# Patent

## Title
Method for confirming the exposure on chrysene

## Abstract

The present invention relates to a method for confirming the exposure on chrysene using a DNA fragment whose expression is increased or decreased specifically when it is exposed to chrysene. The method of the present invention is effective in determination of risk by chrysene and monitoring the chrysene exposure, so that it can be effectively used as a tool for examining the mechanism of chrysene induced toxicity.

## Background
TECHNICAL FIELD
The present invention relates to a method for confirming the exposure on chrysene by using a marker gene up-regulated or down-regulated by chrysene.
BACKGROUND ART
Chrysene, one of polycyclic aromatic hydrocarbons, is discharged in the environment because of incomplete combustion. It has been used for organic material synthesis or chemical research, but has not been produced as a commercial product. In the environment, the movement of chrysene is hardly detected in soil because it does not have volatility and it is absorbed onto sediment and suspended solids in water, indicating it does not have volatility on the surface of water, either. Decomposition of a microorganism is slower in water than in soil. Absorption in the soil makes the decomposition of a microorganism even slower. Hydrolysis of it does not occur in the environment and the level of its concentration in aquatic living organisms is very low. Chrysene exists as particles in the air and thus it can be eliminated by physical methods.
According to a report by the Ministry of Environment Korea (1997) investigating chrysene concentration in the domestic air and underground shopping centers, the chrysene concentration was 1.80 ng/m3 in Seoul residential area and 1.2 ng/m3 in Taepyeong-dong, Daejeon, Korea. To investigate food contamination by polycyclic aromatic hydrocarbons, the chrysene concentrations in meat processed products sold in 9 cities in Korea were examined. As a result, the concentration of chrysene was 0.176 μg/kg in smoked products, 0.842 μg/kg in poultry, 0.143 μg/kg in meats (Kim M C et al., Korea Food & Drug Administration, 2001 Endocrine Discruptors Research report, 2001, and 0.82 μg/kg in edible oil and fat (Lee J O et al., Korea Food & Drug Administration, Research report 6, 790. 2002).
Chrysene was absorbed via oral pathway in animal tests. It was also absorbed through skin but the absorption rate was very slow and most of the absorbed chrysene was discharged through feces [ASTDR (Agency for Toxic Substance and Disease Registry), 1990). Chrysene is converted into dihydrodiols by an enzyme and further changed into diol-epoxide capable of binding to DNA to produce DNA byproduct. It was reported that diol-epoxide derivatives of chrysene could be potential carcinogens or mutagens. In Ames tests using salmonella TA110 and TA98 strains, chrysene showed mutagenicity in rats even under normal metabolic activity of the liver [HSDB (Hazardous Substance Data bank)].
Reports on the carcinogenicity of the polycyclic aromatic hydrocarbons and changes of gene expression induced by the same have been made intermittently but those reports are mostly limited to benzo[a]pyrene, the most representative polycyclic aromatic hydrocarbon. Despite chrysene has high potential for causing cancer in human, there are not enough data to evaluate risk in human and the screening method is still limited to the conventional GC-MS (Gas Chromatography-Mass Spectrometer) and HPLC (High Performance Liquid Chromatography). GC-MS or HPLC enables quantification but requires high cost equipments and set up for proper conditions for analysis. Therefore, a faster and easier screening method such as real-time PCR (real-time reverse transcript polymerase chain reaction) with primers or DNA microarray chip is required to establish a molecular index to detect toxicity and to evaluate risk in human. It is also important to establish a countermeasure for the exposure on chrysene and a regulation method of the same by using the above method.
Genome sequencing project of various species including 6 species of mammals and 292 species of microorganisms has been completed and reported to NCBI (National Center for Biotechnology Information). Based on this huge data, genome-wide expression study has been undergoing to disclose functions of those genes. DNA microarray is also performed to analyze expressions of thousands of genes at a time (Schena M et al., Proc. Natl. Acad. Sci. USA 93: 10614-10619, 1996).
Microarray is prepared by integrating cDNA (complementary DNA) or 20-25 base pair long oligonucleotide sets on a glass substrate. cDNA microarray has been produced in laboratories and companies such as Agilent and Genomic Solutions, in which cDNAs are fixed by a mechanical method or ink jetting on a chip (Sellheyer K and Belbin T J, J. Am. Acad. Dermatol. 51: 681-692, 2004). Oligonucleotide microarray has been produced in Affymetrix by direct synthesis on a chip via photolithography and in Agilent by fixing synthetic oligonucleotides on a chip (Sellheyer K et al., Am. Acad. Dermatol. 51: 681-692, 2004).
To analyze gene expression, RNAs are extracted from samples such as tissues, which are hybridized with oligonucleotide on DNA microarray. The obtained RNA is labeled with a fluorescent material or an isotope and converted into cDNA. In oligo-microarray, the control and the experimental groups are labeled with two different fluorescent materials (for example, Cy3 and Cy5), followed by hybridization simultaneously on the chip. Images are optically scanned to measure the strength of fluorescence and the results are analyzed. By comparing the strengths of the two different fluorescent materials, gene expression is determined (Somasundaram K et al., Genomics Proteomics I: 1-10, 2002).
The recent DNA microarray based high tech toxicogenomics enables the analysis and quantification of gene expression patterns in specific tissues or cell lines induced by a medicant or novel a drug candidate and other chemicals. That is, by analyzing the expression frequency of a specific gene in a specific cell, it is possible to identify a gene causing side effects of such drugs or harmful action of such environmental pollutant materials. Then, it is further possible to understand the molecular mechanism related to such side effects and functions of drugs and environmental pollutant materials.
The present inventors screened genes up-regulated or down-regulated by chrysene by observing and analyzing gene expression profiles of chrysene in HepG2, a human Hepatoma cell line, using oligo-microarray on which 41,000 human genes are integrated, and further completed this invention by confirming the genes capable of confirming the exposure on chrysene by investigating expressions of target genes by real-time RT-PCR and by establishing a method for confirming the exposure on chrysene.
DISCLOSURE
Technical Problem
It is an objective of the present invention to provide a method for confirming the exposure on chrysene by using a marker gene up-regulated or down-regulated by chrysene.
Technical Solution
To achieve the above objective, the present invention provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group; and,
2) determining the exposure on chrysene by comparing the expression of the gene whose expression is affected by chrysene with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
The present invention also provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group;
2) synthesizing cDNAs with the RNAs separated from the experimental group and the control of step 1) and labeling them with different fluorescent materials;
3) hybridizing the cDNAs of step 2) each labeled with different fluorescent materials with DNA microarray chip comprising oligonucleotide containing the full length or a part of a gene whose expression is changed by chrysene or its complement oligonucleotide;
4) analyzing the DNA microarray chip reacted in step 3); and
5) determining the exposure on chrysene by comparing the expression of the gene whose expression is affected by chrysene with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
In addition, the present invention provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group;
2) performing real-time RT-PCR (reverse transcript polymerase chain reaction) with the RNAs of step 1) using a primer set capable of amplifying the gene whose expression changed by chrysene; and
3) determining the exposure on chrysene by comparing the expression of the gene of step 2) with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
Advantageous Effect
The method of the present invention using a DNA fragment whose expression is increased or decreased specifically when it is exposed to chrysene is effective in determination of risk by chrysene and monitoring the chrysene exposure, so that it can be effectively used as a tool for examining the mechanism of chrysene induced toxicity.



DESCRIPTION OF DRAWINGS
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
The application of the preferred embodiments of the present invention is best understood with reference to the accompanying drawings, wherein:
FIG. 1 is a graph illustrating the cytotoxicity of chrysene in a human Hepatoma cell line.
FIG. 2 is a diagram illustrating the gene expression pattern in the human Hepatoma cell line treated with chrysene, investigated by using the microarray chip.



