# Patent

## Title
Restriction endonuclease enhanced polymorphic sequence detection

## Abstract

Provided is an improved method for the detection of specific polymorphic alleles in a mixed DNA population. The method comprises enriching the relative percentage of a given polymorphic allele that is exponentially amplifiable by PCR. Also provided are methods for selectively enriching target nucleic acid, for example, fetal nucleic acid in a maternal sample. In the case of detecting fetal nucleic acid in a maternal sample, a restriction enzyme is introduced that can discriminate between the alleles of a polymorphic site. Preferably, the maternal allele is digested and nucleic acid comprising the paternal allele is relatively enriched.

## Background
RELATED PATENT APPLICATIONS
This patent application is a national stage of international patent application number PCT/US2008/058317, filed on Mar. 26, 2008, which claims the benefit of U.S. provisional patent application No. 60/908,167, filed on Mar. 26, 2007, entitled “RESTRICTION ENDONUCLEASE ENHANCED POLYMORPHIC SEQUENCE DETECTION.” The entire content of each of these patent applications hereby is incorporated by reference herein, including all text, drawings and tables, in jurisdictions providing for such incorporation.


SEQUENCE LISTING
The instant application contains a Sequence Listing which has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Dec. 7, 2009, is named SEQ68US.txt, and is 72,183 bytes in size.
FIELD OF THE INVENTION
Provided herein are methods for detecting specific alleles in a mixed nucleic acid sample. The methods can be used to detect the presence or absence of fetal nucleic acid in a maternal sample.
BACKGROUND
The analysis of circulating nucleic acids has revealed applications in the non-invasive diagnosis, monitoring, and prognostication of many clinical conditions. For example, for prenatal applications, circulating fetal-specific sequences have been detected and constitute a fraction of the total DNA in maternal plasma. The diagnostic reliability of circulating DNA analysis depends on the fractional concentration of the targeted sequence, the analytical sensitivity, and the specificity. The robust discrimination of sequence differences (e.g., single-nucleotide polymorphisms, or SNPs) between circulating DNA species is technically challenging and demands the adoption of highly sensitive and specific analytical methods.
Current techniques to detect sequence differences in a DNA sample include allele-specific PCR, restriction digest and Southern blot hybridization, restriction endonuclease-mediated selective-PCR (REMS-PCR), and competitive PCR methods involving the use of fluorescent detection probes. The currently available techniques present several disadvantages. For allele-specific PCR, it is often difficult to design assays with a high degree of allele specificity (Nasis et al. Clin Chem. 2004 April; 50(4):694-701). Restriction digest/Southern blot methods require higher amounts of DNA template than the method provided herein, and lack the sensitivity to detect polymorphic sequences comprising a low relative proportion of total DNA. Restriction endonuclease-mediated selective-PCR (REMS-PCR) has the drawback of requiring a thermostable restriction enzyme that cleaves the wild-type allele. REMS-PCR is described in U.S. Pat. No. 6,261,768, which is hereby incorporated by reference. Use of the technique may not always be possible, and this requirement limits the general utility of the REMS-PCR approach. Competitive PCR lacks the sensitivity to detect polymorphic sequences comprising a low relative proportion (<5%) of total DNA. Competitive PCR with allele-specific fluorescent probes lacks the ability to multiplex assays higher than 2-3 assays in a single tube format.
In addition, similar methods utilizing methylation differences between DNA species (for example, US Patent Application Publication No. 20070059707, entitled, “Methods for prenatal diagnosis of chromosomal abnormalities”, which is hereby incorporated by reference) are not effective at low copy numbers of genomic DNA.
SUMMARY
The invention in part provides sequence-specific cleavage of nucleic acid to selectively enrich for a particular target nucleic acid. Polymorphic loci are chosen such that only one allele at the polymorphic locus is cleaved by a given cleavage agent, such as a restriction endonuclease. Oligonucleotide primer pairs designed to flank the polymorphism allow amplification of the polymorphic region, or amplicon, by amplification (e.g., PCR). Prior to or during amplification, nucleic acid samples are incubated with the given restriction endonuclease. In a preferred embodiment, the cleavage agent is introduced prior to amplification. This embodiment results in cleavage of the polymorphic allele or sequence comprising the polymorphic allele that is recognized by the restriction endonuclease, if this allele is present. Cleavage of any template nucleic acid within the amplicon sequence (i.e., between primer pairs) prevents PCR amplification of this template. Therefore, if only one allele of a polymorphism is recognized by the cleavage agent and the corresponding nucleic acid sequence is cleaved by the restriction endonuclease, the relative percentage of the amplifiable alternate polymorphic allele is increased in a manner dependent on the efficiency and specificity of the restriction endonuclease activity. After amplification, the amplified polymorphic alleles can be genotyped or otherwise detected or discriminated by any method known in the art (e.g., using Sequenom's MassARRAY® technology or by RT-PCR).
In one embodiment, the invention in part provides a method for detecting the presence or absence of a target allele at a polymorphic locus in a sample, wherein the sample contains nucleic acid, which comprises: cleaving a nucleic acid comprising a non-target allele at or near the polymorphic locus with a cleavage agent that recognizes and cleaves a non-target allele, but not the target allele; amplifying uncleaved nucleic acid but not cleaved nucleic acid; and analyzing the amplification products from the previous step to determine the presence or absence of the target allele. In a related embodiment, the method also comprises first obtaining a sample suspected of comprising nucleic acid with target and non-target alleles. In a preferred embodiment, the method is used to distinguish between two individuals, for example, between a mother and a fetus, wherein the sample comprises both maternal and fetal nucleic acid. Optionally, the method may be used to quantify the target nucleic acid relative to the non-target nucleic acid.
The invention in part provides methods for enriching for target nucleic acid, comprising cleaving nucleic acid comprising a non-target allele with a restriction endonuclease that recognizes the nucleic acid comprising the non-target allele but not the target allele; and amplifying uncleaved nucleic acid but not cleaved nucleic acid, wherein the uncleaved, amplified nucleic acid represents enriched target nucleic acid relative to non-target nucleic acid. In one embodiment, the methods may be utilized to determine the presence or absence of target nucleic acid in a background of non-target nucleic acid. In a related embodiment, the amplification products can be analyzed to diagnose, monitor or prognose a clinical condition. Likewise, the amplification products can be analyzed to assist in the diagnosis, prognosis or monitoring of a clinical condition or chromosomal abnormality. Nucleic acid may be selected such that it comprises an allele having a polymorphic site that is susceptible to selective digestion by a cleavage agent, for example.
The methods are useful for analyzing nucleic acid including, but not limited to, DNA, RNA, mRNA, oligonucleosomal, mitochondrial, epigenetically-modified, single-stranded, double-stranded, circular, plasmid, cosmid, yeast artificial chromosomes, artificial or man-made DNA, including unique DNA sequences, and DNA that has been reverse transcribed from an RNA sample, such as cDNA, and combinations thereof. In one embodiment, the method is used to detect or selectively enrich RNA.
The nucleic acid may also be characterized as target nucleic acid or non-target nucleic acid, wherein target nucleic comprises the target allele and non-target nucleic acid comprises the non-target allele. In one embodiment, the target nucleic acid comprises the paternal allele and the non-target nucleic acid comprises the maternal allele. In certain embodiments, the nucleic acid is cell-free nucleic acid or partially cell-free nucleic acid. In certain embodiments, the target nucleic acid is apoptotic or partially apoptotic. In certain embodiments, the target nucleic acid is less than 2000, 1200, 1100, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80, 70, 60, 50, 40 or less base pairs in length.
The methods may be used to detect target nucleic acid in a biological sample. Preferably, the biological sample is from an animal, preferably a human. In a related embodiment, the biological sample is selected from the group of whole blood, serum, plasma, umbilical cord blood, chorionic villi, amniotic fluid, cerbrospinal fluid, spinal fluid, lavage fluid, biopsy sample, urine, feces, sputum, saliva, nasal mucous, prostate fluid, semen, lymphatic fluid, bile, tears, sweat, breast milk, breast fluid, embryonic cells and fetal cells, and mixture thereof. In one embodiment, the sample is from a crime scene (e.g., used for forensic analysis). In certain embodiments, the biological sample is obtained through non-invasive means, for example, a blood draw from a pregnant female. In another preferred embodiment, the biological sample is cell-free. In certain embodiments, the sample is a previously isolated sample of nucleic acids.
In one embodiment, the invention in part provides a method for detecting the presence or absence of fetal nucleic acid in a maternal sample, wherein the sample contains nucleic acid, which comprises: cleaving nucleic acid comprising a maternal allele with a restriction endonuclease that recognizes and cleaves the nucleic acid comprising the maternal allele but not the paternal allele; amplifying uncleaved nucleic acid but not cleaved nucleic acid; and analyzing the amplification products from the previous step to determine the presence or absence of fetal nucleic acid. In certain embodiments, the sample comprises a mixture of nucleic acids. For example, the mixture may comprise nucleic acid from different species or from different individuals. In one embodiment, the sample is from a pregnant female. Samples can be collected from human females at 1-4, 4-8, 8-12, 12-16, 16-20, 20-24, 24-28, 28-32, 32-36, 36-40, or 40-44 weeks of fetal gestation, and preferably between 5-28 weeks of fetal gestation. In certain embodiments, the methods may be used to detect the presence or absence of fetal Y-chromosome nucleic acid, thereby determining the sex of the fetus.
In certain embodiments, the target nucleic acid comprises a paternal allele. In certain embodiments, the mother is homozygous at the polymorphic site and the fetus is heterozygous at the polymorphic site. In the case when the mother is homozygous at the polymorphic site and the fetus is heterozygous at the polymorphic site, the polymorphic site is considered informative (see FIG. 5A for examples of informative and non-informative cases). In a related embodiment, the maternal genotype is determined in conjunction with the methods provided herein. In a related embodiment, the mother is first genotyped (for example, using peripheral blood mononuclear cells (PBMC) from a maternal whole blood sample) to determine the non-target allele that will be recognized and cleaved by the cleavage agent. When the method is used for forensic purposes, the victim may be first genotyped to determine the non-target allele that will be recognized and cleaved by the cleavage agent. Likewise, when used for organ transplant-related applications, the transplant recipient may be first genotyped to determine the non-target allele that will be recognized and cleaved by the cleavage agent.
In some embodiments, the sample contains nucleic acid from two different individuals. Such instances include, but are not limited to, organ transplant recipients, transfusion recipients, and forensic applications.
In certain embodiments, the sample is from an individual suspected of suffering from a disease, and the non-target allele is a wild-type allele that is selectively cleaved in order to enrich for a disease-related point mutation. In a related embodiment, the disease is cancer. The ras proto-oncogenes, K-ras, N-ras, and H-ras, and the p53 tumour suppressor gene are examples of genes which are frequently mutated in human cancers. Specific mutations in these genes leads to activation or increased transforming potential.
The invention in part provides methods useful for detecting rare alleles or low copy number alleles. In one embodiment, the target allele is undetectable by conventional or unmodified genotyping methods if the non-target allele is not selectively cleaved. In a related embodiment, the target allele is not detectable unless it is selectively enriched, for example, by the methods provided herein. In certain embodiments, the target allele concentration (e.g., allele concentration in a sample) is less than 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10% 20%, 25%, 30% relative to the non-target allele concentration. In certain embodiments, the target nucleic acid number is less than 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 50, 55, 60, 70, 80, 90, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900 or 1000. In certain embodiments, the target allele is a mutation, and the non-target allele is the wild-type allele. In a related embodiment, the target allele may be either a somatic or germline mutation. In certain embodiments, another allele or sequence identifier in the same amplicon as the polymorphic locus may be detected. For example, a sequence comprising a target allele may be selectively enriched using the methods provided herein, and another sequence identifier may be detected by any method known in the art.
In some embodiments, there are no other polymorphic loci within the amplicon that may be recognized by the cleavage agent.
In certain embodiments, the method optionally comprises first isolating nucleic acid from the sample. DNA isolation from blood, plasma, or serum of the pregnant mother can be performed using any method known to one skilled in the art. Any standard DNA isolation technique can be used to isolate the fetal DNA and the maternal DNA including, but not limited to, QIAamp DNA Blood Midi Kit supplied by QIAGEN. Other standard methods of DNA isolation are described, for example, in (Sambrook et al., Molecular Biology: A laboratory Approach, Cold Spring Harbor, N.Y. 1989; Ausubel, et al., Current protocols in Molecular Biology, Greene Publishing, Y, 1995). A preferred method for isolation of plasma DNA is described in Chiu et al., 2001, Clin. Chem. 47: 1607-1613, which is herein incorporated by reference in its entirety. Other suitable methods are provided in Example 2 of PCT International Application Publication Number 2007/028155, filed on Sep. 1, 2006.
Methods described herein allow for the use of any cleavage agent capable of distinguishing between two different sequences, and cleaving within the amplicon sequence, thereby preventing amplification of the cleaved sequence. The difference between the sequences may be the result of different alleles at one or more polymorphic sites within the sequence. In another example, the difference between the sequences may be the result of two homologous sequences, for example, between paralogous genes or between highly homologous genes such as the RhD gene, which encodes the D polypeptide, and the RHCE gene, which encodes the CcEe polypeptide. An example of a cleavage agent is a restriction enzyme, also referred to as a restriction endonuclease. Multiple restriction endonucleases (available from various vendors) may be selected that correspond to appropriate sequence differences. In a preferred embodiment, the restriction enzyme is HpyCH4V. In another preferred embodiment restriction enzyme Tsp5091. In certain embodiments, a step is added to end the cleaving activity of the cleavage agent, for example, by introducing a protease and/or high temperature prior to amplification.
The restriction endonuclease may be added prior to or during amplification. In one embodiment, the restriction endonuclease is added less than 5 minutes, 5 minutes, 10 minutes, 20 minutes, 30 minutes, 40 minutes, 50 minutes, 60 minutes, 90 minutes or 120 or more minutes before amplification. Incubation time may be shortened if additional units of restriction enzyme are added to the reaction. Conversely, longer incubation times are often used to allow a reaction to proceed to completion with fewer units of enzyme. This is contingent on how long a particular enzyme can survive (maintain activity) in a reaction. Some enzymes survive for long periods (>16 hours) while others survive only an hour or less in a reaction. In certain embodiments, the restriction enzyme digests greater than 40%, 50%, 60%, 70%, 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% of the non-target nucleic acid. However, if digestion of non-target nucleic acid of less than 40% allows for useful enrichment of target nucleic acid, it is within the scope of the invention. In certain embodiments, the restriction enzyme digests substantially all of the non-target nucleic acid. In certain embodiments, when the restriction endonuclease is added during amplification, the restriction endonuclease is a thermostable restriction endonuclease which retains activity during thermocycling. Examples of thermostable endonucleases include, but are not limited to, Bst NI, Bsl I, Tru 9I and Tsp 509 I. In certain embodiments, the cleavage agent is not thermostable (non-thermostable), especially when it is preferred that the digestion occurs prior to the amplification step. In a preferred embodiment, the cleavage agent is not thermostable and digestion of the non-target nucleic acid occurs prior to the amplification step. In certain embodiments, a step is introduced to prevent or to reduce digestion during the amplification step.
In one embodiment, the units of restriction enzyme added to the sample is 0.10, 0.25, 0.50, 0.75, 1.0, 2.0 or more. Note that DNA substrates are digested at varying rates, therefore, the actual number of units required for a complete or substantially complete digestion may vary from assay to assay.
In certain embodiments, only one restriction endonuclease is used to digest one or more non-target alleles in a single reaction. For example, a multiplexed assay may be designed wherein a single restriction endonuclease performs multiple (e.g., greater than 5, 10, 15, 20, 25, 50, 100) digestions across the genome. In certain embodiments, more than one restriction endonuclease (e.g., greater than or equal to 2, 3, 4, 5, 6, 7, 8, 9, 10) is used to make multiple (e.g., greater than 5, 10, 15, 20, 25, 50, 100) digestions across the genome.
Amplification may be performed after or during the cleavage of the non-target allele, and prior to the detection of the target allele. In a preferred embodiment, amplification is performed after cleavage of the non-target allele. Amplification can be performed by any method known in the art, including but not limited to polymerase chain reaction (PCR), ligase chain reaction, transcription-based amplification, restriction amplification, or rolling circle amplification, using primers that anneal to the selected fetal DNA regions. In the case that amplification requires thermocycling, cycling greater than 90° C. may be performed such that the cleavage agent is inactivated. Oligonucleotide primers are selected such that they anneal to the sequence to be amplified. In one embodiment, primers are designed such that one or both primers of the primer pair contain sequence recognizable by one or more restriction endonucleases.
Following amplification, the relative enrichment of the target allele in the sample allows accurate detection of allele frequencies using practically any method of nucleic acid detection known in the art. For example, any of the following methods may be used, including, but not limited to, primer extension or microsequencing methods, ligase sequence determination methods, mismatch sequence determination methods, microarray sequence determination methods, restriction fragment length polymorphism (RFLP) procedures, PCR-based assays (e.g., TAQMAN® PCR System (Applied Biosystems)), nucleotide sequencing methods, hybridization methods, conventional dot blot analyses, single strand conformational polymorphism analysis (SSCP), denaturing gradient gel electrophoresis (DGGE), heteroduplex analysis, mismatch cleavage detection, detection by mass spectrometry, real time-PCR and pyrosequencing.
The methods may also be multiplexed at high levels in a single reaction. For example, one or more alleles can be detected simultaneously. Multiplexing embodiments are particularly important when the genotype at a polymorphic locus is not known. In some instances, for example when the mother is heterozygous at the polymorphic locus, the assay may not be informative. See FIG. 5A, which further describes the use of polymorphic variants to detect fetal nucleic acid from a maternal sample. In one embodiment, greater than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100, 200, 300, 500 or more target alleles are assayed, wherein not every target allele is informative. In certain embodiments, the genotype at the polymorphic locus is known. In some embodiments, greater than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 50 or more informative target alleles are assayed. The invention also in part includes combinations of different multiplex schemes provided herein.
In certain embodiments, the invention in part provides a method for quantifying a target allele at a polymorphic locus in a sample, wherein the sample contains nucleic acid, that comprises: digesting nucleic acid containing a maternal allele at the polymorphic locus with an enzyme, such as a restriction endonuclease, that selectively digests the maternal allele, wherein the selective digestion yields a DNA sample enriched for fetal DNA; determining the maternal or paternal allele frequency using polymorphic markers within the amplicon, and comparing the paternal or maternal allele frequency to a control DNA sample. In one embodiment, a difference in allele frequency is indicative of a chromosomal abnormality. In certain embodiments, the control DNA sample is a competitor oligonucleotide that is introduced to the assay in known quantities.
In certain embodiments, the present invention in part provides a kit for detecting the presence or absence of target nucleic acid. One component of the kit is primers for amplifying the region of interest. Another component of the kit comprises probes for discriminating between the different alleles of each nucleic acid species.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 is the HpyCH4V digest, which shows allele peak area ratios in a DNA mixture series. Peak area ratio is determined by dividing the calculated peak area of the SNP allele not recognized by HpyCH4V (i.e., target allele) by the total peak area of both SNP alleles present in the mass spectrum.
FIG. 2 is the NlaIII digest, which shows allele peak area ratios in a DNA mixture series. Peak area ratio is determined by dividing the calculated peak area of the SNP allele not recognized by NlaIII (i.e., target allele) by the total peak area of both SNP alleles present in the mass spectrum.
FIG. 3 is the HpyCH4V screenshots of 2% heterozygous DNA mixture. Note the appearance of the ‘A’ and ‘T’ alleles after HpyCH4V digestion of the DNA samples for rs4329520 and rs4658481, respectively.
FIG. 4 is the NlaIII screenshots of 2% heterozygous DNA mixture. Note the appearance of the ‘T’ and ‘A’ alleles after NlaIII digestion of the DNA samples for rs2050927 and rs4329520, respectively.
FIG. 5A shows the use of single nucleotide polymorphisms (SNP's) Fetal Identifiers to confirm the presence of fetal DNA by paternally-inherited alleles. FIG. 5B shows representative mass spectra demonstrating the correlation between fetal DNA amounts estimated from AMG XY and from Fetal Identifier assays. The results were generated using the AMG primers provided in FIG. 9.
FIG. 6 depicts typical performance results for a qualified fetal identifier. Here the ability of the SNP assay to estimate the quantity of fetal DNA in the background of maternal DNA was verified for a total of 1700 copies and a total of 170 copies using genomic DNA mixtures. Note that the standard deviation of the estimate of fetal DNA increases due to the significant influence of the sampling error at low copy numbers.
FIG. 7 shows the performance of multiplexed SNP assays (21 assays total) for detection of paternally-inherited alleles in a model system.
FIGS. 8A-8C provide the location design of the AMG primers. The amplification primers are underlined once and the extend primers are underlined twice. In addition, competitor sequences are provided. Competitor sequences may be used for quantitative methods. FIG. 8C includes a Results Table that shows the different masses generated by each of the AMG and SRY assays, which may be used to interpret the results from the assays. FIG. 8A discloses SEQ ID NOS 275-283, respectively, in order of appearance. FIG. 8B discloses SEQ ID NOS 284-290, respectively, in order of appearance. FIG. 8C discloses SEQ ID NOS 291-294, 279, 295-297, 279, 296, 298-301, respectively in order of appearance.
FIG. 9 provides the location design of the albumin (ALB) primers. The amplification primers are highlighted and the extend primer is underlined twice. Where the PCR primers are provided alone, the sequence-specific portion of the primer is underlined, and the multiplex tag is not underlined. In addition, competitor sequences are provided. Competitor sequences may be used for quantitative methods FIG. 9 discloses SEQ ID NOS 302 and 302-307, respectively, in order of appearance.



