APPENDIX H. Supporting Data Used for the Aquatic, Probabilistic Risk Assessment of 

Endosulfans 

I. Data Used for Derivation of Species Sensitivity Distributions 

Table H-1. The LC50 values used for the freshwater fish species sensitivity distribution in Figure 

5.5. 

Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * 

Anabas testudineus, Climbing perch 4 1.2 1.2 5326 

Anguilla anguilla, Common eel 4 41 41 11055 

Aplocheilichthys johnstonii, Tooth carp 1 2.57 2.57 15049 

Barbus conchonius, Rosy barb 2 21.36 21.36 7230 

Barbus sophore, Two spot barb, dotted barb 4 1.2 1.2 9276 

Barilius bendelisis, Cyprinid fish 4 14.71 15.79 89782 

Barilius bendelisis, Cyprinid fish 4 16.95 89782 

Bidyanus bidyanus, Silver perch 4 2.3 2.35 5850 

Bidyanus bidyanus, Silver perch 4 2.4 5850 

Channa orientalis, Smooth-breasted snakefish 4 10.55 10.59 5736 

Channa orientalis, Smooth-breasted snakefish 4 10.59 5736 

Channa orientalis, Smooth-breasted snakefish 4 10.6 5736 

Channa orientalis, Smooth-breasted snakefish 4 10.64 5736 

Channa punctata, Snake-head catfish 4 0.16 2.91 15157 

Channa punctata, Snake-head catfish 4 2.5 15157 

Channa punctata, Snake-head catfish 7 3.011 72552 

Channa punctata, Snake-head catfish 4 4.8 15157 

Channa punctata, Snake-head catfish 4 6.6 15157 

Channa punctata, Snake-head catfish 4 16 15157 

Clarias batrachus, Walking catfish 7 3.011 70.3 72552 

Clarias batrachus, Walking catfish 4 200 88885 

Clarias batrachus, Walking catfish 4 576 89006 

Cyprinus carpio, common carp 4 0.1 3.23 5850 

Cyprinus carpio, common carp 4 5.2 17179 

Cyprinus carpio, common carp 4 9.5 103230 

Cyprinus carpio, common carp 4 22 71889 

Gambusia affinis, Western mosquitofish 4 1.3 2.96 5806 

Gambusia affinis, Western mosquitofish 4 2.3 5850 

Gambusia affinis, Western mosquitofish 4 3.2 568 

Gambusia affinis, Western mosquitofish 4 8 568 

Heteropneustes fossilis, Indian catfish 4 7.4 12.94 10497 

Heteropneustes fossilis, Indian catfish 4 7.7 10497 

Heteropneustes fossilis, Indian catfish 4 7.7 10497 

Heteropneustes fossilis, Indian catfish 4 7.8 10497 

Heteropneustes fossilis, Indian catfish 4 9.1 10497 

H-1
Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * 

Heteropneustes fossilis, Indian catfish 4 9.8 10497 

Heteropneustes fossilis, Indian catfish 4 10 10497 

Heteropneustes fossilis, Indian catfish 4 10.3 10497 

Heteropneustes fossilis, Indian catfish 4 10.6 10497 

Heteropneustes fossilis, Indian catfish 4 10.7 10497 

Heteropneustes fossilis, Indian catfish 4 10.8 15179 

Heteropneustes fossilis, Indian catfish 4 12.4 10497 

Heteropneustes fossilis, Indian catfish 4 12.7 10497 

Heteropneustes fossilis, Indian catfish 4 14.1 10497 

Heteropneustes fossilis, Indian catfish 4 14.1 10497 

Heteropneustes fossilis, Indian catfish 4 14.3 10497 

Heteropneustes fossilis, Indian catfish 4 15.1 10497 

Heteropneustes fossilis, Indian catfish 4 16.9 10497 

Heteropneustes fossilis, Indian catfish 4 17 10497 

Heteropneustes fossilis, Indian catfish 4 18.1 10497 

Heteropneustes fossilis, Indian catfish 4 19.9 10497 

Heteropneustes fossilis, Indian catfish 4 20.3 10497 

Heteropneustes fossilis, Indian catfish 4 22.2 10497 

Heteropneustes fossilis, Indian catfish 4 22.8 10497 

Heteropneustes fossilis, Indian catfish 4 22.8 10497 

Ictalurus nebulosus, Channel catfish 4 1.5 1.5 submitted 

Labeo rohita, Rohu 4 0.33 1.33 468 

Labeo rohita, Rohu 4 1 468 

Labeo rohita, Rohu 4 7.1 468 

Lepomic macrochirus, Bluegill sunfish 4 1.2 1.93 submitted 

Lepomic macrochirus, Bluegill sunfish 4 1.7 submitted 

Lepomic macrochirus, Bluegill sunfish 4 2.08 submitted 

Lepomic macrochirus, Bluegill sunfish 4 3.3 submitted 

Macquaria ambigua, Yellowbelly, Golden Perch 4 0.3 0.39 5850 

Macquaria ambigua, Yellowbelly, Golden Perch 4 0.5 5850 

Macropodus cupanus, Paradise fish 4 9.61 ** 9.61 87644 

Melanotaenia duboulayi, Eastern rainbow fish 4 0.5 1.97 5850 

Melanotaenia duboulayi, Eastern rainbow fish 4 2.4 5850 

Melanotaenia duboulayi, Eastern rainbow fish 4 2.5 5850 

Melanotaenia duboulayi, Eastern rainbow fish 4 5 5850 

Monopterus albus, Swamp or White Ricefield Eel 4 0.42 0.42 100663 

Morone saxatilis, Striped bass 4 0.22 0.28 15472 

