# Patent

## Title
Method for detecting pathogens attached to specific antibodies

## Abstract

The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.

## Background
This is a divisional of application Ser. No. 09/737,542, filed Dec. 14, 2000.


The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48) between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.


BACKGROUND OF THE INVENTION
Field of the Invention
The present invention relates to detecting the presence of pathogens trapped in an electric field of a fluidic device, particularly to pathogens attached to antibodies immobilized in the electric field, and more particularly to the use of impedance measurements for detecting the presence of pathogens attached to antibody—coated beads located in the electric field.
Dielectrophoresis (DEP) is currently utilized to collect or concentrate pathogens in an electric field of a fluidic device. Recently, interdigitated electrodes have been patterned on the surface of a fluidic channel for generation of a non-uniform electric field by applying a voltage across the electrodes and pathogens are trapped by the dielectrophoretic force.
One typical method of detecting pathogens is to detect whether or not they attach to specific antibodies. The antibodies are typically flourescently labeled and this increases in fluorescence is detected optically. Multiple pathogen detection has been accomplished by immobilizing antibodies on a surface, and then introducing pathogens in a fluid to the surface. The pathogen binds to the surface and then are detected by optical means.
Recently, impedance measurements across adjacent electrodes has been utilized to detect the presence of trapped pathogens, and such an approach has been described and claimed in co-pending application Ser. No. 09/738,927, filed Dec. 13, 2000, entitled “Using Impedance Measurements For Detecting Pathogeris Trapped In An Electric Field”, assigned to the same assignee.
The present invention expands the impedance measurement approach to detect the presence of pathogens attached to immobilized antibodies, and to the use of antibody-coated beads for enhancing the impedance change to amplify this impedance change signal. The present invention utilizes patterned interdigitated electrodes located on the surface of a fluidic channel with an AC or DC voltage is applied across the electrodes, antibodies are immobilized on the surface of the electrodes and pathogens flowing in a sample fluid therepast are attached to the immobilized antibodies causing a change in impedance between adjacent electrodes. This impedance change can be enhanced by providing antibody-coated beads that attach to the pathogens thereby causing a greater impedance change.
SUMMARY OF THE INVENTION
It is an object of the invention to detect pathogens attached antibodies in a fluidic flow channel.
A further object of the invention is to detect the attachment of pathogens to immobilized antibodies in an electric field by measuring impedance change between adjacent electrodes caused by the presence of the pathogens.
Another object of the invention is to detect the attachment of antibodies to pathogens using the change of impedance between two electrodes.
Another object of the invention is to provide a sensor to detect the presence of pathogens trapped in an electric field or attached to antibodies immobilized in the field by measurement of impedance changes between two patterned electrodes located in a fluidic channel.
Another object of the invention is to enhance an impedance change signal causes by pathogens being trapped in an electric field by providing antibody-coated beads that attach to the trapped pathogens causing a greater impedance change.
Another object of the invention is to detect pathogens trapped in an electric field by providing antibody coated beads which attach to the pathogens causing further change in the impedance between two electrodes, and moving the thus coated beads in an out of the signal region.
Another object of the invention is to provide a device for measuring impedance change in electrodes located in a fluidic channel and caused by trapped pathogens, and providing a second set of electrodes to act as a reference signal. Other objects and advantages of the present invention will become apparent from the following description and accompanying drawings. The present invention is directed to the use of impedance measurements to detect the presence of pathogen attached to antibodies, and more particular to pathogens attached to antibody coated beads. Basically, the present invention replaces the prior optical detection approach with the detection of the attachment of antibodies to pathogens using the change of impedance between two electrodes as a way of making a less expensive pathogen detection system. The detection system or sensor of this invention, may for example, use patterned intedigitated electrodes on the surface of a fluid channel, with an AC or DC voltage applied across the electrodes to produce a non-uniform electric field. Antibodies are immobilized on the surfaces of the electrodes and the impedance between the electrodes is measured. Following the passage of a sample fluid containing pathogens through the fluid channel and by the electrode surface, certain of the pathogen are attached to the immobilized antibodies, thereby causing a change in the impedance between the electrodes, which change of impedance provides a measurement by which the presence of the pathogens can be determined. To amplify the impedance change signal, antibody coated bead may be introduced which attach to the trapped pathogens causing a greater change in impedance between the electrodes. The pathogen sensor of this invention can be used, for example, in counter biological warfare detectors to detect the presence of pathogens, or in any antibody-based assay system.



BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying drawings, which are incorporated into a form a part of the disclosure, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.
FIG. 1 illustrates in cross-section a fluidic channel with patterned interdigitated electrodes formed on a surface of the channel with electrode fields shown across the various legs of the interdigitated electrodes.
FIG. 2 is an enlarged to view of the patterned interdigitated electrodes of FIG. 1 with an AC power supply connected across the two electrodes to produce the electric field illustrated in FIG. 1.
FIG. 3 illustrates only a pair of the electrode legs of FIGS. 1 and 2 for simplicity with antibodies immobilized on the surfaces of-the electrodes in accordance with the present invention.
FIG. 4 illustrates the electrodes of FIG. 3 with pathogens attached to certain of the immobilized antibodies.
FIG. 5, which is similar to FIG. 4, additionally illustrates the addition of antibody-coated beads which attach to the pathogens for amplifying the impedance change between the electrodes.
FIG. 6 illustrates an embodiment of an arrangement similar to FIG. 4, but with the addition of a pair of reference electrodes spaced via an insulator from the two electrodes of FIG. 4.
FIG. 7 schematically illustrates an impedance sensor mounted to the two electrodes of FIGS. 3-5.



DETAILED DESCRIPTION OF THE INVENTION
The present invention is directed to a method and apparatus (sensor) for detecting the presence of pathogens attached to antibodies, either immobilized or coated on beads, by the use of impedance measurements between electrodes. The invention can be utilized, for example, for detecting pathogens to determine whether or not the pathogens attached to specific antibodies, or merely to detect the presence of pathogens in a sample fluid flowing past immobilized antibodies. By the detection of the attachment of antibodies to pathogens using the change of impedance between two electrodes, or two adjacent legs of two multileg interdigitated electrodes, a less expensive pathogen detection system is provided compared to the previously utilized optical detection system, due to the reduction in instrumental costs.
Since antibodies can be immobilized on a surface, the present invention utilizes surfaces of two spaced electrodes or two adjacent legs of patterned interdigitated electrodes as a surface on which the antibodies are immobilized, as illustrated in FIG. 3 and described in detail hereinafter, the electrodes of FIG. 3 being two of the adjacent legs of the interdigitated electrodes of FIGS. 1 and 2. The pathogen streaming by the electrode surfaces attach to the immobilized antibodies, as seen in FIG. 4, and thus changes the impedance between the two electrodes, which is measured, as by the sensor of FIG. 7, to detect the presence of a pathogen. To amplify this impedance change signal, one or more beads coated with antibodies are introduced into the sample fluid flow, and the beads stick to the trapped pathogen as seen in FIG. 5. The coated beads further add to the change in impedance between the two electrodes. The coated bead or beads can be moved in and out of the signal region using either electrophoretic, dielectrophoretic, magnetic, or pressure flow modes so that the bead or beads could be recycled. Beads bound to pathogen could be eluted and the surface reactivated. Also, a second set of electrodes could be placed beneath the first set to act as a reference signal, as shown in FIG. 6. The sensor of FIG. 7 is shown operatively connected to two electrodes, such as shown in FIGS. 3-6, but may be expanded to be connected between two adjacent legs of the interdigitated electrodes of FIG. 2.
Referring now to the drawings, FIG. 1 illustrates a cross-section of a fluidic channel with patterned interdigitated electrodes located on a surface of the channel, and with FIG. 2 illustrating a top view of the interdigitated electrodes of FIG. 1. As shown, a fluid device 10 includes at least one fluidic channel or microchannel 11 having patterned on an interior surface thereof a pair of interdigitated electrodes indicated generally at 12 and 13, across each adjacent leg pair is formed an electric field 14. As seen in FIG. 2, each electrode 12 and 13 includes a base section or member 15 and 16 to which an AC power supply 17 is connected to produce the electric fields 14 of FIG. 1. The power supply 17 may be of a DC type. Extending from each of the base members 15 and 16 of electrodes 12 and 13 are a pair of fingers or members 18-19 and 20-21, respectively, with finger 18 located intermediate fingers 20 and 21, with finger 21 located intermediate fingers 18 and 19. Thus, each adjacent pair of fingers (20-18, 18-21, and 21-19) form a pair of spaced electrodes across which an electric field 14 is created by applied voltage from AC power supply 17. The electrodes can be located within the fluidic channel by use of spaced wires or suspended structures.
In the embodiments illustrated in FIGS. 3-5 and FIG. 6, only two electrodes are shown for simplicity of illustration, and such are illustrated as electrodes or electrode fingers 20 and 18 of FIGS. 1 and 2. In FIGS. 3-6, components corresponding to components of FIGS. 1 and 2 are given corresponding reference numeral. The electrodes can be straight, as shown, or can be curved, with either uniform or variable widths or thickness.
As shown in FIG. 3, antibodies 22 are immobilized on the surface of electrodes 20 and 18 and the exposed surface 23 of channel 11 between the electrodes. As sample fluid streams pass the immobilized antibodies 22 on electrodes 20 and 18, pathogens in the sample fluid attach to the immobilized antibodies 22 as shown by pathogen 24 in FIG. 4. An impedance measurement between electrodes 20 and 18 is made prior to the passage of the sample fluid across the electrodes and after passage of the sample fluid, and the change in the impedance caused by the trapped pathogen is determined and measured to detect the presence of the pathogen by the impedance sensor of FIG. 7.
To amplify the impedance change signal, one or more beads 25 coated with antibodies can be introduced to the signal area (electrodes 20-18) as shown in FIG. 5. The coated bead or beads 25 stick to the pathogen 24 and further add to the change in impedance between the electrodes 20 and 18. As pointed out above the bead or beads 25 can be moved in and out of the signal region using either electrophoretic, dielectrophoretic, magnetic, or pressure flow mode, known in the art, so that the beads could be recycled. Also, the beads 25 could be eluted and the surface reactivated.
As shown in FIG. 6, a pair of electrodes 26 and 27, electrically connected to power supply 17 are located beneath the electrodes 20 and 18 to act as a reference signal. The reference electrodes 26 and 27, in the FIG. 6 embodiment are separated from electrodes 20 and 18 by a thin ( ̃1 μm) insulator layer 28. If reference electrodes, as in FIG. 6 is utilized with interdigitated electrodes 12 and 13 of FIG. 1 and 2, a reference electrode would be located beneath each of the electrode legs 18-21.
FIG. 7, illustrates an embodiment of an impendance sensor, which as illustrated is operatively connected only to electrodes 20 and 18 for simplicity of illustration. It is to be recognized that the sensor embodiment of FIG. 7 would need be modified to sense each adjacent pair of the interdigitated electrode legs 18-21 of FIGS. 1 and 2, and such modification is within the skill of the art.
As shown in FIG. 7, this embodiment comprises electrodes 18 and 20 located in a microchannel device 10′, with a 0° signal generator 30 electrically connected to electrode 18 and a current sensor 31 electrically connected to electrode 20. A pair of amplifiers 32 and 33 are connected in parallel to current sensor 31, with mixers 34 and 35 operatively connected to amplifiers 32 and 33, which measure the impedance (z) in-phase, indicated at 36, and out-of-phase indicated at 37, of the components of the device. A 90° signal generator 38 is electrically connected to the mixer 35, with signal generator 30 electrically connected to mixer 34. Signal generators 30 and 38 drive dielectrophoretic device electrodes 18 and 20. Collected particles cause a change in the device impedance, as described above, and the output of the current sensor 31. Amplifiers 32 and 33 and mixers 34 and 35 measure the in-phase 36 and out-of-phase 37 components of the devices complex impedance.
It has thus been shown that the present invention enables the use of impedance measurements to detect the presence of pathogens attached to immobilized antibodies and/or beads coated with antibodies. The impedance change pathogen detection approach of the present invention provides a simplified and less expensive system than the prior utilized optical detection systems. The sensor of this invention can be effectively utilized in antibody-based assay system as well as for detecting the presents of pathogens in counter biological warfare detectors.
While particular embodiments of the present invention have been illustrated and described to exemplify and teach the principles of the invention. Modifications and changes may become apparent to those skilled in the art, and it is intended that the invention be limited only by the scope of the appended claims.

