# Sustainable Futures

**Summary Assessment**

**Using P2 Framework Models** 

**Chemical A ssessed**  **:** 4,7 - Methano - 1H - indene - 1 - propanal, octahydro - and 4,7 - Methano - 1H - indene - 2 - propanal, octahydro 

## CA**S Registr y**  **Number:** [1464989 - 16 - 9 and 1467039 - 69 - 5] 

## Participant **Name: Paul Sterchele**  **Senior Regulatory**

**Toxicologist Global Regulatory** 

**A ffairs**  **International Flavors & Fragrances Inc.**  

## Date of **As sessment:**  **December 2014** This document was developed to help compile estimation results from U.S. EPA OPPT’s P2 Fram ework Models and is used by OPPT during Sustainable Futures (SF) training described at www.epa.gov/oppt/sf . Participants in the voluntary Sustainable Futures Program are asked to submit the information contained in this assessment along with their SF PMNs in their choice of format. **Use of this specific format is not mandatory.** 2 **Record ID: CAS**  **No.** 1464989 - 16 - 9 & 1467039 - 69 - 5 **Chemical Structure**

+

(60

– 70 %) * 

### O (15 - 25%) **Is this a representative structure? Yes**  **MW :**  192.15 **MF:** C13H20O **Physical Form:** Liquid **Submitter:** International Flavors & Fragrances Inc. **Trade Name:** To Be D etermined **Use:** Fragrance Ingredient **Production Volume:**  1000 kg/yr (Projected

– non -US Manufacturing) **SMILES:** O=CCCC2CCC3C1CCC(C1)C23 & O=CCCC2CC3C1CCC(C1)C3(C2) **Name:** 4,7 -Methano - 1H - indene - 1 -propanal, octahydro - & 4,7 -Methano - 1H - indene - 2 -propanal, octahydro **Synonyms:** None 

### * Structure of primary isomer /constituent was used for completing the S ustainable F utures summary assessment , as the minor isomer modeling values produced similar output as the primary constituent. O3 **SUSTAINABLE FUTURES SUMMARY: Concern Level**  **HIGH MOD**  **ERATE LOW**  **Persistence** XXX **Bioconcentration** XXX **Cancer Health Hazard** Low -Moderate **Non -**  **Cancer Health Hazard** XXX **Aquatic Toxicity Hazard** XXX **Is the chemical predicted to be a PBT by PBT Profiler?** No

**Overall Hazard Concern Human Health Hazard:**  Low **Aquatic Hazard:** High **Overall Risk Human Health Risk:**  Low **Aquatic Risk:** Low4 **CAS No. 1464989**  **- 16**  **- 9**  **Submitter: International Flavors & Fragrances Inc.**  **PHYSICAL/CHEMICAL PROPERTIES: Melting Point (deg C)**  < - 82 (< 191K) (OECD 102, IFF 2014) 1 Attachment ID# 007 **Boiling Point (deg C)** 269.89 (EPISuite v4.11) **Vapor Pressure (mm Hg)** 8.4 x 10 - 3 @ 20°C; 1.4 x 10 - 2 @ 25°C (OECD 104 , IFF 2014 ) 1 Attachment ID# 007 **Water Solubility (g/L)** 0.0134 at 20°C (OECD 105 , IFF 2014 ) 1 Attachment ID# 007 **Octanol/W ater Partition Coefficient**  **- Log K** **ow** 3.9

– 4.8 (OECD 117, IFF 2014) 1 Attachment ID# 007 **ENVIRONMENTAL TRANSPORT AND FATE:**

**Transport Henry’s Law Constant**  **– HLC (atm**  **- m**  **3 /mol)**  1.169 x 10 - 4 (EPISuite v4.11) **Soil Adsorption Coefficient –**  **Log K oc**  2.591 (MCI method) or 3.351 (Kow method) (EPISuite v4.11) **Bioconcentration Factor –**  **BC F**  **(L/kg wet -** **wt)** 682.4 (EPISuite v4.11) **Persistence Probability of Rapid Biodegradation**  Yes (EPISuite v4.11) **Ultimate Biodeg Model** 2.7965 weeks (EPISuite v4.11) **Primary Biodeg M odel**  3.7669 days (EPISuite v4.11) **Ready Biodegradability (MITI Model)**  Yes (EPISuite v4.11) **Atmospheric Half**

