imidaclothiz, nitenpyram, dinotefuran, and 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) a further insecticidal active compound selected from the group consisting of neonicotinoids, for example imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, imidaclothiz, nitenpyram, dinotefuran, and 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) imidacloprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) acetamiprid.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spirotetramat and (C) clothianidin.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) thiacloprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) thiamethoxam.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) imidaclothiz.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) nitenpyram.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirotetramat and (C) dinotefuran.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spirotetramat and (C) 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spirodiclofen and (C) imidacloprid.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spirodiclofen and (C) acetamiprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) clothianidin.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B spirodiclofen and (C) thiacloprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) thiamethoxam.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) imidaclothiz.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen e and (C) nitenpyram.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) dinotefuran.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spirodiclofen and (C) 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spiromesifen and (C) imidacloprid.
Preferred preference is given to combinations comprising compound compound (A) Isotianil and (B) spiromesifen and (C) acetamiprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) clothianidin.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B spiromesifen and (C) thiacloprid.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) thiamethoxam.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) imidaclothiz.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) nitenpyram.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) dinotefuran.
Preferred preference is given to combinations comprising compound (A) Isotianil and (B) spiromesifen and (C) 1-[(2-chloro-5-thiazolyl)methyl]tetrahydro-3,5-dimethyl-N-nitro-1,3,5-triazin-2(1H)-imine.
If the active compounds in the active compound binary combinations according to the invention are present in certain weight ratios, the synergistic effect is particularly pronounced. However, the weight ratios of the active compounds in the active compound combinations can be varied within a relatively wide range. In general, in the combinations according to the invention the compounds (A) and (B) are present in a synergistically effective weight ratio of (A):(B) in a range of 125:1 to 1:125, preferably in a weight ratio of 50:1 to 1:50, more preferably in a weight ratio of 20:1 to 1:20, and most preferably in a weight ratio of 10:1 to 1:10. Further ratios of (A):(B) which can be used according to the present invention with increasing preference in the order given are: 1000:1 to 1:1000, 750:1 to 1:750, 500:1 to 1:500, 400:1 to 1:400, 300:1 to 1:300, 250:1 to 1:250, 200:1 to 1:250, 175:1 to 1:175, 150:1 to 1:150, 125:1 to 1:125, 100:1 to 1:100, 90:1 to 1:90, 80:1 to 1:80, 70:1 to 1:70, 60:1 to 1:60, 40:1 to 1:40, 30:1 to 1:30, 10:1 to 1:10, 5:1 to 1:5, 4:1 to 1:4, 3:1 to 1:3, and 2:1 to 1:2.
For the ternary mixtures the weight ratio of active ingredient compounds is selected as to give the desired, for example synergistic, action. In general, the weight ratio would vary depending on the specific active compound. Generally the weight ratio between any two compounds, independently of each other, is from 125:1 to 1:125, preferably from 75:1 to 1:75, more preferably, 50:1 to 1:50, even more preferably 25:1 to 1:25, and most preferably 10:1 to 1:10.
Further weight ratio between any two compounds, independently of each other, which can be used according to the present invention with increasing preference in the order given are 1000:1 to 1:1000, 750:1 to 1:750, 500:1 to 1:500, 400:1 to 1:400, 300:1 to 1:300, 250:1 to 1:250, 200:1 to 1:250, 175:1 to 1:175, 150:1 to 1:150, 125:1 to 1:125, 100:1 to 1:100, 90:1 to 1:90, 80:1 to 1:80, 70:1 to 1:70, 60:1 to 1:60, 40:1 to 1:40, 30:1 to 1:30, 10:1 to 1:10, 5:1 to 1:5, 4:1 to 1:4, 3:1 to 1:3, 2.1 to 1:2.
Where a compound (A), (B) or (C) can be present in tautomeric form, such a compound is understood hereinabove and herein below also to include, where applicable, corresponding tautomeric forms, even when these are not specifically mentioned in each case.
Compound (A), (B) or (C) having at least one basic centre are capable of forming, for example, acid addition salts, e.g. with strong inorganic acids, such as mineral acids, e.g. perchloric acid, sulfuric acid, nitric acid, nitrous acid, a phosphoric acid or a hydrohalic acid, with strong organic carboxylic acids, such as unsubstituted substituted, e.g. halo-substituted, C1-C4 alkanecarboxylic acids, e.g. acetic acid, saturated or unsaturated dicarboxylic acids, e.g. oxalic, malonic, succinic, maleic, fumaric and phthalic acid, hydroxycarboxylic acids, e.g. ascorbic, lactic, malic, tartaric and citric acid, or benzoic acid, or with organic sulfonic acids, such as unsubstituted or substituted, e.g. halo-substituted, C1-C4 alkane- or aryl-sulfonic acids, e.g. methane- or p-toluene-sulfonic acid. Compound (A), (B) or (C) having at least one acid group are capable of forming, for example, salts with bases, e.g. metal salts, such as alkali metal or alkaline earth metal salts, e.g. sodium, potassium or magnesium salts, or salts with ammonia or an organic amine, such as morpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, e.g. ethyl-, diethyl-, triethyl- or dimethyl-propyl-amine, or a mono-, di- or tri-hydroxy-lower alkylamine, e.g. mono-, di- or tri-ethanolamine. In addition, corresponding internal salts may optionally be formed. In the context of the invention, preference is given to agrochemically advantageous salts. In view of the close relationship between the compound (A), (B) or (C) in free form and in the form of their salts, hereinabove and herein below any reference to the free compound (A), (B) or (C) or to their salts should be understood as including also the corresponding salts or the free compound (A), (B) or (C), respectively, where appropriate and expedient. The equivalent also applies to tautomers of compound (A), (B) or (C) and to their salts.
According to the invention the expression “combination” stands for the various combinations of compounds ((A) and (B)) or ((A) and (B) and (C)), for example in a single “ready-mix” form, in a combined spray mixture composed from separate formulations of the single active compounds, such as a “tank-mix”, and in a combined use of the single active ingredients when applied in a sequential manner, i.e. one after the other within a reasonably short period, such as a few hours or days. Preferably, the order of applying the compounds ((A) and (B)) or ((A) and (B) and (C)) is not essential for working the present invention.
According to the invention the expression “pathogen” stands for all organisms which cause damages on plants or any part of a plant.
According to the invention the expression “fungi” stands for all fungal and chromista organisms.
According to the invention the expression “phytopathogenic fungi” stands for all fungal and chromista organisms which cause damages on plants or any part of a plant. Examples for fungal taxonomic groups are Ascomycota, Basidiomycota, Chytridiomycota, Deuteromycota, Glomeromycota, Microsporidia, Zygomycota, and anamorphic fungi. Examples for Chromista are Oomycota.
According to the invention the expression “microorganisms” stands for all bacterial, viral and protozoan organisms. Examples are Plasmodiophoromycetes.
According to the invention the expression “viruses” stands for all viruses which cause damages on plants or any part of a plant. Examples are DNA-, RNA, and DNA and RNA reverse transcribing viruses as well as subviral agents.
According to the invention the expression “pests” stands for all aschelminthes and panarthropoda organisms which cause damages on plants or any part of a plant. Examples are Nematoda, Arthopoda, Hexapoda and Arachnida.
According to the invention the expression “insecticide” stands for the activity of a compound in combating unwanted insects, acari, or nematodes, or by reducing the damage of plants or plant parts by pests.
The active compounds within the composition according to the invention have potent microbicide activity and can be employed for controlling undesired phytopathogenic fungi and/or microorganisms and/or pests, in crop protection or in the protection of materials.
Within the composition according to the invention, fungicide compounds can be employed in crop protection for example for controlling phytopathogenic fungi and/or microorganisms such as Plasmodiophoromycetes, Oomycota, Chytridiomycota, Zygomycota, Ascomycota, Basidiomycota and Deuteromycota.
Within the composition according to the invention, bactericide compounds can be employed in crop protection for controlling microorganisms for example Pseudomonadaceae, Rhizobiaceae, Enterobacteriaceae, Corynebacteriaceae and Streptomycetaceae.
Within the composition according to the invention, insecticide compounds can be employed in crop protection for example for controlling pests such as lepidoptera. 
The fungicidal and/or insecticidal combination and/or composition according to the invention can be used to curatively or preventively control the phytopathogenic fungi and/or microorganisms and/or pests of plants or crops. Thus, according to a further aspect of the invention, there is provided a method for curatively or preventively controlling the phytopathogenic fungi and/or microorganisms and/or pests of plants or crops comprising the use of a fungicide or insecticide composition according to the invention by application to the seed, the plant or to the fruit of the plant or to the soil in which the plant is growing or in which it is desired to grow.
According to the invention all plants and plant parts can be treated. By plants is meant all plants and plant populations such as desirable and undesirable wild plants, cultivars (including naturally occurring cultivars) and plant varieties (whether or not protectable by plant variety or plant breeder's rights). Cultivars and plant varieties can be plants obtained by conventional propagation and breeding methods which can be assisted or supplemented by one or more biotechnological methods such as by use of double haploids, protoplast fusion, random and directed mutagenesis, molecular or genetic markers or by bioengineering and genetic engineering methods including transgenic plants.
By plant parts is meant all above ground and below ground parts and organs of plants such as shoot, leaf, flower, blossom and root, whereby for example leaves, needles, stems, branches, blossoms, fruiting bodies, fruits and seed as well as roots, corms and rhizomes are listed. Crops and vegetative and generative propagating material, for example cuttings, corms, rhizomes, runners and seeds also belong to plant parts.
According to the invention the expression “plant propagation material” stands for all plant material which can be used either in the vegetative or generative reproduction of plants. Examples for plant propagation material are cuttings, corms, rhizomes, runners, seeds, fruits, grains, pods, fruiting bodies, tubers and seedlings.
The combination/composition according to the invention for combating phytopathogenic fungi and/or microorganisms and/or pests in crop protection comprises an effective, but not phytotoxic amount of the active compounds according to the invention. “Effective, but not phytotoxic amount” is defined as an amount of the combination according to the invention which is sufficient on one hand to control satisfactorily or completely eliminate the fungal disease of the plant and which on the other hand does not lead to any noteworthy symptoms of phytotoxicity. The effective dose can be varied in general in a larger range. The dose is dependent on several factors e.g. the fungi to be combated, the plant, the climatic conditions, and on the active compounds of the combination according to the invention.
Among the plants that can be protected by the method according to the invention, mention may be made of major field crops like corn, soybean, cotton, Brassica oilseeds such as Brassica napus (e.g. canola), Brassica rapa, B. juncea (e.g. mustard) and Brassica carinata, rice, wheat, sugarbeet, sugarcane, oats, rye, barley, millet, triticale, flax, vine and various fruits and vegetables of various botanical taxa such as Rosaceae sp. (for instance pip fruit such as apples and pears, but also stone fruit such as apricots, cherries, almonds and peaches, berry fruits such as strawberries), Ribesioidae sp., Juglandaceae sp., Betulaceae sp., Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae sp., Actinidaceae sp., Lauraceae sp., Musaceae sp. (for instance banana trees and plantings), Rubiaceac sp. (for instance coffee), Theaceae sp., Sterculiceae sp., Rutaceae sp. (for instance lemons, oranges and grapefruit); Solanaceae sp. (for instance tomatoes, potatoes, peppers, eggplant), Liliaceae sp., Compositiae sp. (for instance lettuce, artichoke and chicory—including root chicory, endive or common chicory), Umbelliferae sp. (for instance carrot, parsley, celery and celeriac), Cucurbitaceae sp. (for instance cucumber—including pickling cucumber, squash, watermelon, gourds and melons), Alliaceae sp. (for instance onions and leek), Cruciferae sp. (for instance white cabbage, red cabbage, broccoli, cauliflower, brussel sprouts, pak choi, kohlrabi, radish, horseradish, canola, rapeseed, mustard cress, Chinese cabbage, colza), Leguminosae sp. (for instance peanuts, peas and beans beans—such as climbing beans and broad beans), Chenopodiaceae sp. (for instance mangold, spinach beet, spinach, beetroots), Asteraceae sp. (for instance sunflower), Papilionaceae sp. (for instance soybean), Malvaceae (for instance okra), Asparagaceae (for instance asparagus); horticultural and forest crops; ornamental plants; as well as genetically modified homologues of these crops.
In a preferred embodiment plants that can be protected by the method according to the invention, are Musaceae sp. (for instance banana trees and plantings), Rutaceae sp. (for instance lemons, oranges and grapefruit), corn, soybean, Rosaceae sp. (for instance pip fruit such as apples and pears, but also stone fruit such as apricots, cherries, almonds and peaches, berry fruits such as strawberries), Solanaceae sp. (for instance tomatoes, potatoes, peppers, eggplant), Cucurbitaceae sp. (for instance cucumber—including pickling cucumber, squash, watermelon, gourds and melons), Cruciferae sp. (for instance white cabbage, red cabbage, broccoli, cauliflower, brussel sprouts, pak choi, kohlrabi, radish, horseradish, canola, rapeseed, mustard cress, Chinese cabbage, colza), cotton, Brassica oilseeds such as Brassica napus (e.g. canola), Brassica rapa, B. juncea (e.g. mustard) and Brassica carinata, rice, wheat, sugarbeet, sugarcane, oats, rye, barley, millet, triticale, flax, vine as well as genetically modified homologues of these crops.
In a more preferred embodiment plants that can be protected by the method according to the invention are Musaceae sp. (for instance banana trees and plantings), Rutaceae sp. (for instance lemons, oranges and grapefruit), corn, soybean, rice, wheat, oats, rye, barley, millet, triticale, potatoes, tomatoes, eggplants, Cucurbitaceae sp. (for instance cucumber—including pickling cucumber, squash, watermelon, gourds and melons), cabbage, and strawberries as well as genetically modified homologues of these crops.
The Musaceae family consists, inter alia, of the following species: Musa acuminata, Musa balbisiana, Musa acuminata Colla with the varieties “Dwarf Cavendish”, “Giant Cavendish” and “Gros Michel”, Musa cavendishii Lamb. ex Paxt., Musa malaccensis Ridl., Musa angcorensis Gagnep., Musa aurantiaca, Musa balbisiana, Musa seminifera Lour., Musa banksii F. Muell., Musa basjoo, Musa cheesmanii, Musa flaviflora Simmonds, Musa griersonii, Musa itinerans, Musa laterita, Musa mannii, Musa nagensium, Musa ochracea, Musa ornata Roxb., Musa siamea, Musa sikkimensis, Musa thomsonii Noltie, Musa velutina Wendl. & Drude, Musa alinsanaya, Musa beccarii, Musa boman, Musa borneënsis, Musa bukensis, Musa campestris, Musa coccinea Andrews, Musa uranoscopos Lour, Musa exotica Valmayor, Musa fitzalanii, Musa flavida, Musa gracilis, Musa hirta Becc., Musa insularimontana Hayata, Musa jackeyi, Musa johnsii, Musa lawitiensis, Musa lolodensis, Musa maclayi, Musa monticola, Musa muluensis, Musa paracoccinea, Musa peekelii, Musa pigmaea Hotta, Musa rubra, Musa salaccensis, Musa splendida A. Chev., Musa suratii, Musa textilis: Abacá, Japanese hardy or fibre banana, Musa troglodytarum, Musa tuberculata, Musa violascens, Musa ingens, Musa paradisiaca sapientm, Musa paradisiaca normali, and crosses of these species. The method of treatment according to the invention is used in the treatment of genetically modified organisms (GMOs), e.g. plants or plant propagation material, e.g. seeds or tubers, in particular potato tubers. Genetically modified plants (or transgenic plants) are plants of which a heterologous gene has been stably integrated into the genome. The expression “heterologous gene” essentially means a gene which is provided or assembled outside the plant and when introduced in the nuclear, chloroplastic or mitochondrial genome gives the transformed plant new or improved agronomic or other properties by expressing a protein or polypeptide of interest or by downregulating or silencing other gene(s) which are present in the plant (using for example, antisense technology, co-suppression technology or RNA interference—RNAi-technology). A heterologous gene that is located in the genome is also called a transgene. A transgene that is defined by its particular location in the plant genome is called a transformation or transgenic event.
Depending on the plant species or plant cultivars, their location and growth conditions (soils, climate, vegetation period, diet), the treatment according to the invention may also result in superadditive (“synergistic”) effects. Thus, for example, reduced application rates and/or a widening of the activity spectrum and/or an increase in the activity of the active compounds and compositions which can be used according to the invention, better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, increased flowering performance, easier harvesting, accelerated maturation, higher harvest yields, bigger fruits, larger plant height, greener leaf color, earlier flowering, higher quality and/or a higher nutritional value of the harvested products, higher sugar concentration within the fruits, better storage stability and/or processability of the harvested products are possible, which exceed the effects which were actually to be expected.
Plants and plant cultivars which are preferably to be treated according to the invention include all plants which have genetic material which impart particularly advantageous, useful traits to these plants (whether obtained by breeding and/or biotechnological means).
Plants and plant cultivars which are also preferably to be treated according to the invention are resistant against one or more biotic stresses, i.e. said plants show a better defense against animal and microbial pests, such as against nematodes, insects, mites, phytopathogenic fungi, bacteria, viruses and/or viroids.
Plants and plant cultivars which may also be treated according to the invention are those plants which are resistant to one or more abiotic stresses. Abiotic stress conditions may include, for example, drought, cold temperature exposure, heat exposure, osmotic stress, flooding, increased soil salinity, increased mineral exposure, ozon exposure, high light exposure, limited availability of nitrogen nutrients, limited availability of phosphorus nutrients, shade avoidance.
Plants and plant cultivars which may also be treated according to the invention, are those plants characterized by enhanced yield characteristics. Increased yield in said plants can be the result of, for example, improved plant physiology, growth and development, such as water use efficiency, water retention efficiency, improved nitrogen use, enhanced carbon assimilation, improved photosynthesis, increased germination efficiency and accelerated maturation. Yield can furthermore be affected by improved plant architecture (under stress and non-stress conditions), including but not limited to, early flowering, flowering control for hybrid seed production, seedling vigor, plant size, internode number and distance, root growth, seed size, fruit size, pod size, pod or ear number, seed number per pod or ear, seed mass, enhanced seed filling, reduced seed dispersal, reduced pod dehiscence and lodging resistance. Further yield traits include seed composition, such as carbohydrate content, protein content, oil content and composition, nutritional value, reduction in anti-nutritional compounds, improved processability and better storage stability.
Plants that may be treated according to the invention are hybrid plants that already express the characteristic of heterosis or hybrid vigor which results in generally higher yield, vigor, health and resistance towards biotic and abiotic stress factors. Such plants are typically made by crossing an inbred male-sterile parent line (the female parent) with another inbred male-fertile parent line (the male parent). Hybrid seed is typically harvested from the male sterile plants and sold to growers. Male sterile plants can sometimes (e.g. in corn) be produced by detasseling, i.e. the mechanical removal of the male reproductive organs (or males flowers) but, more typically, male sterility is the result of genetic determinants in the plant genome. In that case, and especially when seed is the desired product to be harvested from the hybrid plants it is typically useful to ensure that male fertility in the hybrid plants is fully restored. This can be accomplished by ensuring that the male parents have appropriate fertility restorer genes which are capable of restoring the male fertility in hybrid plants that contain the genetic determinants responsible for male-sterility. Genetic determinants for male sterility may be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) were for instance described in Brassica species. However, genetic determinants for male sterility can also be located in the nuclear genome. Male sterile plants can also be obtained by plant biotechnology methods such as genetic engineering. A particularly useful means of obtaining male-sterile plants is described in WO 89/10396 in which, for example, a ribonuclease such as barnase is selectively expressed in the tapetum cells in the stamens. Fertility can then be restored by expression in the tapetum cells of a ribonuclease inhibitor such as barstar.
Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may be treated according to the invention are herbicide-tolerant plants, i.e. plants made tolerant to one or more given herbicides. Such plants can be obtained either by genetic transformation, or by selection of plants containing a mutation imparting such herbicide tolerance.
Herbicide-tolerant plants are for example glyphosate-tolerant plants, i.e. plants made tolerant to the herbicide glyphosate or salts thereof. Plants can be made tolerant to glyphosate through different means. For example, glyphosate-tolerant plants can be obtained by transforming the plant with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes are the AroA gene (mutant CT7) of the bacterium Salmonella typhimurium, the CP4 gene of the bacterium Agrobacterium sp, the genes encoding a Petunia EPSPS, a Tomato EPSPS, or an Eleusine EPSPS. It can also be a mutated EPSPS. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate oxido-reductase enzyme. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate acetyl transferase enzyme. Glyphosate-tolerant plants can also be obtained by selecting plants containing naturally-occurring mutations of the above-mentioned genes.
Other herbicide resistant plants are for example plants that are made tolerant to herbicides inhibiting the enzyme glutamine synthase, such as bialaphos, phosphinothricin or glufosinate. Such plants can be obtained by expressing an enzyme detoxifying the herbicide or a mutant glutamine synthase enzyme that is resistant to inhibition. One such efficient detoxifying enzyme is an enzyme encoding a phosphinothricin acetyltransferase (such as the bar or pat protein from Streptomyces species). Plants expressing an exogenous phosphinothricin acetyltransferase are also described.
Further herbicide-tolerant plants are also plants that are made tolerant to the herbicides inhibiting the enzyme hydroxyphenylpyruvatedioxygenase (HPPD). Hydroxyphenylpyruvatedioxygenases are enzymes that catalyze the reaction in which para-hydroxyphenylpyruvate (HPP) is transformed into homogentisate. Plants tolerant to HPPD-inhibitors can be transformed with a gene encoding a naturally-occurring resistant HPPD enzyme, or a gene encoding a mutated HPPD enzyme. Tolerance to HPPD-inhibitors can also be obtained by transforming plants with genes encoding certain enzymes enabling the formation of homogentisate despite the inhibition of the native HPPD enzyme by the HPPD-inhibitor. Tolerance of plants to HPPD inhibitors can also be improved by transforming plants with a gene encoding an enzyme prephenate dehydrogenase in addition to a gene encoding an HPPD-tolerant enzyme.
Still further herbicide resistant plants are plants that are made tolerant to acetolactate synthase (ALS) inhibitors. Known ALS-inhibitors include, for example, sulfonylurea, imidazolinone, triazolopyrimidines, pyrimidinyoxy(thio)benzoates, and/or sulfonylaminocarbonyltriazolinone herbicides. Different mutations in the ALS enzyme (also known as acetohydroxyacid synthase, AHAS) are known to confer tolerance to different herbicides and groups of herbicides. The production of sulfonylurea-tolerant plants and imidazolinone-tolerant plants is describe. Other imidazolinone-tolerant plants are also described. Further sulfonylurea- and imidazolinone-tolerant plants are also described in for example WO 2007/024782.
Other plants tolerant to imidazolinone and/or sulfonylurea can be obtained by induced mutagenesis, selection in cell cultures in the presence of the herbicide or mutation breeding as described for example for soybeans, for rice, for sugar beet, for lettuce, or for sunflower.
Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are insect-resistant transgenic plants, i.e. plants made resistant to attack by certain target insects. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such insect resistance.
An “insect-resistant transgenic plant”, as used herein, includes any plant containing at least one transgene comprising a coding sequence encoding:

    1) an insecticidal crystal protein from Bacillus thuringiensis or an insecticidal portion thereof, such as the insecticidal crystal proteins listed by Crickmore et al., Microbiology and Molecular Biology Reviews (1998), 62, 807-813, updated by Crickmore et al. (2005) at the Bacillus thuringiensis toxin nomenclature, online at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/), or insecticidal portions thereof, e.g., proteins of the Cry protein classes Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Aa, or Cry3Bb or insecticidal portions thereof; or
    2) a crystal protein from Bacillus thuringiensis or a portion thereof which is insecticidal in the presence of a second other crystal protein from Bacillus thuringiensis or a portion thereof, such as the binary toxin made up of the Cry34 and Cry35 crystal proteins; or
    3) a hybrid insecticidal protein comprising parts of different insecticidal crystal proteins from Bacillus thuringiensis, such as a hybrid of the proteins of 1) above or a hybrid of the proteins of 2) above, e.g., the Cry1A.105 protein produced by corn event MON98034; or
    4) a protein of any one of 1) to 3) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes introduced into the encoding DNA during cloning or transformation, such as the Cry3Bb1 protein in corn events MON863 or MON88017, or the Cry3A protein in corn event MIR604;
    5) an insecticidal secreted protein from Bacillus thuringiensis or Bacillus cereus, or an insecticidal portion thereof, such as the vegetative insecticidal (VIP) proteins listed at:
    
        http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html, e.g., proteins from the VIP3Aa protein class; or
    
    
    6) secreted protein from Bacillus thuringiensis or Bacillus cereus which is insecticidal in the presence of a second secreted protein from Bacillus thuringiensis or B. cereus, such as the binary toxin made up of the VIP1A and VIP2A proteins; or
    7) hybrid insecticidal protein comprising parts from different secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as a hybrid of the proteins in 1) above or a hybrid of the proteins in 2) above; or
    8) protein of any one of 1) to 3) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes introduced into the encoding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in cotton event COT102.


Of course, an insect-resistant transgenic plant, as used herein, also includes any plant comprising a combination of genes encoding the proteins of any one of the above classes 1 to 8. In one embodiment, an insect-resistant plant contains more than one transgene encoding a protein of any one of the above classes 1 to 8, to expand the range of target insect species affected when using different proteins directed at different target insect species, or to delay insect resistance development to the plants by using different proteins insecticidal to the same target insect species but having a different mode of action, such as binding to different receptor binding sites in the insect.
Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are tolerant to abiotic stresses. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such stress resistance. Particularly useful stress tolerance plants include:

    a. plants which contain a transgene capable of reducing the expression and/or the activity of poly(ADP-ribose)polymerase (PARP) gene in the plant cells or plants
    b. plants which contain a stress tolerance enhancing transgene capable of reducing the expression and/or the activity of the PARG encoding genes of the plants or plants cells.
    c. plants which contain a stress tolerance enhancing transgene coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway including nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthetase or nicotine amide phosphorybosyltransferase.


Examples of plants with the above-mentioned traits are non-exhaustively listed in Table A.









TABLE A






Effected target or expressed



No.
principle(s)
Crop phenotype/Tolerance to







A-1
Acetolactate synthase (ALS)
Sulfonylureas, Imidazolinones,




Triazolopyrimidines,




Pyrimidyloxybenzoates, Phtalides


A-2
AcetylCoA Carboxylase (ACCase)
Aryloxyphenoxyalkanecarboxylic acids,




cyclohexanediones


A-3
Hydroxyphenylpyruvate
Isoxazoles such as Isoxaflutol or



dioxygenase (HPPD)
Isoxachlortol, Triones such as mesotrione




or sulcotrione


A-4
Phosphinothricin acetyltransferase
Phosphinothricin


A-5
O-Methyl transferase
altered lignin levels


A-6
Glutamine synthetase
Glufosinate, Bialaphos


A-7
Adenylosuccinate Lyase (ADSL)
Inhibitors of IMP and AMP synthesis


A-8
Adenylosuccinate Synthase
Inhibitors of adenylosuccinate synthesis


A-9
Anthranilate Synthase
Inhibitors of tryptophan synthesis and




catabolism


A-10
Nitrilase
3,5-dihalo-4-hydroxy-benzonitriles such as




Bromoxynil and Ioxinyl


A-11
5-Enolpyruvyl-3phosphoshikimate
Glyphosate or sulfosate



Synthase (EPSPS)



A-12
Glyphosate oxidoreductase
Glyphosate or sulfosate


A-13
Protoporphyrinogen oxidase 
Diphenylethers, cyclic imides,



(PROTOX)
phenylpyrazoles, pyridin derivatives,




phenopylate, oxadiazoles, etc.


A-14
Cytochrome P450 eg. P450 SU1
Xenobiotics and herbicides such as




Sulfonylureas


A-15
Dimboa biosynthesis (Bxl gene)
Helminthosporium turcicum,




Rhopalosiphum maydis, Diplodia maydis,




Ostrinia nubilalis, lepidoptera sp.


A-16
CMIII (small basic maize seed 
plant pathogenes eg. fusarium, alternaria,



peptide)
sclerotina


A-17
Corn-SAFP (zeamatin)
plant pathogenes eg. fitsarium, alternaria,




sclerotine, rhizoctonia, chaetomium,




phycomyces


A-18
Hml gene
Cochliobulus


A-19
Chitinases
plant pathogenes


A-20
Glucanases
plant pathogenes


A-21
Coat proteins
viruses such as maize dwarf mosaic virus,




maize chlorotic dwarf virus


A-22
Bacillus thuringiensis toxins, VIP 3, 
lepidoptera, coleoptera, diptera,



Bacillus cereus toxins, Photorabdus
nematodes, eg. ostrinia nubilalis, heliothis



and Xenorhabdus toxins
zea, armyworms eg. Spodoptera




frugipercla, corn rootworms, sesamia




sp., black cutworm, asian corn borer,




weevils


A-23
3-Hydroxysteroid oxidase
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. Spodoptera




frugiperda, corn rootworms, sesamia




sp., black cutworm, asian corn borer,




weevils


A-24
Peroxidase
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. spodoptera frugiperda,




corn rootworms, sesamia sp.,black




cutworm, asian corn borer, weevils


A-25
Aminopeptidase inhibitors eg. 
lepidoptera, coleoptera, diptera,



Leucine aminopeptidase inhibitor 
nematodes, eg. ostrinia nubilalis, heliothis



(LAPI)
zea, armyworms eg. spodoptera




frugiperda, corn rootworms, sesamia sp.,




black cutworm, asian corn borer, weevils


A-26
Limonene synthase
corn rootworms


A-27
Lectines
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. spodoptera frugiperda,




corn rootworms, sesamia sp., black




cutworm, asian corn borer, weevils


A-28
Protease Inhibitors eg. cystatin, 
weevils, corn rootworm



patatin, virgiferin, CPTI



A-29
ribosome inactivating protein
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. spodoptera frugiperda,




corn rootworms, sesamia sp.,black




cutworm, asian corn borer, weevils


A-30
maize 5C9 polypeptide
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. spodoptera frugiperda,




corn rootworms, sesamia sp.,black




cutworm, asian corn borer, weevils


A-31
HMG-CoA reductase
lepidoptera, coleoptera, diptera,




nematodes, eg. ostrinia nubilalis, heliothis




zea, armyworms eg. spodoptera frugiperda,




corn rootworms, sesamia sp.,black




cutworm, asian corn borer, weevils


A-32
Inhibition of protein synthesis
Chloroactanilides such as Alachlor,




Acetochlor, Dimethenamid


A-33
Hormone mimic
2,4-D, Mecoprop-P









Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention show altered quantity, quality and/or storage-stability of the harvested product and/or altered properties of specific ingredients of the harvested product such as:

    1) transgenic plants which synthesize a modified starch, which in its physical-chemical characteristics, in particular the amylose content or the amylose/amylopectin ratio, the degree of branching, the average chain length, the side chain distribution, the viscosity behaviour, the gelling strength, the starch grain size and/or the starch grain morphology, is changed in comparison with the synthesised starch in wild type plant cells or plants, so that this is better suited for special applications.
    2) transgenic plants which synthesize non starch carbohydrate polymers or which synthesize non starch carbohydrate polymers with altered properties in comparison to wild type plants without genetic modification. Examples are plants producing polyfructose, especially of the inulin and levan-type, plants producing alpha 1,4 glucans, plants producing alpha-1,6 branched alpha-1,4-glucans, plants producing alternan,
    3) transgenic plants which produce hyaluronan.


