# Site

- Specific Information in Support of Establishing
Numeric Nutrient Criteria in

the Tolomato

– Matanzas Estuary

Division of Environmental Assessment and Restoration
Standards and Assessment Section Florida Department of Environmental Protection Tallahassee, FL 32399

August 25, 2010Draft ii August 2010 

### Table of Contents

Executive Summary

............................................................................................................... 1

Sources of Nutrients

.............................................................................................................. 7
Biological Summary

............................................................................................................... 7

Salt Marsh

................................................................................................ .................................... 8
Mollusk Reefs

................................................................................................ ................................ 8

Plankton

................................................................................................ ....................................... 9


### Water Quality Data Sources and Studies

................................................................................ 9
GTMNERR Systemwide Monitoring Program (SWMP)

.................................................................... 9
St Johns River Water Management District (SJRWMD) Surface Water Quality Monitoring (SWQM)

................................................................................................ ...................................... 11 

### Waters on the 303(d) List

..................................................................................................... 12

Numeric Nutrient Criteria Recommendation

........................................................................ 17

Lines of Evidence

................................................................................................................. 18

Chla – [TN] or [TP] Regression Models

.......................................................................................... 18

General Models

........................................................................................................................... 18

Results and Discussion

................................................................................................................. 19


### Summary and Conclusions

................................................................................................... 40

References

.......................................................................................................................... 44Draft iii August 2010 

### List of Tables

Table 1a. Tolomato

- Matanzas Estuary, concentration limits: Annual median (mean), 2000

– 09.

............. 1

Table 1 b . Tolomato

- Matanzas Estuary, loading limits: Annual median (mean), 2000

– 09.

........................ 2
Table 1. Checklist of nutrient enrichment symptoms in the TME.

................................................................ 3
Table 3. Estuarywide annual averages based on average annual site geometric means.

.......................... 9
Table 5. Selected water quality parameters in the Upper East Coast Basin (1990

- 2008).

........................ 11
Table 6. Comparison of nutrient levels in the Tolomato

- Matanzas estuary and Pellicer Creek to NOAA (1996) and FDEP (1996) state standards.

......................................................................... 12

Table 7. 303(d) waterbodies status for the Northern Coastal Basin extracted from the FDEP revised draft Verified Lists of impaired waters for the Group 5 Basins current as of 9/21/2007*.

................................................................................................ ................................ . 12

Table 8a. Tolomato

- Matanzas Estuary, reference period results –1 st line of evidence: Annual median (mean), 2000

– 09.

........................................................................................................... 33

Table 8b. Tolomato

- Matanzas Estuary, reference period results

– 1st line of evidence: Maximum

wet

- season median (mean) (June

– September).

.......................................................................... 34

Table 9. Tolomato

- Matanzas Estuary, linear regression results –2 nd line of evidence: Maximum

wet

- season median (1986

– 2009; all months).

............................................................................ 35
Table 10. Estimates of current external loadings of TN and TP to Tolomato

- Matanzas Estuary (2004 land use, 2001

– 2008 avg. annual rainfall of 48”).

............................................................ 38
Table 12a. Summary of lines

- of

- evidence results: loading and concentration limits for the Tolomato segment.

..................................................................................................................... 41
Table 12b. Summary of lines

- of

- evidence results: loading and concentration limits for the North Matanzas segment.

..................................................................................................................... 41
Table 12c. Summary of lines

- of

- evidence results: loading and concentration limits for the South Matanzas segment.

..................................................................................................................... 41



### List of Figures

Figure 1. Guana

- Tolomato

- Matanzas Estuary and Planning Units.

............................................................ 6
Figure 2. Monthly TN values at the four GTMNERR monitoring stations for 2002

- 2006.

......................... 10
Figure 3. TP at GTMNERR monitoring stations for 2002

- 2008 from north to south (2007

- 2008 data provided directly from NERR – not verified by NOAA).

....................................................... 10
Figure 4. Monthly chlorophyll a values at the four GTMNERR monitoring stations for 2002

- 2006.

......... 10
Figure 5. Tolomato River Planning Unit, including 1998 303(d) list and Planning List waters (FDEP 2005).

................................................................................................ ................................ 14
Figure 6. Matanzas River Planning Unit, including 1998 303(d) list and Planning List waters.

................. 15
Figure 7. Pellicer Creek Planning Unit, including the 1998 303(d) list and Planning List waters.

.............. 16
Figure 8. Locations of water quality sampling sites monitored by the agencies that provided data used in this nutrient criteria analysis. The SJRWMD and GTMNERR provided the bulk of water quality data. Only salinity data were retrieved from the SEAS database for analysis.

................................................................................................ ....................................... 17Draft iv August 2010 Figure 9. Percent composition of major land uses in TME: Tolomato, N. Matanzas, and S.

Matanzas (based on 2004 aerial imagery).

................................................................................. 20
Figure 10a. Location of tidal node at boundary between the TME and Lower St. Johns River

Estuar y.

................................................................................................ ........................................ 21
Figure 10b. Location of tidal node at near Crescent Beach Bridge, midway between St. Augustine
Inlet to the north and Matanza s Inlet to the south.

.................................................................... 21
Figure 10c. Location of tidal node at boundary between the Matanzas and Halifax River

Estuaries.

................................................................................................ ..................................... 22
Figure 11. Spatial distribution of salinity and turbidity (mean and std. deviation) by sampling site with median levels by segment included (1986

– 2009 period of record). The SEAS/FDACS sites are included only in the salinity graph; both graphs include the

SJRWMD and GTMNERR sites.

.................................................................................................... 23
Figure 12. Spatial distribution of TN, TP, and Chla (mean ± std deviation) by SJRWMD or GTMNERR sampling site with segment mean and median included (1986

– 2009 period of record).

................................................................................................ .................................... 24
Figure 13. Spatial distribution of TSI values by SJRWMD or GTMNERR sampling site with segment mean and median included (1986

– 2009 period of record).

......................................... 25
Figure 14. Time series graphs of annual mean [TN] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

.................................................................... 29
Figure 15. Time series graphs of annual mean [TP] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

.................................................................... 30
Figure 16. Time series graphs of annual mean [TP] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

.................................................................... 31
Figure 17. Time series graphs of annual mean [Chla] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomat o segments.

.................................................................... 32
Figure 18. Time series graphs of annual mean Trophic State Index (TSI) values ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

................................... 33
Figure 19. Average monthly [TN], [Chl a], and [TP] in the TME overall. Monthly rainfall data are shown for comparison. The 1986 – 2009 period was used for the average monthly water quality data; and the 1986 – 2008 period was used for average monthly rainfall.

.......... 36
Figure 20. Mean turbidity by year (1992

– 2008) for TME in comparison to the North and South Halifax River Estuary (N. HRE and S. HRE) and the Indian River Lagoon (IRL).

........................... 37
Figure 21. The log

- linear plot of TN and TP load limits (g/m 2 /yr) vs. hydraulic loading (q

s ) adapted and improved from Steward and Lowe (2010) by the incorporation of additional data points from mesotrophic estuaries initially used to validate the models. A point lying above the 95% confidence interval for the line

- of

- best

- fit (=upper mestrophic line or UML) is considered eutrophic; below the lower mesotrophic line (LML) is considered oligotrophic.

................................................................................................ . 39Draft 1 June 2010 

### Executive Summary

This report was prepared by the Florida Department of Environmental Protection (FDEP), in cooperation with local scientists, to support the development of num eric nutrient criteria for the Tolomato Matanzas Estuary (TME)

. The primary purpose of the proposed numeric nutrient criteria is to protect healthy well

- balanced natural populations of flora and fauna from the effects of excess nutrient
enrichment. Available information demonstrates that biological communities in this coastal area are healthy and wel l balanced. There are extensive salt marshes and mollusk reefs and a high diversity of plants and animals, including many protected species. The evidence presented in this document shows that aquatic life designated use in the TME is fully supported. FDEP therefore proposes that the n umeric nutrient criteria be crafted to maintain the existing nutrient regime.

The TME is a n 80

- kilometer

- long tidal estuary located on the Atlantic coast near the city of St Augustine

, its major ocean connection s are the St . Augustine and Matanzas Inlets ( Figure 1). The basin morphology is typical of the Florida east coast, with a series of flat, low

- gradient terraces separated by ancient barrier beaches (Scholl et al. 1980).

Nutrients to the TME originate from natural sources (leaf litter inputs from th e extensive forests and marshes),as well as from stormwater runoff, agriculture, silviculture, urban/suburban land uses, atmospheric deposition, sediment loading and turbidity from shoreline erosion, septic tanks, and wastewater treatment facilities (WWTFs ).

Oyster beds are the primary sensitive aquatic resource in the TME , roughly analogous in form and function to seagrasses in other estuaries throughout Florida. Unfortunately, there is no functional relationship between nutrient levels and oysters (as there is with seagrasses) that would be useful for setting nutrient limits in the estuary. In fact, additional nutrient loads may actually increase oyster production. Therefore, FDEP has evaluated multiple lines of evidence associated with the trophic

stat e/chlorophyll a /nutrient relationship in the TME to develop nutrient criteria that would maintain designated uses. The three lines of evidence that were used in developing numeric nutrient criteria for
the TME include:

**•** a r eference period method;

**•** results from c hlorophyll a – nutrient regression analyses ; and

**•** t wo estuary empirical models (Steward et al. 2010a).

For the purposes of developing numeric nutrient criteria, the TME was divided into three segments: the Tolomato River (including the Guana River system) segment, and a north and south Matanzas River segment (Steward et al. 2010a). Segmentation was based on local land uses and hydrodynamic properties, particular ly water residence times

, which mediate water quality and trophic response.
Tables 1a an d 1b summarize the proposed concentration and loading limits for the Tolomato, N. Matanzas, and S. Matanzas segments, respectively.

Table 1a

. Tolomato

- Matanzas Estuary, concentration limits: Annual median (mean), 2000

– 09

. **Segment Chla (μg/L)**  **TN (mg/L) TP (mg/L)** 

Tolomato 4.5 (5.3) 0.52 (0.56) 0.085 (0.096)

N. Matanzas 3.1 (3.5) 0.37 (0.41) 0.073 (0.083)

S. Matanzas 4.3 (5.0) 0.45 (0.49) 0.089 (0.103)Draft 2 June 2010 Table 1b. Tolomato

- Matanzas Estuary loading limits (ca. 2004 loadings) for TN and T P.

- = Empty cell/no data **Segment** TN: kg/yr TP: kg/yr

Tolomato segment TN: kg/yr TP: kg/yr
- Loading Limit 76,727 11,264

N. Matanzas segment - - Loading Limit 237,074 43,792 S. Matanzas segment - - Loading Limit 208,025 37,142Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 3 August 2010 Table 2

. Checklist of nutrient enrichment symptoms in the TME.

- = Empty cell/no data Response variable Observed historically? Observed currently? Explanation Source

Low DO (hypoxia/anoxia) Yes Yes Caused by natural factors, especially the h igh oxygen demand by decomposing bacteria in the highly organic saltmarsh systems. Steward and Miller, pers.

comm. 2010

Reduced clarity Yes Yes Naturally high turbidities and high tidal scouring contribute to sporadic

reductions in clarity. The naturally low clarity (from tripton) contributes to the lack of seagrass in this region of Florida. Steward et al . 2010a

Increased chlorophyll a

concentrations Yes No Slightly increasing temporal trend from 1986

- 2009 but annual averages well below the threshold for impairment in the IWR of 11 μg/l. Steward et al 2010 a

Phytoplankton bloom No No Short residence times, High tripton levels may help control phytoplankton
densities. Vertical mixing by tidal stirring may control phytoplankton blooms in some

estuaries. Steward et al 2010 a

Cloern 1991

Problematic epiphyte growth N/A N/A - -

Problematic macroalgal growth No No - -

SAV community changes or

loss N/A N/A Large tidal amplitude and naturally high turbidity have prevented SAV

establishment. Steward et al 2010 a

Emergent or shoreline vegetation community changes or loss Yes Yes I mpacts to shoreline habitats, associated with maintenance of inlets and navigation channels, mosquito control, and recreational boating activities

, have historically occurred, but are spatially limited. Haydt and Frazel 2003

Oyster reef community changes or loss Yes Yes Oyster reef communities have been negatively impacted by erosion, primarily due to wind and boat wakes and sediment loads due to runoff and tidal scouring.
It should be noted that d enser oyster populations

, with larger, meatier

individuals

, were found in areas with higher nutrient concentrations. Haydt and Frazel 2003

Dix and Ph lips 2009

Impacts to benthic community Unknown Unknown - -

Fish kills Yes Yes Fish kills reported January 2010 due to cold stress

Fish kill (red and black drum) reported August 2010 in San Sebastian Creek
(St. Augustine, Matanzas basin); cause unknown FFWCC 2010Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 4 August 2010 

### Executive Summary

................................................................................................................ 1
Geographical and Physical Description

................................................................................... 4

Sources and Fates of Nutrients

............................................................................................... 7

Po int Sources

................................................................................................ ................................ 7
Nonpoint Sources

.......................................................................................................................... 7


### Biological Summary

............................................................................................................... 7

Salt Marsh

................................................................................................ .................................... 8
Mollusk Reefs

............................................................................................................................... 8

Plankton

................................................................................................ ....................................... 9


### Water Quality Data Sources and Studies

................................................................................ 9
Waters on the 303(d) List

..................................................................................................... 12

Numeri c Nutrient Criteria Recommendation

........................................................................ 17
Reference period.

