# Patent

## Title
Multi-bracted lavender plants

## Abstract

Lavandula plants in section Stoechas having at least two branched cymeous spikes are disclosed. The present invention further relates to methods for producing Lavandula section Stoechas plants having branched cymeous spikes, and methods of breeding Lavandula plants having branched cymeous spikes, wherein at least one parent plant is a Lavandula section Stoechas plant having branched cymeous spikes. Lavandula section Stoechas plants produced by the described methods and characterized by their unique branched cymeous spikes are also described.

## Background
BACKGROUND
Lavender is a flowering plant native to Africa, Europe, and Asia, and is cultivated in temperate climates for gardens and landscaping. The flowers may be varying shades of blue, purple, pink, yellow, and white.
A member of the mint family, Lamiaceae, the genus Lavandula comprises 40 species of annuals, perennials, evergreen shrubs, and herbs. This genus is categorized into three subgenera: subgenus Lavandula, subgenus Fabricia, and subgenus Sabaudia. The subgenera of Lavandula is further subcategorized into three sections: section Lavandula, section Dentatae, and section Stoechas. Species within section Stoechas may also be called French lavender, Italian lavender or Spanish lavender.
Section Stoechas has three species: L. stoechas, L. pedunculata, and L. viridis. Additionally, there are subspecies of L. stoechas and L. pedunculata, including subsp. stoechas, subsp. luisieri, subsp. pedunculata, subsp. cariensis, subsp. atlantica, subsp. lusitanica, and subsp. sampaiana. 
The foregoing examples of related art and limitations related therewith are intended to be illustrative and not exclusive, and they do not imply any limitations on the inventions described herein. Other limitations of the related art will become apparent to those skilled in the art upon a reading of the specification and a study of the drawings.



BRIEF DESCRIPTION OF THE FIGURES
The accompanying figures, which are incorporated herein and form a part of the specification, illustrate some, but not the only or exclusive, example embodiments and/or features. It is intended that the embodiments and figures disclosed herein are to be considered illustrative rather than limiting.
FIG. 1A is an illustration of an example simple cymeous spike of a Lavandula section Stoechas plant.
FIG. 1B is a photograph of a simple cymeous spike from 6 month old Lavandula stoechas. Some cymes have been removed to show the primary axis, the peduncle.
FIG. 1C is a photograph of a simple cymeous spike from 6 month old Lavandula pedunculata. Note the showy sterile bracts at the apex of the primary spike only.
FIG. 2A is an illustration of an example branched cymeous spike and bicincinnus cyme of the present disclosure.
FIG. 2B is a photograph of the new branched cymeous spike from 6 month old Lavandula stoechas. Some lateral spikes have been removed to show the primary axis, the peduncle.
FIG. 2C is a photograph of the new branched cymeous spike from 6 month old Lavandula pedunculata. Note the showy sterile bracts at the apex of both primary and lateral spikes.
FIG. 3 is a photograph of inflorescences of simple spikes compared to the branched cymeous spikes of the present disclosure.
FIG. 4A is a flow diagram of a breeding scheme that can be used to introduce the phenotype into L. pedunculata. 
FIG. 4B is a photograph of a L. pedunculata plant with branched cymeous spikes produced by the breeding scheme shown in FIG. 4A.
FIG. 5A is a flow diagram of a breeding scheme that can be used to introduce the phenotype into L. pedunculata. 
FIG. 5B is a photograph of a L. pedunculata plant with branched cymeous spikes produced by the breeding scheme shown in FIG. 5A.
FIG. 6A is a flow diagram of a breeding scheme that can be used to introduce the phenotype into L. pedunculata. 
FIG. 6B is a photograph of a L. pedunculata plant with branched cymeous spikes produced by the breeding scheme shown in FIG. 6A.
FIG. 7A is a flow diagram of a breeding scheme that can be used to introduce the phenotype into L. viridis. 
FIG. 7B is a photograph of a L. viridis plant with branched cymeous spikes produced by the breeding scheme shown in FIG. 7A.



