Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A1 -1 

## Appendix A 1. Florida Waterbody Identification Numbers (WBIDs) by EPA Nutrient Watershed Region (NWR)

Appendix A1 contains a list of Florida waterbody identification numbers (WBIDs) organized by EPA’s Nutrient Watershed Region (NWR). This list is based on FDEP’s WBID map located at FDEP’s GIS site (http://www.dep.state.fl.us/gis/datadir.htm, accessed March 2010). The list is being provided in electronic form on CD. For a copy of t he CD, please contact EPA’s docket office. The docket identification number is EPA- HQ

- OW -2009-0596.
The Office of Water (OW) Docket Center is open from 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding legal holidays. The OW Docket Center telephone number is 202-566-1744 and the Docket address is OW Docket, EPA West, Room 3334, 1301 Constitution Ave., NW, Washington, DC 20004.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A2 -1 

## Appendix A2

. FDEP’s Process for Assessing Data Usability

# Process for Assessing Data Usability

DEP-EA 001/07

Florida Department of Environmental Protection 2600 Blair Stone Road Tallahassee, FL 32399-2400

Bureau of Standards and Special Projects Environmental Assessment Section

March 31, 2008Process for Assessing Data Usability DEP-EA 001/07



### Table of Contents
1. Introduction ........................................................................................................................... 1 1.1. The Data Assessment Process ..................................................................................... 1 1.2. Evaluating Data Quality Indicator Failures .................................................................... 1 2. Data Quality Indicators (DQI) ................................................................................................ 2 3. Laboratory Control Sample or Spike (LCS) ........................................................................... 2 3.1. General Requirements .................................................................................................. 2 3.2. Evaluation of LCS Recovery ......................................................................................... 3 4. Matrix Spikes (MS) ................................................................................................................ 3 4.1. General Requirements .................................................................................................. 3 4.2. Evaluation of MS Recovery ........................................................................................... 4 5. Surrogate Spikes ................................................................................................................... 5 6. LCS Duplicates or Replicates (LCSD) and Matrix Spike Duplicates (MSD) ......................... 5 6.1. General Requirements .................................................................................................. 5 6.2. Evaluation of Duplicates ................................................................................................ 5 7. Sample Duplicates (SD) ........................................................................................................ 6 7.1. General Requirements .................................................................................................. 6 7.2. Evaluation of Sample Duplicates ................................................................................... 6 8. Calibrations ........................................................................................................................... 7 8.1. Initial Calibration ............................................................................................................ 7 8.2. Calibration Verification (CV) .......................................................................................... 7 9. Method Blanks or Other Analytical Blanks - .......................................................................... 8 10. Field Quality Control Blanks (Trip Blanks, Field Blanks or Equipment Blanks .................. 9 11. Holding Times ................................................................................................................... 9 12. Quality Control Check Samples ...................................................................................... 10 12.1. BOD Analyses ............................................................................................................. 10 12.2. Chlorophyll Analyses ................................................................................................... 10 12.3. Matrix-Specific Evaluation for Known or Suspected Interferences .............................. 11 13. Sample Preservation Checks .......................................................................................... 12 14. Evaluation of the Reported MDL ..................................................................................... 12 15. Evaluation of the Reported PQL ..................................................................................... 12 16. Evaluation of Reversals (Parts vs. Whole Comparison ................................................... 12 17. General Principles of Data Quality Assessment .............................................................. 13 18. Procedure for Data Usability Determinations .................................................................. 15 19. Summary of the Data Usability Assessment Process ..................................................... 16

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### Process for Assessing Data Usability DEP-EA 001/07

1. 

### Introduction - The intent of this document is to outline the process to be used by the Department when evaluating Data Quality Indicators (DQI) and determining the usability of analytical data. 1.1. The Data Assessment Process - Determining if data are usable for a particular purpose is a complex task, requiring a reasonabl e and balanced evaluation of many factors. The procedural components of the usability assessment must be performed by auditors with sufficient scientific expertise in environmental data verification and validation, and include, but are not limited to: Understanding the purpose for auditing the data (project or program data quality objectives provide the context for the audit);

• Identifying the set of data to be audited, the types of analytes or parameters in the data set and the reported values (e.g., concentration) for the analytical results for all components;

• Determining the relationship between each analytical result, the associated decision or action level (e.g., water quality standard or clean up target) and the laboratory’s quantitation limit;

• Evaluating the documented calibration, quality control and other supporting data against designated Data Quality Indicators;

• Establishing the pattern, frequency, and magnitude of any failures or other deficiencies associated with the results;

• Determining the extent to which the audited data set fulfills the Data Quality Objectives of the project or Program;

• Evaluating corroborative data (e.g., performance tests, data from other laboratories);

• Providing usability recommendations to the Program data users. 1.2. Evaluating Data Quality Indicator Failures - Exceeding the acceptance criteria for one or more Data Quality Indicators does not necessarily mean the data are unusable. The factors mentioned in 1.1 above must be systematically evaluated before a usability decision can be made 1.2.1. The purpose for which the analytical data were collected can vary widely, and may include such diverse activities as: initial screening or scoping studies, permit compliance monitoring, assessing waters for Total Maximum Daily Load development or determining whether a permitted waste facility has met “clean closure” site contamination assessment requirements. 1.2.2. A Data Quality Indicator failure that is acceptable for a screening study may not be acceptable for declaring a site free from contamination.

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### 1.2.3. In practical terms, it is not possible in this document to discuss each potential scenario that might be associated with a data usability recommendation for a particular purpose. Therefore, the factors affecting this decision (outlined in 1.1 and 1.2, above) and the thought process applied to the data usability assessment are addressed by the general principles and examples listed below.
2. 

### Data Quality Indicators (DQI) - The following Data Quality Indicators (Sections 3.0 – 16) are targets used in the context of assessing data usability. Depending on a given situation, not all DQIs are applicable to the specific assessment project.

Example: To be considered usable, a data submittal to DEP consisting only of high sample concentrations (with no values near the PQL) need not be associated with records demonstrating evaluation or verification of the PQL.
2.1. The application of DQIs to sample results presumes the random occurrence of nonroutine criteria failures to be an expected fact associated with all analyses. The frequency of DQI failures are considered as part of the overall data usability assessment in order to determine those instances where routine or syst ematic failures indicate a significant data usability problem. 2.2. Use of DQIs with Other Published Criteria - Except as discussed otherwise in this document, sample results are evaluated for usability based on the procedural requirements and performance criteria established by the reported analytical methods, applicable project data quality objectives, applicable regulations, applicable NELAC Quality Systems standards and applicable DEP SOPs for field and laboratory activities (DEP-SOP-001/01 and DEP-SOP-002/01). 2.3. Use of Records to Assess Data Usability - As applicable to the data usability assessment process, any record associated with a reported sample result or set of sample results may be audited, per 62-160.240 & .340, FAC. Both original (“raw”) and reduced or summarized versions of data records are inspected in order to determine acceptance of the procedures and performance criteria that are used to generate and evaluate the sample data and associated quality control activities. 2.4. The presence or absence of critical, archival records that support the sample results is considered when determining whether suffici ent documentation is available to assess the usability of the data.
3. 

### Laboratory Control Sample or Spike (LCS) –In order of preference, LCS data are evaluated rather than matrix spike data (MS), if both types of data are generated for the associated sample preparation batch. However, the MS data are also used to evaluate the recoveries of the analytes for the parent sample and may affect the usability of the parent sample result. 3.1. General Requirements 3.1.1. An LCS result is linked or associated with all applicable reported sample results for the same preparation batch or analytical sequence of 20 samples or less. 3.1.2. The LCS contains all of the analytes of interest for the method or project, or the mix of analytes in the LCS is rotated at a routine frequency as suggested in the NELAC Quality Systems standard in Appendix D. 3.1.3. If a preparation step (such as digestion or extraction) is required for the analysis method, the LCS is prepared identically with and at the same time as the associated samples in the preparation batch. 3.1.4. The LCS is analyzed or reanalyzed within a verified calibration bracket (analytical sequence see Section 8.2.2 for the definition).

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### 3.1.5. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses. 3.1.6. The concentration of the LCS is analyzed to be within the calibration range for the instrument as established by the concentrations of the standards used for the initial calibration and continuing calibration verifications. 3.1.7. Where applicable, the LCS is prepared at the method-specified concentration or at a concentration appropriate for the project data quality objective. 3.2. Evaluation of LCS Recovery - Where applicable, the control limits for the LCS are established by the laboratory for a specified matrix at ± 3X the standard deviation of the mean recovery of the cumulative LCS analyses at a specified concentration. Alternatively, the recovery control limits are established by linear regression for a range of concentrations using cumulative recovery data. The mean recovery is calculated or regression analysis is applied after outliers are eliminated from the recovery data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also censored from the data set. The mean recovery is recalculated or linear regression is reanalyzed if trend monitoring of individual LCS re coveries indicates that a systematic bias has developed and corrective action is needed. 3.2.1. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 3.2.2. Where applicable, criteria established by rule, reported method or project data quality objectives are used to evaluate the LCS recoveries. 3.2.3. If no other criteria are applicable, the following control limits are used to evaluate the LCS recoveries: 80% - 120% (water) for Nitrate, Nitrite, Nitrate + Nitrite, Ammonium, TKN, Total Phosphorus, Orthophosphate 85% - 115% (water) for Metals Laboratory Control Limits for: All Organic analytes for the LCS in any matrix All other analytes amenable to spiking for the LCS in water All analytes amenable to spiking for the LCS in non-aqueous matrices 3.2.4. When applicable, sample results reported with failed LCS recoveries are documented with a valid explanation of the systematic error that occurred. 3.2.5. Analytes or methods technically not amenable to spiking for recovery determinations are not evaluated.
4. 

### Matrix Spikes (MS) - Method-specified criteria are used to evaluate the MS, where applicable. If no LCS data is available for the sample preparation batch or analytical sequence being evaluated, the MS data is used to evaluate method control for all of the associated samples in the batch or sequence. 4.1. General Requirements 4.1.1. The MS contains all of the analytes of interest for the method or project, or the mix of analytes in the MS is rotated at a routine frequency as suggested in the NELAC Quality Systems standard in Appendix D. 4.1.2. If a preparation step (such as digestion or extraction) is required for the analysis method, the MS is prepared identically with and at the same time as the associated samples in the preparation batch.

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### 4.1.3. The MS is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 4.1.4. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses. 4.1.5. The concentration of the MS is analyzed to be within the calibration range for the instrument as established by the concentrations of the standards used for the initial calibration and continuing calibration verifications. 4.1.6. Where applicable, the MS is spiked at the method-specified concentration or at a concentration appropriate for the project data quality objective. 4.2. Evaluation of MS Recovery 4.2.1. The MS spike concentration is compared with the un-spiked, parent-sample concentration. The suitability of the spike concentration is evaluated on a case-by-case basis according to method specifications or project data quality objectives. 4.2.2. MS recovery data is evaluated if specified by applicable data quality objectives, but the recovery of the MS is only associated with the parent sample, unless no LCS is associated with the preparation batch or analytical sequence of 20 samples or less. In this case, the MS recovery is associated with all of the samples in the preparation batch or analytical sequence of 20 samples or less for evaluation of sample data usability. 4.2.3. Where applicable, the control limits for the MS are established by the laboratory for a specified matrix at ± 3X the standard deviation of the mean recovery of the cumulative MS analyses for the associated matrix at a specified concentration. Alternatively, the recovery control limits are established by linear regression for a range of concentrations for the associated matrix using cumulative recovery data. The mean recovery is calculated or regression analysis is applied after outliers are eliminated from the recovery data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also censored from the data set. The mean recovery is recalculated or linear regression is reanalyzed if trend monitoring of individual MS recoveries indicates that a systematic bias has developed and corrective action is needed. 4.2.4. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 4.2.5. If the MS is used to evaluate all associated batch samples, the applicable LCS criteria are used to evaluate the MS recovery. 4.2.6. Where applicable, criteria established by rule, reported method or project data quality objectives are used to evaluate the MS recoveries. 4.2.7. If no other criteria are applicable, the following control limits are used to evaluate the MS recoveries: 80% - 120% (water) for Nitrate, Nitrite, Nitrate + Nitrite, Ammonium, TKN, Total Phosphorus, Orthophosphate 70% - 130% (water) for Metals Laboratory Control Limits

All Organic analytes at any concentration All other analytes amenable to spiking at any concentration All analytes in non-aqueous matrices

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### 4.2.8. When applicable, sample results reported with failed MS recoveries are documented with a valid explanation of the systematic error that occurred. 4.2.9. Analytes or methods technically not amenable to spiking for recovery determinations are not evaluated.
5. 

### Surrogate Spikes - Surrogate spikes are evaluated according to method-specified requirements and the NELAC Quality Systems standards (Chapter 5 and Appendix D). 5.1. The concentration of the surrogate spike is analyzed to be within the initial calibration range established for the instrument. 5.2. When applicable, sample results reported with failed surrogate spike recoveries are documented with a valid explanation of the systematic error that occurred. 5.3. Analytes or methods technically not amenable to spiking for recovery determinations are not evaluated.
6. 

### LCS Duplicates or Replicates (LCSD) and Matrix Spike Duplicates (MSD) - In order of preference, LCSD data are evaluated rather than MSD data, if both types of data are generated for the associated samples. However, the MSD data are also used to evaluate the precision of the analytes for the parent sample and may affect the usability of the parent sample result. 6.1. General Requirements 6.1.1. When applicable, replicate rather than duplicate LCS/MS data are evaluated for precision control. 6.1.2. An LCSD/MSD result is linked or associated with all applicable reported sample results for the same preparation batch or analytical sequence of 20 samples or less. 6.1.3. The LCSD/MSD contains all of the analytes of interest for the method or project, or the mix of analytes in the LCSD is rotated at a routine frequency as suggested in the NELAC Quality Systems standard in Appendix D. 6.1.4. If a preparation step (such as digestion or extraction) is required for the analysis method, the LCSD/MSD is prepared identically with and at the same time as the associated samples in the preparation batch. 6.1.5. The LCSD/MSD is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 6.1.6. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses. 6.1.7. The concentration of the LCSD/MSD is analyzed to be within the calibration range for the instrument as established by the concentrations of the standards used for the initial calibration and continuing calibration verifications. 6.1.8. Where applicable to the evaluation, the MSD spike concentration is compared with the un-spiked, parent-sample concentration. The suitability of the spike concentration is evaluated on a case-by-case basis according to method specifications or project data quality objectives. 6.2. Evaluation of Duplicates 6.2.1. Where applicable, the control limits for the LCSD/MSD are established by the laboratory for a specified matrix at ≤ 3X the standard deviation of the mean precision (absolute or relative) of the cumulative LCSD analyses for the associated matrix at a specified concentration. Alternatively, the precision control limits are established by linear regression for a range of concentrations for the associated matrix using cumulative precision data. The mean precision is calculated or regression analysis is applied after outliers are eliminated from the precision data set using any appropriate

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### 6.2.2. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 6.2.3. Where applicable, criteria established by rule, reported method or project data quality objectives are used to evaluate the LCSD/MSD precision. 6.2.4. If no other criteria are applicable, precision data for the LCSD/MSD are evaluated using the laboratory control limits, with a target precision of ≤ 20% RPD or RSD for water samples and ≤ 40% RPD or RSD for non-aqueous and solid-matrix samples. 6.2.5. When applicable, sample results reported with failed LCSD/MSD precision are documented with a valid explanation of the systematic error that occurred. 6.2.6. Analytes or methods technically not amenable to precision determinations are not evaluated.
7. 

### Sample Duplicates (SD) 7.1. General Requirements 7.1.1. Sample matrix duplicates are evaluated for the analytes present in the sample if no LCSD/MSD data is available or if required by the method or project data quality objectives. 7.1.2. When applicable, replicate rather than duplicate sample data are evaluated for precision control. 7.1.3. If a preparation step (such as digestion or extraction) is required for the analysis method, the SD is prepared identically with and at the same time as the associated samples in the preparation batch. 7.1.4. If no LCSD/MSD is available for evaluation, an SD result is linked or associated with all applicable reported sample results for the same preparation batch or analytical sequence of 20 samples or less. 7.1.5. The SD is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 7.1.6. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses. 7.1.7. The concentration of the SD is analyzed to be within the calibration range for the instrument as established by the concentrations of the standards used for the initial calibration and continuing calibration verifications. 7.2. Evaluation of Sample Duplicates 7.2.1. Where applicable, the control limits for the SD are established by the laboratory for a specified matrix at ≤ 3X the standard deviation of the mean precision (absolute or relative) of the cumulative SD analyses for the associated matrix at a specified concentration. Alternatively, the precision control limits are established by linear regression for a range of concentrations for the associated matrix using cumulative precision data. The mean precision is calculated or regression analysis is applied after outliers are eliminated from the precision dat a set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also censored from the data set. The mean precision is recalculated or linear regression is reanalyzed if trend

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### 7.2.2. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 7.2.3. Where applicable, criteria established by rule, reported method or project data quality objectives are used to evaluate the SD precision. 7.2.4. If no other criteria are applicable, precision data for the SD are evaluated using the laboratory control limits, with a target precision of ≤ 20% RPD or RSD for water samples and ≤ 40% RPD or RSD for non-aqueous and solid-matrix samples. 7.2.5. When applicable, sample results reported with failed SD precision are documented with a valid explanation of the systematic error that occurred. 7.2.6. Analytes or methods technically not amenable to precision determinations are not evaluated.
8. 

### Calibrations - Except as discussed elsewhere in this document, all calibrations are evaluated according to method-specified requirements and applicable NELAC Quality Systems standards. 8.1. Initial Calibration 8.1.1. The number of standard concentrations specified by the method or project data quality objectives is used to perform the initial calibration of the instrument or technique. 8.1.2. Where applicable, the concentration values specified by the method or project data quality objectives are used to perform the initial calibration of the instrument or technique. 8.1.3. If not specified by the method or project objectives and when applicable to the analytical technology, at least two standard concentrations and a blank are used for a linear calibration curve. 8.1.4. The acceptance of the initial calibration is evaluated using the method-specified criteria or the criteria specified by the project data quality objectives. 8.1.5. If not specified by the method or project data quality objectives and when applicable to the analytical technology:

• The correlation coefficient for a regression is ≥ 0.995 when applied to the linear calibration curve.

• Higher-order calibration curve regressions have a coefficient of determination of ≥ 0.99, using 6 calibration points for a second order curve and 7 calibration points for a third order curve. The calibration points used for the curve include a result for the calibration blank. 8.1.6. Where applicable, the initial calibration range is extended by an accepted calibration verification. 8.1.7. Unless allowed by the method or project data quality objectives, applicable sample results whose analyzed concentrations fall outside of the calibration range established by the standard concentrations used for the initial calibration (excluding the calibration blank) and calibration verifications are not usable without further evaluation or qualification. 8.2. Calibration Verification (CV) 8.2.1. At least one verification standard is from a second source as indicated in the applicable NELAC Quality Systems standard for initial calibrations.

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### 8.2.2. An acceptable calibration verification is analyzed at the beginning and end of the analytical batch (verified calibration bracket) or as otherwise required by the applicable NELAC Quality Systems standard for continuing calibration verifications. 8.2.3. The analytical batch is determined as defined by the applicable NELAC Quality Systems standard. 8.2.4. As applicable or when specified by rule or project data quality objectives, the calibration verification standard is analyzed at the method-specified frequency. 8.2.5. As applicable or when specified by rule or project data quality objectives, the calibration verification result is evaluated using the method-specified criteria. 8.2.6. In the absence of acceptance criteria specified by rule, project data quality objectives or method, and where applicable, the control limits for the CV are established by the laboratory for a specified matrix at ± 3X the standard deviation of the mean recovery of the cumulative CV analyses at a specified concentration. For this purpose, recovery is defined as the percent of expected concentration analyzed for the CV standard. The mean recovery is calculated after outliers are eliminated from the recovery data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also censored from the data set. The mean recovery is recalculated if trend monitoring of individual CV recoveries indicates that a systematic bias has developed and corrective action is needed. 8.2.7. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 8.2.8. If a continuing calibration verification did not meet the acceptance criterion, but is immediately reanalyzed with acceptable results, the samples analyzed before the failed verification result are valid, provided that the verification previous to the failed verification and following the reanalyzed verification are also acceptable and comprise a verified calibration bracket or analytical batch. 8.2.9. Sample results that are not analyzed within a verified analytical batch or calibration bracket are reanalyzed or qualified as estimated values. 8.2.10. Alternatively, for purposes of assessing the usability of the sample data where verifications have failed and where the reported sample values are relevant to the regulatory action level, compliance limit or other project data quality objective, the failed continuing calibration verifications are evaluated for potential high or low bias according to the applicable NELAC Quality Systems standards for calibration verifications.
9. 

### Method Blanks or Other Analytical Blanks - All method blanks and other types of analytical blanks use to control analytical contamination are evaluated for the presence of the analytes of interest for the project or method. 9.1. A blank result is linked or associated with all applicable reported sample results for the same preparation batch or analytical sequence of 20 samples or less. 9.2. If a preparation step (such as digestion or extraction) is required for the analysis method, at least one blank is prepared identically with and at the same time as the associated samples in the preparation batch. 9.3. The blank is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 9.4. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses.

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### 9.5. Unless specified otherwise by rule, an applicable analytical method or project data quality objectives, it is expected that the concentration of analytes detected in the blank (above the reported MDL) will be less than 10% of the concentration in the individual samples of the associated preparation batch or analytical sequence (for the affected analytes). 9.6. Where samples are diluted for analysis, the evaluation of the blank results is considered with respect to the dilution factors associated with the samples. 9.7. BOD method blanks are evaluated against the method criterion. 9.8. No colonies are reported in bacteriological method blanks.
10. 

### Field Quality Control Blanks (Trip Blanks, Field Blanks or Equipment Blanks) - Field QC Blanks associated with the samples for a specific sampling event are evaluated for contamination as indicated by the presence of the analytes of interest for the project or method. 10.1. If a preparation step (such as digestion or extraction) is required for the analysis method, the blank is prepared identically as the associated samples in the preparation batch. 10.2. The blank is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 10.3. Unless specified otherwise by rule, an applicable analytical method or project data quality objectives, it is expected that the concentration of analytes detected in the blank (above the reported MDL) will be less than 10% of the concentration in the associated field sample (for the affected analytes). 10.4. Where samples are diluted for analysis, the evaluation of the blank results is considered with respect to the dilution factors associated with the samples.
11. 

### Holding Times - Holding times as specified in the tables in FS 1000 of the DEP SOPs are met. 11.1. For those analytes where there is no separate holding time specified for the extract, digestate or other processed sample, the holding period ends when the sample processing begins. 11.2. Holding times that are specified in “hours” are met if the sample processing (e.g., extraction, digestion, filtration, etc) or analysis, as applicable, begins within the last hour of the specified holding time, accounting for the time zone in which the sample was collected. Example: For a sample with a 24-hour holding time that was collected at 14:15 on May 20, 2007, the sample is within holding time if the sample is processed before 15:15 on May 21, 2007.
11.3. Holding times that are specified in “days” are met if the sample processing begins before 24:00 on the final day, accounting for the time zone in which the sample was collected. 11.4. Clarifications 11.4.1. For microbiology, the holding time is evaluated as the duration between the sample collection date and time and the date and time of the placement of the processed sample into or on the applicable growth medium. 11.4.2. For BOD or CBOD, the holding time is evaluated as the duration between the sample collection date and time and the date and time of the initial DO measurement for the test.

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### 11.4.3. For toxicity testing, the holding time is evaluated as the duration between the sample collection date and time and the date and time of introduction of the last individual test organism into a test sample.
12. 

### Quality Control Check Samples – The following criteria are used to evaluate specific quality control check samples for the indicated test. 12.1. BOD Analyses 12.1.1. For each preparation batch of 20 samples or less, glucose-glutamic acid (GGA) samples are analyzed to obtain values within the method-specified acceptance control limits. 12.1.2. Alternatively, per approved revisions to SM 5210 B, other compounds are used to control the method, when applicable to a specific wastewater effluent. 12.1.3. In order of preference, replicate check sample data are evaluated rather than sample replicate data, if both types of data are generated for the associated samples. 12.1.4. Precision data for the replicate check sample analyses are evaluated using the laboratory control limits, with a target precision of ≤ 20% RPD or RSD expected. 12.1.5. Where applicable, the control limits for the replicate check sample analyses are established by the laboratory at ≤ 3X the standard deviation of the mean precision (absolute or relative) of the cumulative check sample analyses. The mean precision is calculated after outliers are eliminated from the precision data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also censored from the data set. The mean precision is recalculated if trend monitoring of individual check sample results indicates that a systematic error has occurred and corrective action is needed. 12.1.6. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 12.1.7. When applicable, sample results reported with failed check sample precision are documented with a valid explanation of the systematic error that occurred. 12.2. Chlorophyll Analyses 12.2.1. For each preparation batch of 20 samples or less, or at least once every three months, a chlorophyll extract solution in aceton e is analyzed to evaluate recovery at the concentration for the extract. 12.2.2. A vendor-assayed source of chlorophyll is used to prepare the check sample extract. 12.2.3. The concentration of the check sample extract is such that when extrapolated to the volume of a nominal whole-water concentration, the extract appropriately represents the estimated reporting limit for the whole water sample. 12.2.4. The check-sample results at the extract concentration are evaluated using control limits established by the laboratory. 12.2.5. The control limits for the extract check-sample solution are established by thelaboratory at ± 3X the standard deviation of the mean result of the cumulative checksample analyses expressed as a percentage of the expected value of the check-sample concentration. The mean result is calculated after outliers are eliminated from the check-sample data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also cens ored from the data set. The mean result is recalculated if trend monitoring of individual check-sample results indicates that a systematic bias has developed and corrective action is needed.

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### 12.2.6. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 12.3. Matrix-Specific Evaluation for Known or Suspected Interferences - An example of typical interferences of concern to DEP is the analyses for trace metal concentrations in seawater. 12.3.1. When applicable to the project data quality objectives and when technically feasible, known or suspected interferences in the matrix specific to the project analytes and samples are evaluated using quality control (QC) samples of known matrix composition and analyte concentration. 12.3.2. The QC sample result is linked or associated with all applicable reported field sample results for the same preparation batch or analytical sequence of 20 samples or less. 12.3.3. The composition of the matrix for the QC sample is controlled for the analytes and interferences of interest and closely matches or approximates the matrix of the associated field samples. 12.3.4. A naturally derived or artificially formulated matrix is used to prepare the QC sample. 12.3.5. The QC sample is prepared by the analyzing laboratory using a second source standard for the analyte of interest. The second-source standard is selected as indicated in the NELAC Quality Systems standard for initial calibrations. 12.3.6. Alternatively, the QC sample is obtained from an external source such as a commercial vendor of QC-check samples or Standard Reference Materials. 12.3.7. Where applicable, the QC sample is prepared at the method-specified concentration or at a concentration selected according to project data quality objectives. 12.3.8. Where applicable, the concentration of the QC sample is evaluated against any data quality objectives for minimum quantitation level applicable to the data use and does not exceed 2X the minimum quantitation level. 12.3.9. If a preparation step (such as digestion or extraction) is required for the analysis method, the QC sample is prepared identically with and at the same time as the associated samples in the preparation batch. 12.3.10. The QC sample is analyzed or reanalyzed within a verified calibration bracket (analytical sequence). 12.3.11. All reanalyses are documented with a valid explanation of the systematic error that prompted the reanalyses. 12.3.12. The concentration of the QC sample is analyzed to be within the calibration range for the instrument as established by the concentrations of the standards used for the initial calibration and continuing calibration verifications. 12.3.13. As applicable, the recovery for the QC sample is evaluated using methodspecified criteria, vendor-specified criteria, or control limits established by the laboratory. 12.3.14. Where applicable, the control limits for the QC sample are established by the laboratory for a specified matrix at ± 3X the standard deviation of the mean recovery of the cumulative QC sample analyses at a specified concentration. Alternatively, the recovery control limits are established by linear regression for a range of concentrations using cumulative recovery data. The mean recovery is calculated or regression analysis is applied after outliers are eliminated from the recovery data set using any appropriate statistical test for outliers and after outliers due to known systematic errors are also

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### censored from the data set. The mean recovery is recalculated or linear regression is reanalyzed if trend monitoring of individual QC sample recoveries indicates that a systematic bias has developed and corrective action is needed. 12.3.15. Alternatively, control limits are established by the laboratory using other technically justifiable and scientifically sound procedures in accordance with project data quality objectives. 12.3.16. When applicable, sample results reported with failed QC sample recoveries are documented with a valid explanation of the systematic error that occurred.
13. 

### Sample Preservation Checks 13.1. The field records demonstrate positive indication that the samples are properly preserved (including thermal preservation). 13.2. The laboratory records demonstrate that proper thermal preservation was checked upon receipt at the laboratory, per the instructions in the NELAC Quality Systems standards. 13.3. The laboratory records demonstrate that samples that have been collected on the same day and hand-delivered are received at the laboratory in ice if the temperature check fails the applicable rule or method requirement. 13.4. The laboratory records demonstrate positive indication that the samples are checked for proper chemical preservation prior to or during sample preparation or analysis.
14. 

### Evaluation of the Reported MDL 14.1. If no analyte is detected in the evaluated sample, the assessment of sample data usability includes establishing that the laboratory determined, evaluated and verified the reported MDL for the analyte according to the requirements in the applicable NELAC Quality Systems standards, regulatory requirement or reported method. 14.2. The MDL determination, evaluati on and verification are considered when establishing the usability of the sample data where the reported MDL is relevant to the regulatory action level, compliance limit or other project data quality objective. 14.3. For the purposes of data usability evaluation, the DEP-defined MDL is equivalent to the NELAC-defined LOD.
15. 

### Evaluation of the Reported PQL 15.1. If the concentration of the evaluated sample is below the concentration value of the reported PQL or is below the concentration value of the lowest initial calibration standard or continuing calibration verification standard associated with the sample, the assessment of sample data usability includes establishing that the laboratory determined, evaluated and verified the reported PQL for the analyte according to the requirements in the applicable NELAC Quality Systems standards. 15.2. The PQL determination, evaluation and ve rification are considered when establishing the usability of the sample data where the reported PQL is relevant to the regulatory action level, compliance limit or other project data quality objective. 15.3. For the purposes of data usability evaluation, the DEP-defined PQL is equivalent to the NELAC-defined LOQ.
16. 

### Evaluation of Reversals (Parts vs. Whole Comparison) 16.1. Where applicable, sample results are evaluated to determine if the sum of reported parts or fractions for the associated sample analyte results exceed 120% of the corresponding reported or calculated whole.

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### 16.2. Evaluation of reversals with respect to sample data usability is assessed on a caseby-case basis with more extensive validation of the specifically affected analyses conducted as needed. Examples of reversals include the following:
Parameter Part Parameter Whole
Total ammonia TKN Orthophosphate Total phosphorus
Nitrate Measured Total nitrite/nitrate
Nitrite Measured Total nitrite/nitrate
Sum of nitrite and nitrate Measured Total nitrite/nitrate Sum of nitrite, nitrate and TKN Measured Total nitrogen
Sum of measured total nitrite/nitrate and TKN Measured Total nitrogen

Filtered sample results (e.g., dissolved metals) Unfiltered sample results (e.g., total metals) Methyl mercury Total mercury
DOC TOC

17. 

### General Principles of Data Quality Assessment - The following principles will be used when evaluating sample data for data usability determinations using specified Data Quality Indicators. The guidelines discussed in this section are only applicable if specific data quality assessment directives have not been provided in any other Department Rule or reporting format. The examples given below are illustrative and simplistic in nature. 17.1. Supporting data that fail the target acceptance criteria for specific Data Quality Indicators will be evaluated against the affected sample result and the magnitude of the failure. Examples: A wastewater effluent, with a nitrate discharge permit level of 10 mg/L, was analyzed by a laboratory and found to have a nitrate concentration of 0.26 mg/L. For the recovery of the Laboratory Control Sample (LCS), the laboratory has established acceptance criteria of 80 – 120%. For this particular batch of samples, recovery for the LCS was 78%, and the laboratory properly qualified the result with a “J”. Because the actual nitrate value was significantly lower than the permit limit, and the quality control failure was relatively minor, this result is judged to be usable. A Class I waterbody was sampled for benzene to determine if concentrations complied with the water quality standard of 1.18 ug/L. A benzene analysis was performed with initial calibration standards ranging from 1 ug/L to 200 ug/L. All QC checks associated with this analysis were within the calibration range and within acceptance criteria. One of the project samples had a benzene concentration of 210 ug/L. The sample was qualified by the laboratory with a “J” and an explanation provided that the result exceeded the high level calibration standard. Because of the absolute magnitude of the

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### sample result (exceeding the water quality standard by nearly 209 ug/L), and the fact that it exceeded the high level standard by only 5%, the data is usable for the project. A wastewater effluent sample, which was collected to determine compliance with a 10 ug/L arsenic permit limit, was found to have 9.5 ug/L of arsenic. While the Laboratory Control Sample acceptance criteria previously established by the laboratory was 75 – 125%, the LCS recovery associated with this sample was only 65%. These data are unusable to demonstrate compliance, due to the close proximity of the sample to the action level, and the low LCS recovery (well outside of the targeted acceptance criteria).
17.2. The magnitude of the sample result is considered when evaluating the consequences of the failed or absent Data Quality Indicators. Examples: An Everglades surface water sample, analyzed for compliance with a 10 ug/L total phosphorus criterion, was found to contain 21 ug/l of total phosphorus. The sample was properly preserved, but due to a laboratory mistake, the original sample had to be re-analyzed. The second analysis produced a total phosphorus result of 17 ug/L, but now the holding time was exceeded by 1 week. The value was qualified by the laboratory with a “Q” for exceeding the holding time. Despite a reduction in the phosphorus concentration that could have been associated with exceeding the holding time, the analytical result still exceeded the action level, and the datum is usable. For a waste facility study, benzene was found in groundwater at a concentration of 5.7 ug/L. However, the reported method detection limit (MDL) for this particular benzene analysis was 1 ug/L, with a practical quantitation limit (PQL) of 4 ug/L. The Rule 62-777 FAC guidance document for routinely achievable PQLs indicates the target PQL for benzene is 1 ug/L. In this case, despite the elevated detection limit (indicating use of a less sensitive method), the sample result was above the reported PQL, and this result is usable. A surface water sample was analyzed by membrane filtration for fecal coliform, to determine if the 800 Colonies/100 mL water quality standard was exceeded. The analytical result was calculated to be 1,200 Colonies/100 mL. Method blanks were run simultaneously with the surface water samples by filtering 100 mL of sterilized dilution water, which resulted in the presence of 2 – 5 target colonies in the blank samples. The laboratory did not use “V” (analyte detected in blank) to qualify the result. This result is usable despite the failure of the lab to properly qualify the samples, the high magnitude of the result exceedance, compared with the minor contamination in the blanks. Biochemical Oxygen Demand analysis conducted on a surface water sample yielded a result of 5.5 mg/L. Dissolved oxygen depletion in the associated method blank was 0.3 mg/L, exceeding method criteria by 0.1 mg/L. The results were properly qualified with “V”. The data is usable based on the magnitude of the exceedance compared with the quality control failure.
17.3. The evaluation of laboratory performance or the determination of usability for a data set will be based on a preponderance of Data Quality Indicator failures pointing to specific, systematic problems with the laboratory operation or the data set. Examples:

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### The analytical data generated by a County surface water monitoring program were being audited to determine if the data could support Impaired Waters Rule listing decisions for the Total Maximum Daily Load (TMDL) program. Fifteen chlorophyll a samples, which had been analyzed over a five year period, were evaluated. Twice in this five year period, due to laboratory oversights, the 48 hour hold time for filtration was not met. Records demonstrated that the 48 hour hold time was the laboratory’s target and that a quality system for meeting this objective was evident. Upon reviewing additional chlorophyll a quality control records, it was determined these holding time exceedances were isolated instances and that the 5 year period of data is usable. Upon auditing a laboratory’s quality control information associated with data submitted to the TMDL program, it was discovered that the majority of samples were analyzed beyond the accepted holding time, and that none of these results were qualified with “Q”. When the laboratory manager was asked about the discrepancy, they indicated that the qualifiers were purposely suppressed so that the TMDL program would use all the data. Because these admissions indicated that the entire quality system was compromised, the data were deemed not usable.
17.4. Project management goals are taken into consideration when making data usability assessments. Specific Data Quality Objectives are established for certain analytical activities within a project, and these objectives will be evaluated when making a usability statement. Examples: A cleanup goal established for a waste remediation site stated that all analytical work must be sensitive enough to meet the Cleanup Target Levels (CTL) in Rule 62 – 777 FAC. During the project, a previously unknown area of contamination was discovered, and the Project Manager ordered some grab samples to delineate the aerial extent and magnitude of the newly found contamination. The lab used a method with a higher PQL (that did not meetthe original cleanup objectives) but use of this method allowed a rapid turnaround time, enabling an effective adaptive management approach to better address the newly discovered contamination. These data are usable based on this set of objectives (delineating the new area of concern). Four monitoring wells were sampled for trichloroethane (TCE) at a groundwater remediation site previously known to have TCE concentrations ranging from 20-30 ug/L. During this particular sampling, TCE measured at the four wells was found to be below 1 ug/L, and there were no laboratory quality control failures of any kind. However, sampling records indicated that large volumes of groundwater were purged with a centrifugal pump in a short time period, and that dissolved oxygen levels in the wells exceed 7 mg/L. Because the evidence indicates that improper sampling occurred, resulting in excessive aeration and de-gassing of volatile compounds, these data were determined to be unusable.
18. 

### Procedure for Data Usability Determinations 18.1. Data auditors will review and evaluate the following information:

• The purpose for auditing the data;

• The reported values of the analytical results;

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### 16 of 16 Revision Date: March 31, 2008 

### • The relationship between each analytical result and an associated decision or action level;

• The documented calibration, quality control and other supporting data compared with designated Data Quality Indicators in 62-160, F.A.C. or other specified Data Quality Objectives;

• The pattern, frequency, and magnitude of any failures or other deficiencies associated with the results;

• The extent to which the audited data set fulfills the Data Quality Objectives of the project or Program. 18.2. Based on the evaluation, the auditors will determine how the data can be used by the relevant Department programs.
19. 

### Summary of the Data Usability Assessment Process Based on the above inputs, the auditor will extrapolate audit findings to determine the overall performance of a laboratory for a period of record, and determine the usability of the data in question for a Department purpose. Usability assessments will evaluate the Data Quality Indicator results for the subject data set relative to DEP program or project objectives, and the follow the principles characterized in this guidance document to draw an “overall conclusion” concerning the usability of the data set. This conclusion will address individual samples or will express a “general assessment” bas ed on examination of a representative sample set over a pre-determined time period, depending on the project objectives and the sample set being evaluated. This assessment will include, where app licable, the evaluation of data trends relative to laboratory corrective actions or laboratory events (e.g., laboratory instituted NELAC standards in November of 2004). The resulting usability determination would be characterized over certain segments of the audited period. Recommendations concerning usability of the data will be communicated with appropriate Department staff.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A3 -1 

## Appendix A 3 . Data Supporting EPA’s Reference Approach for Deriving Numeric Nutrient Criteria for Florida Streams

Appendix A3 contains the data EPA used to derive numeric nutrient criteria for Florida streams. It is being provided in electronic form on CD. A copy of the CD can be obtained by contacting EPA’s docket office. The docket identification number is EPA- HQ

- OW -2009-0596.
The Office of Water (OW) Docket Center is open from 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding legal holidays. The OW Docket Center telephone number is 202-566-1744 and the Docket address is OW Docket, EPA Wes t, Room 3334, 1301 Constitution Ave., NW, Washington, DC 20004.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -1 

## Appendix A4. FDEP’s Method for Calculating a Corridor

- based Landscape Development Intensity Index (LDI)

LDI method for Streams February 2008 FDEP

In your view have the following layers to start with:

**•** Site point coverage

**•** FL NHD (24K)

o Lines

o Polygons (waterbodies as well as areas)

General guidelines: The LDI is calculated using the land use within a 100 m buffer around the stream, 10,000m (10 km ) upstream from the site.
In order to do this, follow these steps:
1. Start with the site of your choice 2. Draw a circle with a 10,000 m radius using the site as the center point. 3. Select the 24K NHD linework upstream of the site until you reach the circle bound ary
a. If there is not 10,000 m of stream upstream of the point, select the linework upstream of the point until you run out of stream. 4. Create a 100 m buffer around the linework 5. Cut the buffer polygon so that there is no buffer downstream of the site 6. Select the 24K NHD polygons (waterbodies and areas) that are upstream of the site, these represent wide spots in the stream/river. 7. Create 100 m buffer around the polygons 8. Append the buffers from the streamline and the polygons to create one buffer shapefile 9. Disso lve the buffers to eliminate overlap 10. Erase the polygons from the buffers (so that the buffer goes around the wide spots in the streams, wide spots in the streams are not to be included in the buffer)

a. Water has an LDI score of 1 and can artificially lower the score if the water from the stream is included in the calculation of the LDI for that stream. Water that falls within the 100 m buffer but is not in the streamline can be counted in the calculation. b. If the NHD waterbodies remain selected, ArcGIS will on ly erase the selected polygons 11. Now you are ready to clip the corresponding WMD land use to the buffer.

a. Always use the most recent land use available 12. Recalculate the area to make sure accurate areas are available ( I always recalculate to Acres)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -2 13. Bring the s hapefile dbase file and LDI_by_LU3.xls into Excel 14. Create pivot table from data

a. Click data menu b. Select “pivot table and pivot chart report”

c. Click next on the first 2 screens d. Click finish on the next screen

e. Drag and drop “Level 3” landuse codes into “Drop ro w fields here” f. Drag and drop the recalculated area field into “Drop data items here” 15. Copy the columns that the pivot table created 16. Paste Special

- > Values

a. You cannot manipulate data while it is in a pivot table 17. Add your column headings in (Level 3, Acres) 18. Add the following headings: Percent LDI Coefficient, LDI value 19. Calculate the percent by dividing the acres for one Level 3 value by the total acres (calculated by the pivot table) 20. Populate the LDI coefficient field

a. Click on fx (add function) b. Choose VLOOKUP i. Lookup value = Level 3 value (ex. 1100) ii. Table array = LDI Table (see next page for LDI values for each FLUCC code), highlight Level 3 and LDI columns iii. Col_index_number = 2 (the column that the LDI values are stored in) iv. Range_lookup = false (so it will only return exact matches) 21. Calculate the LDI value by multiplying the percent by the LDI coefficient 22. Add individual LDI values for total LDI value. 23. Record total LDI value and land use used (year and district)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -3 LDI Codes for Each FLUCC code

FLUCC Description LDI 1000 Urban and Built

- up 7.39 1009 Mobile Home Units Any Density 6.79 1100 Residential, Low Density <Less than two dwelling units per acre> 6.79 1110 Low Density Residential - Fixed Single Family Units 6.79 1120 Low Density Residential - Mobile Home Units 6.79 1130 Low Density Residential - Mixed Units <Fixed and mobile home units> 6.79 1140 Ranchettes - Fixed Single Family Units 6.79 1150 Ranchettes - Mobile Units 6.79 1160 Ranchettes - Mixed Units 6.79 1190 Low Density Under Construction 6.79 1200 Residential, Medium Density <Two - five dwelling units per acre> 7.59 1210 Medium Density Residential - Fixed Single Family Units 7.59 1220 Medium Density Residential - Mobile Home Units 7.59 1230 Medium Density Residential - Mixed Units <Fixed and mobile home units> 7.59 1290 Medium Density Under Construction 7.59 1300 Residential, High Density 8.66 1310 High Density Residential - Fixed Single Family Units <Si or more 7.99 1320 High Density Residential - Mobile Home Units <Si or more 7.99 1330 Multiple Dwelling Units - Low Rise <Two stories or less> 8.66 1340 Multiple Dwelling Units - High Rise <Three stories or more> 9.19 1350 High Density Residential - Mixed Units <Fixed and mobile home units> 7.99 1390 High Density Under Construction 7.99 1400 Commercial and Services 8.00 1410 Retail Sales and Services 8.00 1411 Shopping Center 9.18 1420 Wholesale Sales and Services <excluding warehouses associated with 8.00 1423 JUNK YARD 9.18 1424 FARMERS MARKET 8.00 1430 Professional Services 8.00 1440 Cultural and Entertainment 8.07 1443 OPEN AIR THEATER 8.07 1450 Tourist Services 8.00 1452 MOTEL 8.00 1453 TRAVEL TRAILER PARK 8.00 1454 CAMPGROUND 4.09 1460 Oil and Gas Storage 8.00 1470 Mixed Commercial and Services 9.42 1480 Cemeteries 4.09 1490 Commercial and Services Under Construction 8.00 1500 Industrial 8.32 1510 Food Processing 8.32 1513 SEAFOOD PROCESSING 8.32 1514 MEAT PACKING FACILITY 8.32 1515 POULTRY AND/OR EGG PROCESSING 8.32 1516 GRAIN AND LEGUME PROCESSING 8.32Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -4 FLUCC Description LDI 1520 Timber Processing 8.32 1521 SAWMILL 8.32 1522 PLYWOOD AND VENEER MILL 8.32 1523 PULP AND PAPER MILL 8.32 1526 LOG HOME PREFABRICATION 8.32 1527 WOODYARD 8.32 1530 Mineral Processing 8.32 1532 PHOSPHATE PROCESSING 8.32 1533 LIMEROCK PROCESSING 8.32 1535 HEAVY MINERALS PROCESSING 8.32 1540 Oil and Gas Processing 8.32 1544 LIQUIFIED GASES 8.32 1545 ASPHALT PLANT 8.32 1550 Other Light Industrial 8.32 1551 BOAT BUILDING AND REPAIR 8.32 1552 ELECTRONICS 8.32 1554 AIRCRAFT BUILDING AND REPAIR 8.32 1556 MOBILE HOME MANUFACTURER 8.32 1560 Other Heavy Industrial 8.32 1561 Ship Building and Repair 8.32 1562 Prestressed Concrete Plants 8.32 1564 CEMENT PLANT 8.32 1565 PLASTIC PIPE PLANT 8.32 1570 Chemical Processing Plants 8.32 1580 ??? 8.32 1590 Industrial Under Construction 8.32 1600 Extractive 8.32 1610 Strip Mines 8.32 1611 Clays 8.32 1612 Peat 8.32 1613 HEAVY MINERAL MINE 8.32 1614 PHOSPHATE MINE 8.32 1620 Sand and Gravel Pits 8.32 1630 Rock Quarries 8.32 1631 LIMEROCK QUARRY 8.32 1632 DOLOMITE QUARRY 8.32 1633 PHOSPHATE 8.32 1640 Oil and Gas Fields 8.32 1650 Reclaimed Land 8.32 1660 Holding Ponds 8.32 1670 Inactive Strip Mines/Rock Quarries or holding ponds 8.32 1700 Institutional 8.07 1710 Educational Facilities 8.07 1720 Religious 8.07 1730 Military 8.07 1736 NATIONAL GUARD INSTALLATION 8.07 1740 Medical and Health Care 8.07Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -5 FLUCC Description LDI 1741 HOSPITIAL 8.07 1742 NURSING HOME 8.07 1750 Governmental 8.07 1756 MAINTENANCE YARD 8.07 1760 Correctional Facilities 8.07 1761 STATE PRISON 8.07 1765 MUNICIPAL PRISON 8.07 1770 Other Institutional Facilities 8.07 1780 Commercial Child Care 8.07 1790 Institutional Under Construction 8.07 1800 Recreational 4.09 1810 Swimming Beach 4.09 1820 Golf Courses 6.92 1830 Race Tracks 6.92 1831 AUTOMOBILE RACING TRACK 6.92 1832 HORSE RACING TRACK 6.92 1833 DOG RACING TRACK 6.92 1840 Marinas and Fish Camps 6.92 1850 Parks and Zoos 4.09 1851 CITY PARK 4.09 1852 ZOO 6.92 1860 Community Recreational Facilities 4.09 1870 Stadiums 6.92 1880 Historical Sites 8.07 1890 Under Construction or Other Recreational Facilities 4.09 1900 Open Land 1.85 1910 Undeveloped Urban Land 1.85 1920 Inactive Development Land 1.85 1923 INACTIVE DEVELOPMENT LAND NONFORESTED 1.85 1924 INACTIVE DEVELOPMENT LAND FORESTED 1.85 1930 Urban Land in Transition Without Positive Indicators of Intended Activity 1.85 1940 Other Open Land 1.85 2000 Agriculture 3.88 2100 Pastures and Fields 3.51 2110 Improved Pastures 3.51 2120 Unimproved Pastures 2.06 2130 Woodland Pastures 2.06 2140 Row Crops 4.63 2141 Potatoes and Cabbage 4.63 2150 Field Crops 4.63 2156 Field Crops - Sugar Cane 4.63 2160 Mixed Crops 4.63 2200 Tree Crops 4.06 2210 Citrus Groves 4.06 2220 Fruit Orchards 4.06 2221 PEACHES 4.06 2224 BLUEBERRIES 4.06Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -6 FLUCC Description LDI 2230 Other Groves 4.06 2231 PECANS 4.06 2240 Abandoned Tree Crops 4.06 2300 Feeding Operations 5.15 2310 Cattle Feeding Operations 5.15 2320 Poultry Feeding Operations 5.15 2330 Swine Feeding Operations 5.15 2400 Nurseries and Vineyards 4.06 2410 Tree Nurseries 4.06 2420 Sod Farms 4.06 2430 Ornamental Nurseries 4.06 2431 Shade Ferns 4.06 2432 Hammonck Ferns 4.06 2440 Vineyards 4.06 2450 Floriculture 4.06 2460 Timber Nursery 4.06 2500 Specialty Farms 4.06 2510 Horse Farms 5.15 2520 Dairies 5.15 2530 Kennels 5.15 2540 Aquaculture 5.15 2550 Tropical Fish Farms 5.15 2590 Other Specialty Farms 5.15 2600 Other Open Lands 2.06 2610 Fallow Cropland 2.06 2620 Old Field 2.06 3000 Rangeland 2.06 3100 Herbaceous 2.06 3200 Shrub and Brushland 2.06 3210 Palmetto Prairies 2.06 3220 Coastal Scrub 2.06 3290 Other Shrubs and Brush 2.06 3300 Mixed Rangeland 2.06 4000 Upland Forests 1.00 4100 Upland Coniferous Forests 1.00 4110 Pine Flatwoods or Mesic Flatwoods 1.00 4119 Pine Flatwoods - Melaleuca Infested 1.00 4120 Longleaf Pine

- Xeric Oak or Longleaf Sandhill 1.00 4130 Sand Pine or Sand Pine Scrub 1.00 4140 Pine - Mesic Oak 1.00 4190 Hunting Plantation Woodlands 1.58 4200 Upland Hardwood Forests 1.00 4210 Oak Sandhill 1.00 4220 Brazilian Pepper 1.00 4230 Oak - Pine - Hickory 1.00 4240 Melaleuca 1.00 4250 Temperate Hardwood 1.00Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -7 FLUCC Description LDI 4260 Tropical Hardwoods 1.00 4270 Live Oak 1.00 4280 Cabbage Palm 1.00 4290 Wax Myrtle - Willow 1.00 4300 Upland Hardwood Forests Continued 1.00 4310 Beech - Magnolia 1.00 4320 Oak Scrub 1.00 4330 Western Everglades Hardwoods 1.00 4340 Hardwood - Conifer Mixed 1.00 4350 Dead Trees 1.00 4370 Australian Pine 1.00 4380 Mixed Hardwoods 1.00 4390 Maritime Hammock 1.00 4400 Tree Plantations 1.58 4410 Pine Plantations 1.58 4420 Hardwood Plantations 1.58 4430 Forest Regeneration 1.58 4440 Experimental Tree Plots 1.58 4450 Seed Tree Plantations 1.58 5000 Water 1.00 5100 Streams and Waterways 1.00 5200 Lakes 1.00 5210 Lakes Larger Than 500 Acres (202 Hectares) 1.00 5220 Lakes Larger Than 100 Acres (40 Hectares), but Less Than 500 Acres 1.00 5230 Lakes Larger Than 10 Acres (4 Hectares), but Less Than 100 Acres 1.00 5240 Lakes Less Than 10 Acres (4 hectares) Which are Dominant Features 1.00 5300 Reservoirs 4.09 5310 Reservoirs Larger Than 500 Acres (202 Hectares) 4.09 5320 Reservoirs Larger Than 100 Acres (40 Hectares), but Less Than 500 Acres 4.09 5330 Reservoirs Larger Than 10 Acres (4 Hectares), but Less Than 100 Acres 4.09 5340 Reservoirs less than 10 Acres (4 Hectares) which are dominant features 4.09 5400 Bays and Estuaries 1.00 5410 Embayments Opening Directly into the Gulf of Mexico or the Atlantic Ocean 1.00 5420 Embayments Not Opening Directly into the Gulf of Mexico or the Atlantic Ocean 1.00 5500 Major Springs 1.00 5600 Slough Waters 1.00 6000 Wetlands 1.00 6100 Wetland Hardwood Forests 1.00 6110 Bay Swamps 1.00 6120 Mangrove Swamps 1.00 6130 Gum Swamps 1.00 6140 Shrub Swamps 1.00 6150 Bottomland Hardwood Forest 1.00 6160 Inland Ponds and Sloughs 1.00 6170 Mixed Wetland Hardwoods 1.00 6171 Mixed Wetland Hardwoods - Willows 1.00 6172 Mixed Wetland Hardwoods - Mixed Shrubs 1.00Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -8 FLUCC Description LDI 6180 Cabbage Palm Savanna 1.00 6200 Wetland Coniferous Forests 1.00 6210 Cypress 1.00 6218 Cypress - Melaleuca Infested 1.00 6219 Cypress - with Wet Prairies 1.00 6220 Wet Flatwoods 1.00 6230 Atlantic White Cedar 1.00 6240 Cypress - Pine - Cabbage Palm 1.00 6300 R Wetland Mixed Forest 1.00 6310 Hydric Hammock 1.00 6320 Tidal Swamp 1.00 6400 Vegetated Non

- forested Wetlands 1.00 6410 Freshwater Marshes 1.00 6411 Freshwater Marshes - Sawgrass 1.00 6412 Freshwater Marshes - Cattail 1.00 6420 Salt marshes 1.00 6430 Wet Prairies 1.00 6439 Wet Prairies - with Pine 1.00 6440 Emergent Aquatic Vegetation 1.00 6450 Submergent Aquatic Vegetation 1.00 6451 Hydrilla 1.00 6460 Mixed Scrub

- Shrub Wetland 1.00 6500 Non

- vegetated 1.00 6510 Salt Barrens 1.00 6520 Intertidal Areas 1.00 6530 Inland Shores/Ephemeral Ponds 1.00 6540 Oyster Bars 1.00 6600 Cut over Wetlands 1.58 6900 Wetland Scrub Scrub 1.00 7000 Barren Land 1.00 7100 Beaches 1.00 7200 Sand Other Than Beaches 1.00 7300 Eposed Rock 1.00 7310 Eposed Rock with Marsh Grasses 1.00 7400 Disturbed Lands 4.09 7410 Rural Land in Transition Without Positive Indicators of Intended Activity 4.09 7420 Borrow Areas 4.09 7430 Spoil Areas 4.09 7440 Fill Areas 4.09 7450 Burned Areas 1.00 7500 Riverine Sandbars 1.00 8000 Transportation, Communication and Utilities 8.05 8100 Transportation 7.81 8110 Airports 8.28 8111 Commercial Airport 8.28 8112 GENERAL AVIATION 8.28 8113 PRIVATE AIRPORT 8.28Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A4 -9 FLUCC Description LDI 8120 Railroads 7.81 8130 Bus and Truck Terminals 8.28 8132 BUS TERMINAL 8.28 8133 TRUCK TERMINAL 8.28 8140 Roads and Highways 8.28 8141 LIMITED ACCESS HIGHWAY (INTERSTATE) 8.28 8142 DIVIDED HIGHWAY (FEDERAL

- STATE) 8.28 8143 TWO LANE HIGHWAY 7.81 8147 TRANSPORTATION CORRIDOR 7.81 8150 Port Facilities 8.28 8160 Canals and Locks 8.28 8170 Oil, Water, or Gas Long Distance Transmission Line 8.28 8180 Auto Parking Facilities (Highway Rest Areas) 8.28 8190 Transportation Facilities Under Construction 8.28 8191 Highways 8.28 8192 Railroads 8.28 8200 Communications 8.32 8210 Transmission Towers 8.32 8220 Communication Facilities 8.32 8290 Communication Facilities Under Construction 8.32 8300 Utilities 8.32 8310 Electrical Power Facilities 10.00 8311 THERMAL (COAL

- FIRED) ELECTRICAL POWER GENERATING PLANT 10.00 8315 ELECTRICAL POWER SUBSTATION 10.00 8320 Electrical Power Transmission Lines 1.85 8330 Water Supply Plants 8.32 8340 Sewage Treatment 8.32 8350 Solid Waste Disposal 8.32 8390 Utilities Under Construction 8.32 9000 Special Classifications 1.00 9100 Vegetative 1.00 9110 Sea Grass 1.00Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -1 

## Appendix A5. FDEP’s Stream Benchmark Summaries

Cross

- references:

FDEP’s Stream Benchmark Summaries Appendix B-17: FDEP’s Stream Benchmark Summaries . U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters

. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule. January 14, 2010 . EPA

- HQ

- OW -2009-0596, FRL-9105-1.
Appendix 7-A . U.S. EPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters. United States Environmental Protection Agency, Office of Water, Office of Science and Technology, Health and Ecological Criteria Division, Washington, DC.

And,
FDEP’s Site Information and Taxa Lists for Stream Benchmark Site Samples Appendix B-15: FDEP’s Site Information and Taxa Lists for Stream Benchmark Site Samples
, U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters

. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule. January 14, 2010 . EPA

- HQ

- OW

2009-0596, FRL-9105-1.
Appendix 3-D . USEPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters. United States Environmental Protection Agency, Office of Water, Office of Science and Technology, Health and Ecological Criteria Division, Washington, DC.

# FDEP’s Stre am Benchmark Summaries

(Appendix 7-A from USEPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters . United States Environm ental Protection Agency, Office of Water, Office of Science and Technol ogy, Health and Ecological Criteria Division, Washington, DC.)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -3 BONE VALLEY NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Deer Prairie Creek STORET Station 27051558216422

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.57, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of agriculture and residential land use observed beyond an extensive forested riparian buffer zone. The majority of the watershed lies within conservation lands. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Deer Prairie Creek (Station 27051558216422) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Deer Prairie Creek (Station 27051558216422) showing close-up of sampling site.

Photograph of Deer Prairie CreekTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -4 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 117 Hydrologic Score 2 LDI Score 1.57 SCI Score 74 A Total Phosphorus Geometric Mean (mg/L) 0.285 Total Nitrogen Geometric Mean (mg/L) 1.14

The habitat assessment sc ored in the high end of the “Suboptimal” range due to the low water levels. The Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, fully supporting the Class III designatio n.
Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 89.00 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 10.67 Number of Taxa 51

Minimal periphyton growth was observed at the site, which would al low for optimal substrate use by benthic invertebrate and fish communities. Of the 51 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclu sion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Deer Prairie Creek. Based on our an alyses, the Department concluded th at the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -5 BONE VALLEY NUTRIENT REGION

Nutrient Benchmark Site Summary



## East Fork Manatee River STORET Station 273116508208152

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 2.22, suggesting relatively benign human influences in the watershed. During the site visit , aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with field/pasture located beyond a riparian buffer zone. Although there is phosphate mining in the area, no phosphate pro cess water is discharged to the East fork of the Manatee River. The site is located within protected conservation lands.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of East Fork Manatee River (Station 273116508208152) showing watershed vi cinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of East Fork Manatee River (Station 273116508208152) showing close-up of sampling site.

Photograph of East Fork Manatee RiverTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -6 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 2 LDI Score 2.22 SCI Score 71 A Total Phosphorus Geometric Mean (mg/L) 0.417 Total Nitrogen Geometric Mean (mg/L) 1.04

The habitat assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III designation.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 76.00 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 23.67 Number of Taxa 57

Of the 57 algal taxa observed, the majority of the periphyton community was composed of diatoms. Algal growth was very sparse throughout the strea m, and no nuisance algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) a t East Fork Manatee River. Based on our an alyses, the Department concluded that the site was influenced only by low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentra tions reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -7 BONE VALLEY N UTRIENT REGION

Nutrient Benchmark Site Summary 

## Manatee River @ SR 64 STORET Station 24010002

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.46, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with some field/pasture beyond an extensive riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Manatee River at SR 64 (Station 24010002) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Manatee River at SR 64 (Station 24010002) showing close-up of sampling site.

Photograph of Manatee River at SR 64Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -8 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 109 Hydrologic Score 2 LDI Score 1.46 SC I Score 64 A Total Phosphorus Geometric Mean (mg/L) 0.560 Total Nitrogen Geometric Mean (mg/L) 0.67

The habitat assessment sc ored in the “Suboptimal” range, mostly due to limited in

- stream substrate diversity and availability. Despite this, the Stream Condition Index scored in the “Healthy” category, indicating a diverse assemblage of sensitive invertebrates, fully supporting the Class III designation.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 90.00 % Chlorophycota 0 % Cryptophycophyta 0 % Cyanophycota 9.33 Number of Taxa 34

Of the 34 algal taxa observed, the majority of the periphyton community was composed of diatoms. This site has been a DEP reference stream for 17 years, and local DEP biolog ists have sampled this site many times during that period. However, during the December, 17, 2008 sampling event, a significant amount of filamentous algal growth was observed. Peggy Morgan (personal communication) stated that while these types of blooms are common in the region during low water events, algal growth is routinely sparse at this location. There have been no additional adverse human activities or changes in watershed land use during the past 17 year period, therefore it is likely that this type of algal growth is an infrequent natural condition, and that the magnitude, frequency and duration of the growth events are limited, as evidenced by the healthy invertebrate community. Ms. Morgan returned to this area of the Manatee River on March 2 , 2009, and reported that the algae observed in December was significantly reduced, and that algal coverage had returned to typical (low coverage) levels. This suggests that even in minimally disturbed streams with naturally elevated phosphorus levels, sp oradic algal growth occurs, however the magnitude, frequency and duration of the growth events are limited.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrolo gy, local land use, and algal and macroinvertebrate communities) at Manatee River at SR 64. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it maintained healthy biol ogical assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated



### use (propagation and maintenance of a healthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -9 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary



## Alapaha River @ CR 150 STORET Station 21010008

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.81, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the watershed land use was predominately forest/natural , with some agricultural activities occurring beyond an extensive riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Alapaha River (Station 21020008) showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Alapaha River at CR 150 showing close-up of sampling site

Photograph of the Alapaha River at CR 150Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -10 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 138 Hydrologic Score 1 LDI Score 1.81 SCI Score 59 A Total Phosphorus Geometric Mean (mg/L) 0.309 Total Nitrogen Geometric Mean (mg/L) 2.07

The Habitat A ssessment sc ore was in the “Optimal” range. The SCI scored in the “Healthy” range, indicating a diverse community of aquatic invertebrates, and demonstrating that this stream is meeting its Class III designated

use.
Periphyton Community Composition

Algal Communi ty Structure % Composition % Bacillariophyta 83.44 % Chlorophycota 2.92 % Cryptophycophyta 0 % Cyanophycota 13.31 Number of Taxa 62

Minimal to moderate periphyton growth was observed at this site , and there were no nuisance filamentous algae. Of the 62 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land u se, and macroinvertebrate and algal communities) at the Alapaha River at CR 150. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biologic al assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -11 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Suwannee River @ White Springs, FL (US 41) STORET Station 21010040 / SUW040C1

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Int ensity Index score of 1.27, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watersh ed land use was forest/natural with silviculture beyond an extensive forested riparian buffer zone. The majority of the water emanates from the Okeefenokee National Wildlife Refuge. There are no point source discharges upstream of the site.

Image Remove d Due to Copyright. DOQQ 2004 aerial photograph of the Suwannee River @ US 41 showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Suwannee River @ US 41 showing close-up of sampling site.

Photograph of the Suwannee River at US Hwy 41Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -12 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 145 Hydrologic Score 2 LDI Score 1.27 SCI Score 69 A Total Phosphorus Geometric Mean (mg/L) 0.154 Total Nitrogen Geometric Mean ( mg/L) 1.24

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, exhibiting a diverse assemblage of sensitive aquatic invertebrates and indicating the site is definitively meeting the Class III designa ted use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 54.43 % Chlorophycota 8.20 % Cryptophycophyta 0 % Cyanophycota 37.38 Number of Taxa 66

Very little periphyton was observed at this site, and there was no nuisance algal growth. Of the 66 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Suwannee River at US Highway 41. Based on

our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors an d that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -13 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Deep Creek @ US 441 STORET Station DEP010C1

Land Use
This station was considered to be a candidate reference site based on the Landscape Dev elopment Intensity Index score of 1.34, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some minimal silviculture. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Deep Creek @ US 441 (Station DEP010C1) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Deep Creek @ US 441 (Station DEP010C1) showing close-up of sampling site.

Photograph of Deep Creek @ US 441Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -14 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 97 Hydrologic Score 3 LDI Score 1.34 SCI Score 80 A Total Phosphorus Geometric Mean (mg/L) 0.112 Total Nitrogen Geometric Mean (mg/L) 1.04

The Habitat A ssessment sc ore was in the “Suboptimal” range, predominantly due to low water velocity (drought conditions) and the presence of naturally eroding sandy banks. The SCI score was in the “Exceptional ” category, exhibiting an unusually diverse assemblage of sensitive invertebrates and indicating the site is definitely meeting its Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 93.33 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 6.33 Number of Taxa 58

Very little periphyton was obse rved at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 58 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Deep Creek @ US 441. Based on our an alyses, the Department concluded that the si te was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -15 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Falling Creek @ C

- 131 STORET Station FAL020C1

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.36, suggesting benign human influences in the watershed. Before and during the site visit, aerial photogr aphs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and field/pasture located beyond an extensive forested riparian b uffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Falling Creek @ C-131 (Station FAL020C1) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2 004 aerial photograph of Falling Creek @ C-131 (Station FAL020C1) showing close -up of sampling site.

Photograph of Falling Creek @ C-131Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -16 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 142 Hydrologic Score 3 LDI Sc ore 1.36 SCI Score 55 A Total Phosphorus Geometric Mean (mg/L) 0.236 Total Nitrogen Geometric Mean (mg/L) 1.22

The Habitat A ssessment sc ore was in the “Optimal” range with 142 points. The SCI score (55) was in the

“ Healthy ” category, exhibiting a div erse assemblage of sensitive invertebrates and indicating the site is meeting its Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 98.33 % Chlorophycota 1.33 % Cryptophycophyta 0 % C yanophycota 0.33 Number of Taxa 48

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 48 algal taxa observed, the majority of the periphyton communit y was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Falling Cree k @ C -131. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations refle ct ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -17 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Little Creek @ US 441

STORET Station 21010033

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.18, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicul ture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Little Creek @ US 441 (Station 21010033) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 ae rial photograph of Little Creek @ US 441 (Station 21010033) showing close-up of sampling site.

Photograph of Little Creek @ US 441Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -18 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 127 Hydrologic Score 1 LDI Score 1.1 8 SCI Score 89 A Total Phosphorus Geometric Mean (mg/L) 0.373 Total Nitrogen Geometric Mean (mg/L) 0.72

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, indicating an exceedingly diverse assemb lage of sensitive macroinvertebrates, and demonstrating that the site is clearly meeting its designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 89.00 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 10.67 Number of Taxa 53

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 53 algal taxa observed, the majority of the periphyton commu nity was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and macroinvertebrate and algal communities) at Little Cr eek @ US 441. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations re flect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -19 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## New River @ SR 18 STORET Station 21030049

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.28, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicul ture and pasture located beyond a forested buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of New River @ SR 18 (Station 21030049) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of New River @ SR 18 (Station 21030049) showing close-up of sampling site.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -20 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 130 Hydrologic Score 2 LDI Score 1.28 SCI Score 76 Total Phosphorus Geometric Mean (mg/L) 0.229 Total Nitrogen Geometric Mean (mg/L) 1.04

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, indicating an unusually diverse commun ity of macroinvertebrates, and demonstrating that the water body is definitively meeting its designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 75.62 % Chlorophycota 0.50 % Cryptophycophyta 0 % Cyanophycota 23.88 Number of Taxa 72

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 72 algal taxa observed, the majority of the periphyton commu nity was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at New River @ SR 18. Based on ou r an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally dist urbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -21 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchma rk Site Summary 

## Olustee Creek @ SR 100

STORET Station UNI234LV

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.32, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and pasture locat ed well beyond a forested buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Olustee Creek @ SR 100 (Station UNI234LV) showing watershed vicinity and land use.

Image Remo ved Due to Copyright. DOQQ 2004 aerial photograph of Olustee Creek @ SR 100 (Station UNI234LV) showing close -up of sampling site.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -22 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 118 Hydrologic Score 2 LDI Score 1.32 SCI Score 44 A Total Phosphorus Geometric Mean (mg/L) 0.119 Total Nitrogen Geometric Mean (mg/L) 2.05

The Habitat A ssessment sc ore was in the high end of the “Suboptimal” range, predominantly due to low water velocity. The SCI score was in the “Healthy” category, indicating a diverse community of sensitive invertebrates, and demonstrating that the water body is meeting its designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 95.77 % Chlorophyco ta 2.82 % Cryptophycophyta 0 % Cyanophycota 1.41 Number of Taxa 57

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 57 algal taxa observed, the m ajority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and macroinvertebrate and algal communities) at Olustee Creek @ SR 100. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only ar e the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -23 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Sampson River @ SW 106 Ave STORET Station 3598-B

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.68, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and pasture beyond a forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Sampson River @ SW 106 Ave (Station 3598-B) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Sampson River @ SW 106 Ave (Station 3598-B) showing close-up of sampling site.

Photograph of Sampson River @ SW 106 AveTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -24 Habi tat, Biological and Water Quality Data

Parameter Value Habitat Assessment 131 Hydrologic Score 4 LDI Score 1.68 SCI Score 65 A Total Phosphorus Geometric Mean (mg/L) 0.342 Total Nitrogen Geometric Mean (mg/L) 0.68

The Habitat A ssessment score was in the “Optimal” range. The hydrologic score was somewhat elevated due to presence of a control structure at outlet of Lake Sampson, however, local residents indicated that this structure had not been operational for at least 6 months, meaning the contemporaneous biological community had recovered from any potential hydrologic modification. The SCI score was in the “ Healthy ” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 58.33 % Chlorophycota 1.33 % Cryptophycophyta 0 % Cyanophycota 40.33 Number of Taxa 63

Minimal periphyton was observed at the site, which would allow opti mal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 63 algal taxa observed, the majority of the periphyton community was composed of diatoms, with a large proportion of blue- green algae

.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Sampson River @ SW 106 Ave. Based on our analyses, the Department concluded that the site was influenced only by low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -25 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Santa Fe River @ Worthing ton Springs STORET Station SFR030C1

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.88, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and pasture beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Santa Fe River @ Worthington Springs (Station SFR030C1) showing watershed vicinity and land use.

Ima ge Removed Due to Copyright. DOQQ 2004 aerial photograph of Santa Fe River @ Worthington Springs (Station SFR030C1) showing close-up of sampling site.

Photograph of Santa Fe River @ Worthington SpringsTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -26 Habitat, Biological and Water Quality Data

P arameter Value Habitat Assessment 132 Hydrologic Score 2 LDI Score 1.88 SCI Score 64 A Total Phosphorus Geometric Mean (mg/L) 0.235 Total Nitrogen Geometric Mean (mg/L) 1.15

The Habitat A ssessment score was in the “Optimal” range. The SCI score wa s in the “ Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is meeting the Class III designated

use.
Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 61.33 % Chlorophycota 3.00 % Euglenophycota 0.33 % Cyanophycota 35.33 Number of Taxa 64

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 64 algal taxa ob served, the majority of the periphyton community was composed of diatoms, with a large proportion of blue- green algae

.
Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessm ent, hydrology, local land use, and algal and macroinvertebrate communities) at Santa Fe River @ Worthington Springs. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagat ion and maintenance of a healthy, well -balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -27 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Suwannee River @ CR 6 STORET Station 3535

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.22, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site vis it confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and pasture beyond an extensive forested riparian buffer zone. The vast majority of the basin lies within the Okeefenokee National Wildlife Refug e, an area of pristine water quality conditions. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Suwannee River @CR 6 (Station 3535) showing watershed vicinity and land

use

Im age Removed Due to Copyright. DOQQ 2004 aerial photograph of the Suwannee River @ CR 6 (Station 3535) showing close-up of sampling site

Photograph of the Suwannee River @ CR 6Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -28 Habitat, Biological and Water Quality Data

Parameter Value Habitat Asses sment 115 Hydrologic Score 1 LDI Score 1.22 SCI Score 52 A Total Phosphorus Geometric Mean (mg/L) 0.078 Total Nitrogen Geometric Mean (mg/L) 1.34

The Habitat A ssessment sc ore was in high end of the “Suboptimal” range. The SCI was not conducted due to a limited amount of habitat in very shallow water. Water levels had recently increased, and the habitat was not inundated for a sufficient period to comply with the Standard Operating Procedure. However, this site was sampled for SCI on 10/10/2006 and 12/12/2007 and received a 53 and 51, respectively, both in the “Healthy” SCI category.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 91.48 % Chlorophycota 6.89 % Cryptophycophyta 0 % Cyanophycota 1.64 Numb er of Taxa 45

Extensive periphyton growth was observed at this site , which is thought to be a natural phenomenon associated with the limerock substrate and very low water conditions. Of the 45 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at the Suwannee River at CR 6. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that the algal growth observed was a natural phenomenon. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -29 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Sit e Summary 

## Swift Creek @ CR 239

STORET Station 21030088

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.26, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with silvicultural activities beyond a forested buffer

zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Swift Creek @ CR 239 (Station 21030088) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Swift Creek @ CR 239 (Station 21030088) showing close-up of sampling site.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -30 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 105 Hydrologic Score 2 LDI Score 1.26 SCI Score NA Total Phosphoru s Geometric Mean (mg/L) 0.083 Total Nitrogen Geometric Mean (mg/L) 2.06

The Habitat A ssessment score was in the “Suboptimal” range. There was no water flow at the time of sampling, therefore the SCI was not conducted, according to the SCI Standard Operating Procedure.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 99.67 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 0 Number of Taxa 32

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 32 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientis ts conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at Swift Creek @ CR 239. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that no adverse algal or aquatic plant issues were present. T hus, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -31 NORTH CENTRAL NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Robinson Branch @ C

- 246 STORET Station ROB01C1

Land Use

This station was considere d to be a candidate reference site based on the Landscape Development Intensity Index score of 1.36, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and unimproved pasture beyond a forested riparian buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Robinson Branch @ C-246 (Station ROB01C1) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Robinson Branch @ C-246 (Stat ion ROB01C1) showing close-up of sampling site.

Photograph of Robinson Branch @ C-246Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -32 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 2 LDI Score 1.36 SCI Score 81 A Total Phosphorus Geometr ic Mean (mg/L) 0.158 Total Nitrogen Geometric Mean (mg/L) 0.95

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting an unusually diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 97.33 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 2.33 Number of Taxa 58

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 58 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Refer ence Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Robinson Branch @ C-246. Based on our analyses, the De partment concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -33 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Allig ator Creek @ US 301 & SR115 STORET Station 19020052

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.74, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and unimproved pasture gener ally beyond a 100 m forested riparian zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Alligator Creek @ US 301 & SR 115 (Station 19020052) showing watershed vicinity and land use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Alligator Creek @ US 301 & SR 115 (Station 19020052) showing close-up of sampling site.

Photograph of Alligator Creek @ US 301 & SR 115Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -34 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 137 Hydrologic Score 2 LDI Score 1.74 SCI Score 63 A Total Phosphorus Geometric Mean (mg/L) 0.190 Total Nitrogen Geometric Mean (mg/L) 1.12

The Habitat A ssessment score was in the “Optimal” range. The SCI score w as in the “ Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is meeting the Class III designated

use.
Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 83.02 % Chlorophycota 1.89 % Cryptophycophyta 0 % Cyanophycota 15.09 Number of Taxa 64

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 64 algal taxa ob served, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Alligator Creek @ US 301 & SR 115. Based on

our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assembl ages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -35 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Ates Creek @ CR 315 STORET Station CLA243LV

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity I ndex score of 1.18, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the wate rshed land use was forest/natural with some silviculture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Ates Creek @ CR 315 (Station CLA243LV) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Ates Creek @ CR 315 (Station CLA243LV) showing close-up of sampling site.

Photograph of Ates Creek @ CR 315Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -36 Habitat, Biological and Water Quality Data

Parameter Value Habitat As sessment 144 Hydrologic Score 2 LDI Score 1.18 SCI Score 91 A Total Phosphorus Geometric Mean (mg/L) 0.198 Total Nitrogen Geometric Mean (mg/L) 0.71

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” cat egory, exhibiting a n exceedingly diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 98.00 % C hlorophycota 1.00 % Cryptophycophyta 0 % Cyanophycota 0.33 Number of Taxa 41

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 41 algal taxa obser ved, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Ates Creek @ CR 315. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fis h and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -37 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Black Creek @ SR 16 STORET Station CLA254LR

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1. 21, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Black Creek @ SR 16 (Station CLA254LR) showing watershed vicinity and land

use.
Image Remo ved Due to Copyright. DOQQ 2004 aerial photograph of Black Creek @ SR 16 (Station CLA254LR) showing close-up of sampling site.

Photograph of Black Creek @ SR 16Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -38 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 133 H ydrologic Score 2 LDI Score 1.21 SCI Score 91 A Total Phosphorus Geometric Mean (mg/L) 0.116 Total Nitrogen Geometric Mean (mg/L) 0.55

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiti ng a n unusually diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting its Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 94.67 % Chlorophycota 1.33 % Cryptophycophyta 0 % Cyanophycota 4.00 Number of Taxa 54

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 54 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate c ommunities) at Black Creek @ SR 16. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutr ient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -39 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Greens Creek @ CR 315 STORET Station GC315

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.33, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natura l with some silviculture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of Greens Creek @ CR 315 (Station GC315) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Greens Creek @ CR 315 (Station GC315) showing close-up of sampling site.

Photograph of Greens Creek @ CR 315Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -40 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 114 Hydrologic Score 2 LDI Score 1.33 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.020 Total Nitrogen Geometric Mean (mg/L) 0.57

The Habitat A ssessment sc ore was in the high “Suboptimal” range, mostly because of the lack of water velocity. Stream Condition Index sampling was not performed at the time of sampling due to the stagnant water conditions. An adjacent station was sampled for SCI in 2005 and 2006, and both times scored in the “Healthy” category based on SCI_2007 thresholds. This suggests that the site is meeting its Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 98.05 % Chlorophycota 0.65 % Cryptophycophyta 0 % Cyanophycota 1.30 Number of Taxa 42

Very little periphyton was obs erved at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 42 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at Greens Creek @ CR 315. Based on our an alyses, the Department concluded that the site was influenced o nly by very low levels of anthropogenic stressors with no algae related issues. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with plants and algae which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -41 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Little St. Marys River@ CR 121 A STORET Station 19010046

Land Use

This station was c onsidered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.18, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed la nd use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with silvicultural activities beyond a forested riparian

zone . There are no point source discharges upstream of the site.

Image Remo ved Due to Copyright. DOQQ 2004 aerial photograph of Little St. Marys River @ CR 121 A (Station 19010046) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Little St. Marys River @ CR 121 A (Station 19010046) showing close- up of sampling site.

Photograph of Little St. Marys River @ CR 121 ATechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -42 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 117 Hydrologic Score 2 LDI Score 1.18 SCI Score NA Total Phosphorus Geomet ric Mean (mg/L) 0.048 Total Nitrogen Geometric Mean (mg/L) 1.15

The Habitat A ssessment sc ore was in the high end of the “Suboptimal” category, predominantly sue to low water velocity. Due to the swamp

- like nature of this system, SCI sampling was not ap propriate and was not conducted.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 61.64 % Chlorophycota 5.90 % Euglenophycota 1.97 % Cyanophycota 29.84 Number of Taxa 75

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 75 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:

Expe rienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at Little St. Marys River @ CR 121 A. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota which demo nstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -43 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Middle Prong St. Marys River @ CR 125 STORET Station 19010041

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.16, suggesting benign human influences in the watershed. Before and during the site visit, aerial photogr aphs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and pasture located beyond a 100 m forested buffer. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Middle Prong St. Marys @ CR 125 (Station 19010041) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Middle Prong St. Marys @ CR 125 (Station 19010041) showing close- up of sampling site.

Photograph of Middle Prong St. Marys @ CR 125Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -44 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 137 Hydrologic Score 2 LD I Score 1.16 SCI Score 78 A Total Phosphorus Geometric Mean (mg/L) 0.029 Total Nitrogen Geometric Mean (mg/L) 1.10

The Habitat A ssessment score was in the “Optimal” range. Despite the naturally low dissolved oxygen measured during sampling (2.7 mg/L) the SCI score was in the “Exceptional” category, exhibiting an unusually diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting its Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 81.40 % Chlorophycota 1.33 % Cryptophycophyta 0 % Cyanophycota 17.28 Number of Taxa 55

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal gr owth was found. Of the 55 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, h ydrology, local land use, and algal and macroinvertebrate communities) at Middle Prong St. Marys @ CR 125. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fish and

w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -45 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Middle Prong St. Marys River @ CR 127 STORET Station MPS

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.34, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of Middle Prong St. Marys @ CR 127 (Station MPS) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Middle Prong St. Marys @ CR 127 (Station MPS) showing close-up of sampling site.

Photograph of Middle Prong St. Marys @ CR 127Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -46 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 131 Hydrologic Score 2 LDI Score 1.34 SCI Score 71 A Total Phosphorus Geometric Mean (mg/L) 1.09 Total Nitrogen Geometric Mean (mg/L) 0.026

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting an unusually diverse assemblage of sensitive invertebrates and indicating the site is certainly meeting its Class III designated use

. Periphyton Community Composi tion

Algal Community Structure % Composition % Bacillariophyta 97.33 % Chlorophycota 0.67 % Cryptophycophyta 0 % Cyanophycota 2.00 Number of Taxa 42

Very little periphyton was observed at the site, which would allow optimal substrate use by macroin vertebrates. No nuisance algal growth was found. Of the 42 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and me asurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Middle Prong St. Marys @ CR 127. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogen ic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive de signated 

### use ( propagation and maintenance of a healthy, well -balanced population of fish and

w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -47 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## North Fork Black Creek @ Jennings Landing STORET Station 14264

Land Use

This station was c onsidered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.62, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed la nd use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and pasture located beyond an extensive forested riparian buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of North Fork Black Creek @ Jennings Landing (Station 14264) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of North For k Black Creek @ Jennings Landing (Station 14264) showing close-up of sampling site.

Photograph of North Fork Black Creek @ Jennings LandingTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -48 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 137 Hydrologic Score 2 LDI Score 1.62 SCI Score 73 A Total Phosphorus Geometric Mean (mg/L) 0.038 Total Nitrogen Geometric Mean (mg/L) 0.51

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting an unusually divers e assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 69.33 % Chlorophycota 1.33 % Cryptophycoph yta 0 % Cyanophycota 29.33 Number of Taxa 48

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 48 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at N orth Fork Black Creek @ Jennings Landing. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are th e nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well

balanced population of fi sh and w ildl ife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -49 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary



## Peters Creek @ CR 315 A STORET Station CLA246GS

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.21, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and pasture located beyond a forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Peters Creek @ CR315A (Station CLA246GS) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Peters Creek @ CR315A (Station CLA246GS) showing close-up of sampling site.

Photograph of Peters Creek @ CR315ATechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -50 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 148 Hydrologic Score 2 LDI Score 1.21 SCI Score 73 A Total Phosphorus Geometric Mean (mg/L) 0.019 Total Nitrogen Geometric Mean (mg/L) 0.40

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composi tion % Bacillariophyta 94.00 % Chlorophycota 2.67 % Euglenophycota 0.67 % Cyanophycota 2.67 Number of Taxa 64

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth wa s found. Of the 64 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrolog y, local land use, and algal and macroinvertebrate communities) at Peters Creek @ CR 315 A. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained heal thy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a h ealthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -51 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## South Fork Black Creek @ SR 21 STORET Station 20030481

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.35, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confir med that the majority of the watershed land use was forest/natural with some silvicultural activities beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 a erial photograph of South Fork Black Creek @ SR 21 (Station 20030481) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of South Fork Black Creek @ SR 21 (Station 20030481) showing close -up of sampling si te.

Photograph of South Fork Black Creek @ SR 21Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -52 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 144 Hydrologic Score 1 LDI Score 1.35 SCI Score 83 A Total Phosphorus Geometric Mean (mg/L) 0.093 Total Nitrogen G eometric Mean (mg/L) 0.50

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting a n exceedingly diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting i ts Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 72.61 % Chlorophycota 0.99 % Cryptophycophyta 0 % Cyanophycota 26.40 Number of Taxa 67

Very little periphyton was observed at t he site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 67 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experience d FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at South Fork Black Creek @ SR 21. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with bio ta which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and wildlife)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -53 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## St. Marys River @ SR 2

STORET Stat ion 19010006

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.09, suggesting benign human influences in the watershed. Before and during the site visit, aerial photograph s were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and unimproved pasture beyond an extensive forested riparian buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of St. Marys at SR 2 (Station 19010006) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of St. Marys at SR 2 (Station 19010006) showing close -up of sampling site.

Photograph of St. Marys at SR 2Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -54 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 124 Hydrologic Score 1 LDI Score 1 .09 SCI Score 100 A Total Phosphorus Geometric Mean (mg/L) 0.017 Total Nitrogen Geometric Mean (mg/L) 1.27

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, indicating an exceedingly diverse com munity of sensitive invertebrates, and demonstrating that the site is definitively meeting its Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 93.33 % Chlorophycota 4.00 % Eugleno phy co ta 0.33 % Cyanophycota 2.33 Number of Taxa 54

No nuisance algal growth was found. Of the 54 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at St. Marys River at SR 2. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -55 NORTHEAST NUTRIENT REGION

Nutrient Benchmark Site Summary 

## St. Marys River @ Tompkins Landing STORET Station 19010077

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.22, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and pasture beyond an extensive forested riparian buffer zone. Ther e are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of St. Marys at Tompkins Landing (Station 19010077) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aeri al photograph of St. Marys at Tompkins Landing (Station 19010077) showing close-up of sampling site.

Photograph of St. Marys at Tompkins LandingTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -56 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 114 Hydrologic Score 2 LDI Score 1.22 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.043 Total Nitrogen Geometric Mean (mg/L) 0.87

The Habitat A ssessment sc ore was in the high end of the “Suboptimal” range (mostly due to limited substrate availability associated wi th low water conditions). Since very little habitat was submerged due to the unrepresentative low water level, SCI sampling was determined to be inappropriate (according to the Standard Operating Procedure) and was not conducted.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 75.50 % Chlorophycota 8.94 % Cryptophycophyta 0 % Cyanophycota 15.56 Number of Taxa 73

No visual evidence of algal growth or adverse aquatic growth was observed at this site. Of the 73 algal taxa found, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land u se, and algal communities) at St. Marys River at Tompkins Landing. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that no adverse algal or aquatic plant issues were presen t. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population o f f i sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -57 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Aucilla River @ Highway 90 STORET Station 22040004

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Ind ex score of 1.06, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some field/pasture located well beyond the extensive riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Aucilla River (St ation 22040004) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Aucilla River (Station 22040004) showing close-up of sampling site.

Photograph of the Aucilla River at Highway 90Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -58 Habitat, B iological and Water Quality Data

Parameter Value Habitat Assessment 126 Hydrologic Score 2 LDI Score 1.06 SCI Score 61 A Total Phosphorus Geometric Mean (mg/L) 0.090 Total Nitrogen Geometric Mean (mg/L) 3.10

The Habitat A ssessment sc ore was in the “Optimal” range. The SCI score was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 96.67 % Chlorophycota 1.33 % Cryptophycophyta 0 % Cyanophycota 2.00 Number of Taxa 30

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 30 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Aucilla River at Highway 90. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearl y maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -59 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Blackwater River @ Highway 4 NW of Baker STORET Station 3545

Land Use
This station was considered to be a candidate reference site based on the Landscape Develop ment Intensity Index score of 1.04, suggesting benign human influences in the watershed. Before and during t he site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majo rity of the watershed land use was forest/natural with some silviculture . There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of the Blackwater River (Station 21FLGW_3545) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Blackwater River (Station 21FLGW_3545) showing close-up of sampling site.

Photograph of the Blackwater River (Station 21FLGW_3545)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -60 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 1 LDI Score 1.04 SCI Score 70 A Total Phosphorus Geometric Mean (mg/L) 0.009 Total Nitrogen Geometric Mean (mg/L) 0.40

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % C omposition % Bacillariophyta 88.45 % Chlorophycota 2.31 % Cryptophycophyta 0 % Cyanophycota 2.31 % Euglenophycota 0.33 Number of Taxa 39

Very little periphyton was observed at this site, and there was no nuisance algal growth. Of the 39 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal a nd macroinvertebrate communities) at the Blackwater River at Highway 4 NW of Baker. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biol ogical assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organi sms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -61 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Econfina Creek @ Scott Road STORET Station 32030023

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.57, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natur al with silvicultural activities beyond the extensive riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Econfina Creek (Station 32030023) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Econfina Creek (Station 32030023) showing close-up of sampling site.

Photograph of Econfina Creek at Scott Rd.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -62 Habitat, Biological and Water Quality Data

Parameter Va lue Habitat Assessment 130 Hydrologic Score 1 LDI Score 1.57 SCI Score 81 A Total Phosphorus Geometric Mean (mg/L) 0.010 Total Nitrogen Geometric Mean (mg/L) 0.26

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional ” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 89. 70 % Chlorophycota 5.65 % Cryptophycophyta 0 % Cyanophycota 3.65 % Euglenophycota 1.00 Number of Taxa 48

Minimal periphyton growth was observed at this site, which would allow for optimal substrate use by macroinvertebrates

. There was no nuisance al gal growth. Of the 48 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydro logy, local land use, and algal and macroinvertebrate communities) at Econfina Creek at Scott Road. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintai ned healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -63 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Econfina River @ Highway 98 STORET Station TAY170LR

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Inde x score of 1.14, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use . Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture beyond an extensive riparian buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Econfina River (Station TAY170LR) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Econfina River (Station TAY170LR) showing close-up of sampling site.

Photograph of the Econfina River at Hwy 98Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -64 Habitat, Biological and Wat er Quality Data

Parameter Value Habitat Assessment 99 Hydrologic Score 1 LDI Score 1.14 SCI Score 41A Total Phosphorus Geometric Mean (mg/L) 0.066 Total Nitrogen Geometric Mean (mg/L) 1.21

The Habitat A ssessment sc ore was in the “Suboptimal” range due mostly to low water velocity (<0.05 m/s ) and limited availability of wetted habitat, making natural drought the most influential stressor during the most recent site visit. Prior to this event, the two most recent SCI samples for this site , which were collected during normal water levels on 2/23/06 and 8/2/05, scored 40 and 42 , respectively. B oth of these score were in the “Healthy” category, and the average of these scores is shown in the table . From 1996 to 1998, a total of 18 SCIs were performed a t a site located approximately 3000 m above Highway 98 as part of a study to evaluate the effectiveness of forestry best management practices. All 18 of these SCIs scored in the category referred to as “excellent” in that version of the index, indicating that this site has a long demonstrated history of healthy biota.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 86.67 % Chlorophycota 7.0 % Cryptophycophyta 0 % Cyanophycota 6.33 Number of Taxa 36

Filame ntous algal growth was observed on the shallow, rocky substrate in the first 20 m of the site, but very little periphyton was observed in the rest of the stretch. This patch of filamentous algae was likely favored by the low water conditions. Of the 36 al gal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Econfina River at Hwy 98. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological a ssemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -65 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Escambia River @ Highway 4 STORET Station 3549

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.27, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and field/pasture beyond an extensive riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Escambia River (Station 21FLGW_3549) showin g watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Escambia River (Station 21FLGW_3549) showing close-up of sampling site. An extensive forested buffer is present on both sides of the river.

Photograph of the Escambia River (Station 21FLGW_3549)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -66 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 120 Hydrologic Score 2 LDI Score 1.27 SCI Score 51 A Total Phosphorus Geometric Mean (mg/L) 0.041 Total Nitrogen Geometri c Mean (mg/L) 0.55

The Habitat A ssessment score was in the “Optimal” range, although substrate availability was somewhat limited due to the overall large size of the river. The SCI score was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 84.72 % Chlorophycota 5.98 % Cryptophycophyta 0 % Cyanophycota 9.30 Number of Taxa 43

Very little periphyton was observed at this site, and there was no nuisance algal growth. Of the 4 3 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Escambia River at Highway 4. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -67 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Escambia River @ Highway 184 STORET Station 33020007 / 3541

Lan d Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.18, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined t o assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and agricultural activities well beyond an extensive riparian buffer zone . There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Escambia River @ 184 showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Escambia River @ 184 showing close- up of sampling site. An extensive forested buffer is present on both sides of the river.

Photograph of the Escambia River at Highway 184 (Station 21FLGW_3541)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -68 Habitat, Biological and Water Quality Data

Parameter Value Ha bitat Assessment 115 Hydrologic Score 2 LDI Score 1.18 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.035 Total Nitrogen Geometric Mean (mg/L) 0.53

The Habitat A ssessment sc ore was in the high end of the “Suboptimal” range, predominantly due to lack of water velocity and limited substrate availability, related to the overall large size of the system. SCI sampling was not conducted because of the “lake

- like” conditions present, and lack of water velocity in areas where accessible habitats were located, in accordance with the Standard Operating Procedures.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 83.72 % Chlorophycota 1.66 % Cryptophycophyta 0 % Cyanophycota 14.62 Number of Taxa 52

Mini mal periphyton growth was observed at this site, which would allow for optimal substrate use by benthic invertebrate and fish communities

. Of the 52 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, algal community, and local land use) at the Escambia River at Highway 184. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that no adverse algal or aquatic plant issues were present. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -69 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Mule Creek @ SR 12 STORET Stati on LIB104LV

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.31, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the watershed land use was predominately forest/natural with some silviculture. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Mule Creek (Station LIB104LV) showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Mule Creek (Station LIB104LV) showing close-up of sampling site

Photograp h of Mule CreekTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -70 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 132 Hydrologic Score 2 LDI Score 1.31 SCI Score 67 A Total Phosphorus Geometric Mean (mg/L) 0.033 Total Nitrogen Geometric Mean (mg/L) 0.50

The Habit at A ssessment score was in the “Optimal” range. The SCI scored in the “Exceptional” category, therefore Mule Creek hosts a diverse community of sensitive aquatic macroinvertebrates.

Periphyton Community Composition

Algal Community Structure % Compositi on % Bacillariophyta 99.0 % Chlorophycota 0 % Cryptophycophyta 0 % Cyanophycota 1.0 Number of Taxa 22

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of t he 22 algal taxa observed , the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measu rements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Mule Creek. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balance d populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -71 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Quincy Creek above 267

STORET Station 22020093

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Inte nsity Index score of 1.34, suggesting benign human influences in the watershed. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with silviculture , agriculture, and field/pasture located beyond an adequate riparian buffer zone . There are no direct point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Quincy Creek (Station 22020093) showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Quincy Creek near 267 (Station 22020093) showing close-up of sampling site

Photograph of Quincy Creek upstream of 267Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -72 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 1 22 Hydrologic Score 3 LDI Score 1.34 SCI Score 57 A Total Phosphorus Geometric Mean (mg/L) 0.110 Total Nitrogen Geometric Mean (mg/L) 0.58

The Habitat A ssessment score was in the “Optimal” range. Extensive deposits of “pipe clay” (Attapulgite) were noted throughout the stream, a natural geologic feature which would be expected to contribute total phosphorus to the water. T he Stream Condition Index scored in the “H ealthy” category, indicating a diverse assemblage of macroinvertebrates, and demonstrati ng that the site is meeting its Class III designated use.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 93.0 % Chlorophycota 2.0 % Cryptophycophyta 0 % Cyanophycota 5.0 Number of Taxa 53

Minimal perip hyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 53 algal taxa observed , the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Quincy Creek, upst ream of 267. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations ref lect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -73 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Sopch oppy River STORET Station WAK158LR

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.13, suggesting benign human influences in the watershed. Before the site visit, aeria l photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural, with some limited silviculture beyond an extensive riparian buffer zone. The w atershed lies within the Apalachicola National Forest. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Sopchoppy River (Station WAK158LR) showing watershed vicinity and land

u se.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Sopchoppy River (Station WAK158LR) showing close-up of sampling site. An extensive riparian forested buffer is present on both sides of the system.