MODE FOR INVENTION
Hereinafter, the present invention is described in detail.
The present invention provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group; and,
2) determining the exposure on chrysene by comparing the expression of the gene whose expression is affected by chrysene with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
The somatic cells of step 1) are the cells originated from human liver or liver cancer cells and tissues and preferably HepG2 cells.
The comparing the expression of a gene in step 2) is performed at the level of mRNA or protein. At this time, the mRNA level comsetson can be performed by oligonucleotide or polynucleotide microarray or RT-PCR. And, the protein level comsetson can be performed by protein microarray or ELISA.
The present invention also provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group;
2) synthesizing cDNAs with the RNAs separated from the experimental group and the control of step 1) and labeling them with different fluorescent materials;
3) hybridizing the cDNAs of step 2) each labeled with different fluorescent materials with DNA microarray chip comprising oligonucleotide containing the full length or a part of a gene whose expression is changed by chrysene or its complement oligonucleotide;
4) analyzing the DNA microarray chip reacted in step 3); and
5) determining the exposure on chrysene by comparing the expression of the gene whose expression is affected by chrysene with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
The somatic cells of step 1) are the cells originated from human liver or liver cancer cells and tissues and preferably HepG2 cells.
In the above method, the DNA microarray of step 4) is the whole human genome oligo microarray (Agilent, USA), but not always limited thereto, and any microarray that is loaded with those genes supposed to be up-regulated or down-regulated (see Table 2 and Table 3), among human genomes, can be used. In a preferred embodiment of the present invention, the DNA microarray chip prepared by the present inventors was used. And for the analysis of step 4), GenePix 4.1 software (Axon Instruments, USA) is preferably used, but not always limited thereto, and any software for analysis known to those in the art can be used.
The above oligonucleotide or its complement oligonucleotide contains 18-30 nucleic acids of the gene whose expression is changed by chrysene and more preferably contains 20-25 nucleic acids.
In this invention, the DNA microarray chip can be prepared by the method well known to those in the art. The method for preparing the microarray chip is as follows. The gene whose expression is affected by chrysene used as a probe DNA molecule is fixed on the substrate of DNA chip. For the fixation, the micropipetting method using piezoelectric way or the method using pin type spotter is used, but not always limited thereto. The substrate of the DNA microarray chip is preferably coated with an active group selected from the group consisting of amino-silane, poly-L-lysine and aldehyde, but not always limited thereto. The substrate can be selected from the group consisting of slide glass, plastic, metal, silicon, nylon membrane and nitrocellulose membrane, but not always limited thereto.
In the above method, the DNA microarray of step 4) is the whole human genome oligo microarray (Agilent, USA), but not always limited thereto, and any microarray that is loaded with those genes supposed to be up-regulated or down-regulated (see Table 2 and Table 3), among human genomes, can be used. And for the analysis of step 4), GenePix 4.1 software (Axon Instruments, USA) is preferably used, but not always limited thereto, and any software for analysis known to those in the art can be used.
In addition, the present invention provides a method for confirming the exposure on chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample, the experimental group, and from somatic cells of the control group;
2) performing real-time RT-PCR (reverse transcript polymerase chain reaction) with the RNAs of step 1) using a primer set capable of amplifying the gene whose expression changed by chrysene; and
3) determining the exposure on chrysene by comparing the expression of the gene of step 2) with that of the control, when the expression of the gene is significantly changed, compared with that of the control, the gene is regarded as being exposed to chrysene.
In the method of the present invention, the primer set of step 2) can include any sense or antisense primer set that is capable of amplifying a gene whose expression is changed by chrysene and is designed to produce the amplified product of 100-300 bp in size. The primer of the present invention is preferably selected from the group consisting of the following primer sets 1-35, but not always limited thereto:
primer sets 1—Sense primer represented by SEQ. ID. NO: 1 and anti-sense primer represented by SEQ. ID. NO: 2;
primer sets 2—Sense primer represented by SEQ. ID. NO: 3 and anti-sense primer represented by SEQ. ID. NO: 4;
primer sets 3—Sense primer represented by SEQ. ID. NO: 5 and anti-sense primer represented by SEQ. ID. NO: 6;
primer sets 4—Sense primer represented by SEQ. ID. NO: 7 and anti-sense primer represented by SEQ. ID. NO: 8;
primer sets 5—Sense primer represented by SEQ. ID. NO: 9 and anti-sense primer represented by SEQ. ID. NO: 10;
primer sets 6—Sense primer represented by SEQ. ID. NO: 11 and anti-sense primer represented by SEQ. ID. NO: 12;
primer sets 7—Sense primer represented by SEQ. ID. NO: 13 and anti-sense primer represented by SEQ. ID. NO: 14;
primer sets 8—Sense primer represented by SEQ. ID. NO: 15 and anti-sense primer represented by SEQ. ID. NO: 16;
primer sets 9—Sense primer represented by SEQ. ID. NO: 17 and anti-sense primer represented by SEQ. ID. NO: 18;
primer sets 10—Sense primer represented by SEQ. ID. NO: 19 and anti-sense primer represented by SEQ. ID. NO: 20;
primer sets 11—Sense primer represented by SEQ. ID. NO: 21 and anti-sense primer represented by SEQ. ID. NO: 22;
primer sets 12—Sense primer represented by SEQ. ID. NO: 23 and anti-sense primer represented by SEQ. ID. NO: 24;
primer sets 13—Sense primer represented by SEQ. ID. NO: 25 and anti-sense primer represented by SEQ. ID. NO: 26;
primer sets 14—Sense primer represented by SEQ. ID. NO: 27 and anti-sense primer represented by SEQ. ID. NO: 28;
primer sets 15—Sense primer represented by SEQ. ID. NO: 29 and anti-sense primer represented by SEQ. ID. NO: 30;
primer sets 16—Sense primer represented by SEQ. ID. NO: 31 and anti-sense primer represented by SEQ. ID. NO: 32;
primer sets 17—Sense primer represented by SEQ. ID. NO: 33 and anti-sense primer represented by SEQ. ID. NO: 34;
primer sets 18—Sense primer represented by SEQ. ID. NO: 35 and anti-sense primer represented by SEQ. ID. NO: 36;
primer sets 19—Sense primer represented by SEQ. ID. NO: 37 and anti-sense primer represented by SEQ. ID. NO: 38;
primer sets 20—Sense primer represented by SEQ. ID. NO: 39 and anti-sense primer represented by SEQ. ID. NO: 40;
primer sets 21—Sense primer represented by SEQ. ID. NO: 41 and anti-sense primer represented by SEQ. ID. NO: 42;
primer sets 22—Sense primer represented by SEQ. ID. NO: 43 and anti-sense primer represented by SEQ. ID. NO: 44;
primer sets 23—Sense primer represented by SEQ. ID. NO: 45 and anti-sense primer represented by SEQ. ID. NO: 46;
primer sets 24—Sense primer represented by SEQ. ID. NO: 47 and anti-sense primer represented by SEQ. ID. NO: 48;
primer sets 25—Sense primer represented by SEQ. ID. NO: 49 and anti-sense primer represented by SEQ. ID. NO: 50;
primer sets 26—Sense primer represented by SEQ. ID. NO: 51 and anti-sense primer represented by SEQ. ID. NO: 52;
primer sets 27—Sense primer represented by SEQ. ID. NO: 53 and anti-sense primer represented by SEQ. ID. NO: 54;
primer sets 28—Sense primer represented by SEQ. ID. NO: 55 and anti-sense primer represented by SEQ. ID. NO: 56;
primer sets 29—Sense primer represented by SEQ. ID. NO: 57 and anti-sense primer represented by SEQ. ID. NO: 58;
primer sets 30—Sense primer represented by SEQ. ID. NO: 59 and anti-sense primer represented by SEQ. ID. NO: 60;
primer sets 31—Sense primer represented by SEQ. ID. NO: 61 and anti-sense primer represented by SEQ. ID. NO: 62;
primer sets 32—Sense primer represented by SEQ. ID. NO: 63 and anti-sense primer represented by SEQ. ID. NO: 64;
primer sets 33—Sense primer represented by SEQ. ID. NO: 65 and anti-sense primer represented by SEQ. ID. NO: 66;
primer sets 34—Sense primer represented by SEQ. ID. NO: 67 and anti-sense primer represented by SEQ. ID. NO: 68; and,
primer sets 35—Sense primer represented by SEQ. ID. NO: 69 and anti-sense primer represented by SEQ. ID. NO: 70.
In the method to confirm the exposure of chrysene, the somatic cells are preferably the cells originated from the human liver, or liver cancer cells and tissues, and more preferably HepG2 cells, but not always limited thereto. The sample herein is the one who is suspicious to be exposed to chrysene, and the control is a healthy normal human.
The genes exhibiting 2.0 fold higher or less expressions by chrysene exposure were classified according to the functions. The genes are composed of those genes involved in MAPKinase Signaling Pathway, MAPKKK cascade, p53 Signaling Pathway, apoptosis, response to DNA damage stimulus, Response to stress, immune response, cell cycle, Mitotic cell cycle, Cell differentiation, Cell growth, cell proliferation, Alcohol metabolism, DNA metabolism, lipid metabolism, Amino acid metabolism, metal binding, and Transport.
The gene whose expression is changed by chrysene is characteristically selected from the group consisting of the following genes:
Genebank Accession Number: NM—003954 (MAP3K14, Mitogen-activated protein kinase kinase kinase 14), Genebank Accession Number: AB209586 (MAPK13, Mitogen-activated protein kinase 13), Genebank Accession Number: NM—005343 (HRAS, V-Ha-ras Harvey rat sarcoma viral oncogene homolog), Genebank Accession Number: BX647104 (FOS, V-fos FBJ murine osteosarcoma viral oncogene homolog), Genebank Accession Number: NM—007315 (STAT1, Signal transducer and activator of transcription 1, 91 kDa), Genebank Accession Number: BC027933 (MAPK11, Mitogen-activated protein kinase 11), Genebank Accession Number: NM—020529 (NFKBIA, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), Genebank Accession Number: BC014966 (RPS6KA1, Ribosomal protein S6 kinase, 90 kDa, polypeptide 1), Genebank Accession Number: NM—181861 (APAF1, Apoptotic peptidase activating factor), Genebank Accession Number: L41870 [RB1, Retinoblastoma 1 (including osteosarcoma)], Genebank Accession Number: CR612719 (GADD45A, Growth arrest and DNA-damage-inducible, alpha), Genebank Accession Number: BM462208 (PCNA, Proliferating cell nuclear antigen), Genebank Accession Number: M92424 [MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)], Genebank Accession Number: NM—078467 [CDKN1A, Cyclin-dependent kinase inhibitor 1A (p21, Cip1)], Genebank Accession Number: BC089389 (IHPK3, Inositol hexaphosphate kinase 3), Genebank Accession Number: NM—001024807 [APLP1, Amyloid beta (A4) precursor-like protein1], Genebank Accession Number: NM—001013398 (IGFBP3, Insulin-like growth factor binding protein 3), Genebank Accession Number: AB209361 [FAS, Fas (TNF receptor superfamily, member 6)], Genebank Accession Number: NM—147780 (CTSB, Cathepsin B), Genebank Accession Number: NM—001165 (BIRC3, Baculoviral IAP repeat-containing 3), Genebank Accession Number: BM557864 (HSPB1, Heat shock 27 kDa protein 1), Genebank Accession Number: AB033060 (PDCD6, Aryl-hydrocarbon receptor repressor), Genebank Accession Number: AK125880 (TP53INP1, Tumor protein p53 inducible nuclear protein 1), Genebank Accession Number: NM—004574 (SEP4, Septin 4), Genebank Accession Number: NM—003820 [TNFRSF14, Tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)], Genebank Accession Number: CN478604 [LGALS7, Lectin, galactoside-binding, soluble, 7 (galectin 7)], Genebank Accession Number: NM—004879 (EI24, Etoposide induced 2.4 mRNA), Genebank Accession Number: NM—003311 (PHLDA2, Pleckstrin homology-like domain, family A, member 2), Genebank Accession Number: NM—003897 (IER3, Immediate early response 3), Genebank Accession Number: AK097284 (TNFAIP8, Tumor necrosis factor, alpha-induced protein 8), Genebank Accession Number: NM—000582 [SPP1, Secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)], Genebank Accession Number: NM—013258 (PYCARD, PYD and CARD domain containing), Genebank Accession Number: NM—002305 [LGALS1, Lectin, galactoside-binding, soluble, 1(galectin 1)], Genebank Accession Number: AF055634 [UNC5C, Unc-5 homolog C (C. elegans)], Genebank Accession Number: CR614015 (CD14, CD14 antigen), Genebank Accession Number: AK129595 (GADD45B, Growth arrest and DNA-damage-inducible, beta), Genebank Accession Number: AF356193 (CARD6, Caspase recruitment domain family, member 6), Genebank Accession Number: BC063043 [PRF1, Perforin 1 (pore forming protein)], Genebank Accession Number: BX537586 [STK17A, Serine/threonine kinase 17a (apoptosis-inducing)], Genebank Accession Number: NM—003842 (TNFRSF10B, Tumor necrosis factor receptor superfamily, member 10b), Genebank Accession Number: AF208043 (IFI16, Interferon, gamma-inducible protein 16), Genebank Accession Number: BX641114 (ANXA4, Annexin A4), Genebank Accession Number: NM—001009552 (PPP2CB, Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform), Genebank Accession Number: BX386171 (CGB5, Chorionic gonadotropin, beta polypeptide 8), Genebank Accession Number: AY358103 (HIP1, Huntingtin interacting protein 1), Genebank Accession Number: BC027949 (P2RX1, Purinergic receptor P2X, ligand-gated ion channel, 1), Genebank Accession Number: BC042844 (CASP10, Caspase 10, apoptosis-related cysteine peptidase), Genebank Accession Number: NM—000733 [CD3E, CD3E antigen, epsilon polypeptide (TiT3 complex)], Genebank Accession Number: NM—033245 (PML, Promyelocytic leukemia), Genebank Accession Number: BC047362 (PHLDA1, Pleckstrin homology-like domain, family A, member 1), Genebank Accession Number: NM—006763 (BTG2, BTG family, member 2), Genebank Accession Number: BC050455 (DDB2, LIM homeobox 3), Genebank Accession Number: BC068535 (TP53AP1, TP53 activated protein 1), Genebank Accession Number: AF033122 (SESN1, Sestrin 1), Genebank Accession Number: AB024313 (POLH, Polymerase (DNA directed), eta), Genebank Accession Number: NM—001012716 (TYMS, Thymidylate synthetase), Genebank Accession Number: BC095402 (ATXN3, Ataxin 3), Genebank Accession Number: BU675073 (PTTG1, Pituitary tumor-transforming 1), Genebank Accession Number: BC073161 (RAD51C, RAD51 homolog C (S. cerevisiae), Genebank Accession Number: NM—001983 [ERCC1, Excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)], Genebank Accession Number: NM—018965 (TREM2, Triggering receptor expressed on myeloid cells 2), Genebank Accession Number: BX649164 (PAI, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1), Genebank Accession Number: NM—006404 [PROCR, Protein C receptor, endothelial (EPCR)], Genebank Accession Number: C035582 (TRIM22, Tripartite motif-containing 22), Genebank Accession Number: NM—006691 (XLKD1, Extracellular link domain containing 1), Genebank Accession Number: NM—015831 [ACHE, Acetylcholinesterase (YT blood group)], Genebank Accession Number: BC045666 (TP53I11, Tumor protein p53 inducible protein 11), Genebank Accession Number: NM—005438 (FOSL1, FOS-like antigen 1), Genebank Accession Number: NM—005755 (EBI3, Epstein-Barr virus induced gene 3), Genebank Accession Number: BM918324 (LY96, Lymphocyte antigen 96), Genebank Accession Number: NM—000022 (ADA, Adenosine deaminase), Genebank Accession Number: NM—004167 (CCL14, Chemokine (C-C motif) ligand 15), Genebank Accession Number: NM—014278 (HSPA4L, Heat shock 70 kDa protein 4-like), Genebank Accession Number: BC006523 (SGK2, Serum/glucocorticoid regulated kinase 2), Genebank Accession Number: NM—000641 (IL11, Interleukin 11), Genebank Accession Number: H18681 [C20orf139, Sulfiredoxin 1 homolog (S. cerevisiae)], Genebank Accession Number: NM—001769 [CD9, CD9 antigen (p24)], Genebank Accession Number: BG564326 (SAA4, Serum amyloid A4, constitutive), Genebank Accession Number: NM—016816 (OAS1, 2′,5′-oligoadenylate synthetase 1, 40/46 kDa), Genebank Accession Number: CB055213 (ICOSLG, Inducible T-cell co-stimulator ligand), Genebank Accession Number: CR591007 (CCL3, Chemokine (C-C motif) ligand 3), Genebank Accession Number: NM—145641 (APOL3, Apolipoprotein L, 3), Genebank Accession Number: AK127663 (PTGES, Prostaglandin E synthase), Genebank Accession Number: NM—001734 (C1S, Complement component 1, s subcomponent), Genebank Accession Number: NM—002260 (KLRC3, Killer cell lectin-like receptor subfamily C, member 2), Genebank Accession Number: NM—001733 (C1R, Complement component 1, r subcomponent), Genebank Accession Number: AB209922 (DNAJB4, DnaJ (Hsp40) homolog, subfamily B, member 4), Genebank Accession Number: NM—201397 (GPX1, Glutathione peroxidase 1), Genebank Accession Number: AK090482 [CXCL12, Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)], Genebank Accession Number: BF662985 (CCL3L1, Chemokine (C-C motif) ligand 3-like 1), Genebank Accession Number: NM—001779 [CD58, CD58 antigen, (lymphocyte function-associated antigen 3)], Genebank Accession Number: NM—005064 (CCL23, Chemokine (C-C motif) ligand 23), Genebank Accession Number: NM—000242 [MBL2, Mannose-binding lectin (protein C) 2, soluble (opsonic defect)], Genebank Accession Number: NM—032036 (FAM14A, Family with sequence similarity 14, member A), Genebank Accession Number: AJ890082 (HSPCA, Heat shock 90 kDa protein 1, alpha), Genebank Accession Number: BC068487 (TLR3, Toll-like receptor 3), Genebank Accession Number: NM—000552 (VWF, Von Willebrand factor), Genebank Accession Number: NM—000132 [F8, Coagulation factor VIII, procoagulant component (hemophilia A)], Genebank Accession Number: AY154461 (NALP6, NACHT, leucine rich repeat and PYD containing 6), Genebank Accession Number: AK127679 (DUSP1, Dual specificity phosphatase 1), Genebank Accession Number: NM—014314 (DDX58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58), Genebank Accession Number: NM—000311 [PRNP, Prion protein (p27-30) (Creutzfeld-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)], Genebank Accession Number: NM—006597 (HSPA8, Heat shock 70 kDa protein 8), Genebank Accession Number: AK092040 (ZCSL2, Zinc finger, CSL-type containing 2), Genebank Accession Number: NM—031483 [ITCH, Itchy homolog E3 ubiquitin protein ligase (mouse)], Genebank Accession Number: U25029 [NR3C1, Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)], Genebank Accession Number: NM—005242 (F2RL1, Coagulation factor II (thrombin) receptor-like 1), Genebank Accession Number: AK172851 (ADORA2B, Adenosine A2b receptor), Genebank Accession Number: BQ053419 (BATF, Basic leucine zipper transcription factor, ATF-like), Genebank Accession Number: BC020698 (CCL20, Chemokine (C-C motif) ligand 20), Genebank Accession Number: NM—001124 (ADM, Adrenomedullin), Genebank Accession Number: NM—153609 (TMPRSS6, Transmembrane protease, serine 6), Genebank Accession Number: NM—004148 (NINJ1, Ninjurin 1), Genebank Accession Number: AF324224 (APOL2, Apolipoprotein L, 2), Genebank Accession Number: BC040494 (DNAJB2, DnaJ (Hsp40) homolog, subfamily B, member 2), Genebank Accession Number: BE512691 [GPX2, Glutathione peroxidase 2 (gastrointestinal)], Genebank Accession Number: NM—001002029 (C4A, Complement component 4B, telomeric), Genebank Accession Number: NM—000732 [CD3D, CD3D antigen, delta polypeptide (TiT3 complex)], Genebank Accession Number: AK223210 [CD79B, CD79B antigen (immunoglobulin-associated beta)], Genebank Accession Number: NM—002309 (LIF, Hypothetical protein MGC20647), Genebank Accession Number: AF031469 (MR1, Major histocompatibility complex, class I-related), Genebank Accession Number: NM—172208 [TAPBP, TAP binding protein (tapasin)], Genebank Accession Number: BC021117 [CSF1, Colony stimulating factor 1 (macrophage)], Genebank Accession Number: NM—001421 [ELF4, E74-like factor 4 (ets domain transcription factor)], Genebank Accession Number: NM—002119 (HLA-DOA, Major histocompatibility complex, class II, DO alpha), Genebank Accession Number: BX648013 [TAP1, Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)], Genebank Accession Number: AL832287 (ZAP70, Zeta-chain (TCR) associated protein kinase 70 kDa), Genebank Accession Number: BF569086 (NK4, Interleukin 32), Genebank Accession Number: AL832451 (DKFZp451C2311, guanylate binding protein 2, interferon-inducible), Genebank Accession Number: AF069493 (SEMA7A, Sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A), Genebank Accession Number: NM—001008490 (KLF6, Kruppel-like factor 6), Genebank Accession Number: NM—000418 (IL4R, Interleukin 4 receptor), Genebank Accession Number: NM—004048 (B2M, Beta-2-microglobulin), Genebank Accession Number: AF116720 (DNAJA2, DnaJ (Hsp40) homolog, subfamily A, member 2), Genebank Accession Number: NM—001007271 (DUSP13, Dual specificity phosphatase 13), Genebank Accession Number: BM904612 [S100A6, S100 calcium binding protein A6 (calcyclin)], Genebank Accession Number: AK074652 (SERTAD1, SERTA domain containing 1), Genebank Accession Number: NM—203394 (E2F7, E2F transcription factor 7), Genebank Accession Number: NM—003620 (PPM1D, Protein phosphatase 1D magnesium-dependent, delta isoform), Genebank Accession Number: BC060797 [PARD6G, Par-6 partitioning defective 6 homolog gamma (C. elegans)], Genebank Accession Number: NM—021913 (AXL, AXL receptor tyrosine kinase), Genebank Accession Number: BC040303 (PTP4A1, Protein tyrosine phosphatase type IVA, member 1), Genebank Accession Number: BC008564 (PPP1R9B, Protein phosphatase 1, regulatory subunit 9B, spinophilin), Genebank Accession Number: AK097024 [RAE1, RAE1 RNA export 1 homolog (S. pombe)], Genebank Accession Number: BC011957 (BTG3, BTG family, member 3), Genebank Accession Number: NM—078487 [CDKN2B, Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)], Genebank Accession Number: BC023552 (SFN, Stratifin), Genebank Accession Number: BC060847 [PARD6B, Par-6 partitioning defective 6 homolog beta (C. elegans)], Genebank Accession Number: NM—003236 (TGFA, Transforming growth factor, alpha), Genebank Accession Number: NM—002632 (PGF, Placental growth factor, vascular endothelial growth factor-related protein), Genebank Accession Number: NM—015278 (SASH1, SAM and SH3 domain containing 1), Genebank Accession Number: AL834276 (MGC3207, Hypothetical protein MGC3207), Genebank Accession Number: AK096276 (CCND3, Cyclin D3), Genebank Accession Number: AB209373 (CCNK, Chromosome 14 open reading frame 65), Genebank Accession Number: NM—021132 [PPP3CB, Protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)], Genebank Accession Number: NM—005072 (SLC12A4, Solute carrier family 12 (potassium/chloride transporters), member 4), Genebank Accession Number: BX647886 [PCNP, SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae)], Genebank Accession Number: NM—044472 [CDC42, Cell division cycle 42 (GTP binding protein, 25 kDa)], Genebank Accession Number: AL832973 [REC8L1, REC8-like 1 (yeast)], Genebank Accession Number: NM—002031 (FRK, Fyn-related kinase), Genebank Accession Number: BM913048 (TIMP1, TIMP metallopeptidase inhibitor 1), Genebank Accession Number: NM—003975 (SH2D2A, SH2 domain protein 2A), Genebank Accession Number: NM—198129 (LAMA3, Laminin, alpha 3), Genebank Accession Number: NM—006152 (LRMP, Lymphoid-restricted membrane protein), Genebank Accession Number: X96753 [CSPG4, Chondroitin sulfate proteoglycan 4 (melanoma-associated)], Genebank Accession Number: NM—000474 [TWIST1, Twist homolog 1(acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)], Genebank Accession Number: AF052152 (DCAMKL1, Doublecortin and CaM kinase-like 1), Genebank Accession Number: AF333334 (CCIN, Calicin), Genebank Accession Number: NM—005979 (S100A13, S100 calcium binding protein A13), Genebank Accession Number: NM—004429 (EFNB1, Ephrin-B1), Genebank Accession Number: NM—003873 (NRP1, Neuropilin 1), Genebank Accession Number: AF026692 (SFRP4, Secreted frizzled-related protein 4), Genebank Accession Number: AK124583 (DAZAP1, DAZ associated protein 1), Genebank Accession Number: AF003837 [JAG1, Jagged 1 (Alagille syndrome)], Genebank Accession Number: AB209590 (BMP1, Bone morphogenetic protein 1), Genebank Accession Number: X54457 [CEL, Carboxyl ester lipase (bile salt-stimulated lipase)], Genebank Accession Number: Y11307 (CYR61, Cysteine-rich, angiogenic inducer, 61), Genebank Accession Number: NM—000596 (IGFBP1, Insulin-like growth factor binding protein 1), Genebank Accession Number: NM—020997 (LEFTY1, Left-right determination factor 1), Genebank Accession Number: NM—001553 (IGFBP7, Insulin-like growth factor binding protein 7), Genebank Accession Number: AB209509 (IGFBP2, Insulin-like growth factor binding protein 2, 36 kDa), Genebank Accession Number: NM—001552 (IGFBP4, Insulin-like growth factor binding protein 4), Genebank Accession Number: AB209321 (CSRP2, Cysteine and glycine-rich protein 2), Genebank Accession Number: BM556279 (EMP3, Epithelial membrane protein 3), Genebank Accession Number: NM—005727 (TSPAN1, Tetraspanin 1), Genebank Accession Number: BQ683841 [S100A11, S100 calcium binding protein A11 (calgizzarin)], Genebank Accession Number: BC042390 [VTI1B, Vesicle transport through interaction with t-SNAREs homolog 1B (yeast)], Genebank Accession Number: NM—000899 (KITLG, KIT ligand), Genebank Accession Number: BC032940 (LAMP3, Lysosomal-associated membrane protein 3), Genebank Accession Number: BQ939577 [AKR1C3, Aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)], Genebank Accession Number: BC063468 (OSMR, Oncostatin M receptor), Genebank Accession Number: CR936719 [SSR1, Signal sequence receptor, alpha (translocon-associated protein alpha)], Genebank Accession Number: AB024057 [TBC1D8, TBC1 domain family, member 8 (with GRAM domain)], Genebank Accession Number: NM—003329 (TXN, Thioredoxin), Genebank Accession Number: NM—012125 (CHRM5, Cholinergic receptor, muscarinic 5), Genebank Accession Number: NM—007045 (FGFR1OP, FGFR1 oncogene partner), Genebank Accession Number: AK094474 (RHOC, Ras homolog gene family, member C), Genebank Accession Number: BC017706 (6PGL, 6-phosphogluconolactonase), Genebank Accession Number: AF153821 (ADH1B, Alcohol dehydrogenase IB (class I), beta polypeptide), Genebank Accession Number: AK124656 [ENO2, Enolase 2 (gamma, neuronal)], Genebank Accession Number: D42047 (GPD1L, Glycerol-3-phosphate dehydrogenase 1-like), Genebank Accession Number: AK130060 (TALDO1, Transaldolase 1), Genebank Accession Number: AL833069 (KIAA1434, Hypothetical protein KIAA1434), Genebank Accession Number: XM—496436 (UNQ6077, C219-reactive peptide), Genebank Accession Number: NM—199186 (BPGM, 2,3-bisphosphoglycerate mutase), Genebank Accession Number: U86136 (TEP1, Telomerase-associated protein 1), Genebank Accession Number: BU603483 (HIST1H1E, Histone 1, H1e), Genebank Accession Number: NM—005319 (HIST1H1C, Histone 1, H1c), Genebank Accession Number: NM—005320 (HIST1H1D, Histone 1, H1d), Genebank Accession Number: NM—003516 (HIST2H2AA, Histone 2, H2aa), Genebank Accession Number: F02250 (HDAC2, Histone deacetylase 2), Genebank Accession Number: NM—004739 (MTA2, Metastasis associated 1 family, member 2), Genebank Accession Number: BC042589 (HIST1H2BD, Histone 1, H2bd), Genebank Accession Number: CB529694 (HIST2H2BE, Histone 2, H2be), Genebank Accession Number: AK126154 (NP, Nucleoside phosphorylase), Genebank Accession Number: AB040899 (KIAA1466, KIAA1466 gene), Genebank Accession Number: CR608156 (HIST1H2AC, Histone 1, H2ac), Genebank Accession Number: BX436525 (HIST1H2BH, Histone 1, H2bh), Genebank Accession Number: AK057762 (TDH, L-threonine dehydrogenase), Genebank Accession Number: BC035124 (UNQ2541, MSFL2541), Genebank Accession Number: BC034763 (FDXR, Ferredoxin reductase), Genebank Accession Number: AK093461 (ISYNA1, Myo-inositol 1-phosphate synthase A1), Genebank Accession Number: AK097009 (PLTP, phospholipid transfer protein), Genebank Accession Number: AF524864 [AKR1B10, Aldo-keto reductase family 1, member B10 (aldose reductase)], Genebank Accession Number: NM—021727 (FADS3, Fatty acid desaturase 3), Genebank Accession Number: CR627415 (HSD3B2, Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2), Genebank Accession Number: CD013987 (CYP21A2, Cytochrome P450, family 21, subfamily A, polypeptide 2), Genebank Accession Number: BC020744 (AKR1C4, Aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol), dehydrogenase 4), Genebank Accession Number: NM—004753 (DHRS3, Dehydrogenase/reductase (SDR family) member 3), Genebank Accession Number: BC030207 (NR0B2, Nuclear receptor subfamily 0, group B, member 2), Genebank Accession Number: AK091948 (LTB4DH, Leukotriene B4 12-hydroxydehydrogenase), Genebank Accession Number: NM—002900 (RBP3, Retinol binding protein 3, interstitial), Genebank Accession Number: BX647927 (PLCD1, Phospholipase C, delta 1), Genebank Accession Number: NM—145323 (OSBPL3, Oxysterol binding protein-like 3), Genebank Accession Number: NM—000169 (GLA, Galactosidase, alpha), Genebank Accession Number: BC094756 (CRA, Myotubularin related protein 11), Genebank Accession Number: AB043587 (ACBD3, Acyl-Coenzyme A binding domain containing 3), Genebank Accession Number: NM—001478 [GALGT, UDP-N-acetyl-alpha-D-galactosamine: (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase (GalNAc-T)], Genebank Accession Number: J05428 (UGT2B7, UDP glucuronosyltransferase 2 family, polypeptide B7), Genebank Accession Number: AB002454 (CYP4F3, Cytochrome P450, family 4, subfamily F, polypeptide 3), Genebank Accession Number: NM—000405 (GM2A, GM2 ganglioside activator), Genebank Accession Number: BC001823 (PRKAB1, Protein kinase, AMP-activated, beta 1 non-catalytic subunit), Genebank Accession Number: NM—145343 (APOL1, Apolipoprotein L, 1), Genebank Accession Number: NM—006571 (DCTN6, Dynactin 6), Genebank Accession Number: NM—020139 (DHRS6, Dehydrogenase/reductase (SDR family) member 6), Genebank Accession Number: NM—000499 (CYP1A1, Cytochrome P450, family 1, subfamily A, polypeptide 1), Genebank Accession Number: BC007003 [TGM4, Transglutaminase 4 (prostate)], Genebank Accession Number: NM—012189 [CABYR, Calcium binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2)], Genebank Accession Number: CR615530 (CYGB, Cytoglobin), Genebank Accession Number: NM—004199 (P4HA2, Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II), Genebank Accession Number: AK027560 (CYP26A1, Cytochrome P450, family 26, subfamily A, polypeptide 1), Genebank Accession Number: NM—145298 (APOBEC3F, Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F), Genebank Accession Number: NM—005576 (LOXL1, Lysyl oxidase-like 1), Genebank Accession Number: AK024854 [APOBEC3B, Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D (putative)], Genebank Accession Number: NM—014301 (NIFUN, NifU-like N-terminal domain containing), Genebank Accession Number: BF670653 (MB, Myoglobin), Genebank Accession Number: AK126982 (LHX6, LIM homeobox 6), Genebank Accession Number: Y13786 [ADAM19, ADAM metallopeptidase domain 19 (meltrin beta)], Genebank Accession Number: AB051455 (MICAL-L1, MICAL-like 1), Genebank Accession Number: AK074068 (MICAL-L2, MICAL-like 2), Genebank Accession Number: AK124421 [CPA1, Carboxypeptidase A1 (pancreatic)], Genebank Accession Number: NM—030622 (CYP2S1, Cytochrome P450, family 2, subfamily S, polypeptide 1), Genebank Accession Number: BC012027 (CYP2U1, Cytochrome P450, family 2, subfamily U, polypeptide 1), Genebank Accession Number: AY461717 (ZNF385, Zinc finger protein 385), Genebank Accession Number: AK092424 (ZSCAN4, Zinc finger and SCAN domain containing 4), Genebank Accession Number: AB208935 [RASAL1, RAS protein activator like 1 (GAP1 like)], Genebank Accession Number: NM—201444 (DGKA, Diacylglycerol kinase, alpha 80 kDa), Genebank Accession Number: BC035625 [EGR2, Early growth response 2 (Krox-20 homolog, Drosophila)], Genebank Accession Number: BF983391 (RPS27L, Ribosomal protein S27-like), Genebank Accession Number: BC036545 (PLAC2, Placenta-specific 2), Genebank Accession Number: BC034569 (ZNF337, Zinc finger protein 337), Genebank Accession Number: CR936707 (TRIM38, Tripartite motif-containing 38), Genebank Accession Number: AK091940 (MGC4734, Ring finger protein 183), Genebank Accession Number: NM—024101 (MLPH, Melanophilin), Genebank Accession Number: CR601823 (HIVEP3, Human immunodeficiency virus type I enhancer binding protein 3), Genebank Accession Number: BC041578 (MGC17986, zinc finger protein 114), Genebank Accession Number: BC038432 (RARA, Retinoic acid receptor, alpha), Genebank Accession Number: BX641053 (FXYD2, FXYD domain containing ion transport regulator 2), Genebank Accession Number: AK095363 (AQP3, Aquaporin 3), Genebank Accession Number: AK074100 (SLC37A2, Solute carrier family 37 (glycerol-3-phosphate transporter), member 2), Genebank Accession Number: AK024002 (TUBA4, Tubulin, alpha 4), Genebank Accession Number: AK092677 (TUBB6, Tubulin, beta 6), Genebank Accession Number: NM—001297 (CNGB1, Cyclic nucleotide gated channel beta 1), Genebank Accession Number: NM—032872 (SYTL1, Synaptotagmin-like 1), Genebank Accession Number: NM—014045 (LRP10, Low density lipoprotein receptor-related protein 10), Genebank Accession Number: NM—014585 (SLC40A1, Solute carrier family 40 (iron-regulated transporter), member 1), Genebank Accession Number: L02870 [COL7A1, Collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive)], Genebank Accession Number: AK054731 [TUBA1, Tubulin, alpha 1 (testis specific)], Genebank Accession Number: AF336127 (SLC4A11, Solute carrier family 4, sodium bicarbonate transporter-like, member 11), Genebank Accession Number: AB075951 (SLC22A2, Solute carrier family 22 (organic cation transporter), member 2), Genebank Accession Number: AK125026 (SLC6A12, Solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12), Genebank Accession Number: BX537541 (MGC13186, Chromosome 1 open reading frame 57), Genebank Accession Number: CD013956 (SHBG, Sex hormone-binding globulin), Genebank Accession Number: BC029057 (DAO, D-amino-acid oxidase), Genebank Accession Number: BC041479 (COL9A2, Collagen, type IX, alpha 2), Genebank Accession Number: AL050210 (LOC283130, Hypothetical protein LOC283130), Genebank Accession Number: NM—004798 (KIF3B, Kinesin family member 3B), Genebank Accession Number: AK025742 [UCP2, Uncoupling protein 2 (mitochondrial, proton carrier)], Genebank Accession Number: NM—021990 (GABRE, Gamma-aminobutyric acid (GABA) A receptor, epsilon), Genebank Accession Number: NM—005819 (STX6, Syntaxin 6), Genebank Accession Number: BC092420 [CYP4F11, Similar to Cytochrome P450 4F12 (CYPIVF12) (UNQ568/PRO1129)], Genebank Accession Number: BC043423 (ABCB6, ATP-binding cassette, sub-family B (MDR/TAP), member 6), Genebank Accession Number: BC021025 (CTHRC1, Collagen triple helix repeat containing 1), Genebank Accession Number: NM—004999 (MYO6, Myosin VI), Genebank Accession Number: AF445025 (SLC35A4, Solute carrier family 35, member A4), Genebank Accession Number: NM—003330 (TXNRD1, Thioredoxin reductase 1), Genebank Accession Number: AK092512 (SLC16A5, Solute carrier family 16 (monocarboxylic acid transporters), member 5), Genebank Accession Number: AB075871 [FLJ42654, Golgi transport 1 homolog A (S. cerevisiae)], Genebank Accession Number: NM—004864 (GDF15, Growth differentiation factor 15), Genebank Accession Number: NM—022153 (PP2135, Chromosome 10 open reading frame 54), Genebank Accession Number: NM—001013632 (LOC343521, Similar to hypothetical protein 4833401D15), Genebank Accession Number: AK124751 (CAPN2, Calpain 2, (m/II) large subunit), Genebank Accession Number: AK095079 (RGC32, Response gene to complement 32), Genebank Accession Number: NM—031286 (SH3BGRL3, SH3 domain binding glutamic acid-rich protein like 3), Genebank Accession Number: AK126182 (KIAA1949, KIAA1949), Genebank Accession Number: NM—080388 (S100A16, S100 calcium binding protein A16), Genebank Accession Number: NM—025106 (SSB1, SplA/ryanodine receptor domain and SOCS box containing 1), Genebank Accession Number: NM—203403 (C9orf150, Chromosome 9 open reading frame 150), Genebank Accession Number: AK096241 (SQSTM1, Sequestosome 1), Genebank Accession Number: BQ276852 (TMSB10, Thymosin, beta 10), Genebank Accession Number: BC101655 (HIST1H2BI, Histone 1, H2bi), Genebank Accession Number: W84524 (TM4SF5, Transmembrane 4 L six family member 5), Genebank Accession Number: BX647290 (HIST1H2BO, Histone 1, H2bo), Genebank Accession Number: NM—002467 [MYC, V-myc myelocytomatosis viral oncogene homolog (avian)], Genebank Accession Number: BC038295 (MAP2K7, Mitogen-activated protein kinase kinase 7), Genebank Accession Number: NM—004364 (CEBPA, CCAAT/enhancer binding protein (C/EBP), alpha), Genebank Accession Number: NM—145161 (MAP2K5, Mitogen-activated protein kinase kinase 5), Genebank Accession Number: XM—042066 (MAP3K1, Mitogen-activated protein kinase kinase kinase 1), Genebank Accession Number: Z25424 (MKNK2, MAP kinase interacting serine/threonine kinase 2), Genebank Accession Number: AB209047 (ELK1, ELK1, member of ETS oncogene family), Genebank Accession Number: NM—004755 (RPS6KA5, Ribosomal protein S6 kinase, 90 kDa, polypeptide 5), Genebank Accession Number: AB001872 (RPL4, Mitogen-activated protein kinase kinase kinase 13), Genebank Accession Number: NM—002758 (MAP2K6, Mitogen-activated protein kinase kinase 6), Genebank Accession Number: AK098095 (BRAF, V-raf murine sarcoma viral oncogene homolog B1), Genebank Accession Number: AB208919 [FGFR1, Fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)], Genebank Accession Number: BC060837 (DUSP9, Dual specificity phosphatase 9), Genebank Accession Number: NM—000142 [FGFR3, Fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism)], Genebank Accession Number: AY247738 [TRIB3, Tribbles homolog 3 (Drosophila)], Genebank Accession Number: NM—030640 (DUSP16, Dual specificity phosphatase 16), Genebank Accession Number: NM—148957 (TNFRSF19, Tumor necrosis factor receptor superfamily, member 19), Genebank Accession Number: AB011123 (TNIK, TRAF2 and NCK interacting kinase), Genebank Accession Number: NM—006549 (CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, beta), Genebank Accession Number: NM—022740 (HIPK2, Homeodomain interacting protein kinase 2), Genebank Accession Number: NM—005400 (PRKCE, Protein kinase C, epsilon), Genebank Accession Number: NM—000893 (KNG1, Kininogen 1), Genebank Accession Number: NM—000066 (C8B, Complement component 8, beta polypeptide), Genebank Accession Number: CR749293 (PLG, Plasminogen), Genebank Accession Number: BC013780 (ADORA2A, Adenosine A2a receptor), Genebank Accession Number: NM—001012271 (BIRC5, Effector cell peptidase receptor 1), Genebank Accession Number: NM—003811 (TNFSF9, Tumor necrosis factor (ligand) superfamily, member 9), Genebank Accession Number: BC050369 (E2F1, E2F transcription factor 1), Genebank Accession Number: NM—007313 (ABL1, V-abl Abelson murine leukemia viral oncogene homolog 1), Genebank Accession Number: BX647151 (MYBL2, V-myb myeloblastosis viral oncogene homolog (avian)-like 2), Genebank Accession Number: NM—004822 (NTN1, Netrin 1), Genebank Accession Number: BC033694 [BCL2L11, BCL2-like 11 (apoptosis facilitator)], Genebank Accession Number: AB208876 (AXIN1, Axin 1), Genebank Accession Number: BC051332 [F2, Coagulation factor II (thrombin)], Genebank Accession Number: AK122614 (PPARD, Peroxisome proliferative activated receptor, delta), Genebank Accession Number: L78790 [ITGB2, Integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)], Genebank Accession Number: AK223409 (BARD1, BRCA1 associated RING domain 1), Genebank Accession Number: NM—002737 (PRKCA, Protein kinase C, alpha), Genebank Accession Number: NM—012479 (YWHAG, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide), Genebank Accession Number: CR596268 [ITGB3BP, Integrin beta 3 binding protein (beta 3-endonexin)], Genebank Accession Number: NM—003551 [NME5, Non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)], Genebank Accession Number: NM—013974 (DDAH2, Dimethylarginine dimethylaminohydrolase 2), Genebank Accession Number: D79987 [ESPL1, Extra spindle poles like 1 (S. cerevisiae)], Genebank Accession Number: NM—006427 (SIVA, CD27-binding (Siva) protein), Genebank Accession Number: NM—024900 (PHF17, PHD finger protein 17), Genebank Accession Number: AB046854 (MAGI-3, Membrane associated guanylate kinase, WW and PDZ domain containing 3), Genebank Accession Number: AK095578 (SPHK1, Sphingosine kinase 1), Genebank Accession Number: AK122762 (CIDEA, Cell death-inducing DFFA-like effector a), Genebank Accession Number: AK092872 [ERCC2, Excision repair cross-complementing rodent repair deficiency, complementation group 2 (xeroderma pigmentosum D)], Genebank Accession Number: NM—006290 (TNFAIP3, Tumor necrosis factor, alpha-induced protein 3), Genebank Accession Number: NM—000562 (C8A, Complement component 8, alpha polypeptide), Genebank Accession Number: AK122894 (AKT1, V-akt murine thymoma viral oncogene homolog 1), Genebank Accession Number: NM—005067 [SIAH2, Seven in absentia homolog 2 (Drosophila)], Genebank Accession Number: NM—203339 [CLU, Clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)], Genebank Accession Number: NM—003376 (VEGF, Vascular endothelial growth factor), Genebank Accession Number: AK027590 (DAPK3, Death-associated protein kinase 3), Genebank Accession Number: AK122825 (HMGB1, High-mobility group box 1), Genebank Accession Number: NM—006904 (PRKDC, Protein kinase, DNA-activated, catalytic polypeptide), Genebank Accession Number: AK074377 (UHRF1, Chromosome 19 open reading frame 31), Genebank Accession Number: CR604810 (CCNA2, Cyclin A2), Genebank Accession Number: AK001720 [FLJ10858, Nei endonuclease VIII-like 3 (E. coli)], Genebank Accession Number: CR600021 (HMGB2, High-mobility group box 2), Genebank Accession Number: NM—006230 (POLD2, Polymerase (DNA directed), delta 2, regulatory subunit 50 kDa), Genebank Accession Number: NM—018151 [RIF1, RAP1 interacting factor homolog (yeast)], Genebank Accession Number: BX248766 [RAD51L1, RAD51-like 1 (S. cerevisiae)], Genebank Accession Number: NM—014502 [PRP19, PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)], Genebank Accession Number: NM—013975 (LIG3, Ligase III, DNA, ATP-dependent), Genebank Accession Number: NM—173627 (FLJ35220, Hypothetical protein FLJ35220), Genebank Accession Number: NM—003362 (UNG, Uracil-DNA glycosylase), Genebank Accession Number: AF076494 (IRF7, Interferon regulatory factor 7), Genebank Accession Number: CR625391 (RAD51AP1, RAD51 associated protein 1), Genebank Accession Number: NM—006231 (POLE, Polymerase (DNA directed), epsilon), Genebank Accession Number: NM—130398 (EXO1, Exonuclease 1), Genebank Accession Number: DQ140356 (KIAA1596, Fanconi anemia, complementation group M), Genebank Accession Number: NM—002105 (H2AFX, H2A histone family, member X), Genebank Accession Number: AB209560 (POLD1, Polymerase (DNA directed), delta 1, catalytic subunit 125 kDa), Genebank Accession Number: NM—000136 (FANCC, Fanconi anemia, complementation group C), Genebank Accession Number: NM—003368 (USP1, Ubiquitin specific peptidase 1), Genebank Accession Number: NM—022836 [DCLRE1B, DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)], Genebank Accession Number: NM—001067 (TOP2A, Topoisomerase (DNA) II alpha 170 kDa), Genebank Accession Number: NM—002913 (RFC1, Replication factor C (activator 1) 1, 145 kDa), Genebank Accession Number: NM—002412 (MGMT, O-6-methylguanine-DNA methyltransferase), Genebank Accession Number: NM—000251 [MSH2, MutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)], Genebank Accession Number: NM—015254 (KIF13B, Kinesin family member 13B), Genebank Accession Number: NM—006037 (HDAC4, Histone deacetylase 4), Genebank Accession Number: NM—020995 (HPR, Haptoglobin-related protein), Genebank Accession Number: NM—153443 (KIR3DL1, Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1), Genebank Accession Number: BC071718 (CCL4L1, Chemokine (C-C motif) ligand 4-like 1), Genebank Accession Number: NM—020979 (APS, Adaptor protein with pleckstrin homology and src homology 2 domains), Genebank Accession Number: NM—001629 (ALOX5AP, Arachidonate 5-lipoxygenase-activating protein), Genebank Accession Number: NM—130852 (PLUNC, Palate, lung and nasal epithelium carcinoma associated), Genebank Accession Number: AK024499 (SPON2, Spondin 2, extracellular matrix protein), Genebank