# Patent

## Title
5′ CpG nucleic acids and methods of use

## Abstract

The invention relates to a class of CpG immunostimulatory oligonucleotides containing a 5′TCG motif or a CG at or near the 5′ end that are useful for stimulating an immune response.

## Background
RELATED APPLICATIONS
This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application Ser. No. 60/432,409, filed Dec. 11, 2002 and U.S. Ser. No. 60/506,108 filed Sep. 25, 2003, which are herein incorporated by reference in their entirety.


FIELD OF THE INVENTION
The present invention relates generally to immunostimulatory nucleic acids, compositions thereof and methods of using the immunostimulatory nucleic acids.
BACKGROUND OF THE INVENTION
Bacterial DNA has immune stimulatory effects to activate B cells and natural killer cells, but vertebrate DNA does not (Tokunaga, T., et al., 1988. Jpn. J. Cancer Res. 79:682-686; Tokunaga, T., et al., 1984, JNCI 72:955-962; Messina, J. P., et al., 1991, J. Immunol. 147:1759-1764; and reviewed in Krieg, 1998, In: Applied Oligonucleotide Technology, C. A. Stein and A. M. Krieg, (Eds.), John Wiley and Sons, Inc., New York, N.Y., pp. 431-448) and Krieg. A. M. CpG motifs in bacterial DNA and their immune effects (2002) Annu. Rev. Immunol. 20: 709-760. It is now understood that these immune stimulatory effects of bacterial DNA are a result of the presence of unmethylated CpG dinucleotides in particular base contexts (CpG motifs), which are common in bacterial DNA, but methylated and underrepresented in vertebrate DNA (Krieg et al, 1995 Nature 374:546-549; Krieg, 1999 Biochim. Biophys. Acta 93321:1-10). The immune stimulatory effects of bacterial DNA can be mimicked with synthetic oligodeoxynucleotides (ODN) containing these CpG motifs. Such CpG ODN have highly stimulatory effects on human and murine leukocytes, inducing B cell proliferation; cytokine and immunoglobulin secretion; natural killer (NK) cell lytic activity and IFN-γ secretion; and activation of dendritic cells (DCs) and other antigen presenting cells to express costimulatory molecules and secrete cytokines, especially the Th1-like cytokines that are important in promoting the development of Th1-like T cell responses. These immune stimulatory effects of native phosphodiester backbone CpG ODN are highly CpG specific in that the effects are dramatically reduced if the CpG motif is methylated, changed to a GpC, or otherwise eliminated or altered (Krieg et al, 1995 Nature 374:546-549; Hartmann et al, 1999 Proc. Natl. Acad. Sci USA 96:9305-10).
In early studies, it was thought that the immune stimulatory CpG motif followed the formula purine-purine-CpG-pyrimidine-pyrimidine (Krieg et al, 1995 Nature 374:546-549; Pisetsky, 1996 J. Immunol. 156:421-423; Hacker et al., 1998 EMBO J. 17:6230-6240; Lipford et al, 1998 Trends in Microbiol. 6:496-500). However, it is now clear that mouse lymphocytes respond quite well to phosphodiester CpG motifs that do not follow this “formula” (Yi et al., 1998 J. Immunol. 160:5898-5906) and the same is true of human B cells and dendritic cells (Hartmann et al, 1999 Proc. Natl. Acad. Sci USA 96:9305-10; Liang, 1996 J. Clin. Invest. 98:1119-1129). Nevertheless, the term “CpG motif” is generally used to refer to a hexamer motif in which the CpG dinucleotide is located at the center.
SUMMARY OF THE INVENTION
The invention involves the finding that specific sub-classes of CpG immunostimulatory oligonucleotides having a 5′CpG are highly effective in mediating immune stimulatory effects. These CpG nucleic acids are useful therapeutically and prophylactically for stimulating the immune system to treat cancer, infectious diseases, allergy, asthma and other disorders and to help protect against opportunistic infections following cancer chemotherapy. The strong yet balanced, cellular and humoral immune responses that result from CpG stimulation reflect the body's own natural defense system against invading pathogens and cancerous cells.
In particular, immunostimulatory CpG containing oligonucleotides having a 5′TCG motif, rather than the conventional hexamer motif have important therapeutic properties. It has been discovered that oligonucleotides having a ′5TCG motif without any additional unmethylated CpG motifs have strong immunostimulatory capability. In one aspect the invention is a composition comprising an oligonucleotide: 5′TCGX1X2N13′, wherein N1 is 2-95 nucleotides and, when X1 is C or A, X2 is A, T, or C (SEQ. ID NO.: 61); when X1 is T, X2 is A or G (SEQ. ID NO.: 62); and when X1 is G, X2 is any nucleotide (SEQ. ID NO.: 63).
The invention, in other aspects, relates to an oligonucleotide comprising 5′TCGTN13′ (SEQ. ID NO.: 64). In the oligonucleotide N1 is 3-96 nucleotides, but when N1 is 16 nucleotides N1 does not include a C12 (5′-CCCCCCCCCCC-3′ SEQ. ID NO.: 65), and when N1 is 8 nucleotides N1 is at least 50% C or 70% T (SEQ. ID NO.: 66).
According to other aspects, an oligonucleotide comprising 5′TCGAN13′ (SEQ. ID NO.: 67) is provided. In the oligonucleotide N1 is 3-96 nucleotides, but when N1 is 19 nucleotides N1 is at least 55% pyrimidine (SEQ. ID NO.: 68), and when N1 is 8 nucleotides N1 is at least 50% T or C (SEQ. ID NO.: 69).
According to other aspects, an oligonucleotide comprising 5′TCGN13′ is provided. In the oligonucleotide N1 is 10-96 nucleotides, and the C content of the oligonucleotide is less than or equal to 60%, and the A content of the oligonucleotide is less than or equal to 30%.
According to other aspects, an oligonucleotide is provided that comprises 5′TYZN13′. In the oligonucleotide N1 is 4-97 nucleotides, and the oligonucleotide does not include an unmethylated CG motif. Y is a cytosine or modified cystosine. Z is a guanine or modified guanine. In one embodiment Y is 5′methyl cytosine, 5-methyl-deoxycytosine, 5-methyl-deoxyisocytosine, 5-hydroxy-deoxycytosine, deoxyuridine, N4-ethyl-deoxycytosine, 2′-deoxyuridine, 5-fluoro-2′-dU, and dSpacer. In other embodiments Z is 7-deazaguanine, 7-deaza-7-substituted guanine (such as 7-deaza-7-(C2-C6)alkynylguanine), 7-deaza-8-substituted guanine, hypoxanthine, 2,6-diaminopurine, 2-aminopurine, purine, 8-substituted guanine such as 8-hydroxyguanine, and 6-thioguanine, Inosine, 2-aminopurine, nebularine, and dSpacer.
In the oligonucleotide formulas 5′ refers to the free 5′ end of the oligonucleotide and 3′ refers to the free 3′ end of the oligonucleotide.
In some embodiment the oligonucleotide has one of the following structures: 5′ T*C*G*A*G*G*A*C*T*T*C*T*C*T*C*A*G*G*T*T 3′ (SEQ. ID NO.: 50) or 5′ T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*T 3′ (SEQ. ID NO.: 2) The * refers to a phosphorothioate linkage.
According to one embodiment the oligonucleotide includes at least 1 modified internucleotide linkage. In other embodiments the oligonucleotide includes at least 50% modified internucleotide linkages. Optionally all internucleotide linkages of the oligonucleotide are modified. The stabilized internucleotide linkage may be a phosphorothioate linkage.
In some embodiments the oligonucleotide is 20-100 nucleotides in length. In other embodiments it is 40 or less nucleotides in length.
N1 is free of unmethylated CG motifs. N1 may be defined by N2N3, such that N2 is 8-94 nucleotides, or in some embodiments 8-40 nucleotides, and N3 is 2-5 pyrimidines. In some embodiments N3 is TTTTT, TTTT, TTT, or TT. N1, according to other embodiments, may be at least 50% pyrimidine or at least 80% pyrimidine. In yet other embodiments N1 is free of Poly-A and Poly-G sequences. In other embodiments N1 is TN2 and N2 is 8-94 nucleotides.
The invention involves, in one aspect, the discovery that the 5′ sequence of immunostimulatory nucleotides, their length and internucleotide linkage have specific influences on the cytokine profile of the induced immune response and that these discoveries can be used to design a subset of CpG immunostimulatory oligonucleotides that have improved immune stimulatory properties. The preferred CpG immunostimulatory oligonucleotides fall within one of the following 6 general formulas: 5′-X1YRM1-3′, 5′-X2CGM2-3′, 5′-X3CGM3-3′, 5′-X4CGM4-3′, 5′-X5CGM5-3′ and 5′-TTGM6-3′. The formulas define subsets of the class of CpG oligonucleotides which demonstrated excellent immune stimulating properties and yet do not include additional unmethylated CpG motifs. In the formulas 5′ refers to the free 5′ end of the oligonucleotide and 3′ refers to the free 3′ end of the oligonucleotide.
In one aspect of the invention the ODN has the general formula 5′-X1YRM1-3′, wherein X1 is a single nucleotide; Y is a cytosine or a modified cytosine; R is a guanine or a modified guanine; and M1 is a nucleic acid of 1-3 nucleotides. According to other embodiments of the invention, the internucleotide linkages of the oligonucleotide are all stabilized phosphorothioate internucleotide linkages. In one embodiment, the internucleotide linkage between Y and R is a phosphodiester linkage in an Rp configuration. In some embodiments of the invention, the modified cytosine has a C5 substitution and/or the modified guanine has a C8 or C7 substitution. In certain embodiments of the invention, the substituted or modified C or G is selected from the group consisting of 5-substituted cytosines (e.g. 5-methyl-cytosine, 5-fluoro-cytosine, 5-chloro-cytosine, 5-bromo-cytosine, 5-iodo-cytosine, 5-hydroxy-cytosine, 5-hydroxymethyl-cytosine, 5-difluoromethyl-cytosine, and unsubstituted or substituted 5-alkynyl-cytosine), 6-substituted cytosines, N4-substituted cytosines (e.g. N4-ethyl-cytosine), 5-aza-cytosine, 2-mercapto-cytosine, isocytosine, pseudo-isocytosine, cytosine analogs with condensed ring systems (e.g. N,N′-propylene cytosine or phenoxazine), and uracil and its derivatives (e.g. 5-fluoro-uracil, 5-bromo-uracil, 5-bromovinyl-uracil, 4-thio-uracil, 5-hydroxy-uracil, 5-propynyl-uracil), thymine derivatives (e.g. 2-thiothymine, 4-thiothymine, 6-substituted thymines), 7-deazaguanine, 7-deaza-7-substituted guanine (such as 7-deaza-7-(C2-C6)alkynylguanine), 7-deaza-8-substituted guanine, 7-deaza-8-aza guanine, hypoxanthine, N2-substituted guanines (e.g. N2-methyl-guanine), 5-amino-3-methyl-3H,6H-thiazolo[4,5-d]pyrimidine-2,7-dione, 2,6-diaminopurine, 2-aminopurine, purine, indole, adenine, substituted adenines (e.g. N6-methyl-adenine, 8-oxo-adenine) 8-substituted guanine (e.g. 8-hydroxyguanine and 8-bromoguanine), and 6-thioguanine. In another embodiment of the invention, the base is substituted by a universal base (e.g. 4-methyl-indole, 5-nitro-indole, 3-nitropyrrole, P-base, and K-base), an aromatic ring system (e.g. benzimidazole or dichloro-benzimidazole, 1-methyl-1H-[1,2,4]triazole-3-carboxylic acid amide) an aromatic ring system (e.g. fluorobenzene or difluorobenzene) and a hydrogen atom (dSpacer). According to one embodiment of the invention the oligonucleotide is associated with a carrier linked to the 3′ end of the oligonucleotide. In some embodiments, the carrier is selected from the group consisting of a microparticle, dendrimer, liposome, cationic complex, and antigen. In yet another embodiment of the invention, the ODN is administered to the subject along with an antigen. In still another embodiment the CpG immunostimulatory oligonucleotides are useful for treating subjects in combination with the administration of a therapeutic protocol to the subject. In some embodiments of the invention, the therapeutic protocol is surgery.
In some embodiments the oligonucleotide is not associated with a carrier. In other embodiments the oligonucleotide is in a multimerized complex. Optionally the multimerized complex includes the oligonucleotide linked by a multimerization unit to a second oligonucleotide. The second oligonucleotide may have the formula 5′-X1YRM1-3′.
In one aspect the immunostimulatory oligonucleotide of the invention has the general formula 5′-X2YRM2-3′ with a multimerization unit linked to the 3′ end of the oligonucleotide. X2 is a nucleic acid that consists of a single nucleotide, or a dinucleotide or a trinucleotide that does not comprise a CG dinucleotide. Y is a cytosine or a modified cytosine. R is a guanine or a modified guanine. M2 is a nucleic acid of 0-27 nucleotides. In some embodiments the immunostimulatory oligonucleotides have the following structures: 5′-TCG-3′, 5′-TCGT-3′, 5′-UCG-3′, 5′-UCGT-3′. In yet another embodiment M2 is free of a CG dinucleotide. According to another embodiment of the invention X2 is a single nucleotide, and X2 is a pyrimidine. According to other embodiments of the invention, the internucleotide linkages of the oligonucleotide are all stabilized phosphodiester internucleotide linkages.
In some embodiments the multimerization unit is a carrier selected from the group consisting of a microparticle, dendrimer, liposome, cationic complex, cholesterol and antigen. In other embodiments the multimerization unit is a linker between the 3′ end of the oligonucleotide and a second oligonucleotide.
In yet another embodiment of the invention, the ODN is administered to the subject along with an antigen. In still another embodiment the CpG immunostimulatory oligonucleotides are useful for treating subjects in combination with the administration of a therapeutic protocol to the subject. In some embodiments of the invention, the therapeutic protocol is surgery.
According to another aspect of the invention the immunostimulatory oligonucleotide has the general formula 5′-X3CGM3-3′, wherein X3 is a single nucleotide that does not comprise a CG dinucleotide; M3 is a nucleic acid of 3-27 nucleotides that is free of a CG dinucleotide, and M3 has at least one of the following properties: is free of a TC dinucleotide, is at least 30% T nucleotides, consists of A, T, and G or is free of a CCTTCC hexamer having at least one modified internucleotide linkage. In some embodiments M3 has at least 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
In another aspect the immunostimulatory oligonucleotide has the general formula 5′-X4CGM4-3′, wherein X4 is a dinucleotide that does not comprise a CG dinucleotide, and M4 is a nucleic acid of 2-26 nucleotides that is free of a CG dinucleotide and it has at least one of the following properties: is free of a TG or a GT dinucleotide, is at least 38% T nucleotides or consists of A and T. In some embodiments M4 has at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% A or T or modified versions thereof.
In yet another aspect the immunostimulatory oligonucleotide has the general formula 5′-X5CGM5-3′, X5 is a trinucleotide that does not comprise a CG dinucleotide; M5 is a nucleic acid of 1-25 nucleotides that is free of a CG dinucleotide, and wherein M5 has at least one of the following properties: is free of a CT dinucleotide and does not include at least one phosphorothioate linkage, is at least 41% T nucleotides, or consists of A and C. In some embodiments M4 has at least 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
According to another aspect of the invention the immunostimulatory oligonucleotide has the general formula 5′-TTGM6-3′, M6 is a nucleic acid that consists of 5-21 nucleotides, wherein M6 does not comprise a CG dinucleotide, wherein M6 is comprised of at least 30% T nucleotides, and wherein said nucleotide is 10-24 nucleotides in length. In some embodiments M4 has at least 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
In some embodiments the oligonucleotide has one of the following structures:









5′-T*C*G*T*T*T*T*T*T*T*T*T*T-3′
(SEQ. ID NO.: 32)



 


5′-T*T*C*G*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 27)


 


T*T*T-3′


 


5′-T*T*T*C*G*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 28)


 


T*T*T-3′.