**- life**  3.332 hrs (EPISuite v4.11) **Hydrolysis Half**

**- life**  Cannot be estimated (EPISuite v4.11) **Volatilization Half**

**- life for Model River**  3.376 days (8 1.01 hrs) (EPISuite v4.11) **Volatilization Half**

**- life for Model Lake**  41.67 days (1000 hrs) (EPISuite v4.11) **Removal in Sewage Treatment Plant*** Total predicted removal is 70.44% (EPISuite v4.11) **Experimental Data** Ready biodegradation test (OECD 301D, IFF 2 014), NOT readily biodegradable under the test conditions with 8.48% degradation after 28 days. 2 Attachment ID# 0 14 **Byproducts Degradation Products**  Not measured or analyzed **Metabolites** Not measured or analyzed * Unless experimental data indica te otherwise, the maximum value used by EPA will be 9 5 % removal.5 **CAS No. 1464989**  **- 16**  **- 9**  **Submitter: International Flavors & Fragrances Inc.**  **ECOTOXICITY: ECOSAR Class**  Aldehydes (Mono) **Acute Toxicity**

**Fish LC 5O**  0.376 mg/L, 96 -hr 

### (ECOSAR v1.11) Experimental D ata (Draft report) : The L C 50 (96 -hr) of the test substance in zebra - fish species was 3.525 mg/L based on no minal concentrations , and 0. 913 mg/L based on the geometric means of measured concentrations. 3 Attachment ID# 0 21

**Daphnid LC 5O**  0. 150 mg/L , 48 -hr (E COSAR v1.11) No experimental data. Read across to analog* CAS 86803 - 90 - 9. D aphnia magna Acute Toxicity, 199 5 . Research Institute for Fragrance Materials (RIFM ) Database L ocation 30033, Sub -Reference 04/04 4

. Under the conditions of the study, conducted according to OECD Guideline s for Testing of Chemicals (1984) No. 202 "Acute Immobilisation Test and Reproduction Test" per Method C.2 of Commission Directive 92/69/EEC (Annex V of Council Directive 67/548/EEC), the 48 hour EC 50 for the test material = 5.5 mg/l (95% confidence limits = 4.5

– 6.7 mg/L ) . Result details: An Acute Toxicity study was conducted on 20 Daphnia ma gna/concentration for 48 hours under static conditions at test concentrations of: 1, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L . The NOEL was determined to be 1.8 mg/L and the EC 50 for 48 hours wa s calculated to be 5.5 mg/L .

**Green Algae EC 5O**  0.432 mg/L , 96 -hr (ECOSAR v1.11) No experimental data. Read across to analog* CAS 86803 - 90 - 9. Algae Inhibition Test, 199 5 . Research Institute for Fragrance Materials (RIFM ) Database Location 30034 , Sub -Reference 05 /04 5

. Under the conditions of the study, conducted according to OECD Guideline s for Testing of Chemicals (1984) No. 20 1 "Alga Growth Inhibition Test" per Method C.3 of Commission Directive 92/69/EEC (Annex V of Council6 Directive 67/548/EEC), the EbC 50 value (biomass reduction at 72 hours) was determined to be 1 mg/L , and the ErC 50 value (growth rate reduction at 24