Photograph of the Sopchoppy Riv er (Station WAK158LR)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -74 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 1 LDI Score 1.13 SCI Score 64 A Total Phosphorus Geometric Mean (mg/L) 0.026 Total Nitrogen Geometric Mean (mg/L) 0.60

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Healthy” category, indicating that the site contains a diverse assemblage of sensitive invertebrates and is meeting its Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 73.05 % Chlorophycota 4.55 % Cryptophycophyta 0 % Cyanophycota 22.40 Number of Taxa 54

While a small amount of filamentous algae was found, the majority of the site had minimal periphyton growth. Of the 54 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrolo gy, local land use, and algal and macroinvertebrate communities) at the Sopchoppy River. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic o rganisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -75 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## St. Marks River STORET Station WAK168LR

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.40, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the watershed land use was predominately forest/natural wit h some silviculture beyond a forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Saint Marks River (Station WAK168LR) showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Saint Marks River (Station WAK168LR) showing close-up of sampling site

Photograph of the Saint Marks RiverTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -76 Habitat, Biological and Water Quality Data

Parameter Value Ha bitat Assessment 155 Hydrologic Score 2 LDI Score 1.40 SCI Score 45 A Total Phosphorus Geometric Mean (mg/L) 0.040 Total Nitrogen Geometric Mean (mg/L) 0.25

The Habitat A ssessment score was in the “Optimal” range. The SCI scored in the “Healthy” ca tegory, which indicates a diverse assemblage of aquatic invertebrates, and demonstrated that the stream is meeting its Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 91.36 % Chlorop hycota 0.66 % Cryptophycophyta 1.00 % Cyanophycota 6.98 Number of Taxa 43

Nuisance algal growth was not an issue at this site, although moderate periphyton growth was observed in isolated areas of the sampling reach, thought to be a natural condition. There was no habitat smothering (this element scored in the optimal range). Of the 43 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a s eries of observations and measurements (habitat assessment, hydrology, local land use, and algal and aquatic invertebrate communities) at the Saint Marks River. Based on our an alyses, the Department concluded that the site was influenced only by very low l evels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -77 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Swamp Creek at SR 159 STORET Station S232

Land Use

This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.51, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use w as forest/natural, with minimal areas of silviculture and field/pasture located beyond an extensive forested riparian buffer zone

. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Swamp Creek @ SR 159 (Station S232) showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Swamp Creek @ SR 159 (Station S232) showing close-up of sampling site

Photograph of Swamp Creek at SR 159Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -78 Habi tat, Biological and Water Quality Data

Parameter Value Habitat Assessment 112 Hydrologic Score 2 LDI Score 1.51 SCI Score 49 A Total Phosphorus Geometric Mean (mg/L) 0.170 Total Nitrogen Geometric Mean (mg/L) 0.75

The Habitat A ssessment score was in the high end of the “Suboptimal” range. The Stream Condition Index scored in the “ H ealthy” category, indicating a diverse assemblage of macroinvertebrates, and demonstrating that the site is meeting its Class III designated use.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 67.43 % Chlorophycota 5.92 % Cryptophycophyta 0 % Cyanophycota 25.99 % Euglenophycota 0.66 Number of Taxa 65

Minimal periphyton growth was observed at the site, which would allo w for optimal substrate use by benthic invertebrate and fish communities. Of the 6 5 algal taxa observed , the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusi on:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Swamp Creek at SR 159. Based on our an alyses, the Department concluded t

hat the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -79 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary



## Telogia Creek @ CR 1641

STORET Station NUTREF001

Land Use

This st ation was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.24, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed la nd use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Telogia Creek (Station NUTREF001) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Telogia Creek (Station NUTREF001) showing close-up o f sampling site.

Photograph of Telogia Creek at CR 1641Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -80 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 131 Hydrologic Score 1 LDI Score 1.24 SCI Score 78 A Total Phosphorus Geometric Mean (mg/L) 0.067 Total Nit rogen Geometric Mean (mg/L) 0.47

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive aquatic invertebrates, definitively supporting the Class I II designation.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 85.0 % Chlorophycota 0.33 % Cryptophycophyta 0 % Cyanophycota 14.67 Number of Taxa 40

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 40 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scienti sts conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Telogia Creek at CR 1641. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -81 PANHANDLE NUTRIENT REGION

Nutrient Reference Site Summary 

## Wacissa River near Big Blue Spring STORET Station 22040009

Land Use

This station was conside red to be a candidate reference site based on the Landscape Development Intensity Index score of 1.03, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct ob servations during the site visit confirmed that the majority of the watershed land use was forest/natural with silvicultural activities beyond and extensive forested riparian buffer

zone. There are no point source discharges upstream of the site.

Image Re moved Due to Copyright. DOQQ 2004 aerial photograph of the Wacissa River (Station 22040009) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Wacissa River (Station 22040009) showing close -up of sampling site.

Photograph of the Wacissa River near the headwaters.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -82 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 138 Hydrologic Score 1 LDI Score 1.03 SCI Score 40 A Total Phosphorus Geometric Mean (mg/L) 0.046 Total Nitrogen Geometric Mean (mg/L) 0.29

The Habitat A ssessment score was in the high end of the “Suboptimal” range. The SCI score was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use

. Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 85.43 % Chlorophycota 2.65 % Cryptophycophyta 0 % Cyanophycota 11.92 Number of Taxa 50

There was a minimal amount of periphyton at this site, with no nuisance algal growth. Of the 50 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observati ons and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Wacissa River near Big Blue Spring. Based on

our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -83 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Yellow River at Highway 2 STORET Station 3546

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.24, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit co nfirmed that the majority of the watershed land use was forest/natural with some silviculture beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Yellow River at Hwy 2 (Station 21FLGW_ 3546) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Yellow River (Station 21FLGW_ 3546) showing close-up of sampling site. An extensive riparian forested buffer is present on both sides of the system.

Photograph of the Yellow River near Hwy 2Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -84 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 137 Hydrologic Score 1 LDI Score 1.24 SCI Score 79 A Total Phosphorus Geometric Mean (mg/L) 0.024 Total Nitrogen Geometric Mean (mg/L) 0.40

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is definitively meeting the Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 90.07 % Chlorophycota 1.99 % Cryptophycophyta 0 % Cyanophycota 7.95 Number of Taxa 50

Very little periphyton was observed at this site, and there was no nuisance algal growth. Of the 50 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Yellow River at Highway 2. Based on our analyses, the Department concluded that the site was influenc ed only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated t o be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -85 PANHANDLE NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Yon Creek @ SR 12 STORET Station GAD106GS

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.26, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations duri ng the site visit confirmed that the majority of the watershed land use was forest/natural with some silviculture and field/pasture located beyond an extensive forested riparian buffer zone. There are no known point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Yon Creek (Station GAD106GS) showing watershed vicinity and land use.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Yon Creek (Station GAD106GS) showing close-up of sampling site.

Photograph of Yon Creek @ SR 12Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -86 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 137 Hydrologic Score 2 LDI Score 1.26 SCI Score 81 A Total Phosphorus Geometric Mean (mg/L) 0.083 Total Nitrogen Geometric Mea n (mg/L) 0.86

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Exceptional” category, indicating an unusually diverse community of sensitive aquatic invertebrates, and demonstrating that the stream is definitively meeting its Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 98.00 % Chlorophycota 0 % Cryptophycophyta 0 % Cyanophycota 2.00 Number of Taxa 23

Minimal periphyton growth was observed at this site, which would allow for optimal substrate use by macroinvertebrates . There was no nuisance algal growth. Of the 23 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Yon Creek @ SR 12. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -87 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Bee Branch STORET Station 28020299FTM

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.64, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majo rity of the watershed land use was forest/natural, with small areas of citrus and field/pasture observed beyond the forested buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of Bee Branch (Station 28020299FTM) showing watershed vicinity and land use. The headwaters drain an area dominated by pine flatwoods, prairie, and wetlands.

Image Removed Due to Copyright. DOQQ 2004 aerial l photograph of Bee Branch (Station 28020299FTM) showing close-up of sampling site. An extensive forested riparian zone was evident of both sides of the system.

Photographs of Bee Branch (Station 28020299FTM).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -88 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 147 H ydrologic Score 3 LDI Score 1.64 SCI Score 49 A Total Phosphorus Geometric Mean (mg/L) 0.080 Total Nitrogen Geometric Mean (mg/L) 1.13

The habitat assessment sc ored in the “Optimal” range. The Stream Condition Index scored in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use

. Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 91.33 % Chlorophycota 2.67 % Euglen ophy co ta 0.67 % Cyanophycota 4.67 Number of Taxa 60

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 60 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal community) at Bee Branch. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only a re the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -89 PENINSULA NUTRIENT REGION

Nutrient Reference Site Summary 

## Blackwater Creek at State Road 44A

STORET Station ALT_BWC44 (20010455)

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intens ity Index score of 1.09, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of sil viculture, and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Blackwater Creek at State Road 44A (Station BWC44 or 20010455) showing

watershed vicinity and land use. The system originates in Lake Norris, an undeveloped lake surrounded by wetland.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Blackwater Creek at State Road 44A (Station BWC44 or 20010455) showing close

- up of sampling site. An extensive forested riparian zone was evident on both sides of the system.

Photographs of Blackwater Creek at State Road 44ATechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -90 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 125 Hydrologic Score 2 LDI Score 1.09 SCI Score 77 A Total Phosphorus Geometric Mean (mg/L) 0.050 Total Nitrogen Geometric Mean (mg/L) 1.60

The habitat assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III designation.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 93.0 % Chloro phycota 0 % Cryptophycophyta 0 % Cyanophycota 7.0 Number of Taxa 45

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 45 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (hydrology, habitat assessme nt, local land use, algal and macroinvertebrate communities) at Blackwater Creek at State Road 44A. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintai ned healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fish and

w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -91 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Blackwater Creek upstream of Carter Prop Bridge STORET Station BWCCPB / 20010536

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.31, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 ae rial photograph of Blackwater Creek upstream of Carter Prop Bridge (Station BWCCPB) showing watershed vicinity and land use. The system originates in Lake Norris, an undeveloped lake surrounding by wetland.

Image Removed Due to Copyright. DOQQ aerial pho tograph of Blackwater Creek upstream of Carter Prop Bridge (Station BWCCPB) showing closeup of sampling site. An extensive forested riparian zone was evident of both sides of the system.

Photographs of Blackwater Creek upstream of Carter Prop BridgeTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -92 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 130 Hydrologic Score 2 LDI Score 1.31 Total Phosphorus Geometric Mean (mg/L) 0.054 Total Nitrogen Geometric Mean (mg/L) 1.28

The habitat assessment sc ored in the “Optimal” range. High water caused by recent rains made Stream Condition Index sampling inappropriate according to DEP standard operating procedures. Results from a Biorecon that was performed 2/7/2007 demonstrated that this site scored in the “healthy” r ange , indicating that the Class III designation is being fully supported.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 72.43 % Chlorophycota 15.28 % Cryptophycophyta 0.33 % Cyanophycota 7.31 % Euglenop hycota 2.99 Number of Taxa 72

Very little periphyton was observed at the site, which would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 72 algal taxa observed, the majority of the periphyton community was composed of diatoms.

.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (hydrology, local land use, and algal communities) at Blackwater Creek, upstream of Carter Prop Bridge. Base d on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimal ly disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated



### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -93 PENINSULA NUTRIENT REGION

Nutrient Ben chmark Site Summary 

## Cow Creek @ CR 138 STORET Station 21030086

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.61, suggesting benign human influences in the watershed. Before and during the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural with some silvicultural activities and field/pasture beyond an extensive forested riparian b uffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Cow Creek @ CR 138 showing watershed vicinity and land use

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Cow Creek @ CR 138 showing close-up of sampling site

Photograph of Cow Creek near CR 138Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -94 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 134 Hydrologic Score 2 LDI Score 1.61 SCI Score NA Total Phosphorus Geom etric Mean (mg/L) 0.114 Total Nitrogen Geometric Mean (mg/L) 0.39

The Habitat A ssessment sc ore was in the “Optimal” range. Stream Condition Index sampling was not performed at this site due to the low water level and the possibility of recent desiccatio n, in accordance with the Standard Operating Procedure.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 72.61 % Chlorophycota 1.32 % Cryptophycophyta 0 % Cyanophycota 25.74 Number of Taxa 64

Minimal perip hyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 64 algal taxa observed , the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at Cow Creek @ CR 138. Based on our an alys es, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that no adverse algal or aquatic plant issues were present. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed

c onditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -95 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary



## Cypr ess Branch above 78 STORET Station 28020239 / GLA630GS

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.21 suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with areas of citrus and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Cypress Branch (Station GLA630GS) showing watershed vicinity and land use. The stream originates in an area of prairie, flatwoods, and swamp.

I mage Removed Due to Copyright. DOQQ 2004 aerial photograph of Cypress Branch (Station GLA630GS) showing close-up of sampling site. An extensive riparian forested zone is generally present on both sides of the system throughout the watershed, with a minimu m of a 75-100 m buffer.

Photograph of Cypress Branch (Station GLA630GS).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -96 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 134 Hydrologic Score 2 LDI Score 1.21 SCI Score 63 A Total Phosphorus Geometric Mean (mg/L) 0 .131 Total Nitrogen Geometric Mean (mg/L) 1.97

The habitat assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Healthy” category, indicating a diverse assemblage of sensitive invertebrates, and fully supporting the Class III designation.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 91.33 % Chlorophycota 0 % Cryptophycophyta 0 % Cyanophycota 8.67 Number of Taxa 48

Very little periphyton was observed at the site, whi ch would allow optimal substrate use by macroinvertebrates. No nuisance algal growth was found. Of the 48 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusion:
Experienced FDEP scien tists conducted a series of observations and measurements (habitat assessment, hydrology, and local land use) at Cypress Branch. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressor s and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### us e (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -97 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Econlockhatchee River at Snowhill Road STORET Station ECH

Land Use

This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.14, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majorit y of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond the forested buffer zone.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Econlockhatchee River at Snowhill Road (Station ECH) showing watershed vicinity and land use. The system originates in the Econlockhatchee River Swamp, an extensive wetland area.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Econlockhatchee River at Snowhill Road (Station ECH) showin g close -up of sampling site. An extensive forested riparian zone was evident of both sides of the system.

Photographs of Econlockhatchee River at Snowhill RoadTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -98 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 1 06 Hydrologic Score 2 LDI Score 1.14 SCI Score 52 A Total Phosphorus Geometric Mean (mg/L) 0.098 Total Nitrogen Geometric Mean (mg/L) 0.93

The habitat assessment score of 106 placed this site in the high “Suboptimal” range. The Stream Condition Index score of 52 was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is clearly meeting the Class III designated use.

Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 90.31 % Chlorophycota 1.38 % Cryptophycophyta 0 % Cyanophycota 8.3 Number of Taxa 53

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 53 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measure ments (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Econlockhatchee River at Snowhill Road. Based

on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropog enic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -99 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Little Orange Creek STORET Station PUT308GS

Land Use

This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 2.01, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 200 4 aerial photograph of Little Orange Creek (Station PUT308GS) showing watershed vicinity and land

use. The stream originates from Little Orange Lake, an undeveloped system surrounded by forest and swamp.

Image Removed Due to Copyright. DOQQ 2004 aerial p hotograph of Little Orange Creek (Station PUT308GS) showing close-up of sampling site. An

extensive riparian forested buffer is generally present on both sides of the system throughout the watershed, with one small clear -cut area approaching within 75 m o f the stream.

Photograph of Little Orange CreekTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -100 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 131 Hydrologic Score 2 LDI Score 2.01 SCI Score 75 A Total Phosphorus Geometric Mean (mg/L) 0.201 Total Nitrogen Ge ometric Mean (mg/L) 1.13

The habitat assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class I II designated use. This is one of the few peninsular sites where Plecopterans (stoneflies, known to be extremely sensitive to stress) are routinely found.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 86.6 % Chlorophycota 5.7 % Cryptophycophyta 0 % Cyanophycota 7.4 Number of Taxa 48

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 48 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessmen t, hydrology, local land use, and algal and macroinvertebrate communities) at Little Orange Creek. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintain ed healthy biological assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well-balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -101 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Little Orange Creek below Cabbage Creek STORET Station LOCBCC

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.54, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Little Orange Creek below Cabbage Creek (Station LOCBCC) showing

watershed vicinity and land use. Little Orange Creek originates from Little Orange Lake, a completely undeveloped system surrounded by forest, and associated wetlands.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Little Orange Creek below Cabbage Creek (Station LOCBCC) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photographs of Little Orange Creek below Cabbage CreekTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -102 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 132 Hydrologic Score 2 LDI Score 1.54 SCI Score 89 A Total Phosphorus Geometric Mean (mg/L) 0.067 Total Nitrogen Geometric Mean (mg/L) 0.7 0

The habitat assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III designation.

Periph yton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 84.52 % Chlorophycota 5.16 % Cryptophycophyta 0 % Cyanophycota 10.32 Number of Taxa 54

Minimal periphyton growth was observed at the site, which would allow for op timal substrate use by benthic invertebrate and fish communities. Of the 54 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Little Orange Creek below Cabbage Creek. Based

on our an alyses, the Department c oncluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -103 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Moses Creek at US 1 STORET Station 27010050

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.24, suggesting benign human influences in the watershed. During the site visit, aerial photo graphs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond the forested buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright.

DOQQ 2004 aerial photograph of Moses Creek at US 1 (Station 27010050) showing watershed vicinity and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Moses Creek at US 1 (Station 27010050) showing close-up of sampling site. Moses Creek originates in an area of pine flatwoods and swamp. An extensive riparian forested buffer is present on both sides of the system.

Photograph of Moses Creek at US 1Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -104 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 138 Hydrologic Score 1 LDI Score 1.24 SCI Score 60 A Total Phosphorus Geometric Mean (mg/L) 0.081 Total Nitrogen Geometric Mean (mg/L) 1.03

The habitat assessment sc ored in the “Optimal” range. The Stream Condition Index score of 60 was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is meeting the Class III designated use.

Periphyton Community Composition

Algal Community Stricture % Co mposition % Bacillariophyta 14.33 % Chlorophycota 1.33 % Euglenophycota 1.67 % Cyanophycota 81.67 Number of Taxa 71

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish comm unities. Of the 71algal taxa observed, including 66 diatom taxa, the majority of the periphyton community was composed of blue- green algae. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scienti sts conducted a series of observations and measurements (habitat assessment, hydrology, and local land use) at Moses Creek at US 1. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stres sors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -105 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Orange Creek Upstream of Highway 21 STORET Station 21202

Land Use

This station was considered to b e a candidate reference site based on the Landscape Development Intensity Index score of 1.7, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture and field/pasture observed beyond a forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aeria l photograph of Orange Creek upstream of Highway 21 (Station 21202) showing watershed vicinity and land use. The stream originates from Orange Lake, an Outstanding Florida Water.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Orange Creek upstream of HWY 21 (Station 21202) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photograph of Orange Creek upstream of Highway 21Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -106 Habitat, Biological and Water Quality Da ta

Parameter Value Habitat Assessment 121 Hydrologic Score 3 LDI Score 1.74 SCI Score 74 A Total Phosphorus Geometric Mean (mg/L) 0.125 Total Nitrogen Geometric Mean (mg/L) 1.43

The habitat assessment scored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates. This is one of the few peninsular sites where Plecopterans (stoneflies, known to be extremely sensitive to stress) are routinely f ound.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 90.38 % Chlorophycota 1.37 % Cryptophycophyta 0 % Cyanophycota 8.25 Number of Taxa 51

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 51 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conc lusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Orange Creek, upstream of Highway 21. Based on

our an alyses, the Dep artment concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, t hey are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fi sh and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -107 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## St. Joh ns River n ear DeLand STORET Station 2236000

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.68, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond the forested buffer zone.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of St. John’s River near DeLand (Station 2236000) showing watershed vicinity and land use. The system originates from Blue Cypress Lake, a completely undeveloped system surrounding by forest and wetlands.

Image Removed Du e to Copyright. DOQQ 2004 aerial photograph of St. John’s River near DeLand (Station 2236000) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photographs of St. John’s River ne ar DeLandTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -108 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 4 LDI Score 1.68 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.078 Total Nitrogen Geometric Mean (mg/L) 1.39

The habi tat a ssessment scored in the “Optimal” range. This system is characterized by very low flow, therefore Stream Condition Index sampling was inappropriate according to DEP standard operating procedures. Aquatic vegetation present was typical of sluggish, open systems and there were no nuisance plant issues.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 88.2 % Chlorophycota 2.4 % Cryptophycophyta 0 % Cyanophycota 9.5 Number of Taxa 53

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 53 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal communities) at the St. Johns River n ear D eland. Based on our analy ses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and based upon observations during the site visit, that no adverse algal or aquatic plant issues were present. Thus, not only are the nutrient co ncentrations reflective of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and wildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -109 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Steinhatchee River @ Canal Road

STORET Station 22050083 / STN020C1

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.11, suggesting benign human influences in the watershed. Before the site visit, aerial photographs were examined to assess the watershed land use. Direct observations during the site visit confirmed that the majority of the watershed land use was forest/natural wit h silviculture beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Steinhatchee River (Station 22050083) showing watershed vicinit y and land

use.
Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Steinhatchee River (Station 22050083) showing close -up of sampling site.

Photograph of Steinhatchee River at Canal RoadTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -110 Habitat, Biological and Water Quality Data

Param eter Value Habitat Assessment 151 Hydrologic Score 2 LDI Score 1.11 SCI Score 41 A Total Phosphorus Geometric Mean (mg/L) 0.087 Total Nitrogen Geometric Mean (mg/L) 0.94

The Habitat A ssessment score was in the “Optimal” range. The SCI score was in the “Healthy” category, exhibiting a diverse assemblage of sensitive invertebrates and indicating the site is meeting the Class III designated

use.
Periphyton Community Composition

Algal Community Structure % Composition % Bacillariophyta 60.13 % Chlo rophycota 3.65 % Cryptophycophyta 0 % Cyanophycota 36.21 Number of Taxa 54

This site had an isolated area of Rhizoclonium sp. mats, where the canopy was open and the water was shallow over rocky substrate. Throughout the majority of the site, only sma ll to moderate amounts of algae were present, and this did not affect the inhabitability of the substrates by invertebrates. Of the 5 4 algal taxa observed, the majority of the periphyton community was composed of diatoms.

Overall Reference Site Conclusi on:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at the Steinhatchee River at Canal Rd. Based on our analyses, the Departmen t concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -111 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Steven’s Branch off CR 204 STORET Station 27010070

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.29, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direc t observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the si te.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Steven’s Branch off CR 204 (Station 27010070) showing watershed vicinity and land use. Steven’s Branch originates in an area of pine flatwoods, wetlands and prairie.

Image Removed Due t o Copyright. DOQQ 2004 aerial photograph of Steven’s Branch off CR 204 (Station 27010070) showing close-up of sampling site. An extensive riparian forested buffer is present on both sides of the system.

Photographs of Steven’s Branch off CR 204 (St ation 27010070)Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -112 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 128 Hydrologic Score 3 LDI Score 1.29 SCI Score 76 A Total Phosphorus Geometric Mean (mg/L) 0.120 Total Nitrogen Geometric Mean (mg/L) 1.09

The habit at assessment sc ored in the “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III designation.

Periphyton Communit y Composition

Algal Community Stricture % Composition % Bacillariophyta 75.14 % Chlorophycota 3.39 % Cryptophycophyta 0 % Cyanophycota 21.47 Number of Taxa 66

Minimal periphyton growth was observed at the site, which would allow for optimal substra te use by benthic invertebrate and fish communities. Of the 66 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FD EP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Steven’s Branch off CR 204. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which demonstrate full support of the most sensitive designated 

### use ( propagation and maintenance of a healthy, well -balanced population of fish and

w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -113 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Tosohatchee Creek @ WMA STORET Station OR A331LV

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.50, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Tosohatchee Creek (Station ORA331LV) showing watershed vicinity and land

use . The stream originates in a swampy area adjacent to Tosohatchee Creek State P r eserve.
Image Removed D ue to Copyright. DOQQ 2004 aerial photograph of Tosohatchee Creek (Station ORA331LV) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photograph of Tosohatchee Creek (Station ORA33 1LV).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -114 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 116 Hydrologic Score 1 LDI Score 1.50 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.034 Total Nitrogen Geometric Mean (mg/L) 0.77

The habitat assessmen t sc ored in the high “Suboptimal” range, with water velocity being the lowest scoring parameter. The site was deemed inappropriate for SCI sampling due to the swamp

- like nature of the system and lack of water flow. Vegetation present was typical of minima lly disturbed swamp systems.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 89.00 % Chlorophycota 0.33 % Euglenophycota 0 .33 % Cyanophycota 10.33 Number of Taxa 56

Of the 56 algal taxa identified, the majority of the periphyton community was composed of diatoms. No problematic filamentous algae were observed.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrolog y, algal community, and local land use) at Tosohatchee Creek. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that no adverse algal or aquatic plant issues were present. Thus, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designated 

### use (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -115 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Waccasassa River above SR 24 STORET Station LEV502GS

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.23, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, and field/pasture observed beyond the forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Waccasassa River (Station LEV502GS) showing watershed vicinity and land use. The Wacasassa River originates in an area of hardwood swamp known as Devils Hammock.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of the Waccasassa River (Station LEV502GS) showing close-up of sampling site.

Although the re was some encroachment from a clear cut area near the site, extensive riparian forested wetland buffer is present on both sides for the majority of the system. Note that the forested buffer still exceeded approximately 75 m at minimum, and was generally several hundred m wide.

Photographs of the Waccasassa RiverTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -116 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 138 Hydrologic Score 1 LDI Score 1.23 SCI Score 69 A Total Phosphorus Geometric Mean (mg/L) 0.065 T otal Nitrogen Geometric Mean (mg/L) 0.63

The habitat assessment sc ored in the high “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III designation.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 84.24 % Chlorophycota 0.32 % Cryptophycophyta 0 % Cyanophycota 15.43 Number of Taxa 46

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by benthic invertebrate and fish communities. Of the 46 algal taxa observed, the majority of the periphyton community was composed of diatoms. Although some filamentous algal growth was foun d in a few small areas, it did not affect habitat suitability, as evidenced by the very good SCI score.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, l ocal land use, and algal and macroinvertebrate communities) at the Waccasassa River. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy bio logical assemblages. T hus , not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organ isms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -117 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Withlacoochee River at SR 471 STORET Station WITHLACOORVR1

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index

score of 1.45, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture and field/pasture observed beyond an extensive forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. Aerial photograph of Withlacoochee River at SR 471 (Station WITHLACOORVR1) showing watersh ed vicinity and land use. The Withlacoochee River originates in the Green Swamp, an extensive wetland with considerable areas in public ownership.

Image Removed Due to Copyright. Aerial photograph of Withlacoochee River at SR 471 (Station WITHLACOORVR1) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photograph of Withlacoochee River at SR 471 (Station WITHLACOORVR1).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -118 Habitat, Biological and Water Quality Data

Parameter Va lue Habitat Assessment 119 Hydrologic Score 2 LDI Score 1.45 SCI Score 46 A Total Phosphorus Geometric Mean (mg/L) 0.080 Total Nitrogen Geometric Mean (mg/L) 1.68

The habitat assessment scored in the high “suboptimal” range due to no water velocity . The Stream Condition Index was not performed during this site visit due to low water levels and minimal stream velocity. However, sites both upstream (station 33449) and downstream (station 34401) of the sampling location and within the same WBID receiv ed “healthy” SCI designations in 2007, (51 A and 40 A, respectively) indicating that the water quality is fully supporting the Class III designated use. These scores were averaged for the above table.

Periphyton Community Composition

Algal Community S tricture % Composition % Bacillariophyta 85.95 % Chlorophycota 1.3 % Cryptophycophyta 0 % Cyanophycota 12.1 % Euglenophycota 0.7 Number of Taxa 61

Minimal periphyton growth was observed at the site, which would allow for optimal substrate use by be nthic invertebrate and fish communities. Of the 61 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) Withlacoochee River at SR 471. Based on our analyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biological assemblages. Thus, not only are the nutrient concentrations reflective of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organisms).Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -119 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Withlacoochee River at Stokes Ferry STORET Station 3513

Land Use

This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.82, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the maj ority of the watershed land use was forest/natural, with small areas of silviculture, field/pasture, and residential uses observed beyond a forested riparian buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Cop yright. DOQQ 2004 aerial photograph of Withlacoochee River at Stokes Ferry (Station 3513) showing watershed vicinity and land use. The Withlacoochee River originates in the Green Swamp, a very large wetland with considerable areas in public ownership.

Im age Removed Due to Copyright. DOQQ 2004 aerial photograph of Withlacoochee River at Stokes Ferry (Station 3513) showing close-up of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photographs of the Withlacoochee River at Stokes FerryTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -120 Habitat, Biological and Water Quality Data

Parameter Value Habitat Assessment 153 Hydrologic Score 4 LDI Score 1.82 SCI Score 68 A Total Phosphorus Geometric Mean (mg/L) 0.043 Total Nitrogen Geometric Me an (mg/L) 0.86

The habitat assessment sc ored in the high “Optimal” range, and the Stream Condition Index scored in the “Exceptional” category, indicating an unusually diverse assemblage of sensitive invertebrates, definitively supporting the Class III de signated use.

Periphyton Community Composition

Algal Community Stricture % Composition % Bacillariophyta 90.24 % Chlorophycota 0.7 % Cryptophycophyta 0 % Cyanophycota 9.06 Number of Taxa 40

Moderate periphyton growth was observed at the site; howe ver, substrate availability for benthic invertebrates was not problematic, as evidenced by the “exceptional” SCI score. Of the 40 algal taxa observed, the majority of the periphyton community was composed of diatoms. No problematic filamentous algal growth was found, despite the relatively open, sunny conditions characteristic of the site.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology, local land use, and algal and macroinvertebrate communities) at Withlacoochee River at Stokes Ferry. Based on

our an alyses, the Department concluded that the site was influenced only by very low levels of anthropogenic stressors and that it clearly maintained healthy biol ogical assemblages. T hus
, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota demonstrated to be fully supportive of the designated use (healthy, well balanced populations of aquatic organi sms)

.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -121 PENINSULA NUTRIENT REGION

Nutrient Benchmark Site Summary 

## Withlacoochee River at Trails End STORET Station FL0052000087500

Land Use
This station was considered to be a candidate reference site based on the Landscape Development Intensity Index score of 1.34, suggesting benign human influences in the watershed. During the site visit, aerial photographs were examined and direct observations confirmed that the majority of the watershed land use was forest/natural, with small areas of silviculture, field/pasture, and residential uses observed beyond the forested buffer zone. There are no point source discharges upstream of the site.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Withlacoochee River at Trails End (Station FL0052000087500) showing watershed vicinity and land use. The Withlacoochee River originates in the Green Swamp, a very large wetland with considerable areas in public ownership.

Image Removed Due to Copyright. DOQQ 2004 aerial photograph of Withlacoochee River at Trails End (Station FL0052000087500) showing closeup of sampling site. An extensive riparian forested wetland buffer is present on both sides of the system.

Photographs of Withlacoochee River at Trails EndTechnical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A5 -122 Habitat, Biological and Water Qualit y Data

Parameter Value Habitat Assessment 118 Hydrologic Score 2 LDI Score 1.34 SCI Score NA Total Phosphorus Geometric Mean (mg/L) 0.059 Total Nitrogen Geometric Mean (mg/L) 1.01

The habitat assessment sc ored in the high “Suboptimal” range. The SCI was not performed due to the morphology of the system in this area (lake -like), which when coupled with low water levels, resulted in the system having no perceptible water velocity. Typical aquatic plants observed at the site included Taxodium distichum, Hydrocotyle sp ., Panicum hemitomon, Myriophyllum heterophyllum, and Polygonum sp., indicating healthy conditions.

Qualitative Periphyton Sampling was not performed at this site, however minimal periphyton growth was observed, which would allow for opt imal substrate use by benthic invertebrate and fish communities. No problematic filamentous algal growth was found, despite the relatively open, sunny conditions characteristic of the site.

Overall Reference Site Conclusion:
Experienced FDEP scientists conducted a series of observations and measurements (habitat assessment, hydrology and local land use) at Withlacoochee River at Trails End. Based on our an alyses, the Department concluded that the site was influenced only by very low levels of anthropo genic stressors and that no adverse algal or aquatic plant issues were present. T hus, not only are the nutrient concentrations reflect ive of minimally disturbed conditions, they are associated with biota which suggest support of the most sensitive designa ted 

### use (propagation and maintenance of a healthy, well -balanced population of fish and w ildlife).

# FDEP’s Site Inform ation and Taxa Lists for Stream Benchmark Site Samples

(Appendix 3-D from USEPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters . United States Environm ental Protection Agency, Office of Water, Office of Science and Technol ogy, Health and Ecological Criteria Division, Washington, DC.)If you have a disability and are unable to read this spreadsheet, please contact Eric Shaw at Eric.Shaw@dep.state.fl.us, and you will be provided with an accessibleversion.SCI2004 taxa lists are presented by sample.SCI2007 taxa lists are presented by replicate.Both taxa matrices are collapsed using collapsing rules in FDEP SOP LT7000, and metrics and indices are calculated on collapsed taxa lists.SCI2004 metrics and final scores are calculated and presented by sample.SCI2007 metrics are calculated and presented by replicate. The final score is calculated and presented as an average for the two replicates.Three periphyton samples were analyzed by older methodology where the number of diatomvalvesidentified on the fixed slide was equal to 2x the numberof diatomfrustulescounted in the wet material. For data entry, wet ID and diatom ID were combined. Diatom valve counts from the fixed slide weredivided by two (to represent a frustule) and entered with the wet ID's. The only exception is that a valve count of 1 was entered as a frustule count of 1.These three periphyton samples had to be retrieved separately for taxa lists and are presented inPeri Data Wet & Diatom Combined.Metrics for these three periphyton samples are slightly different in that dominants, number of taxa, etc. are based on the sample as a whole andnot separately for diatoms vs. soft algae (wet ID).All other periphyton samples in this file were analyzed by the current method were the number of diatom valves identified on the fixed slide is 500 +/-regardless of the number of diatom frustules counted in the wet ID. Diatom data is entered separately from wet ID data, and metrics are calculated oneach data set (i.e. DominantDiatomOnlyPct, DominantWetAlgaeOnlyPct, etc.). Taxa lists are also retrieved and presented separately.As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 A blabesmyia mallochi -----1 A blabesmyia rhamphe grp.

------ A centrella - - - -37 A centrella parvula

------ A cerpenna pygmaea

- 2 1

- - - A croneuria

------ A croneuria abnormis

------ A croneuria lycorias

------ A gnetina annulipes

------ A llonais inaequalis

------ A mnicola

------ A mphipoda

------ A ncylidae ----3- A ncyronyx variegatus

------ A nisocentropus pyraloides

------ A pedilum

------ A rgia

- - - 1

- 2 A rgia fumipennis

------ A rgia tibialis 1 4

---- A rrenurus

------ A

sioplax

------ A therix

------ A tractides 1

- 1 1

- - A trichopogon

------ A ulodrilus pigueti

------ Baetidae

------ Baetis intercalaris 6

----- Baetisca becki

------ Baetisca rogersi

------ Beardius

------ Berosus

------ Bivalvia

------ Boyeria vinosa 1 2

- 1

- - Brachycentrus chelatus

------ Brachycentrus numerosus

------ Branchiobdellidae

------ Bratislavia unidentata

------ Brillia

------ Caecidotea

------ Caenis

------ Caenis diminuta 2

----- Caenis eglinensis

------ Caenis hilaris

------ Callibaetis

------ Callibaetis floridanus

------ Calopterygidae

------ Calopteryx

- - - 1

- - Cambaridae -----1 Campeloma

------ Cecidomyiidae

------As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Celina

------ Centroptilum triangulifer

------ Ceraclea

------ Ceratopogonidae - - - -12 Cernotina

------ Chauliodes

------ Cheumatopsyche 3 4 31 29

- - Chimarra

- - - 1

- - Chironomus

------ Choroterpes basalis

------ Chrysomelidae

------ Cladotanytarsus

------ Cladotanytarsus cf. daviesi - - - -44 Cladotanytarsus sp. d epler -----1 Cladotanytarsus sp. f epler

------ Cladotanytarsus viridiventris

------ Clathrosperchon

- - - 1

- - Climacia areolaris

------ Clinotanypus

------ Coenagrionidae

------ Corbicula fluminea

- 1

- - 6 1 Corydalidae

------ Corydalus cornutus

------ Corynoneura

------ Crangonyx

------ Cricotopus

------ Cricotopus bicinctus ----3- Cricotopus or orthocladius - - - -12 Cryptochironomus -----1 Cryptotendipes -----1 Curculionidae

------ Cyrnellus fraternus

------ Dasyhelea

------ Dero

------ Dero digitata complex

------ Dero flabelliger

------ Dero furcata

------ Dero pectinata

------ Desmopachria

------ Desserobdella phalera

------ Dicrotendipes

------ Dicrotendipes modestus

------ Dicrotendipes neomodestus - - - -1 3 7 Dicrotendipes simpsoni

------ Dicrotendipes sp. a epler

------ Dineutus

------ Diplectrona

------ Djalmabatista pulcher

------ Dryopidae

------ Dubiraphia vittata

- 1

- - 1

-As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Dytiscidae

------ Eclipidrilus

------ Eclipidrilus palustris

------ Ectopria

------ Ectopria thoracica

------ Einfeldia sp. a epler

------ Elimia

------ Elmidae

------ Enallagma

- - 2

- - - Enallagma coecum

------ Enchytraeidae 1

----- Endotribelos hesperium

------ Enochrus

------ Epicordulia princeps regina

------ Eurylophella doris

------ Ferrissia 3

----- Gastropoda

------ Geayia

------ Gerridae

------ Glossiphoniidae

------ Goeldichironomus

------ Goeldichironomus cf. natans

------ Goeldichironomus holoprasinus

------ Gomphidae

------ Gomphus 1 1

---- Gonielmis dietrichi

------ Gyrinidae

------ Gyrinus

------ Haemonais waldvogeli

------ Hagenius brevistylus

------ Haitia

- - 1

- - - Hayesomyia senata

------ Helichus

------ Helichus lithophilus

------ Helobdella stagnalis -----3 Helobdella triserialis

------ Hemerodromia 1

----- Heptageniidae

------ Hetaerina 1 3

---- Hetaerina titia

------ Hexagenia

------ Hexatoma

------ Hirudinea

------ Hyalella azteca - - - -36 Hydraena

------ Hydrobiidae ----2- Hydrodroma

------ Hydrometra

------ Hydrophilidae

------ Hydropsyche

- - 5

- 6 1As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Hydropsyche incommoda

------ Hydropsyche mississippiensis

------ Hydroptila - - - -1 3 8 Hydrovatus

------ Hygrobates

- - 1

- - - Ischnura

------ Isonychia

------ Kiefferulus

------ Koenikea

------ Krendowskia

------ Labrundinia

------ Labrundinia becki

------ Labrundinia johannseni

------ Labrundinia pilosella

- 1

---- Labrundinia sp. 3 nr. virescens

------ Laccophilus

------ Laevapex

- 2

---- Larsia

------ Larsia decolorata

------ Lauterborniella agrayloides

------ Lebertia - - - -11 Lepidoptera

------ Lepidostoma

------ Leptophlebiidae ----1- Leuctra

------ Libellulidae

------ Limnesia

------ Limnodrilus hoffmeisteri -----3 Lioporeus

------ Lirceus 15 14

---- Lopescladius

------ Lumbriculus variegatus

------ Lymnaeidae ----1- Lype diversa

------ Maccaffertium

------ Maccaffertium exiguum 8 6

- - 1

- Maccaffertium smithae 5954- - Macromia 1

----- Macromia illinoiensis georgina

------ Macronychus glabratus 3212-1 Macrostemum carolina

------ Mayatrichia ayama ----2- Megistocera

------ Mesonoterus

------ Mesovelia

------ Micrasema

------ Microcylloepus pusillus 4 9 13 25

- - Micromenetus 6 2

---- Micromenetus dilatatus avus

------ Micropsectra

------As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Microtendipes

------ Microtendipes pedellus grp.

------ Microtendipes rydalensis grp.

------ Microvelia

------ Mideopsis

- - 1

- - - Momonia

------ Monopelopia

------ Mooreobdella

------ Naididae

------ Nais communis complex

------ Nais(animal)

------ Nanocladius ----1- Nectopsyche

------ Nectopsyche exquisita

- 2

- - - 1 Nectopsyche pavida

------ Neoephemera

------ Neoperla

------ Neoplasta

------ Neoplea

------ Neoporus

------ Neotrichia

- - 2231 Neureclipsis 1

----- Neureclipsis crepuscularis

------ Neurocordulia

------ Neurocordulia alabamensis

- - - 1

- - Nigronia

------ Nigronia serricornis

------ Nilotanypus americanus

------ Nilotanypus fimbriatus

------ Nilothauma

------ Notomicrus nanulus

------ Nyctiophylax

------ Oecetis ----2- Oecetis avara

------ Oecetis cinerascens

- 2

---- Oecetis georgia

- - - 3

- - Oecetis sphyra/morsei

------ Ora/scirtes

------ Oribatida

------ Ormosia

------ Orthocladius annectens

- - 1

- - - Orthotrichia

------ Oxus

------ Oxyethira -----1 Pachydiplax longipennis

------ Palaemonetes -----1 Palpomyia/bezzia grp.

------ Parachaetocladius abnobaeus

------ Parachironomus

------ Parachironomus chaetoalus complex

------As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Parachironomus supparilis

------ Paracladopelma

------ Paracymus

------ Parakiefferiella sp. b epler

------ Parakiefferiella sp. f epler -----2 Paralauterborniella nigrohalterale

------ Paraleptophlebia volitans

- - 3 6

- - Paramerina

------ Parametriocnemus

------ Paratanytarsus

------ Paratanytarsus dissimilis

------ Paratanytarsus quadratus

------ Paratendipes subaequalis

------ Pelonomus obscurus

------ Peltodytes

------ Peltodytes dietrichi

------ Pentaneura inconspicua

- - 1

- 1 2 Perlesta placida complex

------ Perlinella drymo

------ Phaenopsectra flavipes

------ Phylocentropus

------ Physa

------ Physidae

------ Pilaria

------ Placobdella

------ Planorbella

------ Planorbidae ----1- Platyhelminthes 2 1

---- Plauditus

------ Plauditus bimaculatus

------ Plecoptera

------ Polycentropodidae

------ Polycentropus

- 2

---- Polypedilum aviceps

------ Polypedilum fallax

------ Polypedilum flavum 18 9 27 31 10 9 Polypedilum halterale grp. -----1 Polypedilum illinoense grp.

- - 1

- - - Polypedilum scalaenum grp. 1

- - - 11 19 Polypedilum sp. a epler

------ Polypedilum trigonus

------ Polypedilum tritum

------ Potthastia

------ Potthastia longimana

------ Pristina

------ Procambarus

------ Procladius

------ Procloeon

------ Progomphus

------ Progomphus obscurus

------As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Promoresia tardella

------ Prostoma

------ Psectrocladius

------ Pseudochironomus

------ Pseudocloeon

- 1

- - 1

- Pseudocloeon ephippiatum

------ Pseudocloeon frondale

- - 3 3

- - Pseudocloeon propinquum

------ Pseudolimnophila

------ Psychodidae

------ Pteronarcys dorsata

------ Pyralidae

------ Rheocricotopus robacki

- 2 7 6

- 1 Rheotanytarsus

------ Rheotanytarsus exiguus grp.

- - 2 1

- - Rheotanytarsus pellucidus

- 1 3 5

- - Rhyncholimnochares

------ Robackia clavige r

------ Serratella deficiens

------ Sialis

------ Simulium 68 66

- - - 1 Simulium jonesi

- - 24 18

- - Sisyra

------ Slavina appendiculata

------ Sperchon

------ Sperchonopsis

------ Sperchopsis

------ Sperchopsis tessellatus

------ Sphaeriidae(mollusca) 2 7

---- Spirosperma

------ Staphylinidae

------ Stelechomyia perpulchra

------ Stempellina

------ Stempellinella

------ Stempellinella fimbriata

------ Stempellinella leptocelloides

------ Stenacron

- 1

---- Stenacron interpunctatum

------ Stenelmis

- - 2384 Stenochironomus

- 1 1

- - - Stenus

------ Stephensoniana trivandrana

------ Stratiomyidae

------ Sympetrum

------ Tabanidae

------ Taeniopteryx

------ Tanytarsus

------ Tanytarsus sp. a epler - - - -15 Tanytarsus sp. c epler - - - -57 Tanytarsus sp. d epler

- - 3

- - -As Calculated / STORET station -> LEV502GS LEV502GS PUT308GS PUT308GS 3513 3513 Sample date -> 2/21/2007 2/21/2007 2/26/2007 2/26/2007 2/20/2007 2/20/2007

Taxon Name / SampleID/BenthID -> 57322/36904/ Rep 1 57322/36904/ Rep 2 57431/36987/ Rep 1 57431/36987/ Rep 2 57324/36989/ Rep 1 57324/36989/ Rep 2 Tanytarsus sp. e epler

------ Tanytarsus sp. f epler

------ Tanytarsus sp. l epler

- - 1

- - - Tanytarsus sp. m epler

- - 2 2

- - Tanytarsus sp. n epler

------ Tanytarsus sp. o epler

------ Tanytarsus sp. p epler - - - -21 Tanytarsus sp. s epler

------ Tanytarsus sp. t epler ----1- Tanytarsus sp. y epler - - - -31 Thienemanniella

------ Thienemanniella lobapodema

------ Thienemanniella similis

------ Thienemanniella xena

- - - 1 1

- Thienemannimyia grp.

- - 1

- - - Tipula

------ Tipula caloptera

------ Tipulidae

------ Torrenticola

------ Triaenodes

- 1

- 1

- - Triaenodes ignitus

------ Triaenodes perna/helo

------ Tribelos

------ Tribelos fuscicornis

------ Tribelos jucundum

------ Tricorythodes albilineatus - - - -3 1 4 2 Tubificidae ----1- Tvetenia

------ Tvetenia vitracies

------ Vertigo

------ Viviparus georgianus

------ Xenochironomus xenolabis

- - - 1

- - Xestochironomus 2

----- Xylotopus pa r

------ Zavreliella marmorata

------ Zavrelimyia sp. a epler

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2 119421 211- - -

------

------ - - 91 0- -

------

------

------

------

------

------

------ ---1-- ---3--

------

------ 2----- - -11- -

------

------

------

------

------ --2--1

------

------

------ 11- - - -

------

------

------

------

------

------

------

------

------

------ -137- - --1-4-

------

------

------

------

------

------

------

------ ---2--

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------

------ 1 1 1 1- - - -

------

------ 1-95- - -1----

------

------

------ - - - -11

------

------

------

------

------

------

------ -----1 - -23- -

------

------

------

------ 1- - -21 - -11- -

------

------

------

------ - - - -21

------

------

------

------

------

------

------

------

------

------ - - - -3 3 2 5

------

------

------

------ --1---

------ -1----As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------

------

------

------

------

------

------ --1---

------

------

------

------

------ 1311- -

------

------

------

------

------

------

------

------

------

------ 54- - - -

------

------

------

------ - -32- -

------

------

------

------

------ 1----4 -1----

------

------

------

------

------ - -73- -

------ --2---

------

------

------ 43- -32As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------ 26 30

- - 5 5

------ --2---

------

------

------

------

------ -1---- --3---

------

------

------

------

------

------

------

------ 1-----

------

------ 1 1- 1 - - -

------

------

------

------

------

------

------

------

------

------ --3-1- -----2

------

------

------ --1--- - - - -23

------ ---1--

------

------ 11- - - - - -54- - - - 41 4- -

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------ 11- - - -

------

------

------

------

------

------

------

------ ----1-

------ -1----

------

------

------

------

------ ---1--

------

------

------

------

------

------

------

------

------ -----3

------

------ ----1-

------

------ --1---

------ 1-----

------

------ - - - -11

------

------ --2---

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------ 1-----

------

------

------

------ ---1--

------ 11- - - -

------

------

------

------

------

------

------

------ -2----

------ --1--- --1---

------

------

------ ---1--

------

------ - -12- -

------

------ 1-----

------ ---1--

------ ---4--

- - 21 10 7 1

------

- - 10 14

- - 4-23- -

------

------

------

------

------ - -12- -

------ --1---

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------ - - - -69 3

0 3 7- - - - 3-3231

------

------

------

------

------

------

------ -11- - -

------ 1-27- -

------

------

------

------

------ ---3--

------

------

------

------ 42- - - -

------ 1----- --1---

------

------

------

------

------ --1---

------

------

------ 10 10 5 2 6

-

------

------

------

------

------

------

------

------ ---7-- - -11- - 4 2 - - 91 8As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 3545 3545 21202 21202 3535 3535 3/26/2007 3/26/2007 3/1/2007 3/1/2007 10/10/2006 10/10/2006 59773/38075/ Rep 1 59773/38075/ Rep 2 60301/38292/ Rep 1 60301/38292/ Rep 2 60308/38307/ Rep 1 60308/38307/ Rep 2

------

------ 342- - -

- - 15 9

- -

------

------ - -11- -

------ ---1--

------ --1---

------

------

------ 12 17 1 5 17 7

------

------

------

------

------

------

------

------

------

------

------ 21- - - -

------ 9 6

- - 39 54 ---2--

------ 1--1--

------

------

------ ---1--As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2 --2--- --1--1

------

------ ---2--

------

------

------

------

------

------

------ 1- -142 -1----

------

------ ---3--

------

------

------

------

------ --1-1-

------

------ 2----- - -12- -

------

------

------

------

------ 1-----

------

------

------

------

------ - -14- - 8 11 - - - -

------ - - - - 14 26 ---1--

------

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------

------

------ - - -122

------

------

- - 12 10

- - - - - -11

------

------

------

------ - - - -22

------ - - - -53 - - - -1 6 1 0

------

------

------

------ 117511

------ ----1- ---1--

------

------ ---3-- 1 2

- - 10 17 --1-2- --1---

------

------

------ ---2--

------

------

------

------

------

------

------

------ - - - -2 5 1 4

------

------ -1----

------

------

------ 2123- -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------

------

------

------

------

------ 2

9 1 9- - - -

------ ---5-- --4---

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------

------ 1 7 1 4- - - -

------

------

------

------

------ 2----- -4----

------

------ ----1-

------ ---1-- 32- - - -

------

------

------

------

------

- 4 3 2 11 12As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------

------ 4 6 1 21 4 1 2

------ ---1--

------ -----1

------

------

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------

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------

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------

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------

------

------

------

------

------ 5 6 11 17

- 1 ---3-- --1--- 1-----As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------

------ 1 03 - - - -

------ --1---

------

------

------

------

------

------

------

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------

------

------

------

------

------ - -331-

------

------

------

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------

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------

------

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------

------

------ - - - -21

------

------

------

------

------

------ --1---

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------

------

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------ - - - -12 - -1113

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------ -2----

------ - - - -45

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2

------ ---1--

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- 2

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 3546 3546 24010002 24010002 3549 3549 5/15/2007 5/15/2007 5/16/2007 5/16/2007 9/19/2007 9/19/2007 60639/38485/ Rep 1 60639/38485/ Rep 2 60751/38544/ Rep 1 60751/38544/ Rep 2 62733/39186/ Rep 1 62733/39186/ Rep 2 --1---

------ -11- - - 12-9- -

------

------

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------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2 1- - -11

------

------

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------

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------

------

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------

------

------ - - - -15

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

------

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Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

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------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

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Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2 1-----

------

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Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

------

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6 2 0- - - - - -32- -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 3513 3513 3535 3535 LIB104LV LIB104LV 11/7/2007 11/7/2007 12/12/2007 12/12/2007 6/10/2008 6/10/2008 63694/39707/ Rep 1 63694/39707/ Rep 2 64191/40004/ Rep 1 64191/40004/ Rep 2 65025/40260/ Rep 1 65025/40260/ Rep 2

------

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------

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Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2 1----1

------

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Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------ -7---- -----1

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------ -----1As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------ - - -111

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------

------

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------

------ - -9356As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------

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------

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------

------

------

------

------

------ -14815

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------

------

------

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------

------

------

------

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------ 3----- -1----

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------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------

------

------

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------

------

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------ 22- - - - 41- - - - -----1As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 22020093 22020093 GAD106GS GAD106GS NUTREF001 NUTREF001 6/13/2008 6/13/2008 6/13/2008 6/13/2008 6/10/2008 6/10/2008 65026/40261/ Rep 1 65026/40261/ Rep 2 65028/40263/ Rep 1 65028/40263/ Rep 2 65029/40264/ Rep 1 65029/40264/ Rep 2

------

------ ----1- 1-1-11 - -11- -

------

------

------

------

------

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------

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- 3 6 10 1

------

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------ - -3677

------

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------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2 - - -111 -----1

------

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------ - -31- - - -35- -

------ -1----As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------

------

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------

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------ 112- -2As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------ 1-----

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------

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------

------ -----1 5 2 5 43 7 5 4As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------

------ 32 38 6 11 17 26

------

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------ - -54- - ---2--

------

------

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------

------

------

------ - -2-43

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------ 12- - - -

------

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------

------

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------

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------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------

------

------

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------

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------

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2 --2---

------ -1---- ----5- 44- -41

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------

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------ 11- - - - 1---1- - -22- -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 3545 3545 32030023 32030023 21010008 21010008 7/9/2008 7/9/2008 7/24/2008 7/24/2008 7/16/2008 7/16/2008 65291/40343/ Rep 1 65291/40343/ Rep 2 65292/40344/ Rep 1 65292/40344/ Rep 2 65293/40345/ Rep 1 65293/40345/ Rep 2

------

------ -1----

------

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------

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------

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------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2 45- -1- 11-2- -

------

------

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------

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------

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------ - - - -65

------

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------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------

------

------ -11- - - ----1-

------ 34- -2-

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------

------ -2- -52As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------

------

------

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------

------

------ 33- - - -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

- - 87 68

- -

- - 10 15

- - 1 46 9 9 1 5

------

------

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------

------

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------

------

------ 231-99

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------ -1----

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 3546 3546 3549 3549 22050083 22050083 7/9/2008 7/9/2008 7/10/2008 7/10/2008 8/12/2008 8/12/2008 65296/40348/ Rep 1 65296/40348/ Rep 2 65316/40349/ Rep 1 65316/40349/ Rep 2 65379/40364/ Rep 1 65379/40364/ Rep 2

------

------ 11- - - -

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2 ---3-2 511- - -

------

------

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- - 47 36

------

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------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------

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5 2 9- - - -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------

------

------

------

------

------

------ - - - -33

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------

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Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------ - -11- -

------

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------

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------

------

------

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------ - -36- -

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------ --1---

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------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------

------

------

------

------

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------

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------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------

------

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------

------

------

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------

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------ ---1--As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 22050040 22050040 CLA246GS CLA246GS 23010464 23010464 8/12/2008 8/12/2008 5/28/2008 5/28/2008 5/7/2008 5/7/2008 65382/40367/ Rep 1 65382/40367/ Rep 2 65250/40368/ Rep 1 65250/40368/ Rep 2 64546/40384/ Rep 1 64546/40384/ Rep 2

------

------ ---1-- --2---

------ - -21- -

------ - -11- - 11- -21

------

------

------

------

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------

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------

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2 2 -1 671 5 4 2----1

------

------ ----1-

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

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Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

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- - 1

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------ 10 11

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Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

------

------

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Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

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Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler WAK158LR WAK158LR BAK208GS BAK208GS CLA246GS CLA246GS 6/19/2008 6/19/2008 6/18/2008 6/18/2008 10/28/2008 10/28/2008 65414/40405/ Rep 1 65414/40405/ Rep 2 65306/40406/ Rep 1 65306/40406/ Rep 2 65636/40430/ Rep 1 65636/40430/ Rep 2

------

------ -24462 22- -41

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Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2 -----1 ----1-

------

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------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2 ---3--

------

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Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------ ---1--

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Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------

------

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Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------

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Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------

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Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------

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------

------ ----1-As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler UNI234LV UNI234LV 23010464 23010464 19010006 19010006 10/16/2008 10/16/2008 10/8/2008 10/8/2008 11/12/2008 11/12/2008 65637/40431/ Rep 1 65637/40431/ Rep 2 65567/40448/ Rep 1 65567/40448/ Rep 2 65731/40476/ Rep 1 65731/40476/ Rep 2

------

- - 21 22

- - 32- - -3

------

------ 1-----

------

------ - -33- -

------

------

------

------

------ 79- -11

------

------

------

------ 1----3

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------ - - - -52 1 3 1 0- - - -

------ -2----

------

------

------

------ -----1

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2 2- -132

------

------

------ 21- - - -

------

------

------

------

------

------

------ 1133- - 412- - -

------ 1---1- 4 5 11 5 11 14

------

------ 12- -22

------

------

------

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------

------

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------

------

------

------

------

------ 112155

------

------

------

------

------

------ -1---- 2-38- - -11- -3

------ -----1As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2

------

------

------

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------ 1 26 6 5 1 1 6682- - ----1-

------

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------

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------

------

------

------

------

------ --2---

------ - - - -11

------ -1-142As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2 -1---- 3----- --3---

------ - - - -13

------

------

------ - -2111

------

------

------

------

------ 822282

------ ----1-

------ ----1-

------

------

------

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------

------

------

------

------

------

------

------

------

------

------

------ 222111

------

------

------

------

------

------

------

------

------

------

------

------ ---2--As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2

------

------ 16- -1-

------

------

------

------

------

------

------

------ -1----

------

------

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------

------

------

------

------

------

------

------ 2

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------

------

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------

------

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------

------

------ 1-----

------ 1-----

------

------ --1---

------ 24 18 9 12 11 10

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2 -----1 11-1- -

------

------ - - - -42 - - - -46

------

------

------

------

------

------ -4---- - - - -1 4 1 5 ----1-

------ 44- - - -

------

------ - -11- - 3213-5

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------ - -11- - 2346-3

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------

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------

------

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------ -12-31

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2

------

------

------ ----1-

------

------

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------ 21 27 15 14 2 1

------ - -4111 --1---

------

------

------

------

------

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------

------

------ --2--3

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2

------

------

------ - - - - 31 0 45- - - -

------

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------ 8 2 71 4- 4 221182

------

------

------

------

------ - - - -51

------ ----1- -----1 - -2131As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler CLA254LR CLA254LR CLA243LV CLA243LV WAK158LR WAK158LR 11/6/2008 11/6/2008 11/6/2008 11/6/2008 11/13/2008 11/13/2008 65736/40479/ Rep 1 65736/40479/ Rep 2 65737/40481/ Rep 1 65737/40481/ Rep 2 65715/40524/ Rep 1 65715/40524/ Rep 2

------

------ 1- - -52 1-----

------

------

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------

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------

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------ 5 10 20 25 10 9

------

------

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------

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------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2 ---1-1

------

------

------

------

------

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------

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------

------ -2---- - -64- - - -12- -

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------

------

------

------

------

------ -1---- - -22- -

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------ -544- -

------

------

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------

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------

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------

------

------

------

------

------ - -11- -

------ - -11- -

------ ----1-As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

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------

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------

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------

------

------

------

------ -----2

------ -1----

------

------

------ 7422- -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

------

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------

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------

------

------

------

------

------ 5 5 4 52 5 2 2

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

------

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------

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------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

------

------

------

------

------

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------

------

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------

------

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- 14

- 4 2 --1--- - - -31-

------

------

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------

------

------ 33- - - -

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

------

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------

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------

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------ 6 3

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------

------

------

------

------ 3 2 26 37

- -

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler NUTREF001 NUTREF001 GAD106GS GAD106GS GLA630GS GLA630GS 11/20/2008 11/20/2008 11/20/2008 11/20/2008 12/16/2008 12/16/2008 65739/40534/ Rep 1 65739/40534/ Rep 2 65741/40537/ Rep 1 65741/40537/ Rep 2 65891/40544/ Rep 1 65891/40544/ Rep 2

------

------

------ -231- -

------

------

------

------

------

------

------

------

------

------ 2-16- -

------

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------ 3344- -

------

------

------

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------

------

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------

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2 112-13 - - - -14

------

------ 3-3238

------

------

------

------

------

------

------ 233411

------

------

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------

------

------

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------ - - - -11As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------

------ - - - -23

------

------ 6855-1

------

------

------

------

------

------

------

------

------

------

------

------ - -111- 4-41- -

------

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------

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------

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------

------

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------

------ ----1- - -11- -

- 1 13 15 7 14As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------ ---1--

------

------

------

------

------

------

------

------

------

------

------ - -2-25

------

------

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------

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------

------

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------

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------

------

------ 8 1 3- - - -As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------

------

------ ----1-

------

------

------

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------

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------

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------

------

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------

------

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9 3 1- - - -

------

------

------

------

------

------

------

------

------

------ 7 3 26 23 2 3 1-1--3

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------ -----2

------ -----1

------

------

------

------

------

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------

------

------

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------

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------

------

------

------

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------

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------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------

------

------

------

------

------

------

------ - - - -4 1 1 6

------

------

------

------

------

------ -211-1

------

------

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------

------

------ - -1-11

------

------

------

------

------

------ ---2-- 50 56 45 39

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- 2 4 12 1

------

------

------

------

------

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------

------

------ - - - -64 6113- -

------

------

------

------

------

------ 21- - - -

------ 18 17 14 13 18 17 --1---

------

------

------

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------

------

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------

------

------

------ --3--- ---2-- 26852- 1- -211

------

------

------

------

------

------

------

------ -1- -11

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 24010002 24010002 273116508208 152 273116508208 152 22050040 22050040 12/17/2008 12/17/2008 12/17/2008 12/17/2008 1/14/2009 1/14/2009 65900/40550/ Rep 1 65900/40550/ Rep 2 65907/40552/ Rep 1 65907/40552/ Rep 2 65932/40564/ Rep 1 65932/40564/ Rep 2

------

------

------ - - -12-

------

------

------ -1---- - - -11-

------

------ ----1-

------

------

------

------

------

------

------

------ - -11- - - -23- -

------ - - -111 ----1- ----1-

------

------

------

------

------

------

------

------

------

------As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class - -22 A rthropoda Insecta 1--- A rthropoda Insecta --1- A rthropoda Insecta ---1 A rthropoda Insecta - -52 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta 8 4

- - Mollusca Gastropoda

---- A rthropoda Crustacea 1 4

- - Mollusca Gastropoda 32- - A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 21- - A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida - -11 A rthropoda Insecta

---- A nnelida Oligochaeta 21- - A rthropoda Insecta - -22 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 1--- A rthropoda Insecta

---- Mollusca Bivalvia -1-- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A rthropoda Insecta -13- A rthropoda Crustacea 3334 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Crustacea

---- Mollusca Gastropoda

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 26- - A rthropoda Insecta

---- A rthropoda Insecta 9964 A rthropoda Insecta ---2 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -1-- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta ---3 A rthropoda Insecta 15 8

- - Mollusca Bivalvia

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Crustacea

---- A rthropoda Insecta --2- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 1--- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta ---1 A rthropoda Insecta

---- A nnelida Hirudinea

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 94- - A rthropoda Insecta

---- A rthropoda Insecta 7712 A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta -1-- A nnelida Oligochaeta --1- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

- - 4 1 Mollusca Gastropoda

---- A rthropoda Insecta 12- - A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta -2-- A rthropoda Insecta -1-- A rthropoda Insecta

---- Mollusca Gastropoda

---- Mollusca Gastropoda

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A nnelida Hirudinea

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -2-- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta - -22 A rthropoda Insecta

---- A nnelida Oligochaeta 1--- A rthropoda Insecta

---- Mollusca Gastropoda

---- A rthropoda Insecta ---1 A rthropoda Insecta 2--- A rthropoda Insecta

---- A nnelida Hirudinea 1--- A nnelida Hirudinea 12- - A rthropoda Insecta 11- - A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Hirudinea

- - 10 14 A rthropoda Crustacea

---- A rthropoda Insecta

- - 2 2 Mollusca Gastropoda

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

- - 10 11 A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

---- A rthropoda Insecta -1-1 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- Mollusca Gastropoda

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -1-- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta 3357 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Crustacea 1--- A rthropoda Insecta

---- A nnelida Oligochaeta

---- Mollusca Gastropoda

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 1--- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 24 28 12 6 A rthropoda Insecta 8 3

- - Mollusca Gastropoda

---- Mollusca Gastropoda

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda A rachnida

---- A rthropoda Insecta ---1 A nnelida Hirudinea

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta 11- - A rthropoda Insecta 2--- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -1-- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta ---1 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta ---1 A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida ---1 A rthropoda Insecta

---- A rthropoda Insecta 22- - A rthropoda Crustacea 14- - A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 2-85 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- Mollusca Gastropoda

---- Mollusca Gastropoda

---- A rthropoda Insecta

---- A nnelida Hirudinea

---- Mollusca Gastropoda

---- Mollusca Gastropoda

- - - 2 Platyhelminthes

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -3-- A rthropoda Insecta 3 1 36 38 A rthropoda Insecta 1--- A rthropoda Insecta - -42 A rthropoda Insecta - -13 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A rthropoda Crustacea 13- - A rthropoda Insecta

---- A rthropoda Insecta -1-- A rthropoda Insecta

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

- 1

- - Nemertea Enopla

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta ---1 A rthropoda Insecta - -55 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 1553 A rthropoda Insecta - -34 A rthropoda Insecta

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta - -66 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A rthropoda A rachnida

---- A rthropoda A rachnida

---- A rthropoda Insecta

---- A rthropoda Insecta 7 8

- - Mollusca Bivalvia

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --1- A rthropoda Insecta

---- A rthropoda Insecta --2- A rthropoda Insecta

---- A rthropoda Insecta 7 1 1- 2 A rthropoda Insecta - -41 A rthropoda Insecta

---- A rthropoda Insecta

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta 2--- A rthropoda Insecta

---- A rthropoda Insecta - -14 A rthropoda Insecta

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler 21202 21202 22050083 22050083 5/1/2008 5/1/2008 1/14/2009 1/14/2009 66028/40571/ Rep 1 66028/40571/ Rep 2 65931/40577/ Rep 1 65931/40577/ Rep 2 Phylum Class

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --2- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta --1- A rthropoda Insecta ---1 A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda A rachnida 21- - A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda Insecta -112 A rthropoda Insecta

---- A rthropoda Insecta - -31 A rthropoda Insecta

---- A nnelida Oligochaeta

---- A rthropoda Insecta

---- A rthropoda Insecta

---- Mollusca Gastropoda

---- Mollusca Gastropoda

---- A rthropoda Insecta 11- - A rthropoda Insecta -1-- A rthropoda Insecta

---- A rthropoda Insecta

---- A rthropoda InsectaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae Order Family Genus Diptera Chironomidae A blabesmyia Diptera Chironomidae A blabesmyia Ephemeroptera Baetidae A centrella Ephemeroptera Baetidae A centrella Ephemeroptera Baetidae A cerpenna Plecoptera Perlidae A croneuria Plecoptera Perlidae A croneuria Plecoptera Perlidae A croneuria Plecoptera Perlidae A gnetina Haplotaxida Naididae A llonais Mesogastropoda Bithyniidae A mnicola A mphipoda Basommatophora A ncylidae Coleoptera Elmidae A ncyronyx Trichoptera Calamoceratidae A nisocentropus Diptera Chironomidae A pedilum Odonata Coenagrionidae A rgia Odonata Coenagrionidae A rgia Odonata Coenagrionidae A rgia A cariformes A rrenuridae A rrenurus Ephemeroptera Leptohyphidae A sioplax Diptera A thericidae A therix A cariformes Hygrobatidae A tractides Diptera Ceratopogonidae A trichopogon Haplotaxida Tubificidae A ulodrilus Ephemeroptera Baetidae Ephemeroptera Baetidae Baetis Ephemeroptera Baetiscidae Baetisca Ephemeroptera Baetiscidae Baetisca Diptera Chironomidae Beardius Coleoptera Hydrophilidae Berosus

Odonata A eshnidae Boyeria Trichoptera Brachycentridae Brachycentrus Trichoptera Brachycentridae Brachycentrus Branchiobdellida Branchiobdellidae Haplotaxida Naididae Bratislavia Diptera Chironomidae Brillia Isopoda A sellidae Caecidotea Ephemeroptera Caenidae Caenis Ephemeroptera Caenidae Caenis Ephemeroptera Caenidae Caenis Ephemeroptera Caenidae Caenis Ephemeroptera Baetidae Callibaetis Ephemeroptera Baetidae Callibaetis Odonata Calopterygidae Odonata Calopterygidae Calopteryx Decapoda Cambaridae Mesogastropoda Viviparidae Campeloma Diptera CecidomyiidaeAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata Order Family Genus Coleoptera Dytiscidae Celina Ephemeroptera Baetidae Centroptilum Trichoptera Leptoceridae Ceraclea Diptera Ceratopogonidae Trichoptera Polycentropodidae Cernotina Megaloptera Corydalidae Chauliodes Trichoptera Hydropsychidae Cheumatopsyche Trichoptera Philopotamidae Chimarra Diptera Chironomidae Chironomus Ephemeroptera Leptophlebiidae Choroterpes Coleoptera Chrysomelidae Diptera Chironomidae Cladotanytarsus Diptera Chironomidae Cladotanytarsus Diptera Chironomidae Cladotanytarsus Diptera Chironomidae Cladotanytarsus Diptera Chironomidae Cladotanytarsus A cariformes Clathrosperchonidae Clathrosperchon Megaloptera Sisyridae Climacia Diptera Chironomidae Clinotanypus Odonata Coenagrionidae Veneroida Corbiculidae Corbicula Megaloptera Corydalidae Megaloptera Corydalidae Corydalus Diptera Chironomidae Corynoneura A mphipoda Crangonyctidae Crangonyx Diptera Chironomidae Cricotopus Diptera Chironomidae Cricotopus Diptera Chironomidae Cricotopus or orthocladius Diptera Chironomidae Cryptochironomus Diptera Chironomidae Cryptotendipes Coleoptera Curculionidae Trichoptera Polycentropodidae Cyrnellus Diptera Ceratopogonidae Dasyhelea Haplotaxida Naididae Dero Haplotaxida Naididae Dero Haplotaxida Naididae Dero Haplotaxida Naididae Dero Haplotaxida Naididae Dero Coleoptera Dytiscidae Desmopachria Rhynchobdellida Glossiphoniidae Desserobdella Diptera Chironomidae Dicrotendipes Diptera Chironomidae Dicrotendipes Diptera Chironomidae Dicrotendipes Diptera Chironomidae Dicrotendipes Diptera Chironomidae Dicrotendipes Coleoptera Gyrinidae Dineutus Trichoptera Hydropsychidae Diplectrona Diptera Chironomidae Djalmabatista Coleoptera Dryopidae Coleoptera Elmidae DubiraphiaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche Order Family Genus Coleoptera Dytiscidae Lumbriculida Lumbriculidae Eclipidrilus Lumbriculida Lumbriculidae Eclipidrilus Coleoptera Psephenidae Ectopria Coleoptera Psephenidae Ectopria Diptera Chironomidae Einfeldia Mesogastropoda Pleuroceridae Elimia Coleoptera Elmidae Odonata Coenagrionidae Enallagma Odonata Coenagrionidae Enallagma Haplotaxida Enchytraeidae Diptera Chironomidae Endotribelos Coleoptera Hydrophilidae Enochrus Odonata Libellulidae Epicordulia Ephemeroptera Ephemerellidae Eurylophella Basommatophora A ncylidae Ferrissia

A cariformes Krendowskiidae Geayia Heteroptera Gerridae Rhynchobdellida Glossiphoniidae Diptera Chironomidae Goeldichironomus Diptera Chironomidae Goeldichironomus Diptera Chironomidae Goeldichironomus Odonata Gomphidae Odonata Gomphidae Gomphus Coleoptera Elmidae Gonielmis Coleoptera Gyrinidae Coleoptera Gyrinidae Gyrinus Haplotaxida Naididae Haemonais Odonata Gomphidae Hagenius Basommatophora Physidae Haitia Diptera Chironomidae Hayesomyia Coleoptera Dryopidae Helichus Coleoptera Dryopidae Helichus Rhynchobdellida Glossiphoniidae Helobdella Rhynchobdellida Glossiphoniidae Helobdella Diptera Empididae Hemerodromia Ephemeroptera Heptageniidae Odonata Calopterygidae Hetaerina Odonata Calopterygidae Hetaerina Ephemeroptera Ephemeridae Hexagenia Diptera Tipulidae Hexatoma

A mphipoda Hyalellidae Hyalella Coleoptera Hydraenidae Hydraena Mesogastropoda Hydrobiidae A cariformes Hydrodromidae Hydrodroma Heteroptera Hydrometridae Hydrometra Coleoptera Hydrophilidae Trichoptera Hydropsychidae HydropsycheAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra Order Family Genus Trichoptera Hydropsychidae Hydropsyche Trichoptera Hydropsychidae Hydropsyche Trichoptera Hydroptilidae Hydroptila Coleoptera Dytiscidae Hydrovatus A cariformes Hygrobatidae Hygrobates Odonata Coenagrionidae Ischnura Ephemeroptera Oligoneuriidae Isonychia Diptera Chironomidae Kiefferulus A cariformes Unionicolidae Koenikea A cariformes Krendowskiidae Krendowskia Diptera Chironomidae Labrundinia Diptera Chironomidae Labrundinia Diptera Chironomidae Labrundinia Diptera Chironomidae Labrundinia Diptera Chironomidae Labrundinia Coleoptera Dytiscidae Laccophilus Basommatophora A ncylidae Laevapex Diptera Chironomidae Larsia Diptera Chironomidae Larsia Diptera Chironomidae Lauterborniella A cariformes Lebertiidae Lebertia Lepidoptera Trichoptera Lepidostomatidae Lepidostoma Ephemeroptera Leptophlebiidae Plecoptera Leuctridae Leuctra Odonata Libellulidae A cariformes Limnesiidae Limnesia Haplotaxida Tubificidae Limnodrilus Coleoptera Dytiscidae Lioporeus Isopoda A sellidae Lirceus Diptera Chironomidae Lopescladius Lumbriculida Lumbriculidae Lumbriculus Basommatophora Lymnaeidae Trichoptera Psychomyiidae Lype Ephemeroptera Heptageniidae Maccaffertium Ephemeroptera Heptageniidae Maccaffertium Ephemeroptera Heptageniidae Maccaffertium Odonata Libellulidae Macromia Odonata Libellulidae Macromia Coleoptera Elmidae Macronychus Trichoptera Hydropsychidae Macrostemum Trichoptera Hydroptilidae Mayatrichia Diptera Tipulidae Megistocera Coleoptera Noteridae Mesonoterus Heteroptera Mesoveliidae Mesovelia Trichoptera Brachycentridae Micrasema Coleoptera Elmidae Microcylloepus Basommatophora Planorbidae Micromenetus Basommatophora Planorbidae Micromenetus Diptera Chironomidae MicropsectraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex Order Family Genus Diptera Chironomidae Microtendipes Diptera Chironomidae Microtendipes Diptera Chironomidae Microtendipes Heteroptera Veliidae Microvelia A cariformes Mideopsidae Mideopsis A cariformes Momoniidae Momonia Diptera Chironomidae Monopelopia A rhynchobdellida Erpobdellidae Mooreobdella Haplotaxida Naididae Haplotaxida Naididae Nais(animal) Haplotaxida Naididae Nais(animal) Diptera Chironomidae Nanocladius Trichoptera Leptoceridae Nectopsyche Trichoptera Leptoceridae Nectopsyche Trichoptera Leptoceridae Nectopsyche Ephemeroptera Neoephemeridae Neoephemera Plecoptera Perlidae Neoperla Diptera Empididae Neoplasta Heteroptera Pleidae Neoplea Coleoptera Dytiscidae Neoporus Trichoptera Hydroptilidae Neotrichia Trichoptera Polycentropodidae Neureclipsis Trichoptera Polycentropodidae Neureclipsis Odonata Libellulidae Neurocordulia Odonata Libellulidae Neurocordulia Megaloptera Corydalidae Nigronia Megaloptera Corydalidae Nigronia Diptera Chironomidae Nilotanypus Diptera Chironomidae Nilotanypus Diptera Chironomidae Nilothauma Coleoptera Noteridae Notomicrus Trichoptera Polycentropodidae Nyctiophylax Trichoptera Leptoceridae Oecetis Trichoptera Leptoceridae Oecetis Trichoptera Leptoceridae Oecetis Trichoptera Leptoceridae Oecetis Trichoptera Leptoceridae Oecetis Coleoptera Scirtidae Ora/scirtes Oribatida Diptera Tipulidae Ormosia Diptera Chironomidae Orthocladius Trichoptera Hydroptilidae Orthotrichia A cariformes Oxidae Oxus Trichoptera Hydroptilidae Oxyethira Odonata Libellulidae Pachydiplax Decapoda Palaemonidae Palaemonetes Diptera Ceratopogonidae Palpomyia/bezzia grp. Diptera Chironomidae Parachaetocladius Diptera Chironomidae Parachironomus Diptera Chironomidae ParachironomusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus Order Family Genus Diptera Chironomidae Parachironomus Diptera Chironomidae Paracladopelma Coleoptera Hydrophilidae Paracymus Diptera Chironomidae Parakiefferiella Diptera Chironomidae Parakiefferiella Diptera Chironomidae Paralauterborniella Ephemeroptera Leptophlebiidae Paraleptophlebia Diptera Chironomidae Paramerina Diptera Chironomidae Parametriocnemus Diptera Chironomidae Paratanytarsus Diptera Chironomidae Paratanytarsus Diptera Chironomidae Paratanytarsus Diptera Chironomidae Paratendipes Coleoptera Dryopidae Pelonomus Coleoptera Haliplidae Peltodytes Coleoptera Haliplidae Peltodytes Diptera Chironomidae Pentaneura Plecoptera Perlidae Perlesta Plecoptera Perlidae Perlinella Diptera Chironomidae Phaenopsectra Trichoptera Dipseudopsidae Phylocentropus Basommatophora Physidae Physa Basommatophora Physidae Diptera Tipulidae Pilaria Rhynchobdellida Glossiphoniidae Placobdella Basommatophora Planorbidae Planorbella Basommatophora Planorbidae

Ephemeroptera Baetidae Plauditus Ephemeroptera Baetidae Plauditus Plecoptera Trichoptera Polycentropodidae Trichoptera Polycentropodidae Polycentropus Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Polypedilum Diptera Chironomidae Potthastia Diptera Chironomidae Potthastia Haplotaxida Naididae Pristina Decapoda Cambaridae Procambarus Diptera Chironomidae Procladius Ephemeroptera Baetidae Procloeon Odonata Gomphidae Progomphus Odonata Gomphidae ProgomphusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Order Family Genus Coleoptera Elmidae Promoresia Hoplonemertea Tetrastemmatidae Prostoma Diptera Chironomidae Psectrocladius Diptera Chironomidae Pseudochironomus Ephemeroptera Baetidae Pseudocloeon Ephemeroptera Baetidae Pseudocloeon Ephemeroptera Baetidae Pseudocloeon Ephemeroptera Baetidae Pseudocloeon Diptera Tipulidae Pseudolimnophila Diptera Psychodidae Plecoptera Pteronarcidae Pteronarcys Lepidoptera Pyralidae Diptera Chironomidae Rheocricotopus Diptera Chironomidae Rheotanytarsus Diptera Chironomidae Rheotanytarsus Diptera Chironomidae Rheotanytarsus A cariformes Limnocharidae Rhyncholimnochares Diptera Chironomidae Robackia Ephemeroptera Ephemerellidae Teloganopsis Megaloptera Sialidae Sialis Diptera Simuliidae Simulium Diptera Simuliidae Simulium Megaloptera Sisyridae Sisyra Haplotaxida Naididae Slavina A cariformes Sperchonidae Sperchon A cariformes Sperchonidae Sperchonopsis Coleoptera Hydrophilidae Sperchopsis Coleoptera Hydrophilidae Sperchopsis Veneroida Sphaeriidae(mollusca) Haplotaxida Tubificidae Spirosperma Coleoptera Staphylinidae Diptera Chironomidae Stelechomyia Diptera Chironomidae Stempellina Diptera Chironomidae Stempellinella Diptera Chironomidae Stempellinella Diptera Chironomidae Stempellinella Ephemeroptera Heptageniidae Stenacron Ephemeroptera Heptageniidae Stenacron Coleoptera Elmidae Stenelmis Diptera Chironomidae Stenochironomus Coleoptera Staphylinidae Stenus Haplotaxida Naididae Stephensoniana Diptera Stratiomyidae Odonata Libellulidae Sympetrum Diptera Tabanidae Plecoptera Taeniopterygidae Taeniopteryx Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae TanytarsusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler Order Family Genus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Tanytarsus Diptera Chironomidae Thienemanniella Diptera Chironomidae Thienemanniella Diptera Chironomidae Thienemanniella Diptera Chironomidae Thienemanniella Diptera Chironomidae Thienemannimyia grp. Diptera Tipulidae Tipula Diptera Tipulidae Tipula Diptera Tipulidae A cariformes Torrenticolidae Torrenticola Trichoptera Leptoceridae Triaenodes Trichoptera Leptoceridae Triaenodes Trichoptera Leptoceridae Triaenodes Diptera Chironomidae Tribelos Diptera Chironomidae Tribelos Diptera Chironomidae Tribelos Ephemeroptera Leptohyphidae Tricorythodes Haplotaxida Tubificidae Diptera Chironomidae Tvetenia Diptera Chironomidae Tvetenia Stylommatophora Pupillidae Vertigo Mesogastropoda Viviparidae Viviparus Diptera Chironomidae Xenochironomus Diptera Chironomidae Xestochironomus Diptera Chironomidae Xylotopus Diptera Chironomidae Zavreliella Diptera Chironomidae ZavrelimyiaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis A croneuria lycorias A gnetina annulipes A llonais inaequalis A mnicola A mphipoda A ncylidae A ncyronyx variegatus A nisocentropus pyraloides A pedilum A rgia A rgia fumipennis A rgia tibialis A rrenurus A

sioplax A therix A tractides A trichopogon A ulodrilus pigueti Baetidae Baetis intercalaris Baetisca becki Baetisca rogersi Beardius Berosus Bivalvia Boyeria vinosa Brachycentrus chelatus Brachycentrus numerosus Branchiobdellidae Bratislavia unidentata Brillia Caecidotea Caenis Caenis diminuta Caenis eglinensis Caenis hilaris Callibaetis Callibaetis floridanus Calopterygidae Calopteryx Cambaridae Campeloma Cecidomyiidae Current NameAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Celina Centroptilum triangulifer Ceraclea Ceratopogonidae Cernotina Chauliodes Cheumatopsyche Chimarra Chironomus Choroterpes basalis Chrysomelidae Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. d epler Cladotanytarsus sp. f epler Cladotanytarsus viridiventris Clathrosperchon Climacia areolaris Clinotanypus Coenagrionidae Corbicula fluminea Corydalidae Corydalus cornutus Corynoneura Crangonyx Cricotopus Cricotopus bicinctus Cricotopus or orthocladius Cryptochironomus Cryptotendipes Curculionidae Cyrnellus fraternus Dasyhelea Dero Dero digitata complex Dero flabelliger Dero furcata Dero pectinata Desmopachria Desserobdella phalera Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Diplectrona Djalmabatista pulcher Dryopidae Dubiraphia vittata Current NameAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Dytiscidae Eclipidrilus Eclipidrilus palustris Ectopria Ectopria thoracica Einfeldia sp. a epler Elimia Elmidae Enallagma Enallagma coecum Enchytraeidae Endotribelos hesperium Enochrus Epicordulia princeps regina Eurylophella doris Ferrissia Gastropoda Geayia Gerridae Glossiphoniidae Goeldichironomus Goeldichironomus cf. natans Goeldichironomus holoprasinus Gomphidae Gomphus Gonielmis dietrichi Gyrinidae Gyrinus Haemonais waldvogeli Hagenius brevistylus Haitia Hayesomyia senata Helichus Helichus lithophilus Helobdella stagnalis Helobdella triserialis Hemerodromia Heptageniidae Hetaerina Hetaerina titia Hexagenia Hexatoma Hirudinea Hyalella azteca Hydraena Hydrobiidae Hydrodroma Hydrometra Hydrophilidae Hydropsyche Current Name

PhysaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Hydropsyche incommoda Hydropsyche mississippiensis Hydroptila Hydrovatus Hygrobates Ischnura Isonychia Kiefferulus Koenikea Krendowskia Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia pilosella Labrundinia sp. 3 nr. virescens Laccophilus Laevapex Larsia Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Lepidostoma Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Lioporeus Lirceus Lopescladius Lumbriculus variegatus Lymnaeidae Lype diversa Maccaffertium Maccaffertium exiguum Maccaffertium smithae Macromia Macromia illinoiensis georgina Macronychus glabratus Macrostemum carolina Mayatrichia ayama Megistocera Mesonoterus Mesovelia Micrasema Microcylloepus pusillus Micromenetus Micromenetus dilatatus avus Micropsectra Current NameAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Microtendipes Microtendipes pedellus grp. Microtendipes rydalensis grp. Microvelia Mideopsis Momonia Monopelopia Mooreobdella Naididae Nais communis complex Nais(animal) Nanocladius Nectopsyche Nectopsyche exquisita Nectopsyche pavida Neoephemera Neoperla Neoplasta Neoplea Neoporus Neotrichia Neureclipsis Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Nigronia Nigronia serricornis Nilotanypus americanus Nilotanypus fimbriatus Nilothauma Notomicrus nanulus Nyctiophylax Oecetis Oecetis avara Oecetis cinerascens Oecetis georgia Oecetis sphyra/morsei Ora/scirtes Oribatida Ormosia Orthocladius annectens Orthotrichia Oxus Oxyethira Pachydiplax longipennis Palaemonetes Palpomyia/bezzia grp. Parachaetocladius abnobaeus Parachironomus Parachironomus chaetoalus complex Current NameAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Parachironomus supparilis Paracladopelma Paracymus Parakiefferiella sp. b epler Parakiefferiella sp. f epler Paralauterborniella nigrohalterale Paraleptophlebia volitans Paramerina Parametriocnemus Paratanytarsus Paratanytarsus dissimilis Paratanytarsus quadratus Paratendipes subaequalis Pelonomus obscurus Peltodytes Peltodytes dietrichi Pentaneura inconspicua Perlesta placida complex Perlinella drymo Phaenopsectra flavipes Phylocentropus Physa Physidae Pilaria Placobdella Planorbella Planorbidae Platyhelminthes Plauditus Plauditus bimaculatus Plecoptera Polycentropodidae Polycentropus Polypedilum aviceps Polypedilum fallax Polypedilum flavum Polypedilum halterale grp. Polypedilum illinoense grp. Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum trigonus Polypedilum tritum Potthastia Potthastia longimana Pristina Procambarus Procladius Procloeon Progomphus Progomphus obscurus Current Name Parachironomus longistilusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Promoresia tardella Prostoma Psectrocladius Pseudochironomus Pseudocloeon Pseudocloeon ephippiatum Pseudocloeon frondale Pseudocloeon propinquum Pseudolimnophila Psychodidae Pteronarcys dorsata Pyralidae Rheocricotopus robacki Rheotanytarsus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Rhyncholimnochares Robackia clavige r Serratella deficiens Sialis Simulium Simulium jonesi Sisyra Slavina appendiculata Sperchon Sperchonopsis Sperchopsis Sperchopsis tessellatus Sphaeriidae(mollusca) Spirosperma Staphylinidae Stelechomyia perpulchra Stempellina Stempellinella Stempellinella fimbriata Stempellinella leptocelloides Stenacron Stenacron interpunctatum Stenelmis Stenochironomus Stenus Stephensoniana trivandrana Stratiomyidae Sympetrum Tabanidae Taeniopteryx Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Current Name

Teloganopsis deficiensAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenthID -> Tanytarsus sp. e epler Tanytarsus sp. f epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. n epler Tanytarsus sp. o epler Tanytarsus sp. p epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. y epler Thienemanniella Thienemanniella lobapodema Thienemanniella similis Thienemanniella xena Thienemannimyia grp. Tipula Tipula caloptera Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna/helo Tribelos Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Tvetenia Tvetenia vitracies Vertigo Viviparus georgianus Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zavreliella marmorata Zavrelimyia sp. a epler Current NameAs Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) A blabesmyia (karelia) grp. 0000 A blabesmyia hauberi 0010 A blabesmyia mallochi 1002 A blabesmyia rhamphe grp. 0050 A centrella parvula 0000 A cerpenna pygmaea 0090 A croneuria 0000 A croneuria abnormis 0000

Acroneuria arenosa 0000 A croneuria arenosa/evoluta 0000 A croneuria lycorias 0000 A garodes libalis 0000 A lluaudomyia 0000 A mnicola 0000 A mnicola dalli 0000 A mnicola dalli johnsoni 0000 A mphipoda 0000 A ncylidae 0000 A ncyronyx 0000 A ncyronyx variegatus 0200 A nisocentropus pyraloides 0000 A nopheles 0000 A psectrotanypus johnsoni 0000 A rgia 0300 A rgia fumipennis 0000 A rgia moesta 0000 A rgia sedula 0019 A rgia tibialis 0000 A rrenurus 0000 A rrenurus problecornis 0010 A

stacidae 0000 A therix 0000 A tractides 0000 A trichopogon 0000 A ulodrilus pigueti 0000 A ulodrilus pluriseta 0000 Baetidae 0000 Baetis 6000 Baetis intercalaris 0000 Baetisca rogersi 0000 Beardius truncatus 0000 Belostoma 0000 Belostomatidae 0000 Bivalvia 0000 Bourletiella 0000 Boyeria vinosa 0000 Brachycentrus 0000 Brachycentrus chelatus 0000 Brachycentrus numerosus 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted)

Brachycercus maculatus 0003 Caecidotea 0000 Caecidotea racovitzai 0000 Caenis 0000 Caenis diminuta 0000 Caenis hilaris 0 0 41 0 Callibaetis floridanus 0000 Calopteryx 0000 Calopteryx maculata 0000 Cambaridae 0000 Campeloma 0000 Campeloma limum 0000 Cassidinidea ovalis 0000 Cecidomyiidae 0000 Ceratopogonidae 0012

Cernotina 0000 Chaoborus 0010 Chaoborus albatus 0000 Chauliodes 0000 Chelifera 0000 Cheumatopsyche 1025 Chimarra 0400 Chironomus 0000 Choroterpes basalis 0000

Choroterpes hubbelli 0 0 11 0 Cladotanytarsus 0000 Cladotanytarsus cf. daviesi 0000 Cladotanytarsus sp. i epler 0000

Clathrosperchon 0000 Climacea areolaris 0000 Clinotanypus 0101 Coelotanypus 0000 Coelotanypus scapularis 0001 Coenagrionidae 0000 Collembola 0000 Conchapelopia 0500 Corbicula fluminea 0032 Corduliidae 0000 Corydalus 0000

Corydalus cornuta 0002 Corydalus cornutus 0000 Corynoneura 1000 Corynoneura sp. b epler 0000 Corynoneura sp. c epler 0000 Corynoneura taris 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Crangonyx 0000 Cricotopus 0000 Cricotopus bicinctus 0000 Cricotopus bicinctus grp. 0000

Cricotopus or orthocladius 3000

Cryptochironomus 0000 Cryptotendipes 0000 Curculionidae 0000

Cyrnellus fraternus 0000

Demicryptochironomus 0000

Dero digitata 0000 Dero digitata complex 0000 Dero lodeni 0000 Dero pectinata 0000

Dero trifida 0000 Dero vaga 0000 Dicrotendipes 0000 Dicrotendipes modestus 0000 Dicrotendipes neomodestus 0000 Dicrotendipes simpsoni 0000 Dicrotendipes sp. a epler 1000 Dineutus 1040 Dineutus discolor 0000 Dineutus serrulatus 0000 Diplectrona modesta 0000 Dixa 0000 Dixella 0000 Djalmabatista pulcher 0000 Dolichopodidae 0000 Dromogomphus armatus 0000 Dromogomphus spinosus 1000 Dryopidae 0002 Dubiraphia 0000 Dubiraphia quadrinotata 0 0 14 8 Dubiraphia vittata 0100 Dugesia 0000 Dugesia tigrina 0000 Eclipidrilus 0000 Eclipidrilus palustris 0000 Ectopria 0000 Elimia 0000 Elimia floridensis 4000 Empididae 0000 Enallagma 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted)

Enallagma cardenium 0003 Enchytraeidae 0500 Endotribelos hesperium 0000 Enochrus 0000 Ephemerellidae 0000 Ephydridae 0000 Epitheca 0 0 13 0 Epitheca princeps regina 0000 Eurylophella doris 0000 Eurylophella temporalis 0100 Ferrissia 0000 Gammaridae 0000 Gammarus 6000

Gammarus tigrinus 0000 Gastropoda 0000 Geayia 0000 Gerridae 0000 Gloiobdella elongata 0000 Goeldichironomus fluctuans 0001 Gomphidae 0000 Gomphus 0000 Gomphus minutus 0011 Gonielmis 0000 Gonielmis dietrichi 0000 Grandidierella bonnieroides 0000 Gyretes iricolor 0000 Haber speciosus 0000 Hagenius brevistylus 0000 Hebetancylus excentricus 5001 Helobdella stagnalis 0000 Helobdella triserialis 0000 Helopelopia 1000 Hemerodromia 0100 Hemiptera 0000 Heptageniidae 0000 Hetaerina 0000 Hetaerina titia 0001 Heteroplectron americanum 0000 Hexagenia 0000 Hexagenia bilineata 0000 Hexagenia limbata 1000 Hexagenia munda orlando 0000 Hexatoma 0000 Hyalella azteca 3401 Hydra 0000 Hydrachna 0000 Hydraena marginicollis 0000 Hydraenidae 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Hydrobiidae 0000 Hydrodroma 0100 Hydrophilidae 0000 Hydroporus 0000 Hydropsyche 0 2 01 8 Hydropsyche decalda 0000 Hydropsychidae 0000 Hydroptila 1010 Hydroptilidae 0000 Hygrobates 0000 Hygrotus 0000 Ischnura 0000 Ischnura hastata 2000 Isonychia 0100 Isoperla 0000 Isopoda 0000 Isotomurus 0000 Isotomurus tricolor 0000 Kiefferulus 0000 Krendowskia 0000 Labiobaetis 0000 Labrundinia 0000 Labrundinia becki 0000 Labrundinia johannseni 0000 Labrundinia neopilosella 0000 Labrundinia pilosella 1200 Laevapex 0000 Laevapex peninsulae 0000 Larsia berneri 0000 Larsia decolorata 0000 Lauterborniella agrayloides 0000 Lebertia 0000 Lepidoptera 0000 Leptoceridae 0000 Leptophlebia 0000 Leptophlebiidae 0300 Leuctra 0200 Libellulidae 0000 Limnesia 0010 Limnodrilus hoffmeisteri 2000 Limnophila 0000 Limonia 0000 Liodessus 0000

