# Patent

## Title
Heteroarylamine derivatives as protein kinase inhibitors

## Abstract

A heteroarylamine compound includes protein kinase inhibition activity, a pharmaceutically acceptable salt thereof and a pharmaceutical composition for preventing and treating a disease caused by abnormal cell growth, which contains the compound as an active ingredient.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims, under 35 U.S.C. §119, the priority of Korean Patent Application No. 10-2014-0166484, filed on Nov. 26, 2014, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein by reference in its entirety.
BACKGROUND
(a) Technical Field
The present invention relates to a novel heteroarylamine derivative having an effect of inhibiting a protein kinase and a pharmaceutical composition for preventing and treating cancer induced by abnormal cell growth, which contains the novel compound as an active ingredient.
(b) Background Art
Fibroblast growth factors (FGFs) and their receptors regulate various biological development processes including tissue regeneration, hematopoiesis, angiogenesis, embryonic development, etc. The FGF receptors (FGFRs) are divided into 4 subtypes which share extracellular ligand-binding domains, hydrophobic transmembrane domains and intracellular kinase domains. FGFR dimerization occurs when an FGF ligand is bound to the FGFR. Subsequently, the activation of downstream signaling pathways is induced by autophosphorylation and the above-described various biological development processes are regulated.
Recently, FGFs and FGFRs are drawing attentions as oncogenes acting as major proliferation factors in cancer cells resistant to various anticancer drugs. Abnormal FGFR-mediated signal transduction induces breast cancer, multiple myeloma, bladder cancer, endometrial cancer, stomach cancer, prostate cancer, etc. The carcinogenesis of various cancers associated with FGFR is caused by the overexpression of the receptor due to proliferation of the gene and abnormal transcriptional regulation. As a result, the cancer cells acquire high sensitivity to the FGF ligand or proliferate via a mechanism of autoactivation through dimerization independently of the ligand.
The inhibition of the FGF/FGFR signal transduction system for anticancer therapy is very important in the development of kinase-based anticancer drugs. At present, a number of compounds (e.g., BIBF-1120, SU6668, NVP-BGJ398, AZD4547) are under clinical trials. Because these compounds are FGFR inhibitors with poor selectivity, it is difficult to pharmacologically identify the anticancer mechanism through inhibition of FGFR. Accordingly, a novel FGFR inhibitor with good selectivity and high activity needs to be developed.
J. Med. Chem. 2011, 54, 7066-7083 discloses that the compound 3-(2,6-dichloro-3,5-dimethoxyphenyl)-1-{6-[4-(4-ethyl-piperazin-1-yl)phenylamino]pyrimidin-4-yl}-1-methylurea (NVP-BGJ398) has selective inhibition activity against FGFR3 and exhibits effective anticancer activity for the RT112 bladder cancer cell line.




Until now, several compounds having benz[d]imidazol-2-amine as a backbone have been synthesized. But, the heteroarylamine compound of the present invention has never been reported. In addition, the inhibition activity of the heteroarylamine compound of the present invention against a protein kinase and its use for treatment and prevention of tumors have not been predicted yet.
REFERENCES OF THE RELATED ART
Non-Patent Document


    (Non-patent document 1) J. Med. Chem. 2011, 54, 7066-7083.


SUMMARY
The present invention is directed to providing a novel compound having specific substituents for a heteroarylamine backbone or a pharmaceutically acceptable salt thereof.
The present invention is also directed to providing a pharmaceutical composition for preventing and treating a disease caused by abnormal cell growth, which contains the novel compound or a pharmaceutically acceptable salt thereof as an active ingredient.
The present invention is also directed to providing a method for preparing the novel compound.
The present invention is also directed to providing a novel intermediate compound synthesized during the process of the above-described preparation method.
In an aspect, the present invention provides a compound selected from a heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a hydrate thereof and a solvate thereof.




In Chemical Formula 1,
each of X1, X2, X3 and X4 is N or CH,
R1 is one to four substituent(s) selected from a halogen atom, C1-C8 alkyl, C1-C8 alkoxy and




R2 is a hydrogen atom, C1-C8 alkyl, —(CH2)m—NR3R4 or -A-(CH2)n-(Q)p-R5,
A is C6-C15 aryl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) or 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein each of the aryl, heteroaryl or heterocycloalkyl may be substituted or unsubstituted with one to three substituent(s) selected from C1-C8 alkyl, C1-C8 alkoxy, C1-C8 haloalkyl and C1-C8 alkoxycarbonyl,
L is —NHC(O)— or —C(O)NH—,
Z is one to four substituent(s) selected from C1-C8 alkyl, C1-C8 haloalkyl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) and 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein the heteroaryl or heterocycloalkyl may be substituted or unsubstituted with C1-C8 alkyl,
Q is 5- or 6-membered heterocycloalkyl or biheterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom,
each of R3 and R4 is a hydrogen atom or C1-C8 alkyl,
R5 is a hydrogen atom, C(O)OR7 (wherein R7 is a hydrogen atom or C1-C8 alkyl) or C1-C8 alkyl,
m is an integer from 1 to 6,
n is an integer from 0 to 6, and
p, which indicates the presence or absence of Q, is 0 or 1.
Since the compound of the present invention exhibits a superior effect of inhibiting the activity of a protein kinase selected from ABL2, ACK1, ALK, BLK, BMX, c-Kit, c-Src, DDR1, DDR2, DYRK1, DYRK1B, DYRK2, EGFR, HER2, FGFR1, FGFR2, FGFR3, FGFR4, FGF, FLT3, FLT4, FMS, Fyn, Hck, MAP4K5, LCK, LIMK1, LYN, LYN B, MEKK2, MEKK3, ROS, SIK1, SIK2, SIK3, TAOK1, TAOK2, TIE2, TNK1, TRKA and YES, it can be used as an active ingredient of a pharmaceutical composition for preventing and treating a disease caused by abnormal cell growth.
Accordingly, the compound according to the present invention can be used for preventing and treating diseases caused by abnormal cell growth, for example, various tumor diseases selected from endometrial cancer, bladder cancer, stomach cancer, lung cancer, liver cancer, colon cancer, small intestine cancer, pancreatic cancer, brain cancer, bone cancer, melanoma, breast cancer, sclerosing adenosis, head and neck cancer, esophageal cancer, thyroid cancer, parathyroid cancer, kidney cancer, sarcoma, prostate cancer, urethral cancer, blood cancer such as leukemia, multiple myeloma and myelodysplastic syndrome, lymphoma such as Hodgkin's disease and non-Hodgkin lymphoma, fibroadenoma, etc.



BRIEF DESCRIPTION OF DRAWING
FIG. 1 shows a result of evaluating anticancer effect in an endometrial cancer cell line-transplanted animal model.