The symbol * refers to the presence of a stabilized internucleotide linkage and _: refers to the presence of a phosphodiester linkage.
An oligonucleotide comprising:5′-X6CGM7-3′ is provided according to an aspect of the invention. 5′ designates the 5′ end of the oligonucleotide and 3′ designates the 3′ end of the oligonucleotide. X6 is 1-3 nucleotides and does not include a CG dinucleotide. M7 is a nucleic acid of 6-27 nucleotides and includes at least three CG dinucleotides and is at least 50% T nucleotides. In one embodiment M7 is 16-18 nucleotides in length.
In some embodiments M7 includes at least four CG dinucleotides. In other embodiments at least one CG dinucleotide includes a phosphodiester internucleotide linkage. Optionally at least three CG dinucleotides includes a phosphodiester internucleotide linkage. The oligonucleotide may be selected from the group consisting of SEQ ID NO. 33, 34, 35, 36, and 37.
In another aspect the invention is an oligonucleotide comprising: 5‘-’TTGM8-3′ wherein 5′ designates the 5′ end of the oligonucleotide and 3′ designates the 3′ end of the oligonucleotide, wherein M7 is a nucleic acid of 6-18 nucleotides and includes at least one CG dinucleotide and is at least 50% T nucleotides. Optionally M8 is 14 nucleotides in length.
The immunostimulatory oligonucleotides generally have a length in the range of between 3 and 35 nucleotides. In some embodiments the length is in the range of 4-6, 3-32, 6-30, or 10-24 nucleotides or any integer range therebetween.
According to one embodiment the oligonucleotide includes at least 1 modified internucleotide linkage. In other embodiments the oligonucleotide includes at least 50% modified internucleotide linkages. Optionally all internucleotide linkages of the oligonucleotide are modified. The stabilized internucleotide linkage may be a phosphorothioate linkage.
In another aspect, the invention relates to a method for treating allergy or asthma. The method is performed by administering to a subject having or at risk of having allergy or asthma an immunostimulatory CpG oligonucleotide described herein in an effective amount to treat allergy or asthma. In one embodiment the oligonucleotide is administered to a mucosal surface, such as a respiratory tissue. In other embodiments the oligonucleotide is administered in an aerosol formulation. Optionally the oligonucleotide is administered intranasally. In other embodiments the subject has or is at risk of developing allergic asthma.
A method for inducing cytokine production is provided according to another aspect of the invention. The method is performed by administering to a subject an immunostimulatory CpG oligonucleotide described herein in an effective amount to induce a cytokine selected from the group consisting of IP10, IL6, IL 8, IL12, IL18, TNF, IFN-α, chemokines, and IFN-γ.
In another aspect the invention is a composition of the CpG immunostimulatory oligonucleotides described herein in combination with an antigen or other therapeutic compound, such as an anti-microbial agent or an anti-cancer agent. The anti-microbial agent may be, for instance, an anti-viral agent, an anti-parasitic agent, an anti-bacterial agent or an anti-fungal agent.
The composition may optionally include a pharmaceutical carrier and/or be formulated in a delivery device. In some embodiments the delivery device is selected from the group consisting of cationic lipids, cell permeating proteins, and sustained release devices. In one embodiment the sustained release device is a biodegradable polymer or a microparticle.
According to another aspect of the invention a method of stimulating an immune response is provided. The method involves administering a CpG immunostimulatory oligonucleotide to a subject in an amount effective to induce an immune response in the subject. Preferably the CpG immunostimulatory oligonucleotide is administered orally, locally, in a sustained release device, mucosally, systemically, parenterally, or intramuscularly. When the CpG immunostimulatory oligonucleotide is administered to the mucosal surface it may be delivered in an amount effective for inducing a mucosal immune response or a systemic immune response. In preferred embodiments the mucosal surface is an oral, nasal, rectal, vaginal, or ocular surface.
In some embodiments the method includes exposing the subject to an antigen wherein the immune response is an antigen-specific immune response. In some embodiments the antigen is selected from the group consisting of a tumor antigen, a viral antigen, a bacterial antigen, a parasitic antigen and a peptide antigen.
CpG immunostimulatory oligonucleotides are capable of provoking a broad spectrum of immune response. For instance these CpG immunostimulatory oligonucleotides can be used to redirect a Th2 to a Th1 immune response. CpG immunostimulatory oligonucleotides may also be used to activate an immune cell, such as a lymphocyte (e.g., B and T cells), a dendritic cell, and an NK cell. The activation can be performed in vivo, in vitro, or ex vivo, i.e., by isolating an immune cell from the subject, contacting the immune cell with an effective amount to activate the immune cell of the CpG immunostimulatory oligonucleotide and re-administering the activated immune cell to the subject. In some embodiments the dendritic cell presents a cancer antigen. The dendritic cell can be exposed to the cancer antigen ex vivo.
The immune response produced by CpG immunostimulatory oligonucleotides may also result in induction of cytokine production, e.g., production of IP10, IL6, IL 8, IL12, IL18, TNF, IFN-α, chemokines, and IFN-γ.
In still another embodiment, the CpG immunostimulatory oligonucleotides are useful for treating cancer in a subject having or at risk of developing a cancer. The cancer may be selected from the group consisting of biliary tract cancer, breast cancer, cervical cancer, choriocarcinoma, colon cancer, endometrial cancer, gastric cancer, intraepithelial neoplasms, lymphomas, liver cancer, lung cancer (e.g. small cell and non-small cell), melanoma, neuroblastomas, oral cancer, ovarian cancer, pancreatic cancer, prostate cancer, rectal cancer, sarcomas, thyroid cancer, and renal cancer, as well as other carcinomas and sarcomas. In some important embodiments, the cancer is selected from the group consisting of bone cancer, brain and CNS cancer, connective tissue cancer, esophageal cancer, eye cancer, Hodgkin's lymphoma, larynx cancer, oral cavity cancer, skin cancer, and testicular cancer.
CpG immunostimulatory oligonucleotides may also be used for increasing the responsiveness of a cancer cell to a cancer therapy (i.e., an anti-cancer therapy), optionally when the CpG immunostimulatory oligonucleotide is administered in conjunction with an anti-cancer therapy. The anti-cancer therapy may be, for instance, a chemotherapy, a vaccine (e.g., an in vitro primed dendritic cell vaccine or a cancer antigen vaccine) or an immunotherapeutic agent such as an antibody based therapy. This latter therapy may also involve administering an antibody specific for a cell surface antigen of, for example, a cancer cell, wherein the immune response results in antibody dependent cellular cytotoxicity (ADCC). In one embodiment, the antibody may be selected from the group consisting of Ributaxin, Herceptin, Quadramet, Panorex, IDEC-Y2B8, BEC2, C225, Oncolym, SMART M195, ATRAGEN, Ovarex, Bexxar, LDP-03, ior t6, MDX-210, MDX-11, MDX-22, OV103, 3622W94, anti-VEGF, Zenapax, MDX-220, MDX-447, MELIMMUNE-2, MELIMMUNE-1, CEACIDE, Pretarget, NovoMAb-G2, TNT, Gliomab-H, GNI-250, EMD-72000, LymphoCide, CMA 676, Monopharm-C, 4B5, ior egf.r3, ior c5, BABS, anti-FLK-2, MDX-260, ANA Ab, SMART 1D10 Ab, SMART ABL 364 Ab and ImmuRAIT-CEA.
Thus, according to some aspects of the invention, a subject having cancer or at risk of having a cancer is administered a CpG immunostimulatory oligonucleotide and an anti-cancer therapy. In some embodiments, the anti-cancer therapy is selected from the group consisting of a chemotherapeutic agent, an immunotherapeutic agent and a cancer vaccine.
In still another embodiment of the methods directed to treating cancer, the subject may be further administered interferon-α.
In other aspects, the invention is a method for inducing an innate immune response by administering to the subject a CpG immunostimulatory oligonucleotide in an amount effective for activating an innate immune response.
According to another aspect of the invention a method for treating a viral or retroviral infection is provided. The method involves administering to a subject having or at risk of having a viral or retroviral infection, an effective amount for treating the viral or retroviral infection of any of the compositions of the invention. In some embodiments the virus is caused by a hepatitis virus e.g., hepatitis B, hepatitis C, HIV, herpes virus, or papillomavirus.
A method for treating a bacterial infection is provided according to another aspect of the invention. The method involves administering to a subject having or at risk of having a bacterial infection, an effective amount for treating the bacterial infection of any of the compositions of the invention. In one embodiment the bacterial infection is due to an intracellular bacteria.
In another aspect the invention is a method for treating a parasite infection by administering to a subject having or at risk of having a parasite infection, an effective amount for treating the parasite infection of any of the compositions of the invention. In one embodiment the parasite infection is due to an intracellular parasite. In another embodiment the parasite infection is due to a non-helminthic parasite.
In some embodiments the subject is a human and in other embodiments the subject is a non-human vertebrate such as a dog, cat, horse, cow, pig, turkey, goat, fish, monkey, chicken, rat, mouse, or sheep.
In another aspect the invention relates to a method for inducing a TH1 immune response by administering to a subject any of the compositions of the invention in an effective amount to produce a TH1 immune response.
Each of the limitations of the invention can encompass various embodiments of the invention. It is, therefore, anticipated that each of the limitations of the invention involving any one element or combinations of elements can be included in each aspect of the invention.



BRIEF DESCRIPTION OF THE DRAWINGS
The present invention may be more easily and completely understood when taken in conjunction with the accompanying figures.
FIG. 1 is a bar graph depicting the effect of a 5′-TCG motif on the immunostimulatory activity of non-CpG or CpG ODNs through induction of IL-10.
FIG. 2 is a bar graph depicting effect of a 5′-TCG motif on the immunostimulatory activity of non-CpG or CpG ODNs through induction of IFN-α.
FIG. 3 is a bar graph depicting the effect of a 5′TCG on Poly-A and Poly-T sequences.
FIG. 4 is a bar graph depicting the effect of shifting the CpG dinucleotide from the 5′ to the 3′ end of an ODN.
FIG. 5 is a bar graph demonstrating that the length of an ODN has an effect on stimulatory activity in addition to a 5′-TCG.
FIG. 6 is a bar graph depicting the effect of other 5′ modifications in addition to 5′-TCG.
FIG. 7 is a set of bar graphs depicting the effect of a 5′-TCG modification on stimulatory capability of CpG ODNs as shown by different cellular effects: 7A (IL-10 induction) 7B (IFN-α induction) and 7C (IL-6 induction).
FIG. 8 is a set of bar graphs that shows IL-10 secretion induced by ODN with 5′-TCG.
FIG. 9 is a set of bar graphs that shows IL-10 secretion induced by ODN with 5′-TCG and increasing numbers of thymidines.
FIG. 10A is a set of bar graphs that depicts ODN's with a 5′-TCG as the most potent and efficient ODN's to induce a strong Th1-mediated immune response: 10A (IL-10 induction).
FIG. 11 is a set of bar graphs that depicts how the position of CpG dinucleotides in immune stimulatory ODN determines the strength of type I IFN secretion.
FIG. 12 is a set of bar graphs that shows type I IFN secretion induced by short 5′-TCG ODN's.
FIG. 13 is a set of bar graphs that shows the in vitro immune stimulation by a panel of newly generated CpG ODN's according to the observations described herein: 13A (IL-10 induction) and 13B (IFN-α induction).
FIG. 14 is a set of bar graphs depicting B cell stimulation by short CpG ODN's.
FIG. 15 is a bar graph that shows IL-10 induction by a panel of CpG ODN's and which demonstrates that some ODN having phosphodiester linkage between C and G have increased potency.
FIG. 16 is a bar graph that shows IL-10 induction by a panel of CpG ODN's and which demonstrates that some ODN having a modified 5′ TCG induce IL-10.



DETAILED DESCRIPTION
The invention in one aspect involves the finding that specific sub-classes of CpG immunostimulatory oligonucleotides having a 5′TCG are highly effective in mediating immune stimulatory effects. These CpG nucleic acids are useful therapeutically and prophylactically for stimulating the immune system to treat cancer, infectious diseases, allergy, asthma and other disorders and to help protect against opportunistic infections following cancer chemotherapy. The strong yet balanced, cellular and humoral immune responses that result from CpG stimulation reflect the body's own natural defense system against invading pathogens and cancerous cells.
The invention involves, in one aspect, the discovery that a subset of CpG immunostimulatory oligonucleotides have improved immune stimulatory properties. The preferred CpG immunostimulatory oligonucleotides fall within one of the following 5 general formulas: 5′TCGX1X2N13′, 5′TCGTN13′, 5′TCGAN13′, 5′TCGN13′ and 5′TYZN13′ (SEQ. ID NO.: 61-69). X1 and X2 refer to single nucleotides.
The formulas define subsets of the class of CpG oligonucleotides which demonstrated excellent immune stimulating properties and yet do not include additional unmethylated CpG motifs. In the formulas 5′ refers to the free 5′ end of the oligonucleotide and 3′ refers to the free 3′ end of the oligonucleotide.
N1 encompasses a variable set of nucleotide sequences. The nucleotide sequences may range from 2-97 nucleotides in length or any integer range therebetween. The findings of the invention are based in part on the discovery of the importance of the positional effects of CpG or YpZ motif. It has been discovered that oligonucleotides having a 5′TCG or 5′ TYZ without any additional unmethylated CpG motifs therein are strong immunostimulatory capability. The remainder of the oligonucleotide may be any combination of nucleotides or modified nucleotides as long as the 5′ end of the molecule includes the requisite motif.
It has also been discovered that some sequences of N1, when combined with the 5′TCG or 5′ TYZ produce molecules having even greater immunostimulatory activity. For instance, when N1 is at least 50% pyrimidine the oligonucleotide produces enhanced Th1 biased immune induction. In some embodiments N1 is at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100 pyrimidine, e.g. C or T. A pyrimidine is T or C or modified versions thereof. In some embodiments the 3′ most nucleotides of N1 are pyrimidines. For instance the 3′ end may be TTTTT, TTTT, TTT, TT, T, CCCCC, CCCC, CCC, CC, C, CTT, CCTT, or any other possible combination of pyrimidines. In some limited embodiments N1 is free of a C12 (5′-CCCCCCCCCCCC-3′ (SEQ. ID NO.: 65)).
The invention involves, in one aspect, the discovery that the 5′ sequence of immunostimulatory nucleotides, their length and internucleotide linkage have specific influences on the cytokine profile of the induced immune response and that these discoveries can be used to design a subset of CpG immunostimulatory oligonucleotides that have improved immune stimulatory properties. The preferred CpG immunostimulatory oligonucleotides fall within one of the following 6 general formulas: 5′-X1YRM1-3′, 5′-X2CGM2-3′, 5′-X3CGM3-3′, 5′-X4CGM4-3′, 5′-X5CGM5-3′ and 5′-TTGM6-3′.
The formulas define subsets of the class of CpG oligonucleotides which demonstrated excellent immune stimulating properties and yet do not include additional unmethylated CpG motifs. In the formulas 5′ refers to the free 5′ end of the oligonucleotide and 3′ refers to the free 3′ end of the oligonucleotide.
In the preferred embodiment with the general formula 5′-X1YRM1-3′, X1 is a single nucleotide; Y is a cytosine or a modified cytosine; R is a guanine or a modified guanine; and M1 is a nucleic acid of 1-3 nucleotides. For example, such a oligonucleotide can be
In the preferred embodiment with the general formula 5′-X2CGM2-3′, X2 is a nucleic acid that consists of a single nucleotide, or a dinucleotide or a trinucleotide that does not comprise a CG dinucleotide; and M2 is a nucleic acid of 0-27 nucleotides. In some embodiments the oligonucleotides have the following structures: 5′-TCG-3′, 5′-TCGT-3′, 5′-UCG-3′, 5′-UCGT-3′. In other preferred embodiments M2 is free of a CG dinucleotide.
In the preferred embodiment with the general formula 5′-X3CGM3-3′, X3 is a single nucleotide that does not comprise a CG dinucleotide; M3 is a nucleic acid of 3-27 nucleotides that is free of a CG dinucleotide, and M3 has at least one of the following properties: is free of a TC dinucleotide, is at least 30% T nucleotides, consists of A, T, and G or is free of a CCTTCC hexamer having at least one modified internucleotide linkage. In some embodiments M3 has at least 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
In the preferred embodiment with the general formula 5′-X4CGM4-3′, X4 is a dinucleotide that does not comprise a CG dinucleotide, and M4 is a nucleic acid of 2-26 nucleotides that is free of a CG dinucleotide and it has at least one of the following properties: is free of a TG or a GT dinucleotide, is at least 38% T nucleotides or consists of A and T. In some embodiments M4 has at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% A or T or modified versions thereof.
In the preferred embodiment with the general formula 5′-X5CGM5-3′, X5 is a trinucleotide that does not comprise a CG dinucleotide; M5 is a nucleic acid of 1-25 nucleotides that is free of a CG dinucleotide, and wherein M5 has at least one of the following properties: is free of a CT dinucleotide and does not include at least one phosphorothioate linkage, is at least 41% T nucleotides, or consists of A and C. In some embodiments M4 has at least 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
In the preferred embodiment with the general formula 5′-TTGM6-3′, M6 is a nucleic acid that consists of 5-21 nucleotides, wherein M6 does not comprise a CG dinucleotide, wherein M6 is comprised of at least 30% T nucleotides, and wherein said nucleotide is 10-24 nucleotides in length. In some embodiments M4 has at least 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% T or modified versions thereof.
In some embodiment the oligonucleotide has one of the following structures:









5′-T*C*G*T*T*T*T*T*T*T*T*T*T-3′
(SEQ. ID NO.: 32)



 


5′-T*T*C*G*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 27)


 


T*T*T-3′


 


5′-T*T*T*C*G*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 28)


 


T*T*T-3′.