DETAILED DESCRIPTION
It has been determined in the fields of biology and diagnostics that certain nucleic acids are present at very low concentrations in humans. In particular, fetal DNA has been found to exist in maternal plasma (Lo et al. Lancet. 1997 Aug. 16; 350(9076):485-7). This discovery has facilitated the development of non-invasive prenatal diagnostic approaches based simply on the analysis of a maternal blood sample (Lo et al. Am J Hum Genet. 1998 April; 62(4):768-75). The non-invasive nature of maternal plasma-based approaches represents a major advantage over conventional methods of prenatal diagnosis, such as amniocentesis and chorionic villus sampling, which are associated with a small but finite risk of fetal loss. However, a technical challenge experienced by many workers in the field relates to the ability to discriminate the relatively small amount of fetal DNA from the coexisting background of maternal DNA in maternal plasma. During pregnancy, fetal DNA amounts to approximately 3-6% of the total DNA in maternal plasma. Hence, the diagnostic reliability of fetal DNA analysis in maternal plasma generally has depended on the accurate detection of fetal-specific markers.
Methods described herein solve this problem by enriching, relatively, the amount of low copy number nucleic acid before detecting or quantifying the alleles present in the sample. In the case of prenatal diagnostics, the use of restriction endonuclease enhanced polymorphic sequence detection allows for the selective, sensitive detection of fetal nucleic acid from maternal samples. The fetal DNA in the maternal plasma sample is selectively enriched before detecting the alleles present in the maternal sample. To enrich for fetal DNA present in plasma of the mother to allow accurate detection of fetal alleles present in the sample, the methods described herein allow for the cleavage of maternal nucleic acid or nucleic acid of maternal origin. Thus, the maternal DNA can be substantially reduced, masked, or destroyed completely, and the sample is left with DNA enriched for DNA of fetal origin. The selective reduction of maternal DNA can be performed using one or more enzymes, such as restriction endonucleases, which selectively digest nucleic acids which comprise maternal alleles.
DEFINITIONS
The term “sample” as used herein includes a specimen or culture (e.g., microbiological cultures) that includes nucleic acids. The term “sample” is also meant to include both biological and environmental samples. A sample may include a specimen of synthetic origin. Biological samples include whole blood, serum, plasma, umbilical cord blood, chorionic villi, amniotic fluid, cerbrospinal fluid, spinal fluid, lavage fluid (e.g., bronchoalveolar, gastric, peritoneal, ductal, ear, athroscopic), biopsy sample, urine, feces, sputum, saliva, nasal mucous, prostate fluid, semen, lymphatic fluid, bile, tears, sweat, breast milk, breast fluid, embryonic cells and fetal cells. The biological sample can be maternal blood, including maternal plasma or serum. In some circumstances, the biological sample is acellular. In other circumstances, the biological sample does contain cellular elements or cellular remnants in maternal blood.
In a preferred embodiment, the sample comprises a mixture of nucleic acids. For example, the mixture may comprise nucleic acid from different species or from different individuals. In one embodiment, the sample is from a pregnant female or a female suspected of being pregnant. In a related embodiment, the sample is procured through non-invasive means (e.g., a blood draw). In certain embodiments the sample is from any animal, including but not limited, human, non-human, mammal, reptile, cattle, cat, dog, goat, swine, pig, monkey, ape, gorilla, bull, cow, bear, horse, sheep, poultry, mouse, rat, fish, dolphin, whale, and shark, or any animal or organism that may be tested for the presence of target nucleic acid.
In a preferred embodiment, the biological sample is blood, and more preferably plasma. As used herein, the term “blood” encompasses whole blood or any fractions of blood, such as serum and plasma as conventionally defined. Blood plasma refers to the fraction of whole blood resulting from centrifugation of blood treated with anticoagulants. Blood serum refers to the watery portion of fluid remaining after a blood sample has coagulated. Environmental samples include environmental material such as surface matter, soil, water, crime scene samples, and industrial samples, as well as samples obtained from food and dairy processing instruments, apparatus, equipment, utensils, disposable and non-disposable items. These examples are not to be construed as limiting the sample types applicable to the present invention.
The term “non-invasive” as used herein refers a method for collecting a sample that poses minimal risk to an individual (e.g., the mother, fetus, victim, etc.). An example of a non-invasive method is a blood draw; whereas examples of invasive methods include amniocentesis and chorionic villus sampling, both of which constitute a finite risk to the fetus.
The terms “target” or “target nucleic acid” as used herein are intended to mean any molecule whose presence is to be detected or measured or whose function, interactions or properties are to be studied, wherein target nucleic comprises the target allele and non-target nucleic acid comprises the non-target allele. Fetal nucleic acid may comprise both target nucleic acid and non-target nucleic when the fetus is heterozygous at a polymorphic locus. Other examples of target nucleic acid include, but are not limited to, trace nucleic acid, mutated nucleic acid, viral nucleic acid and transplant nucleic acid.
The terms “nucleic acid” and “nucleic acid molecule” may be used interchangeably throughout the disclosure. The terms refer to oligonucleotides, oligos, polynucleotides, deoxyribonucleotide (DNA), genomic DNA, mitochondrial DNA (mtDNA), complementary DNA (cDNA), bacterial DNA, viral DNA, viral RNA, RNA, message RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), siRNA, catalytic RNA, clones, plasmids, M13, P1, cosmid, bacteria artificial chromosome (BAC), yeast artificial chromosome (YAC), amplified nucleic acid, amplicon, PCR product and other types of amplified nucleic acid, RNA/DNA hybrids and polyamide nucleic acids (PNAs), all of which can be in either single- or double-stranded form, and unless otherwise limited, would encompass known analogs of natural nucleotides that can function in a similar manner as naturally occurring nucleotides and combinations and/or mixtures thereof. Thus, the term “nucleotides” refers to both naturally-occurring and modified/non-naturally-occurring nucleotides, including nucleoside tri, di, and monophosphates as well as monophosphate monomers present within polynucleic acid or oligonucleotide. A nucleotide may also be a ribo; 2′-deoxy; 2′,3′-deoxy as well as a vast array of other nucleotide mimics that are well-known in the art. Mimics include chain-terminating nucleotides, such as 3′-O-methyl, halogenated base or sugar substitutions; alternative sugar structures including nonsugar, alkyl ring structures; alternative bases including inosine; deaza-modified; chi, and psi, linker-modified; mass label-modified; phosphodiester modifications or replacements including phosphorothioate, methylphosphonate, boranophosphate, amide, ester, ether; and a basic or complete internucleotide replacements, including cleavage linkages such a photocleavable nitrophenyl moieties.
In the case of RNA, the RNA may be placentally-expressed RNA in maternal plasma. Background maternal RNA may be selectively digested according to the methods provided herein. Also, the method may further comprise the additional step of discriminating the alleles of RNA which involves reverse transcriptase polymerase chain reaction (RT-PCR). In certain embodiments, the fetal RNA may be extracted from maternal body fluids, preferably whole blood, and more preferably plasma or serum using e.g. RNA extraction methods such as, but not limited to, gelatin extraction method; silica, glass bead, or diatom extraction method; guanidinium thiocyanate acid-phenol based extraction methods; guanidinium thiocyanate acid based extraction methods; guanidine-hydrochloride based extraction methods; methods using centrifugation through cesium chloride or similar gradients; phenol-chloroform based extraction methods; and/or other available RNA extraction methods, as are known in the art for use in extraction of intracellular RNA, including commercially available RNA extraction methods, e.g. by using or adapting or modifying the methods of Boom et al. (1990, J. Clin. Microbiol. 28: 495-503); Cheung et al. (1994, J. Clin. Microbiol. 32: 2593-2597); Boom et al. (1991, J. Clin. Microbiol. 29: 1804-1811); Chomczynski and Sacchi (1987, Analytical Biochem. 162: 156-159); Chomczynski, (1993, Biotech. 15: 532-537); Chomczynski and Mackey (1995, Biotechniques 19: 942-945); Chomczynski and Mackey (1995, Anal. Biochem. 225: 163-164); Chirgwin et al. (1979, Biochem. 18: 5294-5299); Fournie et al. (1986 Anal. Biochem. 158: 250-256); and WO97/35589.
The term “amplification reaction” refers to any in vitro means for multiplying the copies of nucleic acid.
“Amplifying” refers to a step of submitting a sample to conditions sufficient to allow for amplification. Components of an amplification reaction may include, but are not limited to, e.g., primers, a polynucleotide template, polymerase, nucleotides, dNTPs and the like. The term “amplifying” typically refers to an “exponential” increase in target nucleic acid. However, “amplifying” as used herein can also refer to linear increases in the numbers of a select target sequence of nucleic acid, but is different than a one-time, single primer extension step.
“Polymerase chain reaction” or “PCR” refers to a method whereby a specific segment or subsequence of a target double-stranded DNA, is amplified in a geometric progression. PCR is well known to those of skill in the art; see, e.g., U.S. Pat. Nos. 4,683,195 and 4,683,202; and PCR Protocols: A Guide to Methods and Applications, Innis et al., eds, 1990.
“Oligonucleotide” as used herein refers to linear oligomers of natural or modified nucleosidic monomers linked by phosphodiester bonds or analogs thereof. Oligonucleotides include deoxyribonucleosides, ribonucleosides, anomeric forms thereof, peptide nucleic acids (PNAs), and the like, capable of specifically binding to a target nucleic acid. Usually monomers are linked by phosphodiester bonds or analogs thereof to form oligonucleotides ranging in size from a few monomeric units, e.g., 3-4, to several tens of monomeric units, e.g., 40-60. Whenever an oligonucleotide is represented by a sequence of letters, such as “ATGCCTG,” it will be understood that the nucleotides are in 5′-3′ order from left to right and that “A” denotes deoxyadenosine, “C” denotes deoxycytidine, “G” denotes deoxyguanosine, “T” denotes deoxythymidine, and “U” denotes the ribonucleoside, uridine, unless otherwise noted. Oligonucleotides often comprise the four natural deoxynucleotides; however, they may also comprise ribonucleosides or non-natural nucleotide analogs. Where an enzyme has specific oligonucleotide or polynucleotide substrate requirements for activity, e.g., single stranded DNA, RNA/DNA duplex, or the like, then selection of appropriate composition for the oligonucleotide or polynucleotide substrates is well within the knowledge of one of ordinary skill.
As used herein “oligonucleotide primer”, or simply “primer”, refers to a polynucleotide sequence that hybridizes to a sequence on a nucleic acid template and facilitates the amplification of the nucleic acid template, or otherwise plays a role in the detection of the nucleic acid molecule. In amplification embodiments, an oligonucleotide primer serves as a point of initiation of nucleic acid synthesis. Primers can be of a variety of lengths and are often less than 50 nucleotides in length, for example 12-25 nucleotides, in length. The length and sequences of primers for use in PCR can be designed based on principles known to those of skill in the art.
The term “template” refers to any nucleic acid molecule that can be used for amplification in the methods described herein. RNA or DNA that is not naturally double stranded can be made into double stranded DNA so as to be used as template DNA. Any double stranded DNA or preparation containing multiple, different double stranded DNA molecules can be used as template DNA to amplify a locus or loci of interest contained in the template DNA.
The term “amplicon” as used herein refers to amplified DNA that has been “copied” once or multiple times, e.g. by polymerase chain reaction. The amplicon sequence falls between the amplification primers.
The term “polymorphic locus” as used herein refers to a nucleic acid region that comprises a polymorphism. The nucleic acid region may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500 or more nucleotides in length.
The term “polymorphism” as used herein refers to an allelic variant. Polymorphisms can include single nucleotide polymorphisms (SNP's) as well as simple sequence length polymorphisms. A polymorphism can be due to one or more nucleotide substitutions at one allele in comparison to another allele or can be due to an insertion or deletion, duplication, inversion and other alterations known to the art. Other polymorphisms include, but are not limited to, restriction fragment length polymorphisms (RFLPs), insertions/deletions, short tandem repeats, such as di-, tri- or tetra-nucleotide repeats (STRs), and the like. As used herein, polymorphism may include epigenetic variants, as long as cleavage by non-epigenetic-specific cleavage agents is used.
The term “allele” as used herein is one of several alternate forms of a gene or non-coding regions of DNA that occupy the same position on a chromosome. The term allele can be used to describe DNA from any organism including but not limited to bacteria, viruses, fungi, protozoa, molds, yeasts, plants, humans, non-humans, animals, and archeabacteria.
Alleles can have the identical sequence or can vary by a single nucleotide or more than one nucleotide. With regard to organisms that have two copies of each chromosome, if both chromosomes have the same allele, the condition is referred to as homozygous. If the alleles at the two chromosomes are different, the condition is referred to as heterozygous. For example, if the locus of interest is SNP X on chromosome 1, and the maternal chromosome contains an adenine at SNP X (A allele) and the paternal chromosome contains a guanine at SNP X (G allele), the individual is heterozygous A/G at SNP X.
As used herein, the term “mutant alleles” may refer to variant alleles that are associated with a disease state, e.g., cancer.
The term “sequence identifier” as used herein refers to any sequence difference that exists between two sequences that can be used to differentiate the sequences. In one embodiment, the sequence identifier does not include methylation differences.
As used herein, the term “genotype” refers to the identity of the alleles or non-homologous variants present in an individual or sample. The term “genotyping a sample” or “genotyping an individual” refers to determining a specific allele or specific nucleotide(s) or polymorphism(s) in a sample or carried by an individual at particular region(s).