– 48 hours) was determined to be 1.7 mg/L . Result details: The effect of the test substance on growth inhibition was studied in green algae exposed for up to 72 hours to test concentrations of: 0.625, 1.25, 2.5, 5.0, and 10 mg/L . The NOEL was determined to be 0.246 mg/L (based on measured test concentrations ), the EbC 50 value = 1 mg/L , and the ErC 50 value = 1.7 mg/L . **Chronic Toxicity Fish ChV**  0.019 mg/L (ECOSAR v1.11) **Daphnid ChV** 0.037 mg/L (ECOSAR v1.11) **Green Algae ChV** 0.289 mg/L (ECOSAR v1.11) **Overall Hazard Concern for Aquatic Toxicity** Based on the predicted acute and chronic values for aquatic toxicity, the aquatic hazard concern is high. However, based on the ratios of the Acute PEC to Acute COC, and the ratio s of Chronic PEC to Chronic COC , the risk to the aquatic environment is expected to be low. See Worksheet s #2 and #3 below for details. **Low est Chronic COC (lowest ChV/10)**  **– used as input**  **to determine Aquatic Exposure in E -** **FAST** 2 ppb **CANCER HEALTH EFFECTS: Experimental data**  None **OncoLogic Results** The level of concern when the anticipated route of exposure is inhalation is MARGINAL. The level of concern when the anticipated route of exposure is dermal is LOW-MODERATE. **Overall Hazard Concern for Carcinogenicity** LOW **NON -**  **CANCER HEALTH EFFECTS: Acute Toxicity**  No data. Read across to analog* CAS 86803 - 90 - 9 1) Acute Dermal Toxicity Study in Rabbits, 1985 (RIFM Location 30022, Sub - Reference11/01) 6 Under the conditions of the study, conducted according to Annex V of the EEC Commission Directive, **the dermal**  **LD 50**  **for the test material is greater than 2000**  **mg/kg/day** . Study details: An acute dermal limit toxicity study was conducted with five male and five female, New Zealand white rabbits. A single dose of 2000 mg/kg/day of undiluted test material was applied on the dorsal skin under semi - occlusive patches for 24 hou rs. Observations for systemic effects were made at7 frequent intervals (at least hourly for the first five hours) after dosing and then daily for 14 days after. All animals were sacrificed after 14 days and examined for gross pathology. Irritation was evaluated as part of the gross necropsy. No systemic effects or macroscopic changes, other than skin irritation , were observed. The test substance had no toxic effect when applied to the skin of the rabbit. The results of this study demonstrate that the LD 50 o f the test substance on single application to the skin of the rabbit is > than 2000 mg/kg/day. 2) Acute Oral Toxicity Study in Rat s , 1989 (RIFM Location 30021, Sub - Reference 11/22) 7 Under the conditions of the study, the **oral LD**  **50 for the test material is**  **estimated to be 2800 mg/kg/day.**  Study details: An acute oral toxicity study was conducted for 14 days on six Sprague - Dawley rats, three males and three females. The rats were fasted for 24 hours before test material was administered by oral gavage dosing at the following concentrations: Dose (mg/kg bw) Effect 625 No effects 1250 No effects 2500 2 of 6 dead; Lethal 2800 Calculated LD 50

