# Patent

## Title
Pyrazolyl guanidine F1F0-ATPase inhibitors and therapeutic uses thereof

## Abstract

The invention provides pyrazolyl guanidine compounds that inhibit F1Fo-ATPase, and methods of using pyrazolyl guanidine compounds as therapeutic agents to treat medical disorders, such as an immune disorder, inflammatory condition, or cancer.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 13/992,255 filed Aug. 15, 2013, which is the national stage of International Patent Application Serial No. PCT/US2011/063950 filed Dec. 8, 2011, which claims the benefit of and priority to U.S. Provisional Patent Application Ser. No. 61/420,950, filed Dec. 8, 2010, the entire contents of each application are hereby incorporated by reference.


FIELD OF THE INVENTION
The invention provides inhibitors of F1F0-ATPases (e.g., mitochondrial F1F0-ATPases) and their therapeutic use. In particular, the invention provides pyrazolyl guanidine compounds that inhibit F1F0-ATPase, and methods of using pyrazolyl guanidine compounds as therapeutic agents to treat a number of medical conditions.
BACKGROUND
Multicellular organisms exert precise control over cell number. A balance between cell proliferation and cell death achieves this homeostasis. Cell death occurs in nearly every type of vertebrate cell via necrosis or through a suicidal form of cell death, known as apoptosis. Apoptosis is triggered by a variety of extracellular and intracellular signals that engage a common, genetically programmed death mechanism.
Multicellular organisms use apoptosis to instruct damaged or unnecessary cells to destroy themselves for the good of the organism. Control of the apoptotic process therefore is very important to normal development, for example, fetal development of fingers and toes requires the controlled removal, by apoptosis, of excess interconnecting tissues, as does the formation of neural synapses within the brain. Similarly, controlled apoptosis is responsible for the sloughing off of the inner lining of the uterus (the endometrium) at the start of menstruation. While apoptosis plays an important role in tissue sculpting and normal cellular maintenance, it is also a component of the primary defense against cells and invaders (e.g., viruses) which threaten the well being of the organism.
Not surprisingly many diseases are associated with dysregulation of apoptotic cell death. Experimental models have established a cause-effect relationship between aberrant apoptotic regulation and the pathagenicity of various neoplastic, autoimmune and viral diseases. For instance, in the cell-mediated immune response, effector cells (e.g., cytotoxic T lymphocytes “CTLs”) destroy virus-infected cells by inducing the infected cells to undergo apoptosis. The organism subsequently relies on the apoptotic process to destroy the effector cells when they are no longer needed. Autoimmunity is normally prevented by the CTLs inducing apoptosis in each other and even in themselves. Defects in this process are associated with a variety of immune diseases such as lupus erythematosus and rheumatoid arthritis.
Multicellular organisms also use apoptosis to instruct cells with damaged nucleic acids (e.g., DNA) to destroy themselves prior to becoming cancerous. Some cancer-causing viruses overcome this safeguard by reprogramming infected (transformed) cells to abort the normal apoptotic process. For example, several human papilloma viruses (HPVs) have been implicated in causing cervical cancer by suppressing the apoptotic removal of transformed cells by producing a protein (E6) which inactivates the p53 apoptosis promoter. Similarly, the Epstein-Barr virus (EBV), the causative agent of mononucleosis and Burkitt's lymphoma, reprograms infected cells to produce proteins that prevent normal apoptotic removal of the aberrant cells thus allowing the cancerous cells to proliferate and to spread throughout the organism.
Still other viruses destructively manipulate a cell's apoptotic machinery without directly resulting in the development of a cancer. For example, destruction of the immune system in individuals infected with the human immunodeficiency virus (HIV) is thought to progress through infected CD4+ T cells (about 1 in 100,000) instructing uninfected sister cells to undergo apoptosis.
Some cancers that arise by non-viral means have also developed mechanisms to escape destruction by apoptosis. Melanoma cells, for instance, avoid apoptosis by inhibiting the expression of the gene encoding Apaf-1. Other cancer cells, especially lung and colon cancer cells, secrete high levels of soluble decoy molecules that inhibit the initiation of CTL mediated clearance of aberrant cells. Faulty regulation of the apoptotic machinery has also been implicated in various degenerative conditions and vascular diseases.
Controlled regulation of the apoptotic process and its cellular machinery is important to the survival of multicellular organisms. Typically, the biochemical changes that occur in a cell instructed to undergo apoptosis occur in an orderly procession. However, as shown above, flawed regulation of apoptosis can cause serious deleterious effects in the organism.
The need exists for improved compositions and methods for regulating the apoptotic processes in subjects afflicted with diseases and conditions characterized by faulty regulation of these processes (e.g., viral infections, hyperproliferative autoimmune disorders, chronic inflammatory conditions, and cancers). The present invention addresses this need and provides other related advantages.
SUMMARY
The invention provides pyrazolyl guanidine compounds that inhibit F1F0-ATPase (e.g., mitochondrial F1F0-ATPase), pharmaceutical compositions comprising pyrazolyl guanidine compounds, and methods of using such compounds and pharmaceutical compositions to treat a number of medical conditions. Accordingly, one aspect of the invention provides a family of compounds represented by Formula I:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein the variables are as defined in the detailed description.
Another aspect of the invention provides a family of compounds represented by Formula II:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein the variables are as defined in the detailed description.
The foregoing compounds can be present in a pharmaceutical composition comprising a compound described herein and a pharmaceutically acceptable carrier.
Another aspect of the invention provides a method of treating a subject suffering from a medical disorder. The method comprises administering to the subject a therapeutically effective amount of one or more pyrazolyl guanidine compounds described herein, e.g., a compound of Formula I or II, in order to ameliorate a symptom of the disorder. A large number of disorders can be treated using the pyrazolyl guanidine compounds described herein.
For example, the compounds described herein can be used to treat an immune disorder or inflammatory disorder, such as rheumatoid arthritis, psoriasis, chronic graft-versus-host disease, acute graft-versus-host disease, Crohn's disease, inflammatory bowel disease, multiple sclerosis, systemic lupus erythematosus, Celiac Sprue, idiopathic thrombocytopenic thrombotic purpura, myasthenia gravis, Sjogren's syndrome, scleroderma, ulcerative colitis, asthma, epidermal hyperplasia, and other medical disorders described herein. The compounds described herein can also be used to treat a cardiovascular disease, myeloma, lymphoma, cancer, or bacterial infection.
Another aspect of the invention provides a method of inhibiting an F1F0-ATPase, for example, a mitochondrial F1F0-ATPase. The method comprises exposing the F1F0-ATPase to a compound described herein, such as a compound of Formula I or II, to inhibit said F1F0-ATPase.


DETAILED DESCRIPTION OF THE INVENTION
The invention provides pyrazolyl guanidine compounds that inhibit F1F0-ATPase (e.g., mitochondrial F1F0-ATPase), pharmaceutical compositions comprising the pyrazolyl guanidine compounds, and methods of using the pyrazolyl guanidine compounds and pharmaceutical compositions in therapy.
Exemplary compositions and methods of the present invention are described in more detail in the following sections: I. Modulators of F1F0-ATPase Activity; II. Pyrazolyl Guanidine Compounds; III. Therapeutic Applications of Pyrazolyl Guanidine Compounds, and IV. Pharmaceutical Compositions, Formulations, and Exemplary Administration Routes and Dosing Considerations. Aspects of the invention described in one particular section are not to be limited to any particular section.
The practice of the present invention employs, unless otherwise indicated, conventional techniques of organic chemistry, pharmacology, molecular biology (including recombinant techniques), cell biology, biochemistry, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature, such as “Comprehensive Organic Synthesis” (B. M. Trost & I. Fleming, eds., 1991-1992); “Molecular cloning: a laboratory manual” Second Edition (Sambrook et al., 1989); “Oligonucleotide synthesis” (M. J. Gait, ed., 1984); “Animal cell culture” (R. I. Freshney, ed., 1987); the series “Methods in enzymology” (Academic Press, Inc.); “Handbook of experimental immunology” (D. M. Weir & C. C. Blackwell, eds.); “Gene transfer vectors for mammalian cells” (J. M. Miller & M. P. Calos, eds., 1987); “Current protocols in molecular biology” (F. M. Ausubel et al., eds., 1987, and periodic updates); “PCR: the polymerase chain reaction” (Mullis et al., eds., 1994); and “Current protocols in immunology” (J. E. Coligan et al., eds., 1991), each of which is herein incorporated by reference in its entirety.
To facilitate an understanding of the present invention, a number of terms and phrases are defined below.
As used herein, the term “guanidine” refers to a compound having the following core structure:




including pharmaceutically acceptable salt forms.

The term “alkyl” refers to a saturated straight or branched hydrocarbon, such as a straight or branched group of 1-12, 1-10, or 1-6 carbon atoms, referred to herein as C1-C12alkyl, C1-C10alkyl, and C1-C6alkyl, respectively. Exemplary alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, 2-methyl-1-propyl, 2-methyl-2-propyl, 2-methyl-1-butyl, 3-methyl-1-butyl, 2-methyl-3-butyl, 2,2-dimethyl-1-propyl, 2-methyl-1-pentyl, 3-methyl-1-pentyl, 4-methyl-1-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 2,2-dimethyl-1-butyl, 3,3-dimethyl-1-butyl, 2-ethyl-1-butyl, butyl, isobutyl, t-butyl, pentyl, isopentyl, neopentyl, hexyl, heptyl, octyl, etc.
The term “alkylene” refers to a diradical of an alkyl group. Exemplary alkylene groups include —CH2— and —CH2CH2—.
The term “haloalkyl” refers to an alkyl group that is substituted with at least one halogen. For example, —CH2F, —CHF2, —CF3, —CH2CF3, —CF2CF3, and the like.
The term “hydroxyalkyl” refers to an alkyl group that is substituted with at least one hydroxyl group. In certain embodiments, the hydroxyalkyl group is an alkyl group that is substituted with one hydroxyl group.
The term “cycloalkyl” refers to a monovalent saturated cyclic, bicyclic, or bridged cyclic (e.g., adamantyl) hydrocarbon group of 3-12, 3-8, 4-8, or 4-6 carbons, referred to herein, e.g., as “C4-8cycloalkyl,” derived from a cycloalkane. Exemplary cycloalkyl groups include cyclohexyl, cyclopentyl, cyclobutyl, and cyclopropyl.
The term “cycloalkylene” refers to a divalent (i.e., diradical) saturated cyclic, bicyclic, or bridged cyclic (e.g., adamantyl) hydrocarbon group of 3-12, 3-8, 4-8, or 4-6 carbons, referred to herein, e.g., as “C4-8cycloalkylene,” derived from a cycloalkane. Unless specified otherwise, the cycloalkylene may be substituted at one or more ring positions with, for example, halogen, azide, alkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, hydroxyl, alkoxyl, amino, nitro, sulfhydryl, imino, amido, carboxylic acid, —C(O)alkyl, —CO2alkyl, carbonyl, carboxyl, alkylthio, sulfonyl, sulfonamido, sulfonamide, ketone, aldehyde, ester, heterocyclyl, aryl, heteroaryl, —CF3, —CN, or the like. In certain embodiments, the cycloalkylene group is substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, hydroxyl, alkoxyl, and amino. In certain other embodiments, the cycloalkylene group is not substituted, i.e., it is unsubstituted. Exemplary cycloalkylene groups include




The term “aralkyl” refers to an alkyl group substituted with an aryl group.
The term “heteroaralkyl” refers to an alkyl group substituted with a heteroaryl group.
The term “alkenyl” refers to an unsaturated straight or branched hydrocarbon having at least one carbon-carbon double bond, such as a straight or branched group of 2-12, 2-10, or 2-6 carbon atoms, referred to herein as C2-C12alkenyl, C2-C10alkenyl, and C2-C6alkenyl, respectively. Exemplary alkenyl groups include, but are not limited to, vinyl, allyl, butenyl, pentenyl, hexenyl, butadienyl, pentadienyl, hexadienyl, 2-ethylhexenyl, 2-propyl-2-butenyl, 4-(2-methyl-3-butene)-pentenyl, etc.
The term “alkynyl” as used herein refers to an unsaturated straight or branched hydrocarbon having at least one carbon-carbon triple bond, such as a straight or branched group of 2-12, 2-8, or 2-6 carbon atoms, referred to herein as C2-C12alkynyl, C2-C8alkynyl, and C2-C6alkynyl, respectively. Exemplary alkynyl groups include, but are not limited to, ethynyl, propynyl, butynyl, pentynyl, hexynyl, methylpropynyl, 4-methyl-1-butynyl, 4-propyl-2-pentynyl, and 4-butyl-2-hexynyl, etc.
The term “aryl” is art-recognized and refers to a carbocyclic aromatic group. Representative aryl groups include phenyl, naphthyl, anthracenyl, and the like. Unless specified otherwise, the aromatic ring may be substituted at one or more ring positions with, for example, halogen, azide, alkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, hydroxyl, alkoxyl, amino, nitro, sulfhydryl, imino, amido, carboxylic acid, —C(O)alkyl, —CO2alkyl, carbonyl, carboxyl, alkylthio, sulfonyl, sulfonamido, sulfonamide, ketone, aldehyde, ester, heterocyclyl, heteroaryl, —CF3, —CN, or the like. The term “aryl” also includes polycyclic ring systems having two or more carbocyclic rings in which two or more carbons are common to two adjoining rings (the rings are “fused rings”) wherein at least one of the rings is aromatic, and the other ring(s) may be, for example, cycloalkyl, cycloalkenyl, cycloalkynyl, and/or aryl. The term “haloaryl” refers to an aryl group that is substituted with at least one halogen. In certain embodiments, the aromatic group is not substituted, i.e., it is unsubstituted.
The term “phenylene” refers to a multivalent radical (e.g., a divalent or trivalent radical) of benzene. To illustrate, a divalent valent radical of benzene is illustrated by the formula




The terms “heterocyclyl” or “heterocyclic group” are art-recognized and refer to saturated, partially unsaturated, or aromatic 3- to 10-membered ring structures, alternatively 3- to 7-membered rings, whose ring structures include one to four heteroatoms, such as nitrogen, oxygen, and sulfur. Heterocycles may also be mono-, bi-, or other multi-cyclic ring systems. A heterocycle may be fused to one or more aryl, partially unsaturated, or saturated rings. Heterocyclyl groups include, for example, biotinyl, chromenyl, dihydrofuryl, dihydroindolyl, dihydropyranyl, dihydrothienyl, dithiazolyl, homopiperidinyl, imidazolidinyl, isoquinolyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, oxolanyl, oxazolidinyl, phenoxanthenyl, piperazinyl, piperidinyl, pyranyl, pyrazolidinyl, pyrazolinyl, pyridyl, pyrimidinyl, pyrrolidinyl, pyrrolidin-2-onyl, pyrrolinyl, tetrahydrofuryl, tetrahydroisoquinolyl, tetrahydropyranyl, tetrahydroquinolyl, thiazolidinyl, thiolanyl, thiomorpholinyl, thiopyranyl, xanthenyl, lactones, lactams such as azetidinones and pyrrolidinones, sultams, sultones, and the like. Unless specified otherwise, the heterocyclic ring is optionally substituted at one or more positions with substituents such as alkanoyl, alkoxy, alkyl, alkenyl, alkynyl, amido, amidino, amino, aryl, arylalkyl, azido, carbamate, carbonate, carboxy, cyano, cycloalkyl, ester, ether, formyl, halogen, haloalkyl, heteroaryl, heterocyclyl, hydroxyl, imino, ketone, nitro, phosphate, phosphonato, phosphinato, sulfate, sulfide, sulfonamido, sulfonyl and thiocarbonyl. In certain embodiments, the heterocyclcyl group is not substituted, i.e., it is unsubstituted.
The term “heteroaryl” is art-recognized and refers to aromatic groups that include at least one ring heteroatom. In certain instances, a heteroaryl group contains 1, 2, 3, or 4 ring heteroatoms. Representative examples of heteroaryl groups includes pyrrolyl, furanyl, thiophenyl, imidazolyl, oxazolyl, thiazolyl, triazolyl, pyrazolyl, pyridinyl, pyrazinyl, pyridazinyl and pyrimidinyl, and the like. Unless specified otherwise, the heteroaryl ring may be substituted at one or more ring positions with, for example, halogen, azide, alkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, hydroxyl, alkoxyl, amino, nitro, sulfhydryl, imino, amido, carboxylic acid, —C(O)alkyl, —CO2alkyl, carbonyl, carboxyl, alkylthio, sulfonyl, sulfonamido, sulfonamide, ketone, aldehyde, ester, heterocyclyl, aryl, —CF3, —CN, or the like. The term “heteroaryl” also includes polycyclic ring systems having two or more rings in which two or more carbons are common to two adjoining rings (the rings are “fused rings”) wherein at least one of the rings is heteroaromatic, and the other ring(s) may be, for example, cycloalkyl, cycloalkenyl, cycloalkynyl, and/or aryl.
The term “heteroarylene” refers to a multi-valent (e.g., di-valent or trivalent) aromatic group that comprises at least one ring heteroatom. An exemplary “heteroarylene” is pyridinylene, which is a multi-valent radical of pyridine. For example, a divalent radical of pyridine is illustrated by the formula




The terms ortho, meta and para are art-recognized and refer to 1,2-, 1,3- and 1,4-disubstituted benzenes, respectively. For example, the names 1,2-dimethylbenzene and ortho-dimethylbenzene are synonymous.
The terms “amine” and “amino” are art-recognized and refer to both unsubstituted and substituted amines, e.g., a moiety that may be represented by the general formula:




wherein R50 and R51 each independently represent hydrogen, alkyl, alkenyl, or —(CH2)m—R61; or R50 and R51, taken together with the N atom to which they are attached complete a heterocycle having from 4 to 8 atoms in the ring structure; wherein R61 is aryl, cycloalkyl, cycloalkenyl, a heterocycle or a polycycle; and m is zero or an integer in the range of 1 to 8. In certain embodiments, R50 and R51 each independently represent hydrogen or alkyl.

The terms “alkoxyl” or “alkoxy” are art-recognized and refer to an alkyl group, as defined above, having an oxygen radical attached thereto. Representative alkoxyl groups include methoxy, ethoxy, propyloxy, tert-butoxy and the like. An “ether” is two hydrocarbons covalently linked by an oxygen. Accordingly, the substituent of an alkyl that renders that alkyl an ether is or resembles an alkoxyl, such as may be represented by one of —O-alkyl, —O-alkenyl, —O-alkynyl, —O—(CH2)m—R61, where m and R61 are described above.
The term “amide” or “amido” as used herein refers to a radical of the form —RaC(O)N(Rb)—, —RaC(O)N(Rb)Rc—, —C(O)NRbRc, or —C(O)NH2, wherein Ra, Rb and Rc are each independently selected from alkoxy, alkyl, alkenyl, alkynyl, amide, amino, aryl, arylalkyl, carbamate, cycloalkyl, ester, ether, formyl, halogen, haloalkyl, heteroaryl, heterocyclyl, hydrogen, hydroxyl, ketone, and nitro. The amide can be attached to another group through the carbon, the nitrogen, Rb, Rc, or Ra. The amide also may be cyclic, for example Rb and Rc, Ra and Rb, or Ra and Rc may be joined to form a 3- to 12-membered ring, such as a 3- to 10-membered ring or a 5- to 6-membered ring. The term “carboxamido” refers to the structure —C(O)NRbRc.
The term “sulfonamide” or “sulfonamido” as used herein refers to a radical having the structure —N(Rr)—S(O)2—Rs— or —S(O)2—N(Rr)Rs, where Rr, and Rs can be, for example, hydrogen, alkyl, aryl, cycloalkyl, and heterocyclyl. Exemplary sulfonamides include alkylsulfonamides (e.g., where Rs is alkyl), arylsulfonamides (e.g., where Rs is aryl), cycloalkyl sulfonamides (e.g., where Rs is cycloalkyl), and heterocyclyl sulfonamides (e.g., where Rs is heterocyclyl), etc.
The term “sulfonyl” as used herein refers to a radical having the structure RuSO2—, where Ru can be alkyl, aryl, cycloalkyl, and heterocyclyl, e.g., alkylsulfonyl. The term “alkylsulfonyl” as used herein refers to an alkyl group attached to a sulfonyl group.
The symbol “” indicates a point of attachment.
The compounds of the disclosure may contain one or more chiral centers and/or double bonds and, therefore, exist as stereoisomers, such as geometric isomers, enantiomers or diastereomers. The term “stereoisomers” when used herein consist of all geometric isomers, enantiomers or diastereomers. These compounds may be designated by the symbols “R” or “S,” depending on the configuration of substituents around the stereogenic carbon atom. Stereoisomers include enantiomers and diastereomers. Mixtures of enantiomers or diastereomers may be designated “(±)” in nomenclature, but the skilled artisan will recognize that a structure may denote a chiral center implicitly. Unless indicated otherwise, generic chemical structures and graphical representations of specific compounds encompass all stereoisomers.
Individual stereoisomers of compounds of the present invention can be prepared synthetically from commercially available starting materials that contain asymmetric or stereogenic centers, or by preparation of racemic mixtures followed by resolution methods well known to those of ordinary skill in the art. These methods of resolution are exemplified by (1) attachment of a mixture of enantiomers to a chiral auxiliary, separation of the resulting mixture of diastereomers by recrystallization or chromatography and liberation of the optically pure product from the auxiliary, (2) salt formation employing an optically active resolving agent, or (3) direct separation of the mixture of optical enantiomers on chiral chromatographic columns. Stereoisomeric mixtures can also be resolved into their component stereoisomers by well known methods, such as chiral-phase gas chromatography, chiral-phase high performance liquid chromatography, crystallizing the compound as a chiral salt complex, or crystallizing the compound in a chiral solvent. Stereoisomers can also be obtained from stereomerically-pure intermediates, reagents, and catalysts by well known asymmetric synthetic methods.
Geometric isomers can also exist in the compounds of the present invention. The symbol — denotes a bond that may be a single, double or triple bond as described herein. The present invention encompasses the various geometric isomers and mixtures thereof resulting from the arrangement of substituents around a carbon-carbon double bond or arrangement of substituents around a carbocyclic ring. Substituents around a carbon-carbon double bond are designated as being in the “Z” or “E” configuration wherein the terms “Z” and “E” are used in accordance with IUPAC standards. Unless otherwise specified, structures depicting double bonds encompass both the “E” and “Z” isomers.
Substituents around a carbon-carbon double bond alternatively can be referred to as “cis” or “trans,” where “cis” represents substituents on the same side of the double bond and “trans” represents substituents on opposite sides of the double bond. The arrangement of substituents around a carbocyclic ring are designated as “cis” or “trans.” The term “cis” represents substituents on the same side of the plane of the ring and the term “trans” represents substituents on opposite sides of the plane of the ring. Mixtures of compounds wherein the substituents are disposed on both the same and opposite sides of plane of the ring are designated “cis/trans.”
Certain compounds described herein may exist as a single tautomer or as a mixture of tautomers. For example, certain guanidine compounds having a hydrogen atom attached to at least one of the guanidine nitrogen atoms can exist as a single tautomer or a mixture of tautomers. To illustrate, depending upon the substituents attached at the R1, R2 and R3 positions, the guanidine compound may exist as a single tautomer represented by A, B, or C, or as mixture of two or more of A, B, and C.