The term “selectively” as used herein is not intended to suggest an absolute event, but instead a preferential event. For example, “selectively cleaved” is used to indicate one sequence (for example, the non-target sequence) is preferentially cleaved or digested over another sequence (for example, the target sequence). However, some of the target sequence may also be cleaved due to a lack of specificity with the cleavage agent or other variables introduced during the cleavage process.
The term “cleavage agent” as used herein refers to any means that is capable of differentially cleaving two or more sequences based on a sequence difference that exists between the two or more sequences. The cleavage agent may be an enzyme. The cleavage agent may be natural, synthetic, unmodified or modified. In a preferred embodiment, the cleavage agent is a restriction endonuclease. Restriction endonucleases, alternatively called restriction enzymes, are a class of bacterial enzymes that cut or digest DNA at specific sites. Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site. Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides, and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double-stranded breaks, which, if the site of cleavage is off-center, generates fragments with short single-stranded tails; these can hybridize to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. coli are designated Eco RI and Eco RII. In a preferred embodiment, the restriction enzyme is a type II restriction endonuclease. In another preferred embodiment, the restriction enzyme is non-thermostable.
The term “chromosomal abnormality” as used herein refers to a deviation between the structure of the subject chromosome and a normal homologous chromosome. The term “normal” refers to the predominate karyotype or banding pattern found in healthy individuals of a particular species. A chromosomal abnormality can be numerical or structural, and includes but is not limited to aneuploidy, polyploidy, inversion, a trisomy, a monosomy, duplication, deletion, deletion of a part of a chromosome, addition, addition of a part of chromosome, insertion, a fragment of a chromosome, a region of a chromosome, chromosomal rearrangement, and translocation. A chromosomal abnormality can be correlated with presence of a pathological condition or with a predisposition to develop a pathological condition.
Uses and Advantages Associated with the Methods Described Herein
The invention in part provides nucleic acid-based assays that are particularly useful for non-invasive prenatal testing. The methods provided herein may be used, inter alia, to determine the presence of fetal nucleic acid in a sample, to determine the amount of fetal nucleic acid in a sample, to determine the sex of a fetus, and to enrich for a target nucleic acid sequence. The invention in part may be combined with other prenatal methods, such as those described in U.S. application Ser. No. 12/027,954, filed Feb. 7, 2008; PCT Application No. PCT/US07/69991, filed May 30, 2007; PCT Application No. PCT/US07/071,232, filed Jun. 15, 2007; U.S. Provisional Application No. 61/033,343, filed Mar. 3, 2008; U.S. Provisional Application No. 61/035,711, filed Mar. 11, 2008; or any of the prenatal diagnostic (both invasive and non-invasive) methods disclosed in U.S. Provisional Application No. 60/944,331, filed Jun. 15, 2007, all of which are hereby incorporated by reference.
The invention in part may be used to more accurately detect fetal DNA using high frequency polymorphisms that match the criteria provided herein. These polymorphisms are alternatively called fetal identifiers. The criteria includes one or more of the following:
1) One allele of the SNP is recognized by the cleavage agent;
2) The alternate SNP allele is not recognized by the same cleavage agent;
3) No other sites for the cleavage are found +/−50 base pair of the SNP within the PCR amplicon; and
4) (Optionally) The minor allele frequency is greater than 0.4 (preferably across a range of populations).
Examples of fetal identifiers are set forth in Tables 6, 9, 10 and 11. In one embodiment, the method of detecting the presence or absence of fetal nucleic acid in a sample comprises obtaining or possessing a nucleic acid sample known to be of maternal origin and suspected of comprising fetal nucleic acid; analyzing the nucleic acid sample to determine the maternal genotype at one or more nucleotide polymorphisms selected from the group consisting of the polymorphisms set forth in Tables 6, 9, 10 and 11; and analyzing the nucleic acid sample to determine the fetal genotype of one or more nucleotide polymorphisms selected from the group consisting of the polymorphisms set forth in Tables 6, 9, 10 and 11, wherein a fetal genotype possessing a paternally-inherited allele indicates the presence of fetal nucleic acid, further wherein nucleic acid comprising a maternal allele is digested using the methods provided herein. In a preferred embodiment, one or more of the polymorphisms set forth in Table 6 or 11 are used in conjunction with the methods provided herein. In a related embodiment, the maternal genotypes are first determined from DNA that is substantially free of fetal nucleic acid. For example, in the case when the sample is blood, the maternal genotypes may be determined from the portion of the blood that comprises nucleated maternal cells (e.g., white blood cells). In one embodiment, the DNA that is substantially free of fetal nucleic acid is from peripheral blood mononuclear cells. In certain embodiments, the amount of fetal DNA is determined by comparing the relative amount of paternally-inherited alleles to an internal control (e.g., competitor oligonucleotide).
In Tables 6, 9, 10 and 11, each primer of the amplification primer pair may comprise the entire sequence shown or only the non-underlined sequence, wherein the underlined portion of the primer is a tag sequence (ACGTTGGATG (SEQ ID NO: 1)) for improved multiplexing and the non-underlined portion is a sequence-specific primer sequence. The tag sequence may be any tag sequence known in the art that improves multiplexing. In certain embodiments, the invention in part includes primers that are substantially similar to the primers provided herein, for example, about 90% or more similar, and further wherein the primers are still specific for a given nucleic acid region. For example, one or more bases of a primer sequence may be changed or substituted, for example with an inosine, but the primer still maintains the same specificity and plexing ability. Bases indicated by uppercase text are complementary to the nucleic acid sequence to which the primer hybridizes, and bases indicated by lowercase text are not complementary to the nucleic acid sequence to which the primer hybridizes. Bases indicated in lower case text can be selected to shift or adjust the mass of primers and amplification products.
In particular embodiments, a sequence tag is attached to a plurality of primary and secondary primer pairs provided in Tables 6, 9, 10 and 11. The sequence tag can be attached to either one or both of the primary and secondary primers from each pair. Typically, the sequence tag is attached to the primary and secondary primer of each pair. The sequence tags used herein can range from 5 up to 20, from 5 up to 30, from 5 up to 40, or from 5 up to 50 nucleotides in length, with a sequence tag of 10-mer length being particularly useful in the methods provided herein. The sequence tag need not be the same sequence for each primer pair in the multiplexed amplification reaction, nor the same sequence for a primary and secondary primer within a particular amplification pair. In a particular embodiment, the sequence tag is the same for each primer in the multiplexed amplification reaction. For example, in certain embodiments, the sequence tag is a 10-mer, such as -ACGTTGGATG- (SEQ ID NO: 1), and is attached to the 5′ end of each primary and secondary primer. In particular embodiments of the methods provided herein, only a single primer pair is used to amplify each particular nucleic acid target-region.
In certain embodiments, methods described herein may be used to improve the detection the Y-chromosome in a maternal sample, which may be used to determine the sex of a fetus. The presence or absence of the Y-chromosome in a maternal sample may be determined by performing the SRY assay described in Example 3. The SRY assay is a highly sensitive quantitative internal standard assay that detects trace amounts of the Y-chromosome. In certain embodiments, other polymorphisms located on the Y-chromosome may be assayed according to the methods provided herein.
The presence or absence of the Y-chromosome in a maternal sample may also be determined by performing the AMG assay provided herein. The presence or absence of a target nucleic acid may be determined in combination with other assays, such as an RhD assay, blood type assay or sex test assay. The methods may also be used for other applications, including but not limited to, paternity testing, forensics or quality control assays.
In addition to prenatal applications, the methods find utility in a range of applications, including, but not limited to, detecting rare cancer mutations, detecting transplant rejection and forensics.
In certain embodiments, the total copy number of nucleic acid molecules for the human serum albumin (ALB) gene is determined. Methods for determining the total copy number of nucleic acid present in a sample comprise detecting albumin-specific extension products and comparing the relative amount of the extension products to competitors introduced to the sample. In a related embodiment, the invention in part provides compositions and methods to determine the relative amount of fetal DNA in a sample (e.g., when the sample is plasma from a pregnant woman carrying a male fetus), which comprises annealing one or more albumin gene sequences to the fetal DNA, the primers provided in FIG. 9; performing a primer extension reaction; and analyzing the primer extension products to determine the relative amount of ALB extension products, wherein maternal albumin nucleic acid has been reduced using the methods provided herein. In a related embodiment, the fetal ALB amplicon is first amplified using the amplification primers provided in FIG. 9. The assay is useful to measure how much nucleic acid (e.g., total copy number) is present in a sample or loaded into a particular reaction. The assay may serve as an internal control and a guide to the likelihood of success for a particular PCR reaction. For example, if only 400 copies of ALB are measured then the probability of detecting any fetal DNA may be considered low. In certain embodiments, the competitors provided in FIG. 9 are introduced as an internal standard to determine copy number. In one embodiment, 200, 300, 400, 500, 600, 700, 800 or more competitor molecules are introduced to the assay.
Methods described herein provide a number of advantageous. The methods allow a high sensitivity to detect polymorphic alleles (e.g., fetal identifiers) present at low relative percentages in a DNA mixture and present at low copy number. The methods may also be incorporated into multiplexed assays in a single reaction. The methods are readily implemented, and only add a single additional step to the many current detection methods.
Nucleases
Cleavage methods and procedures for selecting restriction enzymes for cutting nucleic acid at specific sites are well known to the skilled artisan. For example, many suppliers of restriction enzymes provide information on conditions and types of DNA sequences cut by specific restriction enzymes, including New England BioLabs, Pro-Mega Biochems, Boehringer-Mannheim, and the like.
The preparation of nucleic acid to be cleaved should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents, or excessive salts, all of which can interfere with restriction enzyme activity.
Embodiments of the invention can be assembled from multiple restriction endonucleases (available from various vendors) that are chosen to correspond to appropriate polymorphic alleles, as long as the restriction endonuclease selects for one polymorphic allele over another and performs a digestion within the amplicon sequence such that it prevents a subsequent amplification event. In one embodiment, the amplicon is chosen such that it contains a variable nuclease restriction site and sequence identifier, which may or may not be the same as the restriction site. Also, the restriction enzyme need not cleave at the polymorphic site, for example, at the variable nucleotide of a SNP.
Restriction enzymes are traditionally classified into three types on the basis of subunit composition, cleavage position, sequence-specificity and cofactor-requirements. However, amino acid sequencing has uncovered extraordinary variety among restriction enzymes and revealed that at the molecular level there are many more than three different kinds.
Type I enzymes are complex, multisubunit, combination restriction-and-modification enzymes that cut DNA at random far from their recognition sequences. Originally thought to be rare, we now know from the analysis of sequenced genomes that they are common. Type I enzymes are of considerable biochemical interest but they have little practical value since they do not produce discrete restriction fragments or distinct gel-banding patterns.
Type II enzymes cut DNA at defined positions close to or within their recognition sequences. They produce discrete restriction fragments and distinct gel banding patterns, and they are the only class used in the laboratory for DNA analysis and gene cloning. Type II enzymes frequently differ so utterly in amino acid sequence from one another, and indeed from every other known protein, that they likely arose independently in the course of evolution rather than diverging from common ancestors.
The most common type II enzymes are those like HhaI, HindIII and NotI that cleave DNA within their recognition sequences. Enzymes of this kind are the principle ones available commercially. Most recognize DNA sequences that are symmetric because they bind to DNA as homodimers, but a few, (e.g., BbvCI: CCTCAGC) recognize asymmetric DNA sequences because they bind as heterodimers. Some enzymes recognize continuous sequences (e.g., EcoRI: GAATTC) in which the two half-sites of the recognition sequence are adjacent, while others recognize discontinuous sequences (e.g., BglI: GCCNNNNNGGC (SEQ ID NO: 2)) in which the half-sites are separated. Cleavage leaves a 3′′-hydroxyl on one side of each cut and a 5′′-phosphate on the other. They require only magnesium for activity and the corresponding modification enzymes require only S-adenosylmethionine. They tend to be small, with subunits in the 200-350 amino acid range.
The next most common type II enzymes, usually referred to as ‘type IIs” are those like FokI and AlwI that cleave outside of their recognition sequence to one side. These enzymes are intermediate in size, 400-650 amino acids in length, and they recognize sequences that are continuous and asymmetric. They comprise two distinct domains, one for DNA binding, the other for DNA cleavage. They are thought to bind to DNA as monomers for the most part, but to cleave DNA cooperatively, through dimerization of the cleavage domains of adjacent enzyme molecules. For this reason, some type IIs enzymes are much more active on DNA molecules that contain multiple recognition sites. A wide variety of Type IIS restriction enzymes are known and such enzymes have been isolated from bacteria, phage, archeabacteria and viruses of eukaryotic algae and are commercially available (Promega, Madison Wis.; New England Biolabs, Beverly, Mass.). Examples of Type IIS restriction enzymes that may be used with the methods described herein include, but are not limited to enzymes such as those listed in Table IA.