Liodessus affinis 0000 Lioporeus 0000 Lirceus 0000 Littoridinops monroensis 0000 Lumbriculidae 0000 Lumbriculus variegatus 0100As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Lype diversa 0000 Maccaffertium 0000 Maccaffertium smithae 0000 Macromia 0000 Macromia taeniolata 0002 Macromiidae 0000 Macronychus glabratus 0000 Macrostemum carolina 0000 Mayatrichia 0000 Mayatrichia ayama 0000 Meropelopia 0000 Mesosmittia 0000 Metrobates 0001 Micrasema 1000 Micrasema rusticum 0000 Micrasema wataga 0000 Microcylloepus 0000 Microcylloepus pusillus 0 1 2 1 1 9 Micromenetus 0000 Micromenetus dilatatus 0000 Microtendipes pedellus 2100 Microtendipes pedellus grp. 0000 Microtendipes rydalensis 0000 Microtendipes rydalensis grp. 0000 Mideopsis 0000 Molanna 0000 Mooreobdella 0000 Mooreobdella tetragon 0000 Muscidae 0000 Naididae 0000 Nais behningi 0000

Nais communis 1 80 0 0 Nais communis complex 0000

Nais pardalis 0000 Nais simplex 0000

Nais variabilis 0000 Nanocladius 0000 Nectopsyche 0000 Nectopsyche candida 0000 Nectopsyche exquisita 0041 Nectopsyche pavida 0 0 12 0 Nemertea 0000 Neoperla 0000 Neoperla clymene 0000 Neoporus 0000 Neotrichia 0000 Neumania 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Neumania distincta 0010

Neureclipsis 0000

Neureclipsis crepuscularis 0000 Neurocordulia 0000 Neurocordulia alabamensis 0001 Neurocordulia virginiensis 0000 Nigronia 0000 Nigronia fasciatus 0000 Nigronia serricornis 0000 Nilotanypus 0000 Nilothauma 0000 Notogillia wetherbyi 0000

Nyctiophylax 0000 Odonata 0000 Odontomyia 0000 Oecetis 0001 Oecetis cinerascens 1 1 00 0 Oecetis georgia 0000 Oecetis inconspicua cmplx. 0000

Oecetis morsei or sphyra 0000 Oecetis parva 0000 Oecetis persimilis 0000 Oecetis sp. a floyd 0000 Oecetis sp. e floyd 0000 Ora/scirtes 0000 Oribatei 0000 Orthocladiinae 0000

Orthocladiinae gen. c epler 0000

Orthocladiinae gen. f epler 0100 Orthocladius annectens 0000 Orthotrichia 0000 Oxyethira 1030 Pachydiplax longipennis 0000 Pagastiella 0000 Palaemonetes 0000 Palaemonetes kadiakensis 0000 Palaemonetes paludosus 0005

Palpomyia/bezzia grp. 0300 Paracladopelma 0000 Paracladopelma loganae 0000 Paracladopelma undine 0000 Paragnetina 0000 Paragnetina fumosa 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Parakiefferiella 0000

Paralauterborniella nigrohalterale 0000 Paraleptophlebia volitans 0000

Parametriocnemus 0100

Parametriocnemus sp. f epler 0000

Paranyctiophylax 0000 Paraplea 0000 Paraponyx 0000 Paratanytarsus sp. a epler 2000 Paratanytarsus sp. c epler 0000 Paratendipes subaequalis 0000 Pelecypoda 0000 Pelonomus obscurus 0000 Peltodytes 0000 Pentaneura inconspicua 0000 Perlesta 0000 Perlesta placida 0000 Perlesta placida complex 0000 Perlinella 0000 Petrophila 0000 Phaenopsectra obediens grp. 0100 Phaenopsectra punctipes grp. 1000 Phylocentropus 0400 Physella 0000 Physella cubensis 0001 Piona 0000

Pisidiidae 1000 Pisidium 0060 Planariidae 0000 Planorbella duryi 0010 Planorbella scalaris 0000 Planorbidae 0000 Platyhelminthes 0000 Plecoptera 0000 Plumulariidae 0000

Polycentropodidae 0000

Polycentropus 0005 Polypedilum 0000 Polypedilum aviceps 0000

Polypedilum convictum grp. 0 0 33 2 Polypedilum fallax 0000 Polypedilum flavum 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Polypedilum halterale grp. 0003

Polypedilum illinoense 2 42 0 0 Polypedilum illinoense grp. 0000

Polypedilum scalaenum 0000 Polypedilum scalaenum grp. 0000 Polypedilum sp. a epler 0000 Polypedilum tritum 0100 Pomacea paludosa 4000 Pristina aequiseta 0000 Pristina synclites 0000 Pristinella jenkinae 0000 Probezzia 0000 Procambarus 2010 Procladius 0000
Procladius (holotanypus) 0000 Procloeon viridocularis 0040 Progomphus 0000 Progomphus obscurus 0000 Promoresia tardella 0000

Prostoma rubrum 0000 Psammoryctides convolutus 0000

Pseudochironomus 0000 Pseudocloeon 0000 Psychodidae 0000 Pycnopsyche 0000 Pyralidae 0010 Pyrgophorus platyrachis 0000 Ranatra kirkaldyi 0000 Rhagovelia 0000 Rheocricotopus 0000 Rheocricotopus robacki 3000 Rheocricotopus tuberculatus 0000 Rheotanytarsus 0001

Rheotanytarsus distinctissimus grp. 1 80 0 0

Rheotanytarsus exiguus 0000 Rheotanytarsus exiguus grp. 0800 Rheotanytarsus pellucidus 0000 Scirtes 1000 Scirtidae 0000

Serratella deficiens 0000 Sialis 0100 Silvius 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Simuliidae 1000 Simulium 0410 Slavina appendiculata 0000 Somatogyrus walkerianus 0000 Sperchon 0000 Sperchonopsis 0000 Sperchopsis 0000

Sphaeriidae 0000
Sphaeriidae(mollusca) 0000 Sphaeroma 0000 Sphaeromias 0000 Spirosperma 0000 Spirosperma ferox 0000 Stelechomyia perpulchra 0000 Stempellina 0000 Stempellina sp. a epler 0000 Stempellinella 1000 Stenacron 0000 Stenacron floridense 1000 Stenacron interpunctatum 0000 Stenelmis 3000 Stenelmis decorata 0000 Stenelmis hungerfordi 0080 Stenochironomus 0100 Stenonema 0000

Stenonema exiguum 0 0 01 0

Stenonema smithae 0900 Stratiomyidae 0000 Stylaria lacustris 0000 Synorthocladius 0000 Tabanus 0000 Taeniopteryx lita 0000 Tanytarsus 0000 Tanytarsus sp. a epler 0040 Tanytarsus sp. c epler 7081 Tanytarsus sp. d epler 0000 Tanytarsus sp. e epler 0010 Tanytarsus sp. g epler 0000 Tanytarsus sp. l epler 2300 Tanytarsus sp. m epler 0000 Tanytarsus sp. o epler 0000 Tanytarsus sp. r epler 0000 Tanytarsus sp. s epler 1000 Tanytarsus sp. t epler 0010 Tanytarsus sp. u epler 0000 Taphromysis bowmani 0000As Calculated / STORET station -> 22030062 19010042 28020221 25020111 Sample date -> 3/21/1994 11/1/1994 7/27/1994 10/24/1994

Taxon Name / SampleID/BenID -> SampleID 3105/BenthID 3980(#counted) SampleID 3682/BenthID 4628(#counted) SampleID 3797/BenthID 4802(#counted) SampleID 3952/BenthID 5024(#counted) Thienemanniella 2410 Thienemanniella lobapodema 0000 Thienemanniella similis 0000

Thienemanniella sp. a epler 0000

Thienemannimyia grp. 0000 Tipula 0000 Tipulidae 0000 Torrenticola 0010 Triaenodes 0110 Triaenodes ignitus 0000 Triaenodes perna 0000 Tribelos 0100 Tribelos atrum 0000 Tribelos fuscicornis 0000 Tribelos jucundum 0000 Tricorythodes albilineatus 0000 Tubificidae 0000 Turbellaria 0000 Tvetenia 0000 Tvetenia discoloripes grp. 0000 Unionicola 0000 Unniella multivirga 0000 Veliidae 0000 Xenochironomus xenolabis 0001 Xestochironomus 0000 Xylotopus pa r 0000 Zygoptera 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000 0000 0000 0000 0000 0040 0020 0500

0000 0000 0000 0000 1000 2004 0000 0000 0000 0000 0000 0010 0150 0000 0000 0010 3000 0000 0000 0000 0000 0000 1000 0000 0010 0000 0001 0000 0004 0000 0000 0000 0000 0000 0000 0000 0000 0210 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted)

0000 0000 0000 0000 0000 0000 0000 0010 0000 0110 0000 0000 2000 0000 0000

0000 0000 0000 0000 0000 0 1 40 1 0010 0000 0000

0000 0000 0000 0000

0000 0000 2000 0000 0000 0000 0000 0310 0001 0000 0000

0000 0000 0000 0010 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000 0000 1000 0000

0000

0001 0000 0000

1000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 0200 0000 0000 0000 0000 0000 0000 0300 0000 0000 0000 0000 0033 0000 0000 0000 1 00 0 0 0001 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted)

7000 0201 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

1000 0000 3000 0000 0000 0000 0000 1010 0000 0000 0020 2000 0020 0010 0010 0000 0000 0000 0000 0210 0001 0000 0000 1010 0000 0000 0000 0000 2000 0010 14 0 2 0 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000 0100 0000 0000 1480 0000 0000 0000 0000 0000 0000 0001 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 1000 0000 0000 0000 0040 0080 0000 0000 1 61 1 4 0000 0000 0000

0000 0000 0000 0000 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000 0000 0000 0000 0000 0000 0000 0020 0000 0000 0100 0001 0000 0000 0000 0000 0000 0010 0000 0000 0000 0100 0000 0010 0000 0000 0000 0000 0000 0000 0000

0010 0000

0000 0000

0000 0100 0000 0000 0000 0000 0000 0020 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000

0000

0010 0030 0000 0000 0000 0000 0000 0000 0001 0000

0000 0000 0000 0210 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0100 0000 2000 0000 1010 0000 0000 4000

4013 0000 0000 0000 0010 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 1000

0000 0000

0064

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0800 0000 0000 0000 0000 0000 0000 0001 0000 0000

0 0 1 18 0000 0000 0001 1000 0000 0000 0000 0000

0000

0000 0000 0 12 2 0

0101 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 2010

0100 3 0 16 0

0000 1 00 0 6 0000 0000 0000 0000 0000 0000 0000 0000 0001

0000 0000 0000 0000 0000

0001 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0121 0000 1000

0600

0000 0 2 14 0 0000 0001 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 0000 1210 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0005 0100 0100 0000 0010 0000 0000 0000 0 1 14 1 0000 0000 0054 0000

0005

0010 0000 0000 0000 0001 0000 0000 0000 2000 0000 0000 1000 0000 0010 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20010454 31010050 33010065 20030265 1/31/1995 2/13/1995 7/12/1995 10/2/1995 SampleID 6810/BenthID 7823(#counted) SampleID 7183/BenthID 8268(#counted) SampleID 7224/BenthID 8306(#counted) SampleID 7776/BenthID 8853(#counted) 1001 0000 0000

0000

0000 0000 0001 0000 0000 0000 0000 0000 0000 0014 0000 2000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0200 0010As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0001 0000 2490 0441 0000 4000 0000 0000

0010 0000 0000 0000 0000 0000 0000 12 0 0 0 0000 0304 0000 0020 0000 0000 0000 1003 0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted)

0000 0010 0000 1002 0000 0000 0000 1000 0000 0010 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 3800 0000 0000 0000

0000 0000 0000 0000

0000 0000 0001 0000 0000 0000 0000 2060 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000 0000 0000 0000

0003

0000 2000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1200 0000 0000 0000 0000 0002 5000 0010 0000 0 0 01 1 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted)

0000 8210 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0008

0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0014 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 0 0 17 0000 0010 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0001 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0002 0000 0000 0000 0000 0000 0001 0100 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0070 0000 0000 0000 1 62 2 0 0000 0000 0010

0000 0000 0000 0200 0000 0As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000 0000 0000 0000 0000 0000 0000 0200 0000 0000 0020 0100 0000 0000 0000 0000 1000 0000 0200 0000 0000 0000 0000 0000 0020 0000 0000 0000 0000 0000 0000

0000 0000

0000 0000

0000 0002 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 3000 0000

0000 0000 0000 0211 0000 0000 1000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0004 0000 0010

20 1 1 2 0000 0000 1000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000

0000 0000

5020

0000

0000 0000 0000 0000 0000 2000 0030 0000 0000 2100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

17 24 0 3 0000 0000 0002 0000 0000 0000 0000 0000

0001

0000 0000 0000

4300 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 1 01 0 0

0000 4 1 541 8

0000 2930 0000 0000 0000 0000 0000 0000 0000 2000 0000

0000 0000 0000 0000 0000

0000 0000

0001 0000 0000 0000 0001 0000 0000 0000 0000 3100 0000 0000

0100

0000 1010 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000 2 1 70 0 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0011 0000 0000 0000 0310 0000 1000 1420 0020

0000

1000 0000 0000 0000 0000 0000 0000 0000 0001 0006 0000 0000 0001 1060 0000 0000 1020 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0000 0000 0000

0000

0000 0000 0000 0000 0002 0000 0000 0000 0000 0000 0110 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0020 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 0000 0211 0002 0000 0 1 19 2 0000 0000

0000 0000 0000 0000 0000 0040 0000 2700 0000 2000 0000 1300 0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 1000 0000 0000 0000 0000 7000 0000 0000 0000 0000 0000 0000 0005 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted)

0000 0 0 22 1 0000 0121 0000 0000 0000 0000 0000 0100 0000 0000 3000 0000 0000

1000 0000 0001 0000 0000 4331 0000 1000 0000

0000 1001 0000 0000

0000 0000 0000 0000 0000 0000 1300 0000 0030 0000 0000

0000 0000 0002 0000 0000 0030As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 0000 0001 0000

1000

0000 0000 0100

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0110 0000 0021 0000 0000 0000 1000 0200 0037 0000 0000 5000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted)

0000 0004 0000 0000 0000 0000 0000 0000 0000 0400 0000 0000 1 60 2 2

0000 0000 0000 0000 0010 0000 1000 0000 0000 0000 0000 3000 0000 0000 0000 0000 0000 0000 0000 3112 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 13 0 0 10 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 0000 0000 0000 7001 0000 0000 1 80 0 0 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0100 0000 0000 0000 0003 0000 0060 0000 0000 0000 5001 0000 0000 0000 0000 0000 0000 0000 3021 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0007 4820 0014 0000 0000 0000 0000 0000 0000 0000 0001 0000 0100 0000 0000

1000 0000

0000 0000

0000 0000 1010 0000 0000 0000 0000 0000 0000 0000 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 1000 0000 0100 0000

0000 0000 0000 0000 0000 0000 0103 0000

0001

0000 0000 0000 0010 0000 0000 0000 0000 1201

0800 0000 0001 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000

0001 0 1 10 0

0001

0000

0000 0000 0000 0000 0000 0000 0 1 30 0 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0 0 39 28 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0000

1 01 1 2 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0031

0 0 51 5 0100

0000 2486 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000

0000 0000

0000 0000 0100 0000 0000 0000 0001 0000 0000 0004 0000 0000

0004

0000 0056 0000 0000 0001

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 4430 1000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0001 0100 0000 0000 0000 0020 0000 0500 0261 0000 0000 0201 0040

0001

0700 0000 0000 0000 0000 0000 0000 0000 0000 4000 0000 0000 2010 2000 0000 0000 0001 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20010454 20030341 20030265 20030342 3/4/1996 3/5/1996 3/5/1996 3/5/1996 SampleID 9206/BenthID 10191(#counted) SampleID 9395/BenthID 10288(#counted) SampleID 9396/BenthID 10289(#counted) SampleID 9399/BenthID 10292(#counted) 0000 0000 0000

0000

0000 0001 0000 0000 0000 0200 0000 0000 0000 0000 0000 1 00 0 0 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000 0000 1 1 00 1 0302 0000 0001 2000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0 2 22 0 0000 0121 0030 0000 1000 0310 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0110 2000 0040 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted)

0000 2600 0000 5002 0000 0000 0000 0000 0000 2010 0100 0000 0000 0000 0000

1100 0000 0000 0000 1000 1 70 0 1 0 0 24 2 0000 0000

0000 0010 0000 0000

0000 0000 0000 0000 0000 2000 0000 3000 0002 0000 0000

0000 1021 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0660 0000 0000 0000

0005

0000 0000 0000

0000

0000

0100 0000 0000 0000

0000 0500 0000 0001 0000 0000 0000 0111 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0609 0000 1000 0240 7000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted)

0000 0 1 12 0 0000 0000 0000 0000 0400 0020 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0100 0000 0000 1000 0000 0000 0000 0000 0010 0000 0000 0000 0000 4260 0000 0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000 0100 0000 0000 6031 0000 0000 0000 0000 0000 0000 0105 0000 1000 0000 0000 0000 0000 0100 0000 1002 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 3006 1020 0000 0000 5 1 03 1 0000 0000 0000

0000 0010 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000 0000 0000 0000 0002 0100 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 6 4 01 9 0 1 10 0 0000 0000 9210 0000 3020 1100 0000 0000 0000 0000 0000 0000

0000 0000

0000 0010

0000 0100 0000 0000 0000 0000 0000 0000 0000 2000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000

0000

0000 0000 0001 0000 2000 0010 0000 0010 0100 0000

0000 0000 0000 5200 0000 0000 0000

0000 0050 0000 0000 0000 0000 0100 0000

0000

0000 2000 0000 0000 0100 0000 0000 0000 0001

1126 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000

0000 0000

0000

0000

0000 0000 0000 0000 0000 0000 0 1 20 0 0000 0000 1000 0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 3000

0043 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 1 40 0 0

0032 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 1010

0700 2000

0000 2000 0000 0000 0000 0000 0000 0100 0000 0000 0100

0000 0000 0000 0000 0000

0000 0000

1000 0000 0000 0000 0000 0000 0000 0000 0000 1010 0000 0020

0001

0000 2000 0000 0000 0000

1000 0300 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000 0053 2200 0010 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0210 0000 0060 0001 1 3 12 11 0000 0000 2171 0000

0003

1070 0000 0000 0000 0000 0000 0000 0000 0007 3010 0000 0000 0010 0030 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 31010050 19010099 32020063 28020221 7/9/1996 7/9/1996 7/16/1996 9/16/1996 SampleID 10131/BenthID 10723(#counted) SampleID 10591/BenthID 11209(#counted) SampleID 10633/BenthID 11325(#counted) SampleID 10785/BenthID 11544(#counted) 0000 0000 0000

2000

0010 0000 0000 0100 4101 0000 0000 0000 0000 1200 2400 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 0000 0003 0011 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0010 0220 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0200 0000 1000 0000 0000 3000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted)

0000 0000 0000 0000 0000 0000 0000 0000 0010 1000 0000 0000 0000 0000 0000

0100 0000 0000 0000 0000 13 28 0 0 0000 0000 0000

0000 0100 0000 0000

0000 0000 0000 0000 0000 0000 0000 0060 2004 1000 0000

0000 0000 0000 0000 0100 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 0000 0000 1500

1 10 0 1

0001 7000 0000

0000

0000

0000 0000 0000 0000

0001 0000 0000 0003 0000 0000 0000 0000 0000 0000 0000 0000 0100 0003 0000 0000 0000 0000 0040 0000 0004 0000 2000 1040 0000 0000 0000 0 1 90 0 0000 0300As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted)

0001 0 0 30 0 0000 0000 0000 0000 0000 0000 0000 0030 0000 0000 6000

0000 0000 0000 0000 0000 0000 0000 2000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0400 0000 0000 0001 0000 0000 0010 0000 0000 0000 0000 11 9 0 27 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 0000 0000 0020 2

50 0 0 0000 0000 2303 0000 0000 0010 0004 0000 0000 0000 0000 0000 0100 0000 0000 1203 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 3100 0001 0000 0000 0070 0010 0000 0000 1 70 0 4 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 0000 0000 0000 0001 0000 0100 0000 0000 0000 0000 0000 0000 0500 0000 0000 0000 0 0 01 0 0000 0000 0 0 16 0 0000 0000 0000 2010 0010 0000 0001 0000 0000 0000

0000 0000

0000 0000

0000 0000 2000 0000 0007 0000 0000 0000 0000 0000 0000 0 0As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0002 0000

0010 0000 0000 2116 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0500 0111 0000 0000 0000 0000 2000

5051 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000

2010 0000

0330

0000

0000 0000 0000 0000 0300 0000 0000 0000 0000 4100 0000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000

0005 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0010 0000

18 1 0 14 0003 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 1000

0000 2 1 10 2

0000 0 0 01 3 0000 0000 0000 0000 0000 0000 0000 0000 0020

0000 0000 0000 0000 0000

0000 0000

0000 0000 0000 0000 2000 0000 0000 0000 0000 0801 0080 0000

1700

0000 2001 0000 0001 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 1020 0100 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0104 0010 0000 0000 0002 0000 0000 0511 0000

0500

0000 0000 0000 0000 0000 0000 0000 0000 0040 0000 0000 2000 1146 0094 0050 0000 0032 0200 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20020317 22030062 33040064 26011019 9/16/1996 2/11/1997 2/25/1997 2/18/1997 SampleID 10844/BenthID 11554(#counted) SampleID 11464/BenthID 11963(#counted) SampleID 11607/BenthID 12057(#counted) SampleID 12089/BenthID 12197(#counted) 0000 0000 3000

0000

0020 0000 0000 0000 0100 0000 0000 0010 0000 0001 0000 4000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0002As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000 0000 0010 0000 0000 0210 1 40 0 1 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0040 1000 0007 0000 0100 0000 1000 0000 0000 0000 0000 0201 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0004 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted)

0000 0000 0000 0000 0000 0000 0010 0040 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0200 3000 0000 0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 1011 0100 0000 0000

0000 0000 0010 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000 0000 0000 0000

0000

0000 0000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0100 0000 0000 0000 0000 0002 0000 0200 0000 0000 1000 0000 0001 0000 0000 0000 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted)

0000 1103 0000 0000 0000 0002 0000 0000 8000 0000 0000 0000 0010

0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 2003 0000 0000 0000 0000 0000 0000 0000 0000 0 5 15 2 0000 0000 0000 0000 0000 0010 0000 0000 0000 1000 0 11 0 0 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000 0000 0000 0000 2009 0000 0000 0400 0000 0000 0000 0010 0000 0000 1 90 0 0 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000 0000 0001 0010 0000 0000 0000 0000 0000 3011 0 0 20 8 0000 0000 0011 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0001 0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0 1 40 9 0000 0000 0000 0020 0001 0000 0000 0000 0000 0000 0000 0000 0000

0210 0000

0100 0000

0000 0000 0010 0000 0100 0000 0000 0000 0000 0000 0100 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 1900 0000 0 0 10 0 0000

0001 0000 0000 0000 0000 0000 0100 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0300 0000 0000 0000 0000 0001As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000

0000 0000

0041

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 2

10 0 0 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000

0 1 10 0 0000 0000 0000 0000 0000 0000 0000 0000

0000

0010 0000 0000

2 0 01 6 1001 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000

0000 0402

0000 0021 0000 0000 0000 0000 0000 0000 0000 2020 0000

0000 0000 0000 0000 0000

0200 0000

0002 0000 0000 1000 0000 0000 0000 0000 0000 2210 0000 0000

0920

0 0 0 12 6 5 11 0 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0002 6040 0000 0000 0010 0001 0000

0000

0000 0000 0000 0000 0000 0001 0001 0000 1000 0000 0000 0000 3350 0 0 01 9 0000 9010 0001

0100

1 30 6 0 0000 0100 0000 0000 0000 0000 0000 1000 0002 0000 0000 1040 0400 0001 0000 0010 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 31020040 26011020 20030456 33010054 2/4/1997 2/18/1997 7/2/1997 8/12/1997 SampleID 12092/BenthID 12201(#counted) SampleID 12224/BenthID 12340(#counted) SampleID 12401/BenthID 12463(#counted) SampleID 12436/BenthID 12486(#counted) 0000 0000 0000

0010

0011 0000 0000 0000 0000 0000 0000 0000 0000 0000 1210 0000 0000 0000 0000 2000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000 0000 2201 0210 0000 0400 0 0 01 8 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0100 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted)

0000 0000 0000 0101 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

1000 0000 0000 0000 0000 3

30 0 0 5100 0100 0000

0000 0000 0000 0000

0010 0000 0000 0000 0000 0000 0000 0020 0000 0000 0001

0000 1000 0000 0030 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 4000 0000 0000 0000

0000

0010 0000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0010 0001 0000 0000 0000 0000 0100 0000 0000 0000 0000 0210 0000 0000 0000 0000 0014 0400 0000 0000 0000 0000 0400As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted)

0000 0 7 32 3 0000 0000 0000 0000 0000 0000 0000 0000 0200 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0040 0000 0000 0000 0000 0000 0000 0000 0000 5412 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0500 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000 0000 0100 0000 4045 0000 0000 0000 0000 0000 2000 0000 0000 0000 0002 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000 1200 0000 0000 0000 0000 0000 0000 0000 0000 0000 0220 0010 0000 0000 0100 0000 0300 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 6 1 01 0 0000 0000 0000 0021 0000 0001 0000 0000 0000 0000 0000 0000 0000

0001 0000

0000 0000

0000 0000 4010 0000 0000 0000 0000 0000 0000 0000 2500 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0500 0000 0000 0000

0000 0000 0000 0000 0000 0000 1001 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0100 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000

0000 0000

2000

0 0 14 5

0000 0000 0000 0000 0000 0000 0000 0000 0000 0030 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0600 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0010

1 96 3 1 0100 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0100

0000 0000

0000 5000 0000 0000 0000 0000 0200 0000 0001 0009 0000

0000 0000 0000 0000 0000

0000 0000

0001 0000 0000 0000 0000 0000 0000 0010 0000 2032 0001 0000

3000

0000 4350 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0060 2 5 06 0 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 1045 0000 0000 2822 0010

0000

1003 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0101 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 21010018 26010972 22020062 31020040 7/17/1997 9/30/1997 8/12/1997 2/10/1998 SampleID 12450/BenthID 12500(#counted) SampleID 12600/BenthID 12548(#counted) SampleID 12609/BenthID 12557(#counted) SampleID 13475/BenthID 12867(#counted) 0000 0000 0000

0010

0007 0000 0000 0000 2000 0000 0000 0000 1000 0000 0100 0000 0000 0000 9000 0000 0000 0000 0000 0000 0100 0000 1000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0200 1002 0000 0100 0100 0000 0000 3200 0000 0000 0000 0030 0000 0000 0000 0000 0000 0000 0000 0000 0000 0005 0000 0000 0000 0000 0000 0000 0000 0140 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted)

0000 0000 0000 0000 0000 0000 0000 0000 1000 1000 0000 0000 0000 0000 0200

0010 0000 0000 0000 0000 4207 0 1 1 1 5 0 0000 0000

0000 2000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0010 0 0 01 6 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 1024 0000 0001 0000

1000

0000 0001 0000

0000

0100

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted)

0000 0002 0000 0000 0000 0000 0000 0000 0500 0000 0000 0000 0003

0000 0000 0000 0000 0000 0000 0102 0000 0000 0000 0000 0000 0000 1005 0000 0000 0000 0000 0000 3 0 14 2 0000 0100 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000 0000 0000 0000 0001 0000 0000 0 0 02 0 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0008 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 4010 0 3 97 0 0000 0000 0004 1000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0011 0000 0000 0000 0000 0000 0000 0000 1000 0000 0 3 10 0 0000 0000 0000 0000 1000 0000 1022 0000 0000 0000 1 1 00 0 0000 0000 0000 0000 0000 0000 0000 0100 0000

0002 0000

0000 1000

0000 0000 2000 0000 0001 0000 0000 5030 0000 0000 7001 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000

0400

0000 0000 0000 0000 0000 0000 0200 0000 0100 0000

0000 0000 0000 0000 0000 3040 0000

0000 0000 0000 0400 0000 0000 0000 0610

0000

0000 18 0 0 0 0000 0000 0000 0000 0000 0000 0000

0011 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000

0000 0000

0000

9080

0000 0000 0000 0000 0000 0000 0000 0000 0000 3000 0050 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0001 0000 0000 0000

0000

1000 0000 0010

0010 0300 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 4000

0200 0030

0300 0000 0001 0000 0000 0001 0000 0000 5000 0010 0000

0000 0000 0000 0000 0000

0000 0000

0000 0000 0000 1010 0000 0000 0000 0000 0000 2000 0000 0000

1012

0700 6032 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000 4 0 17 22 0000 0000 0000 0000 0000

0000

0000 0000 0001 0000 0000 0100 0000 0000 1000 0000 0000 0000 4296 0000 0000 3411 0000

0003

0 0 14 0 0000 0000 0000 0000 0000 0000 0000 0000 0500 0000 0000 0001 1600 0500 0000 4000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20030414 33040064 20030456 20020012 3/10/1998 7/1/1998 2/12/1998 8/11/1998 SampleID 13535/BenthID 12898(#counted) SampleID 14213/BenthID 13118(#counted) SampleID 14231/BenthID 13136(#counted) SampleID 14930/BenthID 13379(#counted) 0000 0000 1001

1000

4010 0000 0010 1000 0000 0103 0000 0000 0000 0000 0300 0 0 01 1 0000 0001 0000 0000 0000 0000 0000 0000 0003 0110 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000 0000 0506 0008 0000 0000 1100 0000

0000 0000 0000 0000 0000 0000 0000 0000 0100 0200 0000 1000 0100 0000 0000 1000 0000 0000 0030 0000 0000 0000 0000 1000 0000 2000 0000 0000 0010 0000 0000 0000 0001 0000 0000 0000 0000 1000 0000 0000 0300As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted)

0010 0203 0000 2 0 02 0 0000 0010 0000 0000 0000 2500 0000 0000 0000 1100 0030

0010 0000 0000 0000 0000 3

50 3 0 5000 0000 0000

0000 0000 0000 0000

0000 0000 0200 0000 0000 0000 0000 2200 0020 0 0 01 6 0000

0000 4000 0200 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000 0000 0000 0030

2000

0010 0000 0000

0000

0000

0000 0000 0000 0001

0000 0000 0000 0006 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 0 15 0 0000 0000 0000 0001 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted)

0009 0 1 10 0 0000 0002 0200 0000 0000 0000 0000 0000 0000 0000 0000

0000 0001 0000 0001 0000 0000 0000 0000 0000 0000 1200 0000 0000 0000 0000 0020 0000 0010 0000 4100 0000 0000 0000 0020 0000 0000 1000 0000 0000 2000 0 0 02 5 0000 0000 0000 1000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000 0000 0000 0000 2

00 6 0 0000 0000 4000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 0 01 3 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 2000 0005 1200 2600 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 1000 0000 0000 0000 0000 0000 2

00 0 0 0000 0000 0000 0000 0000 0000 1000 0000 0000 0007 0130 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000

0000 0000

0000 0000

0000 0000 0000 0000 0020 0030 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0010 0000

0000 0000 0000 0030 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0007 0000 0000

1008 0000 0000 0000 2000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000

0000 0000

0000

2000

2100 0000 0000 0000 0000 0000 0001 0000 0000 0410 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0001

0000

0000 0000 1200

1 6 14 0 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000

0000 2204

0000 2010 0000 0100 0000 0000 0001 0000 0000 0000 0000

0000 0000 0000 0000 0000

0000 0000

0100 0000 0000 0000 0000 0080 0000 0000 0000 3000 0000 0000

0000

0000 5080 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000 7000 0000 0000 0000 0000 1000

0000

0000 0000 0000 0000 0000 3100 0000 0000 0000 0000 0000 0000 3 9 11 7 0000 0000 0 1 11 0 0020

2400

2200 0000 0000 0000 2000 0000 0100 0000 0000 1000 0000 0000 1024 2000 1000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 33040014 22020062 25020111 28010223 7/15/1998 9/9/1998 2/17/1999 3/22/1999 SampleID 14965/BenthID 13401(#counted) SampleID 14966/BenthID 13403(#counted) SampleID 15951/BenthID 14187(#counted) SampleID 16636/BenthID 14709(#counted) 0000 0000 0000

1000

2200 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0050 0000 0000 0000 1000 0000 0000 1000 0000 0000 0000 1000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000 0000 0320 0200 0000 0070 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0500 0000 2200 0000 0000 0000 1110 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 0 0 01 6 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted)

0000 2500 0000 0 1 32 3 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0001

0000 0000 0000 0001 0000 0000 1 30 0 0 0000 0 0 01 2

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0 0 15 0 0040 0000

0000 0000 0000 0000 0000 0001As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 3001 0000 0000 0000

0000

0101 0000 0000

0000

0000

0000 0000 0000 0000

0000 0001 0000 0000 0000 0200 0000 0000 0000 0002 0000 0000 0000 0030 0000 0000 0000 0000 0000 0000 0020 0000 0000 0000 2000 0000 0000 0000 0000 0010As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted)

0000 2010 0000 0000 0000 0000 0000 0000 4000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0050 0000 0000 0000 0000 0000 0000 0000 0000 0002 0000 0000 0000 3020 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0 1 32 3 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0001 0000 0000 0000 1000 0000 0120 1030 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0200 0000 0002 0000 0000 0003 0000 0000 0000 0001 0000 0030 0000 0000 0000 0600 0000 0400 0000 1010 0000 0000 0000

0000 0000 0000 0000 0000 0010As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 2 25 1 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0030

0000 0000

0000 0000 0000 0000 0 0 14 0 0010 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000

0000

0000 0000 0000 2000 0000 0000 0000 0000 1000 0000

0000 0001 0000 0000 0000 0000 0050

4000 0000 0000 0000 0000 0000 0000 1000

0000

0000 0000 0000 0000 0000 0000 0000 0001 0000

0020 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000

0000 0000

0000

0000

2000 0000 0000 0000 0000 0000 0000 0001 0000 0000 4000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0 38 0 0 0000 0000 0000

0000

0000 0000 3000

3000 0020 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000

0000 0 0 01 7

0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0100

0000 0000 0000 0000 0000

0000 0000

0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000

0002

0000 1 0 81 0 0000 0010 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000 18 0 1 15 0000 0000 0000 0000 0000

0000

0400 0000 0000 0000 0000 0000 5000 0000 0000 0000 1000 0000 0645 0000 0000 1 1 92 1 0300

3000

5010 0000 0000 0000 1000 0000 0000 0000 0002 0000 0000 0000 0 0 21 3 0000 0000 0000 0000 0001 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 32020063 21010018 26011020 28010223 1/21/1999 7/27/1999 7/20/1999 10/7/1999 SampleID 16643/BenthID 14716(#counted) SampleID 18076/BenthID 15790(#counted) SampleID 18372/BenthID 15965(#counted) SampleID 18594/BenthID 16087(#counted) 0000 0000 0000

0000

3000 0000 0000 0000 0500 0000 0000 0000 0000 0000 0100 0000 0000 0003 0000 0000 0000 0000 0000 0400 0020 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000 0000 1 7 11 0 0010 0000 0000 0000 0000

0000 0000 0000 3000 0000 0000 0000 0000 0000 3000 0000 0100 2000 0000 0000 0103 0000 0000 0000 0000 1000 0300 0000 0000 0001 0001 1000 0000 0000 0100 2000 0000 0020 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted)

0000 0860 0000 0102 0000 0000 0000 2001 0000 0000 0300 0000 0000 0000 0000

0000 0000 0000 0000 0000 8005 8000 0000 0000

0000 1040 0000 0000

0000 0000 0020 0000 0000 2000 0000 2000 0000 0000 0000

0000 0000 0010 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000 0000 0000 0050

0000

0010 0000 0000

0000

0000

0000 0003 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 4000 0000 0000 0000 0000 0000 0000 0111 0000 0000 3000 0003 0000 0000 0000 0000 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted)

0000 0100 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0030

0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 2028 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 15 4 10 0001 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000 0010 0000 0500 1000 0000 0020 0000 0000 0000 0000 0002 0000 0000 0000 0000 0000 0000 0000 0020 0000 0000 0000 0000 0020 0000 0000 0000 0000 0000 0800 0100 0000 0000 0000 8 1 30 0 0000 0100 0000 1011 0000 0000 0000

0000 0000 0000 0000 0000 0010As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 0 01 4 0000 0000 0000 3100 0000 0000 0001 0000 0000 0000 0000 0000 0000

0000 0001

0000 0000

0000 0000 0000 0000 0000 0050 0000 1000 0000 0000 2000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000

0000

0000 0000 0000 0001 0000 0000 0000 0000 1000 0000

0000 0000 0000 0000 0000 1010 0000

0000 0000 0009 0000 0010 0000 1000 0100

0000

0000 0000 0000 0000 0000 0000 0010 0000 0000

0021 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000

0000 0000

4000

0000

0001 0000 0000 0000 0 0 15 0 0000 0400 0000 0000 4000 0000 0000 0000 0000 0000 0000 0000 0010 0300 0000 0000

0000 0000 0002 0000 0000 0800 0000 0000 0000

1000

0001 0000 0000

0 0 03 2 0010 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 1000

0000 0221

0000 0041 0000 0000 0040 0003 0000 0000 0000 0001 0000

0000 0000 0000 0000 0000

0000 0000

0020 0005 0000 1000 0000 0000 0000 0000 0000 0001 0100 0000

1000

0000 6 2 0 10 0000 0001 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0000 2107 0 0 01 0 0000 0000 0000 0000

0000

0000 0000 0030 0000 0000 0000 0000 0000 0000 0200 0002 0000 2210 0000 0000 1714 0110

0006

5000 0000 0000 0000 0000 0000 0000 1 10 0 4 0100 0000 0000 0000 0000 0000 2000 0000 0000 0 0 12 0 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20030414 19010099 21030064 26010972 1/12/2000 2/1/2000 3/15/2000 1/10/2000 SampleID 20471/BenthID 17104(#counted) SampleID 20474/BenthID 17107(#counted) SampleID 20479/BenthID 17112(#counted) SampleID 20480/BenthID 17113(#counted) 0010 0000 0000

0000

0200 0000 0000 0000 5203 0000 0000 0000 0000 0001 0001 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000 0000 0101 0000 0000 0700 0 0 42 0 0000

0000 0000 0000 0000 0000 0000 0000 0000 0010 1100 0000 0000 0000 0000 0000 0620 0000 0000 0000 0000 0000 0000 0000 0000 0000 1500 0000 0000 0000 0000 0000 0004 0000 0000 0000 0000 0000 0010 0000 0010 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted)

0000 0200 0000 0102 0000 0000 0100 0010 0000 0020 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 4 0 02 6 0061 0000 0000

0000 0000 0000 0000

0100 0000 0010 0000 0000 0000 0000 0030 0000 0000 0000

0000 2001 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000 0000 0010 4000

0100

0000 2000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0100 0000 0000 0000 0003 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 2340 0000 0000 0030 1000 0010 0400 2000 0000 0300As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted)

0000 0010 0000 0000 0000 0000 0000 0000 0 0 14 0 0000 0000 0100 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0003 0060 0000 0000 2000 0000 0000 0000 0000 0000 3045 0000 0064 0000 1000 0000 0026 0000 0000 0000 0010 2

87 0 0 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0500 0310 0000 0000 0020 0000 0100 0020 0002 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0100 0000 0000 0000 0000 0000 0000 0000 0003 0100 0000 0000 0010 0000 0000 0000 5011 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0002 0000 0000 0000 0000 0000 0013 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 3010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000

0100 0000

0000 0000

0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 3000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000

0001

0000 0000 0000 0000 0000 0000 0010 0000 0000 0000

0000 0000 0200 1200 0000 0020 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0010 0000 0010 0000 0000 4200 0000 0000

0000 0001 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000

0000 0000

0011

0000

0001 0000 0000 0000 0200 0000 1001 0000 0000 1100 0020 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0 0 1 21

13 3 7 0 0001 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000

0000 0900

0000 2201 0000 0500 0000 0000 0000 0000 0000 0000 0010

0000 0000 0001 0000 0000

0000 0000

0010 5000 0000 0000 0000 0000 0000 0100 0000 0001 0000 0000

0100

0000 18 0 3 5 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000 3 0 51 0 0000 0000 0000 0000 0000

0000

0000 0000 2000 0000 0000 2000 0000 0000 0000 0000 0000 0000 3 2 13 4 0000 0000 0011 0000

2000

0000 0000 0000 0001 0000 0000 0011 0000 0000 0000 0000 0000 1100 1100 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 20020012 22030010 33010054 33040014 2/15/2000 8/22/2000 2/22/2001 2/8/2001 SampleID 21571/BenthID 17864(#counted) SampleID 22114/BenthID 18267(#counted) SampleID 24523/BenthID 19456(#counted) SampleID 24524/BenthID 19457(#counted) 0000 0000 0000

0000

0005 0000 0000 0000 0000 0000 0200 0000 0000 0200 0002 7 1 00 0 0000 0000 0100 0000 0000 0000 0000 0000 6000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000 0000 0013 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 7000 0000 0000 0010 0000 0001 0000 0000 0000 6030 0000 0000 0000 0000 1010 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted)

0000 4 0 01 4 0000 0 0 17 0 0000 0000 0510 0010 0000 0100 0000 0002 0000 0000 0000

0000 0000 0000 0000 0000 0 3 52 0 0001 0000 0040

0000 0010 0000 0000

0100 0000 0000 0001 0000 0000 0000 0000 0000 0000 0000

0000 0010 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0004 1000 0 1 30 0 0000

0000

0000 0000 0000

0000

0000

0000 0000 0010 0001

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0120 0000 0000 0000 0000 0000 0000 9 0 13 5 0000 0000 0002 0000 0000 1 00 0 0 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted)

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0600

0000 0000 0000 0000 0000 0000 0000 0030 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0320 0000 0000 0000 1000 0000 0000 0000 0000 11 10 2 0 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000 6001 0000 0000 0 1 12 0 0000 0000 0300 0000 0000 0000 0002 0000 0000 0000 0000 0000 0000 0000 2020 0000 0001 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0333 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 2250 0030 0007 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000

0000 0000

0000 0000 0030 0000 0000 0000 0000 0000 0000 0000 0000 1000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000

0000

0000 0000 0010 0000 0000 0000 0000 0000 0000 1000

0000 0000 0000 0200 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0100

0000

0000 0000 0000 0030 0000 0000 0000 0000 2000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000

0000 0000

0000

0000

0000 0000 1000 0000 0001 0000 0000 0000 0001 0510 0000 0000 0000 0000 0200 0000 0001 0000 0000 0000 0000

0000 0000 0001 0000 0000 0000 0000 0000 0000

1000

0000 0000 0000

0 22 10 0 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000

1 10 0 0 0005

0000 0000 0000 0000 0000 0000 0000 0000 0000 4000 0000

0000 0000 0000 0000 0000

0000 0100

0500 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0010

0000 0090 0000 1000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000 0 0 02 6 0051 0000 0000 0000 0000

0 0 01 6

0000 0200 0000 0000 0001 0000 2000 0000 0000 0000 0000 2000 0061 0000 0000 0110 0000

0000

0000 0000 0000 0000 0000 0000 2000 0000 0200 0000 0000 0000 0002 0010 0000 0000 0000 0032 0002 1000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera 22030010 20020317 26011019 19010042 9/18/2001 9/4/2001 9/5/2001 7/10/2001 SampleID 25672/BenthID 19985(#counted) SampleID 26863/BenthID 20697(#counted) SampleID 26865/BenthID 20699(#counted) SampleID 26868/BenthID 20702(#counted) 0000 0000 0100

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0010 0003 8400 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0020As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000 0000 0000 0100 0000 0000 0000 0200

0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 1101 0000 0000 0000 4101 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted)

0000 0 0 01 0 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0100

0000 0000 0000 0000 0000 0091 1 61 6 0 0000 0000

0000 0000 0000 0400

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 1020 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0051 0000 0000 0000

0000

0000 0000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 2000 0000 0000 0113 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted)

0000 0000 0000 0000 0000 0000 0000 0000 0721 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0004 0000 0000 0000 2000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000 0000 0000 0000 5101 0000 0000 0000 0000 0000 0000 0003 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 0 01 4 0300 0010 0001 0000 0200 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000 0223 4

10 0 0 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0900 0000 0000 3 0 17 0 0000 0000 0000 0052 0000 0200 0000 0000 0000 0000 0000 0000 0000

0000 0000

0000 0000

0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 2000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0300 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000

0100 0000

0200

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0200 0100 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0400

0000 0000 5 0 29 10As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000

0000 0011

0000 0201 0001 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0600

0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0030 0000 0000 0000

0000

0000 6 4 1 19 0100 0000 0000

0000 0000 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0000 1 1 61 2 0020 0000 0000 0000 0000

0000

0000 0000 0000 0002 0000 0002 0000 0000 0000 0100 0000 0000 1093 0000 0000 8114 0000

0000

0000 0000 0000 0000 0000 0100 0000 0001 2001 0200 0000 0000 0039 0200 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera BAK210GS 32030023 CLA254LR CLA243LV 11/1/2005 2/15/2006 11/14/2005 11/14/2005 SampleID 47698/BenthID 32249(#counted) SampleID 48420/BenthID 33507(#counted) SampleID 50131/BenthID 33604(#counted) SampleID 52996/BenthID 33624(#counted) 0001 0000 0000

0000

6210 0000 0000 0000 0000 0000 0000 0000 0000 0001 0 1 00 1 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000 0000 0000 0000 1000 3000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0200 1000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0000 0000 0000 0000 0000 0030 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 1100 0000 0100 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted)

0000 0800 0000 4010 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 4420 5534 0000 0000

0000 0000 0000 0000

0000 0100 0000 0000 0000 2000 0000 0000 0010 0000 0000

0000 1100 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 2001 0000 0000 0000

0000

0000 0000 0000

0000

0000

0000 0000 0000 0000

0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 4000 0000 0000 0019 0000 0000 0000 0000 0000 0100As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted)

0000 0005 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000 0000 0000 0000 3000 0400 0000 0000 0000 0000 0000 0000 0000 0020 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0300 0000 0000 0000 0000 0000 0000 0000

1000 0000 1 80 0 0 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000 8 1 00 3 0040 0110 0000 0000 1100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1 1 20 0 0000 4000 0000 0500 0000 0000 0000 0000 0000 0000 0000 0000 0010

0000 0300

0000 0000

0000 0000 0000 0000 0000 0000 1000 0000 0040 0000 0010 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000

0000

0000 0500 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0001 0000 0000 0000

0000 0000 0000 0000 0000 1000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0100 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000

0000 0000

0010

0000

0000 1000 0000 0000 0000 0000 0000 0000 0000 0110 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000

0001

0000 0000 0000

0000 0000 11 3 7 3As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000

0000 0040

0000 0 0 11 0 0000 0000 0000 0000 0000 0000 0000 0000 1000

0000 0000 0000 0200 0000

0000 0000

0000 1330 0000 0000 0000 0000 0000 0000 0000 0600 0001 0000

0000

0000 0 4 10 15 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0000 9 7 05 5 0000 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0010 0000 0000 0000 0000 1 9 23 1 0000 0000 1222 0000

0000

0000 0000 0000 0000 0000 0000 0100 0000 0052 0010 0000 0000 1011 2000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera LEV502GS PUT308GS 21010008 BAK210GS 12/6/2005 12/13/2005 4/24/2006 3/20/2006 SampleID 52471/BenthID 34244(#counted) SampleID 52912/BenthID 34645(#counted) SampleID 53274/BenthID 34807(#counted) SampleID 53732/BenthID 35065(#counted) 0010 0000 0000

0000

0154 0001 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 3100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000 0000 1001 0005 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0039 0000 0000 0000 0000 0000 0000 0000 0 0 01 8 0000 0000 0000 0000 0000 0020 0200 0000 0000 0000 0 0 14 0 0000 0000 0000 0000 0600 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted)

0000 0000 1000 0100 3

10 1 0 0000 0000 0011 0000 0000 0000 0000 0000 0000 0000

0100 0000 0000 0000 0000 1 0 15 2 0000 0000 0000

0000 0000 1000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0100 0010 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0002 0000 0000 0000

0000

1000 0000 0000

0000

0000

0000 1700 0000 0000

0000 0000 4000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 1000 0000 0000 0000 0000 7 1 80 4 4000 0000 0000 0102 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted)

0000 0100 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000

0000 0100 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 5000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000 0000 0000 0000 0004 0000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0004 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000 0000 0 0 13 0 0000 0000 0000 0030 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 26 13 1 7 0000 0000 0000 0000 0000 0000 0200 0000 0000 0000 0000 0000 0000

0000 0000

0000 0000

0020 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000

0000

0000 0003 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0008 0000 0000 0000

0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0000 0000 0300 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000

0000 0000

0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0100 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000 0000 0000 0000 0000 0000 0400 0010 0000

0000

0000 0000 0000

0000 0000 0 2 16 0As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000

0000 0001

0000 0000 0000 1 00 0 0 0000 0000 0000 0000 0000 0000 0000

0100 0000 0000 0000 0000

0000 0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000

0000

0000 0 1 91 0 0000 0000 0000

0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000 0 0 23 0 2302 0000 0000 0000 0000

0000

0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0 4 11 0 0000 0000 5100 0000

0000

0000 0000 0000 0000 0000 0000 4010 0300 0300 0000 0000 0000 0002 0 1 30 0 0000 0000 0500 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera GLA630GS 273116508208152 GAD104US UNI234LV 1/23/2006 6/21/2006 11/14/2005 11/15/2005 SampleID 53951/BenthID 35284(#counted) SampleID 52834/BenthID 35565(#counted) SampleID 49652/BenthID 35784(#counted) SampleID 53059/BenthID 36666(#counted) 0000 2000 0000

0000

0004 0000 0000 0000 0200 0000 0000 0000 0000 0000 0001 0000 1000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000As Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Plecoptera 0 A rthropoda Insecta Plecoptera

0 Arthropoda Insecta Plecoptera 0 A rthropoda Insecta Plecoptera 6 A rthropoda Insecta Plecoptera 0 A rthropoda Insecta Trichoptera 0 A rthropoda Insecta Diptera 0 Mollusca Gastropoda Mesogastropoda 0 Mollusca Gastropoda Mesogastropoda 0 Mollusca Gastropoda Mesogastropoda 0 A rthropoda Crustacea A mphipoda 0 Mollusca Gastropoda Basommatophora 0 A rthropoda Insecta Coleoptera 3 A rthropoda Insecta Coleoptera 0 A rthropoda Insecta Trichoptera 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Diptera 1 A rthropoda Insecta Odonata 0 A rthropoda Insecta Odonata 0 A rthropoda Insecta Odonata 0 A rthropoda Insecta Odonata 0 A rthropoda Insecta Odonata 0 A rthropoda A rachnida A cariformes 0 A rthropoda A rachnida A cariformes 0 A rthropoda Crustacea Decapoda 0 A rthropoda Insecta Diptera 0 A rthropoda A rachnida A cariformes 0 A rthropoda Insecta Diptera 0 A nnelida Oligochaeta Haplotaxida 0 A nnelida Oligochaeta Haplotaxida 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Ephemeroptera 0 A rthropoda Insecta Diptera 0 A rthropoda Insecta Heteroptera 0 A rthropoda Insecta Heteroptera 0 Mollusca Bivalvia Bivalvia 0 A rthropoda Insecta Collembola 0 A rthropoda Insecta Odonata 0 A rthropoda Insecta Trichoptera 0 A rthropoda Insecta Trichoptera 0 A rthropoda Insecta TrichopteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order

0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Crustacea Isopoda 0 Arthropoda Crustacea Isopoda 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Odonata 1 Arthropoda Insecta Odonata 0 Arthropoda Crustacea Decapoda 0 Mollusca Gastropoda Mesogastropoda 0 Mollusca Gastropoda Mesogastropoda 0 Arthropoda Crustacea Isopoda 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Diptera 1 Arthropoda Insecta Trichoptera 2 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera

0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Collembola 0 Arthropoda Insecta Diptera 0 Mollusca Bivalvia Veneroida 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Megaloptera

0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta DipteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Crustacea Amphipoda 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera

0 Arthropoda Insecta Trichoptera

0 Arthropoda Insecta Diptera

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera 1 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Platyhelminthes Turbellaria Tricladida 0 Platyhelminthes Turbellaria Tricladida 0 Annelida Oligochaeta Lumbriculida 0 Annelida Oligochaeta Lumbriculida 0 Arthropoda Insecta Coleoptera 0 Mollusca Gastropoda Mesogastropoda 0 Mollusca Gastropoda Mesogastropoda 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta OdonataAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order

0 Arthropoda Insecta Odonata 1 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 7 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Mollusca Gastropoda Basommatophora 0 Arthropoda Crustacea Amphipoda 0 Arthropoda Crustacea Amphipoda

0 Arthropoda Crustacea Amphipoda 0 Mollusca Gastropoda Gastropoda 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Heteroptera 0 Annelida Hirudinea Rhynchobdellida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Coleoptera 2 Arthropoda Insecta Coleoptera 0 Arthropoda Crustacea Amphipoda 0 Arthropoda Insecta Coleoptera 0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Odonata 0 Mollusca Gastropoda Basommatophora 0 Annelida Hirudinea Rhynchobdellida 0 Annelida Hirudinea Rhynchobdellida 0 Arthropoda Insecta Diptera 3 Arthropoda Insecta Diptera 0 Arthropoda Insecta Hemiptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Diptera 0 Arthropoda Crustacea Amphipoda 0 Cnidaria Hydrozoa Hydroida 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta ColeopteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Crustacea Amphipoda 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera

0 Arthropoda Insecta Trichoptera

0 Arthropoda Insecta Diptera

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera 1 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Platyhelminthes Turbellaria Tricladida 0 Platyhelminthes Turbellaria Tricladida 0 Annelida Oligochaeta Lumbriculida 0 Annelida Oligochaeta Lumbriculida 0 Arthropoda Insecta Coleoptera 0 Mollusca Gastropoda Mesogastropoda 0 Mollusca Gastropoda Mesogastropoda 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta OdonataAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 1 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Ephemeroptera 9 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Heteroptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Coleoptera 2 Arthropoda Insecta Coleoptera 0 Mollusca Gastropoda Basommatophora 0 Mollusca Gastropoda Basommatophora 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Trichoptera 0 Annelida Hirudinea Arhynchobdellida 0 Annelida Hirudinea Arhynchobdellida 0 Arthropoda Insecta Diptera 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida

0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida

0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Nemertea Nemertea Nemertea 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Arachnida AcariformesAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Arachnida Acariformes

0 Arthropoda Insecta Trichoptera

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Mollusca Gastropoda Mesogastropoda

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Diptera 4 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 1 Arthropoda Insecta Coleoptera 0 Arthropoda Arachnida Oribatei 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Diptera 0 Arthropoda Crustacea Decapoda 0 Arthropoda Crustacea Decapoda 0 Arthropoda Crustacea Decapoda

1 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta PlecopteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Heteroptera 0 Arthropoda Insecta Lepidoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Mollusca Pelecypoda Pelecypoda 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Plecoptera 0 Arthropoda Insecta Lepidoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Trichoptera 0 Mollusca Gastropoda Basommatophora 0 Mollusca Gastropoda Basommatophora 0 Arthropoda Arachnida Acariformes

0 Mollusca Bivalvia Veneroida 0 Mollusca Bivalvia Veneroida 0 Platyhelminthes Turbellaria Tricladida 0 Mollusca Gastropoda Basommatophora 0 Mollusca Gastropoda Basommatophora 0 Mollusca Gastropoda Basommatophora 0 Platyhelminthes Platyhelminthes Platyhelminthes 0 Arthropoda Insecta Plecoptera 0 Cnidaria Hydrozoa Hydroida

0 Arthropoda Insecta Trichoptera

0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta DipteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 3 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 1 Arthropoda Insecta Diptera 0 Mollusca Gastropoda Mesogastropoda 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Crustacea Decapoda 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Odonata 0 Arthropoda Insecta Coleoptera

0 Nemertea Enopla Hoplonemertea 0 Annelida Oligochaeta Haplotaxida

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera 1 Arthropoda Insecta Diptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Lepidoptera 0 Mollusca Gastropoda Mesogastropoda 0 Arthropoda Insecta Heteroptera 0 Arthropoda Insecta Heteroptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera

0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Megaloptera 0 Arthropoda Insecta DipteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Insecta Diptera 6 Arthropoda Insecta Diptera 0 Annelida Oligochaeta Haplotaxida 0 Mollusca Gastropoda Mesogastropoda 0 Arthropoda Arachnida Acariformes 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Coleoptera

0 Mollusca Bivalvia Veneroida

0 Mollusca Bivalvia Veneroida 0 Arthropoda Crustacea Isopoda 0 Arthropoda Insecta Diptera 0 Annelida Oligochaeta Haplotaxida 0 Annelida Oligochaeta Haplotaxida 3 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Coleoptera 0 Arthropoda Insecta Coleoptera 8 Arthropoda Insecta Coleoptera 15 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera

0 Arthropoda Insecta Ephemeroptera

0 Arthropoda Insecta Ephemeroptera 0 Arthropoda Insecta Diptera 0 Annelida Oligochaeta Haplotaxida 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Plecoptera 1 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Crustacea MysidaceaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera LIB104LV 11/21/2005 SampleID 49677/BenthID 36688(#counted) Phylum Class Order 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera

0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Trichoptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Ephemeroptera 0 Annelida Oligochaeta Haplotaxida 0 Platyhelminthes Turbellaria Turbellaria 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 0 Arthropoda Arachnida Acariformes 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Heteroptera 0 Arthropoda Insecta Diptera 0 Arthropoda Insecta Diptera 1 Arthropoda Insecta Diptera 0 Arthropoda Insecta ZygopteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosus Family Genus Current Name Chironomidae A blabesmyia Chironomidae A blabesmyia Chironomidae A blabesmyia Chironomidae A blabesmyia Baetidae A centrella Baetidae A cerpenna Perlidae A croneuria Perlidae A croneuria

Perlidae Acroneuria A croneuria arenosa/evoluta Perlidae A croneuria Perlidae A croneuria Sericostomatidae A garodes Ceratopogonidae A lluaudomyia Bithyniidae A mnicola Bithyniidae A mnicola Bithyniidae A mnicola A mphipoda A mphipoda A ncylidae A ncylidae Elmidae A ncyronyx Elmidae A ncyronyx Calamoceratidae A nisocentropus Culicidae A nopheles Chironomidae A psectrotanypus Coenagrionidae A rgia Coenagrionidae A rgia Coenagrionidae A rgia Coenagrionidae A rgia Coenagrionidae A rgia A rrenuridae A rrenurus A rrenuridae A rrenurus A

stacidae A stacidae Cambaridae A thericidae A therix Hygrobatidae A tractides Ceratopogonidae A trichopogon Tubificidae A ulodrilus Tubificidae A ulodrilus Baetidae Baetidae Baetidae Baetis Baetidae Baetis Baetiscidae Baetisca Chironomidae Beardius Belostomatidae Belostoma Belostomatidae Belostomatidae Bivalvia Bivalvia Sminthuridae Bourletiella A eshnidae Boyeria Brachycentridae Brachycentrus Brachycentridae Brachycentrus Brachycentridae BrachycentrusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura taris Family Genus Current Name

Caenidae Sparbarus Sparbarus maculata Asellidae Caecidotea Asellidae Caecidotea Caenidae Caenis Caenidae Caenis Caenidae Caenis Baetidae Callibaetis Calopterygidae Calopteryx Calopterygidae Calopteryx Cambaridae Cambaridae Viviparidae Campeloma Viviparidae Campeloma Sphaeromatidae Cassidinidea Cecidomyiidae Cecidomyiidae Ceratopogonidae Ceratopogonidae

Polycentropodidae Cernotina Chaoboridae Chaoborus Chaoboridae Chaoborus Corydalidae Chauliodes Empididae Chelifera Hydropsychidae Cheumatopsyche Philopotamidae Chimarra Chironomidae Chironomus Leptophlebiidae Choroterpes

Leptophlebiidae Choroterpes Choroterpes basalis Chironomidae Cladotanytarsus Chironomidae Cladotanytarsus Chironomidae Cladotanytarsus Clathrosperchonida

e Clathrosperchon Sisyridae Climacia Climacia areolaris Chironomidae Clinotanypus Chironomidae Coelotanypus Chironomidae Coelotanypus Coenagrionidae Coenagrionidae Collembola Collembola Chironomidae Conchapelopia Corbiculidae Corbicula Corduliidae Corduliidae Libellulidae Corydalidae Corydalus

Corydalidae Corydalus Corydalus cornutus Corydalidae Corydalus Chironomidae Corynoneura Chironomidae Corynoneura Chironomidae Corynoneura Chironomidae CorynoneuraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae Enallagma Family Genus Current Name Crangonyctidae Crangonyx Chironomidae Cricotopus Chironomidae Cricotopus Chironomidae Cricotopus

Chironomidae Cricotopus or orthocladius

Chironomidae Cryptochironomus Chironomidae Cryptotendipes Curculionidae Curculionidae

Polycentropodidae Cyrnellus

Chironomidae Demicryptochirono

mus

Naididae Dero Dero digitata complex Naididae Dero Naididae Dero Naididae Dero

Naididae Dero Dero digitata complex Naididae Dero Chironomidae Dicrotendipes Chironomidae Dicrotendipes Chironomidae Dicrotendipes Chironomidae Dicrotendipes Chironomidae Dicrotendipes Gyrinidae Dineutus Gyrinidae Dineutus Gyrinidae Dineutus Hydropsychidae Diplectrona Dixidae Dixa Dixidae Dixella Chironomidae Djalmabatista Dolichopodidae Dolichopodidae Gomphidae Dromogomphus Gomphidae Dromogomphus Dryopidae Dryopidae Elmidae Dubiraphia Elmidae Dubiraphia Elmidae Dubiraphia Dugesiidae Dugesia Girardia Dugesiidae Dugesia Lumbriculidae Eclipidrilus Lumbriculidae Eclipidrilus Psephenidae Ectopria Pleuroceridae Elimia Pleuroceridae Elimia Empididae Empididae Coenagrionidae EnallagmaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis Hydraenidae Family Genus Current Name

Coenagrionidae Enallagma Enallagma coecum Enchytraeidae Enchytraeidae Chironomidae Endotribelos Hydrophilidae Enochrus Ephemerellidae Ephemerellidae Ephydridae Ephydridae Libellulidae Epitheca Tetragoneuria Libellulidae Epicordulia Epicordulia regina Ephemerellidae Eurylophella Ephemerellidae Eurylophella Eurylophella doris Ancylidae Ferrissia Gammaridae Gammaridae Gammaridae Gammarus

Gammaridae Gammarus Gammarus cf. tigrinus Gastropoda Gastropoda Krendowskiidae Geayia Gerridae Gerridae Glossiphoniidae Gloiobdella Chironomidae Goeldichironomus Gomphidae Gomphidae Gomphidae Gomphus Gomphidae Gomphus Elmidae Gonielmis Elmidae Gonielmis Aoridae Grandidierella Gyrinidae Gyretes Tubificidae Haber Gomphidae Hagenius Ancylidae Hebetancylus Glossiphoniidae Helobdella Glossiphoniidae Helobdella Chironomidae Helopelopia Empididae Hemerodromia Hemiptera Hemiptera Heteroptera Heptageniidae Heptageniidae Calopterygidae Hetaerina Calopterygidae Hetaerina Calamoceratidae Heteroplectron Ephemeridae Hexagenia Ephemeridae Hexagenia Ephemeridae Hexagenia Ephemeridae Hexagenia Tipulidae Hexatoma Hyalellidae Hyalella Hydridae Hydra Hydrachnidae Hydrachna Hydraenidae Hydraena Hydraenidae HydraenidaeAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus Family Genus Current Name Hydrobiidae Hydrobiidae Hydrodromidae Hydrodroma Hydrophilidae Hydrophilidae Dytiscidae Hydroporus Hydropsychidae Hydropsyche Hydropsychidae Hydropsyche Hydropsychidae Hydropsychidae Hydroptilidae Hydroptila Hydroptilidae Hydroptilidae Hygrobatidae Hygrobates Dytiscidae Hygrotus Coenagrionidae Ischnura Coenagrionidae Ischnura Oligoneuriidae Isonychia Perlodidae Isoperla Isopoda Isopoda Isotomidae Isotomurus Isotomidae Isotomurus Chironomidae Kiefferulus Krendowskiidae Krendowskia Baetidae Labiobaetis Pseudocloeon Chironomidae Labrundinia Chironomidae Labrundinia Chironomidae Labrundinia Chironomidae Labrundinia Chironomidae Labrundinia Ancylidae Laevapex Ancylidae Laevapex Chironomidae Larsia Chironomidae Larsia Chironomidae Lauterborniella Lebertiidae Lebertia Lepidoptera Lepidoptera Leptoceridae Leptoceridae Leptophlebiidae Leptophlebia Leptophlebiidae Leptophlebiidae Leuctridae Leuctra Libellulidae Libellulidae Limnesiidae Limnesia Tubificidae Limnodrilus Tipulidae Limnophila Tipulidae Limonia Dytiscidae Liodessus

Dytiscidae Liodessus Liodessus novaffinis Dytiscidae Lioporeus Asellidae Lirceus Hydrobiidae Littoridinops Lumbriculidae Lumbriculidae Lumbriculidae LumbriculusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia Neumania Family Genus Current Name Psychomyiidae Lype Heptageniidae Maccaffertium Heptageniidae Maccaffertium Libellulidae Macromia Libellulidae Macromia Macromiidae Macromiidae Elmidae Macronychus Hydropsychidae Macrostemum Hydroptilidae Mayatrichia Hydroptilidae Mayatrichia Chironomidae Meropelopia Chironomidae Mesosmittia Gerridae Metrobates Brachycentridae Micrasema Brachycentridae Micrasema Brachycentridae Micrasema Elmidae Microcylloepus Elmidae Microcylloepus Planorbidae Micromenetus Planorbidae Micromenetus Chironomidae Microtendipes Chironomidae Microtendipes Chironomidae Microtendipes Chironomidae Microtendipes Mideopsidae Mideopsis Molannidae Molanna Erpobdellidae Mooreobdella Erpobdellidae Mooreobdella Muscidae Muscidae Naididae Naididae Naididae Nais(animal)

Naididae Nais(animal) Nais communis complex Naididae Nais(animal)

Naididae Nais(animal) Nais communis complex Naididae Nais(animal)

Naididae Nais(animal) Nais communis complex Chironomidae Nanocladius Leptoceridae Nectopsyche Leptoceridae Nectopsyche Leptoceridae Nectopsyche Leptoceridae Nectopsyche Nemertea Nemertea Perlidae Neoperla Perlidae Neoperla Dytiscidae Neoporus Hydroptilidae Neotrichia Unionicolidae NeumaniaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosa Family Genus Current Name Unionicolidae Neumania

Polycentropodidae Neureclipsis

Polycentropodidae Neureclipsis Libellulidae Neurocordulia Libellulidae Neurocordulia Libellulidae Neurocordulia Corydalidae Nigronia Corydalidae Nigronia Corydalidae Nigronia Chironomidae Nilotanypus Chironomidae Nilothauma Hydrobiidae Notogillia

Polycentropodidae Nyctiophylax Odonata Odonata Stratiomyidae Odontomyia Leptoceridae Oecetis Leptoceridae Oecetis Leptoceridae Oecetis Leptoceridae Oecetis

Leptoceridae Oecetis Oecetis sphyra/morsei Leptoceridae Oecetis Leptoceridae Oecetis Leptoceridae Oecetis Leptoceridae Oecetis Scirtidae Ora/scirtes Oribatei Oribatei Oribatida Orthocladiinae Orthocladiinae

Chironomidae Orthocladiinae gen. c epler

Chironomidae Orthocladiinae gen. f epler Chironomidae Orthocladius Hydroptilidae Orthotrichia Hydroptilidae Oxyethira Libellulidae Pachydiplax Chironomidae Pagastiella Palaemonidae Palaemonetes Palaemonidae Palaemonetes Palaemonidae Palaemonetes

Ceratopogonidae Palpomyia/bezzia grp. Chironomidae Paracladopelma Chironomidae Paracladopelma Chironomidae Paracladopelma Perlidae Paragnetina Perlidae ParagnetinaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavum Family Genus Current Name Chironomidae Parakiefferiella

Chironomidae Paralauterborniella Leptophlebiidae Paraleptophlebia

Chironomidae Parametriocnemus

Chironomidae Parametriocnemus

Polycentropodidae Paranyctiophylax Pleidae Paraplea Pyralidae Paraponyx Parapoynx Chironomidae Paratanytarsus Chironomidae Paratanytarsus Chironomidae Paratendipes Pelecypoda Pelecypoda Bivalvia Dryopidae Pelonomus Haliplidae Peltodytes Chironomidae Pentaneura Perlidae Perlesta Perlidae Perlesta Perlidae Perlesta Perlidae Perlinella Pyralidae Petrophila Chironomidae Phaenopsectra Chironomidae Phaenopsectra Dipseudopsidae Phylocentropus Physidae Physella Physa Physidae Haitia Haitia Pionidae Piona

Pisidiidae Pisidiidae Sphaeriidae(mollus ca) Pisidiidae Pisidium Planariidae Planariidae Planorbidae Planorbella Planorbidae Planorbella Planorbidae Planorbidae Platyhelminthes Platyhelminthes Plecoptera Plecoptera Plumulariidae Plumulariidae

Polycentropodidae Polycentropodidae

Polycentropodidae Polycentropus Chironomidae Polypedilum Chironomidae Polypedilum

Chironomidae Polypedilum Polypedilum flavum Chironomidae Polypedilum Chironomidae PolypedilumAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis Silvius Family Genus Current Name Chironomidae Polypedilum

Chironomidae Polypedilum Polypedilum illinoense grp. Chironomidae Polypedilum

Chironomidae Polypedilum Polypedilum scalaenum grp. Chironomidae Polypedilum Chironomidae Polypedilum Chironomidae Polypedilum Ampullariidae Pomacea Naididae Pristina Naididae Pristina Naididae Pristina Pristina jenkinae Ceratopogonidae Probezzia Cambaridae Procambarus Chironomidae Procladius

Chironomidae Procladius (holotanypus) Baetidae Procloeon Gomphidae Progomphus Gomphidae Progomphus Elmidae Promoresia

Tetrastemmatidae Prostoma Prostoma graecense Tubificidae Psammoryctides

Chironomidae Pseudochironomus Baetidae Pseudocloeon Psychodidae Psychodidae Limnephilidae Pycnopsyche Pyralidae Pyralidae Hydrobiidae Pyrgophorus Nepidae Ranatra Veliidae Rhagovelia Chironomidae Rheocricotopus Chironomidae Rheocricotopus Chironomidae Rheocricotopus Chironomidae Rheotanytarsus

Chironomidae Rheotanytarsus Rheotanytarsus pellucidus

Chironomidae Rheotanytarsus Rheotanytarsus exiguus grp. Chironomidae Rheotanytarsus Chironomidae Rheotanytarsus Scirtidae Scirtes Ora/scirtes Scirtidae Scirtidae

Ephemerellidae Teloganopsis Teloganopsis deficiens Sialidae Sialis Tabanidae SilviusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmani Family Genus Current Name Simuliidae Simuliidae Simuliidae Simulium Naididae Slavina Hydrobiidae Somatogyrus Sperchonidae Sperchon Sperchonidae Sperchonopsis Hydrophilidae Sperchopsis

Sphaeriidae Sphaeriidae Sphaeriidae(mollus ca) Sphaeriidae(mollus ca) Sphaeriidae(mollus ca) Sphaeromatidae Sphaeroma Ceratopogonidae Sphaeromias Tubificidae Spirosperma Tubificidae Spirosperma Chironomidae Stelechomyia Chironomidae Stempellina Chironomidae Stempellina Chironomidae Stempellinella Heptageniidae Stenacron Heptageniidae Stenacron Heptageniidae Stenacron Elmidae Stenelmis Elmidae Stenelmis Elmidae Stenelmis Chironomidae Stenochironomus Heptageniidae Stenonema Maccaffertium

Heptageniidae Maccaffertium Maccaffertium exiguum

Heptageniidae Maccaffertium Maccaffertium smithae Stratiomyidae Stratiomyidae Naididae Stylaria Chironomidae Synorthocladius Tabanidae Tabanus Taeniopterygidae Taeniopteryx Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Chironomidae Tanytarsus Mysidae TaphromysisAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r Zygoptera Family Genus Current Name Chironomidae Thienemanniella Chironomidae Thienemanniella Chironomidae Thienemanniella

Chironomidae Thienemanniella Thienemanniella lobapodema

Chironomidae Thienemannimyia grp. Tipulidae Tipula Tipulidae Tipulidae Torrenticolidae Torrenticola Leptoceridae Triaenodes Leptoceridae Triaenodes Leptoceridae Triaenodes Chironomidae Tribelos Chironomidae Tribelos Chironomidae Tribelos Chironomidae Tribelos Leptohyphidae Tricorythodes Tubificidae Tubificidae Turbellaria Turbellaria Chironomidae Tvetenia Chironomidae Tvetenia Unionicolidae Unionicola Chironomidae Unniella Veliidae Veliidae Chironomidae Xenochironomus Chironomidae Xestochironomus Chironomidae Xylotopus Zygoptera ZygopteraAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> A blabesmyia (karelia) grp. A blabesmyia hauberi A blabesmyia mallochi A blabesmyia rhamphe grp. A centrella parvula A cerpenna pygmaea A croneuria A croneuria abnormis

Acroneuria arenosa A croneuria arenosa/evoluta A croneuria lycorias A garodes libalis A lluaudomyia A mnicola A mnicola dalli A mnicola dalli johnsoni A mphipoda A ncylidae A ncyronyx A ncyronyx variegatus A nisocentropus pyraloides A nopheles A psectrotanypus johnsoni A rgia A rgia fumipennis A rgia moesta A rgia sedula A rgia tibialis A rrenurus A rrenurus problecornis A

stacidae A therix A tractides A trichopogon A ulodrilus pigueti A ulodrilus pluriseta Baetidae Baetis Baetis intercalaris Baetisca rogersi Beardius truncatus Belostoma Belostomatidae Bivalvia Bourletiella Boyeria vinosa Brachycentrus Brachycentrus chelatus Brachycentrus numerosusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Brachycercus maculatus Caecidotea Caecidotea racovitzai Caenis Caenis diminuta Caenis hilaris Callibaetis floridanus Calopteryx Calopteryx maculata Cambaridae Campeloma Campeloma limum Cassidinidea ovalis Cecidomyiidae Ceratopogonidae

Cernotina Chaoborus Chaoborus albatus Chauliodes Chelifera Cheumatopsyche Chimarra Chironomus Choroterpes basalis

Choroterpes hubbelli Cladotanytarsus Cladotanytarsus cf. daviesi Cladotanytarsus sp. i epler

Clathrosperchon Climacea areolaris Clinotanypus Coelotanypus Coelotanypus scapularis Coenagrionidae Collembola Conchapelopia Corbicula fluminea Corduliidae Corydalus

Corydalus cornuta Corydalus cornutus Corynoneura Corynoneura sp. b epler Corynoneura sp. c epler Corynoneura tarisAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Crangonyx Cricotopus Cricotopus bicinctus Cricotopus bicinctus grp.

Cricotopus or orthocladius

Cryptochironomus Cryptotendipes Curculionidae

Cyrnellus fraternus

Demicryptochironomus

Dero digitata Dero digitata complex Dero lodeni Dero pectinata

Dero trifida Dero vaga Dicrotendipes Dicrotendipes modestus Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. a epler Dineutus Dineutus discolor Dineutus serrulatus Diplectrona modesta Dixa Dixella Djalmabatista pulcher Dolichopodidae Dromogomphus armatus Dromogomphus spinosus Dryopidae Dubiraphia Dubiraphia quadrinotata Dubiraphia vittata Dugesia Dugesia tigrina Eclipidrilus Eclipidrilus palustris Ectopria Elimia Elimia floridensis Empididae EnallagmaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID ->

Enallagma cardenium Enchytraeidae Endotribelos hesperium Enochrus Ephemerellidae Ephydridae Epitheca Epitheca princeps regina Eurylophella doris Eurylophella temporalis Ferrissia Gammaridae Gammarus

Gammarus tigrinus Gastropoda Geayia Gerridae Gloiobdella elongata Goeldichironomus fluctuans Gomphidae Gomphus Gomphus minutus Gonielmis Gonielmis dietrichi Grandidierella bonnieroides Gyretes iricolor Haber speciosus Hagenius brevistylus Hebetancylus excentricus Helobdella stagnalis Helobdella triserialis Helopelopia Hemerodromia Hemiptera Heptageniidae Hetaerina Hetaerina titia Heteroplectron americanum Hexagenia Hexagenia bilineata Hexagenia limbata Hexagenia munda orlando Hexatoma Hyalella azteca Hydra Hydrachna Hydraena marginicollis HydraenidaeAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Lype diversa Maccaffertium Maccaffertium smithae Macromia Macromia taeniolata Macromiidae Macronychus glabratus Macrostemum carolina Mayatrichia Mayatrichia ayama Meropelopia Mesosmittia Metrobates Micrasema Micrasema rusticum Micrasema wataga Microcylloepus Microcylloepus pusillus Micromenetus Micromenetus dilatatus Microtendipes pedellus Microtendipes pedellus grp. Microtendipes rydalensis Microtendipes rydalensis grp. Mideopsis Molanna Mooreobdella Mooreobdella tetragon Muscidae Naididae Nais behningi

Nais communis Nais communis complex

Nais pardalis Nais simplex

Nais variabilis Nanocladius Nectopsyche Nectopsyche candida Nectopsyche exquisita Nectopsyche pavida Nemertea Neoperla Neoperla clymene Neoporus Neotrichia NeumaniaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Neumania distincta

Neureclipsis

Neureclipsis crepuscularis Neurocordulia Neurocordulia alabamensis Neurocordulia virginiensis Nigronia Nigronia fasciatus Nigronia serricornis Nilotanypus Nilothauma Notogillia wetherbyi

Nyctiophylax Odonata Odontomyia Oecetis Oecetis cinerascens Oecetis georgia Oecetis inconspicua cmplx.

Oecetis morsei or sphyra Oecetis parva Oecetis persimilis Oecetis sp. a floyd Oecetis sp. e floyd Ora/scirtes Oribatei Orthocladiinae

Orthocladiinae gen. c epler

Orthocladiinae gen. f epler Orthocladius annectens Orthotrichia Oxyethira Pachydiplax longipennis Pagastiella Palaemonetes Palaemonetes kadiakensis Palaemonetes paludosus

Palpomyia/bezzia grp. Paracladopelma Paracladopelma loganae Paracladopelma undine Paragnetina Paragnetina fumosaAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Parakiefferiella

Paralauterborniella nigrohalterale Paraleptophlebia volitans

Parametriocnemus

Parametriocnemus sp. f epler

Paranyctiophylax Paraplea Paraponyx Paratanytarsus sp. a epler Paratanytarsus sp. c epler Paratendipes subaequalis Pelecypoda Pelonomus obscurus Peltodytes Pentaneura inconspicua Perlesta Perlesta placida Perlesta placida complex Perlinella Petrophila Phaenopsectra obediens grp. Phaenopsectra punctipes grp. Phylocentropus Physella Physella cubensis Piona

Pisidiidae Pisidium Planariidae Planorbella duryi Planorbella scalaris Planorbidae Platyhelminthes Plecoptera Plumulariidae

Polycentropodidae

Polycentropus Polypedilum Polypedilum aviceps

Polypedilum convictum grp. Polypedilum fallax Polypedilum flavumAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Polypedilum halterale grp.

Polypedilum illinoense Polypedilum illinoense grp.

Polypedilum scalaenum Polypedilum scalaenum grp. Polypedilum sp. a epler Polypedilum tritum Pomacea paludosa Pristina aequiseta Pristina synclites Pristinella jenkinae Probezzia Procambarus Procladius
Procladius (holotanypus) Procloeon viridocularis Progomphus Progomphus obscurus Promoresia tardella

Prostoma rubrum Psammoryctides convolutus

Pseudochironomus Pseudocloeon Psychodidae Pycnopsyche Pyralidae Pyrgophorus platyrachis Ranatra kirkaldyi Rhagovelia Rheocricotopus Rheocricotopus robacki Rheocricotopus tuberculatus Rheotanytarsus

Rheotanytarsus distinctissimus grp.

Rheotanytarsus exiguus Rheotanytarsus exiguus grp. Rheotanytarsus pellucidus Scirtes Scirtidae

Serratella deficiens Sialis SilviusAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Simuliidae Simulium Slavina appendiculata Somatogyrus walkerianus Sperchon Sperchonopsis Sperchopsis

Sphaeriidae
Sphaeriidae(mollusca) Sphaeroma Sphaeromias Spirosperma Spirosperma ferox Stelechomyia perpulchra Stempellina Stempellina sp. a epler Stempellinella Stenacron Stenacron floridense Stenacron interpunctatum Stenelmis Stenelmis decorata Stenelmis hungerfordi Stenochironomus Stenonema

Stenonema exiguum

Stenonema smithae Stratiomyidae Stylaria lacustris Synorthocladius Tabanus Taeniopteryx lita Tanytarsus Tanytarsus sp. a epler Tanytarsus sp. c epler Tanytarsus sp. d epler Tanytarsus sp. e epler Tanytarsus sp. g epler Tanytarsus sp. l epler Tanytarsus sp. m epler Tanytarsus sp. o epler Tanytarsus sp. r epler Tanytarsus sp. s epler Tanytarsus sp. t epler Tanytarsus sp. u epler Taphromysis bowmaniAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Thienemanniella Thienemanniella lobapodema Thienemanniella similis

Thienemanniella sp. a epler

Thienemannimyia grp. Tipula Tipulidae Torrenticola Triaenodes Triaenodes ignitus Triaenodes perna Tribelos Tribelos atrum Tribelos fuscicornis Tribelos jucundum Tricorythodes albilineatus Tubificidae Turbellaria Tvetenia Tvetenia discoloripes grp. Unionicola Unniella multivirga Veliidae Xenochironomus xenolabis Xestochironomus Xylotopus pa r ZygopteraSampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

3105 3980 22030062 MCBRIREF MCBRIDE SLOUGH HWY.267 WAKULLA CO. Panhandle East 1028 30.236111

3682 4628 19010042 CAULKREF Calkins Ck, off Hamp Register Rd. Northeast 2264 30.330819

3797 4802 28020221 TLGRPHREF TELEGRAPH CREEK AT BABCOCK RANCH Peninsula 3236A 26.748444

3952 5024 25020111 HORSEREF Horse Creek @ SR 72 Bridge Peninsula 1787A 27.199975

6810 7823 20010454 JUNIPREF JUNIPER CREEK AT HIGHWAY 19 Peninsula 2905 29.213725

7183 8268 31010050 CRKREF CROOKED CREEK HWY.270 GADSDEN CO. Panhandle West 504 30.583275

7224 8306 33010065 RSTAREARUN Rest Area Run Creek below I 10 Panhandle West 542 30.568167

7776 8853 20030265 CECFLD5 Sal Taylor Creek below Rowell Creek Northeast 2327 30.199722

7778 8855 20030341 CECFLD7 Yellow Water Creek above Sal Taylor Creek Northeast 2323 30.200889

7779 8856 20030342 CECFLD8 Yellow Water Creek below Sal Taylor Creek Northeast 2323 30.195681

9179 10164 33010065 RSTAREARUN Rest Area Run Creek below I 10 Panhandle West 542 30.568167

9196 10181 21030064 CEDHEADREF Cedar Head Run, above Ichetucknee River Panhandle East 3519 29.980611

9206 10191 20010454 JUNIPREF JUNIPER CREEK AT HIGHWAY 19 Peninsula 2905 29.213725

9395 10288 20030341 CECFLD7 Yellow Water Creek above Sal Taylor Creek Northeast 2323 30.200889

9396 10289 20030265 CECFLD5 Sal Taylor Creek below Rowell Creek Northeast 2327 30.199722

9399 10292 20030342 CECFLD8 Yellow Water Creek below Sal Taylor Creek Northeast 2323 30.195681

10131 10723 31010050 CRKREF CROOKED CREEK HWY.270 GADSDEN CO. Panhandle West 504 30.583275

10591 11209 19010099 PIGEONREF Pigeon Creek at US 1 St. Marys-Nassau River Northeast 2105 30.761314

10633 11325 32020063 LTCRKCKREF LITTLE CROOKED CREEK S.R.79 BAY CO. Panhandle West 907 30.413444

10785 11544 28020221 TLGRPHREF TELEGRAPH CREEK AT BABCOCK RANCH Peninsula 3236A 26.748444SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

10844 11554 20020317 SILRUNREF SILVER RUN AT CONFLUENCE WITH BOAT RAMP CANAL Peninsula 2772 29.209917

11464 11963 22030062 MCBRIREF MCBRIDE SLOUGH HWY.267 WAKULLA CO. Panhandle East 1028 30.236111

11607 12057 33040064 JACKSREFB Jacks Branch, Forestry BMP Study Ref Site B Panhandle West 161 30.869528

12089 12197 26011019 LVNGSTNREF LIVINGSTON CREEK AT RUCKS DAIRY RD. Peninsula 1685B 27.7

12092 12201 31020040 TENMILEREF TEN MILE CREEK S.R.73 CALHOUN CO. Panhandle West 569 30.500353

12224 12340 26011020 TIGERREF TIGER CREEK AT WALKING WATERS RD. Peninsula 1619 27.812092

12401 12463 20030456 THOMASREFB Thomas Ck, Forestry BMP Study Ref Site B Northeast 2498 29.916

12436 12486 33010054 MCDAVREF MCDAVID CREEK AT SR 99 Panhandle West 149 30.739333

12450 12500 21010018 FALLNGREF Falling Creek @ CR 131, above falls Suwannee R. Northeast 3477 30.260964

12600 12548 26010972 DAVENPOREF DAVENPORT CR AT SR 545 BRIDGE Peninsula 3170K 28.270889

12609 12557 22020062 OKLREF Oklawaha Ck, SR 267, S of Quincy Panhandle West 811 30.450606

13475 12867 31020040 TENMILEREF TEN MILE CREEK S.R.73 CALHOUN CO. Panhandle West 569 30.500353

13535 12898 20030414 GLDHEADREF GOLD HEAD BR. IN STATE PARK Peninsula 2528 29.832231

14213 13118 33040064 JACKSREFB Jacks Branch, Forestry BMP Study Ref Site B Panhandle West 161 30.869528

14231 13136 20030456 THOMASREFB Thomas Ck, Forestry BMP Study Ref Site B Northeast 2498 29.916

14930 13379 20020012 OKLRIVREF OKLAWAHA RIVER AT SR 316 Peninsula 2740C 29.373428

14965 13401 33040014 BIGHORSREF BIG HORSE CREEK S.R.2 OKA.CO. Panhandle West 28 30.922028

14966 13403 22020062 OKLREF Oklawaha Ck, SR 267, S of Quincy Panhandle West 811 30.450606

15951 14187 25020111 HORSEREF Horse Creek @ SR 72 Bridge Peninsula 1787A 27.199975

16636 14709 28010223 NFORLOXREF N FK LOXAHATCHEE RIVER REF BIO STATION Peninsula 3224A 27

16643 14716 32020063 LTCRKCKREF LITTLE CROOKED CREEK S.R.79 BAY CO. Panhandle West 907 30.413444SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

18076 15790 21010018 FALLNGREF Falling Creek @ CR 131, above falls Suwannee R. Northeast 3477 30.260964

18372 15965 26011020 TIGERREF TIGER CREEK AT WALKING WATERS RD. Peninsula 1619 27.812092

18594 16087 28010223 NFORLOXREF N FK LOXAHATCHEE RIVER REF BIO STATION Peninsula 3224A 27

20471 17104 20030414 GLDHEADREF GOLD HEAD BR. IN STATE PARK Peninsula 2528 29.832231

20474 17107 19010099 PIGEONREF Pigeon Creek at US 1 St. Marys-Nassau River Northeast 2105 30.761314

20479 17112 21030064 CEDHEADREF Cedar Head Run, above Ichetucknee River Panhandle East 3519 29.980611

20480 17113 26010972 DAVENPOREF DAVENPORT CR AT SR 545 BRIDGE Peninsula 3170K 28.270889

21571 17864 20020012 OKLRIVREF OKLAWAHA RIVER AT SR 316 Peninsula 2740C 29.373428

22114 18267 22030010 STMARKSREF St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle East 793B 30.240222

24523 19456 33010054 MCDAVREF MCDAVID CREEK AT SR 99 Panhandle West 149 30.739333

24524 19457 33040014 BIGHORSREF BIG HORSE CREEK S.R.2 OKA.CO. Panhandle West 28 30.922028

25672 19985 22030010 STMARKSREF St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle East 793B 30.240222

26863 20697 20020317 SILRUNREF SILVER RUN AT CONFLUENCE WITH BOAT RAMP CANAL Peninsula 2772 29.209917

26865 20699 26011019 LVNGSTNREF LIVINGSTON CREEK AT RUCKS DAIRY RD. Peninsula 1685B 27.7

26868 20702 19010042 CAULKREF Calkins Ck, off Hamp Register Rd. Northeast 2264 30.330819

47698 32249 BAK210GS BAK210GS St. Mary's River - BAK210GS Northeast 2211 30.435664

48420 33507 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.555361

50131 33604 CLA254LR CLA254LR Black Creek - CLA254LR Northeast 2415C 29.979571

52996 33624 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.910805

52471 34244 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.275903

52912 34645 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.541246SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

53274 34807 21010008 HAMIL6 ALAPAHA RIVER 1 SR 150 HAMILTON Panhandle East 3324 30.599861

53732 35065 BAK210GS BAK210GS St. Mary's River - BAK210GS Northeast 2211 30.435664

53951 35284 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.813874

52834 35565 27311650820 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.521261

49652 35784 GAD104US GAD104US Quincy Creek At Sr267 - GAD104US Panhandle West 1303 30.600524

53059 36666 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.095941

49677 36688 LIB104LV LIB104LV Mule Creek - LIB104LV Panhandle West 684 30.511395

57322 36904 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.275903

57322 36904 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.275903

57324 36989 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548

57324 36989 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548

57431 36987 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.541246

57431 36987 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.541246

59773 38075 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395

59773 38075 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395 60301 38292 21202 OR006 Orange Creek Peninsula 2747 29.509568

60301 38292 21202 OR006 Orange Creek Peninsula 2747 29.509568

60308 38307 3535 SUW010 SUW010 Suwannee River Northeast 3341B 30.508115

60308 38307 3535 SUW010 SUW010 Suwannee River Northeast 3341B 30.508115

60639 38485 3546 S365 S365 Yellow River @ Hwy 2 Panhandle West 30 30.925354

60639 38485 3546 S365 S365 Yellow River @ Hwy 2 Panhandle West 30 30.925354

60751 38544 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372

60751 38544 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372

62733 39186 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.964065

62733 39186 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.964065SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

63694 39707 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548

63694 39707 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548

64191 40004 3535 SUW010 SUW010 Suwannee River Northeast 3341B 30.508115

64191 40004 3535 SUW010 SUW010 Suwannee River Northeast 3341B 30.508115

64546 40384 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3

64546 40384 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3

65025 40260 LIB104LV LIB104LV Mule Creek - LIB104LV Panhandle West 684 30.511395

65025 40260 LIB104LV LIB104LV Mule Creek - LIB104LV Panhandle West 684 30.511395

65026 40261 22020093 FLRQUINREF Quincy Creek above SR267 bridge Panhandle West 1303 30.599722

65026 40261 22020093 FLRQUINREF Quincy Creek above SR267 bridge Panhandle West 1303 30.599722

65028 40263 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545

65028 40263 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545

65029 40264 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144

65029 40264 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144

65250 40368 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483

65250 40368 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483

65291 40343 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395

65291 40343 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395

65292 40344 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.555361

65292 40344 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.555361

65293 40345 21010008 HAMIL6 ALAPAHA RIVER 1 SR 150 HAMILTON Panhandle East 3324 30.599861

65293 40345 21010008 HAMIL6 ALAPAHA RIVER 1 SR 150 HAMILTON Panhandle East 3324 30.599861

65296 40348 3546 S365 S365 Yellow River @ Hwy 2 Panhandle West 30 30.925354

65296 40348 3546 S365 S365 Yellow River @ Hwy 2 Panhandle West 30 30.925354

65306 40406 BAK208GS BAK208GS St. Mary's River - BAK208GS Northeast 2097K 30.517903SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

65306 40406 BAK208GS BAK208GS St. Mary's River - BAK208GS Northeast 2097K 30.517903

65316 40349 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.964065

65316 40349 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.964065

65379 40364 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806

65379 40364 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806

65382 40367 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278

65382 40367 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278

65414 40405 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119

65414 40405 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119

65567 40448 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3

65567 40448 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3

65636 40430 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483

65636 40430 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483

65637 40431 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.095941

65637 40431 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.095941

65715 40524 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119

65715 40524 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119

65731 40476 19010006 STMAR2REF ST MARYS RIVER AT SR #2 Northeast 2097K 30.51785

65731 40476 19010006 STMAR2REF ST MARYS RIVER AT SR #2 Northeast 2097K 30.51785

65736 40479 CLA254LR CLA254LR Black Creek - CLA254LR Northeast 2415C 29.979571

65736 40479 CLA254LR CLA254LR Black Creek - CLA254LR Northeast 2415C 29.979571

65737 40481 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.910805

65737 40481 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.910805

65739 40534 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144

65739 40534 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144SampleID Benth_IDs STORET Station Nickname Station Description Bioregion WBID Latitude

65741 40537 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545

65741 40537 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545

65891 40544 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.813874

65891 40544 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.813874

65900 40550 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372

65900 40550 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372

65907 40552 27311650820 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.521261

65907 40552 27311650820 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.521261

65931 40577 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806

65931 40577 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806

65932 40564 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278

65932 40564 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278

66028 40571 21202 OR006 Orange Creek Peninsula 2747 29.509568

66028 40571 21202 OR006 Orange Creek Peninsula 2747 29.509568SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-84.272222 3/21/1994 1 D-FrameX20 6.52 51.45 17 7 3.62

-82.241797 11/1/1994 1 D-FrameX20 3.7 29.63 14 7 13.89

-81.670333 7/27/1994 1 D-FrameX20 0 16.44 8 4 25.34

-81.987578 10/24/1994 1 D-FrameX20 0.68 29.25 9 5 12.93

-81.654861 1/31/1995 1 D-FrameX20 15.45 23.64 12 4 0

-84.880969 2/13/1995 1 D-FrameX20 0 50 15 10 4.63

-87.402944 7/12/1995 1 D-FrameX20 1.77 24.78 14 12 17.7

-81.913889 10/2/1995 1 D-FrameX20 0 58.22 12 3 1.37

-81.919542 10/2/1995 1 D-FrameX20 0 31.29 17 5 2.04

-81.918278 10/2/1995 1 D-FrameX20 0 44.66 13 5 4.85

-87.402944 2/14/1996 1 D-FrameX20 0 30.61 14 6 3.4

-82.758589 2/6/1996 1 D-FrameX20 23.15 34.26 12 1 0

-81.654861 3/4/1996 1 D-FrameX20 21.92 15.75 8 6 4.11

-81.919542 3/5/1996 1 D-FrameX20 0 11.21 7 5 13.08

-81.913889 3/5/1996 1 D-FrameX20 1.45 19.57 8 6 7.25

-81.918278 3/5/1996 1 D-FrameX20 7.23 32.53 19 7 6.02

-84.880969 7/9/1996 1 D-FrameX20 0 35.76 20 12 7.28

-81.967519 7/9/1996 1 D-FrameX20 3.82 22.29 12 4 9.55

-85.868472 7/16/1996 1 D-FrameX20 3.92 16.99 14 11 10.46

-81.670333 9/16/1996 1 D-FrameX20 0 19.63 9 8 38.32SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-81.993333 9/16/1996 1 D-FrameX20 10.24 33.13 13 7 0