DETAILED DESCRIPTION
Embodiments of the present disclosure may include Lavandula plants in section Stoechas having at least two branched cymeous spikes. Embodiments of the present disclosure may further comprise Lavandula plants in section Stoechas having branched cymeous spikes comprising between 15% and 100% of the total inflorescences of the plant as well as lateral spikes having one or more sterile bracts.
In a further embodiment of the present disclosure one or more methods for producing a Lavandula plant having branched cymeous spikes are provided. These methods may include, but are not limited to, crossing two Lavandula parent plants and harvesting the resultant seed, wherein at least one parent plant is a Lavandula section Stoechas plant having at least two branched cymeous spikes.
As used herein, the term “branched cymeous spike” refers to an inflorescence as having a primary axis, also known as the peduncle, with smaller lateral spikes arranged radially where one or more sterile bracts are produced at the terminus of each or most of the lateral spikes along with the apex of the peduncle. Each lateral spike has multiple bicincinnus cymes, usually having three to nine flowers each.
Lavandula plants in section Stoechas normally have an inflorescence that is commonly referred to as a simple spike. A simple spike consists of stalkless flowers arranged along an elongated, unbranched primary axis, the peduncle. This type of simple spike is also described as a cymeous spike, as flowers along the main axis are arranged in small clusters called cymes. Furthermore, Lavandula plants in section Stoechas are also characterized by large and colored sterile bracts borne at the apex of the spike which has a characteristic bunny ear appearance.
In FIG. 1A, a simple cymeous spike 100 is shown. As shown in FIG. 1A, FIG. 1B and FIG. 1C, a simple cymeous spike consists of stalkless flowers 109 arranged along an elongated, unbranched primary axis of the stalk of the inflorescence, known as the peduncle 101. This type of simple spike is also described as a cymeous spike, as the flowers 109 along the peduncle 101 are arranged in small clusters called bicincinnus cymes 103. As used herein, “bicincinnus cyme” 103 refers to a branching cyme having flowers 109 with a subtending bract 105 located at the base of each bicincinnus cyme 103. The subtending bracts 105 are leaf-like structures located at the node of the peduncle 101 and the bicincinnus cyme 103.
Furthermore, Lavandula plants in section Stoechas are also characterized by large, colored sterile bracts 107 borne at the apex of the peduncle 101 which have a characteristic bunny ear appearance. Sterile bracts are modified leaves produced in the inflorescence.
An example of a simple cymeous spike from a 6 month old Lavandula stoechas plant is shown in the photograph of FIG. 1B. Some cymes 103 have been removed to show the peduncle 101.
An example of a simple cymeous spike from a 6 month old Lavandula pedunculata plant having four sterile bracts is shown in FIG. 1C.
As will be discussed in further detail below and in relation to FIG. 1A, branched cymeous spikes are inflorescences that are differentiated from simple cymeous spikes due to the production of lateral spikes branching radially from the peduncle. The branched cymeous spike phenotype of the plants of the present disclosure has been shown through multiple generations of production to be stable and reproduce the branched cymeous spike phenotype. In an embodiment, Lavandula plants of the section Stoechas of the present disclosure produce inflorescences exhibiting the branched cymeous spike phenotype, an example range of inflorescences exhibiting the branched cymeous spike phenotype may range from 15% to 100% of the total inflorescences of the plant, with one or more sterile bracts at the terminus of each lateral spike, with a range of one to six sterile bracts per lateral spike.
The branched cymeous spike phenotype was discovered in 2000 as a branch mutation through a breeding program conducted by the inventor in Kulnura Australia. Cuttings of the branch mutation were taken and propagated vegetatively. Multiple generations exhibiting the branched cymeous spike were produced and a single clone of L. stoechas, exhibiting multiple branched cymeous spikes, was identified in 2005 and designated P2005. P2005 was shown to consistently produce branched cymeous spike inflorescences and has since been clonally propagated. Seed has been produced from several lines derived from line P2005 and plants produced from these crosses have been shown to reproduce the branched cymeous spike phenotype.
As shown in FIG. 2A, a branched cymeous spike 200 is provided. As discussed previously, an embodiment of the present disclosure relates to Lavandula plants in section Stoechas having branched cymeous spikes 200. As shown in FIG. 2A, FIG. 2B and FIG. 2C, a branched cymeous spike 200 is an inflorescence that is differentiated from a simple cymeous spike by having lateral spikes 201 arranged radially along the peduncle 101 (the primary axis) with sterile bracts 203 produced at the terminus of each lateral spike 201 in addition to the sterile bracts 107 produced at the apex of the peduncle 101.
As shown in FIG. 2A and FIG. 2C, at the apex of the peduncle 101 and each lateral spike 201 are terminal sterile bracts 107, 203 (please note, sterile bracts 203 are not shown on the lateral spike 201 in expanded view of FIG. 2A). In FIGS. 2A and 2C, two sterile bracts 107 are illustrated at the apex of the peduncle 101, but any number of sterile bracts, with a range of one to six, may be present. Therefore, while this example has two sterile bracts 107, it should be understood that this description is applicable to any Lavandula plant in section Stoechas having sterile bracts. As with the apex of the peduncle 101, each lateral spike 201 will generally contain at least one sterile bract 203.
Each lateral spike 201 has multiple bicincinnus cymes 103 generally having three to nine flowers 109 each. In the example expanded view of FIG. 2A, two bicincinnus cymes 103 are shown having three and five flowers 109, but any number, usually between three and nine may be present. Therefore, while this descriptive example has bicincinnus cymes 103 having three and five flowers 109, it should be understood that this description is applicable to any bicincinnus cyme 103 of a Lavandula section Stoechas plant. The subtending bracts 105, when present in branched cymeous spikes, are located at the node of the lateral spike 201 and the bicincinnus cyme 103.
As shown in FIG. 2B, an example of a branched cymeous spike from a 6 month old Lavandula stoechas plant is provided. Please note that some lateral spikes 201 have been removed to show the peduncle 101.
Another example of a branched cymeous spike from a 6 month old Lavandula pedunculata plant is shown in FIG. 2C.
In Tables 1-4, the plants of the disclosure are compared to commercial varieties ‘Bee Brilliant’, ‘Pukehou’ and ‘Lavender Lace’. Data was taken from 20 plants, aged 6 months old. Inflorescences were measured when fully mature but did not include any sterile bract discoloration. Weight of average inflorescence does not include the peduncle below the inflorescence. The abbreviation SD refers to the standard deviation. Significance values were obtained using a two-tail P test of equal variance for Table 2, and a two-tail P test of unequal variance for Table 3 and Table 4.
As shown in FIG. 3 and Table 1, the plants of the present disclosure have much larger inflorescences due to the presence of multiple lateral spikes. While simple cymeous spikes do not exhibit any branching, the plants of the present disclosure have an average number of 17.8 lateral spikes per inflorescence, with a range of 4 to 29. However, a range of 1 to 60 lateral spikes per inflorescence and all integers in between may be present.







