ECANA and ECANA Mimic Page 1 of 60

Biopesticides Registration Action Document

BIOPESTICIDES REGISTRATION ACTION DOCUMENT

Extract of Chenopodium ambrosioides near ambrosioides (ECANA)

(PC Code 599995)

and

ECANA Mimic (Extract of Chenopodium ambrosioides near 

ambrosioides Mimic: A blend of biochemical ingredients that is 

substantially similar to the Extract of Chenopodium ambrosioides near 

ambrosioides in composition.) 

(PC Code 179102)

U.S. Environmental Protection Agency

Office of Pesticide Programs

Biopesticides and Pollution Prevention Division
ECANA and ECANA Mimic Page 2 of 60

Biopesticides Registration Action Document

TABLE OF CONTENTS

I. BACKGROUND.......................................................................................................................... 4

II. OVERVIEW ................................................................................................................................ 5

III. REGULATORY BACKGROUND.............................................................................................. 6

A. Classification.......................................................................................................................... 7

B. Food Clearances/Tolerances................................................................................................... 7

IV. RISK ASSESSMANT.................................................................................................................. 7

A. Active Ingredient Characterization ........................................................................................ 7

B. Human Health Assessment ................................................................................................... .8

1. Toxicology ......................................................................................................................... 8

2. Effects on the Endocrine System....................................................................9

3. Dose Response Assessment ..............................................................................................10

4. Food Quality Protection Act Considerations...................................................................10

C. Environmental Assessment .................................................................................................. 12

1. Nontarget Organism Toxicology..................................................................................... 12

2. Environmental Fate and Ground Water Data................................................................. 12

3. Ecological Exposure and Risk Characterization ............................................................ 13

4. Endangered Species Statement........................................................................................ 13

D. Efficacy Data ........................................................................................................................ 14

V. RISK MANAGEMENT DECISION.......................................................................................... 14

A. Determination of Eligibility for Registration ....................................................................... 14

B. Regulatory Decision .............................................................................................................. 15

C. Environmental Justice...................................................................................16

VI. TERMS AND CONDITIONS OF THE REGISTRATION ..................................................... 16

VII. APPENDIX A.: DATA REQUIREMENTS (40 CFR PART 158)................................17

VIII. APPENDIX B: PRODUCT SPECIFIC INFORMATION..........................................24

IX. APPENDIX C: REFERENCES........................................................................25
ECANA and ECANA Mimic Page 3 of 60

Biopesticides Registration Action Document

BIOPESTICIDES REGISTRATION ACTION DOCUMENT TEAM

Office of Pesticide Programs:

Biopesticides and Pollution Prevention Division

Biochemical Pesticides Branch (BPB)

Chris Pfeifer Regulatory Action Leader

Angela Gonzales Science Reviewer

Kent Carlson, Ph.D. Biologist

Linda A. Hollis Branch Chief

Driss Benmhend Team Leader

Russell S. Jones, Ph.D. Senior Biologist
ECANA and ECANA Mimic Page 4 of 60

Biopesticides Registration Action Document

I. Background:

Extract of Chenopodium ambrosioides near ambrosioides (ECANA) is classified as a biochemical 

technical grade of the active ingredient (TGAI). The extract is derived from the flowering plant 

Chenopodium ambrosioides near ambrosioides, commonly known as American Wormseed. 

ECANA is a mix of constituent terpenes (biochemicals), most of which are also active ingredients 

already registered with the Agency. ECANA is intended for use as an insecticide and acaricide 

(mite and tick pesticide) on food crops and commercial ornamental crops. The extract is reported 

to work through a “nontoxic” mode of action by temporarily softening an insect’s cuticles, which 

then leads to a disruption in respiration.

ECANA Mimic is a blend of individual terpenes that is substantially similar to the natural extract 

in composition and effect. Product chemistry data confirm the extract’s structural similarity; and 

toxicology and efficacy data confirm the equivalency of its effects. Based on assessment of the 

data submitted in support of ECANA and ECANA Mimic, the two active ingredients are 

effectively considered to have the same risk profile, and shall be generally referred to as ECANA, 

except in cases where assessment of specific ECANA Mimic data warrants attention. 

Product chemistry data requirements (product identity, product analysis, manufacturing process, 

and physical/chemical properties), mammalian toxicology data requirements (acute oral, acute 

dermal, acute inhalation, dermal irritation, ocular irritation, hypersensitivity, 90-day oral, dermal 

and inhalation, prenatal development, and immunotoxicology), and ecological toxicity data 

requirements (avian acute oral and dietary, freshwater fish LC50, freshwater invertebrate LC50, 

nontarget plant toxicity, nontarget plant toxicity) were fulfilled by acceptable guideline studies and 

waiver rationales for both ECANA and ECANA Mimic.

No significant issues were identified for dietary risk, residential risk, or ground and surface water 

contamination from the use of ECANA as an active ingredient. Some risks from the use of 

ECANA and ECANA Mimic as an active ingredient were identified for occupational professions. 

Risks to these populations, however, were acceptably mitigated through the inclusion of additional 

personal protective equipment (PPE).

Some risks from the use of ECANA and ECANA Mimic as an active ingredient were identified for 

threatened and endangered species. These risks were acceptably mitigated through label provisions 

requiring spray buffers and an application interval for rain events.

The Agency’s risk management decision regarding the registration of ECANA also notes the 

following: 1) the active ingredient is naturally occurring; 2) pesticidal applications of the extract 

are applied directly to crops and are limited to 10 applications per year; 3) data indicate the extract 

has a very low mammalian toxicity; and 4) pesticide residues resulting from the intended method 

of application have been shown to rapidly degrade in terrestrial environments.

EPA considered ECANA in light of the relevant safety factors in the Food Quality Protection Act 

(FQPA) of 1996, and under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA). The 
ECANA and ECANA Mimic Page 5 of 60

Biopesticides Registration Action Document

Agency has not identified any dietary or non-dietary exposure issues that may affect the U.S. 

population in general, including infants and children. EPA, thereby, determined that there is 

reasonable certainty that no harm will result from aggregate exposure to ECANA residues, 

including dietary exposures and all other sources of exposure.

Consistent with the new policy of making registration actions more transparent, ECANA Mimic is 

subject to a 30 day comment period as a “new active ingredient” whose registration would result in 

a “first outdoor use” and a “first food use.” While a final decision on registration is contingent 

upon review and consideration of public comments, EPA presently believes that based on the risk 

assessment and information submitted in support of the registration of ECANA Mimic that it is in 

the best interests of the public and the environment to issue the registration for ECANA Mimic. 

The basis for this preliminary decision can be found in the risk assessment for ECANA Mimic, 

which is characterized in this BRAD. As discussed above, acute toxicity data for ECANA Mimic

indicate a nontoxic profile for acute toxicity. ECANA Mimic does not demonstrate subchronic 

toxicity, and it is not mutagenic or genotoxic. The toxicological endpoint for developmental 

toxicity for occupational users is not expected to pose a significant risk as users are required to 

wear personal protective equipment and exposures to ECAN Mimic are expected to be virtually

non-existent because of ECANA Mimic’s rapid rate of degradation. EPA has no concerns for any

nontarget organisms exposed to ECANA Mimic in accordance with approved label directions. 

EPA has not identified any toxic endpoints for nontarget mammals, birds, plants, or soil organisms.

While some aquatic nontarget toxicity has been identified, no risks are expected because 

applications are terrestrial, ECANA Mimic biodegrades rapidly, and label directions further negate 

the potential for aquatic exposure. Because of this lack of exposure, there are no concerns for any

threatened and endangered species. Thus, given that ECANA Mimic has very low toxicity, and 

presents little if any risk to nontarget organisms, and data confirm its effectiveness as a contact 

insecticide and acaricide, EPA concludes that it is in the best interests of the public and the 

environment to issue the registration for ECANA Mimic. 

II. ACTIVE INGREDIENT OVERVIEW

Common Name: American Wormseed Oil, Chenopodium Oil, ECANA, and ECANA 

Mimic 

Chemical Names: Extract of Chenopodium ambrosioides near ambrosioides and 

Extract of Chenopodium ambrosioides near ambrosioides Mimic 

Trade & Other Names: QRD 406 and QRD 420

CAS Registry Number: CAS# 89997-47-7 for ECANA; No CAS for ECANA Mimic

OPP Chemical Code: 599995 for ECANA; 179102 for ECANA Mimic

 Basic Manufacturer: AgraQuest, Inc. 

1540 Drew Avenue 
ECANA and ECANA Mimic Page 6 of 60

Biopesticides Registration Action Document

Davis, CA 95618

Type of Pesticide: Biochemical insecticide and acaricide (mite and tick pesticide) for 

food crops and commercial ornamental crops

Use Sites: Applied to food and ornamental crops. It has outdoor and greenhouse 

use sites.

Target Pests: Control of aphids, plant-feeding mites, whiteflies, mealy bugs, 

leafminers, thrips, fungus gnats, and tarnished plant bugs.

Formulation Type: Liquid

Method and Rates 

of Application: Applied with commonly used ground equipment, hose-end, 

pressurized sprayers and hand-held sprayers. It is diluted with water 

to make a sprayable solution. Upper and lower leaves are sprayed to 

the point of saturation. It is applied on a 7- to 14-day schedule as 

necessary. 

Timing: Applications should begin at first sign of insect pressure or plantfeeding mite infestation.

Use Practice 

Limitations: Not to be applied more than 10 times per year. Not to be used on 

flowering Cyclamen, Gerbera, Begonia, and Impatiens because it can 

cause phytotoxicity in those plants.

III. REGULATORY BACKGROUND

On February 25, 2005, the Agency received an application filed by Codena, Inc., Saint-Charlessur-Richelieu, Quebec, Canada, JOH 2GO (submitted by Landis International, Inc., P.O. Box 5126, 

Valdosta, GA 31603-5126) to register a manufacturing use product containing 100% of the new 

biochemical pesticide active ingredient, Extract of Chenopodium ambrosioides near ambrosioides 

(PC Code 599995, EPA Reg.# 81978-R) and two related end use products (EPA Reg.# 81978-E 

and EPA Reg.# 81978-G). A notice of receipt for these applications was published in the Federal 

Register May 18, 2005 (Volume 70, Number 95). Codena, Inc. was purchased by AgraQuest, Inc. 

(1520 Drew Ave., Davis, CA, 95618) on January 9, 2006; EPA Registration Numbers were 

subsequently transferred to reflect the new Company Number 69592. The manufacturing use 

product containing 100% Extract of Chenopodium ambrosioides near ambrosioides was assigned 

EPA Reg. # 69592-21. In the course of registration, one of the end use products was withdrawn. 

The remaining use product, which contains 25% of the active ingredient, was assigned EPA Reg.# 

69592-22.
ECANA and ECANA Mimic Page 7 of 60

Biopesticides Registration Action Document

On July 31, 2008, EPA issued a notice (Volume 73, Number 148) announcing the filing of a 

pesticide tolerance petition (PP 7F7299) by AgraQuest, Inc., 1540 Drew Avenue, Davis, CA, 

95618. On January 7, 2009, (Volume 74, Number 4) EPA issued a Final Rule establishing an 

exemption from the requirement of a tolerance for the residues of Extract of Chenopodium 

ambrosioides near ambrosioides when used as an insecticide/acaricide on all food commodities (40

CFR Section 180.1287).

On December 18, 2008, the Agency received an application filed by AgraQuest, Inc., 1520 Drew 

Ave., Davis, CA, 95618, to register a manufacturing use product containing 100% of the new 

biochemical pesticide active ingredient, ECANA Mimic (PC Code 179102, EPA Reg.# 69592-EU) 

and a related end use product (EPA Reg.# 69592-EL). AgraQuest, Inc. also petitioned the Agency

to amend ECANA’s exemption from the requirement from tolerance to include ECANA Mimic

(Pesticide Petition (PP) # 9F7551). A notice of receipt for these applications was published in the 

Federal Register on July 22, 2009 (Volume 74, Number 139). On May 6, 2009, EPA issued a 

notice (Volume 74, Number 86) announcing the filing of a pesticide tolerance petition (PP 

9F7551) by AgraQuest, Inc., 1540 Drew Avenue, Davis, CA, 95618. No comments were received 

in response to either notice of ECANA Mimic. This risk assessment (BRAD) was posted for 

comment on April 30, 2010.

A. Classification

On November 3, 2004, the Biochemical Classification Committee classified the Extract of 

Chenopodium ambrosioides near ambrosioides would be classified as a biochemical pesticide due 

to its apparent nontoxic mode of action. The extract works through a physical mode of action by

softening the cuticles of some insects, resulting in a disruption of respiration. In November of 

2008, the Biochemical Classification Committee also classified ECANA Mimic as a biochemical 

active ingredient.

B. Food Clearances/Tolerances

An exemption from the requirement of a tolerance is established for the residues of Extract of 

Chenopodium ambrosioides near ambrosioides when used as an insecticide/acaricide on all food 

commodities (40 CFR Section 180.1287). This risk assessment supports the amendment of this 

exemption to include ECANA Mimic. In either case, there are no CODEX maximum residue 

levels established for these active ingredients.

IV. RISK ASSESSMENT

A. Active Ingredient Characterization (40 CFR § 158.2030)

ECANA is a blended extract product comprised of numerous constituent compounds. Three 

primary constituent compounds (terpenes) have been identified as product markers. The balance of 

the constituents, present in small percentages and not commonly known to have toxic properties, is
ECANA and ECANA Mimic Page 8 of 60

Biopesticides Registration Action Document

considered as impurities. The active ingredients are produced via a distillation process, and the 

impurities in the product are inherent in the active ingredient. ECANA Mimic is a proportional 

blend of the terpene markers, which is substantially similar to ECANA. Product Chemistry data 

affirm the proportionality, and toxicology and efficacy data confirm the similar effect. 

Refer to Table 1 in Appendix A for a summary of product chemistry data requirements for 

ECANA. Refer to Table 2 in Appendix A for the summary of physical and chemical characteristics 

for ECANA; and refer to Table 3, 4, and 5 for the terpene marker in ECANA Mimic.

B. Human Health Assessment (40 CFR § 158.2050)

Adequate mammalian toxicology data/information is available to support registration of ECANA 

and ECANA Mimic. All toxicology data requirements have been satisfied. Refer to Table 6 in 

Appendix A for a summary of the toxicity information on ECANA; refer to Table 7 for the acute 

toxicity information associated with ECANA Mimic. 

.

1. Toxicity

For acute toxicity, ECANA is categorized as Toxicity Category III for acute oral and primary eye. 

Acute dermal, acute inhalation and primary dermal are categorized as Toxicity Category IV. 

ECANA is classified as a dermal sensitizer. ECANA Mimic showed similar acute toxicity, but 

had reduced primary eye toxicity and registered as a Toxicity Category IV; it was also found not to 

be a sensitizer.

Genotoxicity studies showed ECANA to be non-mutagenic and to pose no significant hazard with 

regard to genotoxicity. Cited literature also illustrated common exposure to each of the major 

marker constituents without observed teratogenic effect. Common exposures without observed 

effect included: (1) common consumption of the marker constituents in our fruits and vegetables; 

(2) regular dermal exposure to the marker constituents through the use of many cosmetics and 

generalized contact with plants; and (3) the natural presence of the marker constituents in much of 

the air that we breathe, particularly in spaces rich in vegetation.

