# Patent

## Title
Branched copolymer/anthranilic diamide compositions for propagule coating

## Abstract

Disclosed is an insecticidal composition comprising by weight based on total weight of composition

    
    
        (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and
        (b) from about 9 to about 91% of a branched copolymer component having water solubility or dispersibility at least about 5% by weight at 20° C.,
        wherein:
        the branched copolymer component comprises one or more branch segments attached to linear backbone segment;
        the branch segments comprise at least two repeating monomer units and have number average molecular weight (Mn) at least about 1,000;
        the branched copolymer component comprises hydrophilic groups; and
        ratio of component (b) to component (a) is about 1:10 to about 10:1 by weight.
    
    


Also disclosed is a geotropic propagule coated with insecticidal composition. Further disclosed is a liquid composition comprising the insecticidal composition, and method for protecting a geotropic propagule and plant derived therefrom a phytophagous insect pest.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of the U.S. Provisional Application No. 61/422,261, filed on Dec. 13, 2010.


FIELD OF THE INVENTION
This invention relates to compositions comprising anthranilic diamide insecticides and branched copolymers. This invention also relates to geotropic propagules coated with these compositions and to protecting propagules and derived plants from phytophagous insect pests by contacting the propagules with these compositions.
BACKGROUND
Damage by phytophagous insect pests to geotropic propagules such as seeds, rhizomes, tubers, bulbs or corms, and plants derived therefrom causes significant economic losses.
Anthranilic diamides, alternatively called anthranilamides, are a recently discovered class of insecticides having activity against numerous insect pests of economic importance. PCT Publication WO 03/024222 discloses treatment with anthranilic diamides being useful for protecting propagules from phytophagous invertebrate pests. Furthermore, because of the ability of anthranilic diamides to translocate within plants, not only the propagules, but also new growth developing from the propagules can be protected.
Although anthranilic diamides have properties making them suitable for protecting propagules and developing growth, achieving sufficient absorption of anthranilic diamides into the propagule and developing roots to cause insecticidally effective concentrations in parts of the developing plant for which protection is desired can be problematical. Although anthranilic diamide coatings on propagules are exposed to moisture from the propagules and surrounding plant growing medium (e.g., soil), the low water solubility of anthranilic diamide insecticides impedes their mobilization through moisture. Also, until the anthranilic diamides are absorbed into the propagules and developing roots, they are vulnerable to absorption and dissipation through the growing medium.
Achieving insecticidally effective concentrations of anthranilic diamides in foliage by treating propagules requires greater amounts of anthranilic diamides to be available for transport greater distances within the plant. Because the rapidly expanding volume of plant tissue in growing foliage inherently dilutes anthranilic diamide concentrations, absorption of increased amounts of anthranilic diamides is required for protection of foliage, particularly if protection of foliage beyond the first couple leaves and during a substantial part of the growing season is desired.
Accordingly, need exists for new compositions promoting the absorption of anthranilic diamide insecticides into propagules and developing roots. Such compositions have now been discovered.
SUMMARY
One aspect of the present invention is an insecticidal composition comprising by weight based on the total weight of the composition:

    
    
        (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and
        (b) from about 9 to about 91% of a branched copolymer component having a water solubility or dispersibility of at least about 5% by weight at 20° C.,
        wherein:
        the branched copolymer component comprises one or more branch segments attached to a linear backbone segment;
        the branch segments comprise at least two repeating monomer units and have a number average molecular weight (Mn) of at least about 1,000;
        the branched copolymer component comprises hydrophilic groups; and
        the ratio of component (b) to component (a) is about 1:10 to about 10:1 by weight.
    
    


Another aspect of the present invention is a geotropic propagule coated with an insecticidally effective amount of the aforedescribed composition.
Another aspect of the present invention is a liquid composition consisting of about 5 to 80 weight % of the aforedescribed composition and about 20 to 95 weight % of a volatile aqueous liquid carrier.
Another aspect of the present invention is a method for protecting a geotropic propagule and plant derived therefrom from a phytophagous insect pest, the method comprising coating the propagule with an insecticidally effective amount of the aforedescribed liquid composition and then evaporating the volatile aqueous liquid carrier of the composition.


DETAILED DESCRIPTION
As used herein, the terms “comprises,” “comprising,” “includes,” “including,” “has,” “having,” “contains,” “containing,” “characterized by” or any other variation thereof, are intended to cover a non-exclusive inclusion, subject to any limitation explicitly indicated. For example, a composition, mixture, process or method that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, mixture, process or method.
The transitional phrase “consisting of” excludes any element, step, or ingredient not specified. If in the claim, such would close the claim to the inclusion of materials other than those recited except for impurities ordinarily associated therewith. When the phrase “consisting of” appears in a clause of the body of a claim, rather than immediately following the preamble, it limits only the element set forth in that clause; other elements are not excluded from the claim as a whole.
The transitional phrase “consisting essentially of” is used to define a composition, or method that includes materials, steps, features, components, or elements, in addition to those literally disclosed, provided that these additional materials, steps, features, components, or elements do not materially affect the basic and novel characteristic(s) of the claimed invention. The term “consisting essentially of” occupies a middle ground between “comprising” and “consisting of.”
Where applicants have defined an invention or a portion thereof with an open-ended term such as “comprising,” it should be readily understood that (unless otherwise stated) the description should be interpreted to also describe such an invention using the terms “consisting essentially of” or “consisting of.”
Further, unless expressly stated to the contrary, “or” refers to an inclusive or and not to an exclusive or. For example, a condition A or B is satisfied by any one of the following: A is true (or present) and B is false (or not present), A is false (or not present) and B is true (or present), or both A and B are true (or present).
Also, the indefinite articles “a” and “an” preceding an element or component of the invention are intended to be nonrestrictive regarding the number of instances (i.e., occurrences) of the element or component. Therefore “a” or “an” should be read to include one or at least one, and the singular word form of the element or component also includes the plural unless the number is obviously meant to be singular.
As referred to in the present disclosure and claims, the term “propagule” means a seed or a regenerable plant part. The term “regenerable plant part” means a part of a plant other than a seed from which a whole plant may be grown or regenerated when the plant part is placed in horticultural or agricultural growing media such as moistened soil, peat moss, sand, vermiculite, perlite, rock wool, fiberglass, coconut husk fiber, tree fern fiber and the like, or even a completely liquid medium such as water. The term “geotropic propagule” means a seed or a regenerable plant part obtained from the portion of a plant ordinarily disposed below the surface of the growing medium. Geotropic regenerable plant parts include viable divisions of rhizomes, tubers, bulbs and corms which retain meristematic tissue, such as an eye. Regenerable plant parts such as cut or separated stems and leaves derived from the foliage of a plant are not geotropic and thus are not considered geotropic propagules. As referred to in the present disclosure and claims, unless otherwise indicated, the term “seed” specifically refers to unsprouted seeds. The term “foliage” refers to parts of a plant exposed above ground. Therefore foliage includes leaves, stems, branches, flowers, fruits and buds.
In the context of the present disclosure and claims, protection of a seed or plant grown therefrom from a phytophagous insect pest means protection of the seed or plant from injury or damage potentially caused by the insect pest. This protection is achieved through control of the insect pest. Control of an insect pest can include killing the insect pest, interfering with its growth, development or reproduction, and/or inhibiting its feeding. In the present disclosure and claims the terms “insecticidal” and “insecticidally” relate to any form of insect control.
The terms “suspension concentrate” and “suspension concentrate composition” refer to compositions comprising finely divided solid particles of an active ingredient dispersed in a continuous liquid phase. Said particles retain identity and can be physically separated from the continuous liquid phase. The viscosity of the continuous liquid phase can vary from low to high, and indeed can be so high as to cause the suspension concentrate composition to have a gel-like or paste-like consistency.
The term “particle size” refers to the equivalent spherical diameter of a particle, i.e., the diameter of a sphere enclosing the same volume as the particle. “Median particle size” is the particle size corresponding to half of the particles being larger than the median particle size and half being smaller. With reference to particle size distribution, percentages of particles are also on a volume basis (e.g., “at least 95% of the particles are less than about 10 microns” means that at least 95% of the aggregate volume of particles consists of particles having equivalent spherical diameters of less than about 10 microns). The principles of particle size analysis are well-known to those skilled in the art; for a technical paper providing a summary, see A. Rawle, “Basic Principles of Particle Size Analysis” (document MRK034 published by Malvern Instruments Ltd., Malvern, Worcestershire, UK). Volume distributions of particles in powders can be conveniently measured by such techniques as Low Angle Laser Light Scattering (also known as LALLS and Laser Diffraction), which relies on the fact that diffraction angle is inversely proportional to particle size.
In the recitations herein, the term “alkyl” used either alone or in compound words such as “haloalkyl” or “fluoroalkyl” includes straight-chain or branched alkyl, such as methyl, ethyl, n-propyl, i-propyl, or the different butyl isomers. “Alkoxy” includes, for example, methoxy, ethoxy, n-propyloxy, isopropyloxy and the different butoxy isomers. The term “halogen,” either alone or in compound words such as “haloalkyl,” includes fluorine, chlorine, bromine or iodine. Further, when used in compound words such as “haloalkyl” or “haloalkoxy,” said alkyl may be partially or fully substituted with halogen atoms which may be the same or different. Examples of “haloalkyl” include CF3, CH2Cl, CH2CF3 and CCl2CF3. The terms “haloalkoxy,” and the like, are defined analogously to the term “haloalkyl.” Examples of “haloalkoxy” include OCF3, OCH2CCl3, OCH2CH2CHF2 and OCH2CF3.
The total number of carbon atoms in a substituent group is indicated by the “Ci-Cj” prefix where i and j are numbers from 1 to 4. For example, C1-C4 alkyl designates methyl through butyl, including the various isomers.
The present invention relates to the protection of a geotropic propagule and plant derived therefrom from a phytophagous insect pest by coating the propagule with an insecticidally effective amount of an insecticidal composition comprising by weight based on the total weight of the composition:

    
    
        (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and
        (b) from about 9 to about 91% of a branched copolymer component having a water solubility or dispersibility of at least about 5% by weight at 20° C.,
        wherein:
        the branched copolymer component comprises one or more branch segments attached to a linear backbone segment;
        the branch segments comprise at least two repeating monomer units and have a number average molecular weight (Mn) of at least about 1,000;
        the branched copolymer component comprises hydrophilic groups; and
        the ratio of component (b) to component (a) is about 1:10 to about 10:1 by weight.
    
    


In some embodiments, the inclusion in the composition of present invention of at least about 9% by weight and in a ratio of at least about 1:10 relative to component (a) of a branched copolymer having the above described water solubility or dispersibility, and average molecular weight has been discovered to promote the absorption of the component (a) active ingredient into the propagule when the composition is coated on a propagule either directly or through the emerging roots, thereby providing more uptake of anthranilic diamide insecticides into the developing plant, including emerging foliage. Increasing uptake of anthranilic diamide insecticides provides insecticidally effective concentrations of the insecticides not only in the propagule, roots and foliage near ground level, but also more distant foliage of the growing plant.
Anthranilic diamide insecticides, also known as anthranilamide insecticides, are members of a class of insecticidal compounds characterized chemically by molecular structures comprising vicinal carboxamide substituents bonded to the carbon atoms of an aryl ring, typically phenyl, wherein one carboxamide moiety is bonded through the carbonyl carbon and the other carboxamide moiety is bonded through the nitrogen atom and characterized biologically by binding to ryanodine receptors in insect muscle cells, causing the channel to open and release calcium ions into the cytoplasm. Depletion of calcium ion stores results in insect paralysis and death. PCT Publication WO 2004/027042 describes an assay for ryanodine receptor ligands. Illustrative of anthranilic diamide insecticides are compounds of Formula 1, N-oxides, and salts thereof,