........................................................................................................................ 18

Chla – [TN] or [TP] regression models.

......................................................................................... 18

General models.

.......................................................................................................................... 18



### Results and Disc ussion

......................................................................................................... 19
Summary and Conclusions

................................................................................................... 40

References

........................................................................................................................... 42

Appendix A . List of Species 47Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 5 August 2010 

### Geographic al and Physical Description

The Tolomato

- Matanzas Estuary is located south of the City of Jacksonville, in St. Johns and Flagler Counties,
on the northeast coast of Florida. The estuary is characterized by extensive tidal marshes, unconsolidated substrates, and mollusk reefs (Frazel 2009). It is located in the Upper East Coast Drainage Basin and ha s been divided into four hydrologic planning units, three of which, the Tolomato River, Matanzas River and Pellicer
Creek (corresponding to the S. Matanzas river segment ) , are the subject of this report (Figure 1). The fourth unit, the Halifax River, is south of the TME boundary and is discussed in a separate report (Steward et al.

2 010b).

Much of the TME is located within the boundaries of the Guana

- Tolomato Estuarine Research Reserve (GTMNERR). The GTM NERR is geographically divided into a northern and southern component separated by the area around the City of St. Augustine and connected to the Atlantic Ocean via the St. Augustine and Matanzas Inlets. The natural hydrology of the basin has been significantly altered by a combination of water control structures, dikes, drainage ditches, and canals. In addition, the Intracoastal Wa terway traverses both
the Tolomato and Matanzas estuaries

The northern component of the GTM NERR is associated with the Tolomato and Guana River estuaries and has numerous associated managed areas including the Guana River Marsh Aquatic Preserve (GRMAP), Guana River Wildlife Management Area, Stokes Landing Conservation Area

, and Deep Creek State Forest. The GRMAP extends three miles into the Atlantic Ocean, and encompasses the estuarine waters of the Tolomato and Guana Rivers, interior impoundments, marshes, swamps and five artesian wells. The northern component also includes the upland areas of the former Guana River State Park.

The southern component of the GTMNERR is associated with the North Matanzas River estuary segment

, extending from Moultrie Creek s outh to Pellicer Creek. The southern component consists of Pellicer Creek Aquatic Preserve, Faver

- Dykes State Park, Washington Oaks Gardens State Park, Moses Creek Conservation Area, Pellicer Creek Conservation Area, Fort Matanzas National Monument, Prince ss Place Preserve, The River to Sea Preserve at Marineland, and other State sovereign submerged lands adjacent to the Matanzas River
within the GTM NERR boundary.

The Pellicer Creek watershed is associated with the South Matanzas River segment and consist s of vast wetlands, many miles of which were drained for pine plantations or real estate development, and to reduce mosquito breeding sites. Commercial shellfishing for oysters and clams has historically occurred in the southern portion of this planning unit.

The TME is roughly 80 kilometers long, and ranges in width from 0.32 km in Flagler County to 0.8 km in St. Johns County (Steward et al. 2010 a ). The total estuarine area is 44 km 2 and the watershed catchment area is
1, 024 km 2

. The catchment area to estuary ratio is 23:1, which is slightly less than that of the Halifax River Estuary (24:1; Steward et al. 2010 b ). The 30

- year a verage rainfall in the TME is 51 inches, although the average from 2001

- 2008

, which included a period of drought

, was 46 inches (S t eward et al. 2010 a ). The tidal amplitude (Scholl et al. 1980) is 1.25 meters

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 6 August 2010 Figure 1. Guana

- Tolomato

- Matanzas Estuary and Planning Units

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 7 August 2010 While a pproximately 73% of the basin currently consists of forest s and wetland s (Table 2), t he northeast
Florida region (Baker, Clay, Duval, Flagler, Nassau, Putnam and St. Johns counties) is one of the fastest growing
regions in the state. It has experienced a 22 percent increase in population between 1990 and 2000 (BERR,
University of Florida 2002) . By 2015, the region is expected to grow by an additional 20 percent.

Table 3. L and use in the Upper East Coast Basin (SJRWMD 2006).

Land Use Percent Coverage km 2

Forest 41 % 446.79

Wetland 32 % 356.65

Urban 15 % 192.14

Water 6% 66.94
Upland n on

- forested 4% 50.82

Transportation , Utilities, etc 1% 18.52
Agriculture 1% 6.82

Barren Land <1 % 1.14



### S ources of Nutrients

Potential sources of nutrients in the watershed include stormwater runoff, agriculture, silviculture, urban/suburban land uses, atmospheric deposition, sediment loading and turbidity from shoreline erosion, and septic tanks.

Septic tanks have been identified as a potential source of nutrients (N and P), pathogens, and other pollutants that can pose a threat to public health (SJRWMD 2003). Surface waters can be adversely affected by the direct or indirect runoff of septic system leachate. Septic tank failure rates in Flagler, St. Johns, and Volusia Counties exceed state failure rates (SJRWMD 2000).

Point Sources

There are 7 permitted domestic wastewater facilities th at discharge into the TME (FDEP 2006)

. Three facilities
are located in the N. Matanzas

, 3 in the S. Matanzas, and 1 in the Tolomato segment.

Six facilities in the Matanzas River Planning Unit have active NPDES permits to discharge to surface waters : four domestic waste treatment plants and two concrete batch plants. The most significant of the active facilities are the St. Augustine Wastewater Treatment Facility (WWTF) No. 1, with a design capacity of 5 mgd

, the Anastasia Island WWTF, with a design capacity of 4.0 mgd and the State Road 16 WWTF in St. Augustine, at 1.5 mgd

.

Nonpoint Source s

In the Tolomato River Planning Unit, upland forests, wetlands, and surface waters comprise about 81 percent
of the planning unit’s area. Approximately 13 percent of the area is urbanized, and about 5 percent is agricultural land or rangeland. According to the 2000 U.S. Census, St. Johns County is the second fastest

growing county in the state, and much of the new development will be near the coast, which will increase stormwater discharges to the I ntra -c oastal W ater way (SJRWMD 2003).



### Biological Summary

The TME contains a broad range of habitat types. Salt m arsh is the major tidal habitat, occupying approximately 25 percent of the total land cover of the GTM NERR. Patchy distributions of black mangrove,Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 8 August 2010 representing the northern extent of this species, a re also a significant feature of the marsh landscape in the
southern section of the GTM NERR. Other tidal communities include oyster bars, tidal flats and open water.

The diversity of communities present in the TME and surrounding lands provide habitat f or at least 44 mammal, 358 bird, 41 reptile, 21 amphibian, 303 fish, and 580 plant species (Apen dix A ). Many plants and animals receive special protection status , including the Anastasia Island beach mouse ( Peromyscus polionotus phasma ), gopher tortoise ( Gopherus polyphemus ), least tern ( Sterna antillarum ), marine turtles (loggerhead ( Caretta caretta ), leatherback ( Dermochelys coriacia ) and green turtle ( Chelonia mydas ), North Atlantic right whale ( Eubalaena glacialis ), the striped newt ( Notopthalmus perst riatus ), and the Atlantic geoduck ( Panopea
bitruncata ) among others

.

The spatial distributions of major aquatic habitat communities, oyster reef and salt marsh ( Spartina alterniflora

, Spartina patens , and Juncus roemerianus are the dominant marsh plant species) is fairly uniform throughout the TME (Johnson et al. 1998). Fortunately, the re has been very little loss of oyster reef or marsh area throughout the TME system (unlike the N. Halifax R. Estuary; see Steward 2010 b ). Most of the loss was due to dredge and fill development in the St. Augustine area up to the 1960’s (prime examples are downtown St. Augustine and the Davis Shores residential development on the opposite shore from downtown). The vast stretches of nearly contiguo us marsh, oyster habitat, and minimally developed upland strand in the TME, much of it publicly owned and under conservation management, is one reason why NOAA and the State of Florida established the GTMNERR. Another reason for establishing a NERR in thi s region is the exceptionally good water quality. The SJRWMD has petitioned FDEP to designate portions of the system as an Outstanding
Florida Water.

S alt Marsh

Salt marsh occup ies more than 20% of the total land cover of the TME (Frazel 2009) . Dominant plants in TME
salt marshes include saltmarsh cordgrass ( Spartina alterniflora ), black needlerush ( Juncus roemerianus ),

g lasswort ( Salicornia spp.), and saltwort ( Batis maritima ). This habitat supports a large population of infaunal organisms as well as a variety of transient fish and wildlife.

McFarlin (2008) assessed the impact of fertilization on food webs in 19 coastal estuaries in Georgia and found that fertilization increased cover, height, and biomass of Spartina and the height of Juncus

, but decreased

Juncus cover and biomass. The study also showed increases in herbivores (grasshoppers) with fertilization, but little to no effect on decomposers (fungi) or detritivores (snails). In comparisons between fertilized plots and control plots, variation among sites was typically greater than the effects of fertilization. These results suggest that increases in nutrients at any particular site could not readily be distinguish ed from the natural variation among sites

, and that increased nutrients are likely to have a stimulatory effect on desireable salt marsh plants ( Spartina ) and the herbivores that provide food for other predators

.

Mollusk Reefs

Oyster reefs are another major aquatic community in the TME

. They present a dynamic community and provide many benefits to the aquatic community

, including decreas ing turbidity by trapping sediment and forming refugia, nursery grounds, and feeding areas for a variety of other estuarine organisms. Oysters provide hard substrate and habitat for many other species including, mud crabs ( Rhithropanopeus harrisii ), blue crabs ( Callinectes sapidus ), and amphipods.

Dix (unpublished presentation 2010) conducted a three

- year study in the G T MNERR

, investigating oyster growth rates at two sites with different nutrient loading scenarios. Density, length, and biomass were compared between oyster colonies at a site near the San Sebastian River where the TN and TP loads were an order of magnitude or greater than a site near the Ft

. Matanzas Inlet. Although the lo ads were significantly different between the two sites, the mean total nitrogen concentrations (San Sebastian = 0.375 mg/L, Ft

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 9 August 2010 Matanzas = 0.365 mg/L) and total phosphorus (San Sebastian = 0.056 mg/L, Ft Matanzas = 0. 0 52 mg/L) were similar. Oyster biomass and abundance was greater at the San Sebastian site

, suggesting that the oyster communities are capable of assimilating current nutrient loads and appear to respond positively to increases in
nutrient loads

, at least at the current, benign levels.

Plankt on

Little research has been done on plankton communities within the TME

. However, Dr. Edward Phlips of the University of Florida is currently investigat ing phytoplankton structure and phytoplankton bloom density in a number of systems throughout Florida ( Frazel 2009)

.

Frazel noted a Karenia brevis in October 2007 within the waters of the TME

. However, t he bloom began in the
coastal waters between the mouths of the St. Johns and Nassau Rivers and mov ed south along the coast and

entered both the St. Augustine and Matanzas Inlets. Cell counts as high as 5,000,000 cells/L were recorded in the Matanzas River south of the 206 bridge (Hitchcock and Frazel, unpublished raw data).



### Water Quality Data Sources and Studies

The following organiza tions collect nutrient, bacteriologica l, and physical data in the TME :

**•** FL Dep t. of Env. Protection - Northeast District & Watershed Assessment ( 1 998 - 2010 )

**•** Guana

- Tolomato

- Matanzas N ational E stuarine R esearch R eserve ( 2002 - 2010 )

**•** FL Fish & Wildlife C C - Marine Research Institute ( 2001 - 2005 )

**•** St. Johns River Water Management District ( 1997 - 2010 )

**•** FL Dept. of Health - Bureau of Water ( 2000 - 2010 )

**•** FL Dept. of Agriculture and Consumer Services ( 1979 - 2007 )

GTM NERR System w ide Monitoring Program (SWMP)

Data from t he SWMP Program , which is conducted in conjunction with the National Oceanic and Atmospheric
Administration

, can be accessed via: http://cdmo.baruch.sc.edu/

. There are four monitoring stations in the

GTMNERR

, with two in the northern component and two in the southern component . At e ach station, physical , weather and nutrient/chlorophyll parameters are monitored

. Figure s 2-4 show the annual geometric means for total phosphorus, total nitrogen, and chlorophyll a , respect i vely at the four monitoring stations for May 2002 through November 2006. Table 3 shows the a nnual geometric means, standard deviations, and 75 th
/ 90 th percentiles for the four GTM NERR SWMP stations for 2002 through 2006.

Table 4. Estuary wide annual averages based on average annual site geometric means

.

Statistics Total Phosphorus (μg/L) Total Nitrogen (μg/L) Chlorophyll

- a (μg/L)

Mean 63.58 471.57 3.18

Std. Dev. 6.22 36.84 0.79

75 th percentile 67.8 496.4 3.7

90 th percentile 71.6 518.8 4.2Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 10 August 2010 Figure 2. Monthly TN values at the four GTM NERR monitoring stations for 2002

- 2006.

Figure 3. Monthly TP values at the four GTM NERR monitoring stations for 2002

- 200 8 (2007

- 2008 data not verified by NOAA).