Particularly useful transgenic plants which may be treated according to the invention are plants containing transformation events, or combination of transformation events, that are the subject of petitions for non-regulated status, in the United States of America, to the Animal and Plant Health Inspection Service (APHIS) of the United States Department of Agriculture (USDA) whether such petitions are granted or are still pending. At any time this information is readily available from APHIS (4700 River Road Riverdale, Md. 20737, USA), for instance on its internet site (URL http://www.aphis.usda.gov/brs/not_reg.html). On the filing date of this application the petitions for nonregulated status that were pending with APHIS or granted by APHIS were those listed in table B which contains the following information:













Event
Company
Description
Crop







ASR368
Scotts Seeds
Glyphosate tolerance derived by inserting a
Agrostis stolonifera




modified 5-enolpyruvylshikimate-3-phosphate 
Creeping Bentgrass




synthase (EPSPS) encoding gene from 





Agrobacterium tumefaciens, parent line B99061



H7-1
Monsanto Company
Glyphosate herbicide tolerant sugar beet
Beta vulgaris




produced by inserting a gene encoding the





enzyme 5-enolypyruvylshikimate-3-phosphate





synthase (EPSPS) from the CP4 strain of





Agrobacterium tumefaciens.



T120-7
Bayer CropScience (Aventis 
Introduction of the PPT-acetyltransferase
Beta vulgaris



CropScience(AgrEvo))
(PAT) encoding gene from Streptomyces





viridochromogenes, an aerobic soil bacteria.





PPT normally acts to inhibit glutamine





synthetase, causing a fatal accumulation of





ammonia. Acetylated PPT is inactive.



GTSB77
Novartis Seeds; 
Glyphosate herbicide tolerant sugar beet
Beta vulgaris



Monsanto Company
produced by inserting a gene encoding the
sugar Beet




enzyme 5-enolypyruvylshikimate-3-phosphate





synthase (EPSPS) from the CP4 strain of





Agrobacterium tumefaciens.



23-18-17, 23-198
Monsanto Company
High laurate (12:0) and myristate (14:0)
Brassica napus



(formerly Calgene)
canola produced by inserting a thioesterase
(Argentine Canola)




encoding gene from the California bay laurel





(Umbellularia californica).



45A37, 46A40
Pioneer Hi-Bred
High oleic acid and low linolenic acid canola
Brassica napus



International Inc.
produced through a combination of chemical
(Argentine Canola)




mutagenesis to select for a fatty acid





desaturase mutant with elevated oleic acid,





and traditional back-crossing to introduce the 





low linolenic acid trait.



46A12, 46A16
Pioneer Hi-Bred
Combination of chemical mutagenesis, to
Brassica napus



International Inc.
achieve the high oleic acid trait, and
(Argentine Canola)




traditional breeding with registered canola 





varieties.



GT200
Monsanto Company
Glyphosate herbicide tolerant canola
Brassica napus




produced by inserting genes encoding the
(Argentine Canola)




enzymes 5-enolypyruvylshikimate-3-





phosphate synthase (EPSPS) from the CP4





strain of Agrobacterium tumefaciens and





glyphosateoxidase from Ochrobactrum anthropi.



GT73, RT73
Monsanto Company
Glyphosate herbicide tolerant canola
Brassica napus




produced by inserting genes encoding the
(Argentine Canola)




enzymes 5-enolypyruvylshikimate-3-





phosphate synthase (EPSPS) from the CP4





strain of Agrobacterium tumefaciens and





glyphosate oxidase from Ochrobactrum anthropi.



HCN10
Aventis CropScience
Introduction of the PPT-acetyltransferase
Brassica napus




(PAT) encoding gene from Streptomyces
(Argentine Canola)




viridochromogenes, an aerobic soil bacteria.





PPT normally acts to inhibit glutamine





synthetase, causing a fatal accumulation of 





ammonia. Acetylated PPT is inactive.



HCN92
Bayer CropScience (Aventis 
Introduction of the PPT-acetyltransferase
Brassica napus



Crop Science(AgrEvo))
(PAT) encoding gene from Streptomyces
(Argentine Canola)




viridochromogenes, an aerobic soil bacteria.





PPT normally acts to inhibit glutamine





synthetase, causing a fatal accumulation of 





ammonia. Acetylated PPT is inactive.



MS1, RF1 
Aventis CropScience (formerly
Male-sterility, fertility restoration, pollination
Brassica napus


=>PGS1
Plant Genetic Systems)
control system displaying glufosinate
(Argentine Canola)




herbicide tolerance. MS lines contained the





barnase gene from Bacillus amyloliquefaciens, 





RF lines contained the barstar gene from the 





same bacteria, and both lines contained the 





phosphinothricin N-acetyltransferase (PAT) 





encoding gene from Streptomyces hygroscopicus.



MS1, RF2
Aventis CropScience (formerly
Male-sterility, fertility restoration, pollination
Brassica napus


=>PGS2
Plant Genetic Systems)
control system displaying glufosinate
(Argentine Canola)




herbicide tolerance. MS lines contained the





barnase gene from Bacillus amyloliquefaciens, 





RF lines contained the barstar gene from the 





same bacteria, and both lines contained the 





phosphinothricin N-acetyltransferase (PAT) 





encoding gene from Streptomyces hygroscopicus.



MS8xRF3
Bayer CropScience (Aventis 
Male-sterility, fertility restoration, pollination
Brassica napus



CropScience(AgrEvo))
control system displaying glufosinate
(Argentine Canola)




herbicide tolerance. MS lines contained the





barnase gene from Bacillus amyloliquefaciens, 





RF lines contained the barstar gene from the 





same bacteria, and both lines contained the 





phosphinothricin N-acetyltransferase (PAT) 





encoding gene from Streptomyces hygroscopicus.



NS738, NS1471,
Pioneer Hi-Bred
Selection of somaclonal variants with altered
Brassica napus


NS1473
International Inc.
acetolactate synthase (ALS) enzymes,
(Argentine Canola)




following chemical mutagenesis. Two lines





(P1, P2) were initially selected with 





modifications at different unlinked loci.





NS738 contains the P2 mutation only.



OXY-235
Aventis CropScience (formerly
Tolerance to the herbicides bromoxynil and
Brassica napus



Rhône Poulenc Inc.)
ioxynil by incorporation of the nitrilase gene 
(Argentine Canola)




from Klebsiella pneumoniae.



PHY14, PHY35
Aventis CropScience (formerly
Male sterility was via insertion of the barnase
Brassica napus



Plant Genetic Systems)
ribonuclease gene from Bacillus amyloliquefaciens; 
(Argentine Canola)




fertility restoration by insertion of the barstar 





RNase inhibitor; PPT resistance was via PPT-





acetyltransferase (PAT) from Streptomyces





hygroscopicus.



PHY36
Aventis CropScience (formerly
Male sterility was via insertion of the barnase
Brassica napus



Plant Genetic Systems)
ribonuclease gene from Bacillus
(Argentine Canola)




amyloliquefaciens; fertility restoration by





insertion of the barstar RNase inhibitor; PPT





resistance was via PPT-acetyltransferase





(PAT) from Streptomyces hygroscopicus.



T45 (HCN28)
Bayer CropScience (Aventis
Introduction of the PPT-acetyltransferase
Brassica napus



CropScience(AgrEvo))
(PAT) encoding gene from Streptomyces
(Argentine Canola)




viridochromogenes, an aerobic soil bacteria.





PPT normally acts to inhibit glutamine





synthetase, causing a fatal accumulation of





ammonia. Acetylated PPT is inactive.



HCR-1
Bayer CropScience (Aventis
Introduction of the glufosinate ammonium
Brassica rapa



CropScience(AgrEvo))
herbicide tolerance trait from transgenic B. napus
(Polish Canola)




line T45. This trait is mediated by the





phosphinothricin acetyltransferase (PAT)





encoding gene from S. viridochromogenes.



ZSR500/502
Monsanto Company
Introduction of a modified 5-enol-
Brassica rapa




pyruvylshikimate-3-phosphate synthase
(Polish Canola)




(EPSPS) and a gene from Achromobacter sp





that degrades glyphosate by conversion to





aminomethylphosphonic acid (AMPA) and





glyoxylate by interspecific crossing with GT73.



55-1/63-1
Cornell University
Papaya ringspot virus (PRSV) resistant papaya 
Carica papaya




produced by inserting the coat protein (CP) 
(Papaya)




encoding sequences from this plant potyvirus.



RM3-3, RM3-4,
Bejo Zaden BV
Male sterility was via insertion of the barnase
Cichorium intybus


RM3-6

ribonuclease gene from Bacillus amyloliquefaciens;
(Chicory)




PPT resistance was via the bar gene from 





S. hygroscopicus, which encodes the PAT enzyme.



A, B
Agritope Inc.
Reduced accumulation of S-adenosylmethionine 
Cucumis melo




(SAM), and consequently reduced ethylene 
(Melon)




synthesis, by introduction of the gene encoding 





S-adenosylmethionine hydrolase.



CZW-3
Asgrow (USA); Seminis 
Cucumber mosiac virus (CMV), zucchini
Cucurbita pepo



Vegetable Inc. (Canada)
yellows mosaic (ZYMV) and watermelon
(Squash)




mosaic virus (WMV) 2 resistant squash 





(Curcurbita pepo) produced by inserting the





coat protein (CP) encoding sequences from





each of these plant viruses into the host





genome.



ZW20
Upjohn (USA); Seminis 
Zucchini yellows mosaic (ZYMV) and 
Cucurbita pepo



Vegetable Inc. (Canada)
watermelon mosaic virus (WMV) 2 resistant
(Squash)




squash (Curcurbita pepo) produced by





inserting the coat protein (CP) encoding





sequences from each of these plant





potyviruses into the host genome.



66
Florigene Pty Ltd.
Delayed senescence and sulfonylurea
Dianthus




herbicide tolerant carnations produced by
caryophyllus




inserting a truncated copy of the carnation
(Carnation)




aminocyclopropane cyclase (ACC) synthase





encoding gene in order to suppress expression





of the endogenous unmodified gene, which is





required for normal ethylene biosynthesis.





Tolerance to sulfonyl urea herbicides was via





the introduction of a chlorsulfuron tolerant





version of the acetolactate synthase (ALS)





encoding gene from tobacco.



4, 11, 15, 16
Florigene Pty Ltd.
Modified colour and sulfonylurea herbicide
Dianthus




tolerant carnations produced by inserting two
caryophyllus




anthocyanin genes whose biosynthetic 
(Carnation)




expression in a violet/mauve results





colouration. Tolerance to sulfonylurea





herbicides was via the introduction of a





chlorsulfuron tolerant version of the





acetolactate synthase (ALS) encoding gene





from tobacco.



959A, 988A, 1226A, 
Florigene Pty Ltd.
Introduction of two anthocyanin biosynthetic
Dianthus


1351A, 1363A, 1400A

genes to result in a violet/mauve colouration;
caryophyllus




Introduction of a variant form of acetolactate 
(Carnation)




synthase (ALS).



A2704-12, A2704-21, 
Aventis CropScience
Glufosinate ammonium herbicide tolerant
Glycine max L.


A5547-35

soybean produced by inserting a modified
(Soybean)




phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium





Streptomyces viridochromogenes.



A5547-127
Bayer CropScience (Aventis 
Glufosinate ammonium herbicide tolerant
Glycine max L.



CropScience(AgrEvo))
soybean produced by inserting a modified
(Soybean)




phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium





Streptomyces viridochromogenes.



DP356043
Pioneer Hi-Bred
Soybean event with two herbicide tolerance
Glycine max L.



International Inc.
genes: glyphosate N-acetlytransferase, which
(Soybean)




detoxifies glyphosate, and a modified acetolactate 





synthase (A



G94-1, G94-19,
DuPont Canada Agricultural 
High oleic acid soybean produced by
Glycine max L.


G168
Products
inserting a second copy of the fatty acid
(Soybean)




desaturase (GmFad2-1) encoding gene from





soybean, which resulted in “silencing” of the





endogenous host gene.



GTS 40-3-2
Monsanto Company
Glyphosate tolerant soybean variety produced
Glycine max L.




by inserting a modified 5-enolpyruvylshikimate-
(Soybean)




3-phosphate synthase (EPSPS) encoding gene 





from the soil bacterium Agrobacterium tumefaciens.



GU262
Bayer CropScience (Aventis
Glufosinate ammonium herbicide tolerant
Glycine max L.



CropScience(AgrEvo))
soybean produced by inserting a modified
(Soybean)




phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium





Streptomyces viridochromogenes.



MON89788
Monsanto Company
Glyphosate-tolerant soybean produced by
Glycine max L.




inserting a modified 5-enolpyruvylshikimate-
(Soybean)




3-phosphate synthase (EPSPS) encoding aroA





(epsps) gene from Agrobacterium tumefaciens CP4.



OT96-15
Agriculture & Agri-Food Canada
Low linolenic acid soybean produced through
Glycine max L.




traditional cross-breeding to incorporate the novel 
(Soybean)




trait from a naturally occurring fan1 gene mutant 





that was selected for low linolenic acid.



W62, W98
Bayer CropScience (Aventis
Glufosinate ammonium herbicide tolerant
Glycine max L.



CropScience(AgrEvo))
soybean produced by inserting a modified
(Soybean)




phosphinothricin acetyltransferase (PAT) 





encoding gene from the soil bacterium





Streptomyces hygroscopicus.



15985
Monsanto Company
Insect resistant cotton derived by
Gossypium hirsutum




transformation of the DP50B parent variety,
L. (Cotton)




which contained event 531 (expressing





Cry1Ac protein), with purified plasmid DNA





containing the cry2Ab gene from B. thuringiensis





subsp. kurstaki.



19-51A
DuPont Canada Agricultural
Introduction of a variant form of acetolactate
Gossypium hirsutum



Products
synthase (ALS).
L. (Cotton)


281-24-236
DOW AgroSciences LLC
Insect-resistant cotton produced by inserting
Gossypium hirsutum




the cry1F gene from Bacillus thuringiensis var. 
L. (Cotton)




aizawai. The PAT encodin ggene from 





Streptomyces viridochromogenes was introduced 





as a selectable marker.



3006-210-23
DOW AgroSciences LLC
Insect-resistant cotton produced by inserting
Gossypium hirsutum




the cry1Ac gene from Bacillus thuringiensis
L. (Cotton)




subsp. kurstaki. encoding gene from 





Streptomyces viridochromogenes was 





introduced as a selectable marker.



31807/31808
Calgene Inc.
Insect-resistant and bromoxynil herbicide
Gossypium hirsutum




tolerant cotton produced by inserting the
L. (Cotton)




cry1Ac gene from Bacillus thuringiensis and





a nitrilase encoding gene from Klebsiella





pneumoniae.



BXN
Calgene Inc.
Bromoxynil herbicide tolerant cotton
Gossypium hirsutum




produced by inserting a nitrilase encoding
L. (Cotton)




gene from Klebsiella pneumoniae.



COT102
Syngenta Seeds, Inc.
Insect-resistant cotton produced by inserting
Gossypium hirsutum




the vip3A(a) gene from Bacillus thuringiensis
L. (Cotton)




AB88. The APH4 encoding gene from E. coli





was introduced as a selectable marker.



DAS-21Ø23-5 x
DOW AgroSciences LLC
WideStrike TM, a stacked insect-resistant
Gossypium hirsutum


DAS-24236-5

cotton derived from conventional cross-
L. (Cotton)




breeding of parental lines 3006-210-23





(OECD identifier: DAS-21Ø23-5) and 281-





24-236 (OECD identifier: DAS-24236-5).



DAS-21∅23-5 x
DOW AgroSciences LLC and
Stacked insect-resistant and glyphosate-
Gossypium hirsutum


DAS-24236-5 x
Pioneer Hi-Bred International
tolerant cotton derived from conventional
L. (Cotton)


MON88913
Inc.
cross-breeding of WideStrike cotton (OECD





identifier: DAS-21Ø23-5 x DAS-24236-5)





with MON88913, known as RoundupReady





Flex (OECD identifier: MON-88913-8).



DAS-21Ø23-5 x
DOW AgroSciences LLC
WideStrike TM/Roundup Ready ® cotton, a
Gossypium hirsutum


DAS-24236-5 x

stacked insect-resistant and glyphosate-
L. (Cotton)


MON-Ø1445-2

tolerant cotton derived from conventional





cross-breeding of WideStrike cotton (OECD





identifier: DAS-21Ø23-5 x DAS-24236-5)





with MON1445 (OECD identifier: MON-Ø1445-2).