wherein

X is N, CF, CCl, CBr or Cl;
R1 is CH3, Cl, Br or F;
R2 is H, F, Cl, Br or —CN;
R3 is F, Cl, Br, C1-C4 haloalkyl or C1-C4 haloalkoxy;
R4a is H, C1-C4 alkyl, cyclopropylmethyl or 1-cyclopropylethyl;
R4b is H or CH3;
R5 is H, F, Cl or Br; and
R6 is H, F, Cl or Br.
A variety of anthranilic diamide insecticides and methods for their preparation are described in the literature. For example, compounds of Formula 1 and methods for their preparation are reported in U.S. Pat. Nos. 6,747,047 and 7,247,647, and PCT Publications WO 2003/015518, WO 2003/015519, WO 2004/067528, WO2006/062978 and WO2008/069990.
Of particular note for the present compositions and methods of their use are compounds of Formula 1 wherein X is N; R1 is CH3; R2 is Cl or —CN; R3 is Br; R4a is CH3; R4b is H; R5 is H; and R6 is H. The compound wherein R2 is Cl has the Chemical Abstracts systematic name 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide and the common name chlorantraniliprole, and is trademarked as an insecticidal active ingredient by DuPont as RYNAXYPYR. The compound wherein R2 is —CN has the Chemical Abstracts systematic name 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide and the proposed common name cyantraniliprole, and is trademarked as an insecticidal active ingredient by DuPont as CYAZYPYR. As disclosed in Example 15 of WO 2006/062978, cyantraniliprole is in the form of solids melting at 177-181° C. or 217-219° C. Both polymorphs are suitable for the present compositions and methods.
Most generally, component (a) is from about 9 to about 91% of the composition by weight. Typically component (a) is at least about 20%, more typically at least about 30%, and most typically at least 40% of the composition by weight. Component (a) is typically not more than about 80% and more typically not more than about 70% of the composition by weight. To provide optimal biological availability, typically not more than about 30% of component (a), more typically not more than about 20%, and most typically not more than about 10% of component (a) by weight is present in the composition as particles having a particle size greater than about 10 microns. Particle sizes of 10 microns or less can be easily achieved through such techniques as milling.
The branched copolymer component comprises one or more branch segments attached to a linear backbone segment. The branched copolymer has a number average molecular weight (Mn) of about 2,000-500,000. The weight ratio of the linear backbone segment to the branch segments is generally between about 50/1 to about 1/10, or between about 80/20 to about 60/40. In some embodiments, the branched copolymer component contains at least 0.5% by weight of branch segments. The branch segments, also known as polymer arms, typically are randomly distributed along the linear backbone segment. Each branch segment is a polymer, copolymer, or oligomer of at least two repeating monomer units, which may be the same or different, and is attached to the linear backbone segment by a covalent bond.
The branch segments can be incorporated into the branched copolymer component by addition polymerization of a macromer and a comonomer. Typically, the macromers comprise 0.5-80%, or 5-50% of the combined weight of the comonomers and macromers during a free radical polymerization process. Alternatively, the branched copolymer component can be prepared by any known method of preparing branched polymers using either addition or condensation reactions. Alternative processes include the use of preformed backbones and branch segments, or in situ polymerized segments.
Typically, the comonomer contains a single ethylenic group that can copolymerize with the macromer.
A “macromer” for the purpose of this invention, is a polymer, copolymer, or oligomer with an Mn from at least about 1,000 to about 40,000, or 1,000-15,000, or 1,000-8,000, and contains a terminal, ethylenically unsaturated, polymerizable group. In some embodiments, the macromer is a linear polymer or copolymer, end-capped with an ethylenic group (—CH═CH2). Typically, the macromer comprises from about 2-500 monomer units, or 30-200 monomer units.
The macromer, the branch segment derived from it, and/or the linear backbone of the branched copolymer component comprise one or more hydrophilic groups. As used herein, a “hydrophilic group” is a functional group that enhances the solubility, swellability, or dispersibility in aqueous environments of the macromer and/or the branched copolymer component. Suitable macromers can be prepared according to the general description in U.S. Pat. No. 4,680,352 and U.S. Pat. No. 4,694,054. Macromers can be prepared by free radical polymerization processes employing a cobalt compound as a catalytic chain transfer agent, particularly a cobalt(II) compound. Low molecular weight methacrylate macromers can also be prepared using a pentacyanocobalt(II) catalytic chain transfer agent, as disclosed in U.S. Pat. No. 4,722,984.
Suitable macromers include methacrylate polymers derived from acrylates or other vinyl monomers. More specifically, suitable monomers for use in preparing macromers include: tert-butyl methacrylate (tBMA); tert-butyl acrylate (tBA); methyl methacrylate (MMA); ethyl methacrylate (EMA); butyl methacrylate (BMA); 2-ethylhexyl methacrylate (EHMA); methyl acrylate (MA); ethyl acrylate (EA); butyl acrylate (BA); 2-ethylhexyl acrylate (EHA); 2-hydroxyethyl methacrylate (HEMA); 2-hydroxyethyl acrylate (HEA); methacrylic acid (MAA); acrylic acid (AA); hydroxypropyl methacrylate (HPMA); polypropyleneglycol (5) methacrylate (PPM5); hydroxypropyl acrylate (HPA); polypropyleneglycol (5) acrylate (PPA5); esters of acrylic and methacrylic acid wherein the ester group contains from 1 to 18 carbon atoms; amides of acrylic and methacrylic acid (e.g., acrylamide); acrylonitrile; glycidyl methacrylate; glycidyl acrylate; itaconic acid (IA) and itaconic acid anhydride (ITA), half esters and imides; maleic acid and maleic acid anhydride, half esters and imides; aminoethyl methacylate; tert-butyl aminoethyl methacrylate; dimethyl aminoethyl methacrylate; diethyl aminoethyl methacrylate; aminoethyl acrylate; dimethyl aminoethyl arylate; diethylaminoethyl acrylate; N-tert-octyl acrylamide; vinyl methyl ether; styrene (STY); alpha-methyl styrene (AMS); vinyl acetate; vinyl chloride; and mixtures thereof.
Suitable comonomers include acrylates, methacrylates, styrenics, and mixtures thereof. More specifically, suitable comonomers include: tert-butyl methacrylate (tBMA); tert-butyl acrylate (tBA); methyl methacrylate (MMA); ethyl methacrylate (EMA); butyl methacrylate (BMA); 2-ethylhexyl methacrylate (EHMA); methyl acrylate (MA); ethyl acrylate (EA); butyl acrylate (BA); 2-ethylhexyl acrylate; 2-hydroxyethyl methacrylate (HEMA); 2-hydroxyethyl acrylate (HEA); methacrylic acid (MAA); acrylic acid (AA); hydroxypropyl methacrylate (HPMA); polypropyleneglycol (5) methacrylate (PPM5); hydroxypropyl acrylate (HPA); polypropyleneglycol (5) acrylate (PPA5); acrylonitrile (AN); methacrylonitrile (MAN); itaconic acid (IA) and itaconic acid anhydride (ITA), half esters, and imides; maleic acid and maleic acid anhydride, half esters, and imides; aminoethyl methacrylate; tert-butyl aminoethyl methacrylate; dimethyl aminoethyl methacrylate; diethyl aminoethyl methacrylate; aminoethyl acrylate; dimethyl aminoethyl acrylate; diethyl aminoethyl acrylate; acrylamide; N-tert-octyl acrylamide; vinyl methyl ethers; styrene (STY); alpha-methyl styrene; vinyl acetate; vinyl chloride; and mixture thereof. At least 50% of the comonomers should be selected from (meth)acrylate monomers, styrenic monomers, or mixtures thereof.
The linear backbone segment and/or the branch segments of the branched copolymer component can comprise one or more functional groups. A “functional group” is any moiety capable of being attached to the linear backbone segment or a branch segment by a direct valence bond or by a linking group.
Suitable functional groups include:
—SO3H, and —CN;
—COOR, —OR, and —SR,


    
    
        where R is H, C1-C12 alkyl, C3-C12 cycloalkyl, aryl, aralkyl, aralkyl, a heterocyclic group, or —OR1, where R1 is C1-C12 alkyl, aryl, aralkyl or aralkyl;

—NR2R3 and

    
    






    
    
        where R2 and R3 are independently H, C1-C12 alkyl, C3-C12 cycloalkyl, aryl, alkaryl, aralkyl, or —CH2OR4, where R4 is H, C1-C12 alkyl, C3-C12 cycloalkyl, aryl, alkaryl, aralkyl, or together R2 and R3 form a heterocyclic ring;
    
    






    
    
        where R5, R6 and R7 are independently H, C1-C12 alkyl, C3-C12 cycloalkyl, aryl, alkaryl, aralkyl, or —COOR, or when taken together, R5, R6 and/or R7 form a cyclic group;

—SiR8R9R10,

        where R8, R9 and R10 are independently C1-C12 alkyl, C3-C12 cycloalkyl or —OR11, where R11 is C1-C12 alkyl, C3-C12 cycloalkyl, aryl, alkaryl or aralkyl;

—OSO3R12, —OPO2R12, —PO2R12, —PR12R13R14, —OPOR12, —SR12R13, and —N+R12R13R14,

        where R12, R13 and R14 are independently H, C1-C12 alkyl, C3-C12 cycloalkyl, aryl, alkaryl or aralkyl; and

salts thereof.

    
    