The compounds disclosed herein can exist in solvated as well as unsolvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms.
The invention also embraces isotopically labeled compounds of the invention which are identical to those recited herein, except that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as 2H, 3H, 13C, 14C, 15N, 18O, 17O, 31P, 32P, 35S, 18F, and 36Cl, Respectively.
Certain isotopically-labeled disclosed compounds (e.g., those labeled with 3H and 14C) are useful in compound and/or substrate tissue distribution assays. Tritiated (i.e., 3H) and carbon-14 (i.e., 14C) isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (i.e., 2H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labeled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the e.g., Examples herein by substituting an isotopically labeled reagent for a non-isotopically labeled reagent.
The term “IC50” is art-recognized and refers to the concentration of a compound that is required for 50% inhibition of its target.
The term “EC50” is art-recognized and refers to the concentration of a compound at which 50% of its maximal effect is observed.
The terms “subject” and “patient” refer to organisms to be treated by the methods of the present invention. Such organisms preferably include, but are not limited to, mammals (e.g., murines, simians, equines, bovines, porcines, canines, felines, and the like), and most preferably includes humans. In the context of the invention, the terms “subject” and “patient” generally refer to an individual who will receive or who has received treatment (e.g., administration of a compound of the present invention and optionally one or more other agents) for a condition characterized by the dysregulation of apoptotic processes.
As used herein, the term “effective amount” refers to the amount of a compound sufficient to effect beneficial or desired results. An effective amount can be administered in one or more administrations, applications or dosages and is not intended to be limited to a particular formulation or administration route. As used herein, the term “treating” includes any effect, e.g., lessening, reducing, modulating, ameliorating or eliminating, that results in the improvement of the condition, disease, disorder, and the like, or ameliorating a symptom thereof.
The phrase “pathologically proliferating or growing cells” refers to a localized population of proliferating cells in an animal that is not governed by the usual limitations of normal growth.
As used herein, the term “un-activated target cell” refers to a cell that is either in the Go phase or one to which a stimulus has not been applied.
As used herein, the term “activated target lymphoid cell” refers to a lymphoid cell that has been primed with an appropriate stimulus to cause a signal transduction cascade, or alternatively, a lymphoid cell that is not in Go phase. Activated lymphoid cells may proliferate, undergo activation induced cell death, or produce one or more cytotoxins, cytokines, or other related membrane-associated proteins characteristic of the cell type (e.g., CD8+ or CD4+). They are also capable of recognizing and binding any target cell that displays a particular antigen on its surface, and subsequently releasing its effector molecules.
As used herein, the term “activated cancer cell” refers to a cancer cell that has been primed with an appropriate stimulus to cause signal transduction. An activated cancer cell may or may not be in the GO phase.
An activating agent is a stimulus that upon interaction with a target cell results in a signal transduction cascade. Examples of activating stimuli include, but are not limited to, small molecules, radiant energy, and molecules that bind to cell activation cell surface receptors. Responses induced by activation stimuli can be characterized by changes in, among others, intracellular Ca2+, superoxide, or hydroxyl radical levels; the activity of enzymes like kinases or phosphatases; or the energy state of the cell. For cancer cells, activating agents also include transforming oncogenes.
As used herein, the term “dysregulation of the process of cell death” refers to any aberration in the ability (e.g., predisposition) of a cell to undergo cell death via either necrosis or apoptosis. Dysregulation of cell death is associated with or induced by a variety of conditions, including for example, immune disorders (e.g., systemic lupus erythematosus, autoimmune disorders, rheumatoid arthritis, graft-versus-host disease, myasthenia gravis, Sjögren's syndrome, etc.), chronic inflammatory conditions (e.g., psoriasis, asthma and Crohn's disease), hyperproliferative disorders (e.g., tumors, B cell lymphomas, T cell lymphomas, etc.), viral infections (e.g., herpes, papilloma, HIV), and other conditions such as osteoarthritis and atherosclerosis.
It should be noted that when the dysregulation is induced by or associated with a viral infection, the viral infection may or may not be detectable at the time dysregulation occurs or is observed. That is, viral-induced dysregulation can occur even after the disappearance of symptoms of viral infection.
A “hyperproliferative disorder,” as used herein refers to any condition in which a localized population of proliferating cells in an animal is not governed by the usual limitations of normal growth. Examples of hyperproliferative disorders include tumors, neoplasms, lymphomas and the like. A neoplasm is said to be benign if it does not undergo invasion or metastasis and malignant if it does either of these. A metastatic cell or tissue means that the cell can invade and destroy neighboring body structures. Hyperplasia is a form of cell proliferation involving an increase in cell number in a tissue or organ, without significant alteration in structure or function. Metaplasia is a form of controlled cell growth in which one type of fully differentiated cell substitutes for another type of differentiated cell. Metaplasia can occur in epithelial or connective tissue cells. A typical metaplasia involves a somewhat disorderly metaplastic epithelium.
The pathological growth of activated lymphoid cells often results in an immune disorder or a chronic inflammatory condition. As used herein, the term “immune disorder” refers to any condition in which an organism produces antibodies or immune cells which recognize the organism's own molecules, cells or tissues. Non-limiting examples of immune disorders include autoimmune disorders, immune hemolytic anemia, immune hepatitis, Berger's disease or IgA nephropathy, Celiac Sprue, chronic fatigue syndrome, Crohn's disease, dermatomyositis, fibromyalgia, graft-versus-host disease, Grave's disease, Hashimoto's thyroiditis, idiopathic thrombocytopenia purpura, lichen planus, multiple sclerosis, myasthenia gravis, psoriasis, rheumatic fever, rheumatic arthritis, scleroderma, Sjorgren syndrome, systemic lupus erythematosus, type 1 diabetes, ulcerative colitis, vitiligo, tuberculosis, and the like.
As used herein, the term “chronic inflammatory condition” refers to a condition wherein the organism's immune cells are activated. Such a condition is characterized by a persistent inflammatory response with pathologic sequelae. This state is characterized by infiltration of mononuclear cells, proliferation of fibroblasts and small blood vessels, increased connective tissue, and tissue destruction. Examples of chronic inflammatory diseases include, but are not limited to, Crohn's disease, psoriasis, chronic obstructive pulmonary disease, inflammatory bowel disease, multiple sclerosis, and asthma. Immune diseases such as rheumatoid arthritis and systemic lupus erythematosus can also result in a chronic inflammatory state.
As used herein, the term “co-administration” refers to the administration of at least two agent(s) (e.g., a compound of the present invention) or therapies to a subject. In some embodiments, the co-administration of two or more agents/therapies is concurrent. In other embodiments, a first agent/therapy is administered prior to a second agent/therapy. Those of skill in the art understand that the formulations and/or routes of administration of the various agents/therapies used may vary. The appropriate dosage for co-administration can be readily determined by one skilled in the art. In some embodiments, when agents/therapies are co-administered, the respective agents/therapies are administered at lower dosages than appropriate for their administration alone. Thus, co-administration is especially desirable in embodiments where the co-administration of the agents/therapies lowers the requisite dosage of a known potentially harmful (e.g., toxic) agent(s).
As used herein, the term “pharmaceutical composition” refers to the combination of an active agent with a carrier, inert or active, making the composition especially suitable for diagnostic or therapeutic use in vivo or ex vivo.
As used herein, the term “pharmaceutically acceptable carrier” refers to any of the standard pharmaceutical carriers, such as a phosphate buffered saline solution, water, emulsions (e.g., such as an oil/water or water/oil emulsions), and various types of wetting agents. The compositions also can include stabilizers and preservatives. For examples of carriers, stabilizers and adjuvants. (See e.g., Martin, Remington's Pharmaceutical Sciences, 15th Ed., Mack Publ. Co., Easton, Pa. [1975]).
As used herein, the term “pharmaceutically acceptable salt” refers to any pharmaceutically acceptable salt (e.g., acid or base) of a compound of the present invention which, upon administration to a subject, is capable of providing a compound of this invention or an active metabolite or residue thereof. As is known to those of skill in the art, “salts” of the compounds of the present invention may be derived from inorganic or organic acids and bases. Examples of acids include, but are not limited to, hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene-p-sulfonic, tartaric, acetic, citric, methanesulfonic, ethanesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic, benzenesulfonic acid, and the like. Other acids, such as oxalic, while not in themselves pharmaceutically acceptable, may be employed in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable acid addition salts.
Examples of bases include, but are not limited to, alkali metals (e.g., sodium) hydroxides, alkaline earth metals (e.g., magnesium), hydroxides, ammonia, and compounds of formula NW4+, wherein W is C1-4 alkyl, and the like.
Examples of salts include, but are not limited to: acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, flucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, palmoate, pectinate, persulfate, phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, tosylate, undecanoate, and the like. Other examples of salts include anions of the compounds of the present invention compounded with a suitable cation such as Na+, NH4+, and NW4+ (wherein W is a C1-4 alkyl group), and the like.
For therapeutic use, salts of the compounds of the present invention are contemplated as being pharmaceutically acceptable. However, salts of acids and bases that are non-pharmaceutically acceptable may also find use, for example, in the preparation or purification of a pharmaceutically acceptable compound.
As used herein, the term “modulate” refers to the activity of a compound (e.g., a compound of the present invention) to affect (e.g., to promote or retard) an aspect of cellular function, including, but not limited to, cell growth, proliferation, apoptosis, and the like.
Throughout the description, where compositions are described as having, including, or comprising specific components, or where processes and methods are described as having, including, or comprising specific steps, it is contemplated that, additionally, there are compositions of the present invention that consist essentially of, or consist of, the recited components, and that there are processes and methods according to the present invention that consist essentially of, or consist of, the recited processing steps.
As a general matter, compositions specifying a percentage are by weight unless otherwise specified. Further, if a variable is not accompanied by a definition, then the previous definition of the variable controls.
I. Modulators of F1F0-ATPase Activity
In some embodiments, the present invention regulates F1F0-ATPase activity (e.g., mitochondrial F1F0-ATPase activity) through the exposure of cells to compounds of the present invention. In some embodiments, the compounds inhibit ATP synthesis and ATP hydrolysis. The effect of the compounds can be measured by detecting any number of cellular changes. For example, mitochondrial F1F0-ATPase activity and/or cell death may be assayed as described herein and in the art. In some embodiments, cell lines are maintained under appropriate cell culturing conditions (e.g., gas (CO2), temperature and media) for an appropriate period of time to attain exponential proliferation without density dependent constraints. Cell number and or viability are measured using standard techniques, such as trypan blue exclusion/hemo-cytometry, or an Alamar Blue or MTT dye conversion assay. Alternatively, the cell may be analyzed for the expression of genes or gene products associated with aberrations in apoptosis or necrosis.
In some embodiments, exposing the compounds of the present invention to a cell induces apoptosis. In some embodiments, the present invention induces apoptosis or arrest of cell proliferation through interacting with the mitochondrial F1F0-ATPase. In some embodiments, the compounds of the present invention inhibit mitochondrial F1F0-ATPase activity through binding the OSCP. In some embodiments, the compounds of the present invention bind the junction between the OSCP and the F1 subunit of the mitochondrial F1F0-ATPase. In some embodiments, the compounds of the present invention bind the F1 subunit. In certain embodiments, screening assays of the present invention permit detection of binding partners of the OSCP, F1, or OSCP/F1 junction.
In some embodiments, exposing a compound of the present invention to a cell induces apoptosis. In some embodiments, the present invention causes an initial increase in cellular ROS levels (e.g., O2−). In further embodiments, exposure of the compounds of the present invention to a cell causes an increase in cellular O2− levels. In still further embodiments, the increase in cellular O2− levels resulting from the compounds of the present invention is detectable with a redox-sensitive agent that reacts specifically with O2− (e.g., dihydroethidium (DHE)).
In some embodiments, the present invention causes a collapse of a cell's mitochondrial transmembrane potential (ΔΨm). In some embodiments, a collapse of a cell's mitochondrial ΔΨm resulting from the present invention is detectable with a mitochondria-selective potentiometric probe (e.g., 3,3′-Dihexyloxacarbocyanine iodide, DiOC6). In further embodiments, a collapse of a cell's mitochondrial ΔΨm resulting from the present invention occurs after an initial increase in cellular O2− levels.
In some embodiments, the present invention enables caspase activation. In other embodiments, the present invention causes the release of cytochrome c from mitochondria. In further embodiments, the present invention alters cystolic cytochrome c levels. In still other embodiments, altered cystolic cytochrome c levels resulting from the present invention are detectable by immunoblotting cytosolic fractions. In some embodiments, diminished cystolic cytochrome c levels resulting from the present invention are detectable after a period of time (e.g., 10 hours). In further preferred embodiments, diminished cystolic cytochrome c levels resulting from the present invention are detectable after 5 hours.
In other embodiments, the present invention causes the opening of the mitochondrial permeability transition pore. In some embodiments, the cellular release of cytochrome c resulting from the present invention is consistent with a collapse of mitochondrial ΔΨm. In still further preferred embodiments, the present invention causes an increase in cellular O2− levels after a mitochondrial ΔΨm collapse and a release of cytochrome c. In further preferred embodiments, a rise in cellular O2− levels is caused by a mitochondrial ΔΨm collapse and release of cytochrome c resulting from the present invention.
In other embodiments, the present invention causes cellular caspase activation. In some embodiments, caspase activation resulting from the present invention is measurable with a pan-caspase sensitive fluorescent substrate (e.g., FAM-VAD-fmk). In still further embodiments, caspase activation resulting from the present invention tracks with a collapse of mitochondrial ΔΨm. In other embodiments, the present invention causes an appearance of hypodiploid DNA. In some embodiments, an appearance of hypodiploid DNA resulting from the present invention is slightly delayed with respect to caspase activation.
In some embodiments, the molecular target for the present invention is found within mitochondria. In further embodiments, the molecular target of the present invention involves the mitochondrial ATPase. The primary sources of cellular ROS include redox enzymes and the mitochondrial respiratory chain (hereinafter MRC). In some embodiments, cytochrome c oxidase (complex IV of the MRC) inhibitors (e.g., NaN3) preclude a present invention dependent increase in cellular ROS levels. In other preferred embodiments, the ubiquinol-cytochrome c reductase component of MRC complex III inhibitors (e.g., FK506) preclude a present invention dependent increase in ROS levels.
II. Pyrazolyl Guanidine Compounds
One aspect of the invention provides a family of compounds represented by Formula I:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
A1 is phenylene or a six-membered heteroarylene;
A2 is




R1 represents independently for each occurrence halogen, haloalkyl, alkyl, cycloalkyl, heterocycloalkyl, hydroxyl, C1-C6alkoxy, cyano, —CO2R8, —C(O)R9, —S(O)R9, —SO2R9, —SO2N(R10)(R11)C(O)N(R10)(R11), N(R10)(R11), or —N(R8)C(O)(R9);
R2 is hydrogen or alkyl;
R3 is aryl, aralkyl, cycloalkyl, —(C(R8)2)m-cycloalkyl, heteroaryl, heteroaralkyl, heterocycloalkyl, —(C(R8)2)m-heterocycloalkyl, alkyl, haloalkyl, hydroxyalkyl, —(C(R8)2)m-alkoxyl, —(C(R8)2)m—O—(C(R8)2)m-alkoxyl, or —(C(R8)2)m—CN, wherein said aryl, aralkyl, cycloalkyl, heteroaryl, heteroaralkyl, and heterocycloalkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, hydroxyalkyl, C1-C6alkoxy, cyano, and —C1-C6alkylene-CO2R8;
R4 is hydrogen, alkyl, or —C(O)R9; or R3 and R4 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
R5 is hydrogen or alkyl;
R6 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, heteroaralkyl, —CO2R8, or —C(O)N(R10)(R11), wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, hydroxyalkyl, C1-C6alkoxy, —O-aralkyl, and cyano;
R7 is hydrogen, halogen, alkyl, or haloalkyl;
R8 represents independently for each occurrence hydrogen, alkyl, or cycloalkyl; or two occurrences of R8 attached to the same carbon atom are taken together with said carbon atom to form a saturated carbocylic ring;
R9 represents independently for each occurrence alkyl or cycloalkyl;
R10 and R11 each represent independently for each occurrence hydrogen, alkyl, or cycloalkyl; or R10 and R11 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
n is 0, 1, 2, or 3; and
m is 1, 2, 3, 4, or 5.
Definitions of the variables in Formula I above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where A1 is phenylene, R1 is halogen or haloalkyl, R2 is hydrogen, and R4 is hydrogen.
In certain embodiments, A1 is phenylene. In certain embodiments, A1 is a six-membered heteroarylene, such as pyridinylene or pyrimidinylene. In certain embodiments, A1 is pyridinylene.
In certain embodiments, A2 is




In certain embodiments, A2 is





In certain embodiments, R1 is halogen or haloalkyl. In certain embodiments, R1 is chloro, fluoro, or trifluoromethyl.
In certain embodiments, R2 is hydrogen. In certain embodiments, R2 is alkyl, such as methyl or ethyl. In certain embodiments, R4 is hydrogen. In certain embodiments, R4 is alkyl, such as methyl or ethyl. In certain embodiments, R2 and R4 are hydrogen. In certain embodiments, R4 is —C(O)R7. In certain embodiments, R2 and R4 are hydrogen.
In certain embodiments, R3 is alkyl or cycloalkyl. In certain embodiments, R3 is —(C(R8)2)m-alkoxyl. In certain embodiments, R3 is aryl or aralkyl, each of which is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is phenyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is phenyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.
In certain embodiments, R3 is benzyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is benzyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl. In certain embodiments, R3 is benzyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl.
In certain embodiments, R3 is alkyl, hydroxyalkyl, or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and hydroxyl. In certain embodiments, R3 is alkyl or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, and alkyl. In certain embodiments, R3 is heteroaryl or heteroaralkyl, wherein said heteroaryl and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is haloalkyl.
In certain embodiments, R5 is hydrogen. In certain embodiments, R5 is methyl.
In certain embodiments, R6 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, and alkyl. In certain embodiments, R6 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R6 is haloalkyl. In certain embodiments, R6 is trifluoromethyl. In certain embodiments, R6 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and hydroxyl. In certain embodiments, R6 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro and fluoro.
In certain embodiments, R7 is hydrogen. In certain embodiments, R7 is trifluoromethyl.
In certain embodiments, R8 represents independently for each occurrence hydrogen or methyl. In certain embodiments, R8 is hydrogen.
In certain embodiments, R9 is alkyl, such as methyl or ethyl.
In certain embodiments, R10 represents independently for each occurrence hydrogen or alkyl. In certain embodiments, R11 represents independently for each occurrence hydrogen or alkyl.
In certain embodiments, n is 1 or 2. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, m is 1 or 2.
Another aspect of the invention provides a family of compounds represented by Formula I-A:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
A1 is phenylene or a six-membered heteroarylene;
A2 is




R1 represents independently for each occurrence halogen, alkyl, haloalkyl, hydroxyl, C1-C6alkoxy, or cyano;
R2 is hydrogen or alkyl;
R3 is aryl, aralkyl, cycloalkyl, —(C(R8)2)m-cycloalkyl, heteroaryl, heteroaralkyl, heterocycloalkyl, —(C(R8)2)m-heterocycloalkyl, alkyl, haloalkyl, hydroxyalkyl, —(C(R8)2)m-alkoxyl, or —(C(R8)2)m—CN, wherein said aryl, aralkyl, cycloalkyl, heteroaryl, heteroaralkyl, and heterocycloalkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, C1-C6alkoxy, and cyano;
R4 is hydrogen, alkyl, or —C(O)R9; or R3 and R4 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
R5 is hydrogen or alkyl;
R6 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, or heteroaralkyl, wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, C1-C6alkoxy, and cyano;
R7 is hydrogen, alkyl, or haloalkyl;
R8 represents independently for each occurrence hydrogen, alkyl, or cycloalkyl;
R9 represents independently for each occurrence alkyl or cycloalkyl;
n is 0, 1, 2, or 3; and
m is 1, 2, 3, 4, or 5.
Definitions of the variables in Formula I-A above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where A1 is phenylene, R1 is halogen or haloalkyl, R2 is hydrogen, and R4 is hydrogen.
In certain embodiments, the compound is a compound of Formula I-A wherein A1 is phenylene. In certain embodiments, A1 is a six-membered heteroarylene, such as pyridinylene or pyrimidinylene. In certain embodiments, A1 is pyridinylene.




In certain embodiments, A2 is




In certain embodiments, A2 is

In certain embodiments, R1 is halogen or haloalkyl. In certain embodiments, R1 is chloro, fluoro, or trifluoromethyl.
In certain embodiments, R2 is hydrogen. In certain embodiments, R2 is alkyl, such as methyl or ethyl. In certain embodiments, R4 is hydrogen. In certain embodiments, R4 is alkyl, such as methyl or ethyl. In certain embodiments, R2 and R4 are hydrogen. In certain embodiments, R4 is —C(O)R7. In certain embodiments, R2 and R4 are hydrogen.
In certain embodiments, R3 is aryl or aralkyl, each of which is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is phenyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is phenyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.
In certain embodiments, R3 is benzyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl. In certain embodiments, R3 is benzyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl. In certain embodiments, R3 is benzyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl.
In certain embodiments, R3 is alkyl, hydroxyalkyl, or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and hydroxyl. In certain embodiments, R3 is alkyl or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, and alkyl. In certain embodiments, R3 is heteroaryl or heteroaralkyl, wherein said heteroaryl and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is haloalkyl.
In certain embodiments, R5 is hydrogen. In certain embodiments, R5 is methyl.
In certain embodiments, R6 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R6 is haloalkyl. In certain embodiments, R6 is trifluoromethyl. In certain embodiments, R6 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro and fluoro.
In certain embodiments, R7 is hydrogen. In certain embodiments, R7 is trifluoromethyl.
In certain embodiments, R8 represents independently for each occurrence hydrogen or methyl. In certain embodiments, R8 is hydrogen.
In certain embodiments, R9 is alkyl, such as methyl or ethyl.
In certain embodiments, n is 1 or 2. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, m is 1 or 2.
Another aspect of the invention provides a family of compounds represented by Formula I-A1:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
R1 and R2 each represent independently for each occurrence hydrogen, chloro, fluoro, or —CF3;
R3 is alkyl, cycloalkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, or C1-C6alkylene-C1-C6alkoxy, wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, C1-C6alkoxy, and cyano; and
R4 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, or heteroaralkyl, wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, C1-C6alkoxy, and cyano.
Definitions of the variables in Formula I-A1 above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where R1 is chloro or fluoro; R3 is aryl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl; and R4 is haloalkyl.
Accordingly, in certain embodiments, R1 and R2 are chloro or fluoro.
In certain embodiments, R3 is alkyl or cycloalkyl. In certain embodiments, R3 is C1-C6alkylene-C1-C6alkoxy. In certain embodiments, R3 is aryl, aralkyl, heteroaryl, heteroaralkyl, or C1-C6alkylene-C1-C6alkoxy, wherein said aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, C1-C6alkoxy, and cyano. In certain embodiments, R3 is aryl or aralkyl, each of which is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is phenyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is phenyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.
In certain embodiments, R3 is benzyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is benzyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl. In certain embodiments, R3 is benzyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is alkyl or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl.
In certain embodiments, R3 is heteroaryl or heteroaralkyl, wherein said heteroaryl and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is haloalkyl.
In certain embodiments, R4 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R4 is haloalkyl. In certain embodiments, R4 is trifluoromethyl. In certain embodiments, R4 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and hydroxyl. In certain embodiments, R4 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro and fluoro.
Another aspect of the invention provides a family of compounds represented by Formula I-B:




or a pharmaceutically acceptable salt thereof; wherein:
R1 and R2 each represent independently for each occurrence hydrogen, chloro, fluoro, or —CF3;
R3 is aryl, aralkyl, heteroaryl, or heteroaralkyl, wherein said aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl; and
R4 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, or heteroaralkyl, wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl.
Definitions of the variables in Formulae I-B above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where R1 is chloro or fluoro; R3 is aryl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl; and R4 is haloalkyl.
Accordingly, in certain embodiments, R1 and R2 are chloro or fluoro.
In certain embodiments, R3 is aryl or aralkyl, each of which is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is phenyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is phenyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.
In certain embodiments, R3 is benzyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is benzyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl. In certain embodiments, R3 is benzyl substituted with 1, 2, or 3 substituents independently selected from the group consisting of chloro, fluoro, trifluoromethyl, cyclopropyl, and (C1-C4)alkyl. In certain embodiments, R3 is alkyl or cycloalkyl, wherein said cycloalkyl is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl.
In certain embodiments, R3 is heteroaryl or heteroaralkyl, wherein said heteroaryl and heteroaralkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R3 is haloalkyl.
In certain embodiments, R4 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R4 is haloalkyl. In certain embodiments, R4 is trifluoromethyl. In certain embodiments, R4 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro and fluoro.
Another aspect of the invention provides a family of compounds represented by Formula II:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
A1 is phenylene or a six-membered heteroarylene;
X is —[C(R5)2]m or —[C(R5)2]p—C(O)—[C(R5)2]p;
R1 represents independently for each occurrence halogen, haloalkyl, alkyl, cycloalkyl, heterocycloalkyl, hydroxyl, C1-C6alkoxy, cyano, —CO2R6, —C(O)R7, —S(O)R7, —SO2R7, —SO2N(R8)(R9), —C(O)N(R8)(R9), —N(R8)(R9), or —N(R6)C(O)(R7);
R2 is hydrogen or alkyl;
R3 is hydrogen, aryl, aralkyl, cycloalkyl, —(C(R6)2)m-cycloalkyl, heteroaryl, heteroaralkyl, heterocycloalkyl, —(C(R6)2)m-heterocycloalkyl, alkyl, haloalkyl, hydroxyalkyl, —(C(R6)2)m-alkoxyl, —(C(R6)2)m—O—(C(R6)2)m-alkoxyl, or —(C(R6)2)m—CN, wherein said aryl, aralkyl, cycloalkyl, heteroaryl, heteroaralkyl, and heterocycloalkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, hydroxyalkyl, C1-C6alkoxy, cyano, and —C1-C6alkylene-CO2R6; or R3 and a single occurrence of R5 are taken together with the atoms to which they are attached to form a saturated heterocyclic ring;
R4 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, heteroaralkyl, —CO2R6, or —C(O)N(R8)(R9), wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, hydroxyalkyl, C1-C6alkoxy, —O-aralkyl, and cyano;
R5 represents independently for each occurrence hydrogen or alkyl; or R3 and a single occurrence of R5 are taken together with the atoms to which they are attached to form a saturated heterocyclic ring;
R6 represents independently for each occurrence hydrogen, alkyl, or cycloalkyl; or two occurrences of R6 attached to the same carbon atom are taken together with said carbon atom to form a saturated carbocylic ring;
R7 represents independently for each occurrence alkyl or cycloalkyl;
R8 and R9 each represent independently for each occurrence hydrogen, alkyl, or cycloalkyl; or R8 and R9 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
n is 0, 1, 2, or 3;
m represents independently for each occurrence 1, 2, 3, 4, or 5; and
p represents independently for each occurrence 0, 1, or 2.
Definitions of the variables in Formulae II above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where R1 is halogen or haloalkyl; R3 is aryl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl; and R4 is haloalkyl.
Accordingly, in certain embodiments, A1 is phenylene.
In certain embodiments, X is —[C(R5)2]m. In certain other embodiments, X is —C(O)—.
In certain embodiments, R1 is halogen.
In certain embodiments, R3 is hydrogen or aralkyl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, and alkyl.
In certain embodiments, R4 is alkyl, haloalkyl, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl. In certain embodiments, R4 is trifluoromethyl. In certain embodiments, R4 is phenyl optionally substituted with 1 or 2 substituents independently selected from the group consisting of chloro and fluoro.
In certain embodiments, R5 is hydrogen.
In certain embodiments, n is 1 or 2.
In certain embodiments, m is 0.
Another aspect of the invention provides a family of compounds represented by Formula II-A:




including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
A1 is phenylene or a six-membered heteroarylene;
X is —[C(R5)2]m or —[C(R5)2]p—C(O)—[C(R5)2]p;
R1 represents independently for each occurrence halogen, haloalkyl, alkyl, cycloalkyl, heterocycloalkyl, hydroxyl, C1-C6alkoxy, cyano, —CO2R6, —C(O)R7, —SO2R7, —SO2N(R8)(R9), —C(O)N(R8)(R9), —N(R8)(R9), or —N(R6)C(O)(R7);
R2 is hydrogen or alkyl;
R3 is hydrogen, aryl, aralkyl, cycloalkyl, —(C(R6)2)m-cycloalkyl, heteroaryl, heteroaralkyl, heterocycloalkyl, —(C(R6)2)m-heterocycloalkyl, alkyl, haloalkyl, hydroxyalkyl, —(C(R6)2)m-alkoxyl, —(C(R6)2)m—O—(C(R6)2)m-alkoxyl, or —(C(R6)2)m—CN, wherein said aryl, aralkyl, cycloalkyl, heteroaryl, heteroaralkyl, and heterocycloalkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, hydroxyalkyl, C1-C6alkoxy, cyano, and —C1-C6alkylene-CO2R6; or R3 and a single occurrence of R5 are taken together with the atoms to which they are attached to form a saturated heterocyclic ring;
R4 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, heteroaralkyl, —CO2R6, or —C(O)N(R8)(R9), wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, hydroxyalkyl, C1-C6alkoxy, and cyano;
R5 represents independently for each occurrence hydrogen or alkyl; or R3 and a single occurrence of R5 are taken together with the atoms to which they are attached to form a saturated heterocyclic ring;
R6 represents independently for each occurrence hydrogen, alkyl, or cycloalkyl; or two occurrences of R6 attached to the same carbon atom are taken together with said carbon atom to form a saturated carbocylic ring;
R7 represents independently for each occurrence alkyl or cycloalkyl;
R8 and R9 each represent independently for each occurrence hydrogen, alkyl, or cycloalkyl; or R8 and R9 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
n is 0, 1, 2, or 3;
m represents independently for each occurrence 1, 2, 3, 4, or 5; and
p represents independently for each occurrence 0, 1, or 2.
Definitions of the variables in Formulae II-A above encompass multiple chemical groups. The application contemplates embodiments where, for example, i) the definition of a variable is a single chemical group selected from those chemical groups set forth above, ii) the definition is a collection of two or more of the chemical groups selected from those set forth above, and iii) the compound is defined by a combination of variables in which the variables are defined by (i) or (ii), e.g., such as where R1 is halogen or haloalkyl; R3 is aryl optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, and alkyl; and R4 is haloalkyl. In certain other embodiments, variables A1, X, R1 through R9, n, m, and/or p for Formula II-A are as defined by one of the further embodiments specified above in connection with Formula II.
Further, the description above describes multiple embodiments providing definitions for variables used herein. The application specifically contemplates all combinations of such embodiments. For example, the application contemplates particular combinations of embodiments relating to Formula I-A, such as where A1 is phenylene, R1 is halogen or haloalkyl, and n is 1. Further, for example, the application contemplates particular combinations of embodiments relating to Formula I-A1, such as where R1 and R2 are independently chloro or fluoro, and R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.
In certain embodiments, the compound is one of the compounds listed in any one of Tables 1-4 below, or a pharmaceutically acceptable salt thereof. In certain other embodiments, the compound is one of the compounds listed in Table 1 below, or a pharmaceutically acceptable salt thereof. It is understood that the foregoing compounds can be combined with a pharmaceutically acceptable carrier to produce a pharmaceutical composition.







TABLE 1













 











No.
A
X
Y
Z





I-1 
3-chlorophenyl




H
3-chlorophenyl


 


I-2 
4-chlorophenyl




H
4-chlorophenyl


 


I-3 
3-fluorophenyl




H
3,5-dichlorophenyl


 


I-4 
4-fluorophenyl




H
3-chloro-4-fluorophenyl


 


I-5 
3,4-dichlorophenyl




H
3-chloro-5-fluorophenyl


 


I-6 
3,4-difluorophenyl




H
3-trifluoromethylphenyl


 


I-7 
4-trifluoromethylphenyl




H
3-cyclopropylphenyl


 


I-8 
3-chlorophenyl




H
3-tert-butylphenyl


 


I-9 
4-chlorophenyl




H
2-cyclopropylphenyl


 


I-10
3-fluorophenyl




H
2-cyclopropyl-4- fluorophenyl


 


I-11
4-fluorophenyl




H
3-chlorobenzyl


 


I-12
3,4-dichlorophenyl




H
4-chlorobenzyl


 


I-13
3,4-difluorophenyl




H
3-fluorobenzyl


 


I-14
4-trifluoromethylphenyl




H
4-fluorobenzyl


 


I-15
3-chlorophenyl




H
3-chloro-5-fluorobenzyl


 


I-16
4-chlorophenyl




H
3,5-dichlorobenzyl


 


I-17
3-fluorophenyl




H
3,5-difluorobenzyl


 


I-18
4-fluorophenyl




H
3-cyclopropylbenzyl


 


I-19
3,4-dichlorophenyl




H
3-trifluoromethylbenzyl


 


I-20
3,4-difluorophenyl




H
4-trifluoromethylbenzyl


 


I-21
4-trifluoromethylphenyl




H
cyclopropyl


 


I-22
3-chlorophenyl




H
cyclopentyl


 


I-23
4-chlorophenyl




H
cyclohexyl


 


I-24
3-fluorophenyl




H
4-methylcyclohexyl


 


I-25
4-fluorophenyl




H
ethyl


 


I-26
3,4-dichlorophenyl




H
tert-butyl


 


I-27
3,4-difluorophenyl




H
2,2,2-trifluoroethyl


 


I-28
4-trifluoromethylphenyl




H
1-methylcyclobutyl


 


I-29
3-chlorophenyl




H
3-chloro-4-fluorophenyl


 


I-30
4-chlorophenyl




H
3-chloro-5-fluorophenyl


 


I-31
3-fluorophenyl




H
3-chlorophenyl


 


I-32
4-fluorophenyl




H
4-chlorophenyl


 


I-33
3,4-dichlorophenyl




H
3,5-dichlorophenyl


 


I-34
3,4-difluorophenyl




H
3-chloro-4-fluorophenyl


 


I-35
4-trifluoromethylphenyl




H
3-chloro-5-fluorophenyl


 


I-36
3-chlorophenyl




H
3-trifluoromethylphenyl


 


I-37
4-chlorophenyl




H
3-chlorophenyl


 


I-38
3-fluorophenyl




H
4-chlorophenyl


 


I-39
4-fluorophenyl




H
3,5-dichlorophenyl


 


I-40
3-chlorophenyl




—C(O)Me
3-chlorophenyl


 


I-41
4-chlorophenyl




—C(O)Me
4-chlorophenyl


 


I-42
3-trifluorophenyl




—C(O)Me
3-chloro-4-fluorophenyl


 


I-43
3-fluorophenyl




—CH3
3-chlorophenyl


 


I-44
4-fluorophenyl




—CH3
4-chlorophenyl


 


I-45
3-trifluorophenyl




—CH3
3-chloro-4-fluorophenyl


 


I-46








H
4-chlorophenyl


 


I-47








H
3-chloro-4-fluorophenyl


 


I-48








H
4-chlorophenyl


 


I-49








H
3-chloro-4-fluorophenyl


 


I-50








H
4-chlorophenyl


 


I-51








H
3-chloro-4-fluorophenyl


 


I-52








H
4-chlorophenyl


 


I-53








H
3-chloro-4-fluorophenyl


 


I-54








H
4-chlorophenyl


 


I-55








H
3-chloro-4-fluorophenyl


 


I-56
4-trifluoromethylphenyl




H
tert-butyl


 


I-57








H
tert-butyl


 


I-58








H
tert-butyl


 


I-59








H






 


I-60








H
tert-butyl









In certain other embodiments, the compound is one of the compounds listed in Examples 1-18, or a pharmaceutically acceptable salt of said compounds. It is understood that the foregoing compounds can be combined with a pharmaceutically acceptable carrier to produce a pharmaceutical composition.
Exemplary methods for preparing compounds described herein are provided in the examples. Further exemplary procedures for making various compounds described herein are described in Scheme 1 below. The synthetic scheme is provided for the purpose of illustrating the invention, but not for limiting the scope or spirit of the invention. Starting materials can be obtained from commercial sources or be prepared based on procedures described in the literature.
The synthetic route in Scheme 1 involves reacting an optionally substituted benzoylchloride with potassium thiocyanate to form an acyl isothiocyanate intermediate. This acyl isothiocyanate intermediate is treated with an amino-pyrazole compound to form an acyl thiourea. The acyl thiourea is reacted with 1-ethyl-2′,2′-dimethylaminopropylcarbodiimide (EDCI) and a second amine compound (e.g., an aniline or benzylamine) to form the desired pyrazolyl guanidine compound. To the extent either the amino-pyrazole or the second amine compound contain a further functional group that may undergo reaction under the conditions illustrated in Scheme 1, standard protecting group strategies for protection and deprotection may be employed. See, for example, Greene, T. W.; Wuts, P. G. M. Protective Groups in Organic Synthesis, 2nd ed.; Wiley: New York, 1991. It understood that the optionally substituted benzoylchloride starting material can be replaced with a heteroaryl acid chloride (i.e., nicotinoyl chloride) to prepare pyrazolyl guanidines containing a —C(O)-heteroaryl moiety.