The Agency accepted waiver rationales for the 90-Day Feeding Study, the 90-Day Dermal Study, 

the 90-Day Inhalation Study, and the Immune Response requirement. The waivers were accepted 

because no repeated or subchronic exposures were expected, given the use patterns for this 

product. The following points were also considered: (1) Repeated human exposure by the oral 

route is not anticipated for ECANA end-use products; (2) Opportunity for exposure is small, given 

the limited number of applications and the commercial nature of the use sites; (3) A residue decline 

study showed that the marker components of the active ingredient were not detectable 10 minutes 

after application to primrose leaves.; (4) A study where ECANA was applied four times at 2X the 

label rate on tomatoes found that the residues of marker components were below the limit of what 

can be measured (0.01 mg/kg) at 0 to 24 hrs post-treatment. (5) The marker ingredients have all 

been approved by FDA for use as food additives, albeit in limited fashion. 
ECANA and ECANA Mimic Page 9 of 60

Biopesticides Registration Action Document

2. Effects on the Endocrine System

As required under FFDCA section 408(p), EPA developed the Endocrine Disruptor Screening 

Program (EDSP) to determine whether certain substances (including pesticide active and other 

ingredients) may have an effect in humans or wildlife similar to an effect produced by a “naturally

occurring estrogen or other such endocrine effects as the Administrator may designate.” The 

EDSP employs a two-tiered approach to making the statutorily required determinations. Tier 1 

consists of a battery of 11 screening assays to identify the potential of a chemical substance to 

interact with the estrogen, androgen, or thyroid (E, A, or T) hormonal systems. Chemicals that go 

through Tier 1 screening and are found to have the potential to interact with E, A, or T hormonal 

systems will proceed to the next stage of the EDSP where EPA will determine which, if any, of the 

Tier 2 tests are necessary based on the available data. Tier 2 testing is designed to identify any

adverse endocrine related effects caused by the substance and establish a dose-response 

relationship between the dose and the E, A, or T effect.

Between October 2009 and February 2010, EPA is issuing test orders/data call-ins for the first 

group of 67 chemicals, which contains 58 pesticide active ingredients and 9 inert ingredients. This 

list of chemicals was selected based on the potential for human exposure through pathways such as 

food and water, residential activity, and certain post-application agricultural scenarios. This list 

should not be construed as a list of known or likely endocrine disruptors.

ECANA and its constituent markers are not among the group of 58 pesticide active ingredients on 

the initial list to be screened under the EDSP. Under FFDCA sec. 408(p), the Agency must screen 

all pesticide chemicals. Accordingly, EPA anticipates issuing future EDSP test orders/data call-ins 

for all pesticide active ingredients. 

For further information on the status of the EDSP, the policies and procedures, the list of 67 

chemicals, the test guidelines and the Tier 1 screening battery, please visit our website: 

http://www.epa.gov/endo/.

All available information supports the determination that ECANA is not an estrogenic, antiestrogenic, androgenic, or anti-androgenic compound. ECANA’s constituent markers are naturally

occurring compounds that are present in all of our fruits and vegetables. To date, there is no 

evidence to suggest that our natural exposure to ECANA’s markers affect the immune system, 

function in a manner similar to any known hormone, or that they act as endocrine disruptors. 

Moreover, the use of ECANA is not expected to result in any significant exposures, effectively

obviating any opportunity for negative effects on humans or the environment. Therefore, it is 

unlikely that ECANA would have estrogenic or endocrine effects. The Endocrine Disruption 

Screening Program (EDSP) has established a protocol, which guides the Agency in selecting 

suspect ingredients for review; and the Agency reserves the right to require new information,

should the program require it. Presently, based on the lack of exposure and the negligible toxicity

profile of the extract and its mimic, no adverse effects to the endocrine or immune systems are 

known or expected. 
ECANA and ECANA Mimic Page 10 of 60

Biopesticides Registration Action Document

3. Dose Response Assessment

A developmental toxicity study of ECANA established developmental toxicity endpoints of 0.60 

mg/kg-day for maternal exposures and 0.30 mg/kg-day for developmental exposures (DEEMFCID analyses). The endpoints were derived using data on the constituent marker of the most 

toxicological significance, and they are considered conservative. The Lowest Observed Adverse

Effect Level (LOAEL) for maternal exposures was 125 mg/kg-day, and 60 mg/kg-day for 

developmental exposure based on abnormal ossification, increases in skeletal abnormalities, and 

kidney weights. The endpoints do not trigger chronic testing, in this case, because significant 

exposure to ECANA or ECANA Mimic is precluded by its rapid degradation and the requirement 

for personal protective equipment (PPE). 

4. Food Quality Protection Act (FQPA) Considerations: Dietary Exposure and Risk 

Characterization

Dietary exposure to the residues of the contact insecticide Extract of Chenopodium ambrosioides

near ambrosioides, through food or water, is expected to be virtually nonexistent. And even in 

the event of exposure, the information on file with the Agency suggests that any dietary risks 

would be negligible.

Three residue studies demonstrate that the rapid degradation of ECANA leaves no opportunity for 

post-application exposure. A residue decline study on primrose demonstrated that, when the 

extract is sprayed at 4X the proposed application rate, residues of the three major active 

ingredient components declined to non-detectable levels within 10 minutes (MRID (Master 

Record Identification) 47209101). A second study on tomatoes involving four applications of the 

extract within a 24-hour period found no detectable residues on any samples collected 

immediately following the final application (MRID 46858903). A third residue study on mustard 

greens involving three applications at twice the application rate found no detectable residues 

within the hour after the third application (MRID 47548301). Essentially, data demonstrate that,

by the time ECANA has dried on the plant, there is no detectable residual product. Accordingly, 

the dietary risk assessment of ECANA suggests that the lack of exposure to residues of the 

extract obviate any dietary hazard, and it supports an exemption from the requirement to establish 

a tolerance.

Even if residues were found, they would not be cause for concern because ECANA has been fully

assessed and found not to be of toxicological concern. Humans regularly consume all the 

constituent components in the extract through consumption of fruits and vegetables. This regular 

dietary exposure has not resulted in any known incidents of toxic effect. Moreover, the three 

primary terpene constituents, comprising 70% of the active ingredient, have been approved by

FDA for use in cosmetics and as food additives. Finally, information submitted on the acute 

toxicity, developmental toxicity, and genotoxicity of ECANA confirm a very low toxicity profile. 

In sum, no dietary exposure is expected; but any potential dietary exposures would not be 

expected to pose any quantifiable risk due to a lack of residues of toxicological concern.
ECANA and ECANA Mimic Page 11 of 60

Biopesticides Registration Action Document

An assessment of ECANA’s three major terpene constituents- alpha-terpinene, p-cymene, and dlimonene - further substantiate ECANA’s low toxicity profile. All the constituents occur 

naturally in fruits, vegetables, herbs, spices, and other foods and beverages, and are defined as 

marker compounds in the blended active ingredient. These three compounds also are permitted as 

food and fragrance additives in the U.S. and Europe. Each of the constituents has been fully

characterized by EPA, and each has been reassessed for its use in pesticides for food uses in the 

October 2008 risk assessment referenced above. Based on the information before the Agency, 

incidental exposures to these three compounds are without known toxicological incident for 

humans. The general public is exposed daily to low levels of these compounds via ingestion, 

dermal contact, inhalation through consumption of foods and beverages, and dermal contact with 

cosmetics, in excess of any exposure expected to result from the use of this extract as a pesticide. 

The per capita daily consumption of these terpene compounds as food additives alone amounts to 

13.325 milligrams (mg) in the U.S. and 40.397 mg in Europe (WHO. Evaluation of Certain Food 

Additives. WHO Technical Report Series No. 928. Sixty-third Report of the Joint FAO/WHO 

Expert Committee on Food Additives. 2005), amounts far in excess of any potential dietary

exposures resulting from exposures to residues from this pesticidal extract, as discussed below. 

Alpha-terpinene is found in the essential oils of a variety of plants including citrus, peppermint, 

thyme, basil, and papaya. Per 21 CFR 172.515, it is permitted for direct addition to food for 

human consumption. D-limonene is a major component of lemon oil, orange oil, and grapefruit 

oil, and it is a minor component of other fruits, vegetables, meats, and spices. It is widely used as 

a flavor and fragrance, and it is generally recognized as safe (GRAS) by the Food and Drug 

Administration (FDA) as a food additive or flavoring, and as a fragrance additive (21 CFR 

182.60). Limonene is a registered by EPA as an active ingredient in 15 pesticides and is exempt

from the requirement of a tolerance per 40 CFR 180.539. Humans regularly consume p-cymene 

through such foods as butter, carrots, nutmeg, orange juice, oregano, raspberries, lemon oil, and 

spices. P-cymene is permitted by FDA for direct addition to food as a flavoring substance (21 

CFR 172.515). For the reasons set forth below, EPA concludes that the terpene exposures 

identified above will either exceed or be comparable to exposures resulting from the use of this 

extract as a pesticide. The balance of the constituents, while not expected to be active, is also 

regularly found in fruits, vegetables, and plant extracts, and determined by EPA not to be of 

toxicological concern when used in pesticides for food uses (Risk Assessments for Extract of 

Chenopodium ambrosioides near ambrosioides dated February 2008 and October 2008). Overall, 

a thorough analysis of the constituent compounds of Extract of Chenopodium ambrosioides near 

ambrosioides indicates a low toxicity profile and supports an exemption from the requirement of 

a tolerance.

ECANA and ECANA Mimic are applied directly to food commodities and degrade extremely

rapidly. However, the Agency notes that, in any event, no health risks are expected from 

pesticidal exposure to ECANA or ECANA Mimic. 

The Agency considered human exposure to ECANA in light of the relevant safety factors in FQPA 

and FIFRA. A determination has been made that no unreasonable adverse effects to the U.S. 

population in general, and to infants and children in particular, will result from the use of ECANA

when label instructions are followed. The Final Rule for ECANA, which provides a more in depth 
ECANA and ECANA Mimic Page 12 of 60

Biopesticides Registration Action Document

characterization of the lack of risk, can be found at: http://www.thefederalregister.com/d.p/2009-

01-07-E8-31408.

C. Environmental Assessment (40 CFR § 158.2060)

All nontarget organism, fate, and expression data requirements for ECANA and ECANA Mimic

have been met. They are summarized below in Table 8 of Appendix A.

1. Nontarget Organism Toxicology

The avian acute oral study (OPPTS 885.4050) and nontarget insects study (OPPTS 850.4350) 

show practically no toxicity to birds or nontarget insects. A waiver granted for the avian dietary

study (OPPTS 850.2200) makes clear the low probability of dietary exposure to birds. The 

freshwater fish study (OPPTS 850.1075) demonstrated moderate toxicity to fish, and an aquatic 

invertebrate study (OPPTS 850.1010) showed high toxicity to daphnids (water fleas). Limited use 

patterns and restrictive labeling language will preclude significant risk. A waiver was granted for 

nontarget plants (OPPTS 850.4350) primarily on the grounds that the applications of ECANA are 

directed and nontoxic to most plant species (MRID 47209703). Summary information supporting 

the nontarget organism data can be found in Table 8 of Appendix A. 

2. Environmental Fate and Ground Water Data 

Environmental fate and groundwater data for ECANA and ECANA Mimic were sufficient for 

registration. Physical chemistry data extracted from MRIDs 46396204-46396206, 46858802, 

47209701, 47209702 and data extracted from a nontarget fish study (47209706) and an aquatic 

invertebrate study (MRID 47209707) were used in EPA modeling to help quantify the 

environmental risks. The Agency estimated aquatic residues using the screening model GENeric 

Estimated Exposure Concentration - GENEEC v2.0. Based on the estimated environmental 

concentrations (EECs) from the GENEEC model (Table 9) and results from aquatic studies risk 

quotients (RQs) were derived to evaluate the potential risk to aquatic animals (Table 9). The 

calculated RQs were below the EPA levels of concern (LOC) for fish and aquatic invertebrates 

(including endangered species) chronically exposed to a pesticide when applied in accordance with 

the label directions. 

The GENEEC model uses environmental parameters that are typical for a “standard agricultural 

field-farm pond” to estimate aqueous residues. The primary assumption with GENEEC is that the 

fields receive one single large rainfall event (either immediately after application or after a latent 

period of 2 days) that washes a certain quantity of pesticides from the fields and into the pond. 

Degradation and adsorptive processes modify the initial mass of pesticides in both environments. 

Unlike with foliar residues, the constituent markers of ECANA are not degraded or volatilized 

quickly in water. (The EPA-estimated aqueous half-lives for all the constituent markers from a 

variety of residue measurements taken during the aquatic daphnid study (MRID 47209707), fish 

toxicity study (MRID 47209706), and aqueous stability study (MRID 47209707) ranged from 

35.87 hours to 866.67 hours.)
ECANA and ECANA Mimic Page 13 of 60

Biopesticides Registration Action Document

Aqueous ECANA residue concentrations were estimated by GENEEC for a wide variety of 

scenarios using aerial, groundboom, and airblast applications. Applications were either “watered 

in” with a rain event shortly following application or allowed to dry for 2 days prior to a rain event. 

Rain events immediately following application increased residue concentrations in the farm pond 

to levels such that Daphnid RQs exceeded the LOCs for every application method. Fish remained 

below the LOCs for normal and threatened and endangered species, suggesting low relative risk to 

fish. Daphnid RQs from groundboom and airblast to vineyards applications also were below the 

LOCs for normal and threatened and endangered species when residues were allowed to dry for 2 

days. As with fish RQs, this suggested that there is low relative risk to aquatic invertebrates from 

these application methods. The only aerial application to have Daphnid RQs less than the LOCs 

was one in which the spray dried for 2 days and the application had a 370 foot buffer. Similar traits 

were observed for airblast operations to orchards when using a 14-foot buffer.

LOC exceedences for certain application methods and environmental conditions required that 

restrictions or limitations be added to the proposed label to reduce the potential for “high acute 

risk” in aquatic invertebrates. Specifically, aerial, airblast, and groundboom outdoor methods of 

application (and presumably other more manual methods such as handwand, as well) require plant 

treatment prior to a rain event. Additionally waterbody spray buffers of 14 feet (groundboom and 

airblast) and 370 feet (aerial) are required to mitigate potential “high acute risk” to aquatic 

invertebrates.

3. Ecological Exposure and Risk Characterization

The proposed uses of ECANA and ECANA Mimic are not likely to result in any ecological risk. 

Applications of ECANA and ECANA Mimic are not expected to result in significant ecological 

exposure. The following factors are expected to address any exposure concerns: (1) Applications 

are terrestrial and targeted at commercial crops, which are not optimal foraging habitats for most 

avian, mammalian, and invertebrate species; (2) The foliar half-life of the extract is very short 

(estimated half-life 7.36 minutes) following application. The lack of persistence precludes the 

opportunity for significant incidental terrestrial exposures and makes it highly unlikely that 

residues may migrate to water in the event of a rain event, effectively precluding any exposures to 

aquatic nontarget organisms; (3) Potential spray drift is mitigated through labeling provisions for 

buffer requirements and applications intervals prior to rain events, further obviating both nontarget

terrestrial and aquatic exposures; and (4) Even in the event of terrestrial exposure, data suggest a 

lack of acute toxicity to terrestrial nontarget organisms. In short, a lack of exposure precludes all 

risk; and data suggest that there is no significant toxicity for terrestrial nontarget organisms for this 

pesticide, which is limited to terrestrial use. 