-84.272222 2/11/1997 1 D-FrameX20 6.12 34.69 15 7 0.68

-86.489361 2/25/1997 1 D-FrameX20 0.71 49.29 18 5 5

-81.4 2/18/1997 1 D-FrameX20 17.31 42.31 19 3 10.9

-85.200531 2/4/1997 1 D-FrameX20 0 20.77 11 8 4.62

-81.444064 2/18/1997 1 D-FrameX20 9.91 24.32 7 7 17.12

-81.862472 7/2/1997 1 D-FrameX20 0.85 30.51 17 7 4.24

-87.448556 8/12/1997 1 D-FrameX20 0 35.43 16 11 32.28

-82.668108 7/17/1997 1 D-FrameX20 3.17 39.68 11 10 7.14

-81.591128 9/30/1997 1 D-FrameX20 4.55 28.18 14 4 11.82

-84.643581 8/12/1997 1 D-FrameX20 0 40 14 5 10.48

-85.200531 2/10/1998 1 D-FrameX20 0 16.31 11 7 4.96

-81.946289 3/10/1998 1 D-FrameX20 0.83 52.89 19 9 4.13

-86.489361 7/1/1998 1 D-FrameX20 1.16 51.45 16 7 1.73

-81.862472 2/12/1998 1 D-FrameX20 1.56 17.19 11 10 8.59

-81.901764 8/11/1998 1 D-FrameX20 4.64 8.61 9 10 5.3

-86.595167 7/15/1998 1 D-FrameX20 0 19.08 18 16 15.61

-84.643581 9/9/1998 1 D-FrameX20 1 42 14 7 12

-81.987578 2/17/1999 1 D-FrameX20 0 29.09 9 6 26.36

-80.1 3/22/1999 1 D-FrameX20 15.82 26.58 7 1 10.13

-85.868472 1/21/1999 1 D-FrameX20 3 20 10 12 2SampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-82.668108 7/27/1999 1 D-FrameX20 0.76 25 8 3 7.58

-81.444064 7/20/1999 1 D-FrameX20 19.88 13.04 7 7 19.88

-80.1 10/7/1999 1 D-FrameX20 3.23 43.55 12 4 7.26

-81.946289 1/12/2000 1 D-FrameX20 0 37.84 14 12 1.8

-81.967519 2/1/2000 1 D-FrameX20 13.51 28.83 10 6 8.11

-82.758589 3/15/2000 1 D-FrameX20 6.31 60.36 17 3 1.8

-81.591128 1/10/2000 1 D-FrameX20 5.99 32.93 9 7 9.58

-81.901764 2/15/2000 1 D-FrameX20 20.44 36.5 10 6 5.84

-84.145389 8/22/2000 1 D-FrameX20 7.41 31.48 17 3 4.63

-87.448556 2/22/2001 1 D-FrameX20 0.87 19.13 12 11 22.61

-86.595167 2/8/2001 1 D-FrameX20 0 28.38 13 10 8.78

-84.145389 9/18/2001 1 D-FrameX20 11 16 4 2 12

-81.993333 9/4/2001 1 D-FrameX20 11.27 35.91 9 4 1.41

-81.4 9/5/2001 1 D-FrameX20 1.69 26.27 11 5 20.34

-82.241797 7/10/2001 1 D-FrameX20 3.51 18.42 10 4 7.02

-82.287764 11/1/2005 1 D-FrameX20 0 25.47 5 6 6.6

-85.435 2/15/2006 1 D-FrameX20 0 40.2 15 12 6.86

-81.852289 11/14/2005 1 D-FrameX20 4.76 42.86 9 8 24.76

-81.884868 11/14/2005 1 D-FrameX20 0.94 51.89 15 7 3.77

-82.736663 12/6/2005 1 D-FrameX20 1.96 16.67 6 7 10.78

-81.953277 12/13/2005 1 D-FrameX20 1.82 21.82 9 9 20SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-83.073417 4/24/2006 1 D-FrameX20 0 50 13 7 23

-82.287764 3/20/2006 1 D-FrameX20 0.91 25.45 7 6 0.91

-81.335414 1/23/2006 1 D-FrameX20 4.55 25.45 8 1 30

-82.137561 6/21/2006 1 D-FrameX20 0 29.52 10 3 33.33

-84.580759 11/14/2005 1 D-FrameX20 0 25.23 4 6 7.48

-82.472863 11/15/2005 1 D-FrameX20 2 24 7 4 30

-84.827906 11/21/2005 1 D-FrameX20 0 22.43 6 7 15.89

-82.736663 2/21/2007 1 D-FrameX20 0 13.38 3 6 4.46

-82.736663 2/21/2007 2 D-FrameX20 0 8.81 5 7 7.55

-82.349844 2/20/2007 1 D-FrameX20 2.03 38.51 14 5 6.08

-82.349844 2/20/2007 2 D-FrameX20 3.95 42.76 17 6 3.29

-81.953277 2/26/2007 1 D-FrameX20 0 34.25 12 7 10.96

-81.953277 2/26/2007 2 D-FrameX20 0 31.33 7 7 20

-86.733732 3/26/2007 1 D-FrameX20 0 44.94 14 7 10.76

-86.733732 3/26/2007 2 D-FrameX20 0 49.66 11 6 10.34 -81.94617 3/1/2007 1 D-FrameX20 4.76 51.02 19 6 7.48

-81.94617 3/1/2007 2 D-FrameX20 2.1 48.25 15 5 6.99

-82.716569 10/10/2006 1 D-FrameX20 0 81.38 11 6 4.14

-82.716569 10/10/2006 2 D-FrameX20 0 85.82 11 5 0

-86.559172 5/15/2007 1 D-FrameX20 2.08 17.36 13 4 4.86

-86.559172 5/15/2007 2 D-FrameX20 1.4 18.88 9 7 6.99

-82.210967 5/16/2007 1 D-FrameX20 0 53.85 13 7 11.89

-82.210967 5/16/2007 2 D-FrameX20 0 49.06 11 8 14.47

-87.235993 9/19/2007 1 D-FrameX20 0 51.95 14 5 1.3

-87.235993 9/19/2007 2 D-FrameX20 0 44.59 11 5 5.1SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-82.349844 11/7/2007 1 D-FrameX20 33.75 39.38 17 0 0

-82.349844 11/7/2007 2 D-FrameX20 22.08 47.4 17 0 0

-82.716569 12/12/2007 1 D-FrameX20 0 51.25 7 5 0.63

-82.716569 12/12/2007 2 D-FrameX20 0 61.39 7 6 0.63

-82.1 5/7/2008 1 D-FrameX20 35.66 6.99 7 4 0

-82.1 5/7/2008 2 D-FrameX20 45.03 10.6 6 3 1.32

-84.827906 6/10/2008 1 D-FrameX20 0 40.82 11 5 17.01

-84.827906 6/10/2008 2 D-FrameX20 0 42.07 17 7 22.07

-84.581917 6/13/2008 1 D-FrameX20 0 43.84 14 8 9.59

-84.581917 6/13/2008 2 D-FrameX20 0.7 36.36 8 6 10.49

-84.771164 6/13/2008 1 D-FrameX20 0 19.86 12 11 4.96

-84.771164 6/13/2008 2 D-FrameX20 0 15.49 7 13 10.56

-84.861232 6/10/2008 1 D-FrameX20 0 23.84 14 9 21.19

-84.861232 6/10/2008 2 D-FrameX20 0 11.56 8 7 27.21

-81.748227 5/28/2008 1 D-FrameX20 0 47.71 20 11 9.15

-81.748227 5/28/2008 2 D-FrameX20 0 40.71 19 9 8.57

-86.733732 7/9/2008 1 D-FrameX20 0 20.55 11 9 9.59

-86.733732 7/9/2008 2 D-FrameX20 2.68 20.13 10 9 6.71

-85.435 7/24/2008 1 D-FrameX20 0 31.91 11 11 24.11

-85.435 7/24/2008 2 D-FrameX20 0 34.72 16 10 11.11

-83.073417 7/16/2008 1 D-FrameX20 0 26.95 10 7 4.26

-83.073417 7/16/2008 2 D-FrameX20 0.63 21.25 11 8 4.38

-86.559172 7/9/2008 1 D-FrameX20 0 34.23 20 10 8.05

-86.559172 7/9/2008 2 D-FrameX20 0 38.3 20 10 12.06

-82.23048 6/18/2008 1 D-FrameX20 0 32.03 11 3 22.88SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-82.23048 6/18/2008 2 D-FrameX20 0 34.21 11 3 18.42

-87.235993 7/10/2008 1 D-FrameX20 0 9.29 6 9 2.14

-87.235993 7/10/2008 2 D-FrameX20 0 21.05 8 7 1.32

-83.308472 8/12/2008 1 D-FrameX20 1.94 10.97 10 5 9.68

-83.308472 8/12/2008 2 D-FrameX20 2.61 10.46 10 4 8.5

-83.252778 8/12/2008 1 D-FrameX20 0 7.5 7 1 20

-83.252778 8/12/2008 2 D-FrameX20 0 8.51 7 0 23.4

-84.493937 6/19/2008 1 D-FrameX20 0 21.53 11 4 9.03

-84.493937 6/19/2008 2 D-FrameX20 0.71 27.66 12 4 11.35

-82.1 10/8/2008 1 D-FrameX20 14.97 36.05 8 1 6.12

-82.1 10/8/2008 2 D-FrameX20 12.14 35.71 8 2 4.29

-81.748227 10/28/2008 1 D-FrameX20 0 56.21 14 6 5.23

-81.748227 10/28/2008 2 D-FrameX20 1.38 44.83 14 6 11.03

-82.472863 10/16/2008 1 D-FrameX20 0.63 21.38 9 5 5.03

-82.472863 10/16/2008 2 D-FrameX20 2.13 21.99 7 3 0.71

-84.493937 11/13/2008 1 D-FrameX20 0 28.03 14 5 12.1

-84.493937 11/13/2008 2 D-FrameX20 1.35 24.32 15 7 12.84

-82.230314 11/12/2008 1 D-FrameX20 0.63 30.63 11 10 9.38

-82.230314 11/12/2008 2 D-FrameX20 0.65 21.43 13 11 3.9

-81.852289 11/6/2008 1 D-FrameX20 0 25.17 12 10 25.17

-81.852289 11/6/2008 2 D-FrameX20 0 30.71 11 10 16.43

-81.884868 11/6/2008 1 D-FrameX20 3.29 26.32 7 7 13.82

-81.884868 11/6/2008 2 D-FrameX20 5.3 25.17 9 11 18.54

-84.861232 11/20/2008 1 D-FrameX20 0 6.85 4 6 6.85

-84.861232 11/20/2008 2 D-FrameX20 0 7.53 5 8 9.59SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 Longitude Sample Date Nreps Gear Amphipod Pct ChironPct Chiron Taxa Clinger Taxa Coleoptera Pct

-84.771164 11/20/2008 1 D-FrameX20 0 17.01 8 9 8.84

-84.771164 11/20/2008 2 D-FrameX20 0 18.49 11 7 10.27

-81.335414 12/16/2008 1 D-FrameX20 0 33.12 7 6 18.18

-81.335414 12/16/2008 2 D-FrameX20 1.27 33.12 5 5 14.65

-82.210967 12/17/2008 1 D-FrameX20 0 49.66 7 7 6.71

-82.210967 12/17/2008 2 D-FrameX20 0 50.64 7 7 6.41

-82.137561 12/17/2008 1 D-FrameX20 0.66 44.74 9 4 31.58

-82.137561 12/17/2008 2 D-FrameX20 0.66 44.37 12 6 32.45

-83.308472 1/14/2009 1 D-FrameX20 6.25 45 15 6 9.38

-83.308472 1/14/2009 2 D-FrameX20 9.15 41.83 10 8 9.8

-83.252778 1/14/2009 1 D-FrameX20 0 53.21 14 4 8.97

-83.252778 1/14/2009 2 D-FrameX20 0 33.78 12 3 12.16

-81.94617 5/1/2008 1 D-FrameX20 0 15.6 10 5 31.91

-81.94617 5/1/2008 2 D-FrameX20 0 13.89 9 5 33.33SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

1.45 52.17 17.39 Polypedilum illinoense grp. 5.8 3 8 2.17 25.72

0 37.04 11.11 Microcylloepus pusillus 12.96 4 10 0 27.78

0.68 18.49 9.59 Dubiraphia quadrinotata 19.18 4 10 0 36.3

3.4 30.61 21.77 Polypedilum flavum 15.65 3 8 1.7 29.59

4.55 29.09 14.55 Limnodrilus hoffmeisteri 3.64 2 5 3.18 25

0.93 55.56 19.44 Rheotanytarsus exiguus grp. 0 0 8 0 19.44

0.88 28.32 12.39 Stenelmis 7.96 3 13 0 26.99

0 62.33 41.1 Polypedilum illinoense grp. 6.16 2 3 0 37.33

1.36 46.26 13.61 Palpomyia/bezzia grp. 4.08 3 6 0 28.57

0 46.6 23.3 Sphaeriidae(mollusca) 0 0 3 0 26.7

1.36 79.59 47.62 Simulium 6.12 2 4 0.34 17.35

3.7 39.81 16.67 Polypedilum illinoense grp. 1.85 1 4 6.48 29.63

0.68 20.55 12.33 Hydroptila 11.64 2 9 6.85 25.68

2.8 25.23 12.15 Bivalvia 27.1 6 9 0.93 35.98

0 22.46 28.26 Sphaeriidae(mollusca) 19.57 4 7 0.72 23.55

0.6 34.94 16.87 Sphaeriidae(mollusca) 2.41 3 5 0.9 27.41

1.32 39.74 11.26 Cheumatopsyche 8.61 7 15 0 24.5

0 24.2 14.01 Ancylidae 0 0 3 0 21.02

0.65 26.14 15.69 Chimarra 8.5 2 9 0 21.9

0.93 28.04 17.76 Microcylloepus pusillus 14.02 6 10 0.47 37.38SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

1.81 36.75 15.06 Hydropsyche 4.82 3 8 2.41 27.11

0 38.1 19.05 Cheumatopsyche 4.76 2 10 0 24.15

0 54.29 21.43 Enchytraeidae 8.57 4 9 0.36 32.5

0 42.95 17.31 Hyalella azteca 1.92 1 5 0 38.14

1.54 26.15 16.15 Perlesta 18.46 3 10 0 37.31

0 32.43 12.61 Microcylloepus pusillus 2.7 2 8 0 26.13

1.69 46.61 16.95 Leuctra 8.47 5 9 0.42 24.15

0 40.16 14.96 Stenelmis decorata 1.57 2 10 0 33.07

0 45.24 26.19 Cheumatopsyche 0.79 1 8 0 30.56

0 40.91 9.09 Microcylloepus pusillus 6.36 3 6 0 26.82

0 46.67 30.48 Enchytraeidae 2.86 2 5 0 13.33

6.38 60.99 42.55 Simulium 2.84 2 5 0 9.57

0.83 63.64 14.88 Orthocladius annectens 3.31 1 10 0 30.58

0 52.6 22.54 Leuctra 3.47 2 11 0.58 16.18

0.78 42.97 13.28 Simulium 11.72 2 10 0 21.48

0 25.83 14.57 Simulium 17.88 4 11 0.99 21.52

1.16 30.64 20.23 Cheumatopsyche 4.62 5 16 0 19.94

5 44 11 Enchytraeidae/Stenoc hironomus 10 4 10 0.5 31

0 31.82 13.64 Dubiraphia vittata 9.09 5 11 0 34.55

4.43 31.65 15.82 Hyalella azteca 15.82 2 2 2.22 32.59

1 44 18 Simulium 13 4 12 0 24.5SampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

0 25.76 28.79 Planorbidae 18.18 4 6 0 23.86

0 16.15 19.88 Hyalella azteca 6.21 3 8 0 31.68

0.81 56.45 13.71 Polypedilum illinoense grp. 25 3 3 0.4 32.26

0 41.44 9.91 Tanytarsus sp. a epler 13.51 3 14 0 21.62

0 29.73 13.51 Hyalella azteca 16.22 5 6 0 31.98

0.9 66.67 13.51 Paratanytarsus sp. c epler 0.9 1 6 1.8 30.18

0.6 43.11 19.16 Polypedilum flavum 9.58 5 10 0 29.34

2.92 43.07 20.44 Hyalella azteca 10.22 3 7 1.46 35.04

1.85 37.96 9.26 Tricorythodes albilineatus 17.59 4 7 1.39 39.81

1.74 27.83 12.17 Eurylophella doris 20 4 12 0.43 38.7

0 38.51 17.57 Cheumatopsyche 10.81 4 12 0 24.32

6 16 11 Hyalella azteca/Polypedilum illinoense grp. 11 3 5 1 30.5

0.7 35.92 24.65 Cheumatopsyche 8.45 3 7 2.82 29.23

0 26.27 14.41 Caenis 20.34 4 8 0 32.2

0 41.23 22.81 Simulium 0 0 1 0 14.04

0 28.3 38.68 Maccaffertium smithae 38.68 1 4 0 30.66

0 57.84 15.69 Simulium 13.73 5 12 0 39.22

0 43.81 27.62 Polypedilum flavum 4.76 3 8 0 35.71

0 53.77 17.92 Rheotanytarsus exiguus grp. 16.98 3 5 0 30.19

0 25.49 17.65 Lirceus 17.65 6 10 8.82 22.55

0 29.09 10.91 Microcylloepus pusillus 14.55 3 6 0.91 24.09SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

0 50 23 Stenelmis 10 4 8 0 34

0 76.36 50 Simulium 2.73 1 4 0 6.82

0 25.45 28.18 Caenis diminuta 28.18 1 3 0.45 45.45

0 30.48 17.14 Dubiraphia vittata 1.9 2 5 0 32.38

0 48.6 21.5 Simulium 26.17 3 5 0 25.7

0 24 18 Argia tibialis 4 1 4 0 23.5

0 32.71 14.02 Stenochironomus 27.1 3 9 0 38.79

0 57.32 43.31 Simulium 13.38 4 6 4.78 15.92

0 50.31 41.51 Simulium 11.95 5 10 4.4 14.15

0 39.19 20.95 Tricorythodes albilineatus 25 5 10 0 47.64

1.32 44.74 27.63 Tricorythodes albilineatus 32.24 2 7 0.33 54.61

0 50.68 21.23 Cheumatopsyche 8.22 4 7 0 25

0 43.33 20.67 Polypedilum flavum 8.67 3 8 0 28

0 52.53 18.99 Pseudochironomus 10.13 4 9 0 39.87

0 57.24 25.52 Pseudochironomus 3.45 3 9 0 42.07 0 52.38 14.29 Polypedilum flavum 12.24 6 10 0 38.78

0 51.05 9.79 Polypedilum illinoense grp./Micromenetus 9.79 4 6 0 29.37

0 81.38 26.9 Tvetenia vitracies 5.52 3 8 0 26.9

0 89.36 38.3 Tvetenia vitracies 2.13 2 6 0 25.18

0 18.75 21.53 Tricorythodes albilineatus 34.72 5 8 0 46.18

1.4 19.58 18.88 Tricorythodes albilineatus 37.76 5 9 0 46.5

0 53.85 25.17 Rheotanytarsus 6.29 3 8 0.35 23.78

0 49.69 20.75 Rheotanytarsus 6.29 5 10 1.26 25.16

0 53.25 16.23 Dicrotendipes neomodestus 21.43 5 10 0 44.48

0 45.86 16.56 Caenis eglinensis 29.3 5 10 0 49.68SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

0 43.13 33.75 Hyalella azteca 12.5 4 5 0 55

0.65 51.3 22.08 Hyalella azteca 11.04 4 5 0.32 49.35

0 55.63 37.5 Hydropsyche 0 0 3 0 28.13

0 68.99 30.38 Tvetenia vitracies 0 0 4 0 26.9

2.1 7.69 35.66 Hyalella azteca 35.66 4 7 0.35 39.16

0.66 11.92 45.03 Hyalella azteca 24.5 2 4 0.33 36.42

0.68 41.5 19.73 Leuctra 6.8 4 11 0 27.89

3.45 44.14 16.55 Leuctra 6.21 3 9 0 32.07

0.68 44.52 24.66 Ancylidae 7.53 2 4 0 21.92

0 38.46 23.08 Ancylidae 5.59 2 4 0 21.68

0.71 36.17 9.22 Maccaffertium 13.48 5 17 0 24.11

0 30.99 10.56 Maccaffertium 12.68 2 12 0 22.89

0 25.83 17.88 Stenelmis 15.89 6 15 0.99 27.15

0.68 14.29 18.37 Stenelmis 21.09 5 13 0.34 28.91

0 50.98 27.45 Polypedilum flavum 3.27 3 11 0 27.78

0.71 44.29 16.43 Polypedilum flavum 5 3 11 0 25.36

1.37 20.55 21.92 Hydroptila 13.7 5 16 4.79 17.12

1.34 22.82 25.5 Hydroptila 18.12 6 15 5.03 20.13

2.13 35.46 9.93 Rheotanytarsus exiguus grp. 7.09 4 13 0 23.4

3.47 39.58 9.03 Polypedilum flavum 4.86 4 13 0 23.26

0 31.91 26.24 Hydropsyche 6.38 3 9 0.35 19.15

0 22.5 33.75 Hydropsyche 7.5 4 8 1.25 17.19

0 40.94 11.41 Polypedilum flavum 22.15 9 14 0 31.88

1.42 46.81 16.31 Polypedilum flavum 14.89 7 14 0 31.21

0 32.68 22.22 Oecetis georgia 1.96 1 4 0 22.88SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

0 36.18 23.03 Oecetis georgia 1.32 1 5 0 18.09

0 11.43 62.14 Hydropsyche incommoda 6.43 3 8 0 11.07

0 22.37 44.74 Hydropsyche incommoda 11.84 4 9 0 19.41

0 11.61 32.9 Tricorythodes albilineatus 36.77 2 7 0 51.29

0 11.11 33.33 Tricorythodes albilineatus 37.25 3 6 0 52.29

0 8.13 40 Caenis 42.5 2 2 0 45

0 9.93 28.37 Caenis 29.79 2 2 0 47.16

0 23.61 23.61 Micromenetus 9.72 3 7 6.94 18.4

0 30.5 12.06 Cernotina/Caecidotea 10.64 3 5 6.03 20.57

2.04 37.41 14.97 Hyalella azteca 2.04 2 3 1.02 34.35

2.14 37.86 15.71 Tanytarsus sp. f epler 1.43 1 2 1.43 30

0.65 56.86 13.73 Polypedilum illinoense grp. 5.23 5 9 3.92 34.31

0.69 46.21 10.34 Caecidotea 11.03 3 7 5.17 31.03

0 27.04 40.25 Leptophlebiidae 42.77 3 5 9.43 35.53

0 29.08 48.94 Leptophlebiidae 49.65 2 2 6.03 35.46

0 30.57 8.92 Leptophlebiidae/Necto psyche exquisita 15.29 3 10 1.59 39.49

2.03 26.35 10.14 Nectopsyche exquisita 11.49 4 10 1.69 34.12

2.5 35 15.63 Hydropsyche 12.5 3 11 0.94 19.38

1.95 30.52 15.58 Hydropsyche 11.69 4 12 1.3 16.88

0 26.53 16.33 Microcylloepus pusillus 12.24 5 14 0.34 31.29

0.71 33.57 19.29 Polypedilum flavum 7.86 4 14 0.36 28.57

0.66 28.95 13.16 Triaenodes 5.26 3 10 0.66 19.74

0 28.48 16.56 Triaenodes 3.31 3 13 0.33 20.2

2.05 10.27 48.63 Eurylophella doris 60.27 7 13 3.08 37.67

2.05 8.22 41.78 Eurylophella doris 57.53 6 14 1.71 41.78SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 Decapoda Pct Diptera Pct Dominant Pct DominantPct Word Ephem Pct EphemT axa EPTTaxa FFBrowGrazP ct FFEpiCol DepPct

1.36 20.41 17.69 Taeniopteryx 21.09 6 16 0 32.31

1.37 23.97 25.34 Taeniopteryx 15.75 3 11 0 38.01

0 35.06 26.62 Cheumatopsyche/Rhe otanytarsus exiguus grp. 9.74 4 7 0 20.78

0 33.76 29.94 Rheotanytarsus exiguus grp. 14.65 3 5 0 19.43

0 51.01 33.56 Polypedilum flavum 26.17 4 8 0 35.23

0 52.56 35.9 Polypedilum flavum 20.51 2 7 0.32 33.65

1.32 45.39 29.61 Polypedilum flavum 5.92 4 8 0.66 35.86

1.32 45.03 25.83 Polypedilum flavum 4.64 3 7 0 35.43

0 50.63 22.5 Polypedilum flavum 16.88 9 11 0.94 35.63

0 47.06 24.84 Polypedilum flavum 15.03 8 14 0 35.29

0 55.77 26.28 Paratanytarsus 29.49 7 8 0.32 41.03

0 36.49 27.7 Caenis 43.24 5 6 0 39.86

1.42 17.02 17.02 Microcylloepus pusillus 6.38 4 9 0.71 23.4

1.39 18.06 19.44 Microcylloepus pusillus 6.25 5 10 1.04 24.65SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0.72 5.8 3.26 13.41 13.41 14.49 21.01 0

0 0 19.44 0 12.96 11.11 5.56 23.15 0

0 0.68 18.15 1.71 21.58 7.88 0 13.01 0.68

0 0 19.05 1.7 14.63 13.61 0 19.73 0

0 0 17.73 3.64 11.82 15.45 15.45 5 2.73

0 0 18.98 0.93 1.85 10.65 2.78 43.52 1.85

0 0 19.03 1.33 11.95 17.7 5.31 16.81 0.88

0 0 5.48 0 8.22 25.68 9.59 13.7 0

0 0 13.95 0.68 3.06 8.84 20.41 16.33 8.16

0 0 8.25 0 7.28 16.99 5.83 34.95 0

0 0 15.99 1.36 4.76 4.76 2.04 53.4 0

0 0 9.72 6.48 16.67 20.83 1.85 7.41 0.93

0 12.33 8.56 7.19 9.59 11.64 3.42 13.36 1.37

0 0 9.81 1.4 12.62 12.15 1.87 18.69 6.54

0 0 3.26 2.17 21.38 6.52 3.62 38.77 0

0 0 6.33 13.55 6.33 12.95 7.83 24.7 0

0 0 18.54 1.99 6.29 12.91 9.27 19.87 6.62

0 0 21.34 4.14 26.11 3.82 14.01 8.28 1.27

0 0 15.03 0.65 10.78 8.82 13.07 29.08 0.65

0 0 14.02 0.93 25.7 4.67 0.93 15.89 0SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 1.2 10.84 2.71 1.2 15.66 11.45 27.41 0

0 5.44 7.48 0 17.35 15.99 0.68 27.55 1.36

0 0 15.36 0.36 6.07 4.64 24.29 16.43 0

0 1.92 12.82 0 6.09 25.32 3.21 12.5 0

0 0 29.62 0.77 8.46 6.54 1.54 14.23 1.54

0 3.6 13.06 0 12.61 12.16 4.5 27.93 0

0 0 21.61 1.27 8.9 23.31 1.69 16.95 1.69

0 0 8.66 0 18.11 14.96 3.15 22.05 0

0 0 9.92 0 4.76 14.29 0 40.48 0

0 0 14.09 0 17.73 12.27 14.55 14.55 0

0 0 10 0.48 6.19 5.71 31.43 14.76 18.1

0 0 24.47 3.55 3.55 2.13 5.67 46.81 4.26

0 0 24.38 0.41 11.16 7.44 1.65 16.12 8.26

0 0 26.59 0.58 1.16 28.32 0.58 17.92 8.09

0 0 21.48 0.39 10.94 11.33 0 28.13 6.25

0 13.25 4.97 0.99 11.26 4.3 9.27 33.44 0

0 2.31 15.9 0.58 9.83 5.2 0 43.93 2.31

0 0 18 5 13 18 11 2.5 1

0.91 0 10 0 24.09 12.27 0 18.18 0

0 0.63 32.28 4.11 13.29 9.18 1.9 3.8 0

0 1 20.5 2.5 5.5 5.5 5 34.5 1SampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0 10.98 3.79 45.45 11.36 0 4.55 0

0 1.86 14.6 0 12.73 18.63 3.73 16.77 0

0 0 11.29 1.21 16.53 9.27 0.81 28.23 0

0 0 17.57 0 12.16 8.56 4.5 30.18 5.41

0 0 13.96 7.21 22.52 13.06 0.9 6.76 3.6

0 0 19.37 7.21 5.86 10.36 1.8 23.42 0

0 0 14.07 0.3 8.98 19.76 12.57 14.97 0

0 0 6.57 1.46 9.85 20.07 5.84 19.71 0

0 0 22.69 3.24 10.65 16.2 0.93 5.09 0

0 1.74 18.7 1.3 14.78 5.22 4.35 14.78 0

0 1.35 23.99 1.01 7.09 8.78 1.35 30.07 2.03

0 0 19.5 7 24.5 14 1 2.5 0

0 2.11 6.69 3.17 3.17 16.9 2.82 33.1 0

0 0 16.1 0 23.31 6.78 7.63 13.98 0

0 0.44 10.09 12.28 11.4 2.63 7.89 41.23 0

0 0 12.26 0 22.64 7.08 0 27.36 0

0 0 9.8 0 3.43 8.33 2.94 25.49 10.78

0 0 8.1 0 13.81 17.14 2.86 17.62 4.76

0 0 5.19 9.43 13.68 8.02 4.72 26.89 1.89

0 0.49 8.33 8.82 16.67 7.84 2.94 23.53 0

0 0 13.18 8.18 17.27 5 3.64 23.18 4.55SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0 14 0 16.5 12 2 21.5 0

0 0 5.91 0 1.82 3.64 12.73 69.09 0

0 0 6.82 0.45 29.09 9.55 3.64 4.55 0

0 0 9.05 0 18.57 3.33 13.33 18.57 4.76

0 0 2.8 0 16.82 7.48 1.87 44.39 0.93

0 0 34 0 17 4.5 4 17 0

0 0 11.68 0.47 18.22 21.03 0.93 8.88 0

0 0 4.46 4.78 14.65 6.69 0.64 46.82 1.27

0 0 8.18 4.4 12.26 5.66 0 50.31 0.63

0 8.78 2.36 0 10.81 10.14 0.68 15.54 4.05

0 5.26 7.57 0.66 2.3 12.83 1.97 8.55 5.92

0 0 4.79 0 10.27 13.36 0 46.58 0

0 0 5.67 0 13.67 16 0 36.67 0

0 16.46 17.72 0 9.49 2.53 1.27 6.33 6.33

0 20.69 19.66 0.69 5.86 1.03 0.69 5.17 4.14 0 0 12.59 2.04 13.61 14.63 0.68 17.01 0.68

0.7 0 8.04 4.9 19.93 12.59 1.4 20.28 2.8

0 3.45 12.76 0 6.9 3.45 0 19.66 26.9

0 3.55 8.16 0 4.26 0.71 0 19.86 38.3

0 2.78 3.13 0 35.76 4.51 0 6.94 0.69

0 4.2 3.5 1.05 30.77 2.1 0 11.89 0

0 0.7 6.99 0.35 11.19 4.55 2.1 49.3 0.7

0.31 1.26 9.43 1.26 14.78 3.77 1.26 41.51 0

0 9.09 5.19 0 5.19 8.44 0 21.75 5.84

0 7.64 2.23 0 5.41 12.1 0 17.83 5.1SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0 9.06 0 4.38 20.94 0 10 0.63

0 0 12.01 0.32 9.74 16.88 0 6.82 4.55

0 0 0.63 0 0.31 1.88 0.63 48.44 20

0 0 0.63 0 0.63 2.85 0 38.61 30.38

0 0 10.49 1.05 18.18 18.88 4.9 6.99 0

0.66 0.33 11.92 0.66 12.58 24.17 2.65 10.26 0

0 0 13.61 0.68 11.22 27.55 0.68 17.69 0.68

0 0 11.03 1.72 14.14 22.76 0 15.52 2.76

0 0 9.25 0.34 33.9 7.53 0 25.68 1.37

0 0 7.34 0.7 27.62 10.84 1.4 29.72 0.7

0 0 27.66 0.35 13.48 11.7 4.26 17.73 0.71

0 0 24.65 0 14.44 15.49 0.7 20.42 1.41

0 0 13.91 0.99 20.2 18.21 1.32 16.56 0.66

0 0 10.54 0.68 23.47 19.73 0.68 15.65 0

0 0.65 10.78 0.33 12.75 25.82 1.31 19.93 0.65

0 0.71 12.5 0.36 15 20 2.14 23.93 0

0.68 21.92 15.07 5.48 7.53 6.85 1.37 9.59 9.59

0 25.5 16.44 5.7 6.38 5.03 0 6.38 9.4

0 4.26 18.44 1.42 17.02 13.12 0.71 18.79 2.84

0 7.64 23.96 1.74 8.33 16.67 0.69 12.15 5.56

0 12.77 7.8 0.35 2.84 9.22 0 46.1 1.42

0 16.25 4.69 1.25 2.19 6.25 0.63 48.44 1.88

0 9.4 10.74 0 13.42 10.07 0.67 22.48 1.34

0 4.26 10.99 1.06 13.12 14.89 2.84 20.21 1.42

0 0 36.6 0 12.75 21.24 1.96 4.58 0SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0.66 42.43 0 11.18 17.76 3.29 6.58 0

0 6.43 2.86 0 2.5 3.57 0.71 72.86 0

0 5.92 3.29 0 3.62 6.58 0 61.18 0

0 1.29 5.48 0 21.94 2.9 0 15.16 1.94

0 5.23 7.84 0 20.26 4.58 0 8.5 1.31

0 0 15.94 0 36.88 0 0 2.19 0

0 0 11.7 0 30.85 1.42 2.84 6.03 0

0 0 15.28 7.29 34.03 2.78 0.69 4.86 9.72

0 0 30.5 6.03 18.79 3.19 0 4.26 10.64

0 1.02 6.12 2.04 8.5 9.52 23.13 14.29 0

0 0 4.64 1.79 3.57 9.64 30 14.64 4.29

0 0.65 15.03 4.25 16.99 16.01 0 8.82 0

0.34 0 16.55 5.52 21.03 14.48 0 3.79 2.07

0 0.31 8.81 9.43 22.01 2.52 0.63 11.32 0

0 0 8.16 6.03 24.82 1.77 2.13 15.6 0

0 0.64 20.06 1.59 13.06 16.24 0 4.14 3.18

0 0 21.62 2.7 13.85 13.85 0 5.41 6.76

0 0 12.5 2.19 12.5 10.94 0 41.56 0

0 0 21.1 1.95 8.44 7.14 0.65 42.53 0

0 0.68 11.56 0.34 22.11 14.63 2.04 13.61 3.4

0 4.29 15.36 0.71 14.64 22.14 0 11.79 2.14

0 0 18.75 1.64 11.18 25.33 1.97 20.72 0

0 0 17.88 0.33 15.56 25.17 0.66 19.21 0.66

0 0 5.48 4.11 31.51 5.14 0 13.01 0

0 0 3.08 2.74 30.48 6.51 0 10.96 2.74SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 FFParasite Pct FFPlPierce Pct FFPredC arnPct FFScav Pct FFScrape Pct FFShred Pct FFSubColD epPct FFSusp FiltPct FFUnknownP ct

0 0 10.88 1.02 16.33 22.11 0 17.35 0

0 0 11.64 1.03 13.36 20.55 0 15.41 0

0 0 3.25 0 14.61 6.17 0 55.19 0

0 0 2.87 0 14.65 4.46 0 58.6 0

0 0 1.01 0 18.79 19.46 0 25.5 0

0 0 2.24 0.32 16.67 19.87 0 26.92 0

0 0 4.28 0.99 21.38 19.08 1.32 15.79 0.66

0 0 3.31 0.66 20.2 22.52 1.99 15.89 0

0 0 8.13 1.56 13.75 18.44 0.63 20.31 0.63

0 0.65 9.15 0.65 11.11 20.92 0 20.92 1.31

0 0 5.13 0.64 18.59 4.81 1.28 26.92 1.28

0 0 6.08 0 30.07 1.35 2.03 19.26 1.35

0.71 0.35 14.54 0.71 30.5 5.67 0 23.4 0

0 0.69 19.44 1.04 27.43 3.47 0.69 20.83 0.69SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

9.42 0 3 2.17 14.49 2 0.72 0 0 0

0 0 1 3.71 5.56 2 0 1.85 0 0

0.68 0 4 10.27 0 0 6.16 0 0 0

1.36 0 5 11.56 0 0 1.36 0 0 0

11.82 1.82 3 10.91 15.45 2 0 0 0 0

0 0 4 1.85 2.78 2 0 13.89 0 0

0 0 9 8.84 5.31 4 0.88 11.5 0 0

4.79 0 1 0.68 9.59 5 13.01 0 0 0

8.16 0 2 2.04 20.41 4 11.56 0 0 0

6.8 0 1 0.97 5.83 3 23.3 0 0 0

0 0.68 4 1.36 2.04 2 2.04 0.68 0 0

16.67 1.85 1 2.78 1.85 1 2.78 0 0 0

6.16 2.05 1 1.37 3.42 3 0 0 0 0

6.54 0 2 0.93 1.87 1 12.15 0 0 0

7.97 1.45 1 0.72 3.62 2 30.43 0 0 0

2.41 12.65 2 0.6 7.83 4 16.87 0 0 0

0 1.32 5 1.32 9.27 3 0 1.99 0 0

21.66 3.82 2 7.64 14.01 7 7.64 0 0 0

1.96 0 6 3.27 13.07 4 2.61 1.96 0 0

0 0 4 7.48 0.93 1 4.67 0 0 0SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

0 0 4 1.81 11.45 3 1.2 0 0 0

14.29 0 1 2.04 0.68 1 0 0 0 0

1.43 0 2 0.71 24.29 2 0 0.71 0 0

0 0 2 5.77 3.21 2 5.77 0 0 0

0 0 3 0.77 1.54 2 0 41.54 0 0

0.9 0 1 0.9 4.5 4 10.81 0 0 0

3.39 0 2 5.08 1.69 2 0 16.95 0 0

0 0 6 0.79 3.15 2 0 7.87 0 0

0 0 1 0.79 0 0 0 0 0 0

1.82 0 1 4.55 14.55 6 5.45 0 0 0

0 0 1 0 31.43 2 0 0.95 0 0

0 0 3 0 5.67 3 0 14.18 0 0

0.83 0 4 3.31 1.65 2 0 4.13 0 0

0 0 5 2.31 0.58 1 0 22.54 0 0

0 0 6 5.47 0 0 0 11.72 0 0

1.99 0 2 1.32 9.27 5 10.6 0 0 0

0 0 8 1.74 0 0 0 2.89 0 0

2 2 3 0 11 1 0 8 0 0

9.09 0 1 4.55 0 0 1.82 0 0 0

0.63 1.9 1 15.82 1.9 3 0.63 0 0 0

0 2 2 3 53 0 4 0 0SampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

32.58 3.79 0 3.79 0 0 3.03 0 0 0

0 0 1 7.45 3.73 4 9.32 0 0 0

2.42 0 2 0.81 0.81 1 0 0 0 0

2.7 0 3 4.5 4.5 3 0 0.9 0 0

12.61 7.21 0 2.7 0.9 1 3.6 0 0 0

3.6 5.41 2 0.9 1.8 2 0 0 0 0

0 0 1 4.19 12.57 6 0 0 0 0

2.19 0 2 0.73 5.84 3 0.73 0 0 0

9.26 1.85 1 8.33 0.93 1 0 0 0 0

0 0 4 3.48 4.35 3 0 5.22 0 0

0 0 5 0.68 1.35 2 0.68 13.51 0 0

18 4 3 6 1 1 0 0 0 0

0 1.41 1 0 2.82 2 0 0 0 0

3.39 0 2 8.47 7.63 3 0 0 0 0

7.89 12.28 0 1.75 7.89 6 14.04 0 0 0

0 0 1 3.77 0 0 0 0 0 0

0 0 2 1.96 2.94 2 0 5.88 0 0

0 0 4 0 2.86 2 0 2.86 0 0

3.77 9.43 0 4.72 4.72 2 0 0 0 0

4.9 17.65 1 1.96 2.94 1 0.98 0 0 0

0 7.27 4 9.09 3.64 2 0.91 0 0 0SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

0 0 3 1 22 1 4 0 0

0 0 0 0.91 12.73 2 0 0 0 0

0 0.91 0 0.91 3.64 3 0 0 0 0

0.95 0 1 1.9 13.33 5 5.71 0 0 0

0 0 0 0.93 1.87 1 0 0.93 0 0

0 0 0 22 4 2 0 0 0 0

0 0 3 1.87 0.93 1 0 14.02 0 0

5.73 9.55 3 3.18 0.64 1 1.27 0 0 0

2.52 8.81 3 6.29 0 0 5.03 0 0 0

4.73 0 1 0 0.68 1 4.05 0 0 0

0 0 3 1.32 1.97 1 0.66 0 0 0

0.68 0 0 1.37 0 0 0 0 0 0

0 0 1 2.67 0 0 0 0 0 0

0 0 1 1.27 1.27 1 0 0.63 0 0

0 0.69 1 0 0.69 1 0 1.38 0 0 6.12 2.04 1 1.36 0.68 1 2.04 0 0 0

14.69 4.9 1 0.7 1.4 1 2.1 0 0 0

0 0 1 0 00 0 0 0 0

0 0 1 0.71 0 0 0 0 0 0

32.64 0 2 0.69 0 0 0.69 0 0 0

23.08 0 3 0.7 0 0 0.7 0 0 0

0.7 0.7 1 2.8 2.1 2 6.29 0 0 0

2.52 2.52 1 5.66 1.26 1 3.14 0 0 0

3.25 0 2 0 0 0 0.65 0 0 0

1.91 0 1 0 0 0 0.64 0 0 0SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

3.75 0 1 0.63 0 0 0.63 0 0 0

6.49 0 1 1.95 0 0 0 0 0 0

0 0 2 0 0.63 1 0 0 0 0

0 0 1 0 00 0 0 0 0

0.7 0 2 4.2 4.9 3 0 0 0 0

0.66 0.66 1 3.31 2.65 1 0.66 0 0 0

0 0 3 0 0.68 1 0 22.45 0 0

0 0 3 0 0 0 0 16.55 0 0

25.34 0 3 0.68 0 0 1.37 0 0 0

23.08 0 2 4.2 1.4 2 0.7 0 0 0

1.42 0 6 7.09 4.26 2 1.42 2.84 0 0

0.7 0 5 4.23 1.41 2 3.52 3.52 0 0

1.32 1.99 4 0 1.32 2 1.32 5.96 0 0

0 0.68 6 0.68 0.68 1 0.68 7.48 0 0

5.23 0 1 1.96 1.31 1 0 3.27 0 0

6.43 0 3 2.86 2.14 2 0.71 1.43 0 0

0 9.59 2 2.05 1.37 2 0 2.05 0 0

0 10.07 2 4.03 0 0 0 0 0 0

0.71 0 6 4.26 0.71 1 0 6.38 0 0

0.69 0 6 4.17 0.69 1 0 8.33 0 0

0 0.71 1 0 0 0 0.71 0 0 0

0 1.88 1 0.63 0.63 1 0 0 0 0

2.68 0 3 1.34 0.67 1 3.36 0.67 0 0

1.42 0 3 1.42 2.84 3 0 0 0 0

0.65 0 0 7.19 1.96 2 0 0 0 0SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

1.97 0 0 8.55 3.29 2 0 0 0 0

0 0 0 0.71 0.71 1 0 0 0 0

0 0 0 0.66 0 0 0 0 0 0

22.58 0 1 1.94 0 0 1.94 0 0 0

22.22 0 0 3.92 0 0 0 0 0 0

18.75 0 1 7.5 0 0 1.25 0 0 0

22.7 0 1 5.67 2.84 1 4.26 0 0 0

29.17 13.89 0 4.86 0.69 1 1.39 0 0 0

12.06 12.06 0 4.96 0 0 2.13 0 0 0

7.48 0 1 1.36 23.13 7 4.76 0 0 0

2.14 0.71 1 1.43 30 9 3.57 0 0 0

11.76 7.84 2 3.92 0 0 0 0 0 0

10.34 10.34 1 2.76 0 0 0 0 0 0

0 18.87 0 0 0.63 1 2.52 0 0 0

0 12.06 0 0 2.13 2 4.26 0 0 0

0.64 3.18 0 8.92 0 0 0.64 3.18 0 0

0 3.38 2 12.16 0 0 0 0.68 0 0

1.88 1.88 3 4.38 0 0 0 0 0 0

0.65 1.95 4 8.44 0.65 1 0 0 0 0

0.68 0.68 2 4.08 2.04 1 0 4.76 0 0

0.71 0.71 5 4.29 0 0 0.71 3.57 0 0

1.97 1.32 3 11.84 1.97 1 0 3.95 0 0

1.99 0.66 2 9.27 0.66 1 0 0.66 0 0

0 6.16 1 0 0 0 0 2.05 0 0

2.05 3.42 3 0.68 0 0 0.68 2.05 0 0SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 Gastropoda Pct Isopoda Pct LongLivedT

axa Odonata Pct Oligoc Pct Oligoc Taxa PelecypodaP ct Plecop Pct Polychaete Pct Polychaete Taxa

0 0 6 2.72 0 0 0 19.73 0 0

0 0 4 1.37 0 0 0 26.71 0 0

0 0 0 1.3 0 0 0 0 0 0

0 0 0 1.27 0 0 0.64 0 0 0

2.01 0 1 0 0 0 2.68 0 0 0

1.92 0.64 0 0 0 0 0 0 0 0

2.63 0.66 3 1.32 1.32 1 2.63 0 0 0

2.65 0 2 0.66 1.99 2 2.65 0 0 0

3.75 1.88 1 0 0.63 1 0 0 0 0

1.96 0 0 1.96 0 0 0 0 0 0

0.64 0.64 0 0.64 1.28 1 0 0 0 0

3.38 0 0 0 2.03 1 0.68 0 0 0

12.06 0 4 3.55 0 0 15.6 0 0 0

7.64 0.69 5 6.25 0.69 1 11.11 0 0 0SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

33 Excellent 5

29 Excellent 12

31 Excellent 4

31 Excellent 3

29 Excellent 4

33 Excellent 10

33 Excellent 19

25 Excellent 3

29 Excellent 6

29 Excellent 3

27 Excellent 11

27 Excellent 5

29 Excellent 8

29 Excellent 9

31 Excellent 6

31 Excellent 9

33 Excellent 19

29 Excellent 8

33 Excellent 16

31 Excellent 10SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

31 Excellent 4

33 Excellent 8

33 Excellent 9

31 Excellent 4

33 Excellent 14

31 Excellent 6

29 Excellent 10

33 Excellent 14

29 Excellent 9

29 Excellent 7

29 Excellent 12

27 Excellent 12

31 Excellent 10

29 Excellent 10

31 Excellent 12

31 Excellent 8

33 Excellent 20

29 Excellent 11

33 Excellent 3

21 Good 2

33 Excellent 14SampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

27 Excellent 6

31 Excellent 5

27 Excellent 3

33 Excellent 11

27 Excellent 10

31 Excellent 3

33 Excellent 8

33 Excellent 5

27 Excellent 5

33 Excellent 13

33 Excellent 13

19 Poor 4

29 Excellent 3

31 Excellent 4

27 Excellent 6

55 Fair 6

76 Good 18

73 Good 10

71 Fair 10

76 Good 9

82 Good 11SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

59 Fair 6

53 Fair 5

28 Poor 1

57 Fair 3

49 Fair 5

52 Fair 5

57 Fair 11

69 Category 1 A 6

69 Category 1 A 8

68 Category 1 A 5

68 Category 1 A 6

75 Category 1 A 8

75 Category 1 A 7

56 Category 2 A 10

56 Category 2 A 11 74 Category 1 A 9

74 Category 1 A 7

53 Category 2 A 3

53 Category 2 A 4

54 Category 2 A 7

54 Category 2 A 11
81 Category 1 A 4

81 Category 1 A 6

57 Category 2 A 5

57 Category 2 A 5SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

47 Category 2 A 1

47 Category 2 A 2

51 Category 2 A 4

51 Category 2 A 4

44 Category 2 A 2

44 Category 2 A 2

67 Category 2 A 13

67 Category 2 A 14

57 Category 2 A 5

57 Category 2 A 6

81 Category 1 A 16

81 Category 1 A 14

78 Category 1 A 15

78 Category 1 A 14

92 Category 1 A 16

92 Category 1 A 13

70 Category 1 A 15

70 Category 1 A 12

82 Category 1 A 12

82 Category 1 A 13

59 Category 2 A 8

59 Category 2 A 7

79 Category 1 A 13

79 Category 1 A 9

50 Category 2 A 5SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

50 Category 2 A 6

51 Category 2 A 9

51 Category 2 A 8

41 Category 2 A 3

41 Category 2 A 3

20 Category 3 A 1

20 Category 3 A 1

41 Category 2 A 8

41 Category 2 A 6

42 Category 2 A 1

42 Category 2 A 2

73 Category 1 A 13

73 Category 1 A 11

44 Category 2 A 8

44 Category 2 A 6

64 Category 2 A 9

64 Category 2 A 12

103 Category 1 A 12

103 Category 1 A 11

91 Category 1 A 14

91 Category 1 A 14

91 Category 1 A 11

91 Category 1 A 14

58 Category 2 A 8

58 Category 2 A 11SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 SCI_1992 SCI_1992 Word SCI_2004 SCI_2004 Word SCI_2007 SCI_2007 Word SCI_2007 Qualifier Sensitive Taxa

74 Category 1 A 12

74 Category 1 A 16

63 Category 2 A 3

63 Category 2 A 2

64 Category 2 A 5

64 Category 2 A 5

71 Category 1 A 6

71 Category 1 A 7

62 Category 2 A 9

62 Category 2 A 6

53 Category 2 A 9

53 Category 2 A 4

82 Category 1 A 6

82 Category 1 A 8SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

22.46 138 40 3.62 5 0 36.96 DipNet MultFactor=.08

10.19 108 37 19.44 5 0.93 3.7 DipNet GearFactor = .03

9.59 146 37 15.75 6 2.74 2.05

1.36 147 34 20.41 5 0 10.2

3.64 110 37 3.64 3 3.64 25.45 Dipnet gear factor = 0.042; H

25.93 108 34 19.44 4 0.93 1.85

5.31 113 52 15.93 6 0.88 3.54

0 146 35 0.68 1 0 47.95

2.04 147 39 3.4 3 0.68 14.29

0.97 103 28 11.65 3 0 20.39

6.8 147 35 0.68 1 2.04 4.08 

8.33 108 31 3.7 3 0.93 19.44

6.16 146 39 22.6 7 4.11 3.42

0 107 34 5.61 3 1.86 0.93

4.35 138 32 3.62 3 0.72 10.87

7.23 166 48 1.2 2 2.41 13.25

3.31 151 55 23.18 6 3.31 4.64

1.27 157 48 2.55 3 3.19 23.57

5.88 153 46 25.49 5 0 1.96

7.48 107 34 4.67 4 0 6.54SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

3.61 166 37 26.51 5 3.01 13.25

10.2 147 36 30.61 8 2.04 10.88

17.86 140 39 2.86 4 1.43 0

10.9 156 41 10.9 4 0 10.26

6.92 130 30 5.38 4 0 0

16.22 111 32 16.22 6 2.7 9.01

15.25 118 38 10.17 3 0.85 3.39 reference site b

12.6 127 40 12.6 5 1.57 3.15 Westbank @ SR 99

5.56 126 29 40.48 7 0.79 0 @ CR 131

4.55 110 39 10 3 0 3.64 Davenport @ hywy 545

5.71 105 31 4.76 2 1.9 0.95 Oklawaha

0.71 141 28 3.55 1 0.71 0.71 ten mile crk

12.4 121 43 16.53 8 0.83 0.83 Goldenhead branch @ oldmill

13.29 173 36 14.45 8 0 1.16

3.13 128 31 17.19 5 0 2.34

3.31 151 38 22.52 7 0 8.61 Oklawaha Basin Study

5.78 173 54 40.46 8 0 1.16 Bighorse crk @SR2

1 100 34 5 3 0 3 Oklawaha -SCI

9.09 110 30 16.36 6 0 16.36 SCI, BR, and qual

2.53 158 25 0 0 0 13.29 N Frk. Loxahatchee river

6 100 36 23 7 0 1 Little Crooked CreekSampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

0 132 25 4.55 2 0 29.55

6.21 161 34 15.53 5 1.86 3.11 Tiger Creek@ Walk-in-water R

22.58 124 30 0 0 0 17.74

18.92 111 40 28.83 10 1.8 0.9 LIMS427458?

2.7 111 33 1.8 1 3.6 11.71 LIMS432049?

27.93 111 38 9.01 5 2.7 9.01 LIMS442455?

8.38 167 42 11.38 5 1.2 4.79 LIMS426240?

14.6 137 34 6.57 4 0 8.03 lims435364?

4.63 108 42 4.63 3 3.7 16.67 LIMS476051?

3.48 115 43 12.17 6 0.87 1.74 LIMS510623?

4.05 148 37 22.97 7 2.03 0.68 LIMS508333?

4 100 28 2 2 11 14 lims547453?

1.41 142 25 35.92 4 0.7 12.68

12.71 118 36 8.47 4 2.54 5.93

6.14 114 32 0.88 1 1.75 16.67 532576 = Duplicate

7.55 106 17 21.7 3 0 0 SID-55672

12.75 102 36 10.78 3 0 1.96 Ref SCI,Biorecon,water,Perip h

3.81 105 24 14.29 2 0 0.95 SID-53033

28.3 106 31 1.89 2 0 7.55 SID-52997

2.94 102 35 12.75 4 0 4.9 SID-54263

4.55 110 32 11.82 3 0 2.73 SID-54275SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

17 100 27 6 3 3 4

16.36 110 18 5.45 3 0 0.91 SID-55852

4.55 110 21 1.82 2 0 11.82 SID-55073

23.81 105 31 5.71 3 1.9 8.57 not enough leaves to sample.

9.35 107 17 14.02 1 0 1.87 57811??

12 100 21 14 3 0 1 SID-53060

0.93 107 28 7.48 4 0 0.93 49678? gear factor info

0 157 25 2.55 2 0.64 3.82

0.63 159 29 6.92 5 0 2.52

10.81 148 35 17.57 5 0.68 11.49

12.5 152 36 7.89 5 0.66 9.21

7.53 146 28 26.03 3 2.05 1.37

5.33 150 25 24 5 1.33 0

5.06 158 33 20.25 4 3.16 0.63

4.14 145 27 24.83 5 1.38 0 14.97 147 45 8.84 4 1.36 12.24 LIMS # is chem

18.18 143 39 4.2 2 0 22.38 LIMS # is chem

6.9 145 20 8.97 5 0 24.14 LIMS # is chem

13.48 141 20 7.8 4 0 18.44 LIMS # is chem

4.86 144 30 5.56 3 0 11.81 LIMS # is chem

9.09 143 30 8.39 4 0 9.79 LIMS # is chem

39.86 143 33 13.99 5 1.4 4.9

39.62 159 37 11.95 5 1.26 5.66

14.94 154 31 18.83 5 0.65 18.83

10.83 157 28 17.2 5 0 10.19SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

19.38 160 33 3.13 1 1.25 6.25

21.43 154 31 3.25 1 3.25 8.44

2.5 160 14 42.5 3 0 1.25

1.27 158 13 30.38 4 0 1.27

3.5 143 27 4.9 3 0 6.99 Habitats have alot of sponge o

7.28 151 25 3.31 2 1.32 5.96 Habitats have alot of sponge o

23.13 147 34 7.48 5 2.72 5.44

22.07 145 33 7.59 5 0 6.21

19.86 146 32 8.22 2 1.37 2.05

16.08 143 31 13.99 2 0.7 1.4

4.26 141 47 21.28 9 0 3.55

0.7 142 39 26.76 7 0 2.11

3.97 151 42 24.5 5 0 0.66

2.72 147 36 25.17 5 0.68 0

9.8 153 46 22.88 7 0 1.31

10.71 140 43 27.86 7 0 2.86

2.05 146 41 38.36 10 0.68 0.68

2.01 149 39 32.89 9 0 0

12.06 141 44 15.6 6 0.71 1.42

4.86 144 43 19.44 6 2.08 2.78

5.67 141 27 51.06 6 2.13 1.42

3.13 160 31 60 4 1.25 3.13

8.05 149 53 14.77 4 2.01 4.7

6.38 141 53 17.02 7 0.71 3.55

7.19 153 28 30.07 3 0 1.31SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

8.55 152 29 29.61 4 0 0.66

0.71 140 21 78.57 5 0 0.71

4.61 152 22 63.16 5 0.66 0.66

5.16 155 32 12.26 5 1.29 3.87

3.27 153 30 11.76 3 1.96 1.31

0.63 160 24 0 0 1.88 3.75

0.71 141 22 0 0 1.42 5.67

6.25 144 34 6.94 4 0.69 25

4.26 141 33 12.77 2 2.13 9.22

16.33 147 30 0.68 1 0 49.66

17.86 140 34 0.71 1 0 53.57

9.8 153 37 6.54 4 0 15.03

2.76 145 35 5.52 4 0 6.9

5.03 159 26 1.89 2 0 1.89

4.96 141 16 0 0 0 2.84

5.73 157 41 18.47 6 6.37 1.91

6.08 148 42 22.3 5 6.76 0.68

17.5 160 38 30 8 1.25 1.88

11.04 154 41 38.31 8 0.65 1.95

2.72 147 39 20.41 7 3.4 1.36

2.14 140 42 27.14 8 1.43 0

11.84 152 34 26.32 6 0 3.29

11.26 151 37 30.46 9 0.66 0.66

4.11 146 26 11.64 5 0 0.68

4.79 146 31 13.01 6 0.68 0SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 Tanytarsini Pct TotalNind Total Taxa Trichop Pct Trichop Taxa Trombid Pct VeryTolerant Pct Sample Comments Ben/Per/Phy/M acPhy Comments

4.08 147 36 23.81 8 2.04 0

1.37 146 36 18.49 6 2.05 2.05

27.27 154 20 35.71 3 0 0.65

30.57 157 17 33.12 2 0 0

12.08 149 23 10.74 4 0.67 1.34

12.18 156 20 17.95 5 0 0

9.87 152 33 5.92 4 0 2.63

9.93 151 35 6.62 4 1.32 1.99

6.88 160 39 10 2 0 4.38

7.19 153 40 13.07 6 0 1.31

40.38 156 38 0.64 1 0.64 1.28

22.97 148 30 0.68 1 0.68 4.73

2.13 141 39 11.35 5 0 6.38

4.17 144 42 12.5 5 0.69 2.78SampleID Benth_IDs

3105 3980

3682 4628

3797 4802

3952 5024

6810 7823

7183 8268

7224 8306

7776 8853

7778 8855

7779 8856

9179 10164

9196 10181

9206 10191

9395 10288

9396 10289

9399 10292

10131 10723

10591 11209

10633 11325

10785 11544 LIMS ID

abitat Assessment score = 141SampleID Benth_IDs

10844 11554

11464 11963

11607 12057

12089 12197

12092 12201

12224 12340

12401 12463

12436 12486

12450 12500

12600 12548

12609 12557

13475 12867

13535 12898

14213 13118

14231 13136

14930 13379

14965 13401

14966 13403

15951 14187

16636 14709

16643 14716 LIMS IDSampleID Benth_IDs

18076 15790

18372 15965

18594 16087

20471 17104

20474 17107

20479 17112

20480 17113

21571 17864

22114 18267

24523 19456

24524 19457

25672 19985

26863 20697

26865 20699

26868 20702

47698 32249

48420 33507

50131 33604

52996 33624

52471 34244

52912 34645 LIMS ID

Rd.

427458

432049

442455

426240

435364

476051

510623

508333

547453

545320

545321

532576

901033SampleID Benth_IDs

53274 34807

53732 35065

53951 35284

52834 35565

49652 35784

53059 36666

49677 36688

57322 36904

57322 36904

57324 36989

57324 36989

57431 36987

57431 36987

59773 38075

59773 38075 60301 38292

60301 38292

60308 38307

60308 38307

60639 38485

60639 38485

60751 38544

60751 38544

62733 39186

62733 39186 LIMS ID

921183

931187

not provided

987267

987267

987265

987265

988483

988483

996284

996284 989694

989694

957916

957916

1009773

1009773

1047271

1047271SampleID Benth_IDs

63694 39707

63694 39707

64191 40004

64191 40004

64546 40384

64546 40384

65025 40260

65025 40260

65026 40261

65026 40261

65028 40263

65028 40263

65029 40264

65029 40264

65250 40368

65250 40368

65291 40343

65291 40343

65292 40344

65292 40344

65293 40345

65293 40345

65296 40348

65296 40348

65306 40406 LIMS ID

1062029

1062029

1073771

1073771

1101305

1101305

1107319

1107319

1108241

1108241

1108242

1108242

1107318

1107318

1104532

1104532

1114484

1114484

1118005

1118005

1115662

1115662

1114483

1114483

1109444SampleID Benth_IDs

65306 40406

65316 40349

65316 40349

65379 40364

65379 40364

65382 40367

65382 40367

65414 40405

65414 40405

65567 40448

65567 40448

65636 40430

65636 40430

65637 40431

65637 40431

65715 40524

65715 40524

65731 40476

65731 40476

65736 40479

65736 40479

65737 40481

65737 40481

65739 40534

65739 40534 LIMS ID

1109444

1114486

1114486

1123221

1123221

1123224

1123224

1109703

1109703

1138258

1138258

1143072

1143072

1140202

1140202

1147711

1147711

1147752

1147752

1146451

1146451

1146453

1146453

1149470

1149470SampleID Benth_IDs

65741 40537

65741 40537

65891 40544

65891 40544

65900 40550

65900 40550

65907 40552

65907 40552

65931 40577

65931 40577

65932 40564

65932 40564

66028 40571

66028 40571 LIMS ID

1149472

1149472

1154331

1154331

1154417

1154417

1154418

1154418

1159028

1159028

1159029

1159029

1101419

1101419SampleID HA_IDs STORET Station Nickname Station Description Bioregion WBID Latitude Longitude Sample Date

14965 646 33040014 BIGHORSREF BIG HORSE CREEK S.R.2 OKA.CO. Panhandle West 28 30.922028 -86.595167 7/15/1998

26868 683 19010042 CAULKREF Calkins Ck, off Hamp Register Rd. Northeast 2264 30.330819 -82.241797 7/10/2001

16643 723 32020063 LTCRKCKREF LITTLE CROOKED CREEK S.R.79 BAY CO. Panhandle West 907 30.413444 -85.868472 1/21/1999

18594 763 28010223 NFORLOXREF N FK LOXAHATCHEE RIVER REF BIO STATION Peninsula 3224A 27 -80.1 10/7/1999

26865 5020 26011019 LVNGSTNREF LIVINGSTON CREEK AT RUCKS DAIRY RD. Peninsula 1685B 27.7 -81.4 9/5/2001

26863 5022 20020317 SILRUNREF SILVER RUN AT CONFLUENCE WITH BOAT RAMP CANAL Peninsula 2772 29.209917 -81.993333 9/4/2001

57324 5088 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548 -82.349844 2/20/2007

57322 5089 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.275903 -82.736663 2/21/2007

57431 5097 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.541246 -81.953277 2/26/2007

64546 5552 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3 -82.1 5/7/2008

60751 5728 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372 -82.210967 5/16/2007

65026 5733 22020093 FLRQUINREF Quincy Creek above SR267 bridge Panhandle West 1303 30.599722 -84.581917 6/13/2008

65296 5755 3546 S365 S365 Yellow River @ Hwy 2 Panhandle West 30 30.925354 -86.559172 7/9/2008

65291 5756 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395 -86.733732 7/9/2008

65293 5757 21010008 HAMIL6 A LAPAHA RIVER 1 SR 150 HAMILTON Panhandle East 3324 30.599861 -83.073417 7/16/2008

65292 5759 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.555361 -85.435 7/24/2008

65029 5764 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144 -84.861232 6/10/2008

65316 5824 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.964065 -87.235993 7/10/2008

65028 5825 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545 -84.771164 6/13/2008

65025 5826 LIB104LV LIB104LV Mule Creek - LIB104LV Panhandle West 684 30.511395 -84.827906 6/10/2008

65379 5830 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806 -83.308472 8/12/2008

65382 5831 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278 -83.252778 8/12/2008

65414 5834 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119 -84.493937 6/19/2008SampleID HA_IDs STORET Station Nickname Station Description Bioregion WBID Latitude Longitude Sample Date

65567 5857 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3 -82.1 10/8/2008

65636 5927 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483 -81.748227 10/28/2008

65737 5929 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.910805 -81.884868 11/6/2008

65736 5930 CLA254LR CLA254LR Black Creek - CLA254LR Northeast 2415C 29.979571 -81.852289 11/6/2008

65637 5939 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.095941 -82.472863 10/16/2008

65731 5946 19010006 STMAR2REF ST MARYS RIVER AT SR #2 Northeast 2097K 30.51785 -82.230314 11/12/2008

65900 6011 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.473372 -82.210967 12/17/2008

65907 6013 2731165082 0 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.521261 -82.137561 12/17/2008

65891 6022 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.813874 -81.335414 12/16/2008

65932 6024 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.890278 -83.252778 1/14/2009 66028 6026 21202 OR006 Orange Creek Peninsula 2747 29.509568 -81.94617 5/1/2008

65250 6036 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.007483 -81.748227 5/28/2008

65306 6037 BAK208GS BAK208GS St. Mary's River - BAK208GS Northeast 2097K 30.517903 -82.23048 6/18/2008

65931 6056 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.828806 -83.308472 1/14/2009

65741 6063 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle West 626 30.534545 -84.771164 11/20/2008

65715 6064 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.130119 -84.493937 11/13/2008

65739 6066 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.450144 -84.861232 11/20/2008

48420 7144 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.555361 -85.435 2/15/2006

52834 7254 2731165082 0 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.521261 -82.137561 6/21/2006 60301 7531 21202 OR006 Orange Creek Peninsula 2747 29.509568 -81.94617 3/1/2007

64191 7638 3535 SUW010 SUW010 Suwannee River Northeast 3341B 30.508115 -82.716569 12/12/2007

59773 7701 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.833395 -86.733732 3/26/2007

63694 7751 3513 FLO521150 Withlacoochee River @ Stokes Ferry Peninsula 1329C 28.988548 -82.349844 11/7/2007SampleID HA_IDs

14965 646

26868 683

16643 723

18594 763

26865 5020

26863 5022

57324 5088

57322 5089

57431 5097

64546 5552

60751 5728

65026 5733

65296 5755

65291 5756

65293 5757

65292 5759

65029 5764

65316 5824

65028 5825

65025 5826

65379 5830

65382 5831

65414 5834 Artificial Channeli

zation Bank Stability

- left bank Bank Stability - right bank Habitat Score Habitat Smothering Primary Score Riparian Buffer Zone Width - left bank Riparian Buffer Zone Width - right bank Ri par i an Zone Vegetation Quality - left bank Ri par i an Zone Vegetation

Quality - right bank

19 9 9 132 7 55 10 10 10 10

20 5 5 102 15 48 3 3 9 9

20 10 10 143 16 70 10 5 9 9

20 10 10 160 20 80 10 10 10 10

20 9 7 128 14 57 10 7 9 9

20 9 9 146 17 68 10 10 10 10

20 10 10 153 18 74 10 9 10 10

20 9 10 138 17 59 10 10 10 10

20 6 6 131 12 59 10 10 10 10

20 10 10 151 20 71 10 10 10 10

14 7 7 106 11 42 9 9 9 9

20 8 8 122 13 50 10 10 8 8

20 8 9 137 18 60 10 10 10 10

20 8 8 128 15 52 10 10 10 10

20 8 10 138 16 60 10 10 10 10

20 8 5 130 16 57 10 10 10 10

20 10 8 131 16 53 10 10 10 10

20 9 5 120 17 46 10 10 10 10

20 9 9 137 18 59 10 10 10 10

20 9 9 132 13 54 10 10 10 10

20 10 10 151 17 71 10 10 10 10

20 7 7 130 17 56 10 10 10 10

20 8 8 128 13 52 10 10 10 10SampleID HA_IDs

65567 5857

65636 5927

65737 5929

65736 5930

65637 5939

65731 5946

65900 6011

65907 6013

65891 6022

65932 6024 66028 6026

65250 6036

65306 6037

65931 6056

65741 6063

65715 6064

65739 6066

48420 7144

52834 7254 60301 7531

64191 7638

59773 7701

63694 7751 Artificial Channeli

zation Bank Stability

- left bank Bank Stability - right bank Habitat Score Habitat Smothering Primary Score Riparian Buffer Zone Width - left bank Riparian Buffer Zone Width - right bank Riparian Zone Vegetation Quality - left bank Riparian Zone Vegetation

Quality - right bank

19 10 9 137 17 59 10 10 10 10

20 10 10 148 15 68 10 10 10 10

19 8 7 144 19 70 10 10 10 10

20 7 7 133 17 59 10 10 10 10

20 8 8 118 19 42 10 10 10 10

20 8 10 124 17 48 10 10 9 9

20 6 7 109 11 38 10 10 9 9

20 9 9 128 16 52 10 10 9 9

20 9 9 134 19 58 10 10 9 9

20 10 10 126 14 46 10 10 10 10 20 7 8 127 16 52 10 10 10 10

20 8 8 143 19 67 10 10 10 10

19 6 6 114 18 43 10 10 10 10

20 10 10 145 14 65 10 10 10 10

20 9 9 133 17 55 10 10 10 10

20 8 8 121 16 46 10 9 10 10

20 10 9 134 15 55 10 10 10 10

20 8 8 125 7 49 10 10 10 10

20 9 9 128 15 52 10 10 9 9 20 7 7 110 11 40 10 10 8 8

20 10 10 144 18 66 10 10 9 9

19 3 9 103 11 40 9 8 8 7

19 9 9 114 11 45 8 8 8 8SampleID HA_IDs

14965 646

26868 683

16643 723

18594 763

26865 5020

26863 5022

57324 5088

57322 5089

57431 5097

64546 5552

60751 5728

65026 5733

65296 5755

65291 5756

65293 5757

65292 5759

65029 5764

65316 5824

65028 5825

65025 5826

65379 5830

65382 5831

65414 5834 Secondary Score Substrate Availability Substrate Diversity Water Velocity

77 15 15 18

54 10 13 10

73 19 15 20

80 20 20 20

71 16 12 15

78 18 14 19

79 19 19 18

79 9 15 18

72 15 17 15

80 20 18 13

64 4 10 17

72 8 13 16

77 8 14 20

76 8 9 20

78 18 9 17

73 9 14 18

78 7 12 18

74 4 5 20

78 10 13 18

78 9 14 18

80 20 19 15

74 18 17 4

76 8 17 14SampleID HA_IDs

65567 5857

65636 5927

65737 5929

65736 5930

65637 5939

65731 5946

65900 6011

65907 6013

65891 6022

65932 6024 66028 6026

65250 6036

65306 6037

65931 6056

65741 6063

65715 6064

65739 6066

48420 7144

52834 7254 60301 7531

64191 7638

59773 7701

63694 7751 Secondary Score Substrate Availability Substrate Diversity Water Velocity

78 12 18 12

80 18 18 17

74 18 18 15

74 9 15 18

76 8 13 2

76 7 10 14

71 5 8 14

76 9 13 14

76 14 15 10

80 13 13 6 75 7 14 15

76 11 19 18

71 4 10 11

80 18 14 19

78 8 13 17

75 10 16 4

79 8 14 18

76 7 15 20

76 8 14 15 70 3 13 13

78 16 12 20

63 3 8 18

69 11 8 15SampleID HA_IDs

14965 646

26868 683

16643 723

18594 763

26865 5020

26863 5022

57324 5088

57322 5089

57431 5097

64546 5552

60751 5728

65026 5733

65296 5755

65291 5756

65293 5757

65292 5759

65029 5764

65316 5824

65028 5825

65025 5826

65379 5830

65382 5831

65414 5834 Sample Comments

Bighorse crk @SR2

532576 = Duplicate

Little Crooked Creek

Habitats have alot of sponge on themSampleID HA_IDs

65567 5857

65636 5927

65737 5929

65736 5930

65637 5939

65731 5946

65900 6011

65907 6013

65891 6022

65932 6024 66028 6026

65250 6036

65306 6037

65931 6056

65741 6063

65715 6064

65739 6066

48420 7144

52834 7254 60301 7531

64191 7638

59773 7701

63694 7751 Sample Comments

Ref SCI,Biorecon,water,Periphyton samples taken,SCI shipped to Tall.,Turb. viola

not enough leaves to sample. Neoperla observedSampleID HA_IDs

14965 646

26868 683

16643 723

18594 763

26865 5020

26863 5022

57324 5088

57322 5089

57431 5097

64546 5552

60751 5728

65026 5733

65296 5755

65291 5756

65293 5757

65292 5759

65029 5764

65316 5824

65028 5825

65025 5826

65379 5830

65382 5831

65414 5834 Ben/Per/Phy/MacPhy Comments LIMS ID FieldComment1 FieldComment2SampleID HA_IDs

65567 5857

65636 5927

65737 5929

65736 5930

65637 5939

65731 5946

65900 6011

65907 6013

65891 6022

65932 6024 66028 6026

65250 6036

65306 6037

65931 6056

65741 6063

65715 6064

65739 6066

48420 7144

52834 7254 60301 7531

64191 7638

59773 7701

63694 7751 Ben/Per/Phy/MacPhy Comments LIMS ID FieldComment1 FieldComment2

both banks sand/rock. Stream bed limestone.A s Entered / STORET station -> 22030010 22030010 32030023 Sample date -> 8/22/2000 9/18/2001 2/15/2006

Taxon Name / SampleID/PerID -> Sampleid 22114/PerID 8421 (# counted) Sampleid 25672/PerID 10528 (# counted) Sampleid 48420/PerID 18531 (# counted) Phylum A chnanthes 1

- 3 Bacillariophyta A chnanthes clevei

- 19 - Bacillariophyta A chnanthes exigua 3 3 - Bacillariophyta A chnanthes hungarica

- 2 1 Bacillariophyta A chnanthes lanceolata 64 5 - Bacillariophyta A chnanthes lanceolata apiculata

- - 1 Bacillariophyta A chnanthes minutissima

- - 6 Bacillariophyta A mphipleura pellucida pellucida 3

- - Bacillariophyta A mphora 1 2 - Bacillariophyta A nkistrodesmus falcatus 1

- - Chlorophycota Bacillaria paradoxa 1 3 - Bacillariophyta Bacillariophyceae

- - 1 Bacillariophyta Chlorella

- 2 3 Chlorophycota Cocconeis disculus

- 2 - Bacillariophyta Cocconeis fluviatilis 10

- - Bacillariophyta Cocconeis placentula 58 195 1 Bacillariophyta Coscinodiscus

- 2 - Bacillariophyta Cosmarium

- - 2 Chlorophycota Cyanobium parvum

- 3 - Cyanophycota Cyanobium plancticum

- 3 - Cyanophycota Cyclotella 4 1 - Bacillariophyta Cymbella

- 12 - Bacillariophyta Cymbella tumida tumida 10

- - Bacillariophyta Cymbellaceae

- - 7 Bacillariophyta Desmococcus

- - 4 Chlorophycota Diadesmis contenta

- - 1 Bacillariophyta Diatoma

- 2 - Bacillariophyta Encyonema

- - 3 Bacillariophyta Encyonema evergladianum

- 5 - Bacillariophyta Eunotia 135 8 191 Bacillariophyta Fragilaria

- - 1 Bacillariophyta Fragilaria pinnata

- 15 - Bacillariophyta Fragilariaceae

- 13 - Bacillariophyta Frustulia rhomboides

- - 18 Bacillariophyta Gomphonema 7 8 5 Bacillariophyta Gomphonema gracile

- - 1 Bacillariophyta Gomphonema parvulum 9 5 - Bacillariophyta Gomphonema subclavatum 4

- - Bacillariophyta Gomphonema truncatum

- 7 - Bacillariophyta Gomphonema truncatum truncatum 1

- - Bacillariophyta Hydrosera whampoensis 11

- - Bacillariophyta Jaaginema

- - 5 Cyanophycota Kephyrion

- 1 - Chrysophyta Komvophoron schmidlei

- - 1 Cyanophycota Lyngbya 4 2 - Cyanophycota Melosira 24

- - Bacillariophyta Microthamnion kuetzingianum

- - 2 Chlorophycota Navicula 1

- 3 Bacillariophyta Navicula constans

- 1 - Bacillariophyta Navicula cryptocephala 12 9 4 Bacillariophyta Navicula cryptotenella

- 12 2 BacillariophytaA s Entered / STORET station -> 22030010 22030010 32030023 Sample date -> 8/22/2000 9/18/2001 2/15/2006

Taxon Name / SampleID/PerID -> Sampleid 22114/PerID 8421 (# counted) Sampleid 25672/PerID 10528 (# counted) Sampleid 48420/PerID 18531 (# counted) Phylum Navicula minima 6 42 1 Bacillariophyta Navicula pupula

- 7 - Bacillariophyta Navicula radiosa 1

- - Bacillariophyta Nitzschia 4

- 6 Bacillariophyta Nitzschia amphibia 29 32 - Bacillariophyta Nitzschia palea 7 9 1 Bacillariophyta Oscillatoria 6 7 - Cyanophycota Pinnularia

- - 6 Bacillariophyta Planktolyngbya

- - 3 Cyanophycota Planktothrix

- - 1 Cyanophycota Pleurocapsa

- - 1 Cyanophycota Pseudanabaena

- - 8 Cyanophycota Scenedesmus acutiformis

- - 1 Chlorophycota Scenedesmus bijuga

- - 1 Chlorophycota Staurastrum

- - 2 Chlorophycota Stauroneis

- - 2 Bacillariophyta Stigeoclonium

- - 1 Chlorophycota Surirella

- - 5 Bacillariophyta Synechococcus 3

- - Cyanophycota Synechocystis

- - 3 Cyanophycota Synedra 98 18 - Bacillariophyta Synedra ulna

- 41 - Bacillariophyta Synedra ulna ramesi 19

- - Bacillariophyta Thalassiosira

- - 1 BacillariophytaA s Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes minutissima A mphipleura pellucida pellucida A mphora A nkistrodesmus falcatus Bacillaria paradoxa Bacillariophyceae Chlorella Cocconeis disculus Cocconeis fluviatilis Cocconeis placentula Coscinodiscus Cosmarium Cyanobium parvum Cyanobium plancticum Cyclotella Cymbella Cymbella tumida tumida Cymbellaceae Desmococcus Diadesmis contenta Diatoma Encyonema Encyonema evergladianum Eunotia Fragilaria Fragilaria pinnata Fragilariaceae Frustulia rhomboides Gomphonema Gomphonema gracile Gomphonema parvulum Gomphonema subclavatum Gomphonema truncatum Gomphonema truncatum truncatum Hydrosera whampoensis Jaaginema Kephyrion Komvophoron schmidlei Lyngbya Melosira Microthamnion kuetzingianum Navicula Navicula constans Navicula cryptocephala Navicula cryptotenella Class Order Family Genus Bacillariophyceae A chnanthales A chnanthaceae A chnanthes Bacillariophyceae A chnanthales A chnanthidiaceae Karayevia Bacillariophyceae A chnanthales A chnanthaceae A chnanthes Bacillariophyceae A chnanthales A chnanthidiaceae Lemnicola Bacillariophyceae A chnanthales A chnanthidiaceae Planothidium Bacillariophyceae A chnanthales A chnanthidiaceae Planothidium Bacillariophyceae A chnanthales A chnanthidiaceae A chnanthidium Bacillariophyceae Naviculales A mphipleuraceae A mphipleura Bacillariophyceae Thalassiophysales Catenulaceae A mphora Chlorophyceae Chlorococcales Oocystaceae A nkistrodesmus Bacillariophyceae Bacillariales Bacillariaceae Bacillaria Bacillariophyceae Bacillariophyceae Bacillariophyceae Bacillariophyceae Chlorophyceae Chlorococcales Oocystaceae Chlorella Bacillariophyceae A chnanthales Cocconeidaceae Cocconeis Bacillariophyceae A chnanthales Cocconeidaceae Cocconeis Bacillariophyceae A chnanthales Cocconeidaceae Cocconeis Coscinodiscophyceae Coscinodiscales Coscinodiscaceae Coscinodiscus Chlorophyceae Zygnematales Desmidiaceae Cosmarium Cyanophyceae Chroococcales Synechococcaceae Cyanobium Cyanophyceae Chroococcales Synechococcaceae Cyanobium Coscinodiscophyceae Thalassiosirales Stephanodiscaceae Cyclotella Bacillariophyceae Cymbellales Cymbellaceae Cymbella Bacillariophyceae Cymbellales Cymbellaceae Cymbella Bacillariophyceae Cymbellales Cymbellaceae Cymbellaceae Chlorophyceae Chaetophorales Chaetophoraceae Desmococcus Bacillariophyceae Naviculales Diadesmidaceae Diadesmis Fragilariophyceae Fragilariales Fragilariaceae Diatoma Bacillariophyceae Cymbellales Cymbellaceae Encyonema Bacillariophyceae Cymbellales Cymbellaceae Encyonema Bacillariophyceae Eunotiales Eunotiaceae Eunotia Fragilariophyceae Fragilariales Fragilariaceae Fragilaria Fragilariophyceae Fragilariales Fragilariaceae Staurosirella Fragilariophyceae Fragilariales Fragilariaceae Fragilariaceae Bacillariophyceae Naviculales A mphipleuraceae Frustulia Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Bacillariophyceae Cymbellales Gomphonemataceae Gomphonema Coscinodiscophyceae Biddulphiales Biddulphiaceae Hydrosera Cyanophyceae Oscillatoriales Pseudanabaenaceae Jaaginema Chrysophyceae Chromalinales Chrysococcaceae Kephyrion Cyanophyceae Oscillatoriales Borziaceae Komvophoron Cyanophyceae Oscillatoriales Oscillatoriaceae Lyngbya Coscinodiscophyceae Melosirales Melosiraceae Melosira Chlorophyceae Chaetophorales Chaetophoraceae Microthamnion Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Naviculales Naviculaceae NaviculaA s Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Navicula minima Navicula pupula Navicula radiosa Nitzschia Nitzschia amphibia Nitzschia palea Oscillatoria Pinnularia Planktolyngbya Planktothrix Pleurocapsa Pseudanabaena Scenedesmus acutiformis Scenedesmus bijuga Staurastrum Stauroneis Stigeoclonium Surirella Synechococcus Synechocystis Synedra Synedra ulna Synedra ulna ramesi Thalassiosira Class Order Family Genus Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Naviculales Naviculaceae Navicula Bacillariophyceae Bacillariales Bacillariaceae Nitzschia Bacillariophyceae Bacillariales Bacillariaceae Nitzschia Bacillariophyceae Bacillariales Bacillariaceae Nitzschia Cyanophyceae Oscillatoriales Oscillatoriaceae Oscillatoria Bacillariophyceae Naviculales Pinnulariaceae Pinnularia Cyanophyceae Oscillatoriales Pseudanabaenaceae Planktolyngbya Cyanophyceae Oscillatoriales Phormidiaceae Planktothrix Cyanophyceae Pleurocapsales Pleurocapsaceae Pleurocapsa Cyanophyceae Oscillatoriales Pseudanabaenaceae Pseudanabaena Chlorophyceae Chlorococcales Scenedesmaceae Scenedesmus Chlorophyceae Chlorococcales Scenedesmaceae Scenedesmus Chlorophyceae Zygnematales Desmidiaceae Staurastrum Bacillariophyceae Naviculales Stauroneidaceae Stauroneis Chlorophyceae Chaetophorales Chaetophoraceae Stigeoclonium Bacillariophyceae Surirellales Surirellaceae Surirella Cyanophyceae Chroococcales Chroococcaceae Synechococcus Cyanophyceae Chroococcales Chroococcaceae Synechocystis Fragilariophyceae Fragilariales Fragilariaceae Synedra Fragilariophyceae Fragilariales Fragilariaceae Synedra Fragilariophyceae Fragilariales Fragilariaceae Synedra Coscinodiscophyceae Thalassiosirales Thalassiosiraceae ThalassiosiraA s Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes minutissima A mphipleura pellucida pellucida A mphora A nkistrodesmus falcatus Bacillaria paradoxa Bacillariophyceae Chlorella Cocconeis disculus Cocconeis fluviatilis Cocconeis placentula Coscinodiscus Cosmarium Cyanobium parvum Cyanobium plancticum Cyclotella Cymbella Cymbella tumida tumida Cymbellaceae Desmococcus Diadesmis contenta Diatoma Encyonema Encyonema evergladianum Eunotia Fragilaria Fragilaria pinnata Fragilariaceae Frustulia rhomboides Gomphonema Gomphonema gracile Gomphonema parvulum Gomphonema subclavatum Gomphonema truncatum Gomphonema truncatum truncatum Hydrosera whampoensis Jaaginema Kephyrion Komvophoron schmidlei Lyngbya Melosira Microthamnion kuetzingianum Navicula Navicula constans Navicula cryptocephala Navicula cryptotenella Current Name

Karayevia clevei A chnanthidium exiguum Lemnicola hungarica Planothidium lanceolatum Planothidium apiculatum A chnanthidium minutissimum

Bacillaria paxillife r

Staurosirella pinnataA s Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Navicula minima Navicula pupula Navicula radiosa Nitzschia Nitzschia amphibia Nitzschia palea Oscillatoria Pinnularia Planktolyngbya Planktothrix Pleurocapsa Pseudanabaena Scenedesmus acutiformis Scenedesmus bijuga Staurastrum Stauroneis Stigeoclonium Surirella Synechococcus Synechocystis Synedra Synedra ulna Synedra ulna ramesi Thalassiosira Current Name

Sellaphora pupulaAs Entered / STORET station -> GAD104US LIB104LV CLA243LV CLA254LR UNI234LV LEV502GS Sample date -> 11/14/2005 11/21/2005 11/14/2005 11/14/2005 11/15/2005 12/6/2005

Taxon Name / SampleID/PerID -> Sampleid 49652/PerID

18430 (# counted) Sampleid 49677/PerID

18448 (# counted) Sampleid 52996/PerID

19568 (# counted) Sampleid 50131/PerID

19608 (# counted) Sampleid 53059/PerID

19639 (# counted) Sampleid 52471/PerID

20006 (# counted) A ctinotaenium

------ A ctinotaenium perminutum

------ A nabaena

------ A nabaena subcylindrica

------ A nkistrodesmus

- - 1

- - - A nkistrodesmus falcatus

- 5

---- A phanocapsa

- 1

---- Bacillariophyta 262 213 283 247 295 269 Batrachospermum

------ Bumilleriopsis brevis

------ Calothrix

------ Chlamydomonas -----1 Chlorella

- 6

- - - 3 Chlorella vulgaris

------ Chlorococcum

- 2 1

- - 2 Chlorococcum humicola

------ Chlorogonium

------ Chlorophyceae

- 4

---- Chroococcus

------ Chroococcus minutus

------ Chroococcus turgidus

------ Chrysophyceae

------ Cladophora

- 4

---- Closterium ----1- Closterium gracile gracile

------ Closterium lanceolatum lanceolatum

------ Closterium moniliferum moniliferum

------ Closterium parvulum obtusum

------ Closterium peracerosum

------ Closterium venus venus

------ Cosmarium 3 1

---- Crucigenia tetrapedia

------ Cryptomonas

------ Cyanobium

------ Cyanobium parvum

- 9

---- Cyanobium plancticum

------ Cyanophyceae

------ Cyanothece

------ Cyanothece aeruginosa

------ Derepyxis

------ Desmidium

------ Dinobryon

------ Euglena

- 1

---- Euglena polymorpha

------ Euglenophyceae

------ Geitlerinema

------ Geitlerinema acutissimum

------ Glenodinium

------As Entered / STORET station -> GAD104US LIB104LV CLA243LV CLA254LR UNI234LV LEV502GS Sample date -> 11/14/2005 11/21/2005 11/14/2005 11/14/2005 11/15/2005 12/6/2005

Taxon Name / SampleID/PerID -> Sampleid 49652/PerID

18430 (# counted) Sampleid 49677/PerID

18448 (# counted) Sampleid 52996/PerID

19568 (# counted) Sampleid 50131/PerID

19608 (# counted) Sampleid 53059/PerID

19639 (# counted) Sampleid 52471/PerID

20006 (# counted) Gomontiella

------ Hapalosiphon

------ Hapalosiphon hibernicus -----3 Heteroleibleinia

------ Heteroleibleinia kuetzingii

------ Jaaginema - 19 -

- 5 1 Komvophoron schmidlei

------ Lepocinclis

------ Leptolyngbya

- - - 3

- - Lyngbya martensiana

------ Merismopedia glauca 1

----- Merismopedia warmingiana

------ Micrasterias

------ Microcystis

------ Microspora

------ Microthamnion kuetzingianum

- 3

---- Mougeotia

- - 3

- - - Oedogonium

- - 1

- - 1 Oocystis

------ Ophiocytium capitatum

------ Oscillatoria

- - - 13 -

- Oscillatoria acutissima

------ Oscillatoria limosa

------ Oscillatoria princeps

------ Oscillatoria tenuis

------ Phacus

------ Phacus megalopsis

------ Phormidium

------ Phormidium aerugineo-caeruleum

------ Planktolyngbya 8737-2 Planktosphaeria

------ Planktothrix

- 2

- 1

- 3 Pleurocapsa -----1 Protococcus

------ Pseudanabaena 8 2 5 12 2 1 Pseudanabaena mucicola

------ Rhabdogloea

------ Scenedesmus

- - 1

- - - Scenedesmus abundans

------ Scenedesmus acuminatus

------ Scenedesmus bicaudatus

------ Scenedesmus bijuga

------ Scenedesmus dimorphus 1

----- Scenedesmus quadricauda 7

- - 1

- - Schizothrix calcicola 3

- - 14 - 13 Scytonema

------ Selenastrum

- 2

---- Spirogyra

- - 4

- - -As Entered / STORET station -> GAD104US LIB104LV CLA243LV CLA254LR UNI234LV LEV502GS Sample date -> 11/14/2005 11/21/2005 11/14/2005 11/14/2005 11/15/2005 12/6/2005

Taxon Name / SampleID/PerID -> Sampleid 49652/PerID

18430 (# counted) Sampleid 49677/PerID

18448 (# counted) Sampleid 52996/PerID

19568 (# counted) Sampleid 50131/PerID

19608 (# counted) Sampleid 53059/PerID

19639 (# counted) Sampleid 52471/PerID

20006 (# counted) Staurastrum 4

----- Staurastrum illusum illusum

------ Staurastrum lapponicum lapponicum

------ Stigeoclonium

- - 1

- - - Synechococcus

------ Synechocystis - 14

---- Tetraedron minimum

------ Tetraedron trigonum 3

----- Trachelomonas

------ Ulothrix

- - - 2

- -As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium PUT308GS GLA630GS BAK210GS BAK210GS 27311650820 8152 LEV502GS 12/13/2005 1/23/2006 11/1/2005 3/20/2006 6/21/2006 2/21/2007 Sampleid 52912/PerID

20031 (# counted) Sampleid 53951/PerID

20328 (# counted) Sampleid 47698/PerID

20630 (# counted) Sampleid 53732/PerID

20850 (# counted) Sampleid 52834/PerID

21128 (# counted) Sampleid 57322/PerID

21789 (# counted) 1-----

------

------

------ --1---

------ --1--- 286 186 247 261 175 251

------

------

------ -2---- 276331

------ -12- - -

------ -1---- --6---

------

------

------ -21- - -

------ -4---- 1-----

------

------

------

------

------ 1----- --1--- -1----

------ 33537- - - -67-

------

------

------

------

------

------ -2--3-

------ --1--- --1---

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra PUT308GS GLA630GS BAK210GS BAK210GS 27311650820 8152 LEV502GS 12/13/2005 1/23/2006 11/1/2005 3/20/2006 6/21/2006 2/21/2007 Sampleid 52912/PerID

20031 (# counted) Sampleid 53951/PerID

20328 (# counted) Sampleid 47698/PerID

20630 (# counted) Sampleid 53732/PerID

20850 (# counted) Sampleid 52834/PerID

21128 (# counted) Sampleid 57322/PerID

21789 (# counted)

------

------

------ ----9-

------ 1 21 6 7 51 5 -1---- -5----

------

------

------ -2---- --1--- ---1--

------

------ -4----

------

------

------ ----1-

------

------

------ -----3 -1----

------

------

------ 12- -5-

------ --6-1-

------

------ 3 1 5- - - -

------ -2---- -2--1-

------

------

------ ----1- -2---- -1--1-

- 3

- - 13 40 ---1-- - - -12- -3----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix PUT308GS GLA630GS BAK210GS BAK210GS 27311650820 8152 LEV502GS 12/13/2005 1/23/2006 11/1/2005 3/20/2006 6/21/2006 2/21/2007 Sampleid 52912/PerID

20031 (# counted) Sampleid 53951/PerID

20328 (# counted) Sampleid 47698/PerID

20630 (# counted) Sampleid 53732/PerID

20850 (# counted) Sampleid 52834/PerID

21128 (# counted) Sampleid 57322/PerID

21789 (# counted)

------

------

------

------

------ 1 17 9 12 15 - -1----

------ -41- - -

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium 3513 PUT308GS NUTREF001 LIB104LV 23010464 CLA246GS 2/20/2007 2/26/2007 6/10/2008 6/10/2008 5/7/2008 5/28/2008 Sampleid 57324/PerID

21790 (# counted) Sampleid 57431/PerID

21831 (# counted) Sampleid 65029/PerID

24392 (# counted) Sampleid 65025/PerID

24393 (# counted) Sampleid 64546/PerID

24394 (# counted) Sampleid 65250/PerID

24411 (# counted)

------

------

------

------

------ -1---1 -1---1 274 260 255 297 217 290

------

------

------ -2---1 -5- -33

------ 1-----

------

------

------

------ 2-----

------

------

------

------

------

------

------

------

------

------ -21- - -

------ -----2

------ 31- - - -

------

------

------

------

------

------

------ -1---1 ----1-

------

------

------ -----1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra 3513 PUT308GS NUTREF001 LIB104LV 23010464 CLA246GS 2/20/2007 2/26/2007 6/10/2008 6/10/2008 5/7/2008 5/28/2008 Sampleid 57324/PerID

21790 (# counted) Sampleid 57431/PerID

21831 (# counted) Sampleid 65029/PerID

24392 (# counted) Sampleid 65025/PerID

24393 (# counted) Sampleid 64546/PerID

24394 (# counted) Sampleid 65250/PerID

24411 (# counted)

------

------

------

------ - -1 1- - - 4 9 1 31 7 - 1---2-

------

------ 1-----

------

------

------ 1-----

------ -3----

------ 1-----

------

------

------ -1---- 1-----

------ 7-----

------

------

------

------ 3----- -1---- -3--3-

------

------ 3---4-

------

------

------

------

------

------

------

------

------ - -3 2-4 8 -

------ -2----

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix 3513 PUT308GS NUTREF001 LIB104LV 23010464 CLA246GS 2/20/2007 2/26/2007 6/10/2008 6/10/2008 5/7/2008 5/28/2008 Sampleid 57324/PerID

21790 (# counted) Sampleid 57431/PerID

21831 (# counted) Sampleid 65029/PerID

24392 (# counted) Sampleid 65025/PerID

24393 (# counted) Sampleid 64546/PerID

24394 (# counted) Sampleid 65250/PerID

24411 (# counted) -1----

------

------

------

------ -7--6-

------

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium 22020093 GAD106GS BAK208GS 3545 3546 3549 6/13/2008 6/13/2008 6/18/2008 7/9/2008 7/9/2008 7/10/2008 Sampleid 65026/PerID

24416 (# counted) Sampleid 65028/PerID

24417 (# counted) Sampleid 65306/PerID

24430 (# counted) Sampleid 65291/PerID

24441 (# counted) Sampleid 65296/PerID

24444 (# counted) Sampleid 65316/PerID

24445 (# counted)

