# Patent

## Title
Synthesis of free radical polymerizable ethylenically unsaturated poloxamers and poloxamines

## Abstract

A new synthetic method of forming free radical polymerizable end terminal functionalized poloxamers and poloxamines in commercial scale batches which uses a binary system of inhibitors to eliminate the formation of high molecular weight impurities. It has also been surprisingly discovered that the inorganic impurities formed during the commercial scale synthetic process are removed by the use of resin materials.

## Background
BACKGROUND OF THE INVENTION
Poloxamer F127 block copolymers are known compounds and are generally available under the trademark PLURONIC. These block copolymers consist of hydrophilic poly(ethylene oxide) (PEO) and hydrophobic poly(propylene oxide) (PPO) blocks arranged in A-B-A tri-block structure: PEO-PPO-PEO with end terminal hydroxyl groups. In some instances, unmodified Poloxamer can be used in medical devices such as ophthalmic lenses.
Poloxamers have the following general formula:

HO(C2H4O)a(C3H6O)b(C2H4O)aH

Reverse poloxamers are also known block copolymers and have the following general formula:

HO(C3H6O)b(C2H4O)a(C3H6O)bH

wherein a and b may be the same or different and are of varying lengths.

Poloxamers and reverse poloxamers have end terminal hydroxyl groups that can be functionalized to be free radical polymerizable ethylenically unsaturated end groups. An example of a free radical polymerizable ethylenically unsaturated end terminal functionalized poloxamer is poloxamer dimethacrylate (e.g., PLURONIC F-127 dimethacrylate) as disclosed in US Patent Publication No. 2003/0044468 to Cellesi et al. U.S. Pat. No. 6,517,933 discloses glycidyl-terminated copolymers of polyethylene glycol and polypropylene glycol. U.S. Pat. No. 8,377,464 discloses polymerizable surfactants and a method of making same.
Poloxamers and reverse poloxamers are surfactants with varying HLB values based upon the varying values of a and b, a representing the number of hydrophilic poly(ethylene oxide) units (PEO) being present in the molecule and b representing the number of hydrophobic poly(propylene oxide) units (PPO) being present in the molecule. While poloxamers and reverse poloxamers are considered to be difunctional molecules (based on the terminal hydroxyl groups) they are also available in a tetrafunctional form known as poloxamines, trade name TETRONIC. For poloxamines, the molecules are tetrafunctional block copolymers terminating in primary hydroxyl groups and linked by a central diamine. Poloxamines have the following general structure:




Reverse poloxamines are also known and have varying HLB values based upon the relative ratios of a to b wherein a and b are as defined above.