Figure 4. Monthly chlorophyll a values at the four GTM NERR monitoring stations for 2002

- 2006. 0 0.5

1 1.5May-02Aug-02Nov-02Feb-03May-03Aug-03Nov-03Feb-04May-04Aug-04Nov-04Feb-05May-05Aug-05Nov-05Feb-06May-06Aug-06Nov-06mg/L Total Nitrogen Pine Island

San Sebastian

Fort Matanzas

Pellicer Creek

0 50 100 150 200 250May-02Oct-02Mar-03Aug-03Jan-04Jun-04Nov-04Apr-05Sep-05Feb-06Jul-06Dec-06May-07Oct-07Mar-08Aug-08μg/L Total Phosphorus

Pine Island

San Sebastian

Fort Matanzas

Pellicer Creek

0 5 10 15 20 25May-02Aug-02Nov-02Feb-03May-03Aug-03Nov-03Feb-04May-04Aug-04Nov-04Feb-05May-05Aug-05Nov-05Feb-06May-06Aug-06Nov-06 Chlorophyll a Pine Island

San Sebastian

Fort Matanzas

Pellicer CreekNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 11 August 2010 S t J ohns R iver Water M anagement D istrict (SJRWMD) Surface Water Quality Monitoring (SWQM)

The SJRWMD monitors water quality at 5 primary stations located either within, or directly adjacent to the

TME

. Data can be accessed at : http://sjrwmd.com/watershedfacts/factsindex.html

. Using GIS mapping technology, these measurements are evaluated in the context of po pulation density, geology, rainfall, and aquifer recharge. Table 5 summarizes nutrient data from the SJWMD Watershed Fact pages

.

Table 5

. Selected water quality parameters in the Upper East Coast B asin (1990

- 2008)

.

- = Empty cell/no data

Analytes Data Yrs n Data Min Median Max Range

TOLOMATO RIVER - - - - - - total nitrogen (mg/L as N) 16 140 0.13 0.59 1.82 mid

- lo
total phosphorus (mg/L as P) 16 139 0.02 0.09 1.24 mid
trichromatic uncorrected chlorophyll

- a (ug/L) 16 135 0.01 5.12 27.32 mid

- lo
trophic state index 16 138 -2.39 44.74 60.76 mid

- lo

UPPER MATANZAS RIVER - - - - - - total nitrogen (mg/L as N) 15 126 0.01 0.47 1.46 low
total phosphorus (mg/L as P) 15 124 0.02 0.07 0.61 mid
trichromatic uncorrected chlorophyll

- a (ug/L) 15 119 0.01 4.67 23.79 mid

- lo
trophic state index 15 124 -2.96 40.84 57.14 low

LOWER MATANZAS RIVER - - - - - - total nitrogen (mg/L as N) 16 134 -0.02 0.5 1.16 low
total phosphorus (mg/L as P) 16 134 0.02 0.07 0.27 mid
trichromatic uncorrected chlorophyll

- a (ug/L) 16 127 0.01 5.06 22.01 mid

- lo
trophic state index 16 133 -0.85 41.8 61.71 mid

- lo

PELLICER CREEK - - - - - - total nitrogen (mg/L as N) 16 83 0.1 0.83 2.11 low
total phosphorus (mg/L as P) 16 83 0.04 0.09 0.27 mid

trichromatic uncorrected chlorophyll

- a (ug/L) 16 86 -0.8 1.14 19.07 mid

- lo
water quality index 16 86 19.76 47.92 74.2 mid

- lo

MOULTRIE CREEK - - - - - - total nitrogen (mg/L as N) 15 85 0.43 1.28 4.04 mid

total phosphorus (mg/L as P) 15 85 0.02 0.07 0.3 mid

- lo

trichromatic uncorrected chlorophyll

- a (ug/L) 15 79 -0.19 0.58 26.4 low

water quality index 15 86 15.67 53.12 77.87 mid

In 2004, Miller analyzed the SJRWMD SWQM data from 1997 to 2000 for the Upper East Coast (SJRWMD refers to this region as the “Northern Coastal Basin”) and compared the results to National Oceanic and Atmospheric

Administration (NOAA) Estuarine Eutrophication Study (1996) and to the Florida water quality standard s or t he Typical Water Quality Values for Lakes, Streams, and Estuaries (FDER 1989 , Table 6)

. Miller found that t he
Tolomato

- Matanzas e stuary had good average dissolved oxygen (6.13 mg/L), relatively high salinity (30.42 ppt), and low average chlorophyll a (4.4 0 μg/L) (Miller 2004) . Mean total phosphorus concentration for this period (0.12 mg/L) was considered “High” compared to the 21 other estuarine systems on the southeast
Atlantic coast in the NOAA study and higher than the state median value of 0.07 mg/L (F DEP 1996)

. Mean total nitrogen concentration (0.57 mg/L) was classified as “Medium” and was lower than the state median concentration of 0.8 mg/L (FDEP 1996). The Trophic State Index (TSI) for the Tolomato

- Matanzas Estuary was 43.02, which is classified as “G ood”

. In addition, a ll individual sites within the estuary were classified as
“Good”.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 12 August 2010 Pellicer Creek, i n its middle reach at the U.S. 1 Bridge, had good average dissolved oxygen (5.06 mg/L), low average salinity (1.70 ppt), and average chlorophyll a concentration (6.27μg/L) (Miller 2004) . The a verage total phosphorus concentration (0.12 mg/L) was generally the same as found throughout the Tolomato

- Mat anzas estuary, and considered “H igh” when compared to other southeast Atlantic estuaries (NOAA 1996 ). Average total nitrogen concentration (0.94 mg/L) was considerably lower than the state median for fresh water (1.2 mg/L) but higher than in the Tolomato

- Matanzas estuary. The minimally disturbed Pellicer Creek is likely a major source of total nitroge n flowing into the Matanzas River. Pellicer Creek (at a depth of collection of 0.5 meters) is considered to be fresh water (specific conductivity < 5,000 μmhos), therefore, the Water Quality Index (WQI) was calculated to be 53.2, which is classified as “F air”

. The levels of fecal coliform (481.1 MPN/100 ml) and total coliform (1468.7 MPN/100 ml) calculated as geometric means, were above the Class II ( shellfish harvesting ) criteria and the Class III freshwater/marine recreational criteria, but the source of the bacteria
were not known , or even if they were of human origin (Miller 2004)

. Miller noted that the N:P ratio in the
Tolomato

- Matanzas system is 4.8, and is therefore nitrogen limited.

Table 6. Comparison of nutrient levels in the Tolomato

- Matanzas estuary and Pellicer Creek to NOAA (1996) and FDEP (1996) state standards

.

NOAA/FDEP Categories Total Nitrogen (mg/L) Total Phosphorus (mg/L) Chlorophyll a (μg/L) Trophic State Index (TSI) Water Quality Index (WQI)

Low / Good 0 to <0.1 0 to <0.01 >0 to 5 0 to <50 0 to <45

Medium / Fair 0.1 to <1 0.01 to <0.1 >5 to 20 50 to <60 45 to <60 High /Poor 1 0.1 >20 to 60 60 to 100 60 to 100 Hypereutrophic N ot applicable Not applicable >60 Not applicable Not applicable

Ceric ( 2007) analyzed the status and trends of water quality from 1990 to 2004 for all surface water basins in the SJRWMD, including the Upper East Coast

. The status was based on the median of annual medians using the most recent five years of available data (1999 to 2004). For the entire Upper East Coast , total nitrogen was 0.716 mg/L, total phosphorus was 0.0 97 mg/L, and chlorophyll a was 5.49 μ g/L. Compared to the NOAA
thresholds , this would suggest “Medi um” levels of TN

, TP

, and chlorophyll a in the basin.

Multiple modeling efforts have been undertaken or are ongoing within the TME to evaluate circulation, flushing, water quality conditions, and ecosystem health to meet water quality and habitat protection goals ( CDM/DHI 2002; Phlips 2004; Sheng 2008 ; Mao 2007 and 2009 )

.



### Waters on the 303(d) List

Table 7. 303(d) w aterbodies s tatus for the Northern Coastal Basin extracted from the FDEP r evised d raft Verified Lists of i mpaired w aters for the Group 5 Basins current as of 9/21/2007 *.

[there should be final lists available. Add text to describe listings, noting which ones are relevant to nutrients. Check into the listing for Lake Vedra. ]

- = Empty cell/no data *Complete dataset can be viewed at http://www.dep.state.fl.us/water/tmdl/verified_gp5R.htm

. Planning Unit/

Waterb ody Segment Planning Unit Waterb ody
Identification (WBID) Parameters Assessed Projected

Year of TMDL Development County

Tolomato/

Matanzas - - - - -

Guana River Tolomato River 2320 Coliform (Shellfish harvesting), Dissolved Oxygen, Fecal Coliform, Nutrients (Chla) 2012 St. Johns

Sombrero Creek Tolomato River 2470 Fecal Coliform 2017 St. Johns

Ximanies Creek Tolomato River 2477 Coliform (Shellfish harvesting classification) 2017 St. JohnsNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 13 August 2010 Lake Vedra Tolomato River 2320A Dissolved Oxygen 2012 St. Johns

Lake Vedra Tolomato River 2320A Nutrients (Chla) 2012 St. Johns

Tolomato River Tolomato River 2363I Arsenic, Coliform (Shellfish harvesting), Copper, Iron, Nickel 2017 St. Johns

Red House Br. Matanzas River 2472 Fecal Coliform 2017 St. Johns

Moultrie Creek Matanzas River 2493 Fecal Coliform 2017 St. Johns

Matanzas River Matanzas River 2363G Iron, Lead 2012 Flagler

Pellicer Creek

- - - - -

IWW Pellicer Creek 2363E Arsenic, Iron 2012 Flagler

IWW Pellicer Creek 2363F Coliform (Shellfish harvesting) 2017 Flagler

Pellicer Creek Pellicer Creek 2580B Fecal Coliform 2011 Flagler

Figures 5 through 7 show the individual p lanning u nits

, i ncluding the 1998 303(d) L l st, Planning List and
Verified List w aters

, point sources and additional features of interest (FDEP 2005).Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 14 August 2010 Figure 5. Tolomato River Planning Unit, i ncluding 1998 303(d) l ist and Planning List w aters (FDEP 2005)

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 15 August 2010 Figure 6. Matanzas River Planning Unit, i ncluding 1998 303(d) l ist and Planning List w aters

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 16 August 2010 Figure 7. Pellicer Creek Planning Unit, i ncluding the 1998 303(d) l ist and Planning List w aters

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 17 August 2010 

### Numeric Nutrient Criteria Recommendation

During the process of developing numeric nutrient criteria in the TME , both the spatial heterogeneity of the
system and temporal shifts in key water quality parameters were considered

. Based on a spatial analysis of TN, TP, chlorophyll a , salinity, turbidity, and Trophic State Index, Steward et al. (2010 a ) proposed dividing the

TME into three segments ( Figure 8). I nformation regarding the spatial distribution of physical properties and trophic water quality support a recommendation for dividing the TME into 3 segments – Tolomato , N. Matanzas, and S. Matanzas for the development of segment

- specific nutrient criteria.

Figure 8. Locations of wate r quality sampling sites monitored by the agencies that provided data used in this nutrient criteria analysis. The SJRWMD and GTMNERR provided the bulk of water quality data. Only salinity data were retrieved from the SEAS database for analysis

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 18 August 2010 

### Lines of Evidence for Criteria Development

Three lines of evidence were explored for the development of numeric nutrient criteria for the TME, including a reference period method, chlorophyll a

– nutrient regression analyses, and two estuary empirical models (Ste ward and Lowe 2010).

Reference P eriod

A reference period was selected to calculate annual and wet

- season medians of Chl a , TN, and TP. Under the reference period approach, a period of time is selected from the full period of record that is represented by a

data set judged to have good or adequat e spatial and temporal coverage and that appears to support a good trophic state and healthy aquatic life. The reference period sh ould include a time period when the system was fully maintaining i ts designated use, and include nutrient levels and chlorophyll a values representative of natural variability

. The reference period should b e at least 5 consecutive years , preferrably longer (up to 10 years) because Florida is subject to multi

- year drough ts or extreme wet conditions that can affect the magnitude of nutrient loading and attendant water quality in receiving waters. A reference period should sufficiently capture a number of variable rainfall years, but as a whole, not be biased by any extrem e meteorological event of long duration. Because nutrient loadings and concentrations (and presumably Chl a and trophic state) in the TME are partly a function of watershed rainfall

- runoff, the selected reference period
should not be not biased by prolonged drought or wet condition, but should approximate the 30

- year annual average rainfall for the region. A trophic condition was considered desirable for a given segment if the average of trophic state index (TSI) values, as applied to estuaries (FDEP 1996) , was <50.

Chla – [TN] or [TP] R egression M odels

All data from the full period of record were reduced and organized into annual average, average monthly, and
average wet

- season monthly data sets. Simple linear regression models were developed per segment from
these reduced data sets: Chl a levels were r egressed against TN concentrations and separately against TP

concentrations

. If a regression model had a moderate to strong correlation coefficient ( r ) (or coefficient of determination, r 2 ) and significance ( p ) statistic, it was used to predict median concentration limits based on the
reference

- period Chl a medians (the de facto Chl a target levels).

General M odels

Two general nutrient models were applied to the TME to predict limits or acceptable ranges of nutrient loadings and concentrations related to a desirable trophic state (mesotrophy or oligotrophy). One model is an empirically defined relationship between water residence times and nutrient loading limits for mesotrophic Florida systems (Steward and Lowe 2010). Because th is Florida TP trophic loa ding model is consistent with the Vollenweider (1976) TP critical loading model, the Vollenweider model equation was slightly modified (by adjusting the τ w exponent) to fit the Florida systems model (Steward and Lowe 2010) and to predict the upper

or lower mesotrophic [ TP concentration

. The modified Vollenweider equations are as follows:

upper mesotrophic [TP] = L M (P) X τ

w 0.847 /[z(1 + τ

w 0.5 )], which was empirically derived;
lower mesotrophic [TP] = L M (P) X τ

w 0.7 /[z(1 + τ

w 0.5 )] as a theoretical derivation;
whereby L M = mesotrophic loads (g/m 2 /yr); τ

w = water residence time 1 ; z= mean depth (m).

