# Patent

## Title
Biohazard sensing system and methods thereof

## Abstract

A sensing system for a target includes a member with an embedded charge, at least one input electrode, at least one output electrode, at least one common electrode, one or more probes, an input system, and an output monitoring system. The input and output electrodes are spaced from and on substantially opposing sides of the member from the common electrode. At least one of the member and the input and output electrodes is movable with respect to the other. The probes which engage with the target hazardous substance are connected to the at least one of the member and the input and output electrodes which is movable with respect to the other. The input system is coupled between the at least one input electrode and the at least one output electrode and provides an input signal. The output monitoring system is coupled between the at least one output electrode and the at least one common electrode and detects a change in an output signal when the target engages with the movable member or electrode.

## Background
This application is a continuation-in-part application of U.S. patent application Ser. No. 10/238,438 filed Sep. 9, 2002 now U.S. Pat. No. 6,717,488 which claims the benefit of U.S. Provisional Patent Application Ser. No. 60/318,914 filed Sep. 13, 2001 which are both herein incorporated by reference in their entirety.


FIELD OF THE INVENTION
The present invention relates to a biohazard sensing system which uses a resonator that has a member with embedded fixed charge and methods thereof.
BACKGROUND OF THE INVENTION
A high Q mechanical resonator is a device which is used to pass frequencies which are very close to the resonant frequency of the mechanical resonator. As disclosed in “Large Jobs for Little Devices” Stephen Cass, IEEE SPECTRUM, January 2001, pp.72-73, which is herein incorporated by reference in its entirety, these high Q mechanical resonators are being operated in the 100+MHz range.
Unfortunately, there are some problems with the existing high Q mechanical resonators. For example, the performance of these prior high Q resonators has been limited by stiction forces. Additionally, the upper frequency range of these high Q resonators is low restricting their use for some applications. Further these high Q resonators are difficult to fabricate and have a relatively large mass.
Recently, it has been demonstrated that individuals or small groups of individuals can cause great harm to society by premeditated acts of terrorism. One such possibility is the deliberate introduction of a bio hazardous material into the local environment. Therefore, in order to provide maximum self-protection, societies must develop rapid identification capabilities for biohazard substances.
The need to accurately identify the presence of a substance or other thing is applicable in a wide variety of other environments. For example, in an agricultural environment, farmers monitor for the presence of insects, bacteria, fungus, and/or spores which may damage their crops. Since identification of these things is difficult, the farmers often have to take preventive measures, such as the application of certain chemicals, prematurely and sometimes unnecessarily to prevent the chance of losing some or all of their crops.
SUMMARY OF THE INVENTION
A sensing system in accordance with an embodiment of the present invention includes a member with an embedded charge, at least one input electrode, at least one output electrode, at least one common electrode, one or more probes, an input system, and an output monitoring system. The input and output electrodes are spaced from and on substantially opposing sides of the member from the common electrode. At least one of the member and the input and output electrodes is movable with respect to the other. The probes obtain information about a presence of a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, are connected to the at least one of the member and the input and output electrodes which is movable with respect to the other. The input system is coupled between the at least one input electrode and the at least one output electrode and provides an input signal. The output monitoring system is coupled between the at least one output electrode and the at least one common electrode and detects a change in an output signal when one of the hazardous substance, the insect, the bacteria, the fungus, and the spore bind with the one or more probes. The common electrodes may be divided into two separate electrodes in order to facilitate independent bias application, for example to tune the system.
A method in accordance with an embodiment of the present invention includes providing a member with an embedded charge, providing at least one input electrode, providing at least one output electrode, and providing at least one common electrode. The input and output electrodes are spaced from and on substantially opposing sides of the member from the common electrode. At least one of the member and the input and output electrodes are movable with respect to the other. One or more probes are provided which obtain information about the presence of a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. The probes are connected to the at least one of the member and the input and output electrodes which is movable with respect to the other. An input system which provides an input signal is coupled between the at least one input electrode and the at least one output electrode. An output monitoring system is coupled between the at least one output electrode and the at least one common electrode and detects a change in an output signal when the probes.
A method for passing a signal with a resonator in accordance with an embodiment of the present invention includes receiving an input signal at a resonant frequency for the resonator. A first varying signal is applied on at least a first pair electrodes in response to the received input signal. Each of the first pair of electrodes is spaced from and on substantially opposing sides of a member with an embedded charge. The member oscillates in response to the applied varying signal on the first pair of electrodes. An output signal is transmitted based on a second varying signal which is induced on the second pair of electrodes in response to the oscillation of the member. Probes which obtain information about a presence of a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, are located on one of the member and one of the first pair of electrodes are placed in an environment to be monitored. The presence of the target is signaled when a change in the output signal is detected.
The present invention provides an rf-MEMS resonator which avoids many of the problems with existing high Q resonators. For example, the present invention avoids the prior problem with stiction. Additionally, the present invention is much simpler to fabricate, can operate at higher frequencies, is more robust, and has a higher reliability than prior resonators. The present invention can be used in a variety of different applications and can be used in a number of different products, including cell phone filters, wireless filters in general, high frequency filter networks, biohazard hazard monitor, gas monitor, insect monitor, bacteria monitor, fungus monitor, spore monitor, and prion monitor.
Furthermore, the present invention is electronic in nature and can be integrated with standard semiconductor integrated circuits. Neither a light-source, such as a laser nor a light detector, such as a CCD, is required. Since the input interrogation signal is electronic, the present invention does not depend on a mechanical input such as a mechanical vibration.
The present invention also provides an effective, simple and inexpensive detection system with a resonator for detecting a presence of a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. The resonator has an embedded charge layer totally isolated and sealed from the ambient environment. As a result, no contamination can damage, harm or otherwise interfere with the intended function of detection system.
In an agricultural environment, the present invention provides an accurate system and method for detecting the presence of one or more hazardous substances, insects, bacteria, fungi, and spores. As a result, farmers can take measures to protect their crops when one or more of the hazardous substances, insects, bacteria, fungi, spores, and/or prions are detected. Additionally, with the present invention the use of some pesticides and other chemicals is reduced because the farmers only need to use them when a problem is detected.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1-11 are side, cross-sectional views of a method of making a resonator in accordance with an embodiment of the present invention;
FIG. 12 is a top, cross-sectional view of a resonator with an output electrode with a circular shape and an input electrode which is spaced from and surrounds the output electrode in accordance with another embodiment;
FIG. 13 is a side, cross-sectional view of the resonator shown in FIG. 11 with an input and an output;
FIG. 14 is side, cross-sectional view of a resonator with a member with embedded charge secured at substantially opposing ends in accordance with another embodiment of the present invention;
FIG. 15 is a side, cross-sectional view of a resonator in a transceiver with a member with embedded charge secured at one end in accordance with another embodiment of the present invention;
FIG. 16 is a perspective view of a portion of the resonator shown in FIG. 1;
FIG. 17 is a perspective view of a portion of the resonator shown in FIG. 15;
FIG. 18 is a perspective view of a portion of a resonator in accordance with another embodiment of the present invention;
FIG. 19 is a perspective view of a portion of a resonator in accordance with another embodiment of the present invention with leads to an input, output, and tuning bias on the member;
FIG. 20 is a perspective view of a portion of a resonator in accordance with another embodiment of the present invention with leads to inputs, an output, and a common;
FIG. 21 is a perspective view of a portion of a resonator with a pair of input electrodes, an output electrode, and a common electrode in accordance with another embodiment of the present invention;
FIG. 22 is a side, cross-sectional view of the resonator with two pairs of corresponding input electrodes and a corresponding pair of output electrodes in accordance with another embodiment;
FIG. 23 is a side, cross-sectional view of a resonator with corresponding input and output electrodes on each side of the member in accordance with another embodiment;
FIG. 24 is a side, cross-sectional view of a resonator with an input electrode, an output electrode, and a common electrode in accordance with another embodiment;
FIG. 25 is a side, cross-sectional view of a bio sensor system with a resonator with an input electrode, an output electrode, and a common electrode in accordance with another embodiment;
FIG. 26 is a graph of an initial and post target binding output voltage or signal vs. and initial or interrogation frequency;
FIG. 27 is a perspective view of a portion of a bio sensor system with a resonator with a pair of input electrodes, an output electrode, and a common electrode in accordance with another embodiment of the present invention;
FIG. 28 is another graph of an initial and post target binding output voltage or signal vs. and initial or interrogation frequency; and
FIG. 29 is a side, cross-sectional view of a bio system with a resonator with an input electrode, an output electrode, and a common electrode in accordance with another embodiment for detecting a target, such as an insect, bacteria, fungus, spore, or prion.



