p . 1

Table 2 Blank test of the test substance in fis h

W1 W2 A B C D peak sample fish peak area conc . conc .

weight count in final in fish No .l No .2 solutio n No . No . g g sample std . mg/L μg/g A 1 8 .82 7 .65 <912 18241 <0 .00025 <0 .16 2 7 .20 8 .51 <912 18241 <0 .00025 <0 .16 3 11 .74 15 .19 <1006 20116 <0 .00025 <0 .10 4 13 .74 13 .53 <1006 20116 <0 .00025 <0 .1 0 B 1 8 .82 7 .65 <922 18448 <0 .00025 <0 .16 2 7 .20 8 .51 <922 18448 <0 .00025 <0 .16 3 11 .74 15 .19 <1020 20402 <0 .00025 <0 .10 4 13 .74 13 .53 <1020 20402 <0 .00025 <0 .1 0 ~ C 1 8 .82 7 .65 <1089 21784 <0 .00025 <0 .16 2 7 .20 8 .51 <1089 21784 <0 .00025 <0 .16 3 11 .74 15 .19 <1201 24013 <0 .00025 <0 .10 4 13 .74 13 .53 <1201 24013 <0 .00025 <0 .1 0 D 1 8 .82 7 .65 <1235 24694 <0 .00025 <0 .16 2 7 .20 8 .51 <1235 24694 <0 .00025 <0 .16 3 11 .74 15 .19 <1376 27516 <0 .00025 <0 .10 4 13 .74 13 .53 <1376 27516 <0 .00025 <0 .10 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Equations : C=A=BXCstd ~ D=A=B xCstdx(FV/1000)x SF=(W 1+W2)x 1000

~o~ ~ -a&p . 2

Table 3 Recovery test of the test substance in fish A B C D E R peak sample fish test peak area conc . measured recovery

weight substance count in final amount No .l No .2 added solution Average Standard Range No . No . g g mg sample std . mg/L mg % n=4 deviation A 1 13 .98 4 .14 0 .05 15171 18232 0 .00416 0 .0416 83 .2 84 .6 3 .1 6 .9 2 12 .30 5 .73 0 .05 16018 18232 0 .00439 0 .0439 87. 9 3 12 .51 5 .98 0 .05 14766 18232 0 .00405 0 .0405 81 .0 4 12 .33 5 .91 0 .05 15718 18232 0 .00431 0 .0431 86 .2 B 1 13 .98 .4 .14 0 .05 15340 18549 0 .00413 0 .0413 82 .7 84 .6 3 .2 7 .0 2 12 .30 5 .73 0 .05 16335 18549 0 .00440 0 .0440 88 . 1 3 12 .51 5 .98 0 .05 15044 18549 0 .00406 0 .0406 81 .1 4 12 .33 5 .91 0 .05 16031 18549 0 .00432 0 .0432 86 . 4 C 1 13 .98 4 .14 0 .05 18824 21914 0 .00429 0 .0429 85 .9 86 .3 2 .3 5 .6 2 12 .30 5 .73 0 .05 19524 21914 0 .00445 0 .0445 89 . 1 3 12 .51 5 .98 0 .05 18304 21914 0 .00418 0 .0418 83 .5 4 12 .33 5 .91 0 .05 18969 21914 0 .00433 0 .0433 86. 6 D 1 13 .98 4 .14 0 .05 21029 24731 0 .00425 0 .0425 85 .0 86 .3 3 .9 9 .2 2 12 .30 5 .73 0 .05 22607 24731 0 .00457 0 .0457 91 . 4 3 12 .51 5 .98 0 .05 20333 24731 0 .00411 0 .0411 82 .2 4 12 .33 5 .91 0 .05 21373 24731 0 .00432 0 .0432 86 .4 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Equations : D=B=CxCstd

E= B=CXCstdx.(FV/1000)x SF t R=B=CxCstdx(FV/1000)xSF=Ax 100p . 3

Table 4-1 Concentration of the test substance in the test water - High concentration level (Pea k A) i A B C D sampling period number peak area conc . mean conc . date of count in water in water

_ day analysis sample std . mg/L mg/L 2006/05/11 0 1 18559 18464 0 .0503 0 .0503 2006/05/18 7 2 17072 18738 0 .0456 0 .0479 2006/05/25 14 3 74769 75391 0 .0496 0 .0485 2006/06/01 21 4 85849 79289 0 .0541 0 .0499 2006/06/08 28 5 41124 44333 0 .0464 0 .0492 2006/06/15 35 6 38036 36313 0 .0524 0 .0497 2006/06/22 42 7 25519 24612 0 .0518 0 .0500 2006/06/29 49 8 31049 34034 0 .0456 0 .0495 2006/07/10 60 9 29606 31130 0 .0476 0 .0493 Standard deviation : 0 .0032 mg/L Coefficient of variation : 6 .5 % Concentration in water at steady state (28-60day) : 0 .0488 mg/L Concentration of std . solution (Cstd) : 0 .005 mg/ L Dillution Ratio(DR) : 10 Equations : C=A=BxCstdxDR

D=E{A=BxCstdxDR}i= i Figure 7-1 Concentration of the test substance in the test water - High concentration level (Peak A)

0 .1 0 (~- analyzed value - - - - - - nominal value

.:, 0 .05 .- ------ ------ 

# I

_

0 .00 0 7 14 21 28 35 42 49 56 Exposure period (day )

~ 

## VI 3p . 4

T able 4-2 Concen tration of the test substance in the test water

- High concentration level (Pe ak B) _ i A B C D sampling period number peak area conc . mean conc . date of count in water in water

day analysis sample std . mg/L mg/L 2006/05/11 0 1 18560 18907 0 .0491 0 .0491 2006/05/18 7 2 17439 19010 0 .0459 0 .0475 2006/05/25 14 3 75647 76115 0 .0497 0 .0482 2006/06/01 21 4 86327 80849 0 .0534 0 .0495 2006/06/08 28 5 41472 44180 0 .0469 0 .0490 2006/06/15 35 6 38406 37115 0 .0517 0 .0495 2006/06/22 42 7 25544
-25176 0 .0507 0 .0496 2006/06/29 49 8 31847 34465 0 .0462 0 .0492 2066/07/10 60 9 30538 31254 0 .0489 0 .049 2 Standard deviation : 0 .0025 mg/L Coefficient of variation : 5 .1 % Concentration in water at steady state (28-60day) : 0 .0489 mg/L Concentration of std . solution ( Cstd) : 0 .005mg/L Dillution Ratio(DR) : 10 Equations : C=A=BxCstdxDR

D=E{A=BXCstdxDR} i= i Figure 7-2 Concen tration of the test substance in the test water
- High concentration level (Peak B) 0 .1 0

~ • analyzed value • • • • - . - nominal value O 0 .05 . . ., . ., . . . W O
0 .00 0 7 14 21 28 35 42 49 56 Exposure period (day)



# ~ ~~ ~p . 5

Table 4-3 Concentration of the test substance in the test water
- High concentration level (Peak C) i A B C D sampling period number peak area conc . me an conc . date of count in water in water

_ day analysis sample std . mg/L mg/L 2006/05/11 0 1 22666 22400 0 .0506 0 .0506 2006/05/18 7 2 21194 22125 0 .0479 0 .0492 2006/05/25 14 3 91144 91116 0 .0500 0 .0495 2006/06/01 21 4 104128 95473 0 .0545 0 .0508 2006/06/08 28 5 52259 53198 0 .0491 0 .0504 2006/06/15 35 6 46121 44829 0 .0514 0 .0506 2006/06/22 42 7 31410 30910 0 .0508 0 .0506 2006/06/29 49 8 38449 40090 0 .0480 0 .0503 2006/07/10 60 9 35701 36746 0 .0486 0 .050 1 ~ Standard deviation : 0 .0021 mg/L

