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ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Molecular Plant Pathology Laboratory Docs







Rose Additional Publicationsheadline bar **Antimicrobial activity of bacteriophage derived triple fusion protein against Staphylococcus aureus.** Kovalskaya, N., Herndon, E., Foster-Frey, J. A., Donovan, D. M., Hammond, R. W.  2019.  **Pospiviroid Infection of Tomato Regulates the Expression of Genes Involved in Flower and Fruit Development.** Aviña-Padilla K, Rivera-Bustamante R, Kovalskaya NY, Hammond RW. Viruses. 2018 Sep 21;10(10). pii: E516. doi: 10.3390/v10100516. **PMID:** 30241423  **Characterization of alfalfa virus F, a new member of the genus Marafivirus.** Nemchinov LG, François S, Roumagnac P, Ogliastro M, Hammond RW, Mollov DS, Filloux D. PLoS One. 2018 Sep 4;13(9):e0203477. doi: 10.1371/journal.pone.0203477. eCollection 2018. **PMID:** 30180217  **Engineering resistance against viroids.** Flores R, Navarro B, Kovalskaya N, Hammond RW, Di Serio F. Curr Opin Virol. 2017 Oct;26:1-7. doi: 10.1016/j.coviro.2017.07.003. Epub 2017 Jul 21.  Review. **PMID:** 28738223  **Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification.** Hammond RW, Zhang S. J Virol Methods. 2016 Oct;236:62-67. doi: 10.1016/j.jviromet.2016.06.013. Epub 2016 Jul 15. **PMID:** 27427473  **Recombinant Shee****p Pox Virus Proteins Elicit Neutralizing Antibodies.** Chervyakova OV, Zaitsev VL, Iskakov BK, Tailakova ET, Strochkov VM, Sultankulova KT, Sandybayev NT, Stanbekova GE, Beisenov DK, Abduraimov YO, Mambetaliyev M, Sansyzbay AR, Kovalskaya NY, Nemchinov LG, Hammond RW. Viruses. 2016 Jun 7;8(6). pii: E159. doi: 10.3390/v8060159. **PMID:** 27338444  **Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China.** Wang J, Zhu D, Tan Y, Zong X, Wei H, Hammond RW, Liu Q. Arch Virol. 2016 Mar;161(3):749-53. doi: 10.1007/s00705-015-2737-z. Epub 2016 Jan 5. **PMID:** 26733294  **Antimicrobial Activity of Bacteriophage Endolysin Produced in Nicotiana benthamiana Plants.** Kovalskaya N, Foster-Frey J, Donovan DM, Bauchan G, Hammond RW. J Microbiol Biotechnol. 2016 Jan;26(1):160-70. doi: 10.4014/jmb.1505.05060. **PMID:** 26403819  **In silico prediction and validation of potential gene targets for pospiviroid-derived small RNAs during tomato infection.** Avina-Padilla K, Martinez de la Vega O, Rivera-Bustamante R, Martinez-Soriano JP, Owens RA, Hammond RW, Vielle-Calzada JP. Gene. 2015 Jun 15;564(2):197-205. doi: 10.1016/j.gene.2015.03.076. Epub 2015 Apr 8. **PMID:** 25862922  **Molecular biology of viroid-host interactions and disease control strategies.** Kovalskaya N, Hammond RW. Plant Sci. 2014 Nov;228:48-60. doi: 10.1016/j.plantsci.2014.05.006. Epub 2014 May 22. Review. **PMID:** 25438785  **Mutations in the alpha-helical region of the amino terminus of the Maize rayado fino virus capsid protein and CP:RNA ratios affect virus-like particle encapsidation of RNAs.** Natilla A, Murphy C, Hammond RW. Virus Res. 2015 Jan 22;196:70-8. doi: 10.1016/j.virusres.2014.07.033. Epub 2014 Aug 5. **PMID:** 25102332  **Application of a modified EDTA-mediated exudation technique and guttation fluid analysis for Potato spindle tuber viroid RNA detection in tomato plants (Solanum lycopersicum).** Kovalskaya N, Owens R, Baker CJ, Deahl K, Hammond RW. J Virol Methods. 2014 Mar;198:75-81. doi: 10.1016/j.jviromet.2013.12.015. Epub 2013 Dec 31. **PMID:** 24388932  **Analysis of the solvent accessibility of cysteine residues on Maize rayado fino virus virus-like particles produced in Nicotiana benthamiana plants and cross-linking of peptides to VLPs.** Natilla A, Hammond RW. J Vis Exp. 2013 Feb 14;(72). pii: 50084. doi: 10.3791/50084. **PMID:** 23439009  **Complete genome sequence of switchgrass mosaic virus, a member of a proposed new species in the genus Marafivirus.