p. 1

Table 2 Blank test of the test substance in fis h

W1 W2 A B C D

peak sample fish peak area conc. conc.

weight count in final in fish

No.l No.2 solution

No . No. g g sample std. mg/L μg/g

A 1 8.82 7.65 <912 18241 <0.00025 <0.16

2 7.20 8.51 <912 18241 <0.00025 <0.16

3 11.74 15 .19 <1006 20116 <0.00025 <0.10

4 13.74 13 .53 <1006 20116 <0.00025 <0.1 0

B 1 8.82 7.65 <922 18448 <0.00025 <0.16

2 7.20 8.51 <922 18448 <0.00025 <0.16

3 11.74 15.19 <1020 20402 <0.00025 <0.10

4 13.74 13.53 <1020 20402 <0 .00025 <0 .1 0

~ C 1 8.82 7.65 <1089 21784 <0.00025 <0.16

2 7.20 8.51 <1089 21784 <0.00025 <0.16

3 11.74 15 .19 <1201 24013 <0.00025 <0.10

4 13.74 13 .53 <1201 24013 <0.00025 <0.1 0

D 1 8.82 7.65 <1235 24694 <0.00025 <0.16

2 7.20 8.51 <1235 24694 <0.00025 <0.16

3 11.74 15.19 <1376 27516 <0.00025 <0 .10

4 13.74 13.53 <1376 27516 <0.00025 <0.10

Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Equations : C=A=BXCstd

~ D=A=B xCstdx(FV/1000)x SF=(W 1+W2)x 1000

-a& ~o~ ~
p. 2

Table 3 Recovery test of the test substance in fish

A B C D E R

peak sample fish test peak area conc. measured recovery

weight substance count in final amount

No.l No.2 added solution Average Standard Range

No. No. g g mg sample std. mg/L mg % n=4 deviation

A 1 13.98 4.14 0.05 15171 18232 0.00416 0.0416 83 .2 84.6 3.1 6.9

2 12.30 5.73 0.05 16018 18232 0.00439 0.0439 87. 9

3 12.51 5.98 0.05 14766 18232 0.00405 0.0405 81.0

4 12.33 5.91 0.05 15718 18232 0.00431 0.0431 86.2

B 1 13.98 .4.14 0.05 15340 18549 0.00413 0.0413 82.7 84.6 3.2 7.0

2 12.30 5.73 0.05 16335 18549 0.00440 0.0440 88. 1

3 12.51 5.98 0.05 15044 18549 0.00406 0.0406 81 .1

4 12.33 5.91 0.05 16031 18549 0.00432 0.0432 86. 4

C 1 13.98 4.14 0.05 18824 21914 0.00429 0.0429 85.9 86.3 2.3 5.6

2 12.30 5.73 0.05 19524 21914 0.00445 0.0445 89. 1

3 12.51 5.98 0.05 18304 21914 0.00418 0.0418 83.5

4 12.33 5.91 0.05 18969 21914 0.00433 0.0433 86. 6

D 1 13.98 4.14 0.05 21029 24731 0.00425 0.0425 85.0 86.3 3.9 9.2

2 12.30 5.73 0.05 22607 24731 0.00457 0.0457 91 . 4

3 12.51 5.98 0.05 20333 24731 0.00411 0.0411 82.2

4 12.33 5.91 0.05 21373 24731 0.00432 0.0432 86.4

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Equations : D=B=CxCstd

E= B=CXCstdx.(FV/1000)x SF

t R=B=CxCstdx(FV/1000)xSF=Ax 100
p. 3

Table 4-1 Concentration of the test substance in the test water - High concentration level (Pea k A)

i A B C D

sampling period number peak area conc . mean conc.

date of count in water in water

_ day analysis sample std. mg/L mg/L

2006/05/11 0 1 18559 18464 0.0503 0.0503

2006/05/18 7 2 17072 18738 0.0456 0.0479

2006/05/25 14 3 74769 75391 0.0496 0.0485

2006/06/01 21 4 85849 79289 0.0541 0.0499

2006/06/08 28 5 41124 44333 0.0464 0.0492

2006/06/15 35 6 38036 36313 0.0524 0.0497

2006/06/22 42 7 25519 24612 0.0518 0.0500

2006/06/29 49 8 31049 34034 0.0456 0.0495

2006/07/10 60 9 29606 31130 0.0476 0.0493

Standard deviation : 0.0032 mg/L

Coefficient of variation : 6.5 %

Concentration in water at steady state (28-60day) : 0.0488 mg/L

Concentration of std. solution (Cstd) : 0.005 mg/L

Dillution Ratio(DR): 10

Equations : C=A=BxCstdxDR

D=E{A=BxCstdxDR}i=i

Figure 7-1 Concentration of the test substance in the test water - High concentration level (Peak A)

0.1 0

(~- analyzed value

- - - - - - nominal value

.:,

0.05 .- ------ ------ I

_

0.00

0 7 14 21 28 35 42 49 56

Exposure period (day)

~ VI 3
p. 4

Table 4-2 Concentration of the test substance in the test water - High concentration level (Pe ak B)

_ i A B C D

sampling period number peak area conc. mean conc .

date of count in water in water

day analysis sample std. mg/L mg/L

2006/05/11 0 1 18560 18907 0.0491 0.0491

2006/05/18 7 2 17439 19010 0.0459 0.0475

2006/05/25 14 3 75647 76115 0.0497 0.0482

2006/06/01 21 4 86327 80849 0.0534 0.0495

2006/06/08 28 5 41472 44180 0.0469 0.0490

2006/06/15 35 6 38406 37115 0.0517 0.0495

2006/06/22 42 7 25544 -25176 0.0507 0.0496

2006/06/29 49 8 31847 34465 0.0462 0.0492

2066/07/10 60 9 30538 31254 0.0489 0.0492

Standard deviation : 0.0025 mg/L

Coefficient of variation : 5.1 %

Concentration in water at steady state (28-60day) : 0.0489 mg/L

Concentration of std. solution (Cstd) : 0.005mg/L

Dillution Ratio(DR): 10

Equations : C=A=BxCstdxDR

D=E{A=BXCstdxDR} i=i

Figure 7-2 Concentration of the test substance in the test water - High concentration level (Peak B)

0.1 0

~ • analyzed value

• • • • - . - nominal value

O

0.05 . . ., . ., . . .

W

O

0.00

0 7 14 21 28 35 42 49 56

Exposure period (day)

~ ~~ ~
p. 5

Table 4-3 Concentration of the test substance in the test water - High concentration level (Peak C)

i A B C D

sampling period number peak area conc . mean conc.

date of count in water in water

_ day analysis sample std. mg/L mg/L

2006/05/11 0 1 22666 22400 0.0506 0.0506

2006/05/18 7 2 21194 22125 0.0479 0.0492

2006/05/25 14 3 91144 91116 0.0500 0.0495

2006/06/01 21 4 104128 95473 0 .0545 0.0508

2006/06/08 28 5 52259 53198 0.0491 0.0504

2006/06/15 35 6 46121 44829 0.0514 0.0506

2006/06/22 42 7 31410 30910 0.0508 0.0506

2006/06/29 49 8 38449 40090 0.0480 0.0503

2006/07/10 60 9 35701 36746 0.0486 0.0501

~ Standard deviation : 0.0021 mg/L

Coefficient of variation : 4.2 %

Concentration in water at steady state (28-60day) : 0.0496 mg/L

Concentration of std. solution (Cstd) : 0 .005mg/L

Dillution Ratio(DR): 10

Equations : C=A=BxCstdxDR

D=E{A=BxCstdxDR}i=i

Figure 7-3 Concen tration of the test substance in the test water - High concentration level (Peak C)

0.1 0

( --~- analyzed value

~ nominal valu

~ I

e

o ~ 0.05

~.