------

------

------

------

------ ----1- ----1- 279 294 272 268 272 255

- - -2 0- -

------

------ - -42- - - -3233

------

------ 1-----

------

------

------

------ -----1

------ --3--- ---1--

------

------

------ ---1--

------

------ -----1

------

------

------ ----1-

------

------ -----1 ----2-

------ ---1--

------

------

------

------ -1----

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra 22020093 GAD106GS BAK208GS 3545 3546 3549 6/13/2008 6/13/2008 6/18/2008 7/9/2008 7/9/2008 7/10/2008 Sampleid 65026/PerID

24416 (# counted) Sampleid 65028/PerID

24417 (# counted) Sampleid 65306/PerID

24430 (# counted) Sampleid 65291/PerID

24441 (# counted) Sampleid 65296/PerID

24444 (# counted) Sampleid 65316/PerID

24445 (# counted) -1----

------

------

------

------ 4-124-

------

------

------ -----1

------

------

------

------

------

------ - -1 4- - -

------

------

------

------

------

------

------ -----6

------

------ -----8

------ 24-15-

------ 4- -11-

------ -----9 ----1-

------

------

------

------ ----1- 2----- 1-----

------ 2- - -15 1- - -26

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix 22020093 GAD106GS BAK208GS 3545 3546 3549 6/13/2008 6/13/2008 6/18/2008 7/9/2008 7/9/2008 7/10/2008 Sampleid 65026/PerID

24416 (# counted) Sampleid 65028/PerID

24417 (# counted) Sampleid 65306/PerID

24430 (# counted) Sampleid 65291/PerID

24441 (# counted) Sampleid 65296/PerID

24444 (# counted) Sampleid 65316/PerID

24445 (# counted)

------

------

------ --3---

------ 4- -375

------

------ ---1--

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium 32030023 21010008 WAK158LR 22050083 22050040 23010464 7/24/2008 7/16/2008 6/19/2008 8/12/2008 8/12/2008 10/8/2008 Sampleid 65292/PerID

24452 (# counted) Sampleid 65293/PerID

24458 (# counted) Sampleid 65414/PerID

24471 (# counted) Sampleid 65379/PerID

24479 (# counted) Sampleid 65382/PerID

24482 (# counted) Sampleid 65567/PerID

24491 (# counted)

------ 4----- - 14 0- - - - - -6 2- -

------

------ -2--1- 270 257 245 181 239 258

------

------ ---1--

------ 2137- - -----1

------ - - -141

------

------

------

------ --1---

------

------ --2--1

------ -----1

------

------ -----1 3----- 211- - -

------

------

------ -----7

------

------

------

------

------

------

------ 1-----

------

------

------ ----3-

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra 32030023 21010008 WAK158LR 22050083 22050040 23010464 7/24/2008 7/16/2008 6/19/2008 8/12/2008 8/12/2008 10/8/2008 Sampleid 65292/PerID

24452 (# counted) Sampleid 65293/PerID

24458 (# counted) Sampleid 65414/PerID

24471 (# counted) Sampleid 65379/PerID

24479 (# counted) Sampleid 65382/PerID

24482 (# counted) Sampleid 65567/PerID

24491 (# counted)

------

------

------

------

------ 3 13 - 13 11 12 -2---1 1----2

------

------

------

------

------

------

------ 2----- 2-4--- 1- -16- ---2--

------ -----1

------ ----3- ----9- 11- -3-

------ -----1 -----3 ----3- 13- -8-

------ 11-42-

------

------ -2-4-3

------

------

------

------

------

------ --1--1 -3---3 -1--1- - - -2 34 7

------

------ -1----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix 32030023 21010008 WAK158LR 22050083 22050040 23010464 7/24/2008 7/16/2008 6/19/2008 8/12/2008 8/12/2008 10/8/2008 Sampleid 65292/PerID

24452 (# counted) Sampleid 65293/PerID

24458 (# counted) Sampleid 65414/PerID

24471 (# counted) Sampleid 65379/PerID

24479 (# counted) Sampleid 65382/PerID

24482 (# counted) Sampleid 65567/PerID

24491 (# counted)

------

------ 1----- ----2-

------ 5 1 63 2 1 1

------

------ 11- - - - -2----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium UNI234LV CLA246GS WAK158LR 19010006 CLA254LR CLA243LV 10/16/2008 10/28/2008 11/13/2008 11/12/2008 11/6/2008 11/6/2008 Sampleid 65637/PerID

24513 (# counted) Sampleid 65636/PerID

24518 (# counted) Sampleid 65715/PerID

24541 (# counted) Sampleid 65731/PerID

24544 (# counted) Sampleid 65736/PerID

24548 (# counted) Sampleid 65737/PerID

24549 (# counted)

------

------ --9---

------

------

------ -1-1-- 68 282 225 280 284 294

------ -----1

------ ---2-- -351-2

------ ----2- ---3--

------

------ --1---

------

------

------

------

------

------

------ --1---

------

------ --2-1- -----1

------

------

------ --1---

------

------

------

------

------

------

------

------

------

------

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra UNI234LV CLA246GS WAK158LR 19010006 CLA254LR CLA243LV 10/16/2008 10/28/2008 11/13/2008 11/12/2008 11/6/2008 11/6/2008 Sampleid 65637/PerID

24513 (# counted) Sampleid 65636/PerID

24518 (# counted) Sampleid 65715/PerID

24541 (# counted) Sampleid 65731/PerID

24544 (# counted) Sampleid 65736/PerID

24548 (# counted) Sampleid 65737/PerID

24549 (# counted)

------

------

------

------

------ 1 41 63 3 1 --2---

------

------

------

------

------

------

------

------

------ -41- - - 2-----

------ -----1

------

------

------

------

------ -2-1--

------

------

------ -39- - -

------

------

------

------ --4---

------

------ --1--- ---2--

------

------ ---2--

------ ---2--

- - 20 - 9

-

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix UNI234LV CLA246GS WAK158LR 19010006 CLA254LR CLA243LV 10/16/2008 10/28/2008 11/13/2008 11/12/2008 11/6/2008 11/6/2008 Sampleid 65637/PerID

24513 (# counted) Sampleid 65636/PerID

24518 (# counted) Sampleid 65715/PerID

24541 (# counted) Sampleid 65731/PerID

24544 (# counted) Sampleid 65736/PerID

24548 (# counted) Sampleid 65737/PerID

24549 (# counted) --1--- ----1-

------ -1----

------ - -73- -

------

------

------ --3---As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium NUTREF001 GAD106GS GLA630GS 24010002 27311650820 8152 22050083 11/20/2008 11/20/2008 12/16/2008 12/17/2008 12/17/2008 1/14/2009 Sampleid 65739/PerID

24551 (# counted) Sampleid 65741/PerID

24553 (# counted) Sampleid 65891/PerID

24591 (# counted) Sampleid 65900/PerID

24599 (# counted) Sampleid 65907/PerID

24600 (# counted) Sampleid 65931/PerID

24605 (# counted)

------

------ 1-----

------

------

------ -----5 171 264 274 270 228 201

------

------ -----2 33- - -2 2 1 - - -2 2

------ -----5

------

------

------

------

------

------

------

------ -1----

------

------

------

------

------

------

------

------ -----1 -----1 -4---1

------ -----1

------ ----1- -----1

------ -----2

------

------

------

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra NUTREF001 GAD106GS GLA630GS 24010002 27311650820 8152 22050083 11/20/2008 11/20/2008 12/16/2008 12/17/2008 12/17/2008 1/14/2009 Sampleid 65739/PerID

24551 (# counted) Sampleid 65741/PerID

24553 (# counted) Sampleid 65891/PerID

24591 (# counted) Sampleid 65900/PerID

24599 (# counted) Sampleid 65907/PerID

24600 (# counted) Sampleid 65931/PerID

24605 (# counted)

------ --1---

------

------

------ 4 9 31 53 1

------

------

------

------

------

------

------

------ -----9

------

------ -----1

------

------

------

------

------

------ ---8--

------

------ ---1--

------ 113-49

------ - -11- - -1----

------ 46- - -1 1-----

------

------

------

------

------

------

------ ----1- 7 31 836 3 7

------

------

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix NUTREF001 GAD106GS GLA630GS 24010002 27311650820 8152 22050083 11/20/2008 11/20/2008 12/16/2008 12/17/2008 12/17/2008 1/14/2009 Sampleid 65739/PerID

24551 (# counted) Sampleid 65741/PerID

24553 (# counted) Sampleid 65891/PerID

24591 (# counted) Sampleid 65900/PerID

24599 (# counted) Sampleid 65907/PerID

24600 (# counted) Sampleid 65931/PerID

24605 (# counted) -1----

------

------ -----5 1----- - 6 - - -2 1

------

------ ---2--

------As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium 22050040 1/14/2009 Sampleid 65932/PerID

24606 (# counted) Phylum Class Order - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Cyanophycota Cyanophyceae Nostocales - Cyanophycota Cyanophyceae Nostocales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales 4 Cyanophycota Cyanophyceae Chroococcales 220 Bacillariophyta Bacillariophyta Bacillariophyta

- Rhodophycota Rhodophyceae Nemaliales - Xanthophyta Xanthophyceae Mischococcales - Cyanophycota Cyanophyceae Nostocales - Chlorophycota Chlorophyceae Volvocales 15 Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales 3 Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Volvocales 1 Chlorophycota Chlorophyceae Chlorophyceae - Cyanophycota Cyanophyceae Chroococcales 1 Cyanophycota Cyanophyceae Chroococcales - Cyanophycota Cyanophyceae Chroococcales - Chrysophyta Chrysophyceae Chrysophyceae - Chlorophycota Chlorophyceae Cladophorales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Chlorococcales - Cryptophycophyta Cryptophyceae Cryptomonadales - Cyanophycota Cyanophyceae Chroococcales 8 Cyanophycota Cyanophyceae Chroococcales - Cyanophycota Cyanophyceae Chroococcales - Cyanophycota Cyanophyceae Cyanophyceae - Cyanophycota Cyanophyceae Chroococcales - Cyanophycota Cyanophyceae Chroococcales - Haptophyta Prymnesiophyceae Isochrysidales - Chlorophycota Chlorophyceae Zygnematales - Chrysophyta Chrysophyceae Ochromonadales - Euglenophycota Euglenophyceae Euglenales - Euglenophycota Euglenophyceae Euglenales - Euglenophycota Euglenophyceae Euglenophyceae - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Pyrrophycophyta Dinophyceae PeridinialesAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra 22050040 1/14/2009 Sampleid 65932/PerID

24606 (# counted) Phylum Class Order - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Stigonematales - Cyanophycota Cyanophyceae Stigonematales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Euglenophycota Euglenophyceae Euglenales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Chroococcales - Cyanophycota Cyanophyceae Chroococcales - Chlorophycota Chlorophyceae Zygnematales - Cyanophycota Cyanophyceae Chroococcales - Chlorophycota Chlorophyceae Microsporales 1 Chlorophycota Chlorophyceae Chaetophorales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Oedogoniales - Chlorophycota Chlorophyceae Chlorococcales - Xanthophyta Xanthophyceae Mischococcales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Euglenophycota Euglenophyceae Euglenales - Euglenophycota Euglenophyceae Euglenales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales 16 Cyanophycota Cyanophyceae Oscillatoriales - Chlorophycota Chlorophyceae Chlorococcales 2 Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Pleurocapsales - Chlorophycota Chlorophyceae Ctenocladales 1 Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Chroococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales 7 Cyanophycota Cyanophyceae Oscillatoriales - Cyanophycota Cyanophyceae Nostocales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae ZygnematalesAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix 22050040 1/14/2009 Sampleid 65932/PerID

24606 (# counted) Phylum Class Order - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales - Chlorophycota Chlorophyceae Zygnematales 1 Chlorophycota Chlorophyceae Chaetophorales - Cyanophycota Cyanophyceae Chroococcales 19 Cyanophycota Cyanophyceae Chroococcales - Chlorophycota Chlorophyceae Chlorococcales - Chlorophycota Chlorophyceae Chlorococcales 1 Euglenophycota Euglenophyceae Euglenales - Chlorophycota Chlorophyceae UlotrichalesAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> A ctinotaenium A ctinotaenium perminutum A nabaena A nabaena subcylindrica A nkistrodesmus A nkistrodesmus falcatus A phanocapsa Bacillariophyta Batrachospermum Bumilleriopsis brevis Calothrix Chlamydomonas Chlorella Chlorella vulgaris Chlorococcum Chlorococcum humicola Chlorogonium Chlorophyceae Chroococcus Chroococcus minutus Chroococcus turgidus Chrysophyceae Cladophora Closterium Closterium gracile gracile Closterium lanceolatum lanceolatum Closterium moniliferum moniliferum Closterium parvulum obtusum Closterium peracerosum Closterium venus venus Cosmarium Crucigenia tetrapedia Cryptomonas Cyanobium Cyanobium parvum Cyanobium plancticum Cyanophyceae Cyanothece Cyanothece aeruginosa Derepyxis Desmidium Dinobryon Euglena Euglena polymorpha Euglenophyceae Geitlerinema Geitlerinema acutissimum Glenodinium Family Genus Current Name Desmidiaceae A ctinotaenium Desmidiaceae A ctinotaenium Nostocaceae A nabaena Nostocaceae A nabaena Oocystaceae A nkistrodesmus Oocystaceae A nkistrodesmus Merismopediaceae A phanocapsa Bacillariophyta Bacillariophyta Batrachospermaceae Batrachospermum Centritractaceae Bumilleriopsis Rivulariaceae Calothrix Chlamydomonadaceae Chlamydomonas Oocystaceae Chlorella Oocystaceae Chlorella Chlorococcaceae Chlorococcum Chlorococcaceae Chlorococcum Chlamydomonadaceae Chlorogonium Chlorophyceae Chlorophyceae Chroococcaceae Chroococcus Chroococcaceae Chroococcus Chroococcaceae Chroococcus Chrysophyceae Chrysophyceae Cladophoraceae Cladophora Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Closterium Desmidiaceae Cosmarium Scenedesmaceae Crucigenia Cryptomonadaceae Cryptomonas Synechococcaceae Cyanobium Synechococcaceae Cyanobium Synechococcaceae Cyanobium Cyanophyceae Cyanophyceae Synechococcaceae Cyanothece Synechococcaceae Cyanothece Derepyxidaceae Derepyxis Desmidiaceae Desmidium Dinobryaceae Dinobryon Euglenaceae Euglena Euglenaceae Euglena Euglenophyceae Euglenophyceae Pseudanabaenaceae Geitlerinema Pseudanabaenaceae Geitlerinema Glenodiniaceae GlenodiniumAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Gomontiella Hapalosiphon Hapalosiphon hibernicus Heteroleibleinia Heteroleibleinia kuetzingii Jaaginema Komvophoron schmidlei Lepocinclis Leptolyngbya Lyngbya martensiana Merismopedia glauca Merismopedia warmingiana Micrasterias Microcystis Microspora Microthamnion kuetzingianum Mougeotia Oedogonium Oocystis Ophiocytium capitatum Oscillatoria Oscillatoria acutissima Oscillatoria limosa Oscillatoria princeps Oscillatoria tenuis Phacus Phacus megalopsis Phormidium Phormidium aerugineo-caeruleum Planktolyngbya Planktosphaeria Planktothrix Pleurocapsa Protococcus Pseudanabaena Pseudanabaena mucicola Rhabdogloea Scenedesmus Scenedesmus abundans Scenedesmus acuminatus Scenedesmus bicaudatus Scenedesmus bijuga Scenedesmus dimorphus Scenedesmus quadricauda Schizothrix calcicola Scytonema Selenastrum Spirogyra Family Genus Current Name Gomontiellaceae Gomontiella Stigonemataceae Hapalosiphon Stigonemataceae Hapalosiphon Pseudanabaenaceae Heteroleibleinia Pseudanabaenaceae Heteroleibleinia Pseudanabaenaceae Jaaginema Borziaceae Komvophoron Euglenaceae Lepocinclis Pseudanabaenaceae Leptolyngbya Oscillatoriaceae Lyngbya Merismopediaceae Merismopedia Merismopediaceae Merismopedia Desmidiaceae Micrasterias Microcystaceae Microcystis Microsporaceae Microspora Chaetophoraceae Microthamnion Zygnemataceae Mougeotia Oedogoniaceae Oedogonium Oocystaceae Oocystis Ophiocytaceae Ophiocytium Oscillatoriaceae Oscillatoria Pseudanabaenaceae Geitlerinema Geitlerinema acutissimum Oscillatoriaceae Oscillatoria Oscillatoriaceae Oscillatoria Oscillatoriaceae Oscillatoria Euglenaceae Phacus Euglenaceae Phacus Phormidiaceae Phormidium Phormidiaceae Phormidium Pseudanabaenaceae Planktolyngbya Oocystaceae Planktosphaeria Phormidiaceae Planktothrix Pleurocapsaceae Pleurocapsa Protococcaceae Protococcus Pseudanabaenaceae Pseudanabaena Pseudanabaenaceae Pseudanabaena Synechococcaceae Rhabdogloea Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Scenedesmaceae Scenedesmus Oscillatoriaceae Schizothrix Scytonemataceae Scytonema Scenedesmaceae Selenastrum Zygnemataceae SpirogyraAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerID -> Staurastrum Staurastrum illusum illusum Staurastrum lapponicum lapponicum Stigeoclonium Synechococcus Synechocystis Tetraedron minimum Tetraedron trigonum Trachelomonas Ulothrix Family Genus Current Name Desmidiaceae Staurastrum Desmidiaceae Staurastrum Desmidiaceae Staurastrum Chaetophoraceae Stigeoclonium Chroococcaceae Synechococcus Chroococcaceae Synechocystis Chlorococcaceae Tetraedron Chlorococcaceae Tetraedron Euglenaceae Trachelomonas Ulotrichaceae UlothrixAs Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) A chnanthaceae

- --- A chnanthales

- --- A chnanthes

- 7 7 0 1 2 A chnanthes clevei 14 2 3 1 - A chnanthes exigua 24 135 A chnanthes fluviatilis

- --- A chnanthes hungarica

- --- A chnanthes lanceolata

- 374 A chnanthes lanceolata apiculata 68 3-9 A chnanthes lanceolata frequentissima 13

--- A chnanthes lanceolata rostrata 2 4-- A chnanthes minutissima

- -1- A chnanthes peragalli

- --- A chnanthes rupestoides

- 91 4

- A chnanthidiaceae

- --- A chnanthidium

- --- A chnanthidium exiguum

- --- A chnanthidium microcephalum

- --- A chnanthidium minutissimum

- --- A ctinella

- --- A mphipleura pellucida

- 2-- A mphora 4 17 6

- A mphora copulata

- --- A mphora libyca

- 2-- A mphora veneta

- 1-- A northoneis

- --- A

sterionella

- --- A ulacoseira 4 --3 A ulacoseira granulata

- --- A ulacoseira herzogii

- --- Bacillaria paxillife r 12

--- Bacillariophyceae

- 19 28 11 Bacillariophyta

- --- Brachysira

- --- Brachysira brebissonii

- --- Brachysira microcephala

- --- Brachysira vitrea 3

--- Caloneis

- --- Caloneis bacillum

- --- Capartogramma crucicula 11

--- Cavinula

- --- Cavinula cocconeiformis

- --- Cavinula jaernefeltii

- --- Chamaepinnularia margaritiana -

--- Chamaepinnularia minutissima

- --- Cocconeis

- 54- Cocconeis disculus

- --- Cocconeis fluviatilis 37 26

- -As Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) Cocconeis pediculus

- --- Cocconeis placentula

- 71 46

- Cocconeis pseudothumensis

- --- Coscinodiscophyceae

- --- Craticula 1 --2 Craticula minusculoides

- --- Cyclostephanos invisitatus

- --- Cyclotella

- --- Cyclotella atomus

- --- Cyclotella meneghiniana 2

--- Cyclotella pseudostelligera

- --- Cymbella

- -3- Cymbella aspera

- --- Cymbella subcuspidata

- --- Cymbellaceae

- --- Cymbellales

- --- Diadesmis confervacea 33 4 7 1 1 0 Diadesmis contenta

- 1-- Diatoma

- --- Diploneis 5

--- Diploneis oblongella

- --- Diploneis ovalis

- --- Diploneis parma

- --- Diploneis pseudovalis

- 1-- Encyonema

- --- Encyonema evergladianum

- 1-- Encyonema minutum

- --- Encyonema neomesianum

- --- Encyonema silesiacum

- -19 Encyonopsis microcephala

- --- Epithemia

- --- Eunotia 19 17

- 235 Eunotia bilunaris

- 2-1 Eunotia carolina

- --- Eunotia exigua

- --- Eunotia flexuosa

- --- Eunotia formica 1

--- Eunotia implicata

- --- Eunotia incisa

- --- Eunotia meisteri

- --- Eunotia minor

- --- Eunotia naegelii

- --- Eunotia paludosa

- --- Eunotia pectinalis

- --6 Eunotia pectinalis undulata

- --- Eunotia rhomboidea

- --- Eunotia transfuga

- --- Eunotia undulata

- ---As Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) Eunotia yanomami

- --- Eunotia zygodon

- --- Fragilaria

- 26- Fragilaria brevistriata 15

--- Fragilaria capucina

- --- Fragilaria crotonensis

- --- Fragilaria javanica

- --- Fragilaria pinnata 3 51- Fragilaria synegrotesca

- --- Fragilaria virescens

- --- Fragilariaceae 109 14 6

- Fragilariforma

- --- Fragilariforma bicapitata

- --- Fragilariforma virescens

- --- Fragilariophyceae

- -12 Frustulia

- --8 Frustulia rhomboides

- --- Frustulia saxonica

- --2 Frustulia vulgaris 1

--- Geissleria decussis

- --- Gomphonema 2 60 5 22 Gomphonema affine

- --- Gomphonema augur

- --- Gomphonema clevei

- --- Gomphonema gracile 1 --1 Gomphonema grovei lingulatum -

--- Gomphonema guaraniarum

- --- Gomphonema minutum

- --- Gomphonema parvulum

- --7 Gomphonema truncatum

- --- Gomphonema vibrio

- --- Gyrosigma 2

--- Gyrosigma obscurum

- -1- Hantzschia 2

--- Hantzschia amphioxys

- --- Hippodonta

- 8-- Hippodonta capitata 17 1-1 Hippodonta hungarica

- 2-1 Karayevia clevei

- --- Kobayasiella

- --- Lemnicola hungarica

- --- Luticola

- --- Luticola mutica

- --- Melosira 1

- 152

- Meridion

- --- Microcostatus polonarum

- --- Navicula 4 38 17 4 Navicula bouillantensis

- ---As Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) Navicula clementis

- --- Navicula cocconeiformis

- --3 Navicula constans 4 231 Navicula cryptocephala 6 7-4 Navicula cryptotenella

- -1- Navicula decussis

- --- Navicula elginensis 5

--- Navicula festiva

- --- Navicula gastrum 5

--- Navicula germainii 8

--- Navicula heimansioides

- --- Navicula ingenua

- --- Navicula kotschyi

- --- Navicula longicephala

- --- Navicula mediocris

- --- Navicula minima

- 211 Navicula occulta

- --- Navicula peregrina 11

--- Navicula placenta

- --- Navicula placentula

- --- Navicula porifera

- --- Navicula radiosa 6 1-- Navicula rhynchocephala

- --- Navicula rostellata 1

--- Navicula schroeteri

- --- Navicula seminulum 3 - -2 5 Navicula tripunctata

- --- Navicula vaneeii

- --- Navicula viridulacalcis

- 3-- Naviculaceae 18 5 1 9 3 0 Naviculales

- --- Neidium

- 2-- Neidium alpinum

- --- Neidium alpinum quadripunctatum -

--- Neidium amphigomphus

- --- Neidium binodis

- --- Neidium densestriatum

- --1 Neidium dubium

- --- Neidium ladogensis densestriatum -

--- Nitzschia 1 23 9 31 Nitzschia amphibia

- --- Nitzschia clausii

- --- Nitzschia dissipata

- --- Nitzschia frustulum

- --- Nitzschia gracilis 1

--- Nitzschia intermedia

- --- Nitzschia linearis

- --- Nitzschia nana

- --2As Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) Nitzschia palea 4

--- Nitzschia paleacea

- --- Nitzschia palustris

- --- Nitzschia reversa

- --- Nitzschia scalaris

- --- Nitzschia seriata

- --- Nitzschia serrata

- --- Nitzschia subacicularis

- --- Nupela

- --- Nupela cymbelloidea

- --- Nupela neotropica

- --- Opephora

- --- Pinnularia

- 6 -4 3 Pinnularia acrosphaeria

- --- Placoneis

- --- Placoneis elginensis

- --- Planothidium

- --- Planothidium apiculatum

- --- Planothidium delicatulum

- --- Planothidium frequentissimum

- --- Planothidium lanceolatum

- --- Planothidium peragalli

- --- Planothidium rostratum

- --- Pleurosira laevis

- --- Psammothidium daonense

- --- Psammothidium sacculum

- --- Pseudostaurosira brevistriata

- --- Rhopalodia

- --- Rossithidium

- --- Rossithidium pusillium

- --- Sellaphora

- 2-- Sellaphora laevissima

- --- Sellaphora pupula 8 --2 Stauroneis

- 1-- Stauroneis anceps

- --- Stauroneis phoenicenteron

- --- Stauroneis prominula

- --- Stauroneis smithii 6

--- Staurosira construens

- --- Staurosira elliptica

- --- Staurosirella

- --- Staurosirella leptostauron

- --- Staurosirella pinnata

- --- Stenopterobia

- --- Stenopterobia delicatissima

- --- Stephanodiscus

- --- Surirella

- --2 Surirella angusta

- ---As Entered / STORET station -> 273116508208152 LEV502GS 3513 PUT308GS Sample date -> 6/21/2006 2/21/2007 2/20/2007 2/26/2007

Taxon Name / SampleID/PerDiaID -> Sampleid 52834/PerDiaID 21128 (# counted) Sampleid 57322/PerDiaID 21789 (# counted) Sampleid 57324/PerDiaID 21790 (# counted) Sampleid 57431/PerDiaID 21831 (# counted) Synedra

- 11- Synedra acus

- --- Synedra fasciculata

- -2- Synedra ulna

- 36

- - Tabellaria

- --- Terpsinoe musica 3

--- Thalassionema

- --- Thalassiosira

- --- Tryblionella

- --- Tryblionella levidensis

- ---As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted)

---- 64 82

- - 75 45 12 9

---- 16 1 19

-

---- --3-

----

----

----

----

---- -1-- 2

3- - -

---- 46 41 2

- ---2 -24- - 1 4- -

---- 1--- -2--

----

----

----

----

----

- - 29

-

----

----

---- 32 42 16 22 1--- 10

- 4 2

----

---- - -71

---- 2---

----

----

----

----

----

----

- - 15

-

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted)

----

- - 63

-

---- 1---

----

---- --1- 3-7- --2- --2-

----

----

----

---- 2

- 12 6

---- --3-

----

----

----

----

----

----

---- 2-1-

---- - - 22 1 --1- ---3 --2-

---- 73 145 21 278 2---

----

----

---- ---1

---- - - -2 6

----

---- ---6 12- -

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted)

----

----

----

---- --2-

----

----

----

----

---- 21

- 4

-

---- - - -1 1

----

---- 5 2 -1 0

---- 24- -

----

---- 12 10 1

-

----

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---- 1

- 11

-

----

----

----

----

----

---- 1--- 1121

----

----

----

---- 2---

----

----

----

---- 13

- 18 9

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted)

----

----

---- 2-5- --4-

---- ---2

----

----

----

----

----

----

----

----

- - 74 52

----

----

----

----

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---- 11

- 2 4

---- ---1

----

----

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----

---- 27 12 103 9 2---

----

----

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---- --7-As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted) 2--- -4--

---- --1-

----

----

----

----

----

----

---- --3- 16 83 2 16

----

----

---- 13 3

- -

----

----

---- 12

- 15 1

---- --6-

----

----

----

---- 1--3

----

----

----

---- 2-5- -1--

----

----

----

----

----

----

----

----

---- ---3

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis NUTREF001 LIB104LV 23010464 CLA246GS 6/10/2008 6/10/2008 5/7/2008 5/28/2008

Sampleid 65029/PerDiaID 24392 (# counted) Sampleid 65025/PerDiaID 24393 (# counted) Sampleid 64546/PerDiaID 24394 (# counted) Sampleid 65250/PerDiaID 24411 (# counted) --2- --1-

---- - -11

----

---- --1-

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted) 8--- -4--

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

---- 16 1

- -

----

---- 4---

----

----

----

----

---- 3---

---- 2--- 2--- 14 15 5 7

----

---- ---3 ---4

----

---- -11- 3---

----

----

----

----

---- 4---

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted)

---- 6---

----

----

----

----

---- 4---

----

---- 12 1

- -

---- 1---

---- 4--- - - -1 1 1---

----

---- 1---

----

----

----

---- ---1

---- 6---

---- 1---

----

---- 20 103 260 280 -1-- ---1 - - 91 0

----

----

---- - 5 81 1

---- ---4 --1- -23-

---- ---4

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted) ---1

----

----

---- 6---

----

----

---- 1---

---- 7--5

----

---- ---8

----

- 4 72 24

----

- - 29

-

---- -1-- 2---

----

----

---- 1--4 1 0- - -

----

----

----

----

----

----

----

----

----

---- 2--2 2--- 1-2-

----

----

----

---- --1-

----

---- 144 265

- 17

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted)

----

----

---- 22

- - 12 4---

----

----

----

----

----

----

----

----

----

---- 108 3

- -

----

----

----

----

----

----

----

----

----

----

----

----

---- 25 63

- 17 - - -1 5 - 4 -4 0

----

----

----

---- ---2

----

---- 28 5 95 2 1---

----

----

---- ---2

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted) -15-

----

----

----

----

----

----

----

----

----

----

---- 2 1 39 1

----

----

----

----

----

----

---- 95- -

----

----

----

----

---- 1---

----

----

---- 1--- 1--- 63- - ---6

----

----

----

---- 2--- 1---

----

----

----

---- ---6

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis 22020093 GAD106GS BAK208GS 3545 6/13/2008 6/13/2008 6/18/2008 7/9/2008

Sampleid 65026/PerDiaID 24416 (# counted) Sampleid 65028/PerDiaID 24417 (# counted) Sampleid 65306/PerDiaID 24430 (# counted) Sampleid 65291/PerDiaID 24441 (# counted)

----

----

---- 1---

----

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

---- 2

3- - - - -26

---- - - -2 8 - - -3 5

----

----

----

----

----

----

---- 2--- 20 9

- - 54 45

- - 3 2 6- -

---- 43 46 1

- --9-

---- 1--5

----

----

---- ---4 --2- 13- -

----

---- -1-1 33 12

- -

---- 1--- -18- - 1 1- -

----

----

---- -1-1

----

----

----

----

---- 12 21

- 14 ---1 ---7As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

---- - - -2 3

----

---- - -71 ---9

----

----

---- -1-- 3--- 21 1

- -

----

---- -2-2

---- - - 27 5

---- 1 3- - -

----

----

----

---- ---1 --3-

---- ---2

---- 1--1

----

---- 1 11 322

- --4-

----

---- --3-

----

----

- - 11

-

----

----

----

----

----

---- --3-

---- --1-As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

----

----

----

---- 1114

----

----

----

----

---- 2

5- - - --1-

----

----

----

- 3 36

-

----

----

----

---- - 1 62 8

---- 3---

----

----

----

---- - 1 0- - ---5

----

---- -2-1

----

----

----

---- -1-1

---- 1---

----

---- - -31

- - 14

- ---9

----

---- 55 43 13 61

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

----

----

---- 10 1

- 4 1---

----

----

----

----

----

----

----

----

----

---- 19 12

- 63

---- ---3

----

----

----

----

----

----

---- - - -1 7

----

---- 12

- - 3 14 31 20 10

----

---- --4- --2-

----

---- --1-

----

---- 23 69 5 26 -8-- 1---

----

---- 1--- ---3

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

----

----

----

----

----

----

----

---- - - 21 5

----

---- 2--- - -21

----

----

---- 51 74

- 5 - -15 3--1 20 34

- 2

----

---- 21

- - 7 ---2

----

----

----

----

----

---- 3---

---- -1-- --9-

----

----

----

----

---- ---2 -2-- 1---

----

---- --4-

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis 3546 3549 32030023 21010008 7/9/2008 7/10/2008 7/24/2008 7/16/2008

Sampleid 65296/PerDiaID 24444 (# counted) Sampleid 65316/PerDiaID 24445 (# counted) Sampleid 65292/PerDiaID 24452 (# counted) Sampleid 65293/PerDiaID 24458 (# counted)

----

----

---- 1 1 -2 0 --2-

----

---- ---4

---- ---1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted)

----

---- -38-

----

---- - 1 3 2 7 -

----

----

----

----

----

----

----

- 46 195

- 966- 73 2 1

- 46 36 24 43

---- 12

- 2 1

----

---- 421-

----

----

----

----

---- 202

- - 8

----

----

---- 6--6

---- 2---

----

----

----

---- ---2 ---2

----

----

----

----

---- 62-1

---- ---1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted) -2--

- 118 21 15

---- 1---

----

----

---- ---3 ---2 7-15

----

----

----

---- ---2

---- 49 49 9 5

----

----

----

----

----

----

----

----

----

----

---- ---7

----

---- 11

- 11 8 --9-

----

----

---- ---2

----

----

----

---- ---2

----

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted)

----

----

----

---- ---1

----

----

----

----

---- -4-4

----

----

----

---- ---2

----

----

----

---- - 1 3 2 8 2 0 ---2

---- --6- -128

---- --1-

---- - 9 23 6 -1-1

---- -2--

----

----

---- -2-6 ---3 ---1 -14-

----

----

---- --1-

----

----

---- 1 4 0 2 0 2 9

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted) -1--

----

---- - -32 - 5 -2 9

---- - 1 -1 1

---- --2-

----

---- -14- -17- --1-

----

- 6 18 8

----

----

----

----

---- -1-3 -1--

----

---- - 1 3 1 0 2 2

----

---- --2- -44- 3--3

----

----

----

---- --1- 2---

----

---- 27 57 20 127 - 1 1 1 4 4

----

----

----

---- ---4

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted)

----

----

---- -1--

----

----

----

----

- 8 14 18

---- - - -1 0

---- 31- -

----

----

---- 7-11 - -16 1--- 8542

----

---- - 1 33 4

----

----

----

---- 21- - -1-- -84- -1--

---- -142 -531

----

----

---- - -12

---- 1 8- - -

----

----

----

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis WAK158LR 22050083 22050040 23010464 6/19/2008 8/12/2008 8/12/2008 10/8/2008

Sampleid 65414/PerDiaID 24471 (# counted) Sampleid 65379/PerDiaID 24479 (# counted) Sampleid 65382/PerDiaID 24482 (# counted) Sampleid 65567/PerDiaID 24491 (# counted)

----

----

---- - - -1 3

----

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted)

----

---- 2 2 5 1 6 1

----

---- 144-

----

----

----

----

----

----

----

---- -4-- 1

- 13

- 14 3 70 57

---- --9-

----

---- -14-

----

----

----

----

---- 78 60 103 16 3---

---- ---1 -2--

----

----

----

----

---- 12 2

- -

---- ---1 -1-- 42- -

----

- - 38

-

- - 10

- ---7

---- ---2As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted)

---- - - -6 1 -2-- 3--- ---5

---- 1--- 2--- 3--- 7--3 1---

----

----

----

----

---- 14

- 69 24 -2--

----

---- --1-

---- 1---

----

----

---- -2-1 --4- -51-

----

---- 64 87 1 12 73-2

----

---- -3-- 3---

---- -3--

----

---- -2-- 39- -

----

---- 21- -

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted)

----

----

----

---- -3--

----

----

----

----

---- 4 2 94 2 ---1 -1--

----

---- 4 1 9- 1

----

---- -3--

---- 32-3

----

----

----

----

---- 1---

---- 11- -

----

----

----

----

---- 1--- ---2

---- 34- -

---- 13-2 1--- 17 1

- - ---1 -1-- 1---

---- 18 11 6 3

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted) ---4

----

----

----

----

----

---- 3--- 1--2 -1--

----

---- 1--- 21- - -2-- -718 11- -

---- ---4 1---

----

----

----

----

---- 8 5 021 3

----

----

---- 2666

---- ---7 -6-- -1--

----

---- 3-17

----

---- 8 23 22 105

----

----

----

----

----

----

---- -1--As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted) - 9 -3 0

---- 1 3- - -

----

----

---- ---2 2--- 103 64 37 5

---- --8- -1-- 52 28 11 34

----

---- -1-- 1242 2 5 11 5

---- - -34

----

---- 2-5-

----

---- --4- --1- -35-

---- -821 3-1-

---- 31-1 2315

---- 32- -

----

----

---- - -91

----

---- -2-6

---- --4- 4--- --3-

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis UNI234LV CLA246GS WAK158LR 19010006 10/16/2008 10/28/2008 11/13/2008 11/12/2008

Sampleid 65637/PerDiaID 24513 (# counted) Sampleid 65636/PerDiaID 24518 (# counted) Sampleid 65715/PerDiaID 24541 (# counted) Sampleid 65731/PerDiaID 24544 (# counted)

----

----

----

----

----

----

---- --1- ---2

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted)

----

---- 11 4

- -

----

---- 8-21

----

----

----

----

----

----

---- 27

- 1 4

---- -169 5-93

---- 11-1 -231

---- --1-

----

----

----

----

---- 6-71

----

----

----

----

---- ---1

----

---- --1- 2231

----

----

---- - 1 03 1 27- -

---- ---5 1 3- - -

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted)

---- 9---

----

---- 7 1 15 18

----

----

---- --1-

---- - -21

----

---- --2-

----

---- 1--- - -72 -2--

----

----

---- --1-

---- - -11

---- 2---

---- 3--2

----

---- 118 264 64 105

-122

----

----

----

----

---- 22- - - -11

----

---- -331

----

---- ---1

---- 1--2As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted) -3--

----

----

----

----

----

----

----

----

---- 7--- -5-8

---- 1---

---- 95 11 7 22

----

---- 2

- 15 4 1--- 213-

----

----

----

----

----

----

---- 4-28

----

----

----

----

----

---- -2-- --1- 3-41 --1- -194

---- 2-1- 1-1-

----

----

- - 23 16 14 1 13 17

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted) -2--

----

---- 2-41 - -12

----

---- --1-

---- 1---

----

----

---- 2-2- -1-1 7 6 12

-

----

----

----

----

---- 1-13 --1-

----

---- 12 1 22 2

----

----

---- 27 3 19 10

---- - -25 ---1 - -32 1---

---- - 1 11 1

----

---- 4 3 8 4 3 5 5

----

---- 2-11

----

----

----

---- ---1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted) --2-

----

----

----

---- ---1 -3--

---- 36 67 52 46

---- -6--

---- 7 2 7 2 7 5 4

----

----

---- 12 1 45 10 1 71 3 3

---- - - -2 2

- - 11

- 2-8- 6---

---- - -67 - - 11 4

----

----

---- --5- ---2 ---1 - -33 2666 3--- ---2

---- -2--

----

----

----

---- 1-1-

---- 3---

---- 2-1-

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis CLA254LR CLA243LV NUTREF001 GAD106GS 11/6/2008 11/6/2008 11/20/2008 11/20/2008

Sampleid 65736/PerDiaID 24548 (# counted) Sampleid 65737/PerDiaID 24549 (# counted) Sampleid 65739/PerDiaID 24551 (# counted) Sampleid 65741/PerDiaID 24553 (# counted)

----

----

---- --1-

----

----

----

---- -1--

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted)

-- - -

-- - - 3- - -

-- - -

-- - - 3- - 7

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

- - - 177

-- - -

-- - -

- 4 24 10 -- - 1 -- 1 4

-- - - -- - 5

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - -- - 3

-- - -

-- - - -4 7 -

-- - -

-- - -

-- - -

-- - - -- - 2

-- - - 1- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - -3 4 2

-- - -

- - 34

-As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted)

-- - -

- 2 3 102

-- - -

-- - - 4- 2 -

-- - -

-- - - -1 - -

-- - - -- - 3

-- - -

-- - - -- - 1

-- - -

-- - -

-- - - 66 3 - 1- 1 1

-- - - -- 2 -

-- - -

-- - - -- 1 -

-- - - 1- - -

-- - - -- 1 - 5- - - 81

- - - -- - 1 -- - 4 181

- 6 1

-- 1 2

-- - -

-- - -

-- - - -- 1 -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - -- 1 -

-- - -

-- - -As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted)

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - - 2 2 9 8 2

-- - -

-- - -

-- - -

-- - - 6- - -

-- - -

-- - - 3- - 1 -2 - - 14 1 2 -- - 2

-- - -

-- - - 2- - 1

-- - -

-- - - -- - 2 -- - 4

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - -2 6 -

- 2 11

- 1 1 11

-

- 2 11 3 7- - 1 -- - 7

-- - -

-- - -

- 384 2

-

-- - -

-- - - 13 3 44 13 -- - 1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted) -- 1 -

-- - - 1- 1 - -- - 2 -- - 2

-- - -

-- - - 1- - - 1- 5 -

- 4 25

- -- - 4 -- - 1

-- - - -1 4 -

-- - - 1- 1 6

-- - - -5 - -

-- - -

-- - - -- 1 - -- 2 -

-- - -

-- - -

-- - - -- - 5 -- 6 - -1 - -

-- - - -- 5 4

-- - -

-- - -

-- - - 1- - -

-- - -

-- - -

-- - -

-- - -

-- - - 31 10 31 8 -- - 1 7 -- 1 - -- - 4

-- - -

-- - -

-- - -

-- - - 5- - -As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted)

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - -

-- - - 58

- 6 6 -- - 4

-- - - -3 4 - 4- 3 2

-- - -

-- - - 13 6 - 37 8 38 3 6 7 12

-

-- - - -2 6 5 20

- 2

-

-- - -

- 8 19 45

-- - -

-- - -

-- - - -- 9 - -- - 2

-- - - 9- - 4 -- 2 -

-- - - -- 5 2 4- - 1

-- - - 1- - 1 -- 5 -

-- - -

-- - -

-- - -

-- - -

-- - -

- 6 20

-

-- - -

-- - -

-- - -

-- - - -1 1 1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis GLA630GS 24010002 273116508208152 22050083 12/16/2008 12/17/2008 12/17/2008 1/14/2009

Sampleid 65891/PerDiaID 24591 (# counted) Sampleid 65900/PerDiaID 24599 (# counted) Sampleid 65907/PerDiaID 24600 (# counted) Sampleid 65931/PerDiaID 24605 (# counted)

-- - -

-- - -

-- - - -- - 6

-- - -

-- - -

-- - - -- 3 -

-- - -

-- - -As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

----

---- - -77

---- ---4 8---

---- - -11 ---4

----

---- - 2 24 33

---- 52

- - 3

---- 2--- 8---

----

----

----

---- 1--- 1---

----

----

----

---- 2-22

----

----

---- - 23 15 9

---- 14- -

----

----

---- 2--4

---- --1-

----

----

---- 2---

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

---- 24 2

- 2

----

----

----

----

---- ---6

---- -2--

----

---- 1---

---- - -22

---- 6-2- 12- -

----

----

----

----

----

---- - 1 11 3

----

---- ---1

----

----

---- 1 312 170 148 -3--

----

----

----

----

---- -3--

----

----

----

---- - 1 7- -

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

---- -2--

- - 18 1

---- ---2

---- -2--

----

----

----

- - 14 2

----

----

---- -3-- -2-- - 1 4 9 0 9 6 - 1 0- - 1---

---- 18 2

- 1

----

----

----

----

----

----

----

----

----

----

----

----

----

----

---- --1-

---- 1--- 2

9- - -

---- -1--

----

----

----

---- 55 2

- 5

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

---- --3-

---- 10 1

- 8 11

- - 1

---- -1--

----

----

----

---- 1 8- - -

----

----

---- 56 13

- 5

----

----

----

----

----

----

----

---- -1-- 70 6

- -

----

----

---- 6561

---- --2-

----

----

----

----

----

---- -3-- 9 9 69 41 3---

---- 4---

- - 17

-

----

---- 2---

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

- - 16 8

----

----

----

----

----

----

---- 2

8- - - 1 2- - -

----

---- - 5 0 4 3 7 1

---- 1---

---- 1 1- - -

----

---- 2--- 3---

---- 2

3- - -

----

---- 4---

----

----

---- 7---

----

---- 21- - 2116

----

----

---- ---1

----

----

----

----

----

---- - - -1 0

---- ---2

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis 22050040 LIB104LV CLA243LV CLA254LR 1/14/2009 11/21/2005 11/14/2005 11/14/2005

Sampleid 65932/PerDiaID 24606 (# counted) Sampleid 49678/PerID 18449 (# counted) Sampleid 52997/PerID 19569 (# counted) Sampleid 53033/PerID 19609 (# counted)

----

----

----

----

----

----

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted)

----

---- -3-2 --4- 24-1

---- - 4 -2 1 -1-- 1 - 12 0

---- 22- - -2--

----

- 155

- -

----

----

----

----

----

---- - 2 8- -

----

---- ---8 ---1

----

---- 34

- 2 7 --2-

---- - 1 2- - 7225

----

----

----

---- ---1

---- 2--1 1---

----

----

----

----

---- -1--

---- - 2 7- -As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted)

---- - 1 5- 1

----

---- ---7

----

----

----

---- ---1

----

----

----

---- - 1 8- -

---- 1139 --3-

----

----

---- -1-1

----

---- - -58

---- ---4

----

----

----

---- 143 7 296 109

1--4

----

----

---- 3--- 3---

- - 32 4

----

----

----

----

- - 16

-

----

----

----

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted)

----

---- -14-

----

----

----

---- -21-

---- --4- -22-

----

----

---- ---1 85- - --1- 20

- 36 16

---- - - -1 4 2 4 8- 8 -1--

----

---- -8-6

----

----

---- -4-8

---- -1-- - 2 0- -

---- 1---

---- -2-7 ---4

----

----

----

---- -1-2

---- -61-

----

---- 6 3 9 1 1 2 8

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted)

---- 1-2- 1--- - 2 3- 4

---- -1--

----

---- 1---

----

----

----

----

----

---- - 3 14 1

----

----

----

----

---- -3--

----

----

---- -113

----

----

---- 1 - 81 2

---- - -21

----

----

----

----

---- ---3 2--- 64 31 7 22

----

----

----

---- 1--- -3--

---- ---1As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted) 23

- 23 29

----

----

---- --2-

----

----

----

----

----

----

---- 155 9 27 27

2---

----

----

----

----

----

----

----

----

----

----

----

----

---- ---2

----

----

---- - - -1 3 - 1 -1 1 1 - -1 2 --1- ---4

---- -2-1

----

----

----

----

----

----

----

---- ---4

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis UNI234LV LEV502GS PUT308GS GLA630GS 11/15/2005 12/6/2005 12/13/2005 1/23/2006

Sampleid 53060/PerID 19640 (# counted) Sampleid 54263/PerID 20007 (# counted) Sampleid 54275/PerID 20032 (# counted) Sampleid 55073/PerID 20329 (# counted)

----

----

---- ---1

----

----

---- 2--- 8---

----As Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -29 Bacillariophyt a Bacillariophy c -2- Bacillariophyt a Bacillariophy c -1- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --2 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --2 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 1-7 Bacillariophyt a Bacillariophy c - -1 0 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -1- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Fragilariophy c 21 40 29 Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Coscinodisco p --3 Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Bacillariophy c 6 3 4 1 1 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophyt a

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c - 1 1 - Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --6 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --1 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c - -6 1 Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class

--- Bacillariophyt a Bacillariophy c -3- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Coscinodisco p -1- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -22 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 1 2 1 6 Bacillariophyt a Bacillariophy c -11 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --3 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 358 200 37 Bacillariophyt a Bacillariophy c -9- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -2- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 1 1 4 - Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -6- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 168 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c - 1 2 - Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class

--- Bacillariophyt a Bacillariophy c 6-- Bacillariophyt a Bacillariophy c --7 Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c --2 Bacillariophyt a Fragilariophy c -5- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c - 1 8 3 Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c 1-- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c 6 1 2 - Bacillariophyt a Bacillariophy c - -2 6 Bacillariophyt a Bacillariophy c 731 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 1 14 6 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --5 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 1 -2 2 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --3 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c - -1 1 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --3 Bacillariophyt a Bacillariophy c -9- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Bacillariophy c - -5 1 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --5 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --2 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --6 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c - -1 8 Bacillariophyt a Bacillariophy c - -2 0 Bacillariophyt a Bacillariophy c --5 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --1 Bacillariophyt a Bacillariophy c - 11 7 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -1- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 63- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c 30 5 23 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -1- Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class -8- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Fragilariophy c 53 61 10 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Fragilariophy c -12 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c -1- Bacillariophyt a Bacillariophy c --3 Bacillariophyt a Bacillariophy c -2- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c --1 Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis BAK210GS BAK210GS GAD104US 11/1/2005 3/20/2006 11/14/2005

Sampleid 55672/PerID 20631 (# counted) Sampleid 55852/PerID 20851 (# counted) Sampleid 57811/PerID 22009 (# counted) Phylum Class

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c --9 Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Fragilariophy c

--- Bacillariophyt a Coscinodisco p

--- Bacillariophyt a Bacillariophy c

--- Bacillariophyt a Bacillariophy cAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> A chnanthaceae A chnanthales A chnanthes A chnanthes clevei A chnanthes exigua A chnanthes fluviatilis A chnanthes hungarica A chnanthes lanceolata A chnanthes lanceolata apiculata A chnanthes lanceolata frequentissima A chnanthes lanceolata rostrata A chnanthes minutissima A chnanthes peragalli A chnanthes rupestoides A chnanthidiaceae A chnanthidium A chnanthidium exiguum A chnanthidium microcephalum A chnanthidium minutissimum A ctinella A mphipleura pellucida A mphora A mphora copulata A mphora libyca A mphora veneta A northoneis A

sterionella A ulacoseira A ulacoseira granulata A ulacoseira herzogii Bacillaria paxillife r Bacillariophyceae Bacillariophyta Brachysira Brachysira brebissonii Brachysira microcephala Brachysira vitrea Caloneis Caloneis bacillum Capartogramma crucicula Cavinula Cavinula cocconeiformis Cavinula jaernefeltii Chamaepinnularia margaritiana Chamaepinnularia minutissima Cocconeis Cocconeis disculus Cocconeis fluviatilis Order Family Genus Current Name A chnanthales A chnanthace a A chnanthaceae A chnanthales A chnanthales A chnanthales A chnanthales A chnanthace a A chnanthes A chnanthales A chnanthidia c Karayevia Karayevia clevei A chnanthales A chnanthace a A chnanthes A chnanthidium exiguum A chnanthales A chnanthace a A chnanthes A chnanthales A chnanthidia c Lemnicola Lemnicola hungarica A chnanthales A chnanthidia c Planothidium Planothidium lanceolatum A chnanthales A chnanthidia c Planothidium Planothidium apiculatum A chnanthales A chnanthidia c Planothidium Planothidium frequentissimum A chnanthales A chnanthidia c Planothidium Planothidium rostratum A chnanthales A chnanthidia c A chnanthidiu m A chnanthidium minutissimum A chnanthales A chnanthace a A chnanthes A chnanthales A chnanthace a A chnanthes A chnanthales A chnanthidia c A chnanthidiaceae A chnanthales A chnanthidia c A chnanthidium A chnanthales A chnanthace a A chnanthes A chnanthales A chnanthidia c A chnanthidium A chnanthales A chnanthidia c A chnanthidium Eunotiales Eunotiaceae A ctinella Naviculales A mphipleura c A mphipleura Thalassiophy s Catenulacea e A mphora Thalassiophy s Catenulacea e A mphora Thalassiophy s Catenulacea e A mphora A mphora copulata Thalassiophy s Catenulacea e A mphora A chnanthales Cocconeidac e

A northoneis Fragilariales Fragilariacea e A

sterionella A ulacoseirale s A ulacoseirac e

A ulacoseira A ulacoseirale s A ulacoseirac e

A ulacoseira A ulacoseirale s A ulacoseirac e

A ulacoseira Bacillariales Bacillariacea e Bacillaria Bacillariophy c Bacillariophy c Bacillariophyceae Bacillariophyt a Bacillariophyt a Bacillariophyta Naviculales Brachysirace a Brachysira Naviculales Brachysirace a Brachysira Naviculales Brachysirace a Brachysira Naviculales Brachysirace a Brachysira Naviculales Pinnulariacea Caloneis Naviculales Pinnulariacea Caloneis Naviculales Naviculaceae Capartogramma Naviculales Cavinulaceae Cavinula Naviculales Cavinulaceae Cavinula Naviculales Cavinulaceae Cavinula Naviculales Naviculaceae Chamaepinnularia Naviculales Naviculaceae Chamaepinnularia A chnanthales Cocconeidac e

Cocconeis A chnanthales Cocconeidac e

Cocconeis A chnanthales Cocconeidac e

CocconeisAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Cocconeis pediculus Cocconeis placentula Cocconeis pseudothumensis Coscinodiscophyceae Craticula Craticula minusculoides Cyclostephanos invisitatus Cyclotella Cyclotella atomus Cyclotella meneghiniana Cyclotella pseudostelligera Cymbella Cymbella aspera Cymbella subcuspidata Cymbellaceae Cymbellales Diadesmis confervacea Diadesmis contenta Diatoma Diploneis Diploneis oblongella Diploneis ovalis Diploneis parma Diploneis pseudovalis Encyonema Encyonema evergladianum Encyonema minutum Encyonema neomesianum Encyonema silesiacum Encyonopsis microcephala Epithemia Eunotia Eunotia bilunaris Eunotia carolina Eunotia exigua Eunotia flexuosa Eunotia formica Eunotia implicata Eunotia incisa Eunotia meisteri Eunotia minor Eunotia naegelii Eunotia paludosa Eunotia pectinalis Eunotia pectinalis undulata Eunotia rhomboidea Eunotia transfuga Eunotia undulata Order Family Genus Current Name A chnanthales Cocconeidac e

Cocconeis A chnanthales Cocconeidac e

Cocconeis A chnanthales Cocconeidac e

Cocconeis Coscinodisco p Coscinodisco p Coscinodiscophyceae Naviculales Stauroneidac e Craticula Naviculales Stauroneidac e Craticula Thalassiosiral Stephanodisc Cyclostephanos Thalassiosiral Stephanodisc Cyclotella Thalassiosiral Stephanodisc Cyclotella Thalassiosiral Stephanodisc Cyclotella Thalassiosiral Stephanodisc Cyclotella Cymbellales Cymbellacea e Cymbella Cymbellales Cymbellacea e Cymbella Cymbellales Cymbellacea e Cymbella Cymbellales Cymbellacea e Cymbellaceae Cymbellales Cymbellales Cymbellales Naviculales Diadesmidac e Diadesmis Naviculales Diadesmidac e Diadesmis Fragilariales Fragilariacea e Diatoma Naviculales Diploneidace a Diploneis Naviculales Diploneidace a Diploneis Naviculales Diploneidace a Diploneis Naviculales Diploneidace a Diploneis Naviculales Diploneidace a Diploneis Cymbellales Cymbellacea e Encyonema Cymbellales Cymbellacea e Encyonema Cymbellales Cymbellacea e Encyonema Cymbellales Cymbellacea e Encyonema Cymbellales Cymbellacea e Encyonema Cymbellales Cymbellacea e Encyonopsis Rhopalodiale s

Rhopalodiac e Epithemia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae EunotiaAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Eunotia yanomami Eunotia zygodon Fragilaria Fragilaria brevistriata Fragilaria capucina Fragilaria crotonensis Fragilaria javanica Fragilaria pinnata Fragilaria synegrotesca Fragilaria virescens Fragilariaceae Fragilariforma Fragilariforma bicapitata Fragilariforma virescens Fragilariophyceae Frustulia Frustulia rhomboides Frustulia saxonica Frustulia vulgaris Geissleria decussis Gomphonema Gomphonema affine Gomphonema augur Gomphonema clevei Gomphonema gracile Gomphonema grovei lingulatum Gomphonema guaraniarum Gomphonema minutum Gomphonema parvulum Gomphonema truncatum Gomphonema vibrio Gyrosigma Gyrosigma obscurum Hantzschia Hantzschia amphioxys Hippodonta Hippodonta capitata Hippodonta hungarica Karayevia clevei Kobayasiella Lemnicola hungarica Luticola Luticola mutica Melosira Meridion Microcostatus polonarum Navicula Navicula bouillantensis Order Family Genus Current Name Eunotiales Eunotiaceae Eunotia Eunotiales Eunotiaceae Eunotia Fragilariales Fragilariacea e Fragilaria Fragilariales Fragilariacea e Pseudostaur o Pseudostaurosira brevistriata Fragilariales Fragilariacea e Fragilaria Fragilariales Fragilariacea e Fragilaria Fragilariales Fragilariacea e Fragilaria Fragilariales Fragilariacea e Staurosirella Staurosirella pinnata Fragilariales Fragilariacea e Fragilaria Fragilariales Fragilariacea e Fragilaria Fragilariforma virescens Fragilariales Fragilariacea e Fragilariaceae Fragilariales Fragilariacea e Fragilariforma Fragilariales Fragilariacea e Fragilariforma Fragilariales Fragilariacea e Fragilariforma Fragilariophy c Fragilariophy c Fragilariophyceae Naviculales A mphipleura c Frustulia Naviculales A mphipleura c Frustulia Naviculales A mphipleura c Frustulia Naviculales A mphipleura c Frustulia Naviculales Naviculaceae Geissleria Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Cymbellales Gomphonem a

Gomphonema Naviculales Pleurosigmat a Gyrosigma Naviculales Pleurosigmat a Gyrosigma Bacillariales Bacillariacea e Hantzschia Bacillariales Bacillariacea e Hantzschia Naviculales Naviculaceae Hippodonta Naviculales Naviculaceae Hippodonta Naviculales Naviculaceae Hippodonta A chnanthales A chnanthidia c Karayevia Naviculales Naviculaceae Kobayasiella A chnanthales A chnanthidia c Lemnicola Naviculales Diadesmidac e Luticola Naviculales Diadesmidac e Luticola Melosirales Melosiraceae Melosira Fragilariales Fragilariacea e Meridion Naviculales Naviculaceae Microcostatus Naviculales Naviculaceae Navicula Naviculales Naviculaceae NaviculaAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Navicula clementis Navicula cocconeiformis Navicula constans Navicula cryptocephala Navicula cryptotenella Navicula decussis Navicula elginensis Navicula festiva Navicula gastrum Navicula germainii Navicula heimansioides Navicula ingenua Navicula kotschyi Navicula longicephala Navicula mediocris Navicula minima Navicula occulta Navicula peregrina Navicula placenta Navicula placentula Navicula porifera Navicula radiosa Navicula rhynchocephala Navicula rostellata Navicula schroeteri Navicula seminulum Navicula tripunctata Navicula vaneeii Navicula viridulacalcis Naviculaceae Naviculales Neidium Neidium alpinum Neidium alpinum quadripunctatum Neidium amphigomphus Neidium binodis Neidium densestriatum Neidium dubium Neidium ladogensis densestriatum Nitzschia Nitzschia amphibia Nitzschia clausii Nitzschia dissipata Nitzschia frustulum Nitzschia gracilis Nitzschia intermedia Nitzschia linearis Nitzschia nana Order Family Genus Current Name Cymbellales Cymbellacea e Placoneis Placoneis clementis Naviculales Naviculaceae Navicula Cavinula cocconeiformis Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Geissleria Geissleria decussis Cymbellales Cymbellacea e Placoneis Placoneis elginensis Naviculales Naviculaceae Navicula Cymbellales Cymbellacea e Placoneis Placoneis gastrum Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Chamaepinn u Chamaepinnularia mediocris Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Navicula Naviculales Naviculaceae Naviculaceae Naviculales Naviculales Naviculales Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Naviculales Neidiaceae Neidium Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e NitzschiaAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Nitzschia palea Nitzschia paleacea Nitzschia palustris Nitzschia reversa Nitzschia scalaris Nitzschia seriata Nitzschia serrata Nitzschia subacicularis Nupela Nupela cymbelloidea Nupela neotropica Opephora Pinnularia Pinnularia acrosphaeria Placoneis Placoneis elginensis Planothidium Planothidium apiculatum Planothidium delicatulum Planothidium frequentissimum Planothidium lanceolatum Planothidium peragalli Planothidium rostratum Pleurosira laevis Psammothidium daonense Psammothidium sacculum Pseudostaurosira brevistriata Rhopalodia Rossithidium Rossithidium pusillium Sellaphora Sellaphora laevissima Sellaphora pupula Stauroneis Stauroneis anceps Stauroneis phoenicenteron Stauroneis prominula Stauroneis smithii Staurosira construens Staurosira elliptica Staurosirella Staurosirella leptostauron Staurosirella pinnata Stenopterobia Stenopterobia delicatissima Stephanodiscus Surirella Surirella angusta Order Family Genus Current Name Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Bacillariales Bacillariacea e Nitzschia Naviculales Naviculaceae Nupela Naviculales Naviculaceae Nupela Naviculales Naviculaceae Nupela Fragilariales Fragilariacea e Opephora Naviculales Pinnulariacea Pinnularia Naviculales Pinnulariacea Pinnularia Cymbellales Cymbellacea e Placoneis Cymbellales Cymbellacea e Placoneis A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium A chnanthales A chnanthidia c Planothidium Triceratiales Triceratiacea e Pleurosira A chnanthales A chnanthidia c Psammothidium A chnanthales A chnanthidia c Psammothidium Fragilariales Fragilariacea e Pseudostaurosira Rhopalodiale s

Rhopalodiac e Rhopalodia A chnanthales A chnanthidia c Rossithidium A chnanthales A chnanthidia c Rossithidium Naviculales Sellaphorace a Sellaphora Naviculales Sellaphorace a Sellaphora Naviculales Naviculaceae Navicula Naviculales Stauroneidac e Stauroneis Naviculales Stauroneidac e Stauroneis Naviculales Stauroneidac e Stauroneis Naviculales Stauroneidac e Stauroneis Naviculales Stauroneidac e Stauroneis Fragilariales Fragilariacea e Staurosira Fragilariales Fragilariacea e Staurosira Fragilariales Fragilariacea e Staurosirella Fragilariales Fragilariacea e Staurosirella Fragilariales Fragilariacea e Staurosirella Surirellales Surirellaceae Stenopterobia Surirellales Surirellaceae Stenopterobia Thalassiosiral Stephanodisc Stephanodiscus Surirellales Surirellaceae Surirella Surirellales Surirellaceae SurirellaAs Entered / STORET station -> Sample date ->

Taxon Name / SampleID/PerDiaID -> Synedra Synedra acus Synedra fasciculata Synedra ulna Tabellaria Terpsinoe musica Thalassionema Thalassiosira Tryblionella Tryblionella levidensis Order Family Genus Current Name Fragilariales Fragilariacea e Synedra Fragilariales Fragilariacea e Synedra Fragilariales Fragilariacea e Tabularia Tabularia fasciculata Fragilariales Fragilariacea e Synedra Tabellariales Tabellariacea e Tabellaria Biddulphiales Biddulphiace a Terpsinoe Thalassione m Thalassione m Thalassionema Thalassiosiral Thalassiosira c Thalassiosira Bacillariales Bacillariacea e Tryblionella Bacillariales Bacillariacea e TryblionellaSampleID Peri_IDs STORET Station Nickname Station Description Bioregion WBID Latitude Longitude

22114 8421 22030010 STMARKSREF St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle East 793B 30.2402 -84.14539

25672 10528 22030010 STMARKSREF St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle East 793B 30.2402 -84.14539 47698 20630 BAK210GS BAK210GS St. Mary's River - BAK210GS Northeas t 2211 30.4357 -82.28776

48420 18531 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.5554 -85.435

49652 18430 GAD104US GAD104US Quincy Creek At Sr267 - GAD104US Panhandle West 1303 30.6005 -84.58076 49677 18448 LIB104L V LIB104L V Mule Creek - LIB104L V Panhandle Wes t 684 30.5114 -84.82791 49678 18449 LIB104L V LIB104L V Mule Creek - LIB104L V Panhandle Wes t 684 30.5114 -84.82791 50131 19608 CLA254L R CLA254L R Black Creek - CLA254L R Northeas t 2415C 29.9796 -81.85229

52471 20006 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.2759 -82.73666

52834 21128 273116508208152 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.5213 -82.13756

52912 20031 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.5412 -81.95328
52996 19568 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.9108 -81.88487 52997 19569 CLA243L V CLA243L V Black Creek - CLA243L V Northeas t 2498 29.9108 -81.88487 53033 19609 CLA254L R CLA254L R Black Creek - CLA254L R Northeas t 2415C 29.9796 -81.85229 53059 19639 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.0959 -82.47286 53060 19640 UNI234L V UNI234L V Olustee Creek - UNI234L V Northeas t 3504 30.0959 -82.47286 53732 20850 BAK210GS BAK210GS St. Mary's River - BAK210GS Northeas t 2211 30.4357 -82.28776 53951 20328 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.8139 -81.33541

54263 20007 LEV502GS LEV502GS Waccasassa River - LEV502GS Peninsula 3699 29.2759 -82.73666

54275 20032 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.5412 -81.95328 55073 20329 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.8139 -81.33541 55672 20631 BAK210GS BAK210GS St. Mary's River - BAK210GS Northeas t 2211 30.4357 -82.28776 57324 21790 3513 FLO521150 Withlacoochee River @ Stokes Peninsula 1329C 28.9885 -82.34984

57431 21831 PUT308GS PUT308GS Little Orange Creek - PUT308GS Peninsula 2713 29.5412 -81.95328

57811 22009 GAD104US GAD104US Quincy Creek At Sr267 - GAD104US Panhandle West 1303 30.6005 -84.58076

64546 24394 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3 -82.1 65025 24393 LIB104L V LIB104L V Mule Creek - LIB104L V Panhandle Wes t 684 30.5114 -84.82791

65026 24416 22020093 FLRQUINREF Quincy Creek above SR267 bridge Panhandle West 1303 30.5997 -84.58192 65028 24417 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle Wes t 626 30.5345 -84.77116 65029 24392 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle Wes t 1300 30.4501 -84.86123 65250 24411 CLA246GS CLA246GS Peters Creek - CLA246GS Northeas t 2444 30.0075 -81.74823

65291 24441 3545 S360 S360 Blackwater River @ Hwy 4 Panhandle West 24C 30.8334 -86.73373

65292 24452 32030023 ECONSCTREF ECONFINA CREEK AT SCOTT RD Panhandle West 553 30.5554 -85.435

65293 24458 21010008 HAMIL6 A LAPAHA RIVER 1 SR 150 HAMILTON Panhandle East 3324 30.5999 -83.07342 65296 24444 3546 S365 S365 Yellow River @ Hwy 2 Panhandle Wes t 30 30.9254 -86.55917 65306 24430 BAK208GS BAK208GS St. Mary's River - BAK208GS Northeas t 2097 K 30.5179 -82.23048

65316 24445 3549 S377 S377 Escambia River @ HWY 4 Bridge Panhandle West 10C 30.9641 -87.23599 65379 24479 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle Eas t 3573 A 29.8288 -83.30847

65382 24482 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.8903 -83.25278SampleID Peri_IDs STORET Station Nickname Station Description Bioregion WBID Latitude Longitude

65414 24471 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.1301 -84.49394

65567 24491 23010464 WITHREF Withlacoochee R @ county park off Auton Rd (TP3) Peninsula 1329F 28.3 -82.1 65636 24518 CLA246GS CLA246GS Peters Creek - CLA246GS Northeast 2444 30.0075 -81.74823 65637 24513 UNI234LV UNI234LV Olustee Creek - UNI234LV Northeast 3504 30.0959 -82.47286

65715 24541 WAK158LR WAK158LR Sopchoppy River - WAK158LR Panhandle West 998 30.1301 -84.49394 65731 24544 19010006 STMAR2REF ST MARYS RIVER AT SR #2 Northeast 2097K 30.5179 -82.23031 65736 24548 CLA254LR CLA254LR Black Creek - CLA254LR Northeast 2415C 29.9796 -81.85229 65737 24549 CLA243LV CLA243LV Black Creek - CLA243LV Northeast 2498 29.9108 -81.88487 65739 24551 NUTREF001 TELOG1641 Telogia Creek at CR 1641 Panhandle West 1300 30.4501 -84.86123 65741 24553 GAD106GS GAD106GS Yon Creek - GAD106GS Panhandle Wes t 626 30.5345 -84.77116 65891 24591 GLA630GS GLA630GS Cypress Branch - GLA630GS Peninsula 3235G 26.8139 -81.33541

65900 24599 24010002 MANAREF Manatee R 20 m below SR64 bridge (TP1) Peninsula 1807C 27.4734 -82.21097

65907 24600 273116508208152 EFM02 East Fork Manatee R. S of US62 on Duette Park. Myakka DOSSAC study Peninsula 1811 27.5213 -82.13756 65931 24605 22050083 STEINCANAL Steinhatchee @ Canal Road Panhandle East 3573A 29.8288 -83.30847

65932 24606 22050040 LAFAY1 STEINHATCHEE RIVER I SR357 Panhandle East 3573B 29.8903 -83.25278SampleID Peri_IDs

22114 8421

25672 10528 47698 20630

48420 18531

49652 18430 49677 18448 49678 18449 50131 19608

52471 20006

52834 21128

52912 20031
52996 19568 52997 19569 53033 19609 53059 19639 53060 19640 53732 20850 53951 20328

54263 20007

54275 20032 55073 20329 55672 20631 57324 21790

57431 21831

57811 22009

64546 24394 65025 24393

65026 24416 65028 24417 65029 24392 65250 24411

65291 24441

65292 24452

65293 24458 65296 24444 65306 24430

65316 24445 65379 24479

65382 24482 Sample Date Nreps Gear BacillariophytaP ct ChlorophycotaP ct Cryptophyc ophytaPct Cyanophyc otaPct DiatomNum Taxa DiatomValve Count

8/22/2000 1 Peri-NS 97.39 0.19 0 2.42

9/18/2001 1 Peri-NS 96.39 0.4 0 3.01 11/1/2005 1 Peri-NS 83.73 5.76 0 9.49

2/15/2006 1 Peri-NS 87.7 5.18 0 7.12

11/14/2005 1 Peri-NS 87.33 6 0 6.67 11/21/2005 1 Peri-NS 72.2 9.15 0 18.31 11/21/2005 1 Peri-NS 100 0 0 0 11/14/2005 1 Peri-NS 82.33 1 0 16.67

12/6/2005 1 Peri-NS 89.67 2.33 0 8

6/21/2006 1 Peri-NS 59.32 2.71 0 36.95 44 500

12/13/2005 1 Peri-NS 95.33 1.67 0 3 11/14/2005 1 Peri-NS 93.4 3.96 0 2.64 11/14/2005 1 Peri-NS 100 0 0 0 11/14/2005 1 Peri-NS 100 0 0 0 11/15/2005 1 Peri-NS 97.36 0.33 0 2.31 11/15/2005 1 Peri-NS 100 0 0 0 3/20/2006 1 Peri-NS 88.47 1.36 0 10.17 1/23/2006 1 Peri-NS 63.05 9.49 0.34 22.37

12/6/2005 1 Peri-NS 100 0 0 0

12/13/2005 1 Peri-NS 100 0 0 0 1/23/2006 1 Peri-NS 100 0 0 0 11/1/2005 1 Peri-NS 100 0 0 0 2/20/2007 1 Peri-NS 90.73 0.66 0 8.61 28 500

2/26/2007 1 Peri-NS 86.67 5.67 0 7.33 33 500

11/14/2005 1 Peri-NS 100 0 0 0

5/7/2008 1 Peri-NS 72.09 1 0 26.58 45 500 6/10/2008 1 Peri-NS 99 0 0 1 21 500

6/13/2008 1 Peri-NS 93 2 0 5 44 500 6/13/2008 1 Peri-NS 98 0 0 2 20 500 6/10/2008 1 Peri-NS 85 0.33 0 14.67 36 500 5/28/2008 1 Peri-NS 96.67 1.67 0.67 0.33 26 500

7/9/2008 1 Peri-NS 88.45 2.31 0 2.31 28 500

7/24/2008 1 Peri-NS 89.7 5.65 0 3.65 32 500

7/16/2008 1 Peri-NS 83.44 2.92 0 13.31 46 500 7/9/2008 1 Peri-NS 90.07 1.99 0 7.95 37 500 6/18/2008 1 Peri-NS 90.67 9 0 0.33 14 500

7/10/2008 1 Peri-NS 84.72 5.98 0 9.3 32 500 8/12/2008 1 Peri-NS 60.13 3.65 0 36.21 43 499

8/12/2008 1 Peri-NS 79.67 4.33 0 16 42 500SampleID Peri_IDs

65414 24471

65567 24491 65636 24518 65637 24513

65715 24541 65731 24544 65736 24548 65737 24549 65739 24551 65741 24553 65891 24591

65900 24599

65907 24600 65931 24605

65932 24606 Sample Date Nreps Gear BacillariophytaP ct ChlorophycotaP ct Cryptophyc ophytaPct Cyanophyc otaPct DiatomNum Taxa DiatomValve Count

6/19/2008 1 Peri-NS 81.67 3.67 0 14.67 23 500

10/8/2008 1 Peri-NS 84.59 2.95 0 11.48 50 500 10/28/2008 1 Peri-NS 94 2.67 0 2.67 57 502 10/16/2008 1 Peri-NS 95.77 2.82 0 1.41 55 501

11/13/2008 1 Peri-NS 73.05 4.55 0 22.4 38 500 11/12/2008 1 Peri-NS 93.33 4 0 2.33 44 502 11/6/2008 1 Peri-NS 94.67 1.33 0 4 49 500 11/6/2008 1 Peri-NS 98 1 0 0.33 36 500 11/20/2008 1 Peri-NS 87.69 2.56 0 9.74 62 500 11/20/2008 1 Peri-NS 88 2 0 10 56 500 12/16/2008 1 Peri-NS 91.33 0 0 8.67 33 500

12/17/2008 1 Peri-NS 90 0 0 9.33 28 501

12/17/2008 1 Peri-NS 76 0.33 0 23.67 52 500 1/14/2009 1 Peri-NS 67.45 14.77 0.34 16.44 52 500

1/14/2009 1 Peri-NS 73.33 7 0 19.33 41 500SampleID Peri_IDs

22114 8421

25672 10528 47698 20630

48420 18531

49652 18430 49677 18448 49678 18449 50131 19608

52471 20006

52834 21128

52912 20031
52996 19568 52997 19569 53033 19609 53059 19639 53060 19640 53732 20850 53951 20328

54263 20007

54275 20032 55073 20329 55672 20631 57324 21790

57431 21831

57811 22009

64546 24394 65025 24393

65026 24416 65028 24417 65029 24392 65250 24411

65291 24441

65292 24452

65293 24458 65296 24444 65306 24430

65316 24445 65379 24479

65382 24482 Dominant DiatomPct DominantDiatomPct Word DominantPct

25.14

39.16 83.73

61.81

87.33 72.2 62.4 82.33

89.67

21.8 Fragilariaceae 59.32

95.33
93.4 33.66 29.6 97.36 31.06 88.47 63.05

31

59.2 21.8 71.6 30.4 Melosira 90.73

47 Eunotia 86.67

12.2

20.6 Nitzschia 72.09 29 Eunotia 99

28.8 Navicula 93 53 Navicula 98 15 A chnanthes 85 55.6 Eunotia 96.67

56 Eunotia 88.45

64.4 Eunotia 89.7

15 Diadesmis confervacea 83.44 11 Navicula 90.07 52 Eunotia 90.67

14.8 Planothidium 84.72 23.65 Cocconeis placentul a 60.13

39 Achnanthes rupestoides 79.67SampleID Peri_IDs

65414 24471

65567 24491 65636 24518 65637 24513

65715 24541 65731 24544 65736 24548 65737 24549 65739 24551 65741 24553 65891 24591

65900 24599

65907 24600 65931 24605

65932 24606 Dominant DiatomPct DominantDiatomPct Word DominantPct

40.4 Aulacoseira 81.67

25.4 Nitzschia 84.59 17.33 Eunotia 94 20.56 Nupela 95.77

20.6 Aulacoseira 73.05 20.92 Nitzschia 93.33 23.6 Eunotia 94.67 52.8 Eunotia 98 12.8 Eunotia 87.69 21 Eunotia 88 36.2 Eunotia 91.33

76.65 Melosira 90

19.6 Fragilariaceae 76 35.4 Achnanthes rupestoides 67.45

14 Navicula seminulum 73.33SampleID Peri_IDs

22114 8421

25672 10528 47698 20630

48420 18531

49652 18430 49677 18448 49678 18449 50131 19608

52471 20006

52834 21128

52912 20031
52996 19568 52997 19569 53033 19609 53059 19639 53060 19640 53732 20850 53951 20328

54263 20007

54275 20032 55073 20329 55672 20631 57324 21790

57431 21831

57811 22009

64546 24394 65025 24393

65026 24416 65028 24417 65029 24392 65250 24411

65291 24441

65292 24452

65293 24458 65296 24444 65306 24430

65316 24445 65379 24479

65382 24482 DominantPct Word DominantWet AlgaePct DominantWetAlgaeP ct Word Euglenophyc otaPct Pyrrophycop hytaPct TotalNin d TotalTaxa Sample Comments

Eunotia 0 0 537 30

Cocconeis placentula 0 0 498 34 Bacillariophycea e 0.68 0 295 16

Eunotia 0 0 309 39

Bacillariophyceae 0 0 300 10 Bacillariophycea e 0.34 0 295 17 Eunotia 0 0 500 31 Bacillariophycea e 0 0 300 9

Bacillariophyceae 0 0 300 12

Bacillariophyta 42.5 Jaaginema 1.02 0 295 16

Bacillariophyceae 0 0 300 10 Bacillariophyceae 0 0 303 10 Eunotia 0 0 505 22 Eunotia 0 0 500 32 Bacillariophyceae 0 0 303 4 Pinnularia 0 0 499 30 Bacillariophycea e 0 0 295 9 Bacillariophyceae 4.07 0 295 27

Achnanthes rupestoides 0 0 500 41

Eunotia 0 0 500 29 Eunotia 0 0 500 48 Eunotia 0 0 500 16 Bacillariophyta 25 Oscillatoria tenuis 0 0 302 13

Bacillariophyta 22.5 Jaaginema 0.33 0 300 16

Cocconeis fluviatilis 0 0 500 43

Bacillariophyta 57.14 Schizothrix calcicola 0.33 0 301 9 Bacillariophyta 100 Jaaginema 0 0 300 2

Bacillariophyta 19.05 Synechocystis 0 0 300 10 Bacillariophyta 66.67 Planktolyngbya 0 0 300 4 Bacillariophyta 71.11 Schizothrix calcicola 0 0 300 5 Bacillariophyta 30 Chlorella 0.33 0.33 300 8

Bacillariophyta 57.14 Batrachospermum 0.33 0 303 12

Bacillariophyta 16.13 Synechocystis 1 0 301 17

Bacillariophyta 31.37 Synechocystis 0.32 0 308 17 Bacillariophyta 23.33 Synechocystis 0 0 302 14 Bacillariophyta 50 Mougeotia 0 0 300 7

Bacillariophyta 19.57 Protococcus 0 0 301 12 Bacillariophyta 51.67 A nabaena subcylindric 0 0 301 12

Bacillariophyta 18.03 Jaaginema 0 0 300 16SampleID Peri_IDs

65414 24471

65567 24491 65636 24518 65637 24513

65715 24541 65731 24544 65736 24548 65737 24549 65739 24551 65741 24553 65891 24591

65900 24599

65907 24600 65931 24605

65932 24606 DominantPct Word DominantWet AlgaePct DominantWetAlgaeP ct Word Euglenophyc otaPct Pyrrophycop hytaPct TotalNin d TotalTaxa Sample Comments

Bacillariophyta 72.73 Anabaena 0 0 300 9

Bacillariophyta 25.53 Jaaginema 0.98 0 305 18 Bacillariophyta 22.22 Jaaginema 0.67 0 300 8 Bacillariophyta 66.67 Oedogonium 0 0 71 3

Bacillariophyta 24.1 Schizothrix calcicola 0 0 308 17 Bacillariophyta 15 Jaaginema 0.33 0 300 11 Bacillariophyta 56.25 Schizothrix calcicola 0 0 300 6 Bacillariophyta 33.33 Chlorella 0 0 300 6 Bacillariophyta 29.17 Schizothrix calcicola 0 0 195 10 Bacillariophyta 25 Jaaginema 0 0 300 12 Bacillariophyta 69.23 Schizothrix calcicola 0 0 300 6

Bacillariophyta 50 Jaaginema 0.67 0 300 7

Bacillariophyta 87.5 Schizothrix calcicola 0 0 300 6 Bacillariophyta 22.68 Chlorella 0 0 298 20

Bacillariophyta 23.75 Synechocystis 0.33 0 300 15SampleID Peri_IDs

22114 8421

25672 10528 47698 20630

48420 18531

49652 18430 49677 18448 49678 18449 50131 19608

52471 20006

52834 21128

52912 20031
52996 19568 52997 19569 53033 19609 53059 19639 53060 19640 53732 20850 53951 20328

54263 20007

54275 20032 55073 20329 55672 20631 57324 21790

57431 21831

57811 22009

64546 24394 65025 24393

65026 24416 65028 24417 65029 24392 65250 24411

65291 24441

65292 24452

65293 24458 65296 24444 65306 24430

65316 24445 65379 24479

65382 24482 Ben/Per/Phy /MacPhy Comments

LIMS476051?

lims547453? SID-55672

Ref SCI,Biorecon,water,Periphyton samples taken,SCI shipped to Tall.,Turb. viola

57811?? 49678? 49677 SID-53033

SID-54263

not enough leaves to sample. Neoperla observed

SID-54275
SID-52997 SID-52996?? SID-50131?? SID-53060 SID-53059?? SID-55852 SID-55073

SID-52471??

SID-52912?? SID-53951?? SID-47698??

SID-49652

Habitats have alot of sponge on themAs Calculated / STORET station -> Sample date ->

Taxon Name / SampleID/BenID -> Hydrobiidae Hydrodroma Hydrophilidae Hydroporus Hydropsyche Hydropsyche decalda Hydropsychidae Hydroptila Hydroptilidae Hygrobates Hygrotus Ischnura Ischnura hastata Isonychia Isoperla Isopoda Isotomurus Isotomurus tricolor Kiefferulus Krendowskia Labiobaetis Labrundinia Labrundinia becki Labrundinia johannseni Labrundinia neopilosella Labrundinia pilosella Laevapex Laevapex peninsulae Larsia berneri Larsia decolorata Lauterborniella agrayloides Lebertia Lepidoptera Leptoceridae Leptophlebia Leptophlebiidae Leuctra Libellulidae Limnesia Limnodrilus hoffmeisteri Limnophila Limonia Liodessus

Liodessus affinis Lioporeus Lirceus Littoridinops monroensis Lumbriculidae Lumbriculus variegatus 20030341 20030342 33010065 21030064 10/2/1995 10/2/1995 2/14/1996 2/6/1996 SampleID 7778/BenthID 8855(#counted) SampleID 7779/BenthID 8856(#counted) SampleID 9179/BenthID 10164(#counted) SampleID 9196/BenthID 10181(#counted) 0001 0000 0000 0000 0000 0000 0000 0000 0000 1000 0000 0000 0000 0000 0000 0002 0000 0000 0000 0000 0000 0001 0100 0000 0000 0000 0000 0000 0000 0000 0000 0001 0000 0000 0000 0070 0000 0000 0000 1 62 2 0 0000 0000 0010

0000 0000 0000 0200 0000 0Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A6 -1 

## Appendix A6. FDEP’s Development and Testing of Biomonitorin g Tools for Macroinvertebrates in Florida Streams

Cross

- reference:
Appendix B-2: FDEP’s Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida Streams. U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule. January 14, 2010. EPA- HQ

- OW -2009-0596, FRL-9105-1.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



# DEVELOPMENT AND TESTING OF BIOMONITORING TOOLS FOR MACROINVERTEBRATES IN FLORIDA STREAMS (STREAM CONDITION INDEX AND BIORECON)

Final Report

Prepared for:
Florida Department of Environmental Protection Twin Towers Office Building 2600 Blair Stone Road Tallahassee, FL 32399-2400

Leska S. Fore*, Russel Frydenborg**, Denise Miller**, Tom Frick**, David Whiting**, Julie Espy**, and Loretta Wolfe**
* Statistical Design, Inc. 136 NW 40 th 

## St. Seattle, WA 98107
** Florida Dept. Environmental Protection 2600 Blair Stone Rd. Tallahassee, Fl 32399-2400

March 2007Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### iii 

## This project and the preparation of this report were funded in part by a Section 319 Nonpoint Source Management Program grant from the U.S. Environmental Protection Agency (EPA), through a contract with the Watershed Management Program of the Florida Department of Environmental Protection. The EPA contributed $64,800 to the cost of the project.



# Dedication

On behalf of the Florida Department of Environmental Protection, the Biocriteria

Committee dedicates this document to Dr. James R. Karr.

We would like to thank Dr. Karr for his vision, guidance, and mentoring during the development of its monitoring, assessment, and water quality standards programs. We recognize and appreciate his lifelong dedication to the creation, improvement, and implementation of biological monitoring to protect water resources.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### iv 

# TABLE OF CONTENTS

L IST OF 

## T ABLES



## .................................................................................. VII 

## L IST OF 

## F IGURES



## .................................................................................. IX 

## A CKNOWLEDGMENTS



## ............................................................................. X 

## F OREWARD



## .......................................................................................... XI 

## A BSTRACT



## .......................................................................................... XII 

## C HAPTER 

## 1: I NTRODUCTION



## ................................................................... 1
Backgro und ............................................................................................................. 1

Methods ............................................................................................................. 2 Study area ........................................................................................................... 2

Site selection and dat asets................................................................................ 3
Quantifying human distur bance........................................................................ 4

Stream macroinver tebrate sam pling ................................................................ 8
Metric developmen t and testing........................................................................ 9
Index developmen t and testing....................................................................... 12

Statistical m odel assump tions........................................................................ 13


## C HAPTER 

## 2: R ESULTS



## ......................................................................... 15

Human disturba nce grad ient ................................................................................ 15

Metric sel ection...................................................................................................... 15

Taxonomic richness......................................................................................... 15
Feeding group................................................................................................... 18

Voltinism ........................................................................................................... 18

Habit .................................................................................................................. 18
Community st ructure ....................................................................................... 18
Sensitivity a nd tolera nce

................................................................................. 18Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### v BioRecon me trics............................................................................................. 20

Multimetric index cons truction and evaluati on................................................... 20
Statistical preci sion of indexes ............................................................................ 23


## C HAPTER 

## 3: D ISCUSSION



## ..................................................................... 39

Human disturba nce grad ient ................................................................................ 39
Biological i ndicators.............................................................................................. 39

Sources of variance............................................................................................... 40


## C HAPTER 

## 4: A PPLICATION OF 

## B IOMONITORING 

## T OOLS



## ........................... 42
Regulatory fr amework ........................................................................................... 42 Designate d uses............................................................................................... 42
Water quality criteria ........................................................................................ 43
Anti-degrada tion pol icy ................................................................................... 43
Tiered aquati c life uses ......................................................................................... 44 Determining the impairment thres hold using the Biological Condition Gradient model ................................................................................................. 44
Defining biocriteria using reference site condition....................................... 48

Biocriteria fo r st reams........................................................................................... 51


## C HAPTER 

## 5: C ONCLUSIONS 

## ..................................................................52
R EFERENCES



## ...................................................................................... 53
A PPENDICES



## ....................................................................................... 59
Appendix A. HDG calculati on ............................................................................... 59
Appendix B. Long- lived taxa................................................................................. 63
Appendix C. Se nsitive taxa ................................................................................... 66
Appendix D. Very tolerant taxa............................................................................. 68
Appendix E. Regional calibrati on of metric scores ............................................ 71Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### vi Appendix F. ANOVA output and re gression results for the SCI and BioRecon........................................................................................... 75
Appendix G. Optimal sample si ze for calcu lating SCI ........................................ 77

Refere nces ........................................................................................................ 81
Appendix H. FDEP Biological condi tion gradient workshop summary ............ 82 Backgro und....................................................................................................... 82

Met hods............................................................................................................. 82
Sample Se lection.............................................................................................. 83

Data C ontent ..................................................................................................... 86

Results .............................................................................................................. 86

Refere nces

........................................................................................................ 87
Appendix I. Change Point Analy sis of Stream Condition Index and Biological Condition Gradient Data ................................................ 91 Backgro und....................................................................................................... 91

The Detection Proce dure ................................................................................. 91
Analysis of SCI and BCG Data ........................................................................ 93
Summary ........................................................................................................... 95

Refere nces ........................................................................................................ 95
Appendix J. Refe rence si tes ................................................................................. 97Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### vii 

# LIST OF TABLES

Table 1. Description of various datasets, number of sites sampled, total number of visits to all sites (varied by site), and total number of subsamples taken in the laboratory for all visits .................................................................................. 4 Table 2. Hydrologic condition of stream site, scoring range for hydrologic index, and description of human influences .................................................................... 5 Table 3. Description of land use and the coefficient value used to calculate the LDI.......... 7

Table 4. Correlation table of NH 3 

### (mg/L), hydrologic index, habitat index, LDI for the buffer, LDI for the upstream watershed, natural log of watershed size, HDG, current SCI, and old SCI for 223 stream sites (except NH 3 

### , for which n = 214)..................................................................................................... 8 Table 5. Scoring rules for measures used to calculate the HDG ........................................... 8 Table 6. Candidate metric, correlation with the HDG, and whether the metric was included in the final SCI, BioRecon, or original SCI ........................................... 10

Table 7. Correlation of BioRecon metrics and the old and new BioRecon Indexes with the HDG and SCI ............................................................................................ 21 Table 8. SCI metric name and range of metric values used to assign a score of 0 (indicating degraded condition) to 10 (indicating minimal disturbance) by region................................................................................................................. 21 Table 9. BioRecon metric name and range of metric values used to assign a score of 0 (indicating degraded condition) to 1 (indicating minimal disturbance) by region .......................................................................................... 22 Table 10. Results for seasonal comparison of the SCI and its component metrics........... 36 Table 11. STORET site code, region, station nickname, observed change in BioRecon values through time, and possible reason for change ..................... 37 Table 12. BCG Workshop Participants ................................................................................... 46 Table 13. Statistics and percentile values for the SCI reference sites. ............................... 50 Table 14. Proposed aquatic life use categories, corresponding SCI values, and descriptions of biological conditions typically found for that category........... 51 Table A-1. Laboratory codes for water chemistry measures................................................59

Table E-1. Percentiles for SCI metric values ..........................................................................71 Table E-2. Adjusted metric values used in scoring ...............................................................72 Table E-3. Metric scoring formulae .........................................................................................72Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### viii Table F-1. General linear model output for the SCI versus HDG by region (northeast, panhandle, or peninsula) ................................................................... 75 Table F-2. General linear model output for the SCI versus HDG by year class (1992–93, 1994–95, 1996–97, 1998–99, and 2000–01) ......................................... 75 Table F-3. ANOVA estimates of SCI variability for repeat visits to the same site within a year ........................................................................................................... 76 Table F-4. ANOVA estimates of SCI variability for laboratory subsampling....................... 76 Table F-5. ANOVA estimates of BioRecon variability for repeat visits to the same site within a year .................................................................................................... 76 Table G-1. Percentiles of metric values for the development data set and random sampling in 2004 .................................................................................................... 78 Table H-1. BCG workshop reference sites ............................................................................. 84 Table H-2. BCG workshop samples ........................................................................................ 85 Table H-3. BCG workshop scores summary ..........................................................................88 Talbe H-4. Experts' categorization of BCG workshop scores ..............................................90 Table I-1. SCI and BCG scores for the 30 Samples .............................................................. 93 Table I-2. Confidence Intervals (CI) for the change points of SCI scores............................ 95

Table J-1. Reference site locations. ........................................................................................ 97 Table J-2. Reference sites sample data ................................................................................ 103Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### ix 

# LIST OF FIGURES

Figure 1. EPT and total taxa versus the HDG for two rounds of testing..............................16 Figure 2. Six of 10 SCI metrics plotted against the HDG.......................................................17 Figure 3. Four of 10 SCI metrics plotted against the HDG....................................................19 Figure 4. The SCI plotted against the HDG.............................................................................24 Figure 5. The old and new BioRecon Indexes plotted against the HDG..............................25 Figure 6. The SCI versus HDG by region................................................................................26 Figure 7. The SCI versus catchment area by HDG ................................................................27 Figure 8. The SCI versus HDG by two-year period ................................................................28 Figure 9. Independent verification of the SCI versus HDG ...................................................29 Figure 10. SCI values for repeat visits to the same sites......................................................30 Figure 11. SCI variance components ......................................................................................31 Figure 12. Variance components of the SCI and metrics......................................................32 Figure 13. Summer versus winter SCI, by region ..................................................................34 Figure 14. Variance components of the BioRecon Index and metrics.................................35 Figure 15. Range of BioRecon Index values by site..............................................................38 Figure 16. Linear regression of SCI against BCG scores .....................................................47 Figure 17. Change point analysis for SCI and BCG scores ..................................................48 Figure 18. Number of SCI samples by year and program .....................................................50 Figure A-1 to A-4. Scoring rules for components of HDG.....................................................61 Figure E-1. Metric scores for percent filterer by bioregion...................................................73 Figures G-1 to G-3. SCI scores derived from different sample sizes...................................79Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### x 

# ACKNOWLEDGMENTS

Florida Department of Environmental Protecti on (FDEP) biologists provided comments and guidance throughout the development and testi ng of the stream macroinvertebrate indexes. Ellen McCarron provided administrative support and a regulatory perspective. Eric Livingston provided administrative and contract support. Reviewers from outside FDEP included Michael Barbour (Tetra Tech) , Jeroen Gerritsen (Tetra Tech) , Dr. James Karr (University of Washington), Tony Olsen (U. S. Environmental Protection Agency), Dr. Manuel Pescador (Florida A&M University), and Teresa Thom (U. S. Fish and Wildlife Service–Eglin Air Force Base), who provided many helpful comments. Additional comments from Tom Frick

, Frank Butera

, Sally Levings

, Donald Ray , and the FDEP Biology section improved many aspects of this document and analysis. Data retrievals were performed by Tom Deck

, Shannon Gerardi , and Denise Miller

. Devan Cobb calculated land cover for stream sites. Julie Espy developed datasets and resolved taxonomic questions. Dana Denson

, David Evans (Water and Air Research), Johnny Richardson

, Robert Rutter , and Dr. Robert Wisseman (Aquatic Biology Association) provided definitions for long-lived taxa. Michael Heyn provided additional clinger information. Lab subsampling was done by Ashleigh Jordan

, Nathalie Moise

, Cathy Oaks

, Brad Richardson

, Brian Shinner , and Felicia Whiddon . Cover photo courtesy of Jeff Adams (Xerces Society). The following biologists throughout Florida coll ected and identified stream macroinvertebrate samples:

Central District (Orlando)

• Dan a Denson

• Phil Kane

• Steve Kent

• Eric Pluchino

Northeast Distri ct (Jacksonville)

• Lee Ban ks

• Patrick O’Connor

Northwest District (Pensacola)

• Fran k Butera

• Glenn Butts

• Lauren ce Do nelan

• Donald Ray

South District (Punta Gorda)

• Rob ert Rutte r

• Albert (Ford) Walton Southeast District (Port St. Lucie)

• Mark Thom pson
Southwest District (Tampa)

• Charles Ko v ach

• Peggy Morgan

• Mary Szafraniec

Tallahass ee Office

• Tom Deck

• Julie Espy

• Ken Espy

• Tom Frick

• Russel Frydenborg

• Shannon Gerardi

• Michael Heyn

• Lisa Hom ann

• Joy Jackson

• Liz Miller

• Johnny Richardson

• Cheryl Swanson

• Amy Wheeler

• Emily SpringerDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### xi 

# FOREWARD

The original version of this document wa s completed in 2004. Subsequently, important observations made during the routin e application of thes e bioassessment tools, as well as new EPA guidance for formulating biological criteria, prompted DEP to revise the document in two key areas. First, the thresholds for interpreting the final Stream Condition Index values have been revised. This was partially due to responses from DEP biol ogists, who noted that many reference streams, formally evaluated as “excellent” using the pre-2004 SCI criteria, were now considered “fair”, or sometimes “poor” using the SCI 2004 thresholds . Additionally, in 2006, EPA promoted using the Biological Condition Gradient approach as a nationally standardized and scientifically robust method for developing defensible biocriteria. Using this app roach, and a second line of evidence concerning the variability of the reference condition, new SCI thresholds have been devised. An additional revision to the document address ed measurement error of the SCI related to laboratory subsampling methods which were subsequent ly modified to improve the precision of the SCI without substantially increasing the cost. This invo lved increasing the number of individuals examined and averaging the results from two independent aliquots from a field sample. Science is an iterative process: incrementa l improvements are made as new evidence and experience accumulate. Such was the motivation behind the revisions made in the original document and as a result of additional statistical analyses cond ucted during 2004–2006. This document represents the work and expertise of many people, all dedicated to applying the best science possible for the protection of Florida’s stream resources.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### xii 

# ABSTRACT

The Florida Department of Environmental Prot ection assesses the chemical, physical, and biological condition of hundreds of stream sites each year. This study used measures of hydrologic condition, riparian and channel habitat condition, water chemistry, and intensity of human land use to define a gradient of human disturbance for stream sites. We evaluat ed the sensitivity and tolerance of over 1,000 stream macroinvertebrat e taxa using the human disturbance gradient (HDG). We tested for correlation between the HDG and 36 biological measures of the stream macroinvertebrate assemblage (metrics), and selected the 10 most highly correlated metrics within 6 categories of biological organization: taxonomic richness (total number of taxa, number of Trichoptera taxa, and number of Ephemeroptera taxa); feeding group (per centage filterer individuals); volt inism (long-lived taxa richness); habit (clinger taxa richness); community structure (percentage dominance of the most abundant taxon and percentage Tanytarsini midges); and sensitivity and tolerance (sensitive taxa richness and percentage very tolerant individuals). Metrics were combined into an overall Stream Condition Index (SCI) by transforming metric values into unitless scores and summing the scores . The SCI was highly correlated with the HDG for an independent dataset (Spearman’s r = –0.81, p < 0.001). The SCI was independent of watershed size and geographic region (Florida panhandle, peninsula, and nor theast). Across 10 years of sampling, the index showed a similar response to the HDG. The SCI was somewhat higher for winter versus summer samples (3.5%). To summarize the statistical precision of the SCI, we used confidence intervals to calculate that the SCI could distinguish 3.7 categories of biol ogical condition. These results supported subsequent selection of three assessment categories for interp reting SCI values in a r egulatory context. A large portion of the variability of the SCI was due to subsampling in the laboratory (49%). To reduce this variability, laboratory sorting procedures have subs equently been modified to target two aliquots of 150 individuals, with the resulting SCI values averaged. We also tested biological metrics for a second st ream macroinvertebrate sampling protocol (the BioReconnaissance Index, or BioRecon), which was bas ed on the sorting of invertebrates in the field and taxonomic identification in the laboratory. Of the 10 SCI metrics, 6 taxa richness metrics were tested using BioRecon data; all were highly correlated with both the SCI and HDG. From these metrics, the BioRecon Index was calculated as the sum of scores fo r the 6 metrics. The stat istical precision of the BioRecon index was sufficient to detect 2.5 categories of biological condition which supported the subsequent decision to select three assessment categories for regulatory purposes. We primarily followed the EPA 2006 Biological Condi tion Gradient approach for translating index values into biocriteria for Florida streams. For this approach, a panel of 22 regional scientists examined data representing multiple tiers of aquatic life use for the ultimate purpose of defining biological criteria for state water quality standards. In addition, the experts recommended a threshold of biological condition at which impairment occurs as a result of human distur bance. A secondary, but parallel analysis based on departure from reference condition yielded similar inde x values for the impairment threshold. Based on these complementary results, three categories of aqu atic life use are proposed for Florida streams and described as Category 1 (“except ional” ), Category 2 (“healthy”), and Category 3 (“impaired”).Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 1 

# CHAPTER 1: INTRODUCTION

Florida, along with a handful of other st ates, leads the nation in the development and implementation of water protection policies based on biological criteria (McCarron and Frydenborg, 1997; U. S. Environmental Protection Agency [EPA], 2002) . Under the federal Clea n Water Act, states are required to define designated uses for specific waterb odies and develop criteria to protect them (Karr, 1991; Ransel, 1995). Over time, the emphasis has sh ifted from what was primarily the chemical monitoring of pollutants to include the direct meas urement of the condition of the biological assemblage (Yoder and Rankin, 1998). Florida classifies its surface waters according to their designated uses. The five categories are ranked in order of the level of protec tion that they require, as follows:

Class I–Drinking water; Class II–Shellfish propagation or harves ting (primarily for coastal waters); Class III–Recreation and propa gation and maintenance of a healthy, well-balanced population of fish and wildlife; Class IV–Agricultural water; and Class V–Navigation and industrial use.