The symbol * refers to the presence of a stabilized internucleotide linkage and refers to the presence of a phosphodiester linkage.
The oligonucleotides may have one or two accessible 5′ ends. Since the importance of the 5′TCG and 5′TYZ motif has been discovered, it also possible to create modified oligonucleotides having two such 5′ ends. This may be achieved, for instance by attaching two oligonucleotides through a 3′-3′ linkage to generate an oligonucleotide having one or two accessible 5′ ends. Such a structure might have a formula such as 5′TCGN1-N1GCT5′ (SEQ. ID NO.: 13). The 3′3′-linkage may be a phosphodiester, phosphorothioate or any other modified internucleoside bridge. Methods for accomplishing such linkages are known in the art. For instance, such linkages have been described in Seliger, H.; et al., Oligonucleotide analogs with terminal 3′-3′- and 5′-5′-internucleotidic linkages as antisense inhibitors of viral gene expression, Nucleosides & Nucleotides (1991), 10(1-3), 469-77 and Jiang, et al., Pseudo-cyclic oligonucleotides: in vitro and in vivo properties, Bioorganic & Medicinal Chemistry (1999), 7(12), 2727-2735.
Additionally, 3′3′-linked ODNs where the linkage between the 3′-terminal nucleosides is not a phosphodiester, phosphorothioate or other modified bridge, can be prepared using an additional spacer, such as tri- or tetra-ethylenglycol phosphate moiety (Durand, M. et al, Triple-helix formation by an oligonucleotide containing one (dA)12 and two (dT)12 sequences bridged by two hexaethylene glycol chains, Biochemistry (1992), 31(38), 9197-204, U.S. Pat. Nos. 5,658,738, and 5,668,265). Alternatively, the non-nucleotidic linker may be derived from ethanediol, propanediol, or from an abasic deoxyribose (dSpacer) unit (Fontanel, Marie Laurence et al., Sterical recognition by T4 polynucleotide kinase of non-nucleosidic moieties 5′-attached to oligonucleotides; Nucleic Acids Research (1994), 22(11), 2022-7) using standard phosphoramidite chemistry. The non-nucleotidic linkers can be incorporated once or multiple times, or combined with each other allowing for any desirable distance between the 3′-ends of the two ODNs to be linked.
The oligonucleotide in some embodiments does not include an unmethylated CG motif, other than the 5′TCG.
In some embodiment the oligonucleotide has one of the following structures: 5′









T*C*G*A*G*G*A*C*T*T*C*T*C*T*C*A*
(SEQ. ID NO.: 50)



 


G*G*T*T,


 


T*C*G*C*C*C*C*C*C*C*C*C*C*C*C*C*
(SEQ. ID NO.: 51)


 


C,


 


T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 13)


 


T*T*T*T*T,


 


T*C*G*U*U*U*U*U*U*U*U*U*U*U*U*U*
(SEQ. ID NO.: 48)


 


U,


 


T*C_G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 25)


 


T,


 


T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 14)


 


T3′.






The symbol * refers to the presence of a stabilized internucleotide linkage and _: refers to the presence of a phosphodiester linkage.
The immunostimulatory oligonucleotides generally have a length in the range of between 7 and 100 nucleotides. In some embodiments the length is in the range of 7-40, 13-100, 13-40, 13-30, 15-50, or 15-30 nucleotides or any integer range therebetween.
In some preferred embodiments the oligonucleotide is associated with a carrier linked to the 3′ end by, but not limited to, the aforementioned linkers and methods. The carrier can be selected from but not limited to the group consisting of microparticles, dendrimers, liposomes, cationic complexes and antigens.
The terms “nucleic acid” and “oligonucleotide” are used interchangeably to mean multiple nucleotides (i.e., molecules comprising a sugar (e.g., ribose or deoxyribose) linked to a phosphate group and to an exchangeable organic base, which is either a substituted pyrimidine (e.g., cytosine (C), thymine (T) or uracil (U)) or a substituted purine (e.g., adenine (A) or guanine (G)). As used herein, the terms “nucleic acid” and “oligonucleotide” refer to oligoribonucleotides as well as oligodeoxyribonucleotides. The terms “nucleic acid” and “oligonucleotide” shall also include polynucleosides (i.e., a polynucleotide minus the phosphate) and any other organic base containing polymer. Nucleic acid molecules can be obtained from existing nucleic acid sources (e.g., genomic or cDNA), but are preferably synthetic (e.g., produced by nucleic acid synthesis).
The terms “nucleic acid” and “oligonucleotide” also encompass nucleic acids or oligonucleotides with substitutions or modifications, such as in the bases and/or sugars. For example, they include nucleic acids having backbone sugars that are covalently attached to low molecular weight organic groups other than a hydroxyl group at the 2′ position and other than a phosphate group or hydroxy group at the 5′ position. Thus modified nucleic acids may include a 2′-O-alkylated ribose group. In addition, modified nucleic acids may include sugars such as arabinose or 2′-fluoroarabinose instead of ribose. Thus the nucleic acids may be heterogeneous in backbone composition thereby containing any possible combination of polymer units linked together such as peptide-nucleic acids (which have an amino acid backbone with nucleic acid bases). Other examples are described in more detail below.
The immunostimulatory oligonucleotides of the instant invention can encompass various chemical modifications and substitutions, in comparison to natural RNA and DNA, involving a phosphodiester internucleoside bridge, a β-D-ribose unit and/or a natural nucleoside base (adenine, guanine, cytosine, thymine, uracil). Examples of chemical modifications are known to the skilled person and are described, for example, in Uhlmann E et al. (1990) Chem Rev 90:543; “Protocols for Oligonucleotides and Analogs” Synthesis and Properties & Synthesis and Analytical Techniques, S. Agrawal, Ed, Humana Press, Totowa, USA 1993; Crooke S T et al. (1996) Annu Rev Pharmacol Toxicol 36:107-129; and Hunziker J et al. (1995) Mod Synth Methods 7:331-417. An oligonucleotide according to the invention may have one or more modifications, wherein each modification is located at a particular phosphodiester internucleoside bridge and/or at a particular β-D-ribose unit and/or at a particular natural nucleoside base position in comparison to an oligonucleotide of the same sequence which is composed of natural DNA or RNA.
For example, the oligonucleotides may comprise one or more modifications and wherein each modification is independently selected from:

    
    
        a) the replacement of a phosphodiester internucleoside bridge located at the 3′ and/or the 5′ end of a nucleoside by a modified internucleoside bridge,
        b) the replacement of phosphodiester bridge located at the 3′ and/or the 5′ end of a nucleoside by a dephospho bridge,
        c) the replacement of a sugar phosphate unit from the sugar phosphate backbone by another unit,
        d) the replacement of a β-D-ribose unit by a modified sugar unit, and
        e) the replacement of a natural nucleoside base by a modified nucleoside base.
    
    