Polyethers, such as poloxamers and poloxamines block copolymers, that are present at the surface of substrates have long been known to inhibit bacterial adhesion and to reduce the amount of lipid and protein deposition (non-fouling surface). In the present invention, we provide an improved synthesis of free radical polymerizable ethylenically unsaturated end terminal functionalized poloxamer and/or poloxamine block copolymers (BASF Corp.). The improved synthesis of free radical polymerizable ethylenically unsaturated end terminal functionalized poloxamer and/or poloxamine block copolymers provides improved overall device performance when the block copolymers prepared according to the invention described herein are included in ophthalmic device forming formulations.
It is known the terminal hydroxyl groups of PLURONIC F-127 can be functionalized to provide the desired reactivity/purpose as part of the total monomer mix, before/after polymerization. Most of the materials described in the literature that respond to external stimuli are acrylic group containing molecules. So, for example, acrylated PLURONIC F-127 is of importance in some lens formulation.
Whether to acrylate the terminal hydroxyl groups in poloxamers can be determined by the functional group of the other reactive molecules in the monomer mixture. The acrylation can be achieved by the reaction of (unmodified) poloxamers and poloxamines such as PLURONIC F-127 with methacryloyl chloride or methacryloyl anhydride in the presence of a suitable base. Since PLURONIC F-127 is an EO-PO block copolymer, it is known to be susceptible to possible degradation upon coming into contact with free radicals; thus yielding undesired reaction byproducts such as high molecular weight impurities when produced under commercial manufacturing conditions. In addition, residual reaction solvent(s), inhibitors, inorganic salts and methacrylic acid (MAA) may also remain in the free radical polymerizable ethylenically unsaturated end terminal functionalized poloxamers and reverse poloxamers. An example of a free radical polymerizable ethylenically unsaturated end terminal functionalized poloxamer is poloxamer dimethacrylate (e.g., PLURONIC F-127 dimethacrylate). PLURONIC F-127 DM, (DM meaning dimethacrylate), made by previously known commercial scale synthetic methods resulted in a grade of material which was routinely outside of the manufacturing specifications for use in forming certain medical devices. This is important because these byproducts may be injurious to the desired polymerization of PLURONIC F-127 DM, particularly when they are being used in a monomer mix for contact lens production. Any undesired and/or high molecular weight impurity can greatly interfere with lens processing, as well as properties and/or performance of the commercialized contact lens. Therefore, it is desirable that functionalized block copolymers such as PLURONIC F-127 DM prepared in commercial scale amounts be free of any high molecular weight impurities which are beyond the limits set in the manufacturing specifications.
In the commercial scale synthesis of PLURONIC F-127 DM, 2,6-ditert-butyl-4-methyl phenol (BHT) is added as a free radical inhibitor to minimize the formation of these undesired byproducts. However, this synthesis is still unable to prevent the formation of high molecular weight impurities and inorganic salts. These impurities pose a manufacturing risk during the filtration of a monomer mix for making hydrophilic contact lenses containing same by slowing down the filtering process considerably; and, as a result, premature curing, i.e., polymerization, of the monomer mix commences before the monomer mix is introduced to the mold.
Premature curing (polymerization) of monomer materials is undesirable as it renders the monomer mix unsuitable for preparing the desired final product, e.g. contact lenses, and can cause clogging of machines used to make such products. In particular, when the monomer mix starts to polymerize prematurely it slowly forms a gel, typically from the bottom of the vessel upwards. Some monomer mixtures, however, auto-accelerate and can react quite violently.
Therefore, there is still a need for an improved commercial scale synthetic process for providing a high purity functionalized block copolymers, such as PLURONIC F-127 DM.
SUMMARY OF THE INVENTION
This invention describes a new synthetic method of forming free radical polymerizable end terminal functionalized poloxamers and poloxamines in commercial scale batches which uses a binary system of inhibitors to eliminate the formation of high molecular weight impurities. It has also been surprisingly discovered that the inorganic impurities formed during the commercial scale synthetic process are removed by the use of resin materials.
To minimize the high molecular weight impurities previously formed during the commercial scale synthesis of poloxamer 407 DM and to develop different binary systems of inhibitors to avoid these high molecular impurities during the synthesis of poloxamer 407 DM, a novel commercial scale synthetic process is disclosed herein.
Disclosed herein is a method comprising reacting a poloxamer or poloxamine compound with a first methacrylating agent in the presence of a solvent stabilized with a first free radical inhibitor to form a first reaction product, and further reacting the first reaction product with an organic base and a second methacrylating agent stabilized with a second free radical inhibitor to form a second reaction product, wherein the first free radical inhibitor is a different compound than the second free radical inhibitor.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 is the NMR Spectrum of the product of Example 1;
FIG. 2 to FIG. 9 are HPLC chromatograms showing the calculations of the product of Example 1;
FIG. 10 to FIG. 13 are GC chromatograms showing the calculations of the product of Example 1;
FIG. 14 is the HPLC chromatogram showing the high molecular weight component identification of the product of Example 1;
FIG. 15 is the GPC chromatogram of the product of Example 1;
FIG. 16 is the NMR Spectrum of the product of Comparative Example 1 when the reaction is performed without using any inhibitor;
FIG. 17 to FIG. 24 are HPLC chromatograms showing the calculations of the product of Comparative Example 1 when the reaction is performed without using any inhibitor;
FIG. 25 to FIG. 29 are GC chromatograms showing the calculations of the product of Comparative Example 1 when the reaction is performed without using any inhibitor;
FIG. 30 is the HPLC chromatogram showing the high molecular weight component identification of the product of Comparative Example 1 when the reaction is performed without using any inhibitor;
FIG. 31 is the GPC chromatogram of the product of Comparative Example 1 when the reaction is performed without using any inhibitor;
FIG. 32 is the NMR Spectrum of the batch FS-09903-070 when the reaction is performed using BHT and MEHQ as inhibitors for the optimized improved large scale synthesis of Poloxamer 407DM of Example 3;
FIG. 33 to FIG. 39 are HPLC chromatograms showing the calculations of the batch FS-09903-070 when the reaction is performed using BHT and MEHQ as inhibitors for the optimized improved large scale synthesis of Poloxamer 407DM of Example 3;
FIG. 40 is GC chromatogram of the batch FS-09903-070 when the reaction is performed using BHT and MEHQ as inhibitors for the optimized improved large scale synthesis of Poloxamer 407DM of Example 3. There are no detectable solvent residue, MAA, BHT and MEHQ in the final product.
FIG. 41 is the HPLC chromatogram showing the high molecular weight component identification of the batch FS-09903-070 when the reaction is performed using BHT and MEHQ as inhibitors for the optimized improved large scale synthesis of Poloxamer 407DM of Example 3;
FIG. 42 and FIG. 43 are GPC chromatograms of the batch FS-09903-070 when the reaction is performed using BHT and MEHQ as inhibitors for the optimized improved large scale synthesis of Poloxamer 407DM of Example 3. There is no detectable high molecular weight impurity.



DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
A poloxamer or poloxamine compound is reacted with a first methacrylating agent in the presence of a solvent stabilized with a first free radical inhibitor to form a first reaction product, and the first reaction product is further reacted with an organic base and a second methacrylating agent stabilized with a second free radical inhibitor to form a second reaction product, wherein the first free radical inhibitor is a different compound than the second free radical inhibitor. In various embodiments of the inventions, the first and second methacrylating agents may be the same, or may be different. In a specific embodiment, the first and second methacrylating agent is methacryloyl chloride. In embodiments wherein the poloxamer or poloxamine compound is a poloxamer compound, the first reaction product may be a monomethacrylated poloxamer compound, and the second reaction product may be a dimethacrylated poloxamer compound. In embodiments wherein the poloxamer or poloxamine compound is a poloxamine compound, the first reaction product may be a monomethacrylated poloxamine compound, and the second reaction product may be a polymethacrylated (e.g., di-, tri-, or tetra-methacrylated) poloxamine compound. The poloxamer or poloxamine compound may be reacted with the first methacrylating agent in the presence of the organic base, or the organic base may be added to the first reaction product after reaction thereof.
PEO- and PPO-containing block copolymers are presently preferred. One such copolymer that can be used with the method of the invention, is PLURONIC® F127, a block copolymer having the structure [(polyethylene oxide)99-(polypropylene oxide)66-(polyethylene oxide)99]. The terminal hydroxyl groups of the copolymer are functionalized with free radical polymerizable ethylenically unsaturated end groups to allow for the reaction of the PEO- and PPO-containing block copolymer with other device forming monomers.
A poloxamer compound such as PLURONIC F 127 is reacted with a methacrylating agent such as methacryloyl chloride while blanketed with nitrogen gas in the presence of a solvent such as anhydrous tetrahydrofuran (THF) stabilized with a free radical inhibitor such as 2,6 di tert-butyl-4-methyl phenol (BHT). After this portion of the synthesis has reacted, an organic base such as triethylamine (TEA) is added to the above solution with continued mixing. Alternatively, the organic base may be present during the first methacrylation reaction. To this reaction mixture is then added methacryloyl chloride which was stabilized with Hydroquinone monomethyl ether (MEHQ) with continued stirring under nitrogen gas. After the immediately above reaction has gone to completion the reaction mass is allowed to cool to room temperature (RT) and worked up to give the functionalized desired product as shown in the Reaction Scheme-1.