Accession Number: BC048198 (AHSG, Alpha-2-HS-glycoprotein), Genebank Accession Number: AK095849 (RNU22, RNA, U22 small nucleolar), Genebank Accession Number: BC005395 (HPX, Hemopexin), Genebank Accession Number: NM—012276 (ILT7, Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4), Genebank Accession Number: NM—003152 (STAT5A, Signal transducer and activator of transcription 5A), Genebank Accession Number: BC037171 (CCL27, Chemokine (C-C motif) ligand 27), Genebank Accession Number: NM—002468 [MYD88, Myeloid differentiation primary response gene (88)], Genebank Accession Number: NM—002215 (ITIH1, Inter-alpha (globulin) inhibitor H1), Genebank Accession Number: NM—004750 (CRLF1, Cytokine receptor-like factor 1), Genebank Accession Number: DR004094 (HAMP, Hepcidin antimicrobial peptide), Genebank Accession Number: BC063245 (GNLY, Granulysin), Genebank Accession Number: AJ290445 (SARM1, Sterile alpha and TIR motif containing 1), Genebank Accession Number: AK122686 [IF, I factor (complement)], Genebank Accession Number: AB008535 (GPR44, G protein-coupled receptor 44), Genebank Accession Number: AK122912 (ZGPAT, Zinc finger, CCCH-type with G patch domain), Genebank Accession Number: BC022312 (C4BPA, Complement component 4 binding protein, alpha), Genebank Accession Number: BG564683 (ORM1, Orosomucoid 1), Genebank Accession Number: NM—000625 [NOS2A, Nitric oxide synthase 2A (inducible, hepatocytes)], Genebank Accession Number: NM—005515 (HLXB9, Homeo box HB9), Genebank Accession Number: BX537504 (C2, Complement component 2), Genebank Accession Number: BC028153 (LILRB3, Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3), Genebank Accession Number: AF130100 (SERPINC1, Serpin peptidase inhibitor, clade C (antithrombin), member 1), Genebank Accession Number: BC015110 (HHEX, Hematopoietically expressed homeobox), Genebank Accession Number: AB209179 [PLK1, Polo-like kinase 1 (Drosophila)], Genebank Accession Number: AY367065 [ASPM, Asp (abnormal spindle)-like, microcephaly associated (Drosophila)], Genebank Accession Number: NM—006101 (KNTC2, Kinetochore associated 2), Genebank Accession Number: NM—003550 [MAD1L1, MAD1 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—020242 (KNSL7, Kinesin family member 15), Genebank Accession Number: AB033068 [FZR1, Fizzy/cell division cycle 20 related 1 (Drosophila)], Genebank Accession Number: NM—018492 (PBK, PDZ binding kinase), Genebank Accession Number: NM—003318 (TTK, TTK protein kinase), Genebank Accession Number: BC011000 (CDCA5, Cell division cycle associated 5), Genebank Accession Number: NM—003981 (PRC1, Protein regulator of cytokinesis 1), Genebank Accession Number: NM—015097 (CLASP2, Cytoplasmic linker associated protein 2), Genebank Accession Number: U65410 [MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—016343 [CENPF, Centromere protein F, 350/400 ka (mitosin)], Genebank Accession Number: NM—005504 (BCAT1, Branched chain aminotransferase 1, cytosolic), Genebank Accession Number: NM—003718 [CDC2L5, Cell division cycle 2-like 5 (cholinesterase-related cell division controller)], Genebank Accession Number: NM—002768 (PCOLN3, Procollagen (type III) N-endopeptidase), Genebank Accession Number: BC004352 (KIF22, Kinesin family member 22), Genebank Accession Number: NM—005983 [SKP2, S-phase kinase-associated protein 2 (p45)], Genebank Accession Number: NM—004523 (KIF11, Kinesin family member 11), Genebank Accession Number: CR592757 [BRRN1, Barren homolog 1 (Drosophila)], Genebank Accession Number: NM—019063 (EML4, Echinoderm microtubule associated protein like 4), Genebank Accession Number: NM—006845 (KIF2C, Kinesin family member 2C), Genebank Accession Number: NM—004701 (CCNB2, Cyclin B2), Genebank Accession Number: NM—138555 (KIF23, Kinesin family member 23), Genebank Accession Number: NM—182687 (PKMYT1, Protein kinase, membrane associated tyrosine/threonine 1), Genebank Accession Number: NM—001790 (CDC25C, Cell division cycle 25C), Genebank Accession Number: BC032677 (UBE2C, Ubiquitin-conjugating enzyme E2C), Genebank Accession Number: NM—005886 (KATNB1, Katanin p80 (WD repeat containing) subunit B 1), Genebank Accession Number: BC073878 (KIFC1, Kinesin family member C1), Genebank Accession Number: AL833191 [SMC2L1, SMC2 structural maintenance of chromosomes 2-like 1 (yeast)], Genebank Accession Number: AF053305 [BUB1, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)], Genebank Accession Number: Z25431 (NEK1, NIMA (never in mitosis gene a)-related kinase 1), Genebank Accession Number: BC043371 [GFI1B, Growth factor independent 1B (potential regulator of CDKN1A, translocated in CML)], Genebank Accession Number: CR933728 (CDC2, Cell division cycle 2, G1 to S and G2 to M), Genebank Accession Number: NM—014750 [DLG7, Discs, large homolog 7 (Drosophila)], Genebank Accession Number: NM—018560 (WWOX, WW domain containing oxidoreductase), Genebank Accession Number: BG203702 (FHIT, Nasopharyngeal carcinoma, down-regulated 1), Genebank Accession Number: BC050384 [SUPT3H, Suppressor of Ty 3 homolog (S. cerevisiae)], Genebank Accession Number: NM—000189 (HK2, Hexokinase 2), Genebank Accession Number: CD049340 (AURKB, Aurora kinase B), Genebank Accession Number: AB011153 [PLCB1, Phospholipase C, beta 1 (phosphoinositide-specific)], Genebank Accession Number: AB209271 (ERF, Ets2 repressor factor), Genebank Accession Number: NM—014264 [PLK4, Polo-like kinase 4 (Drosophila)], Genebank Accession Number: NM—002417 (MKI67, Antigen identified by monoclonal antibody Ki-67), Genebank Accession Number: XM—496557 (RGP1, Plasminogen-like B2), Genebank Accession Number: NM—170715 (RASSF1, Ras association (RalGDS/AF-6) domain family 1), Genebank Accession Number: NM—004526 [MCM2, MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)], Genebank Accession Number: BC033086 [TCF19, Transcription factor 19 (SC1)], Genebank Accession Number: NM—001007071 (RPS6KB2, Ribosomal protein S6 kinase, 70 kDa, polypeptide 2), Genebank Accession Number: NM—016272 (TOB2, Transducer of ERBB2, 2), Genebank Accession Number: AK126688 (CDYL2, Chromodomain protein, Y-like 2), Genebank Accession Number: NM—001759 (CCND2, Cyclin D2), Genebank Accession Number: NM—002388 [MCM3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)], Genebank Accession Number: NM—182776 [MCM7, MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)], Genebank Accession Number: NM—012145 [DTYMK, Deoxythymidylate kinase (thymidylate kinase)], Genebank Accession Number: NM—014751 (MTSS1, Metastasis suppressor 1), Genebank Accession Number: NM—024342 (GRLF1, Glucocorticoid receptor DNA binding factor 1), Genebank Accession Number: AK025627 (CABLES1, Cdk5 and Abl enzyme substrate 1), Genebank Accession Number: NM—005915 [MCM6, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae)], Genebank Accession Number: AF196185 [PARD3, Par-3 partitioning defective 3 homolog (C. elegans)], Genebank Accession Number: NM—019001 (XRN1, 5′-3′ exoribonuclease 1), Genebank Accession Number: NM—006739 [MCM5, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)], Genebank Accession Number: NM—012218 (ILF3, Interleukin enhancer binding factor 3, 90 kDa), Genebank Accession Number: AB053172 (CDT1, DNA replication factor), Genebank Accession Number: AK074112 (CCNL2, Cyclin L2), Genebank Accession Number: NM—001005414 (ZWINT, ZW10 interactor antisense), Genebank Accession Number: BC033391 (PP1665, Glycerophosphodiester phosphodiesterase domain containing 5), Genebank Accession Number: NM—000672 [ADH6, Alcohol dehydrogenase 6 (class V)], Genebank Accession Number: NM—004563 [PCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial)], Genebank Accession Number: XM—113962 (KIAA0650, Structural maintenance of chromosomes flexible hinge domain containing 1), Genebank Accession Number: NM—001500 (GMDS, GDP-mannose 4,6-dehydratase), Genebank Accession Number: NM—002541 [OGDH, Oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)], Genebank Accession Number: NM—000298 (PKLR, Pyruvate kinase, liver and RBC), Genebank Accession Number: NM—005276 [GPD1, Glycerol-3-phosphate dehydrogenase 1 (soluble)], Genebank Accession Number: AB209127 (PFKL, Phosphofructokinase, liver), Genebank Accession Number: M15943 (ADH4, Alcohol dehydrogenase 4 (class II), pi polypeptide), Genebank Accession Number: NM—000671 (ADH5, Alcohol dehydrogenase 5 (class III), chi polypeptide), Genebank Accession Number: BC026320 (OGDHL, Oxoglutarate dehydrogenase-like), Genebank Accession Number: NM—009590 [AOC2, Amine oxidase, copper containing 2 (retina-specific)], Genebank Accession Number: BC015797 (SLC25A10, Solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10), Genebank Accession Number: NM—003748 (ALDH4A1, Aldehyde dehydrogenase 4 family, member A1), Genebank Accession Number: NM—002108 (HAL, Histidine ammonia-lyase), Genebank Accession Number: NM—003486 (SLC7A5, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 5), Genebank Accession Number: NM—003045 (SLC7A1, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 1), Genebank Accession Number: NM—000277 (PAH, Phenylalanine hydroxylase), Genebank Accession Number: NM—012331 (MSRA, Methionine sulfoxide reductase A), Genebank Accession Number: BC008366 [DDC, Dopa decarboxylase (aromatic L-amino acid decarboxylase)], Genebank Accession Number: NM—006567 [FARS2, Phenylalanine-tRNA synthetase 2 (mitochondrial)], Genebank Accession Number: AK055862 (FLJ31300, Urocanase domain containing 1), Genebank Accession Number: NM—001875 (CPS1, Carbamoyl-phosphate synthetase 1, mitochondrial), Genebank Accession Number: AK122685 (GLUD1, Glutamate dehydrogenase 1), Genebank Accession Number: NM—003046 (SLC7A2, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 2), Genebank Accession Number: AK093306 (PHGDH, Phosphoglycerate dehydrogenase), Genebank Accession Number: NM—080820 (HARS2, Histidyl-tRNA synthetase 2), Genebank Accession Number: NM—006907 (PYCR1, Pyrroline-5-carboxylate reductase 1), Genebank Accession Number: NM—020117 (LARS, Leucyl-tRNA synthetase), Genebank Accession Number: BC051726 [GLUL, Glutamate-ammonia ligase (glutamine synthetase)], Genebank Accession Number: BC012616 (BHMT, Betaine-homocysteine methyltransferase), Genebank Accession Number: AK055053 [SHMT2, Serine hydroxymethyltransferase 2 (mitochondrial)], Genebank Accession Number: NM—007101 (SARDH, Sarcosine dehydrogenase), Genebank Accession Number: AL833251 (LOC283970, Hypothetical protein LOC283970), Genebank Accession Number: AK027126 (ASS, Argininosuccinate synthetase), Genebank Accession Number: L00972 (CBS, Cystathionine-beta-synthase), Genebank Accession Number: NM—206965 (FTCD, Formiminotransferase cyclodeaminase), Genebank Accession Number: NM—000429 (MAT1A, Methionine adenosyltransferase I, alpha), Genebank Accession Number: NM—001801 (CDO1, Cysteine dioxygenase, type I), Genebank Accession Number: NM—006843 (SDS, Serine dehydratase), Genebank Accession Number: NM—000170 [GLDC, Glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavage system protein P)], Genebank Accession Number: BX102654 (HIST1H4B, Histone 1, H4b), Genebank Accession Number: DQ097177 (UREB1, HECT, UBA and WWE domain containing 1), Genebank Accession Number: NM—022743 (SMYD3, SET and MYND domain containing 3), Genebank Accession Number: NM—001809 (CENPA, Centromere protein A, 17 kDa), Genebank Accession Number: AB028450 (NCOR1, Nuclear receptor co-repressor 1), Genebank Accession Number: NM—002967 (SAFB, Scaffold attachment factor B), Genebank Accession Number: NM—003074 (SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1), Genebank Accession Number: NM—015409 (EP400, E1A binding protein p400), Genebank Accession Number: BC044659 (SMARCA3, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3), Genebank Accession Number: AB209433 (BAT8, Euchromatic histone-lysine N-methyltransferase 2), Genebank Accession Number: XM—370756 (KIAA1305, KIAA1305), Genebank Accession Number: NM—020201 (NT5M, 5′,3′-nucleotidase, mitochondrial), Genebank Accession Number: NM—001025248 (DUT, DUTP pyrophosphatase), Genebank Accession Number: AK123010 (RRM2, Ribonucleotide reductase M2 polypeptide), Genebank Accession Number: BX537961 (DNMT2, DNA (cytosine-5-)-methyltransferase 2), Genebank Accession Number: BX648935 (TBL1XR1, Transducin (beta)-like 1X-linked receptor 1), Genebank Accession Number: NM—001001888 (VCX-C, Variably charged X-C), Genebank Accession Number: NM—002501 [NFIX, Nuclear factor I/X (CCAAT-binding transcription factor)], Genebank Accession Number: NM—003483 (HMGA2, High mobility group AT-hook 2), Genebank Accession Number: U80628 (TK2, Thymidine kinase 2, mitochondrial), Genebank Accession Number: NM—006807 [CBX1, Chromobox homolog 1 (HP1 beta homolog Drosophila)], Genebank Accession Number: AY024361 (MLL3, Myeloid/lymphoid or mixed-lineage leukemia 3), Genebank Accession Number: NM—017519 [ARID1B, AT rich interactive domain 1B (SWI1-like)], Genebank Accession Number: NM—003077 (SMARCD2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2), Genebank Accession Number: NM—016937 (POLA, Polymerase (DNA directed), alpha), Genebank Accession Number: AJ007583 (LARGE, Like-glycosyltransferase), Genebank Accession Number: AK124635 (PCSK9, Proprotein convertase subtilisin/kexin type 9), Genebank Accession Number: NM—030758 (OSBP2, Oxysterol binding protein 2), Genebank Accession Number: NM—005063 [SCD, Stearoyl-CoA desaturase (delta-9-desaturase)], Genebank Accession Number: NM—000236 (LIPC, Lipase, hepatic), Genebank Accession Number: AF288389 (GLT25D2, Glycosyltransferase 25 domain containing 2), Genebank Accession Number: AF202889 (APOA5, Apolipoprotein A-V), Genebank Accession Number: BX640945 (FADS2, Fatty acid desaturase 2), Genebank Accession Number: BI521580 (APOC3, Apolipoprotein C-III), Genebank Accession Number: NM—001277 (CHKA, Choline kinase alpha), Genebank Accession Number: Z28339 [AKR1D1, Aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)], Genebank Accession Number: BC035654 (NR1H4, Nuclear receptor subfamily 1, group H, member 4), Genebank Accession Number: AB209229 (MVD, Mevalonate (diphospho) decarboxylase), Genebank Accession Number: BC026264 (UGT2B4, UDP glucuronosyltransferase 2 family, polypeptide B4), Genebank Accession Number: NM—004104 (FASN, Fatty acid synthase), Genebank Accession Number: NM—147161 (THEA, Acyl-CoA thioesterase 11), Genebank Accession Number: AF059203 (SOAT2, Sterol O-acyltransferase 2), Genebank Accession Number: NM—020918 (GPAM, Glycerol-3-phosphate acyltransferase, mitochondrial), Genebank Accession Number: NM—001443 (FABP1, Fatty acid binding protein 1, liver), Genebank Accession Number: NM—014762 (DHCR24, 24-dehydrocholesterol reductase), Genebank Accession Number: AK093328 (PCYT2, Phosphate cytidylyltransferase 2, ethanolamine), Genebank Accession Number: BX538214 (C6orf167, Chromosome 6 open reading frame 167), Genebank Accession Number: X75311 [MVK, Mevalonate kinase (mevalonic aciduria)], Genebank Accession Number: NM—000497 (CYP11B1, Cytochrome P450, family 11, subfamily B, polypeptide 1), Genebank Accession Number: BC036102 (SIAT7F, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6), Genebank Accession Number: BC012172 (ACAS2, Acyl-CoA synthetase short-chain family member 2), Genebank Accession Number: NM—007169 (PEMT, Phosphatidylethanolamine N-methyltransferase), Genebank Accession Number: X91148 (MTP, Microsomal triglyceride transfer protein), Genebank Accession Number: NM—198839 (ACACA, Acetyl-Coenzyme A carboxylase alpha), Genebank Accession Number: NM—004687 (MTMR4, Myotubularin related protein 4), Genebank Accession Number: NM—000384 [APOB, Apolipoprotein B (including Ag (x) antigen)], Genebank Accession Number: AK127051 [ACAA1, Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-oxoacyl-Coenzyme A thiolase)], Genebank Accession Number: BC035638 [LSS, Lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)], Genebank Accession Number: NM—004204 (PIGQ, Phosphatidylinositol glycan, class Q), Genebank Accession Number: NM—004457 (ACSL3, Acyl-CoA synthetase long-chain family member 3), Genebank Accession Number: AL138578 (ADPN, Adiponutrin), Genebank Accession Number: NM—002332 [LRP1, Low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)], Genebank Accession Number: NM—016372 (GPR175, G protein-coupled receptor 175), Genebank Accession Number: NM—080476 [CDC91L1, CDC91 cell division cycle 91-like 1 (S. cerevisiae)], Genebank Accession Number: AF303134 (ALDH8A1, Aldehyde dehydrogenase 8 family, member A1), Genebank Accession Number: NM—002130 [HMGCS1, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)], Genebank Accession Number: AK055414 [LASS4, LAG1 longevity assurance homolog 4 (S. cerevisiae)], Genebank Accession Number: NM—022977 (ACSL4, Acyl-CoA synthetase long-chain family member 4), Genebank Accession Number: NM—004631 (LRP8, Low density lipoprotein receptor-related protein 8, apolipoprotein e receptor), Genebank Accession Number: NM—002573 (PAFAH1B3, Platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29 kDa), Genebank Accession Number: NM—021005 (NR2F2, Nuclear receptor subfamily 2, group F, member 2), Genebank Accession Number: CR620135 [DPEP1, Dipeptidase 1 (renal)], Genebank Accession Number: NM—002317 (LOX, Lysyl oxidase), Genebank Accession Number: NM—005949 [MT1F, Metallothionein 1F (functional)], Genebank Accession Number: AF279145 (ANTXR1, Anthrax toxin receptor 1), Genebank Accession Number: NM—005588 [MEP1A, Meprin A, alpha (PABA peptide hydrolase)], Genebank Accession Number: NM—014214 (IMPA2, Inositol (myo)-1 (or 4)-monophosphatase 2), Genebank Accession Number: NM—005940 [MMP11, Matrix metallopeptidase 11 (stromelysin 3)], Genebank Accession Number: AB193259 (ACE2, Angiotensin I converting enzyme (peptidyl-dipeptidase A) 2), Genebank Accession Number: AF018081 (COL18A1, Collagen, type XVIII, alpha 1), Genebank Accession Number: M28016 (cytochrome b, Human mitochondrial cytochrome b gene, partial cds.), Genebank Accession Number: NM—002428 [MMP15, Matrix metallopeptidase 15 (membrane-inserted)], Genebank Accession Number: NM—025074 (FRAS1, Fraser syndrome 1), Genebank Accession Number: M75106 [CPB2, Carboxypeptidase B2 (plasma, carboxypeptidase U)], Genebank Accession Number: NM—000937 (POLR2A, Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa), Genebank Accession Number: NM—005060 (RORC, RAR-related orphan receptor C), Genebank Accession Number: BC092512 (MYRIP, Myosin VIIA and Rab interacting protein), Genebank Accession Number: NM—000901 (NR3C2, Nuclear receptor subfamily 3, group C, member 2), Genebank Accession Number: NM—005392 (PHF2, PHD finger protein 2), Genebank Accession Number: AK091289 (ZNF367, Zinc finger protein 367), Genebank Accession Number: NM—014788 (TRIM14, Tripartite motif-containing 14), Genebank Accession Number: NM—018660 (ZNF395, Zinc finger protein 395), Genebank Accession Number: AB209755 (MLLT10, Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10), Genebank Accession Number: AK097472 [MAZ, MYC-associated zinc finger protein (purine-binding transcription factor)], Genebank Accession Number: NM—020062 (SLC2A4RG, SLC2A4 regulator), Genebank Accession Number: BX571750 (HNF4G, Hepatocyte nuclear factor 4, gamma), Genebank Accession Number: NM—021807 (SEC8L1, Exocyst complex component 4), Genebank Accession Number: NM—006651 (CPLX1, Complexin 1), Genebank Accession Number: NM—005845 (ABCC4, ATP-binding cassette, sub-family C (CFTR/MRP), member 4), Genebank Accession Number: NM—006650 (CPLX2, Complexin 2), Genebank Accession Number: NM—004164 (RBP2, Retinol binding protein 2, cellular), Genebank Accession Number: NM—021069 (ARGBP2, Sorbin and SH3 domain containing 2), Genebank Accession Number: NM—201649 (SLC6A9, Solute carrier family 6 (neurotransmitter transporter, glycine), member 9), Genebank Accession Number: NM—005835 (SLC17A2, Solute carrier family 17 (sodium phosphate), member 2), Genebank Accession Number: AJ315644 (SLC2A13, Solute carrier family 2 (facilitated glucose transporter), member 13), Genebank Accession Number: NM—000340 (SLC2A2, Solute carrier family 2 (facilitated glucose transporter), member 2), Genebank Accession Number: NM—003685 [KHSRP, KH-type splicing regulatory protein (FUSE binding protein 2)], Genebank Accession Number: BX537382 (SLC38A3, Solute carrier family 38, member 3), Genebank Accession Number: NM—182964 (NAV2, Neuron navigator 2), Genebank Accession Number: BX640965 (SLC22A3, Solute carrier family 22 (extraneuronal monoamine transporter), member 3), Genebank Accession Number: AK127255 (SNX26, Sorting nexin 26), Genebank Accession Number: AY043484 (SCN1A, Sodium channel, voltage-gated, type I, alpha), Genebank Accession Number: AK074107 (C20orf59, Chromosome 20 open reading frame 59), Genebank Accession Number: NM—003056 (SLC19A1, Solute carrier family 19 (folate transporter), member 1), Genebank Accession Number: NM—020897 (HCN3, Hyperpolarization activated cyclic nucleotide-gated potassium channel 3), Genebank Accession Number: AY124771 (SLC26A1, Solute carrier family 26 (sulfate transporter), member 1), Genebank Accession Number: NM—001011554 (SLC13A3, Solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3), Genebank Accession Number: AK027855 (SLC5A6, Solute carrier family 5 (sodium-dependent vitamin transporter), member 6), Genebank Accession Number: NM—001005747 (CACNB4, Calcium channel, voltage-dependent, beta 4 subunit), Genebank Accession Number: NM—013277 (RACGAP1, Rac GTPase activating protein 1), Genebank Accession Number: NM—012340 (NFATC2, Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), Genebank Accession Number: NM—018291 (FLJ10986, Hypothetical protein FLJ10986), Genebank Accession Number: BC052951 (LMNB1, Lamin B1), Genebank Accession Number: AK075271 [SPTLC2L, Serine palmitoyltransferase, long chain base subunit 2-like (aminotransferase 2)], Genebank Accession Number: BI762502 (GSTA2, Glutathione S-transferase A2), Genebank Accession Number: AK055221 (FBXO5, F-box protein 5), Genebank Accession Number: XM—372695 (LOC390859, PREDICTED: Homo sapiens similar to Chain A, Crystal Structure Of The R463a Mutant Of Human Glutamate Dehydrogenase (LOC390859), mRNA), Genebank Accession Number: AB037805 [KLHL14, Kelch-like 14 (Drosophila)], Genebank Accession Number: NM—145061 (C13orf3, Chromosome 13 open reading frame 3), Genebank Accession Number: AK000643 (ASB9, Ankyrin repeat and SOCS box-containing 9), Genebank Accession Number: AL133031 (MLR1, Transcription factor MLR1), Genebank Accession Number: AB091343 (C10orf3, Centrosomal protein 55 kDa), Genebank Accession Number: AY007723 (MAL2, Mal, T-cell differentiation protein 2), Genebank Accession Number: BC098313 (FLJ20641, Hypothetical protein FLJ20641), Genebank Accession Number: BC041882 (ATF7IP2, Activating transcription factor 7 interacting protein 2), Genebank Accession Number: AK127098 (FLJ10706, Chromosome 1 open reading frame 112), Genebank Accession Number: BC022255 [Spc25, Spindle pole body component 25 homolog (S. cerevisiae)], and Genebank Accession Number: BC026283 [APOH, Apolipoprotein H (beta-2-glycoprotein I)].
1) The gene whose expression is increased by chrysene exposure is characteristically selected from the group consisting of the following genes:
Genebank Accession Number: NM—003954 (MAP3K14, Mitogen-activated protein kinase kinase kinase 14), Genebank Accession Number: AB209586 (MAPK13, Mitogen-activated protein kinase 13), Genebank Accession Number: NM—005343 (HRAS, V-Ha-ras Harvey rat sarcoma viral oncogene homolog), Genebank Accession Number: BX647104 (FOS, V-fos FBJ murine osteosarcoma viral oncogene homolog), Genebank Accession Number: NM—007315 (STAT1, Signal transducer and activator of transcription 1, 91 kDa), Genebank Accession Number: BC027933 (MAPK11, Mitogen-activated protein kinase 11), Genebank Accession Number: NM—020529 (NFKBIA, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), Genebank Accession Number: BC014966 (RPS6KA1, Ribosomal protein S6 kinase, 90 kDa, polypeptide 1), Genebank Accession Number: NM—181861 (APAF1, Apoptotic peptidase activating factor), Genebank Accession Number: L41870 [RB1, Retinoblastoma 1 (including osteosarcoma)], Genebank Accession Number: CR612719 (GADD45A, Growth arrest and DNA-damage-inducible, alpha), Genebank Accession Number: BM462208 (PCNA, Proliferating cell nuclear antigen), Genebank Accession Number: M92424 [MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)], Genebank Accession Number: NM—078467 [CDKN1A, Cyclin-dependent kinase inhibitor 1A (p21, Cip1)], Genebank Accession Number: BC089389 (IHPK3, Inositol hexaphosphate kinase 3), Genebank Accession Number: NM—001024807 (APLP1, Amyloid beta (A4) precursor-like protein 1), Genebank Accession Number: NM—001013398 (IGFBP3, Insulin-like growth factor binding protein 3), Genebank Accession Number: AB209361 [FAS, Fas (TNF receptor superfamily, member 6)], Genebank Accession Number: NM—147780 (CTSB, Cathepsin B), Genebank Accession Number: NM—001165 (BIRC3, Baculoviral IAP repeat-containing 3), Genebank Accession Number: BM557864 (HSPB1, Heat shock 27 kDa protein 1), Genebank Accession Number: AB033060 (PDCD6, Aryl-hydrocarbon receptor repressor), Genebank Accession Number: AK125880 (TP53INP1, Tumor protein p53 inducible nuclear protein 1), Genebank Accession Number: NM—004574 (SEP4, Septin 4), Genebank Accession Number: NM—003820 [TNFRSF14, Tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)], Genebank Accession Number: CN478604 [LGALS7, Lectin, galactoside-binding, soluble, 7 (galectin 7)], Genebank Accession Number: NM—004879 (EI24, Etoposide induced 2.4 mRNA), Genebank Accession Number: NM—003311 (PHLDA2, Pleckstrin homology-like domain, family A, member 2), Genebank Accession Number: NM—003897 (IER3, Immediate early response 3), Genebank Accession Number: AK097284 (TNFAIP8, Tumor necrosis factor, alpha-induced protein 8), Genebank Accession Number: NM—000582 [SPP1, Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)], Genebank Accession Number: NM—013258 (PYCARD, PYD and CARD domain containing), Genebank Accession Number: NM—002305 [LGALS1, Lectin, galactoside-binding, soluble, 1 (galectin 1)], Genebank Accession Number: AF055634 [UNC5C, Unc-5 homolog C (C. elegans)], Genebank Accession Number: CR614015 (CD14, CD14 antigen), Genebank Accession Number: AK129595 (GADD45B, Growth arrest and DNA-damage-inducible, beta), Genebank Accession Number: AF356193 (CARD6, Caspase recruitment domain family, member 6), Genebank Accession Number: BC063043 [PRF1, Perforin 1 (pore forming protein)], Genebank Accession Number: BX537586 [STK17A, Serine/threonine kinase 17a (apoptosis-inducing)], Genebank Accession Number: NM—003842 (TNFRSF10B, Tumor necrosis factor receptor superfamily, member 10b), Genebank Accession Number: AF208043 (IFI16, Interferon, gamma-inducible protein 16), Genebank Accession Number: BX641114 (ANXA4, Annexin A4), Genebank Accession Number: NM—001009552 (PPP2CB, Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform), Genebank Accession Number: BX386171 (CGB5, Chorionic gonadotropin, beta polypeptide 8), Genebank Accession Number: AY358103 (HIP1, Huntingtin interacting protein 1), Genebank Accession Number: BC027949 (P2RX1, Purinergic receptor P2X, ligand-gated ion channel, 1), Genebank Accession Number: BC042844 (CASP10, Caspase 10, apoptosis-related cysteine peptidase), Genebank Accession Number: NM—000733 [CD3E, CD3E antigen, epsilon polypeptide (TiT3 complex)], Genebank Accession Number: NM—033245 (PML, Promyelocytic leukemia), Genebank Accession Number: BC047362 (PHLDA1, Pleckstrin homology-like domain, family A, member 1), Genebank Accession Number: NM—006763 (BTG2, BTG family, member 2), Genebank Accession Number: BC050455 (DDB2, LIM homeobox 3), Genebank Accession Number: BC068535 (TP53AP1, TP53 activated protein 1), Genebank Accession Number: AF033122 (SESN1, Sestrin 1), Genebank Accession Number: AB024313 (POLH, Polymerase (DNA directed), eta), Genebank Accession Number: NM—001012716 (TYMS, Thymidylate synthetase), Genebank Accession Number: BC095402 (ATXN3, Ataxin 3), Genebank Accession Number: BU675073 (PTTG1, Pituitary tumor-transforming 1), Genebank Accession Number: BC073161 (RAD51C, RAD51 homolog C (S. cerevisiae), Genebank Accession Number: NM—001983 [ERCC1, Excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)], Genebank Accession Number: NM—018965 (TREM2, Triggering receptor expressed on myeloid cells 2), Genebank Accession Number: BX649164 (PAI, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1), Genebank Accession Number: NM—006404 [PROCR, Protein C receptor, endothelial (EPCR)], Genebank Accession Number: C035582 (TRIM22, Tripartite motif-containing 22), Genebank Accession Number: NM—006691 (XLKD1, Extracellular link domain containing 1), Genebank Accession Number: NM—015831 [ACHE, Acetylcholinesterase (YT blood group)], Genebank Accession Number: BC045666 (TP53I11, Tumor protein p53 inducible protein 11), Genebank Accession Number: NM—005438 (FOSL1, FOS-like antigen 1), Genebank Accession Number: NM—005755 (EBI3, Epstein-Barr virus induced gene 3), Genebank Accession Number: BM918324 (LY96, Lymphocyte antigen 96), Genebank Accession Number: NM—000022 (ADA, Adenosine deaminase), Genebank Accession Number: NM—004167 (CCL14, Chemokine (C-C motif) ligand 15), Genebank Accession Number: NM—014278 (HSPA4L, Heat shock 70 kDa protein 4-like), Genebank Accession Number: BC006523 (SGK2, Serum/glucocorticoid regulated kinase 2), Genebank Accession Number: NM—000641 (IL11, Interleukin 11), Genebank Accession Number: H18681 [C20orf139, Sulfiredoxin 1 homolog (S. cerevisiae)], Genebank Accession Number: NM—001769 [CD9, CD9 antigen (p24)], Genebank Accession Number: BG564326 (SAA4, Serum amyloid A4, constitutive), Genebank Accession Number: NM—016816 (OAS1, 2′,5′-oligoadenylate synthetase 1, 40/46 kDa), Genebank Accession Number: CB055213 (ICOSLG, Inducible T-cell co-stimulator ligand), Genebank Accession Number: CR591007 (CCL3, Chemokine (C-C motif) ligand 3), Genebank Accession Number: NM—145641 (APOL3, Apolipoprotein L, 3), Genebank Accession Number: AK127663 (PTGES, Prostaglandin E synthase), Genebank Accession Number: NM—001734 (C1S, Complement component 1, s subcomponent), Genebank Accession Number: NM—002260 (KLRC3, Killer cell lectin-like receptor subfamily C, member 2), Genebank Accession Number: NM—001733 (C1R, Complement component 1, r subcomponent), Genebank Accession Number: AB209922 (DNAJB4, DnaJ (Hsp40) homolog, subfamily B, member 4), Genebank Accession Number: NM—201397 (GPX1, Glutathione peroxidase 1), Genebank Accession Number: AK090482 [CXCL12, Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)], Genebank Accession Number: BF662985 (CCL3L1, Chemokine (C-C motif) ligand 3-like 1), Genebank Accession Number: NM—001779 [CD58, CD58 antigen, (lymphocyte function-associated antigen 3)], Genebank Accession Number: NM—005064 (CCL23, Chemokine (C-C motif) ligand 23), Genebank Accession Number: NM—000242 [MBL2, Mannose-binding lectin (protein C) 2, soluble (opsonic defect)], Genebank Accession Number: NM—032036 (FAM14A, Family with sequence similarity 14, member A), Genebank Accession Number: AJ890082 (HSPCA, Heat shock 90 kDa protein 1, alpha), Genebank Accession Number: BC068487 (TLR3, Toll-like receptor 3), Genebank Accession Number: NM—000552 (VWF, Von Willebrand factor), Genebank Accession Number: NM—000132 [F8, Coagulation factor VIII, procoagulant component (hemophilia A)], Genebank Accession Number: AY154461 (NALP6, NACHT, leucine rich repeat and PYD containing 6), Genebank Accession Number: AK127679 (DUSP1, Dual specificity phosphatase 1), Genebank Accession Number: NM—014314 (DDX58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58), Genebank Accession Number: NM—000311 [PRNP, Prion protein (p27-30) (Creutzfeld-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)], Genebank Accession Number: NM—006597 (HSPA8, Heat shock 70 kDa protein 8), Genebank Accession Number: AK092040 (ZCSL2, Zinc finger, CSL-type containing 2), Genebank Accession Number: NM—031483 [ITCH, Itchy homolog E3 ubiquitin protein ligase (mouse)], Genebank Accession Number: U25029 [NR3C1, Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)], Genebank Accession Number: NM—005242 (F2RL1, Coagulation factor II (thrombin) receptor-like 1), Genebank Accession Number: AK172851 (ADORA2B, Adenosine A2b receptor), Genebank Accession Number: BQ053419 (BATF, Basic leucine zipper transcription factor, ATF-like), Genebank Accession Number: BC020698 (CCL20, Chemokine (C-C motif) ligand 20), Genebank Accession Number: NM—001124 (ADM, Adrenomedullin), Genebank Accession Number: NM—153609 (TMPRSS6, Transmembrane protease, serine 6), Genebank Accession Number: NM—004148 (NINJ1, Ninjurin 1), Genebank Accession Number: AF324224 (APOL2, Apolipoprotein L, 2), Genebank Accession Number: BC040494 (DNAJB2, DnaJ (Hsp40) homolog, subfamily B, member 2), Genebank Accession Number: BE512691 [GPX2, Glutathione peroxidase 2 (gastrointestinal)], Genebank Accession Number: NM—001002029 (C4A, Complement component 4B, telomeric), Genebank Accession Number: NM—000732 [CD3D, CD3D antigen, delta polypeptide (TiT3 complex)], Genebank Accession Number: AK223210 [CD79B, CD79B antigen (immunoglobulin-associated beta)], Genebank Accession Number: NM—002309 (LIF, Hypothetical protein MGC20647), Genebank Accession Number: AF031469 (MR1, Major histocompatibility complex, class I-related), Genebank Accession Number: NM—172208 [TAPBP, TAP binding protein (tapasin)], Genebank Accession Number: BC021117 [CSF1, Colony stimulating factor 1 (macrophage)], Genebank Accession Number: NM—001421 [ELF4, E74-like factor 4 (ets domain transcription factor)], Genebank Accession Number: NM—002119 (HLA-DOA, Major histocompatibility complex, class II, DO alpha), Genebank Accession Number: BX648013 [TAP1, Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)], Genebank Accession Number: AL832287 (ZAP70, Zeta-chain (TCR) associated protein kinase 70 kDa), Genebank Accession Number: BF569086 (NK4, Interleukin 32), Genebank Accession Number: AL832451 (DKFZp451C2311, guanylate binding protein 2, interferon-inducible), Genebank Accession Number: AF069493 (SEMA7A, Sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A), Genebank Accession Number: NM—001008490 (KLF6, Kruppel-like factor 6), Genebank Accession Number: NM—000418 (IL4R, Interleukin 4 receptor), Genebank Accession Number: NM—004048 (B2M, Beta-2-microglobulin), Genebank Accession Number: AF116720 (DNAJA2, DnaJ (Hsp40) homolog, subfamily A, member 2), Genebank Accession Number: NM—001007271 (DUSP13, Dual specificity phosphatase 13), Genebank Accession Number: BM904612 [S100A6, S100 calcium binding protein A6 (calcyclin)], Genebank Accession Number: AK074652 (SERTAD1, SERTA domain containing 1), Genebank Accession Number: NM—203394 (E2F7, E2F transcription factor 7), Genebank Accession Number: NM—003620 (PPM1D, Protein phosphatase 1D magnesium-dependent, delta isoform), Genebank Accession Number: BC060797 [PARD6G, Par-6 partitioning defective 6 homolog gamma (C. elegans)], Genebank Accession Number: NM—021913 (AXL, AXL receptor tyrosine kinase), Genebank Accession Number: BC040303 (PTP4A1, Protein tyrosine phosphatase type IVA, member 1), Genebank Accession Number: BC008564 (PPP1R9B, Protein phosphatase 1, regulatory subunit 9B, spinophilin), Genebank Accession Number: AK097024 [RAE1, RAE1 RNA export 1 homolog (S. pombe)], Genebank Accession Number: BC011957 (BTG3, BTG family, member 3), Genebank Accession Number: NM—078487 [CDKN2B, Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)], Genebank Accession Number: BC023552 (SFN, Stratifin), Genebank Accession Number: BC060847 [PARD6B, Par-6 partitioning defective 6 homolog beta (C. elegans)], Genebank Accession Number: NM—003236 (TGFA, Transforming growth factor, alpha), Genebank Accession Number: NM—002632 (PGF, Placental growth factor, vascular endothelial growth factor-related protein), Genebank Accession Number: NM—015278 (SASH1, SAM and SH3 domain containing 1), Genebank Accession Number: AL834276 (MGC3207, Hypothetical protein MGC3207), Genebank Accession Number: AK096276 (CCND3, Cyclin D3), Genebank Accession Number: AB209373 (CCNK, Chromosome 14 open reading frame 65), Genebank Accession Number: NM—021132 [PPP3CB, Protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)], Genebank Accession Number: NM—005072 (SLC12A4, Solute carrier family 12 (potassium/chloride transporters), member 4), Genebank Accession Number: BX647886 [PCNP, SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae)], Genebank Accession Number: NM—044472 [CDC42, Cell division cycle 42 (GTP binding protein, 25 kDa)], Genebank Accession Number: AL832973 [REC8L1, REC8-like 1 (yeast)], Genebank Accession Number: NM—002031 (FRK, Fyn-related kinase), Genebank Accession Number: BM913048 (TIMP1, TIMP metallopeptidase inhibitor 1), Genebank Accession Number: NM—003975 (SH2D2A, SH2 domain protein 2A), Genebank Accession Number: NM—198129 (LAMA3, Laminin, alpha 3), Genebank Accession Number: NM—006152 (LRMP, Lymphoid-restricted membrane protein), Genebank Accession Number: X96753 [CSPG4, Chondroitin sulfate proteoglycan 4 (melanoma-associated)], Genebank Accession Number: NM—000474 [TWIST1, Twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)], Genebank Accession Number: AF052152 (DCAMKL1, Doublecortin and CaM kinase-like 1), Genebank Accession Number: AF333334 (CCIN, Calicin), Genebank Accession Number: NM—005979 (S100A13, S100 calcium binding protein A13), Genebank Accession Number: NM—004429 (EFNB1, Ephrin-B1), Genebank Accession Number: NM—003873 (NRP1, Neuropilin 1), Genebank Accession Number: AF026692 (SFRP4, Secreted frizzled-related protein 4), Genebank Accession Number: AK124583 (DAZAP1, DAZ associated protein 1), Genebank Accession Number: AF003837 [JAG1, Jagged 1 (Alagille syndrome)], Genebank Accession Number: AB209590 (BMP1, Bone morphogenetic protein 1), Genebank Accession Number: X54457 [CEL, Carboxyl ester lipase (bile salt-stimulated lipase)], Genebank Accession Number: Y11307 (CYR61, Cysteine-rich, angiogenic inducer, 61), Genebank Accession Number: NM—000596 (IGFBP1, Insulin-like growth factor binding protein 1), Genebank Accession Number: NM—020997 (LEFTY1, Left-right determination factor 1), Genebank Accession Number: NM—001553 (IGFBP7, Insulin-like growth factor binding protein 7), Genebank Accession Number: AB209509 (IGFBP2, Insulin-like growth factor binding protein 2, 36 kDa), Genebank Accession Number: NM—001552 (IGFBP4, Insulin-like growth factor binding protein 4), Genebank Accession Number: AB209321 (CSRP2, Cysteine and glycine-rich protein 2), Genebank Accession Number: BM556279 (EMP3, Epithelial membrane protein 3), Genebank Accession Number: NM—005727 (TSPAN1, Tetraspanin 1), Genebank Accession Number: BQ683841 [S100A11, S100 calcium binding protein A11 (calgizzarin)], Genebank Accession Number: BC042390 [VTI1B, Vesicle transport through interaction with t-SNAREs homolog 1B (yeast)], Genebank Accession Number: NM—000899 (KITLG, KIT ligand), Genebank Accession Number: BC032940 (LAMP3, Lysosomal-associated membrane protein 3), Genebank Accession Number: BQ939577 [AKR1C3, Aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)], Genebank Accession Number: BC063468 (OSMR, Oncostatin M receptor), Genebank Accession Number: CR936719 [SSR1, Signal sequence receptor, alpha (translocon-associated protein alpha)], Genebank Accession Number: AB024057 [TBC1D8, TBC1 domain family, member 8 (with GRAM domain)], Genebank Accession Number: NM—003329 (TXN, Thioredoxin), Genebank Accession Number: NM—012125 (CHRM5, Cholinergic receptor, muscarinic 5), Genebank Accession Number: NM—007045 (FGFR1OP, FGFR1 oncogene partner), Genebank Accession Number: AK094474 (RHOC, Ras homolog gene family, member C), Genebank Accession Number: BC017706 (6PGL, 6-phosphogluconolactonase), Genebank Accession Number: AF153821 (ADH1B, Alcohol dehydrogenase IB (class I), beta polypeptide), Genebank Accession Number: AK124656 [ENO2, Enolase 2 (gamma, neuronal)], Genebank Accession Number: D42047 (GPD1L, Glycerol-3-phosphate dehydrogenase 1-like), Genebank Accession Number: AK130060 (TALDO1, Transaldolase 1), Genebank Accession Number: AL833069 (KIAA1434, Hypothetical protein KIAA1434), Genebank Accession Number: XM-496436 (UNQ6077, C219-reactive peptide), Genebank Accession Number: NM—199186 (BPGM, 2,3-bisphosphoglycerate mutase), Genebank Accession Number: U86136 (TEP1, Telomerase-associated protein 1), Genebank Accession Number: BU603483 (HIST1H1E, Histone 1, H1e), Genebank Accession Number: NM—005319 (HIST1H1C, Histone 1, H1c), Genebank Accession Number: NM—005320 (HIST1H1D, Histone 1, H1d), Genebank Accession Number: NM—003516 (HIST2H2AA, Histone 2, H2aa), Genebank Accession Number: F02250 (HDAC2, Histone deacetylase 2), Genebank Accession Number: NM—004739 (MTA2, Metastasis associated 1 family, member 2), Genebank Accession Number: BC042589 (HIST1H2BD, Histone 1, H2bd), Genebank Accession Number: CB529694 (HIST2H2BE, Histone 2, H2be), Genebank Accession Number: AK126154 (NP, Nucleoside phosphorylase), Genebank Accession Number: AB040899 (KIAA1466, KIAA1466 gene), Genebank Accession Number: CR608156 (HIST1H2AC, Histone 1, H2ac), Genebank Accession Number: BX436525 (HIST1H2BH, Histone 1, H2bh), Genebank Accession Number: AK057762 (TDH, L-threonine dehydrogenase), Genebank Accession Number: BC035124 (UNQ2541, MSFL2541), Genebank Accession Number: BC034763 (FDXR, Ferredoxin reductase), Genebank Accession Number: AK093461 (ISYNA1, Myo-inositol 1-phosphate synthase A1), Genebank Accession Number: AK097009 (PLTP, phospholipid transfer protein), Genebank Accession Number: AF524864 [AKR1B10, Aldo-keto reductase family 1, member B10 (aldose reductase)], Genebank Accession Number: NM—021727 (FADS3, Fatty acid desaturase 3), Genebank Accession Number: CR627415 (HSD3B2, Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2), Genebank Accession Number: CD013987 (CYP21A2, Cytochrome P450, family 21, subfamily A, polypeptide 2), Genebank Accession Number: BC020744 (AKR1C4, Aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol), dehydrogenase 4), Genebank Accession Number: NM—004753 (DHRS3, Dehydrogenase/reductase (SDR family) member 3), Genebank Accession Number: BC030207 (NR0B2, Nuclear receptor subfamily 0, group B, member 2), Genebank Accession Number: AK091948 (LTB4DH, Leukotriene B4 12-hydroxydehydrogenase), Genebank Accession Number: NM—002900 (RBP3, Retinol binding protein 3, interstitial), Genebank Accession Number: BX647927 (PLCD1, Phospholipase C, delta 1), Genebank Accession Number: NM—145323 (OSBPL3, Oxysterol binding protein-like 3), Genebank Accession Number: NM—000169 (GLA, Galactosidase, alpha), Genebank Accession Number: BC094756 (CRA, Myotubularin related protein 11), Genebank Accession Number: AB043587 (ACBD3, Acyl-Coenzyme A binding domain containing 3), Genebank Accession Number: NM—001478 [GALGT, UDP-N-acetyl-alpha-D-galactosamine: (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase (GalNAc-T)], Genebank Accession Number: J05428 (UGT2B7, UDP glucuronosyltransferase 2 family, polypeptide B7), Genebank Accession Number: AB002454 (CYP4F3, Cytochrome P450, family 4, subfamily F, polypeptide 3), Genebank Accession Number: NM—000405 (GM2A, GM2 ganglioside activator), Genebank Accession Number: BC001823 (PRKAB1, Protein kinase, AMP-activated, beta 1 non-catalytic subunit), Genebank Accession Number: NM—145343 (APOL1, Apolipoprotein L, 1), Genebank Accession Number: NM—006571 (DCTN6, Dynactin 6), Genebank Accession Number: NM—020139 (DHRS6, Dehydrogenase/reductase (SDR family) member 6), Genebank Accession Number: NM—000499 (CYP1A1, Cytochrome P450, family 1, subfamily A, polypeptide 1), Genebank Accession Number: BC007003 [TGM4, Transglutaminase 4 (prostate)], Genebank Accession Number: NM—012189 [CABYR, Calcium binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2)], Genebank Accession Number: CR615530 (CYGB, Cytoglobin), Genebank Accession Number: NM—004199 (P4HA2, Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II), Genebank Accession Number: AK027560 (CYP26A1, Cytochrome P450, family 26, subfamily A, polypeptide 1), Genebank Accession Number: NM—145298 (APOBEC3F, Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F), Genebank Accession Number: NM—005576 (LOXL1, Lysyl oxidase-like 1), Genebank Accession Number: AK024854 [APOBEC3B, Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D (putative)], Genebank Accession Number: NM—014301 (NIFUN, NifU-like N-terminal domain containing), Genebank Accession Number: BF670653 (MB, Myoglobin), Genebank Accession Number: AK126982 (LHX6, LIM homeobox 6), Genebank Accession Number: Y13786 [ADAM19, ADAM metallopeptidase domain 19 (meltrin beta)], Genebank Accession Number: AB051455 (MICAL-L1, MICAL-like 1), Genebank Accession Number: AK074068 (MICAL-L2, MICAL-like 2), Genebank Accession Number: AK124421 [CPA1, Carboxypeptidase A1 (pancreatic)], Genebank Accession Number: NM—030622 (CYP2S1, Cytochrome P450, family 2, subfamily S, polypeptide 1), Genebank Accession Number: BC012027 (CYP2U1, Cytochrome P450, family 2, subfamily U, polypeptide 1), Genebank Accession Number: AY461717 (ZNF385, Zinc finger protein 385), Genebank Accession Number: AK092424 (ZSCAN4, Zinc finger and SCAN domain containing 4), Genebank Accession Number: AB208935 [RASAL1, RAS protein activator like 1 (GAP1 like)], Genebank Accession Number: NM—201444 (DGKA, Diacylglycerol kinase, alpha 80 kDa), Genebank Accession Number: BC035625 [EGR2, Early growth response 2 (Krox-20 homolog, Drosophila)], Genebank Accession Number: BF983391 (RPS27L, Ribosomal protein S27-like), Genebank Accession Number: BC036545 (PLAC2, Placenta-specific 2), Genebank Accession Number: BC034569 (ZNF337, Zinc finger protein 337), Genebank Accession Number: CR936707 (TRIM38, Tripartite motif-containing 38), Genebank Accession Number: AK091940 (MGC4734, Ring finger protein 183), Genebank Accession Number: NM—024101 (MLPH, Melanophilin), Genebank Accession Number: CR601823 (HIVEP3, Human immunodeficiency virus type I enhancer binding protein 3), Genebank Accession Number: BC041578 (MGC17986, zinc finger protein 114), Genebank Accession Number: BC038432 (RARA, Retinoic acid receptor, alpha), Genebank Accession Number: BX641053 (FXYD2, FXYD domain containing ion transport regulator 2), Genebank Accession Number: AK095363 (AQP3, Aquaporin 3), Genebank Accession Number: AK074100 (SLC37A2, Solute carrier family 37 (glycerol-3-phosphate transporter), member 2), Genebank Accession Number: AK024002 (TUBA4, Tubulin, alpha 4), Genebank Accession Number: AK092677 (TUBB6, Tubulin, beta 6), Genebank Accession Number: NM—001297 (CNGB1, Cyclic nucleotide gated channel beta 1), Genebank Accession Number: NM—032872 (SYTL1, Synaptotagmin-like 1), Genebank Accession Number: NM—014045 (LRP10, Low density lipoprotein receptor-related protein 10), Genebank Accession Number: NM—014585 (SLC40A1, Solute carrier family 40 (iron-regulated transporter), member 1), Genebank Accession Number: L02870 [COL7A1, Collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive)], Genebank Accession Number: AK054731 [TUBA1, Tubulin, alpha 1 (testis specific)], Genebank Accession Number: AF336127 (SLC4A11, Solute carrier family 4, sodium bicarbonate transporter-like, member 11), Genebank Accession Number: AB075951 (SLC22A2, Solute carrier family 22 (organic cation transporter), member 2), Genebank Accession Number: AK125026 (SLC6A12, Solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12), Genebank Accession Number: BX537541 (MGC13186, Chromosome 1 open reading frame 57), Genebank Accession Number: CD013956 (SHBG, Sex hormone-binding globulin), Genebank Accession Number: BC029057 (DAO, D-amino-acid oxidase), Genebank Accession Number: BC041479 (COL9A2, Collagen, type IX, alpha 2), Genebank Accession Number: AL050210 (LOC283130, Hypothetical protein LOC283130), Genebank Accession Number: NM—004798 (KIF3B, Kinesin family member 3B), Genebank Accession Number: AK025742 [UCP2, Uncoupling protein 2 (mitochondrial, proton carrier)], Genebank Accession Number: NM—021990 (GABRE, Gamma-aminobutyric acid (GABA) A receptor, epsilon), Genebank Accession Number: NM—005819 (STX6, Syntaxin 6), Genebank Accession Number: BC092420 [CYP4F11, Similar to Cytochrome P450 4F12 (CYPIVF12) (UNQ568/PRO1129)], Genebank Accession Number: BC043423 (ABCB6, ATP-binding cassette, sub-family B (MDR/TAP), member 6), Genebank Accession Number: BC021025 (CTHRC1, Collagen triple helix repeat containing 1), Genebank Accession Number: NM—004999 (MYO6, Myosin VI), Genebank Accession Number: AF445025 (SLC35A4, Solute carrier family 35, member A4), Genebank Accession Number: NM—003330 (TXNRD1, Thioredoxin reductase 1), Genebank Accession Number: AK092512 (SLC16A5, Solute carrier family 16 (monocarboxylic acid transporters), member 5), Genebank Accession Number: AB075871 [FLJ42654, Golgi transport 1 homolog A (S. cerevisiae)], Genebank Accession Number: NM—004864 (GDF15, Growth differentiation factor 15), Genebank Accession Number: NM—022153 (PP2135, Chromosome 10 open reading frame 54), Genebank Accession Number: NM—001013632 (LOC343521, Similar to hypothetical protein 4833401D15), Genebank Accession Number: AK124751 (CAPN2, Calpain 2, (m/II) large subunit), Genebank Accession Number: AK095079 (RGC32, Response gene to complement 32), Genebank Accession Number: NM—031286 (SH3BGRL3, SH3 domain binding glutamic acid-rich protein like 3), Genebank Accession Number: AK126182 (KIAA1949, KIAA1949), Genebank Accession Number: NM—080388 (S100A16, S100 calcium binding protein A16), Genebank Accession Number: NM—025106 (SSB1, SplA/ryanodine receptor domain and SOCS box containing 1), Genebank Accession Number: NM—203403 (C9orf150, Chromosome 9 open reading frame 150), Genebank Accession Number: AK096241 (SQSTM1, Sequestosome 1), Genebank Accession Number: BQ276852 (TMSB10, Thymosin, beta 10), Genebank Accession Number: BC101655 (HIST1H2BI, Histone 1, H2bi), Genebank Accession Number: W84524 (TM4SF5, Transmembrane 4 L six family member 5), and Genebank Accession Number: BX647290 (HIST1H2BO, Histone 1, H2bo).
