2045 Mills Road West TEL 250-655-5800 FAX 250-655-5811

SIDNEY, BRITISH COLUMBIA, CANADA V8L 5X2 www.axysanalytical.com

AXYS Client No.: 4380

Client Address: DuPont CRG - URS Corp.

4051 Ogletown Road, Suite 300

Newark, DE, USA, 19713

The AXYS contact for these data is Kalai Pillay.

www.axysanalytical.com Page 1 of 102
PERFLUORINATED ORGANIC 

ANALYSIS 

TISSUE SAMPLES 

PROJECT NAME: WWO – Phase III 

Contract: 4380 

Data Package Identification: DPWG39837 

Analysis WG39723 

27 April 2012 

www.axysanalytical.com Page 2 of 102
DUPONT CORPORATE REMEDIATION GROUP 

TISSUE SAMPLES 

PROJECT: WWO PHASE III 

NARRATIVE 

PERFLUORINATED ORGANICS ANALYSIS 

AXYS METHOD: MLA-043 

4380: L 17643-1 

03 May 2012 

This narrative describes the analysis of one hay sample for the determination of perfluorooctanoate (PFOA) 

using high performance liquid chromatography/tandem mass spectrometry (HPLC/MS-MS). 

SAMPLE RECEIPT, STORAGE AND DESCRIPTION 

The sample was received at AXYS on the 8

th of March 2012. Details of sample conditions on receipt are 

provided on the Sample Receiving Record form included in the Sample Receiving Documentation section of 

this data package. The sample was stored at -20°C prior to sample preparation and analysis. 

SAMPLE PREPARATION AND ANALYSIS 

The sample and QC samples (a procedural blank, a lab-generated reference sample known as the Ongoing 

Precision and Recovery (OPR), a duplicate and three matrix spikes: low-level, mid-level and high-level) were 

analyzed in a single analysis batch named WG39723. The composition of the batch is shown on the 

Correlation Table and Batch List forms included in this Data Package. 

Sample preparation, instrumental analysis and analyte quantification procedures were in accordance with 

AXYS Method MLA-043: "Analytical Procedure for the Analysis of Perfluorinated Organics Compounds 

in Tissue Samples by LC/MS-MS". A method summary (MSU-043) ofAXYS Method MLA-043 is included 

in the data package. 

Approximately 19 (wet) of each sample was accurately weighed, spiked with labeled quantification standards 

and extracted in basic methanol. After extraction, the raw extract was gravimetrically split into two equal 

portions: a 1/2 portion for cleanup and a 1/2 portion for backup as per Option B. The portion of extract for 

cleanup was spiked with labeled cleanup standards and then cleaned up using SPE cartridge. The extracts 

were then spiked with labeled recovery (internal) standards, and analyzed using liquid chromatography/mass 

spectrometry (LC-MS/MS). Analyte concentrations were determined by isotope dilution/internal standard 

method, comparing the area of the quantification ion to that of the 13C-labelled standard and correcting for 

response factors. Linear regression equations with 1/X2 weighting fit were determined from a multi-point 

calibration series prepared alongside the samples. Final results are calculated in terms of the whole sample 

extracted. 

CALCULATIONS 

Target compounds were quantified using isotope dilution/internal standard method, comparing the area of the 

quantification ion to that of the 13C-labelled standard and correcting for response factors. Linear quantification 

equations were determined from a multi-point calibration series with 1/X2 weighting fit and expressed as 

below: 

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Y = slope x X + intercept 

Where: Y = response ratio = x conc. of Surr (ng/sample ) . (area of Target ) 

area of Surr 

X = concentration of target (ng/sample) 

The slope and intercept were used to convert raw peak areas in sample chromatograms to final 

concentrations as follows: 

Sample Conc. = x weight of Surr(ng/samplfj-mtercept x -- x . (

area of Target . .) ( 1 ) [ 1 J 

area of Surr slope sampleslze(g) 

where Surr is the surrogate standard 

The recovery of the surrogate standard was calculated and monitored as an indication of overall data quality. 

Final target concentrations were recovery corrected by this method of quantification. 

The standard concentrations and quantification equation can be found in the summary reports for the initial 

calibration in the Analysis Chromatography/Instrument QC section of this data package. 

The lower reporting limit (R.L.) was defined as the concentration equivalent to the lowest calibration standard 

analyzed (LMCL) or the sample specific detection limits (SOL), whichever was greater. 

LMCL = [Lowest calibration standard concentration (ng/sample) x dilution factor or extract split 

factorj/sample size (g) 

SDL = (3 x Hei~ht of noise x weight of Surr (ng/sample) _ intercept) x (_1_) x ( 1 . ) Height of Surr slope samp/eslze(L) 

Three times noise height was used to calculate SOL in the same way as calculation of analyte concentration 

using the above formula. 

The information for the final volume, quantification standard amounts, dilution factor and sample size can be 

found on the report forms in Analysis Reports/Sample Data section and/or the raw data in the Analysis 

Chromatography/Sample Data section of this data package. 

The method summary (MSU-043) ofAXYS Method MLA-043 provides QC specifications for Ongoing 

Precision and Recovery (OPR) test sample. Recovery acceptance criteria for this work are 70-130% for the 

reported compound. 

www.axysanalytical.com Page 4 of 102
For analyte concentrations greater than 10 times the reporting limits, RPD acceptance criteria for duplicates 

are 40 except for PFOA which is 20. 

A calibration point in a calibration series is excluded if the analyte recovery is not within the method 

acceptance criteria for initial calibration (Table 7 ofAXYS Method MLA-043) and/or the resulting correlation 

coefficient of the calibration curve is less than 0.95. 

REPORTING CONVENTIONS 

The AXYS contract number assigned for internal tracking was 4380. Individual samples were assigned a 

unique laboratory identifier of the form LXXXXX-X, where X is numeral. All data reports reference these 

unique AXYS IDs plus the client's sample identifier. To assist with locating data, a table correlating AXYS ID 

with the client sample number is included in this data package. 

Any extra work required and performed after the initial instrumental analysis of the sample's extract is given 

an extra "test suffix" code. The single letter code per extra work performed is added to the AXYS sample ID 

as a suffix, and is combined with any other applicable test suffix codes. The extra work codes used to report 

data in this package include: 

(A) = the parent sample for a duplicate pair 

The following laboratory qualifier flags are used in this data package: 

U = identifies a compound that was not detected 

Results are reported in concentration units of nanograms per gram (ng/g). Concentration and detection limits are 

provided to two significant figures. Analysis results for each sample are provided on Analysis Report Form 

1A12. 

QA/QC NOTES 

Samples and QC samples analyzed in each analysis batch were carried intact through the entire analytical 

process. The sample data were reviewed and evaluated in relation to the batch QC samples. 

• Sample analyte concentrations are not blank corrected. The data should be evaluated with 

consideration to the procedural blank results. 

• By virtue of the isotope dilution/internal standard quantification procedures, data are recovery 

corrected for possible losses during extraction and cleanup. 

• All linearity, CALNER, OPR, Lab Blank, Matrix Spike, duplicate and labeled compound recovery 

specifications were met with the following exception: 

The native spiking concentration of the low-level Matrix Spike (AXYS ID: WG39723-104) was very small 

relative to the concentration of the client sample WWO-X-OH-11 (HAY) (AXYS ID: L 17643-1). Consequently, 

this MS test cannot be used for quantification of spike recovery. 

The Signal/Noise (S/N) ratios were measured as '0' for some compounds in the QC samples and sample 

data. This has been determined to be a limitation of the software and would not affect the data. 

www.axysanalytical.com Page 5 of 102
ANALYTICAL DISCUSSION 

WG39723 

The initial extraction was attempted using a 2g sample size but was unsuccessful, consequently the sample 

size for the analysis batch was reduced to 19. 

A 30 minute sonication of the samples was employed during the extraction procedure. Further, it was 

observed that approximately 5g out of the 10mL of extraction solvent was recovered after the centrifuge step. 

The labeled compound recovery specifications of the method were met and overall data are deemed not to 

be significantly impacted by this variance. 

DATA PACKAGE 

This data package was assigned a unique identifier, DPWG39837, shown on the cover page of the data 

package. Included in the data package after this narrative are the following documents: 

• Method Summary 

• Sample 'Correlation Table' 

• Sample Receiving Documentation 

• Sample Homogenization Records 

• Laboratory Extraction Workup sheets for each sample 

• Sample data reports (in order ofAXYS Sample ID) 

• Laboratory QC data reports 

• Instrumental QC data reports (organized by analysis date) 

• Sample raw data (in order ofAXYS Sample ID) 

• Laboratory QC raw data 

• Instrumental QC raw data (organized by analysis date) 

I certify that this data package is in compliance with the terms and conditions of the contract, both 

technically and for completeness, except for the conditions detailed above. In addition, I certify, that 

to the best of my knowledge and belief, the data as reported are true and accurate. The following 

signature, on behalf of AXYS Analytical Services Ltd, authorizes the release of the data contained in 

this data package. 

tJ3 fY} 12 

Date tttned 

www.axysanalytical.com Page 6 of 102
AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 1 of 6 

Summary of AXYS Method MLA-043 Rev 08 Ver 02: 

Analytical Procedure for the Analysis of Perfluorinated 

Organic Compounds in Tissue Samples by LC-MS/MS 

AXYS method MLA-043 describes the analysis of perfluorinated organic compounds (PFC) in 

tissue samples. Typical detection limits are in the range of 0.5 – 1 ng/g for 2 g samples. 

Target Analytes 

Perfluorobutanoate (PFBA) Perfluorobutanesulfonate (PFBS) 

Perfluoropentanoate (PFPeA) Perfluorohexanesulfonate (PFHxS) 

Perfluorohexanoate (PFHxA) Perfluorooctanesulfonate (PFOS) 

Perfluoroheptanoate (PFHpA) Perfluorooctane sulfonamide (PFOSA) 

Perfluorooctanoate (PFOA) 

Perfluorononanoate (PFNA) 

Perfluorodecanoate (PFDA) 

Perfluoroundecanoate (PFUnA) 

Perfluorododecanoate (PFDoA) 

EXTRACTION 

The default sample size is 2 g (wet weight). Surrogate standards are added and the sample is 

extracted by shaking with methanolic potassium hydroxide solution. 

COLUMN CHROMATOGRAPHY CLEANUP 

After centrifugation a suitable aliquot of the supernatant is diluted with water and cleaned up by 

solid phase extraction (SPE) using disposable cartridges containing a weak anion exchange 

sorbent. Optional cleanup may be performed with ultra pure carbon powder. The eluate is 

spiked with recovery standards and analyzed by LC-MS/MS. Calibration solutions are 

processed through the same SPE procedure. 

INSTRUMENTAL ANALYSIS 

Analysis of sample extracts for perfluorinated organics by LC-MS/MS is performed on a high 

performance liquid chromatograph coupled to a triple quadrupole mass spectrometer. The MS is 

run at unit mass resolution in the Multiple Reaction Monitoring (MRM) mode. 

www.axysanalytical.com Page 7 of 102
AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 2 of 6 

Analytes, Ions, and Quantification References 

Target Analyte 

Typical 

Retention 

Time 

(minutes)

Parent 

Ion Mass

Daughter 

Ion Mass 

Quantified 

Against 

Perfluorobutanoate (PFBA) 5.0 213 169 13C4-PFBA

Perfluoropentanoate (PFPeA) 5.8 263 219 13C2-PFHxA

Perfluorohexanoate (PFHxA) 6.2 313 269 13C2-PFHxA

Perfluoroheptanoate (PFHpA) 6.6 363 319 13C2-PFHxA

Perfluorooctanoate (PFOA) 7.0 413 369 

(169)1

13C2-PFOA

Perfluorononanoate (PFNA) 7.4 463 419 13C5-PFNA

Perfluorodecanoate (PFDA) 7.9 513 469 13C2-PFDA

Perfluoroundecanoate (PFUnA) 8.5 563 519 13C2-PFDA

Perfluorododecanoate (PFDoA) 9.0 613 569 13C2-PFDoA 

Perfluorobutane sulfonate (PFBS) 6.3 299 80 

 (99)1

18O2-PFHxS

Perfluorohexane sulphonate (PFHxS) 7.2 399 80 

(99/119)1

18O2-PFHxS

Perfluorooctane sulfonate (PFOS) 8.2 499 80 

(99)1

13C4-PFOS 

Perfluorooctane sulfonamide (PFOSA) 9.9 498 78 13C8-PFOSA 

Surrogate Standard

13C4-Perfluorobutanoic acid (13C4-PFBA) 5.0 217 172 13C4-PFOA

13C2-Perfluorohexanoic acid (13C2-PFHxA) 6.2 315 270 13C4-PFOA

13C2-Perfluorooctanoic acid (13C2-PFOA) 7.0 415 370 13C4-PFOA 

13C5-Perfluorononanoic acid (13C5-PFNA) 7.4 468 423 13C4-PFOA 

13C2-Perfluorodecanoic acid (13C2-PFDA) 7.9 515 470 13C4-PFOA

13C2-Perfluorododecanoic acid (13C2-PFDoA) 9.0 615 570 13C4-PFOA

18O2–Perfluorohexanesulfonate (18O2-PFHxS) 7.2 403 84 

 (103)1

13C4-PFOA

13C4–Perfluorooctanesulfonate (13C4-PFOS) 8.2 503 80 

 (99)1

13C4-PFOA

13C8–Perfluorooctane sulfonamide (13C8-PFOSA) 9.9 506 78 13C4-PFOA

Clean-up Standard 

13C8-Perfluorooctanoic acid (13C8-PFOA) 7.0 421 376 13C4-PFOA

Recovery Standard 

13C2-2H-Perfluoro-2-decenoic acid 

(

13C2-PFOUEA) 7.3 459 394 - 

13C4-Perfluorooctanoic acid (13C4-PFOA) 6.9 417 372 - 

1

 Alternate transition within brackets, may be used if necessary to avoid interference. 

www.axysanalytical.com Page 8 of 102
AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 3 of 6 

CALIBRATION 

A series of at least six calibration solutions prepared in a clean reference tissue matrix similar in 

composition to the sample extract is used to establish initial multi-level calibration. The 

calibration solutions contain the analytes of interest covering the working range of the 

instrument together with labelled surrogate and recovery standards. A mid-level calibration 

solution is analyzed at least after every 20th sample to demonstrate calibration stability. All 

calibration solutions are processed through SPE cleanup. 

The concentration of the lowest level calibration standard used for quantification must exceed 

the method detection limit (MDL). 

Nominal Final Concentrations of Calibration Solutions (ng/mL) 

CAL A CAL B CAL C CAL D CAL E CAL F CAL G CAL H 

PFBA 0.125 0.312 1.25 5 25 50 125 312.5 

PFPeA 0.125 0.312 1.25 5 25 50 125 312.5 

PFHxA 0.125 0.312 1.25 5 25 50 125 312.5 

PFHpA 0.125 0.312 1.25 5 25 50 125 312.5 

PFOA 0.125 0.312 1.25 5 25 50 125 312.5 

PFNA 0.125 0.312 1.25 5 25 50 125 312.5 

PFDA 0.125 0.312 1.25 5 25 50 125 312.5 

PFUnA 0.125 0.312 1.25 5 25 50 125 312.5 

PFDoA 0.125 0.312 1.25 5 25 50 125 312.5 

PFBS 0.25 0.625 2.5 10 50 100 250 625 

PFHxS 0.25 0.625 2.5 10 50 100 250 625 

PFOS 0.25 0.625 2.5 10 50 100 250 625 

PFOSA 0.125 0.312 1.25 5 25 50 50 50 

13C4-PFBA 3 3 3 3 3 3 3 3 

13C2-PFHxA 3 3 3 3 3 3 3 3 

13C2-PFOA 9 9 9 9 9 9 9 9 

13C5-PFNA 3 3 3 3 3 3 3 3 

13C2-PFDA 3 3 3 3 3 3 3 3 

13C2-PFDoA 3 3 3 3 3 3 3 3 

18O2-PFHxS 4.5 4.5 4.5 4.5 4.5 4.5 4.5 4.5 

13C4-PFOS 4.5 4.5 4.5 4.5 4.5 4.5 4.5 4.5 

13C8-PFOSA 3 3 3 3 3 3 3 3 

13C2-PFOUEA 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 

13C4-PFOA 3 3 3 3 3 3 3 3 

1

 Concentrations shown are based on the amount added to calibration standards, with appropriate split portion (1/10, 

1/2 or entire extract) cleaned up on SPE and calculated in terms of a 4 mL extract volume. 

www.axysanalytical.com Page 9 of 102
AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 4 of 6 

ANALYTE IDENTIFICATION 

Positive identification of target PFC, surrogate standard and recovery standards require: 

• ≥ 3:1 S:N for parent ion to daughter ion transition. 

• Compound retention time falls within 0.4 minutes of the predicted retention times from the 

mean determined from the Initial Calibration. Natives with labeled surrogate standards must 

elute within 0.1 minutes of the associated labeled surrogates. 

QUANTIFICATION 

Target compounds are quantified using the internal standard method, comparing the area of the 

quantification ion to that of the 13C-labelled standard and correcting for response factors. Linear 

quantification equations are determined from a multi-point calibration series with 1/X2 weighting 

fit and expressed as below: 

Y = slope × X + intercept 

Where: Y = ⎟

⎠

⎞ ⎜

⎝

⎛ = × (ng) Surr of weight Surr of area

Target of area ratio response , and 

 X = weight of target (ng) 

The slope and intercept are used to convert raw peak areas in sample chromatograms 

to final concentrations as follows: 

Sample Conc. = ⎟

⎟

⎠

⎞

⎜

⎜

⎝

⎛

⎟

⎟

⎠

⎞

⎜

⎜

⎝

⎛ ⎟

⎠

⎞ ⎜

⎝

⎛

×

(g) size sample

1

x

slope

1 intercept- (ng) Surr of weight x

Surr of area

Target of area

 where Surr is the surrogate standard 

The recovery of the surrogate standard is calculated (by internal standard quantification 

against the recovery standard using an average RRF) and monitored as an indication of overall 

data quality. Final target concentrations are recovery corrected by this method of quantification. 

REPORTING LIMITS

Concentrations and detection limits for the target analytes are reported. Typical reporting units for 

all data are ng/g on a wet weight basis. 

The following are commonly requested reporting limits: 

Method Detection Limit (MDL) - determined as specified by EPA Fed. Reg. 40 CFR Part 136 

Appendix B (no iteration option). The 99% confidence level MDL is determined based on 

analysis of a minimum of 7 replicate matrix spikes fortified at 1-5 times the estimated detection 

limit. MDL is determined as required based on accreditation, contract and workload 

requirements. 

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AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 5 of 6 

Lower Method Calibration Limit (LMCL) - determined by prorating the concentration of the 

lowest calibration limit for sample size and extract volume. The following equation is used. 

LMCL = ((lowest level cal conc.) x (extract volume))/sample size. Typical extract volume for 

PFCs in tissues is 4 mL. 

For the analysis of PFCs it is AXYS standard to report sample concentrations using the LMCL 

as the lower reporting limit. In cases where the SDL is higher than the LMCL, the SDL will be 

used as the lower reporting limit. 

The SDL is defined as follows: Sample Specific Detection Limit or Sample Detection Limit (SDL)

– determined individually for every sample analysis run by converting the area equivalent of 3.0 

times (2.5 times for EPA 1600 series methods) the estimated chromatographic noise height to a 

concentration in the same manner that target peak responses are converted to final 

concentrations. The SDL accounts for any effect of matrix on the detection system and for 

recovery achieved through the analytical work-up. 

QUALITY ASSURANCE / QUALITY CONTROL 

All samples are analyzed in batches. The composition of a batch is detailed on a batch sheet. 

Each batch has the following composition: 

• Batch Size - Each batch consists of test samples and additional QC samples. 

• Blanks – 5% of the samples within a batch are procedural blanks. 

• Duplicates – 5% of the samples within a batch are analyzed in duplicate. 

• Matrix Spike/Matrix Spike Duplicate - may be analyzed upon client request. 

• Reference Samples - 5% of the samples within a batch are spiked reference samples. 

QC Specification Table: Procedural Blank Levels and OPR Recoveries 

Analyte 

Procedural 

Blank Level 

ng/g 1

Acceptable Matrix 

Spike 

(% Recovery) 2

Perfluorobutanoate (PFBA) < 0.5 70 – 130

Perfluoropentanoate (PFPeA) < 0.5 70 – 130

Perfluorohexanoate (PFHxA) < 0.5 70 – 130

Perfluoroheptanoate (PFHpA) < 0.5 70 – 130

Perfluorooctanoate (PFOA) < 0.5 70 – 130

Perfluorononanoate (PFNA) < 0.5 70 – 130

Perfluorodecanoate (PFDA) < 0.5 70 – 130

Perfluoroundecanoate (PFUnA) < 0.5 70 – 130

Perfluorododecanoate (PFDoA) < 0.5 70 – 130

Perfluorobutanesulfonate (PFBS) < 1 70 – 130

Perfluorohexanesulfonate (PFHxS) < 1 70 – 130

Perfluorooctanesulfonate (PFOS) < 1 70 – 130

Perfluorooctane sulfonamide (PFOSA) < 0.5 70 – 130

1

 Based on the default Option B extraction procedure a (2 gram sample sizesplit 2X). Procedural blank limits must be 

prorated accordingly for Option A or C. 2

 Marginal exceedance allowance – recovery for one compound may be 60 – 140%. 