III. Therapeutic Applications of Pyrazolyl Guanidine Compounds

It is contemplated that the guanidine compounds described herein, such as the guanidine compounds of Formula I, I-A, I-A1, I-B, II and II-A, provide therapeutic benefits to patients suffering from any one or more of a number of conditions, e.g., diseases characterized by dysregulation of F1F0-ATPase activity, diseases characterized by dysregulation of necrosis and/or apoptosis processes in a cell or tissue, disease characterized by aberrant cell growth and/or hyperproliferation. The compounds described herein can also be used to treat a variety of dysregulatory disorders related to cellular death as described elsewhere herein. Additionally, the compounds described herein can be used to inhibit ATP synthesis.
Accordingly, one aspect of the invention provides a method of treating a subject suffering from a medical disorder. The method comprises administering to the subject a therapeutically effective amount of one or more pyrazolyl guanidine compounds described herein, e.g., a compound of Formula I, I-A, I-A1, I-B, II, or II-A, as described in Section II above, in order to ameliorate a symptom of the disorder.
A large number of medical disorders can be treated using the guanidine compounds described herein. For example, the compounds described herein can be used to treat medical disorders characterized by dysregulation of necrosis and/or apoptosis processes in a cell or tissue, diseases characterized by aberrant cell growth and/or hyperproliferation, etc., or lupus, rheumatoid arthritis, psoriasis, graft-versus-host disease, Crohn's disease, inflammatory bowel disease, multiple sclerosis, cardiovascular disease, myeloma, lymphoma, cancer, and bacterial infection. In certain embodiments, the cancer is a solid tumor, leukemia, colon cancer, pancreatic cancer, breast cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, lung cancer, small cell lung cancer, non-small cell lung cancer, bladder cancer, stomach cancer, cervical cancer, testicular tumor, skin cancer, rectal cancer, thyroid cancer, kidney cancer, uterus cancer, espophagus cancer, liver cancer, an acoustic neuroma, oligodendroglioma, meningioma, melanoma, neuroblastoma, or retinoblastoma.
Although not wishing to be bound to a particular theory, it is believed that the compounds impart therapeutic benefit by modulating (e.g., inhibiting or promoting) the activity of the F1F0-ATPase complexes (e.g., mitochondrial F1F0-ATPase complexes) in affected cells or tissues. In some embodiments, the compositions of the present invention are used to treat immune/chronic inflammatory conditions (e.g., psoriasis, autoimmune disorders, organ-transplant rejection, and epidermal hyperplasia). In further embodiments, the compositions of the present invention are used in conjunction with stenosis therapy to treat compromised (e.g., occluded) vessels.
In certain embodiments, a composition comprising a guanidine compound is administered under conditions (e.g., timing, dose, co-administration with other agent, mode of administration, selection of subject, use of targeting agents, etc.) that maximize desired effects directed at the F1F0-ATPase.
In certain embodiments, the medical disorder is an immune disorder. In certain other embodiments, the medical disorder is an inflammatory disorder. In certain other embodiments, the medical disorder is an autoimmune disorder. In certain other embodiments, the medical disorder is rheumatoid arthritis, psoriasis, chronic graft-versus-host disease, acute graft-versus-host disease, Crohn's disease, inflammatory bowel disease, multiple sclerosis, systemic lupus erythematosus, Celiac Sprue, idiopathic thrombocytopenic thrombotic purpura, myasthenia gravis, Sjogren's syndrome, scleroderma, ulcerative colitis, asthma, uveitis, or epidermal hyperplasia.
In certain other embodiments, the medical disorder is cartilage inflammation, bone degradation, arthritis, juvenile arthritis, juvenile rheumatoid arthritis, pauciarticular juvenile rheumatoid arthritis, polyarticular juvenile rheumatoid arthritis, systemic onset juvenile rheumatoid arthritis, juvenile ankylosing spondylitis, juvenile enteropathic arthritis, juvenile reactive arthritis, juvenile Reter's Syndrome, SEA Syndrome, juvenile dermatomyositis, juvenile psoriatic arthritis, juvenile scleroderma, juvenile systemic lupus erythematosus, juvenile vasculitis, pauciarticular rheumatoid arthritis, polyarticular rheumatoid arthritis, systemic onset rheumatoid arthritis, ankylosing spondylitis, enteropathic arthritis, reactive arthritis, Reter's Syndrome, dermatomyositis, psoriatic arthritis, vasculitis, myolitis, polymyolitis, dermatomyolitis, osteoarthritis, polyarteritis nodossa, Wegener's granulomatosis, arteritis, polymyalgia rheumatica, sarcoidosis, sclerosis, primary biliary sclerosis, sclerosing cholangitis, dermatitis, atopic dermatitis, atherosclerosis, Still's disease, chronic obstructive pulmonary disease, Guillain-Barre disease, Type I diabetes mellitus, Graves' disease, Addison's disease, Raynaud's phenomenon, or autoimmune hepatitis. In certain embodiments, the psoriasis is plaque psoriasis, guttate psoriasis, inverse psoriasis, pustular psoriasis, or erythrodermic psoriasis.
In certain other embodiments, the medical disorder is Crohn's disease, inflammatory bowel disease, multiple sclerosis, graft-versus-host disease, lupus, rheumatoid arthritis, or psoriasis. In certain other embodiments, the medical disorder is cardiovascular disease, myeloma, lymphoma, or cancer. In certain other embodiments, the medical disorder is lupus, rheumatoid arthritis, psoriasis, graft-versus-host disease, myeloma, or lymphoma. In certain other embodiments, the medical disorder is cardiovascular disease or cancer. In certain other embodiments, the medical disorder is Crohn's disease, inflammatory bowel disease, or multiple sclerosis. In certain other embodiments, the medical disorder is graft-versus-host disease. In further embodiments, the medical disorder is a bacterial infection. In certain embodiments, the patient (or subject) is a human.
As indicated above, the guanidine compounds described herein can be used in the treatment of a bacterial infection. A variety of bacteria are contemplated to be susceptible to the guanidine compounds. Representative bacteria include Staphylococci species, e.g., S. aureus; Enterococci species, e.g., E. faecalis and E. faecium; Streptococci species, e.g., S. pyogenes and S. pneumoniae; Escherichia species, e.g., E. coli, including enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic and enteroaggregative E. coli strains; Haemophilus species, e.g., H. influenza; and Moraxella species, e.g., M. catarrhalis. Other examples include Mycobacteria species, e.g., M. tuberculosis, M. avian-intracellulare, M. kansasii, M. Bovis, M. africanum, M. genavense, M. leprae, M. xenopi, M. simiae, M. scrofulaceum, M. malmoense, M. celatum, M. abscessus, M. chelonae, M. szulgai, M. gordonae, M. haemophilum, M. fortuni and M. marinum; Corynebacteria species, e.g., C. diphtheriae; Vibrio species, e.g., V. cholerae; Campylobacter species, e.g., C. jejuni; Helicobacter species, e.g., H. pylori; Pseudomonas species, e.g., P. aeruginosa; Legionella species, e.g., L. pneumophila; Treponema species, e.g., T. pallidum; Borrelia species, e.g., B. burgdorferi; Listeria species, e.g., L. monocytogenes; Bacillus species, e.g., B. cereus; Bordatella species, e.g., B. pertussis; Clostridium species, e.g., C. perfringens, C. tetani, C. difficile and C. botulinum; Neisseria species, e.g., N. meningitidis and N. gonorrhoeae; Chlamydia species, e.g., C. psittaci, C. pneumoniae and C. trachomatis; Rickettsia species, e.g., R. rickettsii and R. prowazekii; Shigella species, e.g., S. sonnei; Salmonella species, e.g., S. typhimurium; Yersinia species, e.g., Y. enterocolitica and Y. pseudotuberculosis; Klebsiella species, e.g., K. pneumoniae; Mycoplasma species, e.g., M. pneumoniae; and Trypanosoma brucei. In certain embodiments, the guanidine compounds described herein are used to treat a subject suffering from a bacterial infection selected from the group consisting of S. aureus, E. faecalis, E. faecium, S. pyogenes, S. pneumonia, and P. aeruginosa. In certain embodiments, the guanidine compounds described herein are used to treat a subject suffering from a Trypanosoma brucei infection.
The antibacterial activity of the compounds described herein may be evaluated using standard assays known in the art, such as the microbroth dilution minimum inhibition concentration (MIC) assay, as further described in National Committee for Clinical Laboratory
Standards. Performance Standards for Antimicrobial Susceptibility Testing; Fourteenth Informational Supplement. NCCLS document M100-S14 {ISBN 1-56238-516-X}. This assay may be used to determine the minimum concentration of a compound necessary to prevent visible bacterial growth in a solution. In general, the drug to be tested is serially diluted into wells, and aliquots of liquid bacterial culture are added. This mixture is incubated under appropriate conditions, and then tested for growth of the bacteria. Compounds with low or no antibiotic activity (a high MIC) will allow growth at high concentrations of compound, while compounds with high antibiotic activity will allow bacterial growth only at lower concentrations (a low MIC).
The assay uses stock bacterial culture conditions appropriate for the chosen strain of bacteria. Stock cultures from the permanent stock culture collection can be stored as frozen suspensions at −70° C. Cultures may be suspended in 10% skim milk (BD) prior to snap freezing in dry ice/ethanol and then placed in a −70° C. freezer. Cultures may be maintained on Tryptic Soy Agar containing 5% Sheep Blood at room temperature (20° C.), and each culture may be recovered from frozen form and transferred an additional time before MIC testing. Fresh plates are inoculated the day before testing, incubated overnight, and checked to confirm purity and identity.
The identity and purity of the cultures recovered from the stock culture can be confirmed to rule out the possibility of contamination. The identity of the strains may be confirmed by standard microbiological methods (See, e.g., Murray et al., Manual of Clinical Microbiology, Eighth Edition. ASM Press {ISBN 1-55581-255-4}). In general, cultures are streaked onto appropriate agar plates for visualization of purity, expected colony morphology, and hemolytic patterns. Gram stains can also be utilized. The identities are confirmed using a MicroScan WalkAway 40 SI Instrument (Dade Behring, West Sacramento, Calif.). This device utilizes an automated incubator, reader, and computer to assess for identification purposes the biochemical reactions carried out by each organism. The MicroScan WalkAway can also be used to determine a preliminary MIC, which may be confirmed using the method described below.
Frozen stock cultures may be used as the initial source of organisms for performing microbroth dilution minimum inhibition concentration (MIC) testing. Stock cultures are passed on their standard growth medium for at least 1 growth cycle (18-24 hours) prior to their use. Most bacteria may be prepared directly from agar plates in 10 mL aliquots of the appropriate broth medium. Bacterial cultures are adjusted to the opacity of a 0.5 McFarland Standard (optical density value of 0.28-0.33 on a Perkin-Elmer Lambda EZ150 Spectrophotometer, Wellesley, Mass., set at a wavelength of 600 nm). The adjusted cultures are then diluted 400 fold (0.25 mL inoculum+100 mL broth) in growth media to produce a starting suspension of approximately 5×105 colony forming units (CFU)/mL. Most bacterial strains may be tested in cation adjusted Mueller Hinton Broth (CAMHB).
Test compounds (“drugs”) are solubilized in a solvent suitable for the assay, such as DMSO. Drug stock solutions may be prepared on the day of testing. Microbroth dilution stock plates may be prepared in two dilution series, 64 to 0.06 μg drug/mL and 0.25 to 0.00025 μg drug/mL. For the high concentration series, 200 μL of stock solution (2 mg/mL) is added to duplicate rows of a 96-well microtiter plate. This is used as the first well in the dilution series. Serial two-fold decremental dilutions are made using a BioMek FX robot (Beckman Coulter Inc., Fullerton, Calif.) with 10 of the remaining 11 wells, each of which will contain 100 μL of the appropriate solvent/diluent. Row 12 contains solvent/diluent only and serves as the control. For the first well of the low concentration series, 200 μL of an 8 μg/mL stock are added to duplicate rows of a 96-well plate. Serial two-fold dilutions are made as described above.
Daughter 96-well plates may be spotted (3.2 μL/well) from the stock plates listed above using the BioMek FX robot and used immediately or frozen at −70° C. until use. Aerobic organisms are inoculated (100 μL volumes) into the thawed plates using the BioMek FX robot. The inoculated plates are be placed in stacks and covered with an empty plate. These plates are then incubated for 16 to 24 hours in ambient atmosphere according to CLSI guidelines (National Committee for Clinical Laboratory Standards, Methods for Dilution, Antimicrobial Tests for Bacteria that Grow Aerobically; Approved Standard-Sixth Edition. NCCLS document M7-A6 {ISBN 1-56238-486-4}).
After inoculation and incubation, the degree of bacterial growth can be estimated visually with the aid of a Test Reading Mirror (Dynex Technologies 220 16) in a darkened room with a single light shining directly through the top of the microbroth tray. The MIC is the lowest concentration of drug that prevents macroscopically visible growth under the conditions of the test.
Additionally, any one or more of the pyrazolyl guanidine compounds described herein can be used to treat a F1F0-ATP hydrolase associated disorder (e.g., myocardial infarction, ventricular hypertrophy, coronary artery disease, non-Q wave MI, congestive heart failure, cardiac arrhythmias, unstable angina, chronic stable angina, Prinzmetal's angina, high blood pressure, intermittent claudication, peripheral occlusive arterial disease, thrombotic or thromboembolic symptoms of thromboembolic stroke, venous thrombosis, arterial thrombosis, cerebral thrombosis, pulmonary embolism, cerebral embolism, thrombophilia, disseminated intravascular coagulation, restenosis, atrial fibrillation, ventricular enlargement, atherosclerotic vascular disease, atherosclerotic plaque rupture, atherosclerotic plaque formation, transplant atherosclerosis, vascular remodeling atherosclerosis, cancer, surgery, inflammation, systematic infection, artificial surfaces, interventional cardiology, immobility, medication, pregnancy and fetal loss, and diabetic complications comprising retinopathy, nephropathy and neuropathy) in a subject.
Combination Therapy
Additionally, the guanidine compounds described herein can be used in combination with at least one other therapeutic agent, such as Bz-423 (a benzodiazepine compound as described in U.S. Pat. Nos. 7,144,880 and 7,125,866, U.S. patent application Ser. Nos. 11/586,097, 11/585,492, 11/445,010, 11/324,419, 11/176,719, 11/110,228, 10/935,333, 10/886,450, 10/795,535, 10/634,114, 10/427,211, 10/217,878, and 09/767,283, and U.S. Provisional Patent Nos. 60/878,519, 60/812,270, 60/802,394, 60/732,045, 60/730,711, 60/704,102, 60/686,348, 60/641,040, 60/607,599, and 60/565,788), potassium channel openers, calcium channel blockers, sodium hydrogen exchanger inhibitors, antiarrhythmic agents, antiatherosclerotic agents, anticoagulants, antithrombotic agents, prothrombolytic agents, fibrinogen antagonists, diuretics, antihypertensive agents, ATPase inhibitors, mineralocorticoid receptor antagonists, phospodiesterase inhibitors, antidiabetic agents, anti-inflammatory agents, antioxidants, angiogenesis modulators, antiosteoporosis agents, hormone replacement therapies, hormone receptor modulators, oral contraceptives, antiobesity agents, antidepressants, antianxiety agents, antipsychotic agents, antiproliferative agents, antitumor agents, antiulcer and gastroesophageal reflux disease agents, growth hormone agents and/or growth hormone secretagogues, thyroid mimetics, anti-infective agents, antiviral agents, antibacterial agents, antifungal agents, cholesterol/lipid lowering agents and lipid profile therapies, and agents that mimic ischemic preconditioning and/or myocardial stunning, antiatherosclerotic agents, anticoagulants, antithrombotic agents, antihypertensive agents, antidiabetic agents, and antihypertensive agents selected from ACE inhibitors, AT-1 receptor antagonists, ET receptor antagonists, dual ET/AII receptor antagonists, vasopepsidase inhibitors, an antiplatelet agent selected from GPIIb/IIIa blockers, P2Y1 and P2Y12 antagonists, thromboxane receptor antagonists, or aspirin, along with a pharmaceutically-acceptable carrier or diluent in a pharmaceutical composition.
IV. Pharmaceutical Compositions, Formulations, and Exemplary Administration Routes and Dosing Considerations
Exemplary embodiments of various contemplated medicaments and pharmaceutical compositions are provided below.
A. Preparing Medicaments
Compounds of the present invention are useful in the preparation of medicaments to treat a variety of conditions, such as conditions associated with dysregulation of cell death, aberrant cell growth and hyperproliferation. One of skill in the art will appreciate that any one or more of the compounds described herein, including the many specific embodiments, are prepared by applying standard pharmaceutical manufacturing procedures. Such medicaments can be delivered to the subject by using delivery methods that are well-known in the pharmaceutical arts.
B. Exemplary Pharmaceutical Compositions and Formulation
In some embodiments of the present invention, the compositions are administered alone, while in some other embodiments, the compositions are preferably present in a pharmaceutical formulation comprising at least one active ingredient/agent, as discussed above, together with a solid support or alternatively, together with one or more pharmaceutically acceptable carriers and optionally other therapeutic agents (e.g., those described in section III hereinabove). Each carrier should be “acceptable” in the sense that it is compatible with the other ingredients of the formulation and not injurious to the subject.
Contemplated formulations include those suitable for oral, rectal, nasal, topical (including transdermal, buccal and sublingual), vaginal, parenteral (including subcutaneous, intramuscular, intravenous and intradermal) and pulmonary administration. In some embodiments, formulations are conveniently presented in unit dosage form and are prepared by any method known in the art of pharmacy. Such methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing into association (e.g., mixing) the active ingredient with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets, wherein each preferably contains a predetermined amount of the active ingredient; as a powder or granules; as a solution or suspension in an aqueous or non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. In other embodiments, the active ingredient is presented as a bolus, electuary, or paste, etc.
In some embodiments, tablets comprise at least one active ingredient and optionally one or more accessory agents/carriers are made by compressing or molding the respective agents. In some embodiments, compressed tablets are prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder (e.g., povidone, gelatin, hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (e.g., sodium starch glycolate, cross-linked povidone, cross-linked sodium carboxymethyl cellulose) surface-active or dispersing agent. Molded tablets are made by molding in a suitable machine a mixture of the powdered compound (e.g., active ingredient) moistened with an inert liquid diluent. Tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile. Tablets may optionally be provided with an enteric coating, to provide release in parts of the gut other than the stomach.
Formulations suitable for topical administration in the mouth include lozenges comprising the active ingredient in a flavored basis, usually sucrose and acacia or tragacanth; pastilles comprising the active ingredient in an inert basis such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active ingredient in a suitable liquid carrier.
Pharmaceutical compositions for topical administration according to the present invention are optionally formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, sprays, aerosols or oils. In alternative embodiments, topical formulations comprise patches or dressings such as a bandage or adhesive plasters impregnated with active ingredient(s), and optionally one or more excipients or diluents. In some embodiments, the topical formulations include a compound(s) that enhances absorption or penetration of the active agent(s) through the skin or other affected areas. Examples of such dermal penetration enhancers include dimethylsulfoxide (DMSO) and related analogues.
If desired, the aqueous phase of a cream base includes, for example, at least about 30% w/w of a polyhydric alcohol, i.e., an alcohol having two or more hydroxyl groups such as propylene glycol, butane-1,3-diol, mannitol, sorbitol, glycerol and polyethylene glycol and mixtures thereof.
In some embodiments, oily phase emulsions of this invention are constituted from known ingredients in a known manner. This phase typically comprises a lone emulsifier (otherwise known as an emulgent), it is also desirable in some embodiments for this phase to further comprise a mixture of at least one emulsifier with a fat or an oil or with both a fat and an oil.
Preferably, a hydrophilic emulsifier is included together with a lipophilic emulsifier so as to act as a stabilizer. In some embodiments it is also preferable to include both an oil and a fat. Together, the emulsifier(s) with or without stabilizer(s) make up the so-called emulsifying wax, and the wax together with the oil and/or fat make up the so-called emulsifying ointment base which forms the oily dispersed phase of the cream formulations.
Emulgents and emulsion stabilizers suitable for use in the formulation of the present invention include Tween 60, Span 80, cetostearyl alcohol, myristyl alcohol, glyceryl monostearate and sodium lauryl sulfate.
The choice of suitable oils or fats for the formulation is based on achieving the desired properties (e.g., cosmetic properties), since the solubility of the active compound/agent in most oils likely to be used in pharmaceutical emulsion formulations is very low. Thus creams should preferably be non-greasy, non-staining and washable products with suitable consistency to avoid leakage from tubes or other containers. Straight or branched chain, mono- or dibasic alkyl esters such as di-isoadipate, isocetyl stearate, propylene glycol diester of coconut fatty acids, isopropyl myristate, decyl oleate, isopropyl palmitate, butyl stearate, 2-ethylhexyl palmitate or a blend of branched chain esters known as Crodamol CAP may be used, the last three being preferred esters. These may be used alone or in combination depending on the properties required. Alternatively, high melting point lipids such as white soft paraffin and/or liquid paraffin or other mineral oils can be used.
Formulations suitable for topical administration to the eye also include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent for the agent.
Formulations for rectal administration may be presented as a suppository with suitable base comprising, for example, cocoa butter or a salicylate.
Formulations suitable for vaginal administration may be presented as pessaries, creams, gels, pastes, foams or spray formulations containing in addition to the agent, such carriers as are known in the art to be appropriate.
Formulations suitable for nasal administration, wherein the carrier is a solid, include coarse powders having a particle size, for example, in the range of about 20 to about 500 microns which are administered in the manner in which snuff is taken, i.e., by rapid inhalation (e.g., forced) through the nasal passage from a container of the powder held close up to the nose. Other suitable formulations wherein the carrier is a liquid for administration include, but are not limited to, nasal sprays, drops, or aerosols by nebulizer, and include aqueous or oily solutions of the agents.
Formulations suitable for parenteral administration include aqueous and non-aqueous isotonic sterile injection solutions which may contain antioxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents, and liposomes or other microparticulate systems which are designed to target the compound to blood components or one or more organs. In some embodiments, the formulations are presented/formulated in unit-dose or multi-dose sealed containers, for example, ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
Preferred unit dosage formulations are those containing a daily dose or unit, daily subdose, as herein above-recited, or an appropriate fraction thereof, of an agent.
It should be understood that in addition to the ingredients particularly mentioned above, the formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example, those suitable for oral administration may include such further agents as sweeteners, thickeners and flavoring agents. It also is intended that the agents, compositions and methods of this invention be combined with other suitable compositions and therapies. Still other formulations optionally include food additives (suitable sweeteners, flavorings, colorings, etc.), phytonutrients (e.g., flax seed oil), minerals (e.g., Ca, Fe, K, etc.), vitamins, and other acceptable compositions (e.g., conjugated linoelic acid), extenders, and stabilizers, etc.
C. Exemplary Administration Routes and Dosing Considerations
Various delivery systems are known and can be used to administer therapeutic agents (e.g., exemplary compounds as described above) of the present invention, e.g., encapsulation in liposomes, microparticles, microcapsules, receptor-mediated endocytosis, and the like. Methods of delivery include, but are not limited to, intra-arterial, intra-muscular, intravenous, intranasal, and oral routes. In specific embodiments, it may be desirable to administer the pharmaceutical compositions of the invention locally to the area in need of treatment; this may be achieved by, for example, and not by way of limitation, local infusion during surgery, injection, or by means of a catheter.
The agents identified can be administered to subjects or individuals susceptible to or at risk of developing pathological growth of target cells and correlated conditions. When the agent is administered to a subject such as a mouse, a rat or a human patient, the agent can be added to a pharmaceutically acceptable carrier and systemically or topically administered to the subject. To identify patients that can be beneficially treated, a tissue sample is removed from the patient and the cells are assayed for sensitivity to the agent.
Therapeutic amounts are empirically determined and vary with the pathology being treated, the subject being treated and the efficacy and toxicity of the agent. When delivered to an animal, the method is useful to further confirm efficacy of the agent. One example of an animal model is MLR/MpJ-lpr/lpr (“MLR-lpr”) (available from Jackson Laboratories, Bar Harbor, Me.). MLR-lpr mice develop systemic autoimmune disease. Alternatively, other animal models can be developed by inducing tumor growth, for example, by subcutaneously inoculating nude mice with about 105 to about 109 hyperproliferative, cancer or target cells as defined herein. When the tumor is established, the compounds described herein are administered, for example, by subcutaneous injection around the tumor. Tumor measurements to determine reduction of tumor size are made in two dimensions using venier calipers twice a week. Other animal models may also be employed as appropriate. Such animal models for the above-described diseases and conditions are well-known in the art.
In some embodiments, in vivo administration is effected in one dose, continuously or intermittently throughout the course of treatment. Methods of determining the most effective means and dosage of administration are well known to those of skill in the art and vary with the composition used for therapy, the purpose of the therapy, the target cell being treated, and the subject being treated. Single or multiple administrations are carried out with the dose level and pattern being selected by the treating physician.
Suitable dosage formulations and methods of administering the agents are readily determined by those of skill in the art. Preferably, the compounds are administered at about 0.01 mg/kg to about 200 mg/kg, more preferably at about 0.1 mg/kg to about 100 mg/kg, even more preferably at about 0.5 mg/kg to about 50 mg/kg. When the compounds described herein are co-administered with another agent (e.g., as sensitizing agents), the effective amount may be less than when the agent is used alone.
The pharmaceutical compositions can be administered orally, intranasally, parenterally or by inhalation therapy, and may take the form of tablets, lozenges, granules, capsules, pills, ampoules, suppositories or aerosol form. They may also take the form of suspensions, solutions and emulsions of the active ingredient in aqueous or non-aqueous diluents, syrups, granulates or powders. In addition to an agent of the present invention, the pharmaceutical compositions can also contain other pharmaceutically active compounds or a plurality of compounds of the invention.
More particularly, an agent of the present invention also referred to herein as the active ingredient, may be administered for therapy by any suitable route including, but not limited to, oral, rectal, nasal, topical (including, but not limited to, transdermal, aerosol, buccal and sublingual), vaginal, parental (including, but not limited to, subcutaneous, intramuscular, intravenous and intradermal) and pulmonary. It is also appreciated that the preferred route varies with the condition and age of the recipient, and the disease being treated.
Ideally, the agent should be administered to achieve peak concentrations of the active compound at sites of disease. This may be achieved, for example, by the intravenous injection of the agent, optionally in saline, or by oral administration, for example, as a tablet, capsule or syrup containing the active ingredient.
Desirable blood levels of the agent may be maintained by a continuous infusion to provide a therapeutic amount of the active ingredient within disease tissue. The use of operative combinations is contemplated to provide therapeutic combinations requiring a lower total dosage of each component than may be required when each individual therapeutic compound or drug is used alone, thereby reducing adverse effects.
D. Exemplary Co-Administration Routes and Dosing Considerations
The invention also includes methods involving co-administration of the compounds described herein with one or more additional active agents. Indeed, it is a further aspect of this invention to provide methods for enhancing prior art therapies and/or pharmaceutical compositions by co-administering a compound of this invention. In co-administration procedures, the agents may be administered concurrently or sequentially. In one embodiment, the compounds described herein are administered prior to the other active agent(s). The pharmaceutical formulations and modes of administration may be any of those described above. In addition, the two or more co-administered chemical agents, biological agents or radiation may each be administered using different modes or different formulations.
The agent or agents to be co-administered depend on the type of condition being treated. For example, when the condition being treated is cancer, the additional agent can be a chemotherapeutic agent or radiation. When the condition being treated is an immune disorder, the additional agent can be an immunosuppressant or an anti-inflammatory agent. When the condition being treated is chronic inflammation, the additional agent can be an anti-inflammatory agent. The additional agents to be co-administered, such as anticancer, immunosuppressant, anti-inflammatory, can be any of the well-known agents in the art, including, but not limited to, those that are currently in clinical use. The determination of appropriate type and dosage of radiation treatment is also within the skill in the art or can be determined with relative ease.
Treatment of the various conditions associated with abnormal apoptosis is generally limited by the following two major factors: (1) the development of drug resistance and (2) the toxicity of known therapeutic agents. In certain cancers, for example, resistance to chemicals and radiation therapy has been shown to be associated with inhibition of apoptosis. Some therapeutic agents have deleterious side effects, including non-specific lymphotoxicity, renal and bone marrow toxicity.
The methods described herein address both these problems. Drug resistance, where increasing dosages are required to achieve therapeutic benefit, is overcome by co-administering the compounds described herein with the known agent. The compounds described herein sensitize target cells to known agents (and vice versa) and, accordingly, less of these agents are needed to achieve a therapeutic benefit.
The sensitizing function of the claimed compounds also addresses the problems associated with toxic effects of known therapeutics. In instances where the known agent is toxic, it is desirable to limit the dosages administered in all cases, and particularly in those cases where drug resistance has increased the requisite dosage. When the claimed compounds are co-administered with the known agent, they reduce the dosage required which, in turn, reduces the deleterious effects.
EXAMPLES
The invention now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention, and are not intended to limit the invention.
Example 1
Preparation of Pyrazolyl Guanidine Compounds
Described below are exemplary, general synthetic procedures for making pyrazolyl guanidine compounds, along with an exemplary synthetic procedure for making the specific pyrazolyl guanidine compound (Z)-4-chloro-N-(((3,4,5-trifluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)benzamide.
Part I: General Method for Making Pyrazolyl Guandine Compounds