Coefficient of variation : 4 .2 % Concentration in water at steady state (28-60day) : 0 .0496 mg/L Concentration of std. solution (Cstd) : 0 .005mg/L Dillution Ratio(DR) : 10 Equations : C=A=BxCstdxDR

D=E{A=BxCstdxDR}i=i Figure 7-3 Concen tration of the test substance in the test water - High concentration level (Peak C) 0 .1 0 ( --~- analyzed value

~ nominal valu e ~ I o ~ 0 .05 ~. ~ 0 U

0 .00 0 7 14 21 28 35 42 49 56 Exposure period (day)

~f'r lo~ S 'p . 6

Table 4-4 Concentration of the test substance in the test water - High concentration level (Pe ak D) i A B C D sampling period number peak area conc . mean conc . date of count in water in water _ day analysis sample std . mg/L mg/L 2006/05/11 0 1 25629 24805 0 .0517 0 .0517 2006/05/18 7 2 23854 24927 0 .0478 0 .0498 2006/05/25 14 3 103625 102473 0 .0506 0 .0500 2006/06/01 21 4 120550 109283 0 .0552 0 .0513 2006/06/08 28 5 58104 60332 0 .0482 0 .0507 2006/06/15 35 6 52233 50190 0 .0520 0 .0509 2006/06/22 42 7 34926 33664 0 .0519 0 .0510 2006/06/29 49 8 42529 46765 0 .0455 0 .0503 2006/07/10 60 9 39797 42469 0 .0469 0 .050 0 { Standard deviation : 0 .0031 mg/L

Coefficient of variation : 6 .2 % Concentration in water at steady state (28-60day) : 0 .0489 mg/L Concentration of std . solution (Cstd) : 0 .005mg/ L Dillution Ratio(DR) : 10 Equations : C=A=BxCstdxDR

D =E {A=B XCstdXDR} i=i Figure 7-4 Concentration of the test substance in the test water - High concentration level (Peak D) 0 .1 0

~ • analyzed valu e

r . '

. . . . . nominal value on
O
. . . . . . . . . . . . . . . . . . . ., . . . . . . . . . , . ., . ., . . . . . t4 0 .0 5
U r_ O U
0 .00 0 7 14 21 28 35 42 49 56 Exposure period (day)

-~- kCp . 7

Table 5-1 Concentration of the test substance in the test water - Low concentration level (Peak A ) i A B C D sampling period number peak area conc . mean conc. date of count in water in wate r

day analysis sample std mglL mg/L 2006/05/11 0 1 18713 18464 0 .00507 0 .00507 2006/05/18 7 2 17009 18738 0 .00454 0 .00480 2006/05/25 14 3 75021 75391 0 .00498 0 .00486 2006/06/01 21 4 77682 79289 0 .00490 0 .00487 2006/06/08 28 5 43617 44333 0 .00492 0 .00488 2006/06/15 35 6 37321 36313 0 .00514 0 .00492 2006/06/22 42 7 24741 24612 0 .00503 0 .00494 2006/06/29 49 8 33553 34034 0 .00493 0 .00494 2006/07/10 60 9 32890 31130 0 .00528 0 .00498 Standard deviation : 0 .00020 mg/I , Coefficient of variation : 4 .0 % Concentration in water at steady state (28-60day) : 0 .00506 mg/L Concentration of std . solution ( Cstd) : 0 .005rng/L Equations : C=A=BxCstd D=E{ABxCstd}i=i Figure 8-1 Concentration of the test subst ance in the test water

- Low concentration level (Peak A) 0 .010

• analyzed value

. • . . •• . nominal value au E~ 0 0 .005 c I U U

0 .000 0 7 14 21 28 35 42 49 56 Exposure period (day)

-R,8- ~~ ~p . 8

Table 5-2 Concentration of the test substance in the test water - Low concentration level (Peak B )

_ i A B C D sampling period number peak area conc . mean conc . date of count in water in wate r

day analysis sample std . mg/L mg/L 2006/05/11 0 1 19002 18907 0 .00503 0 .00503 2006/05/18 7 2 17294 19010 0 .00455 0 .00479 2006/05/25 14 3 75794 76115 0 .00498 0 .00485 2006/06/01 21 4 79041 80849 0 :00489 0 .00486 2006/06/08 28 5 44161 44180 0 .00500 0 .00489 2006/06/15 35 6 37752 37115 0 .00509 0 .00492 2006/06/22 42 7 24770 25176 0 .00492 0 .00492 2006/06/29 49 8 33658 34465 0 .00488 0 .00492 2006/07/10 60 9 33223 31254 0 .00531 0 .00496 ~ Standard deviation : 0 .00020 mg/L

Coefficient of variation : 4 .0 % Concentration in water at steady state (28-60day) : 0 .00504 mg/L Concentration of std . solution (Cstd) : 0 .005mg/L Equations : C=A=BxCstd

D=E{A=BxCstd }i=i Figure 8-2 Concentration of the test substance in the test water - Low concentration level (Peak B) 0 .010

- ♦ - analyzed value

. . . . . . . nominal value
.'" ' . 0 0.005 . . . . . . cd i . ~ r N U O U
0 .000 0 7 14 21 28 35 42 49 56 Exposure period (day)p . 9

Table 5-3 Concentration of the test substance in the test water - Low concentration level (Peak C ) i A B C D sampling period number peak area conc . mean conc . date of count in water in wate r

day analysis sample std . mg/L mg/L 2006/05/11 0 1 22689 22400 0 .00506 0 .00506 2006/05/18 7 2 21014 22125 0 .00475 0 .00491 2006/05/25 14 3 91219 91116 0 .00501 0 .00494 2006/06/01 21 4 95653 95473 0 .00501 0 .00496 2006/06/08 28 5 54099 53198 0 .00508 0 .00498 2006/06/15 35 6 45702 44829 . 0 .00510 0 .00500 2006/06/22 42 7 31673 30910 0 .00512 0 .00502 2006/06/29 49 8 40844 40090 0 .00509 0 .00503 2006/07/10 60 9 39756 36746 0 .00541 0 .00507 ~ Standard deviation : 0 .00017 mg/L

Coefficient of variation : 3 .4 % Concentration in water at steady state (28-60day) : 0 .00516 mg/L Concentration of std . solution (Cstd) : 0 .005 mg/L Equations : C=A=BxCstd

D=E{A=BxCstd }i=i Figure 8-3 Concentration of the test substance in the test water - Low concentration level (Peak C) 0 .010 ~- analyzed value t -a 

### . . . . . . . nominal value

6b

. . : . ~ ~ 0 .00 5 ~ ~ a a~ U C O
0 .000 0 7 14 21 28 35 42 49 .56 Exposure period (day )


## ,4-0- l~-l `~P . 10

Table 5-4 Concentration of the test substance in the test water - Low concentration level (Peak D) i A B C D sampling period number peak area conc . mean conc . date of count in water in wate r

day analysis sample std . mg/L mg/L 2006/05/11 0 1 25476 24805 0 .00514 0 .00514 2006/05/18 7 2 23766 24927 0 .00477 0 .00495 2006/05/25 14 3 103684 102473 0 .00506 0 .00499 2006/06/01 21 4 108755 109283 0 .00498 0 .00498 2006/06/08 28 5 60647 60332 0 .00503 0 .00499 2006/06/15 35 6 51994 50190 0 .00518 0 .00502 2006/06/22 42 7 34296 33664 0 .00509 0 .00503 2006/06/29 49 8 45521 46765 0 .00487 0 .00501 2006/07/10 60 9 44931 42469 0 .00529 0 .00504 ~ Standard deviation : 0 .00016 rng/L