** Agindotan BO, Gray ME, Hammond RW, Bradley CA. Arch Virol. 2012 Sep;157(9):1825-30. doi: 10.1007/s00705-012-1354-3. Epub 2012 Jun 4. **PMID:** 22661377  **Maize rayado fino virus virus-like particles expressed in tobacco plants: A new platform for cysteine selective bioconjugation peptide display.** Natilla A, Hammond RW. J Virol Methods. 2011 Dec;178(1-2):209-15. doi: 10.1016/j.jviromet.2011.09.013. Epub 2011 Sep 22. **PMID:** 21963393  **Advances in plant virus evolution: translating evolutionary insights into better disease management.** Acosta-Leal R, Duffy S, Xiong Z, Hammond RW, Elena SF. Phytopathology. 2011 Oct;101(10):1136-48. doi: 10.1094/PHYTO-01-11-0017. **PMID:** 21554186  **Newly Discovered Natural Hosts of Tomato chlorosis virus in Costa Rica.** Solórzano-Morales A, Barboza N, Hernández E, Mora-Umaña F, Ramírez P, Hammond RW. Plant Dis. 2011 Apr;95(4):497. doi: 10.1094/PDIS-11-10-0836. **PMID:** 30743346  **Complete genomic sequence of a Tobacco rattle virus isolate from Michigan-grown potatoes.** Crosslin JM, Hamm PB, Kirk WW, Hammond RW. Arch Virol. 2010 Apr;155(4):621-5. doi: 10.1007/s00705-010-0609-0. Epub 2010 Mar 11. **PMID:** 20221838  **Maize rayado fino virus capsid proteins assemble into virus-like particles in Escherichia coli.** Hammond RW, Hammond J. Virus Res. 2010 Feb;147(2):208-15. doi: 10.1016/j.virusres.2009.11.002. Epub 2009 Nov 13. **PMID:** 19914312  **First Report of Tomato chlorosis virus in Tomato in Costa Rica.** Castro RM, Hernandez E, Mora F, Ramirez P, Hammond RW. Plant Dis. 2009 Sep;93(9):970. doi: 10.1094/PDIS-93-9-0970A. **PMID:** 30754550  **Viroid pathogenicity: one process, many faces.** Owens RA, Hammond RW. Viruses. 2009 Sep;1(2):298-316. doi: 10.3390/v1020298. Epub 2009 Sep 10. **PMID:** 21994551  Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling. **Hammond RW**, Zhao Y. BMC Plant Biol. 2009 Aug 18;9:108. doi: 10.1186/1471-2229-9-108. PMID: 19689802  Retention of the ability to synthesize HIV-1 and HBV antigens in generations of tomato plants transgenic for the TBI-HBS gene. Salyaev RK, Rekoslavskaya NI, Stolbikov AS, **Hammond RW**, Shchelkunov SN. Dokl Biochem Biophys. 2009 Mar-Apr;425:120-3. No abstract available. PMID: 19496338  Plant production of veterinary vaccines and therapeutics. **Hammond RW**, Nemchinov LG. Curr Top Microbiol Immunol. 2009;332:79-102. doi: 10.1007/978-3-540-70868-1_5. Review. PMID: 19401822  Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins. Kovalskaya N, **Hammond RW**. Protein Expr Purif. 2009 Jan;63(1):12-7. doi: 10.1016/j.pep.2008.08.013. Epub 2008 Sep 13. PMID: 18824107  Synthesis of hepatitis B virus surface antigen in tomato plants transgenic for the preS2-S gene. Salyaev K, Rekoslavskaya NI, Stolbikov AS, **Hammond RW**, Shchelkunov SN. Dokl Biochem Biophys. 2007 Sep-Oct;416:290-3. No abstract available. PMID:  Bovine CD14 receptor produced in plants reduces severity of intramammary bacterial infection. Nemchinov LG, Paape MJ, Sohn EJ, Bannerman DD, Zarlenga DS, **Hammond RW**. FASEB J. 2006 Jul;20(9):1345-51. PMID: 16816109  Expression of a bioactive bacteriophage endolysin in Nicotiana benthamiana plants - (Peer Reviewed Journal)  Kovalskaya, N.Y., Foster Frey, J.A., Donovan, D.M., Bauchan, G.R., Hammond, R. 2015. Expression of a bioactive bacteriophage endolysin in Nicotiana benthamiana plants. Journal of Microbiology and Biotechnology. 26:160-170.  Genomic prediction and validation of Pospiviroidae-derived small RNAs and their targets during tomato infection  - (Peer Reviewed Journal)  Avina-Padilla, K., Martinez De La Vega, O., Rivera-Bustamente, R., Martinez-Soriano, J., Owens, R.A., Hammond, R., Vielle-Calzada, J. 2015. Genomic prediction and validation of Pospiviroidae-derived small RNAs and their targets during tomato infection. Gene. 564:197-205.  