Guanidines can be prepared from an acid chloride, a first amine, and a second amine using a three-step procedure. First, the requisite acid chloride is combined with potassium thiocyanate in an organic solvent, and this mixture is stirred at ambient temperature for 1-4 hours. The resulting mixture is concentrated in vacuo and used immediately.
In a second step, an appropriate first amine (RNH2) is dissolved in an organic solvent, such as methylene chloride, at ambient temperature and the acyl isothiocyanate from the first step is added. The resulting mixture is stirred at ambient temperature for 8-16 hours. The solvents are evaporated in vacuo and the resulting residue treated with a warm non-polar organic solvent, then allowed to cool and collected by filtration. The collected residue is rinsed with a non-polar organic solvent and dried. The resulting residue can be used without further purification. Alternatively, the first amine in the form of a hydrochloride salt is dissolved in an organic solvent and treated with a hindered organic base such as triethylamine then stirred at ambient temperature for 1-4 hours. The acyl isothiocyanate from step 1 is then added and the reaction mixture stirred at ambient temperature for 8-16 hours. The solvents are removed in vacuo and the resulting residue is purified by chromatography.
In the third step, the acyl thiourea from step 2 and an appropriate second amine (R1—NH2) are dissolved in a polar organic solvent such as dimethylformamide at ambient temperature to form a mixture. To this mixture, 1-ethyl-2′,2′-dimethylaminopropylcarbodiimide is added and the resulting mixture is stirred until the reaction appears complete by HPLC analysis of aliquots of the reaction mixture. Typical reaction times range from 30 minutes to 12 hours, and the reaction mixture may be heated (e.g., to approximately 60° C.) to accelerate the reaction. Once the reaction appears to be complete by HPLC analysis, the reaction mixture is diluted with an organic solvent (such as ethylacetate), washed with water, washed with brine, and the organic layer is dried over an appropriate drying agent, filtered, and the solvents removed under reduced pressure. The desired product can be purified by chromatography if necessary.
Part II: Exemplary Synthetic Procedure for Preparing Pyrazolyl Compound (Z)-4-Chloro-N-(((3,4,5-trifluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)benzamide
Step A: Representative Procedure for Preparing a Substituted Benzoyl Isothiocyanate in Situ and Conversion to a Thiourea




To a suspension of potassium thiocyanate (KNCS) (1.22 g, 12.57 mmol) in dichloromethane (60 mL) was added 400 μL of PEG-400 followed by 4-chlorobenzoyl chloride (2.00 g, 11.43 mmol). The suspension was heated to 40° C. for 2 h, then cooled to room temperature and filtered into an addition funnel. This solution was added dropwise into an ice cold solution of 5-amino-3-trifluoromethylpyrazole (1.73 g, 11.43 mmol) in dichloromethane (60 mL). The reaction was stirred for 20 minutes following the complete addition, and then it was concentrated in vacuo delivering the product as a yellow solid (3.76 g, 94% yield). 1HNMR (400 MHz, DMSO-d6) δ 13.93 (bs, 1H), 12.88 (bs, 1H), 12.00 (bs, 1H), 7.97 (d, 2H, J=8.4 Hz), 7.61 (d, 2H, J=8.4 Hz), 7.16 (bs, 1H).
Step B: Representative Procedure for the Coupling of a Thiourea to an Amine




4-Chloro-N-((3-(trifluoromethyl)-1H-pyrazol-5-yl)carbamothioyl)benzamide (500 mg, 1.43 mmol) was dissolved in methanol (5 mL) and 3,4,5-trifluorobenzylamine (462 mg, 2.87 mmol) was added followed by N1-((ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine hydrochloride (550 mg, 2.87 mmol). The reaction was stirred at 50° C. for 30 minutes. The crude mixture was then partitioned between water and ethyl acetate. The organic layer was washed with brine, and dried over sodium sulfate, then purified by chromatography (gradient:
95:5 hexanes:EtOAc to 7:3 hexanes:EtOAc). (Z)-4-Chloro-N-(((3,4,5-trifluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)benzamide was obtained as a solid (221 mg, 32% yield). MS (ES+) 475.8.
Example 2
Preparation of (Z)—N-((Tert-butylamino)(3-(trifluoromethyl)-1H-pyrazol-5-ylamino)methylene)-4-chlorobenzamide
Part I: Exemplary Procedure for Preparation of a Substituted Benzoyl Isothiocyanate in Situ and Conversion to a Thiourea




To a suspension of potassium thiocyanate (KNCS) (611 mg, 6.29 mmol) in dichloromethane (30 mL) was added 200 μL of PEG-400 followed by 4-chlorobenzoyl chloride (1.00 g, 5.71 mmol). The suspension was heated to 40° C. for 2 h, then cooled to room temperature and filtered into an addition funnel. The solution was added dropwise into an ice cold solution of t-butylamine (418 mg, 5.71 mmol) in dichloromethane (30 mL). The reaction was stirred for 20 minutes following the complete addition, and then it was concentrated in vacuo delivering N-(tert-butylcarbamothioyl)-4-chlorobenzamide as a yellow solid (1.49 g, 96% yield). 1HNMR (400 MHz, DMSO-d6) δ 11.11 (s, 1H), 11.02 (s, 1H), 7.87 (d, 2H, J=8.8 Hz), 7.54 (d, 2H, J=8.4 Hz), 1.51 (s, 9H).
Part II: Exemplary Procedure for Coupling of a Thiourea to an Amine




A solution of N-(tert-butylcarbamothioyl)-4-chlorobenzamide (150 mg, 0.55 mmol), 5-amino-3-trifluoromethylpyrazole (92 mg, 0.61 mmol), and N1-((ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine hydrochloride (127 mg, 0.67 mmol) in dimethylformamide (5.5 mL) was heated to 65° C. for 30 mins, then allowed to cool to room temperature. The mixture was diluted with EtOAc and washed with water twice, then brine. The organic layer was dried over sodium sulfate and concentrated to provide the title compound in crude form. Purification of the crude by chromatography (gradient: 95:5 hexanes:EtOAc to 70:30 hexanes:EtOAc) provided (Z)—N-((tert-butylamino)(3-(trifluoromethyl)-1H-pyrazol-5-ylamino)methylene)-4-chlorobenzamide as a solid (80 mg, 37% yield). MS (ES+) 387.9.
Example 3
Preparation of (Z)—N-((2-Chloro-4-fluorobenzylamino)(3-(trifluoromethyl)-1H-pyrazol-5-ylamino)methylene)-3,4-difluorobenzamide
Part I: Preparation of N-(2-Chloro-4-fluorobenzylcarbamothioyl)-3,4-difluorobenzamide




To a solution of 3,4-diflorobenzoyl chloride (45 mL, 358 mmol) in acetonitrile (1.43 L) was added solid potassium thiocyanate (KNCS) (38.2 g, 393 mmol). The suspension was stirred to 0° C. for 10 min and then the cooling bath was removed. The suspension was stirred at room temperature for an additional 20 min. A solution of 2-chloro-4-fluorobenzylamine (57.1 g, 358 mmol) in acetonitrile (150 mL) was added over 5 minutes. The resulting suspension was stirred at room temperature for 3 hours. The reaction mixture was diluted with water (1.4 L) and stirred for 1 hour. The suspension was filtered, rinsed with water (150 mL) and dried in vacuo at 60° C. to afford N-((2-chloro-4-fluorobenzyl)carbamothioyl)-3,4-difluorobenzamide as a yellow solid (98.3 g, 77% yield). 1HNMR (400 MHz, DMSO-d6) δ 11.56 (bs, 1H), 11.12 (t, 1H, J=5.7 Hz), 8.00 (m, 1H), 7.79 (m, 1H), 7.54 (m, 1H), 7.44 (m, 2H), 7.19 (m, 1H), 4.85 (d, 2H, J=5.7 Hz).
Part II: Preparation of (Z)—N-((2-Chloro-4-fluorobenzylamino)(3-(trifluoromethyl)-1H-pyrazol-5-ylamino)methylene)-3,4-difluorobenzamide




N-((2-Chloro-4-fluorobenzyl)carbamothioyl)-3,4-difluorobenzamide (98.0 g, 273 mmol) and 5-amino-3-trifluoromethylpyrazole (41.3 g, 273 mmol) were dissolved in THF (546 mL). N1-((Ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine hydrochloride (57.6 g, 300 mmol) was added and the reaction was stirred at room temperature for 1 h and then heated to 40° C. for 15 h. The crude mixture was diluted with water (500 mL) and isopropyl acetate (500 mL). The organic layer was washed with brine (500 mL), dried over sodium sulfate, and concentrated. The residue was diluted with isopropyl acetate (1.3 L) and heated to 65° C. for 1 hour. The solution was allowed to cool to room temperature, filtered and the filtrate was concentrated. The residue was heated to 70° C. in acetonitrile (900 mL) and then allowed to cool to room temperature. The resulting suspension was filtered, rinsed with acetonitrile (200 mL) and dried in vacuo at 60° C. to afford 55 g (43%) of (Z)—N-(((2-chloro-4-fluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-3,4-difluorobenzamide as a white solid. 1HNMR (400 MHz, MeOH-d4) δ 7.95 (bs, 2H), 7.50 (t, 1H, J=7.2 Hz), 7.26 (m, 2H), 7.06 (t, 1H, J=7.5 Hz), 6.56 (s, 1H), 4.84 (s, 2H).
Example 4
Alternative Procedure for Making Pyrazolyl Guanidine Compounds
Described below is an alternative exemplary general synthetic procedure for making pyrazolyl guanidine compounds, along with an exemplary synthetic procedure for making the specific pyrazolyl guanidine compound N-(((3-(4-(benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)amino)(cyclopentylamino)methylene)-4-(trifluoromethyl)benzamide.
Part I: Alternative General Method for Making Pyrazolyl Guandine Compounds




Acyl guanidines can be prepared from an acid chloride, a first amine, and a second amine using a three-step, 2-pot procedure. First, the requisite acid chloride is combined with a slight molar excess of potassium thiocyanate in a polar aprotic solvent (such as acetonitrile) at a temperature in the range of from about 0° C. to room temperature. The resulting mixture is stirred for a time period ranging from 15 minutes to 2 hours to provide a reaction mixture containing the acyl isothiocyanate synthetic intermediate compound. This reaction mixture may be used directly in the next reaction or filtered to remove the potassium chloride generated during this first step.
In a second step, the acyl isothiocyanate compound is combined with a second amine (which may be dissolved in a solvent) to provide a reaction mixture that is stirred for a time period ranging from 15 minutes to 2 hours to produce an acyl thiourea product. The acyl thiourea product often precipitates from the reaction mixture and may be collected by filtration. Water may be added to the reaction mixture (typically in amount equal to the volume of organic solvent) to facilitate collection of the acyl thiourea product. The acyl thiourea product is dried in vacuo at approximately ambient temperature.
In a third step, the acyl thiourea product is combined with a coupling agent (such as N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDCI)) and a second amine to produce the acyl guanidine. All starting materials for this reaction may be combined prior to heating the reaction mixture or the EDCI may be added last, once the temperature of the reaction mixture has been raised. Alternatively, the second amine may be added last after combining the thiourea and the EDCI. Once all the starting materials have been combined, the reaction mixture is generally stirred for a time period ranging from about 30 minutes to about 3 hours while the reaction mixture is heated to a temperature ranging from about 45° C. to about 70° C.
Part II: Exemplary Synthetic Procedure for Preparing Pyrazolyl Compound N-(((3-(4-(benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)amino)(cyclopentylamino)methylene)-4-(trifluoromethyl)benzamide
Step A: Preparation of 4-(Benzyloxy)-2-fluorobenzoyl chloride




4-Benzyloxy-2-fluoro-benzoic acid (2 g, 8.12 mmol) was suspended in thionyl chloride (5.9 mL) and heated to reflux for 30 minutes. The reaction mixture was then concentrated under reduced pressure, and the resulting residue was dissolved in toluene and re-concentrated twice to remove residual thionyl chloride. This procedure provided the title compound in crude form, which was used in the next reaction without further purification.
Step B: Preparation of 3-(4-(Benzyloxy)-2-fluorophenyl)-3-oxopropanenitrile




Acetonitrile (1.66 mL, 31.7 mmol) was dissolved in dry THF (40 mL) and the mixture was cooled to −78 C. Butyllithium (2.5 M, 9.5 mL, 23.8 mmol) was added dropwise, and the resulting reaction mixture was stirred for 15 minutes. A solution of 4-(benzyloxy)-2-fluorobenzoyl chloride (2.1 g, 7.93 mmol) in THF was then added dropwise to the reaction mixture, and the resulting reaction mixture was stirred at −78 C for 40 minutes. Saturated ammonium chloride solution was then added carefully to the reaction mixture, and the resulting mixture was allowed to warm to room temperature. Next, the mixture was partitioned between water and EtOAc, and the organic portion was washed further with brine, then dried over sodium sulfate, and concentrated onto silica gel and purified by chromatography (gradient: 9:1 EtOAc:hexanes to 1:1 EtOAc:hexanes to 100% ethyl acetate) to give the title compound in 79% yield.
Step C: Preparation of 3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-amine




3-(4-(Benzyloxy)-2-fluorophenyl)-3-oxopropanenitrile (1.7 g, 6.3 mmol) was dissolved in ethanol (21 mL), and then hydrazine (0.22 mL, 6.9 mmol) was added. The reaction mixture was stirred at room temperature for about one hour, and the progress of the reaction was followed by thin-layer chromatography. If no progress had been made, the temperature was gradually increased until the reaction began proceeding. As soon as the reaction appeared complete by thin-layer chromatography, the crude material was concentrated in vacuo and purified by chromatography to provide the title compound 97% yield.
Step D: Preparation of N-(3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-ylcarbamothioyl)-4-(trifluoromethyl)benzamide




4-Trifluoromethylbenzoyl chloride (590 mg, 2.83 mmol) was dissolved in acetonitrile ( ̃30 mL) and potassium thiocyanate (302 mg, 3.22 mmol) was added to provide a reaction mixture. The reaction mixture was stirred at room temperature for 30 minutes, and then 3-(4-benzyloxy-2-fluoro-phenyl)-1H-pyrazol-5-amine (801 mg, 2.83 mmol) was added to the reaction mixture. Next, the reaction mixture was stirred at room temperature overnight, then concentrated in vacuo, slurried with water, and collected by filtration. The wet solid was held under heated vacuum for 24 hours to provide the title compound in 81% yield.
Step E: Preparation of N-(((3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)amino)(cyclopentylamino)methylene)-4-(trifluoromethyl)benzamide




N-((3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)carbamothioyl)-4-(trifluoromethyl)benzamide (154 mg, 0.3 mmol) and EDCI (63 mg, 0.33 mmol) were suspended in THF (4 mL) and cyclopentylamine (0.044 mL, 0.45 mmol) was added. The resulting mixture was stirred at 50° C. for 45 minutes, then concentrated onto silica gel and purified by chromatography (gradient: 9:1 hexanes:EtOAc to 6:4 hexanes:EtOAc) to provide the title compound in 53% yield.
Step F: Preparation of N-(((3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)amino)(cyclopentylamino)methylene)-4-(trifluoromethyl)benzamide




N-(((3-(4-(Benzyloxy)-2-fluorophenyl)-1H-pyrazol-5-yl)amino)(cyclopentylamino)methylene)-4-(trifluoromethyl)benzamide (90 mg, 0.16 mmol) was dissolved in methanol (2 mL), and nitrogen gas was bubbled through briefly. 10% Palladium on carbon (84 mg) was then added to the reaction mixture, followed by the addition of ammonium formate (50 mg, 0.8 mmol). The reaction vessel was sealed and then heated to 65° C. for 1 hour. Next, the reaction mixture was cooled, and then filtered through a plug of celite. The filtrate was concentrated onto silica gel, and the product purified by chromatography to give the title compound in 12% yield.
Example 5
Pyrazolyl Guanidine Compounds & Characterization Data
Compounds in Table 2 below were prepared based on the procedures described in Examples 1-4 and procedures described in the detailed description. Starting materials can be obtained from commercial sources (e.g., acid chloride: 4-chlorobenzoyl chloride, 3-chlorobenzoyl chloride, and 4-fluorobenzoyl chloride; first amine compound: 2-trifluoromethylaniline, 2-tert-butylaniline, 4-fluoro-2-chlorobenzylamine, cycloheptylamine, tetrahydro-2H-pyran-4-amine; and second amine: 3-(trifluoromethyl)-1H-pyrazol-5-amine, 3-methyl-1H-pyrazol-5-amine, 3-(difluoromethyl)-1H-pyrazol-5-amine) or readily prepared from commercially available materials. Furthermore, exemplary compounds were characterized by high performance liquid chromatography (HPLC), mass spectrometry (MS) and/or 1H nuclear magnetic resonance spectroscopy. Unless indicated otherwise, mass spectral data in Table 2 was collected using electrospray ionization in the positive ion mode. The symbol “(M-H)” indicates that mass spectral data was collected in the negative ion mode. The HPLC method and retention time, along with mass spectral data are provided in Table 2 below. HPLC methods used are as follows: Method A conditions were Waters C-18 column, 4.6×150 mm, 3.5 micron, 23° C., 1.0 mL/min, 1 min 25% MeCN in H2O (0.1% TFA), 10 min gradient of 25%-95% MeCN in H2O (0.1% TFA), 95% MeCN in H2O (0.1% TFA) for 5 min, and then equilibration to 25% MeCN in H2O (0.1% TFA) over 2.0 min; Method B conditions were Agilent Zorbax C-18 column, 4.6×50 mm, 1.8 micron, 23° C., 1.0 mL/min, 1 min 25% MeCN in H2O (0.1% TFA), 5 min gradient of 25%-95% MeCN in H2O (0.1% TFA), 1 min at 95% MeCN in H2O (0.1% TFA), and then equilibration to 25% MeCN in H2O (0.1% TFA) over 1.0 min; Method C conditions were Phenomenex Kinetex C18 (3.0 mm×50 mm), 2.6 micron, 58° C., 1.5 mL/min, 4 min gradient 5% MeCN (0.1% TFA) in H2O (0.1% TFA) to 100% MeCN (0.1% TFA), 100% MeCN (0.1% TFA) for 0.5 min, and then equilibration to 5% MeCN (0.1% TFA) in H2O (0.1% TFA) over 1.5 min; Method D conditions were Phenomenex Kinetex C18 (3.0 mm×50 mm), 2.6 micron, 40° C., 1.5 mL/min, 4 min gradient 5% MeCN (0.1% TFA) in H2O (0.1% TFA) to 100% MeCN (0.1% TFA), 100% MeCN (0.1% TFA) for 0.5 min, and then equilibration to 5% MeCN (0.1% TFA) in H2O (0.1% TFA) over 1.5 min; and Method E conditions were Waters Symmetry C18 (4.6 mm×150 mm), 3.5 micron, 26° C., 2.0 mL/min, 1 min 25% MeCN (0.05% TFA) in H2O (0.05% TFA), 7 min gradient of 25%-95% MeCN (0.05% TFA) in H2O (0.05% TFA), 95% MeCN (0.05% TFA) in H2O (0.05% TFA) for 2 min, and then equilibration to 25% MeCN (0.05% TFA) in H2O (0.05% TFA) over 2.0 min. The phrase “MeCN (0.05% TFA)” is art-recognized and refers to acetonitrile containing 0.05% wt/wt trifluoroacetic acid. The symbol “NA” indicates that no data was available.
1H nuclear magnetic resonance data for exemplary compounds is provided in Table 3.












TABLE 2







Calculated


HPLC


Compound

MW
MS
HPLC
Retention


No.
Chemical Structure
(g/mol)
(m/z)
Method
Time (min)




















A-1 




447.3
447.9
A
11.27


 


A-2 




523.4
523.9
B
6.98


 


A-3 




492.2
491.7
B
6.96


 


A-4 




387.8
387.9
B
6.59


 


A-5 




413.7
413.8
B
6.33


 


A-6 




399.8
399.9
B
6.17


 


A-7 




425.8
425.9
B
5.85


 


A-8 




491.1
492
C
2.86


 


A-9 




489.1
490
C
2.81


 


A-10 




489.1
490
C
2.72


 


A-11 




415.1
416
C
2.65


 


A-12 




389.1
390
C
2.74


 


A-13 




421.3
388
C
2.66


 


A-14 




461.4
461.9
B
6.46


 


A-15 




413.9
413.95, 415.94
A
8.62


 


A-16 




401.8
401.87, 403.87
A
11.14


 


A-17 




387.9
387.94, 389.90
A
8.95


 


A-18 




507.8
508
C
2.75


 


A-19 




507.8
508
C
2.7


 


A-20 




507.8
508
C
2.72


 


A-21 




503.8
508
C
2.77


 


A-22 




503.8
504
C
2.9


 


A-23 




475.8
504
C
2.8


 


A-24 




475.8
476
C
2.61


 


A-25 




475.8
476
C
2.57


 


A-26 




475.8
476
C
2.59


 


A-27 




475.8
476
C
2.63


 


A-28 




471.8
472
C
2.76


 


A-29 




471.8
472
C
2.67


 


A-30 




474.2
474
C
2.53


 


A-31 




474.2
474
C
2.51


 


A-32 




474.2
474
C
2.53


 


A-33 




474.2
474
C
2.57


 


A-34 




470.3
470
C
2.7


 


A-35 




470.3
470
C
2.62


 


A-36 




470.3
470
C
2.59


 


A-37 




344.8
344.9
B
6.49


 


A-38 




381.9
381.93,  383.89
A
12.59


 


A-39 




477.3
477.8
B
6.53


 


A-40 




405.78
406
B
7.04


 


A-41 




429.4
NA
B
6.18


 


A-42 




401.8
338.00,  401.91,  423.85
B
5.46


 


A-43 




403.4
340.02,  403.93,  425.87
B
5.73


 


A-44 




403.4
403.93,  425.87
B
7.58


 


A-45 




407.8
407.79
B
7


 


A-46 




471.8
471.84,  493.78
B
6.82


 


A-47 




471.8
471.78, 493.72
B
6.82


 


A-48 




507.8
507.82
B
7.07


 


A-49 




447.4
447.94
B
6.29


 


A-50 




447.4
447.94
B
6.8


 


A-51 




435.4
372.01, 435.92, 457.86
B
6.02


 


A-52 




443.76
443.76,  445.79
A
12.35


 


A-53 




403.35
403.91
A
11.28


 


A-54 




369.8
369.99,  372.01
A
10.43


 


A-55 




355.77
355.98,  357.97
A
6.91


 


A-56 




456.25
455.89,  457.86
A
9.93


 


A-57 




442.22
441.93,  443.89
A
12.68


 


A-58 




389.32
389.99
A
7.91


 


A-59 




489.8
489.87,  491.88
A
10.9


 


A-60 




475.77
475.89,  477.85
A
12.67


 


A-61 




457.78
457.93,  459.95
A
10.45


 


A-62 




371.33
372.04
A
10.76


 


A-63 




357.31
358.03
A
7.26


 


A-64 




403.35
403.9
A
10.6


 


A-65 




387.79
387.83,  389.83
A
11.92


 


A-66 




417.38
417.97
A
8.52


 


A-67 




415.36
415.91
A
8.05


 


A-68 




417.38
NA
NA
NA


 


A-69 




383.82
383.92,  385.88
A
7.93


 


A-70 




392.38
392.92
A
9.37


 


A-71 




378.35
378.87
A
10.79


 


A-72 




392.38
392.93
A
9.46


 


A-73 




378.35
378.9
A
10.75


 


A-74 




417.38
417.97
A
11.08


 


A-75 




433.35
433.91
A
9.79


 


A-76 




435.37
435.92
A
10.42


 


A-77 




421.34
421.94
A
12.15


 


A-78 




435.37
435.97
A
12.58


 


A-79 




422.33
422.89
A
11.57


 


A-80 




436.35
436.9
A
11.19


 


A-81 




464.8
464.88,  466.84
A
10.9


 


A-82 




464.8
464.88,  466.84
A
10.87


 


A-83 




489.8
489.86,  491.82
A
10.58


 


A-84 




368.36
369.03
A
5.9


 


A-85 




382.38
383.03
A
6.12


 


A-86 




454.81
454.87,  456.85
A
6.54


 


A-87 




443.41
443.94
A
9.11


 


A-88 




385.36
385.97
A
8.42


 


A-89 




503.84
504
C
2.8


 


A-90 




407.32
508
C
2.12


 


A-91 




419.33
420
C
2.28


 


A-92 




433.36
434
C
2.28


 


A-93 




435.38
436
C
2.82


 


A-94 




475.44
476
C
2.63


 


A-95 




449.36
450
C
2.09


 


A-96 




449.36
450
C
2.17


 


A-97 




437.35
438
C
2.13


 


A-98 




375.3
376
C
1.94


 


A-99 




387.32
388
C
2.03


 


A-100




401.34
402
C
2.11


 


A-101




403.36
404
C
2.67


 


A-102




443.42
444
C
2.51


 


A-103




403.31
404
C
1.92


 


A-104




417.34
418
C
1.92


 


A-105




417.34
418
C
1.99


 


A-106




405.33
406
C
1.95


 


A-107




373.77
374
C
1.9


 


A-108




385.78
386
C
1.98


 


A-109




441.89
442
C
2.43


 


A-110




415.81
416
C
1.93


 


A-111




403.79
404
C
1.91


 


A-112




449.84
450
C
1.99


 


A-113




391.76
392
C
2.13


 


A-114




403.77
404
C
2.23


 


A-115




417.8
418.40
C
2.31


 


A-116




419.81
420
C
2.89


 


A-117




459.88
460
C
2.7


 


A-118




433.8
434
C
2.1


 


A-119




433.8
434
C
2.19


 


A-120




421.79
422
C
2.24


 


A-121




467.83
468
C
2.17


 


A-122




399.81
400
C
2.09


 


A-123




401.82
402
C
2.67


 


A-124




415.81
416
C
1.9


 


A-125




367.37
390  (M + Na )
E
5.84


 


A-126




501.86
502
D
3.26


 


A-127




390.41
391
E
4.9


 


A-128




463.88
464
NA
NA


 


A-129




430.33
431
D
2.71


 


A-130




430.33
431
D
2.69


 


A-131




430.33
431
D
2.6


 


A-132




430.33
431
D
2.57


 


A-133




413.88
414
D
2.49


 


A-134




413.88
414
D
2.37


 


A-135




413.88
414
D
2.36


 


A-136




431.87
432
D
2.75


 


A-137




431.87
432
D
2.72


 


A-138




463.88
464
D
2.83


 


A-139




459.88
460
D
2.21


 


A-140




441.89
442
D
2.16


 


A-141




413.88
414
D
2.77


 


A-142




518.32
518
D
2.83


 


A-143




500.33
500
D
2.79


 


A-144




492.33
492
D
3.28


 


A-145




504.29
504
D
3.31


 


A-146




450.27
450
D
3.11


 


A-147




464.3
464
D
3.24


 


A-148




516.33
516
D
3.28


 


A-149




520.75
522
D
3.42


 


A-150




500.33
500
D
3.38


 


A-151




501.86
502
D
3.22


 


A-152




431.87
432
D
2.8


 


A-153




429.44
430
D
2.68


 


A-154




519.85
520
D
3.24


 


A-155




381.4
382.13,  404.13
B
5.169


 


A-156




395.41
396.04,  418.00
E
4.077


 


A-157




409.46
410.07,  432.04 
E
5.088


 


A-158




439.46
440.09
B
4.777


 


A-159




427.45
428.13,  450.10
B
5.66


 


A-160




479.88
434.05,  436.06
B
6.57


 


A-161




449.86
NA
NA
NA


 


A-162




449.86
450.10,  472.06
B
7.88


 


A-163




465.52
466.15,  488.05
B
4.89


 


A-164




465.52
466.15,  488.11
B
4.66


 


A-165




464.42
NA
NA
NA


 


A-166




448.42
NA
NA
NA


 


A-167




448.42
NA
NA
NA


 


A-168




498.34
NA
NA
NA


 


A-169




425.91
NA
NA
NA


 


A-170




425.91
NA
NA
NA


 


A-171




409.46
410.14
B
5.13


 


A-172




409.46
NA
NA
NA


 


A-173




463.39
464.07,  486.03
B
5.33


 


A-174




408.47
409.16
B
4.68


 


A-175




438.5
NA
NA
NA


 


A-176




408.47
409.16
B
4.7


 


A-177




408.47
409.16,  431.12
B
4.76


 


A-178




351.81
NA
NA
NA


 


A-179




391.75
NA
NA
NA


 


A-180




381.83
NA
NA
NA


 


A-181




351.81
NA
NA
NA


 


A-182




410.44
411.11,  433.14
B
4.95


 


A-183




407.31
NA
NA
NA


 


A-184




397.39
NA
NA
NA


 


A-185




379.38
NA
NA
NA


 


A-186




375.3
NA
NA
NA


 


A-187




349.38
NA
NA
NA


 


A-188




347.36
NA
NA
NA


 


A-189




367.37
NA
NA
NA


 


A-190




353.34
NA
NA
NA


 


A-191




335.35
NA
NA
NA


 


A-192




335.35
NA
NA
NA


 


A-193




381.38
NA
NA
NA


 


A-194




461.41
NA
NA
NA


 