TABLE 1







Number of Lateral Spikes










Standard
Branched



Simple Spike
Cymeous Spikes













0
21



0
29



0
19



0
19



0
20



0
18



0
16



0
15



0
17



0
4


SD
0
6.20


AVERAGE
0
17.8









As shown in Table 2, the plants of the present disclosure have inflorescences that are approximately double the width of standard simple spike, with average width of 56.8 mm compared to 29.1 mm, which equates to an average 95% increase. Plants of the present disclosure exhibit inflorescences ranging in width from 42 mm to 70 mm and all integers in between, but a wider range of values may be present.







TABLE 2







Individual Inflorescence Width (mm)










Standard
Branched



Simple Spike
Cymeous Spikes













42
60



35
50



16
56



15
55



30
42



35
70



36
70



31
60



25
55



26
50


SD
8.72
8.74


AVERAGE
29.1
56.8








% INCREASE
95%


P(T <= t) TWO-TAIL
1.30037E−06









As shown in Table 3, inflorescences of the present disclosure also have greater weight. Plants having a branching cymeous spike of the present disclosure have an average inflorescence weight of 4.35 grams, while the average commercial simple spike varieties have an average inflorescence weight of 1.57 grams, which equates to an average 177% increase. Plants of the present disclosure exhibit inflorescences ranging in weight from 2.70 g to 6.23 g and all integers in between, but a wider range of values may be present. The increased width and weight of the plants of the disclosure is due to the additional lateral spikes, which carry their own bicincinnus cymes, subtending bracts and sterile bracts. The plants of the disclosure also produce more flowers than Lavandula plants in section Stoechas having simple cymeous spikes, due to the additional bicincinnus cymes present on the lateral spikes (data not shown).







TABLE 3







Individual Inflorescence Weight(g)










Standard
Branched



Simple Spike
Cymeous Spikes













1.89
4.26



1.51
3.48



1.71
3.33



2.80
6.23



0.98
6.08



1.80
2.70



1.27
6.03



0.92
3.58



1.73
3.49



1.10
4.33


SD
0.56
1.30


AVERAGE
1.57
4.35








% INCREASE
177%


P(T <= t) TWO-TAIL
4.486E−05









As shown Table 4, plants exhibiting a branching cymeous spike have a 1,062% increase in sterile bracts per inflorescence, since each lateral spike, in addition to the primary spike, carry their own set of showy sterile bracts. Plants of the disclosure have inflorescences with an average of 60.4 sterile terminal bracts compared to an average of 5.2 sterile terminal bracts for simple spikes. Plants of the disclosure exhibit a range of 23 to 70 sterile bracts per inflorescence, but a range of 2 to 300 and all integers in between may be present.







