## Page 1 of 3 For Experimental Use Only

For use only at an application site of a cooperator or participant and in accordance with the terms and conditions of the Experimental Use Permit. Not for sale to any person other than a participant or cooperator of the EPA-approved Experimental Use Permit program. This label must be in possession of the user at the time of pesticide application. For use only in the state of California.

Read safety directions before using. 

# Wolbachia pipientis, w AlbB Strain

in male Aedes aegypti WB1 strain mosquitoes EPA Experimental Use Permit No. 88877-EUP-2

ACTIVE INGREDIENT: Wolbachia pipientis, w AlbB Strain*:...........................100.00% OTHER INGREDIENTS:........................................................0.00% Total:.................................................................................100.00%

* T he Active Ingredient is Wolbachia pipientis

, w

AlbB Strain, intracellular, obligate, and naturally-occurring bacteria in male Aedes aegypti WB1 strain mosquitoes.



## KEEP OUT OF REACH OF CHILDREN



## CAUTION

Net contents: 0.236 oz. (6.7 g) maximum adult male Aedes aegypti mosquitoes, with each mosquito containing approximately 0.001% Wolbachia pipientis , w AlbB Strain.

EPA Experimental Use Permit No.: 88877-EUP-2. Lot # _______

Expiration of male mosquito batch date: 24 hours after receipt of shipping container, except i f male mosquitoes are put in cages and fed sucrose upon receipt of the shipping container, then they may be kept for up to 3 days prior to release.

EPA Est. Nos.: 88877-KY-001 and/or 89668-KY-001

University of Kentucky, Department of Entomology S-225 Agricultural Science Center, North Lexington, KY 40546-0091 (859) 257-4902 Office (859) 257-5117 Lab (859) 323-1120 Fax e-mail: sdobson@email.uky.edu 

### 88877-EUP-2 10/15/2015

## Page 2 of 3 PRECAUTIONARY STATEMENTS ENVIRONMENTAL HAZARDS Do not contaminate water when disposing of equipment washwater or rinsate.



### DIRECTIONS FOR USE FOR EXPERIMENTAL USE ONLY It is a violation of Federal law to use this product in a manner inconsistent with its labeling. For experimental use by The University of Kentucky, Department of Entomology; the Consolidated Mosquito Abatement District of Selma; and their cooperators in California under the EPA Experimental Use Permit (EUP) for control of Aedes aegypti m osquitoes. Only male (and no female) Ae. aegypti strain WB1 mosquitoes carrying Wolbachia pipientis, w AlbB Strain microbial pesticide are to be released. The mosquitoes and their life stages are for use only by the staff, technicians, and/or cooperators of The University of Kentucky, Department of Entomology and the Consolidated Mosquito Abatement District of Selma specifically trained for this purpose. Before making the first applications under this EUP, consult with the state agency with primary responsibility for pesticide regulation to determine if other regulatory requirements exist. Releases of male mosquitoes are done on a weekly basis with three releases per week, totaling ~100,000 adult male mosquitoes containing W. pipientis

, w AlbB Strain. Male mosquitoes are released to the air and fly away to mate in the air. All of the use sites for this EUP are included in Section G of the application (on Field Trial Information Requirements Forms). All mosquito rearing is done at EPA Est. Nos. 88877-KY-001 and/or 89668-KY-001.

100,000 Ae. aegypti males that carry W. pipientis

, w AlbB Strain microbial pesticide are to be released per week for up to six months in 2015 and 2016 at the above identified sites

. To measure for population control effects, mosquito populations will be sampled using mosquito traps to monitor adults and eggs. These mosquitoes typically lay eggs in containers, such as discarded cups or bottles.

Mosquitoes are reared in water from a municipal source in plastic pans at ~82.4F (~28°C). Mosquito larvae eat various foods including powdered liver, cat or dog food, or fish food. Immature mosquitoes are kept at a density of approximately one larva/ml. The mosquitoes become pupae in about five days and develop in the pupal stage for two days before separation of the smaller male pupae from larger female pupae by a mechanical separator and by visual sorting. This device separates the smaller male pupae from larger female pupae by the difference in size. The female pupae are killed by freezing and, when dead, put in a Ziploc® -type PE bag and disposed of with trash and/or garbage.

Male mosquitoes are allowed to eclose or emerge as adult males and groups of adult males are visually examined to remove any residual adult females. The visual sorting is repeated a second time prior to shipping, for a total of two visual sorting examinations. Male mosquitoes for release are immobilized by chilling ((59 Û F aÛ& ) for delivery to the release site. They are placed in multiple small plastic containers in a sealable PE bag and then put into an insulated shipping container with common reusable ice cooler packs.

At the release site, adult male mosquitoes are placed on a platform and exposed to ambient natural conditions. After acclimation, they become active and fly away to disperse and mate with indigenous Ae. aegypti female mosquitoes.

## Page 3 of 3 STORAGE AND DISPOSAL Do not contaminate water, food orfeed by storage or disposal. PESTICIDE STORAGE Keep male mosquitoes chilled with ice packs at ~59 Û F (~ Û& ) during delivery to the release site. If male mosquitoes are put in cages and fed sucrose upon receipt of the shipping container at the release site, then they may be kept for up to 3 days prior to release. 

### PESTICIDE DISPOSAL Unwanted female Ae. aegypti mosquitoes are killed by freezing, then put in a Ziploc® -type PE bag and disposed of with trash and/or garbage. At the conclusion of the experimental field trial program, all mosquito colony life stages maintained for the EPA-approved Experimental Program are to be frozen to kill them, then put in a Ziploc ® -type PE bag and disposed of with trash and/or garbage. All equipment used in the rearing process (e.g., cages and larval pans) are to be washed with a commercial disinfectant cleaner. 

### CONTAINER HANDLING Any durable containers that can be reused are to be washed with a commercial disinfectant cleaner. Disposable containers, including plastic containers and bags, are to be rinsed with a commercial disinfectant cleaner to make sure no viable mosquito life stages are present and recycled or disposed of in trash.