DETAILED DESCRIPTION
A method for making a resonator 10(1) in accordance with an embodiment of the present invention is illustrated in FIGS. 1-11, 13, and 16 In this particular embodiment, the resonator 10(1) includes a chamber 12, a member or resonator membrane 14(1) with an embedded charge 15, two pairs of input electrodes 16(1)-16(2) and 18(1)-18(2) and one pair of output electrodes 20(1)-20(2), although the resonator can comprise other numbers and types of components. The present invention provides high Q rf-MEMS resonators 10(1)-10(12) which are not limited by stiction, can be made very small, can operate in a single mode, can operate at very high frequencies, and can be easily integrated with a variety of different types of semiconductor devices. The present invention also provides an effective, simple and inexpensive detection system with a resonator for things, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion.
Referring to FIGS. 11 and 13-24, resonators 10(1)-10(12) in accordance with embodiments of the present invention are illustrated. Elements in FIGS. 12, 14-25, 27, and 29 which are identical to those described with reference to FIGS. 1-11 and 13, have like numerals. These like elements are identical except as described herein.
In these particular embodiments, each of the resonators 10(1)-10(12) has a high Q, although other variations are possible. Q is defined as the center frequency divided by the bandwidth. In this particular embodiment, the resonators 10(1)-10(12) have a Q over about 1000, although the value of Q can vary.
Each of the resonators 10(1)-10(12) includes a chamber 12 which is made of a variety of layers, although other types of supporting structures for the member 14(1), 14(2), or 14(3) and the electrodes 16(1), 16(2), 18(1), 18(2), 20(1) and 20(2) in other configurations and shapes and with other numbers of layers and made of other materials can be used. The size of the chamber 12 can vary as required by the particular application. For ease of illustration, portions of the chamber 12 are not shown in FIGS. 16-21.
Each of the resonators 10(1)-10(12) also includes a member 14(1), 14(2) and 14(3) with an embedded charge 15. Referring to FIGS. 13, 14, 16, 18, and 21-25, 27, and 29, the member 14(1) comprises a pair of layers 22(1) and 22(2) of dielectric material, such as silicon oxide, silicon dioxide, silicon nitride, aluminum oxide, tantalum oxide, tantalum pentoxide, titanium oxide, titanium dioxide, barium strontium titanium oxide, or a material with residual polarization, such as an electret, although other types of materials could be used. The layers 22(1) and 22(2) are seated against each other along an interface 24 were the embedded charge 15 is stored, although other numbers of layers could be used and other types of members which can hold an embedded charge 15 could be used. Referring to FIGS. 15 and 17, the member 14(2) comprises a single layer 26 of dielectric material, such as such as silicon oxide, silicon dioxide, silicon nitride, aluminum oxide, tantalum oxide, tantalum pentoxide, titanium oxide, titanium dioxide, barium strontium titanium oxide, in which the embedded charge 15 is held. Referring to FIGS. 19 and 20, the embedded charge in the member 14(3) is a charged floating conductor. The member 14(3) comprises a single layer 27 of dielectric material, such as such as silicon oxide, silicon dioxide, silicon nitride, aluminum oxide, tantalum oxide, tantalum pentoxide, titanium oxide, titanium dioxide, barium strontium titanium oxide, with an embedded charge 15 that is a charged floating conductor.
Referring to FIGS. 13, 14, and 16 the member 14(1) is connected at one end 28(1) to an inner wall of the chamber 12, extends across the chamber 12, and has another end 28(2) which is connected on a substantially opposing side to another inner wall of the chamber 12, although other configurations and other types of members can be used can be used. In FIG. 16, the chamber 12 is not shown for ease of illustration, however the ends which are fixed are designated.
Referring to FIGS. 21-25, 27, and 29, the member 14(1) is secured on the layer of a layer of insulating material 38 and thus is fixed. In these particular embodiments, the moving or resonating part of the resonators 10(6)-10(11) are the electrodes 16(2), 18(2), and/or 20(2) which are embedded in the insulating layer 50 or other layer, although other arrangements are possible, such as attaching the electrodes 16(2), 18(2), and/or 20(2) to the insulating layer 50 or another layer or burying one or more of the electrodes 16(2), 18(2), and/or 20(2) in the insulating layer 50 or another layer. Although the spacing of the member 14(1) between the electrodes 16(1), 17, 18(1), and/or 20(1) and the member 14(1) is greater than the spacing between the electrodes 16(2), 18(2), and/or 20(2) and the member 14(1), the spacing is arranged so that the member 14(1) is electrically centered in the resonators 10(6)-10(7), 10(10), and 10(11) when the permitivities of the layer 38 and the permitivity of the air or other gas or fluid in the chamber 12 are taken into account or other arrangement if desired.
Referring to FIGS. 15 and 17, the member 14(2) is connected at one end 30(1) to an inner wall of the chamber 12, extends across the chamber 12, and has another end 30(2) which is free and is spaced from another inner wall of the chamber 12, although other configurations can be used and other types of members can be used.
Referring to FIGS. 18-20, other variations for connecting the member 14(1), 14(2), and 14(3) are shown which enable the resonator to operate at a higher frequency range above about 100 MHz. Referring to FIG. 18, the member 14(1) is connected along an elongated side 23(1) to an inner wall of the chamber 12 and extends out into the chamber 12 and has another opposing elongated side 23(2) that is free and is spaced from another inner wall of the chamber 12 and the ends 28(1) and 28(2) of the member 14(1) are free, although other configurations can be used and other types of members can be used. Referring to FIGS. 19-20, the member 14(3) is also connected along an elongated side 27(1) to an inner wall of the chamber 12 and extends out into the chamber 12 and has another opposing elongated side 27(2) that is free and is spaced from another inner wall of the chamber 12 and the ends 31(1) and 31(2) of the member 14(1) are free, although other configurations can be used and other types of members can be used.
Referring to FIGS. 13, 16, 19, 20, and 22 the resonators 10(1), 10(5), and 10(7) each include two pairs of input electrodes 16(1)-16(2) and 18(1)18(2) and one pair of output electrodes 20(1)-20(2), although other combinations of pairs electrodes could be used, such as the resonators 10(2), 10(3), and 10(8) with one pair of input electrodes 16(1)-16(2) and one pair of output electrodes 20(1)-20(2) as shown in FIGS. 14, 15, and 17, resonators 10(4), 10(6), and 10(11) with input electrodes 16(2) and 18(2), an output electrode 20(2), and a common electrode 17 as shown in FIGS. 18, 21, and 27, and resonators 10(9) and 10 (10) with input electrode 16(2), an output electrode 20(2), and a common electrode 17 as shown in FIG. 24. The pairs of input electrodes 16(1)-16(2) and 18(1)-18(2), the pair of output electrodes 20(1)-20(2), and the common electrode 17 in FIGS. 13-24 are located in the inner walls of the chamber 12, although the input electrodes 16(1)-16(2) and 18(1)-18(2), the output electrodes 20(1)-20(2), and the common electrode 17 could be positioned in other locations, such as buried within the walls of the chamber 12. Each electrode 16(2), 18(2), and 20(2) in each pair is spaced from and located on a substantially opposing sides of the member 14(1) or member 14(2) from the other electrode 16(1), 18(1), and 20(1) in the pair or form the common electrode 17. By way of example only, the distance between each of the pairs of electrodes 16(1)-16(2), 18(1)-18(2), and 20(1)-20(2) or 16(2), 18(2), and/or 20(2) and common electrode 17 is about 0.2 to 2.0 microns, although this distance can vary. Common electrode 17 may be a direct connection to ground, although other configurations can be used, such as coupling to a common reference level.