DETAILED DESCRIPTION
A pharmaceutically acceptable salt of a heteroarylamine compound represented by Chemical Formula 1 according to the present invention can be prepared by a method commonly employed in the related art. The pharmaceutically acceptable salt should have low toxicity in human body and should not negatively affect the biological activity and physicochemical properties of the mother compound. A free acid that may be used to prepare the pharmaceutically acceptable salt may be either an inorganic acid or an organic acid. The inorganic acid may be hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, perchloric acid, bromic acid, etc. The organic acid may be acetic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, fumaric acid, maleic acid, malonic acid, phthalic acid, succinic acid, lactic acid, citric acid, gluconic acid, tartaric acid, salicylic acid, malic acid, oxalic acid, benzoic acid, embonic acid, aspartic acid, glutamic acid, etc. An organic base that may be used to prepare an organic base addition salt includes tris(hydroxymethyl)methylamine, dicyclohexylamine, etc. An amino acid that may be used to prepare an amino acid addition salt includes a natural amino acid such as alanine, glycine, etc.
The heteroarylamine compound represented by Chemical Formula 1 according to the present invention includes, in addition to the above-described pharmaceutically acceptable salts, all hydrates and solvates. The hydrate and solvate may be prepared by dissolving the heteroarylamine compound represented by Chemical Formula 1 in a water-miscible solvent such as methanol, ethanol, acetone and 1,4-dioxane, and conducting crystallization or recrystallization after adding a free acid or a free base. The compound of the present invention also includes, in addition to the compounds containing various amounts of water that can be prepared, for example, through lyophilization, stoichiometric solvates including hydrates.
Hereinafter, the substituents used to define the compound according to the present invention are described in detail.
In the present invention, a ‘halogen atom’ refers to chloro, fluoro, bromo or iodo.
In the present invention, ‘alkyl’ refers to a linear, branched or cyclic aliphatic saturated hydrocarbon group having one to eight carbon atom(s), including methyl, ethyl, n-propyl, i-propyl, cyclopropyl, n-butyl, i-butyl, t-butyl, cyclobutyl, cyclopropylmethyl, n-pentyl, i-pentyl, neopentyl, t-pentyl, cyclopentyl, cyclobutylmethyl, n-hexyl, i-hexyl, cyclohexyl, cyclopentylmethyl, heptyl, cyclohexylmethyl, octyl, etc.
In the present invention, ‘haloalkyl’ refers to an alkyl group having one or more hydrogen atom substituted with a halogen atom, such as trifluoromethyl.
In the present invention, ‘alkoxy’ refers to a hydroxyl group having a hydrogen atom substituted with a C1-C8 alkyl group, including methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy and t-butoxy.
In the present invention, ‘aryl’ refers to a monocyclic, bicyclic or tricyclic aromatic hydrocarbon group having six to fifteen carbon atom(s), including phenyl, naphthyl, anthryl, phenanthryl, etc.
In the present invention, ‘heteroaryl’ refers to a monocyclic, bicyclic or tricyclic aromatic heterohydrocarbon group containing one or more heteroatom selected from N, O and S, including pyrrolyl, furanyl, thiophenyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridinyl, pyrazinyl, pyridazinyl, pyrimidinyl, indolyl, isoindolyl, benzofuranyl, benzofurazanyl, dibenzofuranyl, isobenzofuranyl, indazolyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, dibenzothiophenyl, naphthyridinyl, benzisothiazolyl, quinolinyl, isoquinolinyl, quinoxalinyl, phthalazinyl, quinolinyl, quinazolinyl, etc.
In the present invention, ‘heterocycloalkyl’ refers to a 5- or 6-membered aliphatic heterohydrocarbon cyclic group containing one or more heteroatom selected from N and O, including morpholinyl, piperidinyl, piperazinyl, N-protected piperazinyl, etc. In general, an N-protecting group of the piperazinyl may include an alkyl group.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein X1, X2, X3 and X4 are CH at the same time.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein X1 and X4 are N and X2 and X3 are CH.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein X1, X3 and X4 are CH and X2 is N.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R1, which is a substituent substituted at the phenyl ring, is one to four substituent(s) selected from chloro, methyl, methoxy, —NHC(O)-(3-trifluoromethylbenzene) and —C(O)NH-(3-trifluoromethylbenzene).
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R2 is a hydrogen atom.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R2 is methyl, ethyl or cyclopropyl.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R2 is 2-(dimethylamino)ethyl or 3-(dimethylamino)propyl.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R2 is phenyl, methoxy-substituted phenyl, trifluoromethyl-substituted phenyl, methoxy- and methoxycarbonyl-substituted phenyl, pyridinyl, pyrimidinyl, 4-methylpiperidin-1-yl or 4-ethylpiperidin-1-yl.
Specifically, the compound represented by Chemical Formula 1 according to the present invention may be a compound wherein R2 is




(wherein each of X and Y, which are identical to or different from each other, is CH or N, Q is piperidinyl, bipiperidinyl, piperazinyl or morpholino, R5 is a hydrogen atom, methyl, ethyl, cyclohexylmethyl or methoxycarbonyl, R6 is a hydrogen atom, methoxy, trifluoromethyl or methoxycarbonyl and n is an integer from 0 to 3).

Specific examples of the compound represented by Chemical Formula 1 according to the present invention are as follows:

    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((2-methoxy-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-morpholinophenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((2-methoxy-4-morpholinophenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    1-(6-((4-([1,4′-bipiperidin]-1′-yl)-3-(trifluoromethyl)phenyl)amino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine;
    1-(6-((4-((4-(cyclohexylmethyl)piperazin-1-yl)methyl)phenyl)amino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine;
    methyl 4-((6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-4-yl)amino)-3-methoxybenzoate;
    1-(6-aminopyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(methylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(phenylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(pyrimidin-2-ylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((6-(4-ethylpiperazin-1-yl)pyrimidin-4-yl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((1-methylpiperidin-4-yl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N1-(6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-4-yl)-N2,N2-dimethylethane-1,2-diamine;
    N1-(6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-4-yl)-N3,N3-dimethylpropane-1,3-diamine;
    1-(6-(cyclopropylamino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine;
    N-(3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine;
    N-(3,5-dimethoxyphenyl)-3-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-amine;
    N-(3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-amine;
    N-(3-((3-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide;
    N-(3-((1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide; and
    4-methyl-N-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenyl)-3-((3-(6-(phenylamino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)benzamide.