TABLE 4







Number of Sterile Terminal Bracts per Inflorescence










Standard
Branched



Simple Spike
Cymeous Spikes













4
70



4
72



6
65



7
76



6
75



5
68



4
50



6
45



4
60



6
23


SD
1.14
16.69


AVERAGE
5.2
60.4








% INCREASE
1,062%


P(T <= t) TWO-TAIL
2.50572E−06









Embodiments of the present disclosure may also include a Lavandula section Stoechas inflorescence exhibiting a branching cymeous spike with between 1 and 60 lateral spikes.
Embodiments of the present disclosure further include a Lavandula section Stoechas inflorescence exhibiting a branching cymeous spike having a weight of between 2.5 grams and 6.5 grams.
Embodiments of the present disclosure further include a Lavandula section Stoechas inflorescence exhibiting a branching cymeous spike and having between 2 and 300 sterile terminal bracts.
Embodiments of the present disclosure further include a plant part, wherein said plant part comprises a cell, seed, protoplast, tissue culture, or vegetative cutting produced from Lavandula section Stoechas plants having branched cymeous spikes, and plants regenerated therefrom.
Embodiments of the present disclosure further include methods for producing Lavandula plants having at least two branched cymeous spikes. An example method may include crossing two Lavandula parent plants and harvesting the resultant seed, wherein at least one Lavandula parent plant is a Lavandula section Stoechas plant having branched cymeous spikes.
An additional method for producing Lavandula plants having branched cymeous spikes may include, but is not limited to, intraspecific hand hybridization. Using a Lavandula section Stoechas plant having branched cymeous spikes as a male or female parent, an initial cross is performed with a normal flowering Lavandula plant within section Stoechas or with a hybrid of section Stoechas. The resulting seeds are sown and the seedlings are grown according to conventional methods. The flowering F1 progeny are then scored for branched cymeous spikes and those selected may be further crossbred with siblings or half siblings, or backcrossed to the branched cymeous spiked parent, or self-pollinated, or outcrossed to another species. Additional crosses may be performed, and subsequent progeny (F2, F3, F4, etc.) may be further crossbred with siblings or half siblings, or backcrossed to the branched cymeous spiked parent, or self-pollinated, or outcrossed to another species. Chosen plants having branched cymeous spikes may then be clonally propagated to ensure stability of the trait.
As will be understood by one skilled in the art once they understand the scope of the embodiments, additional breeding methods may be used to produce Lavandula plants having at least two branched cymeous spikes as described herein. Example methods may also include, sib- and half-sib crossing, backcrossing, and outcrossing with other Lavandula section Stoechas to increase genetic diversity and, incorporate desirable traits, as well as prevent inbreeding depression.
The branched cymeous spike phenotype can be predictably bred into other Lavandula section Stoechas plants having different genetic backgrounds using the methods described hereinabove. Lavandula section Stoechas cultivars having the branched cymeous spike phenotype can be predictably selected in which the plants exhibit branched spikes comprising 15% to 100% of the total inflorescences and/or have an increased inflorescence size and weight. The degree of percentage of inflorescences, inflorescence biomass or inflorescence size can be predictably increased by means of recurrent selection. The branched cymeous spike phenotype can be predictably combined with other desirable Lavandula characteristics to produce commercially acceptable cultivars that can be stably reproduced by asexual propagation.
Embodiments of the present disclosure further include seeds, plants, and plant parts produced from crossing two Lavandula parent plants, wherein at least one parent plant is a Lavandula section Stoechas plant having at least two branched cymeous spikes.
Embodiments of the present disclosure include a Lavandula section Stoechas plant having branched cymeous spikes, wherein a representative sample of seed of said plant has been deposited under NCIMB Accession No. 42347.
Embodiments of the present disclosure further include seeds, plants, and plant parts produced from Lavandula section Stoechas plants having branched cymeous spikes, wherein a representative sample of seed of said Lavandula section Stoechas plant has been deposited under NCIMB Accession No. 42347.
As shown in Table 5, the genus Lavandula is divided into three subgenera, Lavandula, Fabricia, and Sabaudia. The subgenus Lavandula is divided into three Sections: section Lavandula, section Dentatae, and section Stoechas. Section Stoechas has three species: L. stoechas, L. pedunculata, and L. viridis. Additionally, there are subspecies of L. stoechas and L. pedunculata, including subsp. stoechas, subsp. luisieri, subsp. pedunculata, subsp. cariensis, subsp. atlantica, subsp. lusitanica, and subsp. sampaiana.