Coefficient of variation : 3 .2 % Concentration in water at steady state (28-60day) : 0 .00509 mg/L Concentration of std. solution (Cstd) : 0 .005 mg/ L Equations : C=A=BXCstd

D=E{A=BxCstd }i= i Figure 8-4 Concentration of the test substance in the test water - Low concentration level (Peak D) 0 .010 -+- analyzed value

~ -~ .•••••• nominal value

~ c - 0 0 .005
~. ~ ~ ~ U

~ O U
0.000 0 7 14 21 28 35 42 49 56 Exposure period (day)



## ..4}- l,o~ c~P . 11

Table 6-1 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - High concentration level (Peak A) W1 W2 A B C DR D E F G exposure fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .l No .2 count solution body in water day No . g g sample std . mg/L μg/g mg/L 7 1 10 .98 9 .17 39672 39020 0 .00508 20 59 .6 0 .0479 1250 1310 7 2 11 .57 9 .47 45238 39020 0 .00580 20 65 .1 0 .0479 136 0 14 1 13 .78 7 .07 54054 64364 0 .00420 20 47 .6 0 .0485 982 1110 14 2 9 .93 11 .15 68514 64364 0 .00532 20 59 .7 0 .0485 123 0 28 1 15 .93 9 .38 56125 43338 0 .00648 20 60 .5 0 .0492 1230 1120 28 2 10 .09 9 .25 34925 43338 0 .00403 20 49 .3 0 .0492 100 0 35 1 6 .22 18 .80 40239 36580 0 .00550 20 52 .0 0 .0497 1050 1090 35 2 11 .61 14 .68 45751 36580 0 .00625 20 56 .2 0 .0497 113 0 60 1 10 .47 12 .76 52589 32770 0 .00802 20 81 .7 0 .0493 1660 1380

~ 60 2 8 .59 15 .26 35857 32770 0 .00547 20 54 .2 0 .0493 1100

Concentration in fish body at steady state (28-60day) : 59 .0 μg/g Concentration in water at steady state (28-60day) : 0 .0488 mg/L BCF at steady state (BCFss) : 1210 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd

D=A=B XCstdXDRx (FV/1000)x SF=(W 1+W2)=(R/100)x 1000 F=A=Bx CstdxDRx(FV/1000)x SF-(W 1+W2)-(R/100)X 1000=E G=(F1+F2)-2 Figure 9-1 Bioconcentration factor (BCF) - High concentration level (PeakA) { 10000 ----------

------------
------------ 

### -------------------------------------

--------------------------------------------------------- -- ------------------------------------------------------ U 1000 -- ---------- -----------

--------------------------------=----------------------

---------------------------------------------------------

--------------------------------------------------------- 100 0 7 14 21 28 35 42 49 56 Exposure period (day)



### -42- k, :,gp .12

Table 6-2 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - High concentration level (Peak B ) W1 W2 A B C DR D E F G exposur fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .1 No .2 count solution body in water day No . g sample std . mg/L μg/g mg/L 7 1 10 .98 9 .17 41601 39366 0 .00528 20 62 .0 0 .0475 1310 1370 7 2 11 .57 9 .47 47458 39366 0 .00603 20 67 .7 0 .0475 143 0 14 1 13 .78 7 .07 57332 65291 0 .00439 20 49 .8 0 .0482 1030 1160 14 2 9 .93 11 .15 72040 65291 0 .00552 20 61 .9 0 .0482 128 0 28 1 15 .93 9 .38 59412 43935 0 .00676 20 63 .2 0 .0490 1290 1170 28 2 10 .09 9 .25 36957 43935 0 .00421 20 51 .6 0 .0490 105 0 35 1 6 .22 18 .80 42744 36858 • 0 .00580 20 54 .8 0 .0495 1110 1160 35 2 11 .61 14 .68 48853 36858 0 .00663 20 59 .6 0 .0495 120 0 60 1 10 .47 12 .76 55704 . 32763 0 .00850 20 86 .5 0 .0492 1760 1460 60 2 8 .59 15 .26 37789 32763 0 .00577 20 57 .2 0 .0492 1160 Concentration in fish body at steady state (28-60day) : 62 .1 pg/g Concentration in water at steady state (28-60day) : 0 .0489 mg/L BCF at steady state (BCFss) : 1270 Concentration of std . solution (Cstd) : 0 .005 rng/L Final volume (FV) : 10ML Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd

D=A=BxCstdxDRx(FV/1000)x SF-* (W 1+W2)=(R/100)x 1000 F=A=B XCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000=E G=(F1+F2)-2 Figure 9-2 Bioconcentration factor (BCF) - High concentration level (PeakB)

10000 

# F ---------



### ----------------- --------------------------------------

---------------------------- -----------------

-------

---------------------------------------------- U 1000 ------------------------

---- 

# -------------------- 100 0 7 14 21 28 35 42 49 56 Exposure period (day)p . 13

Table 6-3 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - High concen tration level (Peak C ) W1 W2 A B C DR D E F G exposure fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .1 No .2 count solution body in water day No . g g sample std . mg/L 119/9 mg/L 7 1 10 .98 9 .17 37232 47074 0 .00395 20 45 .5 0 .0492 924 972 7 2 11 .57 9 .47 42823 47074 0 .00455 20 50 .1 0 .0492 102 0 14 1 13 .78 7 .07 50898 79428 0 .00320 20 35 .6 0 .0495 719 807 14 2 9 .93 11 .15 63995 79428 0 .00403 20 44 .3 0 .0495 89 5 28 1 15 .93 9 .38 51919 53732 0 .00483 20 44 .2 0 .0504 878 790 28 2 10 .09 9 .25 31730 53732 0 .00295 20 35 .4 0 .0504 702 35 1 6 .22 18 .80 37325 44963 0 .00415 20 38 .4 0 .0506 760 795 35 2 11 .61 14 .68 42819 44963 0 .00476 20 42 .0 0 .0506 83 0 60 1 10 .47 12 .76 48410 38267 0 .00633 20 63 .1 0 .0501 1260 1050 60 2 8 .59 15 .26 33297 38267 0 .00435 20 42 .3 0 .0501 844 Concentration in fish body at steady state (28-60day) : 44 .2 μg/g Concentration in water at steady state (28-60day) : 0 .0496 mg/L BCF at steady state (BCFss) : 891 Concentration of std . solution (Cstd) : O .OOSmg/I, Final volume (FV) : lOmL Sampling factor (SF) : 100 0 Recovery (R) : 86 .3% Equations : C=A=BxCst d

D=A- . BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000 F=A=BxCstdxDRx(FV/1000)XSF=(W 1 +W2)=(R/100)x 1000=E G=(F1+F2)-2

~ Figure 9-3 Bioconcentration factor (BCF) - High concentration level (PeakC) 10000

--------------------------------------------------------

--------------------------------------------------------

---------------------------------------------------------

-------------------------------------- 1000 -- --- #-- _______---- ~ _____~=======================