The present invention also provides a method for preparing the compound represented by Chemical Formula 1.
The preparation method of the present invention, which is represented by Scheme 1, includes:
i) a process of preparing a compound represented by Chemical Formula 4 by coupling a compound represented by Chemical Formula 2 with a compound represented by Chemical Formula 3; and
ii) a process of preparing a heteroarylamine compound represented by Chemical Formula 1 by reacting the compound represented by Chemical Formula 4 with an a amine compound represented by Chemical Formula 5 having various functional groups.




In Scheme 1, X1, X2, X3, X4, R1 and R2 are the same as defined above.
In the preparation method according to Scheme 1, the process i) is conducted by heating to 60-110° C. in the presence of an alkali metal salt as an inorganic base selected from an alkali metal hydride, an alkali metal hydroxide, etc. As a reaction solvent, a common organic solvent such as tetrahydrofuran, dioxane, N,N-dimethylformamide, N,N-dimethyl sulfoxide, 2-butanol, 2-pentanol, etc. may be used.
And, the process ii) is conducted via amination at high temperature in the presence of an acid or a base or via Buckwald amination using an organometal compound. In the Buckwald amination, Pd2(dba)3, Pd(OAc)2, PdCl2(PPh3)2, Pd(PPh3)4, etc. may be used as the metal compound. As a ligand, Xantphos (CAS number: 161265-03-8), Davephos (CAS number: 213697-53-1), Johnphos (CAS number: 224311-51-7), X-phos (CAS number: 564483-18-7), tert-Butyl Xphos (CAS number: 564483-19-8), etc. may be used. And, as the base, an amine-based organic base or a carbonate, sulfate, phosphate, alkoxide, etc. of an alkali metal or an alkaline earth metal may be used. As a reaction solvent, a common organic solvent such as tetrahydrofuran, dioxane, N,N-dimethylformamide, N,N-dimethyl sulfoxide, 2-butanol, 2-pentanol, etc. may be used. Reaction temperature may be maintained at 50-200° C., specifically 80-150° C.
The compound represented by Chemical Formula 2 and the compound represented by Chemical Formula 4, which are synthesized as reaction intermediates in the preparation method according to Scheme 1, are novel compounds first synthesized in the present invention. Accordingly, the Chemical Formula 2 or the compound represented by Chemical Formula 4 as intermediate compounds is included in the scope of the present invention.
The compound represented by Chemical Formula 2, which is used as a starting material in preparation method according to Scheme 1, may be prepared by a common organic synthesis method using an aniline derivative as a source material, as described in Scheme 2.




In Scheme 2, X1, X2, X3, X4 and R1 are the same as defined above.
In the preparation method according to Scheme 2, first, an amine group of an aniline derivative represented by Chemical Formula 6 is converted to an isothiocyanate (NCS) group by slowly adding thiophosgene (CSCl2) dropwise in a mixture solvent of dichloromethane and water. Then, a benz[d]imidazol-2-amine backbone is prepared by reacting an isothiocyanatobenzene represented by Chemical Formula 7 with a benzene-1,2-diamine represented by Chemical Formula 8. In this process, the compound represented by Chemical Formula 2 is prepared using the amidation reagent 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) by stirring in a dichloromethane solvent.
A pharmaceutical composition containing the heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a solvate thereof or a hydrate thereof as an active ingredient are included in the scope of rights of the present invention.
The heteroarylamine compound represented by Chemical Formula 1 exhibits inhibition activity against various protein kinases. In particular, because it exhibits superior inhibition activity against ABL2, ACK1, ALK, BLK, BMX, c-Kit, c-Src, DDR1, DDR2, DYRK1, DYRK1B, DYRK2, EGFR, HER2, FGFR1, FGFR2, FGFR3, FGFR4, FGF, FLT3, FLT4, FMS, Fyn, Hck, MAP4K5, LCK, LIMK1, LYN, LYN B, MEKK2, MEKK3, ROS, SIK1, SIK2, SIK3, TAOK1, TAOK2, TIE2, TNK1, TRKA and YES, the pharmaceutical composition of the present invention may be used to prevent or treat a disease caused by abnormal cell growth. Specifically, the disease caused by abnormal cell growth may include various tumor diseases such as endometrial cancer, bladder cancer, stomach cancer, lung cancer, liver cancer, colon cancer, small intestine cancer, pancreatic cancer, brain cancer, bone cancer, melanoma, breast cancer, sclerosing adenosis, head and neck cancer, esophageal cancer, thyroid cancer, parathyroid cancer, kidney cancer, sarcoma, prostate cancer, urethral cancer, blood cancer such as leukemia, multiple myeloma and myelodysplastic syndrome, lymphoma such as Hodgkin's disease and non-Hodgkin lymphoma, fibroadenoma, etc.
Accordingly, the present invention provides a pharmaceutical composition containing the heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a solvate thereof or a hydrate thereof as an active ingredient, and an agent for preventing and treating various tumor diseases caused by abnormal cell growth.
The pharmaceutical composition of the present invention containing to one or more active ingredient selected from the heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a solvate and a hydrate thereof may be prepared into a formulation for oral or parenteral administration common in the pharmaceutical field, e.g., a tablet, a capsule, a troche, a liquid, a suspension, etc., by adding a commonly used nontoxic, pharmaceutically acceptable carrier, adjuvant, excipient, etc.
The excipient that may be used in the pharmaceutical composition of the present invention may include a sweetener, a binder, a solvent, a solubilizer, a wetting agent, an emulsifier, an isotonic agent, an adsorbent, a disintegrant, an antioxidant, an antiseptic, a lubricant, a filler, an aromatic, etc. Examples may include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, silica, talc, stearic acid, stearin, magnesium stearate, magnesium aluminum silicate, starch, gelatin, gum tragacanth, alginic acid, sodium alginate, methyl cellulose, sodium carboxymethyl cellulose, agar, water, ethanol, polyethylene glycol, polyvinylpyrrolidone, sodium chloride, calcium chloride, orange essence, strawberry essence, vanilla flavor, etc.
An administration dosage of the compound according to the present invention to human may vary depending on the age, body weight and sex of the patient, administration type, health condition and severity of disease. A general dosage for an adult patient weighing 70 kg is 0.01-1,000 mg/day. The pharmaceutical composition may be administered once a day or several times a day with predetermined intervals at the discretion of a physician or a pharmacist.
EXAMPLES
The present invention will be described in more detail through examples, formulation examples and test examples. The following examples, formulation examples and test examples are for illustrative purposes only and it will be apparent to those skilled in the art that the scope of this invention is not limited by them.
Examples
Example 1
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A compound of Example 1 having the above structural formula was prepared as follows.
Step 1: N-(3,5-dimethoxyphenyl)acetamide