~ 0

U

0.00

0 7 14 21 28 35 42 49 56

Exposure period (day)

~f'r lo~ S '
p. 6

Table 4-4 Concentration of the test substance in the test water - High concentration level (Pe ak D)

i A B C D

sampling period number peak area conc. mean conc.

date of count in water in water

_ day analysis sample std. mg/L mg/L

2006/05/11 0 1 25629 24805 0.0517 0.0517

2006/05/18 7 2 23854 24927 0.0478 0.0498

2006/05/25 14 3 103625 102473 0 .0506 0.0500

2006/06/01 21 4 120550 109283 0.0552 0.0513

2006/06/08 28 5 58104 60332 0.0482 0.0507

2006/06/15 35 6 52233 50190 0.0520 0.0509

2006/06/22 42 7 34926 33664 0.0519 0.0510

2006/06/29 49 8 42529 46765 0.0455 0.0503

2006/07/10 60 9 39797 42469 0.0469 0.0500

{ Standard deviation : 0.0031 mg/L

Coefficient of variation : 6.2 %

Concentration in water at steady state (28-60day) : 0.0489 mg/L

Concentration of std . solution (Cstd) : 0.005mg/L

Dillution Ratio(DR): 10

Equations : C=A=BxCstdxDR

D =E {A=B XCstdXDR} i=i

Figure 7-4 Concentration of the test substance in the test water - High concentration level (Peak D)

0.1 0

~ • analyzed value

r

on

. '

. . . . . nominal value

O

t4 0.0 5 . . . . . . . . . . . . . . . . . . . ., . . . . . . . . . , . ., . ., . . . . .

U

r_O

U

0.00

0 7 14 21 28 35 42 49 56

Exposure period (day)

-~- kC
p. 7

Table 5-1 Concentration of the test substance in the test water - Low concentration level (Peak A)

i A B C D

sampling period number peak area conc . mean conc.

date of count in water in water

day analysis sample std mglL mg/L

2006/05/11 0 1 18713 18464 0.00507 0.00507

2006/05/18 7 2 17009 18738 0.00454 0.00480

2006/05/25 14 3 75021 75391 0.00498 0.00486

2006/06/01 21 4 77682 79289 0.00490 0.00487

2006/06/08 28 5 43617 44333 0.00492 0.00488

2006/06/15 35 6 37321 36313 0.00514 0.00492

2006/06/22 42 7 24741 24612 0.00503 0 .00494

2006/06/29 49 8 33553 34034 0 .00493 0.00494

2006/07/10 60 9 32890 31130 0.00528 0.00498

Standard deviation : 0.00020 mg/I,

Coefficient of variation : 4.0 %

Concentration in water at steady state (28-60day) : 0.00506 mg/L

Concentration of std. solution (Cstd) : 0 .005rng/L

Equations : C=A=BxCstd

D=E{ABxCstd}i=i

Figure 8-1 Concentration of the test substance in the test water - Low concentration level (Peak A)

0.010

• analyzed value

au . • . . •• . nominal value

E~

0

c I

0.005

U

U

0.000

0 7 14 21 28 35 42 49 56

Exposure period (day)

-R,8- ~~ ~
p. 8

Table 5-2 Concentration of the test substance in the test water - Low concentration level (Peak B )

_ i A B C D

sampling period number peak area conc . mean conc.

date of count in water in water

day analysis sample std. mg/L mg/L

2006/05/11 0 1 19002 18907 0.00503 0.00503

2006/05/18 7 2 17294 19010 0.00455 0.00479

2006/05/25 14 3 75794 76115 0.00498 0.00485

2006/06/01 21 4 79041 80849 0:00489 0.00486

2006/06/08 28 5 44161 44180 0.00500 0.00489

2006/06/15 35 6 37752 37115 0.00509 0.00492

2006/06/22 42 7 24770 25176 0.00492 0.00492

2006/06/29 49 8 33658 34465 0.00488 0.00492

2006/07/10 60 9 33223 31254 0.00531 0.00496

~ Standard deviation : 0.00020 mg/L

Coefficient of variation : 4.0 %

Concentration in water at steady state (28-60day) : 0.00504 mg/L

Concentration of std . solution (Cstd) : 0.005mg/L

Equations : C=A=BxCstd

D=E{A=BxCstd }i=i

Figure 8-2 Concentration of the test substance in the test water - Low concentration level (Peak B)

0.010

-♦- analyzed value

. . . . . . . nominal value

.'".'

0

0.005 . . . . . .

cd

i .

~

r

N

U

O

U

0.000

0 7 14 21 28 35 42 49 56

Exposure period (day)
p. 9

Table 5-3 Concentration of the test substance in the test water - Low concentration level (Peak C )

i A B C D

sampling period number peak area conc . mean conc.

date of count in water in water

day analysis sample std. mg/L mg/L

2006/05/11 0 1 22689 22400 0.00506 0.00506

2006/05/18 7 2 21014 22125 0.00475 0.00491

2006/05/25 14 3 91219 91116 0.00501 0.00494

2006/06/01 21 4 95653 95473 0.00501 0.00496

2006/06/08 28 5 54099 53198 0.00508 0.00498

2006/06/15 35 6 45702 44829 . 0.00510 0.00500

2006/06/22 42 7 31673 30910 0.00512 0.00502

2006/06/29 49 8 40844 40090 0.00509 0.00503

2006/07/10 60 9 39756 36746 0.00541 0.00507

~ Standard deviation : 0.00017 mg/L

Coefficient of variation : 3.4 %

Concentration in water at steady state (28-60day) : 0.00516 mg/L

Concentration of std. solution (Cstd) : 0.005 mg/L

Equations : C=A=BxCstd

D=E{A=BxCstd }i=i

Figure 8-3 Concentration of the test substance in the test water - Low concentration level (Peak C)

0.010

~- analyzed value

t -a . . . . . . . nominal value 6b

.:. .