Turbidity measurements of PLURONIC F-127DM both in water and tetrahydrofuran were determined to be indicative of the presence of inorganic salts and any high molecular weight impurities. For the purpose of collecting the analytical data and to determine the desired product purity, dimethacrylation % conversion was checked by NMR & HPLC; GPC was used to determine the Mw and Mn molecular weights and GC was used to determine the residual solvents and methacrylic acid.
It is believed the formation of High Molecular Weight impurities in PLURONIC F-127DM is due to the inadvertent cross linking caused by the presence of undesirable radicals. The radicals formed in PLURONIC F-127DM chains can further react to form gel or High Molecular Weight impurities. This can be avoided by adding BHT as a free radical inhibitor. Any undesirable radical formed will react with BHT and result in a stabilized radical unable to react further thus avoiding gel formation. The two electron donating groups (t-butyl) in BHT also serve to activate the end terminal hydroxyl groups of the PEO-PPO containing block copolymer resulting in an increase of the efficiency of the inventive reaction.
Although not entirely certain, the inventors believe the efficacy of BHT is limited to suppression of radicals formed at only the propylene oxide repeating units of Poloxamer block copolymer. The reason for this limitation could be due to the sterically hindered stabilized radicals formed from BHT. It has surprisingly been discovered that the usage of a combination of MEHQ with BHT in the synthetic scheme of the invention herein can effectively stabilize the radicals formed at both the ethylene oxide and propylene oxide units of the poloxamer block copolymer.
Further, the radicals which are generated in the reaction medium due to use of highly polar solvents like THF at large scale reaction conditions are effectively stabilized by use of these two inhibitors.
Mechanism (Action of Inhibitor): Usage of BHT







Effective oxidation of BHT gives rise to mixture of compound which are called quinoid compounds. 3,5,3′,5′-tetratert-butylstilbiene-4,4′-quinone and 2,6-ditert-butyl-1,4-benzoquinone.
EXAMPLES
Example 1
Modified Process for Manufacturing of Poloxamer 407 DM (FS09458-036): with Single Inhibitor System (BHT as an Inhibitor)
Observations: When 2,6 ditert-butyl 4 methyl phenol (BHT) was used as an inhibitor, the High Molecular Weight component was minimized and the Turbidity of the product in THF and Water was very much reduced.
Typical Procedure: 500 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 3500 mL) stabilized with 5250 mg of 2,6 ditert-butyl-4-methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 68 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 47.5 mL of freshly distilled methacryloyl chloride at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up: The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 250 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 250 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 1165 mL of THF to get 2335 mL of mass. The mass was precipitated into 16000 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Analytical Characterization
% Conversion by 1H-NMR:
1H-NMR spectrum of FS09458-036 was recorded in DMSO-d6 on a Bruker Avance 400 MHz. The conversion was 82.3% (FIG. 1)
Calculation:
No. of protons at 6.1 ppm 1.63+No. of protons at 5.6 ppm 1.67/4=3.3/4×100=82.3%
Conversion by HPLC: Determination of Poloxamer 407 Dimethacrylate
(FS09458-036): The HPLC analysis was performed Agilent 1200 series HPLC system with SHODEX KB-804 (300×8) mm column a using SODIUM ACETATE BUFFER as Mobile Phase.
Calculation: % Conversion by HPLC (FIG. 2 to FIG. 9)








TABLE 1






Product: Poloxamer 407 Dimethacrylate



Study: % Conversion (By HPLC)



B. No.: FS09458-036



AR No.: A529453



Check standard weight (mg) = 203.4



Standard weight (mg) = 198.0



% Conversion for standard = 84.7





















Total peak



Peak-1
Peak-2
area





Check
2350798
1555236
3906034


standard





Standard
2226408
1329009
3555417



% Agreement

106.94










System precision:













Total peak


S. No.
Peak-1
Peak-2
area





1
2226408
1329009
3555417


2
2329288
1391799
3721087


3
2329679
1444988
3774667


4
2315375
1467507
3782882


5
2286721
1361211
3647932


6
2297159
1503218
3800377


Average
2297438.3
1416288.7
3713727.0


Std. Dev
38822.245
66715.38316
95340.99627


% RSD
1.69
4.71
2.57










% Conversion













Sample



%



weight


Total peak
Con-


S. No.
(g)
Peak-1
Peak-2
area
version





1
198.9
2593651
1595599
4189250.0
95.11


2
199.0
2614811
1693300
4308111.0
97.76


Average

2604231.00
1644449.50
4248680.50
96.44


Std.Dev

14962.38
69085.04
84047.42
1.87


% RSD

0.57
4.2
1.98
1.94









  
    %
    ⁢
    
      
    