After slowly adding acetic anhydride (63 mL, 653.3 mmol) dropwise for 15 minutes to a solution of 3,5-dimethoxyaniline (95 g, 622.2 mmol) in toluene (500 mL), the mixture was stirred at 35-45° C. for 20 hours. The produced gray suspended matter was diluted by adding hexane (200 mL) and the produced precipitate was obtained by filtering. The resulting solid was washed with toluene/hexane (2:1, 200 mL) and hexane (100 mL) and dried to obtain a target compound (110 g, 91% yield) as a colorless solid. MS m/z: 196 [M+1]+.
Step 2: N-(2,6-dichloro-3,5-dimethoxyphenyl)acetamide




A round-bottom flask was filled with argon gas after adding N-(3,5-dimethoxyphenyl)acetamide (32.9 g, 220 mmol). After dissolving the reactant in acetonitrile (800 mL), sulfuryl chloride (SO2Cl2, 35 mL, 440 mmol) was slowly added at 0° C. dropwise for 15 minutes. The reaction mixture was stirred at 0° C. for 30 minutes and reaction was terminated by slowly adding a saturated sodium bicarbonate solution (500 mL) dropwise. Then, after extracting several times using ethyl acetate, the organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The resulting solution was purified by chromatography (ethyl acetate/hexane, 1:1→2:1) to obtain a target compound (34.5 g, 60% yield) as a colorless solid. 1H NMR (400 MHz, DMSO-d6) δ 9.74 (s, 1H), 6.88 (s, 1H), 3.92 (s, 6H), 2.03 (s, 3H).
Step 3: 2,6-dichloro-3,5-dimethoxyaniline




After adding a 2 N potassium hydroxide aqueous solution (400 mL) to a solution of N-(2,6-dichloro-3,5-dimethoxyphenyl)acetamide (34.5 g, 131 mmol) in ethanol (600 mL), the reaction mixture was stirred at 90° C. for 2 days under reflux. The mixture solution was cooled to room temperature and stirred again for 1 hour after cooling to 0° C. using ice water. The produced solid was filtered, washed with a cold ethanol/water (1:1) mixture solvent and then dried to obtain a target compound (22.1 g, 76% yield). 1H NMR (400 MHz, DMSO-d6) δ 6.21 (s, 1H), 5.40 (s, 2H), 3.83 (s, 6H).
Step 4: 2,4-dichloro-3-isothiocyanato-1,5-dimethoxybenzene




After adding 2,6-dichloro-3,5-dimethoxyaniline (10.1 g, 45 mmol) to a mixture solvent of dichloromethane (80 mL) and water (30 mL) and slowly adding thiophosgene (10 mL) dropwise for 20 minutes, the reaction mixture was stirred for 2 days. Then, after extracting several times using dichloromethane, the organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated to obtain a target compound (13.5 g) as a yellow solid.
Step 5: N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-3-amine




After adding a solution of the 2,4-dichloro-3-isothiocyanato-1,5-dimethoxybenzene (13.5 g, 45 mmol) obtained in the step 4 in dichloromethane (150 mL) to benzene-1,2-diamine (9.7 g, 90 mmol), the mixture was stirred for 12 hours. Then, after adding EDCI (17.3 g, 90 mmol), the reaction mixture was further stirred for 12 hours. The reaction mixture was concentrated without special purification and then purified by column chromatography (ethyl acetate/hexane, 1:1→dichloromethane/methanol, 10:1) to obtain a target compound (10 g, 66% yield) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ 12.10 (brs, 1H), 7.30 (m, 2H), 7.20 (m, 2H), 7.06 (s, 1H), 3.99 (s, 6H). MS m/z: 338 [M+1]+.
Step 6: 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine




N-(2,6-Dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-3-amine (45 mg, 0.108 mmol) and 4,6-dichloropyrimidine (40 mg, 0.27 mmol) were dissolved in dimethyl sulfoxide (DMSO, 0.4 mL) and the mixture was cooled to 0° C. After slowly adding 60% sodium hydride (6.6 mg, 0.165 mmol) and heating to 90° C., the mixture was stirred for 3 hours. Then, after cooling to room temperature, reaction was terminated by adding water. After extracting several times using ethyl acetate, the resulting organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The obtained residue was washed four times with acetonitrile (8 mL) and the solvent was removed to obtain a target compound (24 mg, 42% yield). 1H NMR (400 MHz, DMSO-d6) δ 10.97 (brs, 1H) 9.07 (s, 1H), 8.99 (s, 1H), 8.33 (d, J=7.6 Hz, 1H), 7.14 (m, 1H) 7.08 (m, 1H), 6.98 (d, J=8 Hz, 1H), 6.70 (s, 1H), 3.93 (s, 6H). MS m/z: 451 [M+1]+.
Step 7: N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