TABLE 1A






Recognition/



Enzyme-Source
Cleavage Site
Supplier







Alw I - Acinetobacter lwoffii
GGATC(4/5)
NE Biolabs


 


Alw26 I - Acinetobacter lwoffi
GTCTC(1/5)
Promega


 


Bbs I - Bacillus laterosporus
GAAGAC(2/6)
NE Biolabs


 


Bbv I - Bacillus brevis
GCAGC(8/12)
NE Biolabs


 


BceA I - Bacillus cereus 1315
IACGGC(12/14)
NE Biolabs


 


Bmr I - Bacillus megaterium
CTGGG(5/4)
NE Biolabs


 


Bsa I - Bacillus
GGTCTC(1/5)
NE Biolabs


stearothermophilus 6-55




 


Bst71 I - Bacillus
GCAGC(8/12)
Promega


stearothermophilus 71




 


BsmA I - Bacillus
GTCTC(1/5)
NE Biolabs


stearothermophilus A664




 


BsmB I - Bacillus
CGTCTC(1/5)
NE Biolabs


stearothermophilus B61




 


BsmF I - Bacillus
GGGAC(10/14)
NE Biolabs


stearothermophilus F




 


BspM I - Bacillus species M
ACCTGC(4/8)
NE Biolabs


 


Ear I - Enterobacter aerogenes
CTCTTC(1/4)
NE Biolabs


 


Fau I - Flavobacterium aquatile
CCCGC(4/6)
NE Biolabs


 


Fok I - Flavobacterium
GGATG(9/13)
NE Biolabs


okeonokoites




 


Hga I - Haemophilus gallinarum
GACGC(5/10)
NE Biolabs


 