~

~ 0.005 ~

~

aa~

U

C

O

0.000

0 7 14 21 28 35 42 49 .56

Exposure period (day)

,4-0- l~-l `~
P. 10

Table 5-4 Concentration of the test substance in the test water - Low concentration level (Peak D)

i A B C D

sampling period number peak area conc. mean conc.

date of count in water in water

day analysis sample std. mg/L mg/L

2006/05/11 0 1 25476 24805 0.00514 0.00514

2006/05/18 7 2 23766 24927 0.00477 0.00495

2006/05/25 14 3 103684 102473 0.00506 0.00499

2006/06/01 21 4 108755 109283 0.00498 0.00498

2006/06/08 28 5 60647 60332 0.00503 0.00499

2006/06/15 35 6 51994 50190 0.00518 0.00502

2006/06/22 42 7 34296 33664 0.00509 0.00503

2006/06/29 49 8 45521 46765 0 .00487 0.00501

2006/07/10 60 9 44931 42469 0 .00529 0.00504

~ Standard deviation : 0.00016 rng/L

Coefficient of variation : 3.2 %

Concentration in water at steady state (28-60day) : 0.00509 mg/L

Concentration of std. solution (Cstd) : 0.005 mg/L

Equations : C=A=BXCstd

D=E{A=BxCstd }i=i

Figure 8-4 Concentration of the test substance in the test water - Low concentration level (Peak D)

0.010

-+- analyzed value

~ -~ .•••••• nominal value

~

c - 0

0 .005

~. ~

~~

U

~

O

U

0.000

0 7 14 21 28 35 42 49 56

Exposure period (day)

..4}- l,o~ c~
P. 11

Table 6-1 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- High concentration level (Peak A)

W1 W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.l No.2 count solution body in water

day No. g g sample std. mg/L μg/g mg/L

7 1 10.98 9 .17 39672 39020 0 .00508 20 59.6 0.0479 1250 1310

7 2 11.57 9 .47 45238 39020 0 .00580 20 65.1 0.0479 1360

14 1 13 .78 7 .07 54054 64364 0 .00420 20 47.6 0.0485 982 1110

14 2 9.93 11 .15 68514 64364 0 .00532 20 59.7 0.0485 123 0

28 1 15.93 9 .38 56125 43338 0 .00648 20 60.5 0.0492 1230 1120

28 2 10.09 9.25 34925 43338 0 .00403 20 49.3 0.0492 1000

35 1 6.22 18.80 40239 36580 0 .00550 20 52.0 0.0497 1050 1090

35 2 11 .61 14.68 45751 36580 0 .00625 20 56.2 0.0497 1130

60 1 10.47 12.76 52589 32770 0 .00802 20 81.7 0.0493 1660 1380

~ 60 2 8.59 15 .26 35857 32770 0 .00547 20 54.2 0.0493 1100

Concentration in fish body at steady state (28-60day) : 59.0 μg/g

Concentration in water at steady state (28-60day) : 0.0488 mg/L

BCF at steady state (BCFss) : 1210

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D=A=B XCstdXDRx (FV/1000)x SF=(W 1+W2)=(R/100)x 1000

F=A=Bx CstdxDRx(FV/1000)x SF-(W 1+W2)-(R/100)X 1000=E

G=(F1+F2)-2

Figure 9-1 Bioconcentration factor (BCF) - High concentration level (PeakA)

{

10000 ----------

------------ ------------ -------------------------------------

---------------------------------------------------------

-- ------------------------------------------------------

U 1000

-- ---------- -----------

--------------------------------=----------------------

---------------------------------------------------------

---------------------------------------------------------

100

0 7 14 21 28 35 42 49 56

Exposure period (day)

-42- k,:,g
p.12

Table 6-2 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- High concentration level (Peak B)

W1 W2 A B C DR D E F G

exposur fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.1 No.2 count solution body in water

day No. g sample std. mg/L μg/g mg/L

7 1 10.98 9.17 41601 39366 0.00528 20 62.0 0.0475 1310 1370

7 2 11.57 9.47 47458 39366 0 .00603 20 67.7 0.0475 143 0

14 1 13 .78 7.07 57332 65291 0.00439 20 49.8 0.0482 1030 1160

14 2 9.93 11 .15 72040 65291 0.00552 20 61.9 0.0482 1280

28 1 15 .93 9.38 59412 43935 0.00676 20 63.2 0.0490 1290 1170

28 2 10 .09 9.25 36957 43935 0.00421 20 51.6 0.0490 1050

35 1 6.22 18.80 42744 36858 • 0.00580 20 54.8 0.0495 1110 1160

35 2 11 .61 14.68 48853 36858 0.00663 20 59.6 0.0495 1200

60 1 10.47 12 .76 55704 . 32763 0.00850 20 86.5 0.0492 1760 1460

60 2 8.59 15.26 37789 32763 0.00577 20 57.2 0.0492 1160

Concentration in fish body at steady state (28-60day) : 62.1 pg/g

Concentration in water at steady state (28-60day) : 0.0489 mg/L

BCF at steady state (BCFss) : 1270

Concentration of std. solution (Cstd) : 0.005 rng/L

Final volume (FV)

Sampling factor (SF)

: 10ML

: 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D=A=BxCstdxDRx(FV/1000)x SF-* (W 1+W2)=(R/100)x 1000

F=A=B XCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000=E

G=(F1+F2)-2

Figure 9-2 Bioconcentration factor (BCF) - High concentration level (PeakB)

10000

F

---------

----------------- --------------------------------------

---------------------------- -----------------

-------

----------------------------------------------

U 1000 ------------------------

---- --------------------

100

0 7 14 21 28 35 42 49 56

Exposure period (day)
p. 13

Table 6-3 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- High concen tration level (Peak C)

W1 W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.1 No.2 count solution body in water

day No. g g sample std. mg/L 119/9 mg/L

7 1 10.98 9.17 37232 47074 0.00395 20 45.5 0.0492 924 972

7 2 11.57 9.47 42823 47074 0.00455 20 50.1 0.0492 1020

14 1 13.78 7.07 50898 79428 0.00320 20 35.6 0.0495 719 807

14 2 9.93 11 .15 63995 79428 0 .00403 20 44.3 0.0495 895

28 1 15.93 9.38 51919 53732 0.00483 20 44.2 0.0504 878 790

28 2 10.09 9.25 31730 53732 0.00295 20 35 .4 0.0504 702

35 1 6.22 18.80 37325 44963 0.00415 20 38 .4 0.0506 760 795

35 2 11.61 14.68 42819 44963 0.00476 20 42.0 0.0506 830

60 1 10.47 12 .76 48410 38267 0.00633 20 63 .1 0.0501 1260 1050

60 2 8.59 15.26 33297 38267 0 .00435 20 42.3 0.0501 844

Concentration in fish body at steady state (28-60day) : 44.2 μg/g

Concentration in water at steady state (28-60day) : 0.0496 mg/L

BCF at steady state (BCFss) : 891

Concentration of std . solution (Cstd) : O.OOSmg/I,

Final volume (FV) : lOm

: 100 L Sampling factor (SF) 0

Recovery (R) : 86.3%

Equations : C=A=BxCstd

D=A-. BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000

F=A=BxCstdxDRx(FV/1000)XSF=(W 1 +W2)=(R/100)x 1000=E

G=(F1+F2)-2

~

Figure 9-3 Bioconcentration factor (BCF) - High concentration level (PeakC)

10000

--------------------------------------------------------

--------------------------------------------------------

---------------------------------------------------------

--------------------------------------

1000 -- --- #-- _______----

~ _____~=======================

------------------------------

----------------------------------------------------------

----------------------------------------------------------

100 1

0 7 14 21 28 35 42 49 56

Exposure period (day)

-44- lo$ 3
p. 14

Table 6-4 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- High concentration level (Peak D)