------------------------------

----------------------------------------------------------

---------------------------------------------------------- 100 1 0 7 14 21 28 35 42 49 56 Exposure period (day)

lo$ 3 -44-p . 14

Table 6-4 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - High concentration level (Peak D) W1 W2 A B C DR D E F G exposure fish peak area cone . Dilution cone . mean BCF mean period weight in final ratio in fish cone . BCF

No .1 No .2 count solution body in wate r day No . g g sample std . mg/L μg/g mg/L 7 1 10 .98 9 .17 39862 52665 0 .00378 20 43 .5 0 .0498 874 922 7 2 11 .57 9 .47 46175 52665 0 .00438 20 48 .3 0 .0498 970 14 1 13 .78 7 .07 53718 89576 0 .00300 20 33 .3 0 .0500 667 744 14 2 9 .93 11 .15 66801 89576 0 .00373 20 41 .0 0 .0500 82 0 28 1 15 .93 9 .38 55755 59749 0 .00467 20 42 .7 0 .0507 843 758 28 2 10 .09 9 .25 34047 59749 0 .00285 20 34 .1 0 .0507 67 3 35 1 6 .22 18 .80 39928 49711 0 .00402 20 37 .2 0 .0509 731 763 35 2 11 .61 14 .68 45662 49711 0 .00459 20 40 .5 0 .0509 79 5 f 60 1 10 .47 12 .76 51033 43233 0 .00590 20 58 .9 0 .0500 1180 988

60 2 8 .59 15 .26 35429 43233 0 .00410 20 39 .8 0 .0500 796 Concentration in fish body at steady state (28-60day) : 42 .2 μg/g Concentration in water at steady state (28-60day) : 0 .0489 mg/L BCF at steady state (BCFss) : 863 Concentration of std, solution (Cstd) : 0 .0051ng/L Final volume (FV) : I OmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=A+BxCstd

D =A=BXCstdXDRx FV/1000 XSF= Wl+W2 = R/100 x100

( ) ( )( ) 0 F=A=B XCstdXDRx(FV/1000)x SF-(W 1+W2)=(R/100)X 1000=E G=(F1+F2)-2

~ Figure 9-4 Bioconcentration factor (BCF) - High concentration level (PeakD) 10000 ------ --------
------------------------ -------- ---------------------------------------------------
--------
----------------------------------------------------- --=--------------------------------------------------

----------------------------------------------------

---------------------------------------------------- w 1000 ____-__-___~=========== --===~=====================

----------------------------------------------------

------------------------------------------------------- 100 ' 0 7 14 21 28 35 42 49 56 Exposure period (day)p . 15

Table 7-1 Concentration of the test substance in the test fish and bioconcentration factor (BCF)



### - Low concentration level (Peak A ) W1 W2 A B C DR D E F G exposure fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .l No .2 count solution body in water day No . g g sample std . mg/L μg/g mg/L 7 1 14 .70 7 .81 49089 39020 0 .00629 2 6 .61 0 .00480 1380 1290 7 2 10 .82 9 .36 38172 39020 0 .00489 2 5 .73 0 .00480 119 0 14 1 7 .97 10 .30 125720 64364 0 .00977 1 6 .32 0 .00486 1300 1450 14 2 9 .47 9 .82 81146 64364 0 .00630 2 7 .73 0 .00486 159 0 28 1 14 .30 7 .65 47279 43338 0 .00545 2 5 .87 0 .00488 1200 1200 28 2 10 .44 12 .83 49524 43338 0 .00571 2 5 .80 0 .00488 119 0 35 1 6 .50 17 .00 43534 36580 0 .00595 2 5 .99 0 .00492 1220 1240 35 2 13 .50 8 .17 41325 36580 0 .00565 2 6 .16 0 .00492 125 0 60 1 11 .91 12 .66 51321 32770 0 .00783 2 7 .53 0 .00498 1510 1520 60 2 8 .48 17 .51 55046 32770 0 .00840 2 7 .64 0 .00498 1530 Concentration in fish body at steady state (28-60day) : 6 .50 μg/g Concentration in water at steady state (28-60day) : 0 .00506 mg/L BCF at steady state (BCFss) : 1280 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd

D =A=BxCstdxDRx(FV/1000)XSF=(W 1+W2)=(R/100)x 1000 F=A=BxCstdxDRX(FV/1000)xSF=(W1+W2)=(R/100)x 1000=E G = (F I +F2)-2 Figure 10-1 Bioconcentration factor (BCF) - Low concentration level (Peak A) 10000

--------------------

------------------------------------------------------------
------------------------------------------------------------ ----------------------------------=-------------------------

----------------------------------------------------------

----------------------------------------------------------

--------------------------------------------------------------

------------------------------------------------ ---------- ~ 1000

------------------

--------------------------------------------------------

----------------------------------------------------------

----------------------------------------------------------

-------------------------------------------------------------- 100 0 7 14 21 28 35 42 49 56
. Exposure period (day)p . 16

Table 7-2 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - Low concentration level (Peak B ) W1 W2 A B C DR D E F G exposure fish peak area conc . Dilution conc. mean BCF mean period weight in fi nal ratio in fish conc . BCF

No .l No.2 count solution body in wate r day No . g g sample std . mg/L μg/g mg/L 7 1 14 .70 7 .81 51481 39366 0 .00654 2 6 .87 0 .00479 1430 1350 7 2 10 .82 9 .36 40528 39366 0 .00515 2 . 6 .03 0 .00479 126 0 14 1 7 .97 10 .30 132535 65291 0 .01015 1 6 .57 0 .00485 1350 1510 14 2 9 .47 9 .82 86205 65291 0 .00660 2 8 .09 0 .00485 167 0 28 1 14 .30 7 .65 49887 43935 0 .00568 2 6 .11 0 .00489 1250 1250 28 2 10 .44 12 .83 52763 43935 0 .00600 2 6 .10 0 .00489 125 0 35 1 6 .50 17 .00 45929 36858 0 .00623 2 6 .27 0 .00492 1270 1290 35 2 13 .50 8 .17 43405 36858 0 .00589 2 6 .42 0 .00492 131 0 ( 60 1 11 .91 12 .66 54512 32763 0 .00832 2 8 .00 0 .00496 1610 1630

60 2 8 .48 17 .51 58750 32763 0 .00897 2 8 .16 0 .00496 1640 Concentration in fish body at steady state (28-60day) : 6 .84 μg/g Concentration in water at steady state (28-60day) : 0 .00504rng/L BCF at steady state (BCFss) : 1360 Concentration of std . solution (Cstd) : 0 .005 mg/L Final volume (FV) : 10mL Sampling factor ( SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BXCstd

D=A=BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000 F=A=BXCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000=E G=(F1+F2)-2 Figure 10-2 Bioconcentration factor (BCF) - Low concentration level (Peak B) 10000 ---- ---- 

### --------- - ------------------------ - ---------- ----------------------------------------------------------- ---------------------------------- -----------

-----------------------------------------------

----------------------------------------------------------

-------------------------------------------------------------

--------------------------------------------- 

### -------------- U 

### 1000

---------------------------------------------------------

-------------------------------------- -----------

---------------------------------------------------

-------------------------------------------------------------

------------------------------------------------------------- 100 ' 

### 0 7 14 21 28 35 42 49 56 Exposure period (day)
~-' Co$p . 17

Table 7-3 Concentration of the test substance in the test fish and bioconcentration factor (BCF )