Suitable functional groups also include: urethane, isocyanate, blocked isocyanate, urea, oxirane, aziridine, quinine diazide, azo, azide, diazonium, and acetylacetoxy groups, and salts thereof.
In some embodiments, the branched copolymer component comprises one or more of the following functional groups: —COOH, —OH, —NH2, an amide group, a vinyl group, a urethane group, an isocyanate group, a blocked isocyanate group. Functional groups can be located anywhere on the branched polymer.
In some embodiments, macromers have a molecular weight (Mn) in the range of 1,000-8,000, or 1,000-5,000, and branched copolymers have a molecular weight (Mn) range of 2,000-50,000, or 2,000-30,000.
The branched copolymer component can also be produced by forming a macromer according to the macromonomer polymerization process described in Example 2 in World Patent Publication WO1995/032228. The macromonomer can then be polymerized in the presence of backbone monomers mixture according to the general process of Example 10 described in the aforementioned patent publication to form the branched copolymer component. Alternately, the macromonomer polymerization process described in Example 1 of U.S. Pat. No. 5,231,131 can be used to form the macromer. The macromonomer can then be polymerized in the presence of backbone monomers mixture, according to the general process of Example 2 described in U.S. Pat. No. 5,231,131.
Generally, increasing the weight ratio of component (b) to component (a) increases the absorption of component (a) into the propagule and/or developing roots to protect also the foliage of a plant grown from a propagule coated with a composition comprising components (a) and (b). However, increasing component (b) also reduces the amount of component (a) that can be included in the composition. Generally, the weight ratio of component (b) to component (a) is at least about 1:10, typically at least about 1:8, more typically from at least about 1:5 or 1:4, and most typically at least about 1:3. In some embodiments the weight ratio of component (a) to component (b) is at least about 1:2 or 1:1. Generally, the weight ratio of component (b) to component (a) is not more than about 10:1, typically not more than about 8:1, more typically not more than about 4:1, and most typically not more than about 3:1. In some embodiments the weight ratio of component (a) to component (b) is not more than about 2:1 or 1:1.
Most generally, component (b) is from about 9 to about 91% of the composition by weight. Increasing the amount of component (b) can increase the ratio of component (b) to component (a) to facilitate absorption of component (a) from the propagule coating into the propagule and/or developing roots, but also reduces the concentration of component (a) in the coating and accordingly requires a thicker coating to provide a desired amount of component (a) for each propagule. Typically component (b) is at least about 15%, more typically at least about 20%, and most typically at least 25% of the composition by weight. In some embodiments, component (b) is at least about 30%, 35% or 40% of the composition by weight. Component (b) is typically not more than about 80%, more typically not more than about 70%, and most typically not more than about 60% of the composition by weight. In some embodiments, component (b) is not more than about 50% or 40% of the composition by weight.
The present composition can optionally further comprise (c) up to about 90% by weight of one or more biologically active agents other than anthranilic diamide insecticides. Biologically active agents of component (c) do not include biocides whose principal effect is to preserve the present composition rather than protect a plant contacted with the present composition.
If present, component (c) is typically at least about 0.1% and more typically at least about 1% of the composition by weight. Typically component (c) is not more than about 60%, more typically not more than about 50%, 40% or 30%, and most typically not more than about 20% of the composition by weight. The biologically active agents forming component (c) differ from the component (a) anthranilic diamide insecticides and can include chemical compounds or biological organisms selected from the following classes: insecticides, fungicides, nematocides, bactericides, acaricides, herbicides, growth regulators such as rooting stimulants, chemosterilants, semiochemicals, repellents, attractants, pheromones and feeding stimulants (including both chemical and biological agents), and mixtures of several compounds or organisms selected from the above classes.
Compositions comprising different biologically active agents can have a broader spectrum of activity than a single agent alone. Furthermore, such mixtures can exhibit a synergistic effect.
Examples of component (c) (i.e., the one or more biologically active agents other than anthranilic diamide insecticides) include: insecticides such as abamectin, acephate, acequinocyl, acetamiprid, acrinathrin, amidoflumet, amitraz, avermectin, azadirachtin, azinphos-methyl, bifenthrin, bifenazate, bistrifluoron, borate, buprofezin, cadusafos, carbaryl, carbofuran, cartap, carzol, chlorantraniliprole, chlorfenapyr, chlorfluazuron, chlorpyrifos, chlorpyrifos-methyl, chromafenozide, clofentezin, clothianidin, cyantraniliprole, cyflumetofen, cyfluthrin, beta-cyfluthrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, zeta-cypermethrin, cyromazine, deltamethrin, diafenthiuron, diazinon, dieldrin, diflubenzuron, dimefluthrin, dimehypo, dimethoate, dinotefuran, diofenolan, emamectin, endosulfan, esfenvalerate, ethiprole, etofenprox, etoxazole, fenbutatin oxide, fenothiocarb, fenoxycarb, fenpropathrin, fenvalerate, fipronil, flonicamid, flubendiamide, flucythrinate, flufenerim, flufenoxuron, fluvalinate, tau-fluvalinate, fonophos, formetanate, fosthiazate, halofenozide, hexaflumuron, hexythiazox, hydramethylnon, imidacloprid, indoxacarb, insecticidal soaps, isofenphos, lufenuron, malathion, metaflumizone, metaldehyde, methamidophos, methidathion, methiodicarb, methomyl, methoprene, methoxychlor, metofluthrin, monocrotophos, methoxyfenozide, nitenpyram, nithiazine, novaluron, noviflumuron, oxamyl, parathion, parathion-methyl, permethrin, phorate, phosalone, phosmet, phosphamidon, pirimicarb, profenofos, profluthrin, propargite, protrifenbute, pymetrozine, pyrafluprole, pyrethrin, pyridaben, pyridalyl, pyrifluquinazon, pyriprole, pyriproxyfen, rotenone, ryanodine, spinetoram, spinosad, spirodiclofen, spiromesifen, spirotetramat, sulprofos, tebufenozide, tebufenpyrad, teflubenzuron, tefluthrin, terbufos, tetrachlorvinphos, tetramethrin, thiacloprid, thiamethoxam, thiodicarb, thiosultap-sodium, tolfenpyrad, tralomethrin, triazamate, trichlorfon, triflumuron, Bacillus thuringiensis delta-endotoxins, entomopathogenic bacteria, entomopathogenic viruses and entomopathogenic fungi.
Of note are insecticides such as abamectin, acetamiprid, acrinathrin, amitraz, avermectin, azadirachtin, bifenthrin, buprofezin, cadusafos, carbaryl, cartap, chlorantraniliprole, chlorfenapyr, chlorpyrifos, clothianidin, cyantraniliprole, cyfluthrin, beta-cyfluthrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, zeta-cypermethrin, cyromazine, deltamethrin, dieldrin, dinotefuran, diofenolan, emamectin, endosulfan, esfenvalerate, ethiprole, etofenprox, etoxazole, fenothiocarb, fenoxycarb, fenvalerate, fipronil, flonicamid, flubendiamide, flufenoxuron, fluvalinate, formetanate, fosthiazate, hexaflumuron, hydramethylnon, imidacloprid, indoxacarb, lufenuron, metaflumizone, methiodicarb, methomyl, methoprene, methoxyfenozide, nitenpyram, nithiazine, novaluron, oxamyl, pymetrozine, pyrethrin, pyridaben, pyridalyl, pyriproxyfen, ryanodine, spinetoram, spinosad, spirodiclofen, spiromesifen, spirotetramat, tebufenozide, tetramethrin, thiacloprid, thiamethoxam, thiodicarb, thiosultap-sodium, tralomethrin, triazamate, triflumuron, Bacillus thuringiensis delta-endotoxins, all strains of Bacillus thuringiensis and all strains of Nucleo polyhydrosis viruses.
One embodiment of biological agents for mixing with compounds of this invention include entomopathogenic bacteria such as Bacillus thuringiensis, and the encapsulated delta-endotoxins of Bacillus thuringiensis such as MVP® and MVPII® bioinsecticides prepared by the CellCap® process (CelICap®, MVP® and MVPII® are trademarks of Mycogen Corporation, Indianapolis, Ind., USA); entomopathogenic fungi such as green muscardine fungus; and entomopathogenic (both naturally occurring and genetically modified) viruses including baculovirus, nucleopolyhedro virus (NPV) such as Helicoverpa zea nucleopolyhedrovirus (HzNPV), Anagrapha falcifera nucleopolyhedrovirus (AfNPV); and granulosis virus (GV) such as Cydia pomonella granulosis virus (CpGV).
Of particular note is such a combination where the other biologically active agent belongs to a different chemical class or has a different site of action than the compound of Formula 1. In certain instances, a combination with at least one other biologically active agent having a similar spectrum of control but a different site of action will be particularly advantageous for resistance management. Thus, a composition of the present invention can further comprise at least one additional biologically active agent having a similar spectrum of control but belonging to a different chemical class or having a different site of action. These additional biologically active compounds or agents include, but are not limited to, sodium channel modulators such as bifenthrin, cypermethrin, cyhalothrin, lambda-cyhalothrin, cyfluthrin, beta-cyfluthrin, deltamethrin, dimefluthrin, esfenvalerate, fenvalerate, indoxacarb, metofluthrin, profluthrin, pyrethrin and tralomethrin; cholinesterase inhibitors such as chlorpyrifos, methomyl, oxamyl, thiodicarb and triazamate; neonicotinoids such as acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, nithiazine, thiacloprid and thiamethoxam; insecticidal macrocyclic lactones such as spinetoram, spinosad, abamectin, avermectin and emamectin; GABA (gamma-aminobutyric acid)-gated chloride channel antagonists such as avermectin or blockers such as ethiprole and fipronil; chitin synthesis inhibitors such as buprofezin, cyromazine, flufenoxuron, hexaflumuron, lufenuron, novaluron, noviflumuron and triflumuron; juvenile hormone mimics such as diofenolan, fenoxycarb, methoprene and pyriproxyfen; octopamine receptor ligands such as amitraz; molting inhibitors and ecdysone agonists such as azadirachtin, methoxyfenozide and tebufenozide; ryanodine receptor ligands such as ryanodine, anthranilic diamides such as chlorantraniliprole, cyantraniliprole and flubendiamide; nereistoxin analogs such as cartap; mitochondrial electron transport inhibitors such as chlorfenapyr, hydramethylnon and pyridaben; lipid biosynthesis inhibitors such as spirodiclofen and spiromesifen; cyclodiene insecticides such as dieldrin or endosulfan; pyrethroids; carbamates; insecticidal ureas; and biological agents including nucleopolyhedro viruses (NPV), members of Bacillus thuringiensis, encapsulated delta-endotoxins of Bacillus thuringiensis, and other naturally occurring or genetically modified insecticidal viruses.
Further examples of biologically active compounds or agents with which compounds of this invention can be formulated are: fungicides such as acibenzolar, aldimorph, amisulbrom, azaconazole, azoxystrobin, benalaxyl, benomyl, benthiavalicarb, benthiavalicarb-isopropyl, binomial, biphenyl, bitertanol, blasticidin-S, Bordeaux mixture (Tribasic copper sulfate), boscalid/nicobifen, bromuconazole, bupirimate, buthiobate, carboxin, carpropamid, captafol, captan, carbendazim, chloroneb, chlorothalonil, chlozolinate, clotrimazole, copper oxychloride, copper salts such as copper sulfate and copper hydroxide, cyazofamid, cyflunamid, cymoxanil, cyproconazole, cyprodinil, dichlofluanid, diclocymet, diclomezine, dicloran, diethofencarb, difenoconazole, dimethomorph, dimoxystrobin, diniconazole, diniconazole-M, dinocap, discostrobin, dithianon, dodemorph, dodine, econazole, etaconazole, edifenphos, epoxiconazole, ethaboxam, ethirimol, ethridiazole, famoxadone, fenamidone, fenarimol, fenbuconazole, fencaramid, fenfuram, fenhexamide, fenoxanil, fenpiclonil, fenpropidin, fenpropimorph, fentin acetate, fentin hydroxide, ferbam, ferfurazoate, ferimzone, fluazinam, fludioxonil, flumetover, fluopicolide, fluoxastrobin, fluquinconazole, fluquinconazole, flusilazole, flusulfamide, flutolanil, flutriafol, folpet, fosetyl-aluminum, fuberidazole, furalaxyl, furametapyr, hexaconazole, hymexazole, guazatine, imazalil, imibenconazole, iminoctadine, iodicarb, ipconazole, iprobenfos, iprodione, iprovalicarb, isoconazole, isoprothiolane, kasugamycin, kresoxim-methyl, mancozeb, mandipropamid, maneb, mapanipyrin, mefenoxam, mepronil, metalaxyl, metconazole, methasulfocarb, metiram, metominostrobin/fenominostrobin, mepanipyrim, metrafenone, miconazole, myclobutanil, neo-asozin (ferric methanearsonate), nuarimol, octhilinone, ofurace, orysastrobin, oxadixyl, oxolinic acid, oxpoconazole, oxycarboxin, paclobutrazol, penconazole, pencycuron, penthiopyrad, perfurazoate, phosphonic acid, phthalide, picobenzamid, picoxystrobin, polyoxin, probenazole, prochloraz, procymidone, propamocarb, propamocarb-hydrochloride, propiconazole, propineb, proquinazid, prothioconazole, pyraclostrobin, pryazophos, pyrifenox, pyrimethanil, pyrifenox, pyrolnitrine, pyroquilon, quinconazole, quinoxyfen, quintozene, silthiofam, simeconazole, spiroxamine, streptomycin, sulfur, tebuconazole, techrazene, tecloftalam, tecnazene, tetraconazole, thiabendazole, thifluzamide, thiophanate, thiophanate-methyl, thiram, tiadinil, tolclofos-methyl, tolyfluanid, triadimefon, triadimenol, triarimol, triazoxide, tridemorph, trimoprhamide tricyclazole, trifloxystrobin, triforine, triticonazole, uniconazole, validamycin, vinclozolin, zineb, ziram, and zoxamide; nematocides such as aldicarb, imicyafos, oxamyl and fenamiphos; bactericides such as streptomycin; acaricides such as amitraz, chinomethionat, chlorobenzilate, cyhexatin, dicofol, dienochlor, etoxazole, fenazaquin, fenbutatin oxide, fenpropathrin, fenpyroximate, hexythiazox, propargite, pyridaben and tebufenpyrad.
In certain instances, combinations of a compound of this invention with other biologically active (particularly invertebrate pest control) compounds or agents (i.e. active ingredients) can result in a greater-than-additive (i.e., synergistic) effect. Reducing the quantity of active ingredients released in the environment while ensuring effective pest control is always desirable. When synergism with biologically active agents occurs at application rates giving agronomically satisfactory levels of insect control, such combinations can be advantageous for reducing crop production cost and decreasing environmental load.
Compounds of this invention and compositions thereof can be applied to plants genetically transformed to express proteins toxic to insect pests (such as Bacillus thuringiensis delta-endotoxins). Such an application may provide a broader spectrum of plant protection and be advantageous for resistance management. The effect of the exogenously applied compounds of this invention may be synergistic with the expressed toxin proteins.
General references for these agricultural protectants (i.e., insecticides, fungicides, nematocides, acaricides, herbicides and biological agents) include The Pesticide Manual, 13th Edition, C. D. S. Tomlin, Ed., British Crop Protection Council, Farnham, Surrey, U.K., 2003 and The BioPesticide Manual, 2nd Edition, L. G. Copping, Ed., British Crop Protection Council, Farnham, Surrey, U.K., 2001.
Table A lists specific combinations of a compound of Formula 1 with other biologically active agents illustrative of the mixtures, compositions and methods of the present invention and includes additional embodiments of weight ratio ranges for application rates. The first column of Table A lists the specific insect control agents (e.g., “Abamectin” in the first line). The second column of Table A lists the mode of action (if known) or chemical class of the insect pest control agents. The third column of Table A lists embodiment(s) of ranges of weight ratios for rates at which the insect pest control agent can be applied relative to a compound of Formula 1 (e.g., “50:1 to 1:50” of abamectin relative to a compound of Formula 1 by weight). Thus, for example, the first line of Table A specifically discloses the combination of a compound of Formula 1 with abamectin can be applied in a weight ratio between 50:1 to 1:50. The remaining lines of Table A are to be construed similarly.









TABLE A





Insect Pest Control
Mode of Action or Chemical
Typical


Agent
Class
Weight Ratio







Abamectin
macrocyclic lactones
50:1 to 1:50


Acetamiprid
neonicotinoids
150:1 to 1:200


Amitraz
octopamine receptor ligands
200:1 to 1:100


Avermectin
macrocyclic lactones
50:1 to 1:50


Azadirachtin
ecdysone agonists
100:1 to 1:120


Beta-cyfluthrin
sodium channel modulators
150:1 to 1:200


Bifenthrin
sodium channel modulators
100:1 to 1:10 


Buprofezin
chitin synthesis inhibitors
500:1 to 1:50 


Cartap
nereistoxin analogs
100:1 to 1:200


Chlorantraniliprole
ryanodine receptor ligands
100:1 to 1:120


Chlorfenapyr
mitochondrial electron transport
300:1 to 1:200



inhibitors



Chlorpyrifos
cholinesterase inhibitors
500:1 to 1:200


Clothianidin
neonicotinoids
100:1 to 1:400


Cyantraniliprole
ryanodine receptor ligands
100:1 to 1:120


Cyfluthrin
sodium channel modulators
150:1 to 1:200


Cyhalothrin
sodium channel modulators
150:1 to 1:200


Cypermethrin
sodium channel modulators
150:1 to 1:200


Cyromazine
chitin synthesis inhibitors
400:1 to 1:50 


Deltamethrin
sodium channel modulators
 50:1 to 1:400


Dieldrin
cyclodiene insecticides
200:1 to 1:100


Dinotefuran
neonicotinoids
150:1 to 1:200


Diofenolan
molting inhibitor
150:1 to 1:200


Emamectin
macrocyclic lactones
50:1 to 1:10


Endosulfan
cyclodiene insecticides
200:1 to 1:100


Esfenvalerate
sodium channel modulators
100:1 to 1:400


Ethiprole
GABA-regulated chloride
200:1 to 1:100



channel blockers



Fenothiocarb

150:1 to 1:200


Fenoxycarb
juvenile hormone mimics
500:1 to 1:100


Fenvalerate
sodium channel modulators
150:1 to 1:200


Fipronil
GABA-regulated chloride
150:1 to 1:100



channel blockers



Flonicamid

200:1 to 1:100


Flubendiamide
ryanodine receptor ligands
100:1 to 1:120


Flufenoxuron
chitin synthesis inhibitors
200:1 to 1:100


Hexaflumuron
chitin synthesis inhibitors
300:1 to 1:50 


Hydramethylnon
mitochondrial electron transport
150:1 to 1:250



inhibitors



Imidacloprid
neonicotinoids
1000:1 to 1:1000


Indoxacarb
sodium channel modulators
200:1 to 1:50 


Lambda-
sodium channel modulators
 50:1 to 1:250


cyhalothrin




Lufenuron
chitin synthesis inhibitors
500:1 to 1:250


Metaflumizone

200:1 to 1:200


Methomyl
cholinesterase inhibitors
500:1 to 1:100


Methoprene
juvenile hormone mimics
500:1 to 1:100


Methoxyfenozide
ecdysone agonists
50:1 to 1:50


Nitenpyram
neonicotinoids
150:1 to 1:200


Nithiazine
neonicotinoids
150:1 to 1:200


Novaluron
chitin synthesis inhibitors
500:1 to 1:150


Oxamyl
cholinesterase inhibitors
200:1 to 1:200


Pymetrozine

200:1 to 1:100


Pyrethrin
sodium channel modulators
100:1 to 1:10 


Pyridaben
mitochondrial electron transport
200:1 to 1:100



inhibitors



Pyridalyl

200:1 to 1:100


Pyriproxyfen
juvenile hormone mimics
500:1 to 1:100


Ryanodine
ryanodine receptor ligands
100:1 to 1:120


Spinetoram
macrocyclic lactones
150:1 to 1:100


Spinosad
macrocyclic lactones
500:1 to 1:10 


Spirodiclofen
lipid biosynthesis inhibitors
200:1 to 1:200


Spiromesifen
lipid biosynthesis inhibitors
200:1 to 1:200


Tebufenozide
ecdysone agonists
500:1 to 1:250


Thiacloprid
neonicotinoids
100:1 to 1:200


Thiamethoxam
neonicotinoids
1250:1 to 1:1000


Thiodicarb
cholinesterase inhibitors
500:1 to 1:400


Thiosultap-sodium

150:1 to 1:100


Tralomethrin
sodium channel modulators
150:1 to 1:200


Triazamate
cholinesterase inhibitors
250:1 to 1:100


Triflumuron
chitin synthesis inhibitors
200:1 to 1:100


Bacillus
biological agents
50:1 to 1:10


thuringiensis




Bacillus
biological agents
50:1 to 1:10


thuringiensis delta-




endotoxin




NPV (e.g.,
biological agents
50:1 to 1:10


Gemstar)









Of note is the composition of the present invention wherein the at least one additional biologically active compound or agent is selected from the insect pest control agents listed in Table A above.
The weight ratios of a compound of Formula 1, an N-oxide, or a salt thereof, to the additional insect pest control agent typically are between 1000:1 and 1:1000, with one embodiment being between 500:1 and 1:500, another embodiment being between 250:1 and 1:200 and another embodiment being between 100:1 and 1:50.
Listed below in Tables B1 and B2 are embodiments of specific compositions comprising a compound of Formula 1 (Compound 1 is 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)-carbonyl]phenyl]-1H-pyrazole-5-carboxamide and Compound 2 is 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide) and an additional insect pest control agent.