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AXYS Analytical Services Ltd. 

MSU-043 Rev 06, 17-Oct-2011 Summary of AXYS Method MLA-043 Rev 08 Ver 02 Page 6 of 6 

QC Specification Table: Surrogate Standard Recoveries, Calibration and Samples 

Surrogate Standard Recovery Range 1

13C4-Perfluorobutyric acid (13C4-PFBA) 20 - 150% 

13C2-Perfluorocaproic acid (13C2-PFHxA) 40 - 150% 

13C2-Perfluorooctanoic acid (13C2-PFOA) 40 - 150% 

13C5-Perfluorononanoic acid (13C5-PFNA) 40 - 150% 

13C2-Perfluorodecanoic acid (13C2-PFDA) 40 - 150% 

13C2-Perfluorododecanoic acid (13C2-PFDoA) 40 - 150% 

18O2-Perfluorohexanesulfonate (18O2-PFHxS) 40 - 150% 

13C4-Perfluorooctanesulfonate (13C4-PFOS) 40 - 150% 

13C8-Perfluorooctane sulfonamide (13C8-PFOSA) 20 – 130% 

Clean-up Standard 

13C8-Perfluorooctanoic acid (13C8-PFOA) 40 - 150% 

1

 Lower surrogate recoveries may be reported for individual samples where dilution analysis or spiked sample results 

demonstrate acceptable accuracy. 

QC Specification Table: Other Parameters 

QC Parameter Specification 

Instrument Sensitivity Daily, S:N ≥3:1 for all analytes for lowest calibration standard. 

Initial Calibration 

(native compounds) 

- Daily, (1/x2

) weighed linear regression. 

- For the calculated concentrations, a maximum of 1 point per 

calibration solution with a maximum of 3 points for all the 

calibration solutions may fall within 60-140% of the actual concentration provided all remaining points, including those for 

PFOA, PFOS, and PFDA, fall within 70-130% of actual concentration. 

- Internal guideline - correlation coefficient >0.95. Calibration 

curves with lower correlation coefficient values meeting all above 

criteria may be accepted based on batch specific QC results and 

professional judgement. 

- Surrogate recoveries must fall within the same limits as for the 

samples in the table above.

Continuing Calibration 

Verification (native 

compounds) 

Every 20 samples, determined concentrations must be within 30% of 

actual concentrations. 

Surrogate recoveries must fall within the same limits as for the 

samples in the table above. 

Instrumental 

Carryover and 

Instrument 

Background 

Every Initial Calibration, Cal/Ver, or SPM: < 0.3% carryover and area 

response of analytes in instrument blank < 800 judged following two 

previous methanol blank injections. 

www.axysanalytical.com Page 12 of 102
Lab Name: AXYS Analytical Services Ltd. Project Manager: Kalai Pillay

Project Number: N/A Contract No: 4380

Project Name: WWO – Phase III AXYS Method: MLA-043

Data Package Identification: DPWG39837 Program: Tissue Samples

Client Sample No. Lab Sample ID

LAB BLANK WG39723-101

OPR WG39723-102

MATRIX SPIKE WG39723-104

MATRIX SPIKE WG39723-105

MATRIX SPIKE WG39723-106

WWO-X-OH-11 (HAY) L17643-1 WG39723-103 DUPLICATE

PERFLUORINATED ORGANIC ANALYSIS

CORRELATION TABLE

DUPONT CORPORATE REMEDIATION GROUP

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~)(~AXYS I 

2045 Mills Road West TEL: (250) 655-5800 

Axys Analytical 

Services Ltd 

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20 Recipient 30 Third Pany 5 [] Cash / Cheque 

10 Required Signature Use of this Air Waybill constitutes your agreement to the Conditions of Contract ontha back ofthis Air Waybill. Certain international treatjes, including the Warsaw Convention, may apply to this shipment and limit our liability for damage, Joss, or delay, as described in the Conditions of Contract 

Received above shipment in good order and condition. We agree to pay all charges, including Customs duties and taxes as applicable, and we agree to the Conditions of Contract as stated on the reverse side of the Recipient's Copy 

8763 6479 

[[lQ] PART 157683 5 7 0 Rev,Date9/D9 

©lS94-2009 FedEx PRINTED IN U_SA RRDA 

5391 0426 -IDNo. 

www.axysanalytical.com Page 15 of 102
Waybill : 

Date Shipped: 

~NO ~MAR-12 

AXYS Analytical Services Ltd 

SAMPLE RECEIVING RECORD 

Waybill #: 876364795391 

Date !Time Received: 08-MAR-12 11 :30 

AXYS Client & Contract # 4380;.DuPont 

Project Number: Receipt No: WB12869 

Login Number: 

Received By: KPETTINGER 

Axys Sample ID's: LJ '~,LIJ .... I I 

Log in by: 

Matrix Type: 2 Tissues 

Condition of Shipping Container: 'T~8ck Temperature upon Receipt: ·19 Celcius Sample arrived frozen on dry ice. 

Custody Seals: Shipping Containers yes~o ~) 

Samples Yes INo ) 

-~ 

Chain of Custody or Documents: 

SamplelD's 

Collection Location 

Date & Time Collection 

Collector's Name 

Sample Tags 

Sample Labels 

Sample Labels Cross Referenced toCOC 

Sample Tags Cross Referenced to Sample Labels 

Sample Tags Cross Referenced to COC 

Comments: 

Action Taken: 

Intact Yes INo 

Intact Yes INo 

Yes /'-.., 

fffVNO 

&INO 

YeslNo 

YeslNo 

Seal Numbers Yes INo 

Seal Numbers Yes INo 

Tracking Report IPacking List: 

Sample Tag Numbers 

Sample Type 

Preservative Added 

Preservation Requested 

yes. 

Y~I '~: 0 i ~s)~ 

Yes I~i) 

Yes 'e) 

Information Agrees 

Information Agrees 

Information Agrees 

Signature: .¥-:m-~' 

Thermometer ID: 3093 

c9NO 

Yes INo 

Yes INa 

www.axysanalytical.com Page 16 of 102
~](($ 

AXYS 

Axys ID versus 

AXYS Analytical Services Ltd. 

Login Chain of Custody Report (In01) 

Apr. 13, 2012 

11:23 AM 

Login Number: L 17643 

Account: 4380 DuPont 

Project: 

Client Sample Identification 

L 17643-1 

WWO-X-OH-11 (HAY) 

17-FEB-1214:20 

Tissue 

Tissue 

EDataDeliv 

D.Package 

ANY 

FC043.HALF 

HOMOGEN IZA TlON 

PFC EDD 

PFOS DATA PKG 

SAMPLE RECEIPT 

Storage: WIF-4, 6A 

Received 

08-MAR-12 

Page: 1 of 1 

Due PR 

USD 

USD 

USD 

USD 

USD 

www.axysanalytical.com Page 17 of 102
Sample Labeling Information: Batch 10: WG39726 

Method: MLAxxx 

Sample ID Sample Type and Description 

AXYS Analytical Services Ltd 

Date I &A P R-( ~ Analyst W~!YL" Contract,-,,#,,---: _--"4",,38,,,-0_ 

Original Labeling WD\6Nfl.-U 

L 17643-1 zi olorK hv.~ t" \ \ ~ ",,-I lOlA I Cirm' (bl't>wll wWo- k()t-\-II ~ [1-1AY'\ 

dr, H&"lI "'- . , 

I 

I 

FWO-305 Rev 06, 22-Feb-2010 Reviewed by ;:'l':c.- date ' 

II. -Ap , (-1'1- Page -I- of -+ 

www.axysanalytical.com Page 18 of 102
Sample Preparation - Sample weights 

WG: WG39726 

on 

sample + container sample 

container (9) (9) 

Sample ID (9) 

L 7643-1 ''IIQH Ij~;~~i J:{.W/ 

Reviewed by: )()L 

FWO-342 Rev 03. 22-Feb-2010 

Date: 

water 

discarded 

(9) 

L 

AXYS Analytical Services Ltd. 

I C-A PR-[l 

After 

sample + 

container (9) 

(9) 

~I' 81 ~.:.: .. '<::::?: 

.. ~ 

. 

/ 

Chemist: W Q/S!:?l/ 

on 

sample (gl ~ub J3.Csize 

sample (m~) 

;2on ~ :',2Si{? 

Contract: 4380 

~ 

wt (g) ~ub 

Ja(;:~~e wt (g) :O;ub ja(;:~~e wi (g) 

sample sample 

1;b·17 

, i 

Page_of] 

www.axysanalytical.com Page 19 of 102
Tissue Sample Preparation Record 

Sample 10 Sample type 

L 17643-1 OW)lOle body 1 Ofillet 

>\?~ ~ther h"y f / 

Protocol Followed OTOC II'lPFOS 

Sample 10 Sample type 

Owhole body 10filiet 

Dother 

Protocol Followed OTOC 10PFOS 

Sample 10 Sample type 

Owhole body 1 Ofillet 

Dother 

Protocol Followed OTOC 10PFOS 

Sample 10 Sample type 

Dwhole body I Dfillet 

Dother 

Protocol Followed OTOC 10PFOS 

Sample 10 Sample type 

Owhole body 1 Ofillet 

Dother 

Protocol Followed OTOC 10PFOS 

Sample 10 Sample type 

Dwhole body I Dfillet 

Dother 

Protocol Followed OTOC 10PFOS 

Reviewed by: __ S=·"'D=--G=--__ _ 

FWO-104 Rev 07, 23-Sep-2009 

AXYS Analytical Services Ltd. 

WG· WG39726 

Discard excess tissue Grinder 

/ 

Dyes ~no OG0110G03 OG0410G05 

Filleted/Djssected Virtis Blender 

Dyes EJ"no o large I 0 stator OB0610B07 

DMetals Droutine organics & ",b" 11 'If 

Discard excess tissue Grinder 

Dyes Ono OG0110G03 OG0410G05 

Filleted/Dissected Virtis Blender 

Dyes Ono Dlarge I Dstator OB0610B07 

DMetals Droutine organics 

Discard excess tissue Grinder 

Dyes Ono OG0110G03 OG0410G05 

Filleted/Dissected Virtis Blender 

Dyes Ono Dlarge I Dstator OB0610B07 

o Metals Droutine organics 

Discard excess tissue Grinder 

Dyes Ono OG0110G03 OG0410G05 

Filleted/Dissected Virtis Blender 

Dyes Ono Dlarge I Dstator OB0610B07 

DMetals D routine organics 

Discard excess tissue Grinder 

Dyes Ono OG0110G03 OG0410G05 

Filleted/Dissected Virtis Blender 

Dyes Ono Dlarge I Dstator OB0610B07 

OMetals Droutine organics 

Discard excess tissue Grinder 

Dyes Ono OG0110G03 OG0410G05 

Filleted/Dissected Virtis Blender 

Dyes Ono Dlarge I Dstator OB0610B07 

o Metals o routine organics 

Contract· 

Equipment used: 

Os),atula I ~cissors Dforceps 

10spoon Dshears Dknife 

Dstainless steel bowl 

Dother 

-

Equipment used: 

Dspatula Dscissors Dforceps 

Dspoon Dshears Dknife 

Dslainless steel bowl 

Dother 

Equipment used: 

Dspatula Dscissors o forceps 

Dspoon Dshears o knife 

Dstainiess steel bowl 

Dother 

Equipment used: 

Dspatula Dscissors Dforceps 

Dspoon Dshears o knife 

Dstainless steel bowl 

Dother 

Equipment used: 

Dspatula Dscissors Dforceps 

Dspoon Dshears Dknife 

Dstainless steel bow! 

Dother 

Equipment used: 

Dspatula Dscissors Dforceps 

Dspoon Dshears Dknife 

Dstainless steel bowl 

Dother 

, 

Dscalpel 

Dscalpel 

Dscalpel 

Dscalpel 

Dscalpel 

Dscalpel 

Page of 4 

\ 

www.axysanalytical.com Page 20 of 102
AXYS Analytical Services Ltd 

Notes: Batch ID: WG39726 Method: MLAxxx 

Date: Analyst Fraction Notes: 

I 

1/ ! ,"" Reviewed by date, __ iQn,---,-!1'f.f-~,,-i _~ ~I/~_ I 

FWO-314 Rev 04, 13-May-2008 Page L-t ofl 

www.axysanalytical.com Page 21 of 102
BATCH LIST PART ONE AXYS Analytical Services Ltd Samples, Batch Surrogate. Recovery Spiking details 

BATCH ID: WG39723 

Method 10: MLA·043 R8.02 Comments: Contract # I Sample Iwt Contract # Sample Iwt -- --

Method 10: 4380 l17643-1 ;( ''3 <I.) f"'r ~~6._ 

Method ID: MtO'QAMtol, - -- - -- -

Analyst: -

li it .... !~ -

Start Date: I ----

Batch List Author: --------------- --- __ : ~TH.~_l: i::R ----- ----- --~--- -

g~~~~~_l3y:_ 

Project Mana~er: Kalai 3 MS required: 

Low Level Spike: (Cal B level): 200uL of FC039C·AUTI §.Iass",,,,e.!},fle: N/A 

------- Medium Level Spike: (Cal 0 levell OPR level): 320uL of -

Glassware Treatment: N/A - ----- - ----- -- - ----- FC039B-AUTI 

Rotovap Type: N/A High Level Spike: (Cal G level): 20uL of FC128A·STKI 

----

Matrix: Tissue - --- --

SalllJJle 15i2:E!: 2 9 

Moistures required? NO -

Lipids required? NO -------------

Extraction Solvent: SPE I SAR is spiked with 1/2 of the SUR used in the samples. This --- - --------------- --- -

should be clearly stated on the first page of the batch list FMSProgra_m: NIA 

Save FMS discards? NIA 

Biohazard Type: i

None 

previous WG's associated with samples: 

S ikin 

Before Extraction: After Extraction: 

Product Samples required: Surrogate Cleanup Surrogate Recovery F. V. 

L17643-1. WG39723-101, 1026\\'1 (%Itz. fJbc ~3 ~F-,C",O,,4,,-3.,-He:A:=L,-F _______ 103, 104, 105, 106, '40uL FC021A.SUR/O :tt;r P '20uL FC022A·SURIO 

{fIN (D )2-

50ul FC008A-~C/olt1 4000 ul 

FC043_HALF 

FSA·004 Rev 16, 16-Feb-2010 

__ }Ql!~_£'<:.!l?!~_~~_~~Ql_~_itf_ 20uL FC022A·SUR/ 

6\" 

50ul FC008A-RECI 4000 uL 

1-________ -.-- I --

----- .. 

- - -- ----

-----------

-,-- - - - - ----- --j - -- -- --

- -

... 

- ---

._ ..... _.-

- - ----- -----

--

-- -- - - --- .. 

: 

U-vial Instructions and Comments: 

PPROP SNAP WI SAR T. spjk.~ wTth 112 of the SUR ws.d in the s.mplos_ CHECK THAT LI 

PVCD SAR ALSO USES 112 SUR 

PPROP SNAP WI 

PVCD 

Page 1 of~ 

www.axysanalytical.com Page 22 of 102
BATCH LIST PART TWO 

BATCH ID- WG39723 

QC Samples 

WG39723-101 

BLANK 

WG39723-102 

SPM 

WG39723-103 

DUP 

Type and 

Weight 

Analysis QC 

i required for: 

'FC043 HALF 

1G CANADIAN 

SPRINGS 

WATER 

OPT. A: lG 

SC520S LSOO· : 

30 (1ST-REF). 

OPT. B&C: 1G: 

SC5211 (12T- ' 

REF) 

FC043,HALF 

FC043 HALF 

USE L17643-1 ,-------

FC043 HALF 

WG39723-104 200ULOF 

MS FC039C-AUTI . 

WG39723-105 

MS 

320UL OF 

FC039B-AUT! 

FC043,HALF 

FSA-004 Rev 16, 16-Feb-2010 

Authentic spiking: Comments: 

320uLFC039B-AUTI()! -# 10 illoj ......_;;\1" 

... 

. 

...... 

• 200UL C?F FC039C-AUTJ <5\ 14 I; 

""' . 

.....•. 

Low Level Spike 

320uL FC039B-AUTJ 

.... 

- Medium Level Spike 

AXYS Analytical Services Ltd 

QC Samples 

Type and 

Weight 

WG39723-106 20UL OF 

MS FC128A-STKJ 

WG39723-107 

SAR SAR 

• 

i 

; 

, 

Analysis QC 

required for: 

FC043,HALF 

FC043_HALF 

OC samples, Authentic Spiking and Comments 

Authentic spiking: Comments: 

20UL OF FC128A-STKllH 11- 3 

81z2. HP 

High Level Spike 

....... 

: 160ul FC0398-AUTI 

. .. 

... 

Page2of~ 

www.axysanalytical.com Page 23 of 102
~)1[(1io Work Group: WG39723 4380 FC tissue 

Department: 5 PAH,LC, ... Lab AXYS 

Operator: 

Path ofWG: S 8 9 10 12 20 30 

Sample & Account Product Matrix 

L17643-1 4380 FC043.HALF T 

WG39723-101 BLANK FC043.HALF T 

Created: 13-APR-2012 Due: 

Deadline [PrJ Status Location & Comments 

24-MAY-12 [-I WG STORAGE" WJF-4, 6A. .. 

- [-I WG COMMENTS'" 1G CANADIAN SPRINGS WATER .. 

AXYS Analytical Services Ltd. 

Work Group Report (wk02) 

13-APR-2012 11 :07 AM 

Page 1 of 1 

WG39723-102 SPM FC043.HALF T - [-I WG COMMENTS = OPT. A: 1G SC5205 LSOO-30 (15T~REF). OPT. B&C: 1G SC5211 (12T-REF) ... 

WG39723-103DUP FC043.HALF T - [-I WG COMMENTS'" USE l17643-1 ,., 

WG39723-104 MS FC043.HALF T -[-] WG COMMENTS = 2QOUL OF FC039C·AUTI ... 

WG39723-10S MS FC043.HALF T - [-I WG COMMENTS'" 320UL OF FC039B-AUTf .. , 

WG39723-106 MS FC043.HALF T - [-I WG COMMENTS'" 20UL OF FC128A-STKJ .. 

WG39723-107 SAR FC043.HALF T - [-I WG COMMENTS = SAR .. 

Axys ID versus Client ID Information 

L 17643-1 WWO-X-OH-11 (HAY) {(pname WWO PHASE III) (collect 17-FEB-12 14:20:00) «» «» (rec.date 08-MAR-12)} 

QC Samples: 

WG39723-101 BLANK IG CANADIAN SPRINGS WATER 

WG39723-102 SPM OPT. A: IG SCS20S LSOO-30 (1ST-REF). OPT. B&C: IG SCS211 (12T-REF) 

WG39723-103DUP (LI7643-1) USE L17643-1 

WG39723-104 MS 200UL OF FC039C-AUT/ 

WG39723-10S MS 320UL OF FC039B-AUT/ 

WG39723-106 MS 20UL OF FCI28A-STKI 

WG39723-107 SAR SAR 

Surrogate, Recovery, and Calibration Standards, and Workgroup Comments: 

Moisture, TSS, Lipid Workgroups Associated with these Samples: 

www.axysanalytical.com Page 24 of 102
Sample Labeling Information: Batch 10: WG39723 

Method: MLA-043 R8.02 

AXYS Analytical Services Ltd 

Date 17 6ff?J:>- Analyst: '1 -& 

Sample 10 Sample Type and Description 

L17643-1 Vl-J 

d 

WG39723-103 vr'f (OUP) If 

, FWO 305 Rev 06, 22 - - Feb 2010 

f 

1'. 

v 

D J"M-€ "vf LI7hl.f?-1 

I I 

c \' 'r\ \ 

ReViewed by 2;. Ll-~r i \l date: 'h. ,. L , 

Contract>.2#",: __ ",4""38,,,0,,-

Original Labeling 

WWO X-Ol-\-II (liAr) 

WWO-'X DH-ll(i'WO 

I 

I 

~~ ,,- ;. c, " Page ~ ot _,_ n 

leI 7 

www.axysanalytical.com Page 25 of 102
Sample Labeling Information: Batch 10: WG39723 

Method· MLA-043 R8.02 

AXYS Analytical Services Ltd 

Date: I 7 AfjR I 2. Analyst '1~ 

Sample ID Sample Type and Description 

WG39723-104 (MS) Wof n. -h ~ } rvl5: eJ- t-\164-~ ~l (/ V J 

Ur-j,f A - M <; O,\- Ln6'1-~-1 WG39723-105 (MS) if iI 

WG39723-106 (MS) tAN C!AfYJ., VS MS Dr 1--11104-(-1 

t 0 ! 