Morone saxatilis, Striped bass 4 0.23 15472 

Morone saxatilis, Striped bass 4 0.23 15472 

Morone saxatilis, Striped bass 4 0.29 15472 

Morone saxatilis, Striped bass 4 0.35 15472 

H-2
Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * 

Morone saxatilis, Striped bass 4 0.43 15472 

Nematolosa erebi, Bony bream 4 0.2 0.2 5850 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.17 0.69 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.24 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.26 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.26 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.27 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.29 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.3 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.32 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.37 submitted 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.41 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.42 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.49 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.4975 87973 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.63 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.69 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.69 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.693 87973 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.7 5850 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.75 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.79 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.8 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.83 submitted 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.89 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.1 submitted 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.21 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.3 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.34 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.372 87973 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.5 submitted 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.6 5850 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.63 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.69 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 2.43 9479 

Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 3.2769 87973 

Pimephales promelas, Fathead minnow 4 0.29 1.29 9479 

Pimephales promelas, Fathead minnow 4 0.45 9479 

Pimephales promelas, Fathead minnow 4 0.73 9479 

Pimephales promelas, Fathead minnow 4 0.75 9479 

Pimephales promelas, Fathead minnow 4 0.76 9479 

H-3
Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * 

Pimephales promelas, Fathead minnow 4 0.8 9479 

Pimephales promelas, Fathead minnow 4 0.81 9479 

Pimephales promelas, Fathead minnow 4 0.86 submitted 

Pimephales promelas, Fathead minnow 4 0.97 9479 

Pimephales promelas, Fathead minnow 4 1 9479 

Pimephales promelas, Fathead minnow 4 1.2 9479 

Pimephales promelas, Fathead minnow 4 1.2 9479 

Pimephales promelas, Fathead minnow 4 1.35 9479 

Pimephales promelas, Fathead minnow 4 1.48 9479 

Pimephales promelas, Fathead minnow 4 1.5 submitted 

Pimephales promelas, Fathead minnow 4 1.57 9479 

Pimephales promelas, Fathead minnow 4 1.67 9479 

Pimephales promelas, Fathead minnow 4 1.7 9479 

Pimephales promelas, Fathead minnow 4 1.9 9479 

Pimephales promelas, Fathead minnow 4 1.91 9479 

Pimephales promelas, Fathead minnow 4 2.1 9479 

Pimephales promelas, Fathead minnow 4 2.1 9479 

Pimephales promelas, Fathead minnow 4 2.5 9479 

Pimephales promelas, Fathead minnow 4 3.2 9479 

Pimephales promelas, Fathead minnow 4 3.2 9479 

Pimephales promelas, Fathead minnow 4 3.45 9479 

Poecilia reticulata, Guppy 4 2.7 2.7 10646 

Rasbora heteromorpha, Harlequinfish, red rasbora 4 0.2 0.2 5850 

Schilbe mystus, Butter barbel, silver barbel 1 5.11 5.11 15049 

Tilapia mossambica, Mozambique tilapia 4 6 6 9391 

Tilapia nilotica, Nile tilapia 2 0.69 0.69 20421 

Tilapia sparrmanii, Banded bream 1 7.35 7.35 15049 

* The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # labeled 

“submitted” refers to data submitted by the registrant, and can be found in Appendix E. 