Ple I - Pseudomonas lemoignei
GAGTC(4/5)
NE Biolabs


 


Sap I - Saccharopolyspora species
GCTCTTC(1/4)
NE Biolabs


 


SfaN I - Streptococcus faecalis
GCATC(5/9)
NE Biolabs


ND547




 


Sth132 I - Streptococcus
CCCG(4/8)
No commercial


thermophilus ST132

supplier (Gene 195:




201-206 (1997))









The third major kind of type II enzyme, more properly referred to as “type IV” are large, combination restriction-and-modification enzymes, 850-1250 amino acids in length, in which the two enzymatic activities reside in the same protein chain. These enzymes cleave outside of their recognition sequences; those that recognize continuous sequences (e.g., Eco57I: CTGAAG) cleave on just one side; those that recognize discontinuous sequences (e.g., BcgI: CGANNNNNNTGC (SEQ ID NO: 3)) cleave on both sides releasing a small fragment containing the recognition sequence. The amino acid sequences of these enzymes are varied but their organization are consistent. They comprise an N-terminal DNA-cleavage domain joined to a DNA-modification domain and one or two DNA sequence-specificity domains forming the C-terminus, or present as a separate subunit. When these enzymes bind to their substrates, they switch into either restriction mode to cleave the DNA, or modification mode to methylate it.
As discussed above, the length of restriction recognition sites varies. For example, the enzymes EcoRI, SacI and SstI each recognize a 6 base-pair (bp) sequence of DNA, whereas NotI recognizes a sequence 8 bp in length, and the recognition site for Sau3AI is only 4 bp in length. Length of the recognition sequence dictates how frequently the enzyme will cut in a random sequence of DNA. Enzymes with a 6 bp recognition site will cut, on average, every 46 or 4096 bp; a 4 bp recognition site will occur roughly every 256 bp.
Different restriction enzymes can have the same recognition site—such enzymes are called isoschizomers. Table IB shows that the recognition sites for SacI and SstI are identical. In some cases isoschizomers cut identically within their recognition site, but sometimes they do not. Isoschizomers often have different optimum reaction conditions, stabilities and costs, which may influence the decision of which to use. Table IB is provided only to show exemplary restriction enzymes, and does not limit the scope of the invention in any way.










TABLE IB








Recognition



Enzyme
Sequence









BamH I
GGATCC




CCTAGG



 



Not I
GCGGCCGC




CGCCGGCG



 



Sau3A I
GATC




CTAG



 



Sac I
GAGCTC




CTCGAG



 



Sst I
GAGCTC




CTCGAG



 



Hinf I
GANTC




CTNAG



 



Xho II
PuGATCPy




PyCTAGPu










Restriction recognitions sites can be unambiguous or ambiguous. The enzyme BamHI recognizes the sequence GGATCC and no others; therefore it is considered “unambiguous.” In contrast, HinfI recognizes a 5 bp sequence starting with GA, ending in TC, and having any base between (in Table IB, “N” stands for any nucleotide). HinfI has an ambiguous recognition site. XhoII also has an ambiguous recognition site: Py stands for pyrimidine (T or C) and Pu for purine (A or G), so XhoII will recognize and cut sequences of AGATCT, AGATCC, GGATCT and GGATCC.
The recognition site for one enzyme may contain the restriction site for another. For example, note that a BamHI recognition site contains the recognition site for Sau3AI. Consequently, all BamHI sites will cut with Sau3AI. Similarly, one of the four possible XhoII sites will also be a recognition site for BamHI and all four will cut with Sau3AI.
Also from Table IB, most recognition sequences are palindromes—they read the same forward (5′ to 3′ on the top strand) and backward (5′ to 3′ on the bottom strand). Most, but certainly not all recognition sites for commonly-used restriction enzymes are palindromes. Most restriction enzymes bind to their recognition site as dimers (pairs).
Nucleic Acid Detection
Whether detecting sequence differences, detecting amplification products or primer extension products, any detection or discrimination method known in the art may be utilized. These methods include, but are not limited to, primer extension reactions, mass spectrometry, hybridization using at least one probe, hybridization using at least one fluorescently labeled probe, direct sequencing, cloning and sequencing, and electrophoresis. Polymorphism detection methods known in the art may also include, for example, microsequencing methods, ligase sequence determination methods (e.g., U.S. Pat. Nos. 5,679,524 and 5,952,174, and WO 01/27326), digital PCR (U.S. Pat. No. 6,143,496), mismatch sequence determination methods (e.g., U.S. Pat. Nos. 5,851,770; 5,958,692; 6,110,684; and 6,183,958), microarray sequence determination methods, restriction fragment length polymorphism (RFLP) procedures, PCR-based assays (e.g., TAQMAN®PCR System (Applied Biosystems)), nucleotide sequencing methods, hybridization methods, conventional dot blot analyses, single strand conformational polymorphism analysis (SSCP, e.g., U.S. Pat. Nos. 5,891,625 and 6,013,499; Orita et al., Proc. Natl. Acad. Sci. U.S.A 86: 27776-2770 (1989)), denaturing gradient gel electrophoresis (DGGE), heteroduplex analysis, mismatch cleavage detection, and techniques described in Sheffield et al., Proc. Natl. Acad. Sci. USA 49: 699-706 (1991), White et al., Genomics 12: 301-306 (1992), Grompe et al., Proc. Natl. Acad. Sci. USA 86: 5855-5892 (1989), and Grompe, Nature Genetics 5: 111-117 (1993), detection by mass spectrometry (e.g., US 20050079521, which is hereby incorporated by reference), real time-PCR (e.g., U.S. Pat. No. 5,210,015, U.S. Pat. No. 5,487,972, both of which are hereby incorporated by reference), or hybridization with a suitable nucleic acid primer specific for the sequence to be detected. Suitable nucleic acid primers can be provided in a format such as a gene chip.
Primer extension polymorphism detection methods, also referred to herein as “microsequencing” methods, typically are carried out by hybridizing a complementary oligonucleotide to a nucleic acid carrying the polymorphic site. In these methods, the oligonucleotide typically hybridizes adjacent to the polymorphic site. As used herein, the term “adjacent” refers to the 3′ end of the extension oligonucleotide being sometimes 1 nucleotide from the 5′ end of the polymorphic site, often 2 or 3, and at times 4, 5, 6, 7, 8, 9, or 10 nucleotides from the 5′ end of the polymorphic site, in the nucleic acid when the extension oligonucleotide is hybridized to the nucleic acid. The extension oligonucleotide then is extended by one or more nucleotides, often 1, 2, or 3 nucleotides, and the number and/or type of nucleotides that are added to the extension oligonucleotide determine which polymorphic variant or variants are present. Oligonucleotide extension methods are disclosed, for example, in U.S. Pat. Nos. 4,656,127; 4,851,331; 5,679,524; 5,834,189; 5,876,934; 5,908,755; 5,912,118; 5,976,802; 5,981,186; 6,004,744; 6,013,431; 6,017,702; 6,046,005; 6,087,095; 6,210,891; and WO 01/20039. The extension products can be detected in any manner, such as by fluorescence methods (see, e.g., Chen & Kwok, Nucleic Acids Research 25: 347-353 (1997) and Chen et al., Proc. Natl. Acad. Sci. USA 94/20: 10756-10761 (1997)) and by mass spectrometric methods (e.g., MALDI-TOF mass spectrometry). Oligonucleotide extension methods using mass spectrometry are described, for example, in U.S. Pat. Nos. 5,547,835; 5,605,798; 5,691,141; 5,849,542; 5,869,242; 5,928,906; 6,043,031; 6,194,144; and 6,258,538.
Microsequencing detection methods often incorporate an amplification process that proceeds the extension step. The amplification process typically amplifies a region from a nucleic acid sample that comprises the polymorphic site. Amplification can be carried out by utilizing a pair of oligonucleotide primers in a polymerase chain reaction (PCR), in which one oligonucleotide primer typically is complementary to a region 3′ of the polymorphism and the other typically is complementary to a region 5′ of the polymorphism. A PCR primer pair may be used in methods disclosed in U.S. Pat. Nos. 4,683,195; 4,683,202, 4,965,188; 5,656,493; 5,998,143; 6,140,054; WO 01/27327; and WO 01/27329 for example. PCR primer pairs may also be used in any commercially available machines that perform PCR, such as any of the GENEAMP® Systems available from Applied Biosystems.
A microarray can be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample. A microarray may include any oligonucleotides described herein, and methods for making and using oligonucleotide microarrays suitable for prognostic use are disclosed in U.S. Pat. Nos. 5,492,806; 5,525,464; 5,589,330; 5,695,940; 5,849,483; 6,018,041; 6,045,996; 6,136,541; 6,142,681; 6,156,501; 6,197,506; 6,223,127; 6,225,625; 6,229,911; 6,239,273; WO 00/52625; WO 01/25485; and WO 01/29259. The microarray typically comprises a solid support and the oligonucleotides may be linked to this solid support by covalent bonds or by non-covalent interactions. The oligonucleotides may also be linked to the solid support directly or by a spacer molecule. A microarray may comprise one or more oligonucleotides complementary to a polymorphic site within a nucleotide sequence.
EXAMPLES
The following examples are provided to further describe embodiments of the invention and are not limiting.
Example 1
Restriction Endonuclease Enhanced Polymorphic Sequence Detection Using Hpych4v and NlaIII
The effectiveness of restriction endonuclease enhanced polymorphic sequence detection was demonstrated using several restriction endonucleases (REs), including HpyCH4V and NlaIII (purchased from New England BioLabs, Inc). Both of these enzymes were separately tested in multiplexed genotyping reactions for their ability to specifically cleave one allele of a given polymorphism while allowing PCR amplification of the remaining allele of the polymorphism. See Table 2 for the polymorphisms tested with each enzyme.
Two CEPH DNA samples were mixed in varying ratios to generate DNA samples composed of 0%, 2%, 5%, 20%, 50% and 100% DNA heterozygous for both alleles of the SNP, with the remaining DNA being homozygous for the allele recognized by the RE. Table 3 shows DNA samples used in these studies and corresponding genotype information. Mixtures composed of NA05995 and NA10849 were used for experiments with HpyCH4V enzyme, and mixtures composed of NA10862 and NA10846 were used for experiments with NlaIII enzyme.







TABLE 2







Restriction enzymes recognizing SNPs












SNP
Allele Digested


Restriction Enzyme
Polymorphism
Alleles
by RE






rs10430091
A/T



NlaIII
rs2050927
A/T
A


NlaIII, HpyCH4V
rs4329520
A/T
T, T*



rs4657868
A/T



HpyCH4V
rs4658481
A/T
A



rs6693568
A/T




rs860954
A/T




rs9431593
A/T





*Both enzymes, NlaIII and HpyCH4V, digest the T allele.













TABLE 3







DNA samples used and genotypes









SNP genotypes











Restriction Enzyme
DNA*
rs2050927
rs4329520
rs4658481





HpyCH4V
NA05995

TA
TA



NA10849

T
A


NlaIII
NA10862
AT
TA




NA10846
A
T





*DNA samples were obtained from Coriell CEPH DNA collection













TABLE 4







DNA mixtures (Listed as ng DNA per reaction)









Relative percentage unrecognized SNP allele














0%
2%
5%
20%
50%
100%


















HpyCH4V
NA05995
0
0.6
0.6
0.6
0.6
0.6



NA10849
0.6
29.4
11.4
2.4
0.6
0


NlaIII
NA10862
0
0.6
0.6
0.6
0.6
0.6



NA10846
0.6
29.4
11.4
2.4
0.6
0





NOTE:


Based on 3 pg DNA for haploid human genomic equivalent, 0.6 ng DNA is equal to 200 copies of genomic target DNA in the mixtures.






After preparation of the sample DNA mixtures, PCR cocktail was prepared according to Table 5 below (using multiplexed PCR primers as shown in Table 6) to either include no restriction endonuclease or 0.25 U of restriction endonuclease per each sample reaction. PCR cocktail was aliquoted to a 96-well plate to include 7 replicates of each DNA mixture for each enzyme condition. After addition of DNA to the PCR cocktail mixtures, samples were incubated at 37° C. for 1 hour to allow enzyme digestion of DNA samples and then immediately thermal cycled using standard conditions (Table 7).