FWO-305 Rev 06, 22-Feb-201 0 Reviewed by 9~ date \ 'b fll/ \1... 

Contract'" #",-: _--,N.:cO""N""E=-

Original Labeling 

wwO X-o\-\ \\(I-IA'O 

ItJWO -X-O\-\ II (HAl) 

Wwo -x-n\-\-I\ lHAn 

Page~ ofl 

www.axysanalytical.com Page 26 of 102
AXYS Analytical Services Ltd 

Sample Labeling Information: Batch ID: WG39723 

Method MLA-043 R8 02 

Sample ID Sample Type and Description 

WG39723-101 (BLANK) C le<:>..'t eotoY"\{OSS 1,'1) \A i cl. 

V 

Analyst: Ij-fa 

- (7t-o(pd cA-Y"M «,\ "'-VI k 

WG39723-102 (SPM) Ii ",llow V"\ 0 i S h"V'P -\::\s~",e - Sp'\<.ecl li"ta:h-, )( 

WG39723-107 (SAR) r. h (\ 

:'.1-'1_1<... 

FWO-305 Rev 06. 22-Feb-2010 -! \ Reviewed by date ~ I ~A~' lZ ';;:) ~ 

SN- "Ar'" 

Contracotl: #~: __ N!:!.O~N-,=-E 

Original Labeling 

c::i:lACtd1<t¥l 5ihv-tAa< wa;te.V' 

;j:\: 3'3 Lt " 

L '500-1 too '5 

'2-T- RSj:: 1\'26 

;--

--

pag~ofl 

www.axysanalytical.com Page 27 of 102
I 

Sample Wt & Moistures: Batch ID: WG39723 

Method: MLA-043 R8.02 

Sample ID 

Tare (a) 

L 17643-1 

WG39723-101 (BLANK) 

WG39723-102 (SPM) 

WG39723-103 (DUP) 

. WG39723-104 (MS) 

WG39723-105 (MS) 

WG39723-106 (MS) 

WG39723-107 (SAR) 

I 

AXYS Analytical Services Ltd 

Date:'"' AP !\12..Analyst: ~ Balance O~;:if1~ :2.022.. 

Moisture (SLA-015) 

(all weights in grams) 

Tare + Wet Tare + dry Tare (b) Tare + wet Tare + dry 

1.8 

171l;Pfll2 

Moistures into oven: Date: ____ Time: ____ Analyst: ___ Out of Oven: Date: ____ Time: ___ _ 

Extraction Information: Soxhlet: Dean Stark: Steam Distillation: Base Digest: __ 

Sample Backup 

WI or Vol (9) (SLA-OSS) 

(9 or mL) 

0.<17 N20 

{.O( 

e-fft/.o3 

O.qq 

0.'(1 

o.en 

1. 00 I 

I 

Analyst: __ _ 

Vol: mL ----' 

Drying: Drying Time: mins Analyst: Reagent: ______ _ 

Extraction Solvent used: Volume: ____ mL 

Begin: Date: Time: ____ Analyst: ____ End: Date: ____ Time: ____ Analyst: __ 

FWO-306 Rev 08. 02-Jan-2011 Reviewed by _~,---_date ---'-"-'-''l'-'--'--= 

I Page __ of ----1--

www.axysanalytical.com Page 28 of 102
AXYS Analytical Services Ltd 

Spiking Sheet: Batch 10,---: -'-W-'--G=.o:3-=:9-=-7=2""3 ____ _ Method: MLA-043 R8.02 

SLA-017 Witness name/ initials: 9,", (;(fl.N:m/5Cr 

Before extraction Before extraction Cleanup Std. Cleanup Std. Recovery Recovery 

Surr. 10: ((.Q;;2tA- 5uljQI Surr. 10: H:;.O::lIA-5i}~i Surr.: fCOU.A-~~liO I Surr.: Surr.: KOOl5ti- rz£cjC I Surr.: 

Sample 10 Date: r1 Arc \2 Date: i1= A?( I~ Date: \'{ Apr \2 Date: o t . a e. 'K ! tiP; r! ·'7 j- Date: 

Vol.: ':JQ ~L Vol.: ::10 ~L Vol.: ,"-0 ~L VoL: ~L Vol.: 50 ~L Vol.: ~L 

Syringe#: ll"iJl::J Syringe#: SII::! Syringe#: gilL Syringe#: F.V.: 4cCO uL F.v.: ~L 

Ampoule: 1 Ampoule: *' Ampoule: '3 Ampoule: Syringe#: Ii: I q Syringe#: __ 

Analyst 'J# Analyst Ampoule:~ Ampoule: __ Start time: IDS 6 Starl time: -- Start time: 1:'J4S Start time: 

Fin. lime: 1055 Fin. lime: I-::EO -- Fin. time: Fin. time: 

Time Witness Time Witness Analyst: Sf\! Analyst: Analyst: srJ Analyst: 

L 17643-1 ;\··:;(Lf Sr-< " / V. \/ 

WG39723-101 (BLANK) \ f '.::;z Lf g,U " / \I J 

WG39723-102 (SPM) \\ ',::24 c,r..,( "- / .J .J 

WG39723-103 (DUP) 1\ : ;1.4 C;u '\ V \( 

WG39723-104 (MS) , , '. de; seC / "- V .1 • 

WG39723-105 (MS) 1".-:1;:; 5(.£ / "- V . • J 

WG39723-106 (MS) \ I ;;is C;u / "- .J. -J f 

WG39723-107 (SAR) - -- \ 1· ;;L!? ~.(-< J ,) 

Authentic SId: tCO-S'H)-AVT/OI Authentic Std: F(itJ913-Avl/OI Authentic Std: rCor'lC -Aur:/O( 

Date: i+ ."?(\@ Date: l-:f. A~( j':J Date: Ii- 8~(i;) 

Sample 10 VoL: 3-;;20 ~L VoL: tbL2 ~L VoL: ;;?(':{') ~L 

Syringe#: 8:0:3 Syringe#: 81C2::i Syringe#: '6 \ 0'], 

Ampoule#: \0 Ampoule#: \0 Ampoule#: (, 

Analyst: '1tc- Analyst: VI..kI--. Analyst: ~ 

Time Witness Time Witness Time Witness 

c:.JG.1LJ -:; ;;21 - ! ()? S!'M 1\·)\ C;u- ------- IJGt-1i.( -:l;;lj - itl'1 M<; - - il>' C;Cc 

I JGt -:91. t;;13 - lOt) Me... :\.~,., \\. 0\. y--, 

[A)Gt"1"'p;;r-S - \ 0 -:J ~A\: - - ! I. '1 ::2 S:G( 

FWO-310 Rev 08, 16-Feb-2010 Reviewed by_r~J'l.t\l'i.\ __ date '\~) AVe \1, Page of 1 

www.axysanalytical.com Page 29 of 102
I 

I 

I 

I 

: 

I 

Spiking Sheet: Batch ID,--: --'-W-=--=G:..:3:..:9'-'7c.:2=-=3"----___ _ 

SLA -017 

AXYS Analytical Services Ltd 

Method: MLA-043 R8,02 

Witness name/ initials' ~(.,Q tMA ..-<" 

Before extraction Before extraction Cleanup Std. I Cleanup Std. Recovery 

Surr, ID: Surf. ID: Surr,: , Surr,: Surr.: 

Sample ID Date: Date: Date: I Date: Date: 

VoL: uL VoL: ~L VoL: ~L I Vol. ~L VoL: ~L 

Syringe#: Syringe#: Syringe#: ' Syringe#: F,V,: ~L 

Ampoule: Ampoule: Syringe#: __ Ampoule: Ampoule: Analyst: Analyst: Ampoule: __ Start time: -- Start time: -- Start time: 

i Fin, time: Fin, time: --

I : , Fin, time: 

I Time Witness I Time , Witness Analyst: I Analyst: I Analyst: 

L 17643-1 : I ! i 

WG39723-101 (BLANK) i : 

WG39723-102 (SPM) I 

WG39723-103 (DUP) I 

WG39723-104 (MS) I I 

WG39723-105 (MS) : : 

: 

WG39723-106 (MS) I I 

WG39723-107 (SAR) i I : 

I 

I ! : 

: 

: I 

I 

i I 

: : 

: 

I 

Authentic Std: FCI?l5'k STK/tli Authentic Std: I Authentic Std: 

~~le. 11: ;:;/;§c I@ ~L Date: Date: 

Sample 10 VoL: ~L : VoL: 

Syringe#: \St;;t;;) Syringe#: I Syringe#: 

Ampoule#: -S I Ampoule#: Ampoule#: 

Analyst: ~ I Analyst: Analyst: 

Time Witness ! Time ! Witness Time I 

CJ0t'l'1:;Z ;:2"1 - : 0(, 1"\, " \ \ ',-3'1 I SJA' : I 

I I 

I ! 

I 

FWO-310 Rev 08, 16-Feb-2010 \ 't. (\ \") Reviewed u,_~--,----__ date \ OMy( I L/ 

Recovery 

Surr.: 

Date: 

VoL: ~L 

F,V,: ~L 

Syringe#: __ I Ampoule: __ 

Start time: -- , Fin, time: 

Analyst: 

I 

I 

I 

I 

: 

~L 

Witness 

Page 

I 

I 

I 

I 

! 

, 

of....L... 

www.axysanalytical.com Page 30 of 102
AXYS Analytical Services Ltd 

Extract Splitting Sheet: Batch 10: WG39723 

Method' MLA 043 R8 02 

Balance #: ~\ D4 Analyst: -=S,,-,~J,--_ Date \ g Apr )1 Time ~",-,-D_ 

-

! Weight (g: 

Sample ID C-tube + Total Portion + Portion: Portion: Portion: Portion: Portion: C-tube total extract Extract c-tube '/L bi\tv r (column a) (fpl~rrr b) (~a)9~ (column c) (c-a) (direct wt) (direct wt) (direct wt) (direct wt) 

L 17643-1 I (',Ii:) \ 2S0 I 

WG39723-101 

I ",2; .,1>1 H 1'1")2-

1 I I 43.£ (BLANK) I g~u ISLi'1 I . I I i I 

WG39723-102 i 

I 

I I I 3q-, 

: 

(SPM) I ( -, () 14 '0 , a () I I ! , " ' I I , . I 

WG39723-103 I {;(,g , I SO I 5 I~ 

I 55 I I (OUP) " .IL 

I , I 

WG39723-104 (MS) I ('.1'1. 12.65 5,,'3 I 2££ I 

I 

WG39723-105 (MS) I ( 17 II ilq I 5i1 i 257 I 

t)" i I 

WG39723-106 (MS) ~ II " -16 50 L\ 2C,2. 

WG39723-107 - i I (SAR) I I i 

I I 

I I I I 

i 

I I 

i 

I i 

I I 

I 

I 

I 

I 

i 

FWO-311 Rev 06. 1-0ct-2010 Reviewed by ---"'~_ Date -'-'-'-'-'f'---""---

\ \ ---, Page _"'_ of _,_ 

www.axysanalytical.com Page 31 of 102
AXYS Analytical Services Ltd 

LC Methods Cleanup Worksheet Workgroup' WG39723 Method' MLA -043 R8 02 

SPE Pre-Elution! Conditioning: 

Date: \~ J~r\L Analyst: ~ 

SPE type: \ffiMO WA\( 

Lot#: en.; 

Reagent Lot Vol. Time 

# mL 

0.1 M Formic acid 11.'-\ S \\\ S 

0.3% NH40HI 99% 

MeOH l~S 5 \\\ ~ 

1: MeOH 

2: MeOH 

Reagent water 

type: 

Acidified water 

H20 Type ___ 

(with Hel) pH 

OCM 

2:30:68 Formic acid: 

Acetonitrile:MtBE 

2% NH40H in water 

H20 Type 

Hexane 

1: Acetonitrile 

(CH,CN) 

2: Acetonitrile 

(CH,CN) 

0.1 M HCII 5% MeOH 

MtBE 

10:20:70 NH,OH: 

MeOH: MtBE 

O.SM Sodium 

Acetate Buffer pH 4 

1: 1 Acetonitrile: 

Acetone 

Wash: / 

Load Sample: __ 

Reagent 

Reagent water 

type: 

50% 0.1 M formic 

acid/50% MeOH 

Lot 

# 

\ '\. \ 

0.1 M formic acid 1'-4 

0.3%) basic 

(NH,OH)I 

99% CS water 

20% MeOH/80% 

0.1 M formic acid 

Acidified water 

H20Type 

(with HCI>'" pfH-;--

0.1 M HCII 5% 

MeOH 

Acetonitrile (CH3CN) 

5%MeOHI 95% 

water 

H,O Type 

2% NH40H in 

water. 

H20 Type 

MtBE 

OCM 

5% acetonitrile in 

water 

H,O Type 

MeOH 

5\ 

Vol. 

mL 

c:; 

-

5 

L 

Elution: 

Time Reagent Lot 

# 

MeOH 

l3o.b 0.3% NH4OH/99% 

MeOH /M,c'6S 

\1.51 Carbon (optional) 

\ 'b2..D 

1:1 acetone:MeOH 

2 % formic acid in 

MeOH 

Acetonitrile (CH3CN) 

Hexane 

2:30:68 formic acid: 

acetonitrile: MtBE 

OCM 

10:20:70 NH,OH: 

MeOH:MtBE 

1:1 OCM:MeOH 

3:1 MtBE: MeOH 

Ethy! acetate 

9:1 MtBE: MeOH 

1 : 1 Acetonitrile: 

Acetone 

Fraction: Analyst· SN 

Reconstitute for J.lvial: 

Date: 

Analyst: 

Vol. Time Reagent Lot Vol. Time 

mL # mL 

0.1 % formic acid 

buffer 

1 : MeOH .-, 

iI 

~ 2: MeOH 

0.3% NH4OH/99% 

MeOH 

70:30 MeOH: water 

H,O Type ..•.. 

1:1 MeOH: 0.1% 

acetate buffer 

1:1 CH,CN: 

ammonium acetate, 

acetic acid buffer 

1:1 0.1% Acetic acid 

in CH3CN:Water 

H,O Type 

1: 1 0.2% Acetic acid 

in CH3CN:Water 

H20 Type 

I 

I 

I 

Reagent water I type: ___ 

MeOH= Methanol; MtBE= Methyl-t-butyl ether; OCM= Dichloromethane; MeCN= CH3CN= Acetonitrile; NH40H= Ammonium hydroxide (30% aqueous unless otherwise spec.); CS= Canadian Springs 

FWO-334 Rev 02, 19-May-2011 Reviewed by: --~".LL~------ Date: ....J'-U...Llj'-'--'-"'--__ Page ofl 

www.axysanalytical.com Page 32 of 102
LC Methods Worksheet Workgroup: WG39723 

Sample Preparation and Extraction' Analyst- '\~;: ! '-J ~ll V 

Reagent Lot# Vol. Time Vol. Time 

mL mL 

50% formic acid 1..\\ N\ d"p \DIIO 

0,05 M KOH in MeOH 31 to 12:1;0 

3% acetic acid 

0.3% (NH40H)! 99% 

MeOH 

Reagent water 

type: CS f?":lll \DD \DOO 

Carbon (50 mg ) 

acetonitrile (CH3CN) 

Formic acid cone. 

NH40H cone. 

EDTA 

0.14 M NazHP04 buffer 

pH 2 

Hel cone. 

ammonium acetate 

buffer 1 M, pH 5.0 

ammonium acetate 

buffer 1 M, pH 6.5 

ammonium acetate 

buffer 1 M, pH 4,0 

Phosphate buffer 

1 M, pH 6,0 

enzyme ~-

glucoronidase!sulfalase 

enzyme 

11- glucoronidase 

5% NH40H in water 

H20 type 

5% borate buffer 

pH 9 

0.012 g/mL FMOC-CI 

Acidified water with Hel 

(pH J 

3:1 MtBE:MeOH 

Sodium acetate buffer 

a.SM, oH 4 

10% acetate buffer pH 4 

EDC.HCI 

140mM KOH in 80:20 

MeOH:Water 

MeOH 

FWO-333 Rev 04, 26-0ct-2011 

AXYS Analytical Services Ltd 

Method: MLA-043 R8.02 

Date" V: /' f'( 0" r·,; , Vol. Time 

mL 

Sonication 

Analyst: ':If'tl 

Date: 11 Afl'tl 2 

Time: IS: '30 

#times: IX ~MI'I\. 

:So ':11< 

Shak 

Date: nAf!?l2. 

Time: Is ,,;90 

#times: l x: I/Ohi". 

Oerivatization 

Analyst: 

Date: 

Start time: 

End time: 

Millipore: 

Analyst: 

Date: 

Time: 

Filtration: 

Analyst: 

Date: 

Time: 

Fraction: 

Extract pH check/adjust 

1. pH '" £.5 

Date: \'{, Ao,I2 

Time: \oL\D 

Analyst: SN 

2.pH 

Date: 

Time: 

Analyst: 

3.pH 

Date: 

Time: 

Analyst: 

Incubation: 

Analyst: 

Date: 

Start time: 

End time: 

Reviewed by: __ '-"'-'-_______ Date: --'-"LL"i''--'----

Manifold # p':';, 

Analvst "oN Date: \is hoc no.· 

Sample 10 

L 17643-1 

WG39723-101 (BLANK) 

WG39723-102 (SPM) 

WG39723-103 (DUP) 

WG39723-104 (MS) 

WG39723-105 (MS) 

WG39723-106 (MS) 

WG39723-107 (SAR) 

Position 

I 

L 

? 

4 

5 

(; 

-, 

I 

~ 

, Page~ 

#filter 

units 

I 

I 

I 

www.axysanalytical.com Page 33 of 102
AXYS Analytical Services Ltd 

Notes: Batch 10: WG39723 Method: MLA-043 R8.02 

Date: Analyst Fraction Notes: ". r>Afe·f/. 

::. J.\?R 20lZ viP 1s"""oIp !..I -too \",\..--I and. ~ i2,. '? (.., I \ ~ tI"'- IS" '" I -tube. 'e,\l<ol-l--.c 6 B 1".\"<.. <t ,,~J. 1<1 uJ ".d. ~ 

I eircu 10.f"'l UJ n += y .... d. V< "'- -\-I-e... 3 "- ,n!'" s,'?-e. +0 \ " d.,-,~ -+~ ~ _"I*o~ '\ VH l+a-tl ""'1'1 , ~ 

I"lAf~ 12 'HlA (V \r.., I Q 6"-\,,,,", waA. '>D V\ " u..:t u -I'M :<l Co '" '''" 0--:< per- Mcvr tl1 a . P , 

I Z ADe '~L sI'J Arlee ct~.t\i{"I\;n" hr. <;aty",nl, s Wt'rt. cttcuo,t, l and Iv 4 ~ or -I: xhacl- w"~ obf";n-rc. T~csc S!!."'nirs 

\!\It.fC. DfrbSCd v An.,," j wi-t\-, 0. sDutul1j 5q UCCL,nu thc sill \'Cd 00+ of fh( heUJ ad ((()iri~J~cd 

:M~\( , i\:c\'\'n' \ or <\66\t\&I\"\ l~xb1+ . ~ 

v I\j \ c\ 

J J ~ 

J -.J 

Reviewed by SN dale 15Apr 12 

FWO-314 Rev 04. 13-May-2008 Page Of~ 

www.axysanalytical.com Page 34 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_L17643-1_Form1A_FC2G_150S25_SJ1451385.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY)

Sample Collection:

17-Feb-2012 14:20

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: L17643-1 (A)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:16:54 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 25

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g (wet weight basis)

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA 71 1.0 (L) 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_L17643-1_Form1A_FC2G_150S25_SJ1451385.html)

www.axysanalytical.com Page 35 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_L17643-1_Form2_FC2G_150S25_SJ1451385.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY)

Sample Collection:

17-Feb-2012 14:20

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: L17643-1 (A)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:16:54 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 25

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 41 57 7:06

CLEANUP STANDARD

13C8-PFOA 12 11 93 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_L17643-1_Form2_FC2G_150S25_SJ1451385.html)

www.axysanalytical.com Page 36 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-103_Form1A_FC2G_150S26_SJ1451386.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY)

(Duplicate)

Sample Collection:

17-Feb-2012 14:20

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-103 (DUP L17643-1)

Matrix: TISSUE Sample Size: 0.990 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:33:47 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 26

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g (wet weight basis)

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA 61 1.1 (S) 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-103_Form1A_FC2G_150S26_SJ1451386.html)

www.axysanalytical.com Page 37 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-103_Form2_FC2G_150S26_SJ1451386.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY)

(Duplicate)

Sample Collection:

17-Feb-2012 14:20

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-103 (DUP L17643-1)

Matrix: TISSUE Sample Size: 0.990 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:33:47 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 26

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 50 69 7:06

CLEANUP STANDARD

13C8-PFOA 12 13 100 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-103_Form2_FC2G_150S26_SJ1451386.html)

www.axysanalytical.com Page 38 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: RPD.xsl; Created: 27-Apr-2012 13:08:41; Application: XMLTransformer-1.12.13;

Report Filename: RPD_FC_LC_PFOA-RPD_WG39723-103_L17643-1_.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

PERFLUORINATED ORGANICS ANALYSIS REPORT

RELATIVE PERCENT DIFFERENCE

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380

Client ID: WWO-X-OH-11 (HAY) Concentration Units: ng/g (wet weight basis)

L17643-1 (A) WG39723-103

COMPOUND LAB

FLAG 1

CONC.