LLCotton25
Bayer CropScience (Aventis
Glufosinate ammonium herbicide tolerant
Gossypium hirsutum



CropScience(AgrEvo))
cotton produced by inserting a modified
L. (Cotton)




phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium 





Streptomyces hygroscopicus.



LLCotton25 x
Bayer CropScience (Aventis
Stacked herbicide tolerant and insect resistant
Gossypium hirsutum


MON15985
CropScience(AgrEvo))
cotton combining tolerance to glufosinate
L. (Cotton)




ammonium herbicide LLCotton25





(OECD identifier: ACS-GHØØ1-3) with





resistance to insects from MON15985





(OECD identifier: MON-15985-7)



GBH614
Bayer CropScience (Aventis
Glyphosate herbicide tolerant cotton
Gossypium hirsutum



CropScience(AgrEvo))
produced by inserting 2mepsps gene into
L. (Cotton)




variety Coker312 by Agrobacterium under the 





control of Ph4a748At and TPotpC



MON1445/1698
Monsanto Company
Glyphosate herbicide tolerant cotton
Gossypium hirsutum




produced by inserting a naturally glyphosate
L. (Cotton)




tolerant form of the enzyme 5-enolpyruvylshikimate-





3-phosphate synthase (EPSPS) from A. tumefaciens





strain CP4.



MON15985 x
Monsanto Company
Stacked insect resistant and glyphosate
Gossypium hirsutum


MON88913

tolerant cotton produced by conventional
L. (Cotton)




cross-breeding of the parental lines





MON88913 (OECD identifier: MON-88913-8) 





and 15985 (OECD identifier: MON-15985-7). 





Glyphosate tolerance is derived from 





MON88913 which contains two genes encoding 





the enzyme 5-enolypyruvylshikimate-3-phosphate 





synthase (EPSPS) from the CP4 strain of 





Agrobacterium tumefaciens. Insect resistance is 





derived MON15985 which was produced by 





transformation of the DP50B parent variety, which 





contained event 531(expressing Cry1Ac protein), 





with purified plasmid DNA containing the cry2Ab 





gene from B. thuringiensis subsp. kurstaki.



MON-15985-7 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Gossypium hirsutum


MON-Ø1445-2

cotton derived from conventional cross-
L. (Cotton)




breeding of the parental lines 15985 (OECD





identifier: MON-15985-7) and MON1445 





(OECD identifier: MON-Ø1445-2).



MON531/757/1076
Monsanto Company
Insect-resistant cotton produced by inserting
Gossypium hirsutum




the cry1Ac gene from Bacillus thuringiensis
L. (Cotton)




subsp. kurstaki HD-73 (B.t.k.).



MON88913
Monsanto Company
Glyphosate herbicide tolerant cotton
Gossypium hirsutum




produced by inserting two genes encoding the
L. (Cotton)




enzyme 5-enolypyruvylshikimate-3-phosphate





synthase (EPSPS) from the CP4 strain of





Agrobacterium tumefaciens.



MON-ØØ531-6 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Gossypium hirsutum


MON-Ø1445-2

cotton derived from conventional cross-
L. (Cotton)




breeding of the parental lines MON531





(OECD identifier: MON-ØØ531-6) and





MON1445 (OECD identifier: MON-Ø1445-2).



X81359
BASF Inc.
Tolerance to imidazolinone herbicides by
Helianthus




selection of a naturally occurring mutant. 
annuus (Sunflower)


RH44
BASF Inc.
Selection for a mutagenized version of the
Lens culinaris




enzyme acetohydroxyacid synthase (AHAS),
(Lentil)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



FP967
University of Saskatchewan, 
A variant form of acetolactate synthase (ALS)
Linum usitatissimum



Crop Dev. Centre
was obtained from a chlorsulfuron tolerant 
L. (Flax, Linseed)




line of A. thaliana and used to transform flax. 



5345
Monsanto Company
Resistance to lepidopteran pests through the
Lycopersicon




introduction of the cry1Ac gene from Bacillus
esculentum (Tomato)




thuringiensis subsp. Kurstaki.



8338
Monsanto Company
Introduction of a gene sequence encoding the
Lycopersicon




enzyme 1-amino-cyclopropane-1-carboxylic
esculentum (Tomato)




acid deaminase (ACCd) that metabolizes the





precursor of the fruit ripening hormone





ethylene.



1345-4
DNA Plant Technology 
Delayed ripening tomatoes produced by
Lycopersicon



Corporation
inserting an additional copy of a truncated
esculentum (Tomato)




gene encoding 1-aminocyclopropane-1-





carboxyllic acid (ACC) synthase, which





resulted in downregulation of the endogenous





ACC synthase and reduced ethylene 





accumulation.



35 1N
Agritope Inc.
Introduction of a gene sequence encoding the
Lycopersicon




enzyme S-adenosylmethionine hydrolase that
esculentum (Tomato)




metabolizes the precursor of the fruit ripening 





hormone ethylene



B, Da, F
Zeneca Seeds
Delayed softening tomatoes produced by
Lycopersicon




inserting a truncated version of the
esculentum (Tomato)




polygalacturonase (PG) encoding gene in the





sense or anti-sense orientation in order to





reduce expression of the endogenous PG





gene, and thus reduce pectin degradation.



FLAVR SAVR
Calgene Inc.
Delayed softening tomatoes produced by
Lycopersicon




inserting an additional copy of the
esculentum (Tomato)




polygalacturonase (PG) encoding gene in the





anti-sense orientation in order to reduce





expression of the endogenous PG gene and





thus reduce pectin degradation.



J101, J163
Monsanto Company and 
Glyphosate herbicide tolerant alfalfa (lucerne) 
Medicago sativa



Forage Genetics International
produced by inserting a gene encoding 
(Alfalfa)




the enzyme 5-enolypyruvylshikimate-3-phosphate 





synthase (EPSPS) from the CP4 strain of





Agrobacterium tumefaciens.



C/F/93/08-02
Societe National d'Exploitation 
Tolerance to the herbicides bromoxynil and
Nicotiana tabacun



des Tabacs et Allumettes
ioxynil by incorporation of the nitrilase gene 
L. (Tobacco)




from Klebsiella pneumoniae.



Vector 21-41
Vector Tobacco Inc.
Reduced nicotine content through
Nicotiana tabacun




introduction of a second copy of the tobacco
L. (Tobacco)




quinolinic acid phosphoribosyltransferase





(QTPase) in the antisense orientation. The





NPTII encoding gene from E. coli was





introduced as a selectable marker to identify





transformants.



CL121, CL141,
BASF Inc.
Tolerance to the imidazolinone herbicide,
Oryza sativa (Rice)


CFX51

imazethapyr, induced by chemical





mutagenesis of the acetolactate synthase





(ALS) enzyme using ethyl methanesulfonate





(EMS).



IMINTA-1,
BASF Inc.
Tolerance to imidazolinone herbicides
Oryza sativa (Rice)


IMINTA-4

induced by chemical mutagenesis of the 





acetolactate synthase (ALS) enzyme using





sodium azide.



LLRICE06,
Aventis CropScience
Glufosinate ammonium herbicide tolerant rice
Oryza sativa (Rice)


LLRICE62

produced by inserting a modified





phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium





Streptomyces hygroscopicus).



LLRICE601
Bayer CropScience (Aventis 
Glufosinate ammonium herbicide tolerant rice
Oryza sativa (Rice)



CropScience(AgrEvo))
produced by inserting a modified





phosphinothricin acetyltransferase (PAT)





encoding gene from the soil bacterium





Streptomyces hygroscopicus).



C5
United States Department of 
Plum pox virus (PPV) resistant plum tree
Prunus domestica



Agriculture-Agricultural 
produced through Agrobacterium-mediated
(Plum)



Research Service
transformation with a coat protein (CP) gene





from the virus.



PWC16
BASF Inc.
Tolerance to the imidazolinone herbicide,
Oryza sativa (Rice)




imazethapyr, induced by chemical mutagenesis





of the acetolactate synthase (ALS) enzyme using 





ethyl methanesulfonate (EMS).



ATBT04-6,
Monsanto Company
Colorado potato beetle resistant potatoes
Solanum tuberosum


ATBT04-27,

produced by inserting the cry3A gene from
L. (Potato)


ATBT04-30,

Bacillus thuringiensis (subsp. Tenebrionis).



ATBT04-31,





ATBT04-36,





SPBT02-5,





SPBT02-7





BT6, BT10, BT12,
Monsanto Company
Colorado potato beetle resistant potatoes 
Solanum tuberosum


BT16, BT17,

produced by inserting the cry3A gene from
L. (Potato)


BT18, BT23

Bacillus thuringiensis (subsp. Tenebrionis).



RBMT15-101,
Monsanto Company
Colorado potato beetle and potato virus Y (PVY)
Solanum tuberosum


SEMT15-02,

resistant potatoes produced by inserting the 
L. (Potato)


SEMT15-15

cry3A gene from Bacillus thuringiensis





(subsp. Tenebrionis) and the coat protein 





encoding gene from PVY.



RBMT21-129,
Monsanto Company
Colorado potato beetle and potato leafroll
Solanum tuberosum


RBMT21-350,

virus (PLRV) resistant potatoes produced by
L. (Potato)


RBMT22-082

inserting the cry3A gene from Bacillus





thuringiensis (subsp. Tenebrionis) and the 





replicase encoding gene from PLRV.



AP205CL
BASF Inc.
Selection for a mutagenized version of the
Triticum aestivum




enzyme acetohydroxyacid synthase (AHAS),
(Wheat)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



AP602CL
BASF Inc.
Selection for a mutagenized version of the
Triticum aestivum




enzyme acetohydroxyacid synthase (AHAS),
(Wheat)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



BW255-2, 
BASF Inc.
Selection for a mutagenized version of the
Triticum aestivum


BW238-3

enzyme acetohydroxyacid synthase (AHAS),
(Wheat)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



BW7
BASF Inc.
Tolerance to imidazolinone herbicides
Triticum aestivum




induced by chemical mutagenesis of the
(Wheat)




acetohydroxyacid synthase (AHAS) gene





using sodium azide.



MON71800
Monsanto Company 
Glyphosate tolerant wheat variety produced
Triticum aestivum




by inserting a modified 5-enolpyruvylshikimate-
(Wheat)




3-phosphate synthase (EPSPS) encoding gene 





from the soil bacterium Agrobacterium





tumefaciens, strain CP4.



SWP965001
Cyanamid Crop Protection
Selection for a mutagenized version of the
Triticum aestivum




enzyme acetohydroxyacid synthase (AHAS),
(Wheat)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



Teal 11A
BASF Inc.
Selection for a mutagenized version of the
Triticum aestivum




enzyme acetohydroxyacid synthase (AHAS),
(Wheat)




also known as acetolactate synthase (ALS) or





acetolactate pyruvate-lyase.



176
Syngenta Seeds, Inc.
Insect-resistant maize produced by inserting
Zea mays L. (Maize)




the cry1Ab gene from Bacillus thuringiensis





subsp. kurstaki. The genetic modification





affords resistance to attack by the European





corn borer (ECB).



3751IR
Pioneer Hi-Bred 
Selection of somaclonal variants by culture of
Zea mays L. (Maize)



International Inc.
embryos on imidazolinone containing media.



676, 678, 680
Pioneer Hi-Bred
Male-sterile and glufosinate ammonium
Zea mays L. (Maize)



International Inc.
herbicide tolerant maize produced by





inserting genes encoding DNA adenine





methylase and phosphinothricin





acetyltransferase (PAT) from Escherichia coli





and Streptomyces viridochromogenes, respectively.



ACS-ZMØØ3-2 x
Bayer CropScience (Aventis 
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ81Ø-6
CropScience(AgrEvo))
corn hybrid derived from conventional cross-





breeding of the parental lines T25 (OECD





identifier: ACS-ZMØØ3-2) and MON810





(OECD identifier:MON-ØØ810-6).



B16 (DLL25)
Dekalb Genetics Corporation
Glufosinate ammonium herbicide tolerant
Zea mays L. (Maize)




maize produced by inserting the gene





encoding phosphinothricin acetyltransferase





(PAT) from Streptomyces hygroscopicus.



BT11 (X4334CBR,
Syngenta Seeds, Inc.
Insect-resistant and herbicide tolerant maize
Zea mays L. (Maize)


X4734CBR)

produced by inserting the cry1Ab gene from





Bacillus thuringiensis subsp. kurstaki, and the





phosphinothricin N-acetyltransferase (PAT)





encoding gene from S. viridochromogenes.



BT11 x MIR604
Syngenta Seeds, Inc.
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)




maize produced by conventional cross





breeding of parental lines BT11 (OECD





unique identifier: SYN-BTØ11-1) and





MIR604 (OECD unique identifier: SYN-





IR6Ø5-5). Resistance to the European Corn





Borer and tolerance to the herbicide





glufosinate ammonium (Liberty) is derived





from BT11, which contains the cry1Ab gene





from Bacillus thuringiensis subsp. kurstaki,





and the phosphinothricin N-acetyltransferase





(PAT) encoding gene from S. viridochromogenes. 





Corn rootworm-resistance is derived from 





MIR604 which contains the mcry3A gene from 





Bacillus thuringiensis.



BT11 x MIR604 x
Syngenta Seeds, Inc. 
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


GA21

maize produced by conventional cross





breeding of parental lines BT11 (OECD





unique identifier: SYN-BTØ11-1), MIR604





(OECD unique identifier: SYN-IR6Ø5-5) and 





GA21 (OECD unique identifier: MON-





ØØØ21-9). Resistance to the European Corn





Borer and tolerance to the herbicide





glufosinate ammonium (Liberty) is derived





from BT11, which contains the cry1Ab gene





from Bacillus thuringiensis subsp. kurstaki,





and the phosphinothricin N-acetyltransferase





(PAT) encoding gene from S. viridochromogenes. 





Corn rootworm-resistance is derived from 





MIR604 which contains the mcry3A gene 





from Bacillus thuringiensis. Tolerance to 





glyphosate herbcicide is derived from 





GA21 which contains a a modified EPSPS





gene from maize.



CBH-351
Aventis CropScience
Insect-resistant and glufosinate ammonium
Zea mays L. (Maize)




herbicide tolerant maize developed by





inserting genes encoding Cry9C protein from





Bacillus thuringiensis subsp tolworthi and





phosphinothricin acetyltransferase (PAT)





from Streptomyces hygroscopicus.



DAS-06275-8
DOW AgroSciences LLC
Lepidopteran insect resistant and glufosinate
Zea mays L. (Maize)




ammonium herbicide-tolerant maize variety





produced by inserting the cry1F gene from





Bacillus thuringiensis var aizawai and the





phosphinothricin acetyltransferase (PAT)





from Streptomyces hygroscopicus.



DAS-59122-7
DOW AgroSciences LLC and 
Corn rootworm-resistant maize produced by
Zea mays L. (Maize)



Pioneer Hi-Bred International 
inserting the cry34Ab1 and cry35Ab1 genes




Inc.
from Bacillus thuringiensis strain PS149B1.





The PAT encoding gene from Streptomyces





viridochromogenes was introduced as a





selectable marker.



DAS-59122-7 x 
DOW AgroSciences LLC and
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


NK603
Pioneer Hi-Bred International
maize produced by conventional cross




Inc.
breeding of parental lines DAS-59122-7





(OECD unique identifier: DAS-59122-7) with





NK603 (OECD unique identifier: MON-ØØ6Ø3-6).





Corn rootworm-resistance is derived from 





DAS-59122-7 which contains the cry34Ab1





and cry35Ab1 genes from Bacillus thuringiensis





strain PS149B1. Tolerance to glyphosate 





herbcicide is derived from NK603.



DAS-59122-7 x
DOW AgroSciences LLC and
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


TC1507 x NK603
Pioneer Hi-Bred International
maize produced by conventional cross




Inc.
breeding of parental lines DAS-59122-7





(OECD unique identifier: DAS-59122-7) and





TC1507 (OECD unique identifier: DAS-





Ø15Ø7-1) with NK603 (OECD unique





identifier: MON-ØØ6Ø3-6). Corn rootworm-





resistance is derived from DAS-59122-7





which contains the cry34Ab1and cry35Ab1





genes from Bacillus thuringiensis strain





PS149B1. Lepidopteran resistance and





toleraance to glufosinate ammonium





herbicide is derived from TC1507. Tolerance





to glyphosate herbcicide is derived from





NK603.