TABLE 5







PCR cocktail preparation for each multiplex without DNA addition













No RE
HpyCH4V
NlaIII



Final
N =
N =
N =


Reagents
Conc
1 (uL)
1 (uL)
1 (uL)














Water
n/a
3
2.95
2.975


10xPCR Buffer
1.25x
3.125
3.125
3.125


(HotStar Taq Buffer)
















MgCl2 (25 mM)
1.625
mM
1.625
1.625
1.625


PCR Nucleotide Mix
0.2
mM
0.5
0.5
0.5


(for UNG use) (10 mM







dATP, dCTP, dGTP,







dUTP)







F/R Primer mix (0.5 uM)
0.1
μM
5
5
5


5 U/ul HpyCH4V or 10
0.25
U/rxn
—
0.05
0.025


U/ul NlaIII







1 U/μl Uracil-DNA-
1.25
U/rxn
1.25
1.25
1.25


Glycosylase (UDG)







HotStar Taq (5 U/uL)
2.5
U/rxn
0.5
0.5
0.5











DNA - added separately
varies
10
10
10


Total volume
n/a
25
25
25
















TABLE 6







PCR Primer sequences for SNPs













SEQ

SEQ


SNP
Forward PCR Primer
ID NO:
Reverse PCR Primer
ID NO:





rs10430091
ACGTTGGATGCACAAGA
4
ACGTTGGATGGCTGTTTA
12



TTCTGAAACTTAG

ACTCAGCATG



 


rs2050927
ACGTTGGATGTTGGGTG
5
ACGTTGGATGTTCTAGCT
13



CAGAGTAGTCATC

TGCTTCTCCTCC



 


rs4329520
ACGTTGGATGATGTCCA
6
ACGTTGGATGGAAAGTTG
14



CCTCCTGCTCCAC

TCGTGGTAGAGG



 


rs4657868
ACGTTGGATGCTAGCGT
7
ACGTTGGATGCTAACCAG
15



ACCCAATGGAATC

GAAAAGACACCC



 


rs4658481
ACGTTGGATGGTGGTAG
8
ACGTTGGATGCTGCTAAG
16



AAACAAATGTCAGC

CATGAGAGAAAG



 


rs6693568
ACGTTGGATGGGCCTGT
9
ACGTTGGATGTGACTAGG
17



TCATTCTCAGAAA

AAATCACACTGG



 


rs860954
ACGTTGGATGTAGCCTT
10
ACGTTGGATGCCATTCTT
18



TAGTCTTGATGCC

GTATGTTTTGTC



 


rs9431593
ACGTTGGATGGCCTCAG
11
ACGTTGGATGTTGAGATC
19



TAGTCACATAAGG

AGTGTCGGTTCC










Extend Primers









SNP
Extend Primer
SEQ ID NO:





rs10430091
gTGTTTAACTCAGCATGTGGGAA
20


 


rs2050927
CCTCCATCATCCTTAGC
21


 


rs4329520
GCGTGGTTCTAGACTTATGC
22


 


rs4657868
cAAGACACCCCCATACATTA
23


 


rs4658481
TAAGCATGAGAGAAAGGGAAAG
24


 


rs6693568
atGAAATCACACTGGACATTTT
25


 


rs860954
GTTTTGTCTTTTTCTGTATACTCATG
26


 


rs9431593
TGTTCCTGACTCTCAAAAT
27
















TABLE 7







Thermal cycling conditions












Temp.

Time
Cycles















37° C.
1
hour
1



94° C.
15
min
1



94° C.
20
sec
45 cycles



56° C.
30
sec




72° C.
1
min




72° C.
3
min
1












 4° C.

forever
1









Amplicon generated during PCR was genotyped with the extend primers in Table 5 using standard iPLEXTM assay and MassARRAY® technology (Jurinke, C., Oeth, P., van den Boom, D., MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis. Mol. Biotechnol. 26, 147-164 (2004); and Oeth, P. et al., iPLEXTM Assay: Increased Plexing Efficiency and Flexibility for MassARRAY® System through single base primer extension with mass-modified Terminators. SEQUENOM Application Note (2005), both of which are hereby incorporated by reference).
Results
Digestion of DNA with both restriction enzymes allowed detection of minor alleles when they were present at ratios as low as 2% heterozygous DNA. This is in contrast to undigested DNA samples where minor alleles were only reliably detected when present at ratios of 20% heterozygous DNA and higher. When allele peak area ratios are considered, the effect of restriction endonuclease digest is even more apparent. HpyCH4V digested samples showed minor allele peak area ratios of 0.35-0.45 in 2% heterozygous DNA mixtures, while minor allele peak area ratios of 2% heterozygous DNA mixtures were at background levels without enzyme digestion (FIG. 1). While the increases in allele peak area ratio were not as high when using the NlaIII restriction endonuclease, the results were similar (FIG. 2). Example screen shots of the mass spectrum in 2% heterozygous DNA mixtures with and without HpyCH4V (FIG. 3) or NlaIII (FIG. 4) are shown below.
Optimization Studies
Initial optimization studies for enzyme concentration and pre-PCR incubation time of HpyCH4V digestion were performed using 5% heterozygous DNA mixtures (0.6 ng heterozygous DNA, 11.4 ng homozygous DNA). Based on these experiments, maximal peak area ratios were obtained with incubation times as short as 5 minutes and 0.25 U HpyCH4V enzyme.
Example 2
Restriction Endonuclease Enhanced Polymorphic Sequence Detection Using Tfii
A similar experiment was performed as described in Example 1 using a different restriction endonuclease, TfiI. In this experiment, the TfiI restriction endonuclease selectively recognized and cleaved the ‘C’ allele of the ‘C/T’ SNP, rs4487973. The SNP rs4487973 occurs in the following genomic sequence on chromosome 1: CACACAGTTAGGATT[C/T]ACCTGAGCTTGTCCC (SEQ ID NO: 28). For these studies, two CEPH DNA samples, one homozygous ‘C’ and the other heterozygous ‘C/T’ for the rs4487973 SNP, were mixed in varying ratios to generate DNA mixtures containing 0%, 1%, 2.5%, 10%, 50% of the rs4487973 ‘T’ allele. The TfiI restriction endonuclease was either added or not added to each mixture to determine the endonucleases' effect on detecting the polymorphic sequence. Of the mixtures not digested with TfiI enzyme, the rs4487973 ‘T’ allele was detected in the 10%, and 50% ‘T’ allele mixtures, but not the 0%, 1%, and 5% ‘T’ allele DNA mixtures. However, of samples digested with TfiI enzyme, the rs4487973 ‘T’ allele was detectable in 1%, 5%, 10% and 50% ‘T’ allele mixtures. These results indicate the utility of this method to improve detection of polymorphic alleles present at low relative concentrations in a sample.
Example 3
Fetal Identifiers, Sex Test And Copy Number Determination
Selection of SNPs
Analysis of paternally-inherited alleles in clinical samples and correlation with Y-chromosome frequency in male fetuses was performed with a total of 16 SNPs. SNP assays for analysis of clinical samples were multiplexed as 8-plexes. All SNPs had a minor allele frequency (maf) of  ̃0.4 in all ethnic groups and were unlinked.
For performance evaluation of a universal Fetal Identifier panel that can be multiplexed with disease-specific markers, a new panel of 87 NT SNPs with a pan-ethnic maf >0.4 was selected and multiplexed into 16-plexes.
Method of SNP Analysis
Analysis of SNPs in maternal buffy coat and maternal plasma was performed using the iPLEXTM assay and MassARRAY® technology. In brief, the target region surrounding the SNP is first amplified by PCR. Subsequently an oligonucleotide primer is annealed to the PCR product and is extended allele-specifically by a single nucleotide using a mixture of 4 terminator nucleotides and a DNA polymerase. The extension products are transferred to a miniaturized chip array and are analyzed by MALDI-TOF Mass Spectrometry. Determination of the molecular mass of extension products allows unambiguous identification of the SNP allele present in the sample. The peak area ratio of mass signals allows the estimation of the relative abundance of the alleles in a given sample. FIG. 5A provides an overview of the assay used for SNP analysis.
Clinical Samples
The total sample set consisted of 35 paired blood/plasma samples from pregnant Caucasian woman (nine 1st trimester; twelve 2nd trimester; fourteen 3rd trimester).
The subset of samples used for correlation of Y-chromosome frequency and paternally-inherited alleles in maternal plasma consisted of 19 samples of pregnant Caucasian woman carrying a male fetus.
DNA Extraction
DNA extraction was performed from 1 ml of maternal plasma using the Qiagen® MinElute kit for fetal genotyping.
DNA extraction from frozen blood (minus plasma) was performed from 4 ml using Qiagen's PureGene kit for maternal genotyping.
Results
An assay targeting sequence differences in the Amelogenin region on the X and Y chromosome was used to assess the relative amount of fetal DNA extracted from plasma of pregnant woman carrying a male fetus. Details of the AMG assay are depicted in FIGS. 8A-8C. X and Y-specific sequences can be discriminated by sequence specific iPLEX extension products and their respective mass signals. The peak area ratio of the extension products allows estimation of the relative amount of fetal DNA, because the Y-specific sequences represent 50% of the total fetal DNA contribution.
Sixteen of nineteen (84%) plasma samples with a male fetus showed a Y-chromosome frequency of higher than 5%, indicating presence of at least 10% fetal DNA in the extracted DNA.
FIG. 6 depicts typical performance results for a qualified fetal identifier. Here the ability of the SNP assay to estimate the quantity of fetal DNA in the background of maternal DNA was verified for a total of 1700 copies and a total of 170 copies using genomic DNA mixtures. Note that the standard deviation of the estimate of fetal DNA increases due to the significant influence of the sampling error at low copy numbers
Table 8 provides a list of SNPs that were multiplexed at 10+ plexing level and passed all phases of the validation. The following shows the validation scheme, performance criteria and model system used to qualify multiplex SNP assays for their utility in identifying the presence for fetal DNA:
Phase I


    
    
        Step 1: Initial Fetal Identifier (FI) screening parameters
        
            F1's are multiplexed from pool of 87 A/T SNPs (mass difference 56 Da)
            Genotyping of control DNAs (CEPH populations)
        
        
        Step 2: Advance screening criteria
        
            Reproducibility of genotyping calls in 4 replicates
            Unambiguous genotype data (assay shows no interfering or unpredicted mass signals)
            Allelic skew in heterozygous DNAs
            Variance of allelic ratio in heterozygous DNAs
        
        
        Step 3: Replex successful SNPs and repeat Phase 1 screening to generate multiplexes of 10+ SNPs

Multiplexed SNPs passing Phase I test criteria are tested in Phase II

Phase II

        Step 1: Mixtures of Genomic DNA are used for assessing FI reliability
        
            Mix Mother: 2000 copies of DNA1
            Mix 10%: 3600 copies DNA 1/400 copies of DNA 2
            Mix 20%: 1600 copies DNA 1/400 copies of DNA 2
        
        
        Analysis of allele frequency variation in 4 mixture series and 8 replicate measurements. Sensitivity and specificity are calculated for the detection of low copy number allele in background of high copy number allele

Multiplexed SNPs passing Phase II test criteria are tested in Phase III

Phase III

        Step 1: Various DNAs are mixed to emulate different maternal-fetal combinations
        
            Plate 1: 3600 copies DNA maternal/400 copies DNA fetal
            Plate 2: 1600 copies DNA maternal/400 copies DNA fetal
            Each plate contains 88 sample mixtures, 4 positive and 4 negative controls. Analysis of allele frequency variation in 4 mixture series, where sensitivity and specificity are calculated for the detection of low copy number allele in background of high copy number allele
        
        
    
    


Application of this assay panel to a model system for the detection of fetal DNA in maternal background showed that paternally-inherited fetal alleles can be detected with a sensitivity of 95% at 100% specificity if the sample preparation method can enrich the relative amount of fetal DNA to 20%. In Table 8, the minor allele frequency (MAF) for each SNP from different ethnic populations is provided. The ethnic populations are defined by the HapMap Project, where CEU represents individuals of Northern and Western Europe descent, HCB represents Han Chinese in Beijing, JAP represents Japanese in Tokyo, and YRI represents the Yoruba in Ibadan, Nigeria.