Survey of diagnostic tools for detection of viroids and impacts of test results on the seed industry- (Abstract Only)  Incidence of sweet cherry viruses in Shandong Province, China and a case study on multiple infection with five viruses - (Peer Reviewed Journal)  Zong, X., Wang, W., Wei, H., Wang, J., Yan, X., Hammond, R., Liu, Q. 2015. Incidence of sweet cherry viruses in Shandong Province, China and a case study on multiple infection with five viruses. Journal of Plant Pathology. 9:61-68.  Bacteriophage endolysin production in Nicotiana benthamiana plants- (Abstract Only)  Potato spindle tuber viroid detection in phloem exudates and guttation fluid of tomato plants (Solanum lycopersicum) - (Abstract Only)  Characterization of whitefly and whitefly-borne virus populations in tomato- and sweet pepper-growing greenhouses in the Cartago province, Costa Rica- (Peer Reviewed Journal)  Vargas-Asencio, J., Hernandez, E., Barboza, N., Hammond, R., Mora, F., Ramirez, P. 2013. Characterization of whitefly and whitefly-borne virus populations in tomato- and sweet pepper-growing greenhouses in the Cartago province, Costa Rica. Journal of Plant Pathology. 95:627-630.  RNA packaging of MRFV virus-like particles: The interplay between RNA pools and capsid coat protein - (Abstract Only)  Evaluating the silencing suppressor activity of proteins encoded by maize rayado fino virus - (Abstract Only)  Hammond, R. 2013. Evaluating the silencing suppressor activity of proteins encoded by maize rayado fino virus. Meeting Abstract. Phytopathology Vol. 103 (S2), 56. ISSN: 0031-949X.  Epitope presentation system based on cucumber mosaic virus coat protein expressed from a potato virus X-based vector. Natilla A1, Hammond RW, Nemchinov LG.  Partial characterization of Maize rayado fino virus isolates from Ecuador: phylogenetic analysis supports a Central American origin of the virus. Chicas M1, Caviedes M, Hammond R, Madriz K, Albertazzi F, Villalobos H, Ramírez P.  Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses. Shchelkunov SN1, Salyaev RK, Pozdnyakov SG, Rekoslavskaya NI, Nesterov AE, Ryzhova TS, Sumtsova VM, Pakova NV, Mishutina UO, Kopytina TV, Hammond RW.  Characterization of apoplast phenolics: In vitro oxidation of acetosyringone results in a rapid and prolonged increase in the redox potential C. JacynBakeraNorton M.MockaBruce D.WhitakerbRose W.HammondaLevNemchinovaDaniel P.RobertscAndrey A.Aver'yanovd  DETECTION OF TOMATO CHLOROSIS VIRUS AND ITS VECTOR TRIALEURODES VAPORARIORUM IN GREENHOUSE-GROWN TOMATO AND SWEET PEPPER IN THE CARTAGO PROVINCE, COSTA RICA *J.A. Vargas-Asencio, E. Hernández, N. Barboza, R. Hammond, F. Mora, P. Ramírez*  Real-time monitoring of the extracellular redox potential of cell suspensions during plant/bacterial interactions Author links open overlay panelC. JacynBakeraNatalia Y.KovalskayaaNorton M.MockaKenneth L.DeahldBruce D.WhitakerbDaniel P.RobertscRose W.HammondaAndrey A.Aver'yanove  Role of gibberellic acid in tomato defence against potato purple top phytoplasma infection Ding W. Wei W. Wu  R.E. Davis  Y. Jiang  I.‐M. Lee  R.W. Hammond  L. Shen  J.‐P. Sheng  Y. Zhao  Potato purple top phytoplasma‐induced disruption of gibberellin homeostasis in tomato plants Ding W. Wu W. Wei  R.E. Davis  I.