4. Endangered Species Statement

In March 2008, an ‘Environmental Risk Assessment’ on ECANA found that pesticidal use of the 

active ingredient would result in negligible ecological exposure and that ECANA was ‘Not Likely

to Adversely Affect’ (NLAA) threatened and endangered (T&E) species. At that time, an NLAA 
ECANA and ECANA Mimic Page 14 of 60

Biopesticides Registration Action Document

finding did not require consultation and concurrence with U.S. Fish and Wildlife Service

(USFWS). In March of 2010, an ‘Environmental Risk Assessment’ on ECANA Mimic determined 

that the NLAA finding for ECANA could be extended to ECANA Mimic as it is effectively the 

same compound. Five primary factors limit the potential for threatened and endangered species to 

be exposed: (1) Proposed use patterns are directed at commercial crops and preclude spraying near 

critical habitat for threatened and endangered species. (2) The rapid foliar 

degradation/volatilization (maximum estimated half-life 7.36 minutes) precludes endangered 

species from being exposed. (3) The proposed use areas are not optimal foraging habitat for most 

avian and mammalian species, thereby lessening the potential for species to move into these areas 

following ECANA applications. (4) The proposed buffers around water bodies will substantially

mitigate spray drift into water bodies, thereby reducing or eliminating exposures to T&E aquatic 

organisms. (5) The proposed restriction from application prior to rain events also will substantially

mitigate the potential for residues to drift or be washed into water bodies, reducing or eliminating 

exposures to T&E aquatic organisms.

D. Efficacy Data

No efficacy data were submitted in support of label claims and product performance for 

applications containing ECANA. While the conduct of product performance studies (Efficacy

Trials, OPPTS 810.300) is listed as a requirement for all pesticide products, the Agency

customarily only requires these data to be submitted for review in connection with the registration 

of products directly pertaining to the mitigation of disease bearing human health organisms and 

certain designated Quarantine Pests, i.e., ticks, mosquitoes, fleas, Mediterranean fruit flies, Gypsy

Moths, Japanese Beetles, etc. In this case, the registrant did not make any public health pest claims 

on the label, and a submission of efficacy data was not requested. Nonetheless, the registrant 

claims to have such data on file, and is prepared to submit if requested.

The registrant has made claims against plant-feeding mites. While an unqualified claim against 

mites or a species-specific claim against a public health pest mite would trigger an efficacy

submission, the registrant has submitted information showing that plant-feeding mites as a class 

are fundamentally different than any species of mite listed as a public health pest in Pesticide 

Registration Notice 2002-1.

V. RISK MANAGEMENT DECISION

A. Determination of Eligibility for Registration

Section 3(c)5) of FIFRA provides for the registration of new active ingredients if it is determined 

that: (A) its composition is such as to warrant the proposed claims for it; (B) its labeling and other 

materials required to be submitted comply with the requirements of FIFRA; (C) it will perform its 

intended function without unreasonable adverse effects on the environment; and (D) when used in 

accordance with widespread and commonly recognized practice, it will not generally cause 

unreasonable adverse effects on the environment. 
ECANA and ECANA Mimic Page 15 of 60

Biopesticides Registration Action Document

To satisfy criteria “A” above, ECANA products are not expected to cause unreasonable adverse 

effects when used according to label instructions. Criteria “B” is satisfied by the current label and 

the data presented in this document. The Agency believes that this pesticidal active ingredient 

will not cause any unreasonable adverse effects, as an insecticide and acaricide, and does provide 

protection as claimed satisfying Criteria “C.” Criteria “D” was satisfied by the data presented in 

the initial registration of this biochemical. Therefore, ECANA meets the standard for registration.

B. Regulatory Decision 

The data submitted fulfill the requirements of registration for use of ECANA as a biochemical 

insecticide and acaricide for use on food and ornamental crops. Refer to Appendix B for productspecific information.

On October 1, 2009, EPA announced a new policy to provide a more meaningful opportunity for 

the public to participate on major registration decisions before they occur. According to this new 

policy, EPA intends to provide a public comment period prior to making a registration decision 

for, at minimum, the following types of applications: new active ingredients; first food use; first 

outdoor use; first residential use; and actions for which the Agency anticipates substantial public 

interest. Accordingly, ECANA Mimic is subject to a 30-day comment period as a new active 

ingredient with both food uses and outdoor uses. 

At this time, EPA believes, the data submitted fulfill the requirements of registration for products 

containing ECANA Mimic as an insecticide and acaricide. Acute toxicity data for ECANA Mimic 

demonstrate that it is toxicity category III and IV for all routes of exposure. (No toxicological 

endpoints were established.) Data confirm that ECANA Mimic does not demonstrate subchronic 

toxicity, and it is not mutagenic or genotoxic. While a developmental toxicity endpoint was 

established for occupational workers, EPA concludes that a lack of exposure precludes any

potential risk. EPA has no concerns for any terrestrial nontarget organisms exposed to ECANA 

Mimic in accordance with its approved uses. EPA has not identified any toxic endpoints for 

nontarget mammals, birds, plants, or soil organisms; and while ECANA Mimic demonstrates 

some aquatic toxicity, no aquatic exposure is expected. Because of a lack of exposure and the 

virtual nontoxicity of ECANA Mimic to terrestrial nontarget organisms, EPA there are no 

concerns for any threatened and endangered species. Given, the nontoxic character of ECANA 

Mimic, EPA supports its registration under Section 3(c)(5) of the Federal Insecticide, Fungicide, 

and Rodenticide Act (FIFRA). Refer to Appendix B for product-specific information.

1. Conditional/Unconditional Registration

All data requirements are fulfilled, and EPA has determined that unconditional registration of 

ECANA is appropriate.

All data requirements are fulfilled and EPA has preliminarily determined that an unconditional 

registration for ECANA Mimic is warranted under Section 3(c)(5) of FIFRA.
ECANA and ECANA Mimic Page 16 of 60

Biopesticides Registration Action Document

C. Environmental Justice

EPA seeks to achieve environmental justice-- the fair treatment and meaningful involvement of all 

people, regardless of race, color, national origin, or income --in the development, implementation, 

and enforcement of environmental laws, regulations, and policies. At this time, EPA does not 

believe that use of pesticide products containing this active ingredient will cause harm or a 

disproportionate impact on at-risk communities.

For additional information regarding environmental justice issues, please visit EPA’s website at: 

http://www.epa.gov/compliance/environmentaljustice/index.html.

VI. Terms and Conditions of the Registrations

While EPA supports the unconditional registrations of ECANA and ECANA Mimic, 

applications of these active ingredients are qualified to provide further protection for human 

health and the environment. The qualifications follow:

1. ECANA and ECANA Mimic are commercial pesticides, and are presently not labeled for 

residential use. 

2. Aerial applications and chemigation (injecting a chemical into irrigation water and applying it 

through the system to the crop or field) are presently prohibited. 

3. ECANA and ECANA Mimic registrations presently require 15 foot spray buffers around 

water bodies

4. ECANA and ECANA Mimic registrations prohibit groundboom and airblast applications 

when winds exceed 10 mph.

5. ECANA and ECANA Mimic registrations prohibit application within 2 hours of an expected 

rain event.

 

6. ECANA and ECANA Mimic cannot be applied to a crop more than 10 times per year. 

7. ECANA and ECANA Mimic are prohibited for use on the following ornamentals: flowering 

Cyclamen, Gerbera, Begonia, and Impatiens. 
ECANA and ECANA Mimic Page 17 of 60

Biopesticides Registration Action Document

VI. Appendix A

TABLE 1. Product Chemistry for ECANA (40 CFR § 158.2030)

TABLE 1. Product Chemistry for ECANA

Guideline Reference 

No. /Property ECANA Citation

830.1550 Product 

Identity

Active Ingredient - 100.0% by weight (extract of 

Chenopodium ambrosioides var. ambrosioides). Impurities 

identified. ECANA Mimic does not have ECANA’s impurities. 

ACCEPTABLE

MRID 46396201

47209701 47209702

830.1620 

Manufacturing Process

ECANA is manufactured by a distillation process. ECANA 

Mimic is produced by blending terpenes. ACCEPTABLE

MRID 46396201

47209701 47209702

830.1670 Formation 

of Unintentional 

Ingredients

Impurities present in the product have been described 

quantitatively. Ascaridole has been eliminated in the ECANA 

product. ECANA Mimic does not carry the same degree of 

impurities. ACCEPTABLE

MRID 46396201

47209701 47209702

830.1700 Analysis of 

Samples

The three main constituents of ECANA are analyzed. A 

number of individual samples are outside proposed certified 

limits. Blending brings batches within limits. ECANA Mimic 

is within Agency recommended limits. ACCEPTABLE

MRID 46396202

830.1750 

Certification of Limits

Considering the variability of plant extracts, the certified limits 

of ECANA are ACCEPTABLE

MRID 46396202

47209701 47209702

830.1800 Analytical 

Methods

The enforcement analytical method is GC-MS for the three 

central constituents of the active ingredient. Samples are 

diluted into acetonitrile, analyzed, and quantified against 

known standards on a Varian 3900 GC system with a Saturn 

2100T ion trap mass selective detector. ACCEPTABLE

MRID 46396202

47209701 47209702

TABLE 2. Physical and Chemical Properties for ECANA (40 CFR § 158.2030)

TABLE 2. Physical and Chemical Properties for ECANA ( MRIDs 46396204-46396206, 46858802, 

47209701, 47209702)

Guideline Reference No. /Property ECANA

830.6302 Color Pale amber, light brown. Color code 136. Color composition is 

red=252, green=191, blue=73.

830.6303 Physical State Semi-volatile liquid

830.6304 Odor Fruity, woodsy, aromatic, characteristic of monoterpene blend.

830.6313 Stability Stability during 14-day exposure to iron chips, aluminum wire pieces, 

iron citrate, or aluminum acetate @ room temperature or 54C

830.6314 Oxidation/Reduction: 

Chemical Incompatibility Does not contain oxidizers or reducing agents

830.6315 Flammability Flash point = 118°F

830.6316 Explodability Non-explosive

830.6317 Storage Stability Submitted and acceptable

830.6319 Miscibility Not applicable, not intended to be diluted with oil or non-polar 

solvents

830.6320 Corrosion Characteristics Submitted and acceptable
ECANA and ECANA Mimic Page 18 of 60

Biopesticides Registration Action Document

830.6321 Dielectric Breakdown 

Voltage

Not required for MP or TGAI

830.7000 pH 5.30-5.68, mean of 5.46±0.20 (1% dispersion in deionized water)

830.7050 UV/Visible Not applicable

830.7100 Viscosity 1.79 mPa.s. (Std Dev = 0.16)

830.7200 Melting Range Not applicable

830.7220 Boiling Range 170-188C

830.7300 Bulk Density 0.875 - 0.914, mean of 0.895±0.020 g/mL

830.7370 Dissociation Constant in 

Water Not applicable

830.7520 Particle Size/Distribution Not applicable

830.7550 Partition Coefficient

Marker# 1 = 5.09

Marker# 2 = 5.08

Marker# 3 = 4.85

830.7840 Water Solubility

Marker# 1 = 390-511 mg/L, mean of 436±54 mg/L

Marker# 2 = 138-188 mg/L, mean of 160±21 mg/L

Marker# 3 = 145-212 mg/L, mean of 183±25 mg/L

830.7950 Vapor Pressure 2613 Pa (0.38 psig) @ 20C

3157 Pa (0.46 psig)@ 25C

Table 3: Physicochemical Properties of α-Terpinene (40 CFR § 158.2030)

Parameter OPPTS # Value (active ingredient)

Color 830.6302 Clear, nearly colorless

Physical State 830.6303 Liquid

Odor 830.6304 Citrus

Stability 830.6313 Refer to ECANA Mimic data

Flammability 830.6315 117 °F (Tag Closed Cup)

Storage Stability 830.6317 Not Applicable

Miscibility 830.6319 Not Applicable

Corrosion Characteristics 830.6320 Not Applicable

Melting point 830.7200 Not Applicable

Boiling point 830.7220 173 - 175° C

pH 830.7000 Not Applicable

Density (20° C) 830.7300 0.840 g/cm3

Water solubility (25° C) 830.7840 5.6 mg/L

Solvent solubility (20° C) Soluble in alcohol (100 wt%)

Vapor pressure at 20° C 830.7950 0.8 mm Hg
ECANA and ECANA Mimic Page 19 of 60

Biopesticides Registration Action Document

Octanol/water partition 

coefficient, log (Kow)

830.7570 5.09

UV/visible absorbance 

maxima 830.7050 265 nm

Viscosity 830.7100 Not Determined – like water

Log Koc (Kow method) 3.688

Koc (Kow method) 4877 L/kg

Henry’s Law Constant 5.250 E-2 atm-m3/mole

Surface Tension (dyne/cm) 26.8 ± 3.0

Table 4: Physicochemical Properties of d-limonene (40 CFR § 158.2030)

Parameter OPPTS # Value (active ingredient)

Color 830.6302 Clear, nearly colorless

Physical State 830.6303 Liquid

Odor 830.6304 Citrus

Stability 830.6313 Refer to ECANA Mimic data

Flammability 830.6315 122 °F (Tag Closed Cup)

Storage Stability 830.6317 Not Applicable

Miscibility 830.6319 Not Applicable

Corrosion Characteristics 830.6320 Not Applicable

Melting point 830.7200 Not Applicable

Boiling Point 830.7220 178° C

pH 830.7000 Not Applicable

Density (20° C) 830.7300 0.841 g/cm3

Water solubility (25° C) 830.7840 14 mg/L

Solvent solubility Soluble in alcohol (100 wt%)

Vapor pressure at 20° C 830.7950 1.0 mm Hg

Octanol/water partition 

coefficient, log (Kow)

830.7570 4.85

UV/visible absorbance maxima 830.7050 No absorbance 190 – 800 nm

Viscosity 830.7100 Not Determined – like water

Log Koc (Kow method) 3.801

Koc (Kow method) 6324 L/kg

Henry’s Law Constant 2.535 E-2 atm-m3/mole

Surface Tension (dyne/cm) 25.8 ± 3.0
ECANA and ECANA Mimic Page 20 of 60

Biopesticides Registration Action Document

Table 5: Physicochemical Properties of p-cymene (40 CFR § 158.2030)

Parameter OPPTS # Value (active ingredient)

Color 830.6302 Clear, nearly colorless

Physical State 830.6303 Liquid

Odor 830.6304 Lemon

Stability 830.6313 Refer to ECANA Mimic data

Flammability 830.6315 125 °F (Tag Closed Cup)

Storage Stability 830.6317 Not Applicable

Miscibility 830.6319 Not Applicable

Corrosion Characteristics 830.6320 Not Applicable

Melting point 830.7200 Not Applicable

Boiling point 830.7220 177° C

pH 830.7000 NA

Density (20° C) 830.7300 0.855 g/cm3

Water solubility (25° C) 830.7840 23 mg/L

Solvent solubility Soluble in alcohol (100 wt%)

Vapor pressure at 25° C 830.7950 1.46 - 2.0 mm Hg

Octanol/water partition 

coefficient, log (Kow)

830.7570 5.08

UV/visible absorbance maxima 830.7050 198 nm

Viscosity 830.7100 Not Determined – like water

Log Koc (Kow method) 3.558

Koc (Kow method) 3614 L/kg

Henry’s Law Constant 1.121 E-2 atm-m3/mole

Surface Tension (dyne/cm) 28.5 ± 3.0

TABLE 6. Toxicology Data for ECANA (40 CFR § 158.2050)

