## Breadcrumb

-  Publications 
-  Articles 

#           Pesticides in small volume plasma samples: Method development and application to smallmouth bass (Micropterus dolomieu) from the Chesapeake Bay watershed, USA      

      February 3, 2024    

Nontarget organisms are exposed to pesticides following applications in agricultural and urban settings, potentially resulting in deleterious effects. Direct measurements of pesticides in biological tissues may aid in characterizing exposure, accumulation, and potential toxicity versus analyses in environmental media alone (e.g., water, soil, and air). Plasma represents a nonlethal sampling medium that can be used to assess recent exposures to contaminants. Herein, a method was developed to test the extraction of 210 pesticides and their transformation products in small volume plasma samples (100 μL). Plasma samples were protein precipitated with 0.5 % formic acid in acetonitrile added to the sample (ratio of 3.5:1). Pass-through solid phase extraction was used for sample matrix and lipid removal and samples were analyzed by liquid chromatography and gas chromatography with tandem mass spectrometry. Recoveries of 70.0–129.8 % were achieved for 182 pesticides and degradates across the low (25 ng mL−1), medium (100 ng mL−1), and high (250 ng mL−1) spike levels. Method detection levels ranged 0.4–13.0 ng mL−1. Following development, the method was applied to smallmouth bass (Micropterus dolomieu) plasma samples (n = 10) collected from adults in the Chesapeake Bay watershed. Individual plasma samples resulted in four to seven analytes detected with summed concentrations ranging 16.4–95.0 ng mL−1. Biological multiresidue pesticide methods help elucidate recent exposures of bioactive compounds to nontarget organisms.

## Citation Information

## Related Content

                  -              Data           
                  -              Connect           

January 29, 2024

####                 Pesticides in small volume plasma samples              

California Water Science Center

####             Michael S Gross (Former Employee)          

##### Research Chemist

California Water Science Center

#### Vicki S. Blazer

#####  Research Fishery Biologist

Eastern Ecological Science Center

vblazer@usgs.gov

304-724-4434

#### Michelle L Hladik, PhD

##### Research Chemist

California Water Science Center

mhladik@usgs.gov

916-278-3183

## Related Content

January 29, 2024

####                 Pesticides in small volume plasma samples              

California Water Science Center

####             Michael S Gross (Former Employee)          

##### Research Chemist

California Water Science Center

#### Vicki S. Blazer

#####  Research Fishery Biologist

Eastern Ecological Science Center

vblazer@usgs.gov

304-724-4434

#### Michelle L Hladik, PhD

##### Research Chemist

California Water Science Center

mhladik@usgs.gov

916-278-3183

### Study Area

### Contact

####               Sacramento Projects Office (USGS California Water Science Center)          

6000 J Street, Placer HallSacramento, CA95819United States

916-278-3000

916-278-3070

####               Eastern Ecological Science Center at the Leetown Research Laboratory          

11649 Leetown RoadKearneysville, WV25430United States

### Explore Search

                  - Biology
                  - United States
                  - chesapeake bay watershed

View All **