DAS-Ø15Ø7-1 x
DOW AgroSciences LLC
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ6Ø3-6

corn hybrid derived from conventional cross-





breeding of the parental lines 1507 (OECD





identifier: DAS-Ø15Ø7-1) and NK603





(OECD identifier: MON-ØØ6Ø3-6).



DBT418
Dekalb Genetics Corporation
Insect-resistant and glufosinate ammonium
Zea mays L. (Maize)




herbicide tolerant maize developed by





inserting genes encoding Cry1AC protein





from Bacillus thuringiensis subsp kurstaki





and phosphinothricin acetyltransferase (PAT)





from Streptomyces hygroscopicus



DK404SR
BASF Inc.
Somaclonal variants with a modified acetyl-
Zea mays L. (Maize)




CoA-carboxylase (ACCase) were selected by





culture of embryos on sethoxydim enriched





medium.



Event 3272
Syngenta Seeds, Inc.
Maize line expressing a heat stable alpha-
Zea mays L. (Maize)




amylase gene amy797E for use in the dry-





grind ethanol process. The phosphomannose





isomerase gene from E.coli was used as a





selectable marker.



EXP1910IT
Syngenta Seeds, Inc. 
Tolerance to the imidazolinone herbicide,
Zea mays L. (Maize)



(formerly Zeneca Seeds)
imazethapyr, induced by chemical





mutagenesis of the acetolactate synthase





(ALS) enzyme using ethyl methanesulfonate





(EMS).



GA21
Monsanto Company
Introduction, by particle bombardment, of a
Zea mays L. (Maize)




modified 5-enolpyruvyl shikimate-3-





phosphate synthase (EPSPS), an enzyme





involved in the shikimate biochemical





pathway for the production of the aromatic





amino acids.



IT
Pioneer Hi-Bred
Tolerance to the imidazolinone herbicide,
Zea mays L. (Maize)



International Inc.
imazethapyr, was obtained by in vitro 





selection of somaclonal variants.



LY038
Monsanto Company
Altered amino acid composition, specifically





elevated levels of lysine, through the





introduction of the cordapA gene, derived





from Corynebacterium glutamicum, encoding





the enzyme dihydrodipicolinate synthase





(cDHDPS).



MIR604
Syngenta Seeds, Inc.
Corn rootworm resistant maize produced by
Zea mays L. (Maize)




transformation with a modified cry3A gene.





The phosphomannose isomerase gene from





E.coli was used as a selectable marker.



MIR604 x GA21
Syngenta Seeds, Inc.
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)




maize produced by conventional cross





breeding of parental lines MIR604 (OECD





unique identifier: SYN-IR6Ø5-5) and GA21





(OECD unique identifier: MON-ØØØ21-9).





Corn rootworm-resistance is derived from





MIR604 which contains the mcry3A gene





from Bacillus thuringiensis. Tolerance to





glyphosate herbcicide is derived from GA21.



MON80100
Monsanto Company
Insect-resistant maize produced by inserting
Zea mays L. (Maize)




the cry1Ab gene from Bacillus thuringiensis





subsp. kurstaki. The genetic modification





affords resistance to attack by the European





corn borer (ECB).



MON802
Monsanto Company
Insect-resistant and glyphosate herbicide
Zea mays L. (Maize)




tolerant maize produced by inserting the





genes encoding the Cry1Ab protein from





Bacillus thuringiensis and the





enolpyruvylshikimate-3-phosphate synthase 5-





(EPSPS) from A. tumefaciens strain CP4.



MON809
Pioneer Hi-Bred 
Resistance to European corn borer (Ostrinia
Zea mays L. (Maize)



Inc. International 
nubilalis) by introduction of a synthetic





cry1Ab gene. Glyphosate resistance via





introduction of the bacterial version of a plant





enzyme, 5-enolpyruvyl shikimate-3-phosphate





synthase (EPSPS).



MON810
Monsanto Company
Insect-resistant maize produced by inserting a
Zea mays L. (Maize)




truncated form of the cry1Ab gene from





Bacillus thuringiensis subsp. kurstaki HD-1.





The genetic modification affords resistance to





attack by the European corn borer (ECB).



MON810 x
Monsanto Company
Stacked insect resistant and glyphosate
Zea mays L. (Maize)


MON88017

tolerant maize derived from conventional





cross-breeding of the parental lines MON810





(OECD identifier: MON-ØØ81Ø-6) and





MON88017 (OECD identifier:MON-88Ø17-3). 





European corn borer (ECB) resistance is





derived from a truncated form of the cry1Ab





gene from Bacillus thuringiensis subsp.





kurstaki HD-1 present in MON810. Corn





rootworm resistance is derived from the





cry3Bb1 gene from Bacillus thuringiensis





subspecies kumamotoensis strain EG4691 





present in MON88017. Glyphosate tolerance 





is derived from a 5-enolpyruvylshikimate-3-





phosphate synthase (EPSPS) encoding gene





from Agrobacterium tumefaciens strain CP4





present in MON88017.



MON832
Monsanto Company
Introduction, by particle bombardment, of
Zea mays L. (Maize)




glyphosate oxidase (GOX) and a modified 5-





enolpyruvyl shikimate-3-phosphate synthase





(EPSPS), an enzyme involved in the





shikimate biochemical pathway for the





production of the aromatic amino acids.



MON863
Monsanto Company
Corn root worm resistant maize produced by
Zea mays L. (Maize)




inserting the cry3Bb1 gene from Bacillus





thuringiensis subsp. kumamotoensis.



MON88017
Monsanto Company
Corn rootworm-resistant maize produced by
Zea mays L. (Maize)




inserting the cry3Bb1 gene from Bacillus





thuringiensis subspecies kumamotoensis





strain EG4691. Glyphosate tolerance derived





by inserting a 5-enolpyruvylshikimate-3-





phosphate synthase (EPSPS) encoding gene





from Agrobacterium tumefaciens strain CP4.



MON89034
Monsanto Company
Maize event expressing two different
Zea mays L. (Maize)




insecticidal proteins from Bacillus





thuringiensis providing resistance to number





of lepidopteran pests.



MON89034 x
Monsanto Company
Stacked insect resistant and glyphosate
Zea mays L. (Maize)


MON88017

tolerant maize derived from conventional





cross-breeding of the parental lines





MON89034 (OECD identifier: MON-89Ø34-3) 





and MON88017 (OECD identifier:MON-





88Ø17-3). Resistance to Lepiopteran insects





is derived from two crygenes present in





MON89043. Corn rootworm resistance is





derived from a single cry genes and





glyphosate tolerance is derived from the 5-





enolpyruvylshikimate-3-phosphate synthase





(EPSPS) encoding gene from Agrobacterium





tumefaciens present in MON88017.



MON-ØØ6Ø3-6 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ81Ø-6

corn hybrid derived from conventional cross-





breeding of the parental lines NK603 (OECD





identifier: MON-ØØ6Ø3-6) and MON810





(OECD identifier: MON-ØØ81Ø-6).



MON-ØØ81Ø-6 x
Monsanto Company
Stacked insect resistant and enhanced lysine
Zea mays L. (Maize)


LY038

content maize derived from conventional





cross-breeding of the parental lines MON810





(OECD identifier: MON-ØØ81Ø-6) and





LY038 (OECD identifier: REN-ØØØ38-3).



MON-ØØ863-5 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ6Ø3-6

corn hybrid derived from conventional cross-





breeding of the parental lines MON863





(OECD identifier:MON-ØØ863-5) and





NK603 (OECD identifier: MON-ØØ6Ø3-6).



MON-ØØ863-5 x
Monsanto Company
Stacked insect resistant corn hybrid derived
Zea mays L. (Maize)


MON-ØØ81Ø-6

from conventional cross-breeding of the





parental lines MON863 (OECD identifier:





MON-ØØ863-5) and MON810 (OECD





identifier: MON-ØØ81Ø-6)



MON-ØØ863-5 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ81Ø-6 x

corn hybrid derived from conventional cross-



MON-ØØ6Ø3-6

breeding of the stacked hybrid MON-∅∅863-





5 x MON-ØØ81Ø-6 and NK603 (OECD





identifier: MON-ØØ6Ø3-6).



MON-ØØØ21-9 x
Monsanto Company
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØ81Ø6

corn hybrid derived from conventional cross-





breeding of the parental lines GA21 (OECD





identifider: MON-ØØØ21-9) and MON810





(OECD identifier: MON-ØØ81Ø-6).



MS3
Bayer CropScience (Aventis
Male sterility caused by expression of the
Zea mays L. (Maize)



CropScience(AgrEvo))
barnase ribonuclease gene from Bacillus





amyloliquefaciens; PPT resistance was via





PPT-acetyltransferase (PAT).



MS6
Bayer CropScience (Aventis
Male sterility caused by expression of the
Zea mays L. (Maize)



CropScience(AgrEvo))
barnase ribonuclease gene from Bacillus





amyloliquefaciens; PPT resistance was via





PPT-acetyltransferase (PAT).



NK603
Monsanto Company
Introduction, by particle bombardment, of a
Zea mays L. (Maize)




modified 5-enolpyruvyl shikimate-3-





phosphate synthase (EPSPS), an enzyme





involved in the shikimate biochemical





pathway for the production of the aromatic





amino acids.



SYN-BTØ11-1 x
Syngenta Seeds, Inc.
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


MON-ØØØ21-9

maize produced by conventional cross





breeding of parental lines BT11 (OECD





unique identifier: SYN-BTØ11-1) and GA21





(OECD unique identifier: MON-ØØØ21-9).



T14, T25
Bayer CropScience (Aventis
Glufosinate herbicide tolerant maize
Zea mays L. (Maize)



CropScience(AgrEvo))
produced by inserting the phosphinothricin





N-acetyltransferase (PAT) encoding gene





from the aerobic actinomycete Streptomyces





viridochromogenes.



TC1507
Mycogen (c/o Dow 
Insect-resistant and glufosinate ammonium
Zea mays L. (Maize)



AgroSciences); Pioneer
herbicide tolerant maize produced by




(c/o Dupont)
inserting the cry1F gene from Bacillus





thuringiensis var. aizawai and the





phosphinothricin N-acetyltransferase encoding 





gene from Streptomyces viridochromogenes.



TC1507 x 
DOW AgroSciences LLC and
Stacked insect resistant and herbicide tolerant
Zea mays L. (Maize)


DAS-59122-7
Pioneer Hi-Bred International
maize produced by conventional cross




Inc.
breeding of parental lines TC1507 (OECD





unique identifier: DAS-Ø15Ø7-1) with DAS-





59122-7 (OECD unique identifier: DAS-





59122-7). Resistance to lepidopteran insects





is derived from TC1507 due the presence of





the cry1F gene from Bacillus thuringiensis





var. aizawai. Corn rootworm-resistance is





derived from DAS-59122-7 which contains





the cry34Ab1 and cry35Ab1genes from





Bacillus thuringiensis strain PS149B1.





Tolerance to glufosinate ammonium





herbcicide is derived from TC1507 from the





phosphinothricin N-acetyltransferase encoding 





gene from Streptomyces viridochromogenes.



DP-∅9814∅-6
Pioneer Hi-Bred
Corn line 98140 was genetically engineered
Zea mays L. (Maize)


(Event 98140)
International Inc.
to express the GAT4621 (glyphosate





acetyltransferase) and ZM-HRA (modified





version of a maize acetolactate synthase)





proteins. The GAT4621 protein, encoded by





the gat4621 gene, confers tolerance to





glyphosate-containing herbicides by





acetylating glyphosate and thereby rendering





it non-phytotoxic. The ZM-HRA protein,





encoded by the zm-hra gene, confers





tolerance to the ALS-inhibiting class of





herbiceides









In a very particular embodiment a method for curatively or preventively controlling the phytopathogenic fungi and/or microorganisms and/or pests of plants or crops is described comprising the use of the combination of ((A) and (B)) or ((A) and (B) and (C)) by application to the seed, plant propagation material, the plant or to the fruit of genetically modified plants wherein the active principle expressed by the genetically modified plant corresponds to a line of table A or B.
At certain application rates, the active compound combinations according to the invention may also have a strengthening effect in plants. Accordingly, they are also suitable for mobilizing the defense system of the plant against attack by unwanted phytopathogenic fungi and/or microorganisms and/or viruses. This may, if appropriate, be one of the reasons of the enhanced activity of the combinations according to the invention, for example against fungi. Plant-strengthening (resistance-inducing) substances are to be understood as meaning, in the present context, those substances or combinations of substances which are capable of stimulating the defense system of plants in such a way that, when subsequently inoculated with unwanted phytopathogenic fungi and/or microorganisms and/or pests and/or viruses, the treated plants display a substantial degree of resistance to these phytopathogenic fungi and/or microorganisms and/or pests and/or viruses, Thus, the substances according to the invention can be employed for protecting plants against attack by the abovementioned pathogens within a certain period of time after the treatment. The period of time within which protection is effected generally extends from 1 to 10 days, preferably 1 to 7 days, after the treatment of the plants with the active compounds.
In a further aspect there is provided a composition comprising a combination according to this invention. Preferably the fungicidal and/or insecticidal composition comprises agriculturally acceptable additives, solvents, carriers, surfactants, or extenders.
According to the invention, the term “carrier” denotes a natural or synthetic, organic or inorganic compound with which the active compound A of formula (I) and compound B are combined or associated to make it easier to apply, notably to the parts of the plant. This support is thus preferably inert and should be at least agriculturally acceptable. The support may be a solid or a liquid.
Suitable solid carriers are the following:
e.g. ammonium salts and natural rock powders, such as kaolins, clays, talcum, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth and synthetic rock powders such as highly disperse silica, aluminium oxide and silicates, oil waxes, solid fertilizers, water, alcohols, preferably butanol, organic solvents, mineral and vegetable oils and derivatives thereof;

suitable solid carriers for granules are: for example crushed and fractionated natural rocks such as calcite, marble, pumice, sepiolite, dolomite and synthetic granules of inorganic and organic powders and granules of organic materials such as paper, sawdust, coconut shells, corn stalks and tobacco stalks;