    ⁢
    Conversion
  
  =
  
    
      
        
          
            Total
            ⁢
            
              
            
            ⁢
            sample
            ⁢
            
              
            
            ⁢
            area
            ⁢
            
              
            
            ×
            standard
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
            ×
          
        
      
      
        
          
            %
            ⁢
            
              
            
            ⁢
            Conversion
            ⁢
            
              
            
            ⁢
            of
            ⁢
            
              
            
            ⁢
            the
            ⁢
            
              
            
            ⁢
            standard
          
        
      
    
    
      
        
          
            Average
            ⁢
            
              
            
            ⁢
            total
            ⁢
            
              
            
            ⁢
            standard
            ⁢
            
              
            
            ⁢
            area
            ×
          
        
      
      
        
          
            Sample
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
          
        
      
    
  










GC analysis was performed using Agilent GC 6890N series equipped with G188 Head space Auto sampler. Nitrogen was the carrier gas with flame ionized detector.
GC Calculation (FIG. 10 to FIG. 13):







TABLE 2





RESIDUAL MONOMER REPORT


Product: Poloxamer


Batch No.: FS09458-036


A.R. No.: A529453







System suitability:









S. No.
Monomer
Wt.





1
THF
0.0923


2
IPA
0.102


3
MAA
0.2198


4
BHT
0.092















THF
IPA
MAA
BHT





STD-1
78.607
81.421
168.016
134.286


STD-2
79.75
80.485
164.034
133.905


STD-3
77.716
77.771
172.812
127.356


Average
78.691
79.89233333
168.2873333
131.849


Std. Dev
1.02
1.90
4.40
3.90


% RSD
1.30
2.37
2.61
2.95














Area of
% Residual













Mono-
Test
Test
Test
Test
Total


mer
sample-1
sample-2
sample-1
sample-2
Residual





THF
N.D
N.D
N.D
N.D
N.D


IPA
N.D
N.D
N.D
N.D
N.D


MAA
N.D
N.D
N.D
N.D
N.D


BHT
71.403
73.102
0.271365585
0.277822599
0.274594





Sample weight (g) = 0.0918






High Molecular Component was identified using HPLC System: Chromatogram is given below. It is found that HMW component is 0.35% was present in this sample (FIG. 14).
GPC:
GPC of FS-09458-036 was performed using Agilent 1200 Series with Addon GPC software. PEG-PPg was used as the GPC standards (FIG. 15).
Turbidity measurement of the Poloxamer 407 DM was measured in NTU units using HF Scientific Turbidity meter (Make): Model-Micro 100 Turbid meter. Solution was prepared in 10% water and THF. The turbidity was measured in water (3 NTU) and in THF (6 NTU).
Methacrylic acid content: Titration method was followed.
Comparative Example 1
Old Process for Manufacturing of Poloxamer 407 DM: without any Inhibitor
Observations: High Molecular Weight component was observed and the Turbidity of the product in THF and Water was very much high.
Typical Procedure:
500 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 3500 mL was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 68 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 47.5 mL of methacryloyl chloride which at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 250 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was concentrated to remove 1165 mL of THF to get 2335 mL of mass. The mass was precipitated into 16000 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Analytical Characterization
% Conversion by 1H-NMR:
1H-NMR spectrum of FS-09458-037 was recorded in DMSO-d6 on a Bruker Avance 400 MHz. The conversion was 87.7%. Calculation: No. of protons at 6.1 ppm 1.75+No. of protons at 5.6 ppm 1.76=3.51/4×100=87.7 (FIG. 16)
% Conversion by HPLC: Determination of Poloxamer 407 Dimethacrylate:
The HPLC analysis of FS-09458-037 was performed using Agilent 1200 series HPLC system with SHODEX KB-804(300×8) mm column a using SODIUM ACETATE BUFFER as Mobile Phase.
Calculation: % Conversion by HPLC (FIG. 17 to FIG. 24)








TABLE 3






Product: Poloxamer 407 Dimethacrylate



Study: % Conversion (By HPLC)



B. No.: FS09458-037



AR No.: A532877



Check standard weight (mg) = 202.1



Standard weight (mg) = 199.2



% Conversion for standard = 84.7





















Total peak



Peak-1
Peak-2
area





Check
2243804
1652655
3896459


standard





Standard
2242333
1744509
3986842



% Agreement

96.33










System precision:













Total peak


S. No.
Peak-1
Peak-2
area





1
2242333
1744509
3986842


2
2184706
1632958
3817664


3
2156436
1714115
3870551


4
2176410
1687458
3863868


5
2159765
1581961
3741726


6
2107640
1637102
3744742


Average
2171215.0
1666350.5
3837565.5


Std. Dev
43950.124
59879.62368
91935.65117


% RSD
2.02
3.59
2.4










% Conversion













Sample



%



weight


Total peak
Con-


S. No.
(g)
Peak-1
Peak-2
area
version





1
198.2
2234443
1709801
3944244.0
87.49


2
199.4
2213491
1619436
3832927.0
84.51


Average

2223967.00
1664618.50
3888585.5
86.00









  
    %
    ⁢
    
      
    
    ⁢
    Conversion
  
  =
  
    
      
        
          
            Total
            ⁢
            
              
            
            ⁢
            sample
            ⁢
            
              
            
            ⁢
            area
            ⁢
            
              
            
            ×
            standard
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
            ×
          
        
      
      
        
          
            %
            ⁢
            
              
            
            ⁢
            Conversion
            ⁢
            
              
            
            ⁢
            of
            ⁢
            
              
            