1 Freshwater residence times (=R63 values) were scaled up for TME to R99 values to better represent the longer time for nutrien t detention,, recycling, and trophic utilization of nutrients in lagoons as compared to freshwater systems (Steward and Lowe 2 010 ). A tidal prism volume to freshwater inflow volume ratio was applied in the scaling of freshwater residence times to determine nu trient
limit – trophic status relationship in estuaries. The R99 residence times are 0.27 yr for Tolomato, 0.0377 yr for N. Matanzas, and 0.063 yr for S. Matanzas.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 19 August 2010 The N

- analog of the Vollenweider (1976) model was not equivalent to or consistent with the Florida TN trophic loading model (Steward and Lowe 2010) and, thus, the Vollenweider model could not be used to predict [TN]. Therefore, the Dettmann (2001) model was selected to predict [TN] based on a trophically desirable TN loading limit as determined by prior application of the Florida TN trophic loading model. The Dettman n (2001) model equation is:

[TN] = (L N X τ
w / V + [TN]

s )(1/1 + ατ

w ); whereby L N = TN loading (g/yr), τ

w = freshwater residence time
(yr), V = volume of estuary (liters, L) segment, [TN]
s = average [TN] at segment boundaries (g/L), α = 1 st

order rate coeffi cient for N loss (3.6 yr -1 ).

Among the three lines of evidence, the reference period provides the primary line of evidence because it constitutes the most direct analysis of site

- specific data that represents a time when trophic state is desirable.
The other two lines of evidence are model predictions (incorporating site

- specific data) and regarded as supporting evidence.

Results and Discussion

Spatial C onsiderations and S egmentation

The land use composition among the TME segments, from the Tolomato through S. Matanzas sub

- basins, is similar (Fig ure 9 ) (and similar to the adjacent Halifax R. Estuary catchment; see Steward et al. 2010 b ) with respect to the three major land uses: forest/rangeland, residential/urban, and agriculture. The percent composition of the 3 major land uses in the TME are ~38 – 43% forest/rangeland, ~15

- 28% residential/urban, and ~1% agriculture (SJRWMD 2004 land use, GIS spatial layer).Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 20 August 2010 Figure 9 . Percent composition of major land uses in TME: Tolomato, N. Matanzas, and S. Matanzas (based on 2004 aerial imagery).

Like the Halifax R iver Estuary, spatial differences within the TME may be best described by morphometric and hydrodynamic characteristic s , which significantly mediate the system’s water quality and trophic response. The mean depths of the TM E were significantly increased by the construction of the Intracoastal Waterway (ICW) in the early twentieth century. Among the three segments of the TME, the morphometry of the S. Matanzas estuary is defined largely by the ICW; indeed, much of its southe rn length is the ICW channel.

Water circulation in the TME is driven by strong tidal forces (~1.25 m tidal amplitude). John and Morris (1999) conducted a tracer study with a calibrated RMA2 model (U.S. Army Corps of Engineers) and determine d there

are 3 tidal null or node points within the TME. A tidal node point is a general location within the estuary where there is no net water flow in either direction (north or south). The northernmost point is located near the Palm Valley Bridge (Fig ure 10 a), deli neating the Tolomato’s northern terminus. A second tidal node point is located near the Crescent Beach Bridge (S.R. 206) where the tidal forces funneling through the St. Augustine

and Matanzas inlets counteract (Figure 10b ). The third point is located just south of High Bridge and marks the transition between Matanzas R. and Halifax R. estuaries (Figure 10c ). The tidal node point at Crescent Beach was evaluated in conjunction with water quality data to determine whether this is a reasonable location for a segment boundary. Tidal node points can coincide with points of transition for water quality gradients or biological resources, as observed in the Halifax R. Estuary and Mosquito Lagoon (Steward et al. 2010a, Steward et al. 2010b).

Water residence time is the most important physical factor that determines the trophic response of an estuary to its external nutrient input. The freshwater residence time in the Tolomato R. estuary is about 22 days, which is more than 4 to 5 times longer than in the Matanzas (derived from Sheng et al. 2008). This indicates a nutrient detention time in Tolomato that is considerably longer than in Matanzas and a sensitivity to nutrient enrichment (or a eutrophication susceptibility) that is higher than that for Matanzas with a n equivalent nutrient input (g/m 2 estuary area/yr). 

## Tolomato
(including Guana basin) 

## N. Matanzas

S. Matanzas 0.5%

39.4%

2.0% 11.9%

3.2% 6.1% 36.9%

Agriculture

Forest/Rangeland

Other

Residential

Urban

Water

Wetlands 1.3%

37.9%

1.4% 20.4% 7.7% 3.4% 27.9%

1.3%

42.9%

2.8% 15.2%

2.7% 3.9% 31.2%Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 21 August 2010 Figure 10 a

. Location of tidal node at boundary between the TME and Lower St. Johns R iver E stuary

.

Figure 10b

. Location of tidal node at near Crescent Beach Bridge, midway between St. Augustine Inlet to the
north and Matanzas Inlet to the south. 

### Tidal node

Tidal nodeNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 22 August 2010 Figure 10c

. Location of tidal node at boundary between the Matanzas and Halifax R iver E stuaries.

A two

- segment scheme and a three

- segment scheme was evaluated for the TME based on the longitudinal

gr adients of salinity, TN, TP, Chla, and TSI values (Fig ures 4 - 6).

Mean salinity levels throughout the TME are very uniform, typically >30 ppt as long

- term means except at the very southern and northern ends of the TME (most distant from inlets) where mean salinities are slightly lower, ranging between 27 to 30 ppt. Other than the clear inflection in the salinity gradient between the N. Halifax and S. Matanzas, there is no other apparent inflection point in salinity that would justify segmentation

with in the TME. Nonetheless, there is a small gradient shift in turbidity levels between N. Matanzas and Tolomato, as well as the stronger shift at the High Bridge tidal node point (Fig ure 4).

Unlike salinity and turbidity (Figure 11) , the gradient shifts observed for TN and TP concentrations, Chl a levels, and TSI values are quite distinct ( Figures 12 and 13 ). With respect to those four parameters, two types of gradients are evident and could support dividing the system into 2 or 3 segments depending upon the management question or issue. One gradient perspective that supports a two

- segment scheme is based on a clear inflection point at the Crescent Beach tidal node point where concentrations of nutrient and Chl a and TSI values are generally at their lowest point, and increasing in opposite directions toward the north and south
ends of the TME. The other gr adient perspective is based on a low plateau of TN, TP, and Chl a concentrations and TSI values throughout the N. Matanzas and higher, nearly equivalent plateaus in S. Matanzas and Tolomato. The latter gradient perspective supports a three

- segment scheme that is preferred for nutrient criteria development because there is tighter consistency in water quality within each of the three segments, potentially yielding more definitive criteria (subject to less intra

- segment variability) than the two

- segment
schem e.

Stringent non

- parametric analyses indicate significant differences among the three segments for TN and TSI; and significant differences between N. Matanzas and the other two segments for turbidity, Chl a , and TP (Kruskall

- Wallis; family α = 0.05 for the 3 segments). 

## Tidal node

Bulow Cr.



### Google EarthNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 23 August 2010 Figure 11. Spatial distribution of salinity and turbidity (mean and st d. d eviation) by sampling site with median levels by segment included (1986

– 2009 period of record). The SEAS/FDACS sites are included only in the salinity graph; both graphs include the SJRWMD and GTMNERR sites. 0 5 10 15 20 25 30 35 40Salinity (ppt)

Site 

### Salinity

Dry Wet High Bridge St. Augustine Inlet Matanzas Inlet

Tolomato

median: 31.9 ppt 

### 1986

– 2009 period of record

N. Matanzas
median: 33.5 ppt S. Matanzas
median: 31.5 ppt

0 2 4 6 8 10 12 14 16 18Turbidity (ntu)

Site 

### Turbidity Dry Wet

High Bridge Matanzas Inlet St. Augustine
Inlet Palm Valley Bridge S. Matanzas
median: 7.8 ntu N. Matanzas
median: 7.5 ntu Tolomato

median: 9.4 ntu Tidal

node pointNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 24 August 2010 Figure 12

. Spatial distribution of TN, TP, and Chla (mean ± std deviation) by SJRWMD or GTMNERR sampling site with segment mean and median included (1986

–

2009 period of record). 0 2 4 6 8 10 12 14 16CHLAC μg/l)

Site Chlorophyll a (corrected) Dry Wet 0 0.05 0.1 0.15 0.2 0.25 0.3TP (mg/l)

Site Total Phosphorus Dry Wet

High Bridge 0 0.2 0.4 0.6 0.8

1 1.2 1.4 1.6 1.8

2TN (mg/l)

Site Total Nitrogen DRY Wet

Palm Valley Bridge Matanzas Inlet St. Augustine
Inlet High Bridge 

### S. Matanzas median: 0.49 mg/L mean: 0.50 mg/L 

### N. Matanzas

median: 0.41 mg/L

mean: 0.43 mg/L 

### Tolomato
median: 0.53 mg/L mean: 0.58 mg/L



### S. Matanzas median: 0.083 mg/L mean: 0.10 mg/L 

### N. Matanzas median: 0.072 mg/L mean: 0.087 mg/L 

### Tolomato
median: 0.084 mg/L mean: 0.098 mg/L



### S. Matanzas

median: 3.9 mg/L mean: 4.6 mg/L 

### N. Matanzas

median: 3.1 mg/L mean: 3.4 mg/L 

### Tolomato

median: 4.3 mg/L

mean: 5.0 mg/L Mean ± s.d

.

Mean ± s.d

.

Mean ± s.d

. Tidal

node pointNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 25 August 2010 Figure 13

. Spatial distr ibution of TSI values by SJRWMD or GTMNERR sampling site with segment mean and median included (1986

–

2009 period of record).

The lowest trophic state index (TSI) values 2

The information above regarding the spatial distribution of physical properties and trophic water quality support a recommendation for dividing the TME into 3 segments – Tolomato, N. Matanzas, and S. Matanzas

-- for the development of segment

- specific nutrient criteria. occur in the N. Matanzas segment, reflecting the segment’s low Chla and nutrient levels. The short water residence time (oceanic flushing via St. Augustine and Matanzas inlets) is the likely reason for this spatial trend.

Temporal C on siderations and the R eference P eriod M ethod

Time series graphs for TN, TP, Chl a , and TSI values are shown in Figures 14 -17 , comparing two long

- term (quasi

- decadal) periods, 1986 – 1999 and 2000 – 2009. Differences between the periods are slight or

modera te, albeit statistically significant (Kruskall

- Wallis; α = 0.05; seasonal Kendall trend analysis, p ≤ 0.03). For the N. and S. Matanzas segments, TP, Chl a , and TSI levels increased slightly, while TN levels decreased slightly from the early to the latter p eriod. For the Tolomato segment, TP and Chl a increased and TN decreased from the early to lat t er period, while TSI exhibited no trend.

It is important to note that a strong temporal bias exists in the 1986 – 1999 record in which 64 – 84% of the
data were collected in 1997 – 1999 , which included an extended drought period (1998 – 2000).Th is
unintentional sampling bias is largely attributable to a long over

- due expansion of the TME monitoring

program that began in 1997. Sampling sites were added (one SJR WMD site per segment was added in 1997

2 TSI values were calculated using the equation specified in FDEP (1996) and the value ranges for estuaries, as specified by FD EP (1996),

are 0 – 49 good; 50 – 59 fair; and 60 – 100 poor. 0 10 20 30 40 50 60

HL01 JXTR26 MAT MRT GTMFM JXTR21 MR312 JXTR17 GTMPI TOLTSI

Site 

# TSI

High Bridge St. Augustine Inlet Matanzas Inlet Mean + STDEV

Palm Valley Bridge


### S. Matanzas

median: 38.7

mean: 38.6 

### N. Matanzas

median: 36.3

mean: 35.2 

### Tolomato

median: 41.9

mean: 41.2 

### Trophic State IndexNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 26 August 2010 and two GTMNERR sites were added in 2001) and sampling frequency increased from quarterly to monthly. Consequently, there are substantially more data in the 2000 – 2009 record than in the 1986 – 1999 record. In
add ition, the 1986 – 1999 record is discontinuous; sampling was suspended at site MRT in S. Matanzas for 7 years (1990 through 1996); and throughout the TME, the 1986 – 1999 record is heavily weighted in its last 3 years (1997 – 1999) when sampling frequency tripled and the extended drought occurred. However, even with the removal of the drought data, TP trended upward throughout the TME, slightly but significantly (seasonal Kendall, p ≤ 0.002), during the full, two

- decade period of record. The rate of TP increase is approximately 0.001 mg/L per year based on the slope of the trend line (with or without drought data). S. Matanzas is the only segment in the TME where Chl a and TSI may have responded to that TP incline (seasonal Kendall, p < 0.02 excluding drou ght data, p< 0.0001 all data) with annual rates of increase of ~0.06 μg/L and 0.16 units, respectively.