FOUND

LAB

FLAG 1

CONC.

FOUND

MEAN RELATIVE

PERCENT

DIFFERENCE

PFOA 71 61 66 16

Page 1 of 1 (WG39723 - RPD_FC_LC_PFOA-RPD_WG39723-103_L17643-1_.html)

www.axysanalytical.com Page 39 of 102
(1) Where applicable, custom lab flags have been used on this report; U = not detected at RL.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-101_Form1A_FC2G_150S23_SJ1451381.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

Lab Blank

Sample Collection:

N/A

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. N/A

Contract No.: 4380 Lab Sample I.D.: WG39723-101

Matrix: AQUEOUS (TISSUE METHOD) Sample Size: 1.00 g

Sample Receipt Date: N/A Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 02:43:22 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 23

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA U 1.0 (L)

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-101_Form1A_FC2G_150S23_SJ1451381.html)

www.axysanalytical.com Page 40 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-101_Form2_FC2G_150S23_SJ1451381.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

Lab Blank

Sample Collection:

N/A

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. N/A

Contract No.: 4380 Lab Sample I.D.: WG39723-101

Matrix: AQUEOUS (TISSUE METHOD) Sample Size: 1.00 g

Sample Receipt Date: N/A Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 02:43:22 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 23

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 80 110 7:06

CLEANUP STANDARD

13C8-PFOA 12 12 100 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-101_Form2_FC2G_150S23_SJ1451381.html)

www.axysanalytical.com Page 41 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: FC-Form8A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-102_Form8A_SJ1451375.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 8A

PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR)

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Contract No.: 4380 Lab Sample I.D.: WG39723-102

Matrix: TISSUE Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 01:36:19 Column ID: C18MS

Extract Volume (uL): 4000 OPR Data Filename: FC2G_150 S: 19

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

ALL CONCENTRATIONS REPORTED ON THIS FORM ARE CONCENTRATIONS IN EXTRACT, BASED ON A 1 mL EXTRACT VOLUME.

COMPOUND

LAB

FLAG 1

SPIKE CONC.

(ng/mL)

CONC.

FOUND

(ng/mL) % RECOVERY

RETENTION

TIME

PFOA 40 43 110 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-102_Form8A_SJ1451375.html)

www.axysanalytical.com Page 42 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: FC-Form8B.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-102_Form8B_SJ1451375.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 8B

PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR)

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Contract No.: 4380 Lab Sample I.D.: WG39723-102

Matrix: TISSUE Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 01:36:19 Column ID: C18MS

Extract Volume (uL): 4000 OPR Data Filename: FC2G_150 S: 19

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

ALL CONCENTRATIONS REPORTED ON THIS FORM ARE CONCENTRATIONS IN EXTRACT, BASED ON A 1 mL EXTRACT VOLUME.

LABELED

COMPOUND

LAB

FLAG 1

SPIKE CONC.

(ng/mL)

CONC.

FOUND

(ng/mL) % RECOVERY

RETENTION

TIME

13C2-PFOA 72 71 98 7:06

CLEANUP STANDARD

13C8-PFOA 12 12 99 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-102_Form8B_SJ1451375.html)

www.axysanalytical.com Page 43 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-104_Form1A_FC2G_150S27_SJ1451387.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-104 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:50:25 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 27

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g (wet weight basis)

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA 63 1.0 (L) 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-104_Form1A_FC2G_150S27_SJ1451387.html)

www.axysanalytical.com Page 44 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-104_Form2_FC2G_150S27_SJ1451387.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-104 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 03:50:25 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 27

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 55 76 7:06

CLEANUP STANDARD

13C8-PFOA 12 12 100 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-104_Form2_FC2G_150S27_SJ1451387.html)

www.axysanalytical.com Page 45 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R% = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: MS.xsl; Created: 27-Apr-2012 13:08:32; Application: XMLTransformer-1.12.13;

Report Filename: MS_FC_LC_PFOA-MS-MSD_WG39723-104_L17643-1_Form8C.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 8C

PERFLUORINATED ORGANICS MATRIX SPIKE (MS)

ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-104 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Extraction Date: 17-Apr-2012 Initial Calibration Date: 18-Apr-2012

Analysis Date: 19-Apr-2012 Time: 03:50:25 Instrument ID: LC MS/MS

Extract Volume (uL): 4000 Column ID: C18MS

Injection Volume (uL): 15 MS Data Filename: FC2G_150 S: 27

Dilution Factor: N/A Blank Data Filename: FC2G_150 S: 23

Cal. Ver. Data Filename: FC2G_150 S: 16

ALL CONCENTRATIONS REPORTED ON THIS FORM ARE CONCENTRATIONS ON SAMPLE SIZE BASIS

COMPOUND LAB

FLAG 1

ION

ABUND.

RATIO

SPIKE

CONC

(ng/g)

SAMPLE

LAB

FLAG 1

SAMPLE

CONC

(ng/g)

CONC.

FOUND

(ng/g)

MS

R% 2

REL

%

DIFF

PFOA N/A 2.6 71 63 N/A

Page 1 of 1 (WG39723 - MS_FC_LC_PFOA-MS-MSD_WG39723-104_L17643-1_Form8C.html)

www.axysanalytical.com Page 46 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-105_Form1A_FC2G_150S28_SJ1451388.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-105 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 04:07:11 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 28

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g (wet weight basis)

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA 110 1.0 (L) 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-105_Form1A_FC2G_150S28_SJ1451388.html)

www.axysanalytical.com Page 47 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-105_Form2_FC2G_150S28_SJ1451388.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-105 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 04:07:11 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 28

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 52 72 7:06

CLEANUP STANDARD

13C8-PFOA 12 12 100 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-105_Form2_FC2G_150S28_SJ1451388.html)

www.axysanalytical.com Page 48 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R% = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: MS.xsl; Created: 27-Apr-2012 13:08:32; Application: XMLTransformer-1.12.13;

Report Filename: MS_FC_LC_PFOA-MS-MSD_WG39723-105_L17643-1_Form8C.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 8C

PERFLUORINATED ORGANICS MATRIX SPIKE (MS)

ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-105 (MS)

Matrix: TISSUE Sample Size: 0.970 g (wet)

Extraction Date: 17-Apr-2012 Initial Calibration Date: 18-Apr-2012

Analysis Date: 19-Apr-2012 Time: 04:07:11 Instrument ID: LC MS/MS

Extract Volume (uL): 4000 Column ID: C18MS

Injection Volume (uL): 15 MS Data Filename: FC2G_150 S: 28

Dilution Factor: N/A Blank Data Filename: FC2G_150 S: 23

Cal. Ver. Data Filename: FC2G_150 S: 16

ALL CONCENTRATIONS REPORTED ON THIS FORM ARE CONCENTRATIONS ON SAMPLE SIZE BASIS

COMPOUND LAB

FLAG 1

ION

ABUND.

RATIO

SPIKE

CONC

(ng/g)

SAMPLE

LAB

FLAG 1

SAMPLE

CONC

(ng/g)

CONC.

FOUND

(ng/g)

MS

R% 2

REL

%

DIFF

PFOA N/A 41 71 110 91 N/A

Page 1 of 1 (WG39723 - MS_FC_LC_PFOA-MS-MSD_WG39723-105_L17643-1_Form8C.html)

www.axysanalytical.com Page 49 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-106_Form1A_FC2G_150S29_SJ1451389.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 1A

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-106 (MS)

Matrix: TISSUE Sample Size: 1.00 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 04:23:57 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 29

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng/g (wet weight basis)

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

COMPOUND LAB FLAG 1 CONC.

FOUND

REPORTING

LIMIT (RL)2

RETENTION

TIME

PFOA 1000 1.0 (L) 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-106_Form1A_FC2G_150S29_SJ1451389.html)

www.axysanalytical.com Page 50 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R(%) = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFC_FC_LC_PFOA_WG39723-106_Form2_FC2G_150S29_SJ1451389.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 2

PERFLUORINATED ORGANICS ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-106 (MS)

Matrix: TISSUE Sample Size: 1.00 g (wet)

Sample Receipt Date: 08-Mar-2012 Initial Calibration Date: 18-Apr-2012

Extraction Date: 17-Apr-2012 Instrument ID: LC MS/MS

Analysis Date: 19-Apr-2012 Time: 04:23:57 Column ID: C18MS

Extract Volume (uL): 4000 Sample Data Filename: FC2G_150 S: 29

Injection Volume (uL): 15 Blank Data Filename: FC2G_150 S: 23

Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_150 S: 16

Concentration Units: ng absolute

This page is part of a total report that contains information necessary for accreditation compliance.

Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested.

LABELED COMPOUND LAB

FLAG 1

SPIKE

CONC.

CONC.

FOUND

R(%) 2 RETENTION

TIME

13C2-PFOA 72 52 73 7:06

CLEANUP STANDARD

13C8-PFOA 12 12 96 7:06

Page 1 of 1 (WG39723 - PFC_FC_LC_PFOA_WG39723-106_Form2_FC2G_150S29_SJ1451389.html)

www.axysanalytical.com Page 51 of 102
(1) Where applicable, custom lab flags have been used on this report.

(2) R% = percent recovery.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested.

For Axys Internal Use Only [ XSL Template: MS.xsl; Created: 27-Apr-2012 13:08:32; Application: XMLTransformer-1.12.13;

Report Filename: MS_FC_LC_PFOA-MS-MSD_WG39723-106_L17643-1_Form8C.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8 CLIENT SAMPLE NO.

WWO-X-OH-11 (HAY) (MS)

Sample Collection:

17-Feb-2012 14:20

Form 8C

PERFLUORINATED ORGANICS MATRIX SPIKE (MS)

ANALYSIS REPORT

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Project No. WWO PHASE III

Contract No.: 4380 Lab Sample I.D.: WG39723-106 (MS)

Matrix: TISSUE Sample Size: 1.00 g (wet)

Extraction Date: 17-Apr-2012 Initial Calibration Date: 18-Apr-2012

Analysis Date: 19-Apr-2012 Time: 04:23:57 Instrument ID: LC MS/MS

Extract Volume (uL): 4000 Column ID: C18MS

Injection Volume (uL): 15 MS Data Filename: FC2G_150 S: 29

Dilution Factor: N/A Blank Data Filename: FC2G_150 S: 23

Cal. Ver. Data Filename: FC2G_150 S: 16

ALL CONCENTRATIONS REPORTED ON THIS FORM ARE CONCENTRATIONS ON SAMPLE SIZE BASIS

COMPOUND LAB

FLAG 1

ION

ABUND.

RATIO

SPIKE

CONC

(ng/g)

SAMPLE

LAB

FLAG 1

SAMPLE

CONC

(ng/g)

CONC.

FOUND

(ng/g)

MS

R% 2

REL

%

DIFF

PFOA N/A 1000 71 1000 97 N/A

Page 1 of 1 (WG39723 - MS_FC_LC_PFOA-MS-MSD_WG39723-106_L17643-1_Form8C.html)

www.axysanalytical.com Page 52 of 102
________ 4:_-2:,_t5_0 __ f(-_8_A_- . 6/r.k(. 'i ts~\'<....e.-S . 

Sample List Report MassLynx 4.1 SCN627 

Sample List: G:\Masslynx\lnst_ G'LMaster.PRO\SampleDB\FC2G_150.SPL Page 1 of 1 

Last Modified: Wednesday, April 18, 2012 15:12:44 Pacific Daylight Time 

Printed: Wednesday, April 18, 2012 15:15:13 Pacific Daylight Time Page Position (1, 1) 

File Name Sample 10 File Text Bottle Signln SignOut Inj VoL 

1 FC2G 150S001 MeOH" 1,,15uL 1:8,A wt 'w 15.0 

2 FC2G -150S002 MeOH" 1,,15uL 1:8,A -- 15.0 

3 FC2G-150S003 FC-L1N-39725T-A" 1/2 1,,15uL 2:1,A 15.0 

4 FC2G-150S004 FC-L1 N-39725T -A" 1 12 1,,15uL 2:1,A 

5 FC2G -150S005 FC-L1N-39725T-B" 1/2 1" 15uL 2:1,8 . 15( 15.0 _ 

6 FC2G-150S006 FC-L1N-39725T-C" 1 12 1,,15uL 2:1,C 15.0 

7 FC2G:::: 150S007 FC-L1N-39725T-D" 1/2 1,,15uL 2:1,0 15.0 

8 F C2G _150S008 FC-L1N-39725T-E" 1/2 1,,15uL 2:1,E 15.0 ! ~ .J'C1J 

9 FC2G_150S009 FC-L1N-39725T-F" 1/2 1,,15uL 2:1,F 15.0 

10 FC2G 150S010 FC-L1N-39725T-G" 1/2 1,,15uL 2:2,A 15.0 

11 FC2G-150S011 FC-LlN-39725T-H" 1/2 1,,15uL 2:2,8 15.a. 

12 FC2G-150S012 MeOH" 1,,15uL 1:8,A 15.0 

13 FC2G::::150S013 MeOH" 1,,15uL 1:8,A 15.0 

14 FC2G 150S014 FC-39725T-SAR2" 1,,15uL 2:2,C 15.0 

15 FC2G-150S015 F C-39725T -SAR" 1" 15uL 2:2,0 

T 

15.0 

16 FC2G-150S016 FC-L1N-39725T-0" 1/2 1" 15uL 2:1,0 15.0 

17 FC2G-150S017 FC-L1N-39725T-& 1/2 1,,15uL 2:1,0 -- 15.0 

18 FC2G-150S018 WG39723-1-Ga~~ R ~~G39723, 15/4000uL 2:2,E T 15.0 

19 FC2G-150S019 WG39723-102"SPM ,G39723, 15/4000uL 2:2,F 15.0 

20 FC2G-150S020 WG39723-102,i,SPM 1 ,WG39723, 15/4000uL 2:2,F 15.0 

21 FC2G::::150S021 MeOH" 1,,15uL 1:8,A 15.0 

22 FC2G 150S022 MeOH" 1" 15uL 1:8,A 15.0 U) 

23 FC2G-150S023 WG39723-1 01 "BLK 1 ,WG39723, 15/4000uL 2:3,A 15.0 '\(:}-~\ .- \ '<;<!..- i) 

24 FC2G-150S024 WG39723-1 01 ,i,BLK 1 ,WG39723, 15/4000uL 2:3,A 15.0 

25 FC2G-150S025 L 17643-1" 1 , WG39723, 15/4000uL 2:3,8 15.0 

26 F C2G -150S026 WG39723-103"OUP 1 ,WG39723, 15/4000uL 2:3,C 15.0 

27 FC2G -1508027 WG39723-104"M8 1 ,WG39723, 15/4000uL 2:3,0 15.0 

28 FC2G -1508028 WG39723-105"MS 1 ,WG39723, 15/4000uL 2:3,E 15.0 

29 FC2G-1508029 WG39723-106"M8 1 ,WG39723, 15/4000uL 2:3,F 15.0 

30 FC2G-1508030 MeOH" 1;,15uL 1:8,A 15.0 

31 FC2G-1508031 FC-L1N-39725T-D" 1/2 1" 15uL 2:1,0 15.0" 

32 FC2G-1508032 FC-L1N-39725T-D" 1/2 1" 15uL 2:1,0 15.0 

33 FC2G -150S033 MeOH" 1,,15uL 1:8,A 15.0 

34 FC2G:::: 1508034 MeOH" 1,,15uL 1:8,A 15.0 

www.axysanalytical.com Page 53 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form3A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_18-Apr-2012_FC2G__Form3A_GS45435.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 3A

PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

CS0 Data Filename: FC2G_150 S: 4

Initial Calibration Date: 18-Apr-2012 CS1 Data Filename: FC2G_150 S: 5

Instrument ID: LC MS/MS CS2 Data Filename: FC2G_150 S: 6

LC Column ID: C18MS CS3 Data Filename: FC2G_150 S: 7

CS4 Data Filename: FC2G_150 S: 8

CS5 Data Filename: FC2G_150 S: 9

CS6 Data Filename: FC2G_150 S: 10

CS7 Data Filename: FC2G_150 S: 11

CS8 Data Filename: N/A

PERCENT RECOVERY (%)

CS0 CS1 CS2 CS3 CS4 CS5 CS6 CS7 CS8

COMPOUND LAB

FLAG 1

PFOA 92.1 119 100 108 97.4 104 94.3 84.0

Page 1 of 1 (WG39723 - PFOA_FC_LC_18-Apr-2012_FC2G__Form3A_GS45435.html)

www.axysanalytical.com Page 54 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form3B.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_18-Apr-2012_FC2G__Form3B_GS45435.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 3B

PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

CS0 Data Filename: FC2G_150 S: 4

Initial Calibration Date: 18-Apr-2012 CS1 Data Filename: FC2G_150 S: 5

Instrument ID: LC MS/MS CS2 Data Filename: FC2G_150 S: 6

LC Column ID: C18MS CS3 Data Filename: FC2G_150 S: 7

CS4 Data Filename: FC2G_150 S: 8

CS5 Data Filename: FC2G_150 S: 9

CS6 Data Filename: FC2G_150 S: 10

CS7 Data Filename: FC2G_150 S: 11

CS8 Data Filename: N/A

PERCENT RECOVERIES (%)

CS0 CS1 CS2 CS3 CS4 CS5 CS6 CS7 CS8

LABELED COMPOUND LAB

FLAG 1

13C2-PFOA 96.3 98.1 98.7 96.5 101 103 98.8 108

CLEAN-UP STANDARD

13C8-PFOA 102 97.3 102 96.7 100 99.4 105 97.4

Page 1 of 1 (WG39723 - PFOA_FC_LC_18-Apr-2012_FC2G__Form3B_GS45435.html)

www.axysanalytical.com Page 55 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form3C.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_18-Apr-2012_FC2G__Form3C_GS45435.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 3C

PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

CS0 Data Filename: FC2G_150 S: 4

Initial Calibration Date: 18-Apr-2012 CS1 Data Filename: FC2G_150 S: 5

Instrument ID: LC MS/MS CS2 Data Filename: FC2G_150 S: 6

LC Column ID: C18MS CS3 Data Filename: FC2G_150 S: 7

CS4 Data Filename: FC2G_150 S: 8

CS5 Data Filename: FC2G_150 S: 9

CS6 Data Filename: FC2G_150 S: 10

CS7 Data Filename: FC2G_150 S: 11

CS8 Data Filename: N/A

RETENTION TIMES

CS0 CS1 CS2 CS3 CS4 CS5 CS6 CS7 CS8 MEAN

RT

COMPOUND LAB

FLAG 1

PFOA 7:09 7:09 7:06 7:09 7:06 7:06 7:06 7:06 7:07

Page 1 of 1 (WG39723 - PFOA_FC_LC_18-Apr-2012_FC2G__Form3C_GS45435.html)

www.axysanalytical.com Page 56 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form3D.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_18-Apr-2012_FC2G__Form3D_GS45435.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 3D

PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

CS0 Data Filename: FC2G_150 S: 4

Initial Calibration Date: 18-Apr-2012 CS1 Data Filename: FC2G_150 S: 5

Instrument ID: LC MS/MS CS2 Data Filename: FC2G_150 S: 6

LC Column ID: C18MS CS3 Data Filename: FC2G_150 S: 7

CS4 Data Filename: FC2G_150 S: 8

CS5 Data Filename: FC2G_150 S: 9

CS6 Data Filename: FC2G_150 S: 10

CS7 Data Filename: FC2G_150 S: 11

CS8 Data Filename: N/A

RETENTION TIMES

CS0 CS1 CS2 CS3 CS4 CS5 CS6 CS7 CS8 MEAN

RT

LABELED COMPOUND LAB

FLAG1

13C2-PFOA 7:09 7:09 7:06 7:06 7:06 7:06 7:06 7:06 7:07

CLEAN-UP STANDARD

13C8-PFOA 7:06 7:09 7:06 7:06 7:06 7:06 7:06 7:06 7:06

Page 1 of 1 (WG39723 - PFOA_FC_LC_18-Apr-2012_FC2G__Form3D_GS45435.html)

www.axysanalytical.com Page 57 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_FC2G_150S16__Form4A_SJ1451309.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 4A

PERFLUORINATED ORGANICS CALIBRATION VERIFICATION

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Initial Calibration Date: 18-Apr-2012 VER Data Filename: FC2G_150 S: 16

Instrument ID: LC MS/MS Analysis Date: 19-Apr-2012

LC Column ID: C18MS Analysis Time: 00:46:01

COMPOUND LAB

FLAG 1

RETENTION TIME EXPECTED CONC.