Referring to FIGS. 13, 16, 19, 20, and 22, the two pairs of input electrodes 16(1)-16(2) and 18(1)-18(2) are each adjacent opposing ends 28(1) and 28(2) or 31(1) and 31(2) of the member 14(1) or 14(3) and are located on opposing sides of the pair of output electrodes 20(1)-20(2), although other configurations could be used. For example, the resonators 10(1), 10(5), and 10(9) could have one pair of output electrodes 20(1)-20(2) with a substantially circular shape surrounded by one pair of input electrodes 16(1)-16(2) having a substantially annulus shape as shown in FIG. 12, although the input and output electrodes could have other shapes or be in an opposite orientation. This one pair of input electrodes 16(1)-16(2) having an annulus shape would replace the two pairs of input electrodes 16(1)-16(2) and 18(1)-18(2) shown in FIGS. 13, 16, 19, 20, and 22
Referring to FIGS. 14 and 23, one pair of input electrodes 16(1)-16(2) are positioned adjacent one end 28(1) of the member 14(1) and the other pair of electrodes 20(1)-20(2) are adjacent the other end 28(2) of the member 14(1), although other configurations could be used.
Referring to FIGS. 15 and 17, one pair of input electrodes 16(1)-16(2) are positioned adjacent one end 28(1) of the member 14(2) and the other pair of electrodes 20(1)-20(2) are adjacent the other end 28(2) of the member 14(2), although other configurations could be used.
Referring to FIGS. 18, 21, and 27, input electrodes 16(2) and 18(2) are each adjacent opposing ends 28(1) and 28(2) of the member 14(1) and are located on opposing sides of the output electrodes 20(2) and a common electrode 17 is on the opposing side of the member 14(1) from electrodes 16(2), 18(2), and 20(2), although other configurations could be used.
Referring to FIGS. 24, 25, and 29, input electrode 16(2) and output electrode 20(2) are each adjacent opposing ends 28(1) and 28(2) of the member 14(1) and a common electrode 17 is on the opposing side of the member 14(1) from electrodes 16(2) and 20(2), although other configurations could be used.
Referring to FIGS. 13, 20, and 22, an input lead 52, such as an antenna, is coupled to the input electrodes 16(2) and 20(2), although other types of input devices could be used. An output lead 54 is coupled to the output electrode 20(2), and a common, such as ground, is coupled to the input electrodes 16(1) and 18(1) and to output electrode 20(1), although other common reference levels could be used.
Referring to FIGS. 14 and 15, an input lead 52, such as an antenna, is coupled to the input electrode 16(2), although other types of input devices could be used. An output lead 54 is coupled to the output electrode 20(2), and a common, such as ground, is coupled to the input electrode 16(1) and to output electrode 20(1), although other common reference levels could be used.
Referring to FIG. 18, an input lead 52, such as an antenna, is coupled to the input electrodes 16(2) and 18(2), although other types of input devices could be used. An output lead 54 is coupled to the output electrode 20(2), and a common, such as ground, is coupled to the common electrode 17, although other common reference levels could be used.
Referring to FIG. 19, a tuning bias input 51 (1) and 51(2) is coupled to input electrodes 16(1) and 16(2) to tune the resonator 10(5) to a particular frequency. The other resonators 10(1)-10(4) can be configured to apply a tuning bias to tune the resonator to a particular frequency. An input lead 52(1) and 52(2) is coupled across the input electrodes 18(1) and 18(2) and an output lead 54(1) and 54(2) is coupled across the output electrodes 20(1) and 20(2), although other configurations can be used.
Referring to FIGS. 25 and 29, an input voltage 105 is coupled between the input electrode 16(2) and the common electrode 17. An output voltage 107 is coupled between the input electrode 18(2) and the common electrode 17, although other types of inputs and/or outputs can be coupled between the input electrode 16(2) and the common electrode 17 and between the input electrode 18(2) and the common electrode 17.
Referring to FIGS. 21 and 27, an input lead 52, such as an antenna, is coupled to the input electrodes 16(2) and 18(2), although other types of input devices could be used. An output lead 54 is coupled to the output electrode 20(2), and a common, such as ground, is coupled to the common electrode 17, although other common reference levels could be used.
A method for making a resonator 10(1) in accordance with an embodiment of the present invention in accordance with an embodiment will be described with reference to FIGS. 1-11, 13, and 16. Referring more specifically to FIG. 1, a layer 32 of insulating material, such as SiO2 is provided, although other types of materials and other numbers of layers could be used. For example, the layer 32 of insulating material could be deposited on a substrate which acts as the base.
Referring to FIG. 2, three openings or trenches 34(1)-34(3) are patterned and etched into the layer 32 of insulating material and then a conductive material 36, such as Cu, is deposited in each of the openings 34(1)-34(3) to form electrodes 16(1), 18(1), and 20(1), although other types of conductive materials could be used, the number of openings etched can vary depending on the number of electrodes desired, and other techniques for forming the electrodes can be used. The deposited conductive material 36 may be planarized.
Referring to FIG. 3, a layer 38 of insulating material, such as SiO2, may be deposited on a portion of the conductive material 36 in the openings 34(1)-34(3) and on a portion of the exposed layer 32 of insulating material. Next, an opening or trench 40 is patterned and etched in the layer 38 of insulating material which extends to a portion of the conductive material 36 in the openings 34(1)-34(3) and on a portion of the exposed layer 32 of insulating material, although the opening 40 can extend to other depths, such as a depth where the electrodes 16(1), 18(1), and 20(1) would still be covered by a portion of the layer 38 of insulating material or by another insulating layer or layers.
Referring to FIG. 4, a layer 42 of sacrificial material, such as polysilicon, is deposited in the opening 40 in the layer 38 of insulating material, although other types of sacrificial materials could be used, such as polymers. The layer 42 of sacrificial material may be planarized.
Referring to FIG. 5, a layer 22(1) of insulating material, such as a layer of silicon oxide, is deposited on the layer 42 of sacrificial material and a portion of layer 38 of insulating material which is exposed. Another layer of insulating material 22(2), such as a layer of silicon nitride, is deposited on the layer 22(1) of insulating material to form the member 14(1). An embedded charge 15 can be held at an interface 24 between the layers 22(1) and 22(2) of insulating material. Although two layers 22(1) and 22(2) are shown, the member 14(1) can be comprised of other numbers of layers, such as one layer 26 as shown in FIGS. 15 and 17, one layer 27 with embedded charge 15 which is a charged floating conductor as shown in FIGS. 19 and 20, or three or more layers. In a member with multiple layers, the embedded charge 15 can be held at one or more of the interfaces between the multiple layers.
Referring to FIG. 6, electrons are injected into the member 14(1) with an electron gun to deposit an embedded charge 15, although other techniques for storing embedded charge 15 in the member 14(1) can also be used, such as applying an electrical bias across the member 14(1) sufficient to cause electron injection, ballistic injection, polarization, and high field injection. The same techniques can be used to store an embedded charge 15 in the member 14(2) shown in FIGS. 15 and 17 and a charged floating conductor as the embedded charge 15 in the member 14(3) in FIGS. 19 and 20.
Referring to FIG. 7, a layer 44 of insulating material, such as SiO2, is deposited on the member 14(1), although other types of insulating materials could be used. Next, an opening or trench 46 is patterned and etched in the layer 44 of insulating material which extends to member 14(1).
Referring to FIG. 8, a layer 48 of sacrificial material, such as polysilicon, is deposited in the opening 46 in the layer 44 of insulating material, although other types of sacrificial materials could be used. The layer 48 of sacrificial material may be planarized.