            ⁢
            the
            ⁢
            
              
            
            ⁢
            standard
          
        
      
    
    
      
        
          
            Average
            ⁢
            
              
            
            ⁢
            total
            ⁢
            
              
            
            ⁢
            standard
            ⁢
            
              
            
            ⁢
            area
            ×
          
        
      
      
        
          
            Sample
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
          
        
      
    
  










GC analysis was performed using Agilent GC 6890N series equipped with G188 Head space Auto sampler. Nitrogen was the carrier gas with flame ionized detector.
GC Calculation (FIG. 25 to FIG. 29):







TABLE 4





RESIDUAL MONOMER REPORT


Product: Poloxamer


Batch No.: FS09458-037


A.R. No.: A532877







System suitability:









S. No.
Monomer
Wt.





1
THF
0.1142


2
IPA
0.1146


3
MAA
0.2013


4
BHT
0.0923

















THF
IPA
MAA
BHT







STD-1
96.264
|85.868
139.706
119.094



STD-2
98.787
86.458
145.900
123.176



STD-3
97.908
86.339
142.939
128.183



Average
97.653
86.399
142.848
123.484



Std. Dev
1.28
0.08
3.10
4.55



% RSD
1.31
0.10
2.17
3.69















Area of
% Residual













Mono-
Test
Test
Test
Test



mer
sample-1
sample-2
sample-1
sample-2
Result





THF
10.626
10.825
0.0631
0.0643
0.0637


IPA
N.D
N.D
N.D
N.D
N.D


MAA
16.737
17.311
0.1198
0.1240
0.1219


BHT
N.D
N.D
N.D
N.D
N.D










Total Residual solvent (%)
0.1830
0.1883
0.19 





Sample weight (g) = 0.0984






High Molecular Component was Identified Using HPLC System:
Chromatogram is given below. It is found that HMW component is 0.55% was present in this sample (FIG. 30).
Turbidity measurement of the Poloxamer 407 DM was measured in NTU units using HF Scientific Turbidity meter (Make): Model-Micro 100 Turbid meter. Solution was prepared in 10% water and THF. The turbidity in water showed 5 NTU and in THF 200 NTU.
GPC:
GPC was performed using Agilent 1200 Series with Addon GPC software. PEG-PPg were used as the GPC standards (FIG. 31).
Methacrylic Acid Content:
Titration method was followed. The Methacrylic acid content: 0.002% (for this sample).







TABLE 5







Reaction performed using BHT as an inhibitor for the synthesis of Poloxamer 407 DM


Compilation of Poloxamer 407 DM with different concentrations of BHT




















BHT used



BHT level

Turbidity



SL.

Batch
for process

% conversion
% conversion
by GC method
GPC
In THF


No
Batch. No
size (g)
(ppm)
THF grade
by NMR
by HPLC
(ppm)
% of HMW
(NTU)
Yield (g)










SET-1

















1
FS09458-032
500
0
Dry
80
99.8
0
0.3
25.1
455


2
FS09458-033
500
500
Dry
83.5
92.3
10
0.2
125
460


3
FS09458-036
500
1500
Dry
85.77
96.4
40
0.4
10
455


4
FS09458-035
500
0
Commercial
85.0
94.4
5
0.1
57.4
460







SET-2

















1
FS09458-037
500
0
Dry
83.6
86
0
0.6
340
465


2
FS09458-038
500
500
Dry
81.7
87.1
15
0.8
50
460


3
FS09458-040
500
1500
Dry
81.9
93.01
60
0.7
12.7
470


4
FS09458-039
500
0
Commercial
85.6
89.7
10
0.4
500
475







SET-3

















1
FS09458-042
500
0
Dry
80.7
89.5
0
1.3
410
470


2
FS09458-043
500
500
Dry
80.7
84.3
10
0.6
832
475


3
FS09458-041
500
1500
Dry
82.4
92.5
120
0.5
22
475


4
FS09458-044
500
0
Commercial
83
88.8
0
0.6
82.3
470









Advantage of BHT:
The formation of undesirable High MW contaminant is minimized and inhibitor is not carried over with the reaction product when used as part of a device forming monomer mix.
Disadvantage of BHT:
Did not completely stop the formation of the High MW contaminant.
Limitations of BHT:
Because BHT radical is stable & its sterically hindered this cannot further react with the radicals




The activity of BHT radical is limited which cannot alone stop the formation of high molecular weight impurities, combination of inhibitor system was performed. The new inhibitor is MEHQ.




Mechanism (Action of Inhibitor): Usage of MEHQ











TABLE 6







Reaction performed using BHT &MEHQ as inhibitors for the synthesis of


Poloxamer 407 DM.













Inhibitor
GPC
GPC

GPC















SL. No
Descrip'n
Batch No.
MeHQ
BHT
% HMW
% HMW
NMR
% HMW


















1
Set-1
FS09903-023
1000 ppm
0
ND
ND
72.5
ND


2

FS09903-024

500
ND
ND
66
ND


3

FS09903-025

1000
ND
ND
71.25
ND


4

FS09903-026

1500
ND
ND
73.75
ND


1
Set-2
FS09903-030
2000 ppm
0
. . .
. . .
87.5
ND


2

FS09903-031

500
0.06
0.03
83.5
0.09


3

FS09903-032

1000
0.04
ND
75.25
ND


4

FS09903-033

1500
ND
. . .
67.5
ND


1
Set-3
FS09903-034
3000 ppm
0
ND
ND
93
0.04


2

FS09903-035

500
ND
ND
92
ND


3

FS09903-036

1000
ND
ND
81
ND


4

FS09903-037

1500
ND
0.06
95
ND


1
Set-4
FS09903-038
4000 ppm
0
ND
ND
85
ND


2

FS09903-039

500
. . .
ND
73
ND


3

FS09903-040

1000
ND
ND
61
ND


4

FS09903-041

1500
ND
ND
79.25
ND


1
Set-5
FS09903-042
5000 ppm
0
ND
. . .
91
ND


2

FS09903-043

500
ND
. . .
95
ND


3

FS09903-044

1000
ND
. . .
89.7
ND


4

FS09903-045

1500
ND
. . .
94.5
ND









The usage of MEHQ along with BHT shown no High molecular weight impurity in Poloxamer 407 DM