** Note: in the ECOTOX database this was listed with wrong units, original publication checked. 

Table H-2. The LC50 values used for the freshwater invertebrate species sensitivity distribution in 

Figure 5.6. 

Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * 

Alonella sp., Water flea 2 0.2 0.2 786 

Atalophlebia sp., Mayfly 2 13.6 13.60 73404 

Atalophlebia sp., Mayfly 2 12.3 73404 

Atalophlebia sp., Mayfly 2 12.3 73404 

Atalophlebia sp., Mayfly 2 15.2 73404 

Atalophlebia sp., Mayfly 2 12.7 73404 

Atalophlebia sp., Mayfly 2 15.9 73404 

Barytelphusa guerini, Freshwater crab 4 17780 17780 14955 

H-4
Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * 

Bellamya dissimilis, Snail 4 1800 1800 17911 

Brachionus calyciflorus, Rotifer 1 5150 5150 5702 

Caridina laevis, Smooth Caridina 4 1.02 1.02 100785 

Cheumatopsyche sp., Caddisfly 2 1.8 0.85 20012 

Cheumatopsyche sp., Caddisfly 2 0.4 20012 

Crocothemis erythraea, Dragonfly 1 395 395 12022 

Culex fatigans, Mosquito 2 150 150 11147 

Culex pipiens, Mosquito 1 66 66 4431 

Cypria sp., Ostracod 2 0.9 0.9 786 

Daphnia carinata, Water flea 2 205 205 16513 

Daphnia longispina, Water flea 2 0.3 0.3 11147 

Daphnia magna, Water flea 2 158 451.9 9479 

Daphnia magna, Water flea 2 166 submitted 

Daphnia magna, Water flea 2 218 9479 

Daphnia magna, Water flea 2 250 9479 

Daphnia magna, Water flea 2 266 9479 

Daphnia magna, Water flea 2 271 9479 

Daphnia magna, Water flea 2 282 9479 

Daphnia magna, Water flea 2 328 9479 

Daphnia magna, Water flea 2 342 9479 

Daphnia magna, Water flea 2 372 9479 

Daphnia magna, Water flea 2 378 9479 

Daphnia magna, Water flea 2 630 9479 

Daphnia magna, Water flea 2 740 9479 

Daphnia magna, Water flea 2 831.6 87973 

Daphnia magna, Water flea 2 945.25 80944 

Daphnia magna, Water flea 2 1174.1 87973 

Daphnia magna, Water flea 2 1509.36 87973 

Daphnia magna, Water flea 2 2077.6 87973 

Diaptomus sp., Calanoid copepod 2 0.6 0.6 786 

Eretes sticticus, Beetle 2 10 10 5182 

Eucyclops sp., Cyclopoid copepod 2 0.1 0.1 786 

Gammarus faciatus, Scud 4 6 6 submitted 

Gammarus lacustris, Scud 4 5.8 5.8 submitted 

Hyalella azteca, Scud 4 2.6865 4.39 87973 

Hyalella azteca, Scud 4 5.643 87973 

Hyalella azteca, Scud 4 5.586 87973 

Hydra viridissima, Hydra 4 670 670 20380 

Hydra vulgaris, Hydra 4 810 810 20380 

Jappa kutera, Mayfly 4 2.3 1.3 62479 

Jappa kutera, Mayfly 4 0.7 62479 

H-5
Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * 

Jappa kutera, Mayfly 4 1.2 62479 

Jappa kutera, Mayfly 4 1.8 62479 

Lamellidens marginalis, Mussel 2 43410 43410 126 

Lanistes carinatus, Snail 1 3400 3400 12022 

Lymnaea luteola, Pond snail 4 60 60 103723 

Lymnaea natalensis, Pond snail 2 4370 4370 11147 

Macrobrachium rosenbergii, Giant river prawn 4 0.93 0.43 61993 