2) The gene whose expression is decreased by chrysene exposure is characteristically selected from the group consisting of the following genes:
Genebank Accession Number: NM—002467 [MYC, V-myc myelocytomatosis viral oncogene homolog (avian)], Genebank Accession Number: BC038295 (MAP2K7, Mitogen-activated protein kinase kinase 7), Genebank Accession Number: NM—004364 (CEBPA, CCAAT/enhancer binding protein (C/EBP), alpha), Genebank Accession Number: NM—145161 (MAP2K5, Mitogen-activated protein kinase kinase 5), Genebank Accession Number: XM—042066 (MAP3K1, Mitogen-activated protein kinase kinase kinase 1), Genebank Accession Number: Z25424 (MKNK2, MAP kinase interacting serine/threonine kinase 2), Genebank Accession Number: AB209047 (ELK1, ELK1, member of ETS oncogene family), Genebank Accession Number: NM—004755 (RPS6KA5, Ribosomal protein S6 kinase, 90 kDa, polypeptide 5), Genebank Accession Number: AB001872 (RPL4, Mitogen-activated protein kinase kinase kinase 13), Genebank Accession Number: NM—002758 (MAP2K6, Mitogen-activated protein kinase kinase 6), Genebank Accession Number: AK098095 (BRAF, V-raf murine sarcoma viral oncogene homolog B1), Genebank Accession Number: AB208919 [FGFR1, Fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)], Genebank Accession Number: BC060837 (DUSP9, Dual specificity phosphatase 9), Genebank Accession Number: NM—000142 [FGFR3, Fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism)], Genebank Accession Number: AY247738 [TRIB3, Tribbles homolog 3 (Drosophila)], Genebank Accession Number: NM—030640 (DUSP16, Dual specificity phosphatase 16), Genebank Accession Number: NM—148957 (TNFRSF19, Tumor necrosis factor receptor superfamily, member 19), Genebank Accession Number: AB011123 (TNIK, TRAF2 and NCK interacting kinase), Genebank Accession Number: NM—006549 (CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, beta), Genebank Accession Number: NM—022740 (HIPK2, Homeodomain interacting protein kinase 2), Genebank Accession Number: NM—005400 (PRKCE, Protein kinase C, epsilon), Genebank Accession Number: NM—000893 (KNG1, Kininogen 1), Genebank Accession Number: NM—000066 (C8B, Complement component 8, beta polypeptide), Genebank Accession Number: CR749293 (PLG, Plasminogen), Genebank Accession Number: BC013780 (ADORA2A, Adenosine A2a receptor), Genebank Accession Number: NM—001012271 (BIRC5, Effector cell peptidase receptor 1), Genebank Accession Number: NM—003811 (TNFSF9, Tumor necrosis factor (ligand) superfamily, member 9), Genebank Accession Number: BC050369 (E2F1, E2F transcription factor 1), Genebank Accession Number: NM—007313 (ABL1, V-abl Abelson murine leukemia viral oncogene homolog 1), Genebank Accession Number: BX647151 (MYBL2, V-myb myeloblastosis viral oncogene homolog (avian)-like 2), Genebank Accession Number: NM—004822 (NTN1, Netrin 1), Genebank Accession Number: BC033694 [BCL2L11, BCL2-like 11 (apoptosis facilitator)], Genebank Accession Number: AB208876 (AXIN1, Axin 1), Genebank Accession Number: BC051332 [F2, Coagulation factor II (thrombin)], Genebank Accession Number: AK122614 (PPARD, Peroxisome proliferative activated receptor, delta), Genebank Accession Number: L78790 [ITGB2, Integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)], Genebank Accession Number: AK223409 (BARD1, BRCA1 associated RING domain 1), Genebank Accession Number: NM—002737 (PRKCA, Protein kinase C, alpha), Genebank Accession Number: NM—012479 (YWHAG, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide), Genebank Accession Number: CR596268 [ITGB3BP, Integrin beta 3 binding protein (beta 3-endonexin)], Genebank Accession Number: NM—003551 [NME5, Non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)], Genebank Accession Number: NM—013974 (DDAH2, Dimethylarginine dimethylaminohydrolase 2), Genebank Accession Number: D79987 [ESPL1, Extra spindle poles like 1 (S. cerevisiae)], Genebank Accession Number: NM—006427 (SIVA, CD27-binding (Siva) protein), Genebank Accession Number: NM—024900 (PHF17, PHD finger protein 17), Genebank Accession Number: AB046854 (MAGI-3, Membrane associated guanylate kinase, WW and PDZ domain containing 3), Genebank Accession Number: AK095578 (SPHK1, Sphingosine kinase 1), Genebank Accession Number: AK122762 (CIDEA, Cell death-inducing DFFA-like effector a), Genebank Accession Number: AK092872 [ERCC2, Excision repair cross-complementing rodent repair deficiency, complementation group 2 (xeroderma pigmentosum D)], Genebank Accession Number: NM—006290 (TNFAIP3, Tumor necrosis factor, alpha-induced protein 3), Genebank Accession Number: NM—000562 (C8A, Complement component 8, alpha polypeptide), Genebank Accession Number: AK122894 (AKT1, V-akt murine thymoma viral oncogene homolog 1), Genebank Accession Number: NM—005067 [SIAH2, Seven in absentia homolog 2 (Drosophila)], Genebank Accession Number: NM—203339 [CLU, Clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)], Genebank Accession Number: NM—003376 (VEGF, Vascular endothelial growth factor), Genebank Accession Number: AK027590 (DAPK3, Death-associated protein kinase 3), Genebank Accession Number: AK122825 (HMGB1, High-mobility group box 1), Genebank Accession Number: NM—006904 (PRKDC, Protein kinase, DNA-activated, catalytic polypeptide), Genebank Accession Number: AK074377 (UHRF1, Chromosome 19 open reading frame 31), Genebank Accession Number: CR604810 (CCNA2, Cyclin A2), Genebank Accession Number: AK001720 [FLJ10858, Nei endonuclease VIII-like 3 (E. coli)], Genebank Accession Number: CR600021 (HMGB2, High-mobility group box 2), Genebank Accession Number: NM—006230 (POLD2, Polymerase (DNA directed), delta 2, regulatory subunit 50 kDa), Genebank Accession Number: NM—018151 [RIF1, RAP1 interacting factor homolog (yeast)], Genebank Accession Number: BX248766 [RAD51L1, RAD51-like 1 (S. cerevisiae)], Genebank Accession Number: NM—014502 [PRP19, PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)], Genebank Accession Number: NM—013975 (LIG3, Ligase III, DNA, ATP-dependent), Genebank Accession Number: NM—173627 (FLJ35220, Hypothetical protein FLJ35220), Genebank Accession Number: NM—003362 (UNG, Uracil-DNA glycosylase), Genebank Accession Number: AF076494 (IRF7, Interferon regulatory factor 7), Genebank Accession Number: CR625391 (RAD51AP1, RAD51 associated protein 1), Genebank Accession Number: NM—006231 (POLE, Polymerase (DNA directed), epsilon), Genebank Accession Number: NM—130398 (EXO1, Exonuclease 1), Genebank Accession Number: DQ140356 (KIAA1596, Fanconi anemia, complementation group M), Genebank Accession Number: NM—002105 (H2AFX, H2A histone family, member X), Genebank Accession Number: AB209560 (POLD1, Polymerase (DNA directed), delta 1, catalytic subunit 125 kDa), Genebank Accession Number: NM—000136 (FANCC, Fanconi anemia, complementation group C), Genebank Accession Number: NM—003368 (USP1, Ubiquitin specific peptidase 1), Genebank Accession Number: NM—022836 [DCLRE1B, DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)], Genebank Accession Number: NM—001067 (TOP2A, Topoisomerase (DNA) II alpha 170 kDa), Genebank Accession Number: NM—002913 (RFC1, Replication factor C (activator 1) 1, 145 kDa), Genebank Accession Number: NM—002412 (MGMT, O-6-methylguanine-DNA methyltransferase), Genebank Accession Number: NM—000251 [MSH2, MutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)], Genebank Accession Number: NM—015254 (KIF13B, Kinesin family member 13B), Genebank Accession Number: NM—006037 (HDAC4, Histone deacetylase 4), Genebank Accession Number: NM—020995 (HPR, Haptoglobin-related protein), Genebank Accession Number: NM—153443 (KIR3DL1, Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1), Genebank Accession Number: BC071718 (CCL4L1, Chemokine (C-C motif) ligand 4-like 1), Genebank Accession Number: NM—020979 (APS, Adaptor protein with pleckstrin homology and src homology 2 domains), Genebank Accession Number: NM—001629 (ALOX5AP, Arachidonate 5-lipoxygenase-activating protein), Genebank Accession Number: NM—130852 (PLUNC, Palate, lung and nasal epithelium carcinoma associated), Genebank Accession Number: AK024499 (SPON2, Spondin 2, extracellular matrix protein), Genebank Accession Number: BC048198 (AHSG, Alpha-2-HS-glycoprotein), Genebank Accession Number: AK095849 (RNU22, RNA, U22 small nucleolar), Genebank Accession Number: BC005395 (HPX, Hemopexin), Genebank Accession Number: NM—012276 (ILT7, Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4), Genebank Accession Number: NM—003152 (STAT5A, Signal transducer and activator of transcription 5A), Genebank Accession Number: BC037171 (CCL27, Chemokine (C-C motif) ligand 27), Genebank Accession Number: NM—002468 [MYD88, Myeloid differentiation primary response gene (88)], Genebank Accession Number: NM—002215 (ITIH1, Inter-alpha (globulin) inhibitor H1), Genebank Accession Number: NM—004750 (CRLF1, Cytokine receptor-like factor 1), Genebank Accession Number: DR004094 (HAMP, Hepcidin antimicrobial peptide), Genebank Accession Number: BC063245 (GNLY, Granulysin), Genebank Accession Number: AJ290445 (SARM1, Sterile alpha and TIR motif containing 1), Genebank Accession Number: AK122686 [IF, I factor (complement)], Genebank Accession Number: AB008535 (GPR44, G protein-coupled receptor 44), Genebank Accession Number: AK122912 (ZGPAT, Zinc finger, CCCH-type with G patch domain), Genebank Accession Number: BC022312 (C4BPA, Complement component 4 binding protein, alpha), Genebank Accession Number: BG564683 (ORM1, Orosomucoid 1), Genebank Accession Number: NM—000625 [NOS2A, Nitric oxide synthase 2A (inducible, hepatocytes)], Genebank Accession Number: NM—005515 (HLXB9, Homeo box HB9), Genebank Accession Number: BX537504 (C2, Complement component 2), Genebank Accession Number: BC028153 (LILRB3, Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3), Genebank Accession Number: AF130100 (SERPINC1, Serpin peptidase inhibitor, clade C (antithrombin), member 1), Genebank Accession Number: BC015110 (HHEX, Hematopoietically expressed homeobox), Genebank Accession Number: AB209179 [PLK1, Polo-like kinase 1 (Drosophila)], Genebank Accession Number: AY367065 [ASPM, Asp (abnormal spindle)-like, microcephaly associated (Drosophila)], Genebank Accession Number: NM—006101 (KNTC2, Kinetochore associated 2), Genebank Accession Number: NM—003550 [MAD1L1, MAD1 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—020242 (KNSL7, Kinesin family member 15), Genebank Accession Number: AB033068 [FZR1, Fizzy/cell division cycle 20 related 1 (Drosophila)], Genebank Accession Number: NM—018492 (PBK, PDZ binding kinase), Genebank Accession Number: NM—003318 (TTK, TTK protein kinase), Genebank Accession Number: BC011000 (CDCA5, Cell division cycle associated 5), Genebank Accession Number: NM—003981 (PRC1, Protein regulator of cytokinesis 1), Genebank Accession Number: NM—015097 (CLASP2, Cytoplasmic linker associated protein 2), Genebank Accession Number: U65410 [MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—016343 [CENPF, Centromere protein F, 350/400 ka (mitosin)], Genebank Accession Number: NM—005504 (BCAT1, Branched chain aminotransferase 1, cytosolic), Genebank Accession Number: NM—003718 [CDC2L5, Cell division cycle 2-like 5 (cholinesterase-related cell division controller)], Genebank Accession Number: NM—002768 (PCOLN3, Procollagen (type III) N-endopeptidase), Genebank Accession Number: BC004352 (KIF22, Kinesin family member 22), Genebank Accession Number: NM—005983 (SKP2, S-phase kinase-associated protein 2 (p45)], Genebank Accession Number: NM—004523 (KIF11, Kinesin family member 11), Genebank Accession Number: CR592757 [BRRN1, Barren homolog 1 (Drosophila)], Genebank Accession Number: NM—019063 (EML4, Echinoderm microtubule associated protein like 4), Genebank Accession Number: NM—006845 (KIF2C, Kinesin family member 2C), Genebank Accession Number: NM—004701 (CCNB2, Cyclin B2), Genebank Accession Number: NM—138555 (KIF23, Kinesin family member 23), Genebank Accession Number: NM—182687 (PKMYT1, Protein kinase, membrane associated tyrosine/threonine 1), Genebank Accession Number: NM—001790 (CDC25C, Cell division cycle 25C), Genebank Accession Number: BC032677 (UBE2C, Ubiquitin-conjugating enzyme E2C), Genebank Accession Number: NM—005886 (KATNB1, Katanin p80 (WD repeat containing) subunit B 1), Genebank Accession Number: BC073878 (KIFC1, Kinesin family member C1), Genebank Accession Number: AL833191 [SMC2L1, SMC2 structural maintenance of chromosomes 2-like 1 (yeast)], Genebank Accession Number: AF053305 [BUB1, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)], Genebank Accession Number: Z25431 (NEK1, NIMA (never in mitosis gene a)-related kinase 1), Genebank Accession Number: BC043371 [GFI1B, Growth factor independent 1B (potential regulator of CDKN1A, translocated in CML)], Genebank Accession Number: CR933728 (CDC2, Cell division cycle 2, G1 to S and G2 to M), Genebank Accession Number: NM—014750 [DLG7, Discs, large homolog 7 (Drosophila)], Genebank Accession Number: NM—018560 (WWOX, WW domain containing oxidoreductase), Genebank Accession Number: BG203702 (FHIT, Nasopharyngeal carcinoma, down-regulated 1), Genebank Accession Number: BC050384 [SUPT3H, Suppressor of Ty 3 homolog (S. cerevisiae)], Genebank Accession Number: NM—000189 (HK2, Hexokinase 2), Genebank Accession Number: CD049340 (AURKB, Aurora kinase B), Genebank Accession Number: AB011153 [PLCB1, Phospholipase C, beta 1 (phosphoinositide-specific)], Genebank Accession Number: AB209271 (ERF, Ets2 repressor factor), Genebank Accession Number: NM—014264 [PLK4, Polo-like kinase 4 (Drosophila)], Genebank Accession Number: NM—002417 (MKI67, Antigen identified by monoclonal antibody Ki-67), Genebank Accession Number: XM—496557 (RGP1, Plasminogen-like B2), Genebank Accession Number: NM—170715 (RASSF1, Ras association (RalGDS/AF-6) domain family 1), Genebank Accession Number: NM—004526 [MCM2, MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)], Genebank Accession Number: BC033086 [TCF19, Transcription factor 19 (SC1)], Genebank Accession Number: NM—001007071 (RPS6KB2, Ribosomal protein S6 kinase, 70 kDa, polypeptide 2), Genebank Accession Number: NM—016272 (TOB2, Transducer of ERBB2, 2), Genebank Accession Number: AK126688 (CDYL2, Chromodomain protein, Y-like 2), Genebank Accession Number: NM—001759 (CCND2, Cyclin D2), Genebank Accession Number: NM—002388 [MCM3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)], Genebank Accession Number: NM—182776 [MCM7, MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)], Genebank Accession Number: NM—012145 [DTYMK, Deoxythymidylate kinase (thymidylate kinase)], Genebank Accession Number: NM—014751 (MTSS1, Metastasis suppressor 1), Genebank Accession Number: NM—024342 (GRLF1, Glucocorticoid receptor DNA binding factor 1), Genebank Accession Number: AK025627 (CABLES1, Cdk5 and Abl enzyme substrate 1), Genebank Accession Number: NM—005915 [MCM6, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae)], Genebank Accession Number: AF196185 [PARD3, Par-3 partitioning defective 3 homolog (C. elegans)], Genebank Accession Number: NM—019001 (XRN1, 5′-3′ exoribonuclease 1), Genebank Accession Number: NM—006739 [MCM5, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)], Genebank Accession Number: NM—012218 (ILF3, Interleukin enhancer binding factor 3, 90 kDa), Genebank Accession Number: AB053172 (CDT1, DNA replication factor), Genebank Accession Number: AK074112 (CCNL2, Cyclin L2), Genebank Accession Number: NM—001005414 (ZWINT, ZW10 interactor antisense), Genebank Accession Number: BC033391 (PP1665, Glycerophosphodiester phosphodiesterase domain containing 5), Genebank Accession Number: NM—000672 [ADH6, Alcohol dehydrogenase 6 (class V)], Genebank Accession Number: NM—004563 [PCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial)], Genebank Accession Number: XM—113962 (KIAA0650, Structural maintenance of chromosomes flexible hinge domain containing 1), Genebank Accession Number: NM—001500 (GMDS, GDP-mannose 4,6-dehydratase), Genebank Accession Number: NM—002541 [OGDH, Oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)], Genebank Accession Number: NM—000298 (PKLR, Pyruvate kinase, liver and RBC), Genebank Accession Number: NM—005276 [GPD1, Glycerol-3-phosphate dehydrogenase 1 (soluble)], Genebank Accession Number: AB209127 (PFKL, Phosphofructokinase, liver), Genebank Accession Number: M15943 (ADH4, Alcohol dehydrogenase 4 (class II), pi polypeptide), Genebank Accession Number: NM—000671 (ADH5, Alcohol dehydrogenase 5 (class III), chi polypeptide), Genebank Accession Number: BC026320 (OGDHL, Oxoglutarate dehydrogenase-like), Genebank Accession Number: NM—009590 [AOC2, Amine oxidase, copper containing 2 (retina-specific)], Genebank Accession Number: BC015797 (SLC25A10, Solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10), Genebank Accession Number: NM—003748 (ALDH4A1, Aldehyde dehydrogenase 4 family, member A1), Genebank Accession Number: NM—002108 (HAL, Histidine ammonia-lyase), Genebank Accession Number: NM—003486 (SLC7A5, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 5), Genebank Accession Number: NM—003045 (SLC7A1, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 1), Genebank Accession Number: NM—000277 (PAH, Phenylalanine hydroxylase), Genebank Accession Number: NM—012331 (MSRA, Methionine sulfoxide reductase A), Genebank Accession Number: BC008366 [DDC, Dopa decarboxylase (aromatic L-amino acid decarboxylase)], Genebank Accession Number: NM—006567 [FARS2, Phenylalanine-tRNA synthetase 2 (mitochondrial)], Genebank Accession Number: AK055862 (FLJ31300, Urocanase domain containing 1), Genebank Accession Number: NM—001875 (CPS1, Carbamoyl-phosphate synthetase 1, mitochondrial), Genebank Accession Number: AK122685 (GLUD1, Glutamate dehydrogenase 1), Genebank Accession Number: NM—003046 (SLC7A2, Solute carrier family 7 (cationic amino acid transporter, y+ system), member 2), Genebank Accession Number: AK093306 (PHGDH, Phosphoglycerate dehydrogenase), Genebank Accession Number: NM—080820 (HARS2, Histidyl-tRNA synthetase 2), Genebank Accession Number: NM—006907 (PYCR1, Pyrroline-5-carboxylate reductase 1), Genebank Accession Number: NM—020117 (LARS, Leucyl-tRNA synthetase), Genebank Accession Number: BC051726 [GLUL, Glutamate-ammonia ligase (glutamine synthetase)], Genebank Accession Number: BC012616 (BHMT, Betaine-homocysteine methyltransferase), Genebank Accession Number: AK055053 [SHMT2, Serine hydroxymethyltransferase 2 (mitochondrial)], Genebank Accession Number: NM—007101 (SARDH, Sarcosine dehydrogenase), Genebank Accession Number: AL833251 (LOC283970, Hypothetical protein LOC283970), Genebank Accession Number: AK027126 (ASS, Argininosuccinate synthetase), Genebank Accession Number: L00972 (CBS, Cystathionine-beta-synthase), Genebank Accession Number: NM—206965 (FTCD, Formiminotransferase cyclodeaminase), Genebank Accession Number: NM—000429 (MAT1A, Methionine adenosyltransferase I, alpha), Genebank Accession Number: NM—001801 (CDO1, Cysteine dioxygenase, type I), Genebank Accession Number: NM—006843 (SDS, Serine dehydratase), Genebank Accession Number: NM—000170 [GLDC, Glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavage system protein P)], Genebank Accession Number: BX102654 (HIST1H4B, Histone 1, H4b), Genebank Accession Number: DQ097177 (UREB1, HECT, UBA and WWE domain containing 1), Genebank Accession Number: NM—022743 (SMYD3, SET and MYND domain containing 3), Genebank Accession Number: NM—001809 (CENPA, Centromere protein A, 17 kDa), Genebank Accession Number: AB028450 (NCOR1, Nuclear receptor co-repressor 1), Genebank Accession Number: NM—002967 (SAFB, Scaffold attachment factor B), Genebank Accession Number: NM—003074 (SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1), Genebank Accession Number: NM—015409 (EP400, E1A binding protein p400), Genebank Accession Number: BC044659 (SMARCA3, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3), Genebank Accession Number: AB209433 (BAT8, Euchromatic histone-lysine N-methyltransferase 2), Genebank Accession Number: XM—370756 (KIAA1305, KIAA1305), Genebank Accession Number: NM—020201 (NT5M, 5′,3′-nucleotidase, mitochondrial), Genebank Accession Number: NM—001025248 (DUT, DUTP pyrophosphatase), Genebank Accession Number: AK123010 (RRM2, Ribonucleotide reductase M2 polypeptide), Genebank Accession Number: BX537961 (DNMT2, DNA (cytosine-5-)-methyltransferase 2), Genebank Accession Number: BX648935 (TBL1XR1, Transducin (beta)-like 1X-linked receptor 1), Genebank Accession Number: NM—001001888 (VCX-C, Variably charged X-C), Genebank Accession Number: NM—002501 [NFIX, Nuclear factor I/X (CCAAT-binding transcription factor)], Genebank Accession Number: NM—003483 (HMGA2, High mobility group AT-hook 2), Genebank Accession Number: U80628 (TK2, Thymidine kinase 2, mitochondrial), Genebank Accession Number: NM—006807 [CBX1, Chromobox homolog 1 (HP1 beta homolog Drosophila)], Genebank Accession Number: AY024361 (MLL3, Myeloid/lymphoid or mixed-lineage leukemia 3), Genebank Accession Number: NM—017519 [ARID1B, AT rich interactive domain 1B (SWI1-like)], Genebank Accession Number: NM—003077 (SMARCD2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2), Genebank Accession Number: NM—016937 (POLA, Polymerase (DNA directed), alpha), Genebank Accession Number: AJ007583 (LARGE, Like-glycosyltransferase), Genebank Accession Number: AK124635 (PCSK9, Proprotein convertase subtilisin/kexin type 9), Genebank Accession Number: NM—030758 (OSBP2, Oxysterol binding protein 2), Genebank Accession Number: NM—005063 [SCD, Stearoyl-CoA desaturase (delta-9-desaturase)], Genebank Accession Number: NM—000236 (LIPC, Lipase, hepatic), Genebank Accession Number: AF288389 (GLT25D2, Glycosyltransferase 25 domain containing 2), Genebank Accession Number: AF202889 (APOA5, Apolipoprotein A-V), Genebank Accession Number: BX640945 (FADS2, Fatty acid desaturase 2), Genebank Accession Number: BI521580 (APOC3, Apolipoprotein C-III), Genebank Accession Number: NM—001277 (CHKA, Choline kinase alpha), Genebank Accession Number: Z28339 [AKR1D1, Aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)], Genebank Accession Number: BC035654 (NR1H4, Nuclear receptor subfamily 1, group H, member 4), Genebank Accession Number: AB209229 (MVD, Mevalonate (diphospho) decarboxylase), Genebank Accession Number: BC026264 (UGT2B4, UDP glucuronosyltransferase 2 family, polypeptide B4), Genebank Accession Number: NM—004104 (FASN, Fatty acid synthase), Genebank Accession Number: NM—147161 (THEA, Acyl-CoA thioesterase 11), Genebank Accession Number: AF059203 (SOAT2, Sterol O-acyltransferase 2), Genebank Accession Number: NM—020918 (GPAM, Glycerol-3-phosphate acyltransferase, mitochondrial), Genebank Accession Number: NM—001443 (FABP1, Fatty acid binding protein 1, liver), Genebank Accession Number: NM—014762 (DHCR24, 24-dehydrocholesterol reductase), Genebank Accession Number: AK093328 (PCYT2, Phosphate cytidylyltransferase 2, ethanolamine), Genebank Accession Number: BX538214 (C6orf167, Chromosome 6open reading frame 167), Genebank Accession Number: X75311 [MVK, Mevalonate kinase (mevalonic aciduria)], Genebank Accession Number: NM—000497 (CYP11B1, Cytochrome P450, family 11, subfamily B, polypeptide 1), Genebank Accession Number: BC036102 (SIAT7F, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6), Genebank Accession Number: BC012172 (ACAS2, Acyl-CoA synthetase short-chain family member 2), Genebank Accession Number: NM—007169 (PEMT, Phosphatidylethanolamine N-methyltransferase), Genebank Accession Number: X91148 (MTP, Microsomal triglyceride transfer protein), Genebank Accession Number: NM—198839 (ACACA, Acetyl-Coenzyme A carboxylase alpha), Genebank Accession Number: NM—004687 (MTMR4, Myotubularin related protein 4), Genebank Accession Number: NM—000384 [APOB, Apolipoprotein B (including Ag (x) antigen)], Genebank Accession Number: AK127051 [ACAA1, Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-oxoacyl-Coenzyme A thiolase)], Genebank Accession Number: BC035638 [LSS, Lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)], Genebank Accession Number: NM—004204 (PIGQ, Phosphatidylinositol glycan, class Q), Genebank Accession Number: NM—004457 (ACSL3, Acyl-CoA synthetase long-chain family member 3), Genebank Accession Number: AL138578 (ADPN, Adiponutrin), Genebank Accession Number: NM—002332 [LRP1, Low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)], Genebank Accession Number: NM—016372 (GPR175, G protein-coupled receptor 175), Genebank Accession Number: NM—080476 [CDC91L1, CDC91 cell division cycle 91-like 1 (S. cerevisiae)], Genebank Accession Number: AF303134 (ALDH8A1, Aldehyde dehydrogenase 8 family, member A1), Genebank Accession Number: NM—002130 [HMGCS1, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)], Genebank Accession Number: AK055414 [LASS4, LAG1 longevity assurance homolog 4 (S. cerevisiae)], Genebank Accession Number: NM—022977 (ACSL4, Acyl-CoA synthetase long-chain family member 4), Genebank Accession Number: NM—004631 (LRP8, Low density lipoprotein receptor-related protein 8, apolipoprotein e receptor), Genebank Accession Number: NM—002573 (PAFAH1B3, Platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29 kDa), Genebank Accession Number: NM—021005 (NR2F2, Nuclear receptor subfamily 2, group F, member 2), Genebank Accession Number: CR620135 (DPEP1, Dipeptidase 1 (renal)], Genebank Accession Number: NM—002317 (LOX, Lysyl oxidase), Genebank Accession Number: NM—005949 [MT1F, Metallothionein 1F (functional)], Genebank Accession Number: AF279145 (ANTXR1, Anthrax toxin receptor 1), Genebank Accession Number: NM—005588 [MEP1A, Meprin A, alpha (PABA peptide hydrolase)], Genebank Accession Number: NM—014214 (IMPA2, Inositol (myo)-1 (or 4)-monophosphatase 2), Genebank Accession Number: NM—005940 [MMP11, Matrix metallopeptidase 11 (stromelysin 3)], Genebank Accession Number: AB193259 (ACE2, Angiotensin I converting enzyme (peptidyl-dipeptidase A) 2), Genebank Accession Number: AF018081 (COL18A1, Collagen, type XVIII, alpha 1), Genebank Accession Number: M28016 (cytochrome b, Human mitochondrial cytochrome b gene, partial cds.), Genebank Accession Number: NM—002428 [MMP15, Matrix metallopeptidase 15 (membrane-inserted)], Genebank Accession Number: NM—025074 (FRAS1, Fraser syndrome 1), Genebank Accession Number: M75106 [CPB2, Carboxypeptidase B2 (plasma, carboxypeptidase U)], Genebank Accession Number: NM—000937 (POLR2A, Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa), Genebank Accession Number: NM—005060 (RORC, RAR-related orphan receptor C), Genebank Accession Number: BC092512 (MYRIP, Myosin VIIA and Rab interacting protein), Genebank Accession Number: NM—000901 (NR3C2, Nuclear receptor subfamily 3, group C, member 2), Genebank Accession Number: NM—005392 (PHF2, PHD finger protein 2), Genebank Accession Number: AK091289 (ZNF367, Zinc finger protein 367), Genebank Accession Number: NM—014788 (TRIM14, Tripartite motif-containing 14), Genebank Accession Number: NM—018660 (ZNF395, Zinc finger protein 395), Genebank Accession Number: AB209755 (MLLT10, Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10), Genebank Accession Number: AK097472 [MAZ, MYC-associated zinc finger protein (purine-binding transcription factor)], Genebank Accession Number: NM—020062 (SLC2A4RG, SLC2A4 regulator), Genebank Accession Number: BX571750 (HNF4G, Hepatocyte nuclear factor 4, gamma), Genebank Accession Number: NM—021807 (SEC8L1, Exocyst complex component 4), Genebank Accession Number: NM—006651 (CPLX1, Complexin 1), Genebank Accession Number: NM—005845 (ABCC4, ATP-binding cassette, sub-family C (CFTR/MRP), member 4), Genebank Accession Number: NM—006650 (CPLX2, Complexin 2), Genebank Accession Number: NM—004164 (RBP2, Retinol binding protein 2, cellular), Genebank Accession Number: NM—021069 (ARGBP2, Sorbin and SH3 domain containing 2), Genebank Accession Number: NM—201649 (SLC6A9, Solute carrier family 6 (neurotransmitter transporter, glycine), member 9), Genebank Accession Number: NM—005835 (SLC17A2, Solute carrier family 17 (sodium phosphate), member 2), Genebank Accession Number: AJ315644 (SLC2A13, Solute carrier family 2 (facilitated glucose transporter), member 13), Genebank Accession Number: NM—000340 (SLC2A2, Solute carrier family 2 (facilitated glucose transporter), member 2), Genebank Accession Number: NM—003685 [KHSRP, KH-type splicing regulatory protein (FUSE binding protein 2)], Genebank Accession Number: BX537382 (SLC38A3, Solute carrier family 38, member 3), Genebank Accession Number: NM—182964 (NAV2, Neuron navigator 2), Genebank Accession Number: BX640965 (SLC22A3, Solute carrier family 22 (extraneuronal monoamine transporter), member 3), Genebank Accession Number: AK127255 (SNX26, Sorting nexin 26), Genebank Accession Number: AY043484 (SCN1A, Sodium channel, voltage-gated, type I, alpha), Genebank Accession Number: AK074107 (C20orf59, Chromosome 20 open reading frame 59), Genebank Accession Number: NM—003056 (SLC19A1, Solute carrier family 19 (folate transporter), member 1), Genebank Accession Number: NM—020897 (HCN3, Hyperpolarization activated cyclic nucleotide-gated potassium channel 3), Genebank Accession Number: AY124771 (SLC26A1, Solute carrier family 26 (sulfate transporter), member 1), Genebank Accession Number: NM—001011554 (SLC13A3, Solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3), Genebank Accession Number: AK027855 (SLC5A6, Solute carrier family 5 (sodium-dependent vitamin transporter), member 6), Genebank Accession Number: NM—001005747 (CACNB4, Calcium channel, voltage-dependent, beta 4 subunit), Genebank Accession Number: NM—013277 (RACGAP1, Rac GTPase activating protein 1), Genebank Accession Number: NM—012340 (NFATC2, Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), Genebank Accession Number: NM—018291 (FLJ10986, Hypothetical protein FLJ10986), Genebank Accession Number: BC052951 (LMNB1, Lamin B1), Genebank Accession Number: AK075271 [SPTLC2L, Serine palmitoyltransferase, long chain base subunit 2-like (aminotransferase 2)], Genebank Accession Number: BI762502 (GSTA2, Glutathione S-transferase A2), Genebank Accession Number: AK055221 (FBXO5, F-box protein 5), Genebank Accession Number: XM—372695 (LOC390859, PREDICTED: Homo sapiens similar to Chain A, Crystal Structure Of The R463a Mutant Of Human Glutamate Dehydrogenase (LOC390859), mRNA), Genebank Accession Number: AB037805 [KLHL14, Kelch-like 14 (Drosophila)], Genebank Accession Number: NM—145061 (C13orf3, Chromosome 13 open reading frame 3), Genebank Accession Number: AK000643 (ASB9, Ankyrin repeat and SOCS box-containing 9), Genebank Accession Number: AL133031 (MLR1, Transcription factor MLR1), Genebank Accession Number: AB091343 (C10orf3, Centrosomal protein 55 kDa), Genebank Accession Number: AY007723 (MAL2, Mal, T-cell differentiation protein 2), Genebank Accession Number: BC098313 (FLJ20641, Hypothetical protein FLJ20641), Genebank Accession Number: BC041882 (ATF7IP2, Activating transcription factor 7 interacting protein 2), Genebank Accession Number: AK127098 (FLJ10706, Chromosome 1 open reading frame 112), Genebank Accession Number: BC022255 [Spc25, Spindle pole body component 25 homolog (S. cerevisiae)], and Genebank Accession Number: BC026283 [APOH, Apolipoprotein H (beta-2-glycoprotein I)].
To find out the gene whose expression is changed by chrysene exposure, the human hepatoma cell line HepG2 was treated with chrysene and cytotoxicity was confirmed. As a result, chrysene demonstrated cytotoxicity in the human hepatoma cell line (see FIG. 1). Based on the experiment, chrysene concentration exhibiting 80% cell survival rate (IC20) was determined.
The human Hepatoma cell line was treated with chrysene by the determined concentration and mRNA was extracted therefrom. During cDNA synthesis, it was labeled with Cy5. The control group not treated with the chrysene was labeled with Cy3. The fluorescence-labeled cDNA was hybridized with the 44 k human whole genome oligo-microarray chip (Agilent, USA), followed by scanning of fluorescent images to analyze the gene expression patterns (see FIG. 2). When the margin of the ratio of Cy5 to Cy3 was more than 2.0 fold, the gene expression was considered to be increased so that the gene was classified into the up-regulated gene group. When the margin of the ratio of Cy5 to Cy3 was less than 0.5 fold, the gene expression was considered to be decreased so that the gene was classified into the down-regulated gene group. As a result, the gene identified to be up-regulated was 0.73% (323 genes out of 44,000 genes) and the gene identified to be down-regulated was 0.82% (361 genes out of 44,000 genes). At this time, the genes exhibiting 2.0 fold higher or less expression by chrysene exposure were classified according to the functions. The genes are composed of those genes involved in MAPKinase Signaling Pathway, MAPKKK cascade, p53 Signaling Pathway, apoptosis, response to DNA damage stimulus, Response to stress, immune response, cell cycle, Mitotic cell cycle, Cell differentiation, Cell growth, cell proliferation, Alcohol metabolism, DNA metabolism, lipid metabolism, Amino acid metabolism, metal binding, and Transport (see Table 2 and Table 3). There have been no reports that those selected genes are involved in cytotoxicity in human Hepatoma cells according to the chrysene treatment.
The present inventors separated 25 over-expressed genes and 10 under-expressed genes. Then, primers were designed to amplify those genes, followed by real time RT-PCR (real-time reverse transcript polymerase chain reaction) to examine their expression patterns.
As a result, 25 up-regulated genes and 10 down-regulated genes were detected, consistent with the result of the experiment using the oligo-microarray chip (see Table 5). The genes are Genebank Accession Number: NM—000499 (Cytochrome P450, family 1, subfamily A, polypeptide 1), Genebank Accession Number: BX649164 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1), Genebank Accession Number: NM—004881 (Tumor protein p53 inducible protein 3), Genebank Accession Number: BM913048 (TIMP metallopeptidase inhibitor 1), Genebank Accession Number: NM—006426 (Dihydropyrimidinase-like 4), Genebank Accession Number: NM—133493 [CD109 antigen (Gov platelet alloantigens)], Genebank Accession Number: AB033073 (Sulfatase 2), Genebank Accession Number: NM—021603 (FXYD domain containing ion transport regulator 2), Genebank Accession Number: BC035124 (MSFL2541), Genebank Accession Number: Y11307 (Cysteine-rich, angiogenic inducer, 61), Genebank Accession Number: AK095363 (Aquaporin 3), Genebank Accession Number: AK097009 (phospholipid transfer protein), Genebank Accession Number: NM—004864 (Growth differentiation factor 15), Genebank Accession Number: BU587578 (Regulator of G-protein signaling 10), Genebank Accession Number: BQ683841 [S100 calcium binding protein A11 (calgizzarin)], Genebank Accession Number: NM—001013632 [polo-like kinase 3 (Drosophila)], Genebank Accession Number: NM—032872 (Synaptotagmin-like 1), Genebank Accession Number: BC042390 [Vesicle transport through interaction with t-SNAREs homolog 1B (yeast)], Genebank Accession Number: NM—000596 (Insulin-like growth factor binding protein 1), Genebank Accession Number: NM—005319 (Histone 1, H1c), Genebank Accession Number: AK094474 (Ras homolog gene family, member C), Genebank Accession Number: NM—001769 [CD9 antigen (p24)], Genebank Accession Number: NM—001553 (Insulin-like growth factor binding protein 7), Genebank Accession Number: NM—003897 (Immediate early response 3), Genebank Accession Number: NM—003516 (Histone 2, H2aa), Genebank Accession Number: AK074377 (Chromosome 19 open reading frame 31), Genebank Accession Number: BX640965 (Solute carrier family 22 (extraneuronal monoamine transporter), member 3), Genebank Accession Number: NM—018492 (PDZ binding kinase), Genebank Accession Number: CR600021 (High-mobility group box 2), Genebank Accession Number: NM—006101 (Kinetochore associated 2), Genebank Accession Number: NM—003318 (TTK protein kinase), Genebank Accession Number: U65410 [MAD2 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—001067 (Topoisomerase (DNA) II alpha 170 kDa), Genebank Accession Number: AB091343 (Centrosomal protein 55 kDa), and Genebank Accession Number: BC022255 [Spindle pole body component 25 homolog (S. cerevisiae)].
MODE FOR INVENTION
Practical and presently preferred embodiments of the present invention are illustrative as shown in the following Examples.
However, it will be appreciated that those skilled in the art, on consideration of this disclosure, may make modifications and improvements within the spirit and scope of the present invention.
Example 1
Cell Culture and Chemical Treatment
<1-1> Cell Culture
The human hepatoma cell line HepG2 (Korean Cell Line Bank) was cultured in DMEM (Gibco-BRL, USA) supplemented with 10% FBS in 100 mm dish until it grew 80%. The present inventors selected chrysene (Sigma-Aldrich, USA) among polycyclic aromatic hydrocarbons known to have carcinogenicity, based on the previous studies and reports. Chrysene was dissolved in DMSO. The concentration of vehicle was up to 0.1% in every experiment.
<1-2> Cytotoxicity Assay (MTT Assay) and Chemical Treatment
MTT assay with the HepG2 cell line was performed according to the method of Mossman et al. (J. Immunol. Methods, 65: 55-63, 1983). The cells were inoculated in a 24-well plate containing DMEM at the concentration of 4×105 cells/well, to which chrysene dissolved in DMSO was treated. 48 hours later, MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetra zolium bromide) was added at the concentration of 5 mg/ml, followed by culture at 37° C. for 3 hours. Then, medium was eliminated and generated formazan crystal was dissolved in 500 μl of DMSO. The solution was aliquoted in a 96 well-plate and OD540 was measured. Cytotoxicity in the HepG2 cell line caused by chrysene was investigated. As a result, IC20 was 34.97□M (see FIG. 1) and thus the concentration of chrysene for the further experiment was determined as the above.
Example 2
Microarray Experiment
<2-1> Separation of a Target RNA and Labeling with a Fluorescent Material
HepG2 cells were distributed on a 100 mm dish at the concentration of 6×106 cell/ml, to which chrysene was treated for 48 hours at the concentration that was determined in Example <1-2>. Total RNA was extracted from the cells using trizol reagent (Invitrogen life technologies, USA) according to the manufacturer's instruction and the extracted RNA was purified by using RNeasy mini kit (Qiagen, USA). The genomic DNA was eliminated during the purification of RNA using RNase-free DNase set (Qiagen, USA). The total RNA was quantified by spectrophotometer and purity was measured by Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and agarose gel-electrophoration.
<2-2> Preparation of Labeled cDNA
For oligo-microarray analysis, cDNA was synthesized using the total RNA extracted from the experimental group treated with chrysene obtained in Example <2-1>. 30 μg of the total RNA and 2 μg (1 μg/μl) of oligo (dT) primer were mixed, followed by reaction for 10 minutes at 65° C. Then, the reaction mixture was put in ice, followed by annealing. Reagents were mixed as shown in Table 1 for reverse transcription of the RNA.