Compared to the 1986 – 1999 record, the - 2009 record is more temporally and spatially complete , and subject to less bias or influence by episodic event s like droughts. Therefore, the 2000 – 2009 record appears to be more representative of long

- term water quality conditions throughout the TME. The median or mean Chl a values for the 2000 – 2009 period are still indicative of good trophic quality as existed in the prior decade

. A verage annual Chl a levels in all segments were below the target levels set for Florida’s estuaries (typically ≤7 μg/L annual avg. ) proposed by Steward and Lowe 2010). Moreover, the average annual rainfall during 2000 – 2 009 was close to normal (49”/yr as a basin

- wide average) with no extended drought that would have a dampening effect on decadal medians and means. For those reasons, 2000 – 2009 is proposed as the reference period for development of TME nutrient criteria. Even though S. Matanzas’ trophic state may have slightly increased over the last 10 years, its current trophic state is good (TSI = 39) and certainly on par with the Tolomato segment (TSI = 42), and application of the proposed reference period to criteri a development should promote maintenance of that trophic state.

0 0.2 0.4 0.6 0.8 1 1.2 1.4TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 1 0 0.2 0.4 0.6 0.8 1 1.2 1.4TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 2

0 0.5 1 1.5 2 2.5TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 3 S. Matanzas N. Matanzas

Tolomato Times series graphs of annual mean [TN] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0.53 mg/L mean

0.50 mg/L median 0.49 mg/L mean

0.45 mg/L median 0.50 mg/L mean

0.50 mg/L median 0.41 mg/L mean

0.37 mg/L median

0.62 mg/L mean

0.53 mg/L median 0.56 mg/L mean

0.52 mg/L median Figure 14.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 27 August 2010 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 2

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 3 S. Matanzas N. Matanzas

0.09 mg/L mean

0.069 mg/L median 0.10 mg/L mean

0.089 mg/L median 0.10 mg/L mean

0.068 mg/L median 0.083 mg/L mean

0.073 mg/L median

0.10 mg/L mean

0.084 mg/L median 0.10 mg/L mean

0.085 mg/L median Tolomato Times series graphs of annual mean [ TP] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 2

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 3 S. Matanzas N. Matanzas

0.09 mg/L mean

0.069 mg/L median 0.10 mg/L mean

0.089 mg/L median 0.10 mg/L mean

0.068 mg/L median 0.083 mg/L mean

0.073 mg/L median

0.10 mg/L mean

0.084 mg/L median 0.10 mg/L mean

0.085 mg/L median Tolomato Times series graphs of annual mean [ TP] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

Figure 15.

Figure 16.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 28 August 2010 0 2 4 6 8 10 12 14 16 18 20CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 1 0 2 4 6 8 10 12CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 2

0 5 10 15 20 25 30CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 3 S. Matanzas N. Matanzas

3.8 μg/L mean

2.9 μg/L median 5.0 μg/L mean

4.3 μg/L median 3.4 μg/L mean

2.7 μg/L median 3.5 μg/L mean

3.1 μg/L median

4.4 μg/L mean

3.2 μg/L median 5.3 μg/L mean

4.5 μg/L median Tolomato Times series graphs of annual mean [ Chl a ] ± Std dev

, dry and wet season, in the S. Matanzas, N. Matanzas
and Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 1 0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 2

0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 3 Times series graphs of annual mean Trophic State Index (TSI) values ± Std dev, dry and wet season, in the

S. Matanzas, N. Matanzas and Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

36.4 mean
37.4 median 39.6 mean
39.3 median 34.5 mean
35.7 median 35.4 mean

36.4 median

39.5 mean
41.2 median 41.8 mean
42.2 median Figure 17.

Figure 18.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 29 August 2010 Figure 19 . Time series graphs of annual mean [TN] ± Std dev

, d ry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

Note: Long

- term (decadal) mean and median concentration values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term med ians.

0 0.2 0.4 0.6 0.8 1 1.2 1.4TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 1 0 0.2 0.4 0.6 0.8 1 1.2 1.4TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 2

0 0.5 1 1.5 2 2.5TN (mg/l)

Year Total Nitrogen Dry Wet

Segment 3 S. Matanzas N. Matanzas

Tolomato Times series graphs of annual mean [TN] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0.53 mg/L mean

0.50 mg/L median 0.49 mg/L mean

0.45 mg/L median 0.50 mg/L mean

0.50 mg/L median 0.41 mg/L mean

0.37 mg/L median

0.62 mg/L mean

0.53 mg/L median 0.56 mg/L mean

0.52 mg/L medianNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 30 August 2010 Figure 20 . Time series graphs of annual mean [TP] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

Note: Long

- term (decadal) mean and median concentration values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 2

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 3 S. Matanzas N. Matanzas

0.09 mg/L mean

0.069 mg/L median 0.10 mg/L mean

0.089 mg/L median 0.10 mg/L mean

0.068 mg/L median 0.083 mg/L mean

0.073 mg/L median

0.10 mg/L mean

0.084 mg/L median 0.10 mg/L mean

0.085 mg/L median Tolomato Times series graphs of annual mean [ TP] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 31 August 2010 Figure 21 . Time series graphs of annual mean [TP] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

Note: Long

- term (decadal) mean and median concentration values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 2

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9TP (mg/l)

Year Total Phosphorus Dry Wet

Segment 3 S. Matanzas N. Matanzas

0.09 mg/L mean

0.069 mg/L median 0.10 mg/L mean

0.089 mg/L median 0.10 mg/L mean

0.068 mg/L median 0.083 mg/L mean

0.073 mg/L median

0.10 mg/L mean

0.084 mg/L median 0.10 mg/L mean

0.085 mg/L median Tolomato Times series graphs of annual mean [ TP] ± Std dev , dry and wet season, in the S. Matanzas, N. Matanzas and
Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 32 August 2010 Figure 22

. Time series graphs of annual mean [Chla] ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, and Tolomato segments.

Note: Long

- term (decadal) mean and median concentration values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

0 2 4 6 8 10 12 14 16 18 20CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 1 0 2 4 6 8 10 12CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 2

0 5 10 15 20 25 30CHLAC (μg/l)

Year Chl a corrected Dry Wet

Segment 3 S. Matanzas N. Matanzas

3.8 μg/L mean

2.9 μg/L median 5.0 μg/L mean

4.3 μg/L median 3.4 μg/L mean

2.7 μg/L median 3.5 μg/L mean

3.1 μg/L median

4.4 μg/L mean

3.2 μg/L median 5.3 μg/L mean

4.5 μg/L median Tolomato Times series graphs of annual mean [ Chl a ] ± Std dev

, dry and wet season, in the S. Matanzas, N. Matanzas
and Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 33 August 2010 Figure 23 . Time series graphs of annual mean Trophic State Index (TSI) values ± Std dev, dry and wet season, in the S. Matanzas, N. Matanzas, an d Tolomato segments.

Note: Long

- term (decadal) mean and median concentration values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

Reference

- period results for Chl a , TN, and TP (Table s 8 a and 8b) are annual median concentrations and

maximum wet

- season median concentrations based on the highest monthly values, which are typically

measured in June – September (mean annual and wet

- season values are also provided in Table s 8 a and 8b ).
The reference

- period results constitute the first and primary line of evidence in the development of nutrient

criteria for the TME. Selection of the 2000 – 2009 as a reference period for TME is consistent wi th the reference periods selected for Halifax R. Estuary (2000 – 2008) and Mosquito Lagoon (2004 – 2008) (Steward et al. 2010a; 2010b ).

Table 8a

. Tolomato

- Matanzas Estuary, r eference p eriod r esults –1 st line of evidence : Annual median (mean),

2000

– 09

.

**Se gment**  **Chla (μg/L) TN (mg/L)**  **TP (mg/L)**

Tolomato 4.5 (5.3) 0.52 (0.56) 0.085 (0.096)

N. Matanzas 3.1 (3.5) 0.37 (0.41) 0.073 (0.083)

S. Matanzas 4.3 (5.0) 0.45 (0.49) 0.089 (0.103)

0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 1 0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 2

0 10 20 30 40 50 60TSI

Year TSI Mean + STDEV

Segment 3 Times series graphs of annual mean Trophic State Index (TSI) values ± Std dev, dry and wet season, in the

S. Matanzas, N. Matanzas and Tolomato segments.

Long

- term (decadal) mean and median concentration
values for the 1986

– 99 and 2000

– 09 periods are included. The record for N. Matanzas only extends back to 1991. The bold horizontal lines represent the long

- term medians.

36.4 mean
37.4 median 39.6 mean
39.3 median 34.5 mean
35.7 median 35.4 mean

36.4 median

39.5 mean
41.2 median 41.8 mean
42.2 medianNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 34 August 2010 Table 8b. Tolomato

- Matanzas Estuary, reference period results

– 1st line of evidence: Maximum wet

- season median (mean) (June

– September)

. **Segment Chla (μg/L)**  **TN (mg/L) TP (mg/L)** 

Tolomato 6.4 (7.4) 0.61 (0.65) 0.095 (0.105)

N. Matanzas 4.0 (4.4) 0.42 (0.46) 0.074 (0.085)

S. Matanzas 6.3 (7.2) 0.54 (0.62) 0.112 (0.127)

The maximum wet

- season medians for Chl a in the TME (4.0 – 6.4 μg/L; Table 8 ) are relatively low in terms of the levels normally associated with harmful algal blooms, or HABs (e.g., ≥12 μg/L in the Halifax R. Estuary; analysis of Dr. Edward Phlips, U.F., 2000 – 2004 data set for S. Matanzas southward to northern Mosquito Lagoon). The HAB species encountered in the TME include the potentially toxic dinoflagellates Karlodinium veneficum, Cochlodinium polykrikoides, Prorocentrum minimum , and Takayama spp. , the microflagellate Chrysochromulina sp. , and the diatom Pseudo

- nitzschia cal liantha

. Not one of these species was observed in bloom proportions during the TME samplings (Edward Phlips, U.F., pers. com., July 2010). Compliance with the
median or mean concentrations for Chl a , TN, and TP, especially the wet season levels (Table 8), will not eliminate these HAB species, but should restrict their concentrations below bloom levels.

Chla

– [TN ]

, [– [TN, TP ] R egression M odels

Covariance among average monthly TN, TP, and Chl a was apparent (Figure 19 ) and prompted development of simple re gre ssion models

.

Figure 24

. Average monthly [TN], [Chl a], and [TP] in the TME overall. Monthly rainfall data are shown for
comparison. The 1986 – 2009 period was used for the average monthly water quality data; and the 1986 – 2008 period was used for a verage monthly rainfall. TP *10 CHLAC TN * 10

0 1 2 3 4 5 6 7

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Average Monthly Nutrients, Chl a , and Rainfall

TP *10 CHLAC TN * 10

3.1" 3.0" 6.1" 4.8 " 5.8" 2.3" 2.2" 3.9" 2.4" 2.4" 4.9" 7.5"

avg. monthly rainfall based on average of measured rainfall at Jacksonville Beach, St. Augustine, Marineland, and Daytona MonthNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 35 August 2010 If any of the regressions proved statistically significant, they were applied toward setting median TN and TP based on Chla “targets” pre

- determined by the reference

- period method (see Table 8 ). Annual average and

wet

- season monthly average regressions are not significant and, thus, not used to predict annual and wet

season median concentrations. However, the monthly average regressions based on all months are significant with moderately stro ng correlation and determinant coefficients, and are used to predict maximum monthly,

wet

- season median concentrations (no annual medians or means were predicted). Results based on those linear regressions are the second line of evidence specific to wet -s eason criteria (Table 9 ).

Table 9. Tolomato

- Matanzas Estuary, linear regression results –2 nd line of evidence: Maximum wet

- season median (1986

– 2009; all months).

**Segment Target Chla**  **(μg/L) TN**  **(mg/L) r**  **p TP**  **(mg/L) r**  **p**

Tolomato 6.4 0.62 0.63 .028 0.110 0.68 .014

N. Matanzas 4.0 0.46 0.72 .008 0.095 0.81 .002

S. Matanzas 6.3 0.61 0.87 .0002 0.120 0.82 .001Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 36 August 2010 Figure 19

. Average monthly [TN], [Chl a], and [TP] in the TME overall. Monthly rainfall data are shown for comparison. The 1986 – 2009 period was used for the average monthly water quality data; and the 1986 – 2008 period was used for average monthly rainfall.

The strongest Chl a – nutrient regressions were produc ed for the S. Matanzas segment

, followed by the N.

Matanzas segment

. segments

. TN:TP ratios are generally <7 (by mass) among the segments indicative of a system that is N

- limited, but at times may tend toward a N+P, co

- limited state 3

Whether or not a relationship between nutrients and Chl a exists does not necessarily reflect the system’s

nutrie nt or trophic state (Borum 1996, Cloern 1991). Even if a system is nutrient rich, its trophic response could be limited by other factors. Given that the TME system has relatively low Chl a and trophic state, it is likely that a combination of factors controlling Chl a concentrations may be involved, including residence t ime, water temperature, nutrients, and possibly tripton (nonalgal turbidity). in the Tolomato segment. Dix and Phlips (2010) and Phlips et al. (2004) found phytoplankton production (Chl a ) in the TME was subject to N

limitation primarily, followed by N+P as a secondary limitation. In contrast to the regression analysis (Table 9 ), Phlips et al. (2004) found that Chl a was not correlated to either TN or TP, but that a positive correlation
between Chl a and water temperature did exist, reflecting peak production during the warm months of late spring into early fall (similar to the trend observed in Fig. 11). They concluded that phytoplankton production is regulated more by physical factors, water residence times and temperature, than by nutrients.