Macrobrachium rosenbergii, Giant river prawn 4 0.2 61993 

Moina micrura, Water flea 1 16.2 16.2 87649 

Oziotelphusa senex, Crab 2 6200 6200 18025 

Palaemonetes paludosus, Riverine grass shrimp 4 0.24 0.24 93458 

Paramelita nigroculus, Amphipod 1 19.2 19.2 87478 

Paratya australiensis, Shrimp 2 0.96 0.70 103213 

Paratya australiensis, Shrimp 2 0.51 103213 

Procambarus clarkii, Red swamp crayfish 4 24 24 12802 

Pseudagrion sp., Dragonfly 1 46 46 12022 

Pteronarcys californica, Stonefly 4 2.3 2.3 submitted 

Spicodiaptomus chilospinus, Calanoid copepod 2 40 40 5264 

Tigriopus japanicus, Harpacticoid copepod 4 69.93 69.9 104287 

* The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # 

labeled “submitted” refers to data submitted by the registrant, and can be found in Appendix E. 

Table H-3. The LC50 values used for the estuarine/marine fish species sensitivity distribution in 

Figure 5.5. 

Estuarine/Marine Fish Species Dur (d) LC50 (ug/L) SMAV Ref * 

Cymatogaster aggregata, Shiner perch 4 1.1 1.08 3752 

Cymatogaster aggregata, Shiner perch 4 1.06 2264 

Cyprinodon variegatus, Sheepshead minnow 4 0.34 1.38 3740 

Cyprinodon variegatus, Sheepshead minnow 4 0.6 3740 

Cyprinodon variegatus, Sheepshead minnow 4 0.71 3740 

Cyprinodon variegatus, Sheepshead minnow 4 0.83 3740 

Cyprinodon variegatus, Sheepshead minnow 4 0.88 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.1 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.15 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.19 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.2 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.4 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.4 3740 

Cyprinodon variegatus, Sheepshead minnow 4 1.61 3740 

Cyprinodon variegatus, Sheepshead minnow 4 2.5 3740 

Cyprinodon variegatus, Sheepshead minnow 4 2.7 3740 

Cyprinodon variegatus, Sheepshead minnow 4 2.74 3740 

H-6
Estuarine/Marine Fish Species Dur (d) LC50 (ug/L) SMAV Ref * 

Cyprinodon variegatus, Sheepshead minnow 4 2.81 3740 

Cyprinodon variegatus, Sheepshead minnow 4 2.87 3740 

Cyprinodon variegatus, Sheepshead minnow 4 3.45 3740 

Fundulus heteroclitus, Mummichog 4 1.45 0.93 95846 

Fundulus heteroclitus, Mummichog 4 1.82 3726 

Fundulus heteroclitus, Mummichog 4 0.31 3726 

Fundulus heteroclitus, Mummichog 4 0.91 73399 

Lagodon rhomboides, Pinfish 4 0.3 0.3 submitted 

Leiostomus xanthurus, Spot 4 0.09 0.09 submitted 

Mugil cephalus, Striped mullet 4 0.38 0.38 submitted 

Morone saxatilis, Striped bass 4 0.1 0.1 submitted 

* The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # 

labeled “submitted” refers to data submitted by the registrant, and can be found in Appendix E. 

Table H-4. The LC50 values used for the estuarine/marine invertebrate species sensitivity 

distribution in Figure 5.6.

Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * 

Americamysis bahia, Opossum shrimp 4 0.24 0.78 3740 

Americamysis bahia, Opossum shrimp 4 0.34 3740 

Americamysis bahia, Opossum shrimp 4 0.36 3740 

Americamysis bahia, Opossum shrimp 4 0.38 3740 

Americamysis bahia, Opossum shrimp 4 0.46 3740 

Americamysis bahia, Opossum shrimp 4 0.73 3740 

Americamysis bahia, Opossum shrimp 4 0.75 3740 

Americamysis bahia, Opossum shrimp 4 0.94 3740 

Americamysis bahia, Opossum shrimp 4 1.04 3740 

Americamysis bahia, Opossum shrimp 4 1.04 3740 

Americamysis bahia, Opossum shrimp 4 1.12 3740 

Americamysis bahia, Opossum shrimp 4 1.16 3740 

Americamysis bahia, Opossum shrimp 4 1.29 3740 

Americamysis bahia, Opossum shrimp 4 1.47 3740 

Americamysis bahia, Opossum shrimp 4 1.52 3740 

Americamysis bahia, Opossum shrimp 4 1.77 3740 

Artemia parthenogenetica, Brine shrimp 1 1890.5 4071 20398 

Artemia parthenogenetica, Brine shrimp 1 8768.5 20398 

Artemia salina, Brine shrimp 1 2688.5 2689 20398 

Brachionus plicatilis, Rotifer 1 5600 6432 12646 

Brachionus plicatilis, Rotifer 1 6600 12646 

Brachionus plicatilis, Rotifer 1 7200 12646 

Callinectes sapidus, Blue crab 5-6 125 237 67696 

Callinectes sapidus, Blue crab 6-8 450 18705 

Cancer magister, Dungeness or edible crab 4 14.8 14.8 3752 

H-7
Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * 

Crassostrea madrasensis, Oyster 4 14.13 14.13 14270 

Crassostrea virginica, Oyster 4 0.45 0.45 submitted 

Dinophilus gyrociliatus, Archiannelid 4 933.5 1029 11940 

Dinophilus gyrociliatus, Archiannelid 4 1135 11940 

Gammarus palustris, Gammarid amphipod 4 0.43 0.48 51439 

Gammarus palustris, Gammarid amphipod 4 0.54 51439 

Litopenaeus stylirostris, Blue Shrimp 2 235 235 73317 

Macrophthalmus erato, Mangrove crab 4 0.48 0.48 69820 

Nanosesarma sp., Crab 4 31 31 18580 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.12 0.77 73399 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.18 3726 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.206 61089 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.28 3726 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.303 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.31 3726 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.337 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.39 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.513 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.563 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.583 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.62 61089 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.62 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.63 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.641 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.645 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.73 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.894 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.911 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.92 61089 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.96 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.96 18932 

Palaemonetes pugio, Daggerblade grass shrimp 4 0.97 18932 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.01 95846 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.01 18932 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.31 submitted 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.342 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.397 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.4 8613 

Palaemonetes pugio, Daggerblade grass shrimp 4 1.99 61089 

Palaemonetes pugio, Daggerblade grass shrimp 4 2.56 71986 

Palaemonetes pugio, Daggerblade grass shrimp 4 117 71986 

H-8
Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * 

Penaeus duorarum, Northern pink shrimp 4 0.04 0.04 submitted 

Penaeus sp., Penaeidean shrimp 2 0.18 0.24 3726 

Penaeus sp., Penaeidean shrimp 2 0.31 3726 

Sagitta sp., Chaetognaths 1 424 424 2658 

Uca pugnax, Fiddler crab 4 790 790 submitted 

* The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # 

labeled “submitted” refers to data submitted by the registrant, and can be found in Appendix E. 

II. Species Sensitivity Distribution Equations 

Cumulative species sensitivity distribution (SSD) curves were created and evaluated in a manner 

similar to that of Carriger and Rand (2008) using a log-logistic distribution. Each data point 

reflects the geometric mean of the acute toxicity values within a species. The formula used for 

calculating the SSD within each taxonomic group is: 

X

X

e

e

p βα

βα

+

+

+ =1 (Equation 1) 

where: 

p = cumulative % of species affected; 

X = log10(species LC50); and 

α and β = scale parameters (Warren-Hicks et al. 2002) 

The linear form of the equation 1 is written as: 

X

p

p += βα ⎟

⎟

⎠

⎞

⎜

⎜

⎝

⎛

1− ln (Equation 2) 

Source: Carringer and Rand (2008)

Based on the above information, parameters log-logistic regression equations were determined 

maximizing the R2

 between the observed and calculated LC50 values using the Microsoft® Excel 

“Solver” application (Table H-5). 

Table H-6. Parameters for the log-logistic regression models for acute toxicity for various 

taxonomic groups. 

Taxa α β

Freshwater Fish -0.952 2.434 

Freshwater Invertebrates -1.313 0.937 

Saltwater Fish 0.969 2.506 

Saltwater Invertebrates -1.049 0.750 

Aquatic Amphibians—4 da -2.221 1.525 

Aquatic Amphibians—8 da -2.064 2.095 

H-9
III. Joint Probability Curves 

Joint probability curves (JPC) were created by using concentrations that encompassed the range 

of acute toxicity values for each SSD. For each concentration, the percentage of LC50 values that 

were exceeded was calculated based on the SSD regression for each taxonomic group. 