TABLE 1







Composition
volume (μl)



















5X first strand buffer
6



dNTPs
0.6



0.1 M DDT
3



SuperScript II enzyme
3



Cy-3 or Cy-5 dUTP
2










The total RNA extracted from the control HepG2 cell line was labeled with Cy3-dUTP (green), while the RNA extracted from the experimental HepG2 cell line treated with chrysene was labeled with Cy5-dUTP (red). At this time, the two samples were mixed and purified by Microcon YM-30 column (Millipore, USA).
<2-3> Hybridization
Hybridization and washing were performed according to the instruction of GeneoCheck Co., Ltd. Hybridization was performed in a 62° C. oven for 12 hours. At this time, 44 k whole human genome oligo microarray (Agilent, USA) was used as a DNA microarray chip. After washing (with 2×SSC/0.1% SDS for 2 minutes, with 1×SSC for 3 minutes, with 0.2×SSC for 2 minutes), the slide was centrifuged at 800 rpm for 3 minutes and dried.
<2-4> Fluorescence Image Obtaining
Scanning of the hybridized images on the slide was performed by using Genepix 4000B (Axon Instruments, USA). Fluorescence images of the chip washed to eliminate non-binding genes were obtained by laser fluorescence scanner. At this time, green fluorescent images indicated the activity of the gene expressed in the control, whereas red fluorescent images indicated the activity of the gene expressed specifically in the experimental group. In the meantime, yellow fluorescent images (complementary color of red and green) indicated that there was not much difference in the expression between the two groups. The scanned images were analyzed by GenePix 4.1 software (Axon Instruments, USA) to calculate the gene expression rate. Based on the obtained data, marker genes for chrysene were selected (see FIG. 2).
As a result, the gene identified to be 2.0 fold higher up-regulated was 0.73% (323 genes out of 44,000 genes) and the gene identified to be 2.0 fold less down-regulated was 0.82% (361 genes out of 44,000 genes) among approximately 44,000 genes loaded on the oligo chip.
At this time, genes exhibiting 2.0 fold higher or less expressions by exposure to chrysene were classified according to the functions. The genes are composed of those genes involved in MAPKinase Signaling Pathway, MAPKKK cascade, p53 Signaling Pathway, apoptosis, response to DNA damage stimulus, Response to stress, immune response, cell cycle, Mitotic cell cycle, Cell differentiation, Cell growth, cell proliferation, Alcohol metabolism, DNA metabolism, lipid metabolism, Amino acid metabolism, metal binding, and Transport (see Table 2 and Table 3). There have been no reports so far that those selected genes are involved in cytotoxicity in human Hepatoma cells according to the chrysene treatment.