TABLE 5







Genus Lavandula taxonomy










Subgenus
Section
Species
Subspecies





Lavandula
Stoechas
L. stoechas 
subsp. stoechas





subsp. luisieri




L. pedunculate
subsp. pedunculata 





subsp. cariensis





subsp. atlantica





subsp. lusitanica





subsp. sampaiana




L. viridis
none



Lavandula
L. angustifolia
subsp. angustifolia





subsp. pyrenaica




L. latifolia
none




L. lanata
none



Dentatae
L. dentata
2 varieties


Fabricia
Pterostoechas
L. multifida
none




L. canariensis
7 subspecies*




L. minutolii
2 varieties




L. bramwellii
none




L. pinnata
none




L. buchii
3 varieties




L. rotundifolia
none




L. maroccana
none




L. tenuisecta
none




L. rejdalii
none




L. mairei
2 varieties




L. coronopifolia
none




L. saharica
none




L. antineae
subsp. antinae





subsp. marrana





subsp. tibestica




L. pubescens
none




L. citriodora
none



Subnudae
L. subnuda
none




L. macra
none




L. dhofarensis
subsp. dhofarensis





subsp. ayunensis




L. samhanensis
none




L. setifera
none




L. qishnensis
none




L. nimmoi
none




L. galgalloensis
none




L. aristibracteata
none




L. somaliensis
none



Chaetostachys
L. bipinnata
none




L. gibsonii
none



Hasikenses
L. hasikensis
none




L. sublepiodota
none


Sabaudia
Sabaudia
L. atriplicifolia
none




L. erythraeae
none





*7 subspecies of L. canariensis include subsp. canariensis, subsp. gomerensis subsp. palmensis, subsp. hierrensis, subsp. lancerottensis, and subsp. fuerteventurae.