By liquefied gaseous diluents or supports are meant such liquids that are gaseous at normal temperature and under normal pressure, for example, aerosol propellants such as halohydrocarbons as well as butane, propane, nitrogen and carbon dioxide.
It is possible to use in the formulations adhesives such as carboxymethylcellulose, natural and synthetic powdered, granular or latex-like polymers such as gum arabic, polyvinyl alcohol, polyvinyl acetate and natural phospholipids, such as cephalins and lecithins and synthetic phospholipids. Further additives can be mineral or vegetable oils and waxes, optionally modified.
Suitable extenders are, for example, water, polar and non-polar organic chemical liquids, for example from the classes of the aromatic and non-aromatic hydrocarbons (such as paraffins, alkylbenzenes, alkylnaphthalenes, chlorobenzenes), the alcohols and polyols (which, if appropriate, may also be substituted, etherified and/or esterified), the ketones (such as acetone, cyclohexanone), esters (including fats and oils) and (poly)ethers, the unsubstituted and substituted amines, amides, lactams (such as N-alkylpyrrolidones) and lactones, the sulphones and sulphoxides (such as dimethyl sulphoxide).
If the extender used is water, it is also possible to employ, for example, organic solvents as auxiliary solvents. Essentially, suitable liquid solvents are: aromatics such as xylene, toluene or alkyl-naphthalenes, chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons such as cyclohexane or paraffins, for example petroleum fractions, mineral and vegetable oils, alcohols such as butanol or glycol and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethyl sulphoxide, and also water.
The composition according to the invention may also comprise additional components. In particular, the composition may further comprise a surfactant. The surfactant can be an emulsifier, a dispersing agent or a wetting agent of ionic or non-ionic type or a mixture of such surfactants. Mention may be made, for example, of polyacrylic acid salts, lignosulphonic acid salts, phenolsulphonic or naphthalenesulphonic acid salts, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, substituted phenols (in particular alkylphenols or arylphenols), salts of sulphosuccinic acid esters, taurine derivatives (in particular alkyl taurates), phosphoric esters of polyoxyethylated alcohols or phenols, fatty acid esters of polyols, and derivatives of the present compounds containing sulphate, sulphonate and phosphate functions, for example alkylaryl polyglycol-ethers, alkyl sulphonates, alkyl sulphates, aryl sulphonates, protein hydrolyzates, lignosulphite waste liquors and methyl cellulose. The presence of at least one surfactant is generally essential when the active compound and/or the inert support are water-insoluble and when the vector agent for the application is water. Preferably, surfactant content may be comprised from 5% to 40% by weight of the composition.
Suitable emulsifiers and/or foam-forming agents are: for example non-ionic and anionic emulsifiers, such as polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers, suitable dispersants are non-ionic and/or ionic substances, for example from the classes comprising alcohol POE and/or POP ethers, acid and/or POP or POE esters, alkyl-aryl and/or POP or POE ethers, fatty and/or POP—POE adducts, POE and/or POP polyol derivatives, POE and/or POP/sorbitan or sugar adducts, alkyl or aryl sulphates, sulphonates and phosphates or the corresponding PO ether adducts. Furthermore, suitable oligomers or polymers, for example based on vinyl monomers, acrylic acid, EO and/or PO alone or in combination with for example (poly-) alcohols or (poly-amines. Use can also be made of lignin and sulphonic acid derivatives thereof, simple and modified celluloses, aromatic and/or aliphatic sulphonic acids and adducts thereof with formaldehyde. Suitable as dispersants are for example lignosulphite waste liquors and methylcellulose.
Coloring agents such as inorganic pigments, for example iron oxide, titanium oxide, ferrocyanblue, and organic pigments such as alizarin, azo and metallophthalocyanine dyes, and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts can be used.
Optionally, other additional components may also be included, e.g. protective colloids, adhesives, thickeners, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active compounds can be combined with any solid or liquid additive, which complies with the usual formulation techniques.
In general, the composition according to the invention may contain from 0.05 to 99% by weight of active compounds, preferably from 1 to 70% by weight, most preferably from 10 to 50% by weight.
The combination or composition according to the invention can be used as such, in form of their formulations or as the use forms prepared there from, such as aerosol dispenser, capsule suspension, cold fogging concentrate, hot fogging concentrate, encapsulated granule, fine granule, flowable concentrate for seed treatment, ready-to-use solutions, dustable powder, emulsifiable concentrate, emulsion oil in water, emulsion water in oil, macrogranule, microgranule, oil dispersible powder, oil miscible flowable concentrate, oil miscible liquid, froths, paste, seed coated with a pesticide, suspension concentrate (flowable concentrate), suspensions-emulsions-concentrates, soluble concentrate, suspensions, soluble powder, granule, water soluble granules or tablets, water soluble powder for seed treatment, wettable powder, natural and synthetic materials impregnated with active compound, micro-encapsulation in polymeric materials and in jackets for seed, as well as ULV-cold and hot fogging formulations, gas (under pressure), gas generating product, plant rodlet, powder for dry seed treatment, solution for seed treatment, ultra low volume (ULV) liquid, ultra low volume (ULV) suspension, water dispersible granules or tablets, water dispersible powder for slurry treatment.
These formulations are prepared in a known manner by mixing the active compounds or active compound combinations with customary additives, such as, for example, customary extenders and also solvents or diluents, emulsifiers, dispersants, and/or bonding or fixing agent, wetting agents, water repellents, if appropriate siccatives and UV stabilisers, colorants, pigments, defoamers, preservatives, secondary thickeners, adhesives, gibberellins and water as well further processing auxiliaries.
These compositions include not only compositions which are ready to be applied to the plant or seed to be treated by means of a suitable device, such as a spraying or dusting device, but also concentrated commercial compositions which must be diluted before application to the crop.
The control of phytopathogenic fungi and/or microorganisms and/or pests which damage plants post-emergence is carried out primarily by treating the soil and the above-ground parts of plants with crop protection agents. Owing to the concerns regarding a possible impact of crop protection agents on the environment and the health of humans and animals, there are efforts to reduce the amount of active compounds applied.
The active compound combinations according to the invention can be used in its commercially available formulations and in the use forms, prepared from these formulations, as a mixture with other active compounds, such as insecticides, attractants, sterilizing agents, bactericides, acaricides, nematicides, fungicides, growth-regulating substances, herbicides, safeners, fertilizers or semiochemicals.
The treatment of plants and plant parts with the active compound combination according to the invention is carried out directly or by action on their environment, habitat or storage area by means of the normal treatment methods, for example by watering (drenching), drip irrigation, spraying, vaporizing, atomizing, broadcasting, dusting, foaming, spreading-on, painting, injection and as a powder for dry seed treatment, a solution for seed treatment, a water-soluble powder for seed treatment, a water-soluble powder for slurry treatment, or by encrusting, in the case of propagation material, in particular in the case of seeds, furthermore by dry treatments, slurry treatments, liquid treatments, by one- or multi-layer coating. It is furthermore possible to apply the active compounds by the ultra-low volume method, or to inject the active compound preparation or the active compound itself into the soil.
The method of treatment according to the invention also provides the use of compounds ((A) and (B)) or ((A) and (B) and (C)) in a simultaneous, or sequential manner.
The dose of active compound/application rate usually applied in the method of treatment according to the invention is generally and advantageously

    
    
        for foliar treatments: from 0.1 to 10,000 g/ha, preferably from 10 to 1,000 g/ha, more preferably from 25 to 300 g/ha; in case of drench or drip application, the dose can even be reduced, especially while using inert substrates like rockwool or perlite;
        for seed treatment: from 2 to 200 g per 100 kilogram of seed, preferably from 3 to 150 g per 100 kilogram of seed;
        for soil treatment: from 0.1 to 10,000 g/ha, preferably from 1 to 5,000 g/ha.
    
    