TABLE 2







Genes up-regulated by chrysene













Ratio of


Accession
Gene

intermediate


number
abbreviation
Gene name
value










(a) MAPKinase Signaling Pathway










NM_003954
MAP3K14
Mitogen-activated protein kinase kinase
2.642090915




kinase 14


AB209586
MAPK13
Mitogen-activated protein kinase 13
2.32604791


NM_005343
HRAS
V-Ha-ras Harvey rat sarcoma viral oncogene
2.300183763




homolog


BX647104
FOS
V-fos FBJ murine osteosarcoma viral
2.286644731




oncogene homolog


NM_007315
STAT1
Signal transducer and activator of
2.268249473




transcription 1, 91 kDa


BC027933
MAPK11
Mitogen-activated protein kinase 11
2.256117014


NM_020529
NFKBIA
Nuclear factor of kappa light polypeptide
2.242341043




gene enhancer in B-cells inhibitor, alpha


BC014966
RPS6KA1
Ribosomal protein S6 kinase, 90 kDa,
2.109933874




polypeptide 1







(b) p53 Signaling Pathway










NM_181861
APAF1
Apoptotic peptidase activating factor
2.323610287


L41870
RB1
Retinoblastoma 1 (including osteosarcoma)
2.431277919


CR612719
GADD45A
Growth arrest and DNA-damage-inducible,
2.785095956




alpha


BM462208
PCNA
Proliferating cell nuclear antigen
5.94704061


M92424
MDM2
Mdm2, transformed 3T3 cell double minute
4.056640879




2, p53 binding protein(mouse)


NM_078467
CDKN1A
Cyclin-dependent kinase inhibitor1A(p21,
21.05152341




Cip1)







(c) Apoptosis










BC089389
IHPK3
Inositol hexaphosphate kinase 3
17.86623375


NM_001024807
APLP1
Amyloid beta(A4) precursor-like protein 1
14.60038469


NM_001013398
IGFBP3
Insulin-like growth factor binding
11.51469533




protein 3


AB209361
FAS
Fas(TNF receptor superfamily, member 6)
9.282797498


NM_147780
CTSB
Cathepsin B
6.625352723


NM_001165
BIRC3
Baculoviral IAP repeat-containing 3
6.105445052


BM557864
HSPB1
Heat shock 27 kDa protein 1
5.988972006


AB033060
PDCD6
Aryl-hydrocarbon receptor repressor
5.709323333


AK125880
TP53INP1
Tumor protein p53 inducible nuclear
4.730741529




protein 1


NM_004574
04-Sep
Septin 4
4.420718644


NM_003820
TNFRSF14
Tumor necrosis factor receptor
4.241834154




superfamily, member 14(herpesvirus entry




mediator)


CN478604
LGALS7
Lectin, galactoside-binding, soluble,
4.215529017




7(galectin 7)


NM_004879
EI24
Etoposide induced 2.4 mRNA
3.840012717


NM_003311
PHLDA2
Pleckstrin homology-like domain, family
3.66045378




A, member 2


NM_003897
IER3
Immediate early response 3
3.323689948


AK097284
TNFAIP8
Tumor necrosis factor, alpha-induced
3.301416171




protein 8


NM_000582
SPP1
Secreted phosphoprotein 1(osteopontin,
3.007301243




bone sialoprotein I, early T-lymphocyte




activation 1)


NM_013258
PYCARD
PYD and CARD domain containing
2.704730239


NM_002305
LGALS1
Lectin, galactoside-binding, soluble,
2.685571797




1(galectin 1)


AF055634
UNC5C
Unc-5 homolog C(C. elegans)
2.64453651


CR614015
CD14
CD14 antigen
2.620138457


AK129595
GADD45B
Growth arrest and DNA-damage-inducible,
2.431892144




beta


AF356193
CARD6
Caspase recruitment domain family, member 6
2.315498432


BC063043
PRF1
Perforin 1(pore forming protein)
2.300936607


BX537586
STK17A
Serine/threonine kinase
2.173582084




17a(apoptosis-inducing)


NM_003842
TNFRSF10B
Tumor necrosis factor receptor
2.164540104




superfamily, member 10b


AF208043
IFI16
Interferon, gamma-inducible protein 16
2.147368247


BX641114
ANXA4
Annexin A4
2.143018533


NM_001009552
PPP2CB
Protein phosphatase 2 (formerly 2A),
2.132316431




catalytic subunit, beta isoform


BX386171
CGB5
Chorionic gonadotropin, beta polypeptide 8
2.11678504


AY358103
HIP1
Huntingtin interacting protein 1
2.101135287


BC027949
P2RX1
Purinergic receptor P2X, ligand-gated ion
2.093924251




channel, 1


BC042844
CASP10
Caspase 10, apoptosis-related cysteine
2.071460864




peptidase


NM_000733
CD3E
CD3E antigen, epsilon polypeptide(TiT3
2.069295152




complex)


NM_033245
PML
Promyelocytic leukemia
2.031289487


BC047362
PHLDA1
Pleckstrin homology-like domain, family
2.030766246




A, member 1







(d) Response to DNA damage stimulus










NM_006763
BTG2
BTG family, member 2
8.033431013


BC050455
DDB2
LIM homeobox 3
5.130890941


BC068535
TP53AP1
TP53 activated protein 1
3.847202974


AF033122
SESN1
Sestrin 1
3.728462177


AB024313
POLH
Polymerase(DNA directed), eta
3.515123881


NM_001012716
TYMS
Thymidylate synthetase
3.123891212


BC095402
ATXN3
Ataxin 3
2.201860506


BU675073
PTTG1
Pituitary tumor-transforming 1
2.178628404


BC073161
RAD51C
RAD51 homolog C(S. cerevisiae)
2.092699518


NM_001983
ERCC1
Excision repair cross-complementing
1.811298942




rodent repair deficiency, complementation




group 1 (includes overlapping antisense




sequence)







(e) Response to stress










NM_018965
TREM2
Triggering receptor expressed on myeloid
43.66510076




cells 2


BX649164
PAI; PAI1;
serpin peptidase inhibitor, clade
29.04597327



PAI-1;
E(nexin, plasminogen activator inhibitor



PLANH1
type 1), member 1


NM_006404
PROCR
Protein C receptor, endothelial(EPCR)
24.52788989


BC035582
TRIM22
Tripartite motif-containing 22
13.78919459


NM_006691
XLKD1
Extracellular link domain containing 1
10.05591814


NM_015831
ACHE
Acetylcholinesterase (YT blood group)
6.223349052


BC045666
TP53I11
Tumor protein p53 inducible protein 11
6.007745545


NM_005438
FOSL1
FOS-like antigen 1
5.642502876


NM_005755
EBI3
Epstein-Barr virus induced gene 3
5.606891978


BM918324
LY96
Lymphocyte antigen 96
5.438550593


NM_000022
ADA
Adenosine deaminase
5.212717207


NM_004167
CCL14
Chemokine(C-C motif) ligand 15
5.102559642


NM_014278
HSPA4L
Heat shock 70 kDa protein 4-like
4.710398517


BC006523
SGK2
Serum/glucocorticoid regulated kinase 2
4.64953324


NM_000641
IL11
Interleukin 11
4.520629217


H18681
C20orf139
Sulfiredoxin 1 homolog(S. cerevisiae)
4.383369833


NM_001769
CD9
CD9 antigen(p24)
4.153073307


BG564326
SAA4
Serum amyloid A4, constitutive
4.075835655


NM_016816
OAS1
2′,5′-oligoadenylate synthetase 1,
3.710610826




40/46 kDa


CB055213
ICOSLG
Inducible T-cell co-stimulator ligand
3.643779829


CR591007
CCL3
Chemokine(C-C motif) ligand 3
3.346884519


NM_145641
APOL3
Apolipoprotein L, 3
3.195513004


AK127663
PTGES
Prostaglandin E synthase
3.183821649


NM_001734
C1S
Complement component 1, s subcomponent
3.018342133


NM_002260
KLRC3
Killer cell lectin-like receptor
2.941895363




subfamily C, member 2


NM_001733
C1R
Complement component 1, r subcomponent
2.893399755


AB209922
DNAJB4
DnaJ(Hsp40) homolog, subfamily B, member 4
2.872000914


NM_201397
GPX1
Glutathione peroxidase 1
2.850015447


AK090482
CXCL12
Chemokine(C-X-C motif) ligand 12(stromal
2.842225911




cell-derived factor 1)


BF662985
CCL3L1
Chemokine(C-C motif) ligand 3-like 1
2.838582233


NM_001779
CD58
CD58 antigen, (lymphocyte
2.794341637




function-associated antigen 3)


NM_005064
CCL23
Chemokine(C-C motif) ligand 23
2.761400386


NM_000242
MBL2
Mannose-binding lectin(protein C) 2,
2.755428358




soluble(opsonic defect)


NM_032036
FAM14A
Family with sequence similarity 14, member A
2.706203775


AJ890082
HSPCA
Heat shock 90 kDa protein 1, alpha
2.54395008


BC068487
TLR3
Toll-like receptor 3
2.540522037


NM_000552
VWF
Von Willebrand factor
2.476094941


NM_000132
F8
Coagulation factor VIII, procoagulant
2.414521007




component(hemophilia A)


AY154461
NALP6
NACHT, leucine rich repeat and PYD
2.392028958




containing 6


AK127679
DUSP1
Dual specificity phosphatase 1
2.382519952


NM_014314
DDX58
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
2.333495418


NM_000311
PRNP
Prion protein (p27-30)(Creutzfeld-Jakob
2.313144503




disease, Gerstmann-Strausler-Scheinker




syndrome, fatal familial insomnia)


NM_006597
HSPA8
Heat shock 70 kDa protein 8
2.313144445


AK092040
ZCSL2
Zinc finger, CSL-type containing 2
2.303019345


NM_031483
ITCH
Itchy homolog E3 ubiquitin protein
2.300924118




ligase(mouse)


U25029
NR3C1
Nuclear receptor subfamily 3, group C,
2.273770608




member 1(glucocorticoid receptor)


NM_005242
F2RL1
Coagulation factor II (thrombin)
2.25765938




receptor-like 1


AK172851
ADORA2B
Adenosine A2b receptor
2.248876245


BQ053419
BATF
Basic leucine zipper transcription
2.220924757




factor, ATF-like


BC020698
CCL20
Chemokine(C-C motif) ligand 20
2.199037915


NM_001124
ADM
Adrenomedullin
2.186497254


NM_153609
TMPRSS6
Transmembrane protease, serine 6
2.118468174


NM_004148
NINJ1
Ninjurin 1
2.106988819


AF324224
APOL2
Apolipoprotein L, 2
2.105247356


BC040494
DNAJB2
DnaJ(Hsp40) homolog, subfamily B, member 2
2.053186934


BE512691
GPX2
Glutathione peroxidase
2.014366573




2(gastrointestinal)


NM_001002029
C4A
Complement component 4B, telomeric
2.002292152







(f) Immune response










NM_000732
CD3D
CD3D antigen, delta polypeptide(TiT3
54.93792815




complex)


AK223210
CD79B
CD79B antigen (immunoglobulin-associated
15.33023863




beta)


NM_002309
LIF
Hypothetical protein MGC20647
8.088491848


AF031469
MR1
Major histocompatibility complex, class
7.058743082




I-related


NM_172208
TAPBP
TAP binding protein (tapasin)
7.020261414


BC021117
CSF1
Colony stimulating factor 1(macrophage)
4.788017417


NM_001421
ELF4
E74-like factor 4 (ets domain transcription
4.315617246




factor)


NM_002119
HLA-DOA
Major histocompatibility complex, class
4.208814419




II, DO alpha


BX648013
TAP1
Transporter 1, ATP-binding cassette,
4.079915559




sub-family B(MDR/TAP)


AL832287
ZAP70
Zeta-chain(TCR) associated protein kinase
3.830282261




70 kDa


BF569086
NK4
Interleukin 32
3.137700229


AL832451
DKFZp451C2311
guanylate binding protein 2,
2.638811674




interferon-inducible


AF069493
SEMA7A
Sema domain, immunoglobulin domain(Ig),
2.530855422




and GPI membrane anchor, (semaphorin) 7A


NM_001008490
KLF6
Kruppel-like factor 6
2.300245668


NM_000418
IL4R
Interleukin 4 receptor
2.254471504


NM_004048
B2M
Beta-2-microglobulin
2.006239064







(g) Cell cycle










AF116720
DNAJA2
DnaJ(Hsp40) homolog, subfamily A, member 2
10.94418029


NM_001007271
DUSP13
Dual specificity phosphatase 13
7.030871577


BM904612
S100A6
S100 calcium binding protein
5.937525522




A6(calcyclin)


AK074652
SERTAD1
SERTA domain containing 1
4.83549333


NM_203394
E2F7
E2F transcription factor 7
3.647469222


NM_003620
PPM1D
Protein phosphatase 1D
3.646783838




magnesium-dependent, delta isoform


BC060797
PARD6G
Par-6 partitioning defective 6 homolog
3.501662527




gamma(C. elegans)


NM_021913
AXL
AXL receptor tyrosine kinase
3.268484061


BC040303
PTP4A1
Protein tyrosine phosphatase type IVA,
3.182840665




member 1


BC008564
PPP1R9B
Protein phosphatase 1, regulatory subunit
3.169099614




9B, spinophilin


AK097024
RAE1
RAE1 RNA export 1 homolog(S. pombe)
2.920923726


BC011957
BTG3
BTG family, member 3
2.73961999


NM_078487
CDKN2B
Cyclin-dependent kinase inhibitor 2B(p15,
2.55897751




inhibits CDK4)


BC023552
SFN
Stratifin
2.524571283


BC060847
PARD6B
Par-6 partitioning defective 6 homolog
2.507221799




beta(C. elegans)


NM_003236
TGFA
Transforming growth factor, alpha
2.487048846


NM_002632
PGF
Placental growth factor, vascular
2.435909331




endothelial growth factor-related protein


NM_015278
SASH1
SAM and SH3 domain containing 1
2.368157793


AL834276
MGC3207
Hypothetical protein MGC3207
2.364392455


AK096276
CCND3
Cyclin D3
2.291774022


AB209373
CCNK
Chromosome 14 open reading frame 65
2.27376643


NM_021132
PPP3CB
Protein phosphatase 3 (formerly 2B),
2.19575961




catalytic subunit, beta




isoform(calcineurin A beta)


NM_005072
SLC12A4
Solute carrier family
2.184117922




12(potassium/chloride transporters),




member 4


BX647886
PCNP
SPT2, Suppressor of Ty, domain containing
2.132837165




1(S. cerevisiae)


NM_044472
CDC42
Cell division cycle 42(GTP binding
2.08723035




protein, 25 kDa)


AL832973
REC8L1
REC8-like 1(yeast)
2.050105112


NM_002031
FRK
Fyn-related kinase
2.008244265







(h) Cell differentiation










BM913048
TIMP1
TIMP metallopeptidase inhibitor 1
14.51811118


NM_003975
SH2D2A
SH2 domain protein 2A
6.609387012


NM_198129
LAMA3
Laminin, alpha 3
6.313840426


NM_006152
LRMP
Lymphoid-restricted membrane protein
3.452878243


X96753
CSPG4
Chondroitin sulfate proteoglycan
3.342871665




4(melanoma-associated)


NM_000474
TWIST1
Twist homolog 1 (acrocephalosyndactyly 3;
2.962045566




Saethre-Chotzen syndrome)(Drosophila)


AF052152
DCAMKL1
Doublecortin and CaM kinase-like 1
2.946594775


AF333334
CCIN
Calicin
2.907611782


NM_005979
S100A13
S100 calcium binding protein A13
2.38786608


NM_004429
EFNB1
Ephrin-B1
2.290394007


NM_003873
NRP1
Neuropilin 1
2.102000337


AF026692
SFRP4
Secreted frizzled-related protein 4
2.097767188


AK124583
DAZAP1
DAZ associated protein 1
2.09304534


AF003837
JAG1
Jagged 1(Alagille syndrome)
2.052565745


AB209590
BMP1
Bone morphogenetic protein 1
2.018231591







(i) Cell growth










X54457
CEL
Carboxyl ester lipase (bile salt-stimulated
11.0110822




lipase)


Y11307
CYR61
Cysteine-rich, angiogenic inducer, 61
8.870510533


NM_000596
IGFBP1
Insulin-like growth factor binding
4.426373693




protein 1


NM_020997
LEFTY1
Left-right determination factor 1
4.011782013


NM_001553
IGFBP7
Insulin-like growth factor binding
3.467233091




protein 7


AB209509
IGFBP2
Insulin-like growth factor binding
2.280688594




protein 2, 36 kDa


NM_001552
IGFBP4
Insulin-like growth factor binding
2.170002081




protein 4


AB209321
CSRP2
Cysteine and glycine-rich protein 2
2.079139635


BM556279
EMP3
Epithelial membrane protein 3
2.029076597







(j) Cell proliferation










NM_005727
TSPAN1
Tetraspanin 1
8.454659213


BQ683841
S100A11
S100 calcium binding protein
6.064178076




A11(calgizzarin)


BC042390
VTI1B
Vesicle transport through interaction
4.485613104




with t-SNAREs homolog 1B(yeast)


NM_000899
KITLG
KIT ligand
3.751569954


BC032940
LAMP3
Lysosomal-associated membrane protein 3
2.728811271


BQ939577
AKR1C3
Aldo-keto reductase family 1, member
2.683290824




C3(3-alpha hydroxysteroid dehydrogenase,




type II)


BC063468
OSMR
Oncostatin M receptor
2.652841837


CR936719
SSR1
Signal sequence receptor,
2.559482267




alpha(translocon-associated protein




alpha)


AB024057
TBC1D8
TBC1 domain family, member 8(with GRAM
2.53463919




domain)


NM_003329
TXN
Thioredoxin
2.324939817


NM_012125
CHRM5
Cholinergic receptor, muscarinic 5
2.112618735


NM_007045
FGFR1OP
FGFR1 oncogene partner
2.09430482







(k) Alcohol metabolism










AK094474
RHOC
Ras homolog gene family, member C
4.227972245


BC017706
6PGL
6-phosphogluconolactonase
2.621428387


AF153821
ADH1B
Alcohol dehydrogenase IB(class I), beta
2.420867965




polypeptide


AK124656
ENO2
Enolase 2(gamma, neuronal)
2.273190922


D42047
GPD1L
Glycerol-3-phosphate dehydrogenase
2.170305169




1-like


AK130060
TALDO1
Transaldolase 1
2.107113338


AL833069
KIAA1434
Hypothetical protein KIAA1434
2.042123428


XM_496436
UNQ6077
C219-reactive peptide
2.011483091


NM_199186
BPGM
2,3-bisphosphoglycerate mutase
2.001621684







(l) DNA metabolism










U86136
TEP1
Telomerase-associated protein 1
5.354849784


BU603483
HIST1H1E
Histone 1, H1e
4.639090453


NM_005319
HIST1H1C
Histone 1, H1c
4.25715277


NM_005320
HIST1H1D
Histone 1, H1d
3.30127345


NM_003516
HIST2H2AA
Histone 2, H2aa
3.052311773


F02250
HDAC2
Histone deacetylase 2
2.559211106


NM_004739
MTA2
Metastasis associated 1 family, member 2
2.439100864


BC042589
HIST1H2BD
Histone 1, H2bd
2.361377538


CB529694
HIST2H2BE
Histone 2, H2be
2.358417445


AK126154
NP
Nucleoside phosphorylase
2.252382538


AB040899
KIAA1466
KIAA1466 gene
2.150614231


CR608156
HIST1H2AC
Histone 1, H2ac
1.894716468


BX436525
HIST1H2BH
Histone 1, H2bh
1.564280897







(m) Lipid metabolism










AK057762
TDH
L-threonine dehydrogenase
12.35436994


BC035124
UNQ2541
MSFL2541
11.28144368


BC034763
FDXR
Ferredoxin reductase
9.626878353


AK093461
ISYNA1
Myo-inositol 1-phosphate synthase A1
8.506076027


AK097009
PLTP
phospholipid transfer protein
6.838113748


AF524864
AKR1B10
Aldo-keto reductase family 1, member
5.170205623




B10(aldose reductase)


NM_021727
FADS3
Fatty acid desaturase 3
4.914325733


CR627415
HSD3B2
Hydroxy-delta-5-steroid dehydrogenase, 3
3.576157214




beta- and steroid delta-isomerase 2


CD013987
CYP21A2
Cytochrome P450, family 21, subfamily A,
3.499529399




polypeptide 2


BC020744
AKR1C4
Aldo-keto reductase family 1, member
3.073709856




C4(chlordecone reductase; 3-alpha




hydroxysteroid dehydrogenase, type I;




dihydrodiol dehydrogenase 4)


NM_004753
DHRS3
Dehydrogenase/reductase (SDR family)
2.952592956




member 3


BC030207
NR0B2
Nuclear receptor subfamily 0, group B,
2.923794075




member 2


AK091948
LTB4DH
Leukotriene B4 12-hydroxydehydrogenase
2.915047859


NM_002900
RBP3
Retinol binding protein 3, interstitial
2.882318017


BX647927
PLCD1
Phospholipase C, delta 1
2.79125248


NM_145323
OSBPL3
Oxysterol binding protein-like 3
2.683653218


NM_000169
GLA
Galactosidase, alpha
2.640805113


BC094756
CRA
Myotubularin related protein 11
2.621196547


AB043587
ACBD3
Acyl-Coenzyme A binding domain containing 3
2.387297906


NM_001478
GALGT
UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-
2.371502969




galactosylglucosylceramide




N-acetylgalactosaminyltransferase(GalNAc-




T)


J05428
UGT2B7
UDP glucuronosyltransferase 2 family,
2.29241428




polypeptide B7


AB002454
CYP4F3
Cytochrome P450, family 4, subfamily F,
2.282463636




polypeptide 3


NM_000405
GM2A
GM2 ganglioside activator
2.078054404


BC001823
PRKAB1
Protein kinase, AMP-activated, beta 1
2.069992917




non-catalytic subunit


NM_145343
APOL1
Apolipoprotein L, 1
2.065071129


NM_006571
DCTN6
Dynactin 6
2.032649122


NM_020139
DHRS6
Dehydrogenase/reductase (SDR family)
2.017235349




member 6







(n) Metal-binding










NM_000499
CYP1A1
Cytochrome P450, family 1, subfamily A,
88.34463354




polypeptide 1


BC007003
TGM4
Transglutaminase 4 (prostate)
24.06656282


NM_012189
CABYR
Calcium binding
15.7546354




tyrosine-(Y)-phosphorylation




regulated(fibrousheathin 2)


CR615530
CYGB
Cytoglobin
5.509895512


NM_004199
P4HA2
Procollagen-proline, 2-oxoglutarate
4.763879118




4-dioxygenase(proline 4-hydroxylase),




alpha polypeptide II


AK027560
CYP26A1
Cytochrome P450, family 26, subfamily A,
4.292062656




polypeptide 1


NM_145298
APOBEC3F
Apolipoprotein B mRNA editing enzyme,
4.282991592




catalytic polypeptide-like 3F


NM_005576
LOXL1
Lysyl oxidase-like 1
3.991893457


AK024854
APOBEC3B
Apolipoprotein B mRNA editing enzyme,
3.958267305




catalytic polypeptide-like 3D(putative)


NM_014301
NIFUN
NifU-like N-terminal domain containing
3.922562485


BF670653
MB
Myoglobin
3.907590399


AK126982
LHX6
LIM homeobox 6
3.753938819


Y13786
ADAM19
ADAM metallopeptidase domain 19(meltrin
3.418775374




beta)


AB051455
MICAL-L1
MICAL-like 1
3.221168592


AK074068
MICAL-L2
MICAL-like 2
3.121829223


AK124421
CPA1
Carboxypeptidase A1 (pancreatic)
3.111462293


NM_030622
CYP2S1
Cytochrome P450, family 2, subfamily S,
2.768815748




polypeptide 1


BC012027
CYP2U1
Cytochrome P450, family 2, subfamily U,
2.36896757




polypeptide 1


AY461717
ZNF385
Zinc finger protein 385
20.23244477


AK092424
ZSCAN4
Zinc finger and SCAN domain containing 4
17.62610589


AB208935
RASAL1
RAS protein activator like 1(GAP1 like)
13.22811232


NM_201444
DGKA
Diacylglycerol kinase, alpha 80 kDa
6.831831283


BC035625
EGR2
Early growth response 2(Krox-20 homolog,
5.012409534




Drosophila)


BF983391
RPS27L
Ribosomal protein S27-like
4.896252688


BC036545
PLAC2
Placenta-specific 2
4.739700637


BC034569
ZNF337
Zinc finger protein 337
4.082511332


CR936707
TRIM38
Tripartite motif-containing 38
3.756338118


AK091940
MGC4734
Ring finger protein 183
3.644058609


NM_024101
MLPH
Melanophilin
3.369249376


CR601823
HIVEP3
Human immunodeficiency virus type I
3.290777497




enhancer binding protein 3


BC041578
MGC17986
zinc finger protein 114
3.166948191


BC038432
RARA
Retinoic acid receptor, alpha
2.87080107







(o) Transport










BX641053
FXYD2
FXYD domain containing ion transport
14.84364998




regulator 2


AK095363
AQP3
Aquaporin 3
8.623096731


AK074100
SLC37A2
Solute carrier family
8.471999196




37(glycerol-3-phosphate transporter),




member 2


AK024002
TUBA4
Tubulin, alpha 4
7.427767363


AK092677
TUBB6
Tubulin, beta 6
6.458458484


NM_001297
CNGB1
Cyclic nucleotide gated channel beta 1
5.653120818


NM_032872
SYTL1
Synaptotagmin-like 1
4.930987441


NM_014045
LRP10
Low density lipoprotein receptor-related
4.616793009




protein 10


NM_014585
SLC40A1
Solute carrier family 40(iron-regulated
4.60980312




transporter), member 1


L02870
COL7A1
Collagen, type VII, alpha 1(epidermolysis
4.294284493




bullosa, dystrophic, dominant and




recessive)


AK054731
TUBA1
Tubulin, alpha 1 (testis specific)
4.193322321


AF336127
SLC4A11
Solute carrier family 4, sodium
4.116663762




bicarbonate transporter-like, member 11


AB075951
SLC22A2
Solute carrier family 22(organic cation
3.98434766




transporter), member 2


AK125026
SLC6A12
Solute carrier family 6(neurotransmitter
3.756467829




transporter, betaine/GABA), member 12


BX537541
MGC13186
Chromosome 1 open reading frame 57
3.712498811


CD013956
SHBG
Sex hormone-binding globulin
3.685973664


BC029057
DAO
D-amino-acid oxidase
3.657991139


BC041479
COL9A2
Collagen, type IX, alpha 2
3.573628765


AL050210
LOC283130
Hypothetical protein LOC283130
3.265255922


NM_004798
KIF3B
Kinesin family member 3B
3.214670618


AK025742
UCP2
Uncoupling protein 2 (mitochondrial, proton
3.187327318




carrier)


NM_021990
GABRE
Gamma-aminobutyric acid(GABA) A receptor,
3.110885736




epsilon


NM_005819
STX6
Syntaxin 6
3.089406302


BC092420
CYP4F11
Similar to Cytochrome P450
3.048152378




4F12(CYPIVF12)(UNQ568/PRO1129)


BC043423
ABCB6
ATP-binding cassette, sub-family
3.039132233




B(MDR/TAP), member 6


BC021025
CTHRC1
Collagen triple helix repeat containing 1
3.022775957


NM_004999
MYO6
Myosin VI
3.020625421


AF445025
SLC35A4
Solute carrier family 35, member A4
3.018853063


NM_003330
TXNRD1
Thioredoxin reductase 1
2.810879753


AK092512
SLC16A5
Solute carrier family 16(monocarboxylic
2.5780312




acid transporters), member 5


AB075871
FLJ42654
Golgi transport 1 homolog A(S. cerevisiae)
2.278893633







(p) The others










NM_004864
GDF15
Growth differentiation factor 15
6.654247181


NM_022153
PP2135
Chromosome 10 open reading frame 54
6.333855415


NM_001013632
LOC343521
Similar to hypothetical protein
5.344247617




4833401D15


AK124751
CAPN2
Calpain 2, (m/II) large subunit
4.877328363


AK095079
RGC32
Response gene to complement 32
3.2942944


NM_031286
SH3BGRL3
SH3 domain binding glutamic acid-rich
2.915143493




protein like 3


AK126182
KIAA1949
KIAA1949
2.807348634


NM_080388
S100A16
S100 calcium binding protein A16
2.607982075


NM_025106
SSB1
SplA/ryanodine receptor domain and SOCS
2.522542864




box containing 1


NM_203403
C9orf150
Chromosome 9 open reading frame 150
2.200130419


AK096241
SQSTM1
Sequestosome 1
2.015574807


BQ276852
TMSB10
Thymosin, beta 10
1.863965907


BC101655
HIST1H2BI
Histone 1, H2bi
1.863164701


W84524
TM4SF5
Transmembrane 4 L six family member 5
1.768210846


BX647290
HIST1H2BO
Histone 1, H2bo
1.511861325
















TABLE 3







Genes down-regulated by chrysene













Ratio of


Accession
Gene

intermediate


number
abbreviation
Gene name
value










(a) MAPKinase Signaling Pathway










NM_002467
MYC
V-myc myelocytomatosis viral oncogene
0.153957027




homolog(avian)



BC038295
MAP2K7
Mitogen-activated protein kinase kinase 7
0.250015644


NM_004364
CEBPA
CCAAT/enhancer binding protein(C/EBP),
0.291022284




alpha



NM_145161
MAP2K5
Mitogen-activated protein kinase kinase 5
0.396692291


XM_042066
MAP3K1
Mitogen-activated protein kinase kinase
0.424190161




kinase 1



Z25424
MKNK2
MAP kinase interacting serine/threonine
0.440953544




kinase 2



AB209047
ELK1
ELK1, member of ETS oncogene family
0.46931591


NM_004755
RPS6KA5
Ribosomal protein S6 kinase, 90 kDa,
0.486533941




polypeptide 5



AB001872
RPL4
Mitogen-activated protein kinase kinase
0.488828813




kinase 13



NM_002758
MAP2K6
Mitogen-activated protein kinase kinase 6
0.489648933


AK098095
BRAF
V-raf murine sarcoma viral oncogene
0.490796917




homolog B1








(b) MAPKKK cascade










AB208919
FGFR1
Fibroblast growth factor receptor
0.306072231




1(fms-related tyrosine kinase 2, Pfeiffer





syndrome)



BC060837
DUSP9
Dual specificity phosphatase 9
0.309017123


NM_000142
FGFR3
Fibroblast growth factor receptor
0.316732251




3(achondroplasia, thanatophoric dwarfism)