The subgenus Fabricia is divided into four Sections: section pterostoechas, section Subnudae, section Chaetostachys, and section Hasikenses. Each section of Fabricia is further divided into species and occasionally, subspecies. Subgenus Sabaudia has one section, Sabaudia, which is divided into two species: L. atriplicifolia and L. erythraeae. 
Embodiments of the present disclosure further include methods for producing interspecific seeds, plants, and plant parts by from crossing two Lavandula section Stoechas plants, wherein at least one plant is a Lavandula section Stoechas plant having branched cymeous spikes and the other is selected from the group consisting of Lavandula pedunculata or Lavandula viridis. 
Embodiments of the present disclosure further include Lavandula section Stoechas interspecific hybrids having branched cymeous spikes, wherein said branched cymeous spikes comprise 15% to 100% of the total inflorescences on said hybrid plant, and wherein said inflorescences have between 1 and 60 lateral spikes.
Embodiments of the present disclosure further include seeds, plants, and plant parts produced from crossing two Lavandula section Stoechas plants, wherein at least one Lavandula plant is a Lavandula section Stoechas plant having at least two branched cymeous spikes and the other is selected from the group consisting of subsp. stoechas, subsp. luisieri, subsp. pedunculata, subsp. cariensis, subsp. atlantica, subsp. lusitanica, or subsp. sampaiana. 
Embodiments of the present disclosure further include methods for producing intersectional hybrids having branched cymeous spikes by altering the chromosome number of Lavandula section Stoechas plants having branched cymeous spikes, such that they are then favorable to crosses with other lavender plants, including those within section Lavandula and section Dentatae. As used herein, intersectional hybrid refers to a plant produced by crossing two Lavandula plants from different sections.
Embodiments of the present disclosure further include intersubgeneric hybrids having branched cymeous spikes that may be produced by altering the chromosome number of Lavandula section Stoechas plants having branched cymeous spikes, such that they are then favorable to crosses with other lavender plants, including those within the subgenus Fabricia and the subgenus Sabaudia. As used herein, intersubgeneric hybrid refers to plants produced by crossing two Lavandula plants from different subgenera.
EXAMPLES
The following examples are provided to illustrate further the various applications and are not intended to limit the invention beyond the limitations set forth in the appended claims.
Example 1
The Phenotype of Plants of the Present Disclosure
The branched cymeous spike phenotype described herein can be altered via standard growing techniques and environments to include inflorescences having only 1 lateral spike, so as to appear as a double spike, as well as those having up to 60 lateral spikes on a single inflorescence. Such inflorescences may have between 2 and 300 sterile bracts per inflorescence.
Example 2
Incorporating the Phenotype into Additional L. stoechas Lines
The branched cymeous spike phenotype as described herein can be bred into other L. stoechas lines having other flower colors and desirable characteristics. Crosses can be performed over several generations, and may include sib and half-sib crossing, backcrossing, and self-pollination. Plants may also be generated by protoplast fusion, a technique well known in the art. Plants can be selected for the branched cymeous spike phenotype as well as other desirable characteristics, including, but not limited to, flower color, oil content, drought tolerance, disease resistance, and pest resistance.
Example 3
Incorporating the Branched Cymeous Spike Phenotype into L. pedunculata 
As shown in FIG. 4A, the phenotype of the present disclosure was introduced into L. pedunculata through conventional breeding techniques. As outlined in flow diagram, a plant having branched cymeous spikes, P2005, was crossed with a proprietary line of L. stoechas, B01-12. The F1 progeny (SP 3385) was then backcrossed to the parental line having branched cymeous spikes, P2005. The F2 progeny, designated 3P, was outcrossed to a proprietary line of L. pedunculata designated 1038. The resultant F3 progeny was allowed to self-pollinate to produce an F4 progeny L. pedunculata plant with branched cymeous spikes that is stable and reproduces true to type.
Not shown in FIG. 4A but shown in FIG. 4B is a L. pedunculata plant having branched cymeous spikes produced using the flow diagram outlined in FIG. 4A.
Example 4
Incorporating the Branched Cymeous Spike Phenotype into L. pedunculata 
As shown in FIG. 5A, the phenotype of the present disclosure was introduced into L. pedunculata through conventional breeding techniques. As outlined in the flow diagram, plants exhibiting branched cymeous spikes, P2005, were crossed with two different proprietary lines of L. stoechas, designated B01-02 and B01-33. The F1 half-sibs, designated SP 651 and SP 657, were then intercrossed and the resultant F2 progeny, SP 3387, was outcrossed to a proprietary line of L. pedunculata, designated 1052. F3 progeny were allowed to self-pollinate to produce an F4 progeny L. pedunculata plant with branched cymeous spikes that is stable and reproduces true to type.
Not shown in FIG. 5A but shown in FIG. 5B is a L. pedunculata plant having branched cymeous spikes produced using the flow diagram outlined in FIG. 5A.
Example 5
Incorporating the Branched Cymeous Spike Phenotype into L. pedunculata 
As shown in FIG. 6A, the phenotype of the present disclosure was introduced into L. pedunculata through conventional breeding techniques. As outlined in the flow diagram, a plant exhibiting branched cymeous spikes, P2005, was crossed with a proprietary line of L. stoechas, B01-02. The F1 progeny was allowed to self-pollinate and the F2 progeny, SP 3387, was then outcrossed to a proprietary line of L. pedunculata, designated 1052. F3 progeny were allowed to self-pollinate to produce an F4 progeny L. pedunculata plant with branched cymeous spikes that is stable and reproduces true to type.
Not shown in FIG. 6A but shown in FIG. 6B is a L. pedunculata plant having branched cymeous spikes produced using the flow diagram outlined in FIG. 6A.
Example 6
Incorporating the Branched Cymeous Spike Phenotype into L. Viridis 
As shown in FIG. 7A, the phenotype of the present disclosure was introduced into L. viridis through conventional breeding techniques. As outlined in the flow diagram, a L. stoechas plant, designated COCWOB, was crossed with a proprietary line of L. viridis. The F1 progeny, designated 1050443, was then crossed to proprietary L. stoechas×L. pedunculata hybrid line exhibiting branched cymeous spikes, designated SP 1050440. The F2 progeny, designated 1161, was allowed to self-pollinate to produce an F3 progeny L. viridis plant with branched cymeous spikes that is stable and reproduces true to type.