After dissolving 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine (20 mg, 0.044 mmol) and 4-(4-ethylpiperazin-1-yl)aniline (13.6 mg, 0.066 mmol) in a mixture of 1,4-dioxane (0.1 mL) and dimethyl sulfoxide (0.1 mL) and cooling to 0° C., trifluoroacetic acid (10.2 μL, 0.133 mmol) was slowly added dropwise. Then, after heating to 120° C., the reaction mixture was stirred for 20 hours. After the reaction was terminated, the reaction mixture was cooled to room temperature. After extracting several times using ethyl acetate, the obtained organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The obtained residue was purified by column chromatography (dichloromethane/methanol, 20:1) to obtain a target compound (16 mg, 59% yield) as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 9.91 (brs, 1H), 8.62 (s, 1H), 7.49 (d, J=7.6 Hz, 1H), 7.28H (m, 2H), 7.15 (t, J=7.6 Hz, 2H), 7.02 (t, J=8.0 Hz, 1H), 7.00 (d, J=8.8 Hz, 2H), 6.51 (s, 1H), 3.93 (s, 6H), 3.26 (t, J=4.6 Hz, 4H), 2.64 (t, J=4.4 Hz, 4H), 2.36 (q, J=7.2 Hz, 2H), 1.15 (t, J=7.2 Hz, 3H). 13C NMR (400 MHz, CDCl3) δ 163.49, 158.07, 156.36, 154.72, 150.63, 149.39, 142.67, 135.44, 131.39, 129.12, 124.81, 123.28, 120.86, 117.78, 116.76, 112.85, 110.17, 95.25, 92.88, 56.47, 52.64, 52.30, 49.03, 11.85. MS m/z: 620 [M+1]+.
Example 2
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((2-methoxy-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 2-methoxy-4-(4-methylpiperazin-1-yl)aniline. 1H NMR (400 MHz, CDCl3) δ 9.87 (brs, 1H), 8.65 (d, J=0.4 Hz, 1H), 7.56 (brs, 1H), 7.50 (d, J=6.8 Hz, 1H), 7.37 (d, J=8.0 Hz, 1H), 7.16 (t, J=7.6 Hz, 1H), 7.09-7.04 (m, 2H), 6.88 (brs, 1H), 6.57 (m, 2H), 6.52 (s, 1H), 3.93 (s, 6H), 3.89 (s, 3H), 3.29 (t, J=4.4 Hz, 4H), 2.70 (brs, 4H), 2.44 (s, 3H). 13C NMR (400 MHz, CDCl3) δ 163.14, 158.15, 156.50, 154.78, 150.39, 142.97, 135.57, 131.54, 124.66, 123.26, 120.76, 118.84, 118.21, 112.80, 110.05, 108.22, 100.49, 95.31, 93.32, 56.56, 55.64, 54.78, 49.03, 45.71. MS m/z: 635 [M+1]+.
Example 3
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-morpholinophenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 4-morpholinoaniline 1H NMR (400 MHz, CDCl3) 9.95 (brs, 1H), 8.62 (d, J=0.4 Hz, 1H), 7.47 (brs, 2H), 7.32 (d, J=8.4 Hz, 3H), 7.14 (t, J=7.4 Hz, 1H), 7.03 (t, J=7.6 Hz, 1H), 6.97 (d, J=8.8 Hz, 2H), 6.91 (brs, 1H), 6.49 (s, 1H), 3.91 (s, 6H), 3.89 (t, J=4.8 Hz, 4H), 3.18 (t, J=4.4 Hz, 4H). 13C NMR (400 MHz, CDCl3) 163.51, 158.18, 456.49, 154.82, 149.59, 135.32, 131.21, 129.24, 128.78, 125.21, 123.44, 121.06, 117.94, 116.58, 112.77, 110.21, 66.78, 56.55, 49.34. MS m/z: 593 [M+1]+.
Example 4
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((2-methoxy-4-morpholinophenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 2-methoxy-4-morpholinoaniline 1H NMR (400 MHz, CDCl3) 9.90 (brs, 1H), 8.65 (d, J=0.8 Hz, 1H), 7.58 (brs, 1H), 7.52 (brs, 1H), 7.38 (brs, 1H), 7.17 (t, J=7.4 Hz, 1H), 7.12 (brs, 1H), 7.09-0.05 (m, 1H), 6.88 (brs, 1H), 6.56 (m, 2H), 6.52 (s, 1H), 3.93 (s, 6H), 3.89 (t, J=4.8 Hz, 3H), 3.89 (s, 3H), 3.19 (t, J=4.8 Hz, 4H). MS m/z: 622 [M+1]+.
Example 5
1-(6-((4-([1,4′-bipiperidin]-1′-yl)-3-(trifluoromethyl)phenyl)amino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 4-([1,4′-bipiperidin]-1′-yl)-3-(trifluoromethyl)aniline 1H NMR (400 MHz, CDCl3) δ 11.65 (s, 1H), 9.90 (brs, 1H), 8.69 (s, 1H), 8.05 (s, 1H), 7.77 (d, J=7.6 Hz, 1H), 7.65 (brs, 1H), 7.48 (brs, 1H), 7.41 (brs, 1H), 7.22 (d, J=8.8 Hz, 1H), 7.15 (t, J=7.4 Hz, 1H), 7.08 (t, J=7.8 Hz, 1H), 6.47 (s, 1H), 3.89 (s, 6H), 3.26 (d, J=10.8 Hz, 2H), 3.13 (t, J=10.0 Hz, 1H), 3.00 (d, J=10.4 Hz, 2H), 2.76 (q, J=11.07 Hz, 2H), 2.66 (t, J=11.0 Hz, 2H), 2.37 (q, J=12.53 Hz, 2H), 2.25 (d, J=10.0 Hz, 2H), 1.87 (m, 5H), 1.35 (q, J=12.8 Hz, 1H). MS m/z: 741 [M+1]+.
Example 6
1-(6-((4-((4-(cyclohexylmethyl)piperazin-1-yl)methyl)phenyl)amino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine




1-(6-Chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 4-((4-(cyclohexylmethyl)piperazin-1-yl)methyl)aniline were dissolved in a mixture of 1,4-dioxane and dimethyl sulfoxide. After adding tBu-XPhos and potassium carbonate, gas was removed for 5 minutes using an ultrasound sonicator. Then, after adding Pd2(dba)3, the mixture was stirred at 120° C. for 20 hours. After the reaction was completed, the mixture was cooled to room temperature and the solid residue was filtered. After extracting several times using ethyl acetate, the obtained organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The obtained residue was purified by column chromatography to obtain a target compound. MS m/z: 702 [M+1]+.
Example 7
methyl 4-((6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-1-yl)amino)-3-methoxybenzoate




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and methyl 4-amino-3-methoxybenzoate. 1H NMR (400 MHz, CDCl3) δ 9.94 (brs, 1H), 8.80 (s, 1H), 8.37 (d, J=8.4 Hz 1H), 7.76 (d, J=8.4 Hz, 1H), 7.63 (s, 1H), 7.59 (brs, 2H), 7.22 (t, J=7.6 Hz, 1H), 7.16 (t, J=7.6 Hz, 1H), 6.51 (s, 1H), 4.01 (s, 3H), 3.93 (s, 3H), 3.93 (s, 6H). MS m/z: 595 [M+1]+.
Example 8
1-(6-aminopyrimidin-1-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine




1-(6-Chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and ammonium chloride were dissolved in a mixture of 1,4-dioxane and dimethyl sulfoxide. After adding diisopropylethylamine, the mixture was stirred at 120° C. for 20 hours. After the reaction was completed, the mixture was cooled to room temperature and extracted several times using ethyl acetate. The obtained organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The obtained residue was purified by column chromatography to obtain a target compound. 1H NMR (400 MHz, CDCl3) δ 9.80 (brs, 1H), 8.58 (d, J=0.8 1H), 7.53 (brs, 1H), 7.47 (brs, 1H), 7.17 (t, J=7.2 Hz, 1H), 7.10 (m, 1H), 6.89 (brs, 1H), 6.51 (s, 1H), 5.42 (s, 2H), 3.93 (s, 6H). MS m/z: 431 [M+1]+.
Example 9
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(methylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as Example 8 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and methylamine hydrochloride. 1NMR (400 MHz, CDCl3) δ 9.99 (brs, 1H), 8.56 (s, 1H), 7.52 (brs, 1H), 7.49 (brs, 1H), 7.17 (t, J=7.4 Hz, 1H), 7.09 (t, J=7.4 Hz, 1H), 6.70 (brs, 1H), 6.49 (s, 1H), 5.73 (brs, 1H), 3.91 (s, 6H), 3.03 (d, J=5.2 Hz, 3H). MS m/z: 445 [M+1]+.
Example 10
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(phenylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and aniline 1H NMR (400 MHz, CDCl3) δ 9.94 (brs, 1H), 8.67 (d, J=0.4 Hz, 1H), 7.76 (brs, 1H), 7.44 (m, 5H), 7.33 (brs, 1H), 7.23 (brs, 1H), 7.13 (t, J=7.6 Hz, 1H), 7.00 (brs, 1H), 6.98 (t, J=7.8 Hz, 1H), 6.45 (s, 1H), 3.88 (s, 6H). MS m/z: 508 [M+1]+.
Example 11
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-(pyrimidin-2-ylamino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