Simultaneously, the number of exposure concentrations for each application scenario that were 

equal to or greater than each of these concentrations was determined. This resulted in a series of 

data pairs for each exposure/taxa combination which were used to generate the JPC for each 

taxonomic group. Results for selected LC50 exceedence percentiles are shown in Tables H-6 

through H-9. 

Table H-6. Probabilities that estimated concentrations of endosulfans exceed a given 

percentage of species-mean LC50 values for freshwater fish.

Percentage of Species-Mean LC50s Exceeded Crop Use 

Category 

Aerial or 

Ground 

Application 

Crop Exposure 

Scenario 5% 10% 50% 90% 

A IL Beans 0.99 0.98 0.08 0 

G IL Beans 0.98 0.98 0.08 0 

A WA Beans 0.58 0.08 0 0 

Beans G WA Beans 0.08 0.03 0 0 

A FL Citrus 0.97 0.94 0.14 0 

Citrus* G FL Citrus 0.96 0.93 0.13 0 

G CA Cotton 0.98 0.39 0 0 

A S. TX Cotton 0.98 0.91 0.03 0 

Cotton G S. TX Cotton 0.98 0.93 0.05 0 

G CA Melon 0.31 0.08 0 0 

Cucurbits G S. TX Melon 0.97 0.89 0.03 0 

Eggplant* G CA Eggplant 0.01 0 0 0 

A MI Cherry 0.98 0.92 0.02 0 

Fruits A WA Orchards 0.11 0.05 0 0 

Lettuce G CA Lettuce 0.89 0.64 0.01 0 

A ME Potato 0.98 0.97 0.03 0 

G ME Potato 0.98 0.97 0.02 0 

A WA Potato 0.96 0.39 0 0 

Potato* G WA Potato 0.84 0.15 0 0 

Tobacco G NC Tobacco 0.97 0.71 0.01 0 

G CA Tomato 0.08 0.01 0 0 

A FL Tomato 0.98 0.95 0.03 0 

A PA Tomato 0.99 0.98 0.05 0 

Tomato* G PA Tomato 0.98 0.98 0.04 0 

* = exposure scenarios illustrated in Section 5.2.2.3 

Table H-7. Probabilities that estimated concentrations of endosulfans exceed a given 

percentage of species-mean LC50 values for freshwater invertebrates.

Crop Use Percentage of Species-Mean LC50s Exceeded 

Category 

Aerial or Ground 

Application 

Crop Exposure 

Scenario 5% 10% 50% 90% 

A IL Beans 0.99 0.99 0 0 

G IL Beans 0.99 0.98 0 0 

A WA Beans 0.98 0.84 0 0 

Beans G WA Beans 0.96 0.16 0 0 

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Crop Use Aerial or Ground Crop Exposure Percentage of Species-Mean LC50s Exceeded 

Category Application Scenario 5% 10% 50% 90% 

A FL Citrus 0.98 0.98 0 0 

Citrus* G FL Citrus 0.98 0.97 0 0 

G CA Cotton 0.98 0.98 0 0 

A S. TX Cotton 0.99 0.98 0 0 

Cotton G S. TX Cotton 0.98 0.98 0 0 

G CA Melon 0.97 0.51 0 0 

Cucurbits G S. TX Melon 0.99 0.98 0 0 

Eggplant* G CA Eggplant 0.77 0.02 0 0 

A MI Cherry 0.99 0.99 0 0 

Fruits A WA Orchards 0.99 0.31 0 0 

Lettuce G CA Lettuce 0.97 0.95 0 0 

A ME Potato 0.98 0.98 0 0 

G ME Potato 0.98 0.98 0 0 

A WA Potato 0.99 0.98 0 0 

Potato* G WA Potato 0.99 0.94 0 0 

Tobacco G NC Tobacco 0.99 0.98 0 0 

G CA Tomato 0.97 0.14 0 0 

A FL Tomato 0.99 0.99 0 0 

A PA Tomato 0.99 0.99 0 0 

Tomato* G PA Tomato 0.99 0.98 0 0 

* = exposure scenarios illustrated in Section 5.2.2.3 

Table H-8. Probabilities that estimated concentrations of endosulfans exceed a given 

percentage of species-mean LC50 values for estuarine/marine fish.