( 95% confidence interval of 21 00 to 376 0 mg/kg) 5000 6 of 6 dead; Lethal The animal’s weights were rec orded right after dosing and after the 7th and 14th day. All animals were observed for signs of illness and death for the entire study period. **Irritation** Modified Shelanski - Shelanski Human Patch Test (HRIPT), IFF 201 4. The PMN substance FRET 09 - 0536 - 01 did **NOT** demonstrate a potential for eliciting dermal **irritation** at 2 % under occlusive patch test conditions. 8 Attachment ID# 09 **Skin Sensitizer** Modified Shelanski - Shelanski Human Patch Test (HRIPT), IFF 201 4 . The PMN substance FRET 09 - 0536 - 01 did **NOT** demonstrate a potential for eliciting dermal **sensitization** at 2 % under the occlusive patch test conditions. 8 Attachment ID# 098 KeratinoSens Assay, IFF 2014. The PMN substance Fragrance Research Chemical TM 09 - 0536 - 01 was predicted to be a non - sensitizer in this in vitro cell culture system. 9 Attachment ID# 010 Direct Peptide Reactivity Assay , IFF 2014. The PMN substance Fragrance Research Chemical TM 09 - 0536 - 01 was predicted to be a potential sensitizer based on its low reactivity with the peptides in this in vitro system. 10 Attachment ID# 011 **Reproductive Effects** No Data Available **Developmental Effects** No Data Available **Immune System Effects** No Data Available **Neurotoxicity** No Data Available **Genotoxicity** The PMN substance FRET 09 - 0536 was non - clas togenic to human lymphocytes in vitro under the conditions of a c hromosome aberration t est , following Test Guideline OECD 473. 11 Attachment ID# 12 **Mutagenicity** The PMN substance FRET 09 - 0536 was non - mutagenic under the conditions of a b acterial reverse mutation test in strains of Salmonella typhimurium and Escherichia coli , following Test Guideline OECD 471. 12 Attachment ID# 13 **Systemic Effects** No data. Read across to analog* CAS 86803 - 90 - 9 4 Week, Subchronic (Gavage) Toxicity Study in the Rat (RIFM Location 30030, Sub - Reference 12/13 ) 13 Under the conditions of the study, the **Lowest -**  **Observed**   ** Effect Level (LOEL)** for the test material is **500 mg/kg/day**  . There were no treatment -related changes observed with respect to survival, bodyweight gain, hematol ogy, food consumption or macroscopic findings. However, absolute and relative liver weights were statistically increased in all treated female groups relative to controls, leading to the conclusion that the No Observed Effect Level is < 500 mg/kg/day. Study details: A 4 week toxicity study was conducted on rats (male and female). Three groups of 5 rats per dose received the test material administered once daily via oral gavage for the length of the study , at dose s of 500, 750 or 1000 mg/kg/day. A fourth group consisted of control animals that were administered vehicle . Daily observations and weekly body weights and food consumptions were recorded throughout the study. Blood was collected at the end of week four. **Overall Hazard Concern for Non -**  **Cancer H**  **ealth Effects** LOW9



### Note: ***analog :**  **4,7 -**  **Methano -**  **1H -**  **indene -**  **2 -**  **carboxaldehyde, octahydro -**  **5 -**  **methoxy****** **CAS# 86803**  **- 90**  **- 9**  O O **CAS No. 1464989**  **- 16**  **- 9**  **Submitter: International Flavors & Fragrances Inc.**  **INDUSTRIAL OPERATIONS INFORMATI ON**  **Operation Name** Fragrance oil compounding **Number of sites** 1 **Location** 

### International Flavors & Fragrances Inc . 600 State Highway

#36, Hazlet, New Jersey 07730 **Operating Days Per Year**  250 (maximum ; based

on estimation of 2 kg/batch, 2 batch/day) **INDUSTRIAL RELEASE SUMMARY (ChemSTEER)**  **Media Operation**  **Daily Release**  **(kg/site -**  **day) Release** 

**Days per**  **Year No of Sites** 

**of Release**  **Total Annual**

**Releases (kg/year**  **- all sites)**  **Release site information**

**(NPDES No. or SIC Code)**

Landfill Equipment

cleaning wastewater on - site treatment (sludge) 5.6E - 3 250 1 1.4 NPDES #NJ 0108707/NJ0021016 Passaic Valley Sewerage Commissions (PVSC)

Water Equipment

cleaning at IFF 2.3E - 3 250 1 0.58 NPDES #NJ0024708 Bayshore Regional Sewerage Authority (BRSA) Cleaning drums used to transport raw material at drum reconditioning contractor ’s site 0.62 5 1 3 .1 NPDES#: NJ0020141 The Middlesex County Utility Authority

Air Equipment

cleaning at IFF 1.9E -4 250 1 0.048 SI C 2844

– Soaps, detergent, etc. manufacture Sampling prod uct at IFF 2. 8 E - 8 250 1 7.0 E - 6 SI C 2844

– Soaps, detergent, etc. manufacture10

Cleaning drums used to transport raw material at drum reconditioning contractor ’s site 9.7E - 9 5 1 4. 9 E - 8 SI C 5093