Turbidities are relatively high in the TME, particularly in comparison to the Indian River Lagoon (IRL). TME turbidities reached levels that were roughly twic e that observed i n the IRL (Figure 20), and were nearly

3 TN:TP ratio, by mass, <7 suggests N limitation; ~7.2 suggests a nutrient balance state (Redfield et al. 1963; Hecky et al. 1993). TP *10 CHLAC TN * 10

0 1 2 3 4 5 6 7

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Average Monthly Nutrients, Chl a , and Rainfall

TP *10 CHLAC TN * 10

3.1" 3.0" 6.1" 4.8 " 5.8" 2.3" 2.2" 3.9" 2.4" 2.4" 4.9" 7.5"

avg. monthly rainfall based on average of measured rainfall at Jacksonville Beach, St. Augustine, Marineland, and Daytona MonthNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 37 August 2010 consistent with levels in the South Halifax R. Estuary (S. HRE). The turbidity levels in the TME may be a natural condition, or close to it. The TME is similar to many well

- flushed, yet naturally turbid estuaries in the South Atlantic Bight region (NE Florida through South Carolina) that are dominated ecologically by oyster and marsh communities. Turbidity or tripton is one factor (short water residence time, appreciable tidal amplitude and tidal current being other factors) that constrains phytoplankton and submersed macrophyte production in favor of marsh production in the estuaries of northeast Florida, Georgia, and South Carolina (Lee and Jones 1981; Dame et al. 2000; Roman et al. 2000; Robert Virnstein, Seagrass Ecosystems Analyst, pers. com., April 2010; Edward Phlips, U.F., pers. com., April 2010).

Figure 20

. Mean turbidity by year (1992

– 2008) for TME in comparison to the North and South Halifax R iver Estuary (N. HRE and S. HRE) and the Indian River Lagoon (IRL).

General M odels

Both models (Steward and Lowe 2010; Dettmann 2001) require estimates of water residence time and external nutrient loadings to predict trophic state, loading limits, and complementary concentrations of TN and TP. Estimates of external nutrient loadings for the Tolomato, N. Matanzas, and S. Matanzas are presented in
Table 10.

Nonpoint surface water drainage conveys the largest percentage of the total external nutrient loading t o TME overall (68% for TN, and 55% for TP), followed by the wastewater treatment facilities (WWTFs; 24.5% for TN,

and 37% for TP). The N. Matanzas segment is a notable exception to the basin

- wide trend. N. Matanzas WWTF TN loading is approaching that of surface water drainage, and WWTF TP loading exceeds surface

- water drainage TP loading (Table 10 ).

Atmospheric loading estimates, based on areal loading estimates for Northeast Florida (Pollman and Roy 2003), may constitute ~5% of the total TN loading and <1% of total TP loading. TME

- specific measurements of groundwater loading are not available; instead, estimated groundwater loading rates derived from a study conducted in the adjacent basin of the Lower St. Johns River basin (Ouyang 2005) were used to estimate TME groundwater loadings. The geohydrology (e.g., thicknesses of surficial aquifer and underlying confining layer) of the St. Johns River study area is not appreciably different from that of the TME basin (Y. Ouyang and D. Toth, SJRWMD, pers. com m., July 2010). 0 2 4 6 8 10 12 14 16 18Turbidity (ntu)

Year 

### Mean Turbidity in N. HRE, S. HRE, TME, and IRL

Mean Turb.

- N. HRE Mean Turb.

- S.HRE Mean Turb.

- GTM Mean Turb.

- IRLNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 38 August 2010 Table 10. Estimates of current external loadings of TN and TP to Tolomato

- Matanzas Estuary (2004 land use,

2001

– 2008 avg. annual rainfall of 48”)

.

- = Empty cell/no data

a SJRWMD PLSM - estimated surface

- water loadings based on 2004 land us e and 2001

- 2008 avg. annual rainfall, which is close to the 30

- yr avg. annual
rainfall of 51”. b Atmospheric loading estimates were derived from a study applicable to the NE Florida coastal region (Duval and St. Johns coun ties) by Pollman and Roy (2003). c Domestic WWTFs: Marsh Landing in Tolomato; SR16 St. Johns County, Anastasia St. Johns County, and City of St. Augustine in N. Matanzas; Palm Coast, Beverly Beach, and Flagler Beach in S. Matanzas. WWTF annual loading estimates per facility were pr ovided by FDEP, Northeast District, Jacksonville. d Groundwater estimates derived from study in Duval County, Lower St. Johns R. basin, due west of the Tolomato watershed, conducted by Ouyang (2005). We are not aware of any groundwater nutrient loading study condu cted in the TME basin.
**Table 10.** Estimates of current external loadings of TN and TP to
Tolomato

- Matanzas Estuary ( 2004 land use, 2001

– 2008 avg. annual rainfall of 48”) **Sources TN (kg/yr)**  **TP (kg/yr)**

Tolomato Sources TN (kg/yr)
- TP (kg/yr)
- Surface water drainagea 63,353 8,609 Atmosphericb 8,350 161

WWTFc 724 295295
Groundwaterd 4,300 2,200 Total Loading: 76,727 (5.96 g/m2/yr) 11,264 (0.82 g/m2/yr)

North Matanzas Sources TN (kg/yr)
- TP (kg/yr)
- Surface water drainagea 120,981 18,585
Atmosphericb 10,607 204

WWTFc 101,186 22,803

Groundwaterd 4,300 2,200 Total Loading: 237,074 (13.53 g/m2/yr) 43,792 (2.5 g/m2/yr)

South Matanzas Sources TN (kg/yr)
- TP (kg/yr)
- Surface water drainagea 170,557 23,474 Atmosphericb 7,362 142

WWTFc 25,806 11,326
Groundwaterd 4,300 2,200 Total Loading: 208,025 (17.10 g/m2/yr) 37,142 (3.05 g/m2/yr)

The total external TN and TP loading estimates ( ca 2004 loadings) for the TME segments were normalized to estuarine surface water area (g/m 2 /yr) for input to the Steward and Lowe (2010) model. These areal loading estimates for each segment were then paired with their respective hydraulic loadings (mean depth/residence time) and plotted on a graph that describes TN or TP areal loading limit (p ublished TMDLs or PLRGs) as a function of estuarine hydraulic loading (Fig. 13). The line of best fit in the graph represents the upper mesotrophic boundary line (UML) for Florida estuaries (based on Steward and Lowe 2010). The line in the plot representi ng lower mesotrophy (LML) was constructed using [TN] and [TP] limits for lower mesotrophy established by Steward and Lowe (2010), Smith (1998), and Baker et al. (1981) (further explanation provided in Steward and Lowe 2010).

The plotted points for TN and TP are located between the UML and LML for Tolomato and S. Matanzas segment and just below the LML for N. Matanzas, indicating lower mesotrophy or oligo

- mesotrophy (Fig ure
21 ). The TSI values for the TME also indicate mesotrophy with N. Matanzas exhibiting the lowest trophic state among the 3 segments. If the trophic positions of the points in the graphs are deemed suitable as a basis for nutrient criteria, then the current TN and TP loadings can be considered the loading limits.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 39 August 2010 Figure 21. The log

- linear plot of TN and TP load limits (g/m 2 /yr) vs. hydraulic loading (q

s ) adapted and improved from Steward and Lowe (2010) by the incorporation of additional data points from mesotrophic estuaries initially used to validate the models. A point lying above the 95% confidence interval for the line

of

- best

- fit (=upper mestrophic line or UML) is considered eutrophic; below the lower mesotrophic line ( LML) is considered oligotrophic

.

Finally, the Dettmann (2001) and Steward and Lowe (2010) models were used to predict [TN] and [TP] in the TME segments that correspond to the estimated current loadings ( ca

. 2004 loadings) , which could be considered the system’s external loading limits . The TN and TP loading limits and their predicted concentrations are presented as the 3 rd line of evidence (Table 11 ).

Table 11

. Tolomato

- Matanzas Estuary loading limits ( ca . 2004 loadings) for TN and TP and their

model

- predicted concentrations **Segment** TN: kg/yr

— mg/L TP: kg/yr

— mg/L

Tolomato segment TN: kg/yr mg/L
- TP: kg/yr mg/L
- Loading Limit & l imit and predicted

concentration 76,727

— 0.48 * 11,264

— 0.060 **

N. Matanzas segment - - Loading Limit & l imit and predicted 237,074

— 0.38* 43,792

— 0.073*** 

### S. Matanzas

Tolomato

- 0.5 0.5

1 1.5

2 2.5

- 0.5 0 0.5 1 1.5 2 2.5 3


### log q s 

### (z/ τ w 

### ; m/yr)log TN load limit (g/m2/yr) 95% upper CI

LML



### N. Matanzas

- 2.0 - 1.5 - 1.0 - 0.5 0.0 0.5 1.0 1.5

- 0.5 0.0 0.5 1.0 2.0 2.5 3.0



### log q s 

### (z/ τ w 

### ; m/yr)log TP load limit (g/m2/yr)



### N. Matanzas S. Matanzas

Tolomato 2.0 95% upper CI

LML 

## TN - log TN Load Limit ( ≈UML) = 0.83(log q

s ) – 0.018; R 2 = 0.96, p < 0.001



## TP

- log T P Load Li mit ( ≈UML) = 0. 92 (log q
s ) – 0.95; R 2 = 0.97, p < 0.001Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 40 August 2010 concentration S. Matanzas segment - - Loading Limit & l imit and predicted

concentration 208,025

— 0.45* 37,142

— 0.109 €



### Summary and Conclusions

Results from the three lines of evidence are tabulated for ease of comparison (Table 12 ). Overall, the
results from the TME

- specific regression models and the general models compare well with the
reference

- period results, increasing the level of confidenc e that numeric nutrient criteria for the TME
can be based on the 2000

- 2009 period of record. However, the TP general model result (0.060 mg/L annual median or mean) for the Tolomato does not compare well with the reference

- period results (0.086 mg/L median, 0.096 mg/L mean). This inconsistency cannot be explained, but it is the exception in an otherwise solid, weight

- of

- evidence case for the TME system.

The Chla and trophic response s of the TME segments appear to be affected by water residence time, nutrient loading, and possibly tripton. Nutrient loading is the one factor that can be controlled to manage the estuary’s trophic response; the other two factors are largely natural factors that can not be
controlled. O ngoing efforts to implement extra

- regulatory stormwater treatment, promulgate basin

specific stormwater criteria (e.g., Outstanding Florida Waters designation by the state and/or Special Basin Designation by SJRWMD), and a cquire natural lands for conservation (Haydt and Frazel 2003) will partially offset future nutrient loading increases

. The addition of numeric nutrient criteria can help ensure protection of the current trophic state. Toward that end, the recommended nut rient criteria are based on the reference

- period concentration results and the current external loading rates, which can be rounded up to the nearest 10,000 kg/yr for TN and to the nearest 1,000 for TP (e.g., Tolomato TN loading limit of 76,727 kg/yr round ed up to 80,000 kg/yr; see Table 12.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 41 August 2010 Table 12 a . Summary of lines

- of

- evidence results: loading and concentration limits for the Tolomato
segment

.

Tolomato: mesotrophy (Chla targets: 4.5 μg/L annual median; 6.4 μg/L Jun – Sep median)

- = Empty cell/no data **Loading Limit (current loading)**  **TN (kg/yr) 76,727** 

**– 80,000**  **TN (kg/yr) 76,727** 

**– 80,000**  **TP (kg/yr) 11,264** 

**– 12,000**  **TP (kg/yr) 11,264** 

**– 12,000** 

- [TN] median (mean) (mg/L)

Annual [TN] median (mean) (mg/L)

June

- September [TP] median (mean) (mg/L)

Annual [TP] median (mean) (mg/L)

June

- September
Reference Period (’00

- 09 period) 0.52 (0.56) 0.61 (0.65) 0.085 (0.096) 0.095 (0.105)

Regressions (’86

- 09 data) N ot sign ificant 0.62 N ot sign ificant 0.110

General Models 0.48 Not applicable 0.060 Not applicable

Table 12 b . Summary of lines

- of

- evidence results: loading and concentration limits for the North Matanzas segment.

N. Matanzas: oligo

- mesotrophy (Chla targets: 3.1 μg/L annual median; 4.0 μg/L Jun – Sep median)

- = Empty cell/no data **Loading Limit (current loading)**  **TN (kg/yr) 237,074** 

**– 240,000**  **TN (kg/yr) 237,074** 

**– 240,000**  **TP (kg/yr) 43,792** 

**– 44,000**  **TP (kg/yr) 43,792** 

**– 44,000** 

- [TN] median (mean) (mg/L)

Annual [TN] median (mean) (mg/L)

June

- September [TP] median (mean) (mg/L)

Annual [TP] median (mean) (mg/L)

June

- September
Reference Period (’00

- 09 period) 0.37 (0.41) 0.42 (0.46) 0.073 (0.083) 0.074 (0.085)

Regressions (’86

- 09 data) N ot sign ificant 0.46 N ot sign ificant 0.095

General Models 0.38 Not applicable 0.073 Not applicable

Table 12 c . Summary of lines

- of

- evidence results: loading and concentration limits for the South Matanzas segment.

