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ARS Home Midwest Area Madison, Wisconsin Vegetable Crops Research Docs





- John Bamberg
- Paul Bethke
- Johanne Brunet
- Dennis Halterman
- Michael Havey
- Shelley Jansky
- Philipp Simon
- David Spooner
- Yiqun Weng
- David Willis
- IFAFS

Simon: Pubs: 99plan0032headline bar Plant Cell Reports (1999) 19: 32-36 J. M. Myers - P. W. Simon Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time Received: 24 September 1998 / Revision received: 27 January 1999 / Accepted: 26 February 1999 Abstract  A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 ?M 2,4-D and maintained on the same basal medium with 4.7 ?M picloram+0.49 ?M 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 ?M picloram+13.3 ?M BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation. Key words  Allium sativum •• Benzyladenine •• Picloram •• Thidiazuron •• 2,4-D •• 2iP   Communicated by C.F. Quiros J.M. Myers •• P. W. Simon (envelope)USDA-ARS Vegetable Crops Research, Department ofHorticulture, 1575 Linden Drive, University ofWisconsin-Madison, Madison, WI 53706, USAe-mail: psimon@facstaff.wisc.eduFax: +608-262-4743


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