Lassa Fever

National Center for Emerging and Zoonotic Infectious Diseases

Division of High-Consequence Pathogens and Pathology (DHCPP)

Lassa fever is an acute viral illness that occurs in west Africa. The illness was discovered in 1969 when two missionary nurses died 

in Nigeria. The virus is named after the town in Nigeria where the first cases occurred. The virus, a member of the virus family 

Arenaviridae, is a single-stranded RNA virus and is zoonotic, or animal-borne.

Lassa fever is endemic in parts of west Africa including Sierra Leone, Liberia, Guinea and Nigeria; however, other neighboring 

countries are also at risk, as the animal vector for Lassa virus, the "multimammate rat" (Mastomys natalensis) is distributed 

throughout the region. In 2009, the first case from Mali was reported in a traveler living in southern Mali; Ghana reported its first 

cases in late 2011. Isolated cases have also been reported in Côte d’Ivoire and Burkina Faso and there is serologic evidence of 

Lassa virus infection in Togo and Benin.

The number of Lassa virus infections per year in west Africa is estimated at 100,000 to 300,000, with approximately 5,000 deaths. 

Unfortunately, such estimates are crude, because surveillance for cases of the disease is not uniformly performed. In some areas of 

Sierra Leone and Liberia, it is known that 10%-16% of people admitted to hospitals every year have Lassa fever, which indicates 

the serious impact of the disease on the population of this region. 

Transmission

The reservoir, or host, of Lassa virus is a rodent known as the "multimammate rat" (Mastomys natalensis). Once infected, this 

rodent is able to excrete virus in urine for an extended time period, maybe for the rest of its life. Mastomys rodents breed frequently, 

produce large numbers of offspring, and are numerous in the savannas and forests of west, central, and east Africa. In addition, 

Mastomys readily colonize human homes and areas where food is stored. All of these factors contribute to the relatively efficient 

spread of Lassa virus from infected rodents to humans.

Transmission of Lassa virus to humans occurs most commonly through ingestion or inhalation. Mastomys rodents shed the virus in 

urine and droppings and direct contact with these materials, through touching soiled objects, eating contaminated food, or exposure 

to open cuts or sores, can lead to infection.

Because Mastomys rodents often live in and around homes and scavenge on leftover human food items or poorly stored food, 

direct contact transmission is common. Mastomys rodents are sometimes consumed as a food source and infection may occur 

when rodents are caught and prepared. Contact with the virus may also occur when a person inhales tiny particles in the air 

contaminated with infected rodent excretions. This aerosol or airborne transmission may occur during cleaning activities, such as 

sweeping.

Direct contact with infected rodents is not the only way in which people are infected; person-to-person transmission may occur after 

exposure to virus in the blood, tissue, secretions, or excretions of a Lassa virus-infected individual. Casual contact (including skinto-skin contact without exchange of body fluids) does not spread Lassa virus. Person-to-person transmission is common in health 

care settings (called nosocomial transmission) where proper personal protective equipment (PPE) is not available or not used.

Lassa virus may be spread in contaminated medical equipment, such as reused needles. 

Signs and Symptoms

Signs and symptoms of Lassa fever typically occur 1-3 weeks after the patient comes into contact with the virus. For the majority of 

Lassa fever virus infections (approximately 80%), symptoms are mild and are undiagnosed. Mild symptoms include slight fever, 

general malaise and weakness, and headache. In 20% of infected individuals, however, disease may progress to more serious 

symptoms including hemorrhaging (in gums, eyes, or nose, as examples), respiratory distress, repeated vomiting, facial swelling, 

pain in the chest, back, and abdomen, and shock. Neurological problems have also been described, including hearing loss, tremors, 

and encephalitis. Death may occur within two weeks after symptom onset due to multi-organ failure.

The most common complication of Lassa fever is deafness. Various degrees of deafness occur in approximately one-third of 

infections, and in many cases hearing loss is permanent. As far as is known, severity of the disease does not affect this 

complication: deafness may develop in mild as well as in severe cases.

Approximately 15%-20% of patients hospitalized for Lassa fever die from the illness. However, only 1% of all Lassa virus infections 

result in death. The death rates for women in the third trimester of pregnancy are particularly high. Spontaneous abortion is a 

serious complication of infection with an estimated 95% mortality in fetuses of infected pregnant mothers.

Because the symptoms of Lassa fever are so varied and nonspecific, clinical diagnosis is often difficult. Lassa fever is also 

associated with occasional epidemics, during which the case-fatality rate can reach 50% in hospitalized patients. 

Risk of Exposure

Individuals at greatest risk of Lassa virus infection are those who live in or visit endemic regions, including Sierra Leone, Liberia, 

Guinea, and Nigeria and have exposure to the multimammate rat. Risk of exposure may also exist in other west African countries 

where Mastomys rodents exist. Hospital staff are not at great risk for infection as long as protective measures and proper 

sterilization methods are used. 
Diagnosis

Lassa fever is most often diagnosed by using enzyme-linked immunosorbent serologic assays (ELISA), which detect IgM and IgG 

antibodies as well as Lassa antigen. Reverse transcription-polymerase chain reaction (RT-PCR) can be used in the early stage of 

disease. The virus itself may be cultured in 7 to 10 days, but this procedure should only be done in a high containment laboratory 

with good laboratory practices. Immunohistochemistry, performed on formalin-fixed tissue specimens, can be used to make a postmortem diagnosis. 

Treatment

Ribavirin, an antiviral drug, has been used with success in Lassa fever patients. It has been shown to be most effective when given 

early in the course of the illness. Patients should also receive supportive care consisting of maintenance of appropriate fluid and 

electrolyte balance, oxygenation and blood pressure, as well as treatment of any other complicating infections. 