Referring to FIG. 9, a layer 49 of conductive material, such as Cu, is deposited on the layer 48 of sacrificial material and on the exposed portion of the layer 44 of insulating material, although other types of conductive materials could be used. The layer 49 of conductive material is patterned and etched to form electrodes 16(2), 18(2), and 20(2).
Referring to FIG. 10, a layer 50 of insulating material, such as SiO2, is deposited over the remaining portion of the layer of conductive material 49 which forms electrodes 16(2), 18(2), and 20(2). The layer 50 of insulating material encapsulates and supports the electrodes 16(2), 18(2), and 20(2).
Referring to FIG. 11, an access hole or holes (not shown) to the layers 42 and 48 of sacrificial material are formed and the layers 42 and 48 of sacrificial material are removed through the access hole or holes to form the chamber 12, although other techniques for removing the layers 42 and 48 of sacrificial material can be used. The access hole or holes are closed and the chamber 12 can be sealed in a vacuum or filled with one or more gases depending on the particular application. Although not shown, suitable vias are made to electrodes 16(1), 16(2), 17, 18(1), 18(2), 20(1), and 20(2) for the different resonators 10(1)-10(5) for providing access for electrical contact, although other techniques for providing electrical coupling to electrodes 16(1), 16(2), 1718(1), 18(2), 20(1), and 20(2) could be used.
The method for making the resonator 10(2) shown in FIG. 14 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layer 32 of insulating material shown in FIG. 2 is patterned and etched to form two openings which are filled with the conductive material 36 to form the two electrodes 16(1) and 20(1). Additionally, the layer 49 of conducting material shown in FIG. 9 is patterned and etched to form the two electrodes 16(2) and 20(2).
The method for making the resonator 10(3) shown in FIGS. 15 and 17 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layer 32 of insulating material shown in FIG. 2 is patterned and etched to form two openings which are filled with the conductive material 36 to form the two electrodes 16(1) and 20(1). Additionally, in this particular embodiment only one layer 26 of insulating material, such as electret, is deposited in FIG. 5 to from the member 14(2) and the one layer 26 of insulating material is patterned and etched to remove a portion of the member 14(2) which extends over to a portion of the layer 38 of insulating material. As a result, the resulting member 14(2) which is formed is only connected at one end 30(1) to the chamber 12 and has an opposing end 30(2) which is spaced from an inner wall of the chamber 12. Further, in this particular embodiment the layer 49 of conducting material shown in FIG. 9 is patterned and etched to form the two electrodes 16(2) and 20(2).
The method for making the resonator 10(4) shown in FIG. 18 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layer 32 of insulating material shown in FIG. 2 is not patterned and etched. The deposited layer of conductive material 36 is not patterned or etched and forms the common electrode 17, although other arrangements can be used, such as starting with a substrate made of a conductive material which acts as the common electrode 17.
The method for making the resonator 10(5) shown in FIGS. 19 and 20 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layers of insulating material 22(1) and 22(2) which comprise member 14(1) are formed to have ends 28(1) and 28(2) free and spaced from the inner wall of the chamber 12, elongated side 23(1) of the member 14(1) is secured in the inner wall of the chamber 12, and elongated side 23(2) is free and spaced form the inner wall of the chamber 12, although other configurations could be used.
The method for making the resonator 10(6) shown in FIG. 21 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the deposited layer of conductive material 36 is not patterned or etched and forms the common electrode 17, although other arrangements can be used, such as starting with a substrate made of a conductive material which acts as the common electrode 17. Additionally, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Further, the layer 50 of insulating material shown in FIG. 10 is formed to have ends 59(1) and 59(2) and elongated side 61(1) free, while elongated side 61(2) of layer 50 is fixed so that the electrodes 16(2), 18(2), and 20(2) and layer 50 can move and resonate.
The method for making the resonator 10(7) shown in FIG. 22 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Additionally, the layer 50 of insulating material shown in FIG. 10 is formed so that the electrodes 16(2), 18(2), and 20(2) and layer 50 can move and resonate.
The method for making the resonator 10(8) shown in FIG. 23 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the layer 32 of insulating material shown in FIG. 2 is patterned and etched to form two openings which are filled with the conductive material 36 to form the two electrodes 16(1) and 20(1). Additionally, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Further, the layer 50 of insulating material shown in FIG. 10 is formed so that the electrodes 16(2) and 20(2) and layer 50 can move and resonate.
The method for making the resonator 10(9) shown in FIG. 24 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, a substrate made of a layer 17 of conductive material is provided that is not patterned or etched and forms the common electrode 17, although other arrangements can be used, such as using other numbers and types of layers of materials. Additionally, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Further, the layer 50 of insulating material shown in FIG. 10 is formed so that the electrodes 16(2) and 20(2) and layer 50 can move and resonate.
The method for making the resonator 10(10) shown in FIG. 25 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, a substrate made of a layer 17 of conductive material is provided that is not patterned or etched and forms the common electrode 17, although other arrangements can be used, such as using other numbers and types of layers of materials and patterning or etching common electrode 17 in to two or more common electrodes. Additionally, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Further, the layer 50 of insulating material shown in FIG. 10 is formed so that the electrodes 16(2) and 20(2) and layer 50 can move and resonate.
The resonator 10(10) also includes a binding layer 101 and probes 103 so that the resonator 10(10) can function as a sensing system for a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. In this particular embodiment, the binding layer 101, such as gold, is formed on the layer 50, although other types of binding layers can be used which can be attached to other layers. Probes 103, such as a target binding group or a chemical attractant, are attached to the binding layer 101 in a manner well known to those of ordinary skill in the art although a binding layer may not be used if so desired.
The probes 103 may each include (i) one or more binding groups which enable them to be coupled to the binding layer 101 (either directly or via a coupling agent) and (ii) one or more target-binding groups that bind to a target substance. For example, the probes 103 may be attached to the layer 101 of gold deposited and patterned on at least a portion of the surface of the vibrating member using a linker with thio termination. It is well known that the HS− termination will readily bind to the layer 101 of gold.
A number of strategies are available for attaching the probes 103, such as a target binding group or a chemical attractant, to the binding layer 101, depending upon the type of probe which is ultimately to be attached thereto. For example, the available strategies for attaching the probes 103 include, without limitation, covalently bonding a probe to the surface of the binding layer 101, ionically associating the probe with the surface of the binding layer 101, adsorbing the probe onto the surface of the binding layer 101, or the like.
Such association can also include covalently or noncovalently attaching the probe 103 to another moiety (e.g., of a coupling agent), which in turn is attached to the surface of the binding layer 101. In particular, the surface of the binding layer 101 may be first functionalized (i.e., primed) with a coupling agent which is attached to the surface thereof. This is achieved by providing a coupling agent precursor and then binding the coupling agent precursor to the surface of the binding layer 101. Once the surface has been primed, the probe is exposed to the primed surface under conditions effective to (i) covalently or non-covalently bind to the coupling agent or (ii) displace the coupling agent such that the probe binds directly to the binding layer 101.