TABLE B1







Mixture
Cmpd.

Invertebrate Pest



No.
No.
and
Control Agent





















B1-1
1
and
Abamectin



B1-2
1
and
Acetamiprid



B1-3
1
and
Amitraz



B1-4
1
and
Avermectin



B1-5
1
and
Azadirachtin



B1-5a
1
and
Bensultap



B1-6
1
and
Beta-cyfluthrin



B1-7
1
and
Bifenthrin



B1-8
1
and
Buprofezin



B1-9
1
and
Cartap



B1-10
1
and
Chlorantraniliprole



B1-11
1
and
Chlorfenapyr



B1-12
1
and
Chlorpyrifos



B1-13
1
and
Clothianidin



B1-14
1
and
Cyantraniliprole



B1-15
1
and
Cyfluthrin



B1-16
1
and
Cyhalothrin



B1-17
1
and
Cypermethrin



B1-18
1
and
Cyromazine



B1-19
1
and
Deltamethrin



B1-20
1
and
Dieldrin



B1-21
1
and
Dinotefuran



B1-22
1
and
Diofenolan



B1-23
1
and
Emamectin



B1-24
1
and
Endosulfan



B1-25
1
and
Esfenvalerate



B1-26
1
and
Ethiprole



B1-27
1
and
Fenothiocarb



B1-28
1
and
Fenoxycarb



B1-29
1
and
Fenvalerate



B1-30
1
and
Fipronil



B1-31
1
and
Flonicamid



B1-32
1
and
Flubendiamide



B1-33
11
and
Flufenoxuron



B1-34
1
and
Hexaflumuron



B1-35
1
and
Hydramethylnon



B1-36
1
and
Imidacloprid



B1-37
1
and
Indoxacarb



B1-38
1
and
Lambda-






cyhalothrin



B1-39
1
and
Lufenuron



B1-40
1
and
Metaflumizone



B1-41
1
and
Methomyl



B1-42
1
and
Methoprene



B1-43
1
and
Methoxyfenozide



B1-44
1
and
Nitenpyram



B1-45
1
and
Nithiazine



B1-46
1
and
Novaluron



B1-47
1
and
Oxamyl



B1-48
1
and
Phosmet



B1-49
1
and
Pymetrozine



B1-50
1
and
Pyrethrin



B1-51
1
and
Pyridaben



B1-52
1
and
Pyridalyl



B1-53
1
and
Pyriproxyfen



B1-54
1
and
Ryanodine



B1-55
1
and
Spinetoram



B1-56
1
and
Spinosad



B1-57
1
and
Spirodiclofen



B1-58
1
and
Spiromesifen



B1-59
1
and
Spirotetramat



B1-60
1
and
Tebufenozide



B1-61
1
and
Thiacloprid



B1-62
1
and
Thiamethoxam



B1-63
1
and
Thiodicarb



B1-64
1
and
Thiosultap-






sodium



B1-65
1
and
Tolfenpyrad



B1-66
1
and
Tralomethrin



B1-67
1
and
Triazamate



B1-68
1
and
Triflumuron



B1-69
1
and
Bacillus






thuringiensis



B1-70
1
and
Bacillus






thuringiensis






delta-endotoxin



B1-71
1
and
NPV (e.g.,






Gemstar)










Table B2
Table B2 is identical to Table B1, except that each reference to Compound 1 in the column headed “Cmpd. No.” is replaced by a reference to Compound 2. For example, the first mixture in Table B2 is designated B2-1 and is a mixture of Compound 2 and the additional insect pest control agent abamectin.
The specific mixtures listed in Tables B1 and B2 typically combine a compound of Formula 1 with the other invertebrate pest agent in the ratios specified in Table A.
Listed below in Tables C1 and C2 are embodiments of specific compositions comprising a compound of Formula 1 (Compound 1 is 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)-carbonyl]phenyl]-1H-pyrazole-5-carboxamide and Compound 2 is 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide) and an additional fungicide.












TABLE C1







Mixture
Cmpd.





No.
No.
and
Fungicide





















C1-1
1
and
Probenazole



C1-2
1
and
Tiadinil



C1-3
1
and
Isotianil



C1-4
1
and
Pyroquilon



C1-5
1
and
Metominostrobin



C1-6
1
and
Flutolanil



C1-7
1
and
Validamycin



C1-8
1
and
Furametpyr



C1-9
1
and
Pencycuron



C1-10
1
and
Simeconazole



C1-11
1
and
Orysastrobin



C1-12
1
and
Trifloxystrobin



C1-13
1
and
Isoprothiolane



C1-14
1
and
Azoxystrobin



C1-15
1
and
Tricyclazole



C1-16
1
and
Hexaconazole



C1-17
1
and
Difenoconazole



C1-18
1
and
Cyproconazole



C1-19
1
and
Propiconazole



C1-20
1
and
Fenoxanil



C1-21
1
and
Ferimzone



C1-22
1
and
Fthalide



C1-23
1
and
Kasugamycin



C1-24
1
and
Picoxystrobin



C1-25
1
and
Penthiopyrad



C1-26
1
and
Famoxadone



C1-27
1
and
Cymoxanil



C1-28
1
and
Proquinazid



C1-29
1
and
Flusilazole



C1-30
1
and
Mancozeb



C1-31
1
and
Copper






hydroxide



C1-32
1
and
(a)







(a) 1-[4-[4-[5-(2,6-difluorophenyl)-4,5-dihydro-3-isoxazolyl]-2-thiazolyl]-1-piperidinyl]-2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl]ethanone