(ng)

CONC. FOUND

(ng)

RECOVERY (%)

PFOA 7:06 40.0 42.8 107

Page 1 of 1 (WG39723 - PFOA_FC_LC_FC2G_150S16__Form4A_SJ1451309.html)

www.axysanalytical.com Page 58 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_FC2G_150S16__Form4B_SJ1451309.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 4B

PERFLUORINATED ORGANICS CALIBRATION VERIFICATION

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Initial Calibration Date: 18-Apr-2012 VER Data Filename: FC2G_150 S: 16

Instrument ID: LC MS/MS Analysis Date: 19-Apr-2012

LC Column ID: C18MS Analysis Time: 00:46:01

LABELED COMPOUND LAB

FLAG 1

RETENTION TIME EXPECTED CONC.

(ng)

CONC. FOUND

(ng)

RECOVERY (%)

13C2-PFOA 7:06 72.0 66.5 92.4

CLEANUP STANDARD

13C8-PFOA 7:06 12.0 11.9 98.8

Page 1 of 1 (WG39723 - PFOA_FC_LC_FC2G_150S16__Form4B_SJ1451309.html)

www.axysanalytical.com Page 59 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_FC2G_150S31__Form4A_SJ1451328.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 4A

PERFLUORINATED ORGANICS CALIBRATION VERIFICATION

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Initial Calibration Date: 18-Apr-2012 VER Data Filename: FC2G_150 S: 31

Instrument ID: LC MS/MS Analysis Date: 19-Apr-2012

LC Column ID: C18MS Analysis Time: 04:57:29

COMPOUND LAB

FLAG 1

RETENTION TIME EXPECTED CONC.

(ng)

CONC. FOUND

(ng)

RECOVERY (%)

PFOA 7:06 40.0 42.8 107

Page 1 of 1 (WG39723 - PFOA_FC_LC_FC2G_150S31__Form4A_SJ1451328.html)

www.axysanalytical.com Page 60 of 102
(1) Where applicable, custom lab flags have been used on this report.

These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes.

Signed: ___________Jenny Pape___________

For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 27-Apr-2012 13:08:22; Application: XMLTransformer-1.12.13;

Report Filename: PFOA_FC_LC_FC2G_150S31__Form4B_SJ1451328.html; Workgroup: WG39723; Design ID: 1817 ]

AXYS METHOD MLA-043 Rev 8

Form 4B

PERFLUORINATED ORGANICS CALIBRATION VERIFICATION

AXYS ANALYTICAL SERVICES

2045 MILLS RD., SIDNEY, B.C., CANADA

V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811

Initial Calibration Date: 18-Apr-2012 VER Data Filename: FC2G_150 S: 31

Instrument ID: LC MS/MS Analysis Date: 19-Apr-2012

LC Column ID: C18MS Analysis Time: 04:57:29

LABELED COMPOUND LAB

FLAG 1

RETENTION TIME EXPECTED CONC.

(ng)

CONC. FOUND

(ng)

RECOVERY (%)

13C2-PFOA 7:06 72.0 71.2 98.9

CLEANUP STANDARD

13C8-PFOA 7:06 12.0 12.7 106

Page 1 of 1 (WG39723 - PFOA_FC_LC_FC2G_150S31__Form4B_SJ1451328.html)

www.axysanalytical.com Page 61 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G _150-b.qld 

Last Altered: Friday, April 27, 2012 11 :33:53 Pacific Daylight Time 

Printed: Friday, April 27, 201213:05:43 Pacific Daylight Time -----------~~------------------------------------------------

ID: L 17643·1" 

Name: FC2G_150S025 

Description: 1 ,WG39723, 15/4000uL 

Date: 19-Apr·2012 

Time: 03:16:54 

PFOA·1 

FC2G _1508025 

I 

7.11 

7.13e4 

.' 276730 

min 

7.00 8.00 

1# NSI\l& 1:71:: ..•.• 

5 PFOA-1 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

PFOA·2 

FC2G _1508025 

10 

7.00 

7.11 

1.45e4 

55133 

" 

8.00 

·'.:'.I.~.,\.,,~~:: ,".' 

19 412.97> 368.98 

19 412.97> 168.80 

21 414.97> 369 .... 

21 420.97> 375 .... 

416.97> 371.9 

min 

Rr .. >: 

7.11 

7.11 

7.11 

7.11 

7.11 

13C2·PFOA 

FC2G_1508025 

100 

% 

0 

7.00 

.. ~~,:I·;< 

71271 

14476 

76943 

45963 

47055 

7.11 

7.69e4 

308258 

8.00 

:':I~~~I;1· 

76943 

76943 

47055 

47055 

min 

:ljIg'<:: 

71.234 

62.738 

42.203 

11.456 

9.832 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Conen & LOD units = ng abs. 

13C8·PFOA 

FC2G _1508025 

7.00 

1i:;;LOD 

0.6616 

7.11 

"4.60e4 

182882 

I 

8.00 

~,,~. 

56.9 

92.6 

79.5 

min 

:SlN;:: 

0 

0 

194 

0 

0 

13C4·PFOA 

FC2G 1508025 

100 - 7.11 

4.71e4 

186060 

% 

0 min 

7.00 8.00 

'·':NoiSe". 'f;elQht ' " I.S~I!jht 

0 276730 308258 

0 55133 308258 

1590 308258 186060 

0 182882 186060 

0 186060 

,;~~~es~: Fsc:;I\1r,l/c:O~~ctiOn;;; 

0.9700 1.0 MM 

0.9700 1.0 bb 

0.9700 1.0 bb 

0.9700 1.0 MM 

0.9700 1.0 MM 

www.axysanalytical.com Page 62 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRo\FC\FC2G _150-b.qld 

Last Altered: Friday, April 27, 2012 11:33:53 Pacific Daylight Time 

Printed: Friday, April 27, 2012 13:05:43 Pacific Daylight Time 

10: WG39723·103"DUP 

Name: FC2G_150S026 

Description: 1 ,WG39723,15/4000uL 

Date: 19·Apr-2012 

Time: 03:33:47 

PFOA·1 PFOA-2 

FC2G _150S026 

13C2·PFOA 

FC2G_150S026 

100 7.11 

1.57e4 

61141 

10 7.11 

8.98e4 

365752 

% 

o 

7.00 8.00 7.00 8.00 

5 PFOA-1 19 412.97> 368.98 7.11 72452 89792 

6 PFOA-2 19 412.97> 168.80 7.11 15695 89792 

19 13C2-PFOA 21 414.97>369 .... 7.11 89792 45129 

20 13C8-PFOA 21 420.97 > 375 .... 7.11 49612 45129 

21 13C4-PFOA 416.97> 371.9 7.11 45129 

60.787 

57.044 

50.315 

12.633 

9.239 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Concn & LOD units = ng abs. 

13C8-PFOA 

FC2G _150S026 

100 

% 

o 

1.1148 

0.1527 

0.0399 

7.11 

4.96e4 

201798 

8.00 

%~eC·· 

69.2 

104.2 

76.2 

min 

.$11'4.></ 

142 

0 

0 

250 

695 

13C4·PFOA 

FC2G 1508026 

- 7.11 

. Npl$e , 

1939 

0 

0 

808 

262 

4.51e4 

182283 

·~I!:!m 

275184 

61141 

365752 

201798 

182283 

min 

I$~!mt ,\SS",fl!iJ siZe ;F$cI9~Qet~1<!~,,: 

365752 0.9900 1.0 MM 

365752 0.9900 1.0 bb 

182283 0.9900 1.0 bb 

182283 0.9900 1.0 MM 

0.9900 1.0 MM 

www.axysanalytical.com Page 63 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_150-b.qld 

Last Altered: Friday, April 27, 2012 11:33:53 Pacific Daylight Time 

Printed: Friday, April 27, 2012 13:05:43 Pacific Daylight Time 

10: WG39723-101"BLK 

Name: FC2G_150S023 

Description: 1, WG39723, 15/4000uL 

Date: 19-Apr-2012 

Time: 02:43:22 

PFOA-1 

FC2G _1508023 

1001/\ 7.78 

:~m'o 7.00 8.00 

#. 

5 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

PFOA-2 

FC2G _1508023 

100L16'~'11 '\j \,c '.20 

: ., ':( m'o 

7.00 8.00 

19 412.97> 368.98 

19 412.97> 168.80 

13C2-PFOA 

FC2G _1508023 

100 

% 

o 

7.11 

1.96e5 

789308 

196286 

196286 

21 414.97> 369 .... 7.11 196286 61332 80.123 

21 420.97>375 .... 7.11 64527 61332 11.969 

416.97> 371.9 7.11 61332 12.431 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Concn & LOD units = ng abs. 

13C8-PFOA 

FC2G _1508023 

13C4-PFOA 

FC2G_1508023 

100 7.11 

6.45e4 

257648 

% 

7.00 8.00 

;;%~ecY;S/""<2;;; 'N{j/$\i;> 

327 

104 

111.3 0 

99.7 a 

103.6 0 

o 

o 

a 

7.11 

6.13e4 

243292 

;fi~ijli~rf 

789308 

257648 

243292 

ISHlilightr ~{!r\~;siZe: . f'llI~Oelf!clion,;. 

789308 1.0000 1.0 MM-I 

789308 1.0000 1.0 

243292 1.0000 1.0 MM 

243292 1.0000 1.0 MM 

1.0000 1.0 MM 

www.axysanalytical.com Page 64 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: 

Last Altered: 

Printed: 

G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G _150-b.qld 

Friday, April 27, 201211:33:53 Pacific DaylightTime 

Friday, April 27, 2012 13:05:43 Pacific Daylight Time 

ID: WG39723-102"SPM 

Name: FC2G_150S019 

Description: 1,WG39723,15/4000uL 

Date: 19-Apr-2012 

Time: 01:36:19 

PFOA-1 PFOA-2 

FC2G_1508019 FC2G_1508019 

100 

% 

7.11 

8.47e4 

340427 

100 

% 

7.11 

2.03e4 

80525 

13C2-PFOA 

FC2G_150S019 

100 

% 

7.11 

1.48e5 

605508 

o -4TPI"T'Frr'-' min o o 'i9'i'"Fl-1,..,.-n min 

7.00 8.00 7.00 8.00 

.-IS Altifa.- ~:rJtJ/;~ • 

5 19 412.97> 368.98 7.11 84691 148381 42.544 

6 PFOA-2 19 412.97> 168.80 7.11 20261 148381 43.918 

19 13C2-PFOA 21 414.97> 369 .... 7.11 148381 52535 70.711 

20 13C8-PFOA 21 420.97> 375 .... 7.11 55059 52535 11.923 

21 13C4-PFOA 416.97> 371.9 7.11 52535 10.648 

PV by HH, SV by JP 27-Apr-12, NOTE: CAUVERlSPM Concn & LOD units = ng abs. 

13C8-PFOA 

FC2G _1508019 

100 

% 

o 

0.1477 

7.11 

5.51e4 

227324 

106.4 

109.8 

98.2 

99.4 

88.7 

543 

383 

0 

0 

0 

13C4-PFOA 

FC2G_150S019 

100 

% 

o 

7.00 

7.11 

5.25e4 

207780 

8.00 

NQiSli ':IEiigh~ ··..\I~H,~i!ltit;~~p'e siZEI; ,.,ac!Qt2Del~tlO!i:;; 

626 340427 605508 1.0000 1.0 MM 

210 

o 

o 

o 

80525 605508 1.0000 

605508 207780 1.0000 

227324 207780 1.0000 

. 207780 1.0000 

1.0 bb 

1.0 MM 

1.0 MM 

1.0 MM 

www.axysanalytical.com Page 65 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G _150-b.qld 

Last Altered: Friday, April 27, 2012 11 :33:53 Pacific Daylight Time 

Printed: Friday, April 27, 2012 13:05:43 Pacific Daylight Time 

ID: WG39723-104"MS 

Name: FC2G_150S027 

Description: 1,WG39723,15/4000uL 

Date: 19-Apr-2012 

Time: 03:50:25 

PFOA-1 

FC2G_150S027 

7.11 

7.70e4 

297089 

7.00 

5 PFOA-1 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

8.00 

PFOA-2 

FC2G_150S027 

13C2-PFOA 

FC2G _150S027 

7.11 

1.58e4 

62539 

19 412.97> 368.98 7.11 

100 

% 

o 7.00 

1·9 412.97> 168.80 7.11 15791 

21 414.97> 369 .... 7.11 93560 

21 420.97> 375 .... 7.11 46797 

416.97> 371.9 7.11 42847 

7.11 

9.36e4 

379945 

8.00 

93560 63.299 

93560 56.186 

42847 56.357 

42847 12.809 

8.953 

PV by HH, SV by JP 27-Apr-12, NOTE: CAUVERlSPM Concn & LaD units = ng abs. 

13C8-PFOA 

FC2G.:.150S027 

7.11 

4.68e4 

189718 

2456.0 0 

2180.0 194 

75.9 0 

103.5 0 

72.4 0 

-----------~.~~ 

13C4-PFOA 

7.00 8.00 

o 297089 379945 

322 

o 

o 

o 

62539 379945 

379945 169876 

189718 169876 

169876 

S!l!l)lllllslZe. Fa¢!~ip.I!I~~~i; 

0.9700 1.0 bb 

0.9700 1.0 bb 

0.9700 1.0 MM 

0.9700 1.0 MM 

0.9700 1.0 MM 

www.axysanalytical.com Page 66 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G_150-b.qld 

Last Altered: Friday, April 27, 2012 11 :33:53 Pacific Daylight Time 

Printed: Friday, April 27, 201213:05:43 Pacific Daylight Time 

ID: WG39723-105"MS 

Name: FC2G_150S028 

Description: 1 ,WG39723, 15/4000uL 

Date: 19-Apr-2012 

Time: 04:07:11 

PFOA-1 

min 

1#' " .. N!!nte 

5 PFOA-l 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

PFOA-2 

FC2G _150S028 

....... 

100 

% 

a 

7.11 

2.71e4 

109534 

7.00 8.00 

'1St/! T~CI!. 

19 412.97> 368.98 

19 412.97> 168.80 

21 414.97> 369 .... 

21 420.97 > 375 .... 

416.97> 371.9 

min 

,Rr-; 

7.11 

7.11 

7.11 

7.11 

7.11 

13C2-PFOA 

FC2G _150S028 

At .. a::;· " 

123944 

27053 

87601 

44188 

42097 

7.11 

8.76e4 

352574 

I\SMa' . 

87601 

87601 

42097 

42097 

min 

nglg" 

108.852 

103.580 

53.709 

12.311 

8.796 

13C8-PFOA 

FC2G _150S028 

100 

% 

a 7.00 

" .. ;J,,~p 

0.1353 

0.0869 

7.11 

4.42e4 

173025 

" 'I 

8.00 

·.%Rec 

264.0 

251.2 

72.4 

99.5 

71.1 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Concn & LOD units = ng abs. 

13C4-PFOA 

min 0 min 

7.00 8.00 

~/II!~ .. Noiss:";·:: H!ilig~t' \~ti~ighl Slimplesize fiat:!9i'2· PetectiOn ... 

0 0 477539 352574 0.9700 1.0 MM 

0 0 109534 352574 0.9700 1.0 bb 

1219 289 352574 165594 0.9700 1.0 bb 

0 0 173025 165594 0.9700 1.0 MM 

303 546 165594 0.9700 1.0 MM 

www.axysanalytical.com Page 67 of 102
Quantify Sample Report MassLynx 4;1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G_150-b.qld 

Last Altered: Friday, April 27, 2012 11 :33:53 Pacific Daylight Time 

Printed: Friday, April 27, 2012 13:05:43 Pacific Daylight Time ----------.~~-----------------------------------------------------

10: WG39723-106"MS 

Name: FC2G_150S029 

Description: 1,WG39723,15/4000ul 

Date: 19·Apr·2012 

Time: 04:23:57 

PFOA·1 

FC2G_150S029 

100 7.11 

9.86e5 

3903333 

7.00 

5 PFOA-l 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

8.00 

PFOA·2 

FC2G _150S029 

100 7.11 

2.55e5 

1049001 

7.00 8.00 " 

[S'" 'ria~ii 

19 412.97> 368.98 

19 412.97> 168.80 

21 414.97> 369 .... 

21 420.97 > 375 .... 

416.97> 371.9 

min 

7.11 

7.11 

7.11 

7.11 

7.11 

13C2·PFOA 

FC2G _150S029 

986435 

255397 

71088 

34512 

33981 

7.11 

7.11e4 

288374 

ISl,Velf 

71088 

71088 

33981 

33981 

min 

13C8·PFOA 

FC2G_150S029 

100 

% 

0 

7.00 

7.11 

3.45e4 

136866 

8.00 

"f19'Q{ · •.. ,LOO. 

1036.274 

1178.492 117.8 

52.374 72.7 

11.554 96.3 

6.887 0.1033 57.4 

PV by HH, SV by JP 27-Apr-12, NOTE: CALNERlSPM Concn & LOD units = ng abs. 

min 

0 

0 

0 

0 

200 

13C4·PFOA 

FC2G 1508029 

100 - 7.11 

3.40e4 

136817 

% 

0 

7.00 8.00 

.• l:lE!Igh,~ 

3903333 

0 1049001 

0 288374 

0 136866 

684 136817 

min 

IS,:!aiQh,!' Sal:llPle!llzB 

288374 1.0000 

288374 1.0000 

136817 1.0000 

136817 1.0000 

1.0000 

"f'aCfor20electiGn,;, 

1.0 MM 

1.0 bb 

1.0 bb 

1.0 MM 

1.0 MM 

www.axysanalytical.com Page 68 of 102
Sample List Report 

4-;; g 0 ff-e1r 6:~ -1' (S~\,~S: ~_~~ _____ ~_" __ ~" MassLynx 4.1 SCN627 

Sample List: G:\Masslynx\lnst_ G\_Master.PRO\SampleOB\FC2G_150.SPL Page 1 of 1 

Last Modified: Wednesday, April 18, 2012 15:12:44 Pacific Daylight Time 

Printed: Wednesday, April 18, 2012 15:15:13 PacificOaylightTime Page Position (1, 1) 

File Name Sample ID File Text Bottle Signln SignOut Inj VoL 

1 FC2G 150S001 MeOH" 1,,15uL 1:8,A ltft 'w' 15.0 

2 FC2G:::150S002 MeOH" 1 ,,15uL 1:8,A 15.0 

3 FC2G 150S003 FC-LlN-39725T-A" 1/2 1" 15uL 2:1,A 15.0 

4 FC2G -150S004 FC-LlN-39725T-A" 1/2 1" 15uL 2:1,A 15.0 

5 FC2G:::150S005 FC-LlN-39725T-B" 1/2 1" 15uL 2:1,8· 15.0 

6 FC2G 150S006 FC-LlN-39725T-C" 1/2 1,,15uL 2:1,C 15.0 

7 FC2G-150S007 FC-LlN-39725T-D" 1/2 1 ,,15uL 2:1,0 15.0 

8 FC2G:::150S008 FC-LlN-39725T-E" 1/2 1" 15uL 2:1,E 15.0 ';2(!;)t, .J '<iJ 

9 FC2G 150S009 FC-LlN-39725T-F" 1/2 1 ,,15uL 2:1,F 15.0 

10 FC2G:::150S010 FC-LlN-39725T-G" 1/2 1" 15uL 2:2,A 15.0 

11 FC2G 150S011 FC-LlN-39725T-H" 1/2 1" 15uL 2:2,8 15.0· 

12 FC2G:::150S012 MeOH" 1,,15uL 1:8,A 15.0 

13 FC2G 150S013 MeOH" 1" 15uL 1:8,A 15.0 

14 FC2G-150S014 FC-39725T-SAR2" 1" 15uL 2:2,C 15.0~ 

15 FC2G-150S015 FC-39725T-SAR" 1" 15uL 2:2,0 15.0 

16 FC2G-150S016 FC-LlN-39725T-D" 1/2 1,,15uL 2:1,0 15.0 

17 FC2G -150S017 FC-LlN-39725T-~ 1/2 1 ,,15uL 2:1,D 15.0 

18 FC2G-150S018 WG39723-1.Q3::S R ~~G39723,15/4000UL 2:2,E 15.0 

19 FC2G:::150S019 WG39723-102"SPM ,G39723, 15/4000uL 2:2,F 15.0 

20 FC2G 150S020 WG39723-102,i,SPM 1,WG39723,15/4000uL 2:2,F 15.0 

21 FC2G-150S021 MeOH" 1" 15uL 1 :8,A 15.0 

22 F C2G::: 150S022 MeOH" 1" 15uL 1 :8,A 15.0 

23 FC2G_150S023 WG39723-101 "BLK 1,WG39723,15/4000uL 2:3,A 15.0 r;t}~, ... " \ I:;<?" V~ 

24 FC2G_150S024 WG39723-101,i,BLK 1 ,WG39723,15/4000uL 2:3,A 15.0 

25 FC2G 150S025 L 17643-1" 1,WG39723,15/4000uL 2:3,8 15.0 

26 F C2G -150S026 WG39723-103"DUP 1,WG39723,15/4000uL 2:3,C 15.0 

27 F C2G -150S027 WG39723-104"MS 1,wG39723,15/4000uL 2:3,0 15.0 

28 FC2G:::150S028 WG39723-105"MS 1,wG39723,15/4000uL 2:3,E 15.0 

29 FC2G 150S029 WG39723-106"MS 1,wG39723,15/4000uL 2:3,F 15.0 

30 FC2G-150S030 MeOH" 1;,15uL 1:8,A 15.0 

31 FC2G-150S031 FC-LlN-39725T-D" 1/2 1" 15uL 2:1,0 15.0J 

32 FC2G:::150S032 FC-LlN-39725T-D" 1/2 1,,15uL 2:1,D 15.0 

33 FC2G 150S033 MeOH" 1,,15uL 1 :8,A 15.0 

34 FC2G:::150S034 MeOH" 1,,15uL 1:8,A -- 15.0 

www.axysanalytical.com Page 69 of 102
Quantify Compound Summary Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeMethod: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 08 Nov 2011 13:21:58Calibration: G:\Masslynx\Inst_G\PFC.PRO\CurveDB\FC2G_150-A1.cdb 19 Apr 2012 11:51:33 Compound name: PFOA-1