TABLE 7







Comparative data: BHT &MEHQ as a inhibitors


for the synthesis of Poloxamer 407 DM









BHT












0 ppm
500 ppm
1000 ppm
1500 ppm


MEHQ
% of
% of
% of
% of


level (ppm)
conversion
conversion
conversion
conversion





1000
73
66
71
74


2000
88
84
75
68


3000
93
92
81
95


4000
84
73
61
79


5000
91
95
90
95
















TABLE 8







Reaction performed using BHT &MEHQ as a inhibitors for the synthesis of Poloxamer 407 DM










GPC















HMW






Main Peak
Peak

GC (ppm)



















% Conversion


Area
Area
% HMW

BHT +

Turbidity
Titration





















Sl. No.
Batch No.
NMR
HPLC
Mw
Mn
(nRlu)
(nRlu)
(nRlu)
THF
MeHQ
n-Heptane
IPA
THF
MAA (%)
























1
FS09903-010
94
92.29
8815
7224
682531.68
ND
ND
ND
ND
ND
ND
3.69
0.0051


2
FS09903-011
91
90.58
8855
7447
805058.96
658.05
0.08
ND
ND
2779
ND
Not done
0.0034


3
FS09903-015
96
87.98
8842
7447
732759.25
ND
ND
ND
ND
2323
ND
7.05
0.0085


4
FS09903-016
87
94.59
8893
7461
799899.87
ND
ND
ND
ND
ND
ND
Not done
0.0032


5
FS09903-017
92
92.16
8784
7362
790396.46
ND
ND
ND
ND
ND
1062
Not done
0.0034


6
FS09903-018
92
93.31
8930
7443
805179.62
ND
ND
ND
ND
ND
ND
23.7
0.005


7
FS09903-019
100
90.98
9033
7579
700435.68
ND
ND
ND
ND
ND
ND
8.76
0.0052


8
FS09903-020
89
90.9
8930
7597
708862
ND
ND
ND
ND
ND
ND
29
0.0051









Example 2
Improved Synthesis of Functionalized Surfactants
100 g of PLURONIC F 127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF) (700 mL) stabilized with 1500 ppm of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 3000 ppm of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT. The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove one third volume of THF. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum. The NMR analysis of the final polymer showed greater than 89% conversion.
Example 3
Optimized Improved Large Scale Process for Manufacturing of Poloxamer 407 DM: with Double Inhibitor System (BHT and MeHQ as Inhibitors)
Observations: When 2,6 ditert-butyl 4 methyl phenol (BHT) & Hydroquinone monomethyl ether (MeHQ) was used as an inhibitor, the High Molecular Weight component was eliminated and the Turbidity of the product in THF and Water was drastically reduced
Typical Procedure:
1200 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 8400 mL) stabilized with 12600 mg of 2,6 ditert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 163.2 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 114 mL of freshly distilled methacryloyl chloride which was stabilized with 342 mg of Hydroquinone monomethyl ether (MeHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 600 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 600 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 2800 mL of THF to get 5600 mL of mass. The mass was precipitated into 32000 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Analytical Characterization
% Conversion by 1H-NMR:
1H-NMR spectrum of FS09903-070 was recorded in DMSO-d6 on a Bruker Avance 400 MHz. The conversion was 82.3% (FIG. 32). Calculation: No. of protons at 6.1 ppm 1.89+No. of protons at 5.6 ppm 1.92/4=3.81/4×100=95.2%
Conversion by HPLC: Determination of Poloxamer 407 Dimethacrylate
(FS09903-070): The HPLC analysis was performed on Agilent 1200 series HPLC system with SHODEX KB-804(300×8) mm column a using SODIUM ACETATE BUFFER as Mobile Phase.
Calculation: % conversion by HPLC (FIG. 33 to FIG. 39)








TABLE 9






Product: Poloxamer 407 Dimethacrylate



Study: % Conversion (By HPLC)



B. No.: FS09903-070



AR No.: A576683



Standard weight (mg) = 200.5



% Conversion for standard = 84.7















System precision:













Total peak


S. No.
Peak-1
Peak-2
area





1
5941334
2720732
8662066


2
6069470
2866854
8936324


3
5992173
2775991
8768164


4
5989656
2704351
8694007


5
6070184
2792707
8862891


6
6069636
2822317
8891953


Average
6022075.5
2780492.0
8802567.5


Std. Dev
55293.83487
61230.54855
111560.8785


% RSD
0.92
2.2
1.27










% Conversion













Sample



%



weight


Total peak
Con-


S. No.
(mg)
Peak-1
Peak-2
area
version





1
200.4
6389350
3407932
9797282.0
94.32


2
200.3
6407197
3519536
9926733.0
95.61


Average

6398273.50
3463734.00
9862007.5
94.97









  
    %
    ⁢
    
      
    
    ⁢
    Conversion
  
  =
  
    
      