TABLE 6. Toxicology Data for ECANA

Guideline Reference 

No. /Property

Description of Result Methods

870.1100 Acute 

Oral Toxicity

Oral LD50 between 2000 and 5000mg/kg, Toxicity Category III; 

ACCEPTABLE

MRID 46396207

870.1200 Acute 

Dermal Toxicity

Dermal LD50>5000mg/kg, Toxicity Category IV; ACCEPTABLE MRID 46396208
ECANA and ECANA Mimic Page 21 of 60

Biopesticides Registration Action Document

870.1300 Acute 

Inhalation Toxicity

Inhalation LC50>2.03mg/L, Toxicity Category IV; ACCEPTABLE MRID 46396209

870.2400 Primary 

Eye Irritation

No corneal opacity or iritis noted. Conjunctival irritation resolving by day 4, 

Toxicity Category III; ACCEPTABLE

MRID 46396210

870.2500 Primary 

Dermal Irritation

Very slight erythema persisting through day 14. Very slight edema clearing 

by day 7, Toxicity Category IV; ACCEPTABLE

MRID 46396211

870.2600 

Hypersensitivity

Sensitizer; ACCEPTABLE MRID 46396212

Hypersensitivity 

Incidents

Incidents must be reported Incidents must be 

reported

870.5000 

Genotoxicity

Not mutagenic to bacterial strains TA98, TA100, TA1535, TA1537 and E 

coli strain WP2 uvrA W, ACCEPTABLE

No chromosome aberrations in human lymphocytes, ACCEPTABLE

No induction of DNA repair response in rat hepatocytes, ACCEPTABLE

MRID 46456301

MRID 46396214

MRID 46396215

880.3550 Immune 

Response

ACCEPTABLE limited exposure waiver rationale MRID 47209703

870.3100 90-day 

Feeding (1 spp)

ACCEPTABLE limited exposure waiver rationale MRID 47209703

870.3250 90-day 

Dermal (1 spp)

ACCEPTABLE limited exposure waiver rationale MRID 47209703

870.3465 90-day 

Inhalation (1 spp)

ACCEPTABLE limited exposure waiver rationale MRID 47209703

870.3700 Prenatal 

Development (1 spp)

ACCEPTABLE limited exposure waiver rationale; also Toxicity 

endpoint based on Marker study in rats:

Maternal NOAEL=60 mg/kg-day

LOAEL=125 mg/kg-day; based on decreased whole body weight and 

body weight minus uterine weights during pregnancy

Developmental NOAEL=30 mg/kg-day

LOAEL=60 mg/kg-day based on abnormal ossification, increases in 

skeletal abnormalities and kidney weights

MRID 47209703

Table 7. ECANA Mimic Acute Toxicity Results (40 CFR § 158.2050)

Study Type/OPPTS Guideline LD50/LC50/Results Toxicity 

Category

MRID

Acute Inhalation Toxicity/OPPTS 870.1300 > 5.14 mg/L IV 48064501

Acute Eye Irritation/OPPTS 870.2400 Minimally irritating IV 48064502

Acute Dermal Irritation/OPPTS 870.2500 Non-irritating IV 48064503

Skin Sensitization/OPPTS 870.2600 Not a sensitizer N/A 48064504

Table 8. Nontarget Toxicity of QRD 406 or its Marker Components (40 CFR § 158.2060)

Table 8. Nontarget Toxicity of QRD 406 or its Marker Components

Study Type/OPPTS 

Guideline

LD50/LC50/Results Toxicity 

Category

MRID
ECANA and ECANA Mimic Page 22 of 60

Biopesticides Registration Action Document

Avian Acute Oral

Colinus virginianus

(OPPTS 885.4050)

Acute oral LD50 for northern bobwhites >2250 mg/kg. 

ACCEPTABLE.

Practically 

Nontoxic 47209704

Avian Dietary

(OPPTS 850.2200) 

Waiver rationale based on low probability of exposure, 

low acute avian toxicity. ACCEPTABLE.

N/A 47209703

Freshwater Fish LC50

Pimephales promelas

(OPPTS 850.1075) 

96 hr Nominal conc; 20 mg/L

96 hr Modified for aqueous degradation; 2.2 mg/L (C.I. 

1.65-3.3) ACCEPTABLE.

Moderately 

Toxic

47209706

Freshwater Invertebrate

Daphnia magna

(OPPTS 850.1010) 

24, 48 hr Nominal conc; 3.3, 1.3 mg/L

24, 48 hr Modified for aqueous degradation; 0.594, 0.234 

mg/L (C.I. 0.414-0.864 and 0.164-0.306). 

ACCEPTABLE.

Highly 

Toxic 47209705

Nontarget Plants 

(OPPTS 850.4100) 

Salvia, Cosmos, Sweet Allysum NOEC 1%, LOEC based 

on increased height at 2% and 4%.

Begonia NOEC, not determined; LOEC 2% based on 

foliar burn and flower discoloration at 14-21 days.

Vinca (strong varietal response) NOEC, not determined; 

LOEC 2% based on dose-dependent increase in foliar 

black spots.

Impatiens, Petunias NOEC, not determined; LOEC 2% 

based on dose-dependent decrease in the number of 

flowers and open flowers at 21 days

Marigold, Tomato, Zinnias NOEC 2%

Fury, Ingrid, Grandina Gerbera NOEC, not determined; 

LOEC 2% based on increase in mature flower necrosis at 

7 days, 4% based on increase in bud necrosis at 7 days

Jack Frost, Sabrina, Sonia Rose NOEC, not determined; 

LOEC 4% based on various endpoints such as increased 

film, increased necrosis on new and old growth foliage by 

7 days, increased necrosis on mature flowers and flower 

buds by 7 days. ACCEPTABLE.

N/A 47209703

Nontarget Insects 

Orius insidiosus

Aphidius colemani

Bombus impatiens

(OPPTS 850.4350)

Orius insidiosus; 48 h; contact toxicity; nymph 29.15 g/L 

(C.I. 23.33-36.14), adult 11.28 g/L (C.I. 7.32-15.31)

Aphidius colemani; 48 h; contact toxicity; females 13.23 

g/L (C.I. 7.50-18.90), males 7.70 g/L (C.I. 1.57-16.57)

Bombus impatiens; 7, 14 day; contact toxicity; 0.1458 

mg/cm2(C.I. 0.1180-0.2043), 0.0771 mg/cm2(C.I. 

0.0475-0.1723)

Bombus impatiens; 7, 14 day; contact toxicity; 0.1076 

mg/bee, 0.0569 mg/bee; 7, 14 day LC50 (95% C.I.); 

15.4% (7.68-101.19), 8.75% (5.69-17.67) 

ACCEPTABLE.

Green peach aphid; 24 h; 0.63% (C.I. 0.47-0.79%)

Western flower thrip; 24 h; 0.0034 mg/cm2(C.I. 0.0027-

0.0039 mg/cm2) 

Greenhouse whitefly; 24 h; 0.0066 mg/cm2(C.I. 0.0054-

0.0076 mg/cm2) ACCEPTABLE.

III

Practically

Nontoxic

47209703
ECANA and ECANA Mimic Page 23 of 60

Biopesticides Registration Action Document

Table 9. Aqueous Concentrations of ECANA Modeled from GENEEC

1 Daphnid RQs = Peak EEC concentration (mg/L)/0.234 mg/L

2

Fish RQs = Maximum 4 day Average EEC concentration (mg/L)/2.2 mg/L

Table 9. Aqueous Concentrations of ECANA Modeled from GENEEC Following Various Outdoor 

Application Methods

Application 

Method Spray 

Buffe

r (ft)

Wet 

In 

from 

Rain 

Event

Peak EEC 

Concentratio

n

(μg/L)

1

Daphnid 

RQ

2

Fish 

RQ

Max 4 

Day 

Averag

e 

(μg/L)

Max 

21 Day 

Averag

e 

(μg/L)

Max 

60 Day 

Averag

e 

(μg/L)

Max 90 

Day 

Average 

(μg/L)

Airblast - orchards 100 

(14) No 3.92 0.02 

(0.05)

<0.0

1

3.69 2.09 0.88 0.59

Airblast - vineyards Non

e

No 2.67 0.01 <0.0

1

2.52 1.42 0.60 0.40

Airblast - vineyards

100 No 0.382 <0.01 <0.0

1

0.359 0.204 0.086 0.058

Groundboom - high 

boom - fine spray 100 No 2.13 0.01 <0.0

1

2.00 1.13 0.48 0.32

Groundboom - low 

boom - fine spray

Non

e

No 4.95 0.02 <0.0

1

4.67 2.64 1.12 0.75

Groundboom - low 

boom - fine spray 100 No 0.863 <0.01 <0.0

1

0.813 0.460 0.194 0.130
ECANA and ECANA Mimic Page 24 of 60

Biopesticides Registration Action Document

VII. Appendix B

REFERENCES

Araujo, I.B., Souza, C.A.M., De-Carvalho, R.R., Kuriyama, S.N., Rodrigues, R.P., Vollmer, R.S., 

Alves, E.N., and F.J.R. Paumgartten. 1996. Study of the embryofoetotoxicity of α-terpinene in the rat. 

Food and Chemical Toxicology. 34: 477-482.

El-Mofty, M.M., Khudoley, V.V., Sakr, S.A., and N.F. Ganem. 1992. Induction of neoplasms in 

Egyptian toads Bufo regularis by oil of Chenopodium. Oncology. 49: 253-255.

EPA. 1994. Reregistration eligibility document (RED): Limonene. EPA 738-R-94-034.

EPA. 1997. Standard operating procedures (SOPs) for residential exposure assessments. Contract 

No. 68-W6-0030.

EPA. 1998. PHED surrogate exposure guide. Estimates of worker exposure from the Pesticide 

Handler Exposure Database version 1.1. Scenario 32, 34-36.

Kapadia, G.J., Chung, E.G., Ghosh, B., Shukla, Y.N., Basak, S.P., Morton, J.F., and S.N. 

Pradhan. 1978. Carcinogenicity of some folk medicinal herbs in rats. J. Natl Cancer Inst. 60: 683-686. 

NPIC. 2006. Biopesticide incidents: Reporting period April 1, 1995 to August 21, 2006. National 

Pesticide Information Center. Environmental and Molecular Toxicology, Oregon State University. 

Report Number EPAKCa09/12/2006. 6pp.

World Health Organization. 2005. Evaluation of certain food additives. WHO Technical Report 

Series 928. 63rd report of the Joint FAO/WHO Expert Com. on Food Additives. 157 pp.
ECANA and ECANA Mimic Page 25 of 60

Biopesticides Registration Action Document

VIII. Appendix C

Product Labels

A. ECANA Manufacturing Use Product Label

B. ECANA End Use Product Label

C. ECANA Mimic Manufacturing Use Product Label

D. ECANA Mimic End Use Product Label
ECANA and ECANA Mimic Page 26 of 60

Biopesticides Registration Action Document

A. Manufacturing Use Product

QRD 406

FOR FORMULATION INTO INSECTICIDE AND ACARICIDE PRODUCTS

FOR MANUFACTURING OR FORMULATION USE ONLY

Active Ingredient:

Extract of Chenopodium ambrosioides near ambrosioides.................................................100.0%

Total: 100.0%

KEEP OUT OF REACH OF CHILDREN

CAUTION

FIRST AID

If In Eyes

 Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove 

contact lenses, if present, after the first 5 minutes, then continue rinsing eye.

 Call a poison control center or doctor for further treatment advice.

If Swallowed

 Call poison control center or doctor for treatment advice.

 Have person sip a glass of water if able to swallow.

 Do not induce vomiting unless told to do so by the poison control center or doctor.

 Do not give anything by mouth to an unconscious person.

If on Skin or 

Clothing:

 Take off contaminated clothing. 

 Rinse skin immediately with plenty of water for 15-20 minutes. 

 Call a poison control center or doctor for treatment advice. 

HOT LINE NUMBER

CHEMTREC (800) 424-9300 (24 hours)

Have the product container or label with you when calling a poison control center or doctor or going for 

treatment.

See side/back panels for additional precautionary statements

EPA Reg No. 69592-21 EPA Establishment No. 69592-MEX-1

Net Contents: 2.5 gallon (320 fl. oz.) (9.5 L)

 [4 gallon (15.2 L)]

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150
PRECAUTIONARY STATEMENTS

HAZARD TO HUMANS AND DOMESTIC ANIMALS

CAUTION: Harmful if swallowed. Causes moderate eye irritation. Avoid contact with eyes or 

clothing. Wash thoroughly with soap and water after handling and before eating, drinking, 

chewing gum or using tobacco. Prolonged or frequently repeated skin contact may cause 

allergic reactions in some individuals. Mixers, loaders and handlers must wear the appropriate 

personal protective equipment (PPE): coveralls, chemical-resistant gloves and protective 

eyewear. Remove and wash contaminated clothing before reuse.

ENVIRONMENTAL HAZARDS 

Do not discharge effluent containing this product into lakes, streams, ponds, estuaries, oceans or 

other waters unless in accordance with the requirements of a National Pollutant Discharge 

Elimination System (NPDES) permit and the permitting authority has been notified in writing prior 

to discharge. Do not discharge effluent containing this product to sewer systems without previously

notifying the local sewage treatment plant authority. For guidance contact your State Water Board 

or Regional Office of the EPA. Do not contaminate water when disposing of equipment 

washwaters or rinsate. 

This pesticide is toxic to aquatic invertebrates.

PHYSICAL OR CHEMICAL HAZARDS

Combustible. Do not use or store near heat or open flame.

DIRECTIONS FOR USE

It is a violation of Federal law to use this product in a manner inconsistent with its labeling.

QRD 406 is intended for use in the formulation of insecticides and acaricides to be applied to 

field and container-grown non-food ornamental plants in commercial nurseries, greenhouses, 

and lath- and shade houses. 

This product may be used to formulate products for any additional uses not listed on the MP 

label if the formulator, user group, or grower has complied with U.S. EPA data submission 

requirements regarding the support of such uses. Products formulated from this technical 

material will require registration with the U.S. Environmental Protection Agency (EPA).

STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

Pesticide Storage: Store in a cool dry place. Avoid freezing. 

Pesticide Disposal: To avoid wastes, use all material in this container by application according to label 

directions. If wastes cannot be avoided, offer remaining product to a waste disposal facility or pesticide 

disposal program (often such programs are run by state or local governments or by industry).

Do not contaminate water when disposing of equipment washwater or rinsate. Pesticide wastes may be toxic. 

Improper disposal of unused pesticide, washwater or rinse water is a violation of federal law. 

Container Disposal: Nonrefillable container. Do not reuse or refill this container.

Triple rinse container (or equivalent) promptly after emptying. Triple rinse as follows: Empty the remaining 

contents into application equipment or a mix tank and drain for 10 seconds after the flow begins to drip. Fill 

the container 1⁄4 full with water and recap. Shake for 10 seconds. Pour rinsate into application equipment or a 

mix tank or store rinsate for later use or disposal. Drain for 10 seconds after the flow begins to drip. Repeat 

this procedure two more times. Then offer for recycling if available, or puncture and dispose of in a sanitary

landfill or by incineration. Do not burn unless allowed by state and local ordinances.
ECANA and ECANA Mimic Page 28 of 60

Biopesticides Registration Action Document

B. End Use Product Label

QRD 400

Insecticide and Acaricide

FOR USE ON NON-FOOD ORNAMENTALS (TREES, SHRUBS, AND BEDDING PLANTS)

NOT FOR RESIDENTIAL USE

Active Ingredient:

Extract of Chenopodium ambrosioides near ambrosioides..................................................................................25.0%

Other Ingredients .................................................................................................................................................75.0%

Total: 100.0%

KEEP OUT OF REACH OF CHILDREN

CAUTION

FIRST AID

If In Eyes

 Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove 

contact lenses, if present, after the first 5 minutes, then continue rinsing eye.