#12345678

IDFC-LIN-39725T-A,,1/2FC-LIN-39725T-B,,1/2FC-LIN-39725T-C,,1/2FC-LIN-39725T-D,,1/2FC-LIN-39725T-E,,1/2FC-LIN-39725T-F,,1/2FC-LIN-39725T-G,,1/2FC-LIN-39725T-H,,1/2

NameFC2G_150S004FC2G_150S005FC2G_150S006FC2G_150S007FC2G_150S008FC2G_150S009FC2G_150S010FC2G_150S011

TypeStandardStandardStandardStandardStandardStandardStandardStandard

RT7.157.157.117.157.117.117.117.11

Area

236881062755910118347612595803217311153196280

IS Area175916197721203449173989182498171454137030113640

Std. Conc1.0002.50010.00040.000200.000400.0001000.0002500.000

ng/g0.9212.97810.03343.349194.766417.235943.4252100.550

%Rec92.1119.1100.3108.497.4104.394.384.0

S/N5825066700000

Acq.Date

18-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-12

Acq.Time

21:24:4621:41:3121:58:1622:15:0222:31:4822:48:3423:05:2023:22:06

Compound name: PFOA-1Correlation coefficient: r = 0.993303, r^2 = 0.986650Calibration curve: 0.96404 * x + 0.0810483Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area )Curve type: Linear, Origin: Exclude, Weighting: 1/x^2, Axis trans: None

Conc

0 500 1000 1500 2000 2500

Response

0

500

100015002000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Compound Summary Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeCompound name: PFOA-2

#12345678

IDFC-LIN-39725T-A,,1/2FC-LIN-39725T-B,,1/2FC-LIN-39725T-C,,1/2FC-LIN-39725T-D,,1/2FC-LIN-39725T-E,,1/2FC-LIN-39725T-F,,1/2FC-LIN-39725T-G,,1/2FC-LIN-39725T-H,,1/2

NameFC2G_150S004FC2G_150S005FC2G_150S006FC2G_150S007FC2G_150S008FC2G_150S009FC2G_150S010FC2G_150S011

TypeStandardStandardStandardStandardStandardStandardStandardStandard

RT7.157.157.117.157.117.117.117.11

Area

10731690659224314114395229684420193796965

IS Area175916197721203449173989182498171454137030113640

Std. Conc1.0002.50010.00040.000200.000400.0001000.0002500.000

ng/g1.0951.8999.73044.968204.867438.8601005.7302301.323

%Rec109.576.097.3112.4102.4109.7100.692.1

S/N1417534990000

Acq.Date

18-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-12

Acq.Time

21:24:4621:41:3121:58:1622:15:0222:31:4822:48:3423:05:2023:22:06

Compound name: PFOA-2Correlation coefficient: r = 0.991804, r^2 = 0.983675Calibration curve: 0.219327 * x + 0.198982Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area )Curve type: Linear, Origin: Exclude, Weighting: 1/x^2, Axis trans: None

Conc

0 500 1000 1500 2000 2500

Response

0

200400

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Compound Summary Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeCompound name: 13C2-PFOA

#12345678

IDFC-LIN-39725T-A,,1/2FC-LIN-39725T-B,,1/2FC-LIN-39725T-C,,1/2FC-LIN-39725T-D,,1/2FC-LIN-39725T-E,,1/2FC-LIN-39725T-F,,1/2FC-LIN-39725T-G,,1/2FC-LIN-39725T-H,,1/2

NameFC2G_150S004FC2G_150S005FC2G_150S006FC2G_150S007FC2G_150S008FC2G_150S009FC2G_150S010FC2G_150S011

TypeStandardStandardStandardStandardStandardStandardStandardStandard

RT7.157.157.117.117.117.117.117.11

Area

175916197721203449173989182498171454137030113640

IS Area6352170067717026268762790579584824636680

Std. Conc72.00072.00072.00072.00072.00072.00072.00072.000

ng/g69.33470.64871.03669.48672.76574.06171.10877.563

%Rec96.398.198.796.5101.1102.998.8107.7

S/N00000000

Acq.Date

18-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-12

Acq.Time

21:24:4621:41:3121:58:1622:15:0222:31:4822:48:3423:05:2023:22:06

Compound name: 13C2-PFOAResponse Factor: 0.479319RRF SD: 0.0182766, % Relative SD: 3.81302Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area )Curve type: RF

Conc

0.0 10.0 20.0 30.0 40.0 50.0 60.0 70.0

Response

0.0

10.020.030.0

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Compound Summary Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeCompound name: 13C8-PFOA

#12345678

IDFC-LIN-39725T-A,,1/2FC-LIN-39725T-B,,1/2FC-LIN-39725T-C,,1/2FC-LIN-39725T-D,,1/2FC-LIN-39725T-E,,1/2FC-LIN-39725T-F,,1/2FC-LIN-39725T-G,,1/2FC-LIN-39725T-H,,1/2

NameFC2G_150S004FC2G_150S005FC2G_150S006FC2G_150S007FC2G_150S008FC2G_150S009FC2G_150S010FC2G_150S011

TypeStandardStandardStandardStandardStandardStandardStandardStandard

RT7.117.157.117.117.117.117.117.11

Area

6829171880774726395266374607485329137672

IS Area6352170067717026268762790579584824636680

Std. Conc12.00012.00012.00012.00012.00012.00012.00012.000

ng/g12.23111.67112.29211.60612.02611.92412.56611.684

%Rec101.997.3102.496.7100.299.4104.797.4

S/N02473924187206127620

Acq.Date

18-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-12

Acq.Time

21:24:4621:41:3121:58:1622:15:0222:31:4822:48:3423:05:2023:22:06

Compound name: 13C8-PFOAResponse Factor: 1.05481RRF SD: 0.0302495, % Relative SD: 2.86777Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area )Curve type: RF

Conc

0.0 2.0 4.0 6.0 8.0 10.0 12.0

Response

0.05.0

10.0

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Compound Summary Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeCompound name: 13C4-PFOA

#12345678

IDFC-LIN-39725T-A,,1/2FC-LIN-39725T-B,,1/2FC-LIN-39725T-C,,1/2FC-LIN-39725T-D,,1/2FC-LIN-39725T-E,,1/2FC-LIN-39725T-F,,1/2FC-LIN-39725T-G,,1/2FC-LIN-39725T-H,,1/2

NameFC2G_150S004FC2G_150S005FC2G_150S006FC2G_150S007FC2G_150S008FC2G_150S009FC2G_150S010FC2G_150S011

TypeStandardStandardStandardStandardStandardStandardStandardStandard

RT7.117.157.117.117.117.117.117.11

Area

6352170067717026268762790579584824636680

IS Area Std. Conc

12.00012.00012.00012.00012.00012.00012.00012.000

ng/g12.87414.20114.53312.70612.72611.7479.7787.434

%Rec107.3118.3121.1105.9106.197.981.562.0

S/N0297123299463405500

Acq.Date

18-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-1218-Apr-12

Acq.Time

21:24:4621:41:3121:58:1622:15:0222:31:4822:48:3423:05:2023:22:06

Compound name: 13C4-PFOAResponse Factor: 4933.87RRF SD: 969.161, % Relative SD: 19.643Response type: External Std, AreaCurve type: RF

Conc

0.0 2.0 4.0 6.0 8.0 10.0 12.0

Response

0

200004000060000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeMethod: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 08 Nov 2011 13:21:58Calibration: G:\Masslynx\Inst_G\PFC.PRO\CurveDB\FC2G_150-A1.cdb 19 Apr 2012 11:51:33 ID: FC-LIN-39725T-A,,1/2Name: FC2G_150S004Description: 1,,15uLDate: 18-Apr-2012Time: 21:24:46PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S004

PFOA-1

7.15

2.37e3

9475

7.78

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S004

PFOA-2

7.15

1.07e3

3700

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S004

13C2-PFOA

7.15

1.76e5

661148

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S004

13C8-PFOA

7.11

6.83e4

268861

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S004

13C4-PFOA

7.11

6.35e4

249525

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.157.157.157.117.11

Area

236810731759166829163521

IS Area1759161759166352163521

ng/g0.9211.09569.33412.23112.874

LOD %Rec

92.1109.596.3101.9107.3

S/N5814000

Noise163257000

Height94753700661148268861249525

IS Height661148661148249525249525

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-B,,1/2Name: FC2G_150S005Description: 1,,15uLDate: 18-Apr-2012Time: 21:41:31PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S005

PFOA-1

7.15

8.11e3

31540

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S005

PFOA-2

7.15

1.69e3

6900

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S005

13C2-PFOA

7.15

1.98e5

802003

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S005

13C8-PFOA

7.15

7.19e4

283919

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S005

13C4-PFOA

7.15

7.01e4

279051

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.157.157.157.157.15

Area

810616901977217188070067

IS Area1977211977217006770067

ng/g2.9781.89970.64811.67114.201

LOD0.01400.1436

%Rec119.176.098.197.3118.3

S/N2501702473297

Noise1264000115940

Height315406900802003283919279051

IS Height802003802003279051279051

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-C,,1/2Name: FC2G_150S006Description: 1,,15uLDate: 18-Apr-2012Time: 21:58:16PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S006

PFOA-1

7.11

2.76e4

109260

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S006

PFOA-2

7.11

6.59e3

24907

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S006

13C2-PFOA

7.11

2.03e5

789272

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S006

13C8-PFOA

7.11

7.75e4

308429

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S006

13C4-PFOA

7.11

7.17e4

275873

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.117.117.117.117.11

Area

2755965922034497747271702

IS Area2034492034497170271702

ng/g10.0339.73071.03612.29214.533

LOD0.04130.0354

%Rec100.397.398.7102.4121.1

S/N6675309241232

Noise1644670334224

Height10926024907789272308429275873

IS Height789272789272275873275873

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-D,,1/2Name: FC2G_150S007Description: 1,,15uLDate: 18-Apr-2012Time: 22:15:02PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S007

PFOA-1

7.15

1.01e5

393798

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S007

PFOA-2

7.15

2.43e4

93390

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S007

13C2-PFOA

7.11

1.74e5

670969

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S007

13C8-PFOA

7.11

6.40e4

252187

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S007

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.157.157.117.117.11

Area

101183243141739896395262687

IS Area1739891739896268762687

ng/g43.34944.96869.48611.60612.706

LOD0.01930.0384

%Rec108.4112.496.596.7105.9

S/N049901872994

Noise01870135239

Height39379893390670969252187237761

IS Height670969670969237761237761

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-E,,1/2Name: FC2G_150S008Description: 1,,15uLDate: 18-Apr-2012Time: 22:31:48PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S008

PFOA-1

7.11

4.76e5

1953338

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S008

PFOA-2

7.11

1.14e5

469627

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S008

13C2-PFOA

7.11

1.82e5

746660

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S008

13C8-PFOA

7.11

6.64e4

272473

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S008

13C4-PFOA

7.11

6.28e4

252845

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.117.117.117.117.11

Area

4761251143951824986637462790

IS Area1824981824986279062790

ng/g194.766204.86772.76512.02612.726

LOD0.0603

%Rec97.4102.4101.1100.2106.1

S/N0000634

Noise0000399

Height1953338469627746660272473252845

IS Height746660746660252845252845

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-F,,1/2Name: FC2G_150S009Description: 1,,15uLDate: 18-Apr-2012Time: 22:48:34PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S009

PFOA-1

7.11

9.58e5

3865211

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S009

PFOA-2

7.11

2.30e5

937944

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S009

13C2-PFOA

7.11

1.71e5

706324

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S009

13C8-PFOA

7.11

6.07e4

244070

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S009

13C4-PFOA

7.11

5.80e4

242971

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.117.117.117.117.11

Area

9580322296841714546074857958

IS Area1714541714545795857958

ng/g417.235438.86074.06111.92411.747

LOD0.0560

%Rec104.3109.7102.999.497.9

S/N0006120

Noise0003990

Height3865211937944706324244070242971

IS Height706324706324242971242971

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-G,,1/2Name: FC2G_150S010Description: 1,,15uLDate: 18-Apr-2012Time: 23:05:20PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S010

PFOA-1

7.11

1.73e6

7293368

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S010

PFOA-2

7.11

4.20e5

1787472

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S010

13C2-PFOA

7.11

1.37e5

575308

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S010

13C8-PFOA

7.11

5.33e4

223856

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S010

13C4-PFOA

7.11

4.82e4

201739

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.117.117.117.117.11

Area

17311154201931370305329148246

IS Area1370301370304824648246

ng/g943.4251005.73071.10812.5669.778

LOD0.04970.0533

%Rec94.3100.698.8104.781.5

S/N000762550

Noise000294367

Height72933681787472575308223856201739

IS Height575308575308201739201739

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
Quantify Sample Report MassLynx 4.1 SCN627

Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_150-A1.qldLast Altered: Thursday, April 19, 2012 11:51:33 Pacific Daylight TimePrinted: Thursday, April 19, 2012 12:55:08 Pacific Daylight TimeID: FC-LIN-39725T-H,,1/2Name: FC2G_150S011Description: 1,,15uLDate: 18-Apr-2012Time: 23:22:06PFOA-1

min

7.00 8.00

%

0

100

FC2G_150S011

PFOA-1

7.11

3.20e6

12985217

PFOA-2

min

7.00 8.00

%

0

100

FC2G_150S011

PFOA-2

7.11

7.97e5

3272521

13C2-PFOA

min

7.00 8.00

%

0

100

FC2G_150S011

13C2-PFOA

7.11

1.14e5

462634

13C8-PFOA

min

7.00 8.00

%

0

100

FC2G_150S011

13C8-PFOA

7.11

3.77e4

156104

13C4-PFOA

min

7.00 8.00

%

0

100

FC2G_150S011

13C4-PFOA

7.11

3.67e4

150361

#56192021

NamePFOA-1PFOA-213C2-PFOA13C8-PFOA13C4-PFOA

IS#19192121

Trace412.97> 368.98412.97> 168.80414.97 > 369....420.97 > 375....416.97 > 371.9

RT7.117.117.117.117.11

Area

31962807969651136403767236680

IS Area1136401136403668036680

ng/g2100.5502301.32377.56311.6847.434

LOD %Rec

84.092.1107.797.462.0

S/N00000

Noise00000

Height129852173272521462634156104150361

IS Height462634462634150361150361

Samp1.0001.0001.0001.0001.000

PV by HH, Checked by JP 19-Apr-12, NOTE: CAL/VER Concn & LOD units = ng abs.

www.axysanalytical.com
---'--'-------------------------------------------------------------

Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: 

Last Altered: 

Printed: 

G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G _150-b.qld 

Friday, April 27, 2012 11 :33:53 Pacific DaylightTime 

Friday, April 27, 201213,:05:43 Pacific Daylight Time 

Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 08 Nov 2011 13:21 :58 

Calibration: G:\Masslynx\lnsC G\PFC.PRO\CurveDB\FC2G_150-A1.cdb 19 Apr 2012 11 :51 :33 

ID: FC-LlN-39725T -D" 1/2 

Name: FC2G_150S016 

Description: 1,,15uL 

Date: 19-Apr-2012 

Time: 00:46:01 

PFOA-1 

FC2G_150S016 

100 7.11 

9.97e4 

395087 

7.00 8.00 " 

, min 

PFOA-2 

FC2G_150S016 

100 7.11 

2.47e4 

100227 

% 

o 7.00 8.00 

13C2-PFOA 

FC2G_150S016 

100 7.11 

'~8~~~~ % >.,~ttl 

o , "" ,min 

7.00 8.00 

13C8-PFOA 

7.00 8.00 " 

# ,'Area," -J$Arjfjil '.~g;, ':',o/.Ree,' 

5 PFOA-1 19 412.97> 368.98 7.11 99677 173449 42.836 107.1 

6 PFOA-2 19 412.97> 168.80 7_11 24700 173449 45.842 114.6 

19 13C2-PFOA 21 414.97 > 369 __ .. 7.11 173449 65286 66.513 0.0816 92.4 

20 13C8-PFOA 21 420.97 > 375 .... 7.11 68008 65286 11.851 98.8 

21 13C4-PFOA 416.97> 371.9 7.11 65286 13.232 110.3 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Concn & LOD units = ng abs. 

13C4-PFOA 

FC2G_150S016 

100 7.11 

% 

min 0- min 

7.00 8.00 

,~lN·:. No!ile H~igbt . ",;1$ 11&lgl:lt '. Siilll'ij)Iesiz& :'!"actor;!OetectlOn; .. 

0 0 395087 681240 1.0000 1.0 MM 

849 118 100227 681240 1.0000 1,0 bb 

2373 287 681240 264379 1.0000 1.0 MM 

0 0 270350 264379 1.0000 1.0 MM 

0 0 264379 1.0000 1.0 MM 

www.axysanalytical.com Page 83 of 102
Quantify Sample Report MassLynx 4.1 SCN627 

Dataset: G:\Masslynx\lnst_ G\PFC.PRO\FC\FC2G _150-b.qld 

Last Altered: Friday, April 27, 2012 11 :33:53 Pacific Daylight Time 

Printed: Friday, April 27, 2012 13:05:43 Pacific Daylight Time ------~~--------------------------------------------------------------------------

10: FC-LlN·39725T ·0" 1/2 

Name: FC2G_150S031 

Description: 1,,15uL 

Date: 19-Apr·2012 

Time: 04:57:29 

PFOA-1 

#~. ",elM:;; 

5 PFOA-1 

6 PFOA-2 

19 13C2-PFOA 

20 13C8-PFOA 

21 13C4-PFOA 

PFOA·2 

FC2G_150S031 

100 7.11 

2.50e4 

100798 

% 

o ~h-r-~ min 

7.00 B.OO 

"!$# I~T~ 

19 412.97>368.98 7.11 

19 412.97> 168.80 7.11 

21 414.97> 369 .... 7.11 

21 420.97 > 375 .... 7.11 

416.97> 371.9 7.11 

13C2-PFOA 

FC2G _150S031 

100 . 7.11 ., 1.77e5 

; 738968 

% 

o 

7.00 

~f"iT-rTl~ min 

8.00 

101726 177273 42.774 

25025 177273 45.435 

177273 62325 71.210 

69626 62325 12.709 

62325 12.632 

PV by HH, SV by JP 27-Apr-12, NOTE: CALlVERlSPM Concn & LOD units = ng abs. 