More detailed examples for the chemical modification of an oligonucleotide are as follows.
The oligonucleotides may include modified internucleotide linkages, such as those described in a or b above. These modified linkages may be partially resistant to degradation (e.g., are stabilized). A “stabilized oligonucleotide molecule” shall mean an oligonucleotide that is relatively resistant to in vivo degradation (e.g. via an exo- or endo-nuclease) resulting form such modifications. Oligonucleotides having phosphorothioate linkages, in some embodiments, may provide maximal activity and protect the oligonucleotide from degradation by intracellular exo- and endo-nucleases.
A phosphodiester internucleoside bridge located at the 3′ and/or the 5′ end of a nucleoside can be replaced by a modified internucleoside bridge, wherein the modified internucleoside bridge is for example selected from phosphorothioate, phosphorodithioate, NR1R2-phosphoramidate, boranophosphate, α-hydroxybenzyl phosphonate, phosphate-(C1-C21)-O-alkyl ester, phosphate-[(C6-C12)aryl-(C1-C21)-O-alkyl]ester, (C1-C8)alkylphosphonate and/or (C6-C12)arylphosphonate bridges, (C7-C12)-α-hydroxymethyl-aryl (e.g., disclosed in WO 95/01363), wherein (C6-C12)aryl, (C6-C20)aryl and (C6-C14)aryl are optionally substituted by halogen, alkyl, alkoxy, nitro, cyano, and where R1 and R2 are, independently of each other, hydrogen, (C1-C18)-alkyl, (C6-C20)-aryl, (C6-C14)-aryl-(C1-C8)-alkyl, preferably hydrogen, (C1-C8)-alkyl, preferably (C1-C4)-alkyl and/or methoxyethyl, or R1 and R2 form, together with the nitrogen atom carrying them, a 5-6-membered heterocyclic ring which can additionally contain a further heteroatom from the group O, S and N.
The replacement of a phosphodiester bridge located at the 3′ and/or the 5′ end of a nucleoside by a dephospho bridge (dephospho bridges are described, for example, in Uhlmann E and Peyman A in “Methods in Molecular Biology”, Vol. 20, “Protocols for Oligonucleotides and Analogs”, S. Agrawal, Ed., Humana Press, Totowa 1993, Chapter 16, pp. 355 ff), wherein a dephospho bridge is for example selected from the dephospho bridges formacetal, 3′-thioformacetal, methylhydroxylamine, oxime, methylenedimethylhydrazo, dimethylenesulfone and/or silyl groups.
A sugar phosphate unit (i.e., a β-D-ribose and phosphodiester internucleoside bridge together forming a sugar phosphate unit) from the sugar phosphate backbone (i.e., a sugar phosphate backbone is composed of sugar phosphate units) can be replaced by another unit, wherein the other unit is for example suitable to build up a “morpholino-derivative” oligomer (as described, for example, in Stirchak E P et al. (1989) Nucleic Acids Res 17:6129-41), that is, e.g., the replacement by a morpholino-derivative unit; or to build up a polyamide nucleic acid (“PNA”; as described for example, in Nielsen P E et al. (1994) Bioconjug Chem 5:3-7), that is, e.g., the replacement by a PNA backbone unit, e.g., by 2-aminoethylglycine. The oligonucleotide may have other carbohydrate backbone modifications and replacements, such as peptide nucleic acids with phosphate groups (PHONA), locked nucleic acids (LNA), and oligonucleotides having backbone sections with alkyl linkers or amino linkers. The alkyl linker may be branched or unbranched, substituted or unsubstituted, and chirally pure or a racemic mixture.
A β-ribose unit or a β-D-2′-deoxyribose unit can be replaced by a modified sugar unit, wherein the modified sugar unit is for example selected from β-D-ribose, α-D-2′-deoxyribose, L-2′-deoxyribose, 2′-F-2′-deoxyribose, 2′-F-arabinose, 2′-O-(C1-C6)alkyl-ribose, preferably 2′-O-(C1-C6)alkyl-ribose is 2′-O-methylribose, 2′-O-(C2-C6)alkenyl-ribose, 2′-[O-(C1-C6)alkyl-O-(C1-C6)alkyl]-ribose, 2′-NH2-2′-deoxyribose, β-D-xylofuranose, α-arabinofuranose, 2,4-dideoxy-β-D-erythro-hexo-pyranose, and carbocyclic (described, for example, in Froehler J (1992) Am Chem Soc 114:8320) and/or open-chain sugar analogs (described, for example, in Vandendriessche et al. (1993) Tetrahedron 49:7223) and/or bicyclosugar analogs (described, for example, in Tarkov M et al. (1993) Helv Chim Acta 76:481).
In some embodiments the sugar is 2′-O-methylribose, particularly for one or both nucleotides linked by a phosphodiester or phosphodiester-like internucleoside linkage.
Nucleic acids also include substituted purines and pyrimidines such as C-5 propyne pyrimidine and 7-deaza-7-substituted purine modified bases. Wagner R W et al. (1996) Nat Biotechnol 14:840-4. Purines and pyrimidines include but are not limited to adenine, cytosine, guanine, and thymine, and other naturally and non-naturally occurring nucleobases, substituted and unsubstituted aromatic moieties.
A modified base is any base which is chemically distinct from the naturally occurring bases typically found in DNA and RNA such as T, C, G, A, and U, but which share basic chemical structures with these naturally occurring bases. The modified nucleoside base may be, for example, selected from hypoxanthine, uracil, dihydrouracil, pseudouracil, 2-thiouracil, 4-thiouracil, 5-aminouracil, 5-(C1-C6)-alkyluracil, 5-(C2-C6)-alkenyluracil, 5-(C2-C6)-alkynyluracil, 5-(hydroxymethyl)uracil, 5-chlorouracil, 5-fluorouracil, 5-bromouracil, 5-hydroxycytosine, 5-(C1-C6)-alkylcytosine, 5-(C2-C6)-alkenylcytosine, 5-(C2-C6)-alkynylcytosine, 5-chlorocytosine, 5-fluorocytosine, 5-bromocytosine, N2-dimethylguanine, 2,4-diamino-purine, 8-azapurine, a substituted 7-deazapurine, preferably 7-deaza-7-substituted and/or 7-deaza-8-substituted purine, 5-hydroxymethylcytosine, N4-alkylcytosine, e.g., N4-ethylcytosine, 5-hydroxydeoxycytidine, 5-hydroxymethyldeoxycytidine, N4-alkyldeoxycytidine, e.g., N4-ethyldeoxycytidine, 6-thiodeoxyguanosine, and deoxyribonucleosides of nitropyrrole, C5-propynylpyrimidine, and diaminopurine e.g., 2,6-diaminopurine, inosine, 5-methylcytosine, 2-aminopurine, 2-amino-6-chloropurine, hypoxanthine or other modifications of a natural nucleoside bases. This list is meant to be exemplary and is not to be interpreted to be limiting.
In particular formulas described herein a set of modified bases is defined. For instance the letter Y is used to refer to a nucleotide containing a cytosine or a modified cytosine. A modified cytosine as used herein is a naturally occurring or non-naturally occurring pyrimidine base analog of cytosine which can replace this base without impairing the immunostimulatory activity of the oligonucleotide. Modified cytosines include but are not limited to 5-substituted cytosines (e.g. 5-methyl-cytosine, 5-fluoro-cytosine, 5-chloro-cytosine, 5-bromo-cytosine, 5-iodo-cytosine, 5-hydroxy-cytosine, 5-hydroxymethyl-cytosine, 5-difluoromethyl-cytosine, and unsubstituted or substituted 5-alkynyl-cytosine), 6-substituted cytosines, N4-substituted cytosines (e.g. N4-ethyl-cytosine), 5-aza-cytosine, 2-mercapto-cytosine, isocytosine, pseudo-isocytosine, cytosine analogs with condensed ring systems (e.g. N,N′-propylene cytosine or phenoxazine), and uracil and its derivatives (e.g. 5-fluoro-uracil, 5-bromo-uracil, 5-bromovinyl-uracil, 4-thio-uracil, 5-hydroxy-uracil, 5-propynyl-uracil). Some of the preferred cytosines include 5-methyl-cytosine, 5-fluoro-cytosine, 5-hydroxy-cytosine, 5-hydroxymethyl-cytosine, and N4-ethyl-cytosine. In another embodiment of the invention, the cytosine base is substituted by a universal base (e.g. 3-nitropyrrole, P-base), an aromatic ring system (e.g. fluorobenzene or difluorobenzene) or a hydrogen atom (dSpacer). The letter Z is used to refer to guanine or a modified guanine base. A modified guanine as used herein is a naturally occurring or non-naturally occurring purine base analog of guanine which can replace this base without impairing the immunostimulatory activity of the oligonucleotide. Modified guanines include but are not limited to 7-deazaguanine, 7-deaza-7-substituted guanine (such as 7-deaza-7-(C2-C6)alkynylguanine), 7-deaza-8-substituted guanine, hypoxanthine, N2-substituted guanines (e.g. N2-methyl-guanine), 5-amino-3-methyl-3H,6H-thiazolo[4,5-d]pyrimidine-2,7-dione, 2,6-diaminopurine, 2-aminopurine, purine, indole, adenine, substituted adenines (e.g. N6-methyl-adenine, 8-oxo-adenine) 8-substituted guanine (e.g. 8-hydroxyguanine and 8-bromoguanine), and 6-thioguanine. In another embodiment of the invention, the guanine base is substituted by a universal base (e.g. 4-methyl-indole, 5-nitro-indole, and K-base), an aromatic ring system (e.g. benzimidazole or dichloro-benzimidazole, 1-methyl-1H-[1,2,4]triazole-3-carboxylic acid amide) or a hydrogen atom (dSpacer).
For use in the instant invention, the oligonucleotides of the invention can be synthesized de novo using any of a number of procedures well known in the art. For example, the b-cyanoethyl phosphoramidite method (Beaucage, S. L., and Caruthers, M. H., Tet. Let. 22:1859, 1981); nucleoside H-phosphonate method (Garegg et al., Tet. Let. 27:4051-4054, 1986; Froehler et al., Nucl. Acid. Res. 14:5399-5407, 1986; Garegg et al., Tet. Let. 27:4055-4058, 1986, Gaffney et al., Tet. Let. 29:2619-2622, 1988). These chemistries can be performed by a variety of automated nucleic acid synthesizers available in the market. These oligonucleotides are referred to as synthetic oligonucleotides. An isolated oligonucleotide generally refers to an oligonucleotide which is separated from components which it is normally associated with in nature. As an example, an isolated oligonucleotide may be one which is separated from a cell, from a nucleus, from mitochondria or from chromatin.
The immunostimulatory nucleic acid molecules of the instant invention can have phosphodiester internuclotide linkages. A phosphodiester internucleotide linkage is the type of linkage characteristic of nucleic acids found in nature. As shown in FIG. 20, the phosphodiester internucleotide linkage includes a phosphorus atom flanked by two bridging oxygen atoms and bound also by two additional oxygen atoms, one charged and the other uncharged. Phosphodiester internucleotide linkage is particularly preferred when it is important to reduce the tissue half-life of the oligonucleotide.
A phosphodiester-like internucleotide linkage is a phosphorus-containing bridging group that is chemically and/or diastereomerically similar to phosphodiester. Measures of similarity to phosphodiester include susceptibility to nuclease digestion and ability to activate RNAse H. Thus for example phosphodiester, but not phosphorothioate, oligonucleotides are susceptible to nuclease digestion, while both phosphodiester and phosphorothioate oligonucleotides activate RNAse H. In a preferred embodiment the phosphodiester-like internucleotide linkage is boranophosphate (or equivalently, boranophosphonate) linkage. U.S. Pat. Nos. 5,177,198; 5,859,231; 6,160,109; 6,207,819; Sergueev et al., (1998) J Am Chem Soc 120:9417-27. In another preferred embodiment the phosphodiester-like internucleotide linkage is diasteromerically pure Rp phosphorothioate. It is believed that diasteromerically pure Rp phosphorothioate is more susceptible to nuclease digestion and is better at activating RNAse H than mixed or diastereomerically pure Sp phosphorothioate. Stereoisomers of CpG oligonucleotides are the subject of co-pending U.S. patent application Ser. No. 09/361,575 filed Jul. 27, 1999, and published PCT application PCT/US99/17100 (WO 00/06588). It is to be noted that for purposes of the instant invention, the term “phosphodiester-like internucleotide linkage” specifically excludes phosphorodithioate and methylphosphonate internucleotide linkages.
The immunostimulatory nucleic acid molecules of the instant invention can have chimeric backbone. For purposes of the instant invention, a chimeric backbone refers to a partially stabilized backbone, wherein at least one internucleotide linkage is phosphodiester or phosphodiester-like, and wherein at least one other internucleotide linkage is a stabilized internucleotide linkage, wherein the at least one phosphodiester or phosphodiester-like linkage and the at least one stabilized linkage are different. Since boranophosphonate linkages have been reported to be stabilized relative to phosphodiester linkages, for purposes of the chimeric nature of the backbone, boranophosphonate linkages can be classified either as phosphodiester-like or as stabilized, depending on the context. For example, a chimeric backbone according to the instant invention could in one embodiment include at least one phosphodiester (phosphodiester or phosphodiester-like) linkage and at least one boranophosphonate (stabilized) linkage. In another embodiment a chimeric backbone according to the instant invention could include boranophosphonate (phosphodiester or phosphodiester-like) and phosphorothioate (stabilized) linkages. A “stabilized internucleotide linkage” shall mean an internucleotide linkage that is relatively resistant to in vivo degradation (e.g., via an exo- or endo-nuclease), compared to a phosphodiester internucleotide linkage. Preferred stabilized internucleotide linkages include, without limitation, phosphorothioate, phosphorodithioate, methylphosphonate, and methylphosphorothioate. Other stabilized internucleotide linkages include, without limitation: peptide, alkyl, dephospho, and others as described above.
Modified backbones such as phosphorothioates may be synthesized using automated techniques employing either phosphoramidate or H-phosphonate chemistries. Aryl- and alkyl-phosphonates can be made, e.g., as described in U.S. Pat. No. 4,469,863; and alkylphosphotriesters (in which the charged oxygen moiety is alkylated as described in U.S. Pat. No. 5,023,243 and European Patent No. 092,574) can be prepared by automated solid phase synthesis using commercially available reagents. Methods for making other DNA backbone modifications and substitutions have been described (e.g., Uhlmann, E. and Peyman, A., Chem. Rev. 90:544, 1990; Goodchild, J., Bioconjugate Chem. 1:165, 1990).
CpG phosphorothioate oligonucleotides with strong stimulatory activity in the mouse system tend to show lower activity on human and other non-rodent immune cells. In the examples the development of a potent human CpG motif and the characterization of its effects and mechanisms of action on human PBMC, e.g., B-cells, and plasmacytoid dendritic cells is described. DNA containing these 5′TCG or 5′TYZ CpG motifs strongly stimulated human peripheral blood cells to produce IL-10, IL-6, IP-10 and IFN-α. The 5′TCG containing ODN could be further optimized by selecting ODNs of a particular length. For instance, ODNs of 22 nucleotides in length are more stimulatory than shorter ODN.
It has been discovered according to the invention that the subsets of CpG immunostimulatory oligonucleotides have dramatic immune stimulatory effects on human cells such as PBMC, suggesting that these CpG immunostimulatory oligonucleotides are effective therapeutic agents for human vaccination, cancer immunotherapy, asthma immunotherapy, general enhancement of immune function, enhancement of hematopoietic recovery following radiation or chemotherapy, and other immune modulatory applications.
As used herein, the terms treat, treated, or treating when used with respect to a disorder such as an infectious disease, cancer, allergy, or asthma refers to a prophylactic treatment which increases the resistance of a subject to development of the disease (e.g., to infection with a pathogen) or, in other words, decreases the likelihood that the subject will develop the disease (e.g., become infected with the pathogen) as well as a treatment after the subject has developed the disease in order to fight the disease (e.g., reduce or eliminate the infection) or prevent the disease from becoming worse.
Thus the CpG immunostimulatory oligonucleotides are useful in some aspects of the invention as a vaccine for the treatment of a subject having or at risk of developing allergy or asthma, an infection with an infectious organism or a cancer in which a specific cancer antigen has been identified. The CpG immunostimulatory oligonucleotides can also be given alone without the antigen or allergen for protection against infection, allergy or cancer or may be administered with other therapeutic agents. Repeated doses may allow longer term protection. A subject at risk as used herein is a subject who has any risk of exposure to an infection causing pathogen or a cancer or an allergen or a risk of developing cancer. For instance, a subject at risk may be a subject who is planning to travel to an area where a particular type of infectious agent is found or it may be a subject who through lifestyle or medical procedures is exposed to bodily fluids which may contain infectious organisms or directly to the organism or even any subject living in an area where an infectious organism or an allergen has been identified. Subjects at risk of developing infection also include general populations to which a medical agency recommends vaccination with a particular infectious organism antigen. If the antigen is an allergen and the subject develops allergic responses to that particular antigen and the subject may be exposed to the antigen, i.e., during pollen season, then that subject is at risk of exposure to the antigen. A subject at risk of developing an allergy to asthma includes those subjects that have been identified as having an allergy or asthma but that don't have the active disease during the CpG immunostimulatory oligonucleotide treatment as well as subjects that are considered to be at risk of developing these diseases because of genetic or environmental factors.
A subject at risk of developing a cancer is one who has a high probability of developing cancer. These subjects include, for instance, subjects having a genetic abnormality, the presence of which has been demonstrated to have a correlative relation to a higher likelihood of developing a cancer and subjects exposed to cancer causing agents such as tobacco, asbestos, or other chemical toxins, or a subject who has previously been treated for cancer and is in apparent remission. When a subject at risk of developing a cancer is treated with a CpG immunostimulatory oligonucleotide and optionally an antigen specific for the type of cancer to which the subject is at risk of developing, the subject may be able to kill the cancer cells as they develop. If a tumor begins to form in the subject, the subject will develop an innate immune response or a specific immune response against the tumor antigen.
In addition to the use of the CpG immunostimulatory oligonucleotides for prophylactic treatment, the invention also encompasses the use of the CpG immunostimulatory oligonucleotides for the treatment of a subject having an infection, an allergy, asthma, or a cancer.
A subject having an infection is a subject that has been exposed to an infectious pathogen and has acute or chronic detectable levels of the pathogen in the body. The CpG immunostimulatory oligonucleotides can be used with or without an antigen or other therapeutic to mount an innate or an antigen specific systemic or mucosal immune response that is capable of reducing the level of or eradicating the infectious pathogen. An infectious disease, as used herein, is a disease arising from the presence of a foreign microorganism in the body. It is particularly important to develop effective vaccine strategies and treatments to protect the body's mucosal surfaces, which are the primary site of pathogenic entry.
A subject having an allergy is a subject that is capable of developing an allergic reaction in response to an allergen. An allergy refers to acquired hypersensitivity to a substance (allergen). Allergic conditions include but are not limited to eczema, allergic rhinitis or coryza, hay fever, conjunctivitis, bronchial asthma, allergic asthma, urticaria (hives) and food allergies, and other atopic conditions.
Allergies are generally caused by IgE antibody generation against harmless allergens. The cytokines that are induced by systemic or mucosal administration of CpG immunostimulatory oligonucleotides are predominantly of a class called Th1 (examples are IL-12, IP-10, IFN-α and IFN-γ) and these induce both humoral and cellular immune responses. The other major type of immune response, which is associated with the production of IL-4 and IL-5 cytokines, is termed a Th2 immune response. In general, it appears that allergic diseases are mediated by Th2 type immune responses. Based on the ability of the CpG immunostimulatory oligonucleotides described herein to shift the immune response in a subject from a predominant Th2 (which is associated with production of IgE antibodies and allergy) to a balanced Th2/Th1 response (which is protective against allergic reactions), an effective dose for inducing an immune response of a CpG immunostimulatory oligonucleotide can be administered to a subject to treat asthma and allergy.
Thus, the CpG immunostimulatory oligonucleotides have significant therapeutic utility in the treatment of allergic conditions and asthma. Th2 cytokines, especially IL-4 and IL-5 are elevated in the airways of asthmatic subjects. These cytokines promote important aspects of the asthmatic inflammatory response, including IgE isotope switching, eosinophil chemotaxis and activation and mast cell growth. Th1 cytokines, especially IFN-γ and IL-12, can suppress the formation of Th2 clones and production of Th2 cytokines. Asthma refers to a disorder of the respiratory system characterized by inflammation, narrowing of the airways and increased reactivity of the airways to inhaled agents. Asthma is frequently, although not exclusively associated with atopic or allergic symptoms. Thus, asthma includes allergic asthma and non-allergic asthma.
A subject having a cancer is a subject that has detectable cancerous cells. The cancer may be a malignant or non-malignant cancer. Cancers or tumors include but are not limited to biliary tract cancer; brain cancer; breast cancer; cervical cancer; choriocarcinoma; colon cancer; endometrial cancer; esophageal cancer; gastric cancer; intraepithelial neoplasms; lymphomas; liver cancer; lung cancer (e.g. small cell and non-small cell); melanoma; neuroblastomas; oral cancer; ovarian cancer; pancreas cancer; prostate cancer; rectal cancer; sarcomas; skin cancer; testicular cancer; thyroid cancer; and renal cancer, as well as other carcinomas and sarcomas. In one embodiment the cancer is hairy cell leukemia, chronic myelogenous leukemia, cutaneous T-cell leukemia, multiple myeloma, follicular lymphoma, malignant melanoma, squamous cell carcinoma, renal cell carcinoma, prostate carcinoma, bladder cell carcinoma, or colon carcinoma, CNS cancer, connective tissue cancer, esophageal cancer, eye cancer, Hodgkin's lymphoma, larynx cancer, oral cavity cancer, skin cancer, and testicular cancer, as well as other carcinomas and sarcomas.
A subject shall mean a human or vertebrate animal including but not limited to a dog, cat, horse, cow, pig, sheep, goat, turkey, chicken, primate, e.g., monkey, and fish (aquaculture species), e.g. salmon. Thus, the compounds may be used to treat cancer and tumors, infections, and allergy/asthma in human and non human subjects. Cancer is one of the leading causes of death in companion animals (i.e., cats and dogs).