This document describes the development and testing of biological monitoring tools for macroinvertebrate stream samples for the assessment of freshwater streams that are applicable to Class I and III waters. FDEP has convened a Designated Uses Policy Advisory Committee (DUPAC) to explore an alternative system of designated use classificati on for Florida (see Chapter 4 below). We anticipate that FDEP will propose Aquatic Life Use (AL) catego ries, to which these biological monitoring tools for Class I and III streams would also apply. The EPA has developed numerous guidance docume nts to support biological assessment (EPA, 1998; Barbour et al., 1999; Bowman et al., 2000; Jackson et al., 2000; EPA, 2001; EPA, 2002; EPA, 2003a, b, c; Fore, 2003). These documents typically emphasize multimetric indexes to measure the biological condition of surface waters (Karr, 198 1; Karr et al., 1986; Barbour et al., 1999). The EPA’s recent document, Draft Report on the Environment 2003, specifically recognizes multimetric indexes as an “approach that addresses the need to measure critical multiple variables” (EPA, 2003a). Multimetric indexes are composed of biological meas ures, called metrics, that show a consistent response to human disturbance (K arr and Chu, 1999). Metrics are c onverted to unitless scores and summed to obtain a summary index. Multimetric indexes have been developed for fish, birds, invertebrates, and algae in a variety of ecological contexts in North America, Europe, and Asia (Thorne and Williams, 1997; Hughes et al., 1998; Hughes and Oberdorff, 1998; Karr and Chu, 1999; Bryce et al., 2002; Fore and Grafe, 2002; Klemm et al., 2003). Most states use multimetric indexes for biological assessment, although other techniques have been developed (Haw kins et al., 2000; EPA, 2003b, c).


# Background
Barbour et al. (1996) developed multimetric indexes for Florida that have been used since the early 1990s. The authors tested metrics by comparing values at sites with minimal human influence (reference sites) and sites with known disturbance (test sites). They identified three unique geographic regions based on the presence of shared taxa and defined metric expectations for each region. In contrast, this study selected metrics on the bas is of their correlation with a gradient of human disturbance (Bryce et al., 1999). The sensitivity and tolerance of over 1,000 taxa were also evaluated individually, using the human disturbance gradient (HDG). We used confidence intervals to estimate theDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 2 precision associated with the indexes. Working with a panel of regional experts, aquatic life use categories were derived to support management decision related to listing stream sites as impaired and prioritizing sites for additional evaluation as part of the TMDL (Total Maximum Daily Load) process (Karr and Yoder, 2004). From the original analysis by Barbour et al. ( 1996), two multimetric indexes were developed for Florida streams based on different sampling and iden tification protocols. The Stream Condition Index (SCI) was derived from laboratory processing and the identification of samples, while the Biological Reconnaissance (BioRecon) Index was based on field sorting and laboratory identification. This study develops new bioassessment tools for both sampling pr otocols. For simplicity’s sake, the names of the indexes have been retained for this new analysis, and t he previous indexes are referred to as the old SCI and the old BioRecon Index within this document.



# Methods

Study area
Portions of Florida have been repeatedly inundated by seawater in recent geologic history; as a consequence, elevation and organism recruitment from adjacent, noninundated areas play an important role in the present-day geographic distributi on of macroinvertebrates. Florida can be divided geographically into the northern panhandle, the sout hern peninsula, and a transitional region known as the northeast. The middle and lower Suwannee Ba sin provides a natural demarcation between the panhandle and peninsula, with the northeast region str addling the upper Suwannee northeast of the Cody Escarpment (White, 1970). In the Florida panhandle, rivers typically flow from north to south, and the elevation of headwater streams typically exceeds 200 to 250 feet. The recruitment of stream organisms (over geologic time) from upstream, higher elevation areas (Georgia and Alabama) makes the panhandle richer in freshwater stream taxa than other parts of the state (Florida Natural Area s Inventory [FNAI], 1990). Vegetation communities in the panhandle generally cons ist of mixed pine/oak/hickory forests ( Pinus spp., Quercus spp., Carya spp.); longleaf pine forests ( Pinus palustris ); hardwood forests with a beech/magnolia climax community ( Fagus grandiflora / Magnolia grandiflora ); and swamp hardwood forests of cypress ( Taxodium spp.) or tupelo ( Nyssa spp.), interspersed with a mosaic of pine plantations, cropland (e.g., corn, soy beans, and peanuts), and pasture (Soil and Water C onservation Society (SWCS), 1989; Fernald and Purdum, 1992). The panhandle is less densely populated by humans than the other areas. The peninsula has a sandy highland ridge extending down its center almost to Lake Okeechobee. The elevation of the central ridge is approximately 150 to 200 feet. Rivers west of the ridge flow into the Gulf of Mexico. Of the two large drainages east of the ridge, the Kissimmee River flows south into Lake Okeechobee, and the St. Johns River flows north to th e Atlantic. Streams in the peninsula tend to have lower water velocity, flowing through wetlands and pine flatwoods (White, 1958). The peninsula has fewer freshwater invertebrate taxa due to the lack of ups tream recruitment sources that did not experience marine inundation. Plecoptera, for example, are common in the panhandle but extremely rare in the peninsula, probably due to a combination of poor recruitment, warmer temperatures, and lower water velocities. Peninsular vegetation communities on t he ridge consist of longleaf pine/turkey oak forests ( Pinus palustris/Quercus laevis ); on flat areas are slash pine ( Pinus eliottii ) or loblolly pine ( Pinus teada ) with a palmetto/gallberry understory ( Serenoa repens/Ilex glabra ); and in depressional areas are marsh/wet prairies (maidencane, pickerel weed) and hardwood wetlands of sweetbay ( Magnolia virginiana ), cypress ( Taxodium spp.), and ash ( Fraxinus spp.; SWCS, 1989). The dominant land use is pasture, cropland (e.g., watermelons, nursery products, and tomatoes), and urban areas (Fernald and Purdum, 1992). Dense population centers are located at Tampa and Orlando.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 3 The northeast region includes portions of t he Okeefenokee Swamp, parts of the upper Suwannee drainage, the Black Creek drainage, and the Sea Island flatwoods. From the perspective of stream invertebrates, this region tends to be intermediate between the panhandle and peninsula regions. With the exception of the Black Creek Basin, with headw aters approximately 200 feet above sea level, other streams in this area tend to be low-relief, wetland systems (Snell and Anderson, 1970). Plant communities consist of longleaf pine/turkey oak ( Pinus palustris/Quercus laevi) on the sandy uplands; hardwood wetlands of cypress ( Taxodium spp.), tupelo ( Nyssa spp.), and loblolly bay ( Gordonia lasianthus ); pine flatwoods; and marsh (FNAI, 1990). Jack sonville is the region’s only major population center. Barbour et al. (1996) used macroinvertebrate stream samples collected from sites with minimal human influence to evaluate the influence of geographic locations on species assemblages. The authors found that the panhandle, peninsula, and northeast re gions, which are primarily defined according to drainage patterns, were better predictors of species assemblages than were subecoregions described by Griffith et al

. (1994). Regional differences translate into ex pectations of higher taxonomic diversity in the panhandle relative to the peninsula, with the northeast region representing a transitional area.


## Site selection and datasets
The Florida Department of Environmental Prot ection (FDEP) has collected thousands of macroinvertebrate samples from stream sites thr oughout the state from 1991–2001. Many sites have been visited repeatedly. Sites and visits were selected fr om this large database using different criteria for different testing situations (Table 1). For example, when testing for metric correlation with disturbance, a range of site conditions was needed; to evaluate variab ility, sites with many repeat visits were needed; to evaluate laboratory subsampling, large samples we re needed. Some datasets contained data from the same sites, and other sites were selected to prov ide an independent test. For some testing situations, data from site visits were averaged by sites—for example, when one variable in the analysis had only one value for each site, such as the HDG. The relationship between human disturbance and biological condition was evaluated in several ways. To develop the HDG, FDEP biologists selected sites from their regions that represented the broadest possible range of human influence. First, to ensure that the relationships observed between human land use and biological metrics were consistent and not specific to a particular set of sites, 2 independent sets of sites were tested. These data were then combined to obtain 223 sites to test for metric correlation with the HDG. An additional, i ndependent set of 23 sites was used to test the correlation between the SCI and HDG. Additional datasets were compiled to estimate the variability of the biological metrics and indexes associated with repeat visits to a site, estimate the variability associated with laboratory subsampling, and evaluate the influence of field sorting versus laboratory sorting. To evaluate SCI variability, data from 62 sites with 2 to 8 visits were selected. About half the si tes (32) had repeat visits on the same day. Most of the other repeat visits were during different years, an d a few were within the same year. Thus, these data could not be used to estimate the influence of seasons on variability. Instead, additional data were compiled to test for seasonal (winter versus summer) differences in the SCI. To test the influence of subsampling during laboratory processing for the SC I protocol, 3 subsamples were selected from 59 samples collected from 54 sites (5 sites also had 2 duplicate samples each). To test metrics and evaluate their variability for the BioRecon protocol, 2 additional datasets were used. The first dataset included 116 sites with macroi nvertebrate samples collected according to both the BioRecon and SCI sampling protocols. We tested BioRecon metrics for correlation with the SCI and HDG. Most sites had at least 2 visits, and several had 3 to 6 visits. Of the 116 sites, 53 had values calculated for the HDG because they were used initially to develop the HDG. To evaluate the variability of the BioRecon metrics and index, 128 sites were selected, with 2 to 7 visits per site.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 4 The dataset used to test the SCI and its co mponent metrics against the HDG was large; therefore, simple scatter plots of data against the HDG would be difficult to interpret with the many data points and overstrikes. Consequently, we used box plots to display the range of values associated with different values of the HDG. In all figures, the box defined the 25 th and 75 th percentiles, the whiskers were the nonoutlier range, and the outliers were calculated as values greater (or less) than 1.5 times the length of the distance from the 25 th to the 75 th percentile value. During 1991–2001, laboratory protocols mandated a target number of 100 individuals; howev er, this target was often exceeded due to the laboratory protocols of the time. Several graphs incl ude data from samples with 75 to 175 individuals in order to more closely match the target sample size. A more restrictive range (e.g., 75–125 individuals) would have resulted in too high a loss of sites for te sting. Additional analysis (not shown) indicated that the ranges of metric values were similar for an upp er limit of 125 and 175 individuals. In recent years (2004–2006), FDEP has modified laboratory protocol s to more often match the target number of individuals (see Appendix G) . For the BioRecon testing, the number of individuals identified was not recorded; therefore, all site visits were used with in a particular analysis, unless specifically stated otherwise.

Table 1. Description of various datasets, number of sites sampled, total number of visits to all sites (varied by site), and total number of subsamples taken in the laboratory for all visits Some sites were included in more than 1 dataset, exc ept for the 23 independent sites used to verify the correlation between the SCI and HDG.

Purpose Sites Visits Lab Subsamples Test HDG consistent for 2 rounds of data
Round 1 154 469 0
Round 2 69 160 0
Test metrics vs. HDG 223 629 0 Score metrics (same data as above, but only samples with 75 to 175 individuals included) 176 420 0

Test sensitive and tolerant taxa versus HDG 226 632 0 Verify SCI with independent data 23 23 0 Determine SCI variability 62 220 0
Determine seasonal variation of SCI 78 578 0 Evaluate laboratory subsampling variability for SCI 54 59 177 (3 each)
Calibrate BioRecon metrics with SCI 116 165 0
Test BioRecon metrics versus HDG 53 0 Evaluate BioRecon variability 128 293 0



## Quantifying human disturbance
Karr and colleagues describe five factors to summarize the ways in which humans alter and degrade rivers and streams (Karr et al., 1986; Karr et al ., 2000): flow regime, physical habitat structure, water quality, energy source, and biological interactions . First, the natural flow regime of a stream may be altered by dams, water removal, or water return. In extreme cases, changes in water volume or flow timing can result in flooding or dry channels. Second, stream macroinvertebrates depend on a variety of physical habitat types for food, shelter, and reproduction. A greater diversity of substrate size, types of vegetation, and bank architecture tr anslates into a greater variety of organisms. Third, water quality mayDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 5 be evaluated in terms of turbidity, conductivity, nutrient concentrations, or the presence of other chemicals or metals. Fourth, energy source describes the seasonal availability, type of organic material, and size and shape of material available as food. Fi fth, the introduction of exotic species or disease and the harvest of fish and shellfish for sport, commercial, and subsistence uses can alter biological interactions such as competition or predation within the natural assemblage. Data were available to measure four of the five factors. FDEP biologists developed a hydrologic scoring system based on their knowledge of water removal, patterns of drought, and hydrographs for the sites (Table 2). To measure habitat condition, FDEP biologists routinely calculate a habitat condition index whenever stream macroinvertebrate sample s are collected (FDEP, 2004). The index evaluates substrate condition and availability, water veloci ty, habitat smothering (e.g., by sand and silt), channelization, bank stability, and the width and conditi on of riparian vegetation. During the period of data collection, the habitat index was modified to include additional measures that raised the total number of possible points. To make habitat condition comparable across years, the total habitat score was converted to a percentage by dividing t he score by the total possible points.

Table 2. Hydrologic condition of stream site, scoring range for hydrologic index, and description of human influences The high or low values within each condition class were assigned at the discretion of the biologist based on the extent of disturbance. These changes were associated with human disturbances, not natural events such as hurricanes or extreme droughts.

Condition Score Description

Excellent 1–2 Flow regime as it naturally occurs (slow and fairly continual release of water after rains), few impervious surfaces; high connectivity with ground water and surface features delivering water (e.g., sandhills, wetlands; no ditches or berms)

Good 3–4 Flow regime minimally changed; some water withdrawals; some wetland drainage, some impervious surfaces, some ditching

Moderate 5–6 Flow regime moderately altered; hydrograph moderately flashy (scouring after rain events with subsequent reductions in flow), ground water pumping evident; much wetland drainage, topographic alterations reduce natural water input; more impervious surfaces, dam s/control structures change normal water delivery schedule

Poor 7–8 Flow regime highly altered; hydrograph very flashy (scouring after rain events with subsequent reductions in flow, leading to stagnant or dry conditions related to large amounts of impervious surfaces and/or ditching); water withdrawals and impoundments or control structures severely alter flows; large areas of impervious surfaces

Very poor 9–10 Flow regime entirely human controlled; hydrograph very flashy (scouring after rain events with subsequent reductions in flow, leading to stagnant or dry conditions related to impervious surfaces and ditching); intensity of water withdrawals and impoundments fundam entally alter the nature of the ecosystem

FDEP biologists also routinely collect measurements of turbidity, temperature, dissolved oxygen, conductivity, and nutrient concentration s. Total phosphate (TP), ammonia (NH 3 ), total Kjeldahl nitrogen (TKN), and nitrites/nitrates (NO x ) were measured to summarize nutrients. We selected NH 3 to summarize water quality because it was the mo st consistently associated with the other water quality measures and had the most complete record of data. TP had similar high correlations and complete data, but may beDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 6 more closely associated with fertiliz er and farming practices, while NH 3 may be a more general indicator of both urbanization and agriculture. A measure of the type and quantity of energy avail able to organisms living within the stream was not available. However, Brown and colleagues have de veloped an index to estimate the intensity of human land use based on nonrenewable energy flow (B rown et al., 1998; Brown and Vivas, 2005). The Landscape Development Intensity (LDI) Index was calculated as the percentage area within the catchment of particular types of land use multiplied by the coefficient of energy use associated with that land use, summed over all land use types in the catchment (Table 3). 

# ( ) ∑ = i i 

## LULDI LDI %*

.

Where:

LDIi = the nonrenewable energy land use for land use i, and %LUi = the percentage of land area in the catchment with land use i.

Brown and colleagues derived the coefficients for each land use type from actual billing records and published literature, translated reported energy use into standardized units, and then averaged the values by land use type and standardized to a per -unit area. The calculations used only nonrenewable energies, which included electricity, fuels, fertilizers, pesticides, and water (both public water supply and irrigation). Land use was derived from aerial photos manipulated as layers in a geographic information system (GIS) computer program. The LDI was calculated in 2 ways. First, a buffer area of 100 meters on each side of the stream and 10 kilometers upstream of the sampling point was used (LDI_BF). Because the definition of the buffer region was somewhat arbitrary, the LDI was also calculated for the entire upstream catchment (LDI_WS) in order to determine which spatial scale was a better predictor of site condition. We selected LDI_BF to include in the HDG because it was slightly more highly correlated with NH 3 , the hydrologic index, and the habitat index (Table 4). To define the HDG, we convert ed the 4 measures of human disturbance to unitless scores and summed the scores to create HDG values for each stream site (Appendix A) . Three of the measures had values of 0, 1, or 2, indicating low, moderate, or high levels of human influence. One measure, hydrologic condition, had an additional value of 4 available to indicate extreme levels of disturbance (Table 5). Thus, values for the HDG ranged from 0 (indicating mi nimal human disturbance) to 9 (indicating extreme disturbance). The scores for the HDG were derived from a graphical analysis of EPT taxa richness plotted against each measure of disturbance. We identified values for each disturbance measure for which the number of EPT taxa was consistently lower. For NH 3 , 9 sites had no information. Most of these sites had values for other measures indicating minimal human disturbance. We assumed for these sites that NH 3 was less than 0.1 milligram per liter (mg/L).Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 7 Table 3. Description of land use and the coefficient value used to calculate the LDI

Higher values indicate greater intensity of human land use (Brown and Vivas, 2005).

Land Use LDI Value Natural Open Water 1.00 Natural System (no human activity) 1.00
Pine Plantation 1.58
Woodland Pasture 2.02
Pasture 2.77 Recreational/Open Space (low-intensity) 2.77

Low-Intensity Pasture (with livestock) 3.41 Citrus 3.68 High-Intensity Pasture (with livestock) 3.74 Row crops 4.54 Single-Family Residential (low-density) 6.79 Recreational/Open Space (high-intensity) 6.92 High-Intensity Agriculture 7.00 Single-Family Residential (medium-density) 7.47

Single-Family Residential (high-density) 7.55 Low-Intensity Highway 7.81 Low-Intensity Commercial 8.00
Institutional 8.07 High-Intensity Highway 8.28
Industrial 8.32 Low-Intensity Multifamily Residential 8.66 High-Intensity Commercial 9.18 High-Intensity Multi-fa mily Residential 9.19 Low-Intensity Central Business District 9.42 High-Intensity Central Business District 10.00Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 8 Table 4. Correlation table of NH 3 (mg/L), hydrologic index, habitat index, LDI for the buffer, LDI for the upstream watershed, natural log of watershed size, HDG, current SCI, and old SCI for 223 stream sites (except NH 3 , for which n = 214) Repeat visits to individual sites were averaged for each site. All values shown were significant (Spearman’s r, p < 0.01).

NH 3 Hydro Habitat LDI_BF Ln (Size) HDG SCI
NH 3 - 0.43 –0.45 0.39 –0.20 0.50 –0.56 Hydro 0.43 - –0.66 0.61 –0.36 0.84 –0.71
Habitat –0.45 –0.66 - –0.64 0.41 –0.78 0.66 LDI_BF 0.39 0.61 –0.64 - –0.37 0.79 –0.60 LDI_WS 0.34 0.56 –0. 55 0.74 –0.28 0.70 –0.59 Ln (Size) –0.20 –0.36 0.41 –0.37 - –0.41 0.36

HDG 0.50 0.84 –0.78 0.79 –0.41 - –0.76
SCI –0.56 –0.71 0.66 –0.60 0.36 –0.76 - Old SCI –0.43 –0.59 0. 56 –0.54 0.29 –0.69 0.86

Table 5. Scoring rules for measures used to calculate the HDG The HDG is the sum of the scores for each site.

Measure 0 1 2 3
NH 3 (mg/L) <0.1 0.1–2 >2 Habitat index (% of total possible points) >65 50–65 <50 Hydrologic index <6 6–7 8–9 10
LDI <2 2-3.5 >3.5



## Stream macroinvertebrate sampling
FDEP uses two protocols to collect invertebrate samples from streams. The protocols differ in the number of dip net sweeps collected, the location of sample sorting (field versus laboratory), and the final index used for assessment. The BioRecon sampling protocol was designed to be a quick field assessment that could yield a same-d ay measure of stream site condition. The SCI sampling protocol was meant to be more precise. Both protocols us e a D-frame dip net with a 0.25-meter opening and a 600-micrometer mesh net. Both protocols distribute the sweeps among the best available substrates on an approximately equal basis. Florida streams typicall y have a sandy substrate that supports very few invertebrates. Therefore, the habitat sampled for ma croinvertebrates includes logs, roots, and undercut banks; partially decayed leaf packs; a quatic vegetation; and rocks or large cobble. During SCI sampling, if 4 productive substrates are present (snags, roots, vegetation, and leaf packs), each substrate would be sampled 4 times, with sand being sampled 4 times, for a total of 20 sweeps. In the laboratory, the SCI sample is divided by spreading it onto gridded trays. Squares are randomly selected, and all the material within a square is sorted. Macroinvertebrates are identified to the lowest practical taxonomic level. For the 2004 to 2006 time period, squares were selected until the target sample size of 100 individuals was reached. To avoid bias, the final square selected is always finished,Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 9 even if it contains more than 100 individuals. T he current method targets two separate aliquots of 150 individuals. The number of taxa and the relative abundance of each taxon are derived from these data. BioRecon data are collected using four dip net sweeps from a stream site. Samples are sorted for live macroinvertebrates in the field. The sample is spread in a pan, and the biologist searches through all the material for every unique taxon present. Each ta xon is preliminarily identified in the field, and representative specimens of each taxon are pres erved and returned to the laboratory for final identification under a dissecting microscope. From this field-sort protocol, the num ber of taxa present at a site can be measured, but their relative abundance (% composition) cannot. 

## Metric development and testing

Several authors have described relevant c andidate metrics for str eam macroinvertebrate assemblages (Barbour et al., 1996 and 1999; Kerans and Karr, 1994; and Klemm et al., 2002). For this study, we evaluated 36 candidate metrics related to taxonomic richness, feeding group, voltinism, habit, community structure, and tolerance or sens itivity for their association with the HDG (Table 6). Within each category, we selected metrics with the highe st correlation with the HDG (Spearman’s r). We used onesided tests to calculate p- values because if a metric indicated better biological condition in response to increased human disturbance, t hat relationship would not be biologically significant even if it were statistically significant. All metrics were also eval uated graphically to ensure that they were reliable indicators of disturbance. We tolerated variability in metric values for minimally disturbed sites (low HDG) because not all sources of disturbance were known. For example, water chemistry data were unavailable for metals, pesticides, or other toxics. In contrast, we considered an inconsistent response at more highly disturbed sites to be unacceptable.



### Taxonomic richness Richness was calculated as the number of unique ta xa found within a particular group, such as the Ephemeroptera, Plecoptera, or Tric hoptera, or their combination (EPT).


### Feeding group Trophic designations followed Merritt and Cummi ns (1996); FDEP biologists and others modified some designations to reflect natural histories in Florida (Merritt et al., 1996). All feeding group metrics were calculated as the number of individuals of a particular group, divided by the total number of individuals in the sample times 100 (percentage).



### Voltinism Most stream insects spend the majority of their lives as larvae underwater and emerge as adults to mate. The length of time between generations, wh ich is called voltinism, may be less than a year, approximately one year, or greater t han one year. We used published sources to identify taxa that require more than one year to complete their life cycles ( Appendix B ; Brigham et al., 1982; Thompson, 1984; Thorp and Covich, 1991; Pescador et al., 1995; Co rbet, 1999; Pescador et al., 2000; Smith, 2001; Rasmussen and Pescador, 2002). These taxa require consistent flows and other favorable conditions throughout the year to complete their life cycles. Data on generation length were not available for all Florida taxa; therefore, the current list may not include some long-lived taxa.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 10 Table 6. Candidate metric, correlation with the HDG, and whether the metric was included in the final SCI, BioRecon, or original SCI Correlation was tested for 223 sites, with repeat visi ts to each site averaged before statistical testing. Hyperabundance was tested without averaging, n = 577. All correlations shown were significant at p < 0.01 (Spearman’s r, one-sided test).

Metric HDG SCI BioRecon Old SCI
Taxonomic richness EPT –0.73 yes Trichoptera –0.66 yes yes Ephemeroptera –0.66 yes yes Total taxa –0.52 yes yes yes

Diptera –0.44 Chironomidae –0.40 yes

Plecoptera –0.37 Oligochaeta 0.32

Noninsect - Feeding group % Filterer –0.46 yes yes % Scavenger –0.38 % Browser/grazer –0.30 % Collector/gatherer 0.29 % Predator –0.21 % Plant piercer –0.18

% Parasite - % Scraper - % Shredder - Voltinism Long-lived taxa –0.41 yes yes

% Long-lived –0.35 Habit Clinger taxa –0.71 yes yes % Clinger –0.61 Community structure % Dominance 0.47 yes yes Hyperabundance - % Ephemeroptera –0.58

% Trichoptera –0.55 % Tanytarsini (Chironomidae) –0.45 yes % Plecoptera –0.37 % Oligochaeta 0.32 % Non-insect 0.32 % Chironomidae - % Diptera - yes Sensitivity/tolerance Sensitive taxa –0.75 yes yes Florida index (sensitive) –0.71 yes % Very tolerant 0.70 yes Very tolerant taxa 0.61Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 11 The list included all taxa in the family Cordulegastridae and selected taxa in the families Aeshnidae, Gomphidae, and Libellulidae (Odonata); t he family Leuctridae and selected genera in the families Perlidae and Pteronarcidae (Plecoptera); the family Corydalidae (Megaloptera); and the genera Ceraclea, Pycnopsyche, Molanna, and Rhyacophila (Trichoptera). Several Coleoptera taxa (beetles) also require more than a year to complete their life cy cles, but were not included because the FDEP database does not currently distinguish between larvae (which cannot travel to find water) and adults (which can). Noninsects included in the long-lived list were all taxa in the family Decapoda, the genera Fossaria and Pomacea (Gastropoda), a nd the families Unionidae and Corbiculidae (Pelecypoda).



### Habit Other studies have noted a strong correlation between the taxonomic richness of clingers and stream condition (Fore et al., 2001; Karr and Morish ita Rossano, 2001; Mebane, 2002). Clingers have morphological and behavioral adaptations that allow them to cling to substrate (Merritt and Cummins, 1996). Channel dredging and the removal of ripari an vegetation eliminate t he habitat preferred by clingers.


### Community structure Percentage dominance was calculated as the numbe r of individuals in the most abundant taxon, divided by the total number of individuals in the samp le. ”Hyperabundance” represented a measure of the density of stream macroinvertebrates present at a site. Sites with high organic enrichment (e.g., sewage effluent) may have a high density of invertebrates due to the increased source of food for many species. The actual number of individuals present in the to tal sample was not recorded; however, the number of grids in the sample tray that were searched to be st approximate the target number of 100 individuals was recorded. We used the number of grid cells search ed as a measure of the density of invertebrates present, or hyperabundance. Other measures of co mmunity structure were based on the number of individuals within specific taxonomic groups divided by the total number of individuals.


### Sensitivity and tolerance Beck (1954) developed a list of sensitive taxa for Florida that was used to calculate a biotic index, which was later modified to be the Florida Index. To ca lculate the Florida Index (which is more accurately termed a metric), very sensitive taxa are given a scor e of 2 and sensitive taxa a score of 1. The scores are summed to calculate this metric. We used the HDG to evaluate the sensitivity and tolerance of 1,195 taxa names. The taxa names included taxa that were not unique because the database included synonyms for the same taxa, as well as specimens identified to genus and species for the same genus. Because many sites had multiple visits and because less-disturbed (reference) sites tended to have a greater number of visits, we summarized data for each taxon by site to avoid bias associated with greater sampling effort at less-disturbed sites. We counted a taxon as present at a site if it was found for >= 50% of the site visits. We divided the 226 sites into 2 groups and, for simplicity’s sake, refer to these groups as “good” and “poor” for statistical testing. Good sites were defined as those with moderate levels of disturbance for no more than 2 measures (e.g., the LDI or NH 3 ) or high disturbance for no more than 1 measure (e.g., habitat condition). This translated into HDG <= 2 and included 141 sites. The remaining 85 sites were classified as poor. Thus, the 2 groups might be more accurately labeled better and worse, rather than good and poor. The relatively high number of sites selected to represent good condition reflects the large number of reference sites in the dataset. We used contingency table analysis to determine what percentage of taxon occurrences in the good-versus-poor range of HDG values represented a st atistically significant difference (chi-square test, Yates continuity correction). As for any test, the number of sampling points increases the power of theDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 12 test, such that less extreme difference will be statistica lly significant. For example, if a taxon occurred at 10 sites, all of the occurrences (100%) would have to be in the good range of the HDG to be statistically significant (p < 0.05). In contrast, if a taxon occurred at 38 sites, it would only need to occur at 30 of the good sites (79%) and would still be statistically significant. For this reason, we chose not to use a strictly statistical criterion for selecting sensitive taxa. Instead, for more common taxa we only selected thos e with 87% or more of their occurrences in good sites. Using this criterion of 87%, if a taxon occurred at 30 sites, 27 or more of the sites would have to be in the good range of the HDG. The p- value associated with this would be p < 0.002. A strictly statistical criterion would have selected taxa that prefer good conditions, rather than taxa that require good conditions. Thus, we used a statistical rule to define the minimum number of occurrences needed for statistical significance but made the st andards for selection more strict ( p << 0.05) for taxa that were more

common. Defining a statistical rule for the selection of to lerant taxa was not possible, because tolerant taxa can also “tolerate” good conditions. Instead, very tole rant taxa were defined as those found in at least 10 sites and for which >= 50% of their occurrences were in the poor range of the HDG. In some cases, multiple species within a genus failed to meet the cr iteria for inclusion as se nsitive (or very tolerant) because they were not present at enough sites. If several species within a genus (or family) were consistently found at good (or poor) sites, we comb ined the species into the parent genus (or family) and evaluated the genus (or family). For the BioRecon data, we only tested metrics used in the SCI in order to make the indexes as similar as possible. Relative abundance metrics coul d not be calculated for these data; therefore, only taxa richness metrics were evaluated. BioRecon metr ics were tested for correlation with the SCI and HDG.



## Index development and testing
We defined 2 indexes by selecting metrics that showed the most consistent response to the HDG. One index was developed for the SCI protocol ( 20 dip net samples and laboratory sorting of macroinvertebrates) and another for the BioRecon protocol (4 dip net samples and field sorting). For the SCI, we transformed metrics into unitless scores by determining the 95 th percentile values for each metric and then dividing each metric value by its range and multiplyin g by 10. Metrics that declined with disturbance were scored from 0 to 10, and metrics that increased with disturbance were scored from 10 to 0, so that the best biological condition was consistently scored as a 10. For skewed metrics, the natural log was calculated before di viding by the metric range. This process was done separately for the northeast, peninsula, and panhandl e regions. We tested for differences in metric expectation associated with different regions by co mparing the regression lines for each metric against the HDG and adjusted metric scoring as necessary to ensure similar responses for all metrics in the 3 regions. For the BioRecon Index, we scored metrics to match the SCI metric scores as closely as possible. Using histograms to ev aluate the overlap of the BioRecon and SCI metrics measured at the same sites, we adjusted metric scoring for the Bi oRecon metrics as needed. In order to distinguish between the BioRecon and SCI, BioRecon metrics were scor ed from 0 to 1 (rather than 0 to 10). In this way, the index values provide a quick clue as to which index is being reported. After correlation with disturbance was confirmed for both indexes, we evaluated different sources of variability, either due to natural sources (e.g., watershed size or season) or the sampling method (e.g., laboratory subsampling). We used correlation to test for dependence of the SCI on watershed size. To test the influence of season on the SCI, 78 sites with summer and winter samples were compared using a paired t- test. Summer was defined as May through October and winter as November through April. Within each season, data for repeat visits to each site were averaged.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 13 To evaluate other sources of variance, such as site differences, year differences, and repeat visits, we used an ANOVA model to estimate va riance components. For the SCI and BioRecon, we estimated variance associated with site, year, site x year interaction, and error. Error was defined as variance associated with repeat visits to the same si te within a year. For the SCI data, most of the repeat visits within a year occurred on the same day, rather than at different times during the year. For the BioRecon, most of the repeat visits were on different days within the same or a different year; very few were same day. Error estimates from ANOVA (mean squared error) were used to calculate the 90% confidence interval for both indexes and the number of categories of biological condition that each index can reliably detect (Zar, 1984). Confidence limits were calculated as:



### SCI 

## ⎟ ⎟ ⎠ ⎞ ⎜ ⎜ ⎝ ⎛ ± 645.1* 2



## n s

,

Where:

s 2 = error variance estimated from ANOVA (mean squared error), and n = number of samples taken from the site.

To calculate the number of categories each index could detect, we divided its range (0 to 100 for the SCI and 0 to 10 for the BioRecon) by the confidence interval. Other statistical approaches could be used to calculate the number of categories of biologic al condition that the indexes can reliably detect. For example, a 90% confidence interval could also be constructed based on the difference in SCI values for 2 sites. This approach would yield a sm aller confidence interval: instead of n = 1 in the equation above, n would equal 2 because 2 SCI values would be involved in calculating the difference. Currently there are no standards for which statistical model to use; ther efore, we used confidence intervals around the SCI for a single site because they are conceptually simple and somewhat conservative from a statistical point of view. In other words, this approach is less likely to be controversial (Johnson, 1999; Hoenig and Heisey, 2001). The number of categories of biologica l condition that an index could reliably detect were only used to summarize the precision of the indexes. The assessment categories used to define biocriteria for water quality standards were deriv ed from an entirely different process (see Chapter 4: Application of Biomonitoring Tools below). We also used an ANOVA model to estimate variability associated with laboratory subsampling. For this model, data from 59 sites with 3 subsamples each were used to estimate site variance and error variance. For this model, error was defined as the differences associated with subsamples from the same site visit. 

## Statistical model assumptions
The method described here for calculating confi dence intervals assumes that the multimetric indexes are normally distributed. Other studies have evaluated the statis tical properties of multimetric indexes and found that multimetric indexes for fish and invertebrates are unimodal and symmetric (Fore et al., 1994; Doberstein et al., 2000; Blocksom, 2003 ); thus, an underlying normal distribution is an appropriate assumption for multimetric indexes. A separat e and additional consideration is whether to use

z

-values from the normal distribution to calculate 90% confidence limits or values from Student’s t distribution. For small sample sizes, the t- distribution should be used for calculating confidence limitsDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 14 because variance may be underestimated; for large sample sizes (df > 30), however, the two distributions converge. We used data from 61 sites to estimate variance; therefore, z- values were appropriate. The 2 datasets used to estimate variance for the SCI and BioRecon Index were unbalanced—that is, the number of visits to each site varied for ea ch site. In contrast, the dataset used to evaluate laboratory subsampling was balanced with 3 subsamples for each site visit. ANOVA assumes a balanced design but is fairly robust to violations of this assumption (Milliken and Johnson, 1992). The primary consequence of an unbalanced design is related to the significance testing of model effects against the error term, which was not the purpose of this anal ysis. Rather, the goal for these ANOVAs was to estimate an error to use in the confidence interval ca lculations and to evaluate the relative contributions of year and error to the metrics and indexes. For estima tion of the error term, the large sample sizes mitigate any potential inaccuracy associated with u nbalanced cells. Sample sizes greater than about 30 are considered large, and this analysis had 61 sites for the SCI and 128 for the BioRecon.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 15 

# CHAPTER 2: RESULTS

Human disturbance gradient
The human disturbance gradient (HDG) was a consis tent predictor of biological condition for two independent rounds of testing. When metrics were r egressed against HDG for the two rounds of data, the slopes and intercepts of the regression lines were ne arly identical, indicating that measures included in the HDG yielded a reliable meas ure of human disturbance (Figure 1). Correlations among the four measures of human di sturbance used to defi ne the HDG were high, indicating good agreement among the different measures (see Table 4). All the individual measures, however, were more highly correlated with the HDG, suggesting that the HDG was a better overall measure of general human disturbance. The SCI showed a similar pattern and was more highly correlated with the HDG than the indivi dual measures of human disturbance.



# Metric selection
On average, 30 insect taxa were found in each sample for the 629 site visits used to test metrics. Diptera dominated the samples, with 23 taxa counted on average; of these taxa, about half were in the family Chironomidae. Of the remaining groups, 3 oligochaete, 2 Trichoptera, 1 Ephemeroptera, and 1 Plecoptera taxa were found, on average. For many metrics, the change in values from l east to most disturbed sites was dramatic. We compared the 20 best sites (HDG = 0 and the lowest values for LDI_WS) with the 18 worst sites (HDG > 6) and found that total taxa richness went from 33 to 19, on average. The number of Ephemeroptera taxa declined from 2 to 0, Trichoptera taxa from 4 to 0, clinger taxa from 8 to 0, and sensitive taxa from 9 to 0. The relative abundance of filterers declined from 22% to 3%, while the relative abundance of tolerant individuals increased from 5% to 69%. In general, we selected at least one metric from ea ch of the categories below according to which metric was most highly correlated with the HDG. If two metrics were calculated fr om the same set of taxa (redundant metrics), we only included one metric.


## Taxonomic richness
EPT taxa richness was most highly correlated with the HDG, followed by Trichoptera and Ephemeroptera taxa richness (see Table 6). Both taxonomic groups disappeared from samples with high levels of disturbance (Figure 2). Correlation between the HDG and Plecoptera taxa richness was somewhat lower, primarily because very few plecopterans were found in samples from the peninsula region. We selected Ephemeroptera and Trichoptera ta xa richness as separate metrics because they have the potential to respond to different types of di sturbance. We did not select Plecoptera taxa richness as a metric because it could only be applied in two of the three regions. Total taxa richness had the next highest correlation and was selected as an indicator of t he variety of taxa that a stream site could support.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 16 Figure 1. Number of EPT taxa and total number of taxa declined as human disturbance increased. Nearly identical regression lines for two independent sets of sites (rounds 1 and 2) indicated a consistent relationship between HDG and biological condition.Number of EPT taxa

0 2 4 6 8 10 0 5 10 15 20 25

Round 1 Round 2

Human disturbance gradient (HDG )Number of taxa

0 2 4 6 8 10 0 20 40 60Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 17 0 10 20 30 40 50 60Total number of taxa Median 25%-75% Non-Outlier Range Outliers

0 1 2 3 4 5 6 7Number of Eph. taxa 0 20 40 60% Filterer

-1 0 1 2 3 4 5 6Number of long-lived taxa

Figure 2. Six of the ten metrics included in the SCI plotted against the human disturbance gradient. All metrics declined with an increase in human disturbance. Metric values were averaged for each of the 176 sites. Samples with greater than 175 or less than 75 individuals were excluded before averaging. Number of sites within each box are shown ( N ). Plotted values are less than or equal to values on x-axis.

N = 80 41 25 8 16 6 0.8 2.4 4 5.6 7.2 Human disturbance gradient 0 2 4 6 8Number of Trichoptera taxa

0.8 2.4 4 5.6 7.2 Human disturbance gradient 0 5 10Number of clinger taxaDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 18 

## Feeding group

The relative abundance of filterer s (percentage filterer individuals) had the highest correlation with the HDG and the most consistent relationship with the HDG when graphed. Other feeding groups failed to show the consistent decline with disturbance that per centage filterer did. We also tested this metric without Cheumatopsyche (a somewhat tolerant genus of Trichoptera), but the changes in correlation were very small, and so all taxa were retained (Spearman’s r = –0.46 with Cheumatopsyche and –0.47 without).


## Voltinism
Correct information about generation times was not easily obtained for all Florida taxa; therefore, we only tested long-lived taxa richness and percent age long-lived individuals. Long-lived taxa included semivoltine insects and noninsects that require mo re than one year to complete their life cycles. Longlived taxa richness was more highly correlated with the HDG than was relative abundance of long-lived individuals; consequently, long-lived ta xa richness was included in the SCI.


## Habit
Clinger metrics measured either as taxa richness or as percentage of individuals were among the most highly correlated metrics with the HDG. We sele cted clinger taxa richness for inclusion in the SCI. 

## Community structure
Percentage dominance increased with the HDG and was included in the SCI (Figure 3) ; hyperabundance failed to correlate with the HDG. Although percentage Ephemeroptera and Trichoptera individuals (relative abundance) were both strongly co rrelated with the HDG, they were calculated from the same information used to calculate the taxa ric hness of those two groups. Because the taxa richness metrics were more highly correlated with the HDG , the relative abundance of Ephemeroptera and Trichoptera was not included in the index. Correlation for the relative abundance of Tanytarsini midges was not quite as high as the other composition metric s (–0.45), but was included in the SCI as the best available measure of the chironomid assemblage. 

## Sensitivity and tolerance
The number of taxa selected as sensitive equaled ~12% of the taxa tested, and the number of very tolerant taxa was ~10% of the taxa tested. Many sensitive taxa belonged to the Ephemeroptera or Trichoptera; several chironomids were also included (Appendix C). All the Plecoptera were included, though most of the genera and species in this group were too rare to test. Considered as a group, out of 191 occurrences of Plecoptera taxa, only one was found in the poor section of the HDG. All Plecoptera taxa showed a strong tendency to be found in the best of the good (minimally disturbed) sites. Many of the sensitive taxa selected by this study were pr eviously noted by Beck (1954) and used in the Florida Index, but several taxa were new to the list. Many of the taxa included on Beck’s list were too uncommon to test with this dataset.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 19 . 20 40 60 80% Dominance

0 5 10 15Number of sensitive taxa

Figure 3. Four of the ten metrics included in the SCI plotted against the human disturbance gradient. Percent dominance and very tolerant individuals increased with disturbance; sensitive and Tanytarsini midge taxa declined. Metric values were averaged for each of the 176 sites. Samples with greater than 175 or less than 75 individuals were excluded before averaging. Number of sites within each box are shown ( N ). 0.8 2.4 4 5.6 7.2 Human disturbance gradient 0 30 60 90% Very tolerant individuals

0.8 2.4 4 5.6 7.2 Human disturbance gradient 0 10 20 30 40% Tanytarsini

N = 80 41 25 8 16 6Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 20 The very tolerant list included one odonate ( Argia sedula ) and several chironomid taxa ( Larsia spp. , Cricotopus bicinctus, and Polypedilum illinoiense grp.) previously noted as sensitive and included in the Florida Index (Appendix D). The very tolerant list was dominated by noninsects and chironomids, along with several odonate taxa. One species of tubificid worm, Limnodrilus hoffmeisteri , was included as very tolerant, although it was present at 47% of the poor sites that fail ed to meet the criterion of > = 50% of poor sites. L. hoffmeisteri was included as very tolerant becaus e it was very common at all sites. It occurred at 70 out of 85 poor sites, and tended to in crease in relative abundance with disturbance. Other studies have also reported tolerance of L. hoffmeisteri to a variety of disturbance types (Chapman, 2001; Chapman et al., 1982) The total number of taxa with 10 or more occurrences was 168. When a large number of tests are conducted, questions arise regard ing the probability of Type I error. The detection of a statistically significant result will occur wi th the approximate frequency of p- value. For a p- value of 0.05 and 168 tests, approximately 8 taxa may be selected by chance alo ne (0.05 * 168 = 8.4). The number of sensitive taxa falsely selected from this analysis is likely much lower because the crit erion for selection was more strict than statistical significance. In other words, the p- values associated with 87% occurrence of more common taxa, e.g., taxa present at 20 or more sites, were much smaller.


## BioRecon metrics
The six taxa richness metrics calculated from BioRecon data were significantly correlated with the HDG and highly correlated with the SCI ( r ranged from 0.60–0.73; Table 7 ). The four SCI metrics calculated as percentages could not be tested because relative abundance was not recorded for BioRecon data. For this reason, we tested the very tolerant metric in terms of taxa richness rather than as a percentage. Its correlation was poor with the HDG and nonsignificant with the SCI, and therefore it was not included in the BioRecon Index.



# Multimetric index construction and evaluation

Ten metrics were selected for inclusion in the SCI, and six were selected for the BioRecon Index. Most of the SCI metrics required regional scoring ( Table 8; Appendix E; FDEP, 2007)

. In general, taxa richness tended to be higher in the panhandle region than in the peninsula or northeast. For the SCI, the highest correlation between metrics occurred between Trichoptera and clinger taxa (Spearman’s r = 0.77) and clinger and sensitive taxa ( r = 0.84). To determine whether these metrics were redundant, we evaluated the taxa included in each metric. Of the clinger taxa, approximately 41% were also sensitive taxa, and 33% were also Trichopt era taxa. Thus, we concluded that the statistical correlation was due to an underlying association with human disturbance, rather than redundant information. Two metrics were somewhat skewed, that is, their distributions had long ”tails” with sparse values at one end of their distribution. For these metrics, we calculated the nat ural log of metric values before dividing by the range of the metric values. In this way, metric scores were more evenly spread between 0 through 10. Two SCI metrics increased with disturba nce (% dominance and % very tolerant individuals); all others declined with disturbance. BioRecon metric scoring rules differed somewhat fr om SCI metric scoring. Taxa richness tended to be lower in two regions, while Ephemeroptera taxa richness was much higher in the panhandle (Table 9). More long-lived taxa tended to be found in all three regions for BioRecon samples.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 21 Table 7. Correlation of BioRecon metrics and th e old and new BioRecon Indexes with the HDG and SCI Metrics included in the BioRecon Index are noted (*). A ll correlations shown were statistically significant (Spearman’s r, p < 0.01, one-sided test, n = 116 for SC I, n = 53 for HDG). Data from multiple visits were averaged for each site.

Metric/Index HDG SCI BioRecon Index -0.58 0.75

Total taxa* -0.36 0.53 Ephemeroptera taxa* -0.47 0.62

Trichoptera taxa* -0.65 0.68 Long-lived taxa* -0.46 0.65

Clinger taxa* -0.48 0.67 Sensitive taxa* -0.63 0.69 Very tolerant taxa NS NS Old BioRecon Index -0.41 0.58 EPT -0.61 0.71 Florida Index (metric) -0.53 0.69

Table 8. SCI metric name and range of metric values used to assign a score of 0 (indicating degraded condition) to 10 (indicating minimal disturbance) by region Metric values higher or lower than the listed range were assigned a score of 0 or 10 as appropriate. Metric ranges listed in reverse order indicate metrics that increased (rather than declined) with disturbance. For two marked metrics (*), the natural log was calculated for the range of values before scoring.

SCI Metric Northeast Panhandle Peninsula
Total taxa 16–42 16–49 16–41 Ephemeroptera taxa 0–3.5 0–6 0–5

Trichoptera taxa 0–6.5 0–7 0–7
% Filterer 1–42 1–45 1–40 Long-lived taxa 0–3 0–5 0–4

Clinger taxa 0–9 0–15.5 0–8
% Dominance 54–10 43–10 54–10 % Tanytarsini * 0–26 0–26 0–26
Sensitive taxa 0–11 0–19 0–9 % Very tolerant * 78–0 36–0 59–0Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 22 Table 9. BioRecon metric name and range of metric values used to assign a score of 0 (indicating degraded condition) to 1 (indicating minimal disturbance) by region Metric values higher or lower than the listed range were assigned a score of 0 or 10 as appropriate. Underlined text indicates metric ranges that differed from those for the SCI.

BioRecon Metric Northeast Panhandle Peninsula
Total taxa 14–37 16–49 11–36 Ephemeroptera taxa 0–3.5 0–12 0–5

Trichoptera taxa 0–6.5 0–7 0–7 Long-lived taxa 0–6 0–10 0–7

Clinger taxa 0–7 0–15.5 0–8
Sensitive taxa 0–11 0–19 0–9

The SCI was highly correlated with the HDG, with minimal overlap between values for extremely disturbed and minimally disturbed sites (Figure 4). The BioRecon Index was also highly correlated with the HDG and was more responsive across the range of human disturbance than the original BioRecon Index (Figure 5). Regional scoring at the metric level resulted in an SCI that showed a similar response to human disturbance within each region. The regression lin es for the SCI versus HDG for the northeast and peninsula regions were nearly identical; the line fo r the panhandle region diverged somewhat from the other two regions for more degraded sites (Figure 6; Appendix F). The divergence was associated with two sites in the panhandle with SCI values that were higher than expected. We did not alter the metric scoring rules, because several other panhandle sites did obtain the lowest SCI values for high levels of disturbance. We calculated the natural log of the area upstr eam of the sampling site to test for correlation between the SCI and watershed size. Watershed size had an underlying correlation with the HDG (see Table 4) because the most disturbed sites tended to have small watersheds. To avoid this confounding association, we divided the sites into 3 groups (low, medium, and high disturbance) before testing for correlation between the SCI and watershed size. T he low disturbance group of sites had HDG values equal to 0. We defined medium disturbance as HDG values from 2 to 5 and high disturbance as HDG values > 5. The SCI was not significantly associated with watershed size for any of the 3 levels of disturbance ( Figure 7; Spearman’s r = –0.01, –0.005, 0.12, p > 0.5). The relationship between the SCI and HDG was consistent across years as well. When site visits were divided into 2-year segments for 10 years of sampling (1992–2001) and the SCI regressed against the HDG, the 5 regression li nes were nearly identical (Figure 8). This result indicates that the pattern of association between the SCI and HDG was independent of year. To verify that the SCI was correlated with the HDG, we used an independent set of 23 sites not used in any of the previous analys es. Correlation was high (Spearman’s r = –0.81, p < 0.001; Figure 9 ). Two sites with > 300 individuals in the samples had so mewhat higher SCI values than predicted by the HDG, possibly due to the inflation of taxa rich ness measures associated with large samples.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 23 

# Statistical precision of indexes
During the 2004-2006 period, the target number of individuals for laboratory identification for the SCI was 95 to 115. For the metric testing dataset, only 27% of the 629 site visits met this target. Most samples had well over 100 individuals. For these dat a, 32% of the samples had > 175 individuals, with some samples ranging up to hundreds of individuals. At the other end of the scale, only 36 samples (6%) had fewer than 90 individuals in the entire sample. When we compared small sample sizes with standard (or large) sample sizes from the same sites, samples with < 75 individuals contributed a di sproportionately large amount of variability to the repeat visits (Figure 10). SCI values were typically much lower fo r these small samples. For this reason, we excluded these 17 samples from the analysis of vari ability, which reduced the number of sites from 62 to 61. Repeat visits to the same sites were used to evaluate the sources of variability for the SCI and BioRecon. The sources of variance included differences as sociated with sites, years, visits within years, season (summer versus winter), and laboratory subsampling. From the ANOVA analysis, the variance associated with sites was greatest (304.9, 71%), the variance associated with year was relatively sm all (7.7, 2%), and the variance components associated with site x year interaction and error were similar (54.6, 12%, and 66.6, 15%; Figure 11; Appendix F ). For this model, error was defined as repeat visits with in years, about half of which were on the same day. We used the estimate of error variance from t he ANOVA to estimate the 90% confidence limits around the SCI as +/– 13.4 points, or a 27-point spre ad. Graphic representation of this range around the observed values for repeat visits captured all visits to the least disturbed sites (SCI values > 70), but failed to include all the visits to sites with lower SCI values (Figure 10). These sites graphically illustrate the site x year variance, that is, certain site-year co mbinations that changed in unique ways. The fact that undisturbed sites did not show this pattern, while disturbed sites did, suggests that the SCI was measuring real changes in biological condition, rather than “noise” associated with natural variability. Using the length of the 90% confidence interval (27 points) to divide the range of the SCI (0 to 100) yielded 3.7 categories of biological condition that SCI could reliably detect based on a single site visit. Variance components calculated for the SCI comp onent metrics showed similar patterns to the SCI. Site differences and site x year variance typically contributed the largest percentages to the overall variance (Figure 12). The exception was long-lived taxa rich ness, which had a large error component (with error defined as variance associated with repeat visits within years, as above). The least variable metrics were Ephemeroptera, clinger and sensitive ta xa richness, and % very tolerant. Overall, the SCI was less variable than most of its component metrics.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 24 Figure 4. SCI declined as human disturbance increased. Index values were averaged for each of the 176 sites. Only samples with 75-175 individuals are shown. Number of sites in each box are shown ( N ). 

### N = 79 42 25 7 17 6 0.8 2.4 4.0 5.6 7.2 

### Human disturbance gradient 0 20 40 60 80 100SCI Median 25%-75% Non-Outlier Range OutliersDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 25 Figure 5. Original BioRecon index based on three metrics (total taxa, EPT taxa, and Florida index; upper panel) and current BioRecon index based on six metrics (lower panel). Both indexes declined as human disturbance increased, but the 6-metric index has a broader range of response to disturbance. Values in boxes are <= value on x-axis. 

### N = 27 8 7 7 4 0 1 2 3Old BioRecon index



### 0135 Human disturbance gradient 0 2 4 6 8 10BioRecon index Median 25%-75% Range OutliersDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 26 Figure 6. SCI declined as human disturbance increased. Regression lines for the peninsula and northeast had nearly identical slopes indicating a similar index response across the gradient. Three visits to two sites with higher values than predicted (circled) increased the slope for panhandle sites somewhat. Index values were averaged for each of the 176 sites. Only samples with 75-175 individuals are shown and were included in the regression. N = 58 (NE), 66 (PS), and 52 (PH). 

### Human disturbance gradientSCI

02468 0 20 40 60 80 100

NE PS
PHDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 27 Figure 7. SCI was not significantly associated with watershed size (catchment area) when correlation was tested separately for low, moderate, and high levels of human disturbance (HDG). Highly disturbed sites tended to be small in catchment area and this relationship created a spurious correlation between SCI and catchment size. Shown are average SCI values for 176 sites. Actual catchment area shown below figures (in acres). 

# Ln(Catchment area)SCI



### 5 7 9 11 13 15 0 20 40 60 80 100

5 7 9 11 13 15 5 7 9 11 13 15 

## HDG: <=1 (1, 5] > 5440,00060,00081001100150440,00060,00081001100150440,00060,00081001100150Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 28 Figure 8. SCI showed a similar response to human disturbance for different years. Shown are five regression lines representing data from 1992-3, 1994-5, 1996-7, 1998-9, and 2000-1. The nearly identical slope lines indicate a consistent relationship between SCI and human disturbance for different time periods. 

### Human disturbance gradientSCI

0 2 4 6 8 10 0 20 40 60 80 100Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 29 Figure 9. Correlation between SCI and the human disturbance gradient was high for an independent test of 23 sites not included in any previous analyses (Spearman’s r = -0.81, p<<0.01). Four sites were missing data for NH3 (open circles) and two sites had >300 individuals (open triangles). 

### 012345678 

## Human disturbance gradient 0 20 40 60 80 100SCIDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 30 Figure 10. SCI values for repeat visits to 62 sites are shown. Sites were first sorted according to SCI value. Individual site names are not shown, but all SCI values for each site are plotted at a single point on the x-axis. Thus, vertical lines of points represent the range of SCI values at each site. Samples with < 75 individuals are indicated by solid points (upper panel). Lines (lower panel) represent the +/- 15 point confidence interval for the SCI when small samples were eliminated. 0 20 40 60 80 100SCI



### Site

0 20 40 60 80 100SCIDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 31 Figure 11. Estimate of variance components and their relative percentage for SCI. The largest percentage of variance was associated with site differences. Replicates collected within a single year (primarily on the same day) accounted for approximately 15% of the total observed variance. Year differences accounted for 4% of the variance. The interaction of site and year accounted for 16% of the total variance. 

### Error, 66.6, 15% S x Y, 54.6, 12%

Year, 7.7, 2%

Site, 309.9, 71% 

## SCI Variance ComponentsDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 32 Figure 12. Variance components for SCI and its metrics. For the index and several of the metrics, variance associated with site differences represented the largest component of variance. Error variance associated with repeat visits within years (primarily on the same day) was highest for long-lived taxa richness. The least variable metrics were Ephemeroptera and sensitive taxa richness, and percent very tolerant individuals. 

## Error Site x Yr Year SiteSCITotal taxaEph. taxaTri. taxa% FiltererLong-livedClinger% Dom.% Tany.Sensitive% Toler. 

## 0 20 40 60 80 100% Relative varianceDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 33 For the seasonal comparison, sites were chosen to represent locations with minimal human disturbance, in order to eliminate this potentially confounding source of variability from the analysis. Unfortunately, over half the SCI values for these site visits were < 70 (the “exceptional” category), indicating the seasonal dataset represented a broad range of conditions. Highly degraded sites (SCI < 20) were not included in the seasonality analysis. SCI values were on average 3.5 points higher in winter than summer (paired t- test, p = 0.049). The observed difference was greater in the northeast (6.1 points) than the panhandle (1.6 points) or peninsula (3.6 points; Figure 13 ). Using the same data, we evaluated metrics to det ermine which were contributing to the seasonal differences in the SCI. Five metrics had values in t he winter indicating better biological condition; these were Ephemeroptera taxa richness, % filterers, % T anytarsini, sensitive taxa richness, and % very tolerant. Long-lived taxa richness had higher values in the summer, the opposite of the SCI. Although significant, most metric differences were rather small compared with the overall range of the metric (Table 10). The paired t- test is a very powerful test and can detect very small differences because it compares each site with itself. The exception was % Tanytars ini. With a difference of 4.6% compared with a possible range of 0 to 26%, it had the largest relative change from summer to winter. We did not modify metric scoring to correct for seasonal differences, because most metric differences were very small and the difference between SCI summer and winter values was small (3.5%) relative to the site differences through time. To evaluate the influence of laboratory subsam pling on the SCI, we used data that included multiple subsamples from the same site visit. We es timated variance using ANOVA with stream site-visit (n = 59) as a single factor and the 3 subsamples from each visit to estimate the error variance. Error variance for this model was 32.86, which represent s about half the error variance calculated above when error was defined as differences associated with repeat visits to the same site (32.86/66.6 * 100 = 49%). In other words, of the variance asso ciated with repeat visits to the sa me site, about half the variability is due to laboratory subsampling. However, because the variance estimates were from different datasets, the comparison should be cautiously applied. After these variance estimates for this report were completed, laboratory subsampling methods were tested and modified to address this variability. (See results and discussion in Appendix G .) The BioRecon Index was calculated as the sum of the 6 scored metrics; therefore, the sum of the scores ranged from 0 to 6. We transformed the inde x to a range of 0 to 10 by dividing the sum by 6. The dataset for this ANOVA model was unbalanced, with many site-year combinations missing; therefore, the site x year interaction component of variance coul d not be estimated. The components of variance were similar to those for the SCI, in that the largest com ponent of variance was associated with site differences and year variance was again small (Figure 14). The error variance for this model was 1.46, with error derived from repeat visits to the same sites. A 90% confidence interval for the BioRecon Index yielded a limit of +/- 2, or a length of 4.0 poi nts. Dividing the range of the BioRec on index (0–10) by 4 translated into 2.5 categories of biological condition that the BioRecon could statistically distinguish

. This result supported the decision to identify three assessment categories for the BioRecon index in a regulatory context (see Chapter 4: Application of Biomonitoring Tools below).Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 34 Figure 13. SCI values by region (northeast, peninsula, and panhandle) and season. Summer samples (circles in center of box) tended to be lower than winter samples (diamond centers) in the northeast and peninsula regions. N = 23 (NE), 34 (PS) and 31 (PH). 

## ne ps ph 0 20 40 60 80 100SCIDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 35 Figure 14. Variance components for BioRecon and its component taxa richness metrics. Variance associated with site differences represented the largest component for metrics and index. Error variance associated with repeat visits within years was similar across metrics ranging from 20-30% of the total. Variability associated with a specific year was relatively small for the index and metrics. 

## Erro r Year SiteBioReconTotalEphem.Trichop.Long-livedClingerSensitive 

### 0 20 40 60 80 100% Relative varianceDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 36 Table 10. Results for seasonal comparison of the SCI and its component metrics Differences between summer and winter samples (negat ive value indicates higher winter values). Significant differences noted (* p < 0.05, paired t-test).

Index/metric Difference SCI –3.5 *
Total taxa 0.1 Ephemeroptera taxa –0.4 *

Trichoptera taxa 0.3 % Filterer –5.2 * Long-lived taxa 0.6 *

Clinger taxa –0.2 % Dominance –1.9 % Tanytarsini –4.6 * Sensitive taxa –0.9 * % Very tolerant 2.0 *

These categories for the BioRecon may be somewhat conservative, particularly for sites with good biological condition, because the variability estimated from repeat visits was much higher for moderately disturbed sites than fo r sites with minimal disturbance (Figure 15). If variance were estimated using only minimally disturbed reference sites, the er ror variance would be smaller. To determine whether high variability was associated with natural variabilit y or changes in human activities, we selected the sites with the most variable BioRecon values thr ough time and asked regional biologists to note any change in human activities at the site (Table 11). Four of the sites improved where roads had been paved to eliminate sediment runoff. Other changes were asso ciated with pesticide spraying, the elimination of runoff from a wastewater treatment plant, road closure, and the cessation of fertilization upstream. The results from this anecdotal analysis suggest that mu ch of the BioRecon variability was associated with real changes in human disturbance, rather than natural variability. Variance results for the BioRecon components metr ics were similar to those for the SCI metrics, with site differences contributing the largest component of the total variance (see Figure 14). In general, the metric variance due to nuisance sources of variab ility (year or error) was smaller for three of the BioRecon metrics than for the same SCI metrics. Lowe r error variance for total taxa, Trichoptera, and long-lived taxa richness suggests that the BioRecon protocol may provide more reliable estimates of these metrics. Direct comparisons of the results from different datasets should be cautiously applied, particularly for relative variance estimates, because they depend on the magnitude of the site differences.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 37 Table 11. STORET site code, region, station nickname, observed change in BioRecon values through time, and possible reason for change Listed sites represent locations with the highe st variability observed for BioRecon values.

STORET Region Station Change Reason
20010521 Peninsula JIMREF Declined Unknown
20010525 Peninsula LILHAW@40 Declined Unknown 20030341 Northeast CECFLD7 Improved Unknown
32030024 Panhandle SFBEARREF Improved Intermittent pesticide spraying

33020064 Panhandle PBRNCSTLR Improved Section 319 nonpoint source (NPS) restoration project to pave a road and stop sediment

33020065 Panhandle BRSTWRKB Improved Section 319 NPS restoration project to pave a road and stop sediment 33020067 Panhandle CNOECPBRNR Improved Section 319 NPS restoration project to pave a road and stop sediment

33020082 Panhandle SANHOLTST Improved Section 319 NPS restoration project to pave a road and stop sediment

33030039 Panhandle TRKLNHDCM Improved Partnership with state forest and the Nature Conservancy closed logging road and eliminated sediment 33030102 Panhandle TURKEYCR Improved Summer aerial pesticide spraying
33040016 Panhandle WILLIAMTST Improved Runoff from wastewater treatment plant sprayfield eliminated

33040017 Panhandle DDFLCWC189 Improved Ended fertilization upstream of sites in state forest 24030013 Peninsula HILSTP4REF Improved Unknown
22020062 Panhandle OKLREF Variable Unknown
27010050 Peninsula MOSESUS1 Variable Unknown
27010583 Peninsula LITLTOMOKA Variable UnknownDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 38 Figure 15. Range of BioRecon index values for repeat visits to 128 sites. Site names are not shown; however, each vertical line represents a single site with the minimum and maximum index values plotted as the endpoints. Note that high and low index values were less variable for repeat visits through time. 

### Site 0 2 4 6 8 10BioRecon indexDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 39 

# CHAPTER 3: DISCUSSION

The SCI and BioRecon Index, along with their com ponent metrics, were developed to assess the biological condition of stream sites using samples fr om the stream macroinvertebrate assemblage. These assessment tools will be used primarily to define biologica l criteria for freshwater streams and to evaluate the effectiveness of best management practices (BMPs) (Vowell, 2001). Three results support the use of the SCI for this purpose. First, the component metr ics showed a strong and consistent response to an independent measure of human distur bance (HDG). The SCI was also highly correlated with the HDG using an independent dataset for verification. Second, the SCI was independent of watershed size and geographic region. This result means that the SCI c an be used across the state to compare stream sites. Third, the influence of seasonal and annual differences on the SCI was relatively small. Index values were about 3.5% higher for winter samples, and about 2% of the total variance of the SCI was associated with year of sampling. Although low, these sources of variance should continue to be evaluated with more complete sampling designs because the influence of both could potentially be eliminated by adjusting index values according to season or year.


# Human disturbance gradient
The HDG simplified several aspects of metric and i ndex testing. First, it provided a more stringent test of metric association with disturbance than a co mparison of reference versus test sites. Percentage Diptera individuals serves as an example of this. Th is metric was included in the old SCI because it increased at disturbed sites; however, across a gradient of disturbance, it failed to consistently respond. Second, the HDG was independent of biological condit ion because no measures of biological condition were included. Therefore, metric expectations could be defined for equivalent levels of disturbance within each region. As a consequence, it was not necessary to correct the multimetric index for regional differences. Third, objective selection criteria based on association with the HDG were used to define individual taxa as sensitive or very tolerant. Fina lly, the HDG resolved a spurious correlation between watershed size and the SCI. During initial analysis, the SCI appeared to be correlated with watershed size because the most disturbed sites happened to be very small. After controlling for the HDG, the correlation between watershed size and the SCI was e liminated. The HDG can also be used to describe the intensity of disturbance associ ated with specific biological losses, as measured by the SCI and its component metrics.


# Biological indicators

Multimetric indexes strive to integrate measures from a diverse set of biological categories for two reasons. First, monitoring different aspects of t he biological assemblage improves the likelihood of detecting changes associated with different types of disturbance. Second, the potential exists to define metric signatures, that is, associations between specif ic metrics (or suites of metrics) that correspond to specific human activities (Norton et al., 2000; Yoder and DeShon, 2002). Metric signatures are particularly relevant to the TMDL process, which allocates responsibility for degraded stream condition among the various human activities in the watershed (Karr and Yoder, 2004; EPA, 2000). Though not specific to any particular group, tota l taxa richness represents a general measure of the biological complexity found at a site. This metric is one of the most widely applied in biomonitoring programs because of its consistent decline with hum an disturbance for stream invertebrates (Kerans and Karr, 1994; Fore et al., 1996; Karr and Chu, 1999; Klemm et al., 2002), as well as fish (Hughes et al, 1998), terrestrial invertebrates (Kimberling et al ., 2001), and birds (Bryce et al., 2002). Ephemeroptera and Trichoptera taxa richness have also been widely applied, though often combined with Plecoptera into a single (EPT) metric. Splitting these taxonomic group s provides the opportunity for metric signaturesDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 40 associated with different types of disturbance. For example, E phemeroptera taxa are known to be particularly sensitive to metals and will disappear before other taxa (Clements et al., 2000; Fore, 2002). On the other hand, an increase in Ephemeroptera ta xa may indicate increased nutrients (Miltner and Rankin, 1998). Filterers are expected to decline in re sponse to disturbance because of the increase in sediment and silt that can damage or clog nets. Long-lived taxa are expected to decline as human disturbance alters the natural flow regime, because these taxa require water in the channel year-round. Pollution events of short duration may also eliminate these taxa, while other taxa may colonize from unaffected sites. Clinger taxa have morphological and behavioral adaptati ons that allow them to cling in fast water. Human development near stream sites in Florida o ften translates into eroding sand that can smother habitat and eliminate these taxa. Other studies have found these taxa to be sensitive to disturbance associated with mining and urbanization (Fore et al., 2001; Mebane, 2002). Percentage dominance of the most abundant taxon increases with disturbance as the natural taxonomic diversity declines and very tolerant taxa dominate samples. This metric repr esents a measure of the ov erall structure of the assemblage and has been associated with disturbance in several regions (Klemm et al., 2002). The Tanytarsini midges are used as indicators by Oh io because of their general sensitivity to human disturbance (DeShon, 1995). Three Tanytarsini midge ta xa were also selected for inclusion in the list of sensitive taxa for this study. Several of the sensitive taxa agreed with Beck’s ( 1954) designations used to calculate the Florida Index; however, a few reversals were noted for taxa that satisfied the criteria for very tolerant. The primary difference between the sensitive taxa list and the Florida Index list was the exclusion of many rare taxa that could not be tested. The list of sensitive taxa is now shorter; nonetheless, the objective criteria used to select these taxa make these me trics easier to justify in a regulatory context. The set of metrics selected for Florida repres ents a convergence with similar studies and programs in other states (Karr, 1998). Many of t he Florida metrics also had a strong correlation with disturbance in Colorado, Idaho, Washington, Tennessee, and Japan, where they responded to a diverse set of human activities including timber harvest (Fore et al., 1996), recreation (Karr, 1998), urbanization (Fore et al., 2001; Karr and Morishita Rossano, 2001; Morley and Karr, 2002), agriculture (Kerans and Karr, 1994), and mining (Mebane, 2001; Fore, 2002; M ebane, 2002). The emergence of a core set of metrics across a variety of geographic contexts furt her supports the use of these metrics as biological indicators.



# Sources of variance

We evaluated five sources of nuisance variance for the SCI and two for the BioRecon Index. For the SCI, we estimated variance associated with laboratory subsampling, repeat visits within a year (error), different seasons, different years, and site x year interaction. For the BioRecon, we estimated variance due to repeat visits within a y ear (error) and different years. One of the largest contributors to the difference s observed for the SCI values collected from the same site within the same year was the variability associated with laboratory subsampling. Subsampling represented approximately 49% of the variance due to repeat visits to a site within the same year. Unfortunately, the two variance estimates were deri ved from different datasets; thus, this percentage should be interpreted with caution. Nonetheless, subsampling in the laboratory seems to be a significant source of variability. Furthermore, the subsampli ng method for this small study was more carefully controlled than for routine sampling, in that the target of 100 individuals per sample that was in use at the time of this study was more frequently attained. Thus, the subsampling analysis did not include any additional variability associated with variable sample sizes; consequently, variance due to subsampling may actually be higher for routine samples. (See Appendix G for analysis and changes made to the subsampling protocol after the evaluation of source s of variance was completed for this document.)Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 41 Site by year interaction represents the vari ability associated with some unique site-year combinations. This source of variance represented in dex values from sites that increased (or decreased) from one year to the next in a different or more ex treme manner than did other sites during those same years. The differences could be due to natural variability , e.g., the effect of a dry year on some sites but not others, or actual changes in biological condition associated with human influence. These two sources could not be distinguished using the current data, because a ll sources of human disturbance were not quantified. For example, agriculture is a dominant land use in Florida. Pesticide treatments represent a potentially significant impact to stream invert ebrates but could not be evaluated for this study. Nonetheless, the higher variability in both the SCI and BioRecon Index values observed for sites with moderate index values compared with sites with high index values suggests that both indexes may be measuring real changes associated with human land use, rather than nuisance variance. Other studies have found a similar pattern of increased variance asso ciated with greater human disturbance (Karr et al., 1987; Steedman, 1988). For the BioRecon Index, variab ility for several sites was explained by changes in human land use. Road paving to eliminate erosion, th e elimination of toxic runoff, and the discontinuation of fertilization were all associat ed with improved BioRecon values. For the SCI, winter index values were significant ly higher than summer values collected from the same sites; however, the difference was small (3.5%). We did not adjust metric scores to eliminate the seasonal difference in the SCI because individual metric differences were relatively small and the differences in SCI values was due to a cumulative effect across several metrics. The seasonal difference was also relatively small compared with the variance d ue to other sources, such as repeat visits within a year or laboratory subsampling. The influence of seasonality awaits a better dataset that compares index values by season for sites with little or no change in human disturbance. For the BioRecon Index, the variance associated wi th repeat visits within years was somewhat higher than the variance observed for the SCI. This is largely because the site x year interaction term could not be estimated (due to incomplete data), and this variance was included in the error term. In addition, the BioRecon data included mostly samples fr om different days within the year, rather than the predominantly same-day samples used for the SCI. As a consequence, the variance estimate for the BioRecon Index may be somewhat high and the precision of the index underestimated. The higher variability noted fo r the BioRecon may be due to hum an disturbance at the site, different levels of sampling effort applied by field biol ogists, the smaller number of metrics in the index (6 for the BioRecon versus 10 for the SCI), or some comb ination. In the field, biologists examine the sample until all unique taxa have been identified. They also bring back examples of each taxon for laboratory verification. Biologists may vary both in the amount of time they spend searching for unique taxa and in the number of taxa returned to the lab. The protoc ol specifies that a minimum of 5 examples of each unique taxon be returned for verification. If multiple examples of each taxon ar e returned, the probability of identifying additional taxa increases as a functi on of the number of individuals returned. This is because many field identifications can only be made to the genus level, while in the lab more taxa can be identified to the species level. The precision of this index could likely be improved by applying the same level of effort to each sample in the field. In addi tion, the number of individuals returned to the laboratory for verification should be made consistent for each sample. (Note: these issues have been addressed in the current version of the BioRecon SOP.)Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 42 

# CHAPTER 4: APPLICATION OF BIOMONITORING TOOLS

This section takes the results from the previous chapters and describes how the biological monitoring tools can be integrated into a regulat ory framework for managing streams under the Clean Water Act. States are required to report to Congress the water bodies that are failing to support their designated uses. FDEP intends to use biological criter ia along with physical and chemical criteria to determine whether stream sites are meeting their desi gnated uses, particularly uses related to aquatic life use support. In this section the regulatory framewor k of the Clean Water Act is summarized; an approach for defining more specific designated uses for aquatic life use is described; and biological criteria based on the SCI and BioRecon Index are proposed. Two approaches were used to define categorie s of biological condition. The primary approach involved the U.S. EPA’s Biological Condition Gradient, which we used as a framework for defining biological standards in a regulatory context (EPA, 2005; Davies and Jackson, 2006) . A secondary, but parallel approach, adopted by many states, defines biological standards based on measurements, such as SCI, collected from a set of reference sites (EPA, 2006a, 2006b). We examined the reference site data and the variability associated with the SCI determinati on as a secondary line of evidence to support the impairment threshold decision. Additionally, the refe rence site data was used as the primary basis for proposing the biological criteria threshold for site s that may be considered of “exceptional” quality. 

# Regulatory framework
The regulatory context for the management of surfac e waters is derived from the mandates of the Clean Water Act (CWA; EPA, 2005; Davies and Jackson, 20 06). Within this framewor k, states and tribes must adopt water quality standards to protect their waters. Water quality standards (WQS) include three parts: 1) designated uses, 2) numeric and narrative crit eria that protect the uses, and 3) antidegradation policies to prevent deterioration of high-quality waters (EPA, 2006a). The CWA authorizes the U.S. EPA to determine appropriate minimum levels of protecti on and provide national oversight to state programs; however, considerable flexibility and discretion are left to states and tribes to design their own programs and establish levels of protection beyond the national minimums (EPA, 2005). WQS are part of state law and define the water quality goals for a water body by designating the use(s) and setting criteria necessary to protect the use(s). Thus, individual state WQS provide the foundati on for the management and protection of surface waters and pollution control programs. WQS prov ide the basis for determining attainment or nonattainment of water bodies in order to identify im paired waters and prioritize water bodies for TMDL (Total Maximum Daily Load) establishment. Historically , states and tribes have taken different approaches to defining their WQS. Different approaches are acceptable to EPA as long as a minimum standard is protected. In other words, states are encouraged to be more protective than the national minimum. Once WQS are in place, states are authorized to implement monitoring programs that allow them to report on the attainment of those standards and to identif y and prioritize waters not attaining standards. 

## Designated uses
All of the water quality protections availabl e under the CWA follow from the water body’s designated use. All state water bodies have desig nated uses as specified in water quality standards, whether or not the uses are currently being attai ned. When classifying water bodies and determining designated uses, a state must take into considerati on public water supplies, protection and propagation of fish, shellfish, and wildlife, recreation in and on the water, and agricultural, industrial, and other purposes, including navigation. The language of the CWA incl udes a general mandate for protecting waters forDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 43 fishing and swimming and the protection and propagation of aquatic life. States differ in their approaches to defining designated uses under this general mandate for the protection of aquatic life. Some states designate a general “aquatic life use” or have narrative biological criteria, which is often a general statement such as “aquatic life communities shall be maintained similar to aquatic life as naturally occurs”. Florida’s current description for Cl ass III waters provides an example of this approach: “recreation and propagation and maintenance of a heal thy, well-balanced population of fish and wildlife”. For states with a general narrative biological criterion, single thresholds are typically set based on a multimetric index of biological integrity, with expe ctations determined according to regional reference conditions (U.S. EPA, 2006a; Stoddard et al., 2006) . Water bodies are then assessed to determine whether they support or fail to support a single general use for aquatic life. Currently in Florida, FDEP has convened a Designated Uses Policy Advisory Committee (DUPAC) to explore an alternative system of designated use classifi cation for Florida. The DUPAC is considering the environmental, social, and economic co nsequences of use designation, and includes the interested public, the regulated community, water quality managers, and scientists. The scheme currently proposed through the DUPAC involves the separation of human and aquatic life uses and recommends a tiered system of aquatic life uses. To be consistent with the anticipated direction of the FDEP DUPAC, this chapter proposes three categories for the SCI. Subject to future rule making decisions, those three categories may be conceptually interpreted as “exceptional” (Category 1), “healthy” (Category 2), and “impaired” (Category 3). In this way, exceptional stream resources are id entified to support the intent of the anti-degradation component of the water quality standards (see below). Additionally, the “impaired” category supports decisions needed by other regulatory pr ograms, such as the TMDL program. 

## Water quality criteria
Water quality criteria should be closely tied to a specific designated use and should provide a meaningful assessment of whether the use is support ed. The assumption is that when criteria are met, the designated use is protected. Water quality criter ia may be either narrative statements that describe the conditions needed for a water body to achieve its designated use, or numeric levels of pollutants or chemicals that should not be exceeded so as to pr otect the designated use. While all States have adopted narrative criteria for the protection of aquatic life, only some have adopted numeric criteria such as constituent concentration levels for various polluta nts or specific biological index values. Biocriteria refer specifically to criteria based on biological as pects of the water body, typically measures of fish, macroinvertebrates, plants or algae. 

## Anti-degradation policy
This component of the WQS has traditionally received less attention than the other two components. An anti-degradation policy is required to ensure 1) water quality is sufficient to support existing uses; 2) where water quality is better than th at needed to support aquatic life uses, that level of quality must be protected unless a public process determines that quality can be lowered to allow economic or social development; and 3) the water quality of water bodies with exceptional recreational or ecological significance must be maintained and protected (EPA, 1985). Thus, states are required to provide a framework for managing water bodies when their water quality changes. It is anticipated the DUPAC will establish an anti-degradation policy to continue to maintain the water quality and biological health of exceptional water bodies.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 44 

# Tiered aquatic life uses

In 2001, the National Resear ch Council published a report on Assessing the TMDL Approach to Water Quality Management in which the authors recommended tiering designated uses for improving the decision-making related to setting water quality st andards (NRC, 2001). The NRC found the CWA’s goals to be too broad to provide the operational definition of designated uses needed to support aquatic life, and recommended greater specificity in defining aquatic lif e uses. For example, rather than stating that a water body needs to be “fishable,” the designated us e should specifically describe the expected fish assemblage (e.g., cold water fishery, warm wa ter fishery, or salmon, trout, bass, etc.). Tiered aquatic life

uses (TALUs) are bioassessment-based statements of expected biological condition in specific water bodies that allow more precise and measurable definitions of designated aquatic life uses (EPA, 2005). Designated uses are written in qualitative, narrative terms; therefore, the challenge is to relate a water quality criterion to the designated use. Establishing this relationship is more straightforward when the water quality measure, or criterion, is closely and meaningfully related to the designated use. For this reason, the NRC recommended the use of biological info rmation to define more appropriate aquatic life uses. The TALU approach provides an interpretative framework for developing a technical program that will tighten the linkage between narrative use st atements and numeric biological criteria. Designated aquatic life uses represent a state’s biological goals for their water bodies. Tiered aquatic life uses rely on biological information to mo re precisely define these goals relative to natural conditions. Bioassessments can then be used to measure attainment of the goals (EPA, 2005). Thus, the TALU approach is designed to support the implementat ion of biocriteria, and the scientific knowledge of aquatic ecology that they represent, into state and tr ibal water quality standards. The driving motivation here is to ensure that listings of impaired waters ar e scientifically defensible and to identify and protect high quality water resources. 

## Determining the impairment threshol d using the Biological Condition Gradient model
The objective of the Clean Water Act is to “restore and maintain the physical, chemical and biological integrity of the Nation’s waters.” To achieve this, a consistent, uniform interpretation of biological condition must be applied to data deriv ed from any assessment methodology (Davies and Jackson, 2006). This inte rpretive framework must define ecologi cal condition, allow for variability exhibited in the reference condition, and take into account the designated use of a given waterbody. The U.S. EPA has outlined a tiered system of aquatic lif e use designation, along a Biological Condition Gradient (BCG), that illustrates how ecological attri butes change in response to increasing levels of human disturbance. The BCG is a conceptual model that assigns the relative health of aquatic communities into one of six categories. The BCG is based on fundamental ecological principles and has been extensively tested and verified by aquatic biologists throughout the U.S. The BCG utilizes ten biological attributes of aquatic systems which respond predictably to increasing pollution and human disturbance. While these ten attributes are measurable, some are not routinely quantified in monitoring programs (e.g., ra te measurements such as productivity), but may be inferred via the community composition data (e.g., abunda nce of taxa indicative of organic enrichment). The attributes are:

I. Historically documented, sensitive, long-lived or regionally endemic taxa
II. Sensitive and rare taxa III. Sensitive but ubiquitous taxa
IV. Taxa of intermediate toleranceDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 45 V. Tolerant taxa
VI. Non-native taxa VII. Organism condition VIII. Ecosystem functions

IX. Spatial and temporal ext ent of detrimental effects X. Ecosystem connectance. The gradient represented by the BCG is divided into six levels (tiers) of condition that were defined during a series of national workshops with experienced aquatic biologists from across the U.S. (Davies and Jackson, 2006). The six tiers are described as:

1. Natural or native condition 2. Minimal changes in structure of the biotic community and minimal changes in ecosystem function 3. Evident changes in structure of the biotic community and minimal changes in ecosystem function 4. Moderate changes in structure of the biotic community with minimal changes in ecosystem
function 5. Major changes in structure of the biotic comm unity and moderate changes in ecosystem function 6. Severe changes in structure of the biotic community and major loss of ecosystem function.

Davies and Jackson (2006) recommend that regional biological experts adapt the conceptual tiers described by the BCG to local conditions using data from a regional monitoring program. The tiers can then be used in assessment and biocriteria. To dev elop a BCG for Florida streams, a panel of 22 experienced aquatic biologists were convened as part of a workshop. Participants were asked to apply and calibrate the general BCG model to stream data collected from Florida streams (Table 12; Appendix H) . Results from the workshop were used to define biological thresholds for the SCI. The panel of experts was provided with macroi nvertebrate taxonomic lists and biological metrics for 15 reference stream sites and 30 test samples, equally divided into Florida’s three bioregions (Panhandle, Northeast, and Peninsula). Data collectio n and metric calculation followed standard FDEP protocols. The panel was NOT given the SCI value asso ciated with any of these sites, or any physical, chemical, or habitat descriptions of the sites (other than the Bioregion location). Detailed descriptions of the BCG attributes and tiers were provided to the panel prior to the meeting. On the day of the meeting, a presentation reiterated these definitions, accompanied by extensive discussion (Frydenborg and Miller, 2007). The panel of 22 Florida scientists assigned the 30 test samples to BCG Tiers 1 through 6, generating a total of 660 individual sample assessmen ts. After discussion, there was general agreement that it was difficult to identify Tier 1 sites (pristi ne, natural condition). This was primarily due to inadequate information in the data sets to properly characterize BCG Attribute 1, “ Historically documented, sensitive, long-lived or regionally endemic taxa.” The 20 dip net sweep sampling method, along with the laboratory sub-sampling procedure, do not result in a complete bi ological census of any given site. This lack of data on rare, endemic taxa made it difficult for the gr oup to judge a “pristine” condition. Even given this constraint, there were approximately twenty instances where panelists assigned a score of Tier 1 to a sample, although none of the samples was unanimously assigned to Tier 1 by all 22 experts. However, there was complete agreement among the panelists that Tier 2 represented the minimally disturbed reference condition, which was considered, for practi cal purposes, to be almost indistinguishable from “natural.”Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 46 Table 12. BCG Workshop Participants Name of regional expert, affiliation, and years of experience in benthic macroinvertebrate identification,

Expert name Affiliation Years Jerrell Daigle DSA, Inc. >20
Dana Denson FDEP Central District 10-15 Doug Durbin Biological Research Associates >20
John Epler John Epler Consulting >20 Julie Espy FDEP Tallahassee 5-10
David Evans Water and Air Research >20 Michael Heyn FDEP Tallahassee >20 Joy Jackson FDEP Tallahassee 10-15 Deron Lawrence Biological Research Associates 10-15
Laura Line Water and Air Research 10-15 Rob Mattson St. Johns River Water Management District >20 Peggy Morgan FDEP Southwest District >20
Patrick O'Connor FDEP Northeast District >20
Manuel Pescador Florida Agricultural and Mechanical University >20 Marianne Pluchino Reedy Creek Improvement District >20
Eric Pluchino FDEP Orlando District >20 Donald Ray FDEP Northwest District >20 Johnny Richardson Leon County 10-15
Gitta Schmitt FDEP Southwest District 5-10 Mary Szafraniec Southwest Florida Water Management District 5-10
Albert Walton FDEP South District >20 Gary Warren Florida Fish and Wildlife Commission >20

During the workshop, the 22 panelists BCG scores were tabulated for each test sample and the panel discussed the results. In all cases, the majority of panelists’ scores were assigned to the same tier, with a small number of the adjacent tiers represent ed. After discussion on specifics associated with the data set, the panelists were given the o pportunity to modify their score. During the national work shops sponsored by EPA, different groups of exper ts independently concurred that the ecological characteristics describe d by Tiers 1–4 corresponded to how they interpret the Clean Water Act (CWA)’s interim goal for protec tion and propagation of aquatic life. They further identified Tiers 1 and 2 as indicative of biological integrity (U.S. EPA, 2005). Subsequent to these national workshops, several states have adopted a line of impairment near the threshold between Tiers 4 and 5 (U.S. EPA, 2006b). The Florida panel of experts was similarly asked to offer their opinion as to where along the 6 tiered rankings they believed the interim goal of the Clean Water Act (CWA) was met. In other words, sites below a selected tier threshold would be considered “impaired.” There was unanimous agreement that Tier 2 represented reference conditions, and that Tier 3 clearly met the CWA interim goal for biological integrity. There was also unanimous ag reement that Tier 5 did not meet the CWA biological integrity goal. Approximately half the group thought the impairment threshold occurred between Tiers 4 and 5. The other half of the Florida experts propos ed the impairment line to be drawn between Tiers 3 and 4. Those panelists expressed a concern that man agement action may not be taken on sites until they were considered “impaired.” In other words, those panelists wanted the impairment line to include a buffer to allow more time for management action to prevent sites from becoming impaired. Taking theDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 47 recommendations of all 22 experts, we calculated the mid-point to define the line of impairment to be below Tier 4. From this definition for the line of impairmen t, BCG scores were related to SCI values to determine a corresponding numeric criterion for the suppor t of aquatic life use. Two statistical approaches were used: linear regression and change point analys is. Using linear regression, we found that an SCI value of 34 corresponded to a BCG score of 4 (Figure 16) . Based on this analysis, the impairment threshold could be set at an SCI value < 34. St ep function regression, also known as change point analysis, identified a significant difference (change or break point) corresponding to an SCI score of 36 ( Figure 17; Appendix I; Niu and Miller, 2007). Based on this analysis, the impairment threshold could be set at an SCI value < 36. Thus, the two different st atistical methods yielded very similar results. We averaged the SCI values derived from the two approache s to define the line of impairment to occur at SCI < 35. Using results from the BCG approach and the re commendations of 22 regional experts, SCI values from 0-34 are proposed as Category 3 (provisiona lly interpreted as “impaired”). SCI scores >= 35 represent Categories 2 and 1 (provisionally interp reted as “healthy” and “exceptional”; see below).

123456 

### Biological Condition Gradient score (mean) 0 20 40 60 80 100SCI

Figure 16. SCI values were highly correlated with the BCG scores assigned by the experts. Shown are the regression line (solid black) and its 90% confidence in tervals (dashed blue lines). Red dashed lines indicate the SCI threshold value of 34.4 based on a BCG score of 4 as the line below which biological impairment was defined.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 48 0 20 40 60 80 100 

## SCI 1

2

3

4

5

6Biological Condition Gradient score(median) Meets CWA Interim Goal for Biological Integrity Does Not Meet CWA Interim Goal for Biological Integrity

Figure 17. Change point model showing the relationship between the median BCG scores for each test sample and the corresponding SCI scores (change points = 35.8 and 9.1, alpha < 0.05).



## Defining biocriteria using reference site condition
Another U.S. EPA-recommended approach for setti ng the standard for biological condition involves analyzing reference sites. Reference sites may be defined as those with minimal human disturbance, approxim ating the “natural” condition of a region (U.S. EPA, 2006a, b; Hughes, 1995 Stoddard et al. 2006). From a set of reference sites, m easures of site condition, such as a multimetric index of biological condition, can be used to defi ne thresholds for acceptable and unacceptable biological condition. Lower percentiles, e.g., the 10 th or 25 th percentile, of the distribution of biological index values observed in reference sites are used by many states to define biological criteria. The selection of the specific percentile depends on the relative confidence associated with the initial selection of reference sites. Defining the limit of reference condition using a su bset of reference sites recognizes that natural stressors can affect the biology of a stream, introducing variability into t he reference site data distribution. In Florida, natural drought will cause dry or stagnant flow conditions, which may be associated with lower SCI values. Substrate diversity at some reference sites may be naturally limiting, which also may reduce SCI values. For Florida streams, our confidence was high that reference sites truly represented reference condition; therefore, we selected the 10 th (rather than the 25 th ) percentile.



### Reference site selection To develop the HDG, FDEP biologists selected site s from each of their regions that represented the broadest possible range of human influence, incl uding sites that represented the very best or minimally disturbed sites in their regions. In the early years of FDEP’s bioassessment program, every effort was made to identify the best examples of reference sites in the state. Over time, programDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 49 emphasis has shifted to other sampling needs, including status and trend monitoring, TMDL assessment, and springs studies (Figure 18) . Due to the extensive experience and knowledge of DEP regional biologists, most of the minimally disturbed stream sites have already been identified and sampled. In contrast, other states that start ed their biological monitoring programs later may have identified their reference sites from a probabilistic sampling design. T he criteria for selecting reference sites may be the same for both data sets, but the results may differ. Specifically, hand-picked reference sites in Florida may represent the very best sites available while othe r sites meeting the criteria for reference sites may have lower SCI values due to natural vari ability or minimal human disturbance. Reference sites were initially selected as site s with HDG = 0. Recall that HDG = 0 does not mean

no human disturbance, but minimal human distur bance. The criteria for HDG = 0 were:

● NH 3 < 0.1 mg/L

● Habitat Assessment Index > 65% of total points (scored from 0–100% of total points)

● Hydrological index < 6 (scored from 0–10), and

● LDI < 2.