Where the probes 103 contain two or more target-binding groups, it is possible that the target-binding groups may also interact and bind to the primed surface of the binding layer 101. To preclude this from occurring, the primed binding layer 101 can also be exposed to a blocking agent. The blocking agent essentially minimizes the number of sites where the one or more probes can attach to the surface of the binding layer. Exposure to the blocking agent can be carried out prior to exposing the primed surface of the binding layer to the probes 103 or simultaneously therewith. The blocking agents can be structurally similar to the probes 103 except that they lack a target-binding group or the blocking agents can simply be simple end-capping agents.
The binding of the probes 103 to the binding layer 101 is carried out under conditions which are effective to allow the one or more target-binding groups thereon to remain available for binding to the target substance.
The one or more target-binding groups can include, without limitation, an amino group, a thiol, a hydroxyl, an alkyl chain, an ester, a carboxylic acid, an aromatic, a heterocycle, or a combination thereof.
Suitable probes generally include, without limitation, non-polymeric small molecules, polypeptides, and proteins. Exemplary polypeptides include, without limitation, polypeptides which bind organic warfare agents such as tabun, sarin, soman, GF, VX, mustard agents, botulinium toxin, Staphylococcus entertoxin B, and saitotoxin.
By way of example, the probes 103 may be chemical molecular probes for detecting toxic substances, such as nerve gas or other biological hazardous materials (e.g., naturally occurring toxins or organic warfare agents, etc.). These target substances can be detected from any source, including food samples, water samples, homogenized tissue from organisms, etc. Moreover, the sensing system of the present invention can also be used effectively to detect multiple layers of biomolecular interactions, termed “cascade sensing.” Thus, a target, once bound, becomes a probe for a secondary target.
The probes 103 have extremely high specificity and will detect a predetermined target substance. The probability of a false positive is very small.
The method for making the resonator 10(11) shown in FIG. 27 is the same as the method described above for making the resonator 10(1) shown in FIGS. 11, 13, and 16 except as described below. In this particular embodiment, the deposited layer of conductive material 36 is not patterned or etched and forms the common electrode 17, although other arrangements can be used, such as starting with a substrate made of a conductive material which acts as the common electrode 17. Additionally, the layer 38 of insulating material shown in FIG. 3 is not patterned or etched and the layer 22(1) of the member 14(1) is formed on the layer 38. Further, the layer 50 of insulating material shown in FIG. 10 is formed so that the electrodes 16(2) and 20(2) and layer 50 can move and resonate.
An input voltage system 105 is coupled to the input 52 to provide an input voltage signal at a resonant frequency, although other types of input systems which provide other types of input signals can be used. An output voltage monitoring system 107 is coupled to the output 54 to monitor the frequency of the output voltage signal, to compare the current reading of the output voltage signal against prior readings or a stored baseline reading to detect a change in the output voltage signal, and provides a notification on a display or other manner when a change in the output signal is detected. By way of example only, the output voltage monitoring system 107 may detect a change in the frequency or a frequency shift in the output voltage signal. Although an output voltage monitoring system 107 is shown, other types of output systems, such as an output processing system with a processor and memory with programmed instructions to carry out the methods described herein, could be used.
The resonator 10(11) also includes a binding layer 101 and probes 103 so that the resonator 10(11) can function as a biohazard or insect or pest or bacteria or fungus, etc. sensing system. In this particular embodiment, the binding layer 101, such as gold, is formed on the layer 50, although other types of binding layers can be used which can be attached to other layers. Probes 103 are attached to the binding layer 101 in a manner well known to those of ordinary skill in the art, and as described above. For example, the probes 103 may be attached to the layer 101 of gold deposited and patterned on at least a portion of the surface of the vibrating member using a linker with thio termination. It is well known that the HS− termination will readily bind to the layer 101 of gold. By way of example, the probes 103 may be chemical molecular probes for detecting toxic substances, such as nerve gas or other biological hazardous materials. Detailed descriptions and examples of suitable probes, attachment strategies, etc. for this embodiment of the present invention are described above with respect to resonator 10(10). The probes 103 have extremely high specificity and will detect a predetermined target substance. The probability of a false positive is very small.
An input frequency system 109 is coupled to the input 52 to provide an input signal at a resonant frequency, although other types of input systems which provide other types of input signals can be used. A frequency analyzer 111 is coupled to the output 54 to monitor the frequency of the output signal, to compare the current reading of the output signal against prior readings or a stored baseline reading to detect a change in the output voltage signal, and provides a notification on a display or other manner when a change in the output signal is detected. By way of example only, the frequency analyzer 111 may detect a change in the frequency or a frequency shift in the output signal. Although a frequency analyzer 111 is shown, other types of output systems, such as an output processing system with a processor and memory with programmed instructions to carry out the methods described herein, could be used.
The method for making the resonator 10(12) shown in FIG. 29 is the same as the method described above for making the resonator 10(10) shown in FIG. 25 except as described below. The resonator 10(12) also includes probes 103, such as a target binding group or a chemical attractant, so that the resonator 10(12) can function as a sensing system for a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. The probes 103, in this example an attractant, is attached directly to the electrodes 16(2) and 18(2) in a manner well known to those of ordinary skill in the art, although as described earlier a binding layer 101 could be used. A variety of different types of probes 103 could be used, such as pheromones. By way of example only, insects are induced to reside on and/or to deposit material, such as eggs or fecal matter, by the attractant used as the probe 103. The additional mass by the target's presence or deposit on the probe 103 on electrode 16(2) results in a change in resonant frequency which in turn indicates the presence of the target insect or other species.
A number of strategies are available for attaching probes 103 to the electrodes 16(2) and 20(2) depending upon the type of attractant which is ultimately to be attached thereto. Additionally, a binding layer 101 could be used to attach the attractant used for probe 103. The available strategies for attaching the probes 103 include, without limitation, covalently bonding probes 103 to the electrodes 16(2) and 20(2), ionically associating the probes 103 with the surface of the electrodes 16(2) and 20(2), adsorbing the probes 103 onto the surface of the electrodes 16(2) and 20(2), or the like.
Although only resonators 10(10)-10(12) are shown with a binding layer 101 with probes 103 to form sensing systems for a target such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, the other resonators 10(1)-10(9) can also be modified in the same manner to form sensing systems for a target such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. In resonators where the member moves and resonates, the chamber 12 will have an opening to the environment being tested.