– Scrap and Waste Materials Unloading raw material from drums at IFF 5. 8 E - 8 250 1 1.4E - 5 SI C 2844

– Soaps, detergent, etc. manufacture Loading product into containers at IFF 7.2E - 8 250 1 1.8E - 5 SI C 2844

– Soaps, detergent, etc. manufacture Equipment cleaning wastewater on - site treatment (stripping to air) 1.9E -4 250 1 0.048 SI C 2844

– Soaps, detergent, etc. manufacture

**OCCUPATIONAL EXPOSURE SUMMARY Operation**  **Route Potential**  **Dose Rate (mg/day)**  **Days/yr Workers**  **Cancer LADD**  **(mg/kg -**  **d ay)**  **Chronic**

**ADD (mg/kg**  **- day)**  **Acute**

**APDR (mg/kg**  **- day)**  Cleaning drums used to transport raw material Inhalation

(Worst case) 1.40E -4 5 1 1.6E - 8 2.7E - 8 2.0E - 6 Dermal (High end) 1. 8E3 6 1 0.2 4 0.41 25 .0 Sampling product Inhalation

(Worst case) 4.0E - 3 250 1 2.2E - 5 3.9E - 5 5.7E - 5 Dermal (High end) 8.82 250 1 0.049 0.086 0.1 30 Unloading liquid raw material from drums Inhalation

(Worst case) 8.3E - 3 250 1 4. 7 E - 5 8. 2 E - 5 1. 2 E -4 Dermal (High end) 1. 8E3 250 1 9. 9 17.0 25. 0 Loading liquid product into containers Inhalation

(Worst case) 0.01 250 1 5.8E - 5 1.0E -4 1. 5 E -4 Dermal (High end) 1 8.0 250 1 0.099 0.17 0 0.25 0 **Total number of Workers –**  **All Sites** 4511 **GENERAL POPULATION EXPOSURE SUMMARY (E**  **- FAST)**  **Cancer LADDpot (mg/kg/day)**  **L AD**  **C pot**  **Acute ADRpot (mg/kg/day)**  **Drinking Water Drum Cleaning**  3.16E - 7 2. 43 E - 5 mg/L Not Applicable

**Vessel Clean ing**  5.87E - 8 4.52E - 6 mg/L Not Applicable **Fish Ingestion Drum Cleaning**  2.12 E - 8 2.26E -4 mg/kg 2.72 E -4

**Vessel Cleaning** 7.48 E - 9 7.98E - 5 mg/kg 2.61 E - 6 **Fugitive Emissions**  **Drum Cleaning** 3.41E - 2 0.19 mg/m3 1.00

**Vessel Cleaning** 3.41E - 2 0.19 mg/m3 1.00 **Liquid Sampling**  3.41E - 2 0.19 mg/m3 1.00 **Unloading Liquid Material from Drums**  3.41E - 2 0.19 mg/m3 1.00 **Loading Liquid Product into Containers**  3.41E - 2 0.19 mg/m3 1.00 **Dermal –**  **Consumer Use General Purpose** 

**Cleaner** 2.58E - 3 Not Applicable 1.07E - 1 **Laundry Deterg ent**  2.58E - 3 Not Applicable 1.07E - 1 **Bar Soap** 1.64E - 2 Not Applicable 6.15E - 1 **Inhalation –**  **Consumer Use General Purpose** 

**Cleaner** 1.35E - 2 7.30E - 2 mg/m3 6.42E - 1 **Laundry Detergent** 3.94E -4 1.89E - 3 mg/m3 7.05E - 2

**AQUATIC EXPOSURE SUMMARY Low**  **est Acute COC –**  **Aq uatic Exposure**  30 ppb (Daphnid) **Low est Chronic COC**  **– Aquatic Exposure**  2 ppb (Fish) **Predicted Environmental Concentration (Acute PEC)** 0.11 ppb **Predicted Environmental Concentration (Chronic PEC)** 0.0571 ppb **PEC Exceeds Chronic COC (days / year)** 0 days **RISK ASSESSMENT CALCULATIONS: Acute Risk to Aquatic Organisms**  **LOW concern.**  Based on ratio of Acute PEC to Acute COC. See below Worksheet #1 for details. **Chronic Risk to Aquatic Organisms LOW**  **concern.** Based on ratio of Chronic PEC to Chronic COC. See below Worksheet #2 for details.