A-195




445.41
NA
NA
NA


 


A-196




425.43
NA
NA
NA


 


A-197




433.36
NA
NA
NA


 


A-198




417.36
NA
NA
NA


 


A-199




415.37
NA
NA
NA


 


A-200




443.42
NA
NA
NA


 


A-201




439.46
NA
NA
NA


 


A-202




381.38
NA
NA
NA


 


A-203




411.45
NA
NA
NA


 


A-204




441.47
NA
NA
NA


 


A-205




427.45
NA
NA
NA


 


A-206




397.42
420.08
B
5.09


 


A-207




439.46
NA
NA
NA


 


A-208




439.46
NA
NA
NA


 


A-209




413.88
414.06, 436.06
B
5.3


 


A-210




473.9
NA
NA
NA


 


A-211




473.9
NA
NA
NA


 


A-212




473.9
NA
NA
NA


 


A-213




461.89
462.05
B
5.61


 


A-214




461.89
462.05
B
5.56


 


A-215




445.85
NA
NA
NA


 


A-216




417.84
418.00,  440.03
B
5.47


 


A-217




461.89
NA
NA
NA


 


A-218




447.87
448.02
B
5.49


 


A-219




431.87
432.03,  454.00 
B
5.75


 


A-220




431.87
432.03,  454.00
B
5.73


 


A-221




443.88
444.05,  466 .02
B
5.79


 


A-222




429.85
430.02,  451.98
B
5.64


 


A-223




445.85
NA
NA
NA


 


A-224




429.85
NA
NA
NA


 


A-225




431.87
432.03,  454.00
B
5.65


 


A-226




446.31
NA
NA
NA


 


A-227




461.89
NA
NA
NA


 


A-228




447.87
NA
NA
NA


 


A-229




443.88
NA
NA
NA


 


A-230




417.84
418.06,  440.03
B
5.27


 


A-231




429.85
430.02,  451.98
B
5.37


 


A-232




431.87
NA
NA
NA


 


A-233




431.87
432.03, 454.06
B
5.5


 


A-234




431.87
432.03, 454.06
B
5.44


 


A-235




431.41
432.10, 454.06
B
5.04


 


A-236




461.87
NA
NA
NA


 


A-237




493.91
NA
NA
NA


 


A-238




479.88
NA
NA
NA


 


A-239




475.89
NA
NA
NA


 


A-240




463.88
464.00, 485.97
B
5.86


 


A-241




449.86
449.97, 472.00
B
5.63


 


A-242




445.44
446.07, 468.03
B
5.24


 


A-243




427.42
428.07, 450.03
B
5.31


 


A-244




415.41
416.05, 438.08
B
5.24


 


A-245




401.39
402.08, 424.11
B
4.99


 


A-246




465.86
466.02, 487.98
B
5.64


 


A-247




479.88
NA
NA
NA


 


A-248




425.89
NA
NA
NA


 


A-249




413.88
414.03, 436.06
B
5.4


 


A-250




399.85
400.06, 422.03
B
5.14


 


A-251




411.86
NA
NA
NA


 


A-252




443.9
NA
NA
NA


 


A-253




425.89
NA
NA
NA


 


A-254




413.88
414.10, 436.06
B
5.33


 


A-255




399.85
400.06, 422.03
B
5.08


 


A-256




427.45
428.13, 450.10
B
5.18


 


A-257




397.42
398.12, 420.08
B
5.16


 


A-258




461.89
462.05, 484.02
B
5.66


 


A-259




413.4
414.03, 436.06
B
5.27


 


A-260




427.42
428.07, 451.07
B
5.44


 


A-261




415.41
416.05, 438.08
B
5.37


 


A-262




401.39
NA
NA
NA


 


A-263




445.44
446.07, 468.03
B
5.39


 


A-264




411.86
412.09, 434.05
B
5.26


 


A-265




443.9
NA
NA
NA


 


A-266




429.4
NA
NA
NA


 


A-267




445.44
446.13
B
5.41


 


A-268




445.44
446.07
B
5.43


 


A-269




427.45
NA
NA
NA


 


A-270




411.4
NA
NA
NA


 


A-271




427.45
NA
NA
NA


 


A-272




429.88
NA
NA
NA


 


A-273




465.86
NA
NA
NA


 


A-274




431.41
432.03, 454.06
B
5.15


 


A-275




431.41
NA
NA
NA


 


A-276




429.88
NA
NA
NA


 


A-277




461.89
NA
NA
NA


 


A-278




443.9
NA
NA
NA


 


A-279




427.45
NA
NA
NA


 


A-280




427.45
NA
NA
NA


 


A-281




459.46
NA
NA
NA


 


A-282




495.45
496.04, 518.01
B
5.6


 


A-283




493.91
494.03
B
5.8


 


A-284




447.84
447.95, 469.98
B
5.77


 


A-285




449.86
449.97, 472.00
B
5.87


 


A-286




435.83
436.00, 457.96
B
5.59


 


A-287




449.86
449.97, 472.00
B
5.84


 


A-288




475.89
497.99
B
7.42


 


A-289




461.87
461.99
B
5.89


 


A-290




449.86
472
B
7.31


 


A-291




477.43
478.04, 499.94
B
5.88


 


A-292




551.87
551.94, 553.89
B
7.06


 


A-293




465.42
466.02, 487.92
B
5.83


 


A-294




537.84
NA
NA
NA


 


A-295




479.45
NA
NA
NA


 


A-296




451.39
451.98, 473.95
B
5.6


 


A-297




465.42
466.02, 487.98
B
5.86


 


A-298




463.4
464.00, 485.90
B
5.75


 


A-299




465.42
465.95, 487.98
B
7.21


 


A-300




434.92
434.96
B
5.8


 


A-301




464.95
464.98
B
5.44


 


A-302




432.91
433.01, 454.97
B
5.55


 


A-303




420.89
420.99, 442.95
B
5.5


 


A-304




434.92
435.02, 456.99
B
5.83


 


A-305




406.87
406.95, 428.91
B
5.24


 


A-306




420.89
420.99, 442.95
B
5.53


 


A-307




418.88
418.97, 440.94
B
5.42


 


A-308




420.89
420.99, 442.95
B
6.85


 


A-309




434.44
434.96, 456.99
B
5.51


 


A-310




422.43
423.00, 444.96
B
5.46


 


A-311




436.46
436.96, 459.00
B
5.61


 


A-312




408.4
NA
NA
NA


 


A-313




422.43
423.00, 444.96
B
6.51


 


A-314




420.41
420.99, 442.95
B
5.37


 


A-315




422.43
NA
NA
NA


 


A-316




441.45
NA
NA
NA


 


A-317




515.89
NA
NA
NA


 


A-318




501.86
NA
NA
NA


 


A-319




415.41
NA
B
5.42


 


A-320




427.42
NA
NA
NA


 


A-321




443.46
NA
NA
NA


 


A-322




473.49
NA
NA
NA


 


A-323




429.44
NA
B
5.65


 


A-324




443.46
NA
NA
NA


 


A-325




429.44
NA
NA
NA


 


A-326




473.47
NA
NA
NA


 


A-327




547.91
NA
NA
NA


 


A-328




447.43
NA
NA
NA


 


A-329




459.44
NA
NA
NA


 


A-330




475.48
NA
NA
NA


 


A-331




505.51
NA
NA
NA


 


A-332




461.46
NA
NA
NA


 


A-333




475.48
NA
NA
NA


 


A-334




461.46
NA
NA
NA


 


A-335




513.89
513.91, 535.88
B
7.64


 


A-336




499.86
NA
NA
NA


 


A-337




482.82
NA
NA
NA


 


A-338




497.83
497.86, 519.83
B
6.47


 


A-339




411.38
433.98
B
6.47


 


A-340




427.44
428.00, 450.10
B
7.67


 


A-341




394.45
NA
NA
NA


 


A-342




437.47
NA
NA
NA


 


A-343




458.82
NA
NA
NA


 


A-344




493.91
NA
NA
NA


 


A-345




516.9
NA
NA
NA


 


A-346




463.88
463.94
B
6.29


 


A-347




447.84
447.95, 469.92
B
5.9


 


A-348




463.88
NA
NA
NA


 


A-349




463.88
NA
NA
NA


 


A-350




463.88
NA
NA
NA


 


A-351




463.88
464.00, 485.97
B
6.33


 


A-352




449.86
449.9, 471.87
B
5.98


 


A-353




461.87
NA
NA
NA


 


A-354




449.86
NA
NA
NA


 


A-355




475.89
NA
NA
NA


 


A-356




449.86
449.90, 471.93
B
6.03


 


A-357




503.83
NA
NA
NA


 


A-358




447.84
NA
NA
NA


 


A-359




435.83
NA
NA
NA


 


A-360




449.86
NA
NA
NA


 


A-361




447.84
NA
NA
NA


 


A-362




449.86
449.90, 471.87
B
5.83


 


A-363




449.4
449.90, 471.87
B
5.2


 


A-364




433.4
433.92, 455.88
B
5.39


 


A-365




445.41
445.87, 467.87
B
5.45


 


A-366




419.38
419.88, 441.91
B
5.14


 


A-367




431.41
NA
NA
NA


 


A-368




415.41
NA
NA
NA


 


A-369




427.42
427.94, 449.90
B
5.2


 


A-370




401.39
401.95, 423.98
B
4.84


 


A-371




443.45
NA
NA
NA


 


A-372




427.45
NA
NA
NA


 


A-373




427.45
NA
NA
NA


 


A-374




413.42
NA
NA
NA


 


A-375




458.51
457.9
B
5.13


 


A-376




447.87
447.95
B
5.4


 


A-377




431.87
431.9
B
5.62


 


A-378




443.88
443.92
B
5.68


 


A-379




417.84
417.87
B
5.37


 


A-380




464.32
463.87, 465.82
B
5.79


 


A-381




448.32
447.89, 449.84
B
5.99


 


A-382




460.33
459.85, 461.86
B
6.05


 


A-383




434.29
433.85, 435.87
B
5.74


 


A-384




429.88
NA
NA
NA


 


A-385




425.89
425.93, 427.87
B
5.45


 


A-386




399.85
399.93, 401.88
B
5.04


 


A-387




427.45
NA
NA
NA


 


A-388




409.43
NA
NA
NA


 


A-389




413.42
NA
NA
NA


 


A-390




383.39
NA
NA
NA


 


A-391




399.39
NA
NA
NA


 


A-392




475.49
NA
NA
NA


 


A-393




459.49
NA
NA
NA


 


A-394




473.5
NA
NA
NA


 


A-395




457.5
NA
NA
NA


 


A-396




497.44
497.86
B
6.71


 


A-397




481.45
NA
NA
NA


 


A-398




461.46
461.92
B
5.39


 


A-399




445.46
445.94
B
5.55


 


A-400




477.92
NA
NA
NA


 


A-401




461.92
NA
NA
NA


 


A-402




423.48
NA
NA
NA


 


A-403




433.4
455.95
B
7.43


 


A-404




387.33
NA
NA
NA


 


A-405




413.42
NA
NA
NA


 


A-406




399.34
NA
NA
NA


 


A-407




387.33
NA
NA
NA


 


A-408




438.33
NA
NA
NA


 


A-409




387.33
NA
NA
NA


 


A-410




387.33
NA
NA
NA


 


A-411




412.36
NA
NA
NA


 


A-412




429.37
NA
NA
NA


 


A-413




399.34
NA
NA
NA


 


A-414




409.34
431.9
B
8.27


 


A-415




413.37
NA
NA
NA


 


A-416




426.39
NA
NA
NA


 


A-417




461.46
NA
NA
NA


 


A-418




411.45
412.03, 434.01
A
9.71


 


A-419




505.83
505.94, 527.93
B
7.9


 


A-420




433.4
456.06
B
7.02


 


A-421




509.45
510.16
B
6.07


 


A-422




537.84
538.01, 540.02
B
8.12


 


A-423




465.42
466.07, 488.05
B
7.2


 


A-424




485.38
486.5
D
2.5


 


A-425




417.38
418.5
D
2.05


 


A-426




443.46
444.4
D
2.77


 


A-427




411.45
412.3
D
2.64


 


A-428




483.87
484.3
D
3.22


 


A-429




457.52
NA
E
7.09


 


A-430




457.52
NA
E
7.2


 


A-431




425.47
426.1
D
2.82


 


A-432




457.49
458.5
D
2.94


 


A-433




429.44
430.3
D
2.73


 


A-434




461.46
462.1
D
2.85


 


A-435




397.42
398.2
D
2.58


 


A-436




469.85
490.2
D
3.13


 


A-437




463.88
464
D
3.04


 


A-438




413.88
414
D
2.64


 


A-439




463.88
464
D
3.01


 


A-440




501.86
502
D
2.73


 


A-441




487.84
488
D
3.25


 


A-442




504.29
504
D
3.42


 


A-443




533.88
534
D
2.85


 


A-444




550.34
550.5
D
3.02


 


A-445




550.34
550.5
D
3


 


A-446




536.31
536
D
3.53


 


A-447




536.31
536
D
3.5


 


A-448




447.43
448
D
2.77


 


A-449




533.88
534
D
2.79


 


A-450




519.85
520.5
D
3.22


 


A-451




413.88
414
D
2.58


 


A-452




500.33
500
D
2.62


 


A-453




413.88
414.5
D
2.45


 


A-454




500.33
500
D
2.55


 


A-455




486.3
486.5
D
3.06


 


A-456




397.42
389
D
2.37


 


A-457




483.87
484.5
D
2.48


 


A-458




469.85
470.5
D
2.97


 


A-459




397.42
NA
D
2.24


 


A-460




483.87
484
D
2.36


 


A-461




469.85
470.5
D
2.87


 


A-462




471.91
472.5
D
2.21


 


A-463




476.33
476
D
2.33


 


A-464




476.33
476
D
2.31


 


A-465




475.44
476
D
2.11


 


A-466




457.45
458.5
D
2.08


 


A-467




443.9
444.5
D
2.68


 


A-468




448.32
448
D
2.91


 


A-469




448.32
448
D
2.91


 


A-470




427.9
428
D
2.82


 


A-471




530.35
530
D
2.82


 


A-472




534.77
536
D
2.92


 


A-473




534.77
536
D
2.9


 


A-474




514.35
514.5
D
2.83


 


A-475




520.75
522
D
3.39


 


A-476




513.9
514.5
D
2.54


 


A-477




518.32
518
D
2.78


 


A-478




518.32
518
D
2.8


 


A-479




497.9
498.5
D
2.65


 


A-480




499.87
500
D
3.03


 


A-481




531.89
532
D
3.14


 


A-482




486.3
486.5
D
3.31


 


A-483




483.87
484.5
D
2.63


 


A-484




465.52
466.26
A
6.49


 


A-485




437.42
460.23 (M +  Na + 1)+
A
7.45


 


A-486




395.43
396.11
A
6.95


 


A-487




449.52
448.29 (M − H)−
A
7.72


 


A-488




431.41
430.03 (M − H)−
E
8.68


 


A-489




415.41
414.07 (M − H)−
E
9.62


 


A-490




408.4
407.08 (M − H)−
E
8.93


 


A-491




473.52
427.06 (M − H)−
E
8.27


 


A-492




411.43
410.09 (M − H)−
E
8.24


 


A-493




426.44
425.08 (M − H)−
E
8.5


 


A-494




410.44
409.10 (M − H)−
E
9.6


 


A-495




426.44
425.08 (M − H)−
E
8.68


 


A-496




410.44
409.10 (M − H)−
E
10.65


 


A-497




473.52
427.06 (M − H)−
A
8.36


 


A-498




457.52
456.06 (M − H)−
A
9.8


 


A-499




457.52
456.08 (M − H)−
A
9.85


 


A-500




413.42
412.10 (M − H)−
A
8.76


 


A-501




459.44
458.04 (M − H)−
A
9.27


 


A-502




466.46
465.04 (M − H)−
A
9.79


 


A-503




489.8
489.96, 491.95
A
12.2


 


A-504




434.47
433.09 (M − H)−
A
9.22


 


A-505




427.45
426.07 (M − H)−
A
9.08


 


A-506




478.52
477.11 (M − H)−
A
8.04


 


A-507




471.5
470.11 (M − H)−
A
7.8


 


A-508




404.44
403.11 (M − H)−
A
7.86


 


A-509




395.43
394.09 (M − H)−
A
7.06


 


A-510




418.42
417.02 (M − H)−
A
7.55


 


A-511




411.4
410.03 (M − H)−
A
7.01


 


A-512




383.39
382.07 (M − H)−
A
7.48


 


A-513




397.42
396.1 (M − H)−
A
7.88


 


A-514




411.45
410.07 (M − H)−
A
8.24


 


A-515




423.48
422.03 (M − H)−
A
8.28


 


A-516




423.48
422.03 (M − H)−
A
8.23


 


A-517




418.47
417.02 (M − H)−
A
8.4


 


A-518




421.47
420.01 (M − H)−
A
8.07


 


A-519




421.47
420.01 (M − H)−
A
8


 


A-520




416.45
414.98 (M − H)−
A
8


 


A-521




409.43
407.99 (M − H)−
A
7.98


 


A-522




429.44
428.00 (M − H)−
A
8.73


 


A-523




395.43
394.02 (M − H)−
A
7.51


 


A-524




395.43
394.02 (M − H)−
A
7.44


 


A-525




395.43
394.02 (M − H)−
A
7.06


 


A-526




420.44
419.10 (M − H)−
A
7.66


 


A-527




404.44
403.12 (M − H)−
A
10.39


 


A-528




420.44
419.10 (M − H)−
A
7.6


 


A-529




404.44
403.12 (M − H)−
A
10.27


 


A-530




441.47
440.16 (M − H)−
A
6.98


 


A-531




387.33
NA
A
6.6


 


A-532




419.32
419.95
A
6.84


 


A-533




413.42
414.03
A
7.26


 


A-534




383.37
384.02
A
6.9


 


A-535




401.36
402.01
A
7.6


 


A-536




399.37
400
A
6.6


 


A-537




399.37
400
A
6.74


 


A-538




413.42
412.18 (M − H)−
A
7.28


 


A-539




409.46
410.07
A
7.83


 


A-540




409.46
410.07
A
8.24


 


A-541




469.82
469.92, 471.9
A
8.41


 


A-542




469.82
469.92, 471.9
A
9.07


 


A-543




469.82
469.92, 471.9 (M − H)−
A
9.17


 


A-544




353.34
352.17 (M − H)−
A
8.8


 


A-545




365.35
364.18 (M − H)−
A
7.04


 


A-546




397.37
396.17 (M − H)−
A
8.08


 


A-547




379.38
378.16 (M − H)−
A
7.44


 


A-548




383.34
382.19 (M − H)−
A
7.49


 


A-549




453.82
452.10, 454.11 (M − H)−
A
8.91


 


A-550




393.46
392.25 (M − H)−
A
8.27


 


A-551




453.82
452.15, 454.15 (M − H)−
A
9.56


 


A-552




393.46
392.26 (M − H)−
A
8.4


 


A-553




471.81
470.13, 472.12 (M − H)−
A
10.86


 


A-554




411.45
410.27 (M − H)−
A
10.05


 


A-555




471.81
470.10, 472.09 (M − H)−
A
10.39


 


A-556




411.45
410.21 (M − H)−
A
9.34


 


A-557




447.81
448
D
1.9


 


A-558




467.8
468
D
2.5


 


A-559




431.36
432
D
1.76


 


A-560




463.38
464
D
1.9


 


A-561




483.36
484
D
2.45


 


A-571




477.45
NA
NA
NA


 


A-572




493.45
NA
NA
NA


 


A-573




491.48
NA
NA
NA


 


A-574




489.47
NA
NA
NA


 


A-575




477.45
NA
NA
NA


 


A-576




441.52
NA
NA
NA


 


A-577




463.43
NA
NA
NA


 


A-578




479.43
NA
NA
NA


 


A-579




477.45
NA
NA
NA


 


A-580




475.44
NA
NA
NA


 


A-581




463.43
NA
NA
NA


 


A-582




463.43
NA
NA
NA


 


A-583




535.85
NA
NA
NA


 


A-584




477.45
NA
NA
NA


 


A-585




477.45
NA
NA
NA


















TABLE 3





Compound
NMR



No.
Solvent
1H NMR Resonance Data (δ)







A-4
DMSO-d6
14.21 (bs, 0.5H), 12.82 (bs, 0.5H), 12.73




(s, 0.5H), 10.02 (s, 0.5H), 8.67 (bs, 0.5H),




8.07 (bs, 1.5H), 7.80 (d, 1H), 7.57-7.51 (m, 2H), 7.37 (s, 0.5H),




6.82 (bs, 1H), 5.83 (s, 0.5H), 1.52 (s, 6H), 1.38 (s, 3H).


A-37
DMSO-d6
13.62 (s, 1H), 12.20 (bs, 0.3H), 8.59




(m, 1H), 8.38 (bs, 1H), 8.12 (d, 1H), 7.92 (d, 1H), 7.64 (d, 1H), 7.52




(d, 1H), 1.54 (s, 5H), 1.46 (s, 4H).


A-40
DMSO-d6
13.60 (br s, 1H), 8.55 (br s, 1H), 7.88 (m, 2H), 7.68 (m, 1H), 6.77 (m,




1H), 5.85 (br s, 1H), 1.5 (s, 6H).


A-41
DMSO-d6
13.40-12.40 (br m, 1H), 10.62-10.12 (br m, 1H), 9.38-8.60 (br m,




1H), 8.00-7.40 (br m, 3H), 6.85-6.40 (br m, 1H), 4.40-3.80 (br m,




1H), 1.95 (bs, 2H), (bm, 11H).


A-44
DMSO-d6
12.68 (bs, 1H), 8.55 (bs, 1H), 7.93 (m, 2H), 7.51 (q, 1H, J = 8.4 Hz),




6.86 (s, 1H), 3.90 (s, 3H), 1.53 (s, 9H).


A-45
DMSO-d6
12.61 (s, 1H), 12.54 (s, 1H), 11.03 (s, 1H), 7.89 (m, 2H), 7.66 (s, 1H),




7.55 (m, 2H), 7.24 (d, 1H, J = 8.8 Hz), 2.24 (s, 3H).


A-48
DMSO-d6
10.86 (s, 1H), 10.22 (s, 1H), 8.04 (d, 2H, J = 8.0 Hz), 7.93 (d, 2H,




J = 8.0 Hz), 7.65 (m, 2H), 7.11 (d, 1H, J = 8.4 Hz), 6.05 (s, 1H), 3.74 (s,




3H).


A-49
DMSO-d6
8.18 (d, 2H, J = 8 Hz), 7.90-7.79 (m, 4H), 7.49 (dd, 2H, J = 8.8, 5.2),




7.17 (t, 2H, J = 8.8 Hz), 5.56 (bs, 2H), 1.45 (s, 9H).


A-50
DMSO-d6
13.25 (s, 1H), 12.84 (s, 1H), 9.01 (s, 1H), 8.28 (d, 2H, J = 8.0 Hz),




7.83 (d, 2H, J = 8.0 Hz), 7.79 (dd, 2H, J = 8.8, 5.2 Hz), 7.32 (t, 2H,




J = 8.8 Hz), 6.60 (s, 1H), 1.56 (s, 9H).


A-53
DMSO-d6
13.59 (br s, 1H), 12.72 (s, 1H), 8.87 (s, 1H), 8.27 (d, 2H), 7.84 (d,




2H), 7.16 (t, 1H), 6.50 (s, 1H), 1.54 (s, 9H).


A-54
DMSO-d6
13.57 (br s, 1H), 12.78 (br s, 1H), 8.79 (br s, 1H), 8.08 (m, 2H), 7.57




(m, 2H), 7.15 (t, 1H), 6.48 (s, 1H), 1.52 (s, 9H).


A-55
DMSO-d6
13.52 (br s, 1H), 12.58 (br s, 1H), 8.54 (br s, 1H), 8.1 (m, 2H), 7.48




(m, 2H), 7.16 (t, 1H), 6.50 (s, 1H), 4.4 (m, 1H), 1.24 (m, 6H).


A-56
DMSO-d6
13.52 (br s, 1H), 12.55 (br s, 1H), 8.94 (br s, 1H), 8.07 (m, 2H), 7.62




(m, 1H), 7.5-7.35 (m, 4H), 6.54 (s, 1H), 4.75 (br m, 2H).


A-58
DMSO-d6
13.55 (br s, 1H), 12.52 (br s, 1H), 8.6 (br s, 1H), 8.29 (m, 2H), 7.79




(m, 2H), 7.16 (t, 1H), 6.52 (s, 1H), 4.41 (m, 1H), 1.26 (m, 6H).


A-62
DMSO-d6
13.58 (br s, 1H), 12.65 (br s, 1H), 8.84 (s, 1H), 7.95 (m, 2H), 7.53 (m,




1H), 7.15 (t, 1H), 6.48 (s, 1H), 1.52 (s, 9H).


A-63
DMSO-d6
13.55 (br s, 1H), 12.46 (br s, 1H), 8.6 (br s, 1H), 8.01 (m, 2H), 7.48




(m, 1H), 7.16 (t, 1H), 6.50 (s, 1H), 4.39 (m, 1H), 1.25 (m, 6H).


A-65
DMSO-d6
13.6 (br s, 1H), 12.63 (br s, 1H), 8.86 (br s, 1H), 7.91 (m, 2H), 7.71




(m, 1H), 7.16 (t, 1H), 6.49 (s, 1H), 1.52 (s, 9H).


A-69
DMSO-d6
13.6 (br s, 1H), 12.64 (br s, 1H), 8.87 (br s, 1H), 8.10 (m, 2H), 7.48




(m, 2H), 7.16 (t, 1H), 6.51 (s, 1H), 3.44 (m, 2H), 0.96 (s, 9H).


A-125
DMSO-d6
12.59 (s, 1H), 12.45 (d, J = 1 Hz, 1H), 9.04 (s, 1H), 8.24 (d, J = 8 Hz,




2H), 7.80 (d, J = 8 Hz, 2H), 5 84 (d, J = 8 Hz, 1H), 1.51 (s, 9H).


A-127
DMSO-d6
13.24 (s, 1H), 12.59 (s, 1H), 8.82 (d, J = 6.7 Hz, 1H), 8.41 (m, 2H),




7.96 (d, J = 7.6 Hz, 1H), 7.76 (m, 2H), 7.67, (t, J = 7.6 Hz, 1H), 7.32




(t, J = 8.6 Hz, 2H), 6.61 (s, 1H), 4.44 (q, J = 6.7 Hz, 1H), 1.29 (d, J =




6.3 Hz, 6H).


A-155
DMSO-d6
12.54 (s, 1H), 12.50 (s, 1H), 9.18 (t, 1H), 8.30 (d, 2H), 7.80 (d, 2H),




5.93 (s, 1H) 3.47 (d, 2H), 2.23 (s, 3H), 0.98 (s, 3H).


A-158
DMSO-d6
13.18 (s, 1H), 12.58 (s, 1H), 8.95 (d, 1H), 8.00 (m, 2H), 7.70 (d, 2H),




7.49 (m, 1H), 7.40 (d, 2H), 6.57 (s, 1H), 5.22 (m, 1H), 4.53 (m, 3H),




2.05 (m, 2H), 1.60 (m, 6H).


A-159
DMSO-d6
13.20 (s, 1H), 12.78 (s, 1H), 9.00 (s, 1H), 7.96 (m, 2H), 7.70 (d, 2H),




7.53 (m, 1H), 7.40 (d, 2H), 6.58 (s, 1H), 5.23 (t, 1H), 4.50 (d, 2H),




1.55 (s, 9H).


A-160
DMSO-d6
13.32 (s, 1H), 12.65 (s, 1H), 8.22 (t, 1H), 8.00 (m, 2H), 7.70 (d, 2H),




7.60 (d, 2H), 7.50 (m, 1H), 3.62 (m, 2H), 3.42 (m, 5H), 1.81 (m, 2H),




1.10 (m, 3H).


A-162
DMSO-d6
13.41 (s, 1H), 13.15 (s, 1H), 8.44 (s, 1H), 7.97 (m, 2H), 7.70 (d, 2H),




7.60 (d, 2H), 7.55 (m, 1H), 1.54 (s, 9H).


A-163
DMSO-d6
13.11 (s, 1H), 12.80 (s, 1H), 8.86 (s, 1H), 8.75 (t, 1H), 8.14 (m, 1H),




7.79 (t, 2H), 7.30 (t, 2H), 6.80 (d, 1H), 6.56 (s, 1H), 3.65 (m, 4H),




3.52 (m, 4H), 3.40 (m, 2H), 1.92 (m, 1H), 0.95 (d, 6H).


A-164
DMSO-d6
13.20 (s, 1H), 12.97 (s, 1H), 8.82 (s, 1H), 8.79 (s, 1H), 8.10 (d, 1H),




7.79 (t, 2H), 7.31 (t, 2H), 6.82 (d, 1H), 6.52 (s, 1H), 3.98 (m, 1H),




3.65 (m, 4H), 3.55 (m, 4H), 1.55 (s, 9H).


A-171
DMSO-d6
13.18 (s, 1H), 12.80 (s, 1H), 8.84 (t, 1H), 7.80-7.65 (m, 4H), 7.32




(m, 3H), 7.03 (m, 1H), 6.59 (s, 1H), 3.78 (s, 3H), 3.42 (m, 2 H), 1.95




(m, 1H), 0.96 (d, 6H).


A-173
DMSO-d6
13.08 (s, 1H), 10.46 (s, 1H), 9.72 (s, 1H), 7.95 (t, 2H), 7.65 (d, 2H),




7.40 (t, 2H), 7.20 (d, 2H), 5.95 (s, 1H), 3.60 (m, 5H).