The doses herein indicated are given as illustrative examples of the method according to the invention. A person skilled in the art will know how to adapt the application doses, notably according to the nature of the plant or crop to be treated.
The combination according to the invention can be used in order to protect plants within a certain time range after the treatment against pests and/or phytopathogenic fungi and/or microorganisms and/or pests. The time range, in which protection is effected, spans in general one to 28 days, preferably one to 14 days after the treatment of the plants with the combinations or up to 200 days after the treatment of plant propagation material.
The method of treatment according to the invention may also be useful to treat propagation material such as tubers, in particular potato tubers or rhizomes, but also seeds, seedlings or seedlings pricking out and plants or plants pricking out. This method of treatment can also be useful to treat roots. The method of treatment according to the invention can also be useful to treat the over-ground parts of the plant such as trunks, stems or stalks, leaves, flowers and fruit of the concerned plant.
A further aspect of the present invention is a method of protecting natural substances of vegetable or animal origin or their processed forms, which have been taken from the natural life cycle, which comprises applying to said natural substances of vegetable or animal origin or their processed forms a combination of compounds (A) and (B) in a synergistically effective amount.
A preferred embodiment is a method of protecting natural substances of vegetable origin or their processed forms, which have been taken from the natural life cycle, which comprises applying to said natural substances of vegetable origin or their processed forms a combination of compounds (A), (B) and (C) in a synergistically effective amount.
A further preferred embodiment is a method of protecting fruit, preferably pomes, stone fruits, soft fruits and citrus fruits, or their processed forms, which have been taken from the natural life cycle, which comprises applying to said natural substances of vegetable origin or their processed forms a combination of compounds (A) and (B) in a synergistically effective amount.
A further preferred embodiment is a method of protecting fruit, preferably pomes, stone fruits, soft fruits and citrus fruits, or their processed forms, which have been taken from the natural life cycle, which comprises applying to said natural substances of vegetable origin or their processed forms a combination of compounds (A), (B) and (C) in a synergistically effective amount.
The invention comprises a procedure in which the seed is treated simultaneously (i.e. at the same time) with a compound ((A) and (B)) or ((A) and (B) and (C)). It further comprises a method in which the seed is treated with compound ((A) and (B)) or ((A) and (B) and (C)) sequentially (i.e. separately).
The invention also comprises a seed, which has been treated with a compound ((A) and (B)) or ((A) and (B) and (C)) simultaneously (i.e. at the same time). The invention also comprises a seed, which has been treated with a compound ((A) and (B)) or ((A) and (B) and (C)) sequentially (i.e. separately). For the latter seed, the active ingredients can be applied in separate layers. These layers can optionally be separated by an additional layer that may or may not contain an active ingredient.
The combinations and/or compositions of the invention are particularly suitable for the treatment of seeds. A large part of the damage caused by pests and/or phytopathogenic fungi and/or microorganisms on cultigens occurs by infestation of the seed during storage and after sowing the seed in the ground as well as during and after germination of the plants. This phase is especially critical since the roots and shoots of the growing plant are particularly sensitive and even a small amount of damage can lead to withering of the whole plant. There is therefore considerable interest in protecting the seed and the germinating plant by the use of suitable agents.
The control of pests and/or phytopathogenic fungi and/or microorganisms by treatment of the seeds of plants or plant propagation material has been known for a considerable time and is the object of continuous improvement. However, there are a number of problems in the treatment of seed that cannot always be satisfactorily solved. Therefore it is worthwhile to develop methods for the protection of seeds or germinating plants or plant propagation material which makes the additional application of plant protection agents after seeding or after germination of the plants unnecessary. It is further worthwhile to optimize the amount of the applied active material such that the seed and the germinating plants are protected against infestation by pests and/or phytopathogenic fungi and/or microorganisms as best as possible without the plants themselves being damaged by the active compound applied. In particular, methods for the treatment seed should also take into account the intrinsic fungicidal and/or insecticidal properties of transgenic plants in order to achieve optimal protection of the seed and germinating plants with a minimal expenditure of plant protection agents.
The present invention relates therefore especially to a method for the protection of seed and germinating plants from infestation with pests and/or phytopathogenic fungi and/or microorganisms in that the seed is treated with the combination/composition of the invention. In addition the invention relates also to the use of the combination/composition of the invention for the treatment seed for protection of the seed and the germinating plants from pests and/or phytopathogenic fungi and/or microorganisms. Furthermore the invention relates to seed which was treated with a combination/composition of the invention for protection from pests and/or phytopathogenic fungi and/or microorganisms.
One of the advantages of the invention is because of the special systemic properties of the combination/composition of the invention treatment with this combination/composition protect not only the seed itself from pests and/or phytopathogenic fungi and/or microorganisms but also the plants emerging after sprouting. In this way the direct treatment of the culture at the time of sowing or shortly thereafter can be omitted.
A further advantage is the synergistic increase in fungicidal and/or insecticidal activity of the combination/composition of the invention in comparison to the respective individual active compounds, which extends beyond the sum of the activity of both individually applied active compounds. In this way an optimization of the amount of active compound applied is made possible.
It is also be regarded as advantageous that the mixtures of the invention can also be used in particular with transgenic seeds or plant propagation material whereby the plants emerging from this seed are capable of the expression of a protein directed against pests and phytopathogenic fungi and/or microorganisms. By treatment of such seed with the agents of the invention certain pests and/or phytopathogenic fungi and/or microorganisms can already be controlled by expression of the, for example, insecticidal protein, and it is additionally surprising that a synergistic activity supplementation occurs with the agents of the invention, which improves still further the effectiveness of the protection from pest infestation.
The agents of the invention are suitable for the protection of seed of plant varieties of all types as already described which are used in agriculture, in greenhouses, in forestry, in furrow treatment, in horticulture or in vineyards. In particular, this concerns seed of cereals (like wheat, barley, rye, triticale, millet, oats, rice), maize, cotton, soya bean, potato, sunflower, beans, coffee, beet (e.g. sugar beet, mangold and feed beet), peanut, canola, rapeseed, poppy, olive, coconut, cacao, sugar cane or tobacco. The combination/compositions of the invention are also suitable for the treatment of the seed of fruit plants and vegetables (like tomato, cucumber, onion and lettuce), lawn, turf and ornamental plants as previously described. Particular importance is attached to the treatment of the seed of wheat, barley, rye, triticale, oats, maize, rice, soya bean, cotton, canola and rape.
As already described, the treatment of transgenic seed with a combination/composition of the invention is of particular importance. This concerns the seeds or plant propagation material of plants which generally contain at least one heterologous gene that controls the expression of a polypeptide with special insecticidal properties. The heterologous gene in transgenic seed can originate from microorganisms such as Bacillus, Rhizobium, Pseudomonas, Serratia, Trichoderma, Clavibacter, Glomus or Gliocladium. The present invention is particularly suitable for the treatment of transgenic seed that contains at least one heterologous gene that originates from Bacillus sp. and whose gene product exhibits activity against the European corn borer and/or western corn rootworm. Particularly preferred is a heterologous gene that originates from Bacillus thuringiensis. 
Within the context of the present invention the combination/composition of the invention is applied to the seed alone or in a suitable formulation. Preferably the seed is handled in a state in which it is so stable, that no damage occurs during treatment. In general treatment of the seed can be carried out at any time between harvest and sowing. Normally seed is used that was separated from the plant and has been freed of spadix, husks, stalks, pods, wool or fruit flesh. Use of seed that was harvested, purified, and dried to moisture content of below 15% w/w. Alternative, seed treated with water after drying and then dried again can also be used.
In general care must be taken during the treatment of the seed that the amount of the combination/composition of the invention and/or further additive applied to the seed is so chosen that the germination of the seed is not impaired and the emerging plant is not damaged. This is to be noted above all with active compounds which can show phytotoxic effects when applied in certain amounts.
The combination/compositions of the invention can be applied directly, that is without containing additional components and without being diluted. It is normally preferred to apply the combination/composition to the seed in the form of a suitable formulation. Suitable formulations and methods for seed treatment are known to the person skilled in the art and are described, for example, in the following documents: U.S. Pat. No. 4,272,417 A, U.S. Pat. No. 4,245,432 A, U.S. Pat. No. 4,808,430 A, U.S. Pat. No. 5,876,739 A, US 2003/0176428 A1, WO 2002/080675 A1, WO 2002/028186 A2.
The active compound combinations and compositions which can be used according to the invention can be converted into customary seed dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries or other coating materials for seed, and also ULV formulations.
These formulations are prepared in a known manner by mixing the active compounds or active compound combinations with customary additives, such as, for example, customary extenders and also solvents or diluents, colorants, wetting agents, dispersants, emulsifiers, defoamers, preservatives, secondary thickeners, adhesives, gibberellins and optionally water as well.
Suitable colorants that may be present in the seed dressing formulations of the invention include all colorants customary for such purposes. Use may be made both of pigments, of sparing solubility in water, and of dyes, which are soluble in water. Examples that may be mentioned include the colorants known under the designations rhodamine B, C.I. Pigment Red 112, and C.I. Solvent Red 1.
Suitable wetting agents that may be present in the seed dressing formulations of the invention include all substances which promote wetting and are customary in the formulation of active agrochemical substances. With preference it is possible to use alkylnaphthalene-sulphonates, such as diisopropyl- or diisobutylnaphthalene-sulphonates.
Suitable dispersants and/or emulsifiers that may be present in the seed dressing formulations of the invention include all nonionic, anionic, and cationic dispersants which are customary in the formulation of active agrochemical substances as outlined above.
Suitable defoamers that may be present in the seed dressing formulations of the invention include all foam-inhibiting substances which are customary in the formulation of active agrochemical substances. With preference it is possible to use silicone defoamers and magnesium stearate.
Suitable preservatives that may be present in the seed dressing formulations of the invention include all substances which can be used for such purposes in agrochemical compositions. By way of example, mention may be made of dichlorophen and benzyl alcohol hemiformal.
Suitable secondary thickeners that may be present in the seed dressing formulations of the invention include all substances which can be used for such purposes in agrochemical compositions. Preferred suitability is possessed by cellulose derivatives, acrylic acid derivatives, xanthan, modified clays, and highly dispersed silica.
Suitable adhesives that may be present in the seed dressing formulations of the invention include all customary binders which can be used in seed dressing. With preference, mention may be made of polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose.
Suitable gibberellins that may be present in the seed dressing formulations of the invention include preferably gibberelin A1, A3 (=gibberellinic acid), A4, and A7, particular preferably gibberelin A3 (=gibberellinic acid). The gibberellins of the formula (II) are known, the nomenclature of the gibberlins can be found the reference mentioned below (cf. R. Wegler “Chemie der Pflanzenschutz- and Schädlingsbekämpfungsmittel”, Volume 2, Springer Verlag, Berlin-Heidelberg-New York, 1970, pages 401-412).
Suitable mixing equipment for treating seed with the seed dressing formulations to be used according to the invention or the preparations prepared from them by adding water includes all mixing equipment which can commonly be used for dressing. The specific procedure adopted when dressing comprises introducing the seed into a mixer, adding the particular desired amount of seed dressing formulation, either as it is or following dilution with water beforehand, and carrying out mixing until the formulation is uniformly distributed on the seed. Optionally, a drying operation follows.
The active compound combination, having good plant compatibility and favourable homeotherm toxicity, is suitable for controlling animal pests, in particular insects, arachnids and nematodes, encountered in agriculture, in forests, in the protection of stored products and materials and in the hygiene sector. It is preferably used as crop protection composition for foliar and soil treatment.
It is effective against normally sensitive and resistant species and against all or individual stages of development. The abovementioned pests include:
From the order of the Isopoda, for example, Oniscus asellus, Armadillidium vulgare, Porcellio scaber. From the order of the Diplopoda, for example, Blaniulus guttulatus. From the order of the Chilopoda, for example, Geophilus carpophagus, Scutigera spp. From the order of the Symphyla, for example, Scutigerella immaculata. From the order of the Thysanura, for example, Lepisma saccharina. From the order of the Collembola, for example, Onychiurus armatus. From the order of the Orthoptera, for example, Acheta domesticus, Gryllotalpa spp., Locusta migratoria migratorioides, Melanoplus spp., Schistocerca gregaria. From the order of the Blattaria, for example, Blatta orientalis, Periplaneta americana, Leucophaea maderae, Blattella germanica. From the order of the Dermaptera, for example, Forficula auricularia. From the order of the Isoptera, for example, Reticulitermes spp. From the order of the Phthiraptera, for example, Pediculus humanus corporis, Haematopinus spp., Linognathus spp., Trichodectes spp., Damalinia spp. From the order of the Thysanoptera, for example, Hercinothrips femoralis, Thrips tabaci, Thrips palmi, Frankliniella occidentalis. From the order of the Heteroptera, for example, Eurygaster spp., Dysdercus intermedius, Piesma quadrata, Cimex lectularius, Rhodnius prolixus, Triatoma spp. From the order of the Homoptera, for example, Aleurodes brassicae, Bemisia tabaci, Trialeurodes vaporariorum, Aphis gossypii, Brevicoryne brassicae, Cryptomyzus ribis, Aphis fabae, Aphis pomi, Eriosoma lanigerum, Hyalopterus arundinis, Phylloxera vastatrix, Pemphigus spp., Macrosiphum avenae, Myzus spp., Phorodon humuli, Rhopalosiphum padi, Empoasca spp., Euscelis bilobatus, Nephotettix cincticeps, Lecanium corni, Saissetia oleae, Laodelphax striatellus, Nilaparvata lugens, Aonidiella aurantii, Aspidiotus hederae, Pseudococcus spp., Psylla spp. From the order of the Lepidoptera, for example, Pectinophora gossypiella, Bupalus piniarius, Chematobia brumata, Lithocolletis blancardella, Hyponomeuta padella, Plutella xylostella, Malacosoma neustria, Euproctis chrysorrhoea, Lymantria spp., Bucculatrix thurberiella, Phyllocnistis citrella, Agrotis spp., Euxoa spp., Feltia spp., Earias insulana, Heliothis spp., Mamestra brassicae, Panolis flammea, Spodoptera spp., Trichoplusia ni, Carpocapsa pomonella, Pieris spp., Chilo spp., Pyrausta nubilalis, Ephestia kuehniella, Galleria mellonella, Tineola bisselliella, Tinea pellionella, Hofmannophila pseudospretella, Cacoecia podana, Capua reticulana, Choristoneura fumiferana, Clysia ambiguella, Homona magnanima, Tortrix viridana, Cnaphalocerus spp., Oulema oryzae. From the order of the Coleoptera, for example, Anobium punctatum, Rhizopertha dominica, Bruchidius obtectus, Acanthoscelides obtectus, Hylotrupes bajulus, Agelastica alni, Leptinotarsa decemlineata, Phaedon cochleariae, Diabrotica spp., Psylliodes chrysocephala, Epilachna varivestis, Atomaria spp., Oryzaephilus surinamensis, Anthonomus spp., Sitophilus spp., Otiorrhynchus sulcatus, Cosmopolites sordidus, Ceuthorrhynchus assimilis, Hypera postica, Dermestes spp., Trogodenna spp., Anthrenus spp., Attagenus spp., Lyctus spp., Meligethes aeneus, Ptinus spp., Niptus hololeucus, Gibbium psylloides, Tribolium spp., Tenebrio molitor, Agriotes spp., Conoderus spp., Melolontha melolontha, Amphimallon solstitialis, Costelytra zealandica, Lissorhoptrus oryzophilus. From the order of the Hymenoptera, for example, Diprion spp., Hoplocampa spp., Lasius spp., Monomorium pharaonic, Vespa spp. From the order of the Diptera, for example, Aedes spp., Anopheles spp., Culex spp., Drosophila melanogaster, Musca spp., Fannia spp., Calliphora erythrocephala, Lucilia spp., Chrysomyia spp., Cuterebra spp., Gastrophilus spp., Hyppobosca spp., Stomoxys spp., Oestrus spp., Hypoderma spp., Tabanus spp., Tannia spp., Bibio hortulanus, Oscinella frit, Phorbia spp., Pegomyia hyoscyami, Ceratitis capitata, Dacus oleae, Tipula paludosa, Hylemyia spp., Liriomyza spp. From the order of the Siphonaptera, for example, Xenopsylla cheopis, Ceratophyllus spp. From the order of the Arachnida, for example, Scorpio maurus, Latrodectus mactans, Acarus siro, Argas spp., Ornithodoros spp., Dermanyssus gallinae, Eriophyes ribis, Phyllocoptruta oleivora, Boophilus spp., Rhipicephalus spp., Amblyomma spp., Hyalomma spp., Ixodes spp., Psoroptes spp., Chorioptes spp., Sarcoptes spp., Tarsonemus spp., Bryobia praetiosa, Panonychus spp., Tetranychus spp., Hemitarsonemus spp., Brevipalpus spp.
The plant-parasitic nematodes include, for example, Pratylenchus spp., Radopholus similis, Ditylenchus dipsaci, Tylenchulus semipenetrans, Heterodera spp., Globodera spp., Meloidogyne spp., Aphelenchoides spp., Longidorus spp., Xiphinema spp., Trichodorus spp., Bursaphelenchus spp.
Among the diseases of plants or crops that can be controlled by the method according to the invention, mention may be made of:
Powdery Mildew diseases such as
Blumeria diseases caused for example by Blumeria graminis 
Podosphaera diseases caused for example by Podosphaera leucotricha 
Sphaerotheca diseases caused for example by Sphaerotheca fuliginea 
Uncinula diseases caused for example by Uncinula necator 
Rust diseases such as
Gymnosporangium diseases caused for example by Gymnosporangium sabinae 
Hemileia diseases caused for example by Hemileia vastatrix 
Phakopsora diseases caused for example by Phakopsora pachyrhizi and Phakopsora meibomiae 
Puccinia diseases caused for example by Puccinia recondite, and Puccinia triticina; 
Uromyces diseases caused for example by Uromyces appendiculatus 
Oomycete diseases such as
Bremia diseases caused for example by Bremia lactucae 
Peronospora diseases caused for example by Peronospora pisi and Peronospora brassicae 
Phytophthora diseases caused for example by Phytophthora infestans 
Plasmopara diseases caused for example by Plasmopara viticola 
Pseudoperonospora diseases caused for example by Pseudoperonospora humuli and Pseudoperonospora cubensis 
Pythium diseases caused for example by Pythium ultimum 
Leafspot, Leaf blotch and Leaf blight diseases such as
Alternaria diseases caused for example by Alternaria solani 
Cercospora diseases caused for example by Cercospora beticola 
Cladiosporium diseases caused for example by Cladiosporium cucumerinum 
Cochliobolus diseases caused for example by Cochliobolus sativus 
(Conidiaform: Drechslera, Syn: Helminthosporium);
Colletotrichum diseases caused for example by Colletotrichum lindemuthianum 
Cycloconium diseases caused for example by Cycloconium oleaginum 
Diaporthe diseases caused for example by Diaporthe citri 
Elsinoe diseases caused for example by Elsinoe fawcettii 
Gloeosporium diseases caused for example by Gloeosporium laeticolor
Glomerella diseases caused for example by Glomerella cingulata 
Guignardia diseases caused for example by Guignardia bidwellii 
Leptosphaeria diseases caused for example by Leptosphaeria maculans 
Magnaporthe diseases caused for example by Magnaporthe grisea 
Mycosphaerella diseases caused for example by Mycosphaerella graminicola, Mycosphaerella fijiensis, and Mycosphaerella musicola 
Phaeosphaeria diseases caused for example by Phaeosphaeria nodorum 
Pyrenophora diseases caused for example by Pyrenophora teres 
Ramularia diseases caused for example by Ramularia collo-cygni 
Rhynchosporium diseases caused for example by Rhynchosporium secalis 
Septoria diseases caused for example by Septoria apii; 
Typhula diseases caused for example by Thyphula incarnata 
Venturia diseases caused for example by Venturia inaequalis 
Root- and Stem diseases such as
Corticium diseases caused for example by Corticium graminearum 
Fusarium diseases caused for example by Fusarium oxysporum 
Gaeumannomyces diseases caused for example by Gaeumannomyces graminis 
Rhizoctonia diseases caused for example by Rhizoctonia solani 
Oculimacula (Tapesia) diseases caused for example by Oculimacula Tapesia acuformis 
Thielaviopsis diseases caused for example by Thielaviopsis basicola 
Ear and Panicle diseases including Maize cob such as
Alternaria diseases caused for example by Alternaria spp.
Aspergillus diseases caused for example by Aspergillus flavus 
Cladosporium diseases caused for example by Cladiosporium cladosporioides 
Claviceps diseases caused for example by Claviceps purpurea 
Fusarium diseases caused for example by Fusarium culmorum 
Gibberella diseases caused for example by Gibberella zeae 
Monographella diseases caused for example by Monographella nivalis 
Smut- and Bunt diseases such as
Sphacelotheca diseases caused for example by Sphacelotheca reiliana 
Tilletia diseases caused for example by Tilletia caries 
Urocystis diseases Urocystis occulta 
Ustilago diseases caused for example by Ustilago nuda; 
Fruit Rot and Mould diseases such as
Aspergillus diseases caused for example by Aspergillus flavus 
Botrytis diseases caused for example by Botrytis cinerea 
Penicillium diseases caused for example by Penicillium expansum and Penicillium purpurogenum 
Sclerotinia diseases caused for example by Sclerotinia sclerotiorum; 
Verticillium diseases caused for example by Verticillium alboatnim 
Seed- and Soilborne Decay, Mould, Wilt, Rot and Damping-off diseases
Alternaria diseases, caused for example by Alternaria brassicicola 
Aphanomyces diseases, caused for example by Aphanomyces euteiches 
Ascochyta diseases, caused for example by Ascochyta lentis 
Aspergillus diseases, caused for example by Aspergillus flavus 
Cladosporium diseases, caused for example by Cladosporium herbarum 
Cochliobolus diseases, caused for example by Cochliobolus sativus 
(As conidia: Drechslera, Bipolaris Syn: Helminthosporium);
Colletotrichum diseases, caused for example by Colletotrichum coccodes; 
Fusarium diseases, caused for example by Fusarium culmorum; 
Gibberella diseases, caused for example by Gibberella zeae; 
Macrophomina diseases, caused for example by Macrophomina phaseolina 
Monographella diseases, caused for example by Monographella nivalis; 
Penicillium diseases, caused for example by Penicillium expansum 
Phoma diseases, caused for example by Phoma lingam 
Phomopsis diseases, caused for example by Phomopsis sojae; 
Phytophthora diseases, caused for example by Phytophthora cactorum; 
Pyrenophora diseases, caused for example by Pyrenophora graminea 
Pyricularia diseases, caused for example by Pyricularia oryzae; 
Pythium diseases, caused for example by Pythium ultimum; 
Rhizoctonia diseases, caused for example by Rhizoctonia solani; 
Rhizopus diseases, caused for example by Rhizopus oryzae
Sclerotium diseases, caused for example by Sclerotium rolfsii; 
Septoria diseases, caused for example by Septoria nodorum; 
Typhula diseases, caused for example by Typhula incarnata; 
Verticillium diseases, caused for example by Verticillium dahliae 
Canker, Broom and Dieback diseases such as
Nectria diseases caused for example by Nectria galligena 
Blight diseases such as
Monilinia diseases caused for example by Monilinia laxa 
Leaf Blister or Leaf Curl diseases including deformation of blooms and fruits such as
Taphrina diseases caused for example by Taphrina deformans 
Decline diseases of wooden plants such as
Esca disease caused for example by Phaeomoniella clamydospora and Phaeoacremonium aleophilum and Fomitiporia mediterranea 
Diseases of Flowers and Seeds such as
Botrytis diseases caused for example by Botrytis cinerea 
Diseases of Tubers such as
Rhizoctonia diseases caused for example by Rhizoctonia solani 
Helminthosporium diseases caused for example by Helminthosporium solani 
Diseases caused by Bacterial Organisms such as
Xanthomanas species for example Xanthomonas campestris pv. Oryzae 
Pseudomonas species for example Pseudomonas syringae pv. Lachrymans Erwinia species for example Erwinia amylovora. 
Fungal diseases of the foliage, upper stems, pods and seeds for example
Alternaria leaf spot (Alternaria spec. atrans tenuissima), Anthracnose (Colletotrichum gloeosporoides dematium var. truncatum), Brown spot (Septoria glycines), Cercospora leaf spot and blight (Cercospora kikuchii), Choanephora leaf blight (Choanephora infundibulifera trispora (Syn.)), Dactuliophora leaf spot (Dactuliophora glycines), Downy Mildew (Peronospora manshurica), Drechslera blight (Drechslera glycini), Frogeye Leaf spot (Cercospora sojina), Leptosphaerulina Leaf Spot (Leptosphaerulina trifolii), Phyllostica Leaf Spot (Phyllosticta sojaecola), Pod and Stern blight (Phomopsis sojae), Powdery Mildew (Microsphaera diffusa), Pyrenochaeta Leaf Spot (Pyrenochaeta glycines), Rhizoctonia Aerial, Foliage, and Web blight (Rhizoctonia solani), Rust (Phakopsora pachyrhizi, Phakopsora meibomiae), Scab (Sphaceloma glycines), Stemphylium Leaf blight (Stemphylium botryosum), Target Spot (Corynespora cassiicola)