TABLE 8







MAF
MAF
MAF
MAF



SNP
CEU
HCB
JAP
YRI





















rs11166512
0.43
0.41
0.50
0.49



rs11184494
0.50
0.40
0.48
0.50



rs11247894
0.43
0.39
0.32
0.44



rs12089156
0.46
0.49
0.44
0.43



rs12125888
0.40
0.43
0.48
0.43



rs12136370
0.42
0.48
0.42
0.48



rs12143315
0.40
0.42
0.42
0.42



rs12759642
0.39
0.48
0.48
0.42



rs156988
0.46
0.40
0.45
0.41



rs2050927
0.44
0.50
0.41
0.49



rs213624
0.48
0.44
0.40
0.34



rs2454175
0.46
0.48
0.43
0.40



rs4329520
0.45
0.43
0.40
0.44



rs4487973
0.47
0.43
0.44
0.40



rs454782
0.48
0.40
0.41
0.46



rs4648888
0.33
0.30
0.33
0.46



rs635364
0.49
0.40
0.46
0.43



rs660279
0.41
0.49
0.50
0.39



rs6687785
0.48
0.46
0.48
0.44



rs7551188
0.46
0.49
0.45
0.46



rs9431593
0.41
0.43
0.49
0.40









A multiplexed panel of 16 SNPs was analyzed with maf>0.3 in the same maternal plasma DNA extraction and established a baseline of maternal genotypes by analyzing DNA from PBMCs. Using the maternal genotype information, paternally-inherited alleles were identified in plasma samples and estimated the amount of fetal DNA from the peak area ratio of extension products representing paternally-inherited fetal alleles and maternal alleles.
The AMG XY frequency was then compared with the allele-frequency of paternally-inherited fetal alleles in informative SNPs. This comparison revealed that samples with a positive Y-frequency of 10% (used as a Limit-of-quantitation threshold) or more have significantly higher differences between maternally and paternally-inherited fetal allele-frequencies (p-value <0.001; Fishers' exact test). This data suggests that Fetal Identifiers can be used as a non-gender specific approach for identification of the presence of fetal DNA. FIG. 7 exemplifies those results.














TABLE 9








SEQ

SEQ

SEQ


Multi-
Primer

ID

ID
Extend
ID


plex
Name
Amplification primer
NO:
Amplification primer
NO:
Primer sequence
NO:







MP1
rs7551188
ACGTTGGATGATCCCTGGTTCCTT
29
ACGTTGGATGGAGCCTCTCAGTG
51
GGACAGATTCTGGGAC
73




CCTTAG

TCTATAC





 


MP1
rs11247894
ACGTTGGATGATCCTAGATAGCC
30
ACGTTGGATGGGAGGAAAGAGAA
52
CCAAAGCCAAGAATTCA
74




CAAAGCC

GATTGTG





 


MP1
rs6687785
ACGTTGGATGATGCTGTAAAGAG
31
ACGTTGGATGTTCTCCTCTGACC
53
CCTCAACAGTACACTTAATC
75




CCTCAAC

TGCTTTC





 


MP1
rs4487973
ACGTTGGATGTCAGAGAGTGACA
32
ACGTTGGATGGAATGCATGCCAA
54
cAGGTCACACAGTTAGGATT
76




AGACCTG

CTTAGGG





 


MP1
rs4648888
ACGTTGGATGCAGAGAGTCCCCT
33
ACGTTGGATGTGCCCAGACCAGA
55
aTGGACCTTCGGAAAGGATA
77




GTTATTG

GAGGTCA





 


MP1
rs12089156
ACGTTGGATGGCTACATACTATGT
34
ACGTTGGATGCCTGCTGGCAACA
56
TACTATGTGGTCTCAACTATA
78




GGTCTC

AATCTTC

T



 


MP1
rs2050927
ACGTTGGATGTTCTAGCTTGCTTC
35
ACGTTGGATGTTGGGTGCAGAGT
57
TGCTTCTCCTCCATCATCCTT
79




TCCTCC

AGTCATC

AGC



 


MP1
rs12125888
ACGTTGGATGCAACATCCTGTAC
36
ACGTTGGATGAGACAATTTCTGT
58
TACATGACTATCTCCTCCCTT
80




ATCACTC

CCTCTGG

AGGT



 


MP1
rs12143315
ACGTTGGATGACAGGCATGAGCC
37
ACGTTGGATGTGCCATTGGTACA
59
CCATCTTACCCAGCCTCTTTC
81




ATCTTAC

GTCACTC

TTCAA



 


MP1
rs213624
ACGTTGGATGTAGGTCAAGCCAA
38
ACGTTGGATGTGTCCACCCAGGA
60
gGCCAAGGCCTCGGAGTCTG
82




GGCCTC

GCAGCCA

AACAGTT



 


MP1
SRY_5-ib
ACGTTGGATGAGCATCTAGGTAG
39
ACGTTGGATGAGCAACGGGACC
61
cGTTACCCGATTGTCCTAC
83




GTCTTTG

GCTACAG





 


MP2
rs660279
ACGTTGGATGTTTCAGCAACCACT
40
ACGTTGGATGTGCCCGTAAGTAG
62
CTTGATGTGCTTCCCTG
84




CTGAGC

GAGAGTG





 


MP2
rs635364
ACGTTGGATGGAAATTTCTGGATT
41
ACGTTGGATGAGAGACTCCATTT
63
TGGATTACTGGCAAAGAC
85




ACTGGC

GTTTGGG





 


MP2
rs9431593
ACGTTGGATGTTGAGATCAGTGT
42
ACGTTGGATGGCCTCAGTAGTCA
64
TGTTCCTGACTCTCAAAAT
86




CGGTTCC

CATAAGG





 


MP2
rs11166512
ACGTTGGATGCTTCATCCACTATA
43
ACGTTGGATGTGACCAGATGTTG
65
CCACTATATCCACCTTTTCT
87




TCCACC

GATTAG





 


MP2
rs4329520
ACGTTGGATGGAAAGTTGTCGTG
44
ACGTTGGATGATGTCCACCTCCT
66
GCGTGGTTCTAGACTTATGC
88




GTAGAGG

GCTCCAC





 


MP2
rs454782
ACGTTGGATGCTGTTAAGATGCC
45
ACGTTGGATGCTGTCTTCCTCATT
67
AACTCCCATATTAGTCCACAG
89




AACTCCC

GCTCTG





 


MP2
rs12136370
ACGTTGGATGGAGTAGTTCTTTG
46
ACGTTGGATGCTCCTGGAAAACA
68
gGCAGTAAGCTATTCTTGGGG
90




CAGTAAGC

GCAAAAG





 


MP2
rs12759642
ACGTTGGATGATTCTTCCTGGGA
47
ACGTTGGATGGGAAATACCAGCA
69
caTCGGGATTCCCTGAACAAA
91




CTCAGAC

ACCACAG

A



 


MP2
rs11184494
ACGTTGGATGAGCTGGCCATGTT
48
ACGTTGGATGGCCAATCTATGAA
70
ATTTGACTTTCCTACTCCTTA
92




TATTTGAC

GAATTAC

AC



 


MP2
rs2454175
ACGTTGGATGGGAATCAGACCTG
49
ACGTTGGATGGCCCAGCAGGAC
71
cCTTCAAGGATTGGAATTAGA
93




TAAACAC

ACTTTTAT

GT



 


MP2
rs156988
ACGTTGGATGAAAGCTCTGTGAT
50
ACGTTGGATGGAAAGGGCTATGT
72
tCGTCTCGGTCCTTCCTTTTC
94




GCGTCTC

AAGGAGG

ACTT























TABLE 10








SEQ

SEQ

SEQ


Multi-


ID

ID
Extend
ID


plex
SNP_ID
Amplification primer
NO:
Amplification primer
NO:
Primer sequence
NO:






















W1
rs10793675
ACGTTGGATGAAGAGATGAGACA
 95
ACGTTGGATGCTCTGTATTTATAG
134
AACGGCTCAACAGTT
173




GACTGGG

CTTTC





 


W1
rs1829309
ACGTTGGATGATCTCTGAGTTGA
 96
ACGTTGGATGTTCCTAATCAGGAG
135
TTGCTTTGGGGAGCAG
174




CACCACC

AGACCG





 


W1
rs660279
ACGTTGGATGTTTCAGCAACCACT
 97
ACGTTGGATGTGCCCGTAAGTAG
136
CTTGATGTGCTTCCCTG
175




CTGAGC

GAGAGTG





 


W1
rs635364
ACGTTGGATGGAAATTTCTGGATT
 98
ACGTTGGATGAGAGACTCCATTTG
137
TGGATTACTGGCAAAGAC
176




ACTGGC

TTTGGG





 


W1
rs9431593
ACGTTGGATGTTGAGATCAGTGT
 99
ACGTTGGATGGCCTCAGTAGTCA
138
TGTTCCTGACTCTCAAAAT
177




CGGTTCC

CATAAGG





 


W1
rs11166512
ACGTTGGATGCTTCATCCACTATA
100
ACGTTGGATGTGACCAGATGTTG
139
CCACTATATCCACCTTTTCT
178




TCCACC

GATTAG





 


W1
rs4329520
ACGTTGGATGGAAAGTTGTCGTG
101
ACGTTGGATGATGTCCACCTCCTG
140
GCGTGGTTCTAGACTTATGC
179




GTAGAGG

CTCCAC





 


W1
rs454782
ACGTTGGATGCTGTTAAGATGCC
102
ACGTTGGATGCTGTCTTCCTCATT
141
AACTCCCATATTAGTCCACA
180




AACTCCC

GCTCTG

G



 


W1
rs12136370
ACGTTGGATGGAGTAGTTCTTTG
103
ACGTTGGATGCTCCTGGAAAACA
142
gGCAGTAAGCTATTCTTGGG
181




CAGTAAGC

GCAAAAG

G



 


W1
rs12759642
ACGTTGGATGATTCTTCCTGGGA
104
ACGTTGGATGGGAAATACCAGCA
143
caTCGGGATTCCCTGAACAA
182




CTCAGAC

ACCACAG

AA



 


W1
rs11184494
ACGTTGGATGAGCTGGCCATGTT
105
ACGTTGGATGGCCAATCTATGAAG
144
ATTTGACTTTCCTACTCCTTA
183




TATTTGAC

AATTAC

AC



 


W1
rs2454175
ACGTTGGATGGGAATCAGACCTG
106
ACGTTGGATGGCCCAGCAGGACA
145
cCTTCAAGGATTGGAATTAGA
184




TAAACAC

CTTTTAT

GT



 


W1
rs1452628
ACGTTGGATGGCTTGTGCTTTGTT
107
ACGTTGGATGGGTCAAGCAAAGG
146
acatAGTTATTCCTAGGGCTT
185




GTGTGG

CTTCAAG

CTC



 


W1
rs156988
ACGTTGGATGAAAGCTCTGTGAT
108
ACGTTGGATGGAAAGGGCTATGT
147
tCGTCTCGGTCCTTCCTTTTC
186




GCGTCTC

AAGGAGG

ACTT



 


W1
rs4570430
ACGTTGGATGACCCGAGCCAATC
109
ACGTTGGATGGCACATGGAGATG
148
GGTATCATAAGATACCTATG
187




AGGTATC

AATGGTC

ATGTC



 


W1
rs12062414
ACGTTGGATGTGCGTCAACCTTT
110
ACGTTGGATGGGAAAGTCCTCGA
149
ggaaTTTCCAGTTCTATTCCA
188




CCAGTTC

CTGTTTG

GCCTC



 


W1
rs7545381
ACGTTGGATGCCAGTCAAGCTAA
111
ACGTTGGATGGTGAGCACAACTG
150
tccCTGAATGACAAAAGGGGA
189




GGACAAA

TGTTCTA

AGATA



 


W1
rs6427673
ACGTTGGATGGGACTAAAACAGG
112
ACGTTGGATGGTCTCTCTAGTACT
151
ccctcGCCAAACTTAGACCAA
190




GCCAAAC

AGTAAC

GGACAAC



 


W1
rs10802761
ACGTTGGATGTCTTCTAAAATGTA
113
ACGTTGGATGGGATGAGGTTTTG
152
AGTTATGAAATAAGTTTTATT
191




GTTATG

ACTAAGC

CATTTAC



 


W2
rs642449
ACGTTGGATGCCAAAAAACCATG
114
ACGTTGGATGAGATTGCCTCTCCA
153
CCTCTGCCTCCCCTA
192




CCCTCTG

TGTGAC





 


W2
rs4839419
ACGTTGGATGCTGCCGCATCCCT
115
ACGTTGGATGATGTGTTTGTGGCC
154
CCTTCACAAAGCCGA
193




TCACAA

ACTTCC





 


W2
rs9324198
ACGTTGGATGAAAGGCCTACTGT
116
ACGTTGGATGCAAAATATGTGTGA
155
cGTTTGCTGGAAGCCT
194




TTGCTGG

ATCAGC





 