In the instances when the CpG oligonucleotide is administered with an antigen, the subject may be exposed to the antigen. As used herein, the term exposed to refers to either the active step of contacting the subject with an antigen or the passive exposure of the subject to the antigen in vivo. Methods for the active exposure of a subject to an antigen are well-known in the art. In general, an antigen is administered directly to the subject by any means such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal, or subcutaneous administration. The antigen can be administered systemically or locally. Methods for administering the antigen and the CpG immunostimulatory oligonucleotide are described in more detail below. A subject is passively exposed to an antigen if an antigen becomes available for exposure to the immune cells in the body. A subject may be passively exposed to an antigen, for instance, by entry of a foreign pathogen into the body or by the development of a tumor cell expressing a foreign antigen on its surface.
The methods in which a subject is passively exposed to an antigen can be particularly dependent on timing of administration of the CpG immunostimulatory oligonucleotide. For instance, in a subject at risk of developing a cancer or an infectious disease or an allergic or asthmatic response, the subject may be administered the CpG immunostimulatory oligonucleotide on a regular basis when that risk is greatest, i.e., during allergy season or after exposure to a cancer causing agent. Additionally the CpG immunostimulatory oligonucleotide may be administered to travelers before they travel to foreign lands where they are at risk of exposure to infectious agents. Likewise the CpG immunostimulatory oligonucleotide may be administered to soldiers or civilians at risk of exposure to biowarfare to induce a systemic or mucosal immune response to the antigen when and if the subject is exposed to it.
An antigen as used herein is a molecule capable of provoking an immune response. Antigens include but are not limited to cells, cell extracts, proteins, polypeptides, peptides, polysaccharides, polysaccharide conjugates, peptide and non-peptide mimics of polysaccharides and other molecules, small molecules, lipids, glycolipids, carbohydrates, viruses and viral extracts and muticellular organisms such as parasites and allergens. The term antigen broadly includes any type of molecule which is recognized by a host immune system as being foreign. Antigens include but are not limited to cancer antigens, microbial antigens, and allergens.
A cancer antigen as used herein is a compound, such as a peptide or protein, associated with a tumor or cancer cell surface and which is capable of provoking an immune response when expressed on the surface of an antigen presenting cell in the context of an MHC molecule. Cancer antigens can be prepared from cancer cells either by preparing crude extracts of cancer cells, for example, as described in Cohen, et al., 1994, Cancer Research, 54:1055, by partially purifying the antigens, by recombinant technology, or by de novo synthesis of known antigens. Cancer antigens include but are not limited to antigens that are recombinantly expressed, an immunogenic portion thereof, or a whole tumor or cancer cell. Such antigens can be isolated or prepared recombinantly or by any other means known in the art.
As used herein, the terms “cancer antigen” and “tumor antigen” are used interchangeably to refer to antigens which are differentially expressed by cancer cells and can thereby be exploited in order to target cancer cells. Cancer antigens are antigens which can potentially stimulate apparently tumor-specific immune responses. Some of these antigens are encoded, although not necessarily expressed, by normal cells. These antigens can be characterized as those which are normally silent (i.e., not expressed) in normal cells, those that are expressed only at certain stages of differentiation and those that are temporally expressed such as embryonic and fetal antigens. Other cancer antigens are encoded by mutant cellular genes, such as oncogenes (e.g., activated ras oncogene), suppressor genes (e.g., mutant p53), fusion proteins resulting from internal deletions or chromosomal translocations. Still other cancer antigens can be encoded by viral genes such as those carried on RNA and DNA tumor viruses.
A microbial antigen as used herein is an antigen of a microorganism and includes but is not limited to virus, bacteria, parasites, and fungi. Such antigens include the intact microorganism as well as natural isolates and fragments or derivatives thereof and also synthetic compounds which are identical to or similar to natural microorganism antigens and induce an immune response specific for that microorganism. A compound is similar to a natural microorganism antigen if it induces an immune response (humoral and/or cellular) to a natural microorganism antigen. Such antigens are used routinely in the art and are well known to those of ordinary skill in the art.
Examples of viruses that have been found in humans include but are not limited to: Retroviridae (e.g. human immunodeficiency viruses, such as HIV-1 (also referred to as HTLV-III, LAV or HTLV-III/LAV, or HIV-III; and other isolates, such as HIV-LP: Picornaviridae (e.g. polio viruses, hepatitis A virus; enteroviruses, human Coxsackie viruses, rhinoviruses, echoviruses); Calciviridae (e.g. strains that cause gastroenteritis); Togaviridae (e.g. equine encephalitis viruses, rubella viruses); Flaviridae (e.g. dengue viruses, encephalitis viruses, yellow fever viruses); Coronoviridae (e.g. coronaviruses); Rhabdoviradae (e.g. vesicular stomatitis viruses, rabies viruses); Coronaviridae (e.g. coronaviruses); Rhabdoviridae (e.g. vesicular stomatitis viruses, rabies viruses); Filoviridae (e.g. ebola viruses); Paramyxoviridae (e.g. parainfluenza viruses, mumps virus, measles virus, respiratory syncytial virus); Orthomyxoviridae (e.g. influenza viruses); Bungaviridae (e.g. Hantaan viruses, bunga viruses, phleboviruses and Nairo viruses); Arena viridae (hemorrhagic fever viruses); Reoviridae (e.g. reoviruses, orbiviurses and rotaviruses); Birnaviridae; Hepadnaviridae (Hepatitis B virus); Parvovirida (parvoviruses); Papovaviridae (papilloma viruses, polyoma viruses); Adenoviridae (most adenoviruses); Herpesviridae (herpes simplex virus (HSV) 1 and 2, varicella zoster virus, cytomegalovirus (CMV), herpes virus; Poxviridae (variola viruses, vaccinia viruses, pox viruses); and Iridoviridae (e.g. African swine fever virus); and unclassified viruses (e.g. the agent of delta hepatitis (thought to be a defective satellite of hepatitis B virus), Hepatitis C; Norwalk and related viruses, and astroviruses).
Both gram negative and gram positive bacteria serve as antigens in vertebrate animals. Such gram positive bacteria include, but are not limited to, Pasteurella species, Staphylococci species, and Streptococcus species. Gram negative bacteria include, but are not limited to, Escherichia coli, Pseudomonas species, and Salmonella species. Specific examples of infectious bacteria include but are not limited to, Helicobacter pyloris, Borelia burgdorferi, Legionella pneumophilia, Mycobacteria sps (e.g. M. tuberculosis, M. avium, M. intracellulare, M. kansaii, M. gordonae), Staphylococcus aureus, Neisseria gonorrhoeae, Neisseria meningitidis, Listeria monocytogenes, Streptococcus pyogenes (Group A Streptococcus), Streptococcus agalactiae (Group B Streptococcus), Streptococcus (viridans group), Streptococcus faecalis, Streptococcus bovis, Streptococcus (anaerobic sps.), Streptococcus pneumoniae, pathogenic Campylobacter sp., Enterococcus sp., Haemophilus influenzae, Bacillus antracis, corynebacterium diphtheriae, corynebacterium sp., Erysipelothrix rhusiopathiae, Clostridium perfringers, Clostridium tetani, Enterobacter aerogenes, Klebsiella pneumoniae, Pasturella multocida, Bacteroides sp., Fusobacterium nucleatum, Streptobacillus moniliformis, Treponema pallidium, Treponema pertenue, Leptospira, Rickettsia, and Actinomyces israelli. 
Examples of fungi include Cryptococcus neoformans, Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis, Chlamydia trachomatis, Candida albicans. 
Other infectious organisms (i.e., protists) include Plasmodium spp. such as Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale, and Plasmodium vivax and Toxoplasma gondii. Blood-borne and/or tissues parasites include Plasmodium spp., Babesia microti, Babesia divergens, Leishmania tropica, Leishmania spp., Leishmania braziliensis, Leishmania donovani, Trypanosoma gambiense and Trypanosoma rhodesiense (African sleeping sickness), Trypanosoma cruzi (Chagas' disease), and Toxoplasma gondii. 
Other medically relevant microorganisms have been described extensively in the literature, e.g., see C. G. A Thomas, Medical Microbiology, Bailliere Tindall, Great Britain 1983, the entire contents of which is hereby incorporated by reference.
An allergen refers to a substance (antigen) that can induce an allergic or asthmatic response in a susceptible subject. The list of allergens is enormous and can include pollens, insect venoms, animal dander dust, fungal spores and drugs (e.g. penicillin). Examples of natural, animal and plant allergens include but are not limited to proteins specific to the following genuses: Canine (Canis familiaris); Dermatophagoides (e.g. Dermatophagoides farinae); Felis (Felis domesticus); Ambrosia (Ambrosia artemiisfolia; Lolium (e.g. Lolium perenne or Lolium multiflorum); Cryptomeria (Cryptomeria japonica); Alternaria (Alternaria alternata); Alder; Alnus (Alnus gultinoasa); Betula (Betula verrucosa); Quercus (Quercus alba); Olea (Olea europa); Artemisia (Artemisia vulgaris); Plantago (e.g. Plantago lanceolata); Parietaria (e.g. Parietaria officinalis or Parietaria judaica); Blattella (e.g. Blattella germanica); Apis(e.g. Apis multiflorum); Cupressus (e.g. Cupressus sempervirens, Cupressus arizonica and Cupressus macrocarpa); Juniperus (e.g. Juniperus sabinoides, Juniperus virginiana, Juniperus communis and Juniperus ashei); Thuya (e.g. Thuya orientalis); Chamaecyparis (e.g. Chamaecyparis obtusa); Periplaneta (e.g. Periplaneta americana); Agropyron (e.g. Agropyron repens); Secale (e.g. Secale cereale); Triticum (e.g. Triticum aestivum); Dactylis (e.g. Dactylis glomerata); Festuca (e.g. Festuca elatior); Poa (e.g. Poa pratensis or Poa compressa); Avena (e.g. Avena sativa); Holcus (e.g. Holcus lanatus); Anthoxanthum (e.g. Anthoxanthum odoratum); Arrhenatherum (e.g. Arrhenatherum elatius); Agrostis (e.g. Agrostis alba); Phleum (e.g. Phleum pratense); Phalaris (e.g. Phalaris arundinacea); Paspalum (e.g. Paspalum notatum); Sorghum (e.g. Sorghum halepensis); and Bromus (e.g. Bromus inermis).
The antigen may be substantially purified. The term substantially purified as used herein refers to an antigen, i.e., a polypeptide which is substantially free of other proteins, lipids, carbohydrates or other materials with which it is naturally associated. One skilled in the art can purify polypeptide antigens using standard techniques for protein purification. The substantially pure polypeptide will often yield a single major band on a non-reducing polyacrylamide gel. In the case of partially glycosylated polypeptides or those that have several start codons, there may be several bands on a non-reducing polyacrylamide gel, but these will form a distinctive pattern for that polypeptide. The purity of the polypeptide antigen may also be determined by amino-terminal amino acid sequence analysis. Other types of antigens such as polysaccharides, small molecule, mimics etc are included within the invention and may optionally be substantially pure.
The oligonucleotides of the invention may be administered to a subject with an anti-microbial agent. An anti-microbial agent, as used herein, refers to a naturally-occurring or synthetic compound which is capable of killing or inhibiting infectious microorganisms. The type of anti-microbial agent useful according to the invention will depend upon the type of microorganism with which the subject is infected or at risk of becoming infected. Anti-microbial agents include but are not limited to anti-bacterial agents, anti-viral agents, anti-fungal agents and anti-parasitic agents. Phrases such as “anti-infective agent”, “anti-bacterial agent”, “anti-viral agent”, “anti-fungal agent”, “anti-parasitic agent” and “parasiticide” have well-established meanings to those of ordinary skill in the art and are defined in standard medical texts. Briefly, anti-bacterial agents kill or inhibit bacteria, and include antibiotics as well as other synthetic or natural compounds having similar functions. Antibiotics are low molecular weight molecules which are produced as secondary metabolites by cells, such as microorganisms. In general, antibiotics interfere with one or more bacterial functions or structures which are specific for the microorganism and which are not present in host cells. Anti-viral agents can be isolated from natural sources or synthesized and are useful for killing or inhibiting viruses. Anti-fungal agents are used to treat superficial fungal infections as well as opportunistic and primary systemic fungal infections. Anti-parasitic agents kill or inhibit parasites.
Examples of anti-parasitic agents, also referred to as parasiticides useful for human administration include but are not limited to albendazole, amphotericin B, benznidazole, bithionol, chloroquine HCl, chloroquine phosphate, clindamycin, dehydroemetine, diethylcarbamazine, diloxanide furoate, eflornithine, furazolidaone, glucocorticoids, halofantrine, iodoquinol, ivermectin, mebendazole, mefloquine, meglumine antimoniate, melarsoprol, metrifonate, metronidazole, niclosamide, nifurtimox, oxamniquine, paromomycin, pentamidine isethionate, piperazine, praziquantel, primaquine phosphate, proguanil, pyrantel pamoate, pyrimethanmine-sulfonamides, pyrimethanmine-sulfadoxine, quinacrine HCl, quinine sulfate, quinidine gluconate, spiramycin, stibogluconate sodium (sodium antimony gluconate), suramin, tetracycline, doxycycline, thiabendazole, tinidazole, trimethroprim-sulfamethoxazole, and tryparsamide some of which are used alone or in combination with others.
Antibacterial agents kill or inhibit the growth or function of bacteria. A large class of antibacterial agents is antibiotics. Antibiotics, which are effective for killing or inhibiting a wide range of bacteria, are referred to as broad spectrum antibiotics. Other types of antibiotics are predominantly effective against the bacteria of the class gram-positive or gram-negative. These types of antibiotics are referred to as narrow spectrum antibiotics. Other antibiotics which are effective against a single organism or disease and not against other types of bacteria, are referred to as limited spectrum antibiotics. Antibacterial agents are sometimes classified based on their primary mode of action. In general, antibacterial agents are cell wall synthesis inhibitors, cell membrane inhibitors, protein synthesis inhibitors, nucleic acid synthesis or functional inhibitors, and competitive inhibitors.
Antiviral agents are compounds which prevent infection of cells by viruses or replication of the virus within the cell. There are many fewer antiviral drugs than antibacterial drugs because the process of viral replication is so closely related to DNA replication within the host cell, that non-specific antiviral agents would often be toxic to the host. There are several stages within the process of viral infection which can be blocked or inhibited by antiviral agents. These stages include, attachment of the virus to the host cell (immunoglobulin or binding peptides), uncoating of the virus (e.g. amantadine), synthesis or translation of viral mRNA (e.g. interferon), replication of viral RNA or DNA (e.g. nucleoside analogues), maturation of new virus proteins (e.g. protease inhibitors), and budding and release of the virus.
Nucleotide analogues are synthetic compounds which are similar to nucleotides, but which have an incomplete or abnormal deoxyribose or ribose group. Once the nucleotide analogues are in the cell, they are phosphorylated, producing the triphosphate form which competes with normal nucleotides for incorporation into the viral DNA or RNA. Once the triphosphate form of the nucleotide analogue is incorporated into the growing nucleic acid chain, it causes irreversible association with the viral polymerase and thus chain termination. Nucleotide analogues include, but are not limited to, acyclovir (used for the treatment of herpes simplex virus and varicella-zoster virus), gancyclovir (useful for the treatment of cytomegalovirus), idoxuridine, ribavirin (useful for the treatment of respiratory syncitial virus), dideoxyinosine, dideoxycytidine, zidovudine (azidothymidine), imiquimod, and resimiquimod.
The interferons are cytokines which are secreted by virus-infected cells as well as immune cells. The interferons function by binding to specific receptors on cells adjacent to the infected cells, causing the change in the cell which protects it from infection by the virus. α and β-interferon also induce the expression of Class I and Class II MHC molecules on the surface of infected cells, resulting in increased antigen presentation for host immune cell recognition. α and β-interferons are available as recombinant forms and have been used for the treatment of chronic hepatitis B and C infection. At the dosages which are effective for anti-viral therapy, interferons have severe side effects such as fever, malaise and weight loss.
Anti-viral agents useful in the invention include but are not limited to immunoglobulins, amantadine, interferons, nucleoside analogues, and protease inhibitors. Specific examples of anti-virals include but are not limited to Acemannan; Acyclovir; Acyclovir Sodium; Adefovir; Alovudine; Alvircept Sudotox; Amantadine Hydrochloride; Aranotin; Arildone; Atevirdine Mesylate; Avridine; Cidofovir; Cipamfylline; Cytarabine Hydrochloride; Delavirdine Mesylate; Desciclovir; Didanosine; Disoxaril; Edoxudine; Enviradene; Enviroxime; Famciclovir; Famotine Hydrochloride; Fiacitabine; Fialuridine; Fosarilate; Foscarnet Sodium; Fosfonet Sodium; Ganciclovir; Ganciclovir Sodium; Idoxuridine; Kethoxal; Lamivudine; Lobucavir; Memotine Hydrochloride; Methisazone; Nevirapine; Penciclovir; Pirodavir; Ribavirin; Rimantadine Hydrochloride; Saquinavir Mesylate; Somantadine Hydrochloride; Sorivudine; Statolon; Stavudine; Tilorone Hydrochloride; Trifluridine; Valacyclovir Hydrochloride; Vidarabine; Vidarabine Phosphate; Vidarabine Sodium Phosphate; Viroxime; Zalcitabine; Zidovudine; and Zinviroxime.
Anti-fungal agents are useful for the treatment and prevention of infective fungi. Anti-fungal agents are sometimes classified by their mechanism of action. Some anti-fungal agents function as cell wall inhibitors by inhibiting glucose synthase. These include, but are not limited to, basiungin/ECB. Other anti-fungal agents function by destabilizing membrane integrity. These include, but are not limited to, immidazoles, such as clotrimazole, sertaconzole, fluconazole, itraconazole, ketoconazole, miconazole and voriconacole, as well as FK 463, amphotericin B, BAY 38-9502, MK 991, pradimicin, UK 292, butenafine, and terbinafine. Other anti-fungal agents function by breaking down chitin (e.g. chitinase) or immunosuppression (501 cream).
CpG immunostimulatory oligonucleotides can be combined with other therapeutic agents such as adjuvants to enhance immune responses. The CpG immunostimulatory oligonucleotide and other therapeutic agent may be administered simultaneously or sequentially. When the other therapeutic agents are administered simultaneously they can be administered in the same or separate formulations, but are administered at the same time. The other therapeutic agents are administered sequentially with one another and with CpG immunostimulatory oligonucleotide, when the administration of the other therapeutic agents and the CpG immunostimulatory oligonucleotide is temporally separated. The separation in time between the administration of these compounds may be a matter of minutes or it may be longer. Other therapeutic agents include but are not limited to adjuvants, cytokines, antibodies, antigens, etc.
The compositions of the invention may also be administered with non-nucleic acid adjuvants. A non-nucleic acid adjuvant is any molecule or compound except for the CpG immunostimulatory oligonucleotides described herein which can stimulate the humoral and/or cellular immune response. Non-nucleic acid adjuvants include, for instance, adjuvants that create a depo effect, immune stimulating adjuvants, and adjuvants that create a depo effect and stimulate the immune system.
The CpG immunostimulatory oligonucleotides are also useful as mucosal adjuvants. It has previously been discovered that both systemic and mucosal immunity are induced by mucosal delivery of CpG nucleic acids. Thus, the oligonucleotides may be administered in combination with other mucosal adjuvants.
Immune responses can also be induced or augmented by the co-administration or co-linear expression of cytokines (Bueler & Mulligan, 1996; Chow et al., 1997; Geissler et al., 1997; Iwasaki et al., 1997; Kim et al., 1997) or co-stimulatory molecules such as B7 (Iwasaki et al., 1997; Tsuji et al., 1997) with the CpG immunostimulatory oligonucleotides. The term cytokine is used as a generic name for a diverse group of soluble proteins and peptides which act as humoral regulators at nano- to picomolar concentrations and which, either under normal or pathological conditions, modulate the functional activities of individual cells and tissues. These proteins also mediate interactions between cells directly and regulate processes taking place in the extracellular environment. Examples of cytokines include, but are not limited to IP-10, IL-1, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12, IL-15, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), interferon-γ (γ-IFN), IFN-α, tumor necrosis factor (TNF), TGF-β, FLT-3 ligand, and CD40 ligand. In addition to cytokines the CpG oligonucleotides may be used in combination with antibodies against certain cytokines, such as anti-IL-10 and anti-TGF-β, as well as Cox inhibitors, i.e. COX-1 and COX-2 inhibitors.
The oligonucleotides are also useful for redirecting an immune response from a Th2 immune response to a Th1 immune response. This results in the production of a relatively balanced Th1/Th2 environment. Redirection of an immune response from a Th2 to a Th1 immune response can be assessed by measuring the levels of cytokines produced in response to the nucleic acid (e.g., by inducing monocytic cells and other cells to produce Th1 cytokines, including IL-12, IFN-γ and GM-CSF). The redirection or rebalance of the immune response from a Th2 to a Th1 response is particularly useful for the treatment of asthma. For instance, an effective amount for treating asthma can be that amount; useful for redirecting a Th2 type of immune response that is associated with asthma to a Th1 type of response or a balanced Th1/Th2 environment. Th2 cytokines, especially IL-4 and IL-5 are elevated in the airways of asthmatic subjects. The CpG immunostimulatory oligonucleotides described herein cause an increase in Th1 cytokines which helps to rebalance the immune system, preventing or reducing the adverse effects associated with a predominately Th2 immune response.