‐M. Lee  R.W. Hammond  J.P. Sheng  L. Shen  Y. Jiang  Y. Zhao  An internal standard technique for improved quantitative analysis of apoplastic metabolites in tomato leaves C. JacynBakeraNatalia Y.KovalskayaeNorton M.MockaRobert A.OwensaKenneth L.DeahldBruce D.WhitakerbDaniel P.RobertscRose W.HammondaAndrey A.Aver'yanovf  Bemisia tabaci Biotype Q is present in Costa Rica Jose Andres Guevara-CotoEmail authorNatalia Barboza-VargasEduardo Hernandez-JimenezRosemarie W. HammondPilar Ramirez-Fonseca  High-efficiency and stable genetic transformation of pear (Pyrus communis L.) leaf segments and regeneration of transgenic plants Qingrong Sun, Yan Zhao, Hongyan Sun, Rose W. Hammond,Robert E. Davis, Li Xin  LIMITED GEOGRAPHIC DISTRIBUTION OF BEET PSEUDO-YELLOWS VIRUS IN COSTA RICAN CUCURBITS *Ramirez, E. Hernandez, F. Mora, R. Abraitis, R.W. Hammond*  Nuclear targeting by fragmentation of the Potato spindle tuber viroid genome AstaAbraitieneabYanZhaobRosemarieHammondb  OBPC Symposium: Maize 2004 & beyond—Plant virus-based vectors in agriculture and biotechnology Rosemarie W. Hammond  Development of a candidate vaccine for Newcastle disease virus by epitope display in the Cucumber mosaic virus capsid protein. Zhao Y1, Hammond RW.  Study of immunogenic properties of the candidate edible vaccine against human immunodeficiency and hepatitis B viruses based on transgenic tomato fruits. Shchelkunov SN1, Salyaev RK, Rekoslavskaya NI, Pozdnyakov SG, Nesterov AE, Sumtsova VM, Pakova NV, Mishutina UO, Kopytina TV, Hammond R.  The complete nucleotide sequence, genome organization, and specific detection of Beet mosaic virus. Nemchinov LG1, Hammond J, Jordan R, Hammond RW.  Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii. Zhao Y1, Wang H, Hammond RW, Jomantiene R, Liu Q, Lin S, Roe BA, Davis RE.  IDENTIFICATION AND PARTIAL CHARACTERIZATION OF PRUNUS NECROTIC RINGSPOT VIRUS ON STONE FRUITS IN JORDAN *Salem, A. Mansour, A. Al-Musa, A. Al-Nsour, R. Hammond*  Cell division gene cluster in Spiroplasma kunkelii: functional characterization of ftsZ and the first report of ftsA in mollicutes. Zhao Y1, Hammond RW, Lee IM, Roe BA, Lin S, Davis RE.  The complete nucleotide sequence of isolate BYMV-GDD of Bean yellow mosaic virus, and comparison to other potyviruses. Hammond J1, Hammond RW.  Genetic variability of genomic RNA 2 of four tobacco rattle tobravirus isolates from potato fields in the Northwestern United States. Crosslin JM1, Thomas PE, Hammond RW.  Gene content and organization of an 85-kb DNA segment from the genome of the phytopathogenic mollicute Spiroplasma kunkelii. Zhao Y1, Hammond RW, Jomantiene R, Dally EL, Lee IM, Jia H, Wu H, Lin S, Zhang P, Kenton S, Najar FZ, Hua A, Roe BA, Fletcher J, Davis RE.  Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features. Hammond RW1.  Use of a vector based on Potato virus X in a whole plant assay to demonstrate nuclear targeting of Potato spindle tuber viroid. Zhao Y1, Owens RA, Hammond RW.  Molecular characterization of the genome of Maize rayado fino virus, the type member of the genus Marafivirus. Hammond RW1, Ramirez P.  Development and evaluation of a complementation-dependent gene delivery system based on cucumber mosaic virus. Zhao Y1, Hammond J, Tousignant ME, Hammond RW.  Characterization of a tomato protein kinase gene induced by infection by Potato spindle tuber viroid. Hammond RW1, Zhao Y.  Both point mutation and RNA recombination contribute to the sequence diversity of citrus viroid III. Owens RA1, Yang G, Gundersen-Rindal D, Hammond RW, Candresse T, Bar-Joseph M.  Differentiation of closely related but biologically distinct cherry isolates of Prunus necrotic ringspot virus by polymerase chain reaction. Hammond RW1, Crosslin JM, Pasini R, Howell WE, Mink GI.  Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized protoplasts Young‐Min Woo , Asuka Itaya, Robert A. Owens, Li Tang, Rosemarie W. Hammond , Huei‐Chi Chou , Michael M. C. Lai , Biao Ding  A cRNA Probe Detects Prunus Necrotic Ringspot Virus in Three Peach Cultivars after Micrografting and in Peach Shoots following Long-term Culture at 4 °C Heuss , Q. Liu , F.A. Hammerschlag and R.W. Hammond  AGROINFECTION OF PEAR AND APPLE WITH DAPPLE APPLE VIROID RESULTS IN SYSTEMIC INFECTION S.F. Zhu, A. Hadidi, R.W. Hammond  STRAIN-SPECIFIC POLYMERASE CHAIN REACTION ASSAYS FOR DISCRIMINATION OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES R.W. Hammond, J.M. Crosslin, W.E. Howell, G.I. Mink  TRANSMISSION OF PRUNUS NECROTIC RINGSPOT VIRUS TO THREE PEACH CULTIVARS AS MEASURED BY RNA HYBRIDIZATION FOLLOWING IN VITRO MICROGRAFTING Heuss, Q. Liu, F.A. Hammerschlag, R.W. Hammond  Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates. Rosemarie W. Hammond, James Mitchell Crosslin  Evaluation of Maize Germ Plasm for Resistance to Maize Rayado Fino Virus Pedro I. Bustamante, Rosemarie Hammond, and Pilar Ramirez  Variability of geographically distinct isolates of maize rayado fino virus in Latin America. Hammond RW, Kogel R, Ramirez P.  Cell-to-cell movement of potato spindle tuber viroid. Ding B, Kwon MO, Hammond R, Owens R.  Occurrence of citrus viroids in Costa Rica. Villalobos W, Rivera C, Hammond RW.  Destabilization of potato spindle tuber viroid by mutations in the left terminal loop. Hu Y, Feldstein PA, Hammond J, Hammond RW, Bottino PJ, Owens RA.  RNA structural features responsible for potato spindle tuber viroid pathogenicity. Owens RA, Steger G, Hu Y, Fels A, Hammond RW, Riesner D.  Incidence and geographic distribution of maize rayado fino virus (MRFV) in Latin America Kogel, R., Hammond, R.W., Ramirez, P.  Monitoring Prunus Necrotic Ringspot Virus Infection by Hybridization with a cRNA Probe after in vitro Micrografting Kathleen Heuss-LaRosa , Rosemarie Hammond , James M. Crosslin , Christine Hazel' and Freddi A. Hammerschlag  The complete nucleotide sequence of RNA 3 of a peach isolate of Prunus necrotic ringspot virus. Hammond RW, Crosslin JM.  Agrobacterium-mediated inoculation of PSTVd cDNAs onto tomato reveals the biological effect of apparently lethal mutations. Hammond RW.  Use of Mycoplasmalike Organism (MLO) Group-Specific Oligonucleotide Primers for Nested-PCR Assays to Detect Mixed-MLO Infections in a Single Host Plant. Ing-Ming Lee, Dawn E. Gundersen, Rosemarie W. Hammond, Robert Edgar Davis  Chemiluminescent detection of potato and pome fruit viroids by digoxigenin-labeled dot blot and tissue blot hybridization. Podleckis EV, Hammond RW, Hurtt SS, Hadidi A . Mechanism of viroid pathogenesis: differential activation of the interferon-induced, double-stranded RNA-activated, M(r) 68,000 protein kinase by viroid strains of varying pathogenicity. Diener TO, Hammond RW, Black T, Katze MG.  Viroids: the smallest and simplest agents of infectious disease. How do they make plants sick? Diener TO, Owens RA, Hammond RW.  Identification of multiple structural domains regulating viroid pathogenicity. T Sano, T Candresse, R W Hammond, T O Diener, and R A Owens  Analysis of the virulence modulating region of potato spindle tuber viroid (PSTVd) by site-directed mutagenesis Rosemarie W.Hammond  Indian bunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid. Mishra MD, Hammond RW, Owens RA, Smith DR, Diener TO.  Nucleotide sequence and proposed secondary structure of Columnea latent viroid: a natural mosaic of viroid sequences. R Hammond, D R Smith, and T O Diener  Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. Hammond J, Hammond RW.  Infectivity of chimeric viroid transcripts reveals the presence of alternative processing sites in potato spindle tuber viroid Rosemarie W.Hammond, T.O.Diener, Robert A.Owens  Cloned riboprobe for detection of a mycoplasmalike organism. Lee IM, Davis RE, Hammond R, Kirkpatrick B.  Mutational analysis of potato spindle tuber viroid reveals complex relationships between structure and infectivity. Hammond RW, Owens RA.  Site-specific mutagenesis of potato spindle tuber viroid cDNA: Alterations within premelting region 2 that abolish infectivity Robert A. OwensRosemarie W. HammondRichard C. GardnerMichael C. KieferSusan M. ThompsonDean E. Cress


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