13C8·PFOA 

FC2G_150S031 

100 7.11 

% 

7.00 B.OO 

0.0025 106.9 

0.6496 113.6 

98.9 

0.0683 105.9 

0.0291 105.3 

1373 

86 

0 

574 

1304 

13C4·PFOA 

FC2G_150S031 

100 7.11 

6.23e4 

245393 

'" , min 

7.00 8.00 

N~~:' '!'l~I!l~~ .•. ISHeil/fit i~mple!!iaeiF!ilCt()~::I)III!1ctlon,,; 

286 392055 738968 1.0000 1.0 MM 

1168 

o 

491 

188 

100798 738968 1.0000 

738968 245393 1.0000 

281857 245393 1.0000 

245393 1.0000 

1.0 bb 

1.0 MM 

1.0 MM 

1.0 MM 

www.axysanalytical.com Page 84 of 102
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

Table 1a 

NELAP Accreditation Held by AXYS Analytical Services Ltd. 

 for Chlorinated Dioxins/Furans, Chlorinated Pesticides, PCBs and PAHs

Matrix Codes for Table 1a NPW = Non-Potable Water DrW = Drinking WaterS = Solid T = Tissue Accreditation Method Codes and Explanation for Table 1 Code Accreditation Certificate Applicable AXYS Method and Description

No. Method Reference

1 EPA 1613B MLA-017, performance based implementation of EPA1613B (GC/HRMS) 

2 EPA 8290 MLA-017, performance based implementation of EPA 8290 (GC/HRMS) 3 AXYS MLA-017 MLA-017, performance based implementation of EPA 1613B, 8290 (GC/HRMS) 

4 EPA 608 MLA-007, performance based implementation of EPA 608 (GC/ECD) 

5 EPA 8270C or 8270D MLA-007, performance based modification of 8270C/D (GC/LRMS) 

6 EPA 8081A or 8081B MLA-007, performance based implementation of EPA 8081A/B (GC/ECD) 7 EPA 1668A MLA-010, performance based implementation of EPA 1668A (GC/HRMS) 8 SM 6630B MLA-007, performance based implementation of SM 18-20 6630B (GC/ECD) 9 EPA 1625B MLA-021, performance based modification of EPA 1625B (GC/LRMS) 

11 EPA 625 MLA-007, performance based modification of EPA 625 (GC/LRMS) 

20 EPA 8270C or 8270D MLA-021, performance based modification of EPA 8270C/D (GC/LRMS) 

New York 

State 

Department 

of Health 

California 

Department 

of Public 

Health 

State of Florida 

Department 

of Health 

State of New 

Departm

Environm

Protec

Lab ID 

11674 

NELAP 

Primary 

Lab ID 

01138CA 

NELAP 

Secondary 

Lab ID E871007 

NELAP Primary 

Lab ID CA

NELAP Se

TABLE 1 

NP

W S NPW S Dr. W NP W S T Dr. W NP W 

PCDD/F - Polychlorinated Dioxins and Furans Dioxins 1 Dioxins and Dibenzofurans 2 1,2,3,4,6,7,8-HpCDD 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,4,6,7,8-HpCDF 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,4,7,8,9-HpCDF 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,4,7,8-HxCDD 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,4,7,8-HxCDF 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,6,7,8-HxCDD 1 2 1, 2, 3 2, 3 2, 3 1 

www.axysanalytical.com
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

New York 

State 

Department 

of Health 

California 

Department 

of Public 

Health 

State of Florida 

Department 

of Health 

State of New 

Departm

Environm

Protec

Lab ID 

11674 

NELAP 

Primary 

Lab ID 

01138CA 

NELAP 

Secondary 

Lab ID E871007 

NELAP Primary 

Lab ID CA

NELAP Se

TABLE 1 

NP

W S NPW S Dr. W NP W S T Dr. W NP W 

1,2,3,6,7,8-HxCDF 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,7,8,9-HxCDD 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,7,8,9-HxCDF 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,7,8-PeCDD 1 2 1, 2, 3 2, 3 2, 3 1 1,2,3,7,8-PeCDF 1 2 1, 2, 3 2, 3 2, 3 1 2,3,4,6,7,8-HxCDF 1 2 1, 2, 3 2, 3 2, 3 1 2,3,4,7,8-PeCDF 1 2 1, 2, 3 2, 3 2, 3 1 2,3,7,8-TCDD 1 1 2 1, 2, 3 2, 3 2, 3 1 2,3,7,8-TCDF 1 2 1, 2, 3 2, 3 2, 3 1 OCDD 1 2 1, 2, 3 2, 3 2, 3 1 OCDF 1 2 1, 2, 3 2, 3 2, 3 1 Total TCDD 1 1, 2, 3 2, 3 2, 3 Total TCDF 1 1, 2, 3 2, 3 2, 3 Total PeCDD 1 1, 2, 3 2, 3 2, 3 Total PeCDF 1 1, 2, 3 2, 3 2, 3 Total HxCDD 1 1, 2, 3 2, 3 2, 3 Total HxCDF 1 1, 2, 3 2, 3 2, 3 Total HpCDD 1 1, 2, 3 2, 3 2, 3 Total HpCDF 1 1, 2, 3 2, 3 2, 3 PCBs – Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl 7 7 7 PCB 3 4-Chlorobiphenyl 7 7 7 PCB 4 2,2'-Dichlorobiphenyl 7 7 7 PCB 5 2,3-Dichlorobiphenyl 7 7 7 PCB 15 4,4'-Dichlorobiphenyl 7 7 7 PCB 18 2,2',5-Trichlorobiphenyl 7 7 7 PCB 19 2,2',6-Trichlorobiphenyl 7 7 7 PCB 31 2,4',5-Trichlorobiphenyl 7 7 7 PCB 37 3,4,4'-Trichlorobiphenyl 7 7 7 PCB 44 2,2',3,5'-Tetrachlorobiphenyl 7 7 7 PCB 52 2,2',5,5'-Tetrachlorobiphenyl 7 7 7 PCB 54 2,2',6,6'-Tetrachlorobiphenyl 7 7 7 PCB 66 2,3',4,4'-Tetrachlorobiphenyl 7 7 7 PCB 77 3,3',4,4'-Tetrachlorobiphenyl 7 7 7 PCB 81 3,4,4',5-Tetrachlorobiphenyl 7 7 7 PCB 87 2,2',3,4,5'-Pentachlorobiphenyl 7 7 7 

www.axysanalytical.com
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

New York 

State 

Department 

of Health 

California 

Department 

of Public 

Health 

State of Florida 

Department 

of Health 

State of New 

Departm

Environm

Protec

Lab ID 

11674 

NELAP 

Primary 

Lab ID 

01138CA 

NELAP 

Secondary 

Lab ID E871007 

NELAP Primary 

Lab ID CA

NELAP Se

TABLE 1 

NP

W S NPW S Dr. W NP W S T Dr. W NP W 

PCB 101 2,2',4,5,5'-Pentachlorobiphenyl 7 7 7 PCB 104 2,2',4,6,6'-Pentachlorobiphenyl 7 7 7 PCB 105 2,3,3',4,4'-Pentachlorobiphenyl 7 7 7 PCB 109 2,3,3',4,6-Pentachlorobiphenyl 7 7 7 PCB 114 2,3,4,4',5-Pentachlorobiphenyl 7 7 7 PCB 118 2,3',4,4',5-Pentachlorobiphenyl 7 7 7 PCB 123 2,3',4,4',5'-Pentachlorobiphenyl 7 7 7 PCB 124 2,3',4',5,5'-Pentachlorobiphenyl 7 PCB 126 3,3',4,4',5-Pentachlorobiphenyl 7 7 7 PCB 138 2,2',3,4,4',5'-Hexachlorobiphenyl 7 7 7 PCB 141 2,2',3,4,5,5'-Hexachlorobiphenyl 7 7 7 PCB 151 2,2',3,5,5',6-Hexachlorobiphenyl 7 7 7 PCB 153 2,2',4,4',5,5'-Hexachlorobiphenyl 7 7 7 PCB 155 2,2',4,4',6,6'-Hexachlorobiphenyl 7 7 7 PCB 156 2,3,3',4,4',5-Hexachlorobiphenyl 7 7 PCB 157 2,3,3',4,4',5'-Hexachlorobiphenyl 7 7 7 PCB 167 2,3',4,4',5,5'-Hexachlorobiphenyl 7 7 7 PCB 169 3,3',4,4',5,5'-Hexachlorobiphenyl 7 7 7 PCB 170 2,2',3,3',4,4',5-Heptachlorobiphenyl 7 7 7 PCB 180 2,2',3,4,4',5,5'-Heptachlorobiphenyl 7 7 7 PCB 183 2,2',3,4,4',5',6-Heptachlorobiphenyl 7 7 7 PCB 187 2,2',3,4',5,5',6-Heptachlorobiphenyl 7 7 7 PCB 188 2,2',3,4',5,6,6'-Heptachlorobiphenyl 7 7 7 PCB 189 2,3,3',4,4',5,5'-Heptachlorobiphenyl 7 7 7 PCB 202 2,2',3,3',5,5',6,6'-Octachlorobiphenyl 7 7 7 PCB 205 2,3,3',4,4',5,5',6-Octachlorobiphenyl 7 7 7 PCB 206 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 7 7 7 PCB 208 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl 7 7 7 PCB 209 Decachlorobiphenyl 7 7 7 Aroclor 1260 7, 11 5, 7 11 5 Aroclor 1254 7, 11 5, 7 11 5 Aroclor 1221 7, 11 5, 7 11 5 Aroclor 1232 7, 11 5, 7 11 5 Aroclor 1248 7, 11 5, 7 11 5 Aroclor 1016 7, 11 5, 7 11 5 Aroclor 1242 7, 11 5, 7 11 5 

www.axysanalytical.com
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

New York 

State 

Department 

of Health 

California 

Department 

of Public 

Health 

State of Florida 

Department 

of Health 

State of New 

Departm

Environm

Protec

Lab ID 

11674 

NELAP 

Primary 

Lab ID 

01138CA 

NELAP 

Secondary 

Lab ID E871007 

NELAP Primary 

Lab ID CA

NELAP Se

TABLE 1 

NP

W S NPW S Dr. W NP W S T Dr. W NP W 

Pesticides 4,4'-DDD 11 5 11 5 4,4'-DDE 11 5 11 5 4,4'-DDT 11 5 11 5 Aldrin 11 5 11 5 Alpha-HCH 11 5 11 5 Beta-HCH 11 5 11 5 cis-Chlordane (alpha-Chlordane) 5 5 Chlordane, technical 5, 11 5 11 5 Delta-HCH 11 5 11 5 Dieldrin 4 6 4 6 Endosulphan I 4 6 4 6 Endosulphan II 4 6 4 6 Endosulphan sulphate 4 6 4 6 Endrin 4 6 4 6 Endrin aldehyde 4 6 4 6 trans-Chlordane (gamma-Chlordane) 5 5 Gamma-HCH (Lindane) 11 5 11 5 Heptachlor 11 5 11 5 Heptachlor epoxide 4 6 4 6 Hexachlorobenzene 9 5 9 5 Methoxychlor 4,8 6 8 6 Mirex 5 PAH Anthracene 9 20 9 20Pyrene 9 20 9 20Benzo[ghi]perylene 9 20 9 20Indeno[1,2,3-cd]pyrene 9 20 9 20Benzo[b]fluoranthene 9 20 9 20Fluoranthene 9 20 9 20Benzo[k]fluoranthene 9 20 9 20Acenaphthylene 9 20 9 20Chrysene 9 20 9 20Benzo[a]pyrene 9 20 9 20Dibenz[ah]anthracene 9 20 9 20Benz[a]anthracene 9 20 9 20

www.axysanalytical.com
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

New York 

State 

Department 

of Health 

California 

Department 

of Public 

Health 

State of Florida 

Department 

of Health 

State of New 

Departm

Environm

Protec

Lab ID 

11674 

NELAP 

Primary 

Lab ID 

01138CA 

NELAP 

Secondary 

Lab ID E871007 

NELAP Primary 

Lab ID CA

NELAP Se

TABLE 1 

NP

W S NPW S Dr. W NP W S T Dr. W NP W 

Acenaphthene 9 20 9 20Phenanthrene 9 20 9 20Fluorene 9 20 9 20Naphthalene 9 20 9 20

www.axysanalytical.com
AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 

Table 1b 

NELAP Accreditation Held by AXYS Analytical Services Ltd. 

for Perfluorinated Organic Compounds

Matrix Codes for Table 1b NPW = Non-Potable Water DrW = Drinking WaterS = Solid T = Tissue Accreditation Method Codes and Explanation for Table 1b Code Accreditation Certificate Applicable AXYS Method and Description

No. Method Reference

12 AXYS MLA-041 MLA-041, laboratory performance based method (LC/MS-MS) 13 AXYS MLA-043 MLA-043, laboratory performance based method (LC/MS-MS) 14 AXYS MLA-060 MLA-060, laboratory performance based method (LC/MS-MS) 

State of Florida 

Department 

of Health

Minnesota Department 

of Health

State of New 

Departm

Environm

Protec

Lab ID E871007 

NELAP Primary

Lab ID 232-999-430 

NELAP Primary

Lab ID CA

NELAP Se

TABLE 1 

 Dr. 

W

NP 

W S T Dr. W NP W S T Dr. W NP W 

PFC – Perfluorinated Organic Compounds Perfluorobutanoate (PFBA) Note 14 14 12 13 14 14 12 13 14 14 Perfluoropentanoate (PFPeA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorohexanoate (PFHxA) Note 14 14 12 13 14 14 12 13 14 14 Perfluoroheptanoate (PFHpA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorooctanoate (PFOA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorononanoate (PFNA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorodecanoate (PFDA) Note 14 14 12 13 14 14 12 13 14 14 Perfluoroundecanoate (PFUnA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorododecanoate (PFDoA) Note 14 14 12 13 14 14 12 13 14 14 Perfluorobutanesulfonate (PFBS) 14 14 12 13 14 14 12 13 14 14 Perfluorohexanesulfonate (PFHxS) 14 14 12 13 14 14 12 13 14 14 Perfluorooctanesulfonate (PFOS) 14 14 12 13 14 14 12 13 14 14 Perfluorooctane sulfonamide (PFOSA) 14 14 12 13 14 14 12 13 Note: Accreditations by Minnesota Department of Health and New Jersey Department of Environmental Protection are against the corresponding 

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AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 Page 7 of 18 

Table 2: 

Canadian and US State Specific Accreditation Held by AXYS Analytical Services Ltd. 

Matrix Codes for Table 2 

NP W = Non-Potable Water 

Dr. W = Drinking Water 

W = Aqueous 

S = Solid 

T = Tissue 

Accreditation Method Codes and Explanation for Table 2 

Code Accreditation Certificate Applicable AXYS Method and Description

No. Method Reference

1 EPA 1613 MLA-017 Performance based implementation of EPA 1613B (GC/HRMS) 

3 AXYS MLA-017 MLA-017 Performance based implementation of EPA 1613B (GC/HRMS) 

7 EPA 1668A MLA-010 Performance based implementation of EPA 1668A (GC/HRMS) 

10 AXYS MLA-007 MLA-007, Performance based modification of EPA 8270C/D, 8081A/B 

 (GC/LRMS and GC/ECD) 

12 AXYS MLA-041 MLA-041 Laboratory performance based method (LC/MS-MS) 

13 AXYS MLA-043 MLA-043 Laboratory performance based method (LC/MS-MS) 

14 AXYS MLA-060 MLA-060 Laboratory performance based method (LC/MS-MS) 

15 AXYS MLA-010 MLA-010 Performance based implementation of EPA 1668A (GC/HRMS) 

16 AXYS MLA-028 MLA-028 Laboratory performance based method (GC/HRMS) 

17 AXYS MLA-033 MLA-033 Performance based implementation of EPA 1614 (GC/HRMS) 

18 AXYS MLA-021 MLA-021 Performance based modification of EPA 8270C/D (GC/LRMS) 

19 AXYS MLA-075 MLA-075 Performance based implementation of EPA 1694 (LC/MS-MS) 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

PCDD/F - Polychlorinated Dioxins and Furans 

1,2,3,4,6,7,8-HpCDD 3 3 3 3 1 1 

1,2,3,4,6,7,8-HpCDF 3 3 3 3 1 1 

1,2,3,4,7,8,9-HpCDF 3 3 3 3 1 1 

1,2,3,4,7,8-HxCDD 3 3 3 3 1 1 

1,2,3,4,7,8-HxCDF 3 3 3 3 1 1 

1,2,3,6,7,8-HxCDD 3 3 3 3 1 1 

1,2,3,6,7,8-HxCDF 3 3 3 3 1 1 

1,2,3,7,8,9-HxCDD 3 3 3 3 1 1 

1,2,3,7,8,9-HxCDF 3 3 3 3 1 1 

1,2,3,7,8-PeCDD 3 3 3 3 1 1 

1,2,3,7,8-PeCDF 3 3 3 3 1 1 

2,3,4,6,7,8-HxCDF 3 3 3 3 1 1 

2,3,4,7,8-PeCDF 3 3 3 3 1 1 

2,3,7,8-TCDD 3 3 3 3 1 1 

2,3,7,8-TCDF 3 3 3 3 1 1 

OCDD 3 3 3 3 1 1 

OCDF 3 3 3 3 1 1 

Total TCDD 1 1 

Total TCDF 1 1 

Total PeCDD 1 1 

Total PeCDF 1 1 

Total HxCDD 1 1 

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Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

Total HxCDF 1 1 

Total HpCDD 1 1 

Total HpCDF 1 1 

Total PCDD 1 1 

Total PCDF 1 1 

Total PCDD + PCDF 1 1 

 

PCBs – Polychlorinated biphenyls 

PCB 1 2-Chlorobiphenyl 15 15 15 7 7 

PCB 2 3-Chlorobiphenyl 15 15 15 7 7 

PCB 3 4-Chlorobiphenyl 15 15 15 7 7 

PCB 4 2,2'-Dichlorobiphenyl 15 15 15 7 7 

PCB 5 2,3-Dichlorobiphenyl 15 15 15 7 7 

PCB 6 2,3'-Dichlorobiphenyl 15 15 15 7 7 

PCB 7 2,4-Dichlorobiphenyl 15 15 15 7 7 

PCB 8 2,4'-Dichlorobiphenyl 15 15 15 7 7 

PCB 8/5 10 10 10 

PCB 9 2,5-Dichlorobiphenyl 15 15 15 7 7 

PCB 10 2,6-Dichlorobiphenyl 15 15 15 7 7 

PCB 11 3,3'-Dichlorobiphenyl 15 15 15 7 7 

PCB 12 3,4-Dichlorobiphenyl 15 15 15 7 7 

PCB 13 3,4'-Dichlorobiphenyl 15 15 15 7 7 

PCB 14 3,5-Dichlorobiphenyl 15 15 15 7 7 

PCB 15 4,4'-Dichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 16 2,2',3-Trichlorobiphenyl 15 15 15 7 7 

PCB 16/32 10 10 10 

PCB 17 2,2',4-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 18 2,2',5-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 19 2,2',6-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 20 2,3,3'-Trichlorobiphenyl 15 15 15 7 7 

PCB 21 2,3,4-Trichlorobiphenyl 15 15 15 7 7 

PCB 22 2,3,4'-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 23 2,3,5-Trichlorobiphenyl 15 15 15 7 7 

PCB 24 2,3,6-Trichlorobiphenyl 15 15 15 7 7 

PCB 24/27 10 10 10 

PCB 25 2,3',4-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 26 2,3',5-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 27 2,3',6-Trichlorobiphenyl 15 15 15 7 7 

PCB 28 2,4,4'-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 29 2,4,5-Trichlorobiphenyl 15 15 15 7 7 

PCB 30 2,4,6-Trichlorobiphenyl 15 15 15 7 7 

PCB 31 2,4',5-Trichlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 32 2,4',6-Trichlorobiphenyl 15 15 15 7 7 

PCB 33 2,3',4'-Trichlorobiphenyl 15 15 15 7 7 

PCB 33/20/21 18 10 10 

PCB 34 2,3',5'-Trichlorobiphenyl 15 15 15 7 7 

PCB 35 3,3',4-Trichlorobiphenyl 15 15 15 7 7 

PCB 36 3,3',5-Trichlorobiphenyl 15 15 15 7 7 

PCB 37 3,4,4'-Trichlorobiphenyl 15 15 15 7 7 

PCB 38 3,4,5-Trichlorobiphenyl 15 15 15 7 7 

PCB 39 3,4',5-Trichlorobiphenyl 15 15 15 7 7 

PCB 40 2,2',3,3'-Tetrachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 41 2,2',3,4-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 41/71/64/68 10 10 10 

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Canadian Association for 

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(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

PCB 42 2,2',3,4'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 42/59 10 10 10 

PCB 43 2,2',3,5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 44 2,2',3,5'-Tetrachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 45 2,2',3,6-Tetrachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 46 2,2',3,6'-Tetrachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 47 2,2',4,4'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 47/48/75 10 10 10 

PCB 48 2,2',4,5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 49 2,2',4,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 49/43 10 10 10 

PCB 50 2,2',4,6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 51 2,2',4,6'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 52 2,2',5,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 52/73 10 10 10 

PCB 53 2,2',5,6'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 54 2,2',6,6'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 55 2,3,3',4-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 56 2,3,3',4'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 56/60 10 10 10 

PCB 57 2,3,3',5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 58 2,3,3',5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 59 2,3,3',6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 60 2,3,4,4'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 61 2,3,4,5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 62 2,3,4,6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 63 2,3,4',5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 64 2,3,4',6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 65 2,3,5,6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 66 2,3',4,4'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 66/80 10 10 10 

PCB 67 2,3',4,5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 68 2,3',4,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 69 2,3',4,6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 70 2,3',4',5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 70/76 10 10 10 

PCB 71 2,3',4',6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 72 2,3',5,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 73 2,3',5',6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 74 2,4,4',5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 74/61 10 10 10 

PCB 75 2,4,4',6-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 76 2,3',4',5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 77 3,3',4,4'-Tetrachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 78 3,3',4,5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 79 3,3',4,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 80 3,3',5,5'-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 81 3,4,4',5-Tetrachlorobiphenyl 15 15 15 7 7 

PCB 82 2,2',3,3',4-Pentachlorobiphenyl 15 15 15 7 7 

PCB 83 2,2',3,3',5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 83/108 10 10 10 

