Exhibit 8

Tsakos Energy Navigation Ltd. and Subsidiaries

As of
31 March 2012



|                                 |  |                         
|-------------------------------- |  | ------------------------
|Company                          |  | Country of Incorporation
|Oak Shipping Co. Ltd.            |  | Liberia                 
|Avra Trading Co. Ltd.            |  | Liberia                 
|Romeo Shipping Company Limited   |  | Liberia                 
|Figaro Shipping Company Limited  |  | Liberia                 
|Freeport Dominion S.A.           |  | Panama                  
|Freeport Faith S.A.              |  | Panama                  
|Ergo Glory S.A.                  |  | Panama                  
|World Excellence S.A.            |  | Panama                  
|Apollo Honour S.A.               |  | Panama                  
|Apollo Glory S.A.                |  | Panama                  
|Apollo Excellence S.A.           |  | Panama                  
|Activity Excellence S.A.         |  | Panama                  
|Worldwide Overseas S.A.          |  | Panama                  
|Sea Mentor S.A.                  |  | Panama                  
|Sea Polar S.A.                   |  | Panama                  
|Fortune Faith S.A.               |  | Panama                  
|Victory Faith S.A.               |  | Panama                  
|Victory Spirit S.A.              |  | Panama                  
|Victory Mare S.A.                |  | Panama                  
|Universal Reserve S.A.           |  | Panama                  
|Sea Countess S.A.                |  | Panama                  
|Global Triumph S.A.              |  | Panama                  
|Fairsea Enterprises S.A.         |  | Panama                  
|Freeport Champion S.A.           |  | Panama                  
|Prosperity Faith S.A.            |  | Panama                  
|Prosperity Success S.A.          |  | Panama                  
|Mercury Emerald S.A.             |  | Panama                  
|Powerful Shipping S.A.           |  | Panama                  
|Sea Optima S.A.                  |  | Panama                  
|Shipping Celebrity S.A.          |  | Panama                  
|Southport Marine S.A.            |  | Panama                  
|Southport Maritime S.A.          |  | Panama                  
|Sea Pioneer S.A.                 |  | Panama                  
|Sea Celebrity S.A.               |  | Panama                  
|Gladiator Shipping Services S.A. |  | Panama                  
|Southport Navigation S.A.        |  | Panama                  
|Triton Success S.A.              |  | Panama                  
|Triton Triumph S.A.              |  | Panama                  
|Optima Maritime S.A.             |  | Panama                  
|Optima United S.A.               |  | Panama                  
|Bayswater Trading Co. Ltd.       |  | Liberia                 
|Kerry Trading Company Limited    |  | Liberia                 
|Marine Velvet S.A.               |  | Panama                  
|Medway Sea S.A.                  |  | Panama                  
|Sayers Shipping Corp.            |  | Liberia                 
|Maynard Shipping Corp.           |  | Liberia                 
|Payton Shipping Corp.            |  | Liberia                 
|Dorsett Shipping Corp.           |  | Liberia                 
|Pearsall Shipping Corporation    |  | Liberia                 
|Angleton Shipping Corporation    |  | Liberia                 
|Mare Success S.A.                |  | Panama