Prevention

Primary transmission of the Lassa virus from its host to humans can be prevented by avoiding contact with Mastomys rodents, 

especially in the geographic regions where outbreaks occur. Putting food away in rodent-proof containers and keeping the home 

clean help to discourage rodents from entering homes. Using these rodents as a food source is not recommended. Trapping in and 

around homes can help reduce rodent populations; however, the wide distribution of Mastomys in Africa makes complete control of 

this rodent reservoir impractical.

When caring for patients with Lassa fever, further transmission of the disease through person-to-person contact or nosocomial 

routes can be avoided by taking preventive precautions against contact with patient secretions (called VHF isolation precautions or 

barrier nursing methods). Such precautions include wearing protective clothing, such as masks, gloves, gowns, and goggles; using 

infection control measures, such as complete equipment sterilization; and isolating infected patients from contact with unprotected 

persons until the disease has run its course.

Further, educating people in high-risk areas about ways to decrease rodent populations in their homes will aid in the control and 

prevention of Lassa fever. Other challenges include developing more rapid diagnostic tests and increasing the availability of the only 

known drug treatment, ribavirin. Research is presently under way to develop a vaccine for Lassa fever.

References

• Amorosa V, MacNeil A, McConnell R, et al. Imported Lassa Fever, Pennsylvania, USA, 2010. Emerging Infectious 

Diseases. 2010;16(10):1598-600.

• Asogun DA, Adomeh DI, Ehimuan J, et al. Molecular diagnostics for Lassa Fever at Irrua specialist teaching hospital, 

Nigeria: lessons learnt from two years of laboratory operation. PLoS Neglected Tropical Diseases. 2012;6(9):e1839. 

• Baize S, Marianneau P, Georges-Courbot M-C, et al. Recent advances in vaccines against viral haemorrhagic fevers. 

Current Opinion in Infectious Diseases. 2001;14(5):513-8.

• Bausch DG, Demby AH, Coulibaly M, et al. Lassa fever in Guinea, West Africa. I. Epidemiology of human disease and

clinical observations. Vector Borne and Zoonotic Diseases. 2001;1:269-82. 

• Crowcroft N. Management of Lassa Fever in European countries. Euro Surveillance. 2002;7(3):50-2.

• Cummins D, Bennett D, Fisher-Hoch SP, et al. Lassa fever encephalopathy: clinical and laboratory findings. Journal of 

Tropical Medicine and Hygiene. 1992;95:197-201. 

• Cummins D, McCormick JB, Bennet D, et al. Acute sensorineural deafness in Lassa fever. Journal of the American 

Medical Association. 1991;264(16):2093-6.

• Demby AH, Inapougui A, Kargbo K, et al. Lassa fever in Guinea. II: Distribution and prevalence of Lassa virus infection in 

small mammals. Vector Borne and Zoonotic Diseases. 2001;1:283-97. 

• Fichet-Calvet E, Rogers DJ. Risk maps of Lassa Fever in West Africa. PLoS Neglected Tropical Diseases. 2009;3(3):e388.

• Frame JD. The story of Lassa fever. Part I: Discovering the disease. New York State Journal of Medicine. 1992;92(5):199-

202.

• Frame JD, Baldwin JM, Gocke DJ, et al. Lassa fever, a new virus disease of man from West Africa. I. Clinical description 

and pathological findings. American Journal of Tropical Medicine and Hygiene. 1970;19:670-9.

• Gunther S, Lenz O. Lassa virus. Critical Reviews in Clinical Laboratory Sciences. 2004;41(4):339-90.

• Haas WH, Breuer T, Pfaff G, et al. Imported Lassa fever in Germany: surveillance and management of contact persons. 

Clinical Infectious Diseases. 2003;36(10):1254-8.

• Hensley LE, Smith MA, Geisbert JB, et al. Pathogenesis of Lassa fever in cynomolgus macaques. Virology. 2011;8:205.

• Johnson KM, McCormick JB, Webb PA, et al. Clinical virology of Lassa fever in hospitalized patients. Journal of Infectious 

Diseases. 1987;155(3):456-64.

• Lecompte E, Fichet-Calvet E, Daffis S, et al. Mastomys natalensis and Lassa fever, West Africa. Emerging Infectious

Diseases. 2006;12(12):1971-4.

• McCormick JB, Fisher-Hoch SP. Lassa Fever. Current Topics in Microbiology and Immunology. 2002;262:75-109.

• McCormick JB, Webb PA, Krebs JW, et al. A prospective study of the epidemiology and ecology of Lassa fever. Journal of

Infectious Diseases. 1987;155(3):437-44.
• Monath TP, Newhouse VF, Kemp GE, et al. Lassa virus isolation from Mastomys natalensis rodents during an epidemic in 

Sierra Leone. Science. 1974;185:263-5.

• Rollin PE, Nichol ST, Zaki S, Ksiazek TG. Arenaviruses and filoviruses. in: Versalovic J, Carroll KC, Funke G, Jorgensen 

JH, Landry ML, Warnock DW eds. Manual of Clinical Microbiology. 10th edition. Vol. 2. Washington, DC: ASM Press, 

2011;1514-29. 

• Speir RW, Wood OL, Liebhaber H, et al. Lassa fever, a new virus disease of man from West Africa. IV. Electron 

microscopy of Vero cell cultures infected with Lassa virus. American Journal of Tropical Medicine and Hygiene. 

1970;19:692-4.

• Troup JM, White HA, Fom AL, et al. An outbreak of Lassa fever on the Jos Plateau, Nigeria, in January-February 1970, A 

preliminary report. American Journal of Tropical Medicine and Hygiene. 1970;19:695-6. 

• World Health Organization. Update on Lassa fever in West Africa. Weekly Epidemiology Record. 2005;80(10):86-8.