### SAT Report PMN Number: P-10-0005 SAT Date: 12/8/2009 Print Date: 12/2/2013

Related cases :

Concern levels : Type of Concern: Health Eco Comments Level of Concern: 2 1

Persistence Bioaccum Toxicity Comments 3 31

Exposure Based Review : Health: N o Ecotox: N o
Routes of exposure : Health: Dermal Inhalation Ecotox: N o releases to water Fate: ; Keywords : Keywords: LUNG, ONCO, IMMUNO
Summary of Assessment :

Fate : Fate Summary: P-10-0005 FATE: Solid S < 0.001 mg/L at 25 EC (E) VP < 1.0E-6 torr at 25 EC (E) BP > 400 EC (E) H < 1.00E-8 (E) POTW removal (%) = 0-90 via sorption Time for complete ultimate aerobic biodeg > mo Sorption to soils/sediments = low - v.strongPBT Potential: P3B3 *CEB FATE: Migration to ground water = negl - rapid Direct photolysis will be negl - rapid Indirect photolysis will be negl - rapid

Health : Health Summary: Absorption is poor all routes based on analogs. There is concern for lung toxicity if inhaled based on lung overload for poorly soluble particulates and concern for oncogenicity and immunotoxicity based on carbon nanotubes. Moderate concern.

Ecotox :
Test Organism Test Type Test End Point Predicted Measured Comments

fish 96-h LC50 * * invalid effluent test daphnid 48-h LC50 * * invalid effluent test green algal 96-h EC50 * fish

_ chronic value * daphnid

_ chronic value *

algal

_ chronic value *
Sewage Sludge 3-h EC50

_ Sewage Sludge

_ Chronic Value _

Ecotox Values Comments: Predictions are based on SARs for polynonionic polymers; SAR chemical class = p olyme r -nonionic-C84 nanotube; MW>10 000; log Kow >> 8 (P); solid with mp unk but S << 0.001 mg/L at 25 C (P); p H7; effective concentrations based on 100% active ingredients and nominal concentrations; hardness <150.0 mg/L as CaCO3; and TOC <2.0 mg/L;
This PMN was submitted with two non-standard ecotoxicity tests with fish and daphnia. The tests are of plant effluents with only one concentration of effluent tested with the addition of a small amount of this single-walled carbon nanotube PMN. Conclusions are shown below. Details of the analysis are given in the attached spreadsheet.
These two assays were plant effluent tests rather than acute base-set tests and are inadequate to characterize the toxicity of this single-walled carbon nanotube (SWCNT) PMN. Specifically, they cannot be used to set surface water concern concentrations. Only one concentration of SWCNT was tested. Ceriodaphnia and fish (fathead minnows) were exposed separately for 7 days to plant effluent at different dilutions (0, 0.5, 1, 5, 32%). An additional group consisted of 5% effluent into which was mixed this SWCNT at a nominal loading concentration of 0.023%. The organisms showed no differences in effects (survival and growth for fish - survival and reproduction for daphnia) when exposed to 0.023% SWCNT in 5% effluent than when exposedto 5% effluent alone. N o explanation of how SWCNTs were introduced into the effluent was given (e.g., mixing, sonication, use of solvents, filtering, etc.). In these two assays, no effects could be attributed to CNT. This is in agreement with ECOSAR predictions for nonionic p olymers with physicochemical characteristics of this SWCNT. However, because these tests were so limited in their documentation and other major aspects, they are considered effectively invalid and ECOSAR predictions are relied upon to regulate this PMN.

Factors Values Comments Assessment Factor 10 Concentration of Concern ( p pb) *

SARs nonionic polymers SAR Class p olyme r -nonionic-carbon allotrope Ecotox Category

Ecotox Factors Comments:

SAT Chair: Becky Jones