        
          
            Total
            ⁢
            
              
            
            ⁢
            sample
            ⁢
            
              
            
            ⁢
            area
            ⁢
            
              
            
            ×
            standard
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
            ×
          
        
      
      
        
          
            %
            ⁢
            
              
            
            ⁢
            Conversion
            ⁢
            
              
            
            ⁢
            of
            ⁢
            
              
            
            ⁢
            the
            ⁢
            
              
            
            ⁢
            standard
          
        
      
    
    
      
        
          
            Average
            ⁢
            
              
            
            ⁢
            total
            ⁢
            
              
            
            ⁢
            standard
            ⁢
            
              
            
            ⁢
            area
            ×
          
        
      
      
        
          
            Sample
            ⁢
            
              
            
            ⁢
            weight
            ⁢
            
              
            
            ⁢
            
              (
              mg
              )
            
          
        
      
    
  










GC analysis was performed using Agilent GC 6890N series equipped with G188 Head space Auto sampler. Nitrogen was the carrier gas with flame ionized detector.







TABLE 10





RESIDUAL MONOMER REPORT


Product Poloxamer


Batch No. FS09903-070


A.R.No. A576683







System suitability:









S. No.
Monomer
Wt.





1
HEPTANE
0.0218


2
THF
0.0187


3
IPA
0.0187


4
MAA
0.0186


4
BHT
0.0194


5
MEHQ
0.0201

















HEPTANE
THF
IPA
MAA
BHT
MEHQ





STD-1
66.441
33.875
28.533
28.222
54.747
32.643


STD-2
66.139
33.923
28.318
27.711
54.686
35.663


STD-3
66.529
34.366
29.157
27.212
56.565
33.533


STD-4
65.676
33.979
28.818
27.631
56.005
37.052


STD-5
64.806
33.774
28.489
27.215
55.321
31.885


STD-6
64.033
33.563
28.329
26.93
55.382
34.797


Average
65.604
33.91333
28.60733
27.48683
55.451
34.262


Std. Dev
1.00
0.27
0.32
0.46
0.73
1.941


% RSD
1.52
0.78
1.14
1.68
1.31
5.66















AREA OF

% RESIDUALS














RESIDUALS
TS-1
TS-2
TS-1
TS-2







HEPTANE
N.D
N.D
N.D
N.D



THF
N.D
N.D
N.D
N.D



IPA
N.D
N.D
N.D
N.D



MAA
N.D
N.D
N.D
N.D



BHT
N.D
N.D
N.D
N.D



MEHQ
N.D
N.D
N.D
N.D












Total residuals

N.D
N.D












Average

N.D







*TS—Test sample



**N.D—Not detected



Weight of test sample-1 (g) = 0.1001



Weight of test sample-2 (g) = 0.1005






GC Calculation (FIG. 40):
High Molecular Component was Identified Using HPLC System:
Chromatogram is given below. It is found that HMW component is not detected in this sample (FIG. 41).
GPC:
GPC was performed using Agilent 1200 Series with Addon GPC software. PEG-PPg was used as the GPC standards (FIG. 42 and FIG. 43).
Turbidity measurement of the Poloxamer 407 DM was measured in NTU units using HF Scientific Turbidity meter (Make): Model-Micro 100 Turbid meter. Solution was prepared in 10% water and THF. The turbidity in water showed 5 NTU and in THF 4 NTU.
Methacrylic Acid Content:
Titration method was followed. The Methacrylic acid content: 0.001% (for this sample).
Comparative Example 3
Prior Art Synthesis of Functionalized Surfactants
6.00 g of PLURONIC F127 was placed in a round bottom flask and dried thoroughly via azeotropic distillation of toluene (100 ml). The round bottom flask was then fitted with a reflux condenser and the reaction was blanketed with Nitrogen gas. Anhydrous tetrahydrofuran (THF) (60 ml) was added to the flask and the reaction was chilled to 5° C. with 15 equivalents (based upon the hydroxyl end groups) of triethylamine (TEA) was added (2.0 ml). 1.4 ml of methacryloyl chloride (15 equivalents) was dropped into the reaction mixture through an addition funnel and the reaction mixture was allowed to warm to room temperature and then stirred overnight. The reaction mixture was then heated to 65° C. for 3 hours. Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was concentrated to a volume of around 355 mL and precipitated into cold heptane. Two further precipitations were performed to reduce the amount of TEA-HCl salt to less than 0.2% by weight. NMR analysis of the final polymer showed greater than 90% conversion of the hydroxyl end groups to the methacrylated end groups.
Example 4
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 5
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of hydroquinone (HQ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 6
100 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of phenothiazine (PTZ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 28.5 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 7
100 g of PLURONIC F-68 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 19.78 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 13.95 mL of freshly distilled methacryloyl chloride which was stabilized with 41.8 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 8
100 g of PLURONIC P-105 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 25.73 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 18 mL of freshly distilled methacryloyl chloride which was stabilized with 54 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 9
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of phenothiazine (PTZ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 10
100 g of PLURONIC L-101 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 43.9 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 30.84 mL of freshly distilled methacryloyl chloride which was stabilized with 92.5 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 11
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of phenothiazine (PTZ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of hydroquinone (HQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 12
100 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 28.5 mg of hydroquinone (HQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 13
100 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of phenothiazine (PTZ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 28.5 mg of hydroquinone (HQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 14
100 g of PLURONIC F-38 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 35.54 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 24.93 mL of freshly distilled methacryloyl chloride which was stabilized with 74.8 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 15
100 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of hydroquinone (HQ) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 28.5 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 16
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of phenothiazine (PTZ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 17
100 g of PLURONIC F-108 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.5 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 8.01 mL of freshly distilled methacryloyl chloride which was stabilized with 24 mg of hydroquinone (HQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 18
100 g of PLURONIC F-127 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 13.6 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 9.5 mL of freshly distilled methacryloyl chloride which was stabilized with 28.5 mg of phenothiazine (PTZ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 19
100 g of PLURONIC P-123 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 28.99 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 20.78 mL of freshly distilled methacryloyl chloride which was stabilized with 61.14 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum
Example 20
100 g of PLURONIC F-87 was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 di tert-butyl 4 methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 21.57 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 15.21 mL of freshly distilled methacryloyl chloride which was stabilized with 45.6 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up:
The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.
Example 21
100 g of Tetronic 1107 (Poloxamine) was placed in a round bottom flask fitted with reflex condenser and the reaction was blanketed with nitrogen gas Anhydrous tetrahydrofuran (THF; 700 mL) stabilized with 1050 mg of 2,6 ditert-butyl-4-methyl phenol (BHT) was added to the flask and heated to 30° C. under stirring till it formed a clear solution. 11.21 mL of triethylamine (TEA) was added to the above solution and the stirring continued. To this was added drop wise 7.81 mL of freshly distilled methacryloyl chloride which was stabilized with 23.42 mg of Hydroquinone monomethyl ether (MEHQ) at 30-32° C. through an addition funnel. The reaction mixture was heated to 63° C. upon stirring with nitrogen gas for 3 hours. Then the reaction mass was cooled for 12 h at RT.
Work Up: The Precipitated salt (TEA-HCl) was filtered from the reaction mixture and the filtrate was treated with 50 g of Basic alumina under stirring for 30 minutes and filtered. The filtrate was treated with 50 g of basic resin (PUROLITE) under stirring for 30 minutes and filtered through celite bed. The filtrate was concentrated to remove 233 mL of THF to get 467 mL of mass. The mass was precipitated into 3200 mL of n-heptane. The precipitate was filtered and dried at 45° C. under high vacuum.