**MOE –**  **Acute Occupational Exposure LOW concern**  **.** Dermal and Oral LD 50 values are > 50 mg/kg for an analog material ( 4,7 -Methano - 1H - indene - 2 - carboxaldehyde, octahydro - 5 - methoxy - ; CAS# 86803 - 90 - 9). When acute toxicity studie s indicate LD 50 values are > 50 mg/kg for a chemical, there is no need to calculate MOE for acute risk, and it poses a low concern based on SF Assessment Guidance. Given the fact that all workers at the IFF facility are required to have personal protecti on equipment such as chemical resistant gloves and safety glasses, preventing direct dermal contact with the notified substance, the risk is12 anticipated to be **Low** . Furthermore, the production process of the notified substance is always performed with exhau st ventilation, minimizing the inhalation exposure.

**MOE –**  **Chronic Occupational Exposure POTENTIAL**  **concern if not wearing PPE. LOW concern with proper PPE.**  Based on ChemSTEER estimated LADD values for inhalation and dermal exposure to the notified materi al, and read across to LOEL value for systemic effects of the analog material 4,7 -Methano - 1H - indene - 2 - carboxaldehyde, octahydro - 5 - methoxy - (CAS# 86803 - 90 - 9) . S ee below Worksheet #3 for details. Considering all workers at the IFF facility are required to wear personal protection equipment such as chemical resistant gloves and safety glasses, thus preventing direct dermal contact with the notified substance, the anticipated risk is **LOW** . In addition, the production process of the notified substance is alway s performed with exhaust ventilation, minimizing the inhalation exposure as well.

**MOE –**  **Acute General Population Exposure LOW concern**  . Dermal and Oral LD 50 values are > 50 mg/kg for an analog material ( 4,7 -Methano - 1H - indene - 2 - carboxaldehyde, octahydro - 5 - methoxy - ; CAS# 86803 - 90 - 9). When acute toxicity studies indicate LD 50 values are > 50 mg/kg for a chemical, there is no need to calculate MOE for acute risk, and it poses a low concern based on SF Assessment Guidance. **MOE –**  **Chronic General Population E xposure**  **LOW concern** . Based on LADDpot for all exposure scenarios and routes combined and LOEL for systemic effects of analog material ( 4,7 -Methano - 1H - indene - 2 - carboxaldehyde, octahydro - 5 - methoxy - ; CAS# 86803 - 90 - 9). See below Worksheet #3 for details. 

###  **Worksheet#1: Identification of Acute Risk to Aquatic Organisms: Acute Endpoint**  **Value Factor**  **Acute COC PEC**  **Potential for Risk?**  Fish LC50 0.376 ppm 5 76 ppb 0.11 ppb **No** Daphnia LC50 0.150 ppm 5 30 ppb 0.11 ppb **No** Algae EC50 0 . 432 ppm 4 108 ppb 0.11 ppb **No**



###  **Worksheet#2: Identification of Chronic Risk to Aquatic Organisms: Chronic**  **Endpoint Value**  **Factor Chronic**  **COC PEC**  **Potential for Risk?**  Fish ChV 0.019 ppm 10 2 ppb 0.0571 ppb **No** Daphnia ChV 0.037 ppm 10 4 ppb 0.0571 ppb **No** Algae ChV 0.289 ppm 10 29 ppb 0 .0571 ppb **No**13