The operation of the resonators 10(1)-10(12) to pass one or more signals will now be discussed with reference to FIGS. 13-25, 27, and 29. If a tuning bias is applied across the tuning bias inputs 51(2) and 51(2), the resonant frequency of the resonator can be tuned to a particular frequency. When an input signal that includes the resonant frequency is received on input lead 52 or across input leads 52(1) and 52(2), the incoming signal applies or induces a varying signal, such as a varying amplitude signal or a varying frequency signal, on the pairs of input electrodes 16(1), 16(2), 17, 18(1), and/or 18(2) coupled to the input lead 52 or across the leads 52(1) and 52(2) which correlates to the resonant portion of the incoming signal. The varying signal on the pairs of input electrodes 16(1)-16(2) and 18(1)-18(2) or 16(1)-16(2) causes the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or 16(2) and 18(2) to oscillate in response to the received varying signal. The movement of the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or 16(2) and 18(2) causes a varying signal, such as a varying amplitude signal or a varying frequency signal, to be applied or induced on the pairs of output electrodes 20(1)-20(2) or output electrode 20(2) and common electrode 17 which is transmitted out as an output signal on the output lead 54 or across the output leads 54(1) and 54(2).
When the output lead 54 or leads 54(1) and 54(2) receives a signal at the resonant frequency to transmit, the signal induces a varying signal, such as a varying amplitude signal or a varying frequency signal, on the pairs of output electrodes 20(1)-20(2) or output electrode 20(2) in response to the signal. The varying signal on the pairs of output electrodes 20(1)-20(2) or output electrode 20(2) causes the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or 16(2) and 18(2) to oscillate in response to the varying signal. The movement of the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or electrodes 16(2) and 18(2) causes a varying signal, such as a varying amplitude signal or a varying frequency signal, to be applied or induced on the pairs of input electrodes 16(1)-16(2) and 18(1)-18(2), electrodes 16(1)-16(2), electrodes 16(2) and 18(2) and 17, or electrodes 16(2) and 17 which is transmitted out as an outgoing signal via the input leads 52 or across the input leads 52(1) and 52(2). Accordingly, these resonators 10(1)-10(11) can be used to eliminate the need for some components, such as separate transmit/receive cell phone boards.
By way of example only, an embedded charge density of 1e12 charges per cm2 on the member and an oscillation sufficient to change the induced charge on an output set of electrodes by 1% will cause a voltage swing of 0.36V. A 1% change in induced charge for a 6e12 charges per cm2 case yields an output swing of 2.16V. Thus an average oscillation displacement of only a few tens of angstroms yields a significant output signal. These calculations are based on a percentage change in the magnitude of the induced charge on the output capacitor based on the relative position of the embedded charge 15 residing in the member 14(1) or 14(2).
The operation of the resonators 10(10)-10(12) as a sensing systems for a target such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, will now be discussed with reference to FIGS. 25-29. As described earlier, when an incoming signal that includes the resonant frequency is received on input lead 52 or across input leads 52(1) and 52(2), the incoming signal applies or induces a varying signal, such as a varying amplitude signal or a varying frequency signal, on the pairs of input electrodes 16(1) and 16(2) and 18(1) and 18(2), electrodes 16(2), 17, and 18(2), electrodes 16(1) and 16(2), or electrodes 16(2) and 17 coupled to the input lead 52 or across the leads 52(1) and 52(2) which correlates to the resonant portion of the incoming signal. The varying signal on the pairs of input electrodes 16(1)-16(2) and 18(1)-18(2) or 16(1)-16(2) causes the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or electrodes 16(2) and 18(2) to oscillate in response to the received varying signal. The movement of the member 14(1) or 14(2) with the embedded charge 15 or the electrodes 16(2) or electrodes 16(2) and 18(2) causes a varying signal, such as a varying amplitude signal or a varying frequency signal, to be applied or induced on the pairs of output electrodes 20(1)-20(2) or output electrode 20(2) and common electrode 17 which is transmitted out as an output signal on the output lead 54 or across the output leads 54(1) and 54(2). As long as the input signal does not change, the output signal from the resonators 10(10) and 10(11) should remain substantially constant.
As described earlier, the resonators 10(10)-10(12) include probes 103 which are bound to a layer 101 or directly on a vibrating portion of the resonators 10(10)-10(12). These probes 103 are designed to bind with a particular type or types of thing, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion. When the resonators 10(10)-10(12) are being used as sensing systems for a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, the resonators 10(10)-10(12) are placed in an environment where the target may be found. If the target is not present, then the output signal on output 54 or outputs 54(1) and 54(2) from the resonators 10(10)-10(12) should remain substantially constant.
If the target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion, is present in the environment being examined, then depending on the type of probe 103, the target may bind to, reside on, or leave a deposit, such as fecal matter or eggs, on the probes 103. This binding of target substance to or deposit on the probes 103 which will increase the mass of the vibrating member and thereby lower the resonant frequency of the resonators 10(10)-10(12). If the input signal on input 52 or 52(1) and 52(2) remains at the initial resonant frequency before the probes 103 were exposed to the target substance, then the output signal on output 54 or outputs 54(1) and 54(2) from the resonators 10(10)-10(12) will decrease as target binding occurs. Referring to FIG. 26, the initial and post target binding output signal vs. driving frequency is illustrated. As the actual resonant frequency decreases as a result of target binding, the output signal on output 54 or 54(1) and 54(2) decreases indicating that the target substance has been detected
Alternatively, the resonant frequency can be monitored. A positive binding event or deposit on the probes 103 will cause the resonant frequency to decrease and the output will be a measure of a shift in resonant frequency. Referring to FIG. 28, a frequency output shift as a measure of binding of a target substance on probes 103 is illustrated.
Accordingly, the present invention also provides an effective, simple and inexpensive sensing system with a resonator for a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion.
Another advantage of the present invention is that the resonator has embodiments where the embedded charge layer is totally isolated and sealed from the ambient environment. As a result, no contamination can damage, harm or otherwise interfere with the intended function of detection of a target, such as a hazardous substance, gas, insect, bacteria, fungus, spore, virus, or prion.
Having thus described the basic concept of the invention, it will be rather apparent to those skilled in the art that the foregoing detailed disclosure is intended to be presented by way of example only, and is not limiting. Various alterations, improvements, and modifications will occur and are intended to those skilled in the art, though not expressly stated herein. These alterations, improvements, and modifications are intended to be suggested hereby, and are within the spirit and scope of the invention. Additionally, the recited order of processing elements or sequences, or the use of numbers, letters, or other designations therefor, is not intended to limit the claimed processes to any order except as may be specified in the claims. Accordingly, the invention is limited only by the following claims and equivalents thereto.