A-174
DMSO-d6
13.23 (s, 1H), 12.60 (s, 1H), 8.82 (d, 1H), 8.58 (d, 1H), 7.78 (m, 4H),




7.33 (t, 2H), 6.60 (s, 1H), 4.30 (m, 1H), 2.80 (m, 2H), 1.62 (m, 2H),




1.24 (m, 6H), 0.95 (m, 3H).


A-176
DMSO-d6
13.20 (s, 1H), 12.59 (s, 1H), 8.98 (t, 1H), 8.58 (d, 1H), 7.80 (m, 4H),




7.33 (t, 2H), 6.61 (s, 1H), 3.45 (m, 2H), 2.80 (m, 2H), 1.93 (m, 1H),




1.22 (t, 3H), 0.98 (d, 6H).


A-177
DMSO-d6
13.25 (s, 1H), 12.75 (s, 1H), 9.02 (s, 1H), 8.60 (s, 1H), 7.79 (m, 4H),




7.31 (t, 2H), 6.60 (s, 1H), 3.15 (s, 2H), 2.81 (q, 2H), 1.57 (s, 9H),




1.23 (t, 3H).


A-206
DMSO-d6
13.22 (s, 1H), 12.78 (s, 1H), 8.75 (d, 1H), 8.19 (t, 2H), 7.79 (t, 2H),




7.34 (t, 2H), 7.22 (t, 2H), 6.60 (s, 1H), 4.27 (m, 1H), 1.60 (m, 2H),




1.23 (d, 3H), 0.95 (t, 3H).


A-209
DMSO-d6
13.28 (s, 1H), 12.78 (s, 1H), 8.72 (d, 1H), 8.19 (t, 2H), 7.78 (d, 2H),




7.55 (d, 2H), 7.23 (t, 2H), 6.63 (s, 1H), 4.28 (m, 1H), 1.60 (m, 2H),




1.23 (d, 3H), 0.94 (t, 3H).


A-213
DMSO-d6
13.15 (s, 1H), 12.61 (s, 1H), 9.06 (t, 1H), 7.98 (t, 2H), 7.80 (t, 2H),




7.64 (t, 1H), 7.33 (t, 2H), 6.60 (s, 1H), 3.81 (m, 1H), 3.66 (m, 1H),




3.50 (m, 3H), 1.13 (m, 6H).


A-214
DMSO-d6
13.20 (s, 1H), 12.63 (s, 1H), 9.00 (t, 1H), 8.00 (m, 2H), 7.78 (d, 2H),




7.50 (m, 3H), 6.62 (s, 1H), 3.82 (m, 1H), 3.65 (m, 1H), 3.50 (m, 3H),




1.15 (m, 6H).


A-216
DMSO-d6
13.22 (s, 1H), 12.58 (s, 1H), 8.80 (d, 1H), 7.95 (d, 2H), 7.79 (t, 2H),




7.64 (m, 1H), 7.33 (t, 2H), 6.60 (s, 1H), 4.40 (septet, 1H), 1.27 (d,




6H).


A-218
DMSO-d6
13.22 (s, 1H), 12.61 (s, 1H), 9.00 (d, 1H), 7.97 (m, 2H), 7.78 (t, 2H),




7.65 (m, 1H), 7.30 (t, 2H), 6.60 (s, 1H), 4.55 (m, 1H), 3.46 (m, 2H),




3.30 (s, 3H), 3.12 (s, 1H), 1.22 (d, 3H).


A-219
DMSO-d6
13.22 (s, 1H), 12.60 (s, 1H), 8.80 (d, 1H), 7.94 (m, 2H), 7.80 (t, 2H),




7.63 (t, 1H), 7.32 (t, 2H), 6.60 (s, 1H), 4.27 (m, 1H), 1.60 (m, 2H),




1.22 (d, 3H), 0.93 (t, 3H).


A-220
DMSO-d6
13.20 (s, 1H), 12.59 (s, 1H), 8.95 (t, 1H), 7.95 (d, 2H), 7.80 (t, 2H),




7.65 (t, 1H), 7.32 (t, 2H), 6.60 (s, 1H), 3.23 (m, 2H), 1.92 (m, 1H),




0.97 (d, 6H).


A-221
DMSO-d6
13.21 (s, 1H), 12.57 (s, 1H), 8.96 (d, 1H), 7.97 (m, 2H), 7.80 (t, 2H),




7.64 (t, 1H), 7.32 (t, 2H), 6.60 (s, 1H), 4.54 (m, 1H), 2.05 (m, 2H),




1.60 (m, 6H).


A-222
DMSO-d6
13.22 (s, 1H), 12.52 (s, 1H), 9.02 (d, 1H), 7.96 (m, 2H), 7.80 (t, 2H),




7.65 (m, 1H), 7.33 (t, 2H), 6.60 (s, 1H), 4.62 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.75 (m, 2H).


A-225
DMSO-d6
13.30 (s, 1H), 12.62 (s, 1H), 8.77 (d, 1H), 7.99 (m, 2H), 7.73 (d, 2H),




7.50 (m, 3H), 6.62 (s, 1H), 4.28 (m, 1H), 1.60 (m, 2H), 1.23 (d, 3H),




0.94 (t, 3H).


A-230
DMSO-d6
13.40 (s, 1H), 12.75 (s, 1H), 8.62 (d, 1H), 8.11 (d, 2H), 7.49 (m, 4H),




7.27 (t, 1H), 6.75 (s, 1H), 4.40 (m, 1H), 1.27 (d, 6H).


A-231
DMSO-d6
13.40 (s, 1H), 12.70 (s, 1H), 8.86 (d, 1H), 8.13 (d, 2H), 7.50 (m, 4H),




7.28 (t, 1H), 6.78 (s, 1H), 4.62 (m, 1H), 2.40 (m, 2H), 2.00 (m, 2H),




1.75 (m, 2H).


A-233
DMSO-d6
13.38 (s, 1H), 12.78 (s, 1H), 8.80 (t, 1H), 8.10 (d, 2H), 7.50 (m, 4H),




7.29 (t, 1H), 6.77 (s, 1H), 3.43 (m, 2H), 1.93 (m, 1H), 0.96 (d, 6H).


A-234
DMSO-d6
13.40 (s, 1H), 12.79 (s, 1H), 8.65 (d, 1H), 8.10 (d, 2H), 7.50 (m, 4H),




7.29 (t, 1H), 6.78 (s, 1H), 4.27 (m, 1H), 1.60 (m, 2H), 1.22 (d, 3H),




0.93 (t, 3H).


A-235
DMSO-d6
13.15 (s, 1H), 12.61 (s, 1H), 9.00 (t, 1H), 8.00 (m, 2H), 7.80 (m, 2H),




7.47 (m, 1H), 7.32 (t, 2H), 6.60 (s, 1H), 3.78 (m, 2H), 3.60 (m, 2H),




3.48 (m, 2H), 1.12 (t, 3H).


A-240
DMSO-d6
13.26 (s, 1H), 12.64 (s, 1H), 8.93 (t, 1H), 8.30 (d, 2H), 7.80 (m, 4H),




7.57 (d, 2H), 6.65 (s, 1H), 3.46 (t, 2H), 1.93 (m, 1H), 0.98 (d, 6H).


A-241
DMSO-d6
13.30 (s, 1H), 12.63 (s, 1H), 8.78 (d, 1H), 8.29 (d, 2H), 7.78 (m, 4H),




7.55 (d, 2H), 6.63 (s, 1H), 4.42 (m, 1H), 1.30 (d, 6H).


A-242
DMSO-d6
13.33 (s, 1H), 12.81 (s, 1H), 8.82 (d, 1H), 8.18 (m, 2H), 7.52 (m, 2H),




7.25 (m, 3H), 6.75 (s, 1H), 4.52 (m, 1H), 3.51 (m, 4H), 1.28 (d, 3H),




1.13 (t, 3H).


A-243
DMSO-d6
13.38 (s, 1H), 12.78 (s, 1H), 8.80 (d, 1H), 8.20 (t, 2H), 7.50 (t, 2H),




7.22 (m, 3H), 6.75 (s, 1H), 4.52 (m, 1H), 2.03 (m, 2H), 1.60 (m, 6H).


A-244
DMSO-d6
13.33 (s, 1H), 12.80 (s, 1H), 8.78 (t, 1H), 8.18 (t, 2H), 7.52 (t, 2H),




7.22 (m, 3H), 6.77 (s, 1H), 3.43 (m, 2H), 1.91 (m, 1H), 0.97 (d, 6H).


A-245
DMSO-d6
13.38 (s, 1H), 12.80 (s, 1H), 8.60 (d, 1H), 8.18 (t, 2H), 7.50 (t, 2H),




7.25 (m, 3H), 6.74 (s, 1H), 4.40 (m, 1H), 1.27 (d, 6H).


A-246
DMSO-d6
13.40 (s, 1H), 12.69 (s, 1H), 8.88 (d, 1H), 8.00 (m, 2H), 7.72 (s, 1H),




7.62 (d, 1H), 7.47 (m, 2H), 6.78 (s, 1H), 4.55 (m, 1H), 3.48 (d, 2H),




3.30 (s, 3H), 1.25 (d, 3H).


A-249
DMSO-d6
13.19 (s, 1H), 12.71 (s, 1H), 8.89 (t, 1H), 8.11 (d, 2H), 7.80 (t, 2H),




7.48 (d, 2H), 7.32 (t, 2H), 6.60 (s, 1H), 3.42 (t, 2H), 1.92 (m, 1H),




0.98 (d, 6H).


A-250
DMSO-d6
13.21 (s, 1H), 12.70 (s, 1H), 8.72 (d, 1H), 8.12 (d, 2H), 7.78 (t, 2H),




7.47 (d, 2H), 7.31 (t, 2H), 6.60 (s, 1H), 4.40 (m, 1H), 1.27 (d, 6H).


A-254
DMSO-d6
13.24 (s, 1H), 12.75 (s, 1H), 8.83 (t, 1H), 8.19 (t, 2), 7.77(d, 2H), 7.54




(d, 2H), 7.23 (t, 2H), 6.63 (s, 1H), 3.44 (t, 2H), 1.93 (m, 1H), 0.98 (d,




6H).


A-255
DMSO-d6
13.25 (s, 1H), 12.75 (s, 1H), 8.67 (d, 1H), 8.18 (t, 2H), 7.74 (d, 2H),




7.53 (d, 2H), 7.23 (t, 2H), 6.62 (s, 1H), 4.41 (m, 1H), 1.27 (d, 6H).


A-256
DMSO-d6
13.19 (s, 1H), 12.70 (s, 1H), 8.96 (d, 1H), 7.95 (d, 1H), 7.80 (m, 3H),




7.47 (t, 1H), 7.32 (m, 3H), 6.60 (s, 1H), 4.51 (m, 1H), 3.50 (m, 4H),




1.27 (d, 3H), 1.14 (t, 3H).


A-257
DMSO-d6
13.20 (s, 1H), 12.67 (s, 1H), 8.90 (t, 1H), 7.98 (d, 1H), 7.80 (m, 3H),




7.48 (m, 1H), 7.33 (m, 3H), 6.60 (s, 1H), 3.44 (m, 2H), 1.93 (m, 1H),




0.98 (d, 6H).


A-258
DMSO-d6
13.23 (s, 1H), 12.62 (s, 1H), 8.97 (d, (1H), 8.00 (m, 2H), 7.75 (d, 2H),




7.50 (m, 3H), 6.62 (s, 1H), 4.51 (m, 1H), 3.51 (m, 4H), 1.26 (d, 3H),




1.13 (t, 3H).


A-259
DMSO-d6
13.22 (s, 1H), 12.53 (s, 1H), 9.00 (d, 1H), 8.00 (m, 2H), 7.78 (m, 2H),




7.48 (m, 1H), 7.30 (m, 2H), 6.60 (s, 1H), 4.64 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.78 (m, 2H).


A-260
DMSO-d6
13.20 (s, 1H), 12.59 (s, 1H), 8.95 (d, 1H), 8.00 (m, 2H), 7.77 (m, 2H),




7.48 (m, 1H), 7.30 (t, 2H). 6.59 (s, 1H), 4.55 (m, 1H), 2.05 (m, 2H),




1.60 (m, 6H).


A-261
DMSO-d6
13.20 (s, 1H), 12.60 (s, 1H), 8.92 (t, 1H), 7.98 (m, 2H), 7.78 (m, 2H),




7.47 (m, 1H), 7.30 (t, 2H), 6.60 (s, 1H), 3.43 (t, 2H), 1.92 (m, 1H),




0.95 (d, 6H).


A-263
DMSO-d6
13.19 (s, 1H), 12.62 (s, 1H), 8.99 (d, 1H), 8.00 (m, 2H), 7.78 (m, 2H),




7.48 (m, 1H), 7.33 (t, 2H), 6.60 (s, 1H), 4.51 (m, 1H), 3.50 (m, 4H),




1.26 (d, 3H), 1.12 (t, 3H).


A-264
DMSO-d6
13.21 (s, 1H), 12.64 (s, 1H), 8.98 (d, 1H), 8.12 (d, 2H), 7.78 (t, 2H),




7.48 (d, 2H), 7.30 (t, 2H), 6.60 (s, 1H), 4.63 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.75 (m, 2H).


A-267
DMSO-d6
13.18 (s, 1H), 12.62 (s, 1H), 8.98 (d, 1H), 8.00 (m, 2H), 7.79 (t, 2H),




7.48 (m, 1H), 7.33 (t, 2H), 6.59 (s, 1H), 4.52 (m, 1H), 3.50 (m, 4H),




1.26 (d, 3H), 1.14 (t, 3H).


A-268
DMSO-d6
13.22 (s, 1H), 12.65 (s, 1H), 8.97 (d, 1H), 8.00 (m, 2H), 7.78 (t, 2H),




7.49 (m, 1H), 7.31 (t, 2H), 6.60 (s, 1H), 4.42 (m, 1H), 3.48 (m, 2H),




3.30 (s, 3H), 1.63 (m, 2H), 0.94 (t, 3H).


A-274
DMSO-d6
13.22 (s, 1H), 12.63 (s, 1H), 8.98 (d, 1H), 7.99 (m, 2H), 7.78 (t, 2H),




7.47 (m, 1H), 7.32 (t, 2H), 6.60 (s, 1H), 4.55 (m, 1H), 3.47 (d, 2H),




3.30 (s, 3H), 1.25 (d, 3H).


A-282
DMSO-d6
13.31 (s, 1H), 12.67 (s, 1H), 8.72 (t, 1H), 8.30 (d, 2H), 7.78 (d, 2H),




7.52 (t, 2H), 7.28 (m, 1H), 6.80 (s, 1H), 3.65 (m, 2H), 3.42 (m, 4H),




1.82 (m, 2H), 1.07 (t, 3H).


A-283
DMSO-d6
13.26 (s, 1H), 12.60 (s, 1H), 8.84 (t, 1H), 8.00 (m, 2H), 7.73 (s, 1H),




7.62 (d, 1H), 7.44 (m, 2H), 6.76 (s, 1H), 3.62 (m, 2H), 3.48 (m, 3H),




1.80 (m, 2H), 1.03 (d, 6H).


A-284
DMSO-d6
13.40 (s, 1H), 12.59 (s, 1H), 8.88 (d, 1H), 8.00 (m, 2H), 7.72 (s, 1H),




7.60 (d, 1H), 7.47 (m, 2H), 6.80 (s, 1H), 4.63 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.76 (m, 2H).


A-285
DMSO-d6
13.40 (s, 1H), 12.64 (s, 1H), 8.68 (d, 1H), 7.98 (m, 2H), 7.70 (s, 1H),




7.60 (d, 1H), 7.48 (m, 2H), 6.78 (s, 1H), 4.28 (m, 1H), 1.60 (m, 2H),




1.23 (d, 3H), 0.95 (t, 3H).


A-286
DMSO-d6
13.40 (s, 1H), 12.62 (s, 1H), 8.63 (d, 1H), 7.99 (m, 2H), 7.70 (s, 1H),




7.61 (d, 1H), 7.48 (m, 2H), 6.77 (s, 1H), 4.40 (m, 1H), 1.27 (d, 6H).


A-287
DMSO-d6
13.38 (s, 1H), 12.62 (s, 1H), 8.80 (t, 1H), 8.00 (m, 2H), 7.72 (s, 1H),




7.61 (d, 1H), 7.48 (m, 2H), 6.80 (s, 1H), 3.45 (t, 2H), 1.92 (m, 1H),




0.97 (d, 6H).


A-288
DMSO-d6
13.40 (s, 1H), 12.79 (s, 1H), 8.95 (s, 1H), 7.97 (m, 2H), 7.72 (s, 1H),




7.61 (d, 1H), 7.48 (m, 2H), 6.78 (s, 1H), 2.19 (m, 2H), 1.75 (m, 6H),




1.60 (s, 3H).


A-289
DMSO-d6
13.38 (s, 1H), 12.61 (s, 1H), 8.81 (d, 1H), 8.00 (m, 2H), 7.70 (s, 1H),




7.61 (d, 1H), 7.45 (m, 2H), 6.78 (s, 1H), 4.52 (m, 1H), 2.04 (m, 2H),




1.60 (m, 6H).


A-290
DMSO-d6
13.41 (s, 1H), 12.82 (s, 1H), 8.90 (s, 1H), 7.96 (m, 2H), 7.73 (s, 1H),




7.62 (d, 1H), 7.50 (m, 2H), 6.76 (s, 1H), 4.06 (m, 1H), 3.12 (d, 3H),




1.53 (s, 9H).


A-291
DMSO-d6
13.40 (bs, 1H), 12.68 (s, 1H), 8.87 (d, 1H), 8.30 (d, 2H), 7.78 (d, 2H),




7.50 (d, 2H), 7.27 (t, 1H), 6.78 (s, 1H), 4.55 (m, 1H), 2.06 (m, 2H),




1.60 (m, 6H).


A-292
DMSO-d6
13.39 (s, 1H), 12.64 (s, 1H), 9.18 (t, 1H), 8.25 (d, 2H), 7.79 (d, 2H),




7.52 (m, 4H), 7.24 (m, 2H), 6.82 (s, 1H), 4.85 (d, 2H).


A-293
DMSO-d6
13.40 (s, 1H), 12.70 (s, 1H), 8.85 (t, 1H), 8.30 (d, 2H), 7.80 (d, 2H),




7.53 (d, 2H), 7.28 (t, 1H), 6.79 (s, 1H), 3.45 (t, 2H), 1.95 (m, 1H),




0.97 (d, 6H).


A-296
DMSO-d6
13.41 (s, 1H), 12.70 (s, 1H), 8.69 (d, 1H), 8.30 (d, 2H), 7.80 (d, 2H),




7.50 (d, 2H), 7.28 (t, 1H), 6.78 (s, 1H), 4.40 (m, 1H), 1.27 (d, 6H).


A-297
DMSO-d6
13.41 (s, 1H), 12.73 (s, 1H), 8.72 (d, 1H), 8.28 (d, 2H), 7.79 (d, 2H),




7.51 (d, 2H), 7.28 (t, 1H), 6.78 (s, 1H), 4.30 (m, 1H), 1.61 (m, 2H),




1.23 (d, 3H), 0.95 (t, 3H).


A-298
DMSO-d6
13.40 (s, 1H), 12.63 (s, 1H), 8.93 (d, 1H), 8.30 (d, 2H), 7.80 (d, 2H),




7.50 (d, 2H), 7.28 (t, 1H), 6.80 (s, 1H), 4.63 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.75 (m, 2H).


A-299
DMSO-d6
13.42 (s, 1H), 12.90 (s, 1H), 8.92 (s, 1H), 8.28 (d, 2H), 7.82 (d, 2H),




7.53 (d, 2H), 7.28 (t, 1H), 6.78 (s, 1H), 1.55 (s, 9H).


A-300
DMSO-d6
13.26 (s, 1H), 12.60 (s, 1H), 8.95 (t, 1H), 8.40 (m, 2H), 7.96 (d, 1H),




7.78 (d, 2H), 7.65 (t, 1H), 7.55 (d, 2H), 6.63 (s, 1H), 3.60 (m, 1H),




3.41 (m, 1H), 1.71 (m, 1H), 1.43 (m, 1H), 1.22 (m, 1H), 0.94 (m, 6H).


A-301
DMSO-d6
13.21 (s, 1H), 12.56 (s, 1H), 8.79 (t, 1H), 8.41 (m, 2H), 7.95 (d, 1H),




7.78 (d, 2H), 7.64 (t, 1H), 7.54 (d, 2H), 6.63 (s, 1H), 3.65 (m, 2H),




3.48 (m, 3H), 1.80 (m, 2H), 1.05 (d, 6H).


A-302
DMSO-d6
13.29 (s, 1H), 12.58 (s, 1H), 8.97 (d, 1H), 8.41 (s, 2H), 7.95 (d, 1H),




7.76 (d, 2H), 7.65 (d, 1H), 7.52 (d, 2H), 6.63 (s, 1H), 4.57 (m, 1H),




2.06 (m, 2H), 1.62 (m, 6H).


A-303
DMSO-d6
13.28 (s, 1H), 12.60 (s, 1H), 8.94 (t, 1H), 8.40 (m, 2H), 7.95 (d, 1H),




7.77 (d, 2H), 7.65 (t, 1H), 7.54 (d, 2H), 6.65 (s, 1H), 3.45 (t, 2H),




1.93 (m, 1H), 0.98 (d, 6H).


A-304
DMSO-d6
13.30 (s, 1H), 12.62 (s, 1H), 8.94 (d, 1H), 8.40 (m, 2H), 7.95 (d, 1H),




7.77 (d, 2H), 7.65 (t, 1H), 7.53 (d, 2H), 6.64 (s, 1H), 4.30 (m, 1H),




1.90 (m, 1H), 1.20 (d, 3H), 0.95 (m, 6H).


A-305
DMSO-d6
13.30 (s, 1H), 12.59 (s, 1H), 8.80 (d, 1H), 8.40 (m, 2H), 7.97 (d, 1H),




7.76 (d, 2H), 7.66 (t, 1H), 7.54 (d, 2H), 6.63 (s, 1H), 4.42 (m, 1H),




1.27 (d, 6H).


A-306
DMSO-d6
13.31 (s, 1H), 12.61 (s, 1H), 8.80 (d, 1H), 8.40 (m, 2H), 7.96 (d, 1H),




7.76 (d, 2H), 7.65 (t, 1H), 7.53 (d, 2H), 6.64 (s, 1H), 4.31 (m, 1H),




1.62 (m, 2H), 1.23 (d, 3H), 0.96 (t, 3H).


A-307
DMSO-d6
13.32 (s, 1H), 12.53 (s, 1H), 9.02 (d, 1H), 8.40 (m, 2H), 7.95 (d, 1H),




7.75 (d, 2H), 7.65 (t, 1H), 7.52 (d, 2H), 6.64 (s, 1H), 4.65 (m, 1H),




2.40 (m, 2H), 2.00 (m, 2H), 1.75 (m, 2H).


A-308
DMSO-d6
13.32 (s, 1H), 12.78 (s, 1H), 9.00 (s, 1H), 8.40 (m, 2H), 7.98 (d, 1H),




7.78 (d, 2H), 7.70 (t, 1H), 7.53 (d, 2H), 6.63 (s, 1H), 1.54 (s, 9H).


A-309
DMSO-d6
13.40 (bs, 1H), 12.62 (s, 1H), 8.89 (d, 1H), 8.40 (m, 2H), 7.95 (d,




1H), 7.66 (t, 1H), 7.50 (d, 2H), 7.28 (t, 1H), 6.78 (s, 1H), 4.58 (m,




1H), 2.05 (m, 2H), 1.60 (m, 6H).


A-310
DMSO-d6
13.39 (bs, 1H), 12.62 (s, 1H), 8.85 (t, 1H), 8.40 (m, 2H), 7.96 (d, 1H),




7.65 (t, 1H), 7.52 (d, 2H), 7.26 (t, 1H), 6.79 (s, 1H), 3.45 (t, 2H), 1.92




(m, 1H), 0.95 (d, 6H).


A-311
DMSO-d6
13.42 (s, 1H), 12.69 (s, 1H), 8.83 (d, 1H), 8.40 (m, 2H), 7.98 (d, 1H),




7.65 (d, 1H), 7.52 (d, 2H), 7.28 (t, 1H), 6.79 (s, 1H), 4.31 (m, 1H),




1.90 (m, 1H), 1.20 (d, 1H), 0.95 (m, 6H).


A-313
DMSO-d6
13.40 (s, 1H), 12.63 (s, 1H), 8.73 (d, 1H), 8.40 (m, 2H), 7.97 (d, 1H),




7.64 (t, 1H), 7.50 (d, 2H), 7.26 (t, 1H), 6.78 (s, 1H), 4.30 (m, 1H),




1.60 (m, 2H), 1.23 (d, 3H), 0.94 (t, 3H).


A-314
DMSO-d6
13.42 (bs, 1H), 12.59 (s, 1H), 8.96 (d, 1H), 8.40 (m, 2H), 7.96 (d,




1H), 7.65 (t, 1H), 7.50 (d, 2H), 7.29 (t, 1H), 6.79 (s, 1H), 4.66 (m,




1H), 2.40 (m, 2H), 2.00 (m, 2H), 1.75 (m, 2H).


A-319
DMSO-d6
13.24 (s, 1H), 12.60 (s, 1H), 8.78 (d, 1H), 8.00 (m, 2H), 7.53 (m, 3H),




7.40 (m, 1H), 6.63 (s, 1H), 4.41 (m, 1H), 3.14 (s, 2H), 2.22 (s, 3H),




1.26 (d, 6H).


A-323
DMSO-d6
13.22 (s, 1H), 12.62 (s, 1H), 8.90 (t, 1H), 8.00 (m, 2H), 7.52 (m, 3H),




7.40 (m, 1H), 6.63 (s, 1H), 3.45 (t, 2H), 2.22 (s, 3H), 1.93 (m, 1H),




0.98 (d, 6H).


A-335
DMSO-d6
12.80 (s, 1H), 12.53 (s, 1H), 8.80 (t, 1H), 8.28 (d, 2H), 7.80 (d, 2H),




7.52 (m, 2H), 7.22 (t, 1H), 4.84 (d, 2H), 1.30 (s, 9 H).


A-338
DMSO-d6
14.0 (s, 1H), 12.46 (s, 1H), 9.01 (t, 1H), 8.23 (d, 2H), 7.79 (d, 2H),




7.50 (m, 2H), 7.21 (m, 1H), 6.80 (s, 1H), 4.82 (d, 2H), 3.82 (s, 3H).


A-339
DMSO-d6
13.93 (s, 1H), 12.69 (s, 1H), 8.80 (s, 1H), 8.28 (d, 2H), 7.83 (d, 2H),




6.74 (s, 1H), 3.82 (s, 3H), 1.53 (s, 9H).


A-340
DMSO-d6
13.08 (s, 1H), 12.58 (s, 1H), 8.60 (s, 1H), 8.48 (d, 2H), 7.82 (d, 2H),




1.57 (s, 9H), 1.30 (s, 9H).


A-346
DMSO-d6
13.39 (s, 1H), 12.62 (s, 1H), 8.83 (s, 1H), 7.99 (d, 2H), 7.69 (d, 1H),




7.53 (d, 2H), 7.30 (t, 1H), 6.80 (s, 1H), 3.60 (m, 1H), 3.45 (m, 1H),




1.70 (m, 1H), 1.45 (m, 1H), 0.94 (m, 6H).


A-347
DMSO-d6
13.42 (bs, 1H), 12.59 (s, 1H), 8.94 (d, 1H), 7.99 (d, 2H), 7.66 (d, 1H),




7.53 (d, 2H), 7.28 (t, 1H), 6.80 (s, 1H), 4.65 (m, 1H), 2.40 (m, 2H),




2.00 (m, 2H), 1.78 (m, 2H).


A-351
DMSO-d6
13.42 (s, 1H), 12.70 (s, 1H), 8.82 (d, 1H), 7.98 (d, 2H), 7.69 (t, 1H),




7.52 (d, 2H), 7.30 (t, 1H), 6.79 (s, 1H), 4.30 (t, 1H), 1.90 (m, 1H),




1.20 (d, 3H), 0.99 (m, 6H).


A-352
DMSO-d6
13.38 (s, 1H), 12.62 (s, 1H), 8.84 (t, 1H), 7.95 (d, 2H), 7.65 (m, 1H),




7.53 (m, 2H), 7.28 (t, 1H), 6.79 (s, 1H), 3.44 (m, 2H), 1.92 (m, 1H),




0.96 (d, 6H).


A-356
DMSO-d6
13.41 (s, 1H), 12.63 (s, 1H), 8.71 (d, 1H), 7.95 (m, 2H), 7.66 (m, 1H),




7.52 (m, 2H), 7.28 (t, 1H), 6.78 (s, 1H), 4.28 (m, 1H), 1.60 (m, 2H),




1.22 (d, 3H), 0.98 (t, 3H).


A-362
DMSO-d6
13.41 (s, 1H), 12.63 (s, 1H), 8.70 (d, 1H), 7.94 (m, 2H), 7.65 (m, 1H),




7.52 (d, 2H), 7.28 (t, 1H), 6.77 (s, 1H), 4.28 (m, 1H), 1.60 (m, 2H),




1.22 (d, 3H), 0.93 (t, 3H).


A-363
DMSO-d6
13.40 (s, 1H), 12.69 (s, 1H), 8.88 (d, 1H), 8.00 (m, 2H), 7.50 (m, 3H),




7.28 (t, 1H), 6.75 (s, 1H), 4.55 (m, 1H), 3.47 (d, 2H), 3.30 (s, 3H),




1.26 (d, 3H).


A-364
DMSO-d6
13.38 (s, 1H), 12.64 (s, 1H), 8.81 (t, 1H), 8.00 (m, 2H), 7.52 (m, 3H),




7.28 (t, 1H), 6.78 (s, 1H), 3.45 (m, 2H), 1.92 (m, 1H), 0.97 (d, 6H).