###  **Worksheet #3: Identification of Non -**  **Cancer Human Health Risk Effect**  **L OEL**  **Type of**

**Exposure Exposure**  **MOE Potential for Risk?**  Systemic effects [ 

### Read across to analog* CAS 86803 - 90 - 9] 500

mg/kg/ day Worker (cleaning drums) 0.414 mg/kg/day

Ch emS TEER ADD (dermal + inhalation, mainly dermal) 1208 **L ow** **concern** ***** Worker (unloading raw material from drums) 17.26 mg/kg/day

ChemS TEER ADD (dermal + inhalation, mainly dermal) 29 Yes (without PPE); **Low c**  **oncern (with proper PPE)** * Worker (sampling product) 0.086 mg/kg/day

ChemSTEER ADD (dermal + inhalation) 5814 **Low concern**  Worker (loading product into containers) 0.17 3 mg/kg/day

ChemS TEER ADD (dermal + inhalation, mainly dermal) 2890 **L ow** **c oncern**  **General Population**  0.206 mg/kg/day

E -FAST ADDp ot ( all routes) 2428 **Low concern**  *The exposure is mainly from dermal contact, which is a high - end estimate from the EPA/OPPT 2 -Hand Dermal Contact with Liquid Model. Considering workers at both IFF and the drum collection contractor are required to wear chemical resistant gloves and other personal protective equipment (PPE), actual dermal exposure of the material is expected to be minimal , if any. Potential risk concern is expected to be low with proper PPE.14 **References** 

### 1) Determination of Physi c o - Chemical Properties of FRET 09 - 0536. IFF Test Substance FRET 09 - 0536, IFF 201 4 . Study

attached as ID# 007 . 2) Ready

Biodegradability T est of FRET 09 - 0536. IFF Test Substance FRET 09 - 0536, IFF 201 4 . Study attached as ID# 0 14 . 3) Draft Report: Fish, Acute Toxicity Tes t for FRET 09 - 0536. IFF Test Substance FRET 09 - 0536, IFF 2014. Study

attached as ID# 0 21 . 4) Acute Toxicity

in Daphnia magna . 19 9 5 . RIFM Location 30033, Sub - Reference 04/04 . 5) Algae Inhibition Test. 19 9 5 . RIFM Location 3003 4 , Sub - Reference 0 5 /04 . 6) Acute Dermal Toxicity Study in Rabbits. 1985. RIFM Location 30022, Sub - Reference 11/01. 7) Acute Oral Toxicity

Study in Rats. 1989. RIFM Location 30021, Sub - Reference 11/22. 8) Repeated Insult Patch Test (RIPT)

– Shelanski Method. IFF Test Substance s FRET 09 - 0536 - 01 and FRET 09 - 0536 - 02 , IFF 2014. Study attached as ID# 09 . 9) Induction of Antioxidant- Response - Element Dependent Gene Activity And Cy totoxicity (Using MTT) in the Keratinocy

te ARE - Reporter Cel l Line Keratinosens. IFF Test S ubstance Research Fragrance Chemical TM 0 9 - 0536, IFF 2014. Study attached ID# 010 . 10) Sensitization Screen Using the Direct Peptide Reactivity Assay (DPRA). IFF Test Substance TM09 - 0536, IFF 201 4 . Study attached ID# 01 1 . 11) Evaluation of the Ability of FRET 09 - 0536 to Induce Chromosome Aberrations in Cultured Peripheral Human Lymphocy tes (With Repeat Experiment). IFF Test Substance FRET 09 - 0536, IFF 201 4 . Study

attached ID# 12 . 12) Evaluation of the Mutagenic Activity of FRET 09 - 0536 in the Salmonella Typhimurium Reverse Mutation Assay

and the Eschericia Coli Reverse Mutation Assay (With Independent Repeat). IFF Test Substance FRET 09 - 0536, IFF 201 4 . Study attached ID# 13 . 13) 

### 4 - Week Oral (Gavage) Toxicity Study of Methoxy

Dicyclopentadiene Carboxaldehyde in the Rat. 1996. RIFM Location 30030, Sub - Referenc e 12/13.