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For Experimental Use Only

For use only at an application site of a cooperator or participant and in 

accordance with the terms and conditions of the Experimental Use Permit. Not for 

sale to any person other than a participant or cooperator of the EPA-approved 

Experimental Use Permit program. This label must be in possession of the user at 

the time of pesticide application. For use only in the state of California.

Read safety directions before using.

Wolbachia pipientis, wAlbB

Strain

in male Aedes aegypti WB1 strain mosquitoes

EPA Experimental Use Permit No. 88877-EUP-2

ACTIVE INGREDIENT:

Wolbachia pipientis, wAlbB Strain*:...........................100.00%

OTHER INGREDIENTS:........................................................0.00% 

Total:.................................................................................100.00% *The Active Ingredient is Wolbachia pipientis, wAlbB Strain, intracellular, obligate, and naturally-occurring bacteria in male Aedes aegypti WB1 strain

mosquitoes. 

KEEP OUT OF REACH OF CHILDREN

CAUTION

Net contents: 0.236 oz. (6.7 g) maximum adult male Aedes aegypti mosquitoes, with each 

mosquito containing approximately 0.001% Wolbachia pipientis, wAlbB Strain. 

EPA Experimental Use Permit No.: 88877-EUP-2. Lot # _______

Expiration of male mosquito batch date: 24 hours after receipt of shipping container,

except if male mosquitoes are put in cages and fed sucrose upon receipt of the 

shipping container, then they may be kept for up to 3 days prior to release.

EPA Est. Nos.: 88877-KY-001 and/or 89668-KY-001 

University of Kentucky, Department of Entomology

S-225 Agricultural Science Center, North

Lexington, KY 40546-0091

(859) 257-4902 Office

(859) 257-5117 Lab

(859) 323-1120 Fax

e-mail: sdobson@email.uky.edu

88877-EUP-2

10/15/2015
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PRECAUTIONARY STATEMENTS

ENVIRONMENTAL HAZARDS 

Do not contaminate water when disposing of equipment washwater or rinsate. 

DIRECTIONS FOR USE

FOR EXPERIMENTAL USE ONLY 

It is a violation of Federal law to use this product in a manner inconsistent with its 

labeling.

For experimental use by The University of Kentucky, Department of Entomology; the 

Consolidated Mosquito Abatement District of Selma; and their cooperators in California 

under the EPA Experimental Use Permit (EUP) for control of Aedes aegypti mosquitoes. 

Only male (and no female) Ae. aegypti strain WB1 mosquitoes carrying Wolbachia 

pipientis, wAlbB Strain microbial pesticide are to be released. The mosquitoes and their 

life stages are for use only by the staff, technicians, and/or cooperators of The University 

of Kentucky, Department of Entomology and the Consolidated Mosquito Abatement 

District of Selma specifically trained for this purpose. Before making the first applications 

under this EUP, consult with the state agency with primary responsibility for pesticide 

regulation to determine if other regulatory requirements exist. Releases of male 

mosquitoes are done on a weekly basis with three releases per week, totaling ~100,000 

adult male mosquitoes containing W. pipientis, wAlbB Strain. Male mosquitoes are 

released to the air and fly away to mate in the air. All of the use sites for this EUP are 

included in Section G of the application (on Field Trial Information Requirements Forms).

All mosquito rearing is done at EPA Est. Nos. 88877-KY-001 and/or 89668-KY-001. 

100,000 Ae. aegypti males that carry W. pipientis, wAlbB Strain microbial pesticide are to 

be released per week for up to six months in 2015 and 2016 at the above identified sites.

To measure for population control effects, mosquito populations will be sampled using 

mosquito traps to monitor adults and eggs. These mosquitoes typically lay eggs in 

containers, such as discarded cups or bottles.

Mosquitoes are reared in water from a municipal source in plastic pans at ~82.4F (~28°C).

Mosquito larvae eat various foods including powdered liver, cat or dog food, or fish food. 

Immature mosquitoes are kept at a density of approximately one larva/ml. The 

mosquitoes become pupae in about five days and develop in the pupal stage for two days

before separation of the smaller male pupae from larger female pupae by a mechanical 

separator and by visual sorting. This device separates the smaller male pupae from 

larger female pupae by the difference in size. The female pupae are killed by freezing

and, when dead, put in a Ziploc®-type PE bag and disposed of with trash and/or garbage.

Male mosquitoes are allowed to eclose or emerge as adult males and groups of adult 

males are visually examined to remove any residual adult females. The visual sorting is 

repeated a second time prior to shipping, for a total of two visual sorting examinations. 

Male mosquitoes for release are immobilized by chilling ((59ÛF aÛ&) for delivery to the 

release site. They are placed in multiple small plastic containers in a sealable PE bag and 

then put into an insulated shipping container with common reusable ice cooler packs.

At the release site, adult male mosquitoes are placed on a platform and exposed to 

ambient natural conditions. After acclimation, they become active and fly away to 

disperse and mate with indigenous Ae. aegypti female mosquitoes.
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STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

PESTICIDE 

STORAGE

Keep male mosquitoes chilled with ice packs at ~59ÛF (~Û&) during 

delivery to the release site. If male mosquitoes are put in cages and fed 

sucrose upon receipt of the shipping container at the release site, then 

they may be kept for up to 3 days prior to release.

PESTICIDE 

DISPOSAL

Unwanted female Ae. aegypti mosquitoes are killed by freezing, then 

put in a Ziploc®-type PE bag and disposed of with trash and/or garbage.

At the conclusion of the experimental field trial program, all mosquito 

colony life stages maintained for the EPA-approved Experimental 

Program are to be frozen to kill them, then put in a Ziploc®-type PE bag 

and disposed of with trash and/or garbage. All equipment used in the 

rearing process (e.g., cages and larval pans) are to be washed with a 

commercial disinfectant cleaner.

CONTAINER 

HANDLING

Any durable containers that can be reused are to be washed with a 

commercial disinfectant cleaner. Disposable containers, including 

plastic containers and bags, are to be rinsed with a commercial 

disinfectant cleaner to make sure no viable mosquito life stages are 

present and recycled or disposed of in trash.