1-(6-Chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 2-aminopyridine were dissolved in a mixture of 1,4-dioxane and dimethyl sulfoxide. After adding BINAP and potassium carbonate, gas was removed for 5 minutes using an ultrasound sonicator. Then, after adding Pd(OAc)2, the mixture was stirred at 120° C. for 20 hours. After the reaction was completed, the mixture was cooled to room temperature and the solid residue was filtered. After extracting several times using ethyl acetate, the obtained organic layer was washed with water and a saturated sodium chloride solution, dried using sodium sulfate and then concentrated. The obtained residue was purified by column chromatography to obtain a target compound. MS m/z: 509 [M+1]+.
Example 12
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((6-(4-ethylpiperazin-1-yl)pyrimidin-4-yl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 6-(4-ethylpiperazin-1-yl)pyrimidin-4-amine. MS m/z: 622 [M+1]+.
Example 13
N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((1-methylpiperidin-4-yl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and 1-methylpiperidin-4-amine. MS m/z: 528 [M+1]+.
Example 14
N1-(6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-4-yl)-N2,N2-dimethylethane-1,2-diamine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and N,N-dimethylethane-1,2-diamine. MS m/z: 502 [M+1]+.
Example 15
N1-(6-(2-((2,6-dichloro-3,5-dimethoxyphenyl)amino)-1H-benz[d]imidazol-1-yl)pyrimidin-4-yl)-N3,N3-dimethylpropane-1,3-diamine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and N,N-dimethylpropane-1,3-diamine. MS m/z: 516 [M+1]+.
Example 16
1-(6-(cyclopropylamino)pyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and cyclopropylamine. MS m/z: 471 [M+1]+.
Example 17
N-(3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(3,5-dimethoxyphenyl)-1H-benz[d]imidazol-2-amine and (4-ethylpiperazin-1-yl)phenylamine. MS m/z: 551.67 [M+1]+.
Example 18
N-(3,5-dimethoxyphenyl)-3-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 3-(6-chloropyrimidin-4-yl)-N-(3,5-dimethoxyphenyl)-3H-imidazo[4,5-c]pyridin-2-amine and (4-ethylpiperazin-1-yl)phenylamine. MS m/z: 552.66 [M+1]+.
Example 19
N-(3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-amine




A target compound was obtained in the same manner as the step 7 of Example 1 using 1-(6-chloropyrimidin-4-yl)-N-(3,5-dimethoxyphenyl)-1H-imidazo[4,5-b]pyrazin-2-amine and (4-ethylpiperazin-1-yl)phenylamine. MS m/z: 553.64 [M+1]+.
Example 20
N-(3-((3-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide




A target compound was obtained in the same manner as the step 7 of Example 1 using N-(3-((3-(6-chloropyridin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide and (4-ethylpiperazin-1-yl)phenylamine. MS m/z: 693.75 [M+1]+.
Example 21
N-(3-((1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide




A target compound was obtained in the same manner as the step 7 of Example 1 using N-(3-((1-(6-chloropyridin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide and (4-ethylpiperazin-1-yl)phenylamine. MS m/z: 694.74 [M+1]+.
Example 22
4-methyl-N-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenyl)-3-((3-(6-(phenylamino)pyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)benzamide




A target compound was obtained in the same manner as the step 7 of Example 1 using 3-((3-(6-chloropyrimidin-4-yl)-3H-imidazo[4,5-c]pyridin-2-yl)amino)-4-methyl-N-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenyl)benzamide and aniline MS m/z: 661.67 [M+1]+.
The novel compound represented by Chemical Formula 1 according to the present invention can be prepared into various formulations depending on purposes. Some formulation examples containing the compound represented by Chemical Formula 1 according to the present invention as an active ingredient are described below. However, the present invention is not limited by them.
FORMULATION EXAMPLES
Formulation Example 1
Tablet (Direct Compression)
5.0 mg of the active ingredient was sieved, mixed with 14.1 mg of lactose, 0.8 mg of crospovidone USNF and 0.1 mg of magnesium stearate and then compressed into a tablet.
Formulation Example 2
Tablet (Wet Granulation)
5.0 mg of the active ingredient was sieved and mixed with 16.0 mg of lactose and 4.0 mg of starch. After adding a solution of 0.3 mg of polysorbate 80 in pure water, the mixture was prepared into a granule. After drying, the granule was sieved and mixed with 2.7 mg of colloidal silicon dioxide and 2.0 mg of magnesium stearate. The granule was compressed into a tablet.
Formulation Example 3
Powder and Capsule
5.0 mg of the active ingredient was sieved and mixed with 14.8 mg of lactose, 10.0 mg of polyvinylpyrrolidone and 0.2 mg of magnesium stearate. The mixture was filled in a No. 5 gelatin capsule using an adequate apparatus.
Formulation Example 4
Injection
An injection was prepared using 100 mg of the active ingredient, 180 mg of mannitol, 26 mg of Na2HPO4.12H2O and 2974 mg of distilled water.
Test Examples
Test Example 1
Measurement of Kinase Inhibition Activity
In order to measure the inhibition activity (% inhibition) of the compound of the present invention against protein kinases, a biochemical assay was conducted using a full kinase panel consisting of 340 kinases. As a representative compound of the present invention, N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1 was used.
The kinases whose activity is inhibited by 50% or more when treated with 1 μM of the compound of Example 1 are listed in Table 1.







TABLE 1





Kinases whose activity is inhibited by 50% or more


















ABL2
ACK1



ALK
BLK



BMX
c-Kit



c-Src
DDR1



DDR2
DYRK1



DYRK1B
DYRK2



EGFR
HER2



FGFR1
FGFR2



FGFR3
FGFR4



FGF
FLT3



FLT4
FMS



Fyn
Hck



MAP4K5
LCK



LIMK1
LYN



LYN B
MEKK2



MEKK3
ROS



SIK1
SIK2



SIK3
TAOK1



TAOK2
TIE2



TNK1
TRKA



YES










Test Example 2
Evaluation of Ability of Inhibiting Proliferation of Cancer Cells
The ability of the compound of the present invention of inhibiting the proliferation of the bladder cancer cell line RT112 was determined as the GI50 value. The result is shown in Table 2.