 Call a poison control center or doctor for further treatment advice.

In On Skin Or Clothing

 Take off contaminated clothing.

 Rinse skin immediately with plenty of water for 15-20 minutes.

 Call a poison control center or doctor for further treatment advice.

HOT LINE NUMBER 

CHEMTREC (800) 424-9300 (24 hours)

Have the product container or label with you when calling a poison control center or doctor or going for treatment.

See side/back panels for additional precautionary statements

EPA Reg No. 69592-22 EPA Establishment No. 69592-MEX-1

Net Contents: 1 gallon (128 fl. oz.) (3.8 L)

 [ 2.5 gallon (320 fl. oz.) (9.5 L)]

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150

PRECAUTIONARY STATEMENTS

HAZARD TO HUMANS AND DOMESTIC ANIMALS

CAUTION: Causes moderate eye irritation. Avoid contact with skin, eyes or clothing. Wash 

thoroughly with soap and water after handling and before eating, drinking, chewing gum or 
ECANA and ECANA Mimic Page 29 of 60

Biopesticides Registration Action Document

using tobacco. Remove and wash contaminated clothing before reuse. Prolonged or frequently

repeated skin contact may cause allergic reactions in some individuals. Wear the appropriate 

Personal Protective Equipment (PPE).

PERSONAL PROTECTIVE EQUIPMENT

Applicators mixers, loaders and other handlers must wear:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material

 Shoes plus socks

 Protective eyewear

 Coveralls for high-pressure handwand and groundboom applicators.

 For greenhouse uses, applicators and other handlers must wear a NIOSH-approved 

respirator with an organic vapor (OV) cartridge or canister with any R, P or HE filter.

Follow the manufacturer’s instructions for cleaning/maintaining PPE. If no such instructions for 

washables, use detergent and hot water. Keep and wash PPE separately from other laundry.

USER SAFETY RECOMMENDATIONS

Users should: 

● Remove clothing immediately if pesticide gets inside. Then wash thoroughly and put on clean 

clothing. 

● Remove PPE immediately after handling this product. Wash the outside of gloves before 

removing. As soon as possible, wash thoroughly and change into clean clothing.

ENVIRONMENTAL HAZARDS 

This product is toxic to aquatic invertebrates. For terrestrial uses: Do not apply directly to 

water or to areas where surface water is present, or to intertidal areas below the mean high water 

mark. Do not contaminate water when disposing of equipment washwater or rinsate. 

Drift and runoff may be hazardous to aquatic organisms in water adjacent to treated areas. Do 

not apply when weather conditions favor drift or runoff from treated areas. Do not apply when a 

rain event is expected within 2 hours. Air blast applications must maintain a spray buffer of 15 

feet from any waterbody. See the Directions for Use for details.

 

PHYSICAL OR CHEMICAL HAZARDS

Combustible. Do not use or store near heat or open flame. 

DIRECTIONS FOR USE

It is a violation of Federal law to use this product in a manner inconsistent with its labeling.

READ THE ENTIRE LABEL. USE STRICTLY IN ACCORDANCE WITH THE LABEL. 

FAILURE TO FOLLOW DIRECTIONS AND PRECAUTIONS ON THIS LABEL MAY 

RESULT IN CROP INJURY, POOR INSECT CONTROL, AND/OR ILLEGAL RESIDUES. 

Do not apply this product in a way that will contact workers or other persons, either directly or 

through drift. Only protected handlers may be in the area during application. For any requirements 

specific to your state or tribe, consult the State or Tribal agency responsible for pesticide regulation.
ECANA and ECANA Mimic Page 30 of 60

Biopesticides Registration Action Document

AGRICULTURAL USE REQUIREMENTS

Use this product only in accordance with its labeling and with the Worker Protection Standard, 40 

CFR Part 170. This Standard contains requirements for the protection of agricultural workers on 

farms, forests, nurseries, greenhouses and handlers of agricultural insecticides. It contains 

requirements for training, decontamination, notification and emergency assistance. It also contains 

specific instructions and exceptions pertaining to the statements on this label about personal 

protective equipment (PPE) and restricted-entry interval. The requirements in this box only apply to 

uses of this product that are covered by the Worker Protection Standard.

Do not enter or allow worker entry into treated areas during the restricted entry interval

(REI) of 4 hours.

PPE required for early entry to treated areas that is permitted under the Worker Protection Standard 

and that involves contact with anything that has been treated, such as plants, soil or water is:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material

 Shoes plus socks 

NON-AGRICULTURAL USE REQUIREMENTS

The requirements in this box apply to uses that are NOT within the scope of the Worker 

Protection Standard for agricultural pesticides (40 CFR Part 170). The WPS applies when this 

product is used to produce agricultural plants on farms, nurseries or green houses. 

Do not enter treated areas without protective clothing until spray has dried. Refer to 

Personal Protective Equipment section under the Precautionary Statements section.

GENERAL INFORMATION

QRD 400 is an Emulsifiable Concentrate (EC) Insecticide and Acaricide for Use on Field and 

Container-grown Non-Food Ornamental Plants in Commercial Nurseries, Greenhouses, and 

Lath- and Shade-houses. Not For Residential Use.

QRD 400 is a contact insecticide/acaricide for use in the control of aphids, plant-feeding mites, 

whiteflies, mealy bugs, leafminers, thrips, fungus gnats, and tarnished plant bugs. QRD 400 can 

be applied to field and container-grown non-food ornamentals – trees, Christmas trees, shrubs, 

flowers, annual and perennial bedding plants, potted flowers, and tropical plants.

MIXING AND APPLICATION INSTRUCTIONS

Do not apply this product by aerial application or through any type of irrigation system. 

QRD 400 is toxic to aquatic invertebrates. Users must maintain the following buffer zones: 

Airblast spray applicators must not apply within 15 feet of aquatic habitats (such as, but not 

limited to, lakes, reservoirs, rivers, streams, marshes, ponds, estuaries, and commercial fish 

ponds). Groundboom and airblast applicators must also account for wind direction and speed. 

Only apply this product if the wind direction favors on-target deposition. Do not apply when the 

wind velocity exceeds 10 mph. Do not apply when a rain event is expected within 2 hours. 
ECANA and ECANA Mimic Page 31 of 60

Biopesticides Registration Action Document

Shake container well before use. QRD 400 must be diluted with water for spray applications. 

Partially fill the spray tank with clean water, add the specified amount of QRD 400 to the tank 

(see table below), and finish filling the tank to the desired volume to obtain the proper spray

concentration for the desired application rate per acre.

Gallons Water 

per Acre

QRD 400 gallons

0.5% solution

QRD 400 gallons

1% solution

25 0.12 (16 fl oz) 0.25 (32 fl oz)

50 0.25 (32 fl oz) 0.5 (64 fl oz)

75 0.37 (48 fl oz) 0.75 (96 fl oz)

100 0.5 1

 For smaller quantities, mix at a rate of 0.65 – 1.3 fl. oz. QRD 

400 per 1 gallon of water.

Apply on a 7- to 14-day schedule as necessary. Apply QRD 400 at first sign of insect pressure 

or plant-feeding mite infestation. Use higher application rate under conditions of heavy pest 

pressure. Uniform coverage of both upper and lower leaves is critical for effective pest control. 

Avoid over spraying to the point of excessive runoff. Do not apply QRD 400 more than 10 

times per year. 

QRD 400 can be applied with commonly used ground equipment, hose-end, pressurized, 

greenhouse, and hand-held sprayers. 

Biological Control Agents: This product is compatible with the following biological control 

agents: Encarsia formosa, Amblyseius fallacis, Phytoseiulus persimilis, Orius insidiosus, 

Aphidius colemani, Zetzellia mali, Neoseiulus fallacies, Typhlodromus pyri, Stethorus punctum, 

lacewings, syrphid fly larvae, phytoseiid mite predators, aphid midges, lady beetles, and other 

predacious mites. Wait at least 2 hours after application before introducing new biological 

agents, or spray the evening before the day of introduction of new biological control agents. 

AgraQuest is committed to the practice of sound resistance management programs that include 

rotation and tank mixing with other treatments having a variety of modes of actions. 

Compatibility: Do not combine QRD 400 in the spray tank with other pesticides, surfactants, 

adjuvants, or fertilizers if there has been no previous experience or use of the combination to 

show it is physically compatible, effective and non-injurious under your use conditions. 

To ensure compatibility of tank-mix combinations they must be evaluated prior to use. To 

determine the physical compatibility of this product with other products, use a jar test. Using a

quart jar, add the proportionate amounts of the products to approximately one quart of water 

with agitation. Add dry formulations first, then flowables, then emulsifiable concentrates last. 

After thorough mixing, allow this mixture to stand for 5 minutes. If the combination remains 

mixed or can be readily remixed, it is physically compatible. Once compatibility has been 

proven, use the same procedure for adding required ingredients to the spray tank. Test the 

combination on a small number of plants to check for phytotoxicity before treating large areas. 

Do not use the combination if adverse effects are observed. 
ECANA and ECANA Mimic Page 32 of 60

Biopesticides Registration Action Document

PLANTS EVALUATED FOR PHYTOTOXICITY

QRD 400 has been evaluated alone in a wide range of crops and ornamentals. However, since 

testing on all varieties of all ornamentals is not feasible, testing a small portion of the area to be 

treated for phytotoxicity is required before treating the entire area. Further, all possible 

combinations or sequences of pesticide sprays, including other fertilizers, surfactants, adjuvants 

and other pesticides, have not been tested; thus, testing for compatibility and phytotoxicity of 

spray mixtures is required. Use the product in accordance with all label use directions. It is 

further required that spray equipment used to apply QRD 400 be thoroughly cleaned before use. 

Phytotoxicity has occasionally been observed following the use of this product on flowers of 

Cyclamen, Gerbera, Begonia, and Impatiens. Do not use QRD 400 on flowering Cyclamen, 

Gerbera, Begonia, and Impatiens. 

STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

Pesticide Storage: Store in a cool dry place. Avoid freezing. 

Pesticide Disposal: To avoid wastes, use all material in this container by application according to label directions. If 

wastes cannot be avoided, offer remaining product to a waste disposal facility or pesticide disposal program (often such 

programs are run by state or local governments or by industry).

Do not contaminate water when disposing of equipment washwater or rinsate. Pesticide wastes may be toxic. Improper 

disposal of unused pesticide, washwater or rinse water is a violation of federal law. 

Container Disposal: Non-refillable container. Do not reuse or refill this container

Triple rinse container (or equivalent) promptly after emptying. Triple rinse as follows: Empty the remaining contents 

into application equipment or a mix tank and drain for 10 seconds after the flow begins to drip. Fill the container 1⁄4 full 

with water and recap. Shake for 10 seconds. Pour rinsate into application equipment or a mix tank or store rinsate for 

later use or disposal. Drain for 10 seconds after the flow begins to drip. Repeat this procedure two more times. Then 

offer for recycling if available, or puncture and dispose of in a sanitary landfill or by incineration. Do not burn unless 

allowed by state and local ordinances.

WARRANTY AND DISCLAIMER STATEMENT

AgraQuest warrants to those persons lawfully purchasing this product that at the time of the first 

sale of this product by Seller that this product conformed to its description and was reasonably

fit for the purposes stated on the label when used in accordance with Seller’s directions. Buyers 

and users of this product assume the risk of any use contrary to such directions. EXCEPT AS 

PROVIDED ELSEWHERE IN WRITING CONTAINING AN EXPRESS REFERENCE TO 

THIS WARRANTY AND LIMITATION OF DAMAGES, SELLER MAKES NO OTHER 

EXPRESS OR IMPLIED WARRANTY OR GUARANTY, INCLUDING ANY OTHER 

EXPRESS OR IMPLIED WARRANTY OF FITNESS OR OF MERCHANTABILITY AND 

NO AGENT OF SELLER IS AUTHORIZED TO DO SO. Except to the extent prohibited by

applicable law, AgraQuest offers this product with the following conditions: 1) buyers and users 

of this product assume the risk of any storage, handling or use contrary to AgraQuest’s label and 

directions and 2) AgraQuest’s liability shall in no case exceed the purchase price of the 

applicable AgraQuest product.
ECANA and ECANA Mimic Page 33 of 60

Biopesticides Registration Action Document

C. ECANA Mimic Manufacturing Use Product Label

QRD 420

FOR FORMULATION INTO INSECTICIDE AND ACARICIDE PRODUCTS

FOR MANUFACTURING OR FORMULATION USE ONLY

Active Ingredient:

ECANA Mimic*............................................................................................................................................ 67.0%

Other Ingredients........................................................................................................................................... 33.0%

Total: 100.0%

* ECANA mimic is a blend of nature-identical components that substantially replicate the composition and activity of 

plant-produced Extract of Chenopodium ambrosioides near ambrosioides (ECANA).

KEEP OUT OF REACH OF CHILDREN

CAUTION

FIRST AID

If In Eyes

 Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove contact 

lenses, if present, after the first 5 minutes, then continue rinsing eye.

 Call a poison control center or doctor for further treatment advice.

If Swallowed

 Call poison control center or doctor for treatment advice.

 Have person sip a glass of water if able to swallow.

 Do not induce vomiting unless told to do so by the poison control center or doctor.

 Do not give anything by mouth to an unconscious person.

If on Skin or 

Clothing:

 Take off contaminated clothing. 

 Rinse skin immediately with plenty of water for 15-20 minutes.

 Call a poison control center or doctor for treatment advice. 

HOT LINE NUMBER

CHEMTREC (800) 424-9300 (24 hours)

Have the product container or label with you when calling a poison control center or doctor or going for treatment.

See side/back panels for additional precautionary statements

EPA Reg No. 69592-EU EPA Establishment No. 69592-MEX-1

Net Contents: 2.5 gallon (320 fl. oz.) (9.5 L)

 [4 gallon (15.2 L)]

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150
ECANA and ECANA Mimic Page 34 of 60

Biopesticides Registration Action Document

PRECAUTIONARY STATEMENTS

HAZARD TO HUMANS AND DOMESTIC ANIMALS

CAUTION: Harmful if swallowed. Causes moderate eye irritation. Avoid contact with eyes or clothing. 

Wash thoroughly with soap and water after handling and before eating, drinking, chewing gum or using 

tobacco. Prolonged or frequently repeated skin contact may cause allergic reactions in some individuals. 

Mixers, loaders and handlers must wear the appropriate personal protective equipment (PPE): coveralls, 

chemical-resistant gloves and protective eyewear. Remove and wash contaminated clothing before reuse.

ENVIRONMENTAL HAZARDS 

Do not discharge effluent containing this product into lakes, streams, ponds, estuaries, oceans or other waters 

unless in accordance with the requirements of a National Pollutant Discharge Elimination System (NPDES) 

permit and the permitting authority has been notified in writing prior to discharge. Do not discharge effluent 

containing this product to sewer systems without previously notifying the local sewage treatment plant 

authority. For guidance contact your State Water Board or Regional Office of the EPA. Do not contaminate 

water when disposing of equipment washwaters or rinsate. 

This pesticide is toxic to aquatic invertebrates.