## Claims

1. A method comprising reacting a poloxamer or poloxamine compound with a first methacrylating agent in the presence of a solvent stabilized with a first free radical inhibitor to form a first reaction product, and further reacting the first reaction product with an organic base and a second methacrylating agent stabilized with a second free radical inhibitor to form a second reaction product, wherein the second free radical inhibitor is hydroquinone monomethyl ether (MEHQ), and wherein the first free radical inhibitor is a different compound than the second free radical inhibitor.

2. The method of claim 1 wherein the first and second methacrylating agents are the same.

3. The method of claim 2 wherein the methacrylating agent is methacryloyl chloride.

4. The method of claim 3 wherein the first free radical inhibitor is 2,6 di tert-butyl-4-methyl phenol (BHT).

5. The method of claim 4 wherein the organic base is triethylamine (TEA).

6. The method of claim 1 wherein the first free radical inhibitor is 2,6 ditert-butyl-4-methyl phenol (BHT).

7. The method of claim 1 wherein the organic base is triethylamine (TEA).

8. The method of claim 1 wherein the poloxamer or poloxamine compound is a poloxamer compound, wherein the first reaction product is a monomethacrylated poloxamer compound and wherein the second reaction product is a dimethacrylated poloxamer compound.

9. The method of claim 5 wherein the poloxamer or poloxamine compound is reacted with the first methacrylating agent in the presence of the organic base.

10. The method of claim 1 wherein the poloxamer or poloxamine compound is a poloxamine compound, wherein the first reaction product is a monomethacrylated poloxamine compound and wherein the second reaction product is a polymethacrylated poloxamine compound.

11. The method of claim 10 wherein the first and second methacrylating agents are methacryloyl chloride, and the first free radical inhibitor is 2,6 di tert-butyl-4-methyl phenol (BHT).

12. The method of claim 11 wherein the organic base is triethylamine (TEA).

13. The method of claim 12 wherein the poloxamine compound is reacted with the first methacrylating agent in the presence of the organic base.

14. The method of claim 1 wherein the poloxamer or poloxamine compound is reacted with the first methacrylating agent in the presence of the organic base.

15. A method comprising reacting a monomethacrylated poloxamer or monomethacrylated poloxamine compound with an organic base and a methacrylating agent stabilized with a hydroquinone monomethyl ether (MEHQ) free radical inhibitor to form a dimethacrylated poloxamer compound or a polymethacrylated poloxamine compound.

16. The method of claim 15, wherein the monomethacrylated poloxamer compound is reacted with an organic base and a methacrylating agent stabilized with a hydroquinone monomethyl ether (MEHQ) free radical inhibitor to form a dimethacrylated poloxamer compound.

17. The method of claim 15, wherein the monomethacrylated poloxamine compound is reacted with an organic base and a methacrylating agent stabilized with a hydroquinone monomethyl ether (MEHQ) free radical inhibitor to form a polymethacrylated poloxamine compound.

18. A reaction mixture comprising a monomethacrylated poloxamer or monomethacrylated poloxamine compound, an organic base, a methacrylating agent, and a hydroquinone monomethyl ether (MEHQ) free radical inhibitor.

19. The reaction mixture of claim 18, wherein the monomethacrylated poloxamer or monomethacrylated poloxamine compound comprises a monomethacrylated poloxamer compound.

20. The reaction mixture of claim 18, wherein the monomethacrylated poloxamer or monomethacrylated poloxamine compound comprises a monomethacrylated poloxamine compound.