W1 W2 A B C DR D E F G

exposure fish peak area cone. Dilution cone. mean BCF mean

period weight in final ratio in fish cone. BCF

No.1 No.2 count solution body in water

day No. g g sample std. mg/L μg/g mg/L

7 1 10.98 9.17 39862 52665 0.00378 20 43.5 0.0498 874 922

7 2 11.57 9.47 46175 52665 0.00438 20 48.3 0.0498 970

14 1 13.78 7 .07 53718 89576 0.00300 20 33.3 0.0500 667 744

14 2 9.93 11 .15 66801 89576 0 .00373 20 41 .0 0.0500 820

28 1 15.93 9.38 55755 59749 0 .00467 20 42.7 0.0507 843 758

28 2 10.09 9 .25 34047 59749 0.00285 20 34.1 0.0507 673

35 1 6.22 18.80 39928 49711 0.00402 20 37.2 0.0509 731 763

35 2 11.61 14.68 45662 49711 0.00459 20 40.5 0.0509 795 f

60 1 10.47 12.76 51033 43233 0.00590 20 58.9 0.0500 1180 988

60 2 8.59 15 .26 35429 43233 0.00410 20 39.8 0.0500 796

Concentration in fish body at steady state (28-60day) : 42.2 μg/g

Concentration in water at steady state (28-60day) : 0.0489 mg/L

BCF at steady state (BCFss) : 863

Concentration of std, solution (Cstd) : 0.0051ng/L

Final volume (FV) : I OmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=A+BxCstd

D =A=BXCstdXDRx FV/1000 XSF= Wl+W2 = R/100 x100 ( ) ( )( ) 0

F=A=B XCstdXDRx(FV/1000)x SF-(W 1+W2)=(R/100)X 1000=E

G=(F1+F2)-2

~

Figure 9-4 Bioconcentration factor (BCF) - High concentration level (PeakD)

10000 ------ -------- ------------------------

--------------------------------------------------- --------

-------- -----------------------------------------------------

--=--------------------------------------------------

----------------------------------------------------

----------------------------------------------------

w

1000

____-__-___~=========== --===~=====================

----------------------------------------------------

-------------------------------------------------------

100 '

0 7 14 21 28 35 42 49 56

Exposure period (day)
p. 15

Table 7-1 Concentration of the test substance in the test fish and bioconcentration factor (BCF)

- Low concentration level (Peak A )

W1 W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.l No.2 count solution body in water

day No. g g sample std. mg/L μg/g mg/L

7 1 14.70 7.81 49089 39020 0.00629 2 6.61 0.00480 1380 1290

7 2 10.82 9.36 38172 39020 0 .00489 2 5.73 0.00480 1190

14 1 7.97 10 .30 125720 64364 0.00977 1 6.32 0.00486 1300 1450

14 2 9.47 9.82 81146 64364 0.00630 2 7.73 0.00486 1590

28 1 14.30 7.65 47279 43338 0.00545 2 5.87 0.00488 1200 1200

28 2 10.44 12.83 49524 43338 0 .00571 2 5.80 0.00488 1190

35 1 6.50 17.00 43534 36580 0 .00595 2 5.99 0.00492 1220 1240

35 2 13.50 8.17 41325 36580 0.00565 2 6.16 0.00492 1250

60 1 11.91 12 .66 51321 32770 0.00783 2 7.53 0.00498 1510 1520

60 2 8.48 17.51 55046 32770 0.00840 2 7.64 0.00498 1530

Concentration in fish body at steady state (28-60day) : 6.50 μg/g

Concentration in water at steady state (28-60day) : 0.00506 mg/L

BCF at steady state (BCFss) : 1280

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV)

Sampling factor (SF)

: lOmL

: 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D =A=BxCstdxDRx(FV/1000)XSF=(W 1+W2)=(R/100)x 1000

F=A=BxCstdxDRX(FV/1000)xSF=(W1+W2)=(R/100)x 1000=E

G = (F I +F2)-2

Figure 10-1 Bioconcentration factor (BCF) - Low concentration level (Peak A)

10000

------------------------------------------------------------ --------------------

------------------------------------------------------------ ----------------------------------=-------------------------

----------------------------------------------------------

----------------------------------------------------------

--------------------------------------------------------------

------------------------------------------------ ----------

~ 1000

------------------

--------------------------------------------------------

----------------------------------------------------------

----------------------------------------------------------

--------------------------------------------------------------

100

0 7 14 21 28 35 42 49 56 .

Exposure period (day)
p. 16

Table 7-2 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- Low concentration level (Peak B )

W1 W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight infinal ratio in fish conc. BCF

No.l No.2 count solution body in water

day No. g g sample std. mg/L μg/g mg/L

7 1 14.70 7.81 51481 39366 0.00654 2 6.87 0.00479 1430 1350

7 2 10.82 9.36 40528 39366 0.00515 2 . 6.03 0.00479 1260

14 1 7.97 10.30 132535 65291 0.01015 1 6.57 0.00485 1350 1510

14 2 9.47 9.82 86205 65291 0.00660 2 8.09 0.00485 1670

28 1 14.30 7.65 49887 43935 0.00568 2 6.11 0.00489 1250 1250

28 2 10.44 12 .83 52763 43935 0.00600 2 6.10 0.00489 1250

35 1 6.50 17.00 45929 36858 0 .00623 2 6.27 0.00492 1270 1290

35 2 13 .50 8.17 43405 36858 0.00589 2 6.42 0.00492 131 0

( 60 1 11.91 12.66 54512 32763 0.00832 2 8.00 0.00496 1610 1630

60 2 8.48 17.51 58750 32763 0.00897 2 8.16 0.00496 1640

Concentration in fish body at steady state (28-60day) : 6 .84 μg/g

Concentration in water at steady state (28-60day) : 0.00504rng/L

BCF at steady state (BCFss) : 1360

Concentration of std. solution (Cstd) : 0.005 mg/L

Final volume

Sampling factor

(FV)

(SF)

: 10mL

: 1000

Recovery (R) : 84.6%

Equations : C=A=BXCstd

D=A=BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000

F=A=BXCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)x 1000=E

G=(F1+F2)-2

Figure 10-2 Bioconcentration factor (BCF) - Low concentration level (Peak B)

10000 ---- ---- --------- - - ---------- ------------------------

----------------------------------------------------------- ---------------------------------- -----------

-----------------------------------------------

----------------------------------------------------------

-------------------------------------------------------------

---------------------------------------------

--------------

U 1000 ---------------------------------------------------------

-------------------------------------- -----------

---------------------------------------------------

-------------------------------------------------------------

-------------------------------------------------------------

100 '

0 7 14 21 28 35 42 49 56

Exposure period (day)

~-' Co$
p. 17

Table 7-3 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- Low concentration level (Peak C )

WI W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.l No.2 count solution body in water

day No. g g sample std. mg/L μg/ mg/L

7 1 14.70 7.81 46491 47074 0.00494 2 5.08 0.00491 1040 971

7 2 10.82 9.36 36284 47074 0.00385 2 4.43 0.00491 901

14 1 7.97 10.30 113720 79428 0.00716 1 4.54 0.00494 919 1030

14 2 9.47 . 9.82 74927 79428 0 .00472 2 5.67 0.00494 1150

28 1 14.30 7.65 43885 53732 0.00408 2 4.31 0.00498 866 858

28 2 10.44 12.83 45620 53732 0.00425 2 4.23 0.00498 849

35 1 6.50 17.00 39303 44963 0.00437 2 4.31 0.00500 862 874

35 2 13 .50 8.17 37211 44963 0.00414 2 4.43 0.00500 885

60 1 11.91 12.66 46278 38267 0.00605 2 5.70 0.00507 1120 1140

60 2 8.48 17.51 50649 38267 0.00662 2 5.90 0.00507 1160

Concentration in fish body at steady state (28-60day) : 4.81 μ.g/g

Concentration in water at steady state (28-60day) : 0.00516 mg/L

BCF at steady state (BCFss) : 932

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV)