### - Low concentration level (Peak C ) WI W2 A B C DR D E F G exposure fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .l No .2 count solution body in water day No . g g sample std . mg/L μg/ mg/L 7 1 14 .70 7 .81 46491 47074 0 .00494 2 5 .08 0 .00491 1040 971 7 2 10 .82 9 .36 36284 47074 0 .00385 2 4 .43 0 .00491 90 1 14 1 7 .97 10 .30 113720 79428 0 .00716 1 4 .54 0 .00494 919 1030 14 2 9 .47 . 9 .82 74927 79428 0 .00472 2 5 .67 0 .00494 115 0 28 1 14 .30 7 .65 43885 53732 0 .00408 2 4 .31 0 .00498 866 858 28 2 10 .44 12 .83 45620 53732 0 .00425 2 4 .23 0 .00498 84 9 35 1 6 .50 17 .00 39303 44963 0 .00437 2 4 .31 0 .00500 862 874 35 2 13 .50 8 .17 37211 44963 0 .00414 2 4 .43 0 .00500 88 5 60 1 11 .91 12 .66 46278 38267 0 .00605 2 5 .70 0 .00507 1120 1140 60 2 8 .48 17 .51 50649 38267 0 .00662 2 5 .90 0 .00507 1160 Concentration in fish body at steady state (28-60day) : 4 .81 μ.g/g Concentration in water at steady state (28-60day) : 0 .00516 mg/L BCF at steady state (BCFss) : 932 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : 10mL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=A=BXCstd

D=A=B xCstdxDRx(FV/1000)X SF=(W 1+W2)=(R/100)x 1000 F=A=B xCstdXDRx(FV/1000)x SF~(W 1+W2)=(R/ 100)x 1000=E G=(F1+F2)-2 ( Figure 10-3 Bioconcentration factor (BCF) - Low concentration level (Peak C)

10000

-----------------------------------------------------------

------------------------------------------- U 1000

_

------------------------------------------------------------ ------------------------------------------------------------ ------------------------------------------------------------

-------------------- -------------------------------------- 100 0 7 14 21 28 35 42 49 56 Exposure period (day)p . 18

Table 7-4 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- Low concentration level (Peak D) W1 W2 A B C DR D E F G exposure fish peak area conc . Dilution conc . mean BCF mean period weight in final ratio in fish conc . BCF

No .1 No .2 count solution body in water day No . g g sample std . mg/L [Ig/g mg/L 7 1 14 .70 7 .81 49968 52665 0 .00474 2 4 .88 0 .00495 987 924 7 2 10 .82 9 .36 39044 52665 0 .00371 2 4 .26 0 .00495 86 0 14 1 7 .97 10 .30 121296 89576 0 .00677 1 4 .29 0 .00499 861 966 14 2 9 .47 9 .82 79668 89576 0 .00445 2 5 .34 0 .00499 107 0 28 1 14 .30 7 .65 46330 59749 0 .00388 2 4 .09 0 .00499 820 816 28 2 10 .44 12 .83 48573 59749 0 .00406 2 4 .05 0 .00499 81 1 35 1 6 .50 17 .00 41724 49711 0 .00420 2 4 .14 0 .00502 824 837 35 2 13 .50 8 .17 39640 49711 0 .00399 2 4 .26 0 .00502 849 60 1 11 .91 12 .66 49241 43233 0 .00569 2 5 .37 0 .00504 1070 1090 60 2 8 .48 17 .51 53518 43233 0 .00619 2 5 .52 0 .00504 1100 Concentration in fish body at steady state (28-60day) : 4 .57 μg/g Concentration in water at steady state (28-60day) : 0 .00509 mg/L BCF at steady state (BCFss) : 898 Concentration of std . solution (Cstd) : 0 .005 mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3 % Equations

: C=A=BXCstd D=A=B xCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000 F=A=BxCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000=E G =(F 1+F2)-2 Figure 10-4 Bioconcentration factor (BCF) - Low concentration level (Peak D)

1000 0
w ----------------------------------------------------- U 1000

-----------------------------------------------------

----------------------------------------------------- 100 I 0 7 14 21 28 35 42 49 56 Exposure period (day )


### -49- ~ocbgP . 19

Table 8 Concentration of the test substance in the tissues and bioconcentration factor (BCF)

- High concentration level Peak A W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L μg/g mg/ L head 11 .39 29352 19311 0 .00760 10 78 .9 0 .0493 1600 viscera 5 .52 24842 19311 0 .00643 10 137 .7 0 .0493 2790 muscle & bones 16 .04 17470 19311 0 .00452 10 33 .3 0 .0493 676 fins & skin (with scales) 4 .91 27284 19311 0 .00706 5 85 .0 0 .0493 1720 Concentration of std . solution (Cstd) : 0 .005 mg/L Final volume (FV) : 10mL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Peak B W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L μg/g mg/L head 11 .39 31321 19508 0 .00803 10 83 .3 0 .0492 1690 viscera 5 .52 26712 19508 0 .00685 10 146 .6 0 .0492 2980 muscle & bones 16 .04 18705 19508 0 .00479 10 35 .3 0 .0492 718 fins & skin (with scales) 4 .91 28941 19508 0 .00742 5 89 .3 0 .0492 1810 Concentration of std. solution (Cstd) : 0 .005rng/L Final volume (FV) : 10 mL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCst d D=A=BXCstdxDRx(FV/1000)xSF=W=(R/100)x 100 0 

### F = A=B x CstdXDRx(FV/1000)xSF=W=(R/100)x 1000=E

~ ~n$°lp . 20

Table 8 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- High concentration level (continued) Peak C W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L F,g/g mg/L head 11 .39 26838 23284 0 .00576 10 58 .6 0 .0501 1170 viscera 5 .52 22585 23284 0 .00485 10 101 .8 0 .0501 2030 muscle & bones 16 .04 16023 23284 0 .00344 10 24 .9 0 .0501 496 fins & skin (with scales) 4 .91 24140 23284 0 .00518 5 61 .2 0 .0501 1220 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Peak D W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L μg/g mg/L head 11 .39 28359 26217 0 .00541 10 55 .0 0 .0500 1100 viscera 5 .52 23912 26217 0 .00456 10 95 .7 0 .0500 1910 muscle & bones 16 .04 16944 26217 0 .00323 10 23 .3 0 .0500 467 fins & skin (with scales) 4 .91 25758 26217 0 .00491 5 58 .0 0 .0500 1160 ~ Concentration of std . solution (Cstd) : 0 .005mg/ L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=ABXCstd D=A=B xCstdxDRx(FV/1000)xSF=W=(R/100)x 1000 F=A=BxCstdxDRx(FV/1000)XSF=W=(R/100)x 1000= E



# ~- ~,~ dp . 21

Table 9 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- Low concentration level Peak A W A B C DR D E F tissue tissue peak area conc . dilution cone . mean BCF weight in final ratio in fish cone .

count solution body in water g sample 'std . mg/L' gg/g mg/L head 10 .76 33287 17953 0 .00927 1 10 .18 0 .00498 2050 viscera 4 .38 25620 17953 0 .00714 1 19 .26 0 .00498 3870 muscle & bones 18 .88 22462 17953 0 .00626 1 3 .92 0 .00498 786 fins & skin (with scales) 4 .49 10642 17953 0 .00296 1 7 .80 0 .00498 1570 Concentration of std. solution (Cstd) : 0 .005 mg/ L Final volume (FV) : 10mL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Peak B W A B C DR D E F tissue tissue peak area cone . dilution cone . mean BCF weight in final ratio in fish conc ,

count solution body in water g sample std . mg/L μg/g mg/L head 10 .76 35457 17947 0 .00988 1 10 .85 0 .00496 2190 viscera 4 .38 27397 17947 0 .00763 1 20 .60 0 .00496 4150 muscle & bones 18 .88 23755 17947 0 .00662 1 4 .14 0 .00496 835 fins & skin (with scales) 4 .49 11310 17947 0 .00315 1 8 .30 0 .00496 1670 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : l OmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd D= A=B x Cstd x DRx (FV/ 1000) x SF=W=(R/ 100) x 1000 FA=BXCstdxDRx(FV/1000)x SF=W=(R/100)X 1000=Ep . 22