TABLE 2






Ability of


Compounds
inhibiting proliferation of RT112 (GI50, μM)
















Compound of Example 1
<1


Compound of Example 2
<1


Compound of Example 3
<1


Compound of Example 4
<1


Compound of Example 5
<10


Compound of Example 6
<10


Compound of Example 8
<1


Compound of Example 9
<1


Compound of Example 10
<1


Compound of Example 11
<1









Test Example 3
Evaluation of Inhibition Activity Against Pan-FGFR Mutants
The inhibition activity of N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1, as a representative compound of the present invention, against pan-FGFR mutants was measured. Enzymatic activity and cellular activity were measured. The result is shown in Table 3 and Table 4.










TABLE 3






FGFR1 mutant
FGFR2 mutant
FGFR3 mutant


Mutant enzymes
(V561M)
(N549H)
(K650E)







Compound of
10 nM
901 nM
4.8 nM


Example 1





















TABLE 4






AN3-






Mutant cells
CA
MFE296
SNU-16
RT-112
OPM-2







Compound of Example
53 nM
1600 nM
2.7 nM
29 nM
43 nM


1









Test Example 4
Evaluation of Inhibition Activity Against CYPs (Cytochromes P450)
The inhibition activity (inhibition rate, %) of N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1, as a representative compound of the present invention, against CYPs (cytochromes P450) was measured in order to investigate the risk of drug side effects. The result is shown in Table 5. It can be seen that the compound of the present invention exhibits no inhibition activity against the five CYPs: CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4.










TABLE 5









Concentration
CYPs














(μM)
CYP1A2
CYP2C9
CYP2C19
CYP2D6
CYP3A4

















%
0
0.00
0.00
0.00
0.00
0.00


inhibition
0.1
5.36
10.26
2.72
−3.15
3.09



0.5
4.67
8.79
3.23
−4.76
3.70



5
−1.90
24.59
30.01
−2.56
24.44



10
−3.46
29.97
37.26
6.12
31.82



25
−3.11
52.41
66.07
13.61
49.66



50
2.08
53.37
67.96
13.38
55.49



Positive control
52.04
95.56
89.08
97.07
88.98












Expected IC50
>50
44.82
16.64
>50
32.35









Test Example 5
Evaluation of Microsomal Stability
The microsomal stability of N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1, as a representative compound of the present invention, was evaluated. The result is shown in Table 6.











TABLE 6









Negative






control


Tested

Compound of
Positive control
(excluding


species
Time (min)
Example 1
(midazolam)
NADPH)



















Mouse
0
100.00%
100.00%
100.00%



5
62.73%
10.42%
103.56%



10
40.77%
1.13%
103.56%



15
29.27%
0.18%
102.67%



30
11.88%
0.05%
106.67%



45
3.87%
—
106.67%



60
3.64%
—
104.44%



T1/2 (min)
9.93
NA
NA


Rat
0
100.00%
100.00%
—



5
95.53%
13.65%
—



10
88.45%
1.01%
—



15
82.61%
0.14%
—



30
65.16%
0.04%
—



45
45.27%
—
—



60
44.31%
—
—



T1/2 (min)
39.56
NA
NA


Dog
0
100.00%
100.00%
—



5
77.02%
6.23%
—



10
57.41%
0.59%
—



15
44.70%
0.27%
—



30
21.03%
0.10%
—



45
8.23%
—
—



60
5.02%
—
—



T1/2 (min)
13.50
NA
NA


Human
0
100.00%
100.00%
—



5
80.36%
38.75%
—



10
76.63%
12.92%
—



15
64.04%
5.60%
—



30
51.45%
0.73%
—



45
37.62%
—
—



60
36.17%
—
—



T1/2 (min)
33.94
NA
NA









Test Example 6
In-Vivo PK Profile
The in-vivo PK profile (mouse PK/ICR mice and rat PK/Sprague-Dawley rat) of N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1 is as follows.







TABLE 7







Mouse PK/ICR mice

















t1/2
Tmax
Cmax
AUC(0-t)
AUC(0-∞)
Vz
CL
MRT(0-∞)
F*


Dosage
hr
hr
ng/mL
ng/mL*hr
ng/mL*hr
L/kg
L/hr/kg
hr
%



















IV 5 mg/kg
1.83
0.083
111.03
321.99
350.27
37.69
14.27
3.56
—


PO 10 mg/kg
NA
6.00
27.07
150.93
NA
—
—
NA
23.44
















TABLE 8







Rat PK/Sprague-Dawley rat

















t1/2
Tmax
Cmax
AUC(0-t)
AUC(0-∞)
Vz
CL
MRT(0-∞)
F*


Dosage
hr
hr
ng/mL
ng/mL*hr
ng/mL*hr
L/kg
L/hr/kg
hr
%



















IV 5 mg/kg
5.75
0.083
270.95
844.42
884.29
47.99
5.84
6.37
—


PO 5 mg/kg
9.14
5.33
11.59
129.20
652.26
—
—
12.57
13.46









Test Example 7
Evaluation of In-Vivo Efficacy in Endometrial Cancer Cell Line-Transplanted Animal Model
The in-vivo efficacy of N-(2,6-dichloro-3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-benz[d]imidazol-2-amine synthesized in Example 1 was evaluated as follows.
Four-to-five-week old BALB/C nude mouse transplanted with the FGFR2-overexpressed endometrial cancer cell line AN3-CA was used as an animal model. AZD4547 (AstraZeneca) known as an FGFR inhibitor was used as a control drug. A mixture solution (pH=4) of N-methylpyrrolidinone (NMP, 5%), Solutol (6%), polyethylene glycol (PEG 400, 20%) and distilled water (69%) was used as a vehicle. A result obtained by orally administering the drug once a day is shown in FIG. 1.
As described above, since the heteroarylamine compound represented by Chemical Formula 1 according to the present invention or a pharmaceutically acceptable salt thereof exhibits inhibition activity against protein kinases, it is useful for prevention and treatment of diseases caused by abnormal cell growth induced by protein kinases, for example, various tumor diseases selected from endometrial cancer, bladder cancer, stomach cancer, lung cancer, liver cancer, colon cancer, small intestine cancer, pancreatic cancer, brain cancer, bone cancer, melanoma, breast cancer, sclerosing adenosis, head and neck cancer, esophageal cancer, thyroid cancer, parathyroid cancer, kidney cancer, sarcoma, prostate cancer, urethral cancer, blood cancer such as leukemia, multiple myeloma and myelodysplastic syndrome, lymphoma such as Hodgkin's disease and non-Hodgkin lymphoma, fibroadenoma, etc.