PHYSICAL OR CHEMICAL HAZARDS

Combustible. Do not use or store near heat or open flame.

DIRECTIONS FOR USE

It is a violation of Federal law to use this product in a manner inconsistent with its labeling.

QRD 420 is intended for use in the formulation of insecticides and acaricides to be applied to field and 

container-grown plants in commercial nurseries, greenhouses, and lath- and shade houses, and labeled 

agricultural crops. 

This product may be used to formulate products for any additional uses not listed on the MP label if the 

formulator, user group, or grower has complied with U.S. EPA data submission requirements regarding the 

support of such uses. Products formulated from this technical material will require registration with the 

U.S. Environmental Protection Agency (EPA).

STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

Pesticide Storage: Store in a cool dry place. Avoid freezing.

Pesticide Disposal: To avoid wastes, use all material in this container by application according to label 

directions. If wastes cannot be avoided, offer remaining product to a waste disposal facility or pesticide 

disposal program (often such programs are run by state or local governments or by industry).

Do not contaminate water when disposing of equipment washwater or rinsate. Pesticide wastes may be toxic. 

Improper disposal of unused pesticide, washwater or rinse water is a violation of federal law. 

Container Disposal: Nonrefillable container. Do not reuse or refill this container

Triple rinse container (or equivalent) promptly after emptying. Triple rinse as follows: Empty the remaining 

contents into application equipment or a mix tank and drain for 10 seconds after the flow begins to drip. Fill 

the container 1⁄4 full with water and recap. Shake for 10 seconds. Pour rinsate into application equipment or a 

mix tank or store rinsate for later use or disposal. Drain for 10 seconds after the flow begins to drip. Repeat 

this procedure two more times. Then offer for recycling if available, or puncture and dispose of in a sanitary

landfill or by incineration. Do not burn unless allowed by state and local ordinances.
ECANA and ECANA Mimic Page 35 of 60

Biopesticides Registration Action Document

D. ECANA Mimic End Use Product Label

QRD 452

[Alternate Brand Names: REQUIEM®16.75EC, METRONOMETM II]

Master Label

Sub-Label A: Agricultural/Commercial Use

Sub-Label B: Greenhouse & Nursery/Commercial Use

Active Ingredient:

ECANA Mimic*.......................................................................................................................................... 16.75%

Other Ingredients......................................................................................................................................... 83.25%

Total: 100.0%

* ECANA mimic is a blend of nature-identical components that substantially replicate the composition and activity of 

plant-produced Extract of Chenopodium ambrosioides near ambrosioides (ECANA).

KEEP OUT OF REACH OF CHILDREN

CAUTION

EPA Reg No.: 69592- EL EPA Establishment No.: 69592-MEX-1

 

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150
ECANA and ECANA Mimic Page 36 of 60

Biopesticides Registration Action Document

QRD 452

(Alternate Brand Name: REQUIEM®16.75EC)

Sub-Label A

For Agricultural / Commercial Use Only

Not For Residential Use
ECANA and ECANA Mimic Page 37 of 60

Biopesticides Registration Action Document

REQUIEM®16.75EC

INSECTICIDE AND ACARICIDE FOR CONTROL OR SUPPRESSION OF APHIDS, THRIPS, WHITEFLIES,

PLANT-FEEDING MITES, MEALY BUGS, LEAFMINERS, LYGUS BUGS, SCALE, LEAF HOPPERS, AND 

LEPIDOPTEROUS INSECTS IN AGRICULTURAL CROPS.

NOT FOR RESIDENTIAL USE

Active Ingredient:

ECANA Mimic*..................................................................................................................................................16.75%

Other Ingredients.................................................................................................................................................83.25%

Total: 100.0%

* ECANA mimic is a blend of nature-identical components that substantially replicate the composition and activity of 

plant-produced Extract of Chenopodium ambrosioides near ambrosioides (ECANA).

KEEP OUT OF REACH OF CHILDREN

CAUTION

FIRST AID

If In Eyes

 Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove 

contact lenses, if present, after the first 5 minutes, then continue rinsing eye.

 Call a poison control center or doctor for further treatment advice.

In On Skin Or Clothing

 Take off contaminated clothing.

 Rinse skin immediately with plenty of water for 15-20 minutes.

 Call a poison control center or doctor for further treatment advice.

HOT LINE NUMBER 

CHEMTREC (800) 424-9300 (24 hours)

Have the product container or label with you when calling a poison control center or doctor or going for treatment.

See side/back panels for additional precautionary statements

EPA Reg No.: 69592- EL EPA Establishment No.: 69592-MEX-1

Net Contents: 2.5 gallon (320 fl. oz.) (9.46 L)

 

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150
ECANA and ECANA Mimic Page 38 of 60

Biopesticides Registration Action Document

PRECAUTIONARY STATEMENTS

HAZARD TO HUMANS AND DOMESTIC ANIMALS

CAUTION: Causes moderate eye irritation. Avoid contact with skin, eyes or clothing. Wash 

thoroughly with soap and water after handling and before eating, drinking, chewing gum or using 

tobacco. Remove and wash contaminated clothing before reuse. Prolonged or frequently repeated 

skin contact may cause allergic reactions in some individuals. Wear the appropriate Personal 

Protective Equipment (PPE).

PERSONAL PROTECTIVE EQUIPMENT

Applicators mixers, loaders and other handlers must wear:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material such as polyethylene or polyvinyl 

chloride

 Shoes plus socks

 Protective eyewear

 Coveralls for high-pressure handwand and groundboom applicators.

 For overhead exposure wear chemical resistant headgear.

 For greenhouse uses, applicators and other handlers must wear a NIOSH-approved respirator with 

an organic vapor (OV) cartridge or canister with any R, P or HE filter.

Follow the manufacturer’s instructions for cleaning/maintaining PPE. If no such instructions for 

washables, use detergent and hot water. Keep and wash PPE separately from other laundry.

Engineering Controls: When handlers use enclosed cabs in a manner that meets requirements listed in the 

Worker Protection Standard (WPS) for agricultural pesticides [40 CFR 170.240 (d) (4-6)], the handler 

PPE requirements may be reduced or modified as specified in the WPS.

USER SAFETY RECOMMENDATIONS

Users should: 

● Remove clothing immediately if pesticide gets inside. Then wash thoroughly and put on clean clothing. 

● Remove PPE immediately after handling this product. Wash the outside of gloves before removing. As soon as 

possible, wash thoroughly and change into clean clothing.

ENVIRONMENTAL HAZARDS 

This product is toxic to aquatic invertebrates. For terrestrial uses: Do not apply directly to water or to 

areas where surface water is present, or to intertidal areas below the mean high water mark. Do not 

contaminate water when disposing of equipment washwater or rinsate. 

Drift and runoff may be hazardous to aquatic organisms in water adjacent to treated areas. Do not 

apply when weather conditions favor drift or runoff from treated areas. Do not apply when a rain 

event is expected within 2 hours. Groundboom and air-blast applications must maintain a spray buffer 

of 15 feet from any water body. See the Directions for Use for details.

DIRECTIONS FOR USE

It is a violation of Federal law to use this product in a manner inconsistent with its labeling.

READ THE ENTIRE LABEL. USE STRICTLY IN ACCORDANCE WITH THE LABEL. 
ECANA and ECANA Mimic Page 39 of 60

Biopesticides Registration Action Document

FAILURE TO FOLLOW DIRECTIONS AND PRECAUTIONS ON THIS LABEL MAY RESULT 

IN CROP INJURY, POOR INSECT CONTROL, AND/OR ILLEGAL RESIDUES.

Do not apply this product in a way that will contact workers or other persons, either directly or 

through drift. Only protected handlers may be in the area during application. For any requirements 

specific to your state or tribe, consult the State or Tribal agency responsible for pesticide regulation.

AGRICULTURAL USE REQUIREMENTS

Use this product only in accordance with its labeling and with the Worker Protection Standard, 40

CFR Part 170. This Standard contains requirements for the protection of agricultural workers on 

farms, forests, nurseries, greenhouses and handlers of agricultural insecticides. It contains 

requirements for training, decontamination, notification and emergency assistance. It also contains 

specific instructions and exceptions pertaining to the statements on this label about personal 

protective equipment (PPE) and restricted-entry interval. The requirements in this box only apply to 

uses of this product that are covered by the Worker Protection Standard.

Do not enter or allow worker entry into treated areas during the restricted entry interval (REI) 

of 4 hours.

PPE required for early entry to treated areas that is permitted under the Worker Protection Standard 

and that involves contact with anything that has been treated, such as plants, soil or water is:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material such as polyethylene or polyvinyl 

chloride

 Shoes plus socks 

SHAKE WELL BEFORE USE 

GENERAL INFORMATION

REQUIEM®is a contact insecticide/acaricide for use in the control or suppression of many foliage 

feeding plants including aphids, thrips, plant-feeding mites, whiteflies, mealy bugs, leafminers, lygus 

bugs, scale, leaf hoppers, and lepidopterous insects infesting labeled crops. The emulsifiable

concentrate of REQUIEM must be mixed with water and applied as a foliar spray with ground 

equipment equipped for conventional insecticide spraying. 

MIXING AND APPLICATION INSTRUCTIONS

Do not apply this product by aerial application or through any type of irrigation system. 

REQUIEM is toxic to aquatic invertebrates. Users must maintain the following buffer zones: 

Groundboom and air-blast spray applicators must not apply within 15 feet of aquatic habitats 

(such as, but not limited to, lakes, reservoirs, rivers, streams, marshes, ponds, estuaries, and 

commercial fish ponds). Groundboom and air-blast applicators must also account for wind 

direction and speed. Only apply this product if the wind direction favors on-target deposition. 

Do not apply when the wind velocity exceeds 10 mph. Do not apply when a rain event is 

expected within 2 hours.
ECANA and ECANA Mimic Page 40 of 60

Biopesticides Registration Action Document

Shake container well before use. REQUIEM must be diluted with water for spray applications. 

Partially fill the spray tank with clean water, add the specified amount of REQUIEM to the tank, 

and finish filling the tank to the proper volume to obtain the desired application rate per acre as 

specified in the crop and pest tables listed below. Use at dilutions no more concentrated than 2% 

v/v. 

Compatibility: Do not combine REQUIEM in the spray tank with other pesticides, surfactants, 

adjuvants, or fertilizers if there has been no previous experience or use of the combination to 

show it is physically compatible, and non-injurious under your use conditions. 

Do not use REQUIEM in tank mixes or in a program or in rotation with products containing captan 

(with REQUIEM either preceding or following captan applications) in any crop. Use of REQUIEM 

with captan-containing products may result in phytotoxicity. 

Do not use REQUIEM in tank mixture combinations or in rotation with any product containing a 

label warning against using surfactants, methylated seed oils, oils or other adjuvants. 

As with many Emulsifiable Concentrate (EC) formulations, certain combinations can exacerbate 

phytotoxicity problems. Products which are phytotoxic when used alone may be more phytotoxic 

when used with an EC formulation such as REQUIEM. AgraQuest has not tested all varieties and 

cultivars of all crops and potential tank-mix or rotational combinations. 

To ensure crop safety, test REQUIEM in combination and in rotation with other products on a 

small portion of the crop. Wait at least 24-72 hours to evaluate results before applying to the entire 

field. If phytotoxicity is noted, and if it is determined that the level of phytotoxicity will result in 

economic losses to the crop, do not make the application to the crop.

To ensure physical compatibility of tank-mix combinations they must be evaluated prior to use. 

When products are physically compatible, a homogeneous solution is formed. To determine the 

physical compatibility of this product with other products, use a jar test. Using a quart jar, add the

proportionate amounts of the products to approximately one quart of water with agitation. Add 

dry formulations first, then flowables, then emulsifiable concentrates last. After thorough mixing, 

allow this mixture to stand for 15 minutes. If the mixture in the test jar remains uniform for 15 

minutes, the combination is compatible and can be used. If separation occurs (e.g. oils float to 

top, clumps of solids form, etc.), the combination is incompatible and do not use the 

mixture. Once compatibility has been proven, use the same procedure for adding required 

ingredients to the spray tank.

Use of Adjuvants: The use of adjuvants with REQUIEM is not required. 

Biological Control Agents: This product is compatible with the following biological control 

agents: Encarsia formosa, Amblyseius fallacis, Phytoseiulus persimilis, Orius insidiosus, Aphidius 

colemani, Zetzellia mali, Neoseiulus fallacies, Typhlodromus pyri, Stethorus punctum, lacewings, 

syrphid fly larvae, phytoseiid mite predators, aphid midges, lady beetles, and other predacious 

mites. Wait at least 2 hours after application before introducing new biological agents, or spray the 

evening before the day of introduction of new biological control agents.

AgraQuest is committed to the practice of sound resistance management programs that include 

rotation and tank mixing with other treatments having a variety of modes of actions.
ECANA and ECANA Mimic Page 41 of 60

Biopesticides Registration Action Document

APPLICATION

Use REQUIEM early in the pest cycle when local thresholds are reached or before insects reach 

damaging levels. REQUIEM is a contact insecticide and thorough coverage is necessary for 

optimum control. Use appropriate nozzles and nozzle configurations, along with sufficient spray

volume to achieve thorough coverage. Rates of REQUIEM may need to increase with increased 

insect populations and/or large plant canopies. It is not necessary to use a surfactant with 

REQUIEM.

REQUIEM can be applied with commonly used ground equipment. Use of air-blast spray

equipment may result in overhead exposure and requires the use of chemical-resistant headgear. 

When using REQUIEM with a new crop, test on a small area before applying to the entire field. Do 

not apply REQUIEM more than 10 times per crop production cycle. 

PREHARVEST INTERVAL

REQUIEM can be applied up to and including the day of harvest. However, note that the restricted 

entry interval (REI) is 4 hours.

SPRAY DRIFT PRECAUTIONS

Avoiding spray drift at the application site is the responsibility of the applicator. The interaction of 

many equipment- and weather–related factors determine the potential for spray drift. The applicator 

and the grower/treatment coordinator are responsible for considering all of these factors when

making decisions. Where states have more stringent regulations, they should be observed. Note: 

This section is advisory in nature and does not supersede the mandatory label requirements.

All ground application equipment must be properly maintained and calibrated using appropriate 

carriers.

Make ground applications when the wind velocity favors on target product deposition 

(approximately 3 to 10 mph). Do not apply when wind velocity exceeds 10 mph. Avoid applications 

when wind gusts approach 10 mph.

Risk of exposure to sensitive areas can be reduced by avoiding applications when wind direction is 

toward the area.

Low humidity and high temperatures increase the evaporation rate of spray droplets and therefore 

the likelihood of increased spray drift. Avoid spraying during conditions of low humidity and/or 

high temperature.

Do not make ground applications during temperature inversions. Inversions are characterized by

stable air and increasing temperatures with height above the ground. Mist or fog may indicate the 

presence of an inversion in humid areas. The applicator may detect the presence of an inversion by

producing smoke and observing a smoke layer near the ground surface.

FOR USE ON THE FOLLOWING CROPS

(REQUIEM has a 0-Day PreHarvest Interval (PHI) for all crops contained on this label. 

However, note that the restricted entry interval (REI) is 4 hours.)
ECANA and ECANA Mimic Page 42 of 60

Biopesticides Registration Action Document

Asparagus 

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Thrips

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Brassica (Cole) Leafy Vegetables

Including Broccoli, Broccoli Raab, Brussels Sprouts, Cabbage, Chinese Cabbage (Bok Choy

and Napa), Cauliflower, Cavalo, Collards, Kale, Kohlrabi, Mustard Greens, Rape Greens, 

and other Brassica leafy vegetable crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Cabbage aphid

 Green peach aphid

 Turnip aphid

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. 