## Claims

1. A sensing system for at least one target, the system comprising:
a member with an embedded charge; 
at least one input electrode; 
at least one output electrode; 
at least one common electrode, the at least one input and output electrodes are spaced from and on substantially opposing sides of the member from the at least one common electrode, wherein at least one of the member and the input and output electrodes is movable with respect to the other; 
one or more probes that engage with the target, the probes are connected to the at least one of the member and the input and output electrodes which is movable with respect to the other; 
an input system coupled between the at least one input electrode and the at least one output electrode, the input system providing an input signal; and 
an output monitoring system coupled between the at least one output electrode and the at least one common electrode, the output monitoring system detecting a change in an output signal when the target engages with the one or more probes.

2. The system as set forth in claim 1 wherein the at least one common electrode comprises a corresponding input electrode for each of the at least one input electrode and a corresponding output electrode for each of the at least one output electrode.

3. The system as set forth in claim 2 wherein the at least one output electrode and the corresponding output electrode have a substantially circular shape and the at least one input electrode and the corresponding input electrode are arranged in a substantially annulus fashion around and spaced from the at least one output electrode and the corresponding output electrode.

4. The system as set forth in claim 2 wherein the at least one input electrode and the corresponding input electrode have a substantially circular shape and the at least one output electrode and the corresponding output electrode are arranged in a substantially annulus fashion around and spaced from the at least one input electrode and the corresponding input electrode.

5. The system as set forth in claim 2 further comprising at least two of the input electrodes, each of the at least two input electrodes having one of the corresponding input electrodes spaced from and on a substantially opposing side of the member, an input lead coupled to one of the two input electrodes and the one of the corresponding input electrodes for the one of the two input electrode, a tuning bias input coupled to the other of the two input electrodes and the other one of the corresponding input electrodes for the other one of the two input electrode, and an output lead coupled to the at least one output electrode and the corresponding output electrode.

6. The system as set forth in claim 2 further comprising at least two of the input electrodes, each of the at least two input electrodes having one of the corresponding input electrodes spaced from and on a substantially opposing side of the member, input leads coupled to each of the at least two input electrodes, an output lead coupled to the at least one output electrode, and a common reference lead coupled to the corresponding input electrodes and the corresponding output electrode.

7. The system as set forth in claim 1 wherein the at least one common electrode comprises a substrate comprising a conductive material.

8. The system as set forth in claim 1 wherein the member is movable with respect to the input and output electrodes.

9. The system as set forth in claim 1 wherein the input and output electrodes are movable with respect to the member.

10. The system as set forth in claim 1 further comprising a chamber, wherein at least one portion of the member is connected to the chamber and the member extends at least partially across the chamber.

11. The system as set forth in claim 10 wherein the member is connected on at least substantially opposing sides of the chamber.

12. The system as set forth in claim 10 wherein the member is fixed.

13. The system as set forth in claim 10 wherein the member has at least one elongated side and at least one end, wherein the member is connected to the chamber along the at least one elongated side.

14. The system as set forth in claim 10 wherein the member has at least one elongated side and at least one end, wherein the member is connected to the chamber at the at least one end.

15. The system as set forth in claim 10 wherein the member has at least one pair of opposing surfaces, wherein at least one of the opposing surfaces is fixed with respect to the chamber.

16. The system as set forth in claim 1 wherein the member is at least one of a single dielectric layer, a composite of two or more dielectric layers, a floating conductor, and an electret.

17. The system as set forth in claim 1 wherein the member is made from one or more materials selected from a group consisting of silicon oxide, silicon dioxide, silicon nitride, aluminum oxide, tantalum oxide, tantalum pentoxide, titanium oxide, titanium dioxide, barium strontium titanium oxide.

18. The system as set forth in claim 1 further comprising an input lead coupled to the at least one input electrode, an output lead coupled to the at least one output electrode, and a common reference coupled to the common electrode.

19. A method for making a resonator, the method comprising:
providing a member with an embedded charge; 
providing at least one input electrode; 
providing at least one output electrode; 
providing at least one common electrode, the at least one input and output electrodes are spaced from and on substantially opposing sides of the member from the at least one common electrode, wherein at least one of the member and the input and output electrodes is movable with respect to the other; 
providing one or more probes that engage with the target object, the probes are connected to the at least one of the member and the input and output electrodes which is movable with respect to the other; 
coupling an input system between the at least one input electrode and the at least one output electrode, the input system providing an input signal; and 
coupling an output monitoring system between the at least one output electrode and the at least one common electrode, the output monitoring system detecting a change in an output signal when the target binds with the one or more probes.