Percentage of Species-Mean LC50s Exceeded Crop Use 

Category 

Aerial or 

Ground 

Application 

Crop Exposure 

Scenario 5% 10% 50% 90% 

A IL Beans 0.99 0.99 0.98 0.04 

G IL Beans 0.99 0.98 0.98 0.04 

A WA Beans 0.98 0.98 0.06 0 

Beans G WA Beans 0.91 0.62 0.02 0 

A FL Citrus 0.98 0.98 0.92 0.07 

Citrus* G FL Citrus 0.98 0.97 0.92 0.06 

G CA Cotton 0.98 0.98 0.19 0 

A S. TX Cotton 0.99 0.99 0.8 0.02 

Cotton G S. TX Cotton 0.98 0.98 0.88 0.03 

G CA Melon 0.97 0.91 0.05 0 

Cucurbits G S. TX Melon 0.98 0.98 0.78 0.02 

Eggplant* G CA Eggplant 0.44 0.12 0 0 

A MI Cherry 0.99 0.99 0.8 0.01 

Fruits A WA Orchards 0.99 0.95 0.03 0 

Lettuce G CA Lettuce 0.97 0.97 0.51 0.01 

A ME Potato 0.98 0.98 0.96 0.01 

G ME Potato 0.98 0.98 0.93 0.01 

A WA Potato 0.99 0.99 0.19 0 

Potato* G WA Potato 0.99 0.98 0.03 0 

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Tobacco G NC Tobacco 0.98 0.98 0.38 0 

G CA Tomato 0.96 0.57 0.01 0 

A FL Tomato 0.99 0.99 0.92 0 

A PA Tomato 0.99 0.99 0.98 0.02 

Tomato* G PA Tomato 0.99 0.98 0.96 0.02 

* = exposure scenarios illustrated in Section 5.2.2.3 

Table H-9. Probabilities that estimated concentrations of endosulfans exceed a given 

percentage of species-mean LC50 values for estuarine/marine invertebrates.

Crop Use Percentage of Species-Mean LC50s Exceeded 

Category 

Aerial or Ground 

Application 

Crop Exposure 

Scenario 5% 10% 50% 90% 

A IL Beans 0.99 0.99 0 0 

G IL Beans 0.99 0.99 0 0 

A WA Beans 0.98 0.98 0 0 

Beans G WA Beans 0.98 0.88 0 0 

A FL Citrus 0.98 0.98 0 0 

Citrus* G FL Citrus 0.98 0.98 0 0 

G CA Cotton 0.98 0.98 0 0 

A S. TX Cotton 0.99 0.99 0 0 

Cotton G S. TX Cotton 0.99 0.98 0 0 

G CA Melon 1 0.97 0 0 

Cucurbits G S. TX Melon 0.99 0.98 0 0 

Eggplant* G CA Eggplant 0.97 0.38 0 0 

A MI Cherry 0.99 0.99 0 0 

Fruits A WA Orchards 0.99 0.99 0 0 

Lettuce G CA Lettuce 1 0.97 0 0 

A ME Potato 0.98 0.98 0 0 

G ME Potato 0.98 0.98 0 0 

A WA Potato 0.99 0.99 0 0 

Potato* G WA Potato 0.99 0.99 0 0 

Tobacco G NC Tobacco 0.99 0.98 0 0 

G CA Tomato 0.99 0.95 0 0 

A FL Tomato 0.99 0.99 0 0 

A PA Tomato 0.99 0.99 0 0 

Tomato* G PA Tomato 0.99 0.99 0 0 

* = exposure scenarios illustrated in Section 5.2.2.3 

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IV. References 

Carriger, JF, Rand, GM. 2008. Aquatic risk assessment of pesticides in surface waters in and 

adjacent to the Everglades and Biscayne National Parks: II Probabilistic analyses. 

Ecotoxicology. 17:680-96. 

Warren-Hicks, WJ, Parkhurst, BR and Butcher, JB. 2002. Methodology for Aquatic Ecological 

Risk Assessment. pp. 345-382 (in) L. Posthuma, GW Suter II and TP Traas. Species Sensitivity 

Distributions in Ecotoxicology. Lewis Publishers. 587 pp. 

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