The CpG immunostimulatory oligonucleotides have the unique capability to promote cell survival, differentiation, activation and maturation of dendritic cells, and are useful for in vitro, in vivo, and ex vivo methods involving dendritic cells.
CpG immunostimulatory oligonucleotides also increase natural killer cell lytic activity and antibody dependent cellular cytotoxicity (ADCC). ADCC can be performed using a CpG immunostimulatory oligonucleotide in combination with an antibody specific for a cellular target, such as a cancer cell. When the CpG immunostimulatory oligonucleotide is administered to a subject in conjunction with the antibody the subject's immune system is induced to kill the tumor cell. The antibodies useful in the ADCC procedure include antibodies which interact with a cell in the body. Many such antibodies specific for cellular targets have been described in the art and many are commercially available.
The CpG immunostimulatory oligonucleotides may also be administered in conjunction with an anti-cancer therapy. Anti-cancer therapies include cancer medicaments, radiation and surgical procedures. As used herein, a “cancer medicament” refers to an agent which is administered to a subject for the purpose of treating a cancer. As used herein, “treating cancer” includes preventing the development of a cancer, reducing the symptoms of cancer, and/or inhibiting the growth of an established cancer. In other aspects, the cancer medicament is administered to a subject at risk of developing a cancer for the purpose of reducing the risk of developing the cancer. Various types of medicaments for the treatment of cancer are described herein. For the purpose of this specification, cancer medicaments are classified as chemotherapeutic agents, immunotherapeutic agents, cancer vaccines, hormone therapy, and biological response modifiers.
Additionally, the methods of the invention are intended to embrace the use of more than one cancer medicament along with the CpG immunostimulatory oligonucleotides. As an example, where appropriate, the CpG immunostimulatory oligonucleotides may be administered with both a chemotherapeutic agent and an immunotherapeutic agent. Alternatively, the cancer medicament may embrace an immunotherapeutic agent and a cancer vaccine, or a chemotherapeutic agent and a cancer vaccine, or a chemotherapeutic agent, an immunotherapeutic agent and a cancer vaccine all administered to one subject for the purpose of treating a subject having a cancer or at risk of developing a cancer.
The chemotherapeutic agent may be selected from the group consisting of methotrexate, vincristine, adriamycin, cisplatin, non-sugar containing chloroethylnitrosoureas, 5-fluorouracil, mitomycin C, bleomycin, doxorubicin, dacarbazine, taxol, fragyline, Meglamine GLA, valrubicin, carmustaine and poliferposan, MMI270, BAY 12-9566, RAS farnesyl transferase inhibitor, farnesyl transferase inhibitor, MMP, MTA/LY231514, LY264618/Lometexol, Glamolec, CI-994, TNP-470, Hycamtin/Topotecan, PKC412, Valspodar/PSC833, Novantrone/Mitroxantrone, Metaret/Suramin, Batimastat, E7070, BCH-4556, CS-682, 9-AC, AG3340, AG3433, Incel/VX-710, VX-853, ZD0101, ISI641, ODN 698, TA 2516/Marmistat, BB2516/Marmistat, CDP 845, D2163, PD183805, DX8951f, Lemonal DP 2202, FK 317, Picibanil/OK-432, AD 32/Valrubicin, Metastron/strontium derivative, Temodal/Temozolomide, Evacet/liposomal doxorubicin, Yewtaxan/Paclitaxel, Taxol/Paclitaxel, Xeload/Capecitabine, Furtulon/Doxifluridine, Cyclopax/oral paclitaxel, Oral Taxoid, SPU-077/Cisplatin, HMR 1275/Flavopiridol, CP-358 (774)/EGFR, CP-609 (754)/RAS oncogene inhibitor, BMS-182751/oral platinum, UFT (Tegafur/Uracil), Ergamisol/Levamisole, Eniluracil/776C85/5FU enhancer, Campto/Levamisole, Camptosar/Irinotecan, Tumodex/Ralitrexed, Leustatin/Cladribine, Paxex/Paclitaxel, Doxil/liposomal doxorubicin, Caelyx/liposomal doxorubicin, Fludara/Fludarabine, Pharmarubicin/Epirubicin, DepoCyt, ZD1839, LU 79553/Bis-Naphtalimide, LU 103793/Dolastain, Caetyx/liposomal doxorubicin, Gemzar/Gemcitabine, ZD 0473/Anormed, YM 116, lodine seeds, CDK4 and CDK2 inhibitors, PARP inhibitors, D4809/Dexifosamide, Ifes/Mesnex/Ifosamide, Vumon/Teniposide, Paraplatin/Carboplatin, Plantinol/cisplatin, Vepeside/Etoposide, ZD 9331, Taxotere/Docetaxel, prodrug of guanine arabinoside, Taxane Analog, nitrosoureas, alkylating agents such as melphelan and cyclophosphamide, Aminoglutethimide, Asparaginase, Busulfan, Carboplatin, Chlorombucil, Cytarabine HCl, Dactinomycin, Daunorubicin HCl, Estramustine phosphate sodium, Etoposide (VP16-213), Floxuridine, Fluorouracil (5-FU), Flutamide, Hydroxyurea (hydroxycarbamide), Ifosfamide, Interferon Alfa-2a, Alfa-2b, Leuprolide acetate (LHRH-releasing factor analogue), Lomustine (CCNU), Mechlorethamine HCl (nitrogen mustard), Mercaptopurine, Mesna, Mitotane (o.p′-DDD), Mitoxantrone HCl, Octreotide, Plicamycin, Procarbazine HCl, Streptozocin, Tamoxifen citrate, Thioguanine, Thiotepa, Vinblastine sulfate, Amsacrine (m-AMSA), Azacitidine, Erthropoietin, Hexamethylmelamine (HMM), Interleukin 2, Mitoguazone (methyl-GAG; methyl glyoxal bis-guanylhydrazone; MGBG), Pentostatin (2′deoxycoformycin), Semustine (methyl-CCNU), Teniposide (VM-26) and Vindesine sulfate, but it is not so limited.
The immunotherapeutic agent may be selected from the group consisting of Ributaxin, Herceptin, Quadramet, Panorex, IDEC-Y2B8, BEC2, C225, Oncolym, SMART M195, ATRAGEN, Ovarex, Bexxar, LDP-03, ior t6, MDX-210, MDX-11, MDX-22, OV103, 3622W94, anti-VEGF, Zenapax, MDX-220, MDX-447, MELIMMUNE-2, MELIMMUNE-1, CEACIDE, Pretarget, NovoMAb-G2, TNT, Gliomab-H, GNI-250, EMD-72000, LymphoCide, CMA 676, Monopharm-C, 4B5, ior egf.r3, ior c5, BABS, anti-FLK-2, MDX-260, ANA Ab, SMART ID10 Ab, SMART ABL 364 Ab and ImmuRAIT-CEA, but it is not so limited.
The cancer vaccine may be selected from the group consisting of EGF, Anti-idiotypic cancer vaccines, Gp75 antigen, GMK melanoma vaccine, MGV ganglioside conjugate vaccine, Her2/neu, Ovarex, M-Vax, O-Vax, L-Vax, STn-KHL theratope, BLP25 (MUC-1), liposomal idiotypic vaccine, Melacine, peptide antigen vaccines, toxin/antigen vaccines, MVA-based vaccine, PACIS, BCG vacine, TA-HPV, TA-CIN, DISC-virus and ImmuCyst/TheraCys, but it is not so limited.
The use of CpG immunostimulatory oligonucleotides in conjunction with immunotherapeutic agents such as monoclonal antibodies is able to increase long-term survival through a number of mechanisms including significant enhancement of ADCC (as discussed above), activation of natural killer (NK) cells and an increase in IFNα levels. The nucleic acids when used in combination with monoclonal antibodies serve to reduce the dose of the antibody required to achieve a biological result.
The invention also includes methods for inducing antigen non-specific innate immune activation and broad spectrum resistance to infectious challenge using the CpG immunostimulatory oligonucleotides. The term innate immune activation as used herein refers to the activation of immune cells other than memory B cells and for instance can include the activation of NK cells, T cells and/or other immune cells that can respond in an antigen independent fashion. A broad spectrum resistance to infectious challenge is induced because the immune cells are in active form and are primed to respond to any invading compound or microorganism. The cells do not have to be specifically primed against a particular antigen. This is particularly useful in biowarfare, and the other circumstances described above such as travelers.
The CpG immunostimulatory oligonucleotides may be directly administered to the subject or may be administered in conjunction with a nucleic acid delivery complex. A nucleic acid delivery complex shall mean a nucleic acid molecule associated with (e.g. ionically or covalently bound to; or encapsulated within) a targeting means (e.g. a molecule that results in higher affinity binding to target cell. Examples of nucleic acid delivery complexes include nucleic acids associated with a sterol (e.g. cholesterol), a lipid (e.g. a cationic lipid, virosome or liposome), or a target cell specific binding agent (e.g. a ligand recognized by target cell specific receptor). Preferred complexes may be sufficiently stable in vivo to prevent significant uncoupling prior to internalization by the target cell. However, the complex can be cleavable under appropriate conditions within the cell so that the oligonucleotide is released in a functional form.
The CpG immunostimulatory oligonucleotide and/or the antigen and/or other therapeutics may be administered alone (e.g., in saline or buffer) or using any delivery vehicles known in the art. For instance the following delivery vehicles have been described: Cochleates (Gould-Fogerite et al., 1994, 1996); Emulsomes (Vancott et al., 1998, Lowell et al., 1997); ISCOMs (Mowat et al., 1993, Carlsson et al., 1991, Hu et., 1998, Morein et al., 1999); Liposomes (Childers et al., 1999, Michalek et al., 1989, 1992, de Haan 1995a, 1995b); Live bacterial vectors (e.g., Salmonella, Escherichia coli, Bacillus calmatte-guerin, Shigella, Lactobacillus) (Hone et al., 1996, Pouwels et al., 1998, Chatfield et al., 1993, Stover et al., 1991, Nugent et al., 1998); Live viral vectors (e.g., Vaccinia, adenovirus, Herpes Simplex) (Gallichan et al., 1993, 1995, Moss et al., 1996, Nugent et al., 1998, Flexner et al., 1988, Morrow et al., 1999); Microspheres (Gupta et al., 1998, Jones et al., 1996, Maloy et al., 1994, Moore et al., 1995, O'Hagan et al., 1994, Eldridge et al., 1989); Nucleic acid vaccines (Fynan et al., 1993, Kuklin et al., 1997, Sasaki et al., 1998, Okada et al., 1997, Ishii et al., 1997); Polymers (e.g. carboxymethylcellulose, chitosan) (Hamajima et al., 1998, Jabbal-Gill et al., 1998); Polymer rings (Wyatt et al., 1998); Proteosomes (Vancott et al., 1998, Lowell et al., 1988, 1996, 1997); Sodium Fluoride (Hashi et al., 1998); Transgenic plants (Tacket et al., 1998, Mason et al., 1998, Haq et al., 1995); Virosomes (Gluck et al., 1992, Mengiardi et al., 1995, Cryz et al., 1998); Virus-like particles (Jiang et al., 1999, Leibl et al., 1998). Other delivery vehicles are known in the art.
The term effective amount of a CpG immunostimulatory oligonucleotide refers to the amount necessary or sufficient to realize a desired biologic effect. For example, an effective amount of a CpG immunostimulatory oligonucleotide administered with an antigen for inducing mucosal immunity is that amount necessary to cause the development of IgA in response to an antigen upon exposure to the antigen, whereas that amount required for inducing systemic immunity is that amount necessary to cause the development of IgG in response to an antigen upon exposure to the antigen. Combined with the teachings provided herein, by choosing among the various active compounds and weighing factors such as potency, relative bioavailability, patient body weight, severity of adverse side-effects and preferred mode of administration, an effective prophylactic or therapeutic treatment regimen can be planned which does not cause substantial toxicity and yet is entirely effective to treat the particular subject. The effective amount for any particular application can vary depending on such factors as the disease or condition being treated, the particular CpG immunostimulatory oligonucleotide being administered the size of the subject, or the severity of the disease or condition. One of ordinary skill in the art can empirically determine the effective amount of a particular CpG immunostimulatory oligonucleotide and/or antigen and/or other therapeutic agent without necessitating undue experimentation.
Subject doses of the compounds described herein for mucosal or local delivery typically range from about 0.1 μg to 10 mg per administration, which depending on the application could be given daily, weekly, or monthly and any other amount of time therebetween or as otherwise required. More typically mucosal or local doses range from about 10 μg to 5 mg per administration, and most typically from about 100 μg to 1 mg, with 2-4 administrations being spaced days or weeks apart. More typically, immune stimulant doses range from 1 μg to 10 mg per administration, and most typically 10 μg to 1 mg, with daily or weekly administrations. Subject doses of the compounds described herein for parenteral delivery for the purpose of inducing an antigen-specific immune response, wherein the compounds are delivered with an antigen but not another therapeutic agent are typically 5 to 10,000 times higher than the effective mucosal dose for vaccine adjuvant or immune stimulant applications, and more typically 10 to 1,000 times higher, and most typically 20 to 100 times higher. Doses of the compounds described herein for parenteral delivery for the purpose of inducing an innate immune response or for increasing ADCC or for inducing an antigen specific immune response when the CpG immunostimulatory oligonucleotides are administered in combination with other therapeutic agents or in specialized delivery vehicles typically range from about 1.0 μg to 100 mg per administration, which depending on the application could be given daily, weekly, or monthly and any other amount of time therebetween or as otherwise required. More typically parenteral doses for these purposes range from about 100 μg to 50 mg per administration, and most typically from about 200 μg to 2 mg, with 2-4 administrations being spaced days or weeks apart. In some embodiments, however, parenteral doses for these purposes may be used in a range of 5 to 10,000 times higher than the typical doses described above.
For any compound described herein the therapeutically effective amount can be initially determined from animal models. A therapeutically effective dose can also be determined from human data for other CpG oligonucleotides which have been tested in humans (human clinical trials are ongoing) and for compounds which are known to exhibit similar pharmacological activities, such as other adjuvants, e.g., LT and other antigens for vaccination purposes. Higher doses may be required for parenteral administration. The applied dose can be adjusted based on the relative bioavailability and potency of the administered compound. Adjusting the dose to achieve maximal efficacy based on the methods described above and other methods as are well-known in the art is well within the capabilities of the ordinarily skilled artisan.
The formulations of the invention are administered in pharmaceutically acceptable solutions, which may routinely contain pharmaceutically acceptable concentrations of salt, buffering agents, preservatives, compatible carriers, adjuvants, and optionally other therapeutic ingredients.
For use in therapy, an effective amount of the CpG immunostimulatory oligonucleotide an/or other therapeutics can be administered to a subject by any mode that delivers the compound to the desired surface, e.g., local, mucosal, systemic. Administering the pharmaceutical composition of the present invention may be accomplished by any means known to the skilled artisan. Preferred routes of administration include but are not limited to oral, parenteral, intramuscular, intranasal, sublingual, intratracheal, inhalation, ocular, vaginal, and rectal.
For oral administration, the compounds (i.e., CpG immunostimulatory oligonucleotides, antigens and/or other therapeutic agents) can be formulated readily by combining the active compound(s) with pharmaceutically acceptable carriers well known in the art. Such carriers enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a subject to be treated. Pharmaceutical preparations for oral use can be obtained as solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone (PVP). If desired, disintegrating agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate. Optionally the oral formulations may also be formulated in saline or buffers for neutralizing internal acid conditions or may be administered without any carriers.
Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.
Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. Microspheres formulated for oral administration may also be used. Such microspheres have been well defined in the art. All formulations for oral administration should be in dosages suitable for such administration.
For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.
The compounds may be administered by inhalation to pulmonary tract, especially the bronchi and more particularly into the alveoli of the deep lung, using standard inhalation devices. The compounds may be delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit may be determined by providing a valve to deliver a metered amount. An inhalation apparatus may be used to deliver the compounds to a subject. An inhalation apparatus, as used herein, is any device for administering an aerosol, such as dry powdered form of the compounds. This type of equipment is well known in the art and has been described in detail, such as that description found in Remington: The Science and Practice of Pharmacy, 19th Edition, 1995, Mac Publishing Company, Easton, Pa., pages 1676-1692. Many U.S. patents also describe inhalation devices, such as U.S. Pat. No. 6,116,237.
“Powder” as used herein refers to a composition that consists of finely dispersed solid particles. Preferably the compounds are relatively free flowing and capable of being dispersed in an inhalation device and subsequently inhaled by a subject so that the compounds reach the lungs to permit penetration into the alveoli. A “dry powder” refers to a powder composition that has a moisture content such that the particles are readily dispersible in an inhalation device to form an aerosol. The moisture content is generally below about 10% by weight (% w) water, and in some embodiments is below about 5% w and preferably less than about 3% w. The powder may be formulated with polymers or optionally may be formulated with other materials such as liposomes, albumin and/or other carriers.
Aerosol dosage and delivery systems may be selected for a particular therapeutic application by one of skill in the art, such as described, for example in Gonda, I. “Aerosols for delivery of therapeutic and diagnostic agents to the respiratory tract,” in Critical Reviews in Therapeutic Drug Carrier Systems, 6:273-313 (1990), and in Moren, “Aerosol dosage forms and formulations,” in Aerosols in Medicine. Principles, Diagnosis and Therapy, Moren, et al., Eds., Esevier, Amsterdam, 1985.
The compounds, when it is desirable to deliver them systemically, may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.
Pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.
Alternatively, the active compounds may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
The compounds may also be formulated in rectal or vaginal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
The pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.
Suitable liquid or solid pharmaceutical preparation forms are, for example, aqueous or saline solutions for inhalation, microencapsulated, encochleated, coated onto microscopic gold particles, contained in liposomes, nebulized, aerosols, pellets for implantation into the skin, or dried onto a sharp object to be scratched into the skin. The pharmaceutical compositions also include granules, powders, tablets, coated tablets, (micro)capsules, suppositories, syrups, emulsions, suspensions, creams, drops or preparations with protracted release of active compounds, in whose preparation excipients and additives and/or auxiliaries such as disintegrants, binders, coating agents, swelling agents, lubricants, flavorings, sweeteners or solubilizers are customarily used as described above. The pharmaceutical compositions are suitable for use in a variety of drug delivery systems. For a brief review of methods for drug delivery, see Langer, Science 249:1527-1533, 1990, which is incorporated herein by reference.
The CpG immunostimulatory oligonucleotides and optionally other therapeutics and/or antigens may be administered per se (neat) or in the form of a pharmaceutically acceptable salt. When used in medicine the salts should be pharmaceutically acceptable, but non-pharmaceutically acceptable salts may conveniently be used to prepare pharmaceutically acceptable salts thereof. Such salts include, but are not limited to, those prepared from the following acids: hydrochloric, hydrobromic, sulphuric, nitric, phosphoric, maleic, acetic, salicylic, p-toluene sulphonic, tartaric, citric, methane sulphonic, formic, malonic, succinic, naphthalene-2-sulphonic, and benzene sulphonic. Also, such salts can be prepared as alkaline metal or alkaline earth salts, such as sodium, potassium or calcium salts of the carboxylic acid group.
Suitable buffering agents include: acetic acid and a salt (1-2% w/v); citric acid and a salt (1-3% w/v); boric acid and a salt (0.5-2.5% w/v); and phosphoric acid and a salt (0.8-2% w/v). Suitable preservatives include benzalkonium chloride (0.003-0.03% w/v); chlorobutanol (0.3-0.9% w/v); parabens (0.01-0.25% w/v) and thimerosal (0.004-0.02% w/v).
The pharmaceutical compositions of the invention contain an effective amount of a CpG immunostimulatory oligonucleotide and optionally antigens and/or other therapeutic agents optionally included in a pharmaceutically-acceptable carrier. The term pharmaceutically-acceptable carrier means one or more compatible solid or liquid filler, diluents or encapsulating substances which are suitable for administration to a human or other vertebrate animal. The term carrier denotes an organic or inorganic ingredient, natural or synthetic, with which the active ingredient is combined to facilitate the application. The components of the pharmaceutical compositions also are capable of being commingled with the compounds of the present invention, and with each other, in a manner such that there is no interaction which would substantially impair the desired pharmaceutical efficiency.
The present invention is further illustrated by the following Examples, which in no way should be construed as further limiting. The entire contents of all of the references (including literature references, issued patents, published patent applications, and co-pending patent applications) cited throughout this application are hereby expressly incorporated by reference.
EXAMPLES
Materials and Methods:
Oligodeoxynucleotides All ODNs were provided by Coley Pharmaceutical GmbH (Langenfeld, Germany). ODNs were diluted in phosphate-buffered saline (Sigma, Germany), and stored at −20° C. All dilutions were carried out using pyrogen-free reagents. The ODNs used in the studies described below are shown in Table 1.