Table 9 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- Low concentration level (continued) Peak C W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L μg/g mg/L head 10 .76 29935 21712 0 .00689 1 7 .42 0 .00507 1460 viscera 4 .38 22485 21712 0 .00518 1 13 .70 0 .00507 2700 muscle & bones 18 .88 20050 21712 0 .00462 1 2 .83 0 .00507 559 fins & skin (with scales) 4 .49 9245 21712 0 .00213 1 5 .49 0 .00507 1080 Concentration of std . solution (Cstd) : 0 .005rng/L, Final volume (FV) : l OmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Peak D W A B C DR D E F tissue tissue peak area conc . dilution conc . mean BCF weight in final ratio in fish conc .

count solution body in water g sample std . mg/L μg/g mg/L head 10 .76 31992 24272 0 .00659 1 7 .10 0 .00504 1410 viscera 4 .38 24243 24272 0 .00499 1 13 .21 0 .00504 2620 muscle & bones 18 .88 21285 24272 0 .00438 1 2 .69 0 .00504 534 fins & skin (with scales) 4 .49 10083 24272 0 .00208 1 5 .36 0 .00504 1060 { Concentration of std . solution (Cstd) : 0 .005rng/L

Final volume (FV) : I OmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=A=BxCst d D=A=B xCstdxDRX(FV/ 1000) x SF=W=(R/100)x 1000 F=A=B XCstd XDRx(FV/1000) x SF=W=(R/ (R/1 x 1000=Ep . 23

Table 10-1 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak A) W, WZ A B C DR D E depuration fish peak area cone . Dilution conc . residual period weight in final ratio in fish rate

No .l No .2 count solution bod y day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 59 .0 100 .0 1 1 16 .65 9 .07 57757 32770 0 .00881 10 40 .5 71 .9 1 2 10 .19 11 .40 53146 32770 0 .00811 10 44 .4 2 1 8 .06 16 .32 40792 31335 0 .00651 10 31 .6 59 .0 2 2 11 .38 11 .02 45132 31335 0 .00720 10 38 . 0 4 1 14 .47 8 .89 21525 21570 0 .00499 10 25 .2 30 .8 4 2 10 .67 10 .08 16986 21570 0 .00394 5 11 .2 8 1 12 .99 6 .63 45761 25485 0 .00898 1 5 .4 9 .1 8 2 11 .62 9 .98 24670 25485 0 .00484 2 5 .3 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd

D =A=B xCstdXDRx(FV/1000)XSF=(W 1+W2)=(R/100)x 1000 E = (Di 1+Di2)-2=Do x 10 0 Figure 11-1 Concentration of the test substance in the test fish during depuration test

- High concentration level (PeakA)



### ~ 

## 100 ------- - - -- - -- - --- - - -- --- - ----- - ------- ---- - ----------- -------------------------------------- ----------------------------------------------------- ;~ ----------------------------------- ~ -- --------------------------- 03017x_

_

---- --- --------------------- y59 .084e . . . ------------ -------------- ---- ----

---------------------- ~-------- RZ= 0 .9455 ---- 1 0
~: . ------------------------------------------ --------

--------------------------------------------- ----- r -

----------------------------------------------- L ; -----------------------------------------------------

_____________________________________________________ O

----------------------------------------------------- 1 0 2 4 6 8

Depuration period (day ) 

### BHL = 2 .3 dayp . 24

Table 10-2 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak B ) WI W2 A B C DR D E depuration fish peak area conc . Dilution conc . residual period weight in final ratio in fish rat e

No .l No .2 count solution body day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 62 .1 100 .0 1 1 16 .65 9 .07 61845 32763 0 .00944 10 43 .4 73 .4 1 2 10 .19 11 .40 57190 32763 0 .00873 10 47 . 8 2 1 8 .06 16 .32 44232 31757 0 .00696 10 33 .8 59 .7 2 2 11 .38 11 .02 48644 31757 0 .00766 10 40 . 4 4 1 14 .47 8 .89 23602 21644 0 .00545 10 27 .6 32 .3 4 2 10 .67 10 .08 19003 21644 0 .00439 5 12 . 5 r ~ 8 1 12 .99 6 .63 53605 25894 0 .01035 1 6 .2 9 .8

8 2 11 .62 9 .98 ., 28584 25894 0 .00552 2 6
. 0 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : l OmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BXCstd

D=A=B xCstdxDRx(FV/1 000)x SF-, (W 1+W2)=(R/100)X 1000 E = (Di1+Di-,)-2=DoX 10 0 Figure 11-2 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak B )
100 --------------------------------------- - - - ------

------------------ ------ ---- ------ --------------------- y=62 .414e"o.zszX • =L

- Rz=0 .945 ----

------------------------- - - - - - - --- o • 10 ,~ ---------------------------------------------- ----

------------------------------------------------- : -------------------------------------------------- 4- ---------- -- ----------------------- ---------

------------

----------------------------------------------------

----------------------------------------------------
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - O 0 2 4 6 8 Depuration period (day) BHL = 2 .4 day 

## -6-a- t~~ '-Ap . 25

Table 10-3 Concentration of the test substance in the test fish during depuration tes t

- High concentration level (Peak C ) W1 W_, A B C DR D E depuration fish peak area conc . Dilution conc . residual period weight in final ratio in fish rat e

No .1 No .2 count solution body day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 44 .2 100 .0 1 1 16 .65 9 .07 49773 38267 0 .00650 10 29 .3 68 .9 1 2 10 .19 11 .40 45002 38267 0 .00588 10 31 . 6 2 1 8 .06 16 .32 31948 38496 0 .00415 10 19 .7 50 .5 2 2 11 .38 11 .02 37026 38496 0 .00481 10 24 . 9 4 1 14 .47 8 .89 16032 25618 0 .00313 10 15 .5 24 .9 4 2 10 .67 10 .08 11925 25618 0 .00233 5 6 . 5 8 1 12 .99 6 .63 27475 30239 0 .00454 1 2 .7 6 .1 8 2 11 .62 9 .98 15280 30239 0 .00253 2 2 .7 Concentration of std . solution (Cstd) : 0 .005rng/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=A=BXCst d

D =A=B XCstdxDRx(FV/1000)X SF=(W 1+W2)=(R/100)x 1000 E = (Di l+Di.))-2=Dox 100 Figure 11-3 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak C )

~ 100 ---------

------------------------------------------------------ -----------------------=------------------------------ n -------------------------------------y=43 .268e .3a9sX : : : : ~ --------------------------------- ----

----- ----------------------------- ---- v R2=0 .953 4

------------ ---------- --------- o ~ 10 ----- - - ---- - -- - --- -- -- ----- --- ,~ ------------------------- --------------------

-------------------------- ------------------ w -------------------- - - - -

---------------------------- ------ -- ------------- -- -- -------- ~ --------------------------------------------- -----

------------------------------

---------------- ~ ~ ------------------------------------------------------ 0 U 0 2 4 6 8 Depuration period (day) 