A-365
DMSO-d6
13.40 (s, 1H), 12.63 (s, 1H), 8.82 (d, 1H), 8.00 (m, 2H), 7.50 (m, 3H),




7.28 (t, 1H), 6.75 (s, 1H), 4.53 (t, 1H), 4.07 (m, 1H), 3.14 (m, 2H),




2.06 (m, 2H), 1.61 (m, 6H).


A-366
DMSO-d6
13.40 (s, 1H), 12.63 (s, 1H), 8.66 (d, 1H), 8.00 (m, 2H), 7.50 (m, 3H),




7.28 (t, 1H), 6.74 (s, 1H), 4.40 (m, 1H), 4.07 (m, 1H), 3.14 (m, 4H),




1.25 (d, 6H).


A-369
DMSO-d6
13.40 (s, 1H), 12.72 (s, 1H), 8.81 (d, 1H), 8.00 (d, 1H), 7.80 (d, 1H),




7.50 (m, 3H), 7.30 (m, 2H), 6.76 (s, 1H), 4.53 (m, 1H), 2.04 (m, 2H),




1.60 (m, 6H).


A-370
DMSO-d6
13.40 (s, 1H), 12.74 (s, 1H), 8.65 (d, 1H), 7.98 (d, 1H), 7.80 (d, 1H),




7.50 (m, 3H), 7.30 (m, 2H), 6.78 (s, 1H), 4.40 (m, 1H), 1.30 (d, 6H).


A-375
DMSO-d6
13.24 (s, 1H), 12.84 (s, 1H), 8.98 (s, 1H), 8.21 (d, 2H), 7.83 (d, 2H),




7.80 (t, 2H), 7.30 (t, 2H), 6.60 (s, 1H), 3.13 (s, 3H), 2.89 (s, 3H), 1.55




(s, 9H).


A-376
DMSO-d6
13.30 (s, 1H), 12.63 (s, 1H), 8.94 (d, (1H), 7.98 (m, 2H), 7.74 (d, 2H),




7.50 (m, 3H), 6.62 (s, 1H), 4.55 (m, 1H), 4.05 (t, 1H), 3.47 (s, 2H),




3.30 (s, 3H), 3.12 (d, 2H), 1.23 (d, 3H).


A-377
DMSO-d6
13.27 (s, 1H), 12.59 (s, 1H), 8.89 (t, 1H), 7.99 (m, 2H), 7.77 (d, 2H),




7.50 (m, 3H), 6.63 (s, 1H), 3.45 (m, 2H), 1.90 (m, 1H), 0.95 (d, 6H).


A-378
DMSO-d6
13.28 (s, 1H), 12.59 (s, 1H), 8.91 (d, 1H), 8.00 (m, 2H), 7.75 (d, 2H),




7.49 (m, 3H), 6.62 (s, 1H), 4.52 (m, 1H), 2.05 (m, 2H), 1.60 (m, 6H).


A-379
DMSO-d6
13.28 (s, 1H), 12.59 (s, 1H), 8.75 (d, 1H), 8.00 (m, 2H), 7.75 (d, 2H),




7.50 (m, 3H), 6.62 (s, 1H), 4.40 (m, 1H), 1.25 (d, 6H).


A-380
DMSO-d6
13.30 (s, 1H), 12.61 (s, 1H), 8.98 (d, 1H), 7.96 (m, 2H), 7.76 (d, 2H),




7.65 (t, 1H), 7.52 (d, 2H), 6.62 (s, 1H), 4.55 (m, 1H), 3.46 (m, 2H),




3.30 (s, 3H), 3.12 (s, 2H), 1.24 (d, 3H).


A-381
DMSO-d6
13.24 (s, 1H), 12.58 (s, 1H), 8.92 (t, 1H), 7.95 (m, 2H), 7.76 (d, 2H),




7.65 (t, 1H), 7.54 (d, 2H), 6.65 (s, 1H), 3.43 (m, 2H), 1.92 (m, 1H),




0.95 (d, 6H).


A-382
DMSO-d6
13.27 (s, 1H), 12.56 (s, 1H), 8.95 (d, 1H), 7.96 (m, 2H), 7.74 (d, 2H),




7.62 (t, 1H), 7.53 (d, 2H), 6.62 (s, 1H), 4.52 (m, 1H), 2.03 (m, 2H),




1.60 (m, 6H).


A-383
DMSO-d6
13.28 (s, 1H), 12.58 (s, 1H), 8.78 (d, 1H), 7.96 (m, 2H), 7.74 (d, 2H),




7.64 (t, 1H), 7.53 (d, 2H), 6.62 (s, 1H), 4.40 (m, 1H), 1.26 (d, 6H).


A-385
DMSO-d6
13.28 (s, 1H), 12.62 (s, 1H), 8.89 (d, 1H), 7.99 (d, 1H), 7.80 (m, 1H),




7.75 (d, 2H), 7.52 (d, 2H), 7.47 (m, 1H), 7.32 (t, 1H), 6.62 (s, 1H),




4.53 (m, 1H), 2.07 (m, 2H), 1.60 (m, 6H).


A-386
DMSO-d6
13.28 (s, 1H), 12.65 (s, 1H), 8.72 (d, 1H), 7.95 (m, 1H), 7.80 (m, 1H),




7.75 (d, 2H), 7.55 (d, 2H), 7.48 (m, 1H), 7.32 (m, 1H), 6.62 (s, 1H),




4.40 (m, 1H), 1.27 (d, 6H).


A-396
DMSO-d6
13.18 (s, 1H), 12.43 (s, 1H), 9.19 (t, 1H), 7.86 (m, 2H), 7.78 (m, 2H),




7.28 (m, 3H), 6.98 (m, 2H), 6.82 (m, 1H), 6.60 (s, 1H), 4.78 (s, 2H),




3.70 (s, 3H).


A-398
DMSO-d6
13.14 (s, 1H), 12.64 (s, 1H), 9.11 (t, 1H), 7.96 (m, 1H), 7.80 (m, 3H),




7.45 (m, 1H), 7.28 (m, 4H), 7.00 (m, 2H), 6.82 (m, 1H), 6.61 (s, 1H),




4.78 (d, 2H), 3.69 (s, 3H).


A-399
DMSO-d6
13.15 (s, 1H), 12.69 (s, 1H), 9.05 (t, 1H), 7.98 (m, 1H), 7.80 (m, 3H),




7.47 (m, 1H), 7.32 (m, 4H), 7.19 (m, 3H), 6.61 (s, 1H), 4.81 (m, 2H),




2.37 (s, 3H).


A-403
DMSO-d6
13.28 (s, 1H), 12.62 (s, 1H), 9.03 (s, 1H), 7.80 (m, 4H), 7.30 (m, 2H),




6.60 (s, 1H), 1.71 (s, 9H).


A-418
DMSO-d6
13.19 (s, 1H), 12.92 (s, 1H), 8.90 (s, 1H), 8.14 (m, 2H), 7.70 (m, 1H),




7.60 (m, 1H), 7.25 (m, 3H), 6.51 (s, 1H), 2.24 (s, 3H), 1.55 (s, 9H).


A-420
DMSO-d6
13.42 (s, 1H), 12.85 (s, 1H), 8.90 (s, 1H), 7.97 (m, 2H), 7.52 (m, 3H),




7.29 (m, 1H), 6.73 (s, 1H), 1.52 (s, 9H).


A-421
DMSO-d6
13.44 (s, 1H), 12.70 (s, 1H), 8.97 (d, 1H), 8.35 (m, 2H), 8.15 (m, 1H),




8.07 (m, 1H), 7.91 (m, 2H), 7.74 (m, 2H), 6.82 (s, 1H), 4.59 (m, 1H),




2.09 (m, 2H), 1.62 (m, 6H).


A-423
DMSO-d6
13.45 (s, 1H), 12.82 (s, 1H), 8.97 (s, 1H), 8.13 (s, 1H), 8.03 (m, 1H),




7.96 (m, 2H), 7.70 (m, 2H), 7.52 (m, 1H), 6.79 (s, 1H), 1.52 (s, 9H).


A-429
DMSO-d6
13.2 (s, 1H), 12.6 (s, 1H), 9.0 (t, 1H), 8.6 (s, 1H), 8.4 (d, 1H), 8.0 (d,




1H), 7.8 (dd, 1H), 7.7 (dd, 2H), 7.3 (dd, 2H), 6.6 (s, 1H), 3.5 (dd,




2H), 3.2 (s, 3H), 1.9 (m, 1H), 0.99 (s, 3H), 0.98 (s, 3H).


A-430
DMSO-d6
13.3 (s, 1H), 12.6 (s, 1H), 8.8 (d, 1H), 8.6 (s, 1H), 8.4 (d, 1H), 8.0 (d,




1H), 7.8 (dd, 1H), 7.7 (dd, 2H), 7.3 (dd, 2H), 6.6 (s, 1H), 4.3 (m, 1H),




3.2 (s, 3H), 1.6 (m, 2H), 1.27 (d, 3H), 0.97 (t, 3H).


A-485
DMSO-d6
13.26 (s, 1H), 12.75 (s, 1H), 10.52 (s, 1H), 9.01 (s, 1H), 8.80 (s, 1H),




8.38 (m, 1H), 7.78 (m, 2H), 7.63 (m, 1H), 7.32 (m, 2H), 6.59 (s, 1H),




2.09 (s, 3H), 1.56 (s, 9H).


A-486
DMSO-d6
13.31 (s, 1H), 12.54 (s, 1H), 9.15 (s, 1H), 8.01 (m, 1H), 7.86 (s, 2H),




7.79 (m, 2H), 7.48 (m, 1H), 7.32 (m, 2H), 7.26 (m, 1H), 6.61 (s, 1H),




1.54 (s, 9H).


A-487
DMSO-d6
13.24 (s, 1H), 12.80 (s, 1H), 8.94 (s, 1H), 8.14 (m, 1H), 7.78 (m, 2H),




7.33 (m, 2H), 7.10 (m, 2H), 6.56 (s, 1H), 3.39 (m, 4H), 1.96 (m, 4H),




1.55 (s, 9H).


A-488
DMSO-d6
13.22 (s, 1H), 12.56 (s, 1H), 8.93 (m, 1H), 8.03 (m, 1H), 7.78 (m,




2H), 7.32 (m, 2H), 7.21 (m, 1H), 7.10 (m, 1H), 6.60 (s, 1H), 4.45 (m,




1H), 3.45 (m, 2H), 3.3 (s, 3H), 1.23 (m, 3H).


A-489
DMSO-d6
13.23 (s, 1H), 12.74 (s, 1H), 8.94 (s, 1H), 8.02 (m, 1H), 7.78 (m, 2H),




7.33 (m, 2H), 7.24 (m, 1H), 7.15 (m, 1H), 6.58 (s, 1H), 1.50 (s, 9H).


A-490
DMSO-d6
13.29 (s, 1H), 12.75 (s, 1H), 8.89 (m, 1H), 8.18 (m, 2H), 7.79 (m,




2H), 7.33 (m, 2H), 7.25 (m, 2H).


A-491
DMSO-d6
13.25 (s, 1H), 12.66 (s, 1H), 9.01 (m, 1H), 8.59 (s, 1H), 8.43 (m,




1H), 8.05 (m, 1H), 7.8 (m, 2H), 7.73 (m, 1H), 7.33 (m, 2H), 6.61 (s,




1H), 4.57 (m, 1H), 3.51 (m, 2H), 3.33 (s, 3H), 3.23 (s, 3H), 1.28 (m,




3H).


A-492
DMSO-d6
13.16 (s, 1H), 12.95 (s, 1H), 9.90 (s, 1H), 8.75 (m, 1H), 7.98 (m, 2H),




7.78 (m, 2H), 7.32 (m, 2H), 6.76 (m, 2H), 6.55 (s, 1H), 4.53 (m, 1H),




3.47 (m, 2H), 3.31 (s, 3H), 1.25 (m, 3H).


A-493
DMSO-d6
13.21 (s, 1H), 12.72 (s, 1H), 8.91 (m, 2H), 8.29 (m, 1H), 7.78 (m,




2H), 7.32 (m, 2H), 6.84 (m, 1H), 6.58 (s, 1H), 4.55 (m, 1H), 3.89 (s,




3H), 3.48 (m, 2H), 1.26 (m, 3H).


A-494
DMSO-d6
13.23 (s, 1H), 12.87 (s, 1H), 8.90 (m, 2H), 8.26 (m, 1H), 7.78 (m,




2H), 7.32 (m, 2H), 6.88 (m, 1H), 6.56 (s, 1H), 3.90 (s, 3H), 1.55 (s,




9H).


A-495
DMSO-d6
13.25 (s, 1H), 12.60 (s, 1H), 9.02 (m, 1H), 8.24 (m, 1H), 7.79 (m,




2H), 7.55 (m, 1H), 7.35 (m, 3H), 6.61 (s, 1H), 4.55 (m, 1H), 3.87 (s,




3H), 3.48 (m, 2H), 1.26 (m, 3H).


A-496
DMSO-d6
13.27 (s, 1H), 12.73 (s, 1H), 9.03 (s, 1H), 8.27 (m, 1H), 7.78 (m, 2H),




7.54 (m, 1H), 7.34 (m, 3H), 6.60 (s, 1H), 3.87 (s, 3H), 1.55 (s, 9H).


A-497
DMSO-d6
13.25 (s, 1H), 12.68 (s, 1H), 9.01 (m, 1H), 8.32 (m, 2H), 7.98 (m,




2H), 7.79 (m, 2H), 7.33 (m, 2H), 6.62 (s, 1H), 4.58 (m, 1H), 3.49 (m,




2H), 3.33 (s, 3H), 3.23 (s, 1H), 1.27 (m, 3H).


A-498
DMSO-d6
13.26 (s, 1H), 12.81 (s, 1H), 9.03 (s, 1H), 8.30 (m, 2H), 8.01 (m, 2H),




7.79 (m, 2H), 7.32 (m, 2H), 6.61 (s, 1H), 3.23 (s, 3H), 1.57 (s, 9H).


A-499
DMSO-d6
13.26 (s, 1H), 12.78 (s, 1H), 9.02 (s, 1H), 8.63 (s, 1H), 8.42 (m, 1H),




8.06 (m, 1H), 7.8 (m, 3H), 7.32 (m, 2H), 6.60 (s, 1H), 3.23 (s, 3H),




1.57 (s, 9H).


A-500
DMSO-d6
13.20 (s, 1H), 12.78 (s, 1H), 8.90 (m, 1H), 8.17 (m, 2H), 7.78 (m,




2H), 7.32 (m, 2H), 7.24 (m, 2H), 6.59 (s, 1H), 4.55 (m, 1H), 3.48 (m,




2H), 3.32 (s, 3H), 1.26 (m, 3H).


A-533
DMSO-d6
13.21 (s, 1H), 12.78 (s, 1H), 8.90 (m, 1H), 8.17 (m, 2H), 7.78 (m,




2H), 7.32 (m, 2H), 7.24 (m, 2H), 6.59 (s, 1H), 4.56 (m, 1H), 3.48 (m,




2H), 3.32 (s, 3H), 1.27 (m, 3H).


A-538
DMSO-d6
13.21 (s, 1H), 12.97 (s, 1H), 8.92 (s, 1H), 8.14 (m, 2H), 7.79 (m, 2H),




7.31 (m, 4H), 6.56 (s, 1H), 3.62 (m, 2H), 1.48 (s, 6H).


A-539
DMSO-d6
13.19 (s, 1H), 13.06 (s, 1H), 8.81 (s, 1H), 8.07 (m, 2H), 7.78 (m, 2H),




7.32 (m, 2H), 6.98 (m, 2H), 6.54 (s, 1H), 3.79 (s, 3H), 1.55 (s, 9H).


A-540
DMSO-d6
13.22 (s, 1H), 12.96 (s, 1H), 8.88 (s, 1H), 7.78 (m, 2H), 7.69 (m, 2H),




7.36 (m, 3H), 7.06 (m, 1H), 6.56 (s, 1H), 3.78 (s, 3H), 1.56 (s, 9H).


A-543
DMSO-d6
12.93 (s, 1H), 10.38 (s, 1H), 8.92 (m, 1H), 7.97 (m, 1H), 7.60 (m,




1H), 7.53 (m, 1H), 7.44 (m, 1H), 7.2 (m, 2H), 7.15 (m, 1H), 6.18 (s,




1H), 4.59 (m, 2H), 3.64 (s, 3H).


A-550
DMSO-d6
13.20 (s, 1H), 13.04 (s, 1H), 8.85 (s, 1H), 8.01 (m, 2H), 7.79 (m, 2H),




7.33 (m, 2H), 7.25 (m, 2H), 6.56 (s, 1H), 2.34 (s, 3H), 1.55 (s, 9H).


A-552
DMSO-d6
13.21 (s, 1H), 13.02 (s, 1H), 8.87 (s, 1H), 7.93 (m, 2H), 7.80 (m, 2H),




7.32 (m, 4H), 6.56 (s, 1H), 2.35 (s, 3H), 1.56 (s, 9H).


A-554
DMSO-d6
13.22 (s, 1H), 12.89 (s, 1H), 8.92 (s, 1H), 7.85-7.71 (m, 4H), 7.35 (m,




3H), 6.57 (s, 1H), 2.27 (s, 3H), 1.55 (s, 9H).


A-556
DMSO-d6
13.21 (s, 1H), 12.94 (s, 1H), 8.89 (s, 1H), 8.0 (m, 2H), 7.79 (m, 2H),




7.31 (m, 2H), 7.21 (m, 1H), 6.56 (s, 1H), 2.27 (s, 3H), 1.55 (s, 9H).









Example 6
Preparation of Exemplary Substituted Pyrazole Compounds
The substituted pyrazole compounds 3-(tert-Butyl)-4-fluoro-1H-pyrazol-5-amine and 3-(3-fluoro-4-methoxyphenyl)-1H-pyrazol-5-amine were prepared according to the procedures described in Parts I and II below, respectively.
Part I: Preparation of 3-(tert-Butyl)-4-fluoro-1H-pyrazol-5-amine
The title compound was prepared according to the procedures described below.
Step A—Synthesis of 2-Fluoro-4,4-dimethyl-3-oxopentanenitrile




A solution of lithium hexamethyldisilazide (LHMDS) in tetrahydrofuran (THF) (1M, 33.9 mL, 2 equiv) was added dropwise to a chilled (−78° C.) solution of pivaloyl chloride (2.04 g, 1 equiv) and FCH2CN (1.00 g, 1 equiv) in THF (50 mL). The mixture was allowed to warm to room temperature and 1 N HCl was added dropwise until the solution was pH=2. The mixture was concentrated in vacuo to provide the title compound (2.40 g, 99% yield).
Step B—Synthesis of 3-(tert-Butyl)-4-fluoro-1H-pyrazol-5-amine




Hydrazine (1.29 g, 2.4 equiv) was added to a solution of 2-fluoro-4,4-dimethyl-3-oxopentanenitrile (2.40 g, 1 equiv) in ethanol (EtOH) (50 mL), and the reaction was heated at reflux overnight. The solvent was removed under reduced pressure, and the residue was dissolved in dichloromethane (DCM) and washed with water. The organic solution was dried over sodium sulfate and concentrated in vacuo to provide the title compound (1.43 g, 54% yield) which was used in the next reaction without purification.
Part II: Preparation of 3-(3-fluoro-4-methoxyphenyl)-1H-pyrazol-5-amine




3-(3-Fluoro-4-methoxyphenyl)-3-oxopropanenitrile (2.13 g, 11 mmol) and hydrazine (0.38 mL, 12.13 mmol) were combined in absolute ethanol and the reaction mixture was heated to reflux overnight. Then, the solvent and excess hydrazine were evaporated to provide the title compound in quantitative yield.
Example 7
Preparation of (S)-1-Ethoxypropan-2-amine
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (S)-tert-Butyl (1-hydroxypropan-2-yl)carbamate




To a chilled (0° C.) solution of (S)-2-amino-1-propanol (1.28 g) and triethylamine (2.73 mL, 1.15 equiv) in methanol (MeOH) (17 mL) was added di-tert-butyl dicarbonate (4.09 g, 1.1 equiv). The reaction was stirred at room temperature overnight and then concentrated in vacuo. The residue was diluted with DCM and washed with water. The organic phase was dried over sodium sulfate and concentrated in vacuo to provide the title compound (2.89 g, 97% yield).
Part II—Synthesis of (S)-tert-Butyl (1-ethoxypropan-2-yl)carbamate




To a mixture of t-butyl (2-hydroxy-1-methylethyl)carbamate (2.89 g) and tetrabutylammonium hydrogen sulfate (560 mg, 0.1 equiv) in toluene (33 mL) and 50% aqueous sodium hydroxide solution (6.6 mL, 5 equiv) was added ethyl bromide (1.85 mL, 1.5 equiv) at room temperature. The mixture was stirred at room temperature for 4 days. The reaction mixture was partitioned between water and ethyl ether. The organic layer was washed with water and brine, dried over sodium sulfate, and concentrated in vacuo to provide the crude product, which was purified by silica chromatography (9:1 to 75:25 hexanes:ethyl acetate (EtOAc)) to provide the title compound (2.00 g, 60% yield).
Part III—Synthesis of (S)-1-Ethoxypropan-2-amine




(S)-tert-Butyl (1-ethoxypropan-2-yl)carbamate (2.00 g) was dissolved in a 1:1 mixture of EtOH:4N HCl in dioxane, and the reaction mixture was stirred at room temperature for 3 days. The mixture was concentrated in vacuo to provide the title compound (987 mg (impure), 97% yield).
Example 8
Preparation of (Z)—N-((2,3-Dihydroxypropyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-4-fluorobenzamide (A-562)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-(((2,2-Dimethyl-1,3-dioxolan-4-yl)methyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-4-fluorobenzamide




The title compound was prepared from 4-fluorobenzoyl chloride, (2,2-dimethyl-1,3-dioxolan-4-yl)methanamine, and 3-(trifluoromethyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)—N-(((2,3-Dihydroxypropyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-4-fluorobenzamide (A-562)




To a solution of (Z)—N-(((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-4-fluorobenzamide (300 mg) in THF (7 mL) was added para-toluenesulfonic acid (p-TsOH) (133 mg, 1 equiv). The reaction mixture was shaken at room temperature overnight. The solution was then treated with triethylamine (100 μL), concentrated onto silica gel, and purified by silica chromatography (3:1 hexanes:EtOAc to EtOAc) to provide the title compound (115 mg (91% purity), 42% yield). MS (EI+) m/z: 389.93, 411.89; HPLC (Method B): 5.19 min
Example 9
Preparation of (Z)—N-(tert-Butylamino)((3-(2-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide (A-563)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-(((3-(2-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide




The title compound was prepared from 4-(trifluoromethyl)benzoyl chloride, 2-methylpropan-2-amine, and 3-(2-(benzyloxy)phenyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)—N-((tert-Butylamino)((3-(2-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide (A-563)




(Z)—N-(((3-(2-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide (190 mg) was dissolved in methanol (10 mL) and the solution was degassed with nitrogen. To the solution was added 10% palladium on carbon (38 mg, 0.1 equiv). The resulting mixture was subjected to hydrogen gas at 30 psi pressure in a Parr shaker. The reaction was checked periodically for completion. Upon reaction completion, the mixture was filtered through celite, and the filtrate was concentrated in vacuo to provide the crude product, which was purified by silica chromatography (9:1 to 55:45 hexanes:EtOAc) to provide the title compound (69 mg, 44% yield). 1HNMR (400 MHz, DMSO-d6) δ 12.79 (bm, 2H), 10.32 (bs, 1H), 9.05 (s, 1H), 8.28 (d, 2H), 7.82 (d, 2H), 7.61 (d, 1H), 7.19 (t, 1H), 6.95 (d, 1H), 6.86 (t, 1H), 6.53 (s, 1H), 4.06 (m, 1H), 3.14 (s, 1H), 1.56 (s, 9H); MS (EI+) m/z: 446.00, 467.97; HPLC (Method B): 6.15 min
Example 10
Preparation of (Z)—N-((tert-Butylamino)((3-(3-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide (A-564)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-(((3-(3-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide




The title compound was prepared from 4-(trifluoromethyl)benzoyl chloride, 2-methylpropan-2-amine, and 3-(3-(benzyloxy)phenyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)—N-((tert-Butylamino)((3-(3-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide (A-564)




(Z)—N-(((3-(3-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide (170 mg) was dissolved in methanol (10 mL) and the solution was degassed with nitrogen. To the solution was added 10% palladium on carbon (34 mg, 0.1 equiv). The mixture was then subjected to hydrogen gas at 30 psi pressure in a Parr shaker. The reaction was checked periodically for completion. Upon completion, the reaction mixture was filtered through celite, and the filtrate was concentrated in vacuo to provide the crude product, which was purified by silica chromatography (9:1 to 55:45 hexanes:EtOAc) to provide the title compound (77 mg, 54% yield). 1HNMR (400 MHz, DMSO-d6) δ 13.18 (s, 1H), 12.79 (s, 1H), 9.63 (s, 1H), 9.02 (s, 1H), 8.28 (d, 2H), 7.82 (d, 2H), 7.23 (m, 1H), 7.18 (m, 1H), 7.09 (m, 1H), 6.79 (m, 1H), 6.49 (s, 1H), 3.98 (m, 1H), 1.95 (s, 1H), 1.56 (s, 9H), 1.12 (m, 1H), 0.82 (m, 1H); MS (EI+) m/z: 445.94, 467.97; HPLC (Method B): 6.08 min.
Example 11
Preparation of (Z)—N-((tert-Butylamino)((3-(4-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-(((3-(4-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide




The title compound was prepared from 4-(trifluoromethyl)benzoyl chloride, 2-methylpropan-2-amine, and 3-(4-(benzyloxy)phenyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)—N-((tert-Butylamino)((3-(4-hydroxyphenyl)-1H-pyrazol-5-yl)amino)methylene)-4-(trifluoromethyl)benzamide




(Z)—N-(((3-(4-(Benzyloxy)phenyl)-1H-pyrazol-5-yl)amino)(tert-butylamino)methylene)-4-(trifluoromethyl)benzamide (150 mg) was dissolved in methanol (10 mL) and the solution was degassed with nitrogen. To the solution was added 10% palladium on carbon (30 mg, 0.1 equiv). The mixture was subjected to hydrogen gas at 60 psi pressure in a Parr shaker. The reaction was checked periodically for completion. Upon completion, the reaction mixture was filtered through celite, and the filtrate was concentrated in vacuo to provide the crude product, which was purified by silica chromatography (9:1 to 55:45 hexanes:EtOAc) to provide the title compound (85 mg, 68% yield).
Example 12
Preparation of (Z)-5-(3-(tert-Butyl)-2-(4-(trifluoromethyl)benzoyl)guanidino)-1H-pyrazole-3-carboxylic acid (A-565)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)-Methyl 5-(3-(tert-butyl)-2-(4-(trifluoromethyl)benzoyl)guanidino)-1H-pyrazole-3-carboxylate




The title compound was prepared from 4-(trifluoromethyl)benzoyl chloride, 2-methylpropan-2-amine, and methyl 5-amino-1H-pyrazole-3-carboxylate based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)-5-(3-(tert-Butyl)-2-(4-(trifluoromethyl)benzoyl)guanidino)-1H-pyrazole-3-carboxylic acid (A-565)




To a solution of (4-methyl 5-(3-(tert-butyl)-2-(4-(trifluoromethyl)benzoyl) guanidino)-1H-pyrazole-3-carboxylate (70 mg) in ethanol (3 mL) was added aqueous 2M NaOH (200 μL). The reaction was stirred at room temperature for 2 days. The solvent was removed in vacuo, and the sample was diluted with water. To the mixture was added 1N HCl (400 μL, aqueous). The resulting solid was collected by filtration, washed with water, and concentrated in vacuo to provide the title compound (32 mg, 68% yield). 1HNMR (400 MHz, DMSO-d6) δ 13.62 (bs, 1H), 12.65 (s, 1H), 8.80 (s, 1H), 8.25 (d, 2H), 7.81 (d, 2H), 6.58 (s, 1H), 1.54 (s, 9H); MS (EI+) m/z: 396.17 (M-1); HPLC (Method B): 5.48 min.
Example 13
Preparation of (Z)—N-(((3-(4-Fluorophenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)-3-(methylsulfinyl)benzamide (A-566)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-[[[3-(4-Fluorophenyl)-1H-pyrazol-5-yl]amino]-(isobutylamino)methylene]-3-methylsulfanyl-benzamide




The title compound was prepared from 3-(methylthio)benzoyl chloride, 2-methylpropan-1-amine, and 3-(4-fluorophenyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)—N-(((3-(4-Fluorophenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)-3-(methylsulfinyl)benzamide (A-566)




To an orange solution of (Z)—N-[[[3-(4-fluorophenyl)-1H-pyrazol-5-yl]amino]-(isobutylamino)methylene]-3-methylsulfanyl-benzamide (500 mg, 1.175 mmol) and ferric chloride trihydrate (9.5 mg, 0.035 mmol) in acetone:acetonitrile (1:1) was added periodic acid (295 mg, 1.3 mmol) in one portion. After stirring the resulting mixture at room temperature for ca. 21 hr, the reaction was quenched by adding aqueous sodium thiosulfate. The product was extracted with DCM, and the organic layer was washed with brine and dried over MgSO4. The drying reagent was removed by filtration, and the filtrate was concentrated onto silica gel and purified by silica chromatography (50-90% EtOAc in hexanes) to provide the title compound (77% yield). MS (EI+) m/z: 442.17.
Example 14
Preparation of (E)-N-(3-(2-chloro-4-fluorobenzyl)-4-oxo-7-(trifluoromethyl)-3,4-dihydropyrazolo[1,5-a][1,3,5]triazin-2(1H)-ylidene)-3,4-difluorobenzamide (A-567)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)—N-((2-chloro-4-fluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-3,4-difluorobenzamide