Table C2
Table C2 is identical to Table C1, except that each reference to Compound 1 in the column headed “Cmpd. No.” is replaced by a reference to Compound 2. For example, the first mixture in Table C2 is designated C2-1 and is a mixture of Compound 2 and the additional fungicide probenazole.
As an alternative to including other biologically active agents as component (c) in the present composition, other biologically active ingredients can be separately applied to propagules.
The present composition can optionally further comprise (d) up to about 80% by weight of one or more inert formulating ingredients other than branched copolymer of component (b). As used herein, the term “inert formulating ingredient” refers to ingredients included in compositions other than the chemicals or other agents providing the biological activity to control the intended pests (e.g., as described for component (c)). Such inert formulating ingredients are also known as formulation aids. When present, component (d) is typically at least 0.1% of the composition by weight. Except when the composition is intended for pelleting seeds, the amount of component (d) is typically not more than about 20% of the composition by weight.
Component (d) can comprise a wide variety of inert formulating ingredients other than the branched copolymers of component (b), including for example, adhesives, liquid diluents, solid diluents, surfactants (e.g., having wetting agent, dispersant and/or anti-foam properties), antifreeze agents, preservatives such as chemical stabilizers or biocides, thickening agents and fertilizers. The branched copolymers of component (b) can function as surfactants (e.g., wetting agents, dispersants) and/or adhesives. Indeed, acrylate/methacrylate-based branched copolymers are well-known for their wetting and dispersing properties, although they are generally included in formulations at concentrations substantially less than specified herein. Therefore component (b) can reduce or eliminate the benefit of including certain additional inert formulating ingredients as constituents of component (d). Nevertheless, inclusion of ingredients such as surfactants and adhesives in component (d) may still be desirable.
In the context of the present disclosure and claims, the term “adhesive” refers to a substance capable of binding component (a) to a propagule such as a seed. Adhesives include substances exhibiting tackiness such as methylcellulose or gum arabic, which are known as sticking agents. Adhesives also include substances known as film-formers, which provide a durable uniform film when applied to a surface. Although an adhesive substance can be included as a constituent of component (d) in the present composition, such inclusion is often not advantageous, because the branched copolymers of component (b) have adhesive properties. However, including additional adhesive substance is most likely to be advantageous when component (b) is a liquid or paste (i.e., not solid), and particularly when component (b) is a liquid.
The adhesive agent can comprise an adhesive polymer that is natural or synthetic and is without phytotoxic effect on the seed or propagule to be coated. The adhesive agent can be selected from the group consisting of polyvinyl acetates, polyvinyl acetate copolymers, hydrolyzed polyvinyl acetates, polyvinylpyrrolidone-vinyl acetate copolymers, polyvinyl alcohols, polyvinyl alcohol copolymers, polyvinyl methyl ether, polyvinyl methyl ether-maleic anhydride copolymers, waxes, latex polymers, celluloses including ethylcelluloses and methylcelluloses, hydroxymethylcelluloses, hydroxypropylcelluloses, hydroxymethylpropyl-celluloses, polyvinylpyrrolidones, alginates, dextrins, malto-dextrins, polysaccharides, fats, oils, proteins, karaya gum, jaguar gum, tragacanth gum, polysaccharide gums, mucilage, gum arabics, shellacs, vinylidene chloride polymers and copolymers, soybean protein-based polymers and copolymers, lignosulfonates, acrylic copolymers, starches, polyvinylacrylates, zeins, gelatin, carboxymethylcellulose, chitosan, polyethylene oxide, acrylimide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylimide polymers, alginate, ethylcellulose, polychloroprene, and syrups or mixtures thereof. The above-identified polymers include those known in the art, such as AGRIMER VA 6 and LICOWAX KST. Of note as adhesives are polyvinylpyrrolidinone-vinyl acetate copolymers and water-soluble waxes (e.g., polyethylene glycol).
The total amount of adhesive (i.e., the sum of component (b) and adhesives in component (d)) in the composition adhering to a coated propagule is generally in the range of about 0.001 to 100% of the weight of the propagule. For large seeds, the total amount of adhesive is typically in the range of about 0.05 to 5% of the seed weight; for small seeds the total amount is typically in the range of about 1 to 100%, but can be greater than 100% of seed weight in pelleting. For other propagules, the total amount of adhesive is typically in the range of 0.001 to 2% of the propagule weight.
Optionally, the present composition can contain up to about 10% (based on the weight of the composition) of liquid diluents as a constituent of component (d). In the context of the present disclosure and claims, the term “liquid diluent” excludes water unless otherwise indicated. When the present composition comprises one or more liquid diluents, they generally amount to at least 0.1% of the composition by weight. Typically as a constituent in a composition coating a propagule, the liquid diluents are relatively nonvolatile, i.e., have a normal boiling point of greater than about 160° C., preferably greater than about 200° C. Examples of liquid diluents include N-alkylpyrrolidones, dimethyl sulfoxide, ethylene glycol, polypropylene glycol, propylene carbonate, dibasic esters, paraffins, alkylnaphthalenes, oils of olive, castor, linseed, tung, sesame, corn, peanut, cottonseed, soybean, rapeseed and coconut, fatty acid esters, ketones such as isophorone and 4-hydroxy-4-methyl-2-pentanone, and alcohols such as cyclohexanol, decanol, benzyl and tetrahydrofurfuryl alcohol. Typical liquid diluents are described in Marsden, Solvents Guide, 2nd Ed., Interscience, New York, 1950. As the presence of liquid diluents can soften a composition coating a propagule, the present composition typically comprises not more than about 5% of liquid diluents by weight.
Optionally, the present composition can contain up to about 75% (based on the weight of the composition) of solid diluents as a constituent of component (d). When the present composition comprises one or more solid diluents, they generally amount to at least 0.1% of the composition by weight. In the context of the present disclosure and claims, solid diluents are considered to be solid substances principally providing bulk instead of other useful (e.g., adhesive, surfactant) properties. Typical solid diluents are described in Watkins et al., Handbook of Insecticide Dust Diluents and Carriers, 2nd Ed., Dorland Books, Caldwell, N.J. Solid diluents include, for example, clays such as bentonite, montmorillonite, attapulgite and kaolin, starch, sugar, silica, talc, diatomaceous earth, urea, calcium carbonate, sodium carbonate and bicarbonate, and sodium sulfate. High concentrations of solid diluents (i.e., up to about 75%) are typically included in a composition of the present invention for pelleting seeds. For pelleting seeds, the solid diluents are preferably insoluble, for example, bentonite, montmorillonite, attapulgite and kaolin (clays), silica (e.g., powdered silica) and calcium carbonate (e.g., ground limestone). When the present composition is not intended for pelleting seeds, the amount of solid diluents is typically not more than about 10% of the composition by weight.
The branched copolymers of component (b) typically obviate the need to include additional surfactants such as wetting agents and dispersants, but one or more such surfactants can be included in the composition as a constituent of component (d). If the present composition includes additional wetting agents or dispersants, they typically are present in an amount of at least about 0.1% of the composition by weight. Typically the present composition does not include more than about 15%, more typically not more than about 10%, and most typically not more than about 5% of additional surfactants by weight.
Examples of dispersing agents include anionic surfactants such as phosphate esters of tristyrylphenol ethoxylates (e.g., SOPROPHOR 3D33), alkylarylsulfonic acids and their salts (e.g., SUPRAGIL MNS90), lignin sulfonates (e.g., ammonium lignosulfonate or sodium lignosulfonate), polyphenol sulfonates, polyacrylic acids, acrylic graft copolymers such as acrylic acid/methyl methacrylate/polyoxyethylene graft copolymers (e.g., ATLOX 4913), and other polymers combining polyoxyalkylene with acid functionality such as ATLOX 4912 (a block copolymer of polyoxyethylene and hydroxystearic acid).
Examples of wetting agents (some of which overlap with dispersing agents) include alkyl sulfate salts (e.g., SIPON LC 98, sodium lauryl sulfate), alkyl ether sulfate salts (e.g., sodium lauryl ether sulfate), alkylarylsulfonates (i.e., salts of alkylarylsulfonic acids, including arylsulfonic acids substituted with more than one alkyl moiety) such as sodium or calcium alkylbenzenesulfonates (e.g., RHODACAL DS1) and alkylnaphthalenesulfonates (e.g., RHODACAL BX-78), α-olefin sulfonate salts, dialkyl sulfosuccinate salts and salts of polycarboxylic acids.
Additional surfactants include, for example, ethoxylated alcohols, ethoxylated alkylphenols, ethoxylated sorbitan fatty acid esters, ethoxylated sorbitol fatty acid esters, ethoxylated amines, ethoxylated fatty acids and esters (including ethoxylated vegetable oils), organosilicones, N,N-dialkyltaurates, glycol esters, formaldehyde condensates, and block polymers other than the branched copolymers of component (b).
Component (d) can also comprise one or more anti-foaming agents. Anti-foaming agents are surfactants that can effectively either prevent foam formation or reduce or eliminate it once it has formed. Examples of anti-foaming agents include silicone oils, mineral oils, polydialkylsiloxanes such as polydimethylsiloxanes, fatty acids and their salts with polyvalent cations such as calcium, magnesium and aluminum, alkyne diols (e.g., SURFYNOL 104), and fluoroaliphatic esters, perfluoroalkylphosphonic and perfluoroalkylphosphinic acids, and salts thereof. When the present composition comprises one or more anti-foaming agents, they typically amount to at least about 0.01% and not more than about 3% of the composition by weight. More typically, anti-foaming agents are not more than about 2% and most typically not more than about 1% of the composition by weight.
McCutcheon's Emulsifiers and Detergents and McCutcheon's Functional Materials (North America and International Editions, 2001), The Manufacturing Confection Publ. Co., Glen Rock, N.J., as well as Sisely and Wood, Encyclopedia of Surface Active Agents, Chemical Publ. Co., Inc., New York, 1964, list surfactants and recommended uses.
Component (d) can comprise one or more antifreeze agents. Antifreeze agents prevent freezing of the composition of the present invention extended with an aqueous liquid carrier before coating on propagules. Examples of antifreeze agents include glycols such as ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, glycerol, 1,3-propanediol, 1,2-propanediol and polyethylene glycol of molecular weight in the range from about 200 to about 1000 daltons. Antifreeze agents of note for the composition of the present invention include ethylene glycol, propylene glycol, glycerol, 1,3-propanediol and 1,2-propanediol. When component (d) comprises one or more antifreeze agents, they typically amount to at least about 0.1% and not more than about 14% of the composition by weight. More typically, antifreeze agents do not amount to more than 10% and most typically not more than about 8% of the total weight of the composition.
Component (d) can comprise one or more thickening agents. Thickening agents (i.e., thickeners) increase the viscosity of the continuous liquid medium formed when the present composition is extended with an aqueous liquid carrier. By increasing viscosity, the propensity of solid particles (e.g., of component (a)) to settle is reduced. Because component (b) also increases viscosity, including one or more thickening agents in component (d) is generally not necessary and indeed can be unhelpful if the viscosity of the composition is already as much as desired. Including one or more thickening agents in component (d) can be beneficial for slowing settling of particles of component (a) if the composition is extended with a large amount of aqueous liquid carrier relative to component (b), particularly when component (b) comprises mainly branched copolymers of relatively low molecular weight (i.e., less than about 5,000 daltons). Examples of thickening agents useful for the present composition include polyols such as glycerol, polysaccharides including heteropolysaccharides such as xanthan gum, and hydrated clays with very small particle sizes (e.g., 2 nm) such as the hydrated magnesium aluminosilicate ACTI-GEL 208 (Active Minerals). Glycerol is of note as having both antifreeze and thickener properties. An extensive list of thickeners and their applications can be found in McCutcheon's 2005, Volume 2: Functional Materials published by MC Publishing Company. If component (d) comprises one or more thickening agents, they typically amount to at least about 0.1% and not greater than about 5% of the composition by weight.
Component (d) can comprise a preservative constituent consisting essentially of one or more stabilizing agents or biocides, and the amount of the preservative constituent is typically up to about 1% of the composition by weight. When a preservative constituent is present, it typically amounts to at least about 0.01% of the composition by weight. The preservative constituent does not exceed typically about 1%, more typically about 0.5% and most typically about 0.3% of the total weight of the composition.
Stabilizing agents, for example, anti-oxidants (such as butylhydroxytoluene) or pH modifiers (such as citric acid or acetic acid) can prevent decomposition of active ingredients (i.e., component (a) and/or component (c)) during storage. Biocides can prevent or reduce microbial contamination within a formulated composition. Particularly suitable biocides are bactericides such as LEGEND MK (a mixture of 5-chloro-2-methyl-3(2H)-isothiazolone with 2-methyl-3(2H)-isothiazolone), EDTA (ethylenediaminetetraacetic acid), formaldehyde, benzoic acid, and 1,2-benzisothiazol-3(2H)-one or its salts (e.g., PROXEL BD or PROXEL GXL (Arch)). Of note is the present composition wherein component (d) comprises a biocide, in particular, a bactericide such as 1,2-benzisothiazol-3(2H)-one or one of its salts.
Component (d) can also comprise one or more fertilizers. Fertilizers included in component (d) can provide plant nutrients such as nitrogen, phosphorus and potassium and/or micronutrients such as manganese, iron, zinc and molybdenum. Of note for inclusion in component (d) are micronutrients such as manganese, iron, zinc and molybdenum. If one or more fertilizers are present, they typically amount to at least about 0.1% and not more than about 20% of the composition by weight, although greater amounts can be included.
Other formulation ingredients can be included in the present composition as component (d), such as rheology modifiers, dyes, and the like. These ingredients are known to one skilled in the art and can be found described, for example, in McCutcheon's, Volume 2: Functional Materials published by MC Publishing Company annually.
One aspect of the present invention is a geotropic propagule coated with an insecticidally effective amount of the aforedescribed composition. Geotropic propagules include seeds. The present invention is applicable to virtually all seeds, including seeds of wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), barley (Hordeum vulgare L.), oat (Avena sativa L.), rye (Secale cereale L.), maize (Zea mays L.), sorghum (Sorghum vulgare Pers.), rice (Oryza sativa L.), wild rice (Zizania aquatica L.), cotton (Gossypium barbadense L. and G. hirsutum L.), flax (Linum usitatissimum L.), sunflower (Helianthus annuus L.), soybean (Glycine max Merr.), garden bean (Phaseolus vulgaris L.), lima bean (Phaseolus limensis Macf.), broad bean (Vicia faba L.), garden pea (Pisum sativum L.), peanut (Arachis hypogaea L.), alfalfa (Medicago sativa L.), beet (Beta vulgaris L.), garden lettuce (Lactuca sativa L.), rapeseed (Brassica rapa L. and B. napus L.), cole crops such as cabbage, cauliflower and broccoli (Brassica oleracea L.), turnip (Brassica rapa L.), leaf (oriental) mustard (Brassica juncea Coss.), black mustard (Brassica nigra Koch), tomato (Lycopersicon esculentum Mill.), potato (Solanum tuberosum L.), pepper (Capsicum frutescens L.), eggplant (Solanum melongena L.), tobacco (Nicotiana tabacum), cucumber (Cucumis sativus L.), muskmelon (Cucumis melo L.), watermelon (Citrullus vulgaris Schrad.), squash (Curcurbita pepo L., C. moschata Duchesne. and C. maxima Duchesne.), carrot (Daucus carota L.), zinnia (Zinnia elegans Jacq.), cosmos (e.g., Cosmos bipinnatus Cay.), chrysanthemum (Chrysanthemum spp.), sweet scabious (Scabiosa atropurpurea L.), snapdragon (Antirrhinum majus L.), gerbera (Gerbera jamesonii Bolus), babys-breath (Gypsophila paniculata L., G. repens L. and G. elegans Bieb.), statice (e.g., Limonium sinuatum Mill., L. sinense Kuntze.), blazing branched (e.g., Liatris spicata Willd., L. pycnostachya Michx., L. scariosa Willd.), lisianthus (e.g., Eustoma grandiflorum (Raf.) Shinn), yarrow (e.g., Achillea filipendulina Lam., A. millefolium L.), marigold (e.g., Tagetes patula L., T. erecta L.), pansy (e.g., Viola cornuta L., V. tricolor L.), impatiens (e.g., Impatiens balsamina L.), petunia (Petunia spp.), geranium (Geranium spp.) and coleus (e.g., Solenostemon scutellarioides (L.) Codd). Geotropic propagules also include rhizomes, tubers, bulbs or corms, or viable divisions thereof. Suitable rhizomes, tubers, bulbs and corms, or viable divisions thereof include those of potato (Solanum tuberosum L.), sweet potato (Ipomoea batatas L.), yam (Dioscorea cayenensis Lam. and D. rotundata Poir.), garden onion (e.g., Allium cepa L.), tulip (Tulipa spp.), gladiolus (Gladiolus spp.), lily (Lilium spp.), narcissus (Narcissus spp.), dahlia (e.g., Dahlia pinnata Cay.), iris (Iris germanica L. and other species), crocus (Crocus spp.), anemone (Anemone spp.), hyacinth (Hyacinth spp.), grape-hyacinth (Muscari spp.), freesia (e.g., Freesia refracta Klatt., F. armstrongii W. Wats), ornamental onion (Allium spp.), wood-sorrel (Oxalis spp.), squill (Scilla peruviana L. and other species), cyclamen (Cyclamen persicum Mill. and other species), glory-of-the-snow (Chionodoxa luciliae Boiss. and other species), striped squill (Puschkinia scilloides Adams), calla lily (Zantedeschia aethiopica Spreng., Z. elliottiana Engler and other species), gloxinia (Sinnigia speciosa Benth. & Hook.) and tuberous begonia (Begonia tuberhybrida Voss.). The above recited cereal, vegetable, ornamental (including flower) and fruit crops are illustrative, and should not be considered limiting in any way. For reasons of insect control spectrum and economic importance, embodiments coating seeds of cotton, maize, soybean, rapeseed and rice, and coating tubers and bulbs of potato, sweet potato, garden onion, tulip, daffodil, crocus and hyacinth are of note. Also of note are embodiments wherein the geotropic propagule is a seed.