TABLE 1





Sequences of ODNs shown in the drawings.

















T*T*T*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 1)



 


T*T


 


T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T
(SEQ. ID NO.: 2)


 


*T


 


T*G*A*C*T*G*T*G*A*A*C*G*T*T*C*G*
(SEQ. ID NO.: 3)


 


A*G*A*T*G*A


 


T*C*G*T*G*A*C*T*G*T*G*A*A*C*G*T*
(SEQ. ID NO.: 4)


 


T*C*G*A*G*A*T*G*A


 


T*C*G*C*T*G*T*G*A*A*C*G*T*T*C*G*
(SEQ. ID NO.: 5)


 


A*G*A*T*G*A


 


T*C*C*A*G*G*A*C*T*T*C*T*C*T*C*A*
(SEQ. ID NO.: 6)


 


G*G*T*T


 


T*C*G*T*C*C*A*G*G*A*C*T*T*C*T*C*
(SEQ. ID NO.: 7)


 


T*C*A*G*G*T*T


 


T*T*T*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 8)


 


T


 


T*C*G*A*A*A*A*A*A*A*A*A*A*A*A*A*
(SEQ. ID NO.: 9)


 


A


 


T*G*C*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 10)


 


T


 


T*T*T*T*T*T*T*T*T*T*T*T*T*T*T*C*
(SEQ. ID NO.: 11)


 


G


 


C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 12)


 


T


 


T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 13)


 


T*T*T*T*T


 


A*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 14)


 


T


 


C*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 15)


 


T


 


G*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 16)


 


T


 


T*T*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 17)


 


T


 


T*G*T*C*G*T*T*G*T*C*G*T*T*G*T*C*
(SEQ. ID NO.: 18)


 


G*T*T*G*T*C*G*T*T


 


T*C*G*T*C*G*T*T*G*T*C*G*T*T*G*T*
(SEQ. ID NO.: 19)


 


C*G*T*T*G*T*C*G*T*T


 


T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*
(SEQ. ID NO.: 20)


 


C*G*T*T


 


T*C*G*A*T*G*A*C*G*T*T*C*C*T*G*A*
(SEQ. ID NO.: 21)


 


C*G*T*T


 


T*C*G*T*C*G*T*C*C*A*G*G*A*C*T*T*
(SEQ. ID NO.: 22)


 


C*T*C*T*C*A*G*G*T*T


 


T*C*G*T*C*G*C*T*G*T*G*A*A*C*G*T*
(SEQ. ID NO.: 23)


 


T*C*G*A*G*A*T*G*A


 


T*C*G*T*G*A*C*T*G*T*G*A*A*C*G*T*
(SEQ. ID NO.: 24)


 


T*C*G*A*G*A*T*G*A


 


T*C_G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 25)


 


T


 


T*C*G*T*C*G*T*T*T*T*G*T*C*G*T*T*
(SEQ. ID NO.: 26)


 


T*T*G*T*C*G*T*T


 


T*T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 27)


 


T


 


T*T*T*C*G*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 28)


 


T


 


T*T*T*T*C*G*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 29)


 


T


 


T*T*T*T*T*C*G*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 30)


 


T


 


T*C*C*A*G*G*A*C*T*T*C*T*C*T*C*A*
(SEQ. ID NO.: 31)


 


G*G*T*T


 


T*C*G*T*T*T*T*T*T*T*T*T*T
(SEQ. ID NO.: 32)


 


T*C_G*T*C_G*T*T*T*T_G*T*C_G*T*T
(SEQ. ID NO.: 33)


 


T*T*C_G*T*C_G*T*T*T*C_G*T*C_G*T*
(SEQ. ID NO.: 34)


 


T


 


T*T*C_G*T*C_G*T*T*T*T_G*T*C_G*T*
(SEQ. ID NO.: 35)


 


T


 


T*T*C*G*T*C*G*T*T*T*C*G*T*C*G*T*
(SEQ. ID NO.: 36)


 


T


 


T*T*T*C_G*T*C_G*T*T*T*C_G*T*C_G*T
(SEQ. ID NO.: 37)


 


*T


 


T*T*G_C*T*C_G*T*T*T*C_G*T*C_G*T*T
(SEQ. ID NO.: 38)


 


T*T*G_C*T*G_C*T*T*T*C_G*T*C_G*T*T
(SEQ. ID NO.: 39)


 


T*T*G_C*T*G_C*T*T*T*G_C*T*G_C*T*T
(SEQ. ID NO.: 40)


 


T*C*G*A*A*A*A*A*A*A*A*A*A*T*A*A*
(SEQ. ID NO.: 41)


 


A


 


T*C*G*A*A*A*A*A*A*A*A*A*T*T*A*A*
(SEQ. ID NO.: 42)


 


A


 


T*C*G*A*A*A*A*A*A*A*T*T*T*T*A*A*
(SEQ. ID NO.: 43)


 


A


 


T*C*G*A*A*A*A*A*T*T*T*T*T*T*A*A*
(SEQ. ID NO.: 44)


 


A


 


T*C*G*A*A*A*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 45)


 


A


 


T*C*G*T*A*A*A*A*A*A*A*A*A*A*A*A*
(SEQ. ID NO.: 46)


 


A


 


T*C*G*T*T*T*A*A*A*A*A*A*A*A*A*A*
(SEQ. ID NO.: 47)


 


A


 


T*C*G*U*U*U*U*U*U*U*U*U*U*U*U*U*
(SEQ. ID NO.: 48)


 


U


 


U*U*U*U*U*U*U*U*U*U*U*U*U*U*U*U*
(SEQ. ID NO.: 49)


 


U


 


T*C*G*A*G*G*A*C*T*T*C*T*C*T*C*A*
(SEQ. ID NO.: 50)


 


G*G*T*T


 


T*C*G*C*C*C*C*C*C*C*C*C*C*C*C*C*
(SEQ. ID NO.: 51)


 


C


 


T*C*G*T*C*G*A*G*C*G*T*G*C*G*C*C*
(SEQ ID NO. 52)


 


A*T


 


T*C*G*C*C*C*A*G*C*G*T*G*C*G*C*C*
(SEQ ID NO. 53)


 


A*T


 


U*C*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 54)


 


T


 


T*C*U*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 55)


 


T


 


T*U*G*T*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ. ID NO.: 56)


 


T


 


T*C*G*T*T*T*T*T*T*T*T*T*T*T*T
(SEQ ID NO. 57)


 


T*T*C*G*T*T*T*T*T*T*T*T*T*T*T*T*
(SEQ ID NO. 58)


 


T


 


T*T*T*T*T*T*T*T*T*C*G*T*T*T*T*T*
(SEQ ID NO. 59)


 


T


 


T*C*T*C*C*C*A*G*C*G*T*G*C*G*C*C*
(SEQ ID NO. 60)


 


A*T





*: Phosphorothioate linkage;


_: Phosphodiester linkage.