AY247738
TRIB3
Tribbles homolog 3 (Drosophila)
0.319013779


NM_030640
DUSP16
Dual specificity phosphatase 16
0.346335506


NM_148957
TNFRSF19
Tumor necrosis factor receptor
0.373948519




superfamily, member 19



AB011123
TNIK
TRAF2 and NCK interacting kinase
0.375429687


NM_006549
CAMKK2
Calcium/calmodulin-dependent protein
0.375436076




kinase kinase 2, beta



NM_022740
HIPK2
Homeodomain interacting protein kinase 2
0.491170791







(c) Apoptosis










NM_005400
PRKCE
Protein kinase C, epsilon
0.110495908


NM_000893
KNG1
Kininogen 1
0.123179781


NM_000066
C8B
Complement component 8, beta polypeptide
0.168259363


CR749293
PLG
Plasminogen
0.214468702


BC013780
ADORA2A
Adenosine A2a receptor
0.25462881


NM_001012271
BIRC5
Effector cell peptidase receptor 1
0.261340346


NM_003811
TNFSF9
Tumor necrosis factor (ligand)
0.278995394




superfamily, member 9



BC050369
E2F1
E2F transcription factor 1
0.284336508


NM_007313
ABL1
V-abl Abelson murine leukemia viral
0.286557271




oncogene homolog 1



BX647151
MYBL2
V-myb myeloblastosis viral oncogene
0.292335923




homolog(avian)-like 2



NM_004822
NTN1
Netrin 1
0.298168066


BC033694
BCL2L11
BCL2-like 11 (apoptosis facilitator)
0.334908167


AB208876
AXIN1
Axin 1
0.346768268


BC051332
F2
Coagulation factor II (thrombin)
0.347828025


AK122614
PPARD
Peroxisome proliferative activated
0.348064401




receptor, delta



L78790
ITGB2
Integrin, beta 2 (antigen CD18(p95),
0.377717722




lymphocyte function-associated antigen 1;





macrophage antigen 1(mac-1) beta subunit)



AK223409
BARD1
BRCA1 associated RING domain 1
0.385577392


NM_002737
PRKCA
Protein kinase C, alpha
0.38570547


NM_012479
YWHAG
Tyrosine 3-monooxygenase/tryptophan
0.38743105




5-monooxygenase activation protein, gamma





polypeptide



CR596268
ITGB3BP
Integrin beta 3 binding
0.392340911




protein(beta3-endonexin)



NM_003551
NME5
Non-metastatic cells 5, protein expressed
0.397002326




in(nucleoside-diphosphate kinase)



NM_013974
DDAH2
Dimethylarginine dimethylaminohydrolase 2
0.397010628


D79987
ESPL1
Extra spindle poles like 1(S. cerevisiae)
0.397660686


NM_006427
SIVA
CD27-binding(Siva) protein
0.399556277


NM_024900
PHF17
PHD finger protein 17
0.400845508


AB046854
MAGI-3
Membrane associated guanylate kinase, WW
0.423418754




and PDZ domain containing 3



AK095578
SPHK1
Sphingosine kinase 1
0.442516388


AK122762
CIDEA
Cell death-inducing DFFA-like effector a
0.448550433


AK092872
ERCC2
Excision repair cross-complementing
0.448557114




rodent repair deficiency, complementation





group 2 (xeroderma pigmentosum D)



NM_006290
TNFAIP3
Tumor necrosis factor, alpha-induced
0.452157996




protein 3



NM_000562
C8A
Complement component 8, alpha polypeptide
0.463461593


AK122894
AKT1
V-akt murine thymoma viral oncogene
0.467334939




homolog 1



NM_005067
SIAH2
Seven in absentia homolog 2(Drosophila) 
0.467840694


NM_203339
CLU
Clusterin(complement lysis inhibitor,
0.469884724




SP-40,40, sulfated glycoprotein 2,





testosterone-repressed prostate message





2, apolipoprotein J)



NM_003376
VEGF
Vascular endothelial growth factor
0.481416264


AK027590
DAPK3
Death-associated protein kinase 3
0.48390449


AK122825
HMGB1
High-mobility group box 1
0.505043422







(d) Response to DNA damage stimulus










NM_006904
PRKDC
Protein kinase, DNA-activated, catalytic
0.136872821




polypeptide



AK074377
UHRF1
Chromosome 19 open reading frame 31
0.162554272


CR604810
CCNA2
Cyclin A2
0.271739971


AK001720
FLJ10858
Nei endonuclease VIII-like 3(E. coli)
0.272320034


CR600021
HMGB2
High-mobility group box 2
0.292500615


NM_006230
POLD2
Polymerase(DNA directed), delta 2,
0.296598073




regulatory subunit 50 kDa



NM_018151
RIF1
RAP1 interacting factor homolog(yeast)
0.337901829


BX248766
RAD51L1
RAD51-like 1(S. cerevisiae)
0.35239904


NM_014502
PRP19
PRP19/PSO4 pre-mRNA processing factor 19
0.352412499




homolog(S. cerevisiae)



NM_013975
LIG3
Ligase III, DNA, ATP-dependent
0.366235708


NM_173627
FLJ35220
Hypothetical protein FLJ35220
0.370187726


NM_003362
UNG
Uracil-DNA glycosylase
0.378408018


AF076494
IRF7
Interferon regulatory factor 7
0.385612995


CR625391
RAD51AP1
RAD51 associated protein 1
0.391222632


NM_006231
POLE
Polymerase(DNA directed), epsilon
0.394826988


NM_130398
EXO1
Exonuclease 1
0.407524268


DQ140356
KIAA1596
Fanconi anemia, complementation group M
0.412353616


NM_002105
H2AFX
H2A histone family, member X
0.41455303


AB209560
POLD1
Polymerase(DNA directed), delta 1,
0.443656469




catalytic subunit 125 kDa



NM_000136
FANCC
Fanconi anemia, complementation group C
0.444998791


NM_003368
USP1
Ubiquitin specific peptidase 1
0.445903079


NM_022836
DCLRE1B
DNA cross-link repair 1B(PSO2 homolog, S. cerevisiae)
0.446832858


NM_001067
TOP2A
Topoisomerase(DNA) II alpha 170 kDa
0.450120675


NM_002913
RFC1
Replication factor C(activator1) 1, 145 kDa
0.471937101


NM_002412
MGMT
O-6-methylguanine-DNA methyltransferase
0.492152777


NM_000251
MSH2
MutS homolog2, colon cancer, nonpolyposis
0.582053356




type 1(E. coli)








(e) Immune response










NM_015254
KIF13B
Kinesin family member 13B
0.20677975


NM_006037
HDAC4
Histone deacetylase 4
0.233288586


NM_020995
HPR
Haptoglobin-related protein
0.242867738


NM_153443
KIR3DL1
Killer cell immunoglobulin-like
0.281426014




receptor, three domains, long cytoplasmic





tail, 1



BC071718
CCL4L1
Chemokine(C-C motif) ligand 4-like 1
0.294604522


NM_020979
APS
Adaptor protein with pleckstrin homology
0.29487541




and src homology 2 domains



NM_001629
ALOX5AP
Arachidonate 5-lipoxygenase-activating
0.300419669




protein



NM_130852
PLUNC
Palate, lung and nasal epithelium
0.320441751




carcinoma associated



AK024499
SPON2
Spondin 2, extracellular matrix protein
0.328221638


BC048198
AHSG
Alpha-2-HS-glycoprotein
0.344058212


AK095849
RNU22
RNA, U22 small nucleolar
0.353888139


BC005395
HPX
Hemopexin
0.367891973


NM_012276
ILT7
Leukocyte immunoglobulin-like receptor,
0.392427034




subfamily A(with TM domain), member 4



NM_003152
STAT5A
Signal transducer and activator of
0.400291045




transcription 5A



BC037171
CCL27
Chemokine(C-C motif) ligand 27
0.40075022


NM_002468
MYD88
Myeloid differentiation primary response
0.403137375




gene(88)



NM_002215
ITIH1
Inter-alpha (globulin) inhibitor H1
0.405944172


NM_004750
CRLF1
Cytokine receptor-like factor 1
0.413787497


DR004094
HAMP
Hepcidin antimicrobial peptide
0.413938464


BC063245
GNLY
Granulysin
0.422712087


AJ290445
SARM1
Sterile alpha and TIR motif containing 1
0.430080233


AK122686
IF
I factor(complement)
0.436309666


AB008535
GPR44
G protein-coupled receptor 44
0.440552612


AK122912
ZGPAT
Zinc finger, CCCH-type with G patch domain
0.44067651


BC022312
C4BPA
Complement component 4 binding protein,
0.442950052




alpha



BG564683
ORM1
Orosomucoid 1
0.445506981


NM_000625
NOS2A
Nitric oxide synthase 2A(inducible,
0.460550527




hepatocytes)



NM_005515
HLXB9
Homeo box HB9
0.467040193


BX537504
C2
Complement component 2
0.484853058


BC028153
LILRB3
Leukocyte immunoglobulin-like receptor,
0.490534341




subfamily B(with TM and ITIM domains),





member 3



AF130100
SERPINC1
Serpin peptidase inhibitor, clade
0.491818077




C(antithrombin), member 1



BC015110
HHEX
Hematopoietically expressed homeobox
0.602852309







(f) Mitotic cell cycle










AB209179
PLK1
Polo-like kinase 1 (Drosophila) 
0.120946155


AY367065
ASPM
Asp(abnormal spindle)-like,
0.186885667




microcephaly associated(Drosophila) 



NM_006101
KNTC2
Kinetochore associated 2
0.257114298


NM_003550
MAD1L1
MAD1 mitotic arrest deficient-like
0.268688634




1(yeast)



NM_020242
KNSL7
Kinesin family member 15
0.280736071


AB033068
FZR1
Fizzy/cell division cycle 20 related
0.281781782




1(Drosophila) 



NM_018492
PBK
PDZ binding kinase
0.284074566


NM_003318
TTK
TTK protein kinase
0.3026269


BC011000
CDCA5
Cell division cycle associated 5
0.31212201


NM_003981
PRC1
Protein regulator of cytokinesis 1
0.313872555


NM_015097
CLASP2
Cytoplasmic linker associated protein 2
0.336054596


U65410
MAD2L1
MAD2 mitotic arrest deficient-like
0.34214776




1(yeast)



NM_016343
CENPF
Centromere protein F, 350/400 ka(mitosin)
0.344911485


NM_005504
BCAT1
Branched chain aminotransferase 1,
0.345858236




cytosolic



NM_003718
CDC2L5
Cell division cycle 2-like
0.354681271




5(cholinesterase-related cell division





controller)



NM_002768
PCOLN3
Procollagen(type III) N-endopeptidase
0.389132906


BC004352
KIF22
Kinesin family member 22
0.391492074


NM_005983
SKP2
S-phase kinase-associated protein 2(p45)
0.39153455


NM_004523
KIF11
Kinesin family member 11
0.421587881


CR592757
BRRN1
Barren homolog 1 (Drosophila) 
0.430159766


NM_019063
EML4
Echinoderm microtubule associated
0.434736842




protein like 4



NM_006845
KIF2C
Kinesin family member 2C
0.43971662


NM_004701
CCNB2
Cyclin B2
0.442694391


NM_138555
KIF23
Kinesin family member 23
0.449014129


NM_182687
PKMYT1
Protein kinase, membrane associated
0.452437142




tyrosine/threonine 1



NM_001790
CDC25C
Cell division cycle 25C
0.460379159


BC032677
UBE2C
Ubiquitin-conjugating enzyme E2C
0.462505602


NM_005886
KATNB1
Katanin p80(WD repeat containing) subunit
0.464765241




B1



BC073878
KIFC1
Kinesin family member C1
0.481322258


AL833191
SMC2L1
SMC2 structural maintenance of
0.486060683




chromosomes 2-like 1(yeast)



AF053305
BUB1
BUB1 budding uninhibited by
0.491236752




benzimidazoles 1 homolog(yeast)



Z25431
NEK1
NIMA(never in mitosis gene a)-related
0.49168642




kinase 1



BC043371
GFI1B
Growth factor independent 1B(potential
0.494495314




regulator of CDKN1A, translocated in CML)



CR933728
CDC2
Cell division cycle 2, G1 to S and G2 to M
0.496459108


NM_014750
DLG7
Discs, large homolog 7(Drosophila) 
0.580070458







(g) Cell cycle










NM_018560
WWOX
WW domain containing oxidoreductase
0.079825512


BG203702
FHIT
Nasopharyngeal carcinoma,
0.093083555




down-regulated 1



BC050384
SUPT3H
Suppressor of Ty 3 homolog(S. cerevisiae)
0.126706068


NM_000189
HK2
Hexokinase 2
0.173385257


CD049340
AURKB
Aurora kinase B
0.19031938


AB011153
PLCB1
Phospholipase C, beta
0.215552494




1(phosphoinositide-specific)



AB209271
ERF
Ets2 repressor factor
0.232018189


NM_014264
PLK4
Polo-like kinase 4 (Drosophila) 
0.247388103


NM_002417
MKI67
Antigen identified by monoclonal antibody
0.258796008




Ki-67



XM_496557
RGP1
Plasminogen-like B2
0.294326139


NM_170715
RASSF1
Ras association (RalGDS/AF-6) domain
0.298232376




family 1



NM_004526
MCM2
MCM2 minichromosome maintenance
0.302992712




deficient 2, mitotin(S. cerevisiae)



BC033086
TCF19
Transcription factor 19(SC1)
0.32373718


NM_001007071
RPS6KB2
Ribosomal protein S6 kinase, 70 kDa,
0.324307585




polypeptide 2



NM_016272
TOB2
Transducer of ERBB2, 2
0.330193495


AK126688
CDYL2
Chromodomain protein, Y-like 2
0.387113875


NM_001759
CCND2
Cyclin D2
0.389524403


NM_002388
MCM3
MCM3 minichromosome maintenance
0.398257469




deficient 3(S. cerevisiae)



NM_182776
MCM7
MCM7 minichromosome maintenance
0.404714589




deficient 7(S. cerevisiae)



NM_012145
DTYMK
Deoxythymidylate kinase(thymidylate
0.423208336




kinase)



NM_014751
MTSS1
Metastasis suppressor 1
0.43748772


NM_024342
GRLF1
Glucocorticoid receptor DNA binding
0.442555888




factor 1



AK025627
CABLES1
Cdk5 and Abl enzyme substrate 1
0.471323938


NM_005915
MCM6
MCM6 minichromosome maintenance
0.472089288




deficient 6(MIS5 homolog, S. pombe) (S. cerevisiae)



AF196185
PARD3
Par-3 partitioning defective 3 homolog(C. elegans)
0.484933312


NM_019001
XRN1
5′-3′ exoribonuclease 1
0.488883063


NM_006739
MCM5
MCM5 minichromosome maintenance
0.491549947




deficient 5, cell division cycle 46(S. cerevisiae)



NM_012218
ILF3
Interleukin enhancer binding factor 3,
0.492503876




90 kDa



AB053172
CDT1
DNA replication factor
0.493969454


AK074112
CCNL2
Cyclin L2
0.498469976


NM_001005414
ZWINT
ZW10 interactor antisense
0.584517014







(h) Alcohol metabolism










BC033391
PP1665
Glycerophosphodiester phosphodiesterase
0.215800503




domain containing 5



NM_000672
ADH6
Alcohol dehydrogenase 6(class V)
0.271123842


NM_004563
PCK2
Phosphoenolpyruvate carboxykinase
0.337047159




2(mitochondrial)



XM_113962
KIAA0650
Structural maintenance of chromosomes
0.348581608




flexible hinge domain containing 1



NM_001500
GMDS
GDP-mannose 4,6-dehydratase
0.354747755


NM_002541
OGDH
Oxoglutarate (alpha-ketoglutarate)
0.357289949




dehydrogenase(lipoamide)



NM_000298
PKLR
Pyruvate kinase, liver and RBC
0.39231759


NM_005276
GPD1
Glycerol-3-phosphate dehydrogenase
0.394511398




1(soluble)



AB209127
PFKL
Phosphofructokinase, liver
0.396666891


M15943
ADH4
Alcohol dehydrogenase 4(class II), pi
0.441308466




polypeptide



NM_000671
ADH5
Alcohol dehydrogenase 5(class III), chi
0.448054959




polypeptide



BC026320
OGDHL
Oxoglutarate dehydrogenase-like
0.464556343


NM_009590
AOC2
Amine oxidase, copper containing
0.479506902




2(retina-specific)



BC015797
SLC25A10
Solute carrier family 25(mitochondrial
0.489192347




carrier; dicarboxylate transporter),





member 10








(i) Amino acid metabolism










NM_003748
ALDH4A1
Aldehyde dehydrogenase 4 family, member A1
0.179005599


NM_002108
HAL
Histidine ammonia-lyase
0.19694868


NM_003486
SLC7A5
Solute carrier family 7(cationic amino
0.201120723




acid transporter, y+ system), member 5



NM_003045
SLC7A1
Solute carrier family 7(cationic amino
0.204215808




acid transporter, y+ system), member 1



NM_000277
PAH
Phenylalanine hydroxylase
0.207720611


NM_012331
MSRA
Methionine sulfoxide reductase A
0.21152792


BC008366
DDC
Dopa decarboxylase (aromatic L-amino acid
0.219970928




decarboxylase)



NM_006567
FARS2
Phenylalanine-tRNA synthetase
0.242735735




2(mitochondrial)



AK055862
FLJ31300
Urocanase domain containing 1
0.265306391


NM_001875
CPS1
Carbamoyl-phosphate synthetase 1,
0.284603831




mitochondrial



AK122685
GLUD1
Glutamate dehydrogenase 1
0.290594908


NM_003046
SLC7A2
Solute carrier family 7(cationic amino
0.303490377




acid transporter, y+ system), member 2



AK093306
PHGDH
Phosphoglycerate dehydrogenase
0.315789771


NM_080820
HARS2
Histidyl-tRNA synthetase 2
0.337699715


NM_006907
PYCR1
Pyrroline-5-carboxylate reductase 1
0.343360051


NM_020117
LARS
Leucyl-tRNA synthetase
0.359457546


BC051726
GLUL
Glutamate-ammonia ligase(glutamine
0.363765571




synthetase)



BC012616
BHMT
Betaine-homocysteine methyltransferase
0.369989797


AK055053
SHMT2
Serine hydroxymethyltransferase
0.401235464




2(mitochondrial)



NM_007101
SARDH
Sarcosine dehydrogenase
0.402864636


AL833251
LOC283970
Hypothetical protein LOC283970
0.40730291


AK027126
ASS
Argininosuccinate synthetase
0.418690738


L00972
CBS
Cystathionine-beta-synthase
0.440354778


NM_206965
FTCD
Formiminotransferase cyclodeaminase
0.47079397


NM_000429
MAT1A
Methionine adenosyltransferase I, alpha
0.472559543


NM_001801
CDO1
Cysteine dioxygenase, type I
0.480848737


NM_006843
SDS
Serine dehydratase
0.488304529


NM_000170
GLDC
Glycine dehydrogenase (decarboxylating;
0.491686799




glycine decarboxylase, glycine cleavage





system protein P)








(j) DNA metabolism










BX102654
HIST1H4B
Histone 1, H4b
0.172360533


DQ097177
UREB1
HECT, UBA and WWE domain containing 1
0.213344474


NM_022743
SMYD3
SET and MYND domain containing 3
0.265102932


NM_001809
CENPA
Centromere protein A, 17 kDa
0.272224677


AB028450
NCOR1
Nuclear receptor co-repressor 1
0.31777205


NM_002967
SAFB
Scaffold attachment factor B
0.3219512


NM_003074
SMARCC1
SWI/SNF related, matrix associated, actin
0.353538227




dependent regulator of chromatin,





subfamily c, member 1



NM_015409
EP400
E1A binding protein p400
0.371543731


BC044659
SMARCA3
SWI/SNF related, matrix associated, actin
0.393689415




dependent regulator of chromatin,





subfamily a, member 3



AB209433
BAT8
Euchromatic histone-lysine
0.398851055




N-methyltransferase 2



XM_370756
KIAA1305
KIAA1305
0.41059832


NM_020201
NT5M
5′,3′-nucleotidase, mitochondrial
0.425450039


NM_001025248
DUT
DUTP pyrophosphatase
0.4301363


AK123010
RRM2
Ribonucleotide reductase M2 polypeptide
0.443733966


BX537961
DNMT2
DNA (cytosine-5-)-methyltransferase 2
0.449895729


BX648935
TBL1XR1
Transducin (beta)-like 1X-linked receptor 1
0.45144224


NM_001001888
VCX-C
Variably charged X-C
0.451813598


NM_002501
NFIX
Nuclear factor I/X (CCAAT-binding
0.455473267




transcription factor)



NM_003483
HMGA2
High mobility group AT-hook 2
0.45812854


U80628
TK2
Thymidine kinase 2, mitochondrial
0.460005269


NM_006807
CBX1
Chromobox homolog 1 (HP1 beta homolog
0.46081477




Drosophila)



AY024361
MLL3
Myeloid/lymphoid or mixed-lineage
0.461050924




leukemia 3



NM_017519
ARID1B
AT rich interactive domain 1B(SWI1-like)
0.46115551


NM_003077
SMARCD2
SWI/SNF related, matrix associated, actin
0.461333882




dependent regulator of chromatin,





subfamily d, member 2



NM_016937
POLA
Polymerase(DNA directed), alpha
0.463508595







(k) Lipid metabolism










AJ007583
LARGE
Like-glycosyltransferase
0.142759166


AK124635
PCSK9
Proprotein convertase subtilisin/kexin
0.146781198




type 9



NM_030758
OSBP2
Oxysterol binding protein 2
0.148618668


NM_005063
SCD
Stearoyl-CoA
0.16572839




desaturase(delta-9-desaturase)



NM_000236
LIPC
Lipase, hepatic
0.198492731


AF288389
GLT25D2
Glycosyltransferase 25 domain containing 2
0.198600514


AF202889
APOA5
Apolipoprotein A-V
0.209911653


BX640945
FADS2
Fatty acid desaturase 2
0.213145374


BI521580
APOC3
Apolipoprotein C-III
0.23320201


NM_001277
CHKA
Choline kinase alpha
0.244381018


Z28339
AKR1D1
Aldo-keto reductase family 1, member
0.254902416




D1(delta





4-3-ketosteroid-5-beta-reductase)



BC035654
NR1H4
Nuclear receptor subfamily 1, group H,
0.262741172




member 4



AB209229
MVD
Mevalonate (diphospho) decarboxylase
0.269846903


BC026264
UGT2B4
UDP glucuronosyltransferase 2 family,
0.271647964




polypeptide B4



NM_004104
FASN
Fatty acid synthase
0.280448461


NM_147161
THEA
Acyl-CoA thioesterase 11
0.285998121


AF059203
SOAT2
Sterol O-acyltransferase 2
0.288070163


NM_020918
GPAM
Glycerol-3-phosphate acyltransferase,
0.292909604




mitochondrial



NM_001443
FABP1
Fatty acid binding protein 1, liver
0.296451689


NM_014762
DHCR24
24-dehydrocholesterol reductase
0.297972079


AK093328
PCYT2
Phosphate cytidylyltransferase 2,
0.311874518




ethanolamine



BX538214
C6orf167
Chromosome 6 open reading frame 167
0.323543551


X75311
MVK
Mevalonate kinase (mevalonic aciduria)
0.333966231


NM_000497
CYP11B1
Cytochrome P450, family 11, subfamily B,
0.338436304




polypeptide 1



BC036102
SIAT7F
ST6(alpha-N-acetyl-neuraminyl-2,3-beta-
0.34576685




galactosyl-1,3)-N-acetylgalactosaminide





alpha-2,6-sialyltransferase 6



BC012172
ACAS2
Acyl-CoA synthetase short-chain family
0.350569281




member 2



NM_007169
PEMT
Phosphatidylethanolamine
0.356048412




N-methyltransferase



X91148
MTP
Microsomal triglyceride transfer protein
0.362753622


NM_198839
ACACA
Acetyl-Coenzyme A carboxylase alpha
0.380784496


NM_004687
MTMR4
Myotubularin related protein 4
0.401837935


NM_000384
APOB
Apolipoprotein B (including Ag(x) antigen)
0.416233282


AK127051
ACAA1
Acetyl-Coenzyme A acyltransferase
0.423299321




1(peroxisomal 3-oxoacyl-Coenzyme A





thiolase)



BC035638
LSS
Lanosterol synthase
0.430665775




(2,3-oxidosqualene-lanosterol cyclase)



NM_004204
PIGQ
Phosphatidylinositol glycan, class Q
0.45218399


NM_004457
ACSL3
Acyl-CoA synthetase long-chain family
0.454938664




member 3



AL138578
ADPN
Adiponutrin
0.461837251


NM_002332
LRP1
Low density lipoprotein-related protein
0.468691544




1(alpha-2-macroglobulin receptor)



NM_016372
GPR175
G protein-coupled receptor 175
0.479022743


NM_080476
CDC91L1
CDC91 cell division cycle 91-like 1(S. cerevisiae)
0.479354103


AF303134
ALDH8A1
Aldehyde dehydrogenase 8 family, member A1
0.481811965


NM_002130
HMGCS1
3-hydroxy-3-methylglutaryl-Coenzyme A
0.483678121




synthase 1(soluble)



AK055414
LASS4
LAG1 longevity assurance homolog 4(S. cerevisiae)
0.485211697


NM_022977
ACSL4
Acyl-CoA synthetase long-chain family
0.485591269




member 4



NM_004631
LRP8
Low density lipoprotein receptor-related
0.490795611




protein 8, apolipoprotein e receptor



NM_002573
PAFAH1B3
Platelet-activating factor
0.495601801




acetylhydrolase, isoform Ib, gamma subunit





29 kDa



NM_021005
NR2F2
Nuclear receptor subfamily 2, group F,
0.497299707




member 2








(l) Metal-binding










CR620135
DPEP1
Dipeptidase 1(renal)
0.190430174


NM_002317
LOX
Lysyl oxidase
0.198239785


NM_005949
MT1F
Metallothionein 1F (functional)
0.22405551


AF279145
ANTXR1
Anthrax toxin receptor 1
0.230840625


NM_005588
MEP1A
Meprin A, alpha(PABA peptide hydrolase)
0.259485084


NM_014214
IMPA2
Inositol(myo)-1(or 4)-monophosphatase 2
0.263186701


NM_005940
MMP11
Matrix metallopeptidase 11(stromelysin
0.264566247




3)



AB193259
ACE2
Angiotensin I converting
0.273758292




enzyme(peptidyl-dipeptidase A) 2



AF018081
COL18A1
Collagen, type XVIII, alpha 1
0.291828069


M28016
cytochrome b
Human mitochondrial cytochrome b gene,
0.297761706




partial cds.



NM_002428
MMP15
Matrix metallopeptidase
0.31207988




15(membrane-inserted)



NM_025074
FRAS1
Fraser syndrome 1
0.33237663


M75106
CPB2
Carboxypeptidase B2 (plasma,
0.614512721




carboxypeptidase U)



NM_000937
POLR2A
Polymerase(RNA) II (DNA directed)
0.204681289




polypeptide A, 220 kDa



NM_005060
RORC
RAR-related orphan receptor C
0.221567885


BC092512
MYRIP
Myosin VIIA and Rab interacting protein
0.248675106


NM_000901
NR3C2
Nuclear receptor subfamily 3, group C,
0.266066448




member 2



NM_005392
PHF2
PHD finger protein 2
0.268831722


AK091289
ZNF367
Zinc finger protein 367
0.275012045


NM_014788
TRIM14
Tripartite motif-containing 14
0.276556442


NM_018660
ZNF395
Zinc finger protein 395
0.296616246


AB209755
MLLT10
Myeloid/lymphoid or mixed-lineage
0.300538557




leukemia(trithorax homolog, Drosophila);





translocated to, 10



AK097472
MAZ
MYC-associated zinc finger
0.313722345




protein(purine-binding transcription





factor)



NM_020062
SLC2A4RG
SLC2A4 regulator
0.33059257


BX571750
HNF4G
Hepatocyte nuclear factor 4, gamma
0.332470234







(m) Transport










NM_021807
SEC8L1
Exocyst complex component 4
0.089545841


NM_006651
CPLX1
Complexin 1
0.131109907


NM_005845
ABCC4
ATP-binding cassette, sub-family
0.133334881




C(CFTR/MRP), member 4



NM_006650
CPLX2
Complexin 2
0.134012051


NM_004164
RBP2
Retinol binding protein 2, cellular
0.156673775


NM_021069
ARGBP2
Sorbin and SH3 domain containing 2
0.166678886


NM_201649
SLC6A9
Solute carrier family 6(neurotransmitter
0.214191554




transporter, glycine), member 9



NM_005835
SLC17A2
Solute carrier family 17(sodium
0.221982855




phosphate), member 2



AJ315644
SLC2A13
Solute carrier family 2(facilitated
0.222684082




glucose transporter), member 13



NM_000340
SLC2A2
Solute carrier family 2(facilitated
0.227123291




glucose transporter), member 2



NM_003685
KHSRP
KH-type splicing regulatory protein(FUSE
0.22945097




binding protein 2)



BX537382
SLC38A3
Solute carrier family 38, member 3
0.242568721


NM_182964
NAV2
Neuron navigator 2
0.247250994


BX640965
SLC22A3
Solute carrier family 22(extraneuronal
0.255608537




monoamine transporter), member 3



AK127255
SNX26
Sorting nexin 26
0.260176313


AY043484
SCN1A
Sodium channel, voltage-gated, type I,
0.260866507




alpha



AK074107
C20orf59
Chromosome 20 open reading frame 59
0.271346073


NM_003056
SLC19A1
Solute carrier family 19(folate
0.29688016




transporter), member 1



NM_020897
HCN3
Hyperpolarization activated cyclic
0.313835834




nucleotide-gated potassium channel 3



AY124771
SLC26A1
Solute carrier family 26(sulfate
0.317100952




transporter), member 1



NM_001011554
SLC13A3
Solute carrier family
0.321469735




13(sodium-dependent dicarboxylate





transporter), member 3



AK027855
SLC5A6
Solute carrier family 5(sodium-dependent
0.327464039




vitamin transporter), member 6



NM_001005747
CACNB4
Calcium channel, voltage-dependent, beta
0.332952777




4 subunit



NM_013277
RACGAP1
Rac GTPase activating protein 1
0.378143166







(n) The others










NM_012340
NFATC2
Nuclear factor of activated T-cells,
0.417449456




cytoplasmic, calcineurin-dependent 2



NM_018291
FLJ10986
Hypothetical protein FLJ10986
0.181870965


BC052951
LMNB1
Lamin B1
0.197126008


AK075271
SPTLC2L
Serine palmitoyltransferase, long chain
0.230098147




base subunit 2-like(aminotransferase 2)