W2
rs1192619
ACGTTGGATGGCTCAACTCTGAA
117
ACGTTGGATGCCAGGAATGGGCA
156
TGGCCAGAAGAAGGAG
195




CCAATCG

TGTGTTC





 


W2
rs4657868
ACGTTGGATGCTAACCAGGAAAA
118
ACGTTGGATGCTAGCGTACCCAAT
157
AGACACCCCCATACATTA
196




GACACCC

GGAATC





 


W2
rs6426873
ACGTTGGATGTAAATCAGGGCTG
119
ACGTTGGATGAAGTGCTAGGGTT
158
cccCTGCCTTCTCTTCCAA
197




CCTTCTC

ACAGGTG





 


W2
rs438981
ACGTTGGATGTGTGCAAATTGGC
120
ACGTTGGATGGAACATTGGTATTT
159
ATGGACCACAAAAAACTTA
198




TAACAT

AAACTC





 


W2
rs12125888
ACGTTGGATGAGACAATTTCTGTC
121
ACGTTGGATGCAACATCCTGTACA
160
TCTGTCCTCTGGTATCCTCT
199




CTCTGG

TCACTC





 


W2
rs3128688
ACGTTGGATGATCAAGAGGAAAA
122
ACGTTGGATGGATTTACTCAACTC
161
cAAAATGGACAGAAGTTGAA
200




TGGACAG

TCTGGG





 


W2
rs4987351
ACGTTGGATGGTGCATGGGCTCA
123
ACGTTGGATGCCAAACAGGGCCA
162
gCATCTAGACACATTTTGTGC
201




TCTAGAC

ATGGTAG





 


W2
rs6692911
ACGTTGGATGCTATTCCCTCCTCA
124
ACGTTGGATGATTAAGATGGGTAG
163
tccAAGAGCATTTTTCCTCTT
202




AAGAGC

TTAAG

C



 


W2
rs6684679
ACGTTGGATGTATGTTACTTGCCT
125
ACGTTGGATGTCTTAAGGTGTCTC
164
ggaCCACTGAGGAGATACAC
203




TGGCCC

CCTCTG

TA



 


W2
rs4320829
ACGTTGGATGGGTTCTATGGCTTT
126
ACGTTGGATGTGCTAGACACTTTA
165
ggtcACCTCTTTTCATAACAG
204




GGTGAG

ACTGCC

GA



 


W2
rs4658481
ACGTTGGATGCTGCTAAGCATGA
127
ACGTTGGATGGTGGTAGAAACAA
166
atacGCATGAGAGAAAGGGAA
205




GAGAAAG

ATGTCAGC

AG



 


W2
rs3768458
ACGTTGGATGCCAAATGTCTTAGT
128
ACGTTGGATGGAGTTTATGTAATG
167
CTTAGTTACAAAGAAAATTGT
206




TACAAAG

TCAAC

GAG



 


W2
rs860954
ACGTTGGATGTAGCCTTTAGTCTT
129
ACGTTGGATGCCATTCTTGTATGT
168
TCTTGATGCCTTACAAAATAA
207




GATGCC

TTTGTC

ATAT



 


W2
rs10453878
ACGTTGGATGGAGGAGCTAACAA
130
ACGTTGGATGGGGATATGAATTAC
169
AAACAAATCCTCCTTTCTTTT
208




GTAGGAC

AACAGAG

AATTC



 


W2
rs10753912
ACGTTGGATGGAGATTATATGTCT
131
ACGTTGGATGATTCTTCTAACTTTT
170
GAGATTATATGTCTCTTTAAT
209




CTTTAA

AGGC

ATTGTC



 


W2
rs1637944
ACGTTGGATGCTAATGCCTCCTTT
132
ACGTTGGATGAATAGCAAACAACA
171
cccccATATCATTTGCAATTG
210




TCTTCC

GGTGGG

CATGGTT



 


W2
rs4839282
ACGTTGGATGGAATCCTGGCAGC
133
ACGTTGGATGTGGGTTCACATGA
172
gatgTCTCTTAAAGAGCAAAA
211




TCATTAG

GTCTTGC

AGCTAAG






















TABLE 11







SEQ

SEQ

SEQ




ID

ID
Extend
ID


SNP ID
Amplification primer
NO:
Amplification primer
NO:
Primer sequence
NO:







rs4292357
ACGTTGGATGCTTACCCATGCTAAGT
212
ACGTTGGATGAAGATTATGGAGGCCA
233
TCCCACCTCCTCCGTGC
254



CCTG

GGAG





 


rs9940995
ACGTTGGATGTCTGGAATGCCCCTTC
213
ACGTTGGATGGAGCATATTCTGAGGA
234
GAACGTACCTCCCATGC
255



TTAG

TGGG





 


rs1122443
ACGTTGGATGTTCCTTTTCTTCCAGAT
214
ACGTTGGATGGTATATAGCTGGGCAT
235
GCCCAGGGTGAATTAAA
256



GGG

GGTG





 


rs10761670
ACGTTGGATGCTCTGTCACTGACTTC
215
ACGTTGGATGTACTCAGAGGAACCTG
236
TGACTTCATGGGACCTCA
257



ATGG

AGTC





 


rs2560643
ACGTTGGATGGGCTCTAAGCCATTCT
216
ACGTTGGATGGCCTTTCAAAGCCACA
237
tTCCCCCTGTCCCTCTGCC
258



TCTG

TCTC





 


rs13134004
ACGTTGGATGGGAGTTCTTATCCATA
217
ACGTTGGATGGGAAGCTTCTGGGAC
238
TTTTCTGTGCTTTTTCCTC
259



CTATG

TTAAT





 


rs11077442
ACGTTGGATGAACTCCCATTTCTCCC
218
ACGTTGGATGTGGACCCAGTCAAGA
239
gaTCCCTGTGTGGTAATGC
260



TGTG

AAGTC





 


rs4924176
ACGTTGGATGGGTTTCATTCCCAACA
219
ACGTTGGATGCAGCCTATGTATGGAA
240
AATGCCACATTCTTGATTGC
261



AGTC

ACAG





 


rs6005955
ACGTTGGATGTTAAGCACTGCCTGTA
220
ACGTTGGATGGGGCAGTGAACTTGT
241
ggaAGTGTGCTAGACGCTGC
262



TCCC

CTAC





 


rs9893096
ACGTTGGATGAACCCACTATACCCCA
221
ACGTTGGATGACACACACATTCTGCT
242
ccccAGGCCCAAGTCTTCTGC
263



ACTC

GAGG





 


rs7241823
ACGTTGGATGTAACTTGATTACTGGC
222
ACGTTGGATGCCAGGTGTGTCTCAAA
243
ggggACTGGCACTGCCCCATA
264



ACTG

ATTC





 


rs6026436
ACGTTGGATGCTCAAAGGTGCCAATC
223
ACGTTGGATGCTGATGGCTTGTCTTG
244
aCACAGGCCCATAAACTATAA
265



ACTC

ATTC

C



 


rs7926887
ACGTTGGATGCTGAACAAGACCTTGA
224
ACGTTGGATGGTTGAGACTGCCAGC
245
ggACAAGACCTTGAAGTTGA
266



AG

TATTC

TA



 


rs17811318
ACGTTGGATGTACAACTGGATGCTGA
225
ACGTTGGATGTTAGACATGAGGCCC
246
aAAAAGAGAGTTGAAGTTTA
267



GTAG

CAAAG

GG



 


rs578381
ACGTTGGATGTGTTTCCTTGAGGCTC
226
ACGTTGGATGAACAGGTCTGACATCT
247
gttgcGGCTCTCTGTTGTCT
268



TCTG

GTGG

GTT



 


rs978055
ACGTTGGATGTTCCAAGATCCCCCTT
227
ACGTTGGATGGGCATAAAGCCTTACA
248
gaaccTTTACTTGCTTCCTG
269



TTCC

CCTC

TTGC



 


rs17627583
ACGTTGGATGTGGATCTTACCCCTAT
228
ACGTTGGATGTTGGTGTGACCAAGAC
249
gTCAGATAAAGTCTGAGTTCA
270



TCAG

CTAC

TTG



 


rs2887761
ACGTTGGATGTCTGATGGTATGTAGA
229
ACGTTGGATGGGGCAAATTTCCATTT
250
ATGGTATGTAGAGAGGTAAAT
271



GAGG

GTG

TGC



 


rs7702915
ACGTTGGATGTCCCATAGTCTGATCC
230
ACGTTGGATGGATGTTCTCCAAAGTA
251
ggagaATCCCTACCTGATGTT
272



CTAC

GAAG

TTGC



 


rs4998490
ACGTTGGATGCCAGGAATTGGGATAT
231
ACGTTGGATGCCAAGCTTCAAATCAA
252
tCTTACCACTAAAAAACTATC
273



GGAC

TGTG

AGTAT



 


rs9306015
ACGTTGGATGGCCTGGGAAAATGATT
232
ACGTTGGATGGTGGGAGATATTGTTA
253
AATATTTTGAGATAAATTGTA
274



GGAC

AGGC

GATGC









The entirety of each patent, patent application, publication and document referenced herein hereby is incorporated by reference. Citation of the above patents, patent applications, publications and documents is not an admission that any of the foregoing is pertinent prior art, nor does it constitute any admission as to the contents or date of these publications or documents.
Modifications may be made to the foregoing without departing from the basic aspects of the invention. Although the invention has been described in substantial detail with reference to one or more specific embodiments, those of ordinary skill in the art will recognize that changes may be made to the embodiments specifically disclosed in this application, yet these modifications and improvements are within the scope and spirit of the invention.
The invention illustratively described herein suitably may be practiced in the absence of any element(s) not specifically disclosed herein. Thus, for example, in each instance herein any of the terms “comprising,” “consisting essentially of,” and “consisting of” may be replaced with either of the other two terms. The terms and expressions which have been employed are used as terms of description and not of limitation, and use of such terms and expressions do not exclude any equivalents of the features shown and described or portions thereof, and various modifications are possible within the scope of the invention claimed. The term “a” or “an” can refer to one of or a plurality of the elements it modifies (e.g., “a reagent” can mean one or more reagents) unless it is contextually clear either one of the elements or more than one of the elements is described. The term “about” as used herein refers to a value within 10% of the underlying parameter (i.e., plus or minus 10%), and use of the term “about” at the beginning of a string of values modifies each of the values (i.e., “about 1, 2 and 3” is about 1, about 2 and about 3). For example, a weight of “about 100 grams” can include weights between 90 grams and 110 grams. Thus, it should be understood that although the present invention has been specifically disclosed by representative embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and such modifications and variations are considered within the scope of this invention.
Embodiments of the invention are set forth in the claims that follow.

## Claims

1. A method for detecting the presence or absence of a plurality of target alleles at a plurality of polymorphic loci in a sample, wherein the sample contains maternal nucleic acid and fetal nucleic acid, which comprises:
a) cleaving nucleic acid comprising a plurality of non-target alleles at the plurality of polymorphic loci with a single cleavage agent that recognizes the non-target alleles at single-nucleotide polymorphic loci, wherein the cleavage agent is a non-thermostable restriction endonuclease;
b) elevating the temperature to a temperature that inactivates the endonuclease;
c) after (b), amplifying uncleaved nucleic acid but not cleaved nucleic acid; and
d) analyzing the amplification products of step (c) to determine the presence or absence of a plurality of target alleles, wherein
the target alleles are of paternal origin and the non-target alleles are of maternal origin.

2. The method of claim 1, wherein the sample is from a pregnant female or a female suspected of being pregnant.

3. The method of claim 1, wherein 60 or more polymorphic loci are assayed.

4. The method of claim 1, wherein 10 or more target alleles are detected.

5. The method of claim 1, wherein the non-thermostable restriction endonuclease is HpyCH4V.

6. The method of claim 1, wherein the target allele concentration is less than 4% of the non-target allele concentration prior to cleaving and amplifying the nucleic acid.

7. The method of claim 1, wherein the single nucleotide polymorphic loci are selected from the group consisting of the polymorphisms rs10430091, rs2050927, rs4329520, rs4657868, rs4658481, rs6693568, rs860954 and rs9431593.

8. The method of claim 1, wherein the relative amount of fetal nucleic acid is enriched to at least 20% in part (c).

9. The method of claim 8, wherein in part (d) the presence or absence of a plurality of target alleles is determined with a sensitivity of at least 95% and a specificity of about 100%.