Sites meeting these initial conditions were furt her scrutinized to eliminate sites potentially influenced by human activities not captured by the HDG. Additional criteria applied to reference sites included:

● Habitat Assessment Index > 75% of total points

● Site average conductivity < 600 umhos/cm

● No receiving sites for NPDES- permitted wastewater treatment fa cility discharges, based on a review of GIS coverage information from the Water Facilities Regulation (WAFR) database, and

● The number of individuals identified ranged from 100-175 (inclusive).

These criteria were established to select the very best reference sites from among sites with a HDG score of 0. The habitat assessment index crit erion was raised from 65% to 75% because sites scoring 75% or greater were equivalent to all or most of the habitat assessm ent parameters scoring as “Optimal” or “Suboptimal” (FDEP, 2004). The < 600 umhos/cm criterion for conductivity was based on results uncovered while evaluating a revision of the Class III freshwater specif ic conductance criterion (Chapter 62-302.530(61) F.A.C.) Th is study found that for a specif ic conductance of 20 umhos/cm approximately half of the taxa within a SCI samp le would be sensitive taxa and for 600 umhos/cm the proportion of sensitive taxa decreased to almost non e (FDEP, 2005). For the third criterion, sites could not be located below an active facility discharge based on review of GI S coverages and information from district biologists. A lower limit of 100 individuals was set as the minimum sample size for reference sites in order to be consistent with other state moni toring programs. This minimum of 100 individuals is consistent with a target of 100 or ganisms used by a majority of other state agencies (54% of 43 state programs surveyed; Carter and Resh, 2001). These criteria were established for selection of reference sites only; the application of the SCI is not limited to sites or samples conforming to these reference site criteria. Based on these criteria, 64 reference sites with a total of 205 site-visits were selected (Appendix J). For the 64 vetted reference sites, statistics were calc ulated from the distribution of reference site SCI values based on the median SCI value observed for each site. SCI values for reference sites ranged from 43.4–92.6 representing the natural variability associated with the SCI at sites with minimal human disturbance (Table 13) . The lower 10 th percentile SCI value for the reference site distribution was 48.8. Note that the 10 th percentile could have been derived in more than one way from the reference site scores. When we calculated these alternate st atistics they provided very similar SCI values. Examples include the 10 th percentile of SCI for all site -visits (44.9, N = 205); the 10 th percentile forDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 50 reference site mean values (SCI = 50.5, N = 64); the 10 th percentile for the most re cent site-visit (SCI = 50.2, N = 64).Number of sites1994199519961997199819992000200120022003200420052006 0 50 100 150 200 250 300 350

Status & Trends Basin Studies Reference sites 5th year sampling TMDL

Figure 18. Number of stream sites sa mpled for SCI by year and program.

Table 13. Statistics and percentile values for the SCI reference sites. Statistics and values for the distribution of SCI values at reference sites (N = 64).

Statistic Value

N 64 Minimum 43.4 10th percentile 48.8 25th percentile 56.7 Median 67.3 Average 66.3 Standard Deviation 12.6 75th percentile 75.2 90 th percentile 84.0 Maximum 92.6

Matching the approach of other states (EPA, 2006b) , we defined a buffer of SCI values below the 10 th percentile to represent the natural variability associated with field sampling that could be due to water level fluctuation, weather, or microhabitat differenc es. We used the MDD (calculated above in Chapter 2: Statistical Precision of Indexes ) to estimate the variability in SCI a ssociated with repeat sampling of the same stream site. Specifically, we subt racted half the MDD interval from the 10 th percentile to obtain aDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 51 lower limit of reference value of SCI = 35 (48.8 – 13.4 = 35.4). Thus, an SCI value of 35 represented the lowest SCI value we expect to obtain from a reference site.


### Distinguishing between Category 1 and 2 We used SCI values observed at reference sites to propose a threshold between Category 1 (“exceptional”) and Category 2 (“healthy”). The upper 90 th percentile of SCI values for the 64 reference sites was selected to represent exc eptional sites (SCI score of 84.0) . As for the impairment threshold definition above, we defined a buffer below the 90 th percentile using half the MDD interval (13.4) to yield an SCI value of 71 as the limit of Category 1 (84.0 – 13.4 = 70.6). The MDD interval provides a buffer around the point estimate of the lowest SCI val ue representing exceptional reference condition. As above, we note that the 90 th percentile could have been derived in more than one way from the reference sites. And, again as above, when we calc ulated these alternate statis tics they provided very similar SCI values. Examples include the 90 th percentile of SCI for all site-visits (86.7, N = 205); the 90 th percentile for reference site mean va lues (SCI = 83.8, N = 64); the 90 th percentile for the most recent sitevisit (SCI = 83.9, N = 64).



# Biocriteria for streams

The above analyses support the est ablishment of three categories of aquatic life use for Florida streams, described as Category 1 (“exceptional”, wi th SCI = 71–100), Category 2 (“healthy”, with SCI = 35–70), and Category 3 (“impaired”, with SCI = 0–34). Table 14 provides a narrative description for the numeric ranges of SCI

. The threshold values for the SCI were also used to define biocriteria for the BioRecon Index. Data were collected using both protocols at 112 sites. A linear regression equation was used to equate threshold values of SCI to corresponding values of the BioRecon Index. This analysis was used to define Category 1 (“exceptional”, with BioRecon = 6.5–10) , Category 2 (“healthy”, with BioRecon = 3.1–6.4), and Category 3 (“impaired”, with BioRecon = 0–3.0) stream condition. To have confidence in the final stream assess ment for regulatory decisions, two separate, temporally independent site-visits result ing in a Category 3 evaluation are required to list a stream site as failing to support aquatic life use. Table 14. Proposed aquatic life use categories, corresponding SCI values, and descriptions of biological conditions typically found for that category Narrative metric descriptions were not used to score metrics; rather, they describe values associated with a range of SCI values.
Aquatic life use category SCI Range Description

Category 1 71–100 Higher diversity of taxa than for Category 2, particularly for Ephemeroptera and Trichoptera; several more clinger and sens itive taxa than found in Category 2; high proportion for Tanytarsini; few individual s in the dominant taxon; very tolerant individuals make up a very small percentage of the assemblage

Category 2 35–70 Diverse assemblage with 30 different spec ies found on average; several different taxa each of Ephemeroptera, Trichopter a, and long-lived and, on average, 5 unique clinger and 6 sensitive taxa routinely found; small increase in dominance

by a single taxon relative to Category 1; very tolerant taxa represent a small percentage of individuals, but not iceably increased from Category 1

Category 3 0–34 Notable loss of taxonomic diversity; Ephemeroptera, Trichoptera, long-lived, clinger, and sensitive taxa uncommon or rare; half the number of filterers than expected; assemblage dominated by a toler ant taxon; very tolerant individuals represent a large proportion of the individuals collectedDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 52 

# CHAPTER 5: CONCLUSIONS

The correlation between biological metrics a nd the independently derived human disturbance gradient (HDG) provided strong evidence in support of the relationship between human influence and biological condition. The high correlation between the SCI and HDG was verified using an independent set of data. In addition, the pattern of biological resp onse to human disturbance was repeated across all 3 regions in Florida (panhandle, peninsula, and northea st), indicating that the multimetric index was independent of geographic context. Finally, the index was consistent across years, with a nearly identical relationship between the SCI and HDG for 10 years of data. To document every source of human disturbance is prohibitively expensive; consequently, the HDG was inevitably incomplete. We had no information on metals, pesticides, dredging, or other pollutants. Aerial pesticide application is a freque nt practice on agricultural lands and can be very damaging to stream invertebrates, as pesticides are concentrated in rain runoff (Hutchens et al., 1998). This lack of information may likely explain why low SCI values were observed for sites with no known source of disturbance. In sharp contrast, sites with known disturbance never had high values for the SCI. This result supports the idea that much of the lack of fit between disturbance and biological condition is largely due to measurement error associated with quantifying human disturbance, rather than the variability of biological measures (Bryce et al., 1999; Karr and Morishita Rossano, 2001; Fore, 2003). In short, multimetric indexes accurately synthesize the influence of human activities over time, while measures of human disturbance are at best approximations of the comp lex ways that human activities influence the environment. The SCI had good statistical precision and could det ect 3.7 categories of biological condition based on a single sample and 5 categories based on 2 samples. The BioRecon was not as precise but had adequate precision to detect 2.5 categories of bi ological condition based on a single sample and 3.5 categories for 2 samples. Strong correlation with an independent measure of dist urbance, good statistical precision, immunity to regional differences or wate rshed size, and minimal sensitivity to seasonal or yearly differences all contribute to the usefulness of these indexes for the biological assessment of Florida’s streams. We followed U.S. EPA guidance to translate index va lues into biocriteria to be included as part of the water quality standards for the st ate. Two approaches were used to define categories of biological condition. The primary approach involved conveni ng a panel of regional aquatic scientists to apply a nationally endorsed conceptual model of biological condition to Florida stream data, and was used to define the threshold of impairment (line between bi ological condition Categories of 2 and 3). The second approach used data from minimally disturbed areas (reference sites) to define expectations for reference condition and to define the threshold of exceptional sites (line between biological condition Categories of 1 and 2). Based on these results, three categories of biological condition are proposed to represent Category 1 (“exceptional”), Category 2 (“healthy)”,and Category 3 (“i mpaired”) biological condition for Florida stream site assessments. The results repo rted here provide the scientific basis for managing and protecting Florida’s stream resources.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 53 

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H . ———. 2006b. Best Practices for Identifying Referenc e Condition in Mid-Atlantic Streams. Washington, D. C.: Office of Environmental Information. EPA-260-F-06-002. Available: http://www.epa.gov/bioindicators/html/publications.html Vowell, J. L. 2001. Using stream bioassessment to monitor best management practice effectiveness. Forest Ecology and Management 143:237-244. White, W. A. 1958. Some geomorphic features of central peninsular Florida. Florida Bureau of Geology. Geological Bulletin 41. White, W. A. 1970. The geomorphology of the Florida peninsula. Florida Bureau of Geology. Geological Bulletin 51. Yoder, C. O., and J. E. DeShon. 2002. Using biologic al response signatures within a framework of multiple indicators to assess and diagnose causes and sources of impairments to aquatic assemblages in selected Ohio rivers and streams. In: Biological response signatures: Indicator patterns using aquatic communities, ed. T.P. Simon. Boca Raton, Florida: CRC Press LLC. Yoder, C. O., and E. T. Rankin. 1998. The role of biological indicators in a state water quality management process. Environmental Monitoring and Assessment 51:61-88.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 58 Zar, J. H. 1984. Biostatistical analysis, 2 nd ed. Englewood Cliffs, New Jersey: Prentice-Hall, Inc.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 59 

# APPENDICES

Appendix A. HDG calculation
This appendix details the calculations and interim steps involved in deriving an HDG value for each site. The HDG combined 4 measures of human disturbance (NH 3 , habitat index, hydrologic index, and LDI) by first converting them to unitless scores (representing low, medium and high disturbance) and then summing the scores. When multiple site visits occurred at a single sampling location, the individual component measures were averaged for each site and then converted to unitless scores. For a site with multiple visits, NH 3 , the habitat index, and, in a few instance s, the hydrologic index, could change. LDI could not change because it was calculated only once for each site. When component values were missing, they were assumed to be in good condition and scored as 0. 

## Coding for NH 3 Many of the reported NH 3 values had qualifiers associated with them. In some cases the qualifiers were omitted and the results were used as reported and in others the value was simply set to 0 to simplify coding for the HDG (Table A

- 1). Another more common approach is to record half of the detection limit rather than 0, but for calculating the HDG this interim recoding did not matter because either value would be scored as a 0 for the HDG.

Table A-1. Laboratory codes associated with water chemistry values, their description, and the

action used to convert them to simple numbers. All the codes are listed but J and Q were not found for the NH 3 values.

CODE Description ACTION

A A-Value reported is the mean of two or more determinations omitted A qualifier and used result as reported

I I- The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit set result to 0

J J-Estimated Value omitted J qualifier and used result as reported

Q Q-Sample held beyond normal holding time omitted Q qualifier and used result as reported

U or BDL U-Material analyzed for but not detected; value reported is the minimum detection limit set result to 0

T T- below detection limit; estimate set result to 0Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 60 

## Derivation of habitat index values
Data for this study were collected from 1991–2001. During that time the habitat assessment protocol was modified and additional measurements were added. During these different time periods the maximum possible total score changed. To make com parisons of the habitat index across years, we divided habitat index values by the total maximum score that was possible for that protocol. In this way the habitat index was transformed into a per centage measure that ranged from 0–100%. 

## Derivation of hydrologic index values
At the April, 2002, Biocriteria Meeting, a traini ng session on the hydrologic index scoring process was conducted. The purpose for assigning the hydr ologic modification scores was to develop one of several components of the HDG. FDEP biologists we re instructed to use their knowledge of human activities within a watershed and upstream of selected sampling sites in order to assign a score from 1 to 10, following the specific criteria presented (s ee Table 2, main document). The key issues for consideration in the scoring process were:

• The stream’s hydrograph response to a rain event.

• The amount of ditching or impoundments wi thin the watershed that would alter normal water deliveries to the stream.

• Topographic alterations in the watershed that would adversely affect water deliveries to the stream.

• Water withdrawals (consumptive use) in the area that may reduce water to the stream.

• The relative amount of impervious surfac es in the watershed that would “spike” the hydrograph. Since the participants had simultaneously visited ce rtain streams as part of previous Biocriteria Meeting training and quality assurance activities, appro priate hydrologic index sc ores for these example streams were discussed, to promote consis tency in the application of the process. 

## Scoring component measures of the HDG
In order to calculate the HDG, the 4 component measures were first transformed into unitless scores (see Table 5, main document, for scoring rules). The first round of data was used to derive the break points for scoring the measures. The graphs used to define the break points are shown below for each of the 4 component measures. To define the scor ing rules, natural break points in the data were used. When no break points were obvious, the ra nge of the data was divided into approximately equal parts.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 610.0030.0040.0050.0060.0070.0080.0090.0100.0200.0300.0400.0500.0600.0700.0800.0900.1000.2000.3000.4000.5000.6000.7000.8000.9001.0002.0003.0004.0005.0006.0007.0008.0009.00010.00020.00030.000

NH3 -2 0 2 4 6 8 10 12 14 16 18Number of EPT taxa

Figure A-1. Scoring rules for NH 3 for inclusion in the HDG. NH 3 plotted against a logarithmic axis.

10 20 30 40 50 60 70 80 90 100 110 Habitat index (%) -2 0 2 4 6 8 10 12 14 16 18Number of EPT taxa

Figure A-2. Scoring rules for the habitat index for inclusion in the HDG.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 62 0 2 4 6 8 10 12 Hydrologic index -2 0 2 4 6 8 10 12 14 16 18Number of EPT taxa

Figure A-3. Scoring rules for hydrologic index for inclusion in the HDG.

0123456789 LDI index (buffer) -5 0 5 10 15 20 25 30 35Number of EPT taxa

Figure A-4. Scoring rules for the LDI index for inclusion in the HDG.Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200763Appendix B. Long-lived taxaTaxonomic order, family, genus, or species name of taxa used tocalculate long-lived taxa richness. Taxonomic synonyms presentin the currentFlorida database are also shown. Long-lived (semivoltine) taxarequire more than one year to complete their life cycles.OrderFamilyGenusSpecies (Long-lived)SynonymsDecapodaAll families, genera, and speciesBasommatophora LymnaeidaeFossariaAll speciesMesogastropoda AmpulariidaePomaceaAll speciesVeneroida CorbiculiidaeCorbiculaCorbicula flumineaCorbicula manilensisUnionoidaUnionidaeAll genera and speciesOdonata AeshnidaeBasiaeschna Basiaeschna janataBoyeriaAll speciesCordulegastridaeAll speciesGomphidaeGomphus All species GomphurusHaegenius Hagenius brevistylusProgomphusAll speciesLibellulidaeMacromiaAll speciesSomatochloraAll speciesTetragoneuriaAll speciesEpithecaPetaluridaeTachopteryx Tachopteryx thoreyiDevelopment and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200764OrderFamilyGenusSpecies (Long-lived)SynonymsPlecoptera PteronarcidaePteronarcys Pteronarcys dorsataLeuctridaeLeuctraAll speciesPeltoperlidaeTallaperla TallaperlacorneliaPerlidaeAcroneuriaAll speciesAgnetina Agnetina annulipesEccoptura Eccoptura xanthenesAcroneuria xanthenesNeoperlaAll speciesParagnetinaAll speciesBanksianaBanksiellaPerlodidaeClioperla Clioperla clioIsoperla clioPerlinellaPerlinella drymoAtoperla ephyrePerlinella ephyre/zwickiIsoperla Isoperla orataMegalopteraCorydalidaeAll genera and speciesTrichoptera BrachycentridaeBrachycentrus Brachycentrus americanus Oligoplectrum amercanumMicrasemaAll speciesCalamoceratidaeHeteroplectron Heteroplectron americanumHydropsychidaeDiplectrona Diplectrona modestaMacrostemum Macrostemum carolina Macronema carolinaMacronemum carolinaLepidostomidaeLepidostomaAll speciesAlepomyia, Alepomyiodes,Arcadopsyche, Atomyia,Jenortha, Mormomyia,Neuropsyche, Nosopus,Notiopsyche, Olemira,Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200765OrderFamilyGenusSpecies (Long-lived)SynonymsOligopsyche, Phanopsyche,Pristosilo, AthripsodesLeptoceridaeCeracleaAll speciesLimnephilidaeIronoquia Ironoquia punctatissismaPycnopsycheAll speciesMolannidaeMolannaAll speciesPhryganeidaeBanksiola Banksiola concatenata Neuronia concatenataPtilostomis Ptilostomis postica Neuronia posticaAgrypnia Agrypnia vestitaPsychomyiidaeLype Lype diversaRhyacophilidaeRhyacophilaAll speciesUenoidaeNeophylaxAll speciesDevelopment and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200766Appendix C. Sensitive taxaTaxonomic order, family, genus or species name; taxonomic synonyms in the current Florida database; whether the taxon was included in theFlorida Index (2 = very sensitive; 1 = sensitive); and species name of Florida Index taxa are listed for taxa identified as sensitive to humandisturbance.OrderFamilyGenusSpecies (sensitive)SynonymsFlorida indexAcariformes LebertiidaeLebertiaAll speciesIsopoda AsellidaeCaecidotea CaecidoteaAsellus 1Caecidoteaspp.Amphipoda CrangonyctidaeCrangonyx CrangonyxEphemeroptera BaetidaeAcerpenna Acerpenna pygmaeaBaetis pygmaeusEphemerellidaeAll genera and speciesHeptageniidaeAll genera and species1Stenacron interpunctatumStenonema mexicanumintegrum2Stenonema exiguumStenonema smithaeLeptophlebiidaeAll genera and speciesLeptohyphidaeAll genera and speciesOdonata LibellulidaeMacromiaAll species2Macromiaspp.PlecopteraAll families, genera andspecies2 AllPlecopteraTrichoptera HydropsychidaeHydropsycheAll species2Hydropsychespp.LeptoceridaeTriaenodesAll speciesPhilopotamidaeChimarra Chimarra2Chimarraspp.Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200767OrderFamilyGenusSpecies (sensitive)SynonymsFlorida indexPsychomyiidaeLype Lype diversaColeoptera ElmidaeAncyronyx Ancyronyx variegatusGonielmis Gonielmis dietrichiMacronychus Macronychus glabratusDiptera ChironomidaeMicrotendipesAll speciesParametriocnemusAll speciesPolypedilum Polypedilum avicepsRheocricotopusAll species 2Rheocricotopus robackiStempellinellaAll speciesTanytarsus Tanytarsus sp. d eplerTanytarsus Tanytarsus sp. m eplerTribelos Tribelos jucundumEmpididaeHemerodromia HemerodromiaSimuliidaeAll genera and species2Simuliumspp.Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200768Appendix D. Very tolerant taxaTaxonomic order, family, genus or species name; taxonomic synonyms in the current Florida database; whether the taxon was included in theFlorida Index (2 = very sensitive; 1 = sensitive); and species name of Florida Index taxa are listed for taxa identified as very tolerant to humandisturbance.OrderFamilyGenusSpecies (very tolerant)SynonymsFlorida IndexHoplonemertea TetrastemmatidaeProstoma Prostoma rubrumHaplotaxida NaididaeBratislavia BratislaviaunidentataDeroAll speciesNaisNais communiscomplexNais communisNaiselinguisNaispardalisNaisvariabilisPristina Pristina synclitesTubificidaeHaber Haber speciosusLimnodrilusAll speciesCamptodriluscalifornicusLimnodrilusaurantiacusLumbriculida LumbriculidaeLumbriculusAll speciesRhynchobdellida GlossiphoniidaeAll genera and speciesBasommatophora AncylidaeHebetancylus Hebetancylus excentricusLaevapexAll speciesPhysidaePhysellaAll speciesPlanorbidaeAll genera and speciesDevelopment and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200769OrderFamilyGenusSpecies (very tolerant)SynonymsFlorida IndexMesogastropoda HydrobiidaePyrgophorus Pyrgophorus platyrachisPyrogophorusplatyrachisThiaridaeMelanoidesAll speciesOdonata LibellulidaePachydiplax Pachydiplax longipennisCoenagrionidaeArgia Argia sedula2Argia sedulaEnallagma Enallagma cardenium Enallagma coecumIschnuraAll species AnomalagrionHeteroptera MesoveliidaeAll genera and speciesLepidoptera PyralidaeAll genera and speciesColeoptera HaliplidaePeltodytesAll speciesDiptera ChironomidaeLarsiaAll species 1Larsia lurida1Larsia decolorataChironomus AllspeciesTendipesChaetolabisCladopelma CladopelmaCricotopusAll species2Cricotopus bicinctusCryptochironomusAll speciesDicrotendipes Dicrotendipes modestusDicrotendipesneomodestusGlyptotendipesAll speciesGoeldichironomusAll speciesKiefferulusKiefferulusDevelopment and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200770OrderFamilyGenusSpecies (very tolerant)SynonymsFlorida IndexParachironomusAll speciesPolypedilum Polypedilum beckaeAsheum beckaePedionomus beckaePolypedilumillinoiensegrp.Polypedilumillnoense1Polypedilum illinoiensegrp.Polypedilum tritum Chironomus tritumTanypusAll species PelopiaTanytarsus Tanytarsus sp. f eplerStratiomyidaeOdontomyia OdontomyiaEulaliaTipulidaeTipula TipulaNippotipulaPlayttipulaYamatotipulaDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 71 

# Appendix E. Regional calibration of SCI metric scores

After metrics were selected based on thei r correlation with the human disturbance gradient (HDG), they were transformed to unitless scores to compensate for differences in their ranges of values. For example, percentage filterer might range from 0 to 60% while long-lived taxa range only from 0 to 7. The 5 th and 95 th percentiles for each metric were used to determine the metric values that would be defined as 0 and 10 (Table E-1) . To control for differences due to natural variability associated with different bioregions, t he percentiles were calculated within each of the 3 bioregions. The next step tested for regional differences in metrics scores by regressing metric scores for each bioregion against the HDG and comparing the slopes of their lines. If the regression lines for each bioregion failed to matc h closely, the endpoints for the metrics that defined values of 0 and 10 were adjusted away from the 5 th and 95 th percentiles and tested again until the regression lines for the different bioregions yielded a similar relationship with HDG (Figure E-1; Table E-2) . In some cases, the 5 th percentile values were very close and they were simply averaged across bioregions for simplicity. After determining which metric values define t he scores of 0 and 10, the remaining metric values between those endpoints must also be scored ( Table E-3; FDEP, 2007). As an example, for taxa richness in the northeast bioregion the interval between the 2 endpoints is 42 – 16 which equals 26. If the lowest endpoint is 16, we would first subtract 16 from the total taxa richness observed at a site, divide by the interval length of 26, and multiply the result by 10 to obtain scores that range from 0 to 10. Taxa richness values less than 16 would be scored as 0 and values greater than 42 would be scored as a 10.

Table E-1. SCI metric name, 5 th and 95 th percentile values by bioregion for site-visits with

75–175 individuals. Underlined values indicate that the metric values used to define a score of 0 or 10 were slightly diffe rent from the percentile value.

SCI Metric NE 5 th %tile PH 5 th %tile PS 5 th %tile NE 95 th %tile PH 95 th %tile PS 95 th %tile

Total taxa 17.0 16.0 16.0 42.0 46.0 41.0

Ephemeroptera taxa 0.0 0.0 0.0 4.0 6.0 5.0
Trichoptera taxa 0.0 0.0 0.0 7.0 7.0 7.0

% Filterer 1.4 1.3 0.9 52.0 45.1 40.0
Long-lived taxa 0.0 0.0 0.0 3.0 5.0 4.0
Clinger taxa 0.0 0.0 0.0 10.0 14.0 8.0

% Dominance 10.9 9.6 11.6 47.2 42.6 54.5
% Tanytarsini 0.0 0.0 0.0 27.9 22.8 26.8

Sensitive taxa 0.0 0.0 0.0 11.0 16.0 9.0
% Very tolerant 0.0 0.0 0.8 78.2 36.3 58.5Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 72 Table E-2 (Table 8 from main SCI document). SCI metric name, adjusted metric values

used to scores metrics (endpoints of the range represent scores of 0 and 10), and comment describing how the values differed from the 5 th and 95 th percentiles. Underlined values correspond to the underlined values in Table E-1.

SCI Metric Northeast Panhandle Peninsula Comment

Total taxa 16 –42 16– 49 16–41 Averaged the 5 th %tile and used same value in all bioregions Ephemeroptera taxa 0– 3.5 0–6 0–5 Lowered expectations for 95 th %tile for NE Trichoptera taxa 0– 6.5 0–7 0–7 Lowered expectations for 95 th %tile for NE
% Filterer 1–42 1–45 1–40 Lowered expectations for 95 th %tile for NE Long-lived taxa 0–3 0–5 0–4 Clinger taxa 0– 9 0– 15.5 0–8 Lowered expectations for 95 th %tile for NE; raised for PH % Dominance 54

– 10 43–10 54– 10 Averaged lower 5 th %tile; % Tanytarsini 0–26 0–26 0–26 Raised expectations for 95 th %tile for NE
Sensitive taxa 0–11 0– 19 0–9 Raised expectation for 95 th %tile for PH % Very tolerant 78–0 36–0 59–0

Table E-3. SCI metric scoring formulae for converting metric values to a metric score ranging from 0 to 10. In each equation, ‘X’ stands for the raw metric value; “ln” stands for the natural log.

SCI metric Northeast Panhandle Peninsula

Total taxa 10 * (X–16)/26 10 * (X–16)/33 10 * (X–16)/25 Ephem. taxa 10 * X /3.5 10 * X /6 10 * X /5 Trichoptera taxa 10 * X /6.5 10 * X /7 10 * X /7
% Filterer 10 * (X–1)/41 10 * (X–1)/44 10 * (X–1)/39 Long-lived taxa 10 * X /3 10 * X /5 10 * X /4 Clinger taxa 10 * X /9 10 * X /15.5 10 * X /8
% Dominance 10 – ( 10 * [ ( X–10)/44 ] ) 10 – ( 10 * [ ( X–10)/33 ] ) 10 – ( 10 * [ ( X–10)/44 ] ) % Tanytarsini 10 * [ ln( X + 1) /3.3] 10 * [ ln( X + 1) /3.3] 10 * [ ln( X + 1) /3.3]
Sensitive taxa 10 * X /11 10 * X /19 10 * X /9 % Very tolerant 10 – (10 * [ ln( X + 1)/4.4 ] ) 10 – (10 * [ ln( X + 1)/3.6 ] ) 10 – (10 * [ ln( X + 1)/4.1 ] )Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 73 Human disturbance gradientMetric score for % filterer

-2 0 2 4 6 8 10 0 2 4 6 8 10 Northeast Panhandle Peninsula

Human disturbance gradientMetric score for % filterer

-2 0 2 4 6 8 10 0 2 4 6 8 10

Figure E-1. Metric scores for percent filterer by bi oregion. The upper panel shows metrics scored according the 5 th and 95 th percentiles and the lower panel shows scores after adjustment. The regression line for the NE bioregion was lower than the other 2 regions initially and plotted higher and closer to the other 2 lines after adjustment.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 74 

## References

Florida Department of Environmental Protecti on (FDEP) 2007. Department of Environmental Protection Standard Operating Procedures for Laboratory Activities. Table LT7200-1 of LT 7200 Stream Condition Index (SCI) Determination in DEP-SOP-002/01. LT 7000 Determination of Biological Indices. Available:

1U http://www.dep.state.fl.us/labs/sop/index.htm

H .Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200775Appendix F. ANOVA output and regression results for the SCI and BioReconTable F-1. General linear model output for the SCI versus HDG by region (northeast, panhandle, or peninsula)Neither the region nor the interaction of the HDG and region was statistically significant. Test failed to detect a differencein SCI values due toregion.Univariate Tests of Significance for AvgOfSCI6_100 (1NGM_4)SSdfMSFpIntercept 403623.3 1 403623.3 1832.253 0.000000Region361.62180.80.8210.441846HDG2 62750.7 1 62750.7 284.858 0.000000Region*HDG21142.92571.52.5940.077660Error 37449.0 170 220.3Table F-2. General linear model output for the SCI versus HDG by year class (1992–93, 1994–95, 1996–97, 1998–99, and 2000–01)The interaction term was not significantly different for year class and the HDG, suggesting the lines were parallel (equal slopes). The year classalone, however, was significant, with valuesfor 1994–95 being slightly higher for the SCI.Univariate Tests of Significance for SCI6_100 (1NGM_4)SSdfMSFpIntercept1627060116270605835.6570.000000Year class294847372.6440.032766HDG21344971134497482.3900.000000Year class*HDG279141980.7090.586005Error171749616279Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200776Table F-3. ANOVA estimates of SCI variability for repeat visits to the same site within a yearError estimate used to calculate confidence interval is underlined.ANOVA for SCI (SCI6_100) (Same_day_SCI)EffectdfMSdfMSFp{1}STORET Random 60 1211.636 39.41542 188.7279 6.420014 0.000000{2}YearRandom6269.43035.76223170.24021.5826480.1807271*2 Random 53 134.481 82.0000066.59522.019385 0.002054Table F-4. ANOVA estimates of SCI variability for laboratory subsamplingError estimate used is underlined.ANOVA Results: SCI subsampling (Metrics)EffectdfMSdfMSFp{1}Sitename Random 58 1393.986 11832.8631242.41795 0.00Table F-5. ANOVA estimates of BioRecon variability for repeat visits to the same site within a yearError estimate used to calculate confidence interval is underlined.ANOVA for BioRecon (BR3_10) (New metrics multiple_2)EffectdfMSdfMSFp{1}STORET Random 126 15.91691 117.3771 1.696009 9.384924 0.000000{2}year Random 8 6.02260 156.00001.4588404.128352 0.000171Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 77 

# Appendix G. Optimal sample size for calculating SCI D. Whiting, J. Espy, and L. Wolfe (FDEP)

Taxa richness increases with the number of individuals identified from a sample. This relationship is well documented for many studies (Larsen and Herlihy, 1998), and is further supported by recent analyses conducted for FDEP which are described below. Because the SCI includes several metrics that quantify taxa richne ss, the concern here is that SCI values may be artificially increased or decreased depending on the number of individuals identified from a sample. Therefore, laboratory methods were modifi ed in recent years (2004–2006) to ensure that the number of individuals identified is more consistent across samples. The SCI data used to develop and test the SCI and BioRecon were collected between 1991–2001. The laboratory protocols used during that time mandated a target number of 100 individuals. In practice, however, this target was often exceeded due to the nature of the laboratory subsampling methods (many samples had several hundred individuals). During this early time period, FDEP did subsample material collected from the field; however, laboratory protocols did not allow multiple divisions of the material to achieve the 100-individual target. For the data set used to test and develop SCI, only si te-visits with 75–175 individuals were used in order to more closely approximate the target nu mber of individuals. Thus, metric expectations and scoring rules for SCI were based on samples with between 75 and 175 individuals (mean 122, median 119). Restricting the index developm ent data set to samples with only 100 to 110 individuals would have reduced the number of sa mples available for the recalibration effort dramatically, thereby reducing confidence in the resulting biomonitoring tool. The target number of organisms for the 2004 laboratory protocols for the SCI was 100 organisms (acceptable range 100-110). Such a na rrow acceptable range was made possible by modifying the 20-dip net sweep composite sample preparation Standard Operating Procedure. This modification resulted in the samples consistently being on target. Random site sampling in 2004 from the ambient monitoring program yielded an adequate number of samples ( n = 201) to compare the effect of the more accurate laboratory subsampling methods on metric values. We compared the percent iles of the metric values used to define the scoring rules for the SCI and the percentiles of metr ic values from this more recent data set. For the 1992-2001 sampling, 420 samples with between 75 and 175 individuals were used. For the 2004 sampling, 196 samples were used. Approximat ely even numbers of samples were available for the 2004 data in the peninsula and panhandle r egions; not enough sites were sampled in the northeast to calculate percentiles. This comparison was not ideal because the sampling designs were very different. The 2004 sites were selected randomly while the earlier sites used in this study were selected for a variety of specific reasons (e.g., references sites, 5 th years inspections, special studies, etc.). Thus, the distribution of percentile values may be shifted due to the laboratory methods or the types of sites sampled. If both data sets had been created from random sampling, the comparison would more clearly isolate the effect of the change in laboratory methods. As expected, total taxa richness showed t he largest differences with lower values observed in 2004 (Table G-1) . Other taxa richness metrics were also lower at the 95 th percentile. Ephemeroptera and long-lived taxa were lower in peninsula region, Trichoptera had a similar distribution, and clinger taxa were lower in the panhandle region. The 95 th percentiles for sensitive taxa were lower in both regions. Developing scientific protocols for use in a regulatory framework is inevitably an iterative process. Our understanding of the sources of variability and the influence of precision on assessment methods has evolved rapidly in recent y ears. If we were to start over with no data onDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 78 hand and develop a biological monitoring protocol, the number of individuals to identify from each sample might be one of the first elements that we would define. The iterative nature of this process derives from the fact that knowledge gained regarding one aspect of the analysis methods often necessitates that methods re lated to another aspect of the analysis be reconsidered. A large portion of the variability in SCI values was tied to laboratory methods, and subsequently, improvements have been made in t hat area. This change should be understood as

a calibration of the monitoring protocol. The fundamental relationships between biological metrics and human influence are unaffected by this issue. A recent analysis by the FDEP Central Laborato ry biologists has found that averaging the SCI scores from two 150-organism subsamples provides a more reliable assessment than a single 100-organism subsample. T he objective of this analysis was to evaluate how laboratory processing and/or the number of individuals ident ified affects SCI score variability. For this analysis, all the organisms found in one-third (33%) of the material brought to the laboratory for each of three samples were identified. Using those data, 250 random draws were conducted for each sample for each of six different target num bers of individuals: 100, 125, 150, 175, 200, and 300 (Figures G-1 to G-3)

Table G-1. Percentiles of metric values for the development data set and random sampling in 2004 Shown are the 5 th and 95 th percentile values for SCI metrics from sites sampled during 1992– 2001 (SCI development data set) and 2004 (ambient sampling).

Panhandle Peninsula

1992- 2001 2004 1992- 2001 2004 1992- 2001 2004 1992- 2001 2004

Percentile 5th 5th 95th 95th 5th 5th 95th 95th

Total taxa 16.0 13.0 46. 0 37.0 16.0 13. 0 41.0 35.0 Ephemeroptera taxa 0.0 0.0 6.0 5.0 0.0 0.0 5.0 3.0

Trichoptera taxa 0.0 0.0 7.0 6.0 0.0 0.0 7.0 7.0
% Filterer 1.3 0.9 45.1 45.7 0.9 0.0 40.0 32.0 Long-lived taxa 0.0 0.0 5. 0 5.0 0.0 0.0 4.0 2.0

Clinger taxa 0.0 0.0 14.0 9.0 0.0 0.0 8.0 7.0
% Dominance 9.6 11.9 42. 6 55.3 11.6 13. 2 54.5 68.0 %Tanytarsini 0.0 0.0 22.8 31.8 0.0 0.0 26.8 20.0
Sensitive taxa 0.0 0.0 16.0 11.0 0.0 0.0 9.0 7.0 % Very tolerant 0.0 0.0 36.3 48.1 0.8 1.0 58.5 79.8Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 79 

## Hatchet Creek

100 125 150 160 175 200 300



## Target number of individuals 30 40 50 60 70 80 90 100 110SCI

Avg of 2 150's Median 25%-75% Non-Outlier Range Outliers

Figure G-1 . Box plots showing the change in SCI score with target number of individuals counted for 250 random draws of one-third of a sample from Hatchet Cr eek. Shown also is the box plot for the average SCI score from 2 150 subsamples.



## Mule Creek

100 125 150 160 175 200 300



## Target number of individuals 40 50 60 70 80 90 100 110SCI

Avg of 2 150's

Figure G-2 . Box plots showing the change in SCI score with target number of individuals counted for 250 random draws of one-third of a sample from Mule Cree k. Shown also is the box plot for the average SCI score from 2 150 subsamples.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 80 

## Crooked Creek

100 125 150 160 175 200 300



## Target number of individuals 40 50 60 70 80 90 100 110SCI

Avg of 2 150's

Figure G-3 . Box plots showing the change in SCI score with target number of individuals counted for 250 random draws of one-third of a sample from Crooked Cree k. Shown also is the box plot for the average SCI score from 2 150 subsamples.

FDEP Central Lab biologists believe the increase in precision associated with larger and multiple subsamples is worth the cost, as it make s the calculated result less variable and provides greater confidence in the tool. Prior to the sample preparation SOP being modified to more accurately achieve the target number of organi sms, the sample processing time at the FDEP Central Laboratory averaged 18 hours per sample. The revision to the SOP resulted in the processing time being reduced by 56% (approximately 8 hours). While the increase from 100 organisms to 300 organisms (150x2) results in a significant increase in laboratory workload (average processing time based upon preliminary data is about 14 hours), the increase is balanced by the earlier changes to the sample pr eparation SOP that result in the analyst more accurately achieving the target num ber of organisms. The result is the identification of three times as many organisms for approximately a 75% increase in laboratory effort. A survey of 43 state agencies’ programs found that approximately 54% of those programs targeted 100 organisms (Carter and Resh , 2001). The next most commonly used target number of organisms was 300 (26% ). All other target number of organisms were used by < 7% of the programs. Additionally, U.S. EPA has used a target number of organisms of 300 for the National Wadeable Stream s survey (EPA, 2006). The FDEP decision to use 150X2 subsamples was made based upon four key points:

● First, the 150x2 subsampling will result in 300 organisms being identified, which was the second most common subsampli ng target number in the survey, and which is consistent with U.S. EPA minimum subsampling target used for the National Wadeable Streams survey

● Second, the use of 150x2 subsamples rather than one 300 target subsample maintains scoring within the range used to calibrate the SCI (75-175)Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 81 ● Third, targeting 150 organisms in each subsample, rather than 125 organisms (approximately the mean number of organisms in the calibration dataset samples used to score the SCI metrics), results in a slightly higher score at good quality sites, while poor quality sites scores do not appear to increase similarly. This effect results in slightly better discriminati on between good, moderate, and poor quality sites

● Fourth, the use of 150x2 subsamples reduc es the variability in the resulting SCI score by using the average of two 150-organi sm-based. This reduction in variability improves consistency with regard to the assessment of category thresholds.


## References

Carter, J. L. and V. H. Resh. 2001. After site selection and before data analysis: sampling, sorting, and laboratory procedures used in stream benthic macroinvertebrate monitoring programs by USA state agencies. Journal of the North American Benthological Society 20:658-682. Larsen, D. P. and A. T. Herlihy. 1998. The dile mma of sampling streams for macroinvertebrate richness. Journal of the North Amer ican Benthological Society 17:359-366. U. S. Environmental Protection Agency (EPA). 2006. Wadeable Streams Assessment: A Collaborative Survey of the Nation's Streams. EPA 841-B-06 -002. Washington, D. C.: Office of Water. Available: http://www.epa.gov/owow/streamsurvey/

.Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 82 

# Appendix H. FDEP Biological condition gradient workshop summary Russel Frydenborg and Denise Miller (FDEP)


## Background
Florida Department of Environmental Pr otection (FDEP) staff evaluated stream macroinvertebrate data from the stream condition index (SCI) recalibration dataset with the goal of refining delineations of biological/ecologica l condition based on the SCI 2004 scores. Several approaches were taken for this evaluation, am ong them a Biological Condition Gradient (BCG) Workshop which was conducted by FDEP staff on Oc tober 24, 2006. This document provides a summary of FDEP’s BCG workshop. A more detailed description of the workshop and materials is provided in Frydenborg and Miller, 2007. In preparing for this workshop, FDEP staff used the framework of prior such workshops conducted by Susan Davies of the Maine Depa rtment of Environmental Protection, Susan Jackson of the U.S. Environmental Protecti on Agency (Davies and Jackson, 2006), and Jeroen Gerritsen of Tetra Tech, Inc. Each of those individuals also contributed thoughtful commentary during the planning of this workshop. For the FDEP BCG workshop, a group of experts in aquatic biology and stream macroinvertebrate identification were requested to review taxonomic data from a subset of samples from the SCI 2004 recalibration data set and to provide scores for those samples using the BCG framework (scoring based on descriptions of BCG attributes per experts’ review of the taxonomic data). Each expert would thus provide their assessment of the biological condition of each of 30 samples selected by FDEP to span the range of SCI scores from the SCI 2004 recalibration data. Experts did not know the SCI scores for the samples. FDEP evaluated the experts’ BCG scores together with SCI scores for the samples as one line of evidence in determining what delineations or thresholds of SC I scores should be used in regulatory decisionmaking (e.g., what distribution of scores signifies that a site is meeting designated use or not meeting designated use). FDEP invited participants for this workshop who have expertise in Florida stream macroinvertebrate identification, and who hail from local and state regulatory agencies, universities and the private sector.


## Methods
Prior to the workshop, experts were pr ovided with background literature on the biocondition gradient concept (Davies and Jacks on, 2006; Stoddard et al., 2006) and descriptions from FDEP of the intended process (Frydenborg an d Miller, 2007: Attachment A). Experts were informed that they would be asked to review SCI sample data that would be provided to them at the workshop and to assign each sample a score of 1 to 6, signifying their assessment of the biological condition represented by the sample (1 best to 6 worst based on the 6 tiers of the conceptual BCG summarized from Davies and Jackson, 2006: Figure 2):

• Score of 1: Native or natural condition

• Score of 2: Minimal loss of spec ies; some density changes may occur

• Score of 3: Some replacement of sensit ive-rare species; functions fully maintained

• Score of 4: Some sensitive species maintained but notable replacement by moretolerant taxa; altered distributi ons; functions largely maintainedDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 83 • Score of 5: Tolerant species show increasing dominance; sensitive species are rare; functions altered

• Score of 6: Severe alteration of structure and function.

At the workshop, Russel Frydenborg, FDEP’s workshop moderator, gave a presentation describing the BCG process and attributes, and di stributed materials from the presentation for reference by the experts (Frydenborg and Miller, 2007: Attachment B). During the workshop, experts we re given the opportunity to discuss their rationale for scoring of a site, and, if they desired, to cha nge their score for a site based on their assessment from the discussions (Delphi method). Scoring s heets were provided to each expert for recording both initial and final sample scores. Experts were requested to indicate what relevance they ascribed to their BCG scores. More specifically , they were asked to record the minimum BCG score(s) (1 being best, 6 worst) that they be lieved met the interim goals of the Clean Water Act (meets designated use), and what maximum score(s) they believed did not meet the interim goals of the Clean Water Act (impaired site; does not meet designated use). In other words, sites below a selected tier score would be considered “impaired.” 

## Sample Selection
Sample data were drawn from the SCI recali bration data and were limited to those where the total number of individual organisms counted in the sample was between 100-175, inclusive. Five (5) reference site (minimally disturbed c ondition site) samples from each of three (3) Florida Bioregions (Panhandle, Peninsula, Nort heast) were selected to allow the experts an assessment of reference expectations separately for each region because prior SCI analysis indicated differences in expectations for some metrics between the bioregions (Table H-1) . The 15 reference site samples were selected from sites already identified as reference based on criteria defined independently of the biological condition (see Chapter 4: Reference site selection ). The 5 reference samples from each bioregion were selected to span the range of SCI scores for all of the reference site samples from that bioregion, including minimum and maximum SCI 2004 scores, and samples with scores closest to the 25 th percentile, median, and 75 th percentile values from each bioregion reference range. SCI scores were used for sample selection, but were not provided to the experts . Additionally, experts were not provided with information about the location or description of t he selected sample sites, other than the bioregion from which the samples were collected. Additionally, 10 samples were selected for each bioregion, and identified to bioregion, for analysis of macroinvertebrate data and scoring by the experts. Sites were selected to span a range of conditions using existing information on SCI scores and Human Disturbance Gradient (HDG), and, as needed, LDI from the SCI recalib ration data set. The specific 10-sample selection methodology for each bioregion was as follows (Table H-2) :

• 4 samples selected based on those hav ing SCI scores closest to the 10 th , 25 th

, median and 75 th percentile SCI scores for the non-reference sites for the bioregion;

• 2 samples randomly selected from non-reference sites for the bioregion having SCI scores in the range of 35-50 (sorted on SCI score), to ensure scrutiny of some samples in this score range;

• 3 samples randomly drawn from HDG ranges of 1-2, 3-5, and/or 6-9, based on the assessment of the HDG scores already repr esented from the prior 6 sample selection (sorted on HDG scores);

• For the Northeast bioregion samples only, there were insufficient sites with the target HDG ranges (i.e., no HDG scores of 5 through 9, and only one HDG 4 site). Therefore, 2 of the intended HDG-based sa mples were randomly drawn based on theDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 84 range of LDI scores for the samples, afte r an assessment of the LDI scores already represented from the prior 7-sample selection. Specifically, there were 6 samples in the 1 to < 2 LDI range, and 1 sample in the LDI 6 to < 7 range. Therefore, 2 samples were randomly selected from the LDI 2 through 9 range (sorted on LDI scores), excepting sites with LDIs >= 6 and < 7; and,

• 1 sample randomly drawn from the entir e non-reference dataset for the bioregion (sorted on HDG score).

Table H-1. BCG workshop reference sites Site identification information, bioregion locati on, date of sampling, SCI value, and LDI value.

BCGSampleID STORET Station Nickname Bioregion Sample Date SCI LDI

NERef01 20030341 CECFLD7 No rtheast 3/5/1996 70.4 1.90

NERef02 21010018 FALLNGREF Northeast 7/27/1999 39 1.37

NERef03 19010099 PIGEONREF Northeast 2/1/2000 58 1.27

NERef04 21010056 ROBCKCR131 Northeast 3/13/2000 79.2 1.35

NERef05 20030456 THOMASREFB No rtheast 3/14/1996 92.4 1.25

PanhRef01 33040014 BIGHORSREF Panhandle 8/23/1994 67 1.23

PanhRef02 32010024 LIMEREF P anhandle 7/14/1998 74.5 1.33

PanhRef03 33010065 RSTAREARUN Panhandle 2/14/1996 54.7 1.19

PanhRef04 22030010 STMARKSREF Panhandle 9/18/2001 40.5 1.29

PanhRef05 31010051 SWTREF Panhandle 7/8/1992 97.8 1.31

PenRef01 28020237 CYPCKUNK P eninsula 7/21/1999 55.7 1.18

PenRef02 20030414 GLDHEADREF Peninsula 1/12/2000 100 1.00

PenRef03 24010002 MANAREF Peni nsula 11/21/2000 80.2 1.31

PenRef04 20020012 OKLRIVREF Peninsula 2/15/2000 68.1 1.01

PenRef05 20030253 PUTNA44 P eninsula 3/16/1998 24 1.15Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 85 Table H-2. BCG workshop samples

BCG SampleID STORET Station Nickname Bioregion Sample Date SCI HDG LDI Selection Rationale

NE01 19020027 ALIGTRREF Northeast 4/11/ 2001 47.1 1 1.4 SCI [35-50] random

NE02 19010049 BLDWIN REF Northeast 2/14/2000 32.9 2 3.22 Random

NE03 19020053 CALAHANTST Northeast 9/24/2001 24.4 3 1.41 SCI 10th Percentile (22.96)

NE04 19020053 CALAHANTST Northeast 7/15/1996 40.1 3 1.41 SCI [35-50] random

NE05 20030549 CECFLD6 Northeast 3/6/ 1996 73.6 1 2 LDI [2-9] random

NE06 20030332 JAXHEITTST Northeast 5/5/1992 28.9 4 6.11 HDG [3-5] random

NE07 21030059 LKBUTTST Northeast 9/15/ 1992 9.1 3 5.26 LDI [2-9] random

NE08 19010073 MCCLNYTST Northeast 3/22/1999 68 0 1.55 SCI 75th Percentile (68.27)

NE09 20030573 MILOG209 Northeas t 1/26/1998 30.6 1 1.61 SCI 25th Percentile (30.39)

NE10 19020059 YULEEREF Northeast 10/28/1996 47 0 1.28 SCI 50th Percentile (45.09)

Panh01 22020078 ABHOPTST Panhandle 6/ 4/1996 20.7 3 1 HDG [3-5] random

Panh02 33040073 CANEYCBELL Panhandle 8/22/2000 30 3 1.71 HDG [3-5] random

Panh03 31010125 CHATTATST Panhandle 10/13/1998 62.5 0 1.62 SCI 50th Percentile (62.40)

Panh04 31020071 CHTMDLCRKD Panhandle 7/23/1996 70.5 1 1.72 SCI 75th Percentile (70.50)

Panh05 31020072 CHTMDLOTTR Panhandl e 7/23/1996 43.5 2 1.42 Random

Panh06 33010112 COFECSTLR Panhandle 2/16/1999 47.7 2 1.05 SCI [35-50] random

Panh07 31020042 DOZREF Panhandle 9/1/1992 30.2 6 2.83 SCI 25th Percentile (30.10)

Panh08 31020043 DOZTST Panhandle 9/1/1992 35.8 6 2.75 SCI [35-50] random

Panh09 22030064 FLAMINREF Panh andle 3/8/1993 4.6 7 8.08 SC I 10th Percentile (4.92)

Panh10 22050003 TAYLO4 Panhandle 4/10/2000 0.6 3 2.28 HDG [3-5] random

Pen01 20030585 BUNCNTRL Peninsula 9/8/ 1998 15.5 6 5.29 HDG [6-9] random

Pen02 25020550 DESOT34 Peninsula 9/3/1997 37.5 4 2.91 SCI [35-50] randomDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 86 BCG SampleID STORET Station Nickname Bioregion Sample Date SCI HDG LDI Selection Rationale

Pen03 27010580 GROOVRARPT Peninsula 8/22/2000 24.2 1 1.79 SCI 25th Percentile (24.55)

Pen04 24040117 GULFTST Peninsula 10/8/1998 14.8 7 7.85 SCI 10th Percentile (14.45)

Pen05 24030044 HILSTP5REF Peninsula 8/18/1993 55 1 2.12 HDG [1-2] random

Pen06 20010384 LONGBRANCH Peninsula 7/13/1999 38.2 3 2.53 SCI [35-50] random

Pen07 28010224 NWFRLOXREF Peninsula 9/20/19 95 40.8 4 3.87 SCI 50th Percentile (40.81)

Pen08 25020014 OAKREF Peninsula 8/22/1995 58.3 1 2.1 SCI 75th Percentile (58.70)

Pen09 20020145 SWEETCK Peninsul a 8/10/1998 21.1 2 1.3 Random

Pen10 20020420 WGCITREF Peninsula 12/1/1992 0 7 8.32 HDG [6-9] random



## Data Content

Data provided for each sample, whether for reference samples or samples for BCG scoring, consisted only of the biological data for each sample and an identification of the sample to the Bioregion of its collection. Other data rega rding the sample and site (e.g., more specific location, collection date, information on physic al/chemical characterist ics, human disturbance metrics such as LDI, hydrologic alteration) were not provided to the experts, but were available for analysis with the scoring data from the workshop.

Biological data that were provided for each sample were retrieved from the FDEP Statewide Biological database (SBIO). Those data included the collapsed taxa list with the number of individuals of each taxon counted in that sample and a suite of metrics. Details regarding the SBIO taxa collapsing procedure are provided in the SBIO database manual (Wolfe and Lurding, 1999: p. 84, and Appendix 2). Fryde nborg and Miller, 2007 (Attachment C) provides a copy of each sample sheet of the taxa list and metrics that was provided to experts, a copy of a list of synonyms that was provided to assist in their review of the taxa list, and a copy of the sample scoring sheet.



## Results

Each expert provided a BCG score for each site sample (Table H-3) and an assessment of the interpretation that should be applied to each BCG score (e.g., meets designated use, does not meet designated use; Table H-4 ). In several cases, experts reported a “gray” area in the relevance they ascribed to their BCG scores. Se veral experts identified BCG scores they judged as clearly meeting designated use, those they judged as clearly not meeting designated use, and left a gap between those scores signifying their uncertainty about where such a line could beDevelopment and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 87 drawn. Responses were unanimous that a BCG score of 3 or lower (1-3) meets the CWA interim goal for biological integrity and that BCG score s of 5 and 6 do not meet the CWA biological integrity goal. Approximately half the panel thought the impairment threshold occurred between BCG scores of 4 and 5. The other half of the panel wished the impairment line to be drawn between BCG scores of 3 and 4, with many of those panelists expressing the concern that management action may not be taken on sites until they were considered “impaired.”



## References

Davies, S.P. and S.K. Jackson. 2006. The biologic al condition gradient: a descriptive model for interpreting change in aquatic systems. Ec ological Applications 16(4):1251-1266. Frydenborg, R. and D. Miller. 2007. Biological Co ndition Gradient Workshop, October 24, 2006. Florida Department of Environmental Protection. Stoddard, J.L., D.P. Larsen, C.P. Hawkins, R.K. Johnson, and R.H. Norris. 2006. Setting expectations for the ecological condition of streams: the concept of reference condition. Ecological Applications 16(4):1267-1276. Wolfe, L.E. and K.M. Lurding. 1999. Statewide Biological Database. Florida Department of Environmental Protection.

1U ftp://ftp.dep.state.fl.us/pub/l abs/biology/biodb/dbmanual.pdf

H .Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200788Table H-3. BCG workshop scores summaryExpert CodeE01E02E03E04E05E06E07E08E09E10E11E12E13E14E15E16E17E18E19E20E21E22MedianBCG ScoreSCIScoreBCGSampleIDFinal BCG ScoreNE013434333334334343333234347.1NE02 5 5 5 5 5 5 5 5 5 5 4 4 5 5 5 5 5 4 4 4 5 5 5 32.9NE034555555555555554555535524.4NE04 3 4 4 3 4 4 3 4 3 3 3 3 3 3 3 3 3 3 3 3 4 4 3 40.1NE052122212222222122212112273.6NE06 4 5 4 5 3 5 5 5 3 5 5 5 4 5 5 4 4 4 4 5 4 4 5 28.9NE07665666666666666666565669.1NE08 3 3 3 2 3 4 5 3 3 3 4 3 2 3 2 3 3 3 3 3 3 3 3 68NE094454534455444454444444430.6NE10 3 3 4 2 3 4 4 3 3 3 2 3 3 3 4 3 3 3 3 3 3 3 3 47Panh015445555444354454454344420.7Panh02 5 4 4 4 5 5 5 5 4 5 4 4 5 5 5 4 5 5 5 4 4 5 5 30Panh032222212222222122122231262.5Panh04 2 2 2 2 2 2 2 2 2 2 2 3 2 1 2 1 1 2 2 2 2 2 2 70.5Panh054432443433434333334333343.5Panh06 3 2 2 2 2 2 2 3 2 2 2 2 3 2 2 2 2 2 2 2 3 2 2 47.7Development and Testing of Biomonitoring Tools for Macroinvertebrates in Florida StreamsMarch 200789Expert CodeE01E02E03E04E05E06E07E08E09E10E11E12E13E14E15E16E17E18E19E20E21E22MedianBCG ScoreSCIScorePanh075555555555455555555445530.2Panh08 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 5 4 5 5 5 35.8Panh09666656566666666666566664.6Panh10 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 0.6Pen015565565655555656655566515.5Pen02 3 3 3 4 3 3 3 4 4 4 3 3 4 4 3 4 4 3 4 3 3 3 3 37.5Pen035444544455445545455454424.2Pen04 5 5 5 5 5 5 5 5 5 5 4 5 5 6 5 6 5 5 5 5 5 6 5 14.8Pen053222222322222222333222255Pen06 4 4 4 3 5 4 4 4 5 4 4 4 4 5 4 4 4 4 4 4 4 4 4 38.2Pen073322333333333343333333340.8Pen08 2 2 1 1 2 2 2 1 1 2 1 2 1 2 2 2 2 2 2 2 2 2 2 58.3Pen095555545655555555555565521.1Pen10 6 6 6 6 6 5 6 6 6 6 6 5 6 6 6 6 6 6 6 6 6 6 6 0Development and Testing of Biomonitoring Tools fo r Macroinvertebrates in Florida Streams March 2007



### 90 Table H-4: Experts’ categorization of BCG workshop scores

Expert Code: Not Impaired/ Meets Goal Gray Area Does Not Meet Goal/Impaired Comments

E01 3 4
E02 3 4 5

E03 3 Not impaired 3- 4, closer to 4, but not below 4 5

E04 4 5
E05 4 5
E06 3 4
E07 3 4
E08 3 4
E09 3 4
E10 3 4
E11 3 4
E12 4 5
E13 3 4

E14 4 5 Impairment line 4-5, but concerned that the category 5 sites will not be improved and category 4 sites will slip into category 5 and then may be clumped with the other 5's as "irretrievable"
E15 3 4

E16 3 line between 4 and 5 5

E17 3 Impairment Line 4-5, 4 probably meets 5

E18 4 5
E19 4 5
E20 4 5
E21 4 5
E22 4 5

### 91 

# Appendix I. Change Point Analysis of Stream Condition Index and Biological Condition Gradient Data Xufeng Niu (Florida State Univer sity) and Denise Miller (FDEP)


## Background
For the purpose of scrutinizing Stream Condi tion Index (SCI) macroinvertebrate data with the goal of refining delineations of biological/ecological condition, Florida Department of Environmental Protection (FDEP) staff conducted a biological condition gradient (BCG) workshop in October 2006 (Frydenborg and Miller, 2007). Participants in the BCG workshop (the panel) consisted of twenty-two (22) experienced aquatic biologists in Florida who agreed to assist FDEP as part of this delineation effort. During the BCG workshop, the panel was provi ded with species lists and metrics for 15 reference streams and 30 test samples, equally divided into Florida’s three bioregions (Panhandle, Northeast, and Peninsula). The panel wa s not given the SCI scores associated with any of the sites, or any physical, chemical, or habitat descriptions of the sites (other than the Bioregion location). Decisions were based on biological community composition data collected via the 20-dip net sweep method associated with SCI sampling. The scores (1 through 6) were based on the six tiers of the conceptual biological condi tion gradient (BCG) from Davies and Jackson, 2006, listed below:

• Score of 1: Native or natural condition

• Score of 2: Minimal loss of specie s; some density changes may occur

• Score of 3: Some replacement of sensitiv e-rare species; functions fully maintained

• Score of 4: Some sensitive species ma intained but notable replacement by moretolerant taxa; altered distributi ons; functions largely maintained

• Score of 5: Tolerant species show in creasing dominance; sensitive species are rare; functions altered

• Score of 6: Severe alteration of structure and function

The analyses conducted in this report were based on the results from the BCG workshop (BCG scores for 30 samples by each of 22 panelis ts) and the SCI scores for those same samples to address FDEP’s goal of refining delineations of biological/ecological condition for SCI scores.


## The Detection Procedure
Niu et al. (2000) introduced an iterative pr ocedure for detecting and modeling level-shift change points. The procedure is similar to that suggested by Chang (1982) and further developed by Chang et al. (1988) for detecting outliers and level shifts in time series analysis. Statistical details of this procedure can also be found in Pankratz (1991, Chapter 8). For simplicity, let us consider a response variable Y , after an appropriate transformation. Suppose that observations { 

### ( ,) i i 

### X Y

, 

### 1, 2, ... , i n

= } are available where n is the sample size
and X is an independent variable. Moreover, we assume that the observations are arranged in the following manner:

### 92 • The values { 

### , i 

### X 

### 1, 2, ... , i n

= } are distinct. If several 

### ' i 

### Y s are corresponding to a single X value, the median of the 

### ' i 

### Y s is taken to be the response value for the X
value.

• { 

### ( ,) i i 

### X Y

, 

### 1, 2, ... , i n

= } are sorted according to the values of X from least to greatest.

For each integer 

### 1 l > , define the step variable 

### () 0 i 

### S l = for 

### i l < and 

### () 1 i 

### S l = for 

### i l

≥

.
Step 1 . Fit the linear regression model:

0 1



### () () () () i i i 

### Y l lSl l β β ε =+ +
, 

### 1, 2, ... , i n

= , (1)

where for a fixed l , the 

### ( )' i 

### l s ε are assumed to be independent and identically distributed

normal random variables with mean zero and variance 2 

### ( ) l σ

.

Step 2 . Calculate the values { 1 1 

### ( ) ( ) / ( ( )) L l l se l β β = ) )

, 

### 2, 3, ... , ( 1) l n

= − } where

1 

### ( ( )) se l β ) is the estimated standard error of 1 

### ( ) l β )

.

Step 3 . Let 1 

### ( ) max{ (2), (3), ... , ( 1)} Ll L L Ln = − and compare 1 

### ( ) L l with the critical
value C =3.0 (or C =3.5). The critical value C =3.0 (or C =3.5) corresponds roughly to 

### 0.10 

### α 

### = (or 

### 0.05 α = ), or the 10% (or the 5%) significance level, based on the simulation results of Chang et al. (1988). If 1 

### ( ) L l is significant, we conclude that the response Y has a change point at

1 l 

### X

with a level-shift 1 

### ( ) l 

### β )

.

Step 4 . Let * 11 1 

### () () ii i 

### Y Y lSl β = − . Repeat Steps 1-3 on the new response variable

* i 

### Y for detecting a possible second change poin t. Continue the process until no further change point can be identified.

Step 5 . Suppose that k change points are detected in the response variable Y and the corresponding X values are

1 2 

### { , , ... , }

k ll l 

### X X X . Fit the model

0 11 2 2 

### ( ) ( ) ... ( ) i ii k i k i 

### Y Sl Sl Sl β ββ βε = + + ++ +
, 

### 1, 2, ... , i n

= . (2)

Then the estimated coefficients 1 2 

### { , , ... , } k 

### β β β ) ) ) will be the k estimated level-shift
values.

### 93 

## Analysis of SCI and BCG Data

Change Point Analysis

Table I-1 contains a list of the SCI values for the 30 samples from the BCG workshop along with their median and mean BCG scores ba sed on the individual scores given by the experts. Change-point analysis of the SCI values was conducted in this report using the median BCG scores as the dependent variable. Median BCG scores rather than mean BCG scores were used to avoid potential outlier problems. Two change points were detected in the SCI values. The first change point was at SCI=35.8, which has the statistic 

### L(l 1 

### ) = 9.79 and is significant at the 5% level (95% confidence). The second change point occurred at SCI=9.1 which has the statistic 

### L(l 1 

### ) = 3.87 and is also significant at the 5% level (95% confidence). Th e R-square of the regression is 0.86, indicating that the step-function regression model fits the median BCG scores very well.

Table I-1. SCI and BCG scores for the 30 Samples

STORET Station Sample Date SCI Score BCG Median Score BCG Mean Score

20030549 3/6/1996 73.6 2 1.7

31020071 7/23/1996 70.5 2 1.9
19010073 3/22/1999 68 3 3

31010125 10/13/1998 62.5 2 1.9
25020014 8/22/1995 58.3 2 1.7

24030044 8/18/1993 55 2 2.2
33010112 2/16/1999 47.7 2 2.2

19020027 4/11/2001 47.1 3 3.2
19020059 10/28/1996 47 3 3.1

31020072 7/23/1996 43.5 3 3.3
28010224 9/20/1995 40.8 3 3

19020053 9/24/2001 40.1 3 3.3
20010384 7/13/1999 38.2 4 4.1

25020550 9/3/1997 37.5 3 3.4

31020043 9/1/1992 35.8 5 5
19010049 2/14/2000 32.9 5 4.8
20030573 1/26/1998 30.6 4 4.2

31020042 9/1/1992 30.2 5 4.9
33040073 8/22/2000 30 5 4.6

20030332 5/5/1992 28.9 4.5 4.4
19020053 9/24/2001 24.4 5 4.8

27010580 8/22/2000 24.2 4 4.5
20020145 8/10/1998 21.1 5 5

22020078 6/4/1996 20.7 4 4.3

### 94 STORET Station Sample Date SCI Score BCG Median Score BCG Mean Score

20030585 9/8/1998 15.5 5 5.4

24040117 10/8/1998 14.8 5 5.1

21030059 9/15/1992 9.1 6 5.9

22030064 3/8/1993 4.6 6 5.9
22050003 4/10/2000 0.6 6 6

20020420 12/1/1992 0 6 5.9

Change Points: 1). SCI=35.8 with the test statistic of 9.79 and confidence level > 95%; 2). SCI=9. 1 with the test statistic of 3.87 and confidence level > 95%. Highlighted numbers are the SCI-BC G Scores at the change points.



### Confidence Intervals for the Change Points
In this section, we constructed confidence intervals for the detected change points based

on a modified bootstrapping method. “Regula r” bootstrapping is a statistical method for estimating the sampling distribution of an estimator by resampling with replacement from the original sample (Efron, 1979; Efron and Tibshirani, 1993). This me thod is widely used in parameter estimation, regression, time series, and other statistical problems. In regression analysis, there are two basic approaches to perform bootstrapping. The first approach is bootstrapping cases. Assume that we want to fit a regression model with response
variable Y and predictors { 1 

### , ... , ... p 

### x x }, and we have a sample of n cases

1 

### { ( , , ... , ... ), 1, ... , } i i i pi 

### Z Yx x i n 

### = = . In bootstrapping, we select n cases randomly from the original observations with replacement. For each bootstrapping sample, we fit the regression model and save the coefficients. Confidence in tervals for the regression coefficients can be constructed based on M (a given large number) bootstrapping samples. The second approach in bootstrapping regression analysis is resampling the residuals. In this way a regression model is fitted to the original data first. The fitted values and residuals are calculated based on the fitted model. The residuals are randomly resampled and added back to the fitted values. After generating a new set of bootstrapping data , the regression model is refitted. Efron and Tibshirani (1993, Chapter 9, p. 113) pointed out that bootstrapping cases is less sensitive to model assumptions such as normality and constant variance than bootstrapping residuals. In this study, the bootstrapping ca ses method was used to construct confidence intervals for change points. There are several approaches to constructing bootstrap confidence intervals for change points. The bootstrap percentile interval approach was used in this study. This approach uses the empirical quantiles of 

### ˆ θ to form a confidence interval * * 

### ˆ ˆ [, ] Lower Upper 

### θ θ for the change point θ

,
where ** * (1) (2) ( ) 

### ˆˆ ˆ { , , ... , } M 

### θθ θ are the ordered bootstrap estimates of the change point; * 

### ˆ
Lower 

### θ

and * 

### ˆ Upper 

### θ are the α /2 percentile and the (1

− α /2) percentile of the bootstrap estimates of the change point, respectively. For this analysis, we had 30 pairs of SC I and Median BCG Scores. We used a modified bootstrapping method to construct confidence interv als for the change points. Specifically, we

### 95 first randomly drew 90% of the 30 pairs (or dr opped 10%) from the original data and conducted change-point analysis. This procedure is also called the drop-d Jackknife method in statistics (Efron and Tibshirani, 1993, Page 149). Three thousand (3,000) samples were drawn from the original data, and the results are presented in T able I-2. For the first change point at SCI=35.8, the 90% and 95% confidence intervals based on the percentiles of the 3,000 estimated change points were identical.

Table I-2. Confidence Intervals (CI) for the change points of SCI scores based on M= 3,000 drop-10% samples.

First Change Points Second Change Points

SCI Based on Original Data 35.8 9.1

SCI Based on Average of drop-10% Estimates 35.8 8.8

SCI Based on Median of drop-10% Estimates 35.8 9.1

Standard Error of the Change Point 1.6 2.7

SCI Percentile Interval, 95% CI [32.9, 38.2] [4.6, 13.4]

SCI Percentile Interval, 90% CI [32.9, 38.2] [4.6, 12.0]



## Summary
This change-point analysis was conducted for the purpose of addressing FDEP’s goal of refining delineations of biological/ecological c ondition for Stream Condition Index (SCI) scores. The BCG (biological condition gradient) scores that were used to evaluate changes in SCI scores were provided by a panel of 22 experienced aquatic biologists in Florida. The results of this change-point analysis indicated a first change point at SCI=35.8, with the 95% confidence interval [32.9, 38.2]. In other words, SCI scores higher (b etter) than 35.8 were determined to be different from lower scores based on the biological condition assigned to taxonomic data for the samples by experienced aquatic biologists. Additional det ails of the BCG workshop and the change-point analysis are provided in Frydenborg and Mille r, 2007 and Niu and Miller, 2007, respectively.


## References
Chang, I. 1982. “Outliers in Time Series," Unpublished Ph.D. Dissertation, Department of Statistics, University of Wisconsin, Madison. Chang, I., G.C. Tiao, and C. Chen. 1988. “Estimation of Time Series Parameters in the Presence of Outliers," Technometrics , 30: 193-204. Davies, S.P. and S.K. Jackson. 2006. “The Biologi cal Condition Gradient: A Descriptive Model for Interpreting Change in Aquatic Systems.” Ec ological Applications. 16(4): 1251-1266. Ecological Society of America. Efron, B. 1979. “Bootstr ap Methods: Another Look at the Jackknife.” Annals of Statistics 7:1-26. Efron, B. and R. J. Tibshirani. 1993. An Introduction to the Bootstrap . New York: Chapman and Hall.

### 96 Frydenborg, R. and D. Miller. 2007. “Biological Co ndition Gradient Workshop, October 24, 2006”. Florida Department of Environmental Protection. Niu, X., P. Lin, and D. Meeter. 2000. “Detecting Change Points in the Species Composition and Water Quality Data of WCA2A”. Department of Statistics, Florida State University, Tallahassee. Technical Report Submitted to the Florida Department of Environmental Protection. Niu, X. and D. Miller. 2007. “Change Point Analys is and Model Comparison of Stream Condition Index and Biological Condition Gradient Data”. Technical Report Submitted to the Florida Department of Environmental Protection. Pankratz, A. 1991. Forecasting with Dynamic Regression Models. John Wiley & Sons Inc. New York. Schwartz, G. 1978. Estimating the Dimension of a Model. The Annals of Statistics , 6: 461-464.97Appendix J. Reference sitesTable J-1. Reference site locations.LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec20030245 Ates Creek @ CR 315 Northeast BLACK CREEKNo point sources or stormwater inputs upstream29 54 38 81 53 520030437BLACK CR., NORTHPRONG, 1/4 ABOVE BIGBRANCHNortheast BLACK CREEKOne large stormwater constructin permit upstream, possiblyconnected through wetlands issued in 6/2005.30 6 48.13 81 54 23.3420030419 Black Creek, South Prong Northeast BLACK CREEKCamp blanding is upstream of this site29 56 3.15 81 55 9.6320030723N. FORK BLACK CRKABOVE CARTERSPENCER RD.Northeast BLACK CREEKDupont Highland Mine facility discharges, but this site is abovethis. 30 49.281 58 43.920030247South Fork Black Creek @SR 16 Northeast BLACK CREEK No point sourcesor stormwater inputs upstream 29 58 45 81 51 1020030570Bradley Crk @ 739B-Peter'scrk basinNortheast BRADLEY CREEKSubdivision (Silver Creek) stormwater permits (2003 &2005),discharge may flow towards this site.30 2 48.8 81 47 5.520030487Bull Creek @ NE Crossingof CR 215 Northeast BULL CREEK No point sources orstormwater inputs upstream 30 2 3.2 81 54 6.819010042Calkins Ck, off HampRegister Rd.Northeast CALKINS CREEK No point sources orstormwater inputs upstream 30 19 50.95 82 14 30.4721010043Deep Creek upstream ofbridge @Old River Rd Northeast DEEP CREEKNo point sources or stormwater inputs upstream30 22 35.7 82 38 16.121010018Falling Creek @ CR 131,above falls Suwannee R.Northeast FALLING CREEKKOA Domestic WWTF ~3000 m upstream of site, not NPDESpermitted, design capacity = 0.0150. Not NPDES permitted, andsmall design capacity likely means that this is a percolation pond.District Biologist is not aware of a KOA discharge in this area.30 15 39.47 82 40 5.1919010064John Rowe Br, test site forNE FL State Hosp FYI Northeast JOHN ROWE BRANCHNo point sources or stormwater inputs upstream; The hospitaldoesn't show up as permitted site on the GIS coverage 30 13 47.4 82 8 16.498LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec20030571Peter's Crk aboveRosemary hill Rd- Basin Northeast PETERS CREEKClay Co. Dept. of Env. Services has 2 stormwater permits right atthe site and 2 stormwateroutfall sites30 0 25.9 81 44 55.419010099Pigeon Creek at US 1 St.Marys-Nassau RiverNortheast PIGEON CREEK No point sources or stormwater inputs upstream30 45 40.73 81 58 3.0721010056PCS Phosphate FYI5,control site Northeast ROBINSON CREEK No point sources or stormwater inputs upstream 30 19 14 82 39 44.121010032Rocky Ck @ WoodpeckerRdNortheast ROCKY CREEK No point sources or stormwater inputs upstream30 32 40.27 82 44 1.9120030265Sal Taylor Creek belowRowell Creek Northeast SAL TAYLOR CREEKOne large stormwater constructin permit above these sites issuedin 12/2004. 30 11 59 81 54 5021030115Santa Fe Rv- Control ABMPNortheast SANTA FE RIVER A few stormwaterpermits pretty far upstream29 50 46.9 82 12 13.820030456Thomas Ck, Forestry BMPStudy Ref Site B Northeast THOMAS CREEK No point sources or stormwater inputs upstream29 54 57.6 81 51 44.919010072 McClenny WWTP upstream Northeast TURKEY CREEKA few stormwater permits around, look to be small and may notactually discharge to this stream30 16 6.4 82 7 34.920030341Yellow Water Creek aboveSal Taylor Creek Northeast YELLOW WATER CREEKOne large stormwater constructin permit above these sites issuedin 12/2004. 30 12 3.2 81 55 10.3520030342Yellow Water Creek belowSal Taylor CreekNortheast YELLOW WATER CREEKOne large stormwater constructin permit above these sites issuedin 12/2004.30 11 44.45 81 55 5.822020070BIG CYPRESS BRANCHTIMBER ENERGY FYICNTRL SITEPanhandleBIG CYPRESS BRANCH No point sources or stormwater inputs upstream30 19 0 84 48 033040014BIG HORSE CREEK S.R.2OKA.CO.PanhandleBIG HORSE CREEK No point sources orstormwater inputs upstream 30 55 19.3 86 35 42.621030064Cedar Head Run, aboveIchetucknee RiverPanhandleCEDAR HEAD RUNIchetucknee Family Campground WWTF upstream of site, notNPDES permitted (?), designcapacity=0.007. Subsequentadditional information indicates no discharge. Stream begins inState Park. 29 58 50.2 82 45 30.9231010050CROOKED CREEKHWY.270 GADSDEN CO.PanhandleCROOKED CREEKNo point sources or stormwaterinputs upstream 30 34 59.79 84 52 51.4999LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec32030023ECONFINA CREEK ATSCOTT RD PanhandleECONFINA CREEK Nopoint sources or stormwater inputs upstream30 33 19.3 85 26 622050077Econfina River ForestryBMP Study Ref Site BPanhandleECONFINA RIVER No point sources orstormwater inputs upstream 30 9 52.8 83 50 14.633040064Jacks Branch, Forestry BMPStudy Ref Site B PanhandleJACK'S BRANCH No point sources or stormwater inputs upstream 30 52 10.3 86 29 21.732010024Limestone Ck @ BeckridgeRdPanhandleLIMESTONE CREEK No point sources orstormwater inputs upstream 30 59 21.58 86 5 9.8132020063LITTLE CROOKED CREEKS.R.79 BAY CO. PanhandleLTL CROOKED CREEK No point sources or stormwater inputs upstream30 24 48.4 85 52 6.522030062MCBRIDE SLOUGHHWY.267 WAKULLA CO.PanhandleMCBRIDE SLOUGH No point sourcesor stormwater inputs upstream 30 14 10 84 16 2033010054MCDAVID CREEK AT SR99 PanhandleMCDAVID CREEKNo point sources or stormwater inputs upstream 30 44 21.6 87 26 54.822020049MULE CREEK S.R.12LIBERTY CO.PanhandleMULE CREEK No point sources or stormwater inputs upstream30 30 43.55 84 49 44.122020062Oklawaha Ck, SR 267, S ofQuincy PanhandleOKLAWAHA CREEKSand mine operations (permitted IWF's). Subsequent additionalinformation indicates no discharge to sampled site. 30 27 2.18 84 38 36.8931020073Pitts Mill Creek above confwith Cypress CkPanhandlePITTS MILL CREEK No point sources or stormwater inputs upstream30 16 43.9 85 14 23.622020010QUINCY CR above SR12,ref for Quincy STP FYIPanhandleQUINCY CREEKEngelhard IWF upstream and numerous other small IWF's just Sthat may/may not flow towards this site. Subsequent additionalinformation indicates no discharge to site except maybe duringhurricanes. 30 35 34.6 84 33 50.322030010St. Marks R approx 4 mi Nof Hwy 98 (@ "rapids") PanhandleST. MARKS RIVER No point sources or stormwater inputs upstream 30 14 24.8 84 8 43.431010051SWEETWATER CREEKHWY.270 LIBERTY CO.PanhandleSWEETWATER CREEK No point sources orstormwater inputs upstream 30 31 26.68 84 58 25.15100LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec31020040TEN MILE CREEK S.R.73CALHOUN CO. PanhandleTEN MILE CREEKOne small WWTP and one Industrial Wastewater permittedfacility upstream; Stormwater construction at sampling point. WWfacilities at least 1.5 to 2 km upstream. Additional subsequentinformation from District Biologist is that site is appropriate forreference. 30 30 1.27 85 12 1.9133010065Rest Area Run Creek belowI 10PanhandleUNNAMED CREEKSurrounded by stormwater construction, potential inputs fromPerdido landfill upstream. Subsequent additional information fromDistrict Biologist is that this sitewas reference at the time relevantto the SCI sampling for the calibration dataset.30 34 5.4 87 24 10.622040038Unnamed Creek aboveMonticello WWTP PanhandleUNNAMED CREEKA few stormwater permits around, look to be small and may notactually discharge to thisstream 30 30 59.5 83 51 25.332020066Wright Ck, ref for NomaSTP FYIPanhandleWRIGHTS CREEK Site is upstream of Noma WWTP 30 58 47.7 85 35 54.420010455BLACKWATER CREEK ATHWY 44A Peninsula BLACKWATER CREEK Nopoint sources or stormwaterinputs upstream 28 52 38.96 81 29 19.9128020239 Cypress brch above SR 78 Peninsula CYPRESS BRANCH Nopoint sources or stormwater inputs upstream26 48 49 81 20 728020237Cypress Creek 2 mi aboveSR78Peninsula CYPRESS CREEKSmall stormwater constructionpermit for Wayne Homes; permitstarted in 9/200426 45 5 81 37 1426010972DAVENPORT CR AT SR545 BRIDGE Peninsula DAVENPORT CREEK Gas pipeline relocationstormwater permit startedin 2/2005 28 16 15.2 81 35 28.0626010593FISHEATING CREEKABOVE US 27 Peninsula FISHEATING CREEKLykes Campground WWTF nearby, not NPDES permitted (?);Depending on flow direction could impact site. Subsequentadditional information indicates that site is upstream fromcampground.26 56 12 81 19 3420030414GOLD HEAD BR. IN STATEPARK Peninsula GOLD HEAD BRANCH No point sourcesor stormwater inputsupstream 29 49 56.03 81 56 46.6424030142Hillsborough R upstrm ofSouthside Br, ZephyrhilPeninsula HILLSBOROUGH RIVERZephyrhills WWTF upstream ofsite, but can't determine whereoutfall is. Lots of monitoring wells around. Subsequent additionalinformation indicates probably a percolation pond. No dischargeto the site - the site was just visited. 28 11 8.4 82 10 30.9101LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec24030048 Hillsborough River at SR39 Peninsula HILLSBOROUGH RIVERZephyrhills WWTF upstream ofsite, but can't determine whereoutfall is. Lots of monitoring wells around. Subsequent additionalinformation indicates no discharge to the site - the site was justvisited. 28 11 38.7 82 10 2.825020111Horse Creek @ SR 72BridgePeninsula HORSE CREEK No point sources or stormwater inputs upstream27 11 59.91 81 59 15.2820010454JUNIPER CREEK ATHIGHWAY 19 Peninsula JUNIPER CREEK No point sourcesor stormwater inputsupstream 29 12 49.41 81 39 17.520020004Little Orange Creek @ Hwy21Peninsula LITTLE ORANGE CREEKGa Pacific-Hawthorne Plywood STW discharges upstream, butmay flow into Little Orange Lake. Subsequent additionalinformation indicates no discharge to the stream. 29 32 28.42 81 57 16.6726011019LIVINGSTON CREEK ATRUCKS DAIRY RD. Peninsula LIVINGSTON CREEK No point sources or stormwaterinputs upstream27 42 29.61 81 26 49.0228010223N FK LOXAHATCHEERIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER No point sources orstormwater inputs upstream27 0 4.76 80 6 58.4824010002Manatee R 20 m belowSR64 bridge (TP1) Peninsula MANATEE RIVER Wingate CreekMine flows SE into Johnson Crk 27 28 24.14 82 12 39.4820020012OKLAWAHA RIVER AT SR316Peninsula OKLAWAHA RIVER No point sources orstormwater inputs upstream 29 22 24.34 81 54 6.3520020404ORANGE CREEK 50 YDS.UP FROM HWY. 21Peninsula ORANGE CREEKWater bottling facility (IWF) right at the sampling lat/longs.Subsequent additional information from DEP Bureau of Labs staffis that sampling trips in this area indicate that the IWF facility islocated downstream of this site. 29 30 33.44 81 56 47.820030253RICECR #3@BR SR#3/250yds above Bardine Peninsula RICE CREEK No point sources or stormwater inputs upstream 29 41 34 81 43 2720020317SILVER RUN ATCONFLUENCE WITHBOAT RAMP CANAL Peninsula SILVER RUNOne small WWTP (Sharpes Ferry Mobile Home Park) permittedfacility upstream, design capacity = 0.0190. Subsequentadditional information indicates the site is upstream of anydischarge.29 12 35.7 81 59 3628020221TELEGRAPH CREEK ATBABCOCK RANCH Peninsula TELEGRAPH CREEKOne large stormwater construction nearby (permitted 3/2005), butflows into Big Island Canal whichis also the receiving water forTelegraph Creek26 44 54.4 81 40 13.2102LatitudeLongitudeSTORETSite DescriptionBioregionWaterbody NameFacility Information from GIS CoveragesDegMinSecDegMinSec26011020TIGER CREEK ATWALKING WATERS RD. Peninsula TIGER CREEK No point sources or stormwater inputs upstream 27 48 43.53 81 26 38.6323010464Withlacoochee R @ countypark off Auton Rd (TP3)Peninsula WITHLACOOCHEE RIVERSmall stormwater construction permit for bike trail; permit startedin 4/200528 21 0 82 7 28.223010446 WITHLACOOCHEE RIVER Peninsula WITHLACOOCHEE RIVERNo point sources or stormwater inputs upstream; site belowWITHREF 28 18 13.9 82 4 0.1103Table J-2. Reference sites sample dataSTORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date20030245 Ates Creek @ CR 315Northeast BLACK CREEK 75.8 0 1.2 153 4/17/199720030437 BLACK CR., NORTH PRONG, 1/4 ABOVE BIG BRANCH Northeast BLACK CREEK56.1 0 1.2 103 8/1/199520030437 BLACK CR., NORTH PRONG, 1/4 ABOVE BIG BRANCH Northeast BLACK CREEK57.7 0 1.2 108 8/8/200020030437 BLACK CR., NORTH PRONG, 1/4 ABOVE BIG BRANCH Northeast BLACK CREEK66.9 0 1.2 117 8/8/200020030437 BLACK CR., NORTH PRONG, 1/4 ABOVE BIG BRANCH Northeast BLACK CREEK71.5 0 1.2 122 1/30/199620030437 BLACK CR., NORTH PRONG, 1/4 ABOVE BIG BRANCH Northeast BLACK CREEK67.3 0 1.2 123 7/20/199420030419 Black Creek, South ProngNortheast BLACK CREEK 68.4 0 1.4 103 1/24/199520030419 Black Creek, South ProngNortheast BLACK CREEK 64.3 0 1.4 106 7/20/199420030419 Black Creek, South ProngNortheast BLACK CREEK 79.3 0 1.4 106 3/20/199720030419 Black Creek, South ProngNortheast BLACK CREEK 66.1 0 1.4 119 7/11/200020030419 Black Creek, South ProngNortheast BLACK CREEK 81.3 0 1.4 150 7/7/199820030723 N. FORK BLACK CRK ABOVE CARTER SPENCERRD. Northeast BLACK CREEK43.4 0 1.2 108 7/12/199920030247 South Fork Black Creek @ SR 16Northeast BLACK CREEK90.2 0 1.1 123 4/17/199720030570 Bradley Crk @ 739B-Peter's crk basinNortheast BRADLEY CREEK 85.1 0 1.3 144 1/28/199820030487 Bull Creek @ NE Crossing of CR 215Northeast BULL CREEK63.9 0 1.2 164 4/17/199719010042 Calkins Ck, off Hamp Register Rd.Northeast CALKINS CREEK75.7 0 1.2 102 8/3/199419010042 Calkins Ck, off Hamp Register Rd.Northeast CALKINS CREEK80.4 0 1.2 108 11/1/199419010042 Calkins Ck, off Hamp Register Rd.Northeast CALKINS CREEK50.0 0 1.2 114 7/10/200121010043 Deep Creek upstream of bridge @Old RiverRdNortheast DEEP CREEK66.9 0 1.4 101 11/10/199721010018 Falling Creek @ CR 131, above falls SuwanneeR. Northeast FALLINGCREEK 51.5 0 1.4 112 7/12/199421010018 Falling Creek @ CR 131, above falls SuwanneeR. Northeast FALLINGCREEK 77.9 0 1.4 126 7/17/199721010018 Falling Creek @ CR 131, above falls SuwanneeR. Northeast FALLINGCREEK 39.0 0 1.4 132 7/27/199921010018 Falling Creek @ CR 131, above falls SuwanneeR. Northeast FALLINGCREEK 42.7 0 1.4 160 2/8/199421010018 Falling Creek @ CR 131, above falls SuwanneeR. Northeast FALLINGCREEK 87.8 0 1.4 161 1/4/199519010064 John Rowe Br, test site for NE FL State HospFYI Northeast JOHN ROWEBRANCH 62.7 0 1.8 142 11/1/199420030571 Peter's Crk above Rosemary hill Rd- Basin NortheastPETERSCREEK 89.001.2 110 1/26/1998104STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date19010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 78.801.3 105 3/7/199419010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 76.801.3 108 7/25/199419010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 78.901.3 109 3/6/199519010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 58.001.3 111 2/1/200019010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 83.501.3 156 3/3/199319010099 Pigeon Creek at US 1 St. Marys-Nassau River NortheastPIGEONCREEK 54.801.3 157 7/9/199621010056 PCS Phosphate FYI5, control siteNortheast ROBINSON CREEK79.2 0 1.4 113 3/13/200021010032 Rocky Ck @ Woodpecker RdNortheast ROCKY CREEK 56.7 0 1.2 152 8/24/199420030265 Sal Taylor Creek below Rowell CreekNortheast SAL TAYLOR CREEK 68.6 0 1.8 138 3/5/199620030265 Sal Taylor Creek below Rowell CreekNortheast SAL TAYLOR CREEK 35.1 0 1.8 146 10/2/199521030115 Santa Fe Rv- Control A BMPNortheast SANTA FE RIVER 75.7 0 1.5 148 11/6/199820030456 Thomas Ck, Forestry BMP Study Ref SiteBNortheast THOMAS CREEK75.7 0 1.2 118 7/2/199720030456 Thomas Ck, Forestry BMP Study Ref SiteBNortheast THOMAS CREEK82.6 0 1.2 128 2/12/199820030456 Thomas Ck, Forestry BMP Study Ref SiteBNortheast THOMAS CREEK92.4 0 1.2 129 3/14/199620030456 Thomas Ck, Forestry BMP Study Ref SiteBNortheast THOMAS CREEK86.7 0 1.2 159 2/21/199719010072 McClenny WWTP upstreamNortheast TURKEY CREEK 78.2 0 1.6 160 3/22/199920030341 Yellow Water Creek above Sal Taylor CreekNortheast YELLOW WATERCREEK 70.4 0 1.9 107 3/5/199620030341 Yellow Water Creek above Sal Taylor CreekNortheast YELLOW WATERCREEK 66.4 0 1.9 147 10/2/199520030342 Yellow Water Creek below Sal Taylor CreekNortheastYELLOWWATERCREEK 43.5 0 1.8 103 10/2/199520030342 Yellow Water Creek below Sal Taylor CreekNortheastYELLOWWATERCREEK 74.8 0 1.8 166 3/5/199622020070 BIG CYPRESS BRANCH-TIMBER ENERGY FYI CNTRL SITEPanhandle BIG CYPRESS BRANCH 53.6 0 1.3 125 8/9/199933040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 67.4 0 1.2 114 7/15/199633040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 41.7 0 1.2 119 2/17/199333040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 78.4 0 1.2 126 1/30/199533040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 75.3 0 1.2 148 2/8/200133040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 67.0 0 1.2 154 8/23/199433040014 BIG HORSE CREEK S.R.2 OKA.CO.Panhandle BIG HORSE CREEK 96.3 0 1.2 173 7/15/199821030064 Cedar Head Run, above Ichetucknee RiverPanhandle CEDAR HEADRUN 42.2 0 1.0 100 8/1/199421030064 Cedar Head Run, above Ichetucknee RiverPanhandle CEDAR HEADRUN 40.6 0 1.0 105 2/1/1995105STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date21030064 Cedar Head Run, above Ichetucknee RiverPanhandle CEDAR HEADRUN 46.2 0 1.0 108 2/6/199621030064 Cedar Head Run, above Ichetucknee RiverPanhandle CEDAR HEADRUN 69.4 0 1.0 111 3/15/200031010050 CROOKED CREEK HWY.270 GADSDEN CO.Panhandle CROOKED CREEK 71.1 0 1.1 108 2/13/199531010050 CROOKED CREEK HWY.270 GADSDEN CO.Panhandle CROOKED CREEK 58.5 0 1.1 132 3/25/199331010050 CROOKED CREEK HWY.270 GADSDEN CO.Panhandle CROOKED CREEK 84.8 0 1.1 151 7/9/199632030023 ECONFINA CREEK AT SCOTT RDPanhandle ECONFINA CREEK 68.2 0 1.2 102 2/7/199532030023 ECONFINA CREEK AT SCOTT RDPanhandle ECONFINA CREEK 70.4 0 1.2 106 8/25/199232030023 ECONFINA CREEK AT SCOTT RDPanhandle ECONFINA CREEK 62.3 0 1.2 124 2/6/200132030023 ECONFINA CREEK AT SCOTT RDPanhandle ECONFINA CREEK 81.3 0 1.2 151 6/28/199332030023 ECONFINA CREEK AT SCOTT RDPanhandle ECONFINA CREEK 87.9 0 1.2 162 2/5/199722050077 Econfina River Forestry BMP Study Ref SiteB PanhandleECONFINARIVER 59.8 0 1.1 135 5/13/199833040064 Jacks Branch, Forestry BMP Study Ref SiteB Panhandle JACK'S BRANCH 63.4 0 1.7 111 2/22/199633040064 Jacks Branch, Forestry BMP Study Ref SiteB Panhandle JACK'S BRANCH 63.6 0 1.7 140 2/25/199733040064 Jacks Branch, Forestry BMP Study Ref SiteB Panhandle JACK'S BRANCH 66.9 0 1.7 173 7/1/199832010024 Limestone Ck @ Beckridge RdPanhandle LIMESTONE CREEK 74.5 0 1.3 171 7/14/199832020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 78.6 0 1.2 100 1/21/199932020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 67.4 0 1.2 113 2/9/199532020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 62.3 0 1.2 116 8/30/199432020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 71.5 0 1.2 126 6/29/199332020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 72.6 0 1.2 129 2/23/199432020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 80.1 0 1.2 153 7/16/199632020063 LITTLE CROOKED CREEK S.R.79 BAY CO.Panhandle LTL CROOKED CREEK 52.7 0 1.2 156 3/1/199322030062 MCBRIDE SLOUGH HWY.267 WAKULLA CO.Panhandle MCBRIDE SLOUGH 41.6 0 1.0 112 7/8/199222030062 MCBRIDE SLOUGH HWY.267 WAKULLA CO.Panhandle MCBRIDE SLOUGH 60.2 0 1.0 138 3/21/199422030062 MCBRIDE SLOUGH HWY.267 WAKULLA CO.Panhandle MCBRIDE SLOUGH 57.3 0 1.0 147 2/11/199733010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 73.20 1.3 101 1/24/199533010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 75.60 1.3 115 2/22/200133010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 68.70 1.3 118 2/18/199333010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 48.50 1.3 126 8/10/1994106STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date33010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 70.20 1.3 127 8/12/199733010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 76.90 1.3 141 8/17/199233010054 MCDAVID CREEK AT SR 99Panhandle MCDAVID CREEK 88.70 1.3 173 2/14/199422020049 MULE CREEK S.R.12 LIBERTY CO.Panhandle MULE CREEK49.9 0 1.2 104 3/25/199322020049 MULE CREEK S.R.12 LIBERTY CO.Panhandle MULE CREEK65.7 0 1.2 106 2/13/199522020049 MULE CREEK S.R.12 LIBERTY CO.Panhandle MULE CREEK57.7 0 1.2 112 2/23/199922020049 MULE CREEK S.R.12 LIBERTY CO.Panhandle MULE CREEK58.6 0 1.2 114 7/8/199222020049 MULE CREEK S.R.12 LIBERTY CO.Panhandle MULE CREEK67.8 0 1.2 125 7/9/199622020062 Oklawaha Ck, SR 267, S of QuincyPanhandle OKLAWAHA CREEK52.0 0 1.1 100 9/9/199822020062 Oklawaha Ck, SR 267, S of QuincyPanhandle OKLAWAHA CREEK41.8 0 1.1 101 8/1/199422020062 Oklawaha Ck, SR 267, S of QuincyPanhandle OKLAWAHA CREEK45.2 0 1.1 105 8/12/199722020062 Oklawaha Ck, SR 267, S of QuincyPanhandle OKLAWAHA CREEK48.3 0 1.1 108 2/13/199522020062 Oklawaha Ck, SR 267, S of QuincyPanhandle OKLAWAHA CREEK82.9 0 1.1 147 2/13/199631020073 Pitts Mill Creek above conf with Cypress CkPanhandle PITTS MILL CREEK 68.2 0 1.5 115 7/23/199622020010 QUINCY CR above SR12, ref for Quincy STPFYI Panhandle QUINCYCREEK 45.5 0 1.5 105 11/6/199522030010 St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle ST. MARKS RIVER 40.5 0 1.3 100 9/18/200122030010 St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle ST. MARKS RIVER 56.0 0 1.3 105 3/18/199722030010 St. Marks R approx 4 mi N of Hwy 98 (@ "rapids") Panhandle ST. MARKS RIVER 46.8 0 1.3 108 8/22/200031010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 69.9 0 1.3 104 2/13/199631010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 75.2 0 1.3 110 3/21/199431010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 97.8 0 1.3 110 7/8/199231010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 59.7 0 1.3 129 8/1/199431010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 66.7 0 1.3 132 3/25/199331010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 70.0 0 1.3 151 2/13/199531010051 SWEETWATER CREEK HWY.270 LIBERTY CO.Panhandle SWEETWATER CREEK 84.1 0 1.3 162 7/20/199331020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK50.5 0 1.2 107 8/26/199431020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK72.9 0 1.2 113 8/20/199231020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK82.0 0 1.2 124 2/7/199531020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK55.3 0 1.2 125 2/23/1993107STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date31020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK76.1 0 1.2 126 2/21/199431020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK62.0 0 1.2 129 6/24/199331020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK67.7 0 1.2 130 2/4/199731020040 TEN MILE CREEK S.R.73 CALHOUN CO.Panhandle TEN MILE CREEK49.4 0 1.2 141 2/10/199833010065 Rest Area Run Creek below I 10Panhandle UNNAMED CREEK 82.2 0 1.2 113 7/12/199533010065 Rest Area Run Creek below I 10Panhandle UNNAMED CREEK 54.7 0 1.2 147 2/14/199622040038 Unnamed Creek above Monticello WWTPPanhandle UNNAMED CREEK 54.7 0 1.7 153 12/12/199532020066 Wright Ck, ref for Noma STP FYIPanhandle WRIGHTS CREEK 73.9 0 1.7 123 4/29/199320010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 59.0 0 1.0 109 3/29/199420010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 56.5 0 1.0 111 7/19/199420010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 80.9 0 1.0 126 8/26/199620010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 54.0 0 1.0 127 9/28/199320010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 51.9 0 1.0 128 8/11/199220010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 59.7 0 1.0 133 2/8/199320010455 BLACKWATER CREEK AT HWY 44APeninsula BLACKWATER CREEK 33.5 0 1.0 143 2/7/199528020239 Cypress brch above SR 78Peninsula CYPRESS BRANCH 57.6 0 1.3 135 8/20/199728020239 Cypress brch above SR 78Peninsula CYPRESS BRANCH 70.3 0 1.3 142 8/20/199728020237 Cypress Creek 2 mi above SR78Peninsula CYPRESS CREEK 55.7 0 1.2 132 7/21/199928020237 Cypress Creek 2 mi above SR78Peninsula CYPRESS CREEK 70.4 0 1.2 135 8/5/199726010972 DAVENPORT CR AT SR 545 BRIDGEPeninsula DAVENPORT CREEK63.0 0 2.0 104 8/16/199426010972 DAVENPORT CR AT SR 545 BRIDGEPeninsula DAVENPORT CREEK66.3 0 2.0 110 9/30/199726010972 DAVENPORT CR AT SR 545 BRIDGEPeninsula DAVENPORT CREEK73.6 0 2.0 113 2/14/199526010972 DAVENPORT CR AT SR 545 BRIDGEPeninsula DAVENPORT CREEK79.4 0 2.0 167 1/10/200026010593 FISHEATING CREEK ABOVE US 27Peninsula FISHEATING CREEK 60.8 0 1.1 113 3/9/199320030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 90.0 0 1.0 100 8/21/199620030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 72.2 0 1.0 102 1/24/199520030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 80.8 0 1.0 109 7/20/199420030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 100.0 0 1.0 111 1/12/200020030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 89.2 0 1.0 121 3/10/1998108STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date20030414 GOLD HEAD BR. IN STATE PARKPeninsula GOLD HEAD BRANCH 77.4 0 1.0 123 2/15/199424030142 Hillsborough R upstrm of Southside Br, ZephyrhilPeninsula HILLSBOROUGH RIVER 87.10 1.6 145 8/21/199624030048 Hillsborough River at SR 39Peninsula HILLSBOROUGH RIVER60.8 0 1.5 109 8/21/199625020111 Horse Creek @ SR 72 BridgePeninsula HORSE CREEK 70.4 0 1.8 110 2/17/199925020111 Horse Creek @ SR 72 BridgePeninsula HORSE CREEK 66.60 1.8 147 10/24/199420010454 JUNIPER CREEK AT HIGHWAY 19Peninsula JUNIPER CREEK 55.8 0 1.1 110 1/31/199520010454 JUNIPER CREEK AT HIGHWAY 19Peninsula JUNIPER CREEK 29.6 0 1.1 115 8/3/199320010454 JUNIPER CREEK AT HIGHWAY 19Peninsula JUNIPER CREEK 75.5 0 1.1 146 3/4/199620020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 73.2 0 1.2 133 2/9/199820020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 82.5 0 1.2 134 2/12/199620020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 86.8 0 1.2 138 8/30/199420020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 82.2 0 1.2 139 2/15/199420020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 90.3 0 1.2 150 9/26/199520020004 Little Orange Creek @ Hwy 21Peninsula LITTLE ORANGE CREEK 75.3 0 1.2 161 8/15/200026011019 LIVINGSTON CREEK AT RUCKS DAIRY RD.Peninsula LIVINGSTON CREEK 71.2 0 1.5 118 9/5/200126011019 LIVINGSTON CREEK AT RUCKS DAIRY RD.Peninsula LIVINGSTON CREEK 61.5 0 1.5 156 2/18/199728010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER34.3 0 1.0 106 9/21/199828010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER49.2 0 1.0 115 9/22/199528010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER59.9 0 1.0 124 10/7/199928010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER33.9 0 1.0 134 8/20/199228010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER54.8 0 1.0 146 8/25/199428010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER33.8 0 1.0 158 3/22/199928010223 N FK LOXAHATCHEE RIVER REF BIO STATIONPeninsula LOXAHATCHEE RIVER44.7 0 1.0 161 2/20/199624010002 Manatee R 20 m below SR64 bridge (TP1)Peninsula MANATEE RIVER60.3 0 1.3 109 3/17/199324010002 Manatee R 20 m below SR64 bridge (TP1)Peninsula MANATEE RIVER76.3 0 1.3 126 2/2/199424010002 Manatee R 20 m below SR64 bridge (TP1)Peninsula MANATEE RIVER77.2 0 1.3 129 7/17/199624010002 Manatee R 20 m below SR64 bridge (TP1)Peninsula MANATEE RIVER96.7 0 1.3 145 2/2/199424010002 Manatee R 20 m below SR64 bridge (TP1)Peninsula MANATEE RIVER80.2 0 1.3 172 11/21/200020020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 77.9 0 1.0 101 8/29/1995109STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date20020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 65.2 0 1.0 117 3/20/199520020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 67.2 0 1.0 117 8/10/199320020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 68.1 0 1.0 137 2/15/200020020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 80.6 0 1.0 151 8/11/199820020012 OKLAWAHA RIVER AT SR 316Peninsula OKLAWAHA RIVER 49.6 0 1.0 153 2/8/199320020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 93.1 0 1.4 105 8/17/199320020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 94.5 0 1.4 110 9/22/199220020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 92.1 0 1.4 111 8/30/199420020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 83.1 0 1.4 118 3/16/199320020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 91.8 0 1.4 135 8/15/200020020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 94.3 0 1.4 139 2/15/199420020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 72.0 0 1.4 144 8/15/200020020404 ORANGE CREEK 50 YDS. UP FROM HWY. 21Peninsula ORANGE CREEK 96.3 0 1.4 168 2/28/199520030253 RICECR #3@BR SR #3/250yds above Bardine Peninsula RICE CREEK67.1 0 1.1 125 5/11/199920030253 RICECR #3@BR SR #3/250yds above Bardine Peninsula RICE CREEK24.0 0 1.1 136 3/16/199820020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 34.801.3 133 9/1/199220020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 42.301.3 136 8/10/199320020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 53.501.3 142 9/4/200120020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 57.601.3 142 2/8/199420020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 54.201.3 150 3/20/199520020317 SILVER RUN AT CONFLUENCE WITH BOAT RAMPCANAL Peninsula SILVERRUN 76.001.3 166 9/16/199628020221 TELEGRAPH CREEK AT BABCOCK RANCHPeninsula TELEGRAPH CREEK 84.4 0 1.0 107 9/16/199628020221 TELEGRAPH CREEK AT BABCOCK RANCHPeninsula TELEGRAPH CREEK 80.8 0 1.0 146 7/27/199426011020 TIGER CREEK AT WALKING WATERS RD.Peninsula TIGER CREEK74.4 0 1.0 109 7/14/199226011020 TIGER CREEK AT WALKING WATERS RD.Peninsula TIGER CREEK76.5 0 1.0 111 2/18/199726011020 TIGER CREEK AT WALKING WATERS RD.Peninsula TIGER CREEK75.7 0 1.0 142 2/21/199526011020 TIGER CREEK AT WALKING WATERS RD.Peninsula TIGER CREEK73.4 0 1.0 161 7/20/199923010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 43.4 0 1.2 100 9/28/199423010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 46.9 0 1.2 100 9/2/1993110STORETSite DescriptionBioregionWaterbody NameSCIHDGLDITotal # individualscountedSample Date23010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 67.8 0 1.2 120 3/9/199923010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 50.6 0 1.2 122 7/26/199923010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 49.6 0 1.2 125 3/11/199323010464 Withlacoochee R @ county park off Auton Rd (TP3) Peninsula WITHLACOOCHEERIVER 66.1 0 1.2 136 2/13/199623010446 WITHLACOOCHEE RIVERPeninsula WITHLACOOCHEE RIVER 50.0 0 1.6 147 7/28/1999Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A7 -1 

## Appendix A7. FDEP’s Site Information and Taxa Lists for SCI BCG Workshop Samples

Cross

- references:
Appendix B-16: FDEP’s Site Information and Taxa Lists for SCI BCG Workshop Samples . U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule . January 14, 2010. EPA- HQ

- OW -2009- 0596, FRL-9105-1.
Appendix 3-E . U.S. EPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters. United States Environmental Protection Agency, Office of Water, Office of Science and Technology, Health and Ecological Criteria Division, Washington, DC.