Not shown in FIG. 7A but shown in FIG. 7B is a L. viridis plant having branched cymeous spikes produced using the flow diagram outlined in FIG. 7A.
Example 7
Generating Interspecific Hybrids
The phenotype of the present disclosure can be bred into interspecific hybrids. The subspecies of L. stoechas, subsp. stoechas and subsp. luisieri, may be bred together (L. stoechas subsp. stoechas×L. stoechas subsp. luisieri). Subspecies of L. pedunculata, including subsp. pedunculata, subsp. cariensis, subsp. atlantica, subsp. lusitanica, and subsp. sampaiana may be bred together. For example, L. pedunculata subsp. atlantica may be crossed with L. pedunculata subsp. pedunculata. Additionally, subspecies of L. stoechas and L. pedunculata may be bred together. For example, L.×cadevallii is a hybrid produced from crossing L. pedunculata subsp. pedunculata and L. stoechas, subsp. stoechas. 
Further still, the subspecies of L. stoechas and L. pedunculata may be crossed with L. viridis (L. pedunculata and L. stoechas×L. viridis hybrids). For example, L.×alportelensis is a hybrid produced from crossing L. stoechas subsp. luisieri and L. viridis. L.×limae is a hybrid produced from crossing L. pedunculata subsp. lusitanica and L. viridis. 
Example 8
Generating Intersectional Hybrids
In an embodiments of the present disclosure, intersectional hybrids having branched cymeous spikes may be produced by altering the chromosome number of Lavandula section Stoechas plants having branched cymeous spikes, such that they are then favorable to crosses with other Lavandula plants, including those within section Lavandula and section Dentatae. For example, an intersectional hybrid can be produced from crossing L. dentata (from section Dentatae) and L. latifolia (from section Lavandula). Methods of altering plant chromosome number may comprise using anti-miotic agents, which may include but is not limited to, colchicine, trifluralin, oryzalin, and amiprophos-methyl (APM), or may comprise irradiation. Methods of using colchicine or other anti-miotic agents are well known in the art, as discussed in Poehlman, J. M., Breeding Field Crops, University of Missouri, Hold, Rinehart and Winston Inc. (1996); Watts, L. Flower and Vegetable Plant Breeding, Grower Boods (1980); Callaway D. J. and Callaway M. B., Breeding Ornamental Plants, Timber Press Inc. (2000).
Example 9
Generating Intersubgeneric Hybrids
In an embodiments of the present disclosure, intersubgeneric hybrids having branched cymeous spikes may be produced by altering the chromosome number of Lavandula section Stoechas plants having branched cymeous spikes, such that they are then favorable to crosses with other Lavandula plants, including those within the subgenus Fabricia and the subgenus Sabaudia. Methods of altering plant chromosome number may comprise using anti-miotic agents, which may include but is not limited to, colchicine, trifluralin, oryzalin, and APM, or may comprise irradiation. Methods of using colchicine or other anti-miotic agents are well known in the art, as discussed in Poehlman, J. M., Breeding Field Crops, University of Missouri, Hold, Rinehart and Winston Inc. (1996); Watts, L. Flower and Vegetable Plant Breeding, Grower Boods (1980); Callaway D. J. and Callaway M. B., Breeding Ornamental Plants, Timber Press Inc. (2000).
Example 10
Clonally Propagating Lavandula Plants Having Branched Cymeous Spikes
In an embodiment of the present disclosure any Lavandula plant having branched cymeous spikes can be asexually propagated. Cuttings for asexual propagation can be taken at any time of the year from plants mature enough to provide cutting material, usually older than 3 months, and no special hormones or soil mixtures are used.
Lavandula plants may also be propagated by tissue culture. While a number of protocols exist and can be used to generate Lavandula plants by tissue culture, the following protocol may be used. Lavandula plants can be initiated in vitro by disinfection using sodium hypochlorite and shoot tips placed in test tubes containing modified Murashige and Skoog media. Clean (bacteria and fungus free) lab mother stock of Lavandula can be maintained and produced in vitro using modified Murashige and Skoog media supplemented with cytokinin to promote axillary branching. Cultures can be rooted on modified murashige and Skoog media supplemented with auxin to promote rooting.
While a number of exemplary aspects and embodiments have been discussed above, those of skill in the art will recognize certain modifications, permutations, additions and sub-combinations thereof. It is therefore intended that the following appended claims and claims hereafter introduced are interpreted to include all such modifications, permutations, additions, and sub-combinations as are within their true spirit and scope.
The foregoing discussion of the disclosure has been presented for purposes of illustration and description. The foregoing is not intended to limit the disclosure to the form or forms disclosed herein. In the foregoing Detailed Description for example, various features of the disclosure are grouped together in one or more embodiments for the purpose of streamlining the disclosure. This method of disclosure is not to be interpreted as reflecting an intention that the claimed disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the following claims are hereby incorporated into this Detailed Description, with each claim standing on its own as a separate preferred embodiment of the disclosure.
The use of the terms “a,” “an,” and “the,” and similar referents in the context of describing the disclosure (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having,” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. For example, if the range 10-15 is disclosed, then 11, 12, 13, and 14 are also disclosed. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the disclosure and does not pose a limitation on the scope of the disclosure unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the disclosure.
A representative sample of seed of Lavandula section Stoechas, including L. stoechas, L. pedunculata, L. viridis, and subspecies and interspecific hybrids within section Stoechas, where plants produced from the representative seed are capable of displaying the branched cymeous spikes phenotype of the present disclosure, has been placed on deposit under the Budapest Treaty with the National Collections of Industrial, Food and Marine Bacteria (NCIMB), Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen, Scotland, AB21 9YA, United Kingdom under NCIMB Accession No. 42347. The date of the deposit was Dec. 23, 2014. All restrictions upon availability to the public will be irrevocably removed upon granting of the patent in accordance with 37 C.F.R. §1.808(a)(2), and the deposit is intended to meet all of the requirements of 37 C.F.R. §1.801-1.809. The deposit will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced as necessary during that period.