S. Matanzas: mesotrophy (Chla targets: 4.3 μg/L annual median; 6.3 μg/L Jun – Sep median)

- = Empty cell/no data **Loading Limit (current loading)**  **TN (kg/yr) 208,025** 

**– 210,000**  **TN (kg/yr) 208,025** 

**– 210,000**  **TP (kg/yr) 37,142** 

**– 40,000**  **TP (kg/yr) 37,142** 

**– 40,000** 

- [TN] median (mean) (mg/L)

Annual [TN] median (mean) (mg/L)

June

- September [TP] median (mean) (mg/L)

Annual [TP] median (mean) (mg/L)

June

- September
Reference Period (’00

- 09 period) 0.45 (0.49) 0.54 (0.62) 0.089 (0.103) 0.112 (0.127)

Regressions (’86

- 09 data) N ot sign ificant 0.61 N ot sign ificant 0.120

General Models 0.45 Not applicable 0.109 Not applicable

.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 42 August 2010 Proposed Numeric Nutrient Targets

Methodology

For consistent application and for providing an appropriate level of protection, water quality criteria
need to include magnitude, frequency, and duration components. The magnitude is a measure of how
much of a pollutant may be present in the water without an unacceptable adverse effect. Duration is a
measure of how long the pollutant may be above the magnitude, and frequency relates to how often
the magnitude may be exceeded without adverse effects. It is preferred to derive the magnitude

c omponent of a criterion through a cause

- effect relationship (such as those measured through toxicity
testing). The magnitude would then be set at a level that would protect a majority of the sensitive
aquatic organisms inhabiting the system. Absent a demonstrated cause

- effect relationship, the
magnitude may be set at a level designed to maintain the current data distribution, accounting for
natural temporal variability, when the current conditions are protective of the designated uses of the
waterbody. Since a criterion derived based on the existing data distribution has no direct link to any
observed cause and effect relationship, it is assumed that maintaining the current data distribution will
preserve the uses associated with that distribution.

The frequency and duration components of the criteria are best established as additional descriptors of

the reference condition data distribution. Specifically, these components should be part of a statistical
test designed to determine whether the long

- term distribution of data has shifted upward ( or in some
cases, downward) from the reference distribution. This test would then be used to determine whether
future monitoring data are consistent with the magnitude (long

- term average) defined by the reference

dataset. It is critical to account for the natural variability surrounding the magnitude expression and to

control for statistical errors.

The derivation of the magnitude, frequency and duration components of the numeric nutrient criteria
for TME is described briefly below. More details concerning the statistical approaches used can be
found in the document “Overview of FDEP Approaches for Nutrient Criteria Development in Marine
Waters” (FDEP 2010).

Magnitude

The magnitude component can be set at a variety of values based on the frequency and duration that
will be used to determine compliance. For the “healthy existing conditions” approach, FDEP established
the magnitude at: a) an adjusted long

- term central tendency (geometric mean) of the distributi on, expressed as a long

- term geometric mean never to be exceeded; and b) an annual geometric mean, not

to be exceeded more than two times over a five - year period. The long

- term average condition was
adjusted to a level that represents an insignificant in crease from the baseline. Allowing for an
insignificant increase accounts for uncertainty in the estimate of the mean and partially addresses the
fact that the reference based approach fails to identify a threshold of imbalance, and thus is highly

prone t o excessive Type I error. For the purpose of antidegradation evaluations, EPA has established
that a ten percent change in a water quality parameter represents an insignificant departure from the
historical condition, known as a de minimus change (US Sixt h Circuit Court 2008). Therefore, the long

-Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 43 August 2010 term geometric mean limit was derived to allow for up to a ten percent increase in the long

- term geometric mean, which was calculated as the geometric mean of the annual geometric means of the
long

- term dataset f or each segment of the waterbody.

Because it is not always practical to assess compliance with the criteria on the same long

- term basis,
FDEP also developed criteria expressed as an annual geometric mean so that estuaries could be assessed

on an annual bas is. It should be noted that the duration and frequency components of the criteria must
be linked to the response timeframe of the sensitive endpoint. Short

- term averaging periods (e.g., 1 to
30 days) might be appropriate for nutrient criteria where a suf ficiently robust cause

- effect relationship
has demonstrated that a eutrophic response occurs over such timeframes. If however, such a short

term response cannot be demonstrated, or there is no indication of use impairment, longer averaging
periods should be used.

Since the duration and frequency components of the criteria must be consistent with the derivation of
the magnitude component, the long

- term geometric mean target cannot simply be applied as an annual

mean. Doing so would result in an unaccept ability high Type I failure rate (identifying a healthy system
as being impaired) since approximately 50 percent of the individual yearly means are expected to be
above the long

- term mean. Therefore, the long

- term target must be adjusted to allow for the
application to a shorter duration with an acceptable Type I error rate of no more than a 10 percent.

An annual criterion with an approximate 10 percent Type I error rate for a given frequency was derived
by appropriately accounting for the annual variabili ty above the mean. This annual target concentration
was derived as an upper percentile of the distribution of the annual geometric mean concentrations.
Previous proposals by EPA have utilized three

- year assessment periods to express the duration nutrient
criteria components. Although it is possible to construct a test that achieves the 10 percent Type I error
rate target over a 3

- year period, a slightly longer period (5 years) will provide better control for Type II
error and will more fully capture cli matic cycles ( e.g ., el nino, la nina), which tend to be longer than
three years in Florida (and may actually be on a multi

- decadal scale). Furthermore, a five year period is
more consistent with both the State’s five year 303(d) assessment and NPDES permit renewal cycles.

Given a five year assessment period, FDEP derived criteria expressed as an annual geometric mean by
using the 75 th percentile of the annual geometric means from the long

- term dataset. FDEP demonstrated that setting the criteria at this percentile results in a magnitude that, when expressed as
an annual geometric mean not to be exceeded more than twice during a five -y ear period, will achieve
the targeted 10 percent error rate

. The magnitude component of the criteria (not to be exceeded more
than twice in a 5 year period) was established to allow for two separate assessments:

**•** As an annual geometric mean for a network o f stations in a specified segment of the waterbody;

and

**•** As an annual geometric mean for a single station in a given segment.

The two expressions of magnitude differ in the handling of within

- segment spatial variability. The
network average magnitude wa s calculated based on the distribution of spatially averaged annualNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 44 August 2010 geometric means, while the single site magnitude was calculated using the distribution of station annual
geometric means. Use of the network average magnitude is appropriate for assessing overall segment

status. However, a network average is only representative of overall segment status if it is relatively
homogeneous. If the segment is not well mixed, is very large, or contains localized nutrient sources,
then there is a potential that isolated areas of enrichment may be masked in the network spatial

average. Therefore, the single station annual geometric mean has the benefit of assessing localized
enrichment patterns. DEP has not decided whether both expressions of magnitude are need ed in all
cases, and we are seeking additional feedback from local experts and stakeholders.

Summary of the Proposed Criteria

FDEP intends to propose three sets of potential criteria: a) a long

- term geometric mean concentration; b) an annual geometric mea n of values from a network of stations over a given area, not to be exceeded

more than twice in a five

- year period; and c) an annual geometric mean of values from a single location,

not to be exceeded more than twice in a five

- year period.

These calculatio ns for the TME have not yet been completed.



### References

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- trophic state relationships in Florida Lakes. Publ. No. 56, Water Resources Research Center, University of Florida, Gainesville, FL.

Borum, J. 1996. Shallow waters and land/sea boundaries. In Jorgensen, B. and Richardson, K., (eds.). Eutrophication in Coastal Marine Ecosystems . American Geophysical Union, Washington, D.C., pp. 179

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CDM/DHI. 2002. Generation of a Curvilinear -o rthogonal Grid and Tidal Model for the Northern Coastal Basin. Final Report to the St. Johns River Water Management District, Palatka, FL.

Ceric, A., Winkler, S., and E. Lowe. 2007. Status and Trends in Water Quality at the St. Johns River Water Management District: Trophic State Assessment. Poster. AWRA Annual Meeting, New Mexico,

2007.

Cloern, J. E. 1991. Tidal stirring and phytoplankton bloom dynamics in an estuary. Journal of Marine
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Dame, R., M. Alber, D. Allen, M. Mallin, C. Montague, A. Lewitus, A. Chalmers, R. Gardner, C. Gilman, B. Kjerfve, J. Pinckney, and N. Smith. 2000. Estuaries of the South Atlantic coast of North America: their geographical signatures. Estuaries 23: 793

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Dettmann, E. H, 2001. Effect of water reside nce time on annual export and denitrification of nitrogen in estuaries: A model analysis. Estuaries 24, 481

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Dix, N. 2010. Nutrients, Chlorophyll, and Oysters in the Guana

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- Matanzas NERR. Presentation. Northeast Florida Numeric Nutrient Estuary Workshop, 2010.

Dix, N. and E. Phlips. 2010. Nutrients, chlorophyll, and oysters in the Guana Tolomato Matanz as NERR. Powerpoint presentation at the FDEP workshop on numeric nutrient criteria in estuaries and coastal waters. March 3, 2010, Jacksonville, Florida. www.dep.state.fl.us/wa ter/wqssp/nutrients/estuarine.htmNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 45 August 2010 Driscoll, A., C. John, F. Morris, and P. Victor. 2002. 2

- D curvilinear tidal model for the Northern Coastal Basin, northeast Florida. AWRA Spring Specialty Conference — Coastal Water Resources, New Orleans, La., May 13

– 15 , 2002.

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Florida Department of Environmental Protection. 2005. Water Quality Status Report. Tallahassee, FL 216pp.

Florida Department of Environmental Protection. 2009. Guana

- Tolomato

- Matanzas National Estuarine Research Reserve Management Plan. Tallahassee, FL 328 pp.

Fox, S.; Ceric, A.; Bourne, S.; Sucsy, P.; Montague, C.; Kinser, P. 2007. Assessment of Water Quality in the

Guana

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- Matanzas Estuary: A first application of the ACES workbench. Estuarine Research Federation Conference, November 2007, Providence , Rhode Island.

Frazel, D. 2009. Site Profile of the Guana

- Tolomato

- Matanzas National Estuarine Research Reserve. St Augustine, FL. 163pp.

Harwood, V. J. and D. V. Lim. 2005. Real

- Time Detection of Human Pathogens in the Guana

- Tolomato

Matanzas National E stuarine Research Reserve. A Final Report Submitted to the NOAA/UNH Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET), Durham, NH. 53pp.

Haydt, P. J. and Frazel, Inc. 2003. Northern Coastal Basin Surface Water Improvement and Management
Plan . St. Johns River Water Management District, Palatka, FL. 99pp.

Hitchcock, G. and D. Frazel. Unpublished raw data. Studies of Karenia brevis in northeast Florida. Chlorophyll, primary productivity, nutrient, and oxygen samples collected. O ctober 16

- 17, 2007.

John, C., and F. Morris. 1999. 2

- D finite element hydrodynamic and transport model application to
Tolomato John, C., and F. Morris. 1999. 2

- D finite element hydrodynamic and transport model application to Tolomato and Guana rivers. Seco nd Northern Coastal Basin Estuary workshop.
October 7– 8, 1999.

Johnson, P. G., J. Muller, and B. Holub. 1998. In Holub, B. (ed.), Northern Coastal Basins Reconnaissance Report . Report to St. Johns River Water Management District, Palatka, Florida.

Lee, G. F. and Jones, R., 1981. Application of the OECD eutrophication modeling approach to estuaries.

In Nielson, G. J. and Cronin, L. (eds.), Estuaries and Nutrients . Humana Press, pp. 549

- 568.

Mao, L. T. M., Freeman, R. and C. Basci. 2007. Hydrological Watershed Model for the Northern Coastal Basin North Project - Intracoastal Waterway (3I), Tolomato River (9D), and Matanzas River (9C) Planning Units. Technical Memorandum. Department of Water Resources, St. Johns River Water Management District Palatka, Florida. Ponte Vedra, FL. 151pp.

McFarlin, C. R., Brewer, S. 2008. Impact of Fertilzation on a Salt Marsh Food Web in Georgia. Estuaries

and Coasts: 31:313

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Miller, J.D. 2004. Status and trends of water quality in the Northern Coastal Basin. SJRWMD....Palatka,
Florida..Nutrient Criteria Technical Support Document Tolomato

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Draft 46 August 2010 NOAA (National Oceanic and Atmospheric Administration). 2007. Status Review of the Eastern Oyster ( Crassostrea virginica ), Report to the National Marine Fisheries Service, Northeast Regional Office, February 16, 2007. NOAA Tec h. Memo NMFS

- F/SPO

- 88, March 2007.

Ouyang, Y. 2005. Field estimation of groundwater discharge and nutrient load from selected areas in Duval and Clay Counties into the Lower St. Johns River. Draft SJRWMD report, SJRWMD, Division of Environmental Sciences, Water Resources Department, Palatka, Florida.

Phlips, E. J., N. Love, S. Badylak, P. Hansen, J. Lockwood, C. John, and R. Gleeson. 2004. A comparison of water quality and hydrodynamic characteristics fo the Guana

- Tolomato

- Matanzas national Estuarine Research Reserve and the Indian River Lagoon of Florida. Journal of Coastal Research

45: 93

- 109.

Pollman, C. D. and S. Roy. The contribution of atmospheric deposition to nutrient supply in the Lower St. Johns River, Florida, USA. Final report from Tetra Tech, Inc. to SJRWMD, Palatka, Florida.

Roman, C. T ., N. Jaworski, F. Short, S. Findlay, and R. Warren. 2000. Estuaries of the northeastern United States: habitat and land use signatures. Estuaries 23: 743

- 764.

Sheng, Y. P., B. Tutak, J. Davis, and V. Paramy gin. 2008. Circulation and flushing in the lagoonal system of
the Guana

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- Matanzas National Estuarine Research Reserve ( TME NERR), Florida. Journal of Coastal Research . 55: 9

- 25.

SJRWMD (St. Johns R. Water Management District). 2006. Resources map fi le of Northern Coastal Basins, Coastal Basins Section, Div. of Environmental Sciences, Palatka, Florida.