The present composition can be coated on geotropic propagules that contain genetic material introduced by genetic engineering (i.e., transgenic) or modified by mutagenesis to provide advantageous traits. Examples of such traits include tolerance to herbicides, resistance to phytophagous pests (e.g., insects, mites, aphids, spiders, nematodes, snails, plant-pathogenic fungi, bacteria and viruses), improved plant growth, increased tolerance of adverse growing conditions such as high or low temperatures, low or high soil moisture, and high salinity, increased flowering or fruiting, greater harvest yields, more rapid maturation, higher quality and/or nutritional value of the harvested product, or improved storage or process properties of the harvested products. Transgenic plants can be modified to express multiple traits. Examples of plants containing traits provided by genetic engineering or mutagenesis include varieties of corn, cotton, soybean and potato expressing an insecticidal Bacillus thuringiensis toxin such as YIELD GARD, KNOCKOUT, STARLINK, BOLLGARD, NuCOTN and NEWLEAF, and herbicide-tolerant varieties of corn, cotton, soybean and rapeseed such as ROUNDUP READY, LIBERTY LINK, IMI, STS and CLEARFIELD, as well as crops expressing N-acetyltransferase (GAT) to provide resistance to glyphosate herbicide, or crops containing the HRA gene providing resistance to herbicides inhibiting acetolactate synthase (ALS). The present insecticidal composition may interact synergistically with traits introduced by genetic engineering or modified by mutagenesis, thus enhancing phenotypic expression or effectiveness of the traits or increasing the insect control effectiveness of the present composition. In particular, the present insecticidal composition may interact synergistically with the phenotypic expression of proteins or other natural products toxic to invertebrate pests to provide greater-than-additive control of these pests.
The thickness of coatings of the present composition on geotropic propagules can vary from thin films 0.001 mm thick to layers about 0.5 to 5 mm thick. Generally, a coating that increases the weight of a seed up to 25% is defined as a film coating. Film-coated seed retains the shape and the general size of the uncoated seed. A coating that increases the weight of the seed more than 25% is referred to as a pellet coating. Coatings on geotropic propagules can comprise more than one adhering layer, only one of which need comprise the present composition. Generally pellets are more satisfactory for small seeds, because their ability to provide an insecticidally effective amount of the present composition is not limited by the surface area of the seed, and pelleting small seeds also facilitates seed transfer and planting operations. Because of their larger size and surface area, large seeds and bulbs, tubers, corms and rhizomes and their viable cuttings are generally not pelleted, but instead coated with a thin film.
For application of a coating of the aforedescribed composition to a geotropic propagule, the composition is typically first extended with a volatile aqueous liquid carrier to provide a liquid composition consisting of about 5 to 80 weight % of the aforedescribed (unextended) composition (i.e., mixture comprising components (a), (b) and optionally (c) and (d)) and about 20 to 95 weight % of the volatile aqueous liquid carrier. Alternatively and more typically, one or more of the composition components is first mixed with the volatile aqueous liquid carrier before the components are combined to provide the liquid composition containing components (a), (b) and optionally (c) and (d) in combination with about 20-95 weight % of the volatile aqueous liquid carrier. The amount of volatile liquid carrier is more typically at least about 25% and most typically at least about 30% of the liquid composition by weight. Also, the amount of volatile liquid carrier is more typically not more than about 70% of the liquid composition by weight.
In the context of the present disclosure and claims, the expression “volatile aqueous liquid carrier” refers to a composition consisting of at least about 50% water by weight and optionally one or more water-soluble compounds that are liquid at 20° C. and have a normal boiling point of not greater than about 100° C. These water-soluble liquid compounds should be nonphytotoxic to the geotropic propagule to be coated. Examples of such water-soluble liquid compounds are acetone, methyl acetate, methanol and ethanol. However, a volatile aqueous liquid carrier mostly or entirely of water is typically preferable, because water is inexpensive, nonflammable, environmentally friendly and nonphytotoxic. Typically the volatile aqueous liquid carrier comprises at least about 80%, more typically at least about 90%, and most typically at least about 95% water by weight. In some embodiments, the volatile aqueous liquid carrier consists essentially of water. In some embodiments, the volatile liquid carrier is water.
In the liquid composition comprising the volatile aqueous liquid carrier, the volatile aqueous liquid carrier forms a continuous liquid phase in which other components (e.g., components (a), (b) and optionally (c) and (d)) are suspended or dissolved. Typically at least some of component (a) is present as particles suspended in the continuous liquid phase and therefore the liquid composition can be described as a suspension concentrate composition. In some embodiments at least about 90%, or 95% or 98% of component (a) is present as particles suspended in the continuous liquid phase. Typically more than 95% by weight of the particles have a particle size less than about 10 microns.
The aggregation state of the branched copolymers component (i.e., component (b)) in the liquid composition depends on such parameters as ingredients, concentration, temperature and ionic strength. The liquid composition typically comprises suspended particles of component (a) having large surface areas. Acrylate/methacrylate-based branched copolymer molecules are generally adsorbed to such interfaces (e.g., as monolayers, bilayers or hemimicelles) in preference to remaining in solution, and only when the interfaces are saturated do high concentrations of the molecules remain in the aqueous phase. Therefore the presence of particles of component (a) allows the liquid composition to accommodate more of component (b) without forming a separate component (b) phase than would be expected based solely on water solubility or dispersibility. If the liquid composition contains component (b) in excess of both its adsorption onto component (a) particles and its solubility in the aqueous carrier phase, a portion of component (b) will be present in a discrete phase, either as solid particles or as liquid droplets depending upon the physical properties (e.g., melting point) of component (b).
The liquid composition comprising the volatile aqueous liquid carrier is often most conveniently prepared by mixing components (a) and (b) and optionally (c) and (d) with the volatile aqueous liquid carrier (e.g., water). As noted above, component (b) is water soluble or dispersible to the extent of at least 5% at 20° C. For ease of dissolution of component (b) in the formulation, it is preferred to dissolve component (b) in the aqueous liquid carrier prior to mixing with the other ingredients.
In the liquid composition, the median particle size of particles of component (a) is preferably less than about 10 microns to provide good suspensibility as well as high biological availability and coating coverage of the propagule. More preferably the median particle size of component (a) is less than 4 microns or 3 microns or 2 microns and most preferably less than about 1 micron. Typically the median particle size is at least about 0.1 micron, but smaller particle sizes are suitable.
Milling can be used to reduce the particle size of component (a) as well as other solid components. Milling methods are well-known and include ball-milling, bead-milling, sand-milling, colloid milling and air-milling. These can be combined with high-speed blending, which typically involves high shear, to prepare suspensions and dispersions of particles. Of particular note is ball- or bead-milling for reducing the particle size of component (a). Other components, such as component (b), can be included in the mixture for milling or later mixed with the milled mixture. However, other components comprising solid particles initially having a particle size of greater than 10 microns and low water solubility are typically included in the mixture for milling. Although branched copolymer component (b) and optional additional surfactant of component (d) can be added after milling component (a), typically a portion of component (b) and/or optional additional surfactant is included in the mixture to facilitate milling component (a) to small particle size.
Milling is often unneeded in methods for preparing the liquid composition by first dissolving component (a) in an organic solvent. In one method, components (a) and (b) and optionally other components are dissolved in an organic solvent, and then a miscible solvent in which components (a) and (b) are much less soluble is added to the solution of components (a) and (b) to form a precipitate. The precipitate is collected and suspended in the volatile aqueous liquid carrier (e.g., water) for coating propagules.
In a related method, components (a) and (b) and optionally other components are dissolved in an organic solvent system comprising a lower boiling solvent in which component (a) is very soluble and a higher boiling solvent in which component (a) is less soluble (e.g., a binary solvent system of dichloromethane and ethanol), and then the solvent is evaporated under vacuum. The residue is then suspended in the volatile aqueous liquid carrier (e.g., water) for coating propagules.
In another method, component (a) and component (b) are dissolved in a water-miscible organic solvent such as N-methyl-2-pyrrolidone. The solution is then placed inside a sealed dialysis membrane, which is selected to allow the organic solvent and water to equilibrate but not allow passage of component (b). The sealed dialysis membrane is then placed in water to allow replacement of the organic solvent with water. Water entering the dialysis membrane causes component (a) to crystallize and form a slurry. The resultant aqueous slurry is used to coat propagules.
After the liquid composition comprising the volatile aqueous liquid carrier has been prepared, it can be applied to the surface of a propagule by any of several techniques known in the art, which involve evaporating the volatile aqueous liquid carrier to leave a coating of the insecticidal composition comprising components (a), (b) and optionally (c) and (d) adhering to the surface of the propagule. Various coating machines and processes are available to one skilled in the art. Suitable processes include those listed in P. Kosters et al., Seed Treatment: Progress and Prospects, 1994 BCPC Monograph No. 57 and the references listed therein. Coating processes are also described in U.S. Pat. Nos. 5,527,760 and 6,202,345. Three well-known techniques include the use of drum coaters, fluidized bed techniques and spouted beds. Seeds can be presized prior to coating. After coating, the seeds are dried and then optionally sized by transfer to a sizing machine. These machines are known in the art.
In one method, propagules are coated by spraying the liquid composition comprising the volatile aqueous liquid carrier directly into a tumbling bed of seeds and then drying the propagules. In one embodiment for coating seeds, the seed and coating material are mixed in a conventional seed coating apparatus. The rate of rolling and application of coating depends upon the seed. For large oblong seeds such as that of cotton, a satisfactory seed coating apparatus comprises a rotating type pan with lifting vanes turned at sufficient rpm to maintain a rolling action of the seed, facilitating uniform coverage. The seed coating must be applied over sufficient time to allow drying to minimize clumping of the seed. Using forced air or heated forced air can allow increasing the rate of application. One skilled in the art will also recognize that this process may be a batch or continuous process. As the name implies, a continuous process allows the seeds to flow continuously throughout the product run. New seeds enter the pan in a steady stream to replace coated seeds exiting the pan.
One embodiment of seed coating is seed pelleting. The pelleting process typically increases the seed weight from 2 to 100 times and can be used to also improve the shape of the seed for use in mechanical seeders. Pelleting compositions generally contain a solid diluent, which is typically an insoluble particulate material, such as clay, ground limestone, or powdered silica to provide bulk in addition to a film-former or sticking agent. Depending on the extent of coating applied, pelletizing may provide a spherical shape to the seeds which are normally elongated or irregularly shaped. A method for producing pellets is described in Agrow, The Seed Treatment Market, Chapter 3, PJB Publications Ltd., 1994.
One aspect of the present invention is a method for protecting a geotropic propagule and plant derived therefrom from a phytophagous insect pest by coating the propagule with an insecticidally effective amount of the liquid composition comprising components (a), (b) and optionally (c) and (d) along with a volatile aqueous liquid carrier and then evaporating the volatile aqueous liquid carrier of the composition. This coating process constitutes a treatment of the propagule by providing a coating of an insecticidally effective amount of the insecticidal composition on the propagule. The coating of the composition on the propagule provides an insecticidally effective amount of component (a) (i.e., one or more anthranilic diamide insecticides) available for absorption into the propagule and/or roots developing from the propagule. In some embodiments, the branched copolymer of component (b) have been discovered to increase the absorption of component (a) into the propagules and/or developing roots to provide through xylem transport an insecticidally effective concentration of component (a) in even foliage developing from the coated propagule. Sufficiently increasing the absorption can raise concentrations of component (a) above the minimum concentration for insecticidal effectiveness in not only the lower foliage but also middle to upper foliage, and provide protection later into the growing season. Insecticidally effective concentrations of component (a) protect the propagule and derived plant from injury or damage caused by a phytophagous insect pest by controlling the insect pest. This control can include killing the insect pest, interfering with its growth, development or reproduction, and/or inhibiting its feeding. Typically, control involves feeding inhibition and death of the insect pest.
Generally to protect a seed and foliage developing therefrom from a phytophagous insect pest, the present composition is coated on a geotropic propagule to provide component (a) in an amount ranging from about 0.001 to 50% of the weight of the propagule, for seeds more often in the range of about 0.01 to 50% of the seed weight, and most typically for large seeds in the range of about 0.01 to 10% of the seed weight. However, larger amounts up to about 100% or more are useful, particularly for pelleting small seed for extended invertebrate pest control protection. For propagules such as bulbs, tubers, corms and rhizomes and their viable cuttings, generally the amount of component (a) included in the composition coating ranges from about 0.001 to 5% of the propagule weight, with the higher percentages used for smaller propagules. One skilled in the art can easily determine the insecticidally effective amount of the present composition and component (a) necessary for the desired level of phytophagous insect pest control and seed and plant protection.
As referred to in this disclosure, the term “phytophagous insect pest” includes larvae of the order Lepidoptera, such as armyworms, cutworms, loopers, and heliothines in the family Noctuidae (e.g., fall armyworm (Spodoptera fugiperda J. E. Smith), beet armyworm (Spodoptera exigua Hübner), black cutworm (Agrotis ipsilon Hufnagel), cabbage looper (Trichoplusia ni Hübner), and tobacco budworm (Heliothis virescens Fabricius)); borers, casebearers, webworms, coneworms, cabbageworms and skeletonizers from the family Pyralidae (e.g., European corn borer (Ostrinia nubilalis Hübner), navel orangeworm (Amyelois transitella Walker), corn root webworm (Crambus caliginosellus Clemens), and sod webworm (Herpetogramma licarsisalis Walker)); leafrollers, budworms, seed worms, and fruit worms in the family Tortricidae (e.g., codling moth (Cydia pomonella L. (L. means Linnaeus)), grape berry moth (Endopiza viteana Clemens), and oriental fruit moth (Grapholita molesta Busck)); and many other economically important lepidoptera (e.g., diamondback moth (Plutella xylostella L. of family Plutellidae), pink bollworm (Pectinophora gossypiella Saunders of family Gelechiidae), and gypsy moth (Lymantria dispar L. of family Lymantriidae)); foliar feeding larvae and adults of the order Coleoptera including weevils from the families Anthribidae, Bruchidae, and Curculionidae (e.g., boll weevil (Anthonomus grandis Boheman), rice water weevil (Lissorhoptrus oryzophilus Kuschel), and rice weevil (Sitophilus oryzae L.)); flea beetles, cucumber beetles, rootworms, leaf beetles, potato beetles, and leafminers in the family Chrysomelidae (e.g., Colorado potato beetle (Leptinotarsa decemlineata Say), and western corn rootworm (Diabrotica virgifera virgifera LeConte)); chafers and other beetles from the family Scaribaeidae (e.g., Japanese beetle (Popillia japonica Newman) and European chafer (Rhizotrogus majalis Razoumowsky)); wireworms from the family Elateridae and bark beetles from the family Scolytidae; adults and larvae of the order Dermaptera including earwigs from the family Forficulidae (e.g., European earwig (Forficula auricularia L.) and black earwig (Chelisoches morio Fabricius)); adults and nymphs of the orders Hemiptera and Homoptera such as, plant bugs from the family Miridae, cicadas from the family Cicadidae, leafhoppers (e.g., Empoasca spp.) from the family Cicadellidae, planthoppers from the families Fulgoroidae and Delphacidae, treehoppers from the family Membracidae, psyllids from the family Psyllidae, whiteflies from the family Aleyrodidae, aphids from the family Aphididae, phylloxera from the family Phylloxeridae, mealybugs from the family Pseudococcidae, scales from the families Coccidae, Diaspididae and Margarodidae, lace bugs from the family Tingidae, stink bugs from the family Pentatomidae, cinch bugs (e.g., Blissus spp.) and other seed bugs from the family Lygaeidae, spittlebugs from the family Cercopidae, squash bugs from the family Coreidae, and red bugs and cotton stainers from the family Pyrrhocoridae; adults and immatures of the order Orthoptera including grasshoppers, locusts and crickets (e.g., migratory grasshoppers (e.g., Melanoplus sanguinipes Fabricius and M. differentialis Thomas), American grasshoppers (e.g., Schistocerca americana Drury), desert locust (Schistocerca gregaria Forskal), migratory locust (Locusta migratoria L.), and mole crickets (Gryllotalpa spp.)); adults and immatures of the order Diptera, including leafminers, midges, fruit flies (Tephritidae), frit flies (e.g., Oscinella frit L.), soil maggots and other Nematocera; adults and immatures of the order Thysanoptera including onion thrips (Thrips tabaci Lindeman) and other foliar feeding thrips. Of note is the present method for protecting a propagule or plant derived therefrom from a phytophagous insect pest wherein the insect pest is in a taxonomic order selected from Hemiptera (particularly the families Aleyrodidae, Aphidadae, Cicadellidae, and Delphacidae) and Lepidoptera (particularly the families Gelechiidae, Lymantriidae, Noctuidae, Plutellidae, Pyralidae and Torticidae). Of particular note is the present method wherein the insect pest is in the family Noctuidae.
Embodiments of the present invention include:
Embodiment 1
The insecticidal composition described in the summary of the invention comprising by weight based on the total weight of the composition:

    
    
        (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and
        (b) from about 9 to about 91% of a branched copolymer component having a water solubility or dispersibility of at least about 5% by weight at 20° C.,
        wherein:
        the branched copolymer component comprises one or more branch segments attached to a linear backbone segment;
        the branch segments comprise at least two repeating monomer units and have a number average molecular weight (Mn) of at least about 1,000;
        the branched copolymer component comprises hydrophilic groups; and
        the ratio of component (b) to component (a) is about 1:10 to about 10:1 by weight.
    
    


Embodiment 2
The composition of Embodiment 1 wherein component (a) (i.e., one or more anthranilic diamide insecticides) comprises at least one compound selected from anthranilic diamides of Formula 1, N-oxides, and salts thereof,




wherein

X is N, CF, CCl, CBr or Cl;
R1 is CH3, Cl, Br or F;
R2 is H, F, Cl, Br or —CN;
R3 is F, Cl, Br, C1-C4 haloalkyl or C1-C4 haloalkoxy;
R4a is H, C1-C4 alkyl, cyclopropylmethyl or 1-cyclopropylethyl;
R4b is H or CH3;
R5 is H, F, Cl or Br; and
R6 is H, F, Cl or Br.
Embodiment 3
The composition of Embodiment 2 wherein component (a) is selected from anthranilic diamides of Formula 1, N-oxides, and salts thereof.
Embodiment 4
The composition of Embodiment 3 wherein component (a) is selected from anthranilic diamides of Formula 1 and salts thereof.
Embodiment 5
The composition of Embodiment 4 wherein component (a) is selected from anthranilic diamides of Formula 1.
Embodiment 6
The composition of any one of Embodiments 2 through 5 wherein X is N; R1 is CH3; R2 is Cl or —CN; R3 is Cl, Br or CF3; R4a is C1-C4 alkyl; R4b is H; R5 is Cl; and R6 is H.
Embodiment 7
The composition of Embodiment 6 wherein R4a is CH3 or CH(CH3)2.
Embodiment 8
The composition of Embodiment 7 wherein R3 is Br; and R4a is CH3 (i.e., the compound of Formula 1 is chlorantraniliprole or cyantraniliprole, or optionally an N-oxide or salt thereof).
Embodiment 9
The composition of Embodiment 8 wherein R2 is Cl (i.e., the compound of Formula 1 is chlorantraniliprole, or optionally an N-oxide or salt thereof).
Embodiment 10
The composition of Embodiment 8 wherein R2 is —CN (i.e., the compound of Formula 1 is cyantraniliprole, or optionally an N-oxide or salt thereof).
Embodiment 11
The composition of any one of Embodiments 1 through 10 wherein component (a) is at least about 10% of the composition by weight.
Embodiment 12
The composition of Embodiment 11 wherein component (a) is at least about 20% of the composition by weight.
Embodiment 13
The composition of Embodiment 12 wherein component (a) is at least about 30% of the composition by weight.
Embodiment 14
The composition of Embodiment 13 wherein component (a) is at least about 40% of the composition by weight.
Embodiment 15
The composition of any one of Embodiments 1 through 14 wherein component (a) is not more than about 90% of the composition by weight.
Embodiment 16
The composition of Embodiment 15 wherein component (a) is not more than about 80% of the composition by weight.
Embodiment 17
The composition of Embodiment 16 wherein component (a) is not more than about 70% of the composition by weight.
Embodiment 18
The composition of any one of Embodiments 1 through 17 wherein not more than about 30% of component (a) is present in the composition as solid particles having a particle size greater than about 10 microns.
Embodiment 19
The composition of Embodiment 18 wherein not more than about 20% of component (a) is present in the composition as solid particles having a particle size greater than about 10 microns.
Embodiment 20
The composition of Embodiment 20 wherein not more than about 10% of component (a) is present in the composition as solid particles having a particle size greater than about 10 microns.
Embodiment 21
The composition of any one of Embodiments 1 through 20 wherein component (b) (i.e., the branched copolymer component) has a water solubility or dispersibility of at least about 10% at 20° C.
Embodiment 22
The composition of Embodiment 21 wherein component (b) has a water solubility or dispersibility of at least about 25% at 20° C.
Embodiment 23
The composition of any one of Embodiments 1 through 22 wherein component (b) (separate from the composition) is a paste or solid at 20° C.
Embodiment 24
The composition of any one of Embodiments 1 through 23 wherein component (b) (separate from the composition) is a solid at 20° C.
Embodiment 25
The composition of any one of Embodiments 1 through 24 wherein component (b) comprises one or more branch segments attached to a linear backbone segment.
Embodiment 26
The composition of Embodiment 25 wherein component (b) has a number average molecular weight (Mn) of about 2,000-500,000.
Embodiment 27
The composition of Embodiment 26 wherein component (b) has a number average molecular weight (Mn) of about 2,000-50,000.
Embodiment 28
The composition of Embodiment 27 wherein component (b) has a number average molecular weight (Mn) of about 2,000-30,000.
Embodiment 29
The composition of Embodiment 25 to 28 wherein component (b) comprises branch segments with a number average molecular weight (Mn) of about 1,000-15,000.
Embodiment 30
The composition of Embodiment 29 wherein component (b) comprises branch segments with a number average molecular weight (Mn) of about 1,000-8,000.
Embodiment 31
The composition of Embodiment 30 wherein component (b) comprises branch segments with a number average molecular weight (Mn) of about 1,000-5,000.
Embodiment 32
The composition of Embodiment 25 to 31 wherein the weight ratio of the linear backbone segment to the branch segments of component (b) is between about 50/1 to about 1/10.
Embodiment 33
The composition of Embodiment 32 wherein the weight ratio is between about 80/20 to about 60/40.
Embodiment 34
The composition of any one of Embodiments 1 through 33 wherein component (b) is at least about 10% of the composition by weight.
Embodiment 35
The composition of Embodiment 34 wherein component (b) is at least about 15% of the composition by weight.
Embodiment 36
The composition of Embodiment 35 wherein component (b) is at least about 20% of the composition by weight.
Embodiment 37
The composition of Embodiment 36 wherein component (b) is at least about 25% of the composition by weight.
Embodiment 38
The composition of Embodiment 37 wherein component (b) is at least about 30% of the composition by weight.
Embodiment 39
The composition of Embodiment 38 wherein component (b) is at least about 35% of the composition by weight.
Embodiment 40
The composition of Embodiment 39 wherein component (b) is at least about 40% of the composition by weight.
Embodiment 41
The composition of any one of Embodiments 1 through 40 wherein component (b) is not more than about 80% of the composition by weight.
Embodiment 42
The composition of Embodiment 41 wherein component (b) is not more than about 70% of the composition by weight.
Embodiment 43
The composition of Embodiment 42 wherein component (b) is not more than about 60% of the composition by weight.
Embodiment 43
The composition of Embodiment 42 wherein component (b) is not more than about 50% of the composition by weight.
Embodiment 44
The composition of Embodiment 43 wherein component (b) is not more than about 40% of the composition by weight.
Embodiment 45
The composition of any one of Embodiments 1 through 44 wherein the ratio of component (b) to component (a) is at least about 1:8 (by weight).
Embodiment 46
The composition of Embodiment 45 wherein the ratio of component (b) to component (a) is at least about 1:5.
Embodiment 47
The composition of Embodiment 46 wherein the ratio of component (b) to component (a) is at least about 1:4.
Embodiment 48
The composition of Embodiment 47 wherein the ratio of component (b) to component (a) is at least about 1:3.
Embodiment 49
The composition of Embodiment 48 wherein the ratio of component (b) to component (a) is at least about 1:2.
Embodiment 50
The composition of Embodiment 49 wherein the ratio of component (b) to component (a) is at least about 1:1.
Embodiment 51
The composition of Embodiment 50 wherein the ratio of component (b) to component (a) is at least about 2:1.
Embodiment 52
The composition of Embodiment 51 wherein the ratio of component (b) to component (a) is at least about 3:1.
Embodiment 53
The composition of Embodiment 52 wherein the ratio of component (b) to component (a) is at least about 4:1.
Embodiment 53
The composition of Embodiment 52 wherein the ratio of component (b) to component (a) is at least about 8:1.
Embodiment 54
The composition of any one of Embodiments 1 through 53 wherein the ratio of component (b) to component (a) is not more than about 1:1.
Embodiment 55
The composition described in the Summary of the Invention or any one of Embodiments 1 through 54 wherein component (b) comprises one or more branched copolymers.
Embodiment 56
The composition of Embodiment 55 wherein component (b) comprises one or more branched copolymers.
Embodiment 57
The composition of Embodiment 55 or 56 wherein component (b) comprises one or more branched copolymers.
Embodiment 58
The composition of Embodiment 57 wherein component (b) consists essentially of one or more branched copolymers.
Embodiment 59
The composition of any one of Embodiments 1 to 58 wherein component (c) comprises one or more biologically active agents other than anthranilic diamide insecticides and is at least 0.1% of the composition by weight.
Embodiment 60
The composition of Embodiment 59 wherein component (c) is at least 1% of the composition by weight.
Embodiment 61
The composition of Embodiment 59 or 60 wherein component (c) is not more than about 60% of the composition by weight.
Embodiment 62
The composition of Embodiment 61 wherein component (c) is not more than about 20% of the composition by weight.
Embodiment 63
The composition of any one of Embodiments 59 through 62 wherein component (c) comprises at least one fungicide or insecticide (other than anthranilic diamide insecticides).
Embodiment 64
The composition of Embodiment 63 wherein component (c) comprises at least one insecticide.
Embodiment 65
The composition of Embodiment 63 or 64 wherein component (c) comprises at least one fungicide.
Embodiment 66
The composition of any one of Embodiments 1 through 62 wherein the composition does not comprise a biologically active agent other than component (a).
Embodiment 67
The composition of any one of Embodiments 1 through 66 wherein the composition further comprises (d) up to about 80% by weight of one or more inert formulating ingredients other than branched copolymers.
Embodiment 68
The composition of Embodiment 67 wherein component (d) (i.e., the one or more inert formulating ingredients other than branched copolymers) is at least about 0.1% of the composition by weight.
Embodiment 69
The composition of Embodiment 67 or 68 wherein component (d) is not more than about 20% of the composition by weight.
Embodiment 70
The composition of any one of Embodiments 67 through 69 wherein component (d) comprises at least one inert formulating ingredient selected from the group consisting of adhesives, liquid diluents, solid diluents, surfactants, antifreeze agents, preservatives, thickening agents and fertilizers.
Embodiment 71
The geotropic propagule described in the Summary of the Invention which is coated with an insecticidally effective amount of the composition of any one of Embodiments 1 through 70.
Embodiment 72
The geotropic propagule of Embodiment 71 which is a seed.
Embodiment 73
The seed of Embodiment 72 which is a seed of cotton, maize, soybean, rapeseed or rice.
Embodiment 74
The seed of Embodiment 73 which is a seed of maize or rapeseed.
Embodiment 75
The seed of Embodiment 74 which is a seed of maize.
Embodiment 76
The seed of Embodiment 75 which is a seed of rapeseed.
Embodiment 77
The liquid composition described in the Summary of the Invention consisting of about 5 to 80 weight % of the composition of any one of Embodiments 1 through 70 and about 20 to 95 weight % of a volatile aqueous liquid carrier.
Embodiment 78
The liquid composition of Embodiment 77 wherein the volatile aqueous liquid carrier is at least about 25% of the composition by weight.
Embodiment 79
The liquid composition of Embodiment 78 wherein the volatile aqueous liquid carrier is at least about 30% of the composition by weight.
Embodiment 80
The liquid composition of any one of Embodiments 77 through 79 wherein the aqueous liquid carrier is not more than about 70% of the composition by weight.
Embodiment 81
The liquid composition of any one of Embodiments 77 through 79 wherein the volatile aqueous liquid carrier comprises at least about 80% water by weight.
Embodiment 82
The liquid composition of Embodiment 81 wherein the volatile aqueous liquid carrier comprises at least about 90% water by weight.
Embodiment 83
The liquid composition of Embodiment 82 wherein the volatile aqueous liquid carrier comprises at least about 95% water by weight.
Embodiment 84
The liquid composition of Embodiment 83 wherein the volatile aqueous liquid carrier consists essentially of water.
Embodiment 85
The liquid composition of Embodiment 84 wherein the volatile aqueous liquid carrier is water.
Embodiment 86
The liquid composition of any one of Embodiments 77 through 85 wherein at least some of component (a) is present in the liquid composition as solid particles.
Embodiment 87
The liquid composition of Embodiment 86 wherein at least about 90% of component (a) is present in the composition as solid particles.
Embodiment 88
The liquid composition of Embodiment 87 wherein at least about 95% of component (a) is present in the composition as solid particles.
Embodiment 89
The liquid composition of Embodiment 88 wherein at least about 98% of component (a) is present in the composition as solid particles.
Embodiment 90
The liquid composition of any one of Embodiments 86 through 89 wherein more than 95% by weight of the particles have a particle size less than about 10 microns.
Embodiment 91
The liquid composition of any one of Embodiments 86 through 90 wherein the median particle size of the particles is not more than about 10 microns.
Embodiment 92
The liquid composition of Embodiment 90 or 91 wherein the median particle size of the particles is not more than about 4 microns.
Embodiment 93
The liquid composition of Embodiment 92 wherein the median particle size of the particles is not more than about 3 microns.
Embodiment 94
The liquid composition of Embodiment 93 wherein the median particle size of the particles in not more than about 2 microns.
Embodiment 95
The liquid composition of Embodiment 94 wherein the median particle size of the particles is not more than about 1 micron.
Embodiment 96
The liquid composition of any one of Embodiments 86 through 95 wherein the median particle size of the particles is at least about 0.1 micron.
Embodiment 97
The method described in the Summary of the Invention for protecting a geotropic propagule and plant derived therefrom from a phytophagous insect pest, the method comprising coating the propagule with an insecticidally effective amount of the liquid composition of any one of Embodiments 77 through 96 and then evaporating the volatile aqueous liquid carrier.
Embodiment 98
The method of Embodiment 97 wherein the insect pest is in a taxonomic order selected from Hemiptera and Lepidoptera.
Embodiment 99
The method of Embodiment 98 wherein the insect pest is in a taxonomic family selected from Aleyrodidae, Aphidadae, Cicadellidae, Delphacidae, Gelechiidae, Lymantriidae, Noctuidae, Plutellidae, Pyralidae and Torticidae.
Embodiment 100
The method of Embodiment 99 wherein the insect pest is in the family Noctuidae.
Embodiments of this invention can be combined in any manner. An example of such combination is the insecticidal composition described in the Summary of the Invention comprising by weight (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and (b) from about 9 to about 91% of a branched copolymer component having a water solubility or dispersibility of at least about 5% by weight at 20° C., wherein:

    
    
        the branched copolymer component comprises one or more branch segments attached to a linear backbone segment;
        the branch segments comprise at least two repeating monomer units and have a number average molecular weight (Mn) of at least about 1,000;
        the number average molecular weight (Mn) of the branched copolymer is between 2,000 and 12,000;
        the branched copolymer component comprises hydrophilic groups; and
        the ratio of component (b) to component (a) is about 1:5 to about 5:1 by weight.
    
    


Without further elaboration, it is believed that one skilled in the art using the preceding description can utilize the present invention to its fullest extent. The following Examples are, therefore, to be construed as merely illustrative and not limiting of the disclosure in any way whatsoever.
EXAMPLES
PCT Patent Publication WO 2006/062978 discloses methods for preparing 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide (i.e., Compound 1). Example 15 of this publication discloses preparation of Compound 1 as a powder melting at 177-181° C. (with apparent decomposition), which is a crystal form that is readily hydrated. Example 15 also discloses recrystallization from 1-propanol to provide crystals melting at 217-219° C., which is an anhydrous crystal form that is resistant to hydration. The samples of Compound 1 used in the present Examples and Comparative Examples were assayed to contain about 94-98% by weight of Compound 1, which is believed to be a mixture of these two crystal forms.
PCT Patent Publication WO 03/015519 discloses methods for preparing 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide (i.e., Compound 2). Example 7 of this publication discloses preparation of Compound 2 as a powder melting at 239-240° C. The samples of Compound 2 used in the present Examples and Comparative Examples were assayed to contain about 96-97% by weight of Compound 2.
The weight percentages of Compound 1 or 2 reported in the present Examples refer to the amount of Compound 1 or 2 contained in the technical material used; the other constituents in the technical material are not separately listed, but when added to weight percentages of the listed composition components result in a total of about 100%.
General Procedure for Coating Seeds
A fluidized bed system was used for coating seeds with the compositions described in the following examples. Seeds were tossed by vertical streams of hot air while being sprayed with the aqueous composition. The hot air evaporated the water carrier from the composition applied to the seeds. The amount of composition introduced into the coating system was adjusted to compensate for material lost exiting the coater or coating areas other than the seeds, so as to deliver the stated target application rate to the seeds.
General Procedure for Assaying Anthranilic Diamide Concentration in Leaves
Plant leaves were macerated using a Geno/Grinder 2000 bead beater homogenizer (SPEX CertiPrep, Metuchen, N.J., USA), and then acetonitrile ( ̃5 mL/g of leaf tissue) was added. The mixture was further shaken for 1 minute using the Geno/Grinder homogenizer, and then centrifuged. The acetonitrile extract supernatant was analyzed by high performance liquid chromatography with tandem mass spectrometry detection (HPLC/MS/MS) using a Waters (Milford, Mass. USA) Alliance HT2795 chromatograph and Zorbax SB C18 (2.1×50 mm, 5 micron) column eluted with mixtures of water and acetonitrile containing 0.1% (volume/volume) of formic acid, with detection by a Waters Quattro Micro API Mass Spectrometer using electrospray ionization (ESI+). Standard solutions of Compound 1 and Compound 2 were prepared by adding measured amounts of stock solutions of Compound 1 or Compound 2 in acetonitrile or tetrahydrofuran to acetonitrile extracts of leaves from plants grown from untreated seeds.
Examples 1-3
Synthesis and Purification
As used herein:
AA=Acrylic acid MAA=Methacrylic acid
BA=Butyl acrylate MMA=Methyl methacrylate
HEMA=Hydroxylethyl methacrylate PPM5=Polypropyleneglycol (5) methacrylate
HPMA=Hydroxypropyl methacrylate S=Styrene
MA=Methyl acrylate EHMA=2-Ethylhexyl methacrylate
Example 1
Synthesis of Polymer Dispersant 1
Polymer Dispersant 1 was produced according to the macromonomer polymerization process described in PCT Patent Publication WO1995/032228. The process is described briefly as follows. The macromonomer MMA/HEMA/MAA with monomer mixing weight ratio=26/14/10 was prepared according to the general process of Example 2 of the aforementioned PCT publication. The macromonomer was then polymerized in the presence of backbone monomers mixture S/PPM5/HEMA according to the general process of Example 10 described in the aforementioned PCT patent publication with the following monomer mixing weight ratio: S/PPM5/HEMA//MMA/HEMA/MAA=20/48/7//13/7/5%. The waterborne dispersant had % solids of 36.21 and pH of 7.96.
Example 2
Synthesis of Polymer Dispersant 2
Polymer Dispersant 2 was produced according to the macromonomer polymerization process described in PCT Patent Publication WO1995/032228. The process is described briefly as following. The macromonomer MMA/HEMA/MAA with monomer mixing weight ratio=26/14/10 was prepared according to the general process of Example 2 of the aforementioned PCT publication. The macromonomer was then polymerized in the presence of backbone monomers mixture S/EHMA/HPMA according to the general process of Example 10 described in the aforementioned PCT patent publication with the following monomer mixing weight ratio: S/EHMA/HPMA//MMA/HEMA/MAA=25/23.5/24//14.3/7.7/5.5. The waterborne dispersant had % solids of 39.97 and pH of 9.04.
Example 3
Synthesis of Polymer Dispersant 3A
Polymer Dispersant 3 was produced according to the macromonomer polymerization process described in U.S. Pat. No. 5,231,131. The process is described briefly as follows. The macromonomer MAA/MMA with monomer mixing weight ratio=19.8/44 was prepared according to the general process of Example 1 of the aforementioned U.S. Patent. The macromonomer was then polymerized in the presence of backbone monomers mixture BA/AA/MA according to the general process of Example 2 described in the aforementioned U.S. Patent with the following monomer mixing weight ratio: BA/AA/MA//MAA/MMA=31.4/6.2/31.4//8.9/22.0%. The waterborne dispersant had % solids of 30.45 and pH of 9.25.
Molecular Weight Data for Branched Copolymers in Examples













Macromonomer
Branched Copolymer












Mn
Mw
Mn
Mw





Polymer 1A
1000-1800
2200-3600
6500-8500
35000-80000


Polymer 2A
1000-1800
2200-3600
2900-3600
12000-16500


Polymer 3A
1300-2000
2600-6000
 8500-10500
24000-32000









Examples 1-3 and Comparative Example A
Description of Examples from Canola Greenhouse Trials
General Procedure for Preparing Insecticidal Compositions
For Examples 1, 2 and 3, 1.0 g of branched copolymer and 1.0 g of Compound 1 (HGW) were dissolved in 50 mL of 30 wt % ethanol/methylene chloride. The solvent was removed by rotary evaporation. Some of the residue (0.5 g) was mixed with 1 g of water for seed coating.
For Comparative Examples A, 1.01 g of Compound 1 was dissolved in 50 mL of 30 wt % ethanol/methylene chloride. The solvent was removed by rotary evaporation. Some of the residue (0.5 g) was mixed with 1 g of water for seed coating.
The compositions of Examples 1, 2, 3 and Comparative Example A were mixed with a 1:3 by weight mixture of the fungicide products MAXIM 4FS (40.3% fludioxonil, Syngenta AG) and APRON XL (33.3% mefenoxam, Syngenta AG), and (2) Acid Blue 1 (Simpsons (UK) Ltd), and then the resultant compositions were used to coat canola seeds at an application rate of 0.6 g of Compound 1, 0.067 mL of the fungicide mixture (1A) and 0.033 g of the colorant (2) per 100 g of canola seeds (100 g corresponding to about 23,400 seeds for Examples 1-9, and Comparative Example A). (“Canola” is a cultivar of the rapeseed species Brassica napus L. that produces an edible oil.)
The coated canola seeds were then evaluated for ability to provide Compound 1 to leaves developing from the seeds. Each treatment involved four pots to provide quadruple replication. Four coated canola seeds were planted in sterile Matapeake sand blend soil in each pot and then grown in a growth chamber (25° C., 18 h light, 6 h dark) for 18-20 days. Three plants in each pot were selected for sampling. From each of the three plants, the second leaf was cut at the stem. All three leaves collected from each pot were placed into one vial and then analyzed according to the general procedure described above for assaying anthranilic diamide concentration in leaves. The concentrations measured from leaves in each of the four pots (total of 12 leaves) were averaged to provide the values reported in Table 3.







TABLE 3







Uptake of Compound 1 in Canola










Uptake
Improvement


Example
(microgram/g of leaf)
vs Compound 1*





1
0.074
6.2


2
0.078
6.5


3
0.083
6.9


Comparative
0.012
1.0


Example A





*The “Improvement vs Compound 1” represents the amount of Compound 1 taken up by the leaves in the presence of component (b) divided by the amount of Compound 1 taken up in the absence of component (b).

## Claims

1. A composition comprising at least one compound selected from anthranilic diamides of Formula 1, N-oxides, and salts thereof,



wherein
X is N,
R1 is CH3, Cl, Br or F;
R2 is H, F, Cl, Br or —CN;
R3 is F, Cl, Br, C1-C4 haloalkyl or C1-C4 haloalkoxy;
R4a is H, C1-C4 alkyl, cyclopropylmethyl or 1-cyclopropylethyl;
R4b is H or CH3;
R5 is H, F, Cl or Br;
R6 is H, F, Cl or Br, and
further comprising a branched methacrylate copolymer segment comprising one or more polymer arms comprising a macromer polymerized from methyl methacrylate, methacrylic acid, and, optionally hydroxyethyl methacrylate, attached to a linear backbone segment wherein the linear backbone segment comprises comonomers selected from the group consisting of acrylates, methacrylates, styrenics, and mixtures thereof, and wherein the branched methacrylate copolymer segment further comprises hydrophilic groups,

and, wherein the hydrophilic groups comprise one or more of a —COOH, —OH, —NH2, an amide group, a vinyl group, a urethane group, an isocyanate group, or a blocked isocyanate group, and wherein the ratio of the branched methacrylate copolymer to the anthranilic diamide of Formula I is about 1:5 to about 4:1 by weight.

2. The composition of claim 1, wherein the at least one compound selected from anthranilic diamides of Formula 1, is selected from compounds of Formula 1 wherein X is N; R1 is CH3; R2 is Cl or —CN; R3 is Br; R4a is CH3; R4b is H; R5 is Cl; and R6 is H; and salts thereof.

3. The composition of claim 2, wherein R2 is Cl.

4. The composition of claim 2, wherein R2 is —CN.

5. The composition of claim 1, wherein the branched methacrylate copolymer is at least about 15% of the composition by weight.

6. The composition of claim 1, wherein the ratio of the branched methacrylate copolymer to the anthranilic diamide of Formula I is at least about 1:5 by weight.

7. The composition of claim 1, further comprising at least one fungicide or insecticide other than anthranilic diamide insecticides.

8. A geotropic propagule coated with an insecticidally effective amount of the composition of claim 1.

9. The geotropic propagule of claim 8, wherein the geotropic propagule is a seed.

10. The geotropic propagule of claim 9, wherein the seed is a seed of cotton, maize, soybean, rapeseed or rice.

11. A liquid composition consisting of about 5 to 80 weight % of the composition of claim 1 and about 20 to 95 weight % of a volatile aqueous liquid carrier.

12. A method for protecting a geotropic propagule and plant derived therefrom from a phytophagous insect pest, the method comprising coating the propagule with an insecticidally effective amount of the liquid composition of claim 11 and then evaporating the volatile aqueous liquid carrier of the composition.

13. The method of claim 12 wherein the insect pest is in a taxonomic order selected from Hemiptera and Lepidoptera.

14. The composition of claim 1, wherein the one or more polymer arms comprise at least two repeating monomer units and have a number average molecular weight (Mn) of at least about 1,000.

15. The composition of claim 1, wherein the one or more polymer arms are randomly distributed along the linear backbone segment, each branch segment being a polymer, copolymer, or oligomer of at least two repeating monomer units, which may be the same or different, wherein the branch segment is covalently attached to the linear backbone.