Fungal Disease of the Roots and Lower Stems for Example

Black Root Rot (Calonectria crotalariae), Charcoal Rot (Macrophomina phaseolina), Fusarium blight or Wilt, Root Rot, and Pod and Collar Rot (Fusarium oxysporum, Fusarium orthoceras, Fusarium semitectum, Fusarium equiseti), Mycoleptodiscus Root Rot (Mycoleptodiscus terrestris), Neocosmospora (Neocosmopspora vasinfecta), Pod and Stern Blight (Diaporthe phaseolorum), Stem Canker (Diaporthe phaseolorum var. caulivora), Phytophthora Rot (Phytophthora megaspenna), Brown Stern Rot (Phialophora gregata), Pythium Rot (Pythium aphanidermatum, Pythium irregulare, Pythium debaryanum, Pythium myriotylum, Pythium ultimum), Rhizoctonia Root Rot, Stem Decay, and Damping-Off (Rhizoctonia solani), Sclerotinia Stem Decay (Sclerotinia sclerotiorum), Sclerotinia Southern Blight (Sclerotinia rolfsii), Thielaviopsis Root Rot (Thielaviopsis basicola).
Examples of fungi which cause damage to plants of the Musaceae family are
Acremonium spp.
Acrodontium simplex
Armillaria subspecies, for example Armillaria mellea, Armillaria tabescens 
Botryosphaeria ribis 
Cercospora hayi 
Ceratocystis paradoxa anamorph: Chalara paradoxa 
Cladosporium musae 
Colletotrichum musae 
Cordana johnstonii, Cordana musae 
Curvularia eragrostidis 
Cylindrocladium spp.
Cylindrocarpon musae 
Deightoniella torulosa 
Drechslera musae-sapientum 
Fusarium spp, for example Fusarium pallidoroseum, Fusarium solani anamorph Nectria haematococca, Fusarium oxysporum, Fusarium moniliforme teleomorph: Gibberella fujikuroi 
Fusarium oxysporum Schldl.:Fr. f. sp. cubense 
Guignardia musae Racib. anamorph: Phyllosticta musarum (Cooke)
Haplobasidion musae 
Junghuhnia vincta 
Lasiodiplodia theobromae 
Leptosphaeria musarum, 
Limacinula tenuis 
Marasmiellus inoderma 
Marasmius semiustus 
Mycosphaerella spp., for example Mycosphaerella musa, Mycosphaerella fijiensis anamorph:
Paracercospora fijiensis, Mycosphaerella musicola anamorph: Nattrassia mangiferae 
Pseudocercospora musae 
Nattrassia mangiferae or Hendersonula toruloidea 
Nectria foliicola 
Nigrospora sphaerica 
Pestalotiopsis leprogena 
Pestalotiopsis palmarum 
Pestalotiopsis disseminate
Phaeoseptoria musa 
Phyllachora musicola 
Pratylenchus coffeae 
Pratylenchus goodeyi 
Pratylenchus brachyurus 
Pratylenchus reniformia 
Pyricularia grisea 
Ramichloridium musa, Veronaea musa, Periconiella musae 
Rhizoctonia spp.
Rosellinia bunodes 
Sclerotinia sclerotiorum 
Trachysphaera fructigena 
Verticillium theobromae 
Uredo musae 
Uromyces musae 
Examples of bacteria which cause damage to plants of the Musaceae family are
Erwinia carotovora 
Pseudomonas spp., for example Pseudomonas solanacearum, for example Race 1, Race 2
Xanthomonas campestris 
Examples of viruses which cause damage to plants of the Musaceae family are
Banana bract mosaic virus
Banana bunchy top virus
Banana streak virus
Cucumber mosaic virus
Examples of nematodes which cause damage to plants of the Musaceae family are Radopholus spp. and Helicotylenchus spp.
Furthermore combinations and compositions according to the invention may also be used to reduce the contents of mycotoxins in plants and the harvested plant material and therefore in foods and animal feed stuff made therefrom.
In a preferred embodiment diseases of plants or crops that can be controlled by the method according to the invention are:
Oomycete diseases such as
Peronospora diseases caused for example by Peronospora pisi and Peronospora brassicae
Phytophthora diseases caused for example by Phytophthora infestans 
Plasmopara diseases caused for example by Plasmopara viticola 
Especially but not exclusively the following mycotoxins can be specified:
Deoxynivalenole (DON), Nivalenole, 15-Ac-DON, 3-Ac-DON, T2-und HT2-Toxins, Fumonisines, Zearalenone Moniliformine, Fusarine, Diaceotoxyscirpenole (DAS), Beauvericine, Enniatine, Fusaroproliferine, Fusarenole, Ochratoxines, Patuline, Ergotalkaloides und Aflatoxines, which are caused for example by the following fungal diseases: Fusarium spec., like Fusarium acuminatum, F. avenaceum, F. crookwellense, F. culmorum, F. graminearum (Gibberella zeae), F. equiseti, F. fiijikoroi, F. musarum, E oxysporum, F proliferatum, F. poae, F. pseudograminearum, F. sambucinum, F. scirpi, F. semitectum, F. solani, F. sporotrichoides, F. langsethiae, F. subglutinans, F. tricinctum, F. verticillioides and others but also by Aspergillus spec., Penicillium spec., Claviceps purpurea, Stachybotrys spec. and others.
The very good fungicidal effect of the combinations or compositions according to the invention is shown in the following example. While the single active compounds do show weaknesses in their fungicidal efficacy, the combinations or compositions show an effect which is greater than the single addition of the efficacies of each compound.
A synergistic effect for fungicides or insecticides is always present when the fungicidal/insecticidal action of the active compound combinations exceeds the expected action of the active compounds. The expected fungicidal/insecticidal action for a given combination of two or three active compounds can be calculated as follows, according to S. R. Colby (“Calculating Synergistic and Antagonistic Responses of Herbicide Combinations”, Weeds 1967, 15, 20-22):
If


    X is the efficacy observed for compound (A) at a defined dose (m ppm),
    Y is the efficacy observed for compound (B) at a defined dose (n ppm),
    Z is the efficacy observed for compound (C) at a defined dose (r ppm),
    E1 is the efficacy observed for compound (A) and compound (B) together at defined doses of m and n ppm,
    E2 is the efficacy observed for compound (A) and compound (B) and compound (C) together at defined doses of m, n and r ppm,

the Colby formula can be defined as shown below for a binary mixture:







  
    E
    1
  
  =
  
    X
    +
    Y
    -
    
      
        X
        ·
        Y
      
      100
    
  




and for a ternary mixture:





  
    E
    2
  
  =
  
    X
    +
    Y
    +
    Z
    -
    
      (
      
        
          
            X
            ·
            Y
          
          +
          
            X
            ·
            Z
          
          +
          
            Y
            ·
            Z
          
        
        100
      
      )
    
    +
    
      
        X
        ·
        Y
        ·
        Z
      
      10000
    
  




The efficacies are calculated as %. 0% efficacy is corresponding to the non-treated control, while an efficacy of 100% implies that no infection at all can be observed.
If the actual fungicidal/insecticidal activity exceeds the calculated value, then the activity of the combination is superadditive, i.e. a synergistic effect exists. In this case, the efficacy which was actually observed must be greater than the value for the expected efficacy (E) calculated from the abovementioned formula.
The term “synergistic effect” also means the effect defined by application of the Tammes method, “Isoboles, a graphic representation of synergism in pesticides”, Netherlands Journal of Plant Pathology, 70 (1964), pages 73-80.
The invention is illustrated by the examples below. The invention is not restricted to the examples only.


EXAMPLE A
Fusarium nivale (Var. majus)-Test (Wheat)/Preventive
Solvent: 49 parts by weight of n,n-dimethylacetamid
Emulsifier: 1 part by weight of alkylaryl polyglycol ether
To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application.
After the spray coating has been dried, the plants are slightly injured by using a sandblast and afterwards they are sprayed with a conidia suspension of Fusarium nivale (var. majus).
The plants are placed in the greenhouse under a translucent incubation cabinet at a temperature of approximately 10° C. and a relative atmospheric humidity of approximately 100%.
The test is evaluated 5 days after the inoculation. 0% means an efficacy which corresponds to that of the control, while an efficacy of 100% means that no disease is observed.
The table below clearly shows that the observed activity of the active compound combination according to the invention is greater than the calculated activity, i.e. a synergistic effect is present.







TABLE A







Fusarium nivale (var. majus)-test (wheat)/preventive












Application 




Ratio of
rate of active
Efficacy in %











Active compounds
mixture
compound in g/ha
found*
calc.**





Ex. A Isotianil 




1000
25



 


Ex. B Spirodiclofen 




250
13



 












Ex. A Isotianil +
 {close oversize brace} 
4:1
1000 + 250
50
35


Ex. B Spirodiclofen







 











Ex. A Isotianil 




1000
25



 


Ex. B Spiromesifen 




250
38



 












Ex. A Isotianil +
 {close oversize brace} 
4:1
1000 + 250
63
54


Ex. B Spirodiclofen





*found = activity found


**calc. = activity calculated using Colby's formula






EXAMPLE B
Septoria tritici-Test (Wheat)/Preventive
Solvent: 49 parts by weight of n,n-dimethylacetamid
Emulsifier: 1 part by weight of alkylaryl polyglycol ether
To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application.
After the spray coating has been dried, the plants are sprayed with a spore suspension of Septoria tritici. The plants remain for 48 hours in an incubation cabinet at approximately 20° C. and a relative atmospheric humidity of approximately 100% and afterwards for 60 hours at approximately 15° C. in a translucent incubation cabinet at a relative atmospheric humidity of approximately 100%.
The plants are placed in the greenhouse at a temperature of approximately 15° C. and a relative atmospheric humidity of approximately 80%.
The test is evaluated 21 days after the inoculation. 0% means an efficacy which corresponds to that of the control, while an efficacy of 100% means that no disease is observed.
The table below clearly shows that the observed activity of the active compound combination according to the invention is greater than the calculated activity, i.e. a synergistic effect is present.







TABLE B







Septoria tritici-test (wheat)/preventive












Application 




Ratio of
rate of active
Efficacy in %











Active compounds
mixture
compound in g/ha
found*
calc.**





Ex. A Isotianil 




1000
33



 


Ex. B Spirotetramat 




250
22



 












Ex. A Isotianil +
 {close oversize brace} 
4:1
1000 + 250 
56
48


Ex. B Spirotetramat







 











Ex. A Isotianil 




500
33



 


Ex. B Spirodiclofen 




250
22



 


Ex. C Imidacloprid 




500
22



 












Ex. A Isotianil +

2:1:2
500 + 250 + 500
67
59


Ex. B Spirodiclofen +
 {close oversize brace} 






Ex. C Imidacloprid







 











Ex. A Isotianil 




500
33



 


Ex. B Spirotetramat 




250
22



 


Ex. C Imidacloprid 




1000
22



 












Ex. A Isotianil +

2:1:4
500 + 250 + 1000
67
59


Ex. B Spirotetramat +
 {close oversize brace} 






Ex. C Imidacloprid





*found = activity found


**calc. = activity calculated using Colby's formula






EXAMPLE C
Plutella xylostella Test
Solvent: 7 parts by weight of dimethylformamide
Emulsifier: 2 parts by weight of alkylaryl polyglycolether
To produce a suitable preparation of active compound, 1 part by weight of active compound is mixed with the stated amount of solvent and emulsifier, and the concentrate is diluted with emulsifier-containing water to the desired concentration.
Cabbage leaves (Brassica oleracea) are treated by being sprayed into the preparation of the active compound of the desired concentration and are infested with larvae of the diamondback moth (Plutella xylostella) as long as the leaves are still moist.
After the specified period of time, the mortality in % is determined. 100% means that all the caterpillars have been killed; 0% means that none of the caterpillars have been killed.
In this test, for example, the following combinations according to the invention show a superior level of efficacy compared to the single compounds:







TABLE C





Plutella xylostella - Test



















Concentration
Mortality



Active Ingredient
in ppm
in % after 4d





Isotianil
200
5



Spirodiclofen
20
15



Spiromesifen
500
25















obs.*
cal.**





Isotianil + Spirodiclofen
200 + 20 
40
19.25


(10:1)





According to the invention





Isotianil + Spiromesifen
200 + 500
70
28.75


(1:2.5)





According to the invention














Concentration
Mortality



Active Ingredient
in ppm
in % after 2d





Isotianil
200
5



Imidacloprid
100
20




20
0



Spirotetramat
20
20















obs.*
cal.**





Isotianil + Imidacloprid +
200 + 100 + 20
75
39.2


Spirotetramat
200 + 20 + 20
65
24


(10:5:1)





(10:1:1)





According to the invention





*obs. = observed insecticidal efficacy


**cal. = efficacy calculated with Colby-formula






EXAMPLE D
Tetranychus urticae Test
Solvent: 7 parts by weight of dimethylformamide
Emulsifier: 2 parts by weight of alkylaryl polyglycolether
To produce a suitable preparation of active compound, 1 part by weight of active compound is mixed with the stated amount of solvent and emulsifier, and the concentrate is diluted with emulsifier-containing water to the desired concentration.
French bean leaves (Phaseolus vulgaris) which are heavily infested with all stages of the two spotted spidermites (Tetranychus urticae) are treated by being sprayed into the preparation of the active compound of the desired concentration.
After the specified period of time, the mortality in % is determined. 100% means that all the spider mites have been killed; 0% means that none of the spider mites have been killed.
In this test, for example, the following combinations according to the invention show a superior level of efficacy compared to the single compounds:







TABLE D





Tetranychus urticae - Test



















Concentration
Mortality



Active Ingredient
in ppm
in % after 2d





Isotianil
200
0



Spirodiclofen
20
30















obs.*
cal.**





Isotianil + Spirodiclofen
200 + 20
50
30


(10:1)





According to the invention














Concentration
Mortality



Active Ingredient
in ppm
in % after 4d





Isotianil
200
0



Spiromesifen
100
75



Spirotetramat
20
55















obs.*
cal.**





Isotianil + Spiromesifen
 200 + 100
98
75


(2:1)





According to the invention





Isotianil + Spirotetramat
200 + 20
85
55


(10:1)





According to the invention





*obs. = observed insecticidal efficacy


**cal. = efficacy calculated with Colby-formula

## Claims

1. A composition comprising
(A) Isotianil;
(B) a first insecticidal active compound selected from the group consisting of spirotetramat, spirodiclofen and spiromesifen; and
(C) imidacloprid.

2. The composition according to claim 1 wherein the weight ratio between (A) Isotianil and (B) the first insecticidal active compound is 1:125 to 125:1.

3. The composition according to claim 1, wherein the weight ratio between (A) Isotianil and (B) the first insecticidal active compound is 1:25 to 25:1.

4. The composition according to claim 1, wherein the weight ratio between (A) Isotianil and (B) the first insecticidal active compound is 1:10 to 10:1.

5. The composition according to claim 1 wherein the weight ratio between any two components (A), (B), or (C), independently of each other, is 1:125 to 125:1.

6. The composition according to claim 1 further comprising adjuvants, solvents, carriers, surfactants or extenders.

7. A method for controlling phytopathogenic fungi and/or microorganisms and/or pests of plants or crops comprising applying the composition according to claim 1 to a seed, to plant propagation material, to a plant, to fruit of the plant, to soil in which the plant is growing, or to soil from which the seed, the plant propagation material, or the plant grows.

8. The method according to claim 7 wherein the composition according to claim 1 is applied at a rate of from 0.1 g/ha to 10 kg/ha for foliar treatment; at a rate of from 0.1 g/ha to 10 kg/ha for soil treatment; or at a rate of from 2 to 200 g/100 kg of seed for seed treatment.

9. The method according to claim 7 wherein the composition according to claim 1 is applied to seed.

10. The method according to claim 9 wherein the seed is transgenic seed.

11. A method for protecting a seed and/or shoots and foliage of a plant grown from the seed from damage by a pest or a fungus, comprising treating an unsown seed with the composition according to claim 1.

12. The composition according to claim 1 further comprising seed.

13. A method for controlling phytopathogenic fungi and/or microorganisms and/or pests of plants or crops, comprising applying
(A) Isotianil;
(B) a first insecticidal active compound selected from the group consisting of spirotetramat, spirodiclofen and spiromesifen; and
(C) imidacloprid,

to a seed, to plant propagation material, to a plant, to fruit of the plant, or to soil in which the plant is growing, or to soil from which the seed, the plant propagation material, or the plant grows, wherein (A), (B), and (C) are applied simultaneously or sequentially.

14. A method for protecting a seed and/or shoots and foliage of a plant grown from the seed from damage by a pest or a fungus, comprising treating an unsown seed with
(A) Isotianil;
(B) a first insecticidal active compound selected from the group consisting of spirotetramat, spirodiclofen and spiromesifen; and
(C) imidacloprid,

wherein the unsown seed is treated simultaneously with (A), (B), and (C).

15. A method for protecting a seed and/or shoots and foliage of a plant grown from the seed from damage by a pest or a fungus, comprising treating an unsown seed with
(A) Isotianil;
(B) a first insecticidal active compound selected from the group consisting of spirotetramat, spirodiclofen and spiromesifen; and
(C) imidacloprid,

wherein the unsown seed is treated sequentially with (A), (B), and (C).