## Claims

1. A compound selected from a heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a hydrate thereof and a solvate thereof:



wherein,
R1 is one to four substituent(s) selected from a halogen atom, C1-C8 alkyl, C1-C8 alkoxy and




R2 is a hydrogen atom, C1-C8 alkyl, —(CH2)m—NR3R4 or -A-(CH2)n-(Q)p-R5,
A is C6-C15 aryl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) or 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein each of the aryl, heteroaryl or heterocycloalkyl may be substituted or unsubstituted with one to three substituent(s) selected from C1-C8 alkyl, C1-C8 alkoxy, C1-C8 haloalkyl and C1-C8 alkoxycarbonyl,
L is —NHC(O)— or —C(O)NH—,
Z is one to four substituent(s) selected from C1-C8 alkyl, C1-C8 haloalkyl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) and 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein the heteroaryl or heterocycloalkyl may be substituted or unsubstituted with C1-C8 alkyl,
Q is 5- or 6-membered heterocycloalkyl or biheterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom,
each of R3 and R4 is a hydrogen atom or C1-C8 alkyl,
R5 is a hydrogen atom, C(O)OR7 (wherein R7 is a hydrogen atom or C1-C8 alkyl) or C1-C8 alkyl,
m is an integer from 1 to 6,
n is an integer from 0 to 6,
p, which indicates the presence or absence of Q, is 0 or 1, and
wherein X1 and X4 are N and X2 and X3 are CH.

2. The compound according to claim 1, wherein R1 is one to four substituent(s) selected from chloro, methyl, methoxy, —NHC(O)-(3-trifluoromethylbenzene) and —C(O)NH-(3-trifluoromethylbenzene).

3. The compound according to claim 1, wherein R2 is a hydrogen atom.

4. The compound according to claim 1, wherein R2 is methyl, ethyl or cyclopropyl.

5. The compound according to claim 1, wherein R2 is 2-(dimethylamino)ethyl or 3-(dimethylamino)propyl.

6. The compound according to claim 1, wherein R2 is phenyl, methoxy-substituted phenyl, trifluoromethyl-substituted phenyl, methoxy- and methoxycarbonyl-substituted phenyl, pyridinyl, pyrimidinyl, 4-methylpiperidin-1-yl or 4-ethylpiperidin-1-yl.

7. The compound according to claim 1, wherein R2 is



and wherein each of X and Y, which are identical to or different from each other, is CH or N, Q is piperidinyl, bipiperidinyl, piperazinyl or morpholino, R5 is a hydrogen atom, methyl, ethyl, cyclohexylmethyl or methoxycarbonyl, R6 is a hydrogen atom, methoxy, trifluoromethyl or methoxycarbonyl and n is an integer from 0 to 3.

8. A compound selected from a heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a hydrate thereof and a solvate thereof;



wherein,
R1 is one to four substituent(s) selected from a halogen atom, C1-C8 alkyl, C1-C8 alkoxy and




R2 is a hydrogen atom, C1-C8 alkyl, —(CH2)m—NR3R4 or -A-(CH2)n-(Q)p-R5,
A is C6-C15 aryl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) or 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein each of the aryl, heteroaryl or heterocycloalkyl may be substituted or unsubstituted with one to three substituent(s) selected from C1-C8 alkyl, C1-C8 alkoxy, C1-C8 haloalkyl and C1-C8 alkoxycarbonyl,
L is —NHC(O)— or —C(O)NH—,
Z is one to four substituent(s) selected from C1-C8 alkyl, C1-C8 haloalkyl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) and 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein the heteroaryl or heterocycloalkyl may be substituted or unsubstituted with C1-C8 alkyl,
Q is 5- or 6-membered heterocycloalkyl or biheterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom,
each of R3 and R4 is a hydrogen atom or C1-C8 alkyl,
R5 is a hydrogen atom, C(O)OR7 (wherein R7 is a hydrogen atom or C1-C8 alkyl) or C1-C8 alkyl,
m is an integer from 1 to 6,
n is an integer from 0 to 6, and
p, which indicates the presence or absence of Q, is 0 or 1,
wherein X1 and X4 are N and X2 and X3 are CH, and
wherein the compound of Chemical Formula 1 is
N-(3,5-dimethoxyphenyl)-1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-amine or
N-(3-((1-(6-((4-(4-ethylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazin-2-yl)amino)-4-methylphenyl)-3-(trifluoromethyl)benzamide.

9. A method of treating a tumor disease selected from the group consisting of endometrial cancer, bladder cancer, stomach cancer, lung cancer, liver cancer, colon cancer, small intestine cancer, pancreatic cancer, brain cancer, bone cancer, melanoma, breast cancer, sclerosing adenosis, head and neck cancer, esophageal cancer, thyroid cancer, parathyroid cancer, kidney cancer, sarcoma, prostate cancer, urethral cancer, leukemia, multiple myeloma, blood cancer, lymphoma and fibroadenoma, in a patient comprising administering to the patient a compound according to claim 1.

10. A method of inhibiting the proliferation of a cell line selected from the group consisting of AN3-CA, MFE296, SNU-16, RT112 or OPM-2 comprising contacting the cell line with a compound according to claim 1.

11. A compound selected from a heteroarylamine compound represented by Chemical Formula 1, a pharmaceutically acceptable salt thereof, a hydrate thereof and a solvate thereof:



wherein
R1 is one to four substituent(s) selected from a halogen atom, C1-C8 alkyl, C1-C8 alkoxy and




R2 is a hydrogen atom, C1-C8 alkyl, —(CH2)m—NR3R4 or -A-(CH2)n-(Q)p-R5,
A is C6-C15 aryl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) or 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein each of the aryl, heteroaryl or heterocycloalkyl may be substituted or unsubstituted with one to three substituent(s) selected from C1-C8 alkyl, C1-C8 alkoxy, C1-C8 haloalkyl and C1-C8 alkoxycarbonyl,
L is —NHC(O)— or —C(O)NH—,
Z is one to four substituent(s) selected from C1-C8 alkyl, C1-C8 haloalkyl, 5- or 6-membered heteroaryl containing one to three nitrogen atom(s) and 5- or 6-membered heterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom, wherein the heteroaryl or heterocycloalkyl may be substituted or unsubstituted with C1-C8 alkyl,
Q is 5- or 6-membered heterocycloalkyl or biheterocycloalkyl containing one to three heteroatom(s) selected from a nitrogen atom and an oxygen atom,
each of R3 and R4 is a hydrogen atom or C1-C8 alkyl,
R5 is a hydrogen atom, C(O)OR7 (wherein R7 is a hydrogen atom or C1-C8 alkyl) or C1-C8 alkyl,
m is an integer from 1 to 6,
n is an integer from 0 to 6,
p, which indicates the presence or absence of Q, is 0 or 1, and
wherein X1, X3 and X4 are CH and X2 is N.