Cell purification Peripheral blood buffy coat preparations from healthy male and female human donors were obtained from the German Red Cross (Rathingen, Germany) or from the Blood Bank of the University of Düsseldorf (Germany) and from these, PBMC were purified by centrifugation over Ficoll-Hypaque (Sigma). The purified PBMC were either used fresh (for most assays) or were suspended in freezing medium and stored at −70° C. When required, aliquots of these cells were thawed, washed and resuspended in RPMI 1640 culture medium supplemented with 5% (v/v) heat inactivated human AB serum (BioWhittaker, Belgium) or 10% (v/v) heat inactivated FCS, 1.5 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin (all from Sigma).
Cytokine detection Thawed or fresh PBMC were resuspended at a concentration of 3×106/ml to 5×106/ml and added to plates which had previously received nothing or ODN in a variety of concentrations. The cells were cultured in a humidified incubator at 37° C. Culture supernatants were collected after the indicated time points. If not used immediately, supernatants were frozen at −20° C. until required. Amounts of cytokines in the supernatants were assessed using commercially available ELISA Kits or in-house ELISA developed using commercially available antibodies (e.g. from Becton Dickinson, Germany).
Example 1
5′-TCG Enhances Immunostimulatory Activity of Non-CpG or CpG ODNs (IL-10)
Human PBMC of two representative donors were incubated for 48 h with the indicated ODNs (FIG. 1). Supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods. The activity of non-CpG ODNs such as poly T ODNs or another non-CpG ODN, SEQ. ID NO.: 6, were strongly enhanced by adding a TCG trinucleotide to the 5′ end. CpG ODNs lacking a 5′-TCG such as SEQ. ID NO.: 3, (Magone et al., Eur. J. Immunol. 2000; 30: 1841-1850) could also be modified to exhibit higher potency and/or efficacy with the addition of a 5′TCG.
Example 2
TCG Enhances Immunostimulatory Activity of Non-CpG or CpG ODNs (IFN-α)
Human PBMC of two representative donors were incubated for 48 h with the indicated ODNs (FIG. 2). Supernatants were harvested and IFN-α measured by ELISA as described in Materials and Methods. The effects of a 5′-TCG modification for the same ODNs as shown in FIG. 1 are demonstrated in this IFN-α assay.
Example 3
Enhancement of the Immune Response is Dependent on the CpG Dinucleotide
Human PBMC of two representative donors were incubated for 48 h with the indicated ODNs (FIG. 3). Supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods. Shown are the effect of a 5′-TCG, SEQ. ID NO.: 2, and a poly T sequence, SEQ. ID NO.: 8. Although a 5′-TGC, SEQ. ID NO.: 10, may have some minimal effect, the 5′-TCG modification clearly resulted in a much stronger potentiation of cytokine secretion. A 5′-TCG modification of a 17mer poly A ODN did not appear to have an effect, SEQ. ID NO.: 9. Similar results were obtained for interferon secretion. In contrast to the 5′-TCG plus poly A ODN a 5′-TCG ODN in a poly uracil context lead to enhancement of IL-10 secretion (Shown in FIG. 6).
Example 4
Shifting the CpG Dinucleotide from the 5′ to the 3′ End of an ODN Results in Graded Loss of Immunostimulatory Capability
Human PBMC of two representative donors were incubated for 48 h with the indicated ODNs (FIG. 4). Supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods. The CpG dinucleotide at the 5′ end of an ODN resulted in enhanced IL-10 secretion, SEQ. ID NO.: 2. Shifting the CpG to the 3′ end, SEQ. ID NO.: 11, of a poly T ODN resulted in strongly reduced cytokine secretion. A 5′-CG, SEQ. ID NO.: 12, had also a potentiating effect although a 5′-TCG was more efficient in enhancing the cytokine response. Similar results were obtained for interferon secretion.
Example 5
The Length of an ODN has an Effect on Stimulatory Activity in Addition to a 5′-TCG
Human PBMC of two representative donors were incubated for 48 h with the indicated ODNs (FIG. 5). Supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods. The data demonstrate that the length of a CpG ODN plays a role in the stimulatory activity in addition to the 5′-TCG ODNs. A 21mer is more potent and efficient than a 17mer which is more potent than a 15mer or a 13mer.
Example 6
A 5′-TCG is the Most Stimulatory 5′ Modification, but Other Modifications Also Lead to Enhanced Immunostimulation
Human PBMC of three representative donors were incubated for 48 h with the indicated ODNs (FIG. 6). Supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods. A 5′-TCG is clearly the most potent 5′ modification as demonstrated in the above experiment. Nevertheless, other 5′ modifications were also able to enhance the stimulatory capability of poly T ODNs on human cells. Surprisingly, a 5′-TC alone was able to enhance cytokine secretion. Other 5′ trinucleotides such as ACG, CCG and GCG lead also to enhanced IL-10 secretion although the 5′-TCG showed the strongest effects. Even a 5′-TTG was shown be more stimulatory than a purely poly T ODN. In addition, specific modifications of the sequence 3′ to the 5′-TCG retained immunostimulation. In contrast to the 5′-TCG plus poly A ODN (FIG. 3) a 5′-TCG ODN in a poly uracil context lead to enhancement of IL-10 secretion.
Example 7
The 5′-TCG Modification Enhances the Stimulatory Capability of CpG ODNs as Shown by Different Cellular Effects
a. Human PBMC of three representative donors were incubated for 48 h with the indicated ODNs ands supernatants were harvested and IL-10 measured by ELISA as described in Materials and Methods (FIG. 7A).
b. Human PBMC of three representative donors were incubated for 48 h with the indicated ODNs and supernatants were harvested and IFN-α measured by ELISA as described in Materials and Methods (FIG. 7B).
c. Human PBMC of two representative donors were incubated for 20 h with the indicated ODNs and supernatants were harvested and IL-6 measured by ELISA as described in Materials and Methods (FIG. 7C).
FIG. 7A to 7C demonstrate that the 5′-TCG is able to enhance the stimulatory capacity of an ODN in a variety of assays. The parent CpG ODN SEQ. ID NO.: 18 does not have a CpG dinucleotide directly at the 5′ end. Modifying the sequence with a 5′-TCG, SEQ. ID NO.: 19, also enhanced the activity of the CpG ODN.
Example 8
IL-10 Secretion Induced by ODN with 5′-TCG
Human PBMC were incubated with increasing concentrations of the indicated ODN's for 48 h. Supernatant was harvested and IL-10 measured by ELISA as described in Materials and Methods. Shown is the result for three individual donors. This experiment was a dose response study that investigated the contribution of thymidines 3′ of the 5′-TCG trinucleotide with ODN concentrations up to 1 μM. As shown in FIG. 8, at 1 μM low stimulation of cytokine secretion (IL-10) can be observed with SEQ. ID NO.: 9 (5′-TCG plus poly A).
Example 9
IL-10 Secretion Induced by ODN with 5′-TCG and Increasing Numbers of Thymidines
Human PBMC were incubated with the indicated ODN for 48 h. Supernatant was harvested and IL-10 measured by ELISA as described in Materials and Methods. Shown is the result for three individual donors. To investigate whether the addition of thymidines to the 3′ tail of ODN's would increase in vitro cytokine production, the ODN's were modified by exchanging increasing numbers of adenosines to thymidines. The addition of only one thymidine to the poly A tail led to an increase of immunostimulation as can be observed with SEQ. ID NO.: 41 (FIG. 9). Adding more thymidines led to further increase of IL-10 production, dependent on the number of thymidines. A 5′-TCG was sufficient to enhance immunostimulation by phosphorothioate ODN independent of the nucleotide sequence. Nevertheless, an increasing number of pyrimidines further contributed to this stimulation. Only two to four thymidines 3′ of the 5′-TCG (here: about at least 20% thymidines) were sufficient to lead to a significant increase of cytokine secretion (SEQ. ID NO.: 42 and SEQ. ID NO.: 43).
Example 10
ODN with a 5′-TCG are the Most Potent and Efficient ODN to Induce a Strong Th1-Mediated Immune Response
Human PBMC of three representative donors were incubated for 48 h with the indicated ODN concentrations (FIGS. 10A and 10B). Supernatants were harvested and IL-10 and IFN-α measured by ELISA as described in Materials and Methods. Shown is the Mean±SEM. The 5′-TCG led to the most potent and efficient immune stimulation of all ODN tested (in terms of the B cell related cytokine IL-10). Nevertheless, when the potential of ODN's with different 5′ ends to induce the Th1 related cytokine IFN-α was measured, it was observed that only the 5′-TCG supported strong secretion of this cytokine (FIG. 10B).
Example 11
The Position of CpG Dinucleotides in Immune Stimulatory ODN Determines the Strength of type I IFN Secretion
Human PBMC of three representative donors were incubated for 48 h with the indicated ODN concentrations. Supernatants were harvested and IFN-α measured by ELISA as described in Materials and Methods. Shown is the Mean. Shifting the CpG dinucleotide (essential for efficient immune stimulation) from the 5′ to the 3′ end led to a graded loss of immune stimulation (measured as secretion of the B cell related cytokine IL-10). FIG. 11 demonstrates that the position of the CpG also strongly influences the strength of type I IFN secretion. Surprisingly, shifting the CpG only one to three positions to the 3′ end led to strongly enhanced IFN-α secretion especially with ODN SEQ. ID NO.: 27 and SEQ. ID NO.: 28. Shifting further to the 3′ end led to strong decrease of IFN-α secretion below the level of SEQ. ID NO.: 2 (5′-TCG).
Example 12
Type I IFN Secretion Induced by Short 5′-TCG ODN
Human PBMC of three representative donors were incubated for 48 h with the indicated ODN concentrations. Supernatants were harvested and IFN-α measured by ELISA as described in Materials and Methods. Shown is the Mean±SEM. Previous findings have shown decrease of immune stimulation (measured as IL-10 secretion) upon shortening of the ODN's length. Nevertheless, when the secretion of IFN-α by shortened ODN's (e.g. 13mer SEQ. ID NO.: 32 with 5′-TCG) was measured, surprisingly a strongly increased IFN-α secretion compared to the 17mer SEQ. ID NO.: 2 was observed (FIG. 12).
Example 13
In Vitro Immune Stimulation by a Panel of Newly Generated CpG ODN According to the Observations Described Herein
The important observations that were described in the previous examples were:


    
    
        a. A 5′ TCG supports efficient and potent IFN-α (a Th1 related cytokine) as well as IL-10 secretion (a B cell related cytokine);
        b. Shifting the CpG dinucleotide from the 5′ to the 3′ end led first to an increase of type I IFN secretion and further 3′ shifts led to a decrease (B cell activation was only decreased or only slightly changed by CpG shifts);
        c. Shortening an ODN with a 5′-TCG led to a strong increase in the potential to induce IFN-α (in contrast to other effects, e.g. secretion of IL-10);
    
    


These observations were combined and a panel of short CpG ODN's was created that were tested for their potential to induce the secretion of IFN-α as well as to activate B cells. Human PBMC of three representative donors were incubated for 48 h with the indicated ODN concentrations. Supernatants were harvested and IL-10 and IFN-α measured by ELISA as described in Materials and Methods. Shown is the Mean±SEM. As demonstrated in FIG. 13A ODN's were generated with lengths below 20 nucleotides that induced more efficient IFN-α secretion than a typical 24mer B-Class ODN, SEQ. ID NO.: 26. The difference between ODN SEQ. ID NO.: 36 (PS) and SEQ. ID NO.: 35 (semi-soft) in FIG. 13A indicated a shift of the bell-shaped curve to lower ODN concentrations (down-turn of the activation curve can be observed at lower ODN concentrations with SEQ. ID NO.: 35). In addition, loss of single to all CpG dinucleotides as in SEQ. ID NO.: 38 to SEQ. ID NO.: 40 led to a decrease of cytokine secretion, confirming that the observed effects were CpG-dependent. FIG. 13B (and FIG. 14) demonstrate that such short ODN were perfectly able to induce the activation of B cells (measured as CD80 up-regulation on CD19-positive B cells as well as secretion of the cytokine IL-10 produced by B cells).
Example 14
Short CpG ODN are Perfectly able to Induce Efficient B Cell Stimulation
Human PBMC of three representative donors were incubated for 24 h with the indicated ODN concentrations and cells harvested and stained for CD19, CD14 and CD80. Expression of CD80 on CD19-positive B cells was measured by flow cytometry as described. FIG. 14 demonstrates that such short ODN were perfectly able to induce the activation of B cells (measured as CD80 up-regulation on CD19-positive B cells as well as secretion of the cytokine IL-10 produced by B cells).
Example 15
A Phosphodiester Linkage Between the C and G of the 5′ CpG Dinucleotide Results in Enhancement of Potency of Immune Stimulation
Human PBMC of three representative donors were incubated with the indicated ODN concentrations for 48 h. Supernatants were harvested and IL-10 measured by ELISA as described above. The introduction of a phosphodiester linkage between the CpG dinucleotide in an ODN with a 5′-TCG, SEQ. ID NO.: 25, led to a shift of the IL-10 secretion to lower ODN concentrations compared to an ODN with an unmodified 5′-TCG (SEQ. ID NO.: 2). The data is shown in FIG. 15. A similar result was obtained for IFN-alpha secretion.
Example 16
Modifications of the T Preceeding the 5′-CG are Allowed
Human PBMC of three representative donors were incubated with the indicated ODN concentrations for 48 h. Supernatants were harvested and IL-10 measured by ELISA as described above. The result shown in FIG. 16 demonstrate that:

    
    
        1. an ODN with a 5′-UCG (SEQ. ID NO. 54) induced similar strong cytokine secretion as an ODN with a 5′-TCG (SEQ. ID NO.: 2). Both ODN were superior to a pure poly T ODN (SEQ. ID NO.: 8). This result suggests that a variety of chemical modified nucleotides 5′ to the CpG are allowed to induce an enhanced immune stimulation.
        2. ODN with a 5′-TCU (SEQ. ID NO. 55) or 5′-TUG (SEQ. ID NO. 56) also demonstrated enhanced cytokine secretion when compared to a poly T ODN (SEQ. ID NO.: 8). Nevertheless, a 5′-TCG was superior to these two modifications and the 5′-TCU induced more efficient IL-10 secretion than the 5′-TUG. These results suggest that a variety of chemical modifications at the CpG dinucleotide are allowed to induce enhanced immune stimulation.
    
    


The foregoing written specification is considered to be sufficient to enable one skilled in the art to practice the invention. Various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and fall within the scope of the appended claims. The advantages and objects of the invention are not necessarily encompassed by each embodiment of the invention.

## Claims

1. An isolated oligonucleotide comprising:
5′TCGX1X2N13′
wherein X1 is any nucleotide, X2 is A, T, or C when X1 is C or A, X2 is A or G when X1 is T, X2 is any nucleotide when X1 is G, N1 is 2-95 nucleotides, wherein the CG dinucleotide is an unmethylated CG dinucleotide, wherein 5′ designates the 5′ end of the oligonucleotide and 3′ designates the 3′ end of the oligonucleotide, and wherein N1 does not include an unmethylated CG motif, wherein the oligonucleotide is 13-100 nucleotides in length.

2. The oligonucleotide of claim 1, wherein the oligonucleotide includes at least 1 modified internucleotide linkage.

3. The oligonucleotide of claim 1, wherein the oligonucleotide includes at least 50% modified internucleotide linkage.

4. The oligonucleotide of claim 1, wherein all internucleotide linkages of the oligonucleotide are modified.

5. The oligonucleotide of claim 2, wherein the stabilized internucleotide linkage is a phosphorothioate linkage.

6. The oligonucleotide of claim 1, wherein N1 is N2N3 and wherein N2 is 8-40 nucleotides and N3 is 2-5 pyrimidines.

7. The oligonucleotide of claim 6, wherein N3 is TTTTT.

8. The oligonucleotide of claim 6, wherein N3 is TT.

9. The oligonucleotide of claim 1, wherein N1 is at least 50% pyrimidine.

10. The oligonucleotide of claim 1 , wherein N1 is at least 80% pyrimidine.

11. The oligonucleotide of claim 1, wherein N1 is free of Poly-A and Poly-G sequences.

12. The oligonucleotide of claim 1, wherein N1 is TN2 and wherein N2 is 8-94 nucleotides.

13. The oligonucleotide of claim 1, wherein the oligonucleotide has a 3′-3′ linkage with one or two accessible 5′ ends.

14. The oligonucleotide of claim 13, wherein the oligonucleotide has two accessible 5′ ends, each of which are 5′TCG.

15. A method for inducing cytokine production, comprising:
administering to a subject an oligonucleotide of claim 1 in an effective amount to induce a cytokine selected from the group consisting of, IFN-α and IL-10.