SJRWMD 2004a ?????

SJRWMD. 200 6

. Watershed Facts: Integrating environmental science and computer mapping technology

. Accessed at the SJRWMD Web site at http://www.sjrwmd.com/archydro/factsindex.html

. Based
on data retrieved on A ugust 1 7, 200 6

. Smith, V. H. 1998. Cultural eutrophication of inland, estuarine, and coastalwaters, p. 7 – 49. In M. L. Pace and P. M. Groffman (eds.), Successes, limitations, an d frontiers in ecosystem science. Springer

- Verlag.

St. Johns River Water Management District, Palatka, FL 124 pp. ?????

Steward, J. S. and Lowe, E. F., 2010. General empirical models for estimating nutrient load limits for
Florida’s estuaries and inland wat ers. Limnol. Oceanogr . 55, 433

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Steward, J. S., W. C. Green, and J. D. Miller. 2010 a . Using multiple lines of evidence for developing

numeric nutrient criteria for Tolomato

- Matanzas Estuary, Florida. Draft SJRWMD report to
FDEP, Tallahassee, Florida.

Steward, J. S., W. C. Green, and J. D. Miller. 2010 b. Using multiple lines of evidence for developing numeric nutrient criteria for Halifax River Estuary, Florida. Draft SJRWMD report to FDEP, Tallahassee, Florida.

Vollenweider, R. A. 1976. Advances in defining critical loading levels for phosphorus in lake eutrophication. Mem. Inst. Ital. Idrobiol . 33, 53

- 83.Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 47 August 2010 Appendix A. List of Species

- = Empty cell/no data Common Name Scientific Name Kingdom Plantae - Phylum Pterophyta (ferns)

- Carolina Mosquito Fern Azolla caroliniana

Cinnamon Fern Osmunda cinnamomea Royal Fern Osmunda regalis Golden Polypody Phlebodium aureum

Resurrection Fern Pleopeltis polypodioides var. michauxiana Lacy Bracken Pteridium aquilinum var. caudatum
Water fern Salvinia auriculata
Southern Shield Fern Thelypteris kunthii Shoestring Fern Vittaria lineata
Netted Chain Fern Woodwardia areolata Virginia Chain Fern Woodwardia virginica Phylum Pinophyta (cone

- bearing plants) - Red Cedar Juniperus virginiana
Sand Pine Pinus clausa
Slash Pine Pinus elliottii Longleaf pine Pinus palustris

Pond Pine Pinus serotina Loblolly Pine Pinus taeda Pond cypress Taxodium ascendens Phylum Magnoliophyta (flowering plants) - Blue Maidencane Amphicarpum muhlenbergianum
Florida Bluestem Andropogon floridanus Purple Bluestem Andropogon glomeratus var. glaucopsis Bushy Bluestem Andropogon glomeratus var. pumilus Broomsedge Andropogon longiberbis Splitbeard Bluestem Andropogon ternarius Broomsedge Bluestem Andropogon virginicus var. virginicus Green Dragon Arisaema dracontium Wiregrass Aristida beyrichiana Woollysheath Three

- awn Aristida lanosa
Bottlebrush Three

- awn Aristida spiciformis
Switchcane Arundinaria gigantea
Switch cane Arundinaria tecta

Common Oat Avena fatua

Bamboo Bambusa spp. Capillary Hairsedge Bulbostylis ciliatifolia Sandyfield Hairsedge Bulbostylis stenophylla Greenwhite sedge Carex albolutescens Sandywoods Sedge Carex dasycarpa Hammock Sedge Carex fissa var. aristata Long's Sedge Carex longii Blackedge Sedge Carex nigromarginataNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 48 August 2010 Common Name Scientific Name Southern Sandspur Cenchrus echinatus Coastal Sandspur Cenchrus incertus Sanddune Sandspur Cenchrus tribuloides Class Magnoliopsida (woody flowering plants) - Slender Woodoats Chasmanthium laxum var. laxum Spanglegrass Chasmanthium laxum var. sessiliflorum Sawgrass Cladium jamaicense Whitemouth Dayflower Commelina erecta Spring Coralroot Corallorhiza wisteriana Bermudagrass Cynodon dactylon

- Cyperus brevifolius Baldwin's Flatsedge Cyperus croceus Swamp Flatsedge Cyperus distinctus Yellow Nutgrass Cyperus esculentus Umbrella sedge Cyperus filicinus Globe sedge Cyperus globulosus Haspan Flatsedge Cyperus haspan Fragrant Flatsedge Cyperus odoratus Manyspike Flatsedge Cyperus polystachyos Pinebarren Flatsedge Cyperus retrorsus Nutgrass Cyperus rotundus Strawcolored Flatsedge Cyperus strigosus Tropical Flatsedge Cyperus surinamensis Fourangle Flatsedge Cyperus tetragonus Crowfootgrass Dactyloctenium aegyptium

Panicum Dicanthelium erectifolium Variable Witchgrass Dichanthelium commutatum Forked Witchgrass Dichanthelium dichotomum Hemlock Witchgrass Dichanthelium portoricense

- Dichanthelium sabulorum Southern Crabgrass Digitaria ciliaris Slender Crabgrass Digitaria filiformis var. filiformis Shaggy crabgrass Digitaria villosa Saltgrass Distichlis spicata Coast Cockspur Echinochloa walteri Baldwin's Spikerush Eleocharis baldwinii Yellow Spikerush Eleocharis flavescens Sand Spikerush Eleocharis montevidensis Viviparous Spikerush Eleocharis vivipara Indian Goosegrass Eleusine indica

Green

- fly Orchid Epidendrum magnoliae var. magnoliae Purple Lovegrass Eragrostis spectabilis Coastal Lovegrass Eragrostis virginica Centipedegrass Eremochloa ophiuroides Pinewoods Fingergrass Eustachys petraea Slender Fimbry Fimbristylis autumnalis Carolina Fimbry Fimbristylis carolinianaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 49 August 2010 Common Name Scientific Name Chesnut sedge Fimbristylis castanea Marsh Fimbry Fimbristylis spadicea Fringe rush Fimbristylis vahlii Dwarf Umbrellasedge Fuirena pumila Southern Umbrellasedge Fuirena scirpoidea Waterspider Orchid Habenaria repens Watergrass Hydrochloa carliniensis Fringed Yellow Stargrass Hypoxis juncea Blue flag Iris virginica Tapertip Rush Juncus acuminatus Leathery Rush Juncus coriaceus
Forked Rush Juncus dichotomus
Soft Rush Juncus effusus Bog Rush Juncus elliottii
Shore Rush Juncus marginatus Bighead Rush Juncus megacephalus Manyhead Rush Juncus polycephalus Creeping Rush Juncus repens
Black Needle Rush Juncus roemerianus Shortleaf Spikesedge Kyllinga brevifolia
Carolina Redroot Lachnanthes caroliniana Bogbuttons Lachnocaulon spp. Southern Cutgrass Leersia hexandra
Duckweed Lemna valdiviana Bearded Spangletop Leptochloa fascicularis Frog's

- bit; American Spongeplant Limnobium spongia Italian Ryegrass Lolium perenne Southern Watergrass Luziola fluitans Big moss Mayaca fluviatilis Gulf Hairawn Muhly Muhlenbergia capillaris var. filipes

Marine naiad Najas marina
Southern naiad Najas quadalupenis Woodsgrass Oplismenus hirtellus Bitter Panicgrass Panicum amarum
Beaked Panicum Panicum anceps Fall Panicgrass Panicum dichotomiflorum

Maidencane Panicum hemitomon Guineagrass Panicum maximum Torpedograss Panicum repens Redtop Panicum Panicum rigidulum Bluejoint Panicum Panicum tenerum Warty Panicgrass Panicum verrucosum Switchgrass Panicum virgatum Bull paspalum Paspalum boscianum Bahiagrass Paspalum notatum var. saurae Thin Paspalum Paspalum setaceum Vaseygrass Paspalum urvilleiNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 50 August 2010 Common Name Scientific Name

Pearl millet Pennisetum americanum Blackseed Needlegrass Piptochaetium avenaceum Annual Bluegrass Poa annua Rabbitsfootgrass Polypogon monspeliensis
Pickerelweed Pontederia cordata Sago pondweed Potamogeton pectinatus
Giant Orchid Pteroglossapsis ecristata Rose Natalgrass Rhynchelytrum repens Anglestem Beaksedge Rhynchospora caduca Starrush Whitetop Rhynchospora colorata Shortbristle Horned Beaksedge Rhynchospora corniculata Fascicled Beaksedge Rhynchospora fascicularis Threadleaf Beaksedge Rhynchospora filifolia Sandyfield Beaksedge Rhynchospora megalocarpa Southern Beaksedge Rhynchospora microcarpa Bunched Beaksedge Rhynchospora microcephala
Bald rush Rhynchospora nitens Widgeon grass Ruppia maritima Cabbage Palm; Sabal Palm Sabal palmetto Sugarcane Plumegrass Saccharum giganteum American Cupscale Sacciolepis striata Floating leaf sagittaria Sagittaria filiformis Grassy Arrowhead Sagittaria graminea var. graminea
Little Bluestem Schizachyrium scoparium var. scoparium
Saltmarsh bulrush Schoenoplectus robustus
Soft stem bulrush Schoenoplectus tabernaemontani Woolgrass Scirpus cyperinus
Netted Nutrush Scleria reticularis Tall Nutgrass Scleria triglomerata
Saw Palmetto Serenoa repens Giant Foxtail; Giant Bristlegrass Setaria magna
Knotroot Foxtail Setaria parviflora

Pointed blue eyed grass Sisyrinchium angustifolium

Annual Blue

- eyed Grass Sisyrinchium rosulatum
Earleaf Greenbrier Smilax auriculata
Saw Greenbrier Smilax bona

- nox Cat Greenbrier; Wild Sarsaparilla Smilax glauca Sarsaparilla Vine Smilax pumila Hog Brier; Bristly Greenbrier Smilax tamnoides Lopsided Indiangrass Sorghastrum secundum Smooth cordgrass Spartina alterniflora Smooth Cordgrass; Saltmarsh Cordgrass Spartina alterniflora var. glabra Sand Cordgrass Spartina bakeri Marshhay Cordgrass; Saltmeadow Cordgrass Spartina patens Prairie Wedgescale Sphenopholis obtusata

Ladies tresses Spiranthes praecox
Woodland Ladies'

- tresses Spiranthes sylvaticaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 51 August 2010 Common Name Scientific Name Spring Ladies'

- tresses Spiranthes vernalis Duckmeat; Dotted Duckweed Spirodela punctata Smutgrass Sporobolus indicus var. indicus Seashore Dropseed Sporobolus virginicus St. Augustine grass Stenotaphrum secundatum Yellow Hatpins Syngonanthus flavidulus Bartram's Airplant Tillandsia bartramii
Ballmoss Tillandsia recurvata
Southern Needleleaf Tillandsia setacea Spanish Moss Tillandsia usneoides Bluejacket; Ohio Spiderwort Tradescantia ohiensis Purpletop; Tall Redtop Tridens flavus var. flavus Arrowgrass Triglochin striata Purple Sandgrass Triplasis purpurea
Narrow leaved cattail Typha angustifolia Tropical cattail Typha domingensis
Broadleaf Cattail Typha latifolia

Sea Oats Uniola paniculata
Sixweeks Fescue Vulpia octoflora Bog mat Wolffiella gladiata Shortleaf Yelloweyed Grass Xyris brevifolia Richard's Yelloweyed Grass Xyris jupicai Spanish Bayonet Yucca aloifolia
Lawn Orchid Zeuxine strateumatica Slender Threeseed Mercury Acalypha gracilens Red Maple Acer rubrum Shyleaf; Joint

- vetch Aeschynomene americana

- Agalinis fasciculata
Hammock Snakeroot Ageratina jucunda Alligatorweed Alternanthera philoxeroides Pigweed Amaranthus spp. Common Ragweed Ambrosia artemisiifolia Fly poison Amianthium muscaetoxicum Toothcup Ammannia latifolia False indigo Amorpha fruticosa Peppervine Ampelopsis arborea Devil's Walkingstick Aralia spinosa Mexican Pricklypoppy Argemone mexicana
Savannah Milkweed Asclepias pedicellata
Velvetleaf Milkweed Asclepias tomentosa Smallflower Pawpaw Asimina parviflora Climbing Aster Aster carolinianus

Rice Button Aster Aster dumosus Swamp Aster; Elliott's Aster Aster elliottii
Annual Saltmarsh Aster Aster subulatus
Perennial Saltmarsh Aster Aster tenuifolius Whitetop Aster; Dixie Aster Aster tortifoliusNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 52 August 2010 Common Name Scientific Name
Walter's Aster Aster walteri Sea beach atriplex Atriplex arenaria Crested Saltbush; Seabeach Orach Atriplex pentandra Black Mangrove Avicennia germinans
Saltwater Falsewillow Baccharis angustifolia Silverling Baccharis glomeruliflora Salt Bush; Groundsel Tree; Sea Myrtle Baccharis halimifolia Blue Waterhyssop Bacopa caroliniana Smooth Waterhyssop; Herb

- of Grace Bacopa monnieri Saltwort; Turtleweed Batis maritima
Tarflower Bejaria racemosa

Rattan Vine Berchemia scandens Beggarticks Bidens alba Spanish Needles Bidens bipinnata Burrmarigold Bidens laevis

Crossvine Bignonia capreolata False Nettle; Bog Hemp Boehmeria cylindrica Red Spiderling; Wineflower