Thrips will seek shelter in hard-to-reach parts of 

the plant. Use insecticide nozzles with sufficient 

water volume and spray pressure for thorough 

coverage. 

Whiteflies:

 Silverleaf whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Leafminers For Suppression: Begin treatments before 

thresholds have been reached. 

Bulb Vegetables

Including Garlic, Leek, Onion (dry bulb, green, and Welch), and Shallot and other bulb 

vegetable crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Leafminer

Maggots

1.5 - 4 Apply before pests reach damaging levels. Repeat 

applications as needed to maintain control. The 
ECANA and ECANA Mimic Page 43 of 60

Biopesticides Registration Action Document

Mites amount of REQUIEM per acre will depend on 

plant size, leaf canopy and pest pressure. Choose 

lower rates for light infestations and/or small 

plants and higher rates for heavy infestations 

and/or larger plants. 

Thrips:

 Onion thrips

 Western flower thrips

 Tobacco thrips

Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. 

Thrips will seek shelter hard-to-reach parts of 

the plant. Use insecticide nozzles with sufficient 

water volume and spray pressure for thorough 

coverage. 

Bush Berries

Including Blueberry, Currant, Gooseberry, Huckleberry, Elderberry, and other bush berry 

crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Leafhoppers

Leafrollers

Loopers

Lygus Maggots

Mites

Plum curculio

Thrips

 Western flower thrips

2 - 4 Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control The amount of REQUIEM per acre will 

depend on plant size, leaf canopy and pest 

pressure. Choose lower rates for light 

infestations and/or small plants and higher rates 

for heavy infestations and/or larger plants. 

Adjust equipment, water volume, and spray

pressure to ensure thorough coverage of infested 

parts of the crop.

Cranberry

Do not apply to flooded fields.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Leafhoppers

Leafrollers

Loopers

Thrips 

Wireworms

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Cane Berries

Including Blackberry (including varieties, cultivars, and/or hybrids), Loganberry, 

Raspberry, and other cane berry crops.

Pests and Application Rates:

Pests REQUIEM Remarks
ECANA and ECANA Mimic Page 44 of 60

Biopesticides Registration Action Document

(qts/acre)

Aphids

Leafhoppers

Leafrollers

Loopers

Sawfly

Whiteflies

Thrips

 Western flower thrips

2 - 4 Apply before pests reach damaging levels. Repeat 

applications as needed to maintain control. The 

amount of REQUIEM per acre will depend on 

plant size, leaf canopy and pest pressure. Choose 

lower rates for light infestations and/or small 

plants and higher rates for heavy infestations 

and/or larger plants. Adjust equipment, water 

volume, and spray pressure to ensure thorough 

coverage of infested parts of the crop.

Citrus Fruits

Including Grapefruit, Lemon, Lime, Orange, Tangerine, Citrus hybrids, Pummelo, and 

other citrus fruit crops. 

Pests and Application Rates: 

Pests REQUIEM

(qts/acre)

Remarks

Asian citrus psyllid

Lepidoptera larvae:

 Avocado leafroller

 Citrus peelminer

 Citrus Leafminer

 Cutworms

 Fruit tree leafroller

 Orange tortrix

Mites:

 Broad mite

 Citrus flat mite

 Citrus bud mite

 Citrus red mite

 Citrus rust mite

 Six-spotted spider mite

 Texas citrus mite

 Yuma spider mite

 Two-spotted spider mite

 Rust mite

Thrips:

 Citrus thrips

 Frankliniella spp. 

 Bean thrips

2 - 4 Treat when pests appear or in accordance with 

local economic thresholds. Repeat applications as 

needed to maintain control. The rate of 

REQUIEM will depend on tree size and pest 

pressure. Use a lower rate for light infestation 

and/or small trees and a higher rate for heavy

infestations and/or large trees. When applying to 

large trees, use higher water volumes to ensure 

thorough coverage. Do not exceed 2% v/v 

solution. 

Scale

 

Begin applications when thresholds are reached. 

Treat for scale when crawlers are present in the 

spring and fall. 
ECANA and ECANA Mimic Page 45 of 60

Biopesticides Registration Action Document

Cucurbit Vegetables

Including Cucumber, Citron melon, Edible Gourds, Gherkin, Muskmelons (including 

hybrids and/or cultivars of Cucumis melo), Pumpkin, Summer and Winter Squash, 

Watermelon, Cantaloupe, and other cucurbit vegetable crops.

Use of REQUIEM in tank mixes with some products including, but not limited to, those 

containing strobilurins or chlorothalonil may result in phytotoxicity. Do not apply products 

containing chlorothalonil within 4 days after application of REQUIEM. Do not apply 

REQUIEM for 30 days following an application of chlorothalonil.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

 Green peach aphid

 Melon aphid

Leafminers

Mites

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips

 Melon thrips

 Western flower thrips

Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use appropriate nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies:

 Greenhouse whitefly

 Sweetpotato whitefly

 Silverleaf whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Figs 

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Mites

Navel orangeworm

Scale

Vinegar flies

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Globe Artichoke 

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids 2 - 4 Begin applications when thresholds have been 
ECANA and ECANA Mimic Page 46 of 60

Biopesticides Registration Action Document

Leafhoppers

Plume moth

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Fruiting Vegetables

Including Eggplant, Ground Cherry, Pepino, Pepper, Tomatillo, Tomato, and other fruiting 

vegetable crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Green peach aphid

 Potato aphid

Leafhoppers

Mites

Tomato pinworm

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips:

 Western flower thrips

 Onion thrips

 Chili thrips

 Melon thrips

 Florida flower thrips

 Eastern flower thrips

Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use appropriate nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies:

 Silverleaf whitefly

 Greenhouse whitefly

 Bandedwinged whitefly

 Sweetpotato whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations 

have become established. Use the high rate 

when conditions are favorable for heavy

pressure. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 

Grape

Do not use REQUIEM on grapes east of the Rockies. Use of REQUIEM on grapes after fruit 

set can diminish waxy bloom.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Leafhoppers:

 Grape leafhopper

 Variegated leafhopper

2 - 4 For Suppression: Begin treatments before 

thresholds have been reached. 

Grape berry moth

Western grape skeletonizer

Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 
ECANA and ECANA Mimic Page 47 of 60

Biopesticides Registration Action Document

Mealy bug

Mites:

 Pacific spider mites

 Willamette spider mite

 Two-spotted spider mite

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. Adjust equipment, spray volume, and 

spray pressure to ensure thorough coverage of 

infested parts of the crop. 

Herbs and Spices

Including Allspice, Anise, Basil, Borage, Cardamom, Chive, Dill, Fennel (common), Juniper 

berry, Marjoram, Nutmeg, Pepper, Saffron, Sage, Tarragon, Vanilla, and other herb and 

spice crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Fleahoppers

Leafhoppers

Grasshoppers

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 

Hops

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Mites

Aphids

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Legume Vegetables (Succulent or Dried)

Including Beans, Green beans, Snap beans, Shell beans, Garbanzo beans, Lima beans, Peas, 

Chick peas, Split peas, Lentils and other legume vegetable crops.

Pests and Application Rates:

Pests REQUIEM

(qts./acre)

Remarks

Aphids

Leafhoppers

Lygus bugs

Mites

2 – 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 
ECANA and ECANA Mimic Page 48 of 60

Biopesticides Registration Action Document

plant canopy.

Thrips:

 Western Flower 

Thrips

 Melon Thrips 

 

Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. 

Thrips will seek shelter in hard-to-reach parts of 

the plant. Use insecticide nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations 

have become established. Use the high rate when 

conditions are favorable for heavy pressure.

Leafminers (Liriomyza spp.) For suppression: Begin treatments when 

leafminers are seen in the crop. Continue 

applications as needed.

Mint

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Mites

Aphids

Thrips

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Leafy Vegetables

Including Lettuce, Arugula, Celery, Spinach, Parsley, Radicchio, Florence Fennel, Romaine, 

Swiss Chard, and other leafy vegetable crops. 

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Foxglove aphid

 Lettuce aphid

 Lettuce root aphid

 Cabbage aphid

 Turnip aphid

 Potato aphid

 Green peach aphid

Leafhoppers

Lygus bugs

Mites (except bulb mite)

Psyllid

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips Begin applications as soon as thrips are seen in 
ECANA and ECANA Mimic Page 49 of 60

Biopesticides Registration Action Document

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use appropriate nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies:

 Silverleaf whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 

Olives

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Mites

Scale

Thrips

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants.

Okra

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Leafhoppers

Mites

Tomato pinworm

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips:

 Melon thrips

 Western Flower thrips

 Chili thrips

 Florida Flower thrips

Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use insecticide nozzles with sufficient 

water volume and spray pressure for thorough 

coverage. 

Whiteflies Apply before pests reach damaging levels. Repeat 

applications as needed to maintain control. Do not 

wait until heavy populations have become 

established. Use the high rate when conditions are 

favorable for heavy pressure. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 
ECANA and ECANA Mimic Page 50 of 60

Biopesticides Registration Action Document

Leaves of Root and Tuber Vegetables

Including Carrot, Sweet Potato, Cassava, Beets, Celeriac, Radish, Turnip, Parsnip, and other 

leaves of root and tuber crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids

Leafhoppers

Mites

Psylla

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use appropriate nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies Apply before pests reach damaging levels.

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 

Pome Fruits

Including Apple, Crabapple, Loquat, Mayhaw, Pear, Quince, and other pome fruit crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Green apple aphid

 Wooly apple aphid

 Cotton aphid

 Bean aphid

 Rosy apple aphid

 Spirea aphid

 Green peach aphid

Mites:

 Brown mite

 Pear rust mite

 Pear leaf blister mite

 European red mite

 Two-spotted spider mite

 Pacific spider mite

 McDaniel spider mite 

Leafhoppers

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large plant 

canopy.
ECANA and ECANA Mimic Page 51 of 60

Biopesticides Registration Action Document

Mealy bugs

Pear psyllid

Plum curculio

Scale

Thrips

Codling moth 

Oriental fruit moth

Tufted apple moth 

Leafminers (lepidopteran):

 Spotted tentiform

 Western tentiform

Leafrollers:

 Oblique-banded

 Pandernis

Monitor the moth flights and the infestation 

densities of the larval stages. Repeat applications 

as necessary to maintain control. 

Root / Tuber and Corm Vegetables

Including Carrot, Potato, Sweet Potato, Cassava, Beets, Ginger, Horseradish, Radish, 

Ginseng, Turnip, True Yam, and other root/tuber and corm crops.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Potato aphid

 Green peach aphid

Leafhoppers

Mites

Psylla:

 Potato psyllid

2 - 4 Begin treatments when thresholds have been 

reached. 

Thrips Begin applications as soon as thrips are seen in 

the crop. Continue applications as needed. Thrips 

will seek shelter in hard-to-reach parts of the 

plant. Use appropriate nozzles with sufficient 

water volume and spray pressure for thorough 

coverage.

Whiteflies:

 Silverleaf whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Leafminers (Liriomyza spp.) For Suppression: Begin treatments before 

thresholds have been reached. 

Stone Fruits

Including Apricot, Cherry, Nectarine, Peach, Plum, Plumcot, Prune, and other stone fruit crops

Pests and Application Rates:

Pests REQUIEM Remarks
ECANA and ECANA Mimic Page 52 of 60

Biopesticides Registration Action Document

(qts/acre)

Aphids

Cherry fruit fly

Leafhoppers

Leafminers

Mites

Plum curculio

Scale

Western cherry fruit fly

Thrips

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large plant 

canopy. 

Leafrollers 

Leafminers

Peach twig borer

Monitor the moth flights and the infestation 

densities of the larval stages. Repeat applications 

as necessary to maintain control. 

Strawberry

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Green peach aphid

 Melon aphid

 Potato aphid

 Strawberry aphid

Leafhoppers

Lygus bug 

Mites:

 Two-spotted spider mite

 Carmine spider mite

 Cyclamen mite

 Pacific spider mite

Thrips:

 Western flower thrips

1 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Whiteflies:

 Silverleaf whitefly

 Greenhouse whitefly

 Strawberry whitefly

Apply before pests reach damaging levels. 

Repeat applications as needed to maintain 

control. Do not wait until heavy populations have 

become established. Use the high rate when 

conditions are favorable for heavy pressure. 

Tropical Fruits

Including Avocado, Banana, Kiwi, Mango, Papaya, Plantains, Pineapple, and other tropical 

fruits.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Avocado leafroller

Citrus peelminer 

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 
ECANA and ECANA Mimic Page 53 of 60

Biopesticides Registration Action Document

Cutworms

Fruit tree leafroller

Naval orange worm 

Orange tortrix

Western tussock moth

Mites

Thrips

Scale

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large 

plants. 

Tree Nuts

Including Almond, Beech nut, Cashew, Chestnut, Filbert, Hickory nut, Pecan, Walnut, and 

other tree nut crops.

Do not use of REQUIEM in tank mixes with chlorothalonil. Do not apply products containing 

chlorothalonil within 30 days after application of REQUIEM. Do not apply REQUIEM for 30 

days following an application of chlorothalonil.

Pests and Application Rates:

Pests REQUIEM

(qts/acre)

Remarks

Aphids:

 Walnut aphid

 Dusky-veined aphid

 Rosy apple aphid

 Green peach aphid

 Spirea aphid

 Yellow aphid

 Black pecan aphid

 Black-margined aphid

 Yellow pecan aphid

Fall webworm

Scale

Walnut husk fly

Mites:

 Brown mite

 Peach silver mite

 Citrus flat mite

 Pacific spider mite

 European red mite

 Two-spotted spider mite

 Strawberry spider mite

2 - 4 Begin applications when thresholds have been 

reached. The rate of REQUIEM will depend on 

plant size and pest pressure. Use a lower rate for 

light infestation and/or small plant canopy and a 

higher rate for heavy infestations and/or large plant 

canopy. 

Leafminers

Leafrollers

Peach twig borer

Pecan nut case bearer

Monitor the moth flights and the infestation 

densities of the larval stages. Repeat applications 

as necessary to maintain control. 
ECANA and ECANA Mimic Page 54 of 60

Biopesticides Registration Action Document

STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

 Pesticide Storage: Store in a cool dry place. Avoid freezing. 

 Pesticide Disposal: To avoid wastes, use all material in this container by application according to 

label directions. If wastes cannot be avoided, offer remaining product to a waste disposal facility or 

pesticide disposal program (often such programs are run by state or local governments or by

industry).

 Do not contaminate water when disposing of equipment washwater or rinsate. Pesticide wastes 

may be toxic. Improper disposal of unused pesticide, washwater or rinse water is a violation of 

federal law. 

 Container Disposal: Non-refillable container. Do not reuse or refill this container

 Triple rinse container (or equivalent) promptly after emptying. Triple rinse as follows: Empty the 

remaining contents into application equipment or a mix tank and drain for 10 seconds after the flow 

begins to drip. Fill the container 1⁄4 full with water and recap. Shake for 10 seconds. Pour rinsate 

into application equipment or a mix tank or store rinsate for later use or disposal. Drain for 10 

seconds after the flow begins to drip. Repeat this procedure two more times. Then offer for 

recycling if available, or puncture and dispose of in a sanitary landfill or by incineration. Do not 

burn unless allowed by state and local ordinances.