The title compound was prepared from 3,4-difluorobenzoyl chloride; (2-chloro-4-fluorophenyl)methanamine; and 3-(trifluoromethyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (E)-N-(3-(2-chloro-4-fluorobenzyl)-4-oxo-7-(trifluoromethyl)-3,4-dihydropyrazolo[1,5-a][1,3,5]triazin-2(1H)-ylidene)-3,4-difluorobenzamide (A-567)




To a solution of (Z)—N-((2-chloro-4-fluorobenzyl)amino)((3-(trifluoromethyl)-1H-pyrazol-5-yl)amino)methylene)-3,4-difluorobenzamide (50 mg, 0.11 mmol) in THF (1 mL) was added 1,1′-carbonyldiimidazole (CDI) (26 mg, 0.16 mmol). The reaction mixture was stirred at room temperature for 30 minutes. Purification by silica chromatography (EtOAc:hexanes) provided the title compound (14 mg, 27% yield). HPLC (Method A): 11.1 min.
Example 15
Preparation of (Z)-4-fluoro-N-(8-(4-fluorophenyl)-4,4-dimethyl-4,5-dihydro-1H-pyrazolo[1,5-a][1,3,5]triazepin-2(3H)-ylidene)benzamide (A-568)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)-4-fluoro-N-(((3-(4-fluorophenyl)-1H-pyrazol-5-yl)amino)((1-hydroxy-2-methylpropan-2-yl)amino)methylene)benzamide




The title compound was prepared based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)-4-fluoro-N-(8-(4-fluorophenyl)-4,4-dimethyl-4,5-dihydro-1H-pyrazolo[1,5-a][1,3,5]triazepin-2(3H)-ylidene)benzamide (A-568)




To a solution of (Z)-4-fluoro-N-(((3-(4-fluorophenyl)-1H-pyrazol-5-yl)amino)((1-hydroxy-2-methylpropan-2-yl)amino)methylene)benzamide (25 mg, 0.06 mmol) in anhydrous THF (0.5 mL) under a nitrogen atmosphere was added triphenylphosphine (19 mg, 0.07 mmol) followed by diisopropyl azodicarboxylate (DIAD) (14 μL, 0.07 mmol). The reaction mixture was stirred at room temperature for 30 minutes and purification by silica chromatography (EtOAc:hexanes) afforded the title compound (21 mg, 88% yield). LCMS (ESI). found (M-H)−, 394.15; 1H NMR (400 MHz, DMSO-d6) δ 12.91 (s, 1H), 9.35 (s, 1H), 8.18 (m, 2H), 7.75 (m, 2H), 7.31 (m, 4H), 7.17 (s, 1H), 3.75 (s, 2H), 1.39 (s, 6H).
Example 16
Preparation of (S,E)-4-fluoro-N-(2-(4-fluorophenyl)-8,9,9a,10-tetrahydro-4H-pyrazolo[1,5-a]pyrrolo[1,2-e][1,3,5]triazepin-5(7H)-ylidene)benzamide (A-569)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (S,E)-4-fluoro-N-(((3-(4-fluorophenyl)-1H-pyrazol-5-yl)amino)(2-(hydroxymethyl)pyrrolidin-1-yl)methylene)benzamide




The title compound was prepared based on synthetic procedures described in Example 4.
Part II—Synthesis of (S,E)-4-fluoro-N-(2-(4-fluorophenyl)-8,9,9a,10-tetrahydro-4H-pyrazolo[1,5-a]pyrrolo[1,2-e][1,3,5]triazepin-5(7H)-ylidene)benzamide (A-569)




To a solution of (S,E)-4-fluoro-N-(((3-(4-fluorophenyl)-1H-pyrazol-5-yl)amino)(2-(hydroxymethyl)pyrrolidin-1-yl)methylene)benzamide (30 mg, 0.07 mmol) in anhydrous THF (0.4 mL) under a nitrogen atmosphere was added triphenylphosphine (22 mg, 0.085 mmol) followed by DIAD (16 μL, 0.085 mmol). The reaction mixture was stirred at room temperature for 30 minutes and purification by silica chromatography (EtOAc:hexanes) provided the title compound (22 mg, 77% yield). LCMS (ESI). found (M-H), 406.06. HPLC (Method A): 7.11 min.
Example 17
Preparation of (Z)-3,4-Difluoro-N-(((3-(4-(hydroxymethyl)phenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)benzamide (A-570)
The title compound was prepared according to the procedures described below.
Part I—Synthesis of 4-(((tert-Butyldimethylsilyl)oxy)methyl)benzoic acid




To a solution of 4-hydroxymethylbenzoic acid (1 g) in DCM (66 mL) was added imidazole (985 mg, 2.2 equiv) followed by the addition of t-butyldimethylsilyl chloride (TBSCl) (1.09 g, 1.1 equiv). The reaction mixture was stirred at room temperature for 3 days and was diluted with DCM (50 mL) and 1 N HCl. The aqueous phase was extracted with DCM, and the combined organic extracts were dried over sodium sulfate and concentrated in vacuo to provide the title compound (1.44 g, 82% yield).
Part II—Synthesis of 4-(((tert-Butyldimethylsilyl)oxy)methyl)benzoyl chloride




4-[[t-Butyl(dimethyl)silyl]oxymethyl]benzoic acid (1.44 g) was suspended in thionyl chloride and the mixture was heated to 80° C. for 45 minutes. The resulting solution was concentrated in vacuo. Residual thionyl chloride and HCl were removed by subsequent concentration from toluene (twice). The crude residue was carried on to the next reaction step without purification.
Part III—Synthesis of 3-(4-(((tert-Butyldimethylsilyl)oxy)methyl)phenyl)-3-oxopropanenitrile




To a chilled (−78° C.) solution of acetonitrile (MeCN) (1.13 mL, 4 equiv) in THF (54 mL) was added butyllithium (BuLi) (1.62 mL, 10 M in hexanes, 3 equiv) dropwise, keeping the temperature under −60° C. The mixture was stirred for 15 minutes at −78° C. A solution of 4-(((tert-butyldimethylsilyl)oxy)methyl)benzoyl chloride (1.44 g) in THF (5 mL) was slowly added, and the reaction mixture was stirred at −78° C. for 45 minutes. The reaction was then quenched with aqueous ammonium chloride and diluted with EtOAc. The phases were separated and the organic phase was washed with brine, dried over sodium sulfate, and concentrated onto silica gel. Purification by silica chromatography (9:1 hexanes:EtOAc to EtOAc) provided the title compound (370 mg, 24% yield) and the de-silylated product (330 mg).
Part IV—Synthesis of 3-(4-(((tert-Butyldimethylsilyl)oxy)methyl)phenyl)-1H-pyrazol-5-amine




4-[t-Butyldimethylsilyloxy]benzoyl acetonitrile (370 mg) and hydrazine (0.044 mL, 1.1 equiv) were dissolved in ethanol (13 mL), and the reaction was heated at reflux overnight. The solution was concentrated in vacuo and the resulting residue was purified by silica chromatography (3:1 hexanes:EtOAc to EtOAc) to provide the title compound (160 mg, 41% yield).
Part V—Synthesis of (Z)—N-(((3-(4-(((tert-Butyldimethylsilyl)oxy)methyl)phenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)-3,4-difluorobenzamide




The title compound was prepared from 3,4-difluorobenzoyl chloride; 2-methylpropan-1-amine; and 3-(4-((tert-butyldimethylsilyloxy)methyl)phenyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part VI—Synthesis of (Z)-3,4-Difluoro-N-(((3-(4-(hydroxymethyl)phenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)benzamide (A-570)




To a solution of (Z)—N-(((3-(4-(((tert-butyldimethylsilyl)oxy)methyl)phenyl)-1H-pyrazol-5-yl)amino)(isobutylamino)methylene)-3,4-difluorobenzamide (60 mg) in THF (2 mL) was added tetrabutylammonium fluoride (TBAF) (1 M in THF, 0.122 mL, 1.1 equiv). The solution was stirred at room temperature for one hour, and partitioned between water and EtOAc. The organic phase was dried over sodium sulfate, concentrated onto silica gel, and purified by silica chromatography to provide the title compound (23 mg, 49% yield). 1HNMR (400 MHz, DMSO-d6) δ 13.18 (s, 1H), 12.60 (s, 1H), 8.96 (t, 1H), 8.00 (m, 2H), 7.70 (d, 2H), 7.48 (m, 1H), 7.40 (d, 2H), 6.60 (s, 1H), 5.23 (t, 1H), 4.50 (d, 2H), 3.43 (m, 2H), 1.92 (m, 1H), 1.00 (d, 6H); MS (EI+) m/z: 428.07; HPLC (Method B): 4.67 min.
Example 18
Preparation of (Z)-2-(4-(2-(4-Fluorobenzoyl)-3-(3-(trifluoromethyl)-1H-pyrazol-5-yl)guanidino)phenyl)acetic acid
The title compound was prepared according to the procedures described below.
Part I—Synthesis of (Z)-Methyl 2-(4-(2-(4-fluorobenzoyl)-3-(3-(trifluoromethyl)-1H-pyrazol-5-yl)guanidino)phenyl)acetate




The title compound was prepared from 4-fluorobenzoyl chloride, methyl 2-(4-aminophenyl)acetate, and 3-(trifluoromethyl)-1H-pyrazol-5-amine based on synthetic procedures described in Example 4.
Part II—Synthesis of (Z)-2-(4-(2-(4-Fluorobenzoyl)-3-(3-(trifluoromethyl)-1H-pyrazol-5-yl)guanidino)phenyl)acetic acid




To a solution of (4-methyl 2-(4-(2-(4-fluorobenzoyl)-3-(3-(trifluoromethyl)-1H-pyrazol-5-yl)guanidino)phenyl)acetate (400 mg) in methanol (20 mL) was added aqueous 6N NaOH (0.5 mL). The solution was held at room temperature overnight, concentrated in vacuo, and dissolved in water. The solution was acidified with 6 N HCl until precipitation appeared to be complete. The resulting precipitate was collected by filtration and dried in a vacuum oven. The crude product was purified by silica chromatography (99:1 DCM:AcOH to 84:15:1 DCM:MeOH:AcOH) to provide the title compound (5 mg, 1% yield).
Example 19
Exemplary compounds described in above Examples were tested for activity against F1F0-ATPase by measuring the ability of the compounds to inhibit ATP synthesis. In addition, the compounds were assessed for cytotoxicity in Ramos cells. Results of the biological activity tests are shown in Table 4 below. Inhibition of F1F0-ATPase activity in synthesizing ATP and cytotoxicity in Ramos cells were measured according to the procedures described in K. M. Johnson et al. Chemistry & Biology 2005, 12, 485-496. The symbol “NA” indicates that data was not available.











TABLE 4







Compound
ATP Syn
Ramos Cell



No.
IC50 (μM)
EC50 (μM)









A-1
<10
<10



A-2
<10
<10



A-3
<10
<10



A-4
<10
<10



A-5
<10
<10



A-6
<10
<10



A-7
<10
<10



A-8
<10
<10



A-9
<10
<10



A-10
<10
<10



A-11
<10
<10



A-12
<10
<10



A-13
<10
<10



A-14
<10
<10



A-15
<10
<10



A-16
<10
<10



A-17
<10
<10



A-18
<10
<10



A-19
<10
<10



A-20
<10
<10



A-21
<10
<10



A-22
<10
<10



A-23
<10
<10



A-24
<10
<10



A-25
<10
<10



A-26
<10
<10



A-27
<10
<10



A-28
<10
<10



A-29
<10
<10



A-30
<10
<10



A-31
<10
<10



A-32
<10
<10



A-33
<10
<10



A-34
<10
<10



A-35
<10
<10



A-36
<10
<10



A-37
<10
>10



A-38
<10
<10



A-39
<10
NA



A-40
<10
<10



A-41
<10
<10



A-42
<10
<10



A-43
<10
<10



A-44
>10
>10



A-45
<10
>10



A-46
<10
<10



A-47
<10
<10



A-48
<10
<10



A-49
<10
<10



A-50
<10
<10



A-51
<10
<10



A-52
<10
<10



A-53
<10
<10



A-54
<10
<10



A-55
<10
>10



A-56
<10
>10



A-57
<10
<10



A-58
<10
>10



A-59
<10
>10



A-60
<10
<10



A-61
<10
>10



A-62
<10
<10



A-63
<10
>10



A-64
<10
<10



A-65
<10
<10



A-66
<10
<10



A-67
<10
<10



A-68
<10
<10



A-69
<10
<10



A-70
<10
<10



A-71
<10
<10



A-72
<10
<10



A-73
<10
<10



A-74
<10
<10



A-75
<10
<10



A-76
<10
<10



A-77
<10
<10



A-78
<10
<10



A-79
<10
<10



A-80
<10
<10



A-81
<10
<10



A-82
<10
<10



A-83
<10
<10



A-84
<10
>10



A-85
<10
>10



A-86
<10
>10



A-87
<10
<10



A-88
<10
<10



A-89
<10
<10



A-90
<10
<10



A-91
<10
<10



A-92
<10
<10



A-93
<10
<10



A-94
<10
<10



A-95
<10
<10



A-96
<10
<10



A-97
<10
<10



A-98
<10
<10



A-99
<10
<10



A-100
<10
<10



A-101
<10
<10



A-102
<10
<10



A-103
<10
<10



A-104
<10
>10



A-105
<10
<10



A-106
<10
<10



A-107
<10
<10



A-108
<10
<10



A-109
<10
<10



A-110
<10
<10



A-111
<10
<10



A-112
<10
>10



A-113
<10
<10



A-114
<10
<10



A-115
<10
<10



A-116
<10
<10



A-117
<10
<10



A-118
<10
<10



A-119
<10
<10



A-120
<10
<10



A-121
<10
>10



A-122
<10
<10



A-123
<10
<10



A-124
<10
>10



A-125
<10
<10



A-126
>10
>10



A-127
<10
>10



A-128
<10
<10



A-129
<10
<10



A-130
<10
<10



A-131
<10
<10



A-132
<10
<10



A-133
<10
<10



A-134
<10
<10



A-135
<10
<10



A-136
<10
<10



A-137
<10
<10



A-138
<10
<10



A-139
>10
>10



A-140
>10
>10



A-141
<10
<10



A-142
<10
<10



A-143
<10
<10



A-144
<10
<10



A-145
<10
<10



A-146
<10
<10



A-147
<10
<10



A-148
>10
>10



A-149
<10
<10



A-150
<10
<10



A-151
<10
>10



A-152
<10
<10



A-153
<10
<10



A-154
<10
<10



A-155
<10
<10



A-156
>10
>10



A-157
>10
>10



A-158
>10
>10



A-159
<10
<10



A-160
<10
>10



A-161
>10
>10



A-162
<10
<10



A-163
<10
<10



A-164
<10
<10



A-165
>10
>10



A-166
<10
<10



A-167
<10
<10



A-168
<10
<10



A-169
<10
<10



A-170
<10
<10



A-171
<10
<10



A-172
<10
<10



A-173
<10
<10



A-174
<10
<10



A-175
<10
>10



A-176
<10
<10



A-177
<10
<10



A-178
<10
<10



A-179
>10
>10



A-180
<10
>10



A-181
<10
<10



A-182
<10
<10



A-183
<10
<10



A-184
>10
>10



A-185
<10
<10



A-186
>10
>10



A-187
<10
<10



A-188
<10
>10



A-189
<10
<10



A-190
>10
>10



A-191
>10
>10



A-192
<10
<10



A-193
<10
>10



A-194
NA
>10



A-195
<10
<10



A-196
<10
<10



A-197
<10
<10



A-198
<10
<10



A-199
<10
>10



A-200
<10
>10



A-201
<10
>10



A-202
<10
>10



A-203
<10
<10



A-204
<10
<10



A-205
<10
<10



A-206
<10
<10



A-207
>10
>10



A-208
>10
>10



A-209
<10
<10



A-210
<10
<10



A-211
<10
>10



A-212
<10
<10



A-213
<10
<10



A-214
<10
<10



A-215
>10
>10



A-216
<10
<10



A-217
<10
<10



A-218
<10
<10



A-219
<10
<10



A-220
<10
<10



A-221
<10
<10



A-222
<10
<10



A-223
<10
<10



A-224
<10
>10



A-225
<10
<10



A-226
<10
<10



A-227
<10
>10



A-228
<10
>10



A-229
<10
<10



A-230
<10
<10



A-231
<10
<10



A-232
<10
<10



A-233
<10
<10



A-234
<10
<10



A-235
<10
<10



A-236
<10
<10



A-237
<10
<10



A-238
<10
<10



A-239
<10
>10



A-240
<10
<10



A-241
<10
<10



A-242
<10
<10



A-243
<10
<10



A-244
<10
<10



A-245
<10
<10



A-246
<10
<10



A-247
<10
<10



A-248
<10
<10



A-249
<10
<10



A-250
<10
<10



A-251
<10
<10



A-252
<10
<10



A-253
<10
<10



A-254
<10
<10



A-255
<10
<10



A-256
<10
<10



A-257
<10
<10



A-258
<10
<10



A-259
<10
<10



A-260
<10
<10



A-261
<10
<10



A-262
<10
<10



A-263
<10
<10



A-264
<10
<10



A-265
<10
<10



A-266
<10
<10



A-267
<10
<10



A-268
<10
<10



A-269
<10
<10



A-270
<10
>10



A-271
<10
<10



A-272
<10
<10



A-273
<10
<10



A-274
<10
<10



A-275
<10
<10



A-276
<10
<10



A-277
<10
>10



A-278
<10
>10



A-279
<10
<10



A-280
<10
>10



A-281
<10
<10



A-282
<10
<10



A-283
<10
<10



A-284
<10
<10



A-285
<10
<10



A-286
<10
<10



A-287
<10
<10



A-288
<10
<10



A-289
<10
<10



A-290
<10
<10



A-291
<10
<10



A-292
<10
<10



A-293
<10
<10



A-294
<10
>10



A-295
<10
<10



A-296
<10
<10



A-297
<10
<10



A-298
<10
<10



A-299
<10
<10



A-300
<10
<10



A-301
<10
<10



A-302
<10
<10



A-303
<10
<10



A-304
<10
<10



A-305
<10
<10



A-306
<10
<10



A-307
<10
<10



A-308
<10
<10



A-309
<10
<10



A-310
<10
<10



A-311
<10
<10



A-312
<10
<10



A-313
<10
<10



A-314
<10
<10



A-315
<10
>10



A-316
<10
<10



A-317
<10
<10



A-318
>10
<10



A-319
<10
<10



A-320
<10
<10



A-321
<10
<10



A-322
<10
<10



A-323
<10
<10



A-324
<10
<10



A-325
<10
<10



A-326
<10
>10



A-327
>10
>10



A-328
<10
<10



A-329
<10
<10



A-330
>10
>10



A-331
<10
<10



A-332
<10
>10



A-333
<10
>10



A-334
<10
<10



A-335
<10
<10



A-336
<10
<10



A-337
>10
>10



A-338
<10
<10



A-339
  10
>10



A-340
<10
<10



A-341
<10
<10



A-342
<10
<10



A-343
<10
>10



A-344
<10
<10



A-345
>10
>10



A-346
<10
<10



A-347
<10
<10



A-348
<10
<10



A-349
<10
<10



A-350
<10
<10



A-351
<10
<10



A-352
<10
<10



A-354
NA
<10



A-355
NA
>10



A-356
NA
<10



A-357
NA
>10



A-358
NA
<10



A-359
<10
<10



A-360
<10
<10



A-361
<10
<10



A-362
<10
<10



A-363
<10
<10



A-364
<10
<10



A-365
<10
<10



A-366
<10
<10



A-367
<10
<10



A-368
<10
<10



A-369
<10
<10



A-370
<10
<10



A-371
>10
>10



A-372
<10
>10



A-373
<10
<10



A-374
<10
<10



A-375
<10
<10



A-376
<10
<10



A-377
<10
<10



A-378
<10
<10



A-379
<10
<10



A-380
<10
<10



A-381
<10
<10



A-382
<10
<10



A-383
<10
<10



A-384
<10
<10



A-385
<10
<10



A-386
<10
<10



A-387
<10
>10



A-388
<10
<10



A-389
<10
<10



A-390
<10
>10



A-391
>10
>10



A-392
<10
<10



A-393
<10
<10



A-394
<10
<10



A-395
<10
<10



A-396
<10
<10



A-397
<10
<10



A-398
<10
<10



A-399
<10
<10



A-400
<10
<10



A-401
<10
<10



A-402
<10
<10



A-403
<10
<10



A-404
<10
>10



A-405
<10
<10



A-406
<10
>10



A-407
<10
>10



A-408
<10
<10



A-409
<10
>10



A-410
<10
>10



A-411
<10
>10



A-412
<10
>10



A-413
<10
>10



A-414
<10
<10



A-415
<10
>10



A-416
<10
>10



A-417
<10
<10



A-418
<10
<10



A-419
<10
<10



A-420
<10
<10



A-421
<10
<10



A-422
<10
<10



A-423
<10
<10



A-424
<10
<10



A-425
<10
<10



A-426
<10
>10



A-427
<10
<10



A-428
<10
>10



A-429
<10
<10



A-430
<10
<10



A-431
<10
<10



A-432
<10
>10



A-433
<10
<10



A-434
<10
>10



A-435
<10
<10



A-436
<10
>10



A-437
<10
<10



A-438
<10
<10



A-439
<10
<10



A-440
<10
<10



A-441
<10
<10



A-442
<10
<10



A-443
<10
<10



A-444
<10
>10



A-445
<10
<10



A-446
<10
>10



A-447
<10
>10



A-448
<10
<10



A-449
<10
<10



A-450
<10
<10



A-451
<10
<10



A-452
<10
<10



A-453
<10
<10



A-454
<10
<10



A-455
<10
<10



A-456
<10
<10



A-457
<10
<10



A-458
<10
<10



A-459
<10
<10



A-460
<10
<10



A-461
<10
<10



A-462
>10
>10



A-463
<10
>10



A-464
>10
>10



A-465
>10
>10



A-466
>10
>10



A-467
<10
<10



A-468
<10
<10



A-469
<10
<10



A-470
<10
>10



A-471
<10
>10



A-472
<10
>10



A-473
<10
>10



A-474
<10
>10



A-475
<10
>10



A-476
<10
>10



A-477
<10
>10



A-478
<10
<10



A-479
<10
<10



A-480
<10
>10



A-481
<10
>10



A-482
<10
<10



A-483
<10
>10



A-484
<10
<10



A-485
>10
>10



A-486
<10
<10



A-487
<10
<10



A-488
<10
<10



A-489
<10
<10



A-490
<10
<10



A-491
<10
>10



A-492
<10
<10



A-493
<10
<10



A-494
<10
<10



A-495
<10
<10



A-496
<10
<10



A-497
<10
<10



A-498
<10
<10



A-499
<10
<10



A-500
<10
<10



A-501
<10
<10



A-502
<10
<10



A-504
<10
NA



A-505
<10
<10



A-506
<10
<10



A-507
NA
<10



A-508
NA
<10



A-509
<10
<10



A-510
<10
<10



A-511
<10
<10



A-512
<10
<10



A-513
<10
<10



A-514
<10
<10



A-515
<10
<10



A-516
<10
<10



A-517
<10
<10



A-518
<10
<10



A-519
<10
<10



A-520
<10
<10



A-521
<10
<10



A-522
<10
<10



A-523
<10
>10



A-524
<10
<10



A-525
<10
<10



A-526
<10
>10



A-527
<10
<10



A-528
<10
>10



A-529
<10
>10



A-530
>10
>10



A-531
<10
>10



A-532
<10
>10



A-533
<10
<10



A-534
<10
<10



A-535
<10
<10



A-536
<10
<10



A-537
<10
<10



A-538
<10
>10



A-539
<10
<10



A-540
<10
<10



A-541
<10
<10



A-542
<10
<10



A-543
<10
<10



A-544
<10
<10



A-545
<10
<10



A-546
<10
<10



A-547
<10
<10



A-548
<10
<10



A-549
<10
<10



A-550
<10
<10



A-551
<10
<10



A-552
<10
<10



A-553
<10
<10



A-554
<10
<10



A-555
<10
<10



A-556
<10
<10



A-557
>10
>10



A-558
<10
<10



A-559
>10
>10



A-560
<10
>10



A-561
<10
<10



A-562
>10
>10



A-563
<10
<10



A-564
<10
<10



A-565
>10
>10



A-566
<10
<10



A-567
<10
<10



A-568
<10
<10



A-569
>10
>10



A-570
>10
>10



A-571
<10
<10



A-572
<10
>10



A-573
<10
>10



A-574
<10
>10



A-575
<10
<10



A-576
<10
<10










INCORPORATION BY REFERENCE
The entire disclosure of each of the patent documents and scientific articles referred to herein is incorporated by reference for all purposes.
EQUIVALENTS
The invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The foregoing embodiments are therefore to be considered in all respects illustrative rather than limiting the invention described herein. Scope of the invention is thus indicated by the appended claims rather than by the foregoing description, and all changes that come within the meaning and range of equivalency of the claims are intended to be embraced therein.

## Claims

1. A compound represented by Formula I:



including all stereoisomers, geometric isomers, and tautomers; or a pharmaceutically acceptable salt or solvate of any of the foregoing; wherein:
A1 is phenylene;
A2 is




R1 represents independently for each occurrence halogen or haloalkyl;
R2 is hydrogen or alkyl;
R3 is aryl, aralkyl, cycloalkyl, —(C(R8)2)m-cycloalkyl, heteroaryl, heteroaralkyl, heterocycloalkyl, —(C(R8)2)m-heterocycloalkyl, alkyl, haloalkyl, hydroxyalkyl, —(C(R8)2)m-alkoxyl, —(C(R8)2)m—O—(C(R8)2)m-alkoxyl, or —(C(R8)2)m—CN, wherein said aryl, aralkyl, cycloalkyl, heteroaryl, heteroaralkyl, and heterocycloalkyl are each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, hydroxyalkyl, C1-C6alkoxy, cyano, and —C1-C6alkylene-CO2R8;
R4 is hydrogen or alkyl;
R5 is hydrogen or alkyl;
R6 is alkyl, cycloalkyl, haloalkyl, cyano, aryl, aralkyl, heteroaryl, heteroaralkyl, —CO2R8, or —C(O)N(R10)(R11), wherein said cycloalkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl are each optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, hydroxyalkyl, C1-C6alkoxy, —O-aralkyl, and cyano;
R7 is hydrogen, halogen, alkyl, or haloalkyl;
R8 represents independently for each occurrence hydrogen, alkyl, or cycloalkyl; or two occurrences of R8 attached to the same carbon atom are taken together with said carbon atom to form a saturated carbocylic ring;
R10 and R11 each represent independently for each occurrence hydrogen, alkyl, or cycloalkyl; or R10 and R11 are taken together with the nitrogen atom to which they are attached to form a 3 to 7 membered heterocyclic ring optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, alkyl, cycloalkyl, and C1-C6alkoxy;
n is 1, 2, or 3; and
m is 1, 2, 3, 4, or 5.

2. The compound of claim 1, wherein the compound is a compound of Formula I or a stereoisomer, geometric isomer, or tautomer; or a pharmaceutically acceptable salt of any of the foregoing.

3. The compound of claim 2, wherein A2 is

4. The compound of claim 3, wherein R1 is chloro, fluoro, or trifluoromethyl.

5. The compound of claim 4, wherein R2 and R4 are hydrogen.

6. The compound of claim 5, wherein R3 is aryl or aralkyl, each of which is optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of halogen, haloalkyl, alkyl, and cycloalkyl.

7. The compound of claim 5, wherein R3 is phenyl substituted with 1 or 2 substituents independently selected from the group consisting of chloro, fluoro, and trifluoromethyl.

8. The compound of claim 7, wherein R5 is hydrogen.

9. The compound of claim 8, wherein R6 is alkyl, haloalkyl, cyano, or aryl, wherein said aryl is optionally substituted with 1 or 2 substituents independently selected from the group consisting of halogen, haloalkyl, hydroxyl, and alkyl.

10. The compound of claim 5, wherein R6 is haloalkyl.

11. The compound of claim 8, wherein R6 is haloalkyl.

12. The compound of claim 8, wherein R6 is trifluoromethyl.

13. The compound of claim 11, wherein R7 is hydrogen.

14. The compound of claim 12, wherein R7 is hydrogen.

15. The compound of claim 13, wherein n is 1.

16. The compound of claim 14, wherein n is 1.

17. A pharmaceutical composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier.

18. A pharmaceutical composition comprising a compound of claim 3 and a pharmaceutically acceptable carrier.

19. A pharmaceutical composition comprising a compound of claim 7 and a pharmaceutically acceptable carrier.

20. A pharmaceutical composition comprising a compound of claim 12 and a pharmaceutically acceptable carrier.

21. A pharmaceutical composition comprising a compound of claim 16 and a pharmaceutically acceptable carrier.

22. A method of treating inflammatory bowel disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 1 to treat the inflammatory bowel disease.

23. A method of treating inflammatory bowel disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 3 to treat the inflammatory bowel disease.

24. A method of treating inflammatory bowel disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 7 to treat the inflammatory bowel disease.

25. A method of treating inflammatory bowel disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 12 to treat the inflammatory bowel disease.

26. A method of treating inflammatory bowel disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 16 to treat the inflammatory bowel disease.

27. A method of treating a disorder selected from the group consisting of psoriasis, chronic graft-versus-host disease, acute graft-versus-host disease, Crohn's disease, systemic lupus erythematosus, Sjogren's syndrome, ulcerative colitis, and epidermal hyperplasia, comprising administering to a patient in need thereof a therapeutically effective amount of a compound of claim 1 to treat the disorder.