### BHL = 2 .0 day

we& 

## - to °~ Sp . 26

Table 10-4 Concentration of the test substance in the test fish during depuration tes t



### - High concentration level (Peak D) W, WZ A B C DR D E depuration fi sh peak area cone . Dilution cone . residual period weight in final ratio in fish rate

No .l No .2 count solution bod y day No . g g sample std . ing/L gg/g % 0 Concentration in fish body at steady state (28-60day) 42 .2 100 .0 I 1 16 .65 9 .07 51793 43233 0 .00599 10 27 .0 67 .2 1 2 10 .19 11 .40 47830 43233 0 .00553 10 29 . 7 2 1 8 .06 16 .32 33016 42807 0 .00386 10 18 .3 48 .8 2 2 11 .38 11 .02 37819 42807 0 .00442 10 22 . 9 4 1 14 .47 8 .89 15931 28766 0 .00277 10 13 .7 22 .9 ~ 4 2 10 .67 10 .08 11611 28766 '0 .00202 5 5 . 6

8 1 12 .99 6 .63 24748 34439 0 .00359 1 2 .1 5 .1 8 2 11 .62 9 .98 14267 34439 0 .00207 2 2 .2 Concentration of std . solution ( Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor ( SF) : 1000 Recove ry (R) : 86 .3% Equations : C=A=BxCstd

D =A=BxCstdXDRX(FV/1000)xSF=(W 1+W2)=(R/100)x 1000 • E=(Di l +Di2)-2=Dox100 Figure 11-4 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak D) ^ 100 --------- - - - - - - - - - ---- -

-----------------------------------------------------

------------------------------------------------------ 

# I ------

-------------- -



# ---------------------------------

-- --------- ------
0 2 4 6 8 Deputation period (day) 

### BHL = 1 .9 dayp . 27

Table 11-1 Concentration of the test substance in the test fish during depuration tes t

- Low concentration level (Peak A ) W, W2 A B C DR D E depuration fish peak area conc . Dilution conc . residual period weight in final ratio in fish rat e

No .l No .2 count solution bod y day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 6 .50 100 .0 1 1 15 .07 7 .75 40070 32770 0 .00611 1 3 .17 52 .8 1 2 10 .94 11 .27 45433 32770 0 .00693 1 3 .6 9 2 1 12 .88 8 .04 38505 31335 0 .00614 1 3 .47 54 .8 2 2 11 .70 13 .97 49685 31335 0 .00793 1 3 .6 5 4 1 16 .05 12 .24 16915 21570 0 .00392 1 1 .64 30 .1 4 2 13 .32 17 .16 25219 21570 0 .00585 1 2 .2 7 8 1 13 .88 13 .21 7820 25485 0 .00153 1 0 .67 14 .6 8 2 12 .18 11 .36 12466 25485 0 .00245 1 1 .23 Concentration of std . solution (Cstd) : 0 .005rng/ L Final volume (FV) : 10mL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCstd

D=A=B x CstdXDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000 E = (D i l+DiZ)-2=Do x 10 0 Figure 12-1 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak A) 

### 10

------------------------------------------------------ --------------------

-----------------

----------------- -------y5 .0568e-0zi99X---==

---------------------------------- ---- ---- ---------------- ------ 

# =L -------------------- R 0 .8931 ---- ------ ------- --------------------------- ----------------------- ~ _---------------------------------------------------- w

------------------------------------------------------

----------------------------------------------------

------------------------------------------------------

------------------------------------------------------ 0-1 0 2 4 6 8 Depuration period (day ) 

### BHL = 3 .2 dayp . 28

Table 11-2 Concentration of the test substance in the test fish during depuration tes t



### - Low concentration level (Peak B ) W , W2 A B C DR D E depuration fish peak area conc . Dilution conc . residual period weight in final ratio in fish rate

No .l No .2 count solution body day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 6 .84 100 .0 1 1 15 .07 7 .75 42873 32763 0 .00654 1 3 .39 53 .7 1 2 10 .94 11 .27 48620 32763 0 .00742 1 3 .9 5 2 1 12 .88 8 .04 41682 31757 0 .00656 1 3 .71 55 .5 2 2 11 .70 13 .97 53487 31757 0 .00842 1 3 .8 8 4 1 16 .05 12 .24 18713 21644 0 .00432 1 1 .81 31 .4 4 2 13 .32 17 .16 27725 21644 0 .00640 1 2 .4 8 8 1 13 .88 13 .21 8852 . 25894 0 .00171 1 0 .75 15 .5 8 2 12 .18 11 .36 14084 25894 0 .00272 1 1 .37 Concentration of std . solution (Cstd) : 0 .005mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 84 .6% Equations : C=A=BxCst d

D =A=BxCstdxDRx(FV/ 1000)x SF=(W 1+W2)=(R/100)x 1000 E=(Di1+Di2)+2=Dox 100 Figure 12-2 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak B )

10 ----- ----------- -- -- - - -- - - - ,- . ------ - ----------------------------------- \en ---------- ozi~~----

----------------- ----Y=53506e-- ------ •- ------- =L ----- --------
----------------- RZ0 .891

-------------------------- ~-- -------------------- 41 - - - - - - - - - - - - = : - - - - - - - Z : : : : - - - - - --- - - ------ ----- - - - 2 : :7 - -- - - - - - - Z : : - - - ----------- --------- ------------------------ ~ ----------------------------
---------------------------------- -------
-------------------------------- o -------------------- . . . ---------------------------------------------------- !

----------------------------------------------------

---------------------------------------------------- U 0 .1 0 2 4 6 8 Depuration period (day ) BHL = 3 .3 dayp . 29

Table 11-3 Concentration of the test substance in the test fish during depuration tes t



### - Low concentration level (Peak C ) W, W2 A B C DR D E depuration fish peak area conc . Dilution conc . residual period weight in final ratio in fish rate

No .1 No .2 count solution body day No. g g sample std . mg/L gg/g % 0 Concentration in fish body at steady state (28-60day) 4 .81 100 .0 1 1 15 .07 7 .75 33730 38267 0 .00441 1 2 .24 50 .5 1 2 10 .94 11 .27 38405 38267 0 .00502 1 2 .62 2 1 12 .88 8 .04 30674 38496 0 .00398 1 2 .21 47 .3 2 2 11 .70 13 .97 39954 38496 0 .00519 1 2 .3 4 4 1 16 .05 12 .24 12670 25618 0 .00247 1 1 .01 24 .8 4 2 13 .32 17 .16 18584 25618 0 .00363 1 1 .3 8 8 1 13 .88 13 .21 5027 30239 0 .00083 1 0 .36 10 .2 8 2 12 .18 11 .36 7653 30239 0 .00127 1 0 .62 Concentration of std . solution ( Cstd) : 0 .005rng/L Final volume (FV) : lOmL Sampling factor ( SF) : 1000 Recovery (R) : 86 .3% Equations : C=A=BxCst d

D =A=BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)X 1000 E= (Di l+Di2)-2=Dax 10 0 Figure 12-3 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak C ) 

### 10

-

- y 3 

### --

- 

### -- ------

-



### -

- 

### - - - - - - - - - - - - - - - - - - - - 0 2 619x

------------------- =--Y=3 .6582e ' en R2= 0 .9314

-- ---------------------------------------- ` . ------ ------- -- -------------------------------------- 0 1 ----- --- -----

--

----------- ---------------------------------------------

. . . ----- ------ w~ -------------------- ----- ------------------------------ G -------------------- ----------------------------------