# FDEP’s Site Info rmation and Taxa Lists for SCI BCG Workshop Samples

(Appendix 3-E from USEPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters . United States Environm ental Protection Agency, Office of Water, Office of Science and Technol ogy, Health and Ecological Criteria Division, Washington, DC.)If you have a disability and are unable to read this spreadsheet, please contact Eric Shaw at Eric.Shaw@dep.state.fl.us, and you will be provided with an accessible version.BCGSampleID STORET Station Nickname Bioregion Sample Date SCI HDG LDI Selection Rationale NE01 19020027 ALIGTRREF Northeast 4/11/2001 47.1 1 1.4 SCI [35-50] random NE02 19010049 BLDWIN REF Northeast 2/14/2000 32.9 2 3.22 Random NE03 19020053 CALAHANTST Northeast 9/24/2001 24.4 3 1.41 SCI 10th Percentile (22.96) NE04 19020053 CALAHANTST Northeast 7/15/1996 40.1 3 1.41 SCI [35-50] random NE05 20030549 CECFLD6 Northeast 3/6/1996 73.6 1 2 LDI [2-9] random NE06 20030332 JAXHEITTST Northeast 5/5/1992 28.9 4 6.11 HDG [3-5] random NE07 21030059 LKBUTTST Northeast 9/15/1992 9.1 3 5.26 LDI [2-9] random NE08 19010073 MCCLNYTST Northeast 3/22/1999 68 0 1.55 SCI 75th Percentile (68.27) NE09 20030573 MILOG209 Northeast 1/26/1998 30.6 1 1.61 SCI 25th Percentile (30.39) NE10 19020059 YULEEREF Northeast 10/28/1996 47 0 1.28 SCI 50th Percentile (45.09) Panh01 22020078 A BHOPTST Panhandle 6/4/1996 20.7 3 1 HDG [3-5] random Panh02 33040073 CANEYCBELL Panhandle 8/22/2000 30 3 1.71 HDG [3-5] random Panh03 31010125 CHATTATST Panhandle 10/13/1998 62.5 0 1.62 SCI 50th Percentile (62.40) Panh04 31020071 CHTMDLCRKD Panhandle 7/23/1996 70.5 1 1.72 SCI 75th Percentile (70.50) Panh05 31020072 CHTMDLOTTR Panhandle 7/23/1996 43.5 2 1.42 Random Panh06 33010112 COFECSTLR Panhandle 2/16/1999 47.7 2 1.05 SCI [35-50] random Panh07 31020042 DOZREF Panhandle 9/1/1992 30.2 6 2.83 SCI 25th Percentile (30.10) Panh08 31020043 DOZTST Panhandle 9/1/1992 35.8 6 2.75 SCI [35-50] random Panh09 22030064 FLAMINREF Panhandle 3/8/1993 4.6 7 8.08 SCI 10th Percentile (4.92) Panh10 22050003 TAYLO4 Panhandle 4/10/2000 0.6 3 2.28 HDG [3-5] random Pen01 20030585 BUNCNTRL Peninsula 9/8/1998 15.5 6 5.29 HDG [6-9] random Pen02 25020550 DESOT34 Peninsula 9/3/1997 37.5 4 2.91 SCI [35-50] random Pen03 27010580 GROOVRARPT Peninsula 8/22/2000 24.2 1 1.79 SCI 25th Percentile (24.55) Pen04 24040117 GULFTST Peninsula 10/8/1998 14.8 7 7.85 SCI 10th Percentile (14.45) Pen05 24030044 HILSTP5REF Peninsula 8/18/1993 55 1 2.12 HDG [1-2] random Pen06 20010384 LONGBRANCH Peninsula 7/13/1999 38.2 3 2.53 SCI [35-50] random Pen07 28010224 NWFRLOXREF Peninsula 9/20/1995 40.8 4 3.87 SCI 50th Percentile (40.81) Pen08 25020014 OAKREF Peninsula 8/22/1995 58.3 1 2.1 SCI 75th Percentile (58.70) Pen09 20020145 SWEETC K Peninsula 8/10/1998 21.1 2 1.3 Random Pen10 20020420 WGCITREF Peninsula 12/1/1992 0 7 8.32 HDG [6-9] randomBCGSampleID NERef01 NERef02 NERef03 NERef04 NERef05 Functional Feeding Groups % Browser-Grazers 0.93 0000 % Burrowing Deposit Feeders 1.87 0 0.9 1.77 3.88 % Parasites 00000 % Plant Piercers 00000 % Predators 9.81 10.98 13.96 15.93 15.12 % Scavengers 1.4 3.79 7.21 3.54 0.78 % Scrapers 12.62 45.45 22.52 11.06 8.91 % Shredders 12.15 11.36 13.06 2.65 14.34 % Surface Deposit Feeders 35.98 23.86 31.98 25.22 31.01 % Suspension Feeders 18.69 4.55 6.76 39.82 24.42 % Unknown 6.54 0 3.6 0 1.55

Taxonomic Composition % Amphipoda 0 0.76 13.51 0 2.33 % Chironomidae 11.21 25 28.83 47.79 31.01 % Coleoptera 0.49 7.58 8.11 5.31 3.88 % Decapoda 2.8 0000 % Diptera 25.23 25.76 29.73 53.1 51.16 % Dominant Taxon 12.15 28.79 13.51 18.58 10.85 % Ephemeroptera 27.1 18.18 16.22 15.04 11.63 % Gastropoda 6.54 32.58 12.61 2.65 1.55 % Isopoda 0 3.79 7.21 3.54 0.78 % Odonata 0.93 3.79 2.7 0.88 2.33 % Oligochaeta 1.87 0 0.9 1.77 3.88 % Pelecypoda 12.15 3.03 3.6 0.88 0 % Plecoptera 0 0 0 2.65 8.53 % Tanytarsini individuals 0 0 2.7 27.43 6.2 % Very Tolerant individuals 0.93 29.55 11.71 7.96 0.78 % Trichoptera 5.61 4.55 1.8 13.27 10.85 % Trombidiformes 1.86 0 3.6 0 2.33 Number of Chironomid taxa 7 8 10 13 14 Number of Clinger Taxa 5 3 6 7 10 Number of Ephemeroptera taxa 64543 Number of EPT taxa 9 6 6 7 10 Number of Long-Lived Taxa 20015 Number of Oligochaete taxa 10123 Number of Plecoptera Taxa 00012 Number of Sensitive Taxa 9 6 10 10 16 Number of Trichoptera taxa 32125 Total Number of Individuals 107 132 111 113 129 Total Number of Taxa 34 25 33 35 43 Northeast Bioregion Reference Sites

FDEP BCG Workshop, 10/24/06BCGSampleID PanhRef01 PanhRef02 PanhRef03 PanhRef04 PanhRef05 Functional Feeding Groups % Browser-Grazers 0 0 0.34 1 0 % Burrowing Deposit Feeders 0 1.17 2.04 1 0.91 % Parasites 00000 % Plant Piercers 0000 0.91 % Predators 6.17 11.99 15.99 19.5 22.27 % Scavengers 0 0.29 1.36 7 2.73 % Scrapers 21.1 24.56 4.76 24.5 8.18 % Shredders 15.58 6.14 4.76 14 11.36 % Surface Deposit Feeders 40.91 40.64 17.35 30.5 22.27 % Suspension Feeders 16.23 14.62 53.4 2.5 31.36 % Unknown 0 0.58 0 0 0 Taxonomic Composition % Amphipoda 0 5.26 0 11 0 % Chironomidae 33.12 16.37 30.61 16 29.09 % Coleoptera 18.18 20.47 3.4 12 10 % Decapoda 0 0.58 1.36 6 4.55 % Diptera 44.81 25.15 79.59 16 31.82 % Dominant Taxon 9.74 16.96 47.62 11 19.09 % Ephemeroptera 24.03 33.33 6.12 11 13.64 % Gastropoda 0 0 0 18 0.91 % Isopoda 0 0 0.68 4 0 % Odonata 0 0 1.36 6 2.73 % Oligochaeta 0 1.17 2.04 1 0.91 % Pelecypoda 0 0.58 2.04 0 0 % Plecoptera 0.65 1.75 0.68 0 8.18 % Tanytarsini individuals 2.6 7.02 6.8 4 20 % Trichoptera 9.74 9.36 0.68 2 22.73 % Trombidiformes 1.95 0.58 2.04 11 1.82 % Very Tolerant individuals 9.09 1.17 4.08 14 0.91 Number of Chironomid taxa 12 14 14 4 10 Number of Clinger Taxa 12 11 6 2 16 Number of Ephemeroptera taxa 75236 Number of EPT taxa 13 13 4 5 19 Number of Long-Lived Taxa 23439 Number of Oligochaete taxa 01211 Number of Plecoptera taxa 11104 Number of Sensitive Taxa 15 14 11 4 14 Number of Trichoptera taxa 57129 Total Number of Individuals 154 171 147 100 110 Total Number of Taxa 38 41 35 28 46 Panhandle Bioregion Reference Sites

FDEP BCG Workshop, 10/24/06BCGSampleID PenRef01 PenRef02 PenRef03 PenRef04 PenRef05 Functional Feeding Groups % Browser-Grazers 0 0 0 1.46 0 % Burrowing Deposit Feeders 0 4.5 4.65 5.84 1.47 % Parasites 2.27 0000 % Plant Piercers 0000 0.74 % Predators 5.3 17.57 4.36 6.57 4.41 % Scavengers 0 0 0.58 1.46 0 % Scrapers 40.15 12.16 18.31 9.85 5.51 % Shredders 7.95 8.56 13.95 20.07 32.35 % Surface Deposit Feeders 29.92 21.62 32.56 35.04 45.59 % Suspension Feeders 14.39 30.18 25.58 19.71 9.93 % Unknown 0 5.41 0 0 0 Taxonomic Composition % Amphipoda 1.52 0 0 20.44 1.47 % Chironomidae 15.15 37.84 43.6 36.5 68.38 % Coleoptera 10.61 1.8 23.26 5.84 14.71 % Decapoda 0 0 1.16 2.92 0 % Diptera 23.48 41.44 49.42 43.07 69.85 % Dominant Taxon 20.45 9.91 24.42 20.44 59.56 % Ephemeroptera 29.55 13.51 11.05 10.22 1.47 % Gastropoda 20.45 2.7 0 2.19 0 % Isopoda 00000 % Odonata 3.03 4.5 0.58 0.73 0 % Oligochaeta 0 4.5 4.65 5.84 1.47 % Pelecypoda 3.79 0 1.74 0.73 4.41 % Plecoptera 0 0.9 0 0 0 % Tanytarsini individuals 1.52 18.92 15.7 14.6 2.94 % Trichoptera 3.79 28.83 6.98 6.57 1.47 % Trombidiformes 0.76 1.8 0.58 0 4.42 % Very Tolerant individuals 25.76 0.9 1.74 8.03 61.03 Number of Chironomid taxa 7 14 10 10 6 Number of Clinger Taxa 5 12 8 6 4 Number of Ephemeroptera taxa 43331 Number of EPT taxa 7 14 9 7 2 Number of Long-Lived Taxa 23220 Number of Oligochaete taxa 03232 Number of Plecoptera taxa 01000 Number of Sensitive Taxa 4 11 6 5 3 Number of Trichoptera taxa 3 10 6 4 1 Total Number of Individuals 132 111 172 137 136 Total Number of Taxa 29 40 30 34 23 Peninsula Bioregion Reference Sites

FDEP BCG Workshop, 10/24/06If you have a disability and are unable to read this spreadsheet, please contact Eric Shaw at Eric.Shaw@dep.state.fl.us, and you will be provided with an accessible version.

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Caecidotea 39 Isopoda Asellidae Micromenetus 13 Basommatophora Planorbidae Caenis 13 Ephemeroptera Caenidae Elliptio 12 Unionoida Unionidae Hyalella azteca 11 Amphipoda Hyalellidae Simulium 9 Diptera Simuliidae Ancylidae 7 Basommatophora Rheotanytarsus exiguus grp. 4 Diptera Chironomidae Cheumatopsyche 4 Trichoptera Hydropsychidae Stenelmis 4 Coleoptera Elmidae Ancyronyx variegatus 2 Coleoptera Elmidae Ablabesmyia mallochi 2 Diptera Chironomidae Tribelos jucundum 2 Diptera Chironomidae Polypedilum illinoense grp. 2 Diptera Chironomidae Labrundinia pilosella 1 Diptera Chironomidae Dicrotendipes simpsoni 1 Diptera Chironomidae Baetidae 1 Ephemeroptera Nanocladius 1 Diptera Chironomidae Atrichopogon 1 Diptera Ceratopogonidae Hydrobiidae 1 Mesogastropoda Stenochironomus 1 Diptera Chironomidae Clinotanypus 1 Diptera Chironomidae Procambarus 1 Decapoda Cambaridae Eclipidrilus 1 Lumbriculida Lumbriculidae Polypedilum scalaenum grp. 1 Diptera Chironomidae Northeast NE01

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Pelecypoda 26 Hebetancylus excentricus 12 Basommatophora Ancylidae Paratanytarsus dissimilis 12 Diptera Chironomidae Tanytarsus sp. g epler 10 Diptera Chironomidae Tanytarsus sp. l epler 6 Diptera Chironomidae Polypedilum tritum 5 Diptera Chironomidae Dero digitata complex 3 Haplotaxida Naididae Crangonyx 3 Amphipoda Crangonyctidae Polypedilum illinoense grp. 3 Diptera Chironomidae Neoporus 3 Coleoptera Dytiscidae Kiefferulus 3 Diptera Chironomidae Physella 2 Basommatophora Physidae Tanypus 2 Diptera Chironomidae Haber speciosus 2 Haplotaxida Tubificidae Larsia 2 Diptera Chironomidae Clinohelea 1 Diptera Ceratopogonidae Corynoneura 1 Diptera Chironomidae Ablabesmyia mallochi 1 Diptera Chironomidae Tanytarsus sp. a epler 1 Diptera Chironomidae Limnodrilus hoffmeisteri 1 Haplotaxida Tubificidae Tribelos 1 Diptera Chironomidae Nanocladius 1 Diptera Chironomidae 

### Northeast NE02

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Polypedilum illinoense grp. 50 Diptera Chironomidae Limnodrilus hoffmeisteri 13 Haplotaxida Tubificidae Sphaeriidae 8 Veneroida Aulodrilus pigueti 6 Haplotaxida Tubificidae Chironomus 4 Diptera Chironomidae Pseudocloeon propinquum 4 Ephemeroptera Baetidae Dero 2 Haplotaxida Naididae Ancyronyx variegatus 2 Coleoptera Elmidae Allonais paraguayensis 2 Haplotaxida Naididae Simulium 2 Diptera Simuliidae Haber speciosus 2 Haplotaxida Tubificidae Enchytraeidae 1 Haplotaxida Psammoryctides convolutus 1 Haplotaxida Tubificidae Polypedilum halterale grp. 1 Diptera Chironomidae Stenacron floridense 1 Ephemeroptera Heptageniidae Hydrobiidae 1 Mesogastropoda Eclipidrilus palustris 1 Lumbriculida Lumbriculidae Tanytarsus 1 Diptera Chironomidae Calopteryx 1 Odonata Calopterygidae Caenis 1 Ephemeroptera Caenidae Coenagrionidae 1 Odonata Ancylidae 1 Basommatophora 

### Northeast NE03

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Polypedilum illinoense grp. 20 Diptera Chironomidae Pisidiidae 18 Veneroida Micromenetus 13 Basommatophora Planorbidae Amnicola 10 Mesogastropoda Bithyniidae Enallagma signatum 7 Odonata Coenagrionidae Nanocladius 6 Diptera Chironomidae Caenis 6 Ephemeroptera Caenidae Limnodrilus hoffmeisteri 6 Haplotaxida Tubificidae Pentaneura inconspicua 5 Diptera Chironomidae Ablabesmyia rhamphe grp. 5 Diptera Chironomidae Argia 5 Odonata Coenagrionidae Physella 4 Basommatophora Physidae Tanytarsus sp. a epler 4 Diptera Chironomidae Baetis 4 Ephemeroptera Baetidae Parachironomus carinatus 3 Diptera Chironomidae Dero trifida 3 Haplotaxida Naididae Microcylloepus pusillus 2 Coleoptera Elmidae Hyalella azteca 1 Amphipoda Hyalellidae Dubiraphia vittata 1 Coleoptera Elmidae Stenelmis 1 Coleoptera Elmidae Ancyronyx variegatus 1 Coleoptera Elmidae Prionocyphon 1 Coleoptera norowsselected Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Polypedilum convictum grp. 1 Diptera Chironomidae Labrundinia pilosella 1 Diptera Chironomidae Stenochironomus 1 Diptera Chironomidae Simulium 1 Diptera Simuliidae Tabanidae 1 Diptera Allonais paraguayensis 1 Haplotaxida Naididae Stylaria lacustris 1 Haplotaxida Naididae Caecidotea 1 Isopoda Asellidae Calopteryx 1 Odonata Calopterygidae Corduliidae 1 Odonata 

### Northeast NE04

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Stenelmis 19 Coleoptera Elmidae Ancylidae 11 Basommatophora Oecetis 9 Trichoptera Leptoceridae Polypedilum illinoense 8 Diptera Chironomidae Stenacron interpunctatum 6 Ephemeroptera Heptageniidae Eurylophella temporalis 5 Ephemeroptera Ephemerellidae Stempellinella 5 Diptera Chironomidae Cladotanytarsus 4 Diptera Chironomidae Caenis 4 Ephemeroptera Caenidae Polypedilum scalaenum grp. 3 Diptera Chironomidae Stenonema smithae 2 Ephemeroptera Heptageniidae Tanytarsus sp. t epler 2 Diptera Chironomidae Amnicola 2 Mesogastropoda Bithyniidae Stenochironomus 2 Diptera Chironomidae Paraleptophlebia volitans 1 Ephemeroptera Leptophlebiidae Palaemonetes paludosus 1 Decapoda Palaemonidae Pisidiidae 1 Veneroida Micromenetus dilatatus 1 Basommatophora Planorbidae Hemerodromia 1 Diptera Empididae Labrundinia 1 Diptera Chironomidae Conchapelopia 1 Diptera Chironomidae Simulium 1 Diptera Simuliidae Triaenodes 1 Trichoptera Leptoceridae Cheumatopsyche 1 Trichoptera Hydropsychidae Lype 1 Trichoptera Psychomyiidae Prionocyphon 1 Coleoptera Sminthurides 1 Collembola Sminthuridae Tanytarsus sp. c epler 1 Diptera Chironomidae Tanytarsus sp. o epler 1 Diptera Chironomidae Ablabesmyia rhamphe grp. 1 Diptera Chironomidae Ancyronyx variegatus 1 Coleoptera Elmidae Pentaneura inconspicua 1 Diptera Chironomidae Tribelos jucundum 1 Diptera Chironomidae Rheocricotopus robacki 1 Diptera Chironomidae 

### Northeast NE05

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Enallagma cardenium 24 Odonata Coenagrionidae Lumbriculus inconstans 15 Lumbriculida Lumbriculidae Polypedilum scalaenum grp. 13 Diptera Chironomidae Physella 9 Basommatophora Physidae Polypedilum tritum 9 Diptera Chironomidae Chironomus decorus 7 Diptera Chironomidae Pisidiidae 4 Veneroida Dicrotendipes neomodestus 3 Diptera Chironomidae Ischnura 3 Odonata Coenagrionidae Cheumatopsyche 3 Trichoptera Hydropsychidae Argia sedula 3 Odonata Coenagrionidae Hemerodromia 3 Diptera Empididae Conchapelopia 2 Diptera Chironomidae Stenelmis 2 Coleoptera Elmidae Progomphus obscurus 1 Odonata Gomphidae Tipulidae 1 Diptera Tanytarsus sp. e epler 1 Diptera Chironomidae Peltodytes muticus 1 Coleoptera Haliplidae Dicrotendipes modestus 1 Diptera Chironomidae Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Mesovelia 1 Heteroptera Mesoveliidae Abedus 1 Heteroptera Belostomatidae Hydropsyche 1 Trichoptera Hydropsychidae Ablabesmyia mallochi 1 Diptera Chironomidae 

### Northeast NE06

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Polypedilum illinoense grp. 62 Diptera Chironomidae Physella 32 Basommatophora Physidae Limnodrilus hoffmeisteri 23 Haplotaxida Tubificidae Chironomus 23 Diptera Chironomidae Enchytraeidae 7 Haplotaxida Lirceus 2 Isopoda Asellidae Stenelmis 2 Coleoptera Elmidae Paratanytarsus 1 Diptera Chironomidae Ischnura 1 Odonata Coenagrionidae Cryptochironomus 1 Diptera Chironomidae Pseudorthocladius 1 Diptera Chironomidae Macronychus glabratus 1 Coleoptera Elmidae 

### Northeast NE07

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Tanytarsus sp. m epler 29 Diptera Chironomidae Polypedilum convictum grp. 25 Diptera Chironomidae Cheumatopsyche 14 Trichoptera Hydropsychidae Rheocricotopus robacki 13 Diptera Chironomidae Ablabesmyia mallochi 10 Diptera Chironomidae Caecidotea 8 Isopoda Asellidae Ablabesmyia rhamphe grp. 7 Diptera Chironomidae Stenelmis 5 Coleoptera Elmidae Simulium 4 Diptera Simuliidae Rheotanytarsus exiguus grp. 4 Diptera Chironomidae Tanytarsus sp. u epler 3 Diptera Chironomidae Ancylidae 3 Basommatophora Polypedilum illinoense grp. 3 Diptera Chironomidae Polypedilum scalaenum grp. 3 Diptera Chironomidae Thienemannimyia grp. 3 Diptera Chironomidae Rheotanytarsus distinctissimus grp. 2 Diptera Chironomidae Tanytarsus sp. l epler 2 Diptera Chironomidae Procladius 2 Diptera Chironomidae Tanytarsus sp. c epler 1 Diptera Chironomidae Microcylloepus pusillus 1 Coleoptera Elmidae Stenonema smithae 1 Ephemeroptera Heptageniidae Slavina appendiculata 1 Haplotaxida Naididae Cladotanytarsus 1 Diptera Chironomidae Cryptotendipes 1 Diptera Chironomidae Conchapelopia 1 Diptera Chironomidae Enallagma 1 Odonata Coenagrionidae Progomphus 1 Odonata Gomphidae Acerpenna pygmaea 1 Ephemeroptera Baetidae Xenochironomus xenolabis 1 Diptera Chironomidae Thienemanniella xena 1 Diptera Chironomidae Hygrobates 1 Acariformes Hygrobatidae Gyrinidae 1 Coleoptera Cricotopus bicinctus 1 Diptera Chironomidae Pentaneura inconspicua 1 Diptera Chironomidae 

### Northeast NE08

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Cheumatopsyche 57 Trichoptera Hydropsychidae Simulium 17 Diptera Simuliidae Polypedilum illinoense grp. 14 Diptera Chironomidae Sphaeriidae 8 Veneroida Polypedilum aviceps 6 Diptera Chironomidae Dubiraphia 5 Coleoptera Elmidae Micromenetus 3 Basommatophora Planorbidae Nais communis 3 Haplotaxida Naididae Physella 3 Basommatophora Physidae Parametriocnemus 3 Diptera Chironomidae Coenagrionidae 2 Odonata Neoporus 2 Coleoptera Dytiscidae Stenelmis 2 Coleoptera Elmidae Caecidotea 2 Isopoda Asellidae Hydrobiidae 1 Mesogastropoda Limnodrilus hoffmeisteri 1 Haplotaxida Tubificidae Dicrotendipes simpsoni 1 Diptera Chironomidae Paratanytarsus sp. c epler 1 Diptera Chironomidae Slavina appendiculata 1 Haplotaxida Naididae Tabanidae 1 Diptera Labrundinia 1 Diptera Chironomidae Nanocladius 1 Diptera Chironomidae 

### Northeast NE09

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Pelecypoda 18 Caecidotea 18 Isopoda Asellidae Ancylidae 17 Basommatophora Micromenetus dilatatus 17 Basommatophora Planorbidae Leptophlebiidae 9 Ephemeroptera Paratanytarsus sp. a epler 9 Diptera Chironomidae Ablabesmyia mallochi 8 Diptera Chironomidae Stenochironomus 3 Diptera Chironomidae Neoporus 3 Coleoptera Dytiscidae Polypedilum illinoense grp. 3 Diptera Chironomidae Neurocordulia alabamensis 2 Odonata Libellulidae Tanytarsus sp. t epler 2 Diptera Chironomidae Labrundinia pilosella 2 Diptera Chironomidae Eclipidrilus 1 Lumbriculida Lumbriculidae Physella 1 Basommatophora Physidae Caenis 1 Ephemeroptera Caenidae Crangonyx 1 Amphipoda Crangonyctidae Notomicrus 1 Coleoptera Noteridae Kiefferulus 1 Diptera Chironomidae Dicrotendipes simpsoni 1 Diptera Chironomidae Tanytarsus sp. m epler 1 Diptera Chironomidae Rheotanytarsus exiguus grp. 1 Diptera Chironomidae Libellula vibrans 1 Odonata Libellulidae Stenelmis 1 Coleoptera Elmidae Oecetis 1 Trichoptera Leptoceridae Clinotanypus 1 Diptera Chironomidae Larsia 1 Diptera Chironomidae Procladius 1 Diptera Chironomidae Parachironomus 1 Diptera Chironomidae 

### Northeast NE10

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Ablabesmyia mallochi 39 Diptera Chironomidae Polypedilum scalaenum grp. 23 Diptera Chironomidae Procladius 15 Diptera Chironomidae Phaenopsectra punctipes grp. 10 Diptera Chironomidae Hemerodromia 9 Diptera Empididae Procambarus 2 Decapoda Cambaridae Polypedilum fallax 2 Diptera Chironomidae Polypedilum halterale grp. 2 Diptera Chironomidae Polypedilum illinoense 2 Diptera Chironomidae Caenis 1 Ephemeroptera Caenidae Orthocladiinae 1 Diptera Parametriocnemus 1 Diptera Chironomidae Cryptochironomus 1 Diptera Chironomidae Dicrotendipes modestus 1 Diptera Chironomidae Elmidae 1 Coleoptera Pisidiidae 1 Veneroida Neoporus 1 Coleoptera Dytiscidae Xestochironomus 1 Diptera Chironomidae Paracladopelma 1 Diptera Chironomidae Chironomus 1 Diptera Chironomidae Neotrichia 1 Trichoptera Hydroptilidae Odonata 1 Odonata Triaenodes 1 Trichoptera Leptoceridae 

### Panhandle

Panh01

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Pelecypoda 19 Tribelos jucundum 19 Diptera Chironomidae Pachydiplax longipennis 9 Odonata Libellulidae Polypedilum tritum 6 Diptera Chironomidae Ceratopogonidae 5 Diptera Zavreliella marmorata 4 Diptera Chironomidae Chironomus 4 Diptera Chironomidae Mideopsis 3 Acariformes Mideopsidae Hyalella azteca 3 Amphipoda Hyalellidae Stenelmis 3 Coleoptera Elmidae Tanytarsus sp. m epler 2 Diptera Chironomidae Nais communis complex 2 Haplotaxida Naididae Nilothauma 2 Diptera Chironomidae Polypedilum illinoense grp. 2 Diptera Chironomidae Dubiraphia vittata 2 Coleoptera Elmidae Slavina appendiculata 2 Haplotaxida Naididae Neoporus 2 Coleoptera Dytiscidae Dicrotendipes simpsoni 1 Diptera Chironomidae Dero digitata complex 1 Haplotaxida Naididae Corethrella 1 Diptera Corethrellidae Cryptochironomus 1 Diptera Chironomidae Macronychus glabratus 1 Coleoptera Elmidae Tanytarsus sp. o epler 1 Diptera Chironomidae Tanytarsus sp. s epler 1 Diptera Chironomidae Parachironomus carinatus 1 Diptera Chironomidae Ancyronyx variegatus 1 Coleoptera Elmidae Micromenetus dilatatus 1 Basommatophora Planorbidae Limnodrilus hoffmeisteri 1 Haplotaxida Tubificidae Thienemannimyia grp. 1 Diptera Chironomidae Kiefferulus 1 Diptera Chironomidae Hydrodroma 1 Acariformes Hydrodromidae Culicidae 1 Diptera 

### Panhandle Panh02

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Rheotanytarsus exiguus grp. 19 Diptera Chironomidae Simulium 15 Diptera Simuliidae Rheocricotopus robacki 15 Diptera Chironomidae Microcylloepus pusillus 6 Coleoptera Elmidae Polypedilum convictum grp. 5 Diptera Chironomidae Eurylophella doris 5 Ephemeroptera Ephemerellidae Polypedilum illinoense grp. 4 Diptera Chironomidae Oecetis 4 Trichoptera Leptoceridae Thienemanniella sp. b epler 3 Diptera Chironomidae Chironomus 3 Diptera Chironomidae Labrundinia pilosella 3 Diptera Chironomidae Baetis 3 Ephemeroptera Baetidae Corynoneura 3 Diptera Chironomidae Polypedilum scalaenum grp. 2 Diptera Chironomidae Labiobaetis 2 Ephemeroptera Baetidae Pentaneura inconspicua 2 Diptera Chironomidae Ablabesmyia mallochi 2 Diptera Chironomidae Pristina synclites 2 Haplotaxida Naididae Caenis 2 Ephemeroptera Caenidae Leptophlebiidae 2 Ephemeroptera Rheotanytarsus distinctissimus grp. 1 Diptera Chironomidae Polypedilum aviceps 1 Diptera Chironomidae Thienemanniella xena 1 Diptera Chironomidae Nilotanypus fimbriatus 1 Diptera Chironomidae Thienemannimyia grp. 1 Diptera Chironomidae Stempellinella 1 Diptera Chironomidae Xestochironomus 1 Diptera Chironomidae Stenochironomus 1 Diptera Chironomidae Nanocladius 1 Diptera Chironomidae Cheumatopsyche 1 Trichoptera Hydropsychidae Stenelmis 1 Coleoptera Elmidae Agnetina 1 Plecoptera Perlidae Argia 1 Odonata Coenagrionidae Lepidoptera 1 Lepidoptera Atractides 1 Acariformes Hygrobatidae Hygrobates 1 Acariformes Hygrobatidae Isonychia 1 Ephemeroptera Oligoneuriidae Stenonema 1 Ephemeroptera Heptageniidae Oribatei 1 Thienemanniella sp. a epler 1 Diptera Chironomidae 

### Panhandle

Panh03

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Chimarra 17 Trichoptera Philopotamidae Polypedilum convictum grp. 14 Diptera Chironomidae Cheumatopsyche 7 Trichoptera Hydropsychidae Calopteryx 6 Odonata Calopterygidae Crangonyx 5 Amphipoda Crangonyctidae Parametriocnemus 4 Diptera Chironomidae Hemerodromia 4 Diptera Empididae Triaenodes 3 Trichoptera Leptoceridae Rheotanytarsus exiguus grp. 3 Diptera Chironomidae Microtendipes pedellus grp. 3 Diptera Chironomidae Gonielmis dietrichi 3 Coleoptera Elmidae Ancyronyx variegatus 3 Coleoptera Elmidae Enallagma 3 Odonata Coenagrionidae Simulium 3 Diptera Simuliidae Eclipidrilus palustris 3 Lumbriculida Lumbriculidae Stempellinella 3 Diptera Chironomidae Hydroptila 2 Trichoptera Hydroptilidae Oecetis 2 Trichoptera Leptoceridae Microtendipes rydalensis grp. 2 Diptera Chironomidae Cambaridae 2 Decapoda Planariidae 2 Tricladida Paranyctiophylax 2 Trichoptera Polycentropodidae Hydropsyche 1 Trichoptera Hydropsychidae Neurocordulia alabamensis 1 Odonata Libellulidae Argia 1 Odonata Coenagrionidae Baetidae 1 Ephemeroptera Lebertia 1 Acariformes Lebertiidae Tanytarsus sp. m epler 1 Diptera Chironomidae Polypedilum illinoense grp. 1 Diptera Chironomidae Polypedilum scalaenum grp. 1 Diptera Chironomidae Tribelos jucundum 1 Diptera Chironomidae Polypedilum aviceps 1 Diptera Chironomidae Pentaneura inconspicua 1 Diptera Chironomidae Arrenurus 1 Acariformes Arrenuridae Meropelopia 1 Diptera Chironomidae Limnodrilus hoffmeisteri 1 Haplotaxida Tubificidae Pseudochironomus 1 Diptera Chironomidae Paraponyx 1 Lepidoptera Pyralidae Stenelmis 1 Coleoptera Elmidae Caecidotea 1 Isopoda Asellidae 

### Panhandle

Panh04

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name N oin d Order Family Comments Polypedilum convictum grp. 30 Diptera Chironomidae Pisidiidae 14 Veneroida Caecidotea 8 Isopoda Asellidae Dubiraphia 7 Coleoptera Elmidae Polypedilum scalaenum grp. 6 Diptera Chironomidae Rheotanytarsus exiguus grp. 6 Diptera Chironomidae Dugesia tigrina 5 Tricladida Dugesiidae Conchapelopia 5 Diptera Chironomidae Hydropsyche 5 Trichoptera Hydropsychidae Oecetis 5 Trichoptera Leptoceridae Ancyronyx variegatus 4 Coleoptera Elmidae Cheumatopsyche 4 Trichoptera Hydropsychidae Tanytarsus sp. l epler 4 Diptera Chironomidae Hyalella azteca 3 Amphipoda Hyalellidae Palpomyia/bezzia grp. 3 Diptera Ceratopogonidae Dicrotendipes modestus 3 Diptera Chironomidae Limnodrilus hoffmeisteri 2 Haplotaxida Tubificidae Corixidae 2 Heteroptera Clinotanypus 2 Diptera Chironomidae Polypedilum illinoense grp. 2 Diptera Chironomidae Thienemannimyia grp. 1 Diptera Chironomidae Pristinella longisoma 1 Haplotaxida Naididae Microcylloepus pusillus 1 Coleoptera Elmidae Tanytarsus sp. m epler 1 Diptera Chironomidae Thienemanniella sp. a epler 1 Diptera Chironomidae Stenelmis 1 Coleoptera Elmidae Helodidae 1 Coleoptera Libellulidae 1 Odonata Chironomus 1 Diptera Chironomidae Tvetenia discoloripes grp. 1 Diptera Chironomidae Tanytarsus sp. e epler 1 Diptera Chironomidae Rheotanytarsus distinctissimus grp. 1 Diptera Chironomidae Labrundinia neopilosella 1 Diptera Chironomidae Larsia decolorata 1 Diptera Chironomidae 

### Panhandle

Panh05

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Tribelos j ucundum 26 Diptera Chironomidae Dubira p hia vittata 7 Coleoptera Elmidae Eur y lo p hella doris 7 Ephemeroptera Ephemerellidae Oecetis g eor g ia 4 Trichoptera Leptoceridae Pol yp edilum illinoense g r p . 4 Diptera Chironomidae Le p to p hlebiidae 3 Ephemeroptera Cr yp tochironomus 3 Diptera Chironomidae Thienemannim y ia g r p . 3 Diptera Chironomidae Probezzia 3 Diptera Ceratopogonidae Triaenodes 2 Trichoptera Leptoceridae Ench y traeidae 2 Haplotaxida Palaemonetes kadiakensis 2 Decapoda Palaemonidae Pol yp edilum convictum g r p . 2 Diptera Chironomidae Ablabesm y ia mallochi 2 Diptera Chironomidae Tan y tarsus s p . d e p ler 2 Diptera Chironomidae Tan y tarsus s p . o e p ler 2 Diptera Chironomidae Cerato p o g on 2 Diptera Ceratopogonidae Stenacron floridense 2 Ephemeroptera Heptageniidae Microc y lloe p us p usillus 2 Coleoptera Elmidae Concha p elo p ia 2 Diptera Chironomidae Aeshnidae 2 Odonata S p haeriidae ( mollusca ) 2 Veneroida Neurecli p sis 1 Trichoptera Polycentropodidae Stem p ellinella 1 Diptera Chironomidae Pol yp edilum scalaenum g r p . 1 Diptera Chironomidae Stelechom y ia p er p ulchra 1 Diptera Chironomidae Paraclado p elma undine 1 Diptera Chironomidae Rheocricoto p us robacki 1 Diptera Chironomidae Gonielmis dietrichi 1 Coleoptera Elmidae Anc y ron y x varie g atus 1 Coleoptera Elmidae Stenochironomus 1 Diptera Chironomidae Sciaridae 1 Diptera Atractides 1 Acariformes Hygrobatidae Caecidotea 1 Isopoda Asellidae Enalla g ma 1 Odonata Coenagrionidae Ecto p ria 1 Coleoptera Psephenidae Stenelmis 1 Coleo p tera Elmidae Panhandle

Panh06

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Ecli p idrilus p alustris 31 Lumbriculida Lumbriculidae Tan y tarsus s p . t e p ler 19 Diptera Chironomidae Tan y tarsus s p . c e p ler 13 Diptera Chironomidae Concha p elo p ia 10 Diptera Chironomidae Cr yp tochironomus 8 Diptera Chironomidae Tan y tarsus s p . u e p ler 5 Diptera Chironomidae Stenelmis 5 Coleoptera Elmidae Cricoto p us bicinctus 5 Diptera Chironomidae Pol yp edilum scalaenum g r p . 4 Diptera Chironomidae Saetheria 4 Diptera Chironomidae Pol yp edilum tritum 3 Diptera Chironomidae Pol yp edilum illinoense 2 Diptera Chironomidae Cladotan y tarsus 2 Diptera Chironomidae Ablabesm y ia mallochi 2 Diptera Chironomidae Ench y traeidae 1 Haplotaxida Ph y sella 1 Basommatophora Physidae Bratislavia unidentata 1 Haplotaxida Naididae Baetis frondalis 1 Ephemeroptera Baetidae Cheumato p s y che 1 Trichoptera Hydropsychidae Gutti p elo p ia g utti p ennis 1 Diptera Chironomidae Celina 1 Coleoptera Dytiscidae H y daticus 1 Coleo p tera D y tiscidae Panhandle

Panh07

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Stenelmis 18 Coleoptera Elmidae Caenis 13 Ephemeroptera Caenidae Oecetis 12 Trichoptera Leptoceridae Tribelos fuscicornis 11 Diptera Chironomidae Polypedilum convictum grp. 6 Diptera Chironomidae Dubiraphia 5 Coleoptera Elmidae Cheumatopsyche 5 Trichoptera Hydropsychidae Macromia taeniolata 4 Odonata Libellulidae Limnodrilus hoffmeisteri 4 Haplotaxida Tubificidae Cryptochironomus 4 Diptera Chironomidae Argia 3 Odonata Coenagrionidae Ablabesmyia rhamphe grp. 2 Diptera Chironomidae Acerpenna pygmaea 2 Ephemeroptera Baetidae Hyalella azteca 2 Amphipoda Hyalellidae Hemerodromia 2 Diptera Empididae Neotrichia 2 Trichoptera Hydroptilidae Cernotina 2 Trichoptera Polycentropodidae Ancylidae 2 Basommatophora Enchytraeidae 2 Haplotaxida Tricorythodes albilineatus 1 Ephemeroptera Leptohyphidae Polypedilum scalaenum grp. 1 Diptera Chironomidae Ablabesmyia (karelia) grp. 1 Diptera Chironomidae Planorbidae 1 Basommatophora Heptageniidae 1 Ephemeroptera Enallagma 1 Odonata Coenagrionidae Triaenodes 1 Trichoptera Leptoceridae Dineutus 1 Coleoptera Gyrinidae Gammarus 1 Amphipoda Gammaridae Pisidiidae 1 Veneroida Hydrobiidae 1 Mesogastropoda Nyctiophylax 1 Trichoptera Polycentropodidae Stenochironomus 1 Diptera Chironomidae Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Polypedilum illinoense grp. 1 Diptera Chironomidae 

### Peninsula Pen08

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Cricotopus bicinctus 63 Diptera Chironomidae Limnodrilus hoffmeisteri 22 Haplotaxida Tubificidae Lumbriculus variegatus 19 Lumbriculida Lumbriculidae Cricotopus or orthocladius 15 Diptera Chironomidae Bratislavia unidentata 13 Haplotaxida Naididae Physella 9 Basommatophora Physidae Nais pardalis 8 Haplotaxida Naididae Enchytraeidae 2 Haplotaxida Dero nivea 2 Haplotaxida Naididae Culicidae 1 Diptera Chironomus 1 Diptera Chironomidae Dero trifida 1 Haplotaxida Naididae Pristinella jenkinae 1 Haplotaxida Naididae Argia fumipennis 1 Odonata Coenagrionidae Dero vaga 1 Haplotaxida Naididae Mooreobdella 1 Arhynchobdellida Erpobdellidae 

### Panhandle

Panh09

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Limnodrilus hoffmeisteri 86 Haplotaxida Tubificidae Chironomus 80 Diptera Chironomidae 

### Panhandle

Panh10

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Limnodrilus hoffmeisteri 27 Haplotaxida Tubificidae Goeldichironomus holoprasinus 15 Diptera Chironomidae Aulodrilus pigueti 13 Haplotaxida Tubificidae Physella 11 Basommatophora Physidae Micromenetus dilatatus 11 Basommatophora Planorbidae Sphaeriidae 6 Veneroida Polypedilum illinoense grp. 6 Diptera Chironomidae Dero vaga 5 Haplotaxida Naididae Dero digitata complex 5 Haplotaxida Naididae Pachydiplax longipennis 4 Odonata Libellulidae Cladopelma 3 Diptera Chironomidae Dicrotendipes modestus 3 Diptera Chironomidae Ancylidae 2 Basommatophora Hydrocanthus 2 Coleoptera Noteridae Helobdella triserialis 2 Rhynchobdellida Glossiphoniidae Coenagrionidae 1 Odonata Gloiobdella elongata 1 Rhynchobdellida Glossiphoniidae Pomacea paludosa 1 Mesogastropoda Ampullariidae Haber speciosus 1 Haplotaxida Tubificidae Planorbella 1 Basommatophora Planorbidae 

### Peninsula Pen01

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Pyrogophorus platyrachis 34 Mesogastropoda Hydrobiidae Cheumatopsyche 33 Trichoptera Hydropsychidae Corbicula fluminea 9 Veneroida Corbiculidae Stenelmis hungerfordi 6 Coleoptera Elmidae Microcylloepus pusillus 6 Coleoptera Elmidae Caenis diminuta 6 Ephemeroptera Caenidae Goeldichironomus holoprasinus 5 Diptera Chironomidae Macromia taeniolata 4 Odonata Libellulidae Hyalella azteca 3 Amphipoda Hyalellidae Pentaneura inconspicua 3 Diptera Chironomidae Pyralidae 2 Lepidoptera Ablabesmyia rhamphe grp. 2 Diptera Chironomidae Polypedilum convictum grp. 2 Diptera Chironomidae Polypedilum halterale grp. 2 Diptera Chironomidae Helobdella triserialis 2 Rhynchobdellida Glossiphoniidae Ischnura 1 Odonata Coenagrionidae Polypedilum scalaenum grp. 1 Diptera Chironomidae Polypedilum trigonum 1 Diptera Chironomidae Hydrocanthus regius 1 Coleoptera Noteridae Palaemonetes paludosus 1 Decapoda Palaemonidae Ancylidae 1 Basommatophora Stenochironomus 1 Diptera Chironomidae Pelocoris 1 Heteroptera Naucoridae Mesovelia 1 Heteroptera Mesoveliidae Dubiraphia 1 Coleoptera Elmidae 

### Peninsula Pen02

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Pelecypoda 30 Dubiraphia vittata 29 Coleoptera Elmidae Hydrobiidae 10 Mesogastropoda Stenelmis 10 Coleoptera Elmidae Pachydiplax longipennis 5 Odonata Libellulidae Caecidotea 4 Isopoda Asellidae Arrenurus 3 Acariformes Arrenuridae Chironomus 3 Diptera Chironomidae Caenis 2 Ephemeroptera Caenidae Goeldichironomus holoprasinus 2 Diptera Chironomidae Scirtes 2 Coleoptera Scirtidae Kiefferulus 2 Diptera Chironomidae Hyalella azteca 2 Amphipoda Hyalellidae Dicrotendipes simpsoni 2 Diptera Chironomidae Polypedilum halterale grp. 2 Diptera Chironomidae Oniscidae 1 Isopoda Microcylloepus pusillus 1 Coleoptera Elmidae 

### Peninsula Pen03

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Polypedilum illinoense grp. 46 Diptera Chironomidae Asheum beckae 12 Diptera Chironomidae Goeldichironomus natans 7 Diptera Chironomidae Melanoides 7 Mesogastropoda Thiaridae Tanytarsus sp. t epler 6 Diptera Chironomidae Argia 6 Odonata Coenagrionidae Hyalella azteca 4 Amphipoda Hyalellidae Limnodrilus hoffmeisteri 3 Haplotaxida Tubificidae Orthemis ferruginea 2 Odonata Libellulidae Ceratopogonidae 2 Diptera Cambaridae 2 Decapoda Enchytraeidae 2 Haplotaxida Hirudinea 2 Polypedilum tritum 1 Diptera Chironomidae Hydrobiidae 1 Mesogastropoda Physella 1 Basommatophora Physidae Chironomus 1 Diptera Chironomidae Larsia 1 Diptera Chironomidae Microvelia 1 Heteroptera Veliidae Planorbidae 1 Basommatophora 

### Peninsula Pen04

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Hyalella azteca 53 Amphipoda Hyalellidae Amnicola dalli 10 Mesogastropoda Bithyniidae Baetis propinquus 9 Ephemeroptera Baetidae Cricotopus or orthocladius 8 Diptera Chironomidae Oligochaeta 6 Cricotopus bicinctus 4 Diptera Chironomidae Microcylloepus pusillus 3 Coleoptera Elmidae Hydroptila 3 Trichoptera Hydroptilidae Polypedilum illinoense grp. 2 Diptera Chironomidae Elimia floridensis 2 Mesogastropoda Pleuroceridae Planorbella duryi 2 Basommatophora Planorbidae Tanytarsus sp. e epler 2 Diptera Chironomidae Rheotanytarsus exiguus grp. 2 Diptera Chironomidae Cheumatopsyche 2 Trichoptera Hydropsychidae Tricorythodes albilineatus 2 Ephemeroptera Leptohyphidae Oecetis 1 Trichoptera Leptoceridae Rheotanytarsus distinctissimus grp. 1 Diptera Chironomidae Polypedilum convictum grp. 1 Diptera Chironomidae Astacidae 1 Decapoda Turbellaria 1 Hydropsyche 1 Trichoptera Hydropsychidae Stenelmis 1 Coleoptera Elmidae Pentaneura inconspicua 1 Diptera Chironomidae Palaemonetes paludosus 1 Decapoda Palaemonidae Rhagovelia choreutes 1 Heteroptera Veliidae Stenochironomus 1 Diptera Chironomidae Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Hemerodromia 1 Diptera Empididae Baetis intercalaris 1 Ephemeroptera Baetidae Simulium 1 Diptera Simuliidae 

### Peninsula Pen05

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Cheumatopsyche 20 Trichoptera Hydropsychidae Microcylloepus pusillus 15 Coleoptera Elmidae Sphaeriidae(mollusca) 10 Veneroida Corbicula fluminea 9 Veneroida Corbiculidae Ablabesmyia rhamphe grp. 6 Diptera Chironomidae Polypedilum scalaenum grp. 4 Diptera Chironomidae Hyalella azteca 4 Amphipoda Hyalellidae Tubificidae 4 Haplotaxida Dubiraphia 3 Coleoptera Elmidae Polypedilum illinoense grp. 2 Diptera Chironomidae Hydrobiidae 2 Mesogastropoda Cryptochironomus 2 Diptera Chironomidae Polypedilum tritum 2 Diptera Chironomidae Ablabesmyia peleensis 2 Diptera Chironomidae Ablabesmyia mallochi 2 Diptera Chironomidae Stenelmis 2 Coleoptera Elmidae Gloiobdella elongata 2 Rhynchobdellida Glossiphoniidae Polypedilum halterale grp. 1 Diptera Chironomidae Laevapex fuscus 1 Basommatophora Ancylidae Hebetancylus excentricus 1 Basommatophora Ancylidae Argia sedula 1 Odonata Coenagrionidae Micromenetus dilatatus 1 Basommatophora Planorbidae Laevapex peninsulae 1 Basommatophora Ancylidae Clinotanypus 1 Diptera Chironomidae Astacidae 1 Decapoda Dineutus 1 Coleoptera Gyrinidae 

### Peninsula Pen06

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Hyalella azteca 21 Amphipoda Hyalellidae Caenis 15 Ephemeroptera Caenidae Neotrichia 10 Trichoptera Hydroptilidae Pyrogophorus platyrachis 8 Mesogastropoda Hydrobiidae Limnodrilus hoffmeisteri 7 Haplotaxida Tubificidae Tribelos fuscicornis 7 Diptera Chironomidae Pisidiidae 5 Veneroida Ablabesmyia mallochi 3 Diptera Chironomidae Tanytarsus sp. g epler 2 Diptera Chironomidae Polypedilum scalaenum grp. 2 Diptera Chironomidae Polypedilum halterale grp. 2 Diptera Chironomidae Rheotanytarsus distinctissimus grp. 2 Diptera Chironomidae Stenochironomus 2 Diptera Chironomidae Larsia decolorata 2 Diptera Chironomidae Tanytarsus sp. d epler 2 Diptera Chironomidae Tanytarsus sp. e epler 2 Diptera Chironomidae Tanytarsus sp. t epler 2 Diptera Chironomidae Ablabesmyia (karelia) grp. 1 Diptera Chironomidae Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Cernotina 1 Trichoptera Polycentropodidae Clinotanypus 1 Diptera Chironomidae Oecetis 1 Trichoptera Leptoceridae Micromenetus 1 Basommatophora Planorbidae Cladotanytarsus 1 Diptera Chironomidae Cryptochironomus 1 Diptera Chironomidae Polypedilum illinoense grp. 1 Diptera Chironomidae Ablabesmyia rhamphe grp. 1 Diptera Chironomidae Nanocladius 1 Diptera Chironomidae Microcylloepus pusillus 1 Coleoptera Elmidae 

### Peninsula Pen07

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Stenelmis 18 Coleoptera Elmidae Caenis 13 Ephemeroptera Caenidae Oecetis 12 Trichoptera Leptoceridae Tribelos fuscicornis 11 Diptera Chironomidae Polypedilum convictum grp. 6 Diptera Chironomidae Dubiraphia 5 Coleoptera Elmidae Cheumatopsyche 5 Trichoptera Hydropsychidae Macromia taeniolata 4 Odonata Libellulidae Limnodrilus hoffmeisteri 4 Haplotaxida Tubificidae Cryptochironomus 4 Diptera Chironomidae Argia 3 Odonata Coenagrionidae Ablabesmyia rhamphe grp. 2 Diptera Chironomidae Acerpenna pygmaea 2 Ephemeroptera Baetidae Hyalella azteca 2 Amphipoda Hyalellidae Hemerodromia 2 Diptera Empididae Neotrichia 2 Trichoptera Hydroptilidae Cernotina 2 Trichoptera Polycentropodidae Ancylidae 2 Basommatophora Enchytraeidae 2 Haplotaxida Tricorythodes albilineatus 1 Ephemeroptera Leptohyphidae Polypedilum scalaenum grp. 1 Diptera Chironomidae Ablabesmyia (karelia) grp. 1 Diptera Chironomidae Planorbidae 1 Basommatophora Heptageniidae 1 Ephemeroptera Enallagma 1 Odonata Coenagrionidae Triaenodes 1 Trichoptera Leptoceridae Dineutus 1 Coleoptera Gyrinidae Gammarus 1 Amphipoda Gammaridae Pisidiidae 1 Veneroida Hydrobiidae 1 Mesogastropoda Nyctiophylax 1 Trichoptera Polycentropodidae Stenochironomus 1 Diptera Chironomidae Palpomyia/bezzia grp. 1 Diptera Ceratopogonidae Polypedilum illinoense grp. 1 Diptera Chironomidae 

### Peninsula Pen08

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Dero pectinata 61 Haplotaxida Naididae Einfeldia sp. a epler 23 Diptera Chironomidae Nais communis 8 Haplotaxida Naididae Pristina aequiseta 7 Haplotaxida Naididae Hyalella azteca 7 Amphipoda Hyalellidae Pristina leidyi 4 Haplotaxida Naididae Tanypus carinatus 4 Diptera Chironomidae Larsia decolorata 4 Diptera Chironomidae Enallagma 3 Odonata Coenagrionidae Chironomus 3 Diptera Chironomidae Dasyhelea 2 Diptera Ceratopogonidae Haemonais waldvogeli 2 Haplotaxida Naididae Caenis 2 Ephemeroptera Caenidae Arrenurus 2 Acariformes Arrenuridae Suphisellus insularis 2 Coleoptera Noteridae Palaemonetes paludosus 2 Decapoda Palaemonidae Bratislavia unidentata 2 Haplotaxida Naididae Allonais paraguayensis 2 Haplotaxida Naididae Slavina appendiculata 2 Haplotaxida Naididae Celina 1 Coleoptera Dytiscidae Parakiefferiella sp. c epler 1 Diptera Chironomidae Planorbidae 1 Basommatophora Unionicola 1 Acariformes Unionicolidae Limnesia 1 Acariformes Limnesiidae Physella 1 Basommatophora Physidae Turbellaria 1 Tanytarsus sp. c epler 1 Diptera Chironomidae Polypedilum trigonum 1 Diptera Chironomidae Mesonoterus 1 Coleoptera Noteridae Pseudochironomus 1 Diptera Chironomidae Notomicrus nanulus 1 Coleoptera Noteridae Limnodrilus hoffmeisteri 1 Haplotaxida Tubificidae 

### Peninsula Pen09

### Expert: Bioregion: BCGSampleID: Initial Score: Final Score: Scientific Name Noind Order Family Comments Goeldichironomus holoprasinus 92 Diptera Chironomidae Anax junius 6 Odonata Aeshnidae Ischnura 4 Odonata Coenagrionidae Tabanidae 2 Diptera Odontomyia 2 Diptera Stratiomyidae Paramerina 1 Diptera Chironomidae Libellula semifasciata 1 Odonata Libellulidae Suphis inflatus 1 Coleoptera Noteridae Culicidae 1 Diptera Tanypus carinatus 1 Diptera Chironomidae 

### Peninsula Pen10Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A8 -1 

## Appendix A 8 . Memo to FDEP Regarding Interval and Equivalence Tests for the SCI

Cross

- references:
Appendix B-14: Memo to FDEP Regarding Interval and Equivalence Tests for the SCI . U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule . January 14, 2010. EPA- HQ

- OW -2009- 0596, FRL-9105-1.
Appendix 3-C . U.S.EPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters. United States Environmental Protection Agency, Office of Water, Office of Science and Technology, Health and Ecological Criteria Division, Washington, DC.

# Memo to FDEP Regarding Interval and Equivalence Tests for the SCI

(Appendix 3-C from USEPA. 2009. Proposed Methods and Approaches for Developing Numeric Nutrient Criteria for Florida’s Inland Waters . United States Environm ental Protection Agency, Office of Water, Office of Science and Technol ogy, Health and Ecological Criteria Division, Washington, DC.)

## Appendix 3-C



# Memo from Leska Fo re regarding interva l and equivalence tests for the Strea m Condition Index

### 2 Statistical Design 136 NW 40th St. Seattle, WA 98107 (206) 708-5048



### DATE: April 9, 2009

TO: Russ Frydenborg, Florida DEP

FROM: Leska Fore, Statistical Design

RE: Statistical Framework for Defining Impairment for SCI



# Objective

Identify a statistical test and approach fo r defining impairment status for the case in which a single test site is compared to a distribution of reference sites. Florida DEP’s stream condition index (SCI) for macroinvertebrate stream samples is used as a case study.



# Why use a statistical framework?

 A statistical model provides a hypothesis and a decision rule for determining whether a test site differs from reference condition.

 Uncertainty associated with an impa irment decision can be estimated.

 Type I (true reference site called impair ed) and Type II (true impaired site called reference) error can be compared and ba lanced to be equally protective of the resource and the regulated community.

 Provides a framework for future, unforeseen questions and needs, for example, how to deal with two replicat e visits to a test site.

 Methods are symmetric in that they can be equally applied to de-listing or listing a site as impaired.

 Makes listing decisions more robust to legal challenge.



# Statistical decision ≠ Stakeholder decision

Defining a threshold for biologi cal impairment involves scientif ic information and social values. Statistics can support this decision, but cannot ultimately be used to make the 

# MEMORANDUM

### 3 decision. Once a threshold has been defined, a statistical framework can be used to structure the decision process ar ound the line of impairment.

This document addresses the statistical uncertainty around the threshold line.



# Criteria for selection of a statistical framework

 Applicable to our situation: comparison of one test site to a reference population.

 Legally and statistically defensible.

 Provides estimate of uncertainty asso ciated with impairment status calls.

 Can estimate false positive and negative rates.

 Can be used to model the effect of repeat visits to a site.



# Candidate statistical tests

Much of the literature related to thres hold testing for environmental compliance addresses the comparison of water chemistr y samples to a threshold (SWRCB, 2004). This testing scenario for chemical contam inants is somewhat the opposite from our situation in that contaminant thresholds are defined as a single value with no associated uncertainty, that is, no set of values from reference sites. Fo r biocriteria, thresholds are derived from a population of reference sites, not a single value. Furthermore, water chemistry test sites have multiple samples ra ther than a single value as is typical for biocriteria.
For water chemistry, the variability is associ ated with the test s ite rather than the reference sites. In contrast, for biocriteria we are comparing one test site value to multiple reference site values. Expectations for bioc riteria are based on local reference condition while thresholds for toxics and contaminants tend to be expressed as a single threshold that is nationally applied. Most of the papers related to statistical testing for thresholds address the chemical contaminant model.
An alternative approach for biocriteria thre shold testing was descri bed by Kilgour et al. (1998). Their method was based on interval and e quivalence tests. Both tests can be used to evaluate whether an observation at a test location falls within or outside of the normal range of variation for reference locations. Wh en results from the tests are interpreted together, errors due to false positives and negatives are equally likely.

### 4 Table 1. Candidate statistical tests for comparing SC I values at a test site to a threshold derived from reference sites.

Test name Application to biocriteria threshold

Recommended : Combination of interval and equivalence tests (Kilgour et al., 1998) Both tests are designed to compare 1 test site against a population of reference sites; pair of tests controls for both Type I and Type II error

Two-sample t test Assumes multiple samples from both the test population and the reference population

One-sample t test Comparison to the mean, not to percentiles of the distribution; low power to detect differences

One-sample Wilcoxon signed rank test Nonparam etric analog to one-sample t test, compares median of a test population to a threshold; not designed to compare percentiles

Confidence interval around a proportion (Gibbons, 2001) Designed to test what proportion of test samples exceed a threshold; multiple test samples, one reference threshold

Exact binomial test Widely recommended for chemical testing of % exceedances of a threshold; compares multiple test values to a single reference value



# Interval and Equivalence Testing

The specific statistical methods described by K ilgour et al. (1998) are not particularly novel, i.e., the comparison of a statistic to th e noncentral F distribu tion, but the pairing of two tests (interval and equiva lence tests) and the applica tion to bioassessment endpoints represents a new approach. Their approach wa s developed specifically for the comparison of a single test site to a populat ion of reference sites for meas ures of biological condition.
Both the interval and the equivalence tests can be applied to the comparison of a single test site value with values from a population of reference sites. Both tests have a low Type I error rate (false positive), but they can be used to balance false positives and negatives because they test the opposite null hypotheses. The in terval null hypothesis tests for difference from reference and the equiva lence null hypothe sis tests for equality to reference (Table 2). In this way, the tests are symmetric. By pairing these two tests, we can balance for the error rates associated with falsely determining a reference site to be impaired (Type I) and missing a site that is truly impaired (Type II; Appendix A).
Although not relevant for this application to the SCI, the approach can also be generalized to a multivariate comparison of multiple indicators (e.g., metrics) against their respective th resholds (Bowman and Somers, 2006).

### 5 Table 2. Comparison of the interval and equivale nce tests, hypotheses tested by each and their advantages and disadvantages relative to decisions about impairment.

Statistical test H O H A Advantages Disadvantages

Interval test Test site is within the range of reference sites Test site is not within the range of reference sites Protects the polluter (guards against unnecessary listing) Not as sensitive at detecting impairment

Equivalence test Test site is not within the range of reference sites Test site is within the range of reference sites Protects the environment (very sensitive to impairment) Very sensitive and may identify reference sites as impaired



# How do we specify the test?

Both tests use the same test statistic, the fa miliar F-statistic. Because we are testing for differences not from the mean, but for so me distance from the mean, e.g., the 10 th 

### percentile (our defined threshold), we need to use a noncentral F test. The null and alternate hypotheses are mirror images of each for the interval a nd equivalence tests.

Interval test

The null hypothesis is that the test and reference sites are the same . The alternative hypothesis is that test an d reference sites are the different

.
H O 

### (null): 

#      T ref 

### , or “The difference between the mean SCI of the reference sites
and the SCI at the test site is less than delta.” Delta can be any specified value, e.g., the 10 th 

### percentile of SCI values for reference sites.

H A 

### (alternate): 

#      T ref 

### , or “The test site is more different than the specified difference (delta) from the mean SCI.”

Equivalence test

The null hypothesis is that the te st and reference sites are different . The alternative hypothesis is that test an d reference sites are the same

.
H O 

### : 

#      T ref 

### , or “The difference between the m ean SCI of the reference sites and
the SCI at the test site is more than delta.”

### 6 H A 

### : 

#      T ref 

### , or “The test site is not different from the specified difference from the
mean SCI for reference sites.”

Test statistic 

#  

2 2



### 1         



ref ref

ref



### n s TX F



# How does statistical test work?

Suppose we go to a stream site and sample fo r SCI and calculate a value of 46 out of a possible range of values from 0–100. We want to compare this observed value to the 10 th 

### percentile of SCI values for re ference sites (Table 3). The 10 th 

### percentile is derived from an assumption that reference sites follow an approximately normal distribution. The table value for the 10 th 

### percentile of a normal distribut ion (–1.28) is used to define 10 th 

### percentile of the reference dist ribution. This z-table value is also used to define the noncentrality parameter, lambda.

We calculate the F-statistic and use a statis tical program (e.g., R or Excel) to look up the p-value associated with the F-statistic with 1 and 63 degr ees of freedom and determine that we can reject the interval test and conc lude that the test s ite is different from reference condition.

Table 3. Worked example for comparing SCI at a te st site to SCI values at reference sites.

Description Value

Mean SCI (reference sites) 66.3

Standard deviation of SCI (reference sites) 12.6

Number of reference sites 64

10 th Percentile of SCI (reference sites) 66.3–(1.28*12.6) = 50

SCI for test site 46

Standardized z-value for 10 th percentile of reference (one-tailed test); used to define difference (delta) we are testing for –1.28

### 7 F-statistic 

#  

2 2



### 64 1 6.12 463.66

         = 166.1

Degrees of freedom (1, 63)

Lambda, noncentrality parameter; this value represents how far from the mean the test site should be, i.e., lower than the 10 th percentile 64 * (1.28) 2 = 104.9

p-value for F-statistic (interval test) p = 0.04, significantly lower than the 10 th %tile of reference

Conclusion “We are 95% confident the test site is impaired with impairment defined as below the 10 th percentile of reference.”



# Statistical Decision Rules for SCI

We can use the statistical framework of in terval and equivalence testing to estimate uncertainty for SCI values at a test site ba sed on different definitions of impairment.

Table 4. Statistical decision rules for SCI values at a test site for threshold values defined as the 1 st , 5 th , and 10 th percentiles of SCI values for reference sites. Shown are values for p < 0.05. N = 1 SCI value for each site.

Impairment threshold (description) Impairment threshold (numeric) Impaired Undetermined Reference

2.5 th percentile of reference 42 <37 37–45 >45

5 th percentile of reference 46 <41 41–49 >49

10 th percentile of reference 50 <47 47–53 >53



# Summary

 Caveat: Statistical tests provide a framework for comparison and testing around a threshold, but cannot be used alone to defi ne impairment thresholds. That decision also relies on scientific unde rstanding and social values.

The method described here combines two stat istical tests, the interval test and the equivalence test, to compare a si ngle test site value to a set of values from a population of reference sites. This approach satisfies most of our criteria for a statistical framework for

### 8 a biocriteria threshold. Pairing the interval a nd equivalence tests to evaluate percentiles from the reference site distribution represents a simple statistical decision rule that is easy to describe and legally defensible. We can estimate error rates for false positives and false negatives and balance them. The method is symmetric and can be easily inverted for delisting without any change to Type I or Type II errors.
This approach does not deal exp licitly with the case in which more than one sample value is collected from a test site. This problem c ould be addressed in two ways. For the simple case of two repeat visits to a test site, a new distribution of reference sites could be developed based on the average SCI value for tw o repeat visits to each reference site. These could be the most recent visit or two visits that were randomly chosen.
A more general approach to incorporating test site variability could be accomplished with a simulation. Using our estimate of within -site variability, a di stribution around point estimates of SCI could be defined. Repeat sa mpling from this distribution could be used to generate a distribution of F-statistics. In this way, we could model the variability associated with a single SCI for a test site.
Combining the interval and equivalence tests provides a statistical framework to support impairment decisions, and is particularly suit ed to biocriteria becau se a single test value is compared to a population of reference values.


# Appendix A

Example showing application of interval and equivalence testing for determining the status of a test site. The threshold for impairment is defined as the 10 th percentile of SCI values for reference sites. Shown are the SCI value, difference between mean of reference and SCI, numerator and denominator for the F statistic, F, p-value for F fo r the interval and equivalence tests, status call, and result for the interval and equivalence tests. See Table 3 for values used in calculations. SCI values greater than or equal to the mean for reference sites (SCI = 66–100) are not shown.
SCI Mu-imp F_Num F_Den F P_Int P_Equ STATUS Interv. Equiv. 1 65.3 4264.1 2.48 1719.0 0.00 1.00 IMPAIRED >delta >=delta 2 64.3 4134.5 2.48 1666.7 0.00 1.00 IMPAIRED >delta >=delta 3 63.3 4006.9 2.48 1615.3 0.00 1.00 IMPAIRED >delta >=delta 4 62.3 3881.3 2.48 1564.6 0.00 1.00 IMPAIRED >delta >=delta 5 61.3 3757.7 2.48 1514.8 0.00 1.00 IMPAIRED >delta >=delta 6 60.3 3636.1 2.48 1465.8 0.00 1.00 IMPAIRED >delta >=delta 7 59.3 3516.5 2.48 1417.6 0.00 1.00 IMPAIRED >delta >=delta 8 58.3 3398.9 2.48 1370.2 0.00 1.00 IMPAIRED >delta >=delta 9 57.3 3283.3 2.48 1323.6 0.00 1.00 IMPAIRED >delta >=delta 10 56.3 3169.7 2.48 1277.8 0.00 1.00 IMPAIRED >delta >=delta 11 55.3 3058.1 2.48 1232.8 0.00 1.00 IMPAIRED >delta >=delta 12 54.3 2948.5 2.48 1188.6 0.00 1.00 IMPAIRED >delta >=delta 13 53.3 2840.9 2.48 1145.2 0.00 1.00 IMPAIRED >delta >=delta 14 52.3 2735.3 2.48 1102.7 0.00 1.00 IMPAIRED >delta >=delta 15 51.3 2631.7 2.48 1060.9 0.00 1.00 IMPAIRED >delta >=delta 16 50.3 2530.1 2.48 1019.9 0.00 1.00 IMPAIRED >delta >=delta 17 49.3 2430.5 2.48 979.8 0.00 1.00 IMPAIRED >delta >=delta

### 9 SCI Mu-imp F_Num F_Den F P_Int P_Equ STATUS Interv. Equiv.

18 48.3 2332.9 2.48 940.4 0.00 1.00 IMPAIRED >delta >=delta 19 47.3 2237.3 2.48 901.9 0.00 1.00 IMPAIRED >delta >=delta 20 46.3 2143.7 2.48 864.2 0.00 1.00 IMPAIRED >delta >=delta 21 45.3 2052.1 2.48 827.2 0.00 1.00 IMPAIRED >delta >=delta 22 44.3 1962.5 2.48 791.1 0.00 1.00 IMPAIRED >delta >=delta 23 43.3 1874.9 2.48 755.8 0.00 1.00 IMPAIRED >delta >=delta 24 42.3 1789.3 2.48 721.3 0.00 1.00 IMPAIRED >delta >=delta 25 41.3 1705.7 2.48 687.6 0.00 1.00 IMPAIRED >delta >=delta 26 40.3 1624.1 2.48 654.7 0.00 1.00 IMPAIRED >delta >=delta 27 39.3 1544.5 2.48 622.6 0.00 1.00 IMPAIRED >delta >=delta 28 38.3 1466.9 2.48 591.3 0.00 1.00 IMPAIRED >delta >=delta 29 37.3 1391.3 2.48 560.9 0.00 1.00 IMPAIRED >delta >=delta 30 36.3 1317.7 2.48 531.2 0.00 1.00 IMPAIRED >delta >=delta 31 35.3 1246.1 2.48 502.3 0.00 1.00 IMPAIRED >delta >=delta 32 34.3 1176.5 2.48 474.3 0.00 1.00 IMPAIRED >delta >=delta 33 33.3 1108.9 2.48 447.0 0.00 1.00 IMPAIRED >delta >=delta 34 32.3 1043.3 2.48 420.6 0.00 1.00 IMPAIRED >delta >=delta 35 31.3 979.7 2.48 394.9 0.00 1.00 IMPAIRED >delta >=delta 36 30.3 918.1 2.48 370.1 0.00 1.00 IMPAIRED >delta >=delta 37 29.3 858.5 2.48 346.1 0.00 1.00 IMPAIRED >delta >=delta 38 28.3 800.9 2.48 322.9 0.00 1.00 IMPAIRED >delta >=delta 39 27.3 745.3 2.48 300.4 0.00 1.00 IMPAIRED >delta >=delta 40 26.3 691.7 2.48 278.8 0.00 1.00 IMPAIRED >delta >=delta 41 25.3 640.1 2.48 258.0 0.00 1.00 IMPAIRED >delta >=delta 42 24.3 590.5 2.48 238.0 0.00 1.00 IMPAIRED >delta >=delta 43 23.3 542.9 2.48 218.9 0.00 1.00 IMPAIRED >delta >=delta 44 22.3 497.3 2.48 200.5 0.01 0.99 IMPAIRED >delta >=delta 45 21.3 453.7 2.48 182.9 0.02 0.98 IMPAIRED >delta >=delta 46 20.3 412.1 2.48 166.1 0.04 0.96 IMPAIRED >delta >=delta 47 19.3 372.5 2.48 150.2 0.09 0.91 Undetermined <=delta >=delta 48 18.3 334.9 2.48 135.0 0.18 0.82 Undetermined <=delta >=delta 49 17.3 299.3 2.48 120.7 0.31 0.69 Undetermined <=delta >=delta 50 16.3 265.7 2.48 107.1 0.48 0.52 Undetermined <=delta >=delta 51 15.3 234.1 2.48 94.4 0.67 0.33 Undetermined <=delta >=delta 52 14.3 204.5 2.48 82.4 0.82 0.18 Undetermined <=delta >=delta 53 13.3 176.9 2.48 71.3 0.93 0.07 Undetermined <=delta >=delta 54 12.3 151.3 2.48 61.0 0.98 0.02 REFERENCE <=delta <delta 55 11.3 127.7 2.48 51.5 1.00 0.00 REFERENCE <=delta <delta 56 10.3 106.1 2.48 42.8 1.00 0.00 REFERENCE <=delta <delta 57 9.3 86.5 2.48 34.9 1.00 0.00 REFERENCE <=delta <delta 58 8.3 68.9 2.48 27.8 1.00 0.00 REFERENCE <=delta <delta 59 7.3 53.3 2.48 21.5 1.00 0.00 REFERENCE <=delta <delta 60 6.3 39.7 2.48 16.0 1.00 0.00 REFERENCE <=delta <delta 61 5.3 28.1 2.48 11.3 1.00 0.00 REFERENCE <=delta <delta 62 4.3 18.5 2.48 7.5 1.00 0.00 REFERENCE <=delta <delta 63 3.3 10.9 2.48 4.4 1.00 0.00 REFERENCE <=delta <delta 64 2.3 5.3 2.48 2.1 1.00 0.00 REFERENCE <=delta <delta 65 1.3 1.7 2.48 0.7 1.00 0.00 REFERENCE <=delta <delta 66 0.3 0.1 2.48 0.0 1.00 0.00 REFERENCE <=delta <delta

### 10 

# References

Bowman, M. F. and K. M. Somers. 2006. Eval uating a novel Test Site Analysis (TSA) bioassessment approach. Journal of th e North American Benthological Society 25:712-727. Gibbons, R.D. 2003. A statistical approach for performing water quality impairment assessments. Journal of the American Water Resources Association 39:841–849. Kilgour, B. W., K. M. Somers, and D. E. Matthews. 1998. Using the normal range as a criterion for ecological significance in e nvironmental monitoring and assessment. Ecoscience 5:542–550. State Water Resources Control Board (SWRCB). 2004. Functional Equivalent Document: Water Quality Control Policy for Developing California s Clean Water Act Section 303(d) List. Califor nia Division of Water Quality. http://www.swrcb.ca.gov/water_issu es/programs/tmdl/303d_policydocs.shtml . See Document #100 on this web site.



# Additional References
Barnett, V. and Bown, M. 2002. Statistically meaningful standards fo r contaminated sites using composite sampling. Environmetrics 13: 1-13. Bowman, M. F., and K. M. Somers. 2005. Considerations when using the reference condition approach for bioassessment of freshwater ecosystems. Water Quality Research Journal of Canada 40:347–360. Goudey, R. 2007. Do statistical inferences allo wing three alternative decision give better feedback for environmentally precautionary decision-making? Journal of Environmental Management 65:338-344. Lin, Pi-Erh, D. Meeter, X.-F. Niu. 2000. A nonparametric procedure for listing and delisting impaired waters based on crit erion exceedances. Technical report submitted to Florida DEP. Parkhurst D.F. 2001. Statistical significance tests: equivalen ce and reverse tests should reduce misinterpretation. BioScience, 51, 1051–1057. Shabman, L. and E. Smith. 2003. Implications of Applying Statistically Based Procedures for Water Quality Assessment. Journa l of Water Resources Planning and Management. Smith, E. P., Ye, K., Hughes, C., and Sh abman, L. 2001. Statistical assessment of violations of water qualit y standards under Section 303d of the Clean Water Act. Environ. Sci. Technol., 35: 606–612. Smith, E. P., Zahran, A., Mahmoud, M., and Ye, K. 2003 . Evaluation of water quality using acceptance sampling by variables. Environmetrics, 14:373-386. Warn, A. E. 1989. Auditing the quality of effl uent discharges. Envi ronmental Monitoring and Assessment 12:165–181.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### A9-1 

## Appendix A9. Mapping Continuous Biological Index Values to BCG Tiers

Cross

- reference:
Appendix B-13. Mapping Continuous Biological Index Values to BCG Tiers . U.S. EPA. 2010. Technical Support Document for U.S. EPA’s Proposed Rule for Numeric Nutrient Criteria for Florida’s Inland Surface Fresh Waters. Water Quality Standards for the State of Florida’s Lakes and Flowing Waters. Proposed Rule . January 14, 2010. E PA

- HQ

- OW -2009-0596, FRL-9105-1.

# Mapping Continuous Biological Index Values to BCG Tiers

## Appendix B-13. Mapping Continuous Biol ogical Index Values to BCG Tiers The Florida Department of Environmental Prot ection (FDEP) conducted a workshop to calibrate a biological condition gradient (BCG) using macroinvertebrate da ta collected from streams in Florida. During the workshop, macroinvertebrate sa mples (a list of taxa and their abundances) from 30 streams spanning the human disturban ce gradient were provi ded to a panel of 22 biologists with expertise and experience with Flor ida streams. Each biologist then assigned each sample to one of the six BCG tiers. A stream c ondition index value (SCI) was also computed for each sample, but this SCI value was not provi ded to the biologists during the calibration workshop.
BCG tiers represent categories of stream condition, ranging from “as naturally occurs” (Tier 1) to “severely degraded” (Tier 6). Modeling the relationship between the SCI value associated with each sample and the BCG tiers assigned to the sa mple requires that one taken into account the categorical nature of the data. One approach for modeling the relations hip between a continuous variable (e.g., SCI) and a categ orical response (e.g., BCG tier) is a proportional odds logistic regression model (Guisan and Harr ell 2000). This model estimates the cumulative probability of a site’s being assigned to a given tier (e.g., Tier 3) or to any higher-quality tier (Tier 1 or 2). The model equation can be written as follows:



### )( 1 ln SCIba g g
j j j


###         

 Eqn. B13-1

Where gj 

### is the probability of a site’s being assigned to Tiers 1 through j 

### , and j 

### ranges from 1 to 5. Note that the regression coefficient, aj 

### , can differ depending on the value of j 

### , but the regression coefficient that scales the effects of SCI is constant fo r all tiers. Five parallel models are fit, modeling the probability of assignment to Tier s 5 to 1, Tiers 4 to 1, Tiers 3 to 1, Tiers 2 to 1, and Tier 1 only. Once these five models are fit, the probability of assi gnment to any single tier can be extracted from the model results.
In Figure B13-1, the mean predictions of a proportional odds logistic regression model are plotted as solid lines. The lines are color-coded and labeled by di fferent tiers, and each line can be interpreted as the proportion of experts that assigned samples with the indicated SCI value to a particular tier. For example, approximately 90% of experts assigned a sample with SCI = 0 to Tier 6 (brown line), while the remaining 10% of experts assigned the sample to Tier 5 (purple line). The solid circles represent the actual expert assignments recorded from the workshop for each SCI value. The size of the ci rcle is proportional to the number of experts that assigned a sample to a particular tier, and the circles are color-coded by tier.
Expert consensus in tier assignments was high for very degraded sites. That is, sites with SCI < 15 were assigned only to Tier 5 or 6. Consequent ly, the maximum values for the curves for Tiers 6 and 5 were relatively high. Similarly, expert consensus was high for high-quality sites because most sites with SCI > 58 were scored as Tier 1 or 2. The range of expert assignments at midrange SCI values was much broader.

### At the workshop, experts were also asked to identify a point on the BCG where they thought streams were impaired, and approximately half th e experts identified the transition between Tier 4 and Tier 5 as the impairment threshold. The ot her half identified the boundary between Tier 3 and Tier 4 as the impairment threshold. Most ex perts also agreed that Tiers 1 and 2 corresponded to reference conditions.
One approach for using the results from the BCG calibration workshop to support a biocriterion threshold established by candidate reference sites is to identify an SCI value that corresponds with both a low probability of assignment to Tier 5 (impaired) and corresponds with a low probability of assignment to Tier 2 ( reference cond itions). For these data, this point occurs at approximately SCI = 40–44, where both the purple and the blue lines are low (i.e., less than about 0.15). In a sense, thresholds selected in this range of values balance the probability of mistakenly assessing a degraded site as meeting aquatic life use goals w ith the probability of mistakenly assessing a refe rence site as impaired.
The same modeling approach can be applied to e xpert assignments of lake vegetation samples to a BCG. Here, lake vegetation can also be summ arized as a Lake Vegetation Index (LVI).
The consensus among experts was somewhat weaker compared to the resu lts for the SCI. For example, most experts assigned the lake with the highest LVI value (LVI = 94) to Tier 1 or 2, but one expert assigned it to Tier 4. Similarly, at the other end of the condition gradient, a lake with LVI = 18 was assigned by experts to Tiers 3–6.
The same logic can be applied to interpreting thes e results to support criter ion selection. In this case, the probabilities of assignment to Tiers 5 and 2 were less than about 0.15 for LVI = 50–58.

Figure B13-1. BCG tier assignments modeled w ith a proportional odds logistic regression.

### Figure B13-2. BCG tier assignments based on lake vegetation. LVI: Lake Vegetation Index.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### B1 -1 

## Appendix B1. Data Supporting EPA’s Approach for Deriving Numeric Nutrient Criteria for Florida Lakes

Appendix B1 contains the data EPA used to derive numeric nutrient criteria for Florida lakes. It is being provided in electronic form on CD. A copy of the CD can be obtained by contacting EPA’s docket office. The docket identification number is EPA- HQ

- OW -2009-0596.
The Office of Water (OW) Docket Center is open from 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding legal holidays. The OW Docket Center telephone number is 202-566-1744 and the Docket address is OW Docket, EPA West, Room 3334, 1301 Constitution Ave., NW, Washington, DC 20004.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### B2 -1 

## Appendix B2. Analysis of Paleolimnological Data

Although naturally eutrophic lakes occur in Florida, and may be common , just because a lake is naturally eutrophic does not necessarily mean that it has not eutrophied further as a result ofhuman activities. Nutrient criteria for naturally eutrophic lakes (most probably developed as sitespecific alternative criteria, or SSAC) should protect the natural state of the lakes and prevent further eutrophication. Hyper-eutrophication of Florida lak es can be manifested as increased dominance of blue- green algae (Riedinger -Whitmore et al. 2005) or as an ecosystem shift from submerged vegetation dominance (transparent water) to extreme phytoplankton dominance (loss of transparency; see , Bachmann et al. 1999, 2002; Lowe et al. 1999, 2001 ; Scheffer et al. 1993, 2001) . EPA examined evidence whether Florida lakes have increased in trophic state since early settlement or development.
EPA reanalyzed the paleolimnological findings reported by Whitmore and Brenner (2002), and Whitmore -Riedinger et al. (2005). Analysis was restricted to the WACALIB inferred TP for 32 lakes reported (Whitmore and Brenner 2002, Whitmore-Riedinger et al. 2005). Total phosphorus concentration of the lakes was inferred from the diatom species composition from surface and 1 m deep samples of sediment cores, from 1 to 4 cores for each lake (Whitmore and Brenner 2002). EPA only used data from the top and bottom of the cores reported in an appendix table of Whitmore and Brenner (2002), plus two additional cores from Whitmore-Riedinger et al. (2005). Intermediate samples between the sediment surface and 1 m depth have also been analyzed by Whitmore and colleagues for a subset of the 32 lakes.
Inferred TP concentrations were log

- transformed ( common logarithm). An appropriate statistical test is the paired t -test of the inferred top and bottom TP concentrations, which is equivalent to a
t -test of the differences, where the null hypothesis is zero difference. To avoid artificially inflating the sample size with multiple cores, the t -test was performed on lake- average differences (N = 32). The results were highly significant —on average, the inferred limnetic TP is higher in surface sediments than in pre-industrial sediments in these lakes. The ave rage difference was 0.132 (common log units), which is equivalent to a 35% increase in TP. There was less than 1% probability of these results being due to chance alone ( p = 0.00167; t 31 

### = 3.44).
Although a 35% increase in TP might not seem particularly large, it is important to remember that 35% is the average estimated increase over all 32 lakes. Several lakes had almost no inferred difference (no change); a small number of lakes actually decreas ed in inferred TP (apparent oligotrophication); and in more than half of the lakes the inferred TP increased, some more than doubling. Table B2-1 shows the breakdown of the magnitudes of apparent TP change.
In summary, inferred TP increased by more than 25% in 59% of the lakes examined, and the inferred TP more than doubled in 19% of lakes. Inferred TP decreased by more than 20% (oligotrophication) in 13% of lakes.Technical Support Document for U.S. EPA’s Final Rule for Numeric Criteria for Nitrogen/Phosphorus Pollution in Florida’s Inland Surface Fresh Waters



### B2 -2 Table B2

- 1. Decrease or increase of inferred TP in 32 lakes.

Magnitude o f Inferred Change, 1m Sediment v s. Surficial Sediment N of L akes % of L akes Notes
Lakes with decreased TP 4 13% Total oligtrophication Decrease by more than 33% 2 6.2% Apparent oligotrophication Decrease by 20

– 33% 2 6.2% Probable oligotrophication Decrease less than 20% and increase less than 25% 9 28% No apparent change in TP Increase by 25

– 50% 6 19% Probable eutrophication Increase by 50

– 100% 7 22% Apparent eutrophication Increase more than 100% ( more than double) 6 13% Severe eutrophication
Lakes with increased TP 19 59% Total eutrophication Source: data analyzed from Whitmore and Brenner (2002) and Whitmore

- Riedinger et al. (2005).



### References Bachmann, R.W., M.V. Hoyer, and D.E. Canfield, Jr. 1999. The restoration of Lake Apopka in relation to alternative stable states. Hydrobiologia 394:219–232.
Bachmann, R.W., C.A. Horsburgh, M.V. Hoyer, L.K. Mataraza, and D.E. Canfield, Jr. 2002. Relations between trophic state indicators and plant biomass in Florida lakes. Hydrobiologia 470:219–234.

Lowe , E.F., L.E. Battoe, M. Coveney, and D. Stites. 1999. Setting water quality goals for restoration of Lake Apopka. Lake and Reservoir Management 15(2):103–120.
Lowe, E.F., L.E. Battoe, M.F. Coveney, C.L. Schelske, K.E. Havens, E.R. Marzolf, and K.R. Reddy. 2001. The restoration of Lake Apopka in relation to alternative stable states: an alternative view to that of Bachmann et al. (1999). Hydrobiologia 448:11–18.
Riedinger -Whitmore, M., T. Whitmore, J. Smoak, M. Brenner, A. Moore, J. Curtis, and C.L. Schelske . 2005. Cyanobacterial proliferation is a recent response to eutrophication in many Florida lakes: a paleolimnological assessment. Lake and Reservoir Management 21:423–435.
Scheffer, M.S., S. Hosper, M.L. Meijer, B. Moss, and E. Jeppesen. 1993. Alternative equilibria in shallow lakes. Trends in Ecology and Evolution 8:275–279.
Scheffer, M., S. Carpenter, J.A. Foley, C. Folke, and B. Walker. 2001. Catastrophic shifts in ecosystems. Nature 413:591–596.
Whitmore, T. and M. Brenner. 2002. Paleologic Characterization of Pre- disturbance Water Quality Conditions in EPA Defined Florida Lake Regions. University of Florida, Department of Fisheries and Aquatic Sciences, Gainesville, FL.