## Claims

1. A Lavandula section Stoechas plant comprising the mutation contained in line P2005 that produces a branched cymeous spikes phenotype, wherein a representative sample of seed of said Lavandula section Stoechas plant containing said mutation that produces said phenotype having been deposited under NCIMB Accession No. 42347.

2. A seed produced from the Lavandula section Stoechas plant of claim 1.

3. A Lavandula section Stoechas plant comprising the mutation contained in line P2005 that produces a branched cymeous spike phenotype, wherein said plant is produced from seed deposited under NCIMB Accession No. 42347.

4. A Lavandula section Stoechas plant produced from the seed of claim 2, wherein between 15% and 100% of the inflorescences of the entire plant are branched cymeous spikes.

5. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype has branched cymeous spikes that comprise between 15% and 25% of the inflorescences of the entire plant.

6. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype has branched cymeous spikes that comprise between 26% and 35% of the inflorescences of the entire plant.

7. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype has branched cymeous spikes that comprise between 36% and 45% of the inflorescences of the entire plant.

8. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype has branched cymeous spikes that comprise between 46% and 55% of the inflorescences of the entire plant.

9. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype has branched cymeous spikes that comprise between 56% and 100% of the inflorescences of the entire plant.

10. A plant part produced from the plant of claim 1, wherein said plant part comprises a cell, seed, protoplast, tissue culture, or vegetative cutting.

11. A Lavandula section Stoechas plant regenerated from the cell, seed, protoplast, tissue culture, or vegetative cutting of claim 10, wherein said plant has said phenotype having at least two branched cymeous spikes.

12. The Lavandula section Stoechas plant of claim 1, wherein said branched cymeous spike has between 1 and 60 lateral spikes.

13. The Lavandula section Stoechas plant of claim 1, wherein the average weight of said branched cymeous spike has a weight of between 2.5 grams and 6.5 grams.

14. The Lavandula section Stoechas plant of claim 1, wherein said branched cymeous spike has between 2 and 300 terminal bracts.

15. The Lavandula section Stoechas plant of claim 1, wherein said plant having said phenotype is selected from the group consisting of Lavandula stoechas, Lavandula pedunculata, and Lavandula viridis.

16. A method for producing a Lavandula plant having a branched cymeous spikes phenotype, wherein the method comprises crossing two Lavandula parent plants and harvesting the resultant seed, wherein at least one parent plant is a Lavandula section Stoechas plant comprising the mutation contained in line P2005 that produces a branched cymeous spike phenotype, wherein said branched cymeous spikes comprise between 15% and 100% of the inflorescences of the entire plant, wherein a representative sample of seed of said Lavandula section Stoechas plant containing said mutation having been deposited under NCIMB Accession No. 42347, and wherein the second parent plant is selected from the group consisting of Lavandula stoechas, Lavandula pedunculata, or Lavandula viridis.

17. A Lavandula seed produced from the method of claim 16.

18. A Lavandula plant, or part thereof, having said branched cymeous spikes phenotype produced by growing the seed of claim 17, wherein said plant has branched cymeous spikes that comprise between 15% and 100% of the inflorescences of the entire plant.

19. The method of claim 16, wherein said second parent plant is a subspecies of Lavandula stoechas, or Lavandula pedunculata, and is selected from the group consisting of subspecies stoechas, subspecies luisieri, subspecies pedunculata, subspecies cariensis, subspecies atlantica, subspecies lusitanica, or subspecies sampaiana.

20. The method of claim 16, wherein said parent plants are Lavandula stoechas and further comprising:
backcrossing the F1 progeny to said parent having said branched cymeous spike phenotype and producing an F2 progeny;
outcrossing the F2 progeny to a Lavandula section Stoechas plant and producing an F3 progeny;
self-pollinating the F3 progeny; and
producing a Lavandula section Stoechas plant having a branched cymeous spike phenotype having two or more branched cymeous spikes.

21. The method of claim 20, wherein said F1 progeny is self-pollinated.

22. The method of claim 20, wherein said F1 progeny are intercrossed.

23. The method of claim 20, wherein said F2 progeny are outcrossed to Lavandula pedunculata.

24. The method of claim 20, wherein said F2 progeny are outcrossed to Lavandula viridis.

25. The method of claim 16, wherein an interspecific hybrid having said branched cymeous spike phenotype is produced, wherein said branched cymeous spikes comprise between 15% and 100% of the total inflorescences on said hybrid plant.