20. The method as set forth in claim 19 wherein the providing at least one common electrode further comprises providing a corresponding input electrode for each of the at least one input electrode and a corresponding output electrode for each of the at least one output electrode.

21. The method as set forth in claim 19 wherein the member is movable with respect to the input and output electrodes.

22. The resonator as set forth in claim 19 wherein the input and output electrodes are movable with respect to the member.

23. The method as set forth in claim 19 further comprising providing a chamber, wherein at least one portion of the member is connected to the chamber and the member extends at least partially across the chamber.

24. The method as set forth in claim 23 wherein the member is connected on at least substantially opposing sides of the chamber.

25. The method as set forth in claim 23 wherein the member is fixed.

26. The method as set forth in claim 23 wherein the member has at least one elongated side and at least one end, wherein the member is connected to the chamber along the at least one elongated side.

27. The method as set forth in claim 23 wherein the member has at least one elongated side and at least one end, wherein the member is connected to the chamber at the at least one end.

28. The method as set forth in claim 23 wherein the member has at least one pair of opposing surfaces, wherein at least one of the opposing surfaces is fixed with respect to the chamber.

29. The method as set forth in claim 23 wherein the providing a chamber further comprises:
providing a first insulating layer; 
providing a second insulating layer over at least a portion of the first insulating layer; 
etching a first trench in second insulating layer which extends to the first insulating layer; 
filling at least a portion of the first trench with a first sacrificial material, wherein the providing a member is on at least a portion of the first layer of sacrificial material and on at least a portion of the second insulating layer; 
depositing a third insulating layer over at least a portion of the member; 
etching a second trench in third insulating layer which extends to the member; 
filling at least a portion of the second trench with a second layer of sacrificial material; 
depositing a fourth insulating layer on at least a portion of the third insulating layer; and 
removing the first and second layers of sacrificial material.

30. The method as set forth in claim 29 wherein the providing a member further comprises depositing the member on at least a portion of the first layer of sacrificial material and on at least a portion of the second insulating layer.

31. The method as set forth in claim 23 wherein the providing a chamber further comprises:
providing a first insulating layer; 
providing a second insulating layer over at least a portion of the first insulating layer, wherein the providing a member is on at least a portion of the second insulating layer; 
depositing a third insulating layer over at least a portion of the member; 
etching a second trench in third insulating layer which extends to the member; 
filling at least a portion of the second trench with a first layer of sacrificial material; 
depositing a fourth insulating layer on at least a portion of the third insulating layer; and 
removing the first layer of sacrificial material.

32. The method as set forth in claim 31 wherein the providing a member further comprises depositing the member on at least a portion of the second insulating layer.

33. The method as set forth in claim 19 wherein the member is at least one of a single dielectric layer, a composite of two or more dielectric layers, a floating conductor, and an electret.

34. The method as set forth in claim 19 wherein the member is made from one or more materials selected from a group consisting of silicon oxide, silicon dioxide, silicon nitride, aluminum oxide, tantalum oxide, tantalum pentoxide, titanium oxide, titanium dioxide, barium strontium titanium oxide.

35. The method as set forth in claim 19 wherein the at least one output electrode and the corresponding output electrode have a substantially circular shape and the at least one input electrode and the corresponding input electrode are arranged in a substantially annulus fashion around and spaced from the at least one output electrode and the corresponding output electrode.

36. The method as set forth in claim 19 wherein the at least one input electrode and the corresponding input electrode have a substantially circular shape and the at least one output electrode and the corresponding output electrode are arranged in a substantially annulus fashion around and spaced from the at least one input electrode and the corresponding input electrode.

37. The method as set forth in claim 19 wherein the providing a member further comprises storing the embedded charge in the member.

38. The method as set forth in claim 37 wherein the storing the embedded charge in the member comprises at least one of injecting electrons into the member, using ballistic injection to embed the charge, using polarization to embed the charge, and using high field injection to embed the charge.

39. The method as set forth in claim 19 wherein the providing at least one common electrode further comprises providing a substrate comprising at least one conductive material, the substrate providing the at least one common electrode.

40. The method as set forth in claim 19 wherein the providing at least one common electrode further comprises providing a first conducting layer to provide the at least one common electrode.

41. The method as set forth in claim 40 wherein the providing at least one common electrode further comprises etching the first conducting layer to form a corresponding input electrode for each of the at least one input electrode and to form a corresponding output electrode for each of the at least one output electrode.

42. The method as set forth in claim 40 wherein the providing the at least one input electrode and providing the at least one output electrode further comprises:
depositing a second conducting layer on at least a portion of the second layer of sacrificial material to provide the other one of the electrodes for at least one of the pairs of electrodes; and 
etching the second conducting layer to form the at least one input electrode and the at least one output electrode.

43. The method as set forth in claim 42 wherein the etching of the first conducting layer and the second conducting layer for the at least one output electrode and the corresponding output electrode each have a substantially circular shape and for the at least one input electrode and the corresponding input electrode a substantially annulus shape around the at least one output electrode and the corresponding output electrode with the substantially circular shape.

44. The method as set forth in claim 42 wherein the etching of the first conducting layer and the second conducting layer for the at least one input electrode and the corresponding input electrode each have a substantially circular shape and for the at least one output electrode and the corresponding output electrode a substantially annulus shape around the at least one input electrode and the corresponding input electrode with the substantially circular shape.

45. A method for detecting a target, the method comprising:
receiving an input signal around a resonant frequency; 
applying a first varying signal on at least a first pair electrodes in response to the received input signal, each of the first pair of electrodes spaced from and on substantially opposing sides of a member with an embedded charge; 
oscillating one of the member and one of the first pair of electrodes in response to the applied varying signal on the first pair of electrodes; 
transmitting an output signal based on a second varying signal which is induced on the second pair of electrodes in response to the oscillation of the member; 
placing probes that engage with the target and are located on one of the member and one of the first pair of electrodes in an environment to be monitored; and 
signaling a presence of the target when a change in the output signal is detected.

46. The method as set forth in claim 45 wherein the first varying signal comprises at least one of an amplitude modulated input signal and a frequency modulated input signal.

47. The method as set forth in claim 45 wherein the at least a first pair of electrodes and the at least a second pair of electrodes share a common electrode.

48. The method as set forth in claim 45 wherein the at least a first pair of electrodes and the at least a second pair of electrodes are separate sets of electrodes.

49. The method as set forth in claim 45 wherein the at least a first pair of input electrodes comprises at least two of the first pair of input electrodes.

50. The method as set forth in claim 49 further comprising applying a tuning bias to one of the at least two of the first pair of input electrodes.