BI762502
GSTA2
Glutathione S-transferase A2
0.31916011


AK055221
FBXO5
F-box protein 5
0.342113931


XM_372695
LOC390859
PREDICTED: Homo sapiens similar to Chain
0.356013687




A, Crystal Structure Of The R463a Mutant





Of Human Glutamate





Dehydrogenase(LOC390859), mRNA



AB037805
KLHL14
Kelch-like 14 (Drosophila) 
0.376251676


NM_145061
C13orf3
Chromosome 13 open reading frame 3
0.377419925


AK000643
ASB9
Ankyrin repeat and SOCS box-containing 9
0.419775956


AL133031
MLR1
Transcription factor MLR1
0.438551394


AB091343
C10orf3
Centrosomal protein 55 kDa
0.451690933


AY007723
MAL2
Mal, T-cell differentiation protein 2
0.456188931


BC098313
FLJ20641
Hypothetical protein FLJ20641
0.508085568


BC041882
ATF7IP2
Activating transcription factor 7
0.521200373




interacting protein 2



AK127098
FLJ10706
Chromosome 1 open reading frame 112
0.527226934


BC022255
Spc25
Spindle pole body component 25 homolog(S. cerevisiae)
0.537391579


BC026283
APOH
Apolipoprotein H (beta-2-glycoprotein I)
0.650367241









Example 3
Quantification by Real Time RT-PCR (Reverse Transcriptase Polymerase Chain Reaction)
Among genes apt to be more than 2 fold up-regulated or down-regulated by chrysene, confirmed in Example 2, 25 genes up-regulated and 10 genes down-regulated were selected. The genes are Genebank Accession Number: NM—000499 (Cytochrome P450, family 1, subfamily A, polypeptide 1), Genebank Accession Number: BX649164 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1), Genebank Accession Number: NM—004881 (Tumor protein p53 inducible protein 3), Genebank Accession Number: BM913048 (TIMP metallopeptidase inhibitor 1), Genebank Accession Number: NM—006426 (Dihydropyrimidinase-like 4), Genebank Accession Number: NM—133493 [CD109 antigen (Gov platelet alloantigens)], Genebank Accession Number: AB033073 (Sulfatase 2), Genebank Accession Number: NM—021603 (FXYD domain containing ion transport regulator 2), Genebank Accession Number: BC035124 (MSFL2541), Genebank Accession Number: Y11307 (Cysteine-rich, angiogenic inducer, 61), Genebank Accession Number: AK095363 (Aquaporin 3), Genebank Accession Number: AK097009 (phospholipid transfer protein), Genebank Accession Number: NM—004864 (Growth differentiation factor 15), Genebank Accession Number: BU587578 (Regulator of G-protein signaling 10), Genebank Accession Number: BQ683841 [S100 calcium binding protein A11 (calgizzarin)], Genebank Accession Number: NM—001013632 [polo-like kinase 3 (Drosophila)], Genebank Accession Number: NM—032872 (Synaptotagmin-like 1), Genebank Accession Number: BC042390 [Vesicle transport through interaction with t-SNAREs homolog 1B (yeast)], Genebank Accession Number: NM—000596 (Insulin-like growth factor binding protein 1), Genebank Accession Number: NM—005319 (Histone 1, H1c), Genebank Accession Number: AK094474 (Ras homolog gene family, member C), Genebank Accession Number: NM—001769 [CD9 antigen (p24)], Genebank Accession Number: NM—001553 (Insulin-like growth factor binding protein 7), Genebank Accession Number: NM—003897 (Immediate early response 3), Genebank Accession Number: NM—003516 (Histone 2, H2aa), Genebank Accession Number: AK074377 (Chromosome 19 open reading frame 31), Genebank Accession Number: BX640965 (Solute carrier family 22 (extraneuronal monoamine transporter), member 3), Genebank Accession Number: NM—018492 (PDZ binding kinase), Genebank Accession Number: CR600021 (High-mobility group box 2), Genebank Accession Number: NM—006101 (Kinetochore associated 2), Genebank Accession Number: NM—003318 (TTK protein kinase), Genebank Accession Number: U65410 [MAD2 mitotic arrest deficient-like 1 (yeast)], Genebank Accession Number: NM—001067 (Topoisomerase (DNA) II alpha 170 kDa), Genebank Accession Number: AB091343 (Centrosomal protein 55 kDa) and Genebank Accession Number: BC022255 [Spindle pole body component 25 homolog (S. cerevisiae)].
To investigate and quantify the expressions of those genes, primers which can specifically proliferate of those genes' mRNA (see Table 4) were prepared, and quantitative real time RT-PCR was performed using My IQ Real-time PCR (Bio-rad, USA). Particularly, reverse transcription was performed using oligo dT primer and Superscript kit (OmniscriptTM kit, Qiagen, Colo., USA) to synthesize cDNA. 0.2 μl of the cDNA, 3.8 μl of water, 0.5 μl of sense primer, 0.5 μl of antisense primer, and 5 μl of SYBR Green I staining supermix (Bio-rad, USA) were mixed, and the mixture was placed in PCR tube, followed by RT-PCR in My IQ real time PCR machine as follows: step 1, at 95° C. for 3 minutes; step 2 (repeated 45 times), step 2-1, at 95° C. for 10 seconds, step 2-2, at 55-65° C. for 45 seconds; step 3, at 95° C. for 1 minute; step 4 at 55° C. for 1 minute; and step 5 (repeated 80 times) at 55° C. for 10 seconds. To quantify PCR product, SYBR Green I (Bio-rad, USA) staining was performed. SYBR Green I staining is based on binding to double stranded DNA, so that as double stranded DNA increases during PCR, fluorescence intensity increases. The target gene used for PCR and primers for endogenous GAPDH were added to SYBR Green master mix, followed by PCR. Then, primer optimization was performed to select optimum concentration. The synthesized cDNA was mixed with each primer (Table 4), to which the SYBR Green master mix was added, followed by PCR. Quantification was performed using quantitative software (see Table 5).







TABLE 4







Primer sequences










Accession





number
Gene name
PCR primer sequence (5′→3′)














NM_000499
Cytochrome P450,
sense
CACCATCCCCCACAGCAC




family 1, subfamily A,
(SEQ. ID. NO: 1)



polypeptide 1


 




antisense
ACAAAGACACAACGCCCCTT




(SEQ. ID. NO: 2)


 


BX649164
Serpin peptidase
sense
CAGACCAAGAGCCTCTCCAC



inhibitor, clade
(SEQ. ID. NO: 3)



E (nexin, plasminogen



activator inhibitor



type 1), member 1


 




antisense
ATCACTTGGCCCATGAAAAG




(SEQ. ID. NO: 4)


 


NM_004881
Tumor protein p53
sense
AGGGTGAAGTCCTCCTGAAGGT



inducible protein 3
(SEQ. ID. NO: 5)


 




antisense
GTGGGTCATACTGGCCTTGTCT




(SEQ. ID. NO: 6)


 


BM913048
TIMPmetallopeptidase
sense
GATACTTCCACAGGTCCCACAAC



inhibitor1
(SEQ. ID. NO: 7)


 




antisense
GCAAGAGTCCATCCTGCAGTT




(SEQ. ID. NO: 8)


 


NM_006426
Dihydropyrimidinase-
sense
TGGGAAGATGGACGAGAATGAGT



like4
(SEQ. ID. NO: 9)
TCG


 




antisense
ACTCCACTCCCTCGAAGATGTTGT




(SEQ. ID. NO: 10)


 


NM_133493
CD109 antigen (Gov
sense
GCCAAGATTTACCACGTTTGCCCA



platelet
(SEQ. ID. NO: 11)



alloantigens)


 




antisense
TGAAGGACCAGAACTGAGCCACAT




(SEQ. ID. NO: 12)


 


AB033073
Sulfatase 2
sense
TGCGGATATGGACGGGAAATCCAT




(SEQ. ID. NO: 13)


 




antisense
TCTCTTGTGTAGCAGCTTGCCTCT




(SEQ. ID. NO: 14)


 


NM_021603
FXYDdomain containing
sense
GGCAATAAGAAGCGCAGGCAAATC



ion transport
(SEQ. ID. NO: 15)



regulator 2


 




antisense
AAGGTCTAAAGCCCAGGGAAGAAG




(SEQ. ID. NO: 16)


 


BC035124
MSFL2541
sense
TTCGCCGTCCTTTACATCTAC




(SEQ. ID. NO: 17)


 




antisense
AGAGCCTGGGGACTCACAT




(SEQ. ID. NO: 18)


 


Y11307
Cysteine-rich,
sense
ATTGTAGAAAGGAAGCCTTGCTCAT



angiogenic inducer, 61
(SEQ. ID. NO: 19)


 




antisense
TCCAATCGTGGCTGCATTAG




(SEQ. ID. NO: 20)


 


AK095363
Aquaporin 3
sense
TTCACGATCCACCCTTTCAGGCTA




(SEQ. ID. NO: 21)


 




antisense
ACACATACCTGCTGCCCATTCTCT




(SEQ. ID. NO: 22)


 


AK097009
Phospholipid transfer
sense
TGCCACAGAGAAGACGGGATTTGA



protein
(SEQ. ID. NO: 23)


 




antisense
AGGTGGTGGACGGACTGTAATTGA




(SEQ. ID. NO: 24)


 


NM_004864
Growth
sense
CCTGAGACACCCGATTCCT



differentiation
(SEQ. ID. NO: 25)



factor 15


 




antisense
ACAGTTCCATCAGACCAGCC




(SEQ. ID. NO: 26)


 


BU587578
Regulator of G-protein
sense
CCGTGCCGGGTTTATCATTGCTTT



signalling 10
(SEQ. ID. NO: 27)


 




antisense
TCGACGTGTCCAGTGTTGAAGGAA




(SEQ. ID. NO: 28)


 


BQ683841
S100 calcium binding
sense
AGTCCCTGATTGCTGTCTTCCAGA



protein
(SEQ. ID. NO: 29)



All (calgizzarin)


 




antisense
ACCAGGGTCCTTCTGGTTCTTTGT




(SEQ. ID. NO: 30)


 


NM_0010136
polo-like kinase 3
sense
GACTACTCCAATAAGTTCGGCTTTG


32
(Drosophila)
(SEQ. ID. NO: 31)


 




antisense
CATATGTGTGCCATCGTTGAAGA




(SEQ. ID. NO: 32)


 


NM_032872
Synaptotagmin-like 1
sense
GGCGGTGAAGAAACGGAATCTGAA




(SEQ. ID. NO: 33)


 




antisense
GAAAGATGTTGCGACCCAGGCTTT




(SEQ. ID. NO: 34)


 


BC042390
Vesicle transport
sense
GGGGGACTAACCTATCGAGAA



through interaction
(SEQ. ID. NO: 35)



with t-SNAREs homolog



1B (yeast)


 




antisense
GATCCAGTGCTGATAGCAACC




(SEQ. ID. NO: 36)


 


NM_000596
Insulin-like growth
sense
ATCATTCCATCCTTTGGGACGCCA



factor binding protein 1
(SEQ. ID. NO: 37)


 




antisense
TGTCTCCTGTGCCTTGGCTAAACT




9SEQ. ID. NO: 38)


 


NM_005319
Histone 1, H1c
sense
ACCAAACCTAAGAAGCCAGTTG




(SEQ. ID. NO: 39)


 




antisense
TAGCGCTCTTCTTCGGAGTT




(SEQ. ID. NO: 40)


 


AK094474
Ras homolog gene
sense
ACCTGCCTCCTCATCGTCTTC



family, member C
(SEQ. ID. NO: 41)


 




antisense
CACCTGCTTGCCGTCCAC




(SEQ. ID. NO: 42)


 


NM_001769
CD9 antigen (p24)
sense
GCACCAAGTGCATCAAATACCTGC




(SEQ. ID. NO: 43)


 




antisense
AGCCATAGTCCAATGGCAAGGACA




(SEQ. ID. NO: 44)


 


NM_001553
Insulin-like growth
sense
ACTTGAGCTGTGAGGTCATCGGAA



factor binding protein 7
(SEQ. ID. NO: 45)


 




antisense
ATACCAGCACCCAGCCAGTTACTT




(SEQ. ID. NO: 46)


 


NM_003897
Immediate early
sense
AGCCCTTTAATCTGACTTCGGAGC



response 3
(SEQ. ID. NO: 47)


 




antisense
CAGGTACGCCTGGTGTTTCTTTGT




(SEQ. ID. NO: 48)


 


NM_003516
Histone 2, H2aa
sense
GAACTGAACAAGCTGCTGGGCAAA




(SEQ. ID. NO: 49)


 




antisense
TCTCCGTCTTCTTAGGGAGCAGTA




(SEQ ID. NO: 50)


 


AK074377
Chromosome 19 open
sense
CTGGTACGACGCGGAGAT



reading frame 31
(SEQ. ID. NO: 51)


 




antisense
CGACAGTCGTTCAGAGAATCA




(SEQ. ID. NO: 52)


 


BX640965
Solute carrier family
sense
GCCCTGTTCCAGCAATAAGA



22 (extraneuronal
(SEQ. ID. NO: 53)



monoamine



transporter), member 3


 




antisense
GAGAGCCAAAAATGTCCCAA




(SEQ. ID. NO: 54)


 


NM_018492
PDZ binding kinase
sense
TCTGGACTGAGAGTGGCTTTCACA




(SEQ. ID. NO: 55)


 




antisense
AGCCAAGCTTCTGCATAAACGGAG




(SEQ. ID. NO: 56)


 


CR600021
High-mobility group
sense
CTTGGCACGATATGCAGCAA



box 2
(SEQ. ID. NO: 57)


 




antisense
CAGCCAAAGATAAACAACCATATGA




(SEQ. ID. NO: 58)


 


NM_006101
Kinetochore
sense
TGCTACACATGTTGGGTCTGTA



associated 2
(SEQ. ID. NO: 59)


 




antisense
CCGAGAGATCTTCTGACATGC




(SEQ. ID. NO: 60)


 


NM_003318
TTK protein kinase
sense
TCATGCCCATTTGGAAGAGTCCCA




(SEQ. ID. NO: 61)


 




antisense
AGGCACAACCAAATCTCGGCATTC




(SEQ. ID. NO: 62)


 


U65410
MAD2 mitotic arrest
sense
ACTTAAATATCTCCCTACCTATA



deficient-like
(SEQ. ID. NO: 63)
CTGAGTCAA



1 (yeast)


 




antisense
TAGTAACTGTAGATGGAAAAACT




(SEQ. ID. NO: 64)
TGTGCTA


 


NM_001067
Topoisomerase (DNA)
sense
CTAGTTAATGCTGCGGACAACA



II alpha 170 kDa
(SEQ. ID. NO: 65)


 




antisense
CATTTCGACCACCTGTCACTT




(SEQ. ID. NO: 66)


 


AB091343
Centrosomal protein
sense
GCTGTGCATTTCTCTTGGCAGTGA



55 kDa
(SEQ. ID. NO: 67)


 




antisense
TAGACGCTGCTCACCACATTCTGT




(SEQ. ID. NO: 68)


 


BC022255
Spindle pole body
sense
GAATGGTTGAGATGTTTCTGGA



component 25
(SEQ. ID. NO: 69)



homolog (S. cerevisiae)


 




antisense
GCAATCAATTTTAACAAGTTATC




(SEQ. ID. NO: 70)
CTTT
















TABLE 5







Quantitative real time RT-PCR results














Microarray
Real time PCR


Accession

Gene
(Cy3/Cy5
(relative


number
Gene name
abbreviation
ratio)
ratio)










Up-regulated











NM_000499.3
Cytochrome P450, family1,
CYP1A1
88.34
863.07



subfamily A, polypeptide 1
(SEQ ID NO: 71)




BX649164
serpin peptidase
SERPINE1
29.05
38.54



inhibitor, clade E(nexin,
(SEQ ID NO: 72)





plasminogen activator






inhibitor type 1), member 1





NM_004881.2
Tumor protein p53
TP53I3
16.67
26.57



inducible protein 3
(SEQ ID NO: 73)




BM913048
TIMP metallopeptidase
TIMP1
14.52
26.48



inhibitor 1
(SEQ ID NO: 74)




NM_006426.2
Dihydropyrimidinase-like 4
DPYSL4
13.51
101.13




(SEQ ID NO: 75)




NM_133493.2
CD109 antigen(Gov
CD109
12.97
45.46



platelet alloantigens)
(SEQ ID NO: 76)




AB033073.2
Sulfatase 2
SULF2
12.78
13.78




(SEQ ID NO: 77)




NM_021603.3
FXYD domain containing ion
FXYD2
12.63
29.86



transport regulator 2
(SEQ ID NO: 78)




BC035124
MSFL2541
UNQ2541
11.28
13.61




(SEQ ID NO: 79)




Y11307
Cysteine-rich,
CYR61
8.79
12.85



angiogenic inducer, 61
(SEQ ID NO: 80)




AK095363
Aquaporin 3
AQP3
8.26
46.53




(SEQ ID NO: 81)




AK097009
phospholipid transfer
PLTP
6.84
46.74



protein
(SEQ ID NO: 82)




NM_004864.2
Growth differentiation
GDF15
6.65
38.76



factor 15
(SEQ ID NO: 83)




BU587578
Regulator of G-protein
RGS10
6.15
13.03



signalling 10
(SEQ ID NO: 84)




BQ683841
S100 calcium binding
S100A11
6.06
8.38



protein A11(calgizzarin)
(SEQ ID NO: 85)




NM_001013632.2
polo-like kinase 3
PLK3
5.34
9.23



(Drosophila)
(SEQ ID NO: 86)




NM_032872.1
Synaptotagmin-like 1
SYTL1
4.93
10.43




(SEQ ID NO: 87)




BC042390
Vesicle transport through
VTI1B
4.49
8.21



interaction with t-SNAREs
(SEQ ID NO: 88)





homolog 1B(yeast)





NM_000596.2
Insulin-like growth
IGFBP1
4.43
5.07



factor binding protein 1
(SEQ ID NO: 89)




NM_005319.3
Histone 1, H1c
HIST1H1C
4.26
3.38




(SEQ ID NO: 90)




AK094474
Ras homolog gene family,
RHOC
4.23
3.41



member C
(SEQ ID NO: 91)




NM_001769.2
CD9 antigen(p24)
CD9
4.15
1.71




(SEQ ID NO: 92)




NM_001553.1
Insulin-like growth
IGFBP7
3.47
11.02



factor binding protein 7
(SEQ ID NO: 93)




NM_003897.3
Immediate early response 3
IER3
3.32
6.84




(SEQ ID NO: 94)




NM_003516.2
Histone 2, H2aa
HIST2H2AA
3.05
3.47




(SEQ ID NO: 95)









Down-regulated











AK074377
Chromosome 19 open reading
UHRF1
0.16
0.19



frame 31
(SEQ ID NO: 96)




BX640965
Solute carrier family
SLC22A3
0.26
0.23



22(extraneuronal monoamine
(SEQ ID NO: 97)





transporter), member 3





NM_018492.2
PDZ binding kinase
PBK
0.28
0.21




(SEQ ID NO: 98)




CR600021
High-mobility group box 2
HMGB2
0.29
0.23




(SEQ ID NO: 99)




NM_006101.1
Kinetochore associated 2
KNTC2
0.30
0.26




(SEQ ID NO: 100)




NM_003318.3
TTK protein kinase
TTK
0.30
0.47




(SEQ ID NO: 101)




U65410
MAD2 mitotic arrest
MAD2L1
0.34
0.34



deficient-like 1(yeast)
(SEQ ID NO: 102)




NM_001067.2
Topoisomerase(DNA) II
TOP2A
0.45
0.14



alpha 170 kDa
(SEQ ID NO: 103)




AB091343
Centrosomal protein 55 kDa
C10orf3
0.45
0.63




(SEQ ID NO: 104)




BC022255
Spindle pole body
Spc25
0.54
0.25



component 25 homolog(S. cerevisiae)
(SEQ ID NO: 105)









As a result, the expression profiles of 25 up-regulated genes and 10 down-regulated genes were consistent with the result of oligo-microarray examining the gene expressions by the chrysene.

## Claims

1. A method for confirming the exposure to chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample of an experimental group, and from somatic cells of a control group, wherein the human somatic cells originate from human liver or liver cancer cells;
2) comparing the expression level of all selected genes of the sample with that of the control, wherein the selected genes are:
CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) (SEQ. ID. NO: 71), PAI [serpin peptidase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1] (SEQ. ID. NO: 72), Tumor protein p53 inducible protein 3 (SEQ. ID. NO: 73), TIMP metallopeptidase inhibitor 1 (SEQ. ID. NO: 74), Dihydropyrimidinase-like 4 (SEQ. ID. NO: 75), CD109 antigen (Gov platelet alloantigens) (SEQ. ID. NO: 76), Sulfatase 2 (SEQ. ID. NO: 77), FXYD domain containing ion transport regulator 2 (SEQ. ID. NO: 78), MSFL2541 (SEQ. ID. NO: 79), Cysteine-rich, angiogenic inducer, 61 (SEQ. ID. NO: 80), Aquaporin 3 (SEQ. ID. NO: 81), phospholipid transfer protein (SEQ. ID. NO: 82), Growth differentiation factor 15 (SEQ. ID. NO: 83), Regulator of G-protein signalling 10 (SEQ. ID. NO: 84), S100 calcium binding protein All (calgizzarin) (SEQ. ID. NO: 85), polo-like kinase 3 (Drosophila) (SEQ. ID. NO: 86), Synaptotagmin-like 1 (SEQ. ID. NO: 87), Vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (SEQ. ID. NO: 88), Insulin-like growth factor binding protein 1 (SEQ. ID. NO: 89), Histone 1, H1c (SEQ. ID. NO: 90), Ras homolog gene family, member C (SEQ. ID. NO: 91), CD9 antigen (p24) (SEQ. ID. NO: 92), Insulin-like growth factor binding protein 7 (SEQ. ID. NO: 93), Immediate early response 3 (SEQ. ID. NO: 94), H1stone 2, H2aa (SEQ. ID. NO: 95), Chromosome 19 open reading frame 31 (SEQ. ID. NO: 96), Solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SEQ. ID. NO: 97), PDZ binding kinase (SEQ. ID. NO: 98), High-mobility group box 2 (SEQ. ID. NO: 99), Kinetochore associated 2 (SEQ. ID. NO: 100), TTK protein kinase (SEQ. ID. NO: 101), MAD2 mitotic arrest deficient-like 1 (yeast) (SEQ. ID. NO: 102), Topoisomerase (DNA) II alpha 170 kDa (SEQ. ID. NO: 103), Centrosomal protein 55 kDa (SEQ. ID. NO: 104), and Spindle pole body component 25 homolog (S. cerevisiae) (SEQ. ID. NO: 105); and

3) determining that the sample is exposed to the chrysene, when the expression level of the genes of CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) (SEQ. ID. NO: 71), PAI [serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1] (SEQ. ID. NO: 72), Tumor protein p53 inducible protein 3 (SEQ. ID. NO: 73), TIMP metallopeptidase inhibitor 1 (SEQ. ID. NO: 74), Dihydropyrimidinase-like 4 (SEQ. ID. NO: 75), CD109 antigen (Gov platelet alloantigens) (SEQ. ID. NO: 76), Sulfatase 2 (SEQ. ID. NO: 77), FXYD domain containing ion transport regulator 2 (SEQ. ID. NO: 78), MSFL2541 (SEQ. ID. NO: 79), Cysteine-rich, angiogenic inducer, 61 (SEQ. ID. NO: 80), Aquaporin 3 (SEQ. ID. NO: 81), phospholipid transfer protein (SEQ. ID. NO: 82), Growth differentiation factor 15 (SEQ. ID. NO: 83), Regulator of G-protein signalling 10 (SEQ. ID. NO: 84), S100 calcium binding protein A11 (calgizzarin) (SEQ. ID. NO: 85), polo-like kinase 3 (Drosophila) (SEQ. ID. NO: 86), Synaptotagmin-like 1 (SEQ. ID. NO: 87), Vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (SEQ. ID. NO: 88), Insulin-like growth factor binding protein 1 (SEQ. ID. NO: 89), Histone 1, Hlc (SEQ. ID. NO: 90), Ras homolog gene family, member C (SEQ. ID. NO: 91), CD9 antigen (p24) (SEQ. ID. NO: 92), Insulin-like growth factor binding protein 7 (SEQ. ID. NO: 93), Immediate early response 3 (SEQ. ID. NO: 94), and H1stone 2, H2aa (SEQ. ID. NO: 95), is significantly up-regulated, compared with that of the control,
and when the expression level of the genes Chromosome 19 open reading frame 31 (SEQ. ID. NO: 96), Solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SEQ. ID. NO: 97), PDZ binding kinase (SEQ. ID. NO: 98), High-mobility group box 2 (SEQ. ID. NO: 99), Kinetochore associated 2 (SEQ. ID. NO: 100), TTK protein kinase (SEQ. ID. NO: 101), MAD2 mitotic arrest deficient-like 1 (yeast) (SEQ. ID. NO: 102), Topoisomerase (DNA) II alpha 170 kDa (SEQ. ID. NO: 103), Centrosomal protein 55 kDa (SEQ. ID. NO: 104), and Spindle pole body component 25 homolog (S. cerevisiae) (SEQ. ID. NO: 105) is significantly down-regulated, compared with that of the control.

2. The method according to claim 1, wherein the human liver cancer cells are HepG2.

3. The method according to claim 1, wherein the comparing of the expression of step 2) is performed as mRNA expression level.

4. The method according to claim 3, wherein the mRNA expression level comparison is performed by oligonucleotide or polynucleotide microarray, or RT-PCR.

5. A method for confirming the exposure to chrysene comprising the following steps:
1) separating RNAs from human somatic cells of a sample of an experimental group, and from somatic cells of a control group, wherein the human somatic cells originate from human liver or liver cancer cells;
2) synthesizing cDNAs with the RNAs separated from the experimental group and the control of step 1) and labeling them with different fluorescent materials;
3) hybridizing the cDNAs of step 2) each labeled with different fluorescent materials with DNA microarray chip comprising oligonucleotide containing the full length or complement oligonucleotides of all selected genes, wherein the selected genes are:
CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) (SEQ. ID. NO: 71), PAI [serpin peptidase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1] (SEQ. ID. NO: 72), Tumor protein p53 inducible protein 3 (SEQ. ID. NO: 73), TIMP metallopeptidase inhibitor 1 (SEQ. ID. NO: 74), Dihydropyrimidinase-like 4 (SEQ. ID. NO: 75), CD109 antigen (Gov platelet alloantigens) (SEQ. ID. NO: 76), Sulfatase 2 (SEQ. ID. NO: 77), FXYD domain containing ion transport regulator 2 (SEQ. ID. NO: 78), MSFL2541 (SEQ. ID. NO: 79), Cysteine-rich, angiogenic inducer, 61 (SEQ. ID. NO: 80), Aquaporin 3 (SEQ. ID. NO: 81), phospholipid transfer protein (SEQ. ID. NO: 82), Growth differentiation factor 15 (SEQ. ID. NO: 83), Regulator of G-protein signalling 10 (SEQ. ID. NO: 84), S100 calcium binding protein A11 (calgizzarin) (SEQ. ID. NO: 85), polo-like kinase 3 (Drosophila) (SEQ. ID. NO: 86), Synaptotagmin-like 1 (SEQ. ID. NO: 87), Vesicle transport through interaction with t-SNARES homolog 1B (yeast) (SEQ. ID. NO: 88), Insulin-like growth factor binding protein 1 (SEQ. ID. NO: 89), Histone 1, H1c (SEQ. ID. NO: 90), Ras homolog gene family, member C (SEQ. ID. NO: 91), CD9 antigen (p24) (SEQ. ID. NO: 92), Insulin-like growth factor binding protein 7 (SEQ. ID. NO: 93), Immediate early response 3 (SEQ. ID. NO: 94), H1stone 2, H2aa (SEQ. ID. NO: 95), Chromosome 19 open reading frame 31 (SEQ. ID. NO: 96), Solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SEQ. ID. NO: 97), PDZ binding kinase (SEQ. ID. NO: 98), High-mobility group box 2 (SEQ. ID. NO: 99), Kinetochore associated 2 (SEQ. ID. NO: 100), TTK protein kinase (SEQ. ID. NO: 101), MAD2 mitotic arrest deficient-like 1 (yeast) (SEQ. ID. NO: 102), Topoisomerase (DNA) II alpha 170 kDa (SEQ. ID. NO: 103), Centrosomal protein 55 kDa (SEQ. ID. NO: 104), and Spindle pole body component 25 homolog (S. cerevisiae) (SEQ. ID. NO: 105);
4) analyzing the DNA microarray chip reacted in step 3);
5) comparing the expression of all of the selected genes of the sample with that of the control, wherein the genes are regarded as being exposed to a chrysene; and
6) determining that the sample is exposed to the chrysene, when the expression level of the genes of CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) (SEQ. ID. NO: 71), PAI [serpin peptidase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1] (SEQ. ID. NO: 72), Tumor protein p53 inducible protein 3 (SEQ. ID. NO: 73), TIMP metallopeptidase inhibitor 1 (SEQ. ID. NO: 74), Dihydropyrimidinase-like 4 (SEQ. ID. NO: 75), CD109 antigen (Gov platelet alloantigens) (SEQ. ID. NO: 76), Sulfatase 2 (SEQ. ID. NO: 77), FXYD domain containing ion transport regulator 2 (SEQ. ID. NO: 78), MSFL2541 (SEQ. ID. NO: 79), Cysteine-rich, angiogenic inducer, 61 (SEQ. ID. NO: 80), Aquaporin 3 (SEQ. ID. NO: 81), phospholipid transfer protein (SEQ. ID. NO: 82), Growth differentiation factor 15 (SEQ. ID. NO: 83), Regulator of G-protein signalling 10 (SEQ. ID. NO: 84), S100 calcium binding protein A11 (calgizzarin) (SEQ. ID. NO: 85), polo-like kinase 3 (Drosophila) (SEQ. ID. NO: 86), Synaptotagmin-like 1 (SEQ. ID. NO: 87), Vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (SEQ. ID. NO: 88), Insulin-like growth factor binding protein 1 (SEQ. ID. NO: 89), Histone 1, H1c (SEQ. ID. NO: 90), Ras homolog gene family, member C (SEQ. ID. NO: 91), CD9 antigen (p24) (SEQ. ID. NO: 92), Insulin-like growth factor binding protein 7 (SEQ. ID. NO: 93), Immediate early response 3 (SEQ. ID. NO: 94), and H1stone 2, H2aa (SEQ. ID. NO: 95), is significantly up-regulated, compared with that of the control, and when the expression level of the genes Chromosome 19 open reading frame 31 (SEQ. ID. NO: 96), Solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SEQ. ID. NO: 97), PDZ binding kinase (SEQ. ID. NO: 98), High-mobility group box 2 (SEQ. ID. NO: 99), Kinetochore associated 2 (SEQ. ID. NO: 100), TTK protein kinase (SEQ. ID. NO: 101), MAD2 mitotic arrest deficient-like 1 (yeast) (SEQ. ID. NO: 102), Topoisomerase (DNA) II alpha 170 kDa (SEQ. ID. NO: 103), Centrosomal protein 55 kDa (SEQ. ID. NO: 104), and Spindle pole body component 25 homolog (S. cerevisiae) (SEQ. ID. NO: 105) is significantly down-regulated, compared with that of the control.

6. The method according to claim 5, wherein the human liver cancer cells are HepG2.

7. The method according to claim 5, wherein the fluorescent material of step 2) is selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate (RITC) and rhodamine.

8. The method according to claim 5, wherein the oligonucleotide or its complement oligonucleotide of step 3) contains 18-30 nucleotides of the gene whose expression is changed by chrysene.

9. The method according to claim 8, wherein the oligonucleotide or its complement oligonucleotide of step 3) contains 20-25 nucleotides of the gene whose expression is changed by chrysene.