WARRANTY AND DISCLAIMER STATEMENT

AgraQuest warrants to those persons lawfully purchasing this product that at the time of the first 

sale of this product by Seller that this product conformed to its description and was reasonably fit 

for the purposes stated on the label when used in accordance with Seller’s directions. Buyers and 

users of this product assume the risk of any use contrary to such directions. EXCEPT AS 

PROVIDED ELSEWHERE IN WRITING CONTAINING AN EXPRESS REFERENCE TO 

THIS WARRANTY AND LIMITATION OF DAMAGES, SELLER MAKES NO OTHER 

EXPRESS OR IMPLIED WARRANTY OR GUARANTY, INCLUDING ANY OTHER 

EXPRESS OR IMPLIED WARRANTY OF FITNESS OR OF MERCHANTABILITY AND NO 

AGENT OF SELLER IS AUTHORIZED TO DO SO. Except to the extent prohibited by

applicable law, AgraQuest offers this product with the following conditions: 1) buyers and users 

of this product assume the risk of any storage, handling or use contrary to AgraQuest’s label and 

directions and 2) AgraQuest’s liability shall in no case exceed the purchase price of the applicable 

AgraQuest product.

REQUIEM is a registered trademark of AgraQuest.

QRD 452
ECANA and ECANA Mimic Page 55 of 60

Biopesticides Registration Action Document

(Alternate Brand Name: METRONOMETM II)

Sub-Label B: For Greenhouse & Nursery / Commercial Use Only

METRONOMETM II

Insecticide and Acaricide

FOR USE ON NON-FOOD ORNAMENTALS (TREES, SHRUBS, AND BEDDING PLANTS)

NOT FOR RESIDENTIAL USE

Active Ingredient:

ECANA Mimic*..................................................................................................................................................16.75%

Other Ingredients.................................................................................................................................................83.25%

Total: 100.0%

* ECANA mimic is a blend of nature-identical components that substantially replicate the composition and activity of 

plant-produced Extract of Chenopodium ambrosioides near ambrosioides (ECANA).

KEEP OUT OF REACH OF CHILDREN

CAUTION

FIRST AID

If In Eyes

 Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove 

contact lenses, if present, after the first 5 minutes, then continue rinsing eye.

 Call a poison control center or doctor for further treatment advice.

In On Skin Or Clothing

 Take off contaminated clothing.

 Rinse skin immediately with plenty of water for 15-20 minutes.

 Call a poison control center or doctor for further treatment advice.

HOT LINE NUMBER 

CHEMTREC (800) 424-9300 (24 hours)

Have the product container or label with you when calling a poison control center or doctor or going for treatment.

See side/back panels for additional precautionary statements

EPA Reg No. 69592-EL EPA Establishment No. 69592-MEX-1

Net Contents: 1 gallon (128 fl. oz.) (3.8 L)

 [2.5 gallon (320 fl. oz.) (9.46 L)]

Manufactured By:

AgraQuest, Inc.

1540 Drew Avenue

Davis, California 95618

(530) 750-0150
ECANA and ECANA Mimic Page 56 of 60

Biopesticides Registration Action Document

PRECAUTIONARY STATEMENTS

HAZARD TO HUMANS AND DOMESTIC ANIMALS

CAUTION: Causes moderate eye irritation. Avoid contact with skin, eyes or clothing. Wash 

thoroughly with soap and water after handling and before eating, drinking, chewing gum or using 

tobacco. Remove and wash contaminated clothing before reuse. Prolonged or frequently repeated 

skin contact may cause allergic reactions in some individuals. Wear the appropriate Personal 

Protective Equipment (PPE).

PERSONAL PROTECTIVE EQUIPMENT

Applicators mixers, loaders and other handlers must wear:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material

 Shoes plus socks

 Protective eyewear

 Coveralls for high-pressure handwand and groundboom applicators.

 For greenhouse uses, applicators and other handlers must wear a NIOSH-approved respirator with 

an organic vapor (OV) cartridge or canister with any R, P or HE filter.

Follow the manufacturer’s instructions for cleaning/maintaining PPE. If no such instructions for 

washables, use detergent and hot water. Keep and wash PPE separately from other laundry.

USER SAFETY RECOMMENDATIONS

Users should: 

● Remove clothing immediately if pesticide gets inside. Then wash thoroughly and put on clean clothing. 

● Remove PPE immediately after handling this product. Wash the outside of gloves before removing. As soon as 

possible, wash thoroughly and change into clean clothing.

ENVIRONMENTAL HAZARDS 

This product is toxic to aquatic invertebrates. For terrestrial uses: Do not apply directly to water or to 

areas where surface water is present, or to intertidal areas below the mean high water mark. Do not 

contaminate water when disposing of equipment washwater or rinsate. 

Drift and runoff may be hazardous to aquatic organisms in water adjacent to treated areas. Do not 

apply when weather conditions favor drift or runoff from treated areas. Do not apply when a rain 

event is expected within 2 hours. Air blast applications must maintain a spray buffer of 15 feet from 

any waterbody. See the Directions for Use for details.

DIRECTIONS FOR USE

It is a violation of Federal law to use this product in a manner inconsistent with its labeling.

READ THE ENTIRE LABEL. USE STRICTLY IN ACCORDANCE WITH THE LABEL. 

FAILURE TO FOLLOW DIRECTIONS AND PRECAUTIONS ON THIS LABEL MAY RESULT 

IN CROP INJURY, POOR INSECT CONTROL, AND/OR ILLEGAL RESIDUES.

Do not apply this product in a way that will contact workers or other persons, either directly or through 

drift. Only protected handlers may be in the area during application. For any requirements specific to 
ECANA and ECANA Mimic Page 57 of 60

Biopesticides Registration Action Document

your state or tribe, consult the State or Tribal agency responsible for pesticide regulation.

AGRICULTURAL USE REQUIREMENTS

Use this product only in accordance with its labeling and with the Worker Protection Standard, 40 CFR Part 170. 

This Standard contains requirements for the protection of agricultural workers on farms, forests, nurseries, 

greenhouses and handlers of agricultural insecticides. It contains requirements for training, decontamination, 

notification and emergency assistance. It also contains specific instructions and exceptions pertaining to the 

statements on this label about personal protective equipment (PPE) and restricted-entry interval. The requirements 

in this box only apply to uses of this product that are covered by the Worker Protection Standard.

Do not enter or allow worker entry into treated areas during the restricted entry interval (REI) of 

4 hours.

PPE required for early entry to treated areas that is permitted under the Worker Protection Standard and that 

involves contact with anything that has been treated, such as plants, soil or water is:

 Long-sleeved shirt and long pants

 Chemical-resistant gloves made of any waterproof material

 Shoes plus socks 

NON-AGRICULTURAL USE REQUIREMENTS

The requirements in this box apply to uses that are NOT within the scope of the Worker Protection Standard 

for agricultural pesticides (40 CFR Part 170). The WPS applies when this product is used to produce 

agricultural plants on farms, nurseries or green houses. 

Do not enter treated areas without protective clothing until spray has dried. Refer to Personal 

Protective Equipment section under the Precautionary Statements section.

GENERAL INFORMATION

METRONOMETM is an Emulsifiable Concentrate (EC) Insecticide and Acaricide for Use on Field 

and Container-grown Non-Food Ornamental Plants in Commercial Nurseries, Greenhouses, and Lathand Shade-houses. Not For Residential Use.

METRONOME is a contact insecticide/acaricide for use in the control of aphids, plant-feeding mites,

whiteflies, mealy bugs, leafminers, thrips, fungus gnats, and tarnished plant bugs. METRONOME can

be applied to field and container-grown non-food ornamentals – trees, Christmas trees, shrubs, 

flowers, annual and perennial bedding plants, potted flowers, and tropical plants.

MIXING AND APPLICATION INSTRUCTIONS

Do not apply this product by aerial application or through any type of irrigation system. 

METRONOME is toxic to aquatic invertebrates. Users must maintain the following buffer zones: 

Airblast spray applicators must not apply within 15 feet of aquatic habitats (such as, but not limited 

to, lakes, reservoirs, rivers, streams, marshes, ponds, estuaries, and commercial fish ponds). 

Groundboom and airblast applicators must also account for wind direction and speed. Only apply this 

product if the wind direction favors on-target deposition. Do not apply when the wind velocity

exceeds 10 mph. Do not apply when a rain event is expected within 2 hours.

Shake container well before use. METRONOME must be diluted with water for spray applications. 

Partially fill the spray tank with clean water, add the specified amount of METRONOME to the tank 
ECANA and ECANA Mimic Page 58 of 60

Biopesticides Registration Action Document

(see table below), and finish filling the tank to the desired volume to obtain the proper spray

concentration for the desired application rate per acre.

Gallons Water 

per Acre

METRONOME gallons

0.5% solution

METRONOME gallons

1% solution

25 0.12 (16 fl oz) 0.25 (32 fl oz)

50 0.25 (32 fl oz) 0.5 (64 fl oz)

75 0.37 (48 fl oz) 0.75 (96 fl oz)

100 0.5 1

 For smaller quantities, mix at a rate of 0.65 – 1.3 fl. oz. METRONOME 

per 1 gallon of water.

Apply on a 7- to 14-day schedule as necessary. Apply METRONOME at first sign of insect pressure 

or plant-feeding mite infestation. Use higher application rate under conditions of heavy pest pressure. 

Uniform coverage of both upper and lower leaves is critical for effective pest control. Avoid 

overspraying to the point of excessive runoff. Do not apply METRONOME more than 10 times per 

year. 

METRONOME can be applied with commonly used ground equipment, hose-end, pressurized, 

greenhouse, and hand-held sprayers. 

Biological Control Agents: This product is compatible with the following biological control agents: 

Encarsia formosa, Amblyseius fallacis, Phytoseiulus persimilis, Orius insidiosus, Aphidius colemani, 

Zetzellia mali, Neoseiulus fallacies, Typhlodromus pyri, Stethorus punctum, lacewings, syrphid fly

larvae, phytoseiid mite predators, aphid midges, lady beetles, and other predacious mites. Wait at 

least 2 hours after application before introducing new biological agents, or spray the evening before 

the day of introduction of new biological control agents.

AgraQuest is committed to the practice of sound resistance management programs that include 

rotation and tank mixing with other treatments having a variety of modes of actions.

Compatibility: Do not combine METRONOME in the spray tank with other pesticides, surfactants, 

adjuvants, or fertilizers if there has been no previous experience or use of the combination to show it 

is physically compatible, and non-injurious under your use conditions.

Do not use METRONOME in tank mixes or in a program or in rotation with products containing 

captan (with METRONOME either preceding or following captan applications) in any crop. Use of 

METRONOME with captan-containing products may result in phytotoxicity. 

Do not use METRONOME in tank mixture combinations or in rotation with any product containing a 

label warning against using surfactants, methylated seed oils, oils or other adjuvants. 

As with many Emulsifiable Concentrate (EC) formulations, certain combinations can exacerbate 

phytotoxicity problems. Products which are phytotoxic when used alone may be more phytotoxic 

when used with an EC formulation such as METRONOME. AgraQuest has not tested all varieties and

cultivars of all crops and potential tank-mix or rotational combinations.

To ensure physical compatibility of tank-mix combinations they must be evaluated prior to use. When 

products are physically compatible, a homogeneous solution is formed. To determine the physical 
ECANA and ECANA Mimic Page 59 of 60

Biopesticides Registration Action Document

compatibility of this product with other products, use a jar test. Using a quart jar, add the 

proportionate amounts of the products to approximately one quart of water with agitation. Add dry

formulations first, then flowables, then emulsifiable concentrates last. After thorough mixing, allow 

this mixture to stand for 15 minutes. If the mixture in the test jar remains uniform for 15 minutes, the 

combination is compatible and can be used. If separation occurs (e.g. oils float to top, clumps of 

solids form, etc.), the combination is incompatible and do not use the mixture. Once compatibility has 

been proven, use the same procedure for adding required ingredients to the spray tank. Test the 

combination on a small number of plants to check for phytotoxicity before treating large areas. Do not 

use the combination if adverse effects are observed. 

PLANTS EVALUATED FOR PHYTOTOXICITY

METRONOME has been evaluated alone in a wide range of crops and ornamentals. However, since 

testing on all varieties of all ornamentals is not feasible, testing a small portion of the area to be 

treated for phytotoxicity is required before treating the entire area. Further, all possible combinations 

or sequences of pesticide sprays, including other fertilizers, surfactants, adjuvants and other 

pesticides, have not been tested, thus testing for compatibility and phytotoxicity of spray mixtures is 

required. Use the product in accordance with all label use directions. It is further required that spray

equipment used to apply METRONOME be thoroughly cleaned before use. 

Phytotoxicity has occasionally been observed following the use of this product on flowers of 

Cyclamen, Gerbera, Begonia, and Impatiens. Do not use METRONOME on flowering Cyclamen, 

Gerbera, Begonia, and Impatiens. 

STORAGE AND DISPOSAL

Do not contaminate water, food or feed by storage or disposal.

Pesticide Storage: Store in a cool dry place. Avoid freezing. 

Pesticide Disposal: To avoid wastes, use all material in this container by application according to label directions. 

If wastes cannot be avoided, offer remaining product to a waste disposal facility or pesticide disposal program (often 

such programs are run by state or local governments or by industry).

Do not contaminate water when disposing of equipment washwater or rinsate. Pesticide wastes may be toxic. 

Improper disposal of unused pesticide, washwater or rinse water is a violation of federal law. 

Container Disposal: Non-refillable container. Do not reuse or refill this container

Triple rinse container (or equivalent) promptly after emptying. Triple rinse as follows: Empty the remaining 

contents into application equipment or a mix tank and drain for 10 seconds after the flow begins to drip. Fill the 

container 1⁄4 full with water and recap. Shake for 10 seconds. Pour rinsate into application equipment or a mix tank 

or store rinsate for later use or disposal. Drain for 10 seconds after the flow begins to drip. Repeat this procedure 

two more times. Then offer for recycling if available, or puncture and dispose of in a sanitary landfill or by

incineration. Do not burn unless allowed by state and local ordinances.

WARRANTY AND DISCLAIMER STATEMENT

AgraQuest warrants to those persons lawfully purchasing this product that at the time of the first sale of this 

product by Seller that this product conformed to its description and was reasonably fit for the purposes stated on 

the label when used in accordance with Seller’s directions. Buyers and users of this product assume the risk of 

any use contrary to such directions. EXCEPT AS PROVIDED ELSEWHERE IN WRITING CONTAINING 

AN EXPRESS REFERENCE TO THIS WARRANTY AND LIMITATION OF DAMAGES, SELLER 

MAKES NO OTHER EXPRESS OR IMPLIED WARRANTY OR GUARANTY, INCLUDING ANY 

OTHER EXPRESS OR IMPLIED WARRANTY OF FITNESS OR OF MERCHANTABILITY AND NO 

AGENT OF SELLER IS AUTHORIZED TO DO SO. Except to the extent prohibited by applicable law, 

AgraQuest offers this product with the following conditions: 1) buyers and users of this product assume the risk 

of any storage, handling or use contrary to AgraQuest’s label and directions and 2) AgraQuest’s liability shall in 
ECANA and ECANA Mimic Page 60 of 60

Biopesticides Registration Action Document

no case exceed the purchase price of the applicable AgraQuest product.

METRONOME is a registered trademark of AgraQuest.