-----------------------------------------------------------

------------------------------ ---------------------------- 0 U 0 .1 0 2 4 6 8 Depuration period (day) 

### BHL = 2 .6 dayp . 30

Table 11-4 Concentration of the test substance in the test fish during depuration test



### - Low concentration level (Peak D) Wl WZ A B C DR D E depuration fish peak area cone . Dilution cone . residual period weight in final ratio in fish rate

No .1 No .2 count solution bod y day No . g g sample std . mg/L μg/g % 0 Concentration in fish body at steady state (28-60day) 4 .57 100 .0 1 1 15 .07 7 .75 35294 43233 0 .00408 1 2 .07 49 .2 1 2 10 .94 11 .27 40246 43233 0 .00465 1 2 .4 3 2 1 12 .88 8 .04 31492 42807 0 .00368 1 2 .04 45 .8 2 2 11 .70 13 .97 40731 42807 0 .00476 1 2 .1 5 4 1 16 .05 12 .24 12723 28766 0 .00221 1 0 .91 23 .5 4 2 13 .32 17 .16 18807 28766 0 .00327 1 1 .24 8 1 13 .88 13 .21 5029 34439 0 .00073 1 0 .31 9 .3 8 2 12 .18 11 .36 7552 34439 0 .00110 1 0 .54 Concentration of std . solution (Cstd) : 0 .005 mg/L Final volume (FV) : lOmL Sampling factor (SF) : 1000 Recovery (R) : 86 .3% Equations : C=A=BxCstd

D =A=B x CstdxDRx(FV/ 1000)X SF=(W 1+W2)=(R/100)X 1000 E=(Di1+Di2)-2=DoX 10 0 Figure 12-4 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak D)
10 

### -------------------------------------------------------

--- ----------- ------------------------- - --- ------------------------------------- -----

------------------- ------------------ -3 .4395e-o2rz4X y = -

----- -- ---------------------------------- ~ R2 _0 .9336- ----- 0 .fl ~ 1 -------------------------- ------- ------------------- ~ ------------- v~ --------------------------------------- ------------- 4 ; ---- - - ------------------------------
--------------------------------- - - -

- - -
----- -- - - -
-

- - - - :11 - - - -

---------------------------------------------------- V _ _ _ _ _ _ _ _

_

--------------------------------------------- G 0 ------------------------------------------------------- U 0 .1 0 2 4 6 Deputation period (day) 

### BHI., = 2 .5 day 

### -6}- ~ o Op . 31

Figure 1 Bioconcentration test system tap water 

## IF " air pump pump - [Lr 0 0 0 feed solution dilution wate r (24±2°C, D .O .>7mg/L) activated charcoal (dechlorination) sodium mixing tube thiosulfate solution ~ te flon sheet 56L-glass aquarium (50L water)
to waste treatment system (Apparatus ) Pump (dilution water supply)

Exposure : FMI Co ., QD-2CSC Control : FMI Co ., QD-2CSC ~ Pump (feed solution supply ) Exposure : Nihon Seimitsu Kagaku Co ., SP-D-2501(S) Control : Nihon Seimitsu Kagaku Co ., SP-T-2500 S



# ~~~~p . 32

Figure 2-1 Calibration curve of the test substance (Peak A ) Input Data No . Concentration Peak Area

(mg/L) (count) 1 0 0 2 0 .0025 20496 3 0 .005 41273 4 0 .01 78797 Y= 7,970,OOOX r= 0 .9995 detection limit = 0 .00025mg/ L
80000 S

~ 60000

C ~ . )
~ . . i Sud 40000 !R'

~ 20000 4V

0 ~ 0 0 .0025 0 .005 0 .0075 0 .01 Concentration (mg/L)p . 33

Figure 2-2 Calibratiori curve of the test substance (Peak B ) Input Data No . Concentration Peak Area

(mg/L) (count) 1 0 0 2 0 .0025 20847 3 0 .005 42104 4 0 .01 79471 Y= 8,060,OOOX r= 0 .9993 detection limit = 0 .00025mg/L

80000 .

60000
~ _. . 0 v c,d 40000 S .
20000

0 l 0 0 .0025 0 .005 0 .0075 0 .0 1 t Concentration (mg/L)p . 34

Figure 2-3 Calibration curve of the test substance (Peak C ) Input Data No . Concentration Peak Area

(mg/L) (count) 1 0 0 2 0 .0025 24313 3 0 .005 49713 4 0 .01 96272 Y= 9,690,OOOX r= 0 .9998 detection limit = 0 .00025mg/L

100000

~ 80000
60000 - 0 lt~ a~i •' 40000
~, . 2000 0
0 t 0 0 .0025 0 .005 0 .0075 0 .01 Concentration (mg/L)p . 35

Figure 2-4 Calibration curve of the test substance (Peak D ) Input Data No . Concentration Peak Area

(mg/L) (count) 1 0 0 2 0 .0025 27773 3 0 .005 56335 4 0 .01 108311 Y= 10,930,000X 

### r= 0 .9997 detection limit = 0 .00025mg/L
~ 100000 

## 80000
60000 N ~ I . Cd 40000 ~, . 2000 0
0 ~ 0 0 .0025 0 .005 0 .0075 0 .01 Concentration (mg/L)p . 36



## S

# ANITIZEDp . 37



# Appendix 1

Information on the test substance

(2 pages)p . 38

1• ._ ~^e-~ ~ Lc~ ~-b s o,~ ~~ ~ o,r~ S e c~,- ~



## SAN ITIZED

k 3p . 39



# Appendix 2

~ Dilution water quality (2 pages )

tp . 40

Dilution water quality

Parameter Concentration BOD <2 .0 mg/L COD <3 .0 mg/L PH 8 .0 (20°C) Coliform group bacteria N
.D Oil N .D Cadmium <0 .01 mg/L Cyanide N .D Lead <0 .1 mg/L Chromium <0 .05 mg/L Arsenic <0 .05 mg/L Mercury <0 .0005 mg/L Free chlorine <0 .02 mg/L Bromide < 1 .0 mg/L Fluoride <1 .5 mg/L Sulfide <0 .3 mg/L Total ammonium <1 .0 mg/L Copper <0 .005 mg/L Zinc <0 .1 mg/L Aluminum <0 .1 mg/L Tin <1 .0 mg/L Manganese <1 .0 mg/L Iron <1 .0 mg/L Nickel <0 .1 mg/L Total phosphorus <0 .1 mg/L f . Selenium <0 .001 mg/L Phenols <0 .002 mg/L Anionic surfactant <0 .02 mg/L Evaporation residue 88 mg/L Electric conductivity 131 ~c S/cm Total hardness (as CaCO3) 49 mg/L Alkalinity 29 mg/L Sodium 8 .8 mg/L Potassium 1 .1 mg/L Calcium 14 mg/L Magnesium 3 .6 mg/L 

### sampling date : February 6, 2006p . 41



# Appendix 3
Chromatograms (Analysis of the test substance in the test water) (10 pages)

~-~`~p . 42

C- V\~..r o V "

\,~ o vo`w` S 

## SANITIZEDp . 43



# Appendix 4
Chromatograms (Analysis of the test substance in the test fish during exposure period) (11 pages)

(



# 2 ~ -4-7p . 44



## S

# ANITIZEDp . 45



# Appendix 5
Chromatograms (Analysis of the test substance in the tissues) (5 pages )

~



### } 

## ~ Z ~~p . 46



## SANITIZE D



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# ~p . 47



# Appendix
. 6
Chromatograms (Analysis of the test substance in the test fish during depuration period) (9 pages)

~



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