Sampling factor (SF)

: 10mL

: 1000

Recovery (R) : 86.3%

Equations : C=A=BXCstd

D=A=B xCstdxDRx(FV/1000)X SF=(W 1+W2)=(R/100)x 1000

F=A=B xCstdXDRx(FV/1000)x SF~(W 1+W2)=(R/ 100)x 1000=E

G=(F1+F2)-2

(

Figure 10-3 Bioconcentration factor (BCF) - Low concentration level (Peak C)

10000

-----------------------------------------------------------

-------------------------------------------

U 1000

_

------------------------------------------------------------

------------------------------------------------------------

------------------------------------------------------------

-------------------- --------------------------------------

100

0 7 14 21 28 35 42 49 56

Exposure period (day)
p. 18

Table 7-4 Concentration of the test substance in the test fish and bioconcentration factor (BCF )

- Low concentration level (Peak D)

W1 W2 A B C DR D E F G

exposure fish peak area conc. Dilution conc. mean BCF mean

period weight in final ratio in fish conc. BCF

No.1 No.2 count solution body in water

day No. g g sample std. mg/L [Ig/g mg/L

7 1 14.70 7.81 49968 52665 0 .00474 2 4.88 0.00495 987 924

7 2 10.82 9.36 39044 52665 0 .00371 2 4.26 0.00495 860

14 1 7.97 10.30 121296 89576 0.00677 1 4.29 0.00499 861 966

14 2 9.47 9.82 79668 89576 0.00445 2 5.34 0.00499 1070

28 1 14.30 7.65 46330 59749 0.00388 2 4.09 0.00499 820 816

28 2 10.44 12 .83 48573 59749 0.00406 2 4.05 0.00499 81 1

35 1 6.50 17.00 41724 49711 0 .00420 2 4.14 0.00502 824 837

35 2 13 .50 8.17 39640 49711 0 .00399 2 4.26 0.00502 849

60 1 11.91 12.66 49241 43233 0.00569 2 5.37 0.00504 1070 1090

60 2 8.48 17.51 53518 43233 0.00619 2 5.52 0.00504 1100

Concentration in fish body at steady state (28-60day) : 4.57 μg/g

Concentration in water at steady state (28-60day) : 0.00509 mg/L

BCF at steady state (BCFss) : 898

Concentration of std. solution (Cstd) : 0.005 mg/L

Final volume (FV)

Sampling factor (SF)

: lOmL

: 1000

Recovery (R) : 86.3

: C=A=BXCstd % Equations

D=A=B xCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000

F=A=BxCstdxDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000=E

G =(F 1+F2)-2

Figure 10-4 Bioconcentration factor (BCF) - Low concentration level (Peak D)

10000

w -----------------------------------------------------

U 1000

-----------------------------------------------------

-----------------------------------------------------

100 I

0 7 14 21 28 35 42 49 56

Exposure period (day )

-49- ~ocbg
P. 19

Table 8 Concentration of the test substance in the tissues and bioconcentration factor (BCF)

- High concentration level

Peak A

W A B C DR D E F

tissue tissue peak area conc. dilution conc . mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L μg/g mg/L

head 11.39 29352 19311 0.00760 10 78.9 0.0493 1600

viscera 5.52 24842 19311 0.00643 10 137.7 0.0493 2790

muscle & bones 16 .04 17470 19311 0.00452 10 33.3 0.0493 676

fins & skin (with scales) 4 .91 27284 19311 0.00706 5 85.0 0.0493 1720

Concentration of std. solution (Cstd) : 0.005 mg/L

Final volume (FV) : 10mL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Peak B

W A B C DR D E F

tissue tissue peak area conc. dilution conc. mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L μg/g mg/L

head 11.39 31321 19508 0.00803 10 83.3 0.0492 1690

viscera 5.52 26712 19508 0.00685 10 146.6 0.0492 2980

muscle & bones 16.04 18705 19508 0.00479 10 35.3 0.0492 718

fins & skin (with scales) 4 .91 28941 19508 0.00742 5 89.3 0.0492 1810

Concentration of std. solution (Cstd) : 0.005rng/L

Final volume (FV) : 10 mL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D=A=BXCstdxDRx(FV/1000)xSF=W=(R/100)x 100 0

F = A=B x CstdXDRx(FV/1000)xSF=W=(R/100)x 1000=E

~ ~n$°l
p. 20

Table 8 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- High concentration level (continued)

Peak C

W A B C DR D E F

tissue tissue peak area conc. dilution conc. mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L F,g/g mg/L

head 11.39 26838 23284 0.00576 10 58.6 0.0501 1170

viscera 5.52 22585 23284 0.00485 10 101 .8 0.0501 2030

muscle & bones 16.04 16023 23284 0.00344 10 24.9 0.0501 496

fins & skin (with scales) 4 .91 24140 23284 0 .00518 5 61.2 0.0501 1220

Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Peak D

W A B C DR D E F

tissue tissue peak area conc. dilution conc. mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L μg/g mg/L

head 11.39 28359 26217 0.00541 10 55.0 0.0500 1100

viscera 5.52 23912 26217 0.00456 10 95.7 0.0500 1910

muscle & bones 16.04 16944 26217 0.00323 10 23.3 0.0500 467

fins & skin (with scales) 4 .91 25758 26217 0 .00491 5 58.0 0.0500 1160

~ Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=ABXCstd

D=A=B xCstdxDRx(FV/1000)xSF=W=(R/100)x 1000

F=A=BxCstdxDRx(FV/1000)XSF=W=(R/100)x 1000=E

~- ~,~ d
p. 21

Table 9 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- Low concentration level

Peak A

W A B C DR D E F

tissue tissue peak area conc. dilution cone. mean BCF

weight in final ratio in fish cone.

count solution body in water

g sample 'std . mg/L' gg/g mg/L

head 10.76 33287 17953 0.00927 1 10.18 0.00498 2050

viscera 4.38 25620 17953 0.00714 1 19.26 0.00498 3870

muscle & bones 18 .88 22462 17953 0.00626 1 3.92 0.00498 786

fins & skin (with scales) 4 .49 10642 17953 0.00296 1 7.80 0.00498 1570

Concentration of std. solution (Cstd) : 0.005 mg/L

Final volume (FV) : 10mL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Peak B

W A B C DR D E F

tissue tissue peak area cone. dilution cone. mean BCF

weight in final ratio in fish conc,

count solution body in water

g sample std. mg/L μg/g mg/L

head 10.76 35457 17947 0.00988 1 10.85 0.00496 2190

viscera 4.38 27397 17947 0.00763 1 20.60 0.00496 4150

muscle & bones 18.88 23755 17947 0.00662 1 4.14 0.00496 835

fins & skin (with scales) 4 .49 11310 17947 0.00315 1 8.30 0.00496 1670

Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : l OmL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D= A=B x Cstd x DRx (FV/ 1000) x SF=W=(R/ 100) x 1000