## Claims

1. A method for detecting pathogens attached to specific antibodies, comprising:
providing a fluidic channel with at least one pair of spaced electrodes, 
providing an AC or DC power source to produce an electric field across the at least one pair of spaced electrodes, 
depositing antibodies on the spaced electrodes, 
providing antibody coated beads, 
directing a sample fluid containing pathogens and the antibody coated beads past the spaced electrodes, 
allowing the pathogens to bind to the antibodies that are deposited on the spaced electrodes, 
allowing the antibody coated beads to stick to the pathogens that are bound to the antibodies that are deposited on the spaced electrodes, 
measuring the impedance between the spaced electrodes prior to said sample fluid containing pathogens and the antibody coated beads being directed past the spaced electrodes, and 
determining the presence of pathogens attached to the antibodies by measuring change in impedance between said pair of spaced electrodes with the antibody coated beads amplifying the change in impedance.

2. The method of claim 1, wherein the at least one pair of electrodes comprises a plurality of adjacent spaced pairs of electrodes.

3. The method of claim 1, wherein the at least one pair of spaced electrodes is formed on surfaces of the fluidic channel.

4. The method of claim 3, wherein forming the spaced electrodes on the surfaces of the fluidic channel is carried out by depositing an interdigitated electrode on the surfaces whereby adjacent fingers of the interdigitated electrode form at least one pair of spaced electrodes.

5. The method of claim 4, additionally including forming the interdigitated electrode to produce a plurality of sets of adjacent pairs of electrodes.

6. The method of claim 1, additionally including providing an impedance sensor assembly operatively connected to at least one pair of spaced electrodes for measuring the impedance between the spaced electrodes, an including impedance readout means.

7. The method of claim 6, additionally includes providing reference electrodes in insulated and spaced relation to the at least one pair of spaced electrodes, and electrically connecting the impedance sensor assembly to the reference electrodes.

8. The method of claim 6, wherein providing the impedance sensor assembly is carried out by at least amplifiers and mixers to measure the in-phase and out-of-phase impedance.