PCB 84 2,2',3,3',6-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 85 2,2',3,4,4'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 85/120 10 10 10 

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Canadian Association for 

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(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

PCB 86 2,2',3,4,5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 87 2,2',3,4,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 87/115/116 10 10 10 

PCB 88 2,2',3,4,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 89 2,2',3,4,6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 90 2,2',3,4',5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 91 2,2',3,4',6-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 92 2,2',3,5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 93 2,2',3,5,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 94 2,2',3,5,6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 95 2,2',3,5',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 95/93 10 10 10 

PCB 96 2,2',3,6,6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 97 2,2',3,4',5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 97/86 10 10 10 

PCB 98 2,2',3,4',6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 99 2,2',4,4',5-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 100 2,2',4,4',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 101 2,2',4,5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 101/90/89 10 10 10 

PCB 102 2,2',4,5,6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 103 2,2',4,5',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 104 2,2',4,6,6'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 105 2,3,3',4,4'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 105/127 10 10 10 

PCB 106 2,3,3',4,5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 107 2,3,3',4',5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 107/109 10 10 10 

PCB 108 2,3,3',4,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 109 2,3,3',4,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 110 2,3,3',4',6-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 111 2,3,3',5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 112 2,3,3',5,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 113 2,3,3',5',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 114 2,3,4,4',5-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 115 2,3,4,4',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 116 2,3,4,5,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 117 2,3,4',5,6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 118 2,3',4,4',5-Pentachlorobiphenyl 15 15 15 7 7 

PCB 118/116 10 10 10 

PCB 119 2,3',4,4',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 120 2,3',4,5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 121 2,3',4,5',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 122 2,3,3',4',5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 123 2,3',4,4',5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 124 2,3',4',5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 125 2,3',4',5',6-Pentachlorobiphenyl 15 15 15 7 7 

PCB 126 3,3',4,4',5-Pentachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 127 3,3',4,5,5'-Pentachlorobiphenyl 15 15 15 7 7 

PCB 128 2,2',3,3',4,4'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 129 2,2',3,3',4,5-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 130 2,2',3,3',4,5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 131 2,2',3,3',4,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 131/142 10 10 10 

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ACC-101 Rev 06, 29-Jun-2011 Page 11 of 18 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

PCB 132 2,2',3,3',4,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 133 2,2',3,3',5,5'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 134 2,2',3,3',5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 134/143 10 10 10 

PCB 135 2,2',3,3',5,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 136 2,2',3,3',6,6'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 137 2,2',3,4,4',5-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 138 2,2',3,4,4',5'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 138/163/164 10 10 10 

PCB 139 2,2',3,4,4',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 140 2,2',3,4,4',6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 141 2,2',3,4,5,5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 142 2,2',3,4,5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 143 2,2',3,4,5,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 144 2,2',3,4,5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 144/135 10 10 10 

PCB 145 2,2',3,4,6,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 146 2,2',3,4',5,5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 147 2,2',3,4',5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 148 2,2',3,4',5,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 149 2,2',3,4',5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 149/139 10 10 10 

PCB 150 2,2',3,4',6,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 151 2,2',3,5,5',6-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 152 2,2',3,5,6,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 153 2,2',4,4',5,5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 154 2,2',4,4',5,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 155 2,2',4,4',6,6'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 156 2,3,3',4,4',5-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 157 2,3,3',4,4',5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 158 2,3,3',4,4',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 158/160 10 10 10 

PCB 159 2,3,3',4,5,5'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 160 2,3,3',4,5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 161 2,3,3',4,5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 162 2,3,3',4',5,5'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 163 2,3,3',4',5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 164 2,3,3',4',5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 165 2,3,3',5,5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 166 2,3,4,4',5,6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 167 2,3',4,4',5,5'-Hexachlorobiphenyl 15 15 15 7 7 

PCB 168 2,3',4,4',5',6-Hexachlorobiphenyl 15 15 15 7 7 

PCB 169 3,3',4,4',5,5'-Hexachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 170 2,2',3,3',4,4',5-Heptachlorobiphenyl 15 15 15 7 7 

PCB 170/190 10 10 10 

PCB 171 2,2',3,3',4,4',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 172 2,2',3,3',4,5,5'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 172/192 10 10 10 

PCB 173 2,2',3,3',4,5,6-Heptachlorobiphenyl 15 15 15 7 7 

PCB 174 2,2',3,3',4,5,6'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 174/181 10 10 10 

PCB 175 2,2',3,3',4,5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 176 2,2',3,3',4,6,6'-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 177 2,2',3,3',4,5',6'-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

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AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 Page 12 of 18 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

PCB 178 2,2',3,3',5,5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 179 2,2',3,3',5,6,6'-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 180 2,2',3,4,4',5,5'-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 181 2,2',3,4,4',5,6-Heptachlorobiphenyl 15 15 15 7 7 

PCB 182 2,2',3,4,4',5,6'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 183 2,2',3,4,4',5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 184 2,2',3,4,4',6,6'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 185 2,2',3,4,5,5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 186 2,2',3,4,5,6,6'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 187 2,2',3,4',5,5',6-Heptachlorobiphenyl 15 15 15 7 7 

PCB 187/182 10 10 10 

PCB 188 2,2',3,4',5,6,6'-Heptachlorobiphenyl 15 15 15 7 7 

PCB 189 2,3,3',4,4',5,5'-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 190 2,3,3',4,4',5,6-Heptachlorobiphenyl 15 15 15 7 7 

PCB 191 2,3,3',4,4',5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 192 2,3,3',4,5,5',6-Heptachlorobiphenyl 15 15 15 7 7 

PCB 193 2,3,3',4',5,5',6-Heptachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 194 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 195 2,2',3,3',4,4',5,6-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 196 2,2',3,3',4,4',5,6'-Octachlorobiphenyl 15 15 15 7 7 

PCB 196/203 10 10 10 

PCB 197 2,2',3,3',4,4',6,6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 198 2,2',3,3',4,5,5',6-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 199 2,2',3,3',4,5,5',6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 200 2,2',3,3',4,5,6,6'-Octachlorobiphenyl 15 15 15 7 7 

PCB 201 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 202 2,2',3,3',5,5',6,6'-Octachlorobiphenyl 15 15 15 7 7 

PCB 203 2,2',3,4,4',5,5',6-Octachlorobiphenyl 15 15 15 7 7 

PCB 204 2,2',3,4,4',5,6,6'-Octachlorobiphenyl 15 15 15 7 7 

PCB 205 2,3,3',4,4',5,5',6-Octachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 206 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 207 2,2',3,3',4,4',5,6,6'-Nonachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 208 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

PCB 209 Decachlorobiphenyl 10, 15 10, 15 10, 15 7 7 

Total Monochlorobiphenyls 15 15 15 

Total Dichlorobiphenyls 10, 15 10, 15 10, 15 

Total Trichlorobiphenyls 10, 15 10, 15 10, 15 

Total Tetrachlorobiphenyls 10, 15 10, 15 10, 15 

Total Pentachlorobiphenyls 10, 15 10, 15 10, 15 

Total Hexachlorobiphenyls 10, 15 10, 15 10, 15 

Total Heptachlorobiphenyls 10, 15 10, 15 10, 15 

Total Octachlorobiphenyls 10, 15 10, 15 10, 15 

Total Nonachlorobiphenyls 10, 15 10, 15 10, 15 

Total Decachlorobiphenyls 10 10 10 

Total Polychlorinated biphenyls 10 10 10 7 

 

Aroclors 

Aroclor 1260 10 10 10 7 7 

Aroclor 1254 10 10 10 7 7 

Aroclor 1268 10 10 10 

Aroclor 1221 10 10 10 7 7 

Aroclor 1232 10 10 10 7 7 

Aroclor 1248 10 10 10 7 7 

Aroclor 1016 7 7 

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ACC-101 Rev 06, 29-Jun-2011 Page 13 of 18 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

Aroclor 1242 7 7 

Aroclor 1242/1016 10 10 10 

 

Pesticides 

2,4'-DDD 10, 16 10, 16 10, 16 16 

2,4'-DDE 10, 16 10, 16 10, 16 16 

2,4'-DDT 10, 16 10, 16 10, 16 16 

4,4'-DDD 10, 16 10, 16 10, 16 16 

4,4'-DDE 10, 16 10, 16 10, 16 16 

4,4'-DDT 10, 16 10, 16 10, 16 16 

Aldrin 10, 16 10, 16 10, 16 16 

Alpha-HCH 10, 16 10, 16 10, 16 16 

Beta-HCH 10, 16 10, 16 10, 16 16 

cis-Chlordane (alpha-Chlordane) 10, 16 10, 16 10, 16 16 

cis-Nonachlor 10, 16 10, 16 10, 16 16 

Delta-HCH 10, 16 10, 16 10, 16 16 

Dieldrin 10, 16 10, 16 10, 16 16 

Endosulphan I 10, 16 10, 16 10, 16 16 

Endosulphan II 10, 16 10, 16 10, 16 16 

Endosulphan sulphate 10, 16 10, 16 10, 16 16 

Endrin 10, 16 10, 16 10, 16 16 

Endrin aldehyde 10, 16 10, 16 16 16 

Endrin ketone 10, 16 10, 16 10, 16 16 

Gamma-HCH (Lindane) 10, 16 10, 16 10, 16 16 

Heptachlor 10, 16 10, 16 10, 16 16 

Heptachlor epoxide 10, 16 10, 16 10, 16 16 

Hexachlorobenzene 10, 16 10, 16 10, 16 16 

Hexachlorobutadiene 16 16 

Methoxychlor 10, 16 10, 16 10, 16 16 

Mirex 10, 16 10, 16 10, 16 16 

Oxychlordane 10, 16 10, 16 10, 16 16 

Toxaphene 10 10 10 

trans-Chlordane (gamma-Chlordane) 10, 16 10, 16 10, 16 16 

trans-Nonachlor 16 10, 16 10, 16 16 

 

BDE - Brominated Diphenylethers 

BDE 7 2,4-dibromodiphenylether 17 17 17 

BDE 8 2,4’-dibromodiphenylether 17 17 17 

BDE 10 2,6-dibromodiphenylether 17 17 17 

BDE 11 3,3’-dibromodiphenylether 17 17 17 

BDE 12 3,4-dibromodiphenylether 17 17 17 

BDE 13 3,4’-dibromodiphenylether 17 17 17 

BDE 15 4,4’-dibromodiphenylether 17 17 17 

BDE 17 2,2’,4-tribromodiphenylether 17 17 17 

BDE 25 2,3’,4-tribromodiphenylether 17 17 17 

BDE 28 2,4,4’-tribromodiphenylether 17 17 17 

BDE 30 2,4,6-tribromodiphenylether 17 17 17 

BDE-33 2’,3,4-tribromodiphenylether 17 17 17 

BDE 35 3,3’,4-tribromodiphenylether 17 17 17 

BDE 37 3,4,4’-tribromodiphenylether 17 17 17 

BDE 47 2,2’,4,4’-tetrabromodiphenylether 17 17 17 

BDE 49 2,2’,4,5’-tetrabromodiphenylether 17 17 17 

BDE 66 2,3’,4,4’-tetrabromodiphenylether 17 17 17 

BDE 75 2,4,4’,6-tetrabromodiphenylether 17 17 17 

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ACC-101 Rev 06, 29-Jun-2011 Page 14 of 18 

Canadian Association for 

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(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

BDE 77 3,3’,4,4’-tetrabromodiphenylether 17 17 17 

BDE 85 2,2’,3,4,4’-pentabromodiphenylether 17 17 17 

BDE 99 2,2’,4,4’,5-pentabromodiphenylether 17 17 17 

BDE 100 2,2’,4,4’,6-pentabromodiphenylether 17 17 17 

BDE 105 2,3,3’,4,4’-pentabromodiphenylether 17 17 17 

BDE 116 2,3,4,5,6-pentabromodiphenylether 17 17 17 

BDE 119 2,3’,4,4’,6-pentabromodiphenylether 17 17 17 

BDE 126 3,3’,4,4’,5-pentabromodiphenylether 17 17 17 

BDE 140 2,2’,3,4,4’,6’-hexabromodiphenylether 17 17 17 

BDE 153 2,2’,4,4’,5,5’-hexabromodiphenylether 17 17 17 

BDE 154 2,2’,4,4’,5’,6-hexabromodiphenylether 17 17 17 

BDE 155 2,2’,4,4’,6,6’-hexabromodiphenylether 17 17 17 

BDE 166 2,3,4,4’,5,6-hexabromodiphenylether 17 17 17 

BDE 181 2,2’,3,4,4’,5,6-heptabromodiphenylether 17 17 17 

BDE-183 2,2’,3,4,4’,5’,6-heptabromodiphenylether 17 17 17 

BDE 190 2,3,3’,4,4’,5,6-heptabromodiphenylether 17 17 17 

BDE 206 2,2’,3,3’,4,4’,5,5’,6-nonabromodiphenylether 17 17 17 

BDE 207 2,2’,3,3’,4,4’,5,6,6’-nonabromodiphenylether 17 17 17 

BDE 208 2,2’,3,3’,4,5,5’,6,6’-nonabromodiphenylether 17 17 17 

BDE 209 Decabromodiphenylether 17 17 17 

 

PFC – Perfluorinated Organic Compounds 

Perfluorobutanoate (PFBA) 14 12 13 

Perfluoropentanoate (PFPeA) 14 12 13 

Perfluorohexanoate (PFHxA) 14 12 13 

Perfluoroheptanoate (PFHpA) 14 12 13 

Perfluorooctanoate (PFOA) 14 12 13 

Perfluorononanoate (PFNA) 14 12 13 

Perfluorodecanoate (PFDA) 14 12 13 

Perfluoroundecanoate (PFUnA) 14 12 13 

Perfluorododecanoate (PFDoA) 14 12 13 

Perfluorobutanesulfonate (PFBS) 14 12 13 

Perfluorohexanesulfonate (PFHxS) 14 12 13 

Perfluorooctanesulfonate (PFOS) 14 12 13 

Perfluorooctane sulfonamide (PFOSA) 14 12 13 

 

PAH 

Anthracene 18 18 

Pyrene 18 18 

Benzo[ghi]perylene 18 18 

Benzo[e]pyrene 18 18 

Indeno[1,2,3-cd]pyrene 18 18 

Perylene 18 18 

Benzo[b]fluoranthene 18 18 

Fluoranthene 18 18 

Benzo[k]fluoranthene 18 

Acenaphthylene 18 18 

Chrysene 18 18 

Benzo[a]pyrene 18 18 

Dibenz[ah]anthracene 18 18 

Benz[a]anthracene 18 18 

Acenaphthene 18 18 

Phenanthrene 18 18 

Fluorene 18 18 

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AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 Page 15 of 18 

Canadian Association for 

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(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

Naphthalene 18 18 

 

PPCP (Pharmaceutical and Personal Care Products) 

Acetaminophen 19 19 

Azithromycin 19 19 

Caffeine 19 19 

Carbadox 19 19 

Carbamazepine 19 19 

Cefotaxime 19 19 

Ciprofloxacin 19 19 

Clarithromycin 19 19 

Clinafloxacin 19 19 

Cloxacillin 19 19 

Dehydronifedipine 19 19 

Digoxigenin 19 19 

Digoxin 19 19 

Diltiazem 19 19 

1,7-Dimethylxanthine 19 19 

Diphenhydramine 19 19 

Enrofloxacin 19 19 

Erythromycin 19 19 

Flumequine 19 19 

Fluoxetine 19 19 

Lincomycin 19 19 

Lomefloxacin 19 19 

Miconazole 19 19 

Norfloxacin 19 19 

Norgestimate 19 19 

Ofloxacin 19 19 

Ormetoprim 19 19 

Oxacillin 19 19 

Oxolinic acid 19 19 

Penicillin G 19 19 

Penicillin V 19 19 

Roxithromycin 19 19 

Sarafloxacin 19 19 

Sulfachloropyridazine 19 19 

Sulfadiazine 19 19 

Sulfadimethoxine 19 19 

Sulfamerazine 19 19 

Sulfamethazine 19 19 

Sulfamethizole 19 19 

Sulfamethoxazole 19 19 

Sulfanilamide 19 19 

Sulfathiazole 19 19 

Thiabendazole 19 19 

Trimethoprim 19 19 

Tylosin 19 19 

Virginiamycin 19 19 

 

Anhydrochlortetracycline (ACTC) 19 19 

Anhydrotetracycline (ATC) 19 19 

Chlortetracycline (CTC) 19 19 

Demeclocycline 19 19 

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AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 Page 16 of 18 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

Doxycycline 19 19 

4-Epianhydrochlortetracycline (EACTC) 19 19 

4-Epianhydrotetracycline (EATC) 19 19 

4-Epichlortetracycline (ECTC) 19 19 

4-Epioxytetracycline (EOTC) 19 19 

4-Epitetracycline (ETC) 19 19 

Isochlortetracycline (ICTC) 19 19 

Minocycline 19 19 

Oxytetracycline (OTC) 19 19 

Tetracycline (TC) 19 19 

 

Bisphenol A 19 19 

Furosemide 19 19 

Gemfibrozil 19 19 

Glipizide 19 19 

Glyburide 19 19 

Hydrochlorothiazide 19 19 

2-hydroxy-ibuprofen 19 19 

Ibuprofen 19 19 

Naproxen 19 19 

Triclocarban 19 19 

Triclosan 19 19 

Warfarin 19 19 

 

Albuterol 19 19 

Amphetamine 19 19 

Atenolol 19 19 

Atorvastatin 19 19 

Cimetidine 19 19 

Clonidine 19 19 

Codeine 19 19 

Cotinine 19 19 

Enalapril 19 19 

Hydrocodone 19 19 

Metformin 19 19 

Oxycodone 19 19 

Ranitidine 19 19 

Triamterene 19 19 

 

Alprazolam 19 19 

Amitriptyline 19 19 

Amlodipine 19 19 

Benzoylecgonine 19 19 

Benztropine 19 19 

Betamethasone 19 19 

Cocaine 19 19 

DEET (N,N-diethyl-m-toluamide) 19 19 

Desmethyldiltiazem 19 19 

Diazepam 19 19 

Fluocinonide 19 19 

Fluticasone propionate 19 19 

Hydrocortisone 19 19 

10-hydroxy-amitriptyline 19 19 

Meprobamate 19 19 

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AXYS Analytical Services Ltd. 

ACC-101 Rev 06, 29-Jun-2011 Page 17 of 18 

Canadian Association for 

Laboratory Accreditation 

(CALA)

Washington 

State 

Department 

of Ecology

Accreditation No.: 

A 2637 Lab. ID: C404

TABLE 2

W S Pulp T NP 

W S

Methylprednisolone 19 19 

Metoprolol 19 19 

Norfluoxetine 19 19 

Norverapamil 19 19 

Paroxetine 19 19 

Prednisolone 19 19 

Prednisone 19 19 

Promethazine 19 19 

Propoxyphene 19 19 

Propranolol 19 19 

Sertraline 19 19 

Simvastatin 19 19 

Theophylline 19 19 

Trenbolone 19 19 

Trenbolone acetate 19 19 

Valsartan 19 19 

Verapamil 19 19 

Table 1 and Table 2 - Explanation of Terms Used: 

• NELAP = National Environmental Laboratory Accreditation Program 

• Non-potable water = water not fit for consumption without treatment as it may contain 

pollutants, contaminants, minerals or infective agents. Surface water, ground water, rainwater, 

effluents as well as any other non-drinking water sources are included in this category. 

• Solid = environmental solid sample. Soil, sediment, biosolids, hazardous waste, mixed phase 

samples with significant solids content are included in this category. 

• Performance based implementation = methodology follows that of the method reference but 

modifications deemed by AXYS as minor 1 may apply, results meet method reference data 

quality standard. 

• Performance based modification = modifications deemed by AXYS as significant2

 have been 

made to method reference protocol, results meet method reference accuracy standard. The 

suitability of the methodology for any method prescriptive applications should be assessed 

based on the modifications made and the specific work requirements. 

• Performance based method = an in-house AXYS method, published method reference not 

applicable. 

• GC/LRMS = gas chromatography, low resolution mass spectrometry detection. 

• GC/HRMS = gas chromatography, high resolution mass spectrometry detection. 

• GC/ECD = gas chromatography, electron capture detection. 

• LC/MS-MS = liquid chromatography, mass spectrometry-mass spectrometry detection. 

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ACC-101 Rev 06, 29-Jun-2011 Page 18 of 18 

Note 1: 

Performance Based Implementation - Examples of Minor Modifications 

- use of additional isotopically labeled references 

- adjustment of calibration range 

- adjustment of clean-up technique 

- use of a different extraction of same general type (example soxhlet vs soxhlet Dean Stark) 

- addition of matrix type using same principles (example addition of tissue matrix using same 

detection principle and similar extraction type) 

Note 2: 

Performance Based Modification - Examples of Significant Modifications 

- different acquisition conditions using same detection principle (example MS SIM vs. full scan) 

- different internal control limits while meeting method reference accuracy standard 

www.axysanalytical.com Page 102 of 102