FA=BXCstdxDRx(FV/1000)x SF=W=(R/100)X 1000=E
p. 22

Table 9 Concentration of the test substance in the tissues and bioconcentration factor (BCF )

- Low concentration level (continued)

Peak C

W A B C DR D E F

tissue tissue peak area conc. dilution conc. mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L μg/g mg/L

head 10.76 29935 21712 0.00689 1 7.42 0.00507 1460

viscera 4.38 22485 21712 0.00518 1 13.70 0.00507 2700

muscle & bones 18.88 20050 21712 0.00462 1 2.83 0.00507 559

fins & skin (with scales) 4 .49 9245 21712 0.00213 1 5.49 0.00507 1080

Concentration of std. solution (Cstd) : 0.005rng/L,

Final volume (FV) : l OmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Peak D

W A B C DR D E F

tissue tissue peak area conc. dilution conc. mean BCF

weight in final ratio in fish conc.

count solution body in water

g sample std. mg/L μg/g mg/L

head 10.76 31992 24272 0.00659 1 7.10 0.00504 1410

viscera 4.38 24243 24272 0.00499 1 13.21 0.00504 2620

muscle & bones 18.88 21285 24272 0.00438 1 2.69 0.00504 534

fins & skin (with scales) 4 .49 10083 24272 0.00208 1 5.36 0.00504 1060

{ Concentration of std. solution (Cstd) : 0.005rng/L

Final volume (FV) : I OmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=A=BxCstd

D=A=B xCstdxDRX(FV/ 1000) x SF=W=(R/100)x 1000

F=A=B XCstd XDRx(FV/1000) x SF=W=(R/ (R/1 x 1000=E
p. 23

Table 10-1 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak A)

W, WZ A B C DR D E

depuration fish peak area cone. Dilution conc . residual

period weight in final ratio in fish rate

No.l No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 59.0 100.0

1 1 16.65 9 .07 57757 32770 0.00881 10 40.5 71.9

1 2 10.19 11 .40 53146 32770 0.00811 10 44. 4

2 1 8.06 16.32 40792 31335 0 .00651 10 31 .6 59.0

2 2 11.38 11 .02 45132 31335 0.00720 10 38. 0

4 1 14.47 8.89 21525 21570 0.00499 10 25.2 30.8

4 2 10.67 10.08 16986 21570 0.00394 5 11.2

8 1 12.99 6.63 45761 25485 0.00898 1 5.4 9.1

8 2 11 .62 9.98 24670 25485 0.00484 2 5.3

Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D =A=B xCstdXDRx(FV/1000)XSF=(W 1+W2)=(R/100)x 1000

E = (Di 1+Di2)-2=Do x 10 0

Figure 11-1 Concentration of the test substance in the test fish during depuration test

- High concentration level (PeakA)

~ 100 ------- - - -- - -- - --- - - -- -------------------------------------- --- - ----- - ------- ---- - ----------- ----------------------------------------------------- ;~ ----------------------------------- ~ -- --------------------------- 03017x_ _

... ---- --- --------------------- y59 .084e

------------ -------------- ---- ----

---------------------- ~-------- RZ= 0 .9455 ----

1 0

~:. ------------------------------------------ --------

r -

--------------------------------------------- -----

-----------------------------------------------

L; -----------------------------------------------------

_____________________________________________________

O

-----------------------------------------------------

1

0 2 4 6 8

Depuration period (day)

BHL = 2.3 day
p. 24

Table 10-2 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak B )

WI W2 A B C DR D E

depuration fish peak area conc. Dilution conc. residual

period weight in final ratio in fish rate

No.l No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 62 .1 100.0

1 1 16.65 9.07 61845 32763 0.00944 10 43.4 73.4

1 2 10.19 11 .40 57190 32763 0 .00873 10 47. 8

2 1 8.06 16.32 44232 31757 0.00696 10 33.8 59.7

2 2 11.38 11 .02 48644 31757 0.00766 10 40. 4

4 1 14.47 8 .89 23602 21644 0.00545 10 27.6 32.3

4 2 10.67 10.08 19003 21644 0.00439 5 12. 5

r

~ 8 1 12.99 6 .63 53605 25894 0.01035 1 6.2 9.8

8 2 11.62 9.98 ., 28584 25894 0.00552 2 6. 0

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV) : l OmL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BXCstd

D=A=B xCstdxDRx(FV/1 000)x SF-, (W 1+W2)=(R/100)X 1000

E = (Di1+Di-,)-2=DoX 100

Figure 11-2 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak B)

100

--------------------------------------- - - - ------ ------------------ ------

---- ------ --------------------- y=62.414e"o.zszX

=L •

-

Rz=0.945 ----

------------------------- - - - - - - ---

o •

10

,~ ---------------------------------------------- ---- -------------------------------------------------

: -------------------------------------------------- 4- ---------- -- ----------------------- --------- ------------

----------------------------------------------------

----------------------------------------------------

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

O

0 2 4 6 8

Depuration period (day)

BHL = 2.4 day

-6-a- t~~ '-A
p. 25

Table 10-3 Concentration of the test substance in the test fish during depuration tes t

- High concentration level (Peak C )

W1 W_, A B C DR D E

depuration fish peak area conc. Dilution conc. residual

period weight in final ratio in fish rate

No.1 No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 44.2 100.0

1 1 16.65 9.07 49773 38267 0.00650 10 29.3 68.9

1 2 10.19 11 .40 45002 38267 0.00588 10 31 . 6

2 1 8.06 16.32 31948 38496 0.00415 10 19.7 50.5

2 2 11.38 11.02 37026 38496 0.00481 10 24. 9

4 1 14.47 8.89 16032 25618 0.00313 10 15.5 24.9

4 2 10.67 10.08 11925 25618 0.00233 5 6. 5

8 1 12.99 6.63 27475 30239 0.00454 1 2.7 6.1

8 2 11.62 9.98 15280 30239 0 .00253 2 2.7

Concentration of std. solution (Cstd) : 0.005rng/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=A=BXCstd

D =A=B XCstdxDRx(FV/1000)X SF=(W 1+W2)=(R/100)x 1000

E = (Di l+Di.))-2=Dox 100

Figure 11-3 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak C )

~

100 --------- ------------------------------------------------------ -----------------------=------------------------------

n -------------------------------------y=43 .268e .3a9sX : : : :

~ --------------------------------- ----

v R2=0 ----- ----------------------------- ----

.953 4

------------ ---------- ---------

o

~ 10 ----- - - ---- - -- - --- -- -- ----- --- ,~ ------------------------- -------------------- -------------------------- ------------------

w -------------------- - - - -

---------------------------- ------ -- ------------- -- -- --------

~ --------------------------------------------- ----- ------------------------------

---------------- ~~ ------------------------------------------------------

0

U

0 2 4 6 8

Depuration period (day)

BHL = 2 .0 day

we& - to °~ S
p. 26

Table 10-4 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak D)

W, WZ A B C DR D E

depuration fish peak area cone. Dilution cone. residual

period weight in final ratio in fish rate

No.l No.2 count solution body

day No. g g sample std. ing/L gg/g %

0 Concentration in fish body at steady state (28-60day) 42.2 100.0

I 1 16.65 9.07 51793 43233 0 .00599 10 27.0 67.2

1 2 10.19 11 .40 47830 43233 0.00553 10 29. 7

2 1 8.06 16.32 33016 42807 0.00386 10 18.3 48.8

2 2 11.38 11 .02 37819 42807 0.00442 10 22. 9

4 1 14.47 8.89 15931 28766 0.00277 10 13.7 22.9

~ 4 2 10.67 10.08 11611 28766 '0.00202 5 5. 6

8 1 12.99 6.63 24748 34439 0.00359 1 2.1 5.1

8 2 11.62 9.98 14267 34439 0.00207 2 2.2

Concentration of std. solution (Cstd) : 0.005mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=A=BxCstd

D =A=BxCstdXDRX(FV/1000)xSF=(W 1+W2)=(R/100)x 1000

• E=(Dil+Di2)-2=Dox100

Figure 11-4 Concentration of the test substance in the test fish during depuration test

- High concentration level (Peak D)

^ 100 ---------

- - - - -- -- - ---- -

----------------------------------------------------- ------------------------------------------------------

I

------

-------------- -

---------------------------------

--

--------- ------

0 2 4 6 8

Deputation period (day)

BHL = 1.9 day
p. 27

Table 11-1 Concentration of the test substance in the test fish during depuration tes t

- Low concentration level (Peak A)

W, W2 A B C DR D E

depuration fish peak area conc. Dilution conc. residual

period weight in final ratio in fish rate

No.l No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 6.50 100.0

1 1 15.07 7.75 40070 32770 0 .00611 1 3.17 52.8

1 2 10.94 11 .27 45433 32770 0.00693 1 3.69

2 1 12.88 8.04 38505 31335 0.00614 1 3.47 54.8

2 2 11.70 13 .97 49685 31335 0.00793 1 3.65

4 1 16.05 12.24 16915 21570 0.00392 1 1 .64 30.1

4 2 13.32 17.16 25219 21570 0.00585 1 2.27

8 1 13.88 13 .21 7820 25485 0.00153 1 0.67 14.6

8 2 12.18 11 .36 12466 25485 0 .00245 1 1 .23

Concentration of std. solution (Cstd) : 0.005rng/L

Final volume (FV) : 10mL

Sampling factor (SF) : 1000

Recovery (R) : 84.6%

Equations : C=A=BxCstd

D=A=B x CstdXDRx(FV/1000)x SF=(W 1+W2)=(R/100)x 1000

E = (D i l+DiZ)-2=Do x 100

Figure 12-1 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak A)

10 ------------------------------------------------------ -------------------- ----------------- ----------------- -------y5

---------------------------------- .0568e-0zi99X---==

---- ---- ---------------- ------ =L ------ ------- -------------------- R 0.8931 ----

--------------------------- -----------------------

~

_----------------------------------------------------

w

------------------------------------------------------

----------------------------------------------------

------------------------------------------------------

------------------------------------------------------

0-1

0 2 4 6 8

Depuration period (day)

BHL = 3.2 day
p. 28

Table 11-2 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak B )

W, W2 A B C DR D E

depuration fish peak area conc. Dilution conc. residual

period weight in final ratio in fish rate

No.l No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 6 .84 100.0

1 1 15.07 7.75 42873 32763 0.00654 1 3.39 53.7

1 2 10.94 11 .27 48620 32763 0.00742 1 3.95

2 1 12.88 8.04 41682 31757 0.00656 1 3.71 55.5

2 2 11.70 13.97 53487 31757 0.00842 1 3.8 8

4 1 16.05 12.24 18713 21644 0.00432 1 1.81 31.4

4 2 13.32 17.16 27725 21644 0.00640 1 2.48

8 1 13.88 13.21 8852 . 25894 0.00171 1 0.75 15.5

8 2 12.18 11.36 14084 25894 0.00272 1 1.37

Concentration of std . solution (Cstd) : 0.005mg/L

Final volume (FV)

Sampling factor (SF)

: lOmL

: 1000

Recovery (R) : 84.6%

Equations : C=A=BxCst d

D =A=BxCstdxDRx(FV/ 1000)x SF=(W 1+W2)=(R/100)x 1000

E=(Di1+Di2)+2=Dox 100

Figure 12-2 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak B)

10 ----- ----------- -- - - - -- - ,-. ------ - - - - ----------------------------------- \en ---------- ozi~~---- ----------------- ----Y=53506e--

------ •- ------- =L ----- -------- ----------------- RZ0.891

-------------------------- ~-- --------------------

41

--- -

- - - - - - - - - - - - = - - : - - - - - - - Z ----------- ---------

:: :: - - - - ------ ----- - - - 2 ::7 - -- - - - - - - Z :: - - -

~ ---------------------------- ------------------------

---------------------------------- -------

o -------------------- --------------------------------

. .. ---------------------------------------------------- !

----------------------------------------------------

----------------------------------------------------

U 0.1

0 2 4 6 8

Depuration period (day)

BHL = 3 .3 day
p. 29

Table 11-3 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak C )

W, W2 A B C DR D E

depuration fish peak area conc. Dilution conc. residual

period weight in final ratio in fish rate

No.1 No.2 count solution body

day No. g g sample std. mg/L gg/g %

0 Concentration in fish body at steady state (28-60day) 4 .81 100.0

1 1 15.07 7.75 33730 38267 0.00441 1 2.24 50.5

1 2 10.94 11.27 38405 38267 0.00502 1 2.62

2 1 12.88 8.04 30674 38496 0.00398 1 2.21 47.3

2 2 11.70 13.97 39954 38496 0.00519 1 2.34

4 1 16.05 12.24 12670 25618 0.00247 1 1.01 24.8

4 2 13.32 17.16 18584 25618 0.00363 1 1.3 8

8 1 13.88 13.21 5027 30239 0.00083 1 0.36 10.2

8 2 12.18 11.36 7653 30239 0.00127 1 0.62

Concentration of std . solution (Cstd) : 0.005rng/L

Final volume

Sampling factor

(FV)

(

: lOmL

SF) : 1000

Recovery (R) : 86.3%

Equations : C=A=BxCst d

D =A=BxCstdxDRx(FV/1000)xSF=(W 1+W2)=(R/100)X 1000

E= (Dil +Di2)-2=Dax 100

Figure 12-3 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak C)

10 - -

y 3

-- --- ------ -- -- - - - - - - - - - - - - - - - - - - - 0 2619x

------------------- =--Y=3.6582e '

en

R2= 0.9314

-- ---------------------------------------- `.

------ ------- -- --------------------------------------

0 1 ----- --- ----- -- ----------- ---------------------------------------------

. .

w~ -------------------- . ----- ------ ----- ------------------------------ G -------------------- ----------------------------------

-----------------------------------------------------------

------------------------------ ----------------------------

0

U 0.1

0 2 4 6 8

Depuration period (day)

BHL = 2.6 day
p. 30

Table 11-4 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak D)

Wl WZ A B C DR D E

depuration fish peak area cone. Dilution cone. residual

period weight in final ratio in fish rate

No.1 No.2 count solution body

day No. g g sample std. mg/L μg/g %

0 Concentration in fish body at steady state (28-60day) 4.57 100.0

1 1 15.07 7.75 35294 43233 0.00408 1 2.07 49.2

1 2 10.94 11 .27 40246 43233 0.00465 1 2.43

2 1 12.88 8 .04 31492 42807 0.00368 1 2.04 45.8

2 2 11 .70 13.97 40731 42807 0.00476 1 2.1 5

4 1 16.05 12.24 12723 28766 0.00221 1 0.91 23.5

4 2 13.32 17.16 18807 28766 0.00327 1 1 .24

8 1 13.88 13 .21 5029 34439 0.00073 1 0.31 9.3

8 2 12.18 11 .36 7552 34439 0.00110 1 0.54

Concentration of std . solution (Cstd) : 0.005 mg/L

Final volume (FV) : lOmL

Sampling factor (SF) : 1000

Recovery (R) : 86.3%

Equations : C=A=BxCstd

D =A=B x CstdxDRx(FV/ 1000)X SF=(W 1+W2)=(R/100)X 1000

E=(Di1+Di2)-2=DoX 10 0

Figure 12-4 Concentration of the test substance in the test fish during depuration test

- Low concentration level (Peak D)

10 -------------------------------------------------------

------------------------- - --- --- -----------

------------------------------------- -----

------------------- ------------------ -3y = - .4395e-o2rz4X

-----

~ R2 _0 -- ---------------------------------- .9336- -----

0

.fl

~ 1 -------------------------- ------- -------------------

~ ------------- v~ --------------------------------------- -------------

4; ----

- -

------------------------------

---------------------------------

- -

-

-

- -------- - - - --

- -

- - - - - :11

----------------------------------------------------

V _ _ _ _ _ _ _ _ --------------------------------------------- _

G

0 -------------------------------------------------------

U

0 .1

0 2 4 6

Deputation period (day)

BHI., = 2 .5 day

-6}- ~ o O
p. 31

Figure 1 Bioconcentration test system

tap water IF"

air

pump pump

- [Lr

0

0

0

feed solution dilution water

(24±2°C, D.O.>7mg/L) activated charcoal

(dechlorination) sodium

~ te

mixing tube thiosulfate solution

flon sheet

56L-glass aquarium

(50L water)

to waste treatment system

(Apparatus)

Pump (dilution water supply)

Exposure : FMI Co., QD-2CSC

Control: FMI Co., QD-2CSC

~ Pump (feed solution supply)

Exposure: Nihon Seimitsu Kagaku Co ., SP-D-2501(S)

Control : Nihon Seimitsu Kagaku Co., SP-T-2500 S

~~~~
p. 32

Figure 2-1 Calibration curve of the test substance (Peak A )

Input Data

No. Concentration Peak Area

1 0 0

(mg/L) (count)

2 0.0025 20496

3 0.005 41273

4 0.01 78797

Y= 7,970,OOOX

r= 0.9995 detection limit = 0.00025mg/L

80000 S

~

60000

C~.)

~..i

Sud 40000 !R'

~

20000 4V

~ 0 0

0

.0025 0.005 0.0075 0.01

Concentration (mg/L)
p. 33

Figure 2-2 Calibratiori curve of the test substance (Peak B )

Input Data

No. Concentration Peak Area

1 0 0

(mg/L) (count)

2 0.0025 20847

3 0.005 42104

4 0.01 79471

Y= 8,060,OOOX

r= 0.9993 detection limit = 0.00025mg/L

80000 .

60000

~ _. .

0

v

c,d 40000 S .

20000

0

l 0 0.0025 0.005 0.0075 0.0 1

t Concentration (mg/L)
p. 34

Figure 2-3 Calibration curve of the test substance (Peak C )

Input Data

No. Concentration Peak Area

1 0 0

(mg/L) (count)

2 0.0025 24313

3 0.005 49713

4 0.01 96272

Y= 9,690,OOOX

r= 0.9998 detection limit = 0.00025mg/L

100000

~

80000

0

60000 -

lt~

a~i •'

40000

~, .

20000

0

t 0 0.0025 0.005 0.0075 0.01

Concentration (mg/L)
p. 35

Figure 2-4 Calibration curve of the test substance (Peak D)

Input Data

No. Concentration Peak Area

1 0 0

(mg/L) (count)

2 0.0025 27773

3 0.005 56335

4 0.01 108311

Y= 10,930,000X

r= 0.9997 detection limit = 0.00025mg/L

~ 100000

80000

60000

N ~ I

.

Cd

40000

~, .

2000 0

~ 0 0

0

.0025 0.005 0.0075 0.01

Concentration (mg/L)
p. 36

SANITIZED
p. 37

Appendix 1

Information on the test substance

(2 pages)
p. 38

1•._ ~^e-~ ~ Lc~ ~-b s o,~ ~~ ~ o,r~ S e c~,-~

SAN ITIZED

k 3
p. 39

Appendix 2

~

Dilution water quality

(2 pages)

t
p. 40

Dilution water quality

Parameter Concentration

BOD <2.0 mg/L

COD <3

PH 8

.0 mg/L

.0 (20°C)

Oil N

Coliform group bacteria N. D

.D

Cadmium <0.01 mg/L

Cyanide N.D

Lead <0.1 mg/L

Chromium <0.05 mg/L

Arsenic <0.05 mg/L

Mercury <0.0005 mg/L

Free chlorine <0.02 mg/L

Bromide <1 .0 mg/L

Fluoride <1.5 mg/L

Sulfide <0.3 mg/L

Total ammonium <1.0 mg/L

Copper <0.005 mg/L

Zinc <0.1 mg/L

Aluminum <0.1 mg/L

Tin <1.0 mg/L

Manganese <1.0 mg/L

Iron <1.0 mg/L

Nickel <0.1 mg/L

Total phosphorus <0.1 mg/L

f. Selenium <0.001 mg/L

Phenols <0.002 mg/L

Anionic surfactant <0.02 mg/L

Evaporation residue 88 mg/L

Electric conductivity 131 ~c S/cm

Total hardness (as CaCO3) 49 mg/L

Alkalinity 29 mg/L

Sodium 8 .8 mg/L

Potassium 1.1 mg/L

Calcium 14 mg/L

Magnesium 3.6 mg/L

sampling date : February 6, 2006
p. 41

Appendix 3

Chromatograms (Analysis of the test substance in the test water)

(10 pages)

~-~`~
p. 42

C- V\~..r o V"\,~o vo`w`S

SANITIZED
p. 43

Appendix 4

Chromatograms (Analysis of the test substance in the test fish during exposure period)

(11 pages)

(

-4-7 2 ~
p. 44

SANITIZED
p. 45

Appendix 5

Chromatograms (Analysis of the test substance in the tissues)

(5 pages)

~

}

~ Z ~~
p. 46

SANITIZED

3~- -I L-A ~
p. 47

Appendix . 6

Chromatograms (Analysis of the test substance in the test fish during depuration period)

(9 pages)

~

-~- -7`A
p. 48

SANITIZED

m .k

~~ ^1 4 3
p. 49

.03srTMAZI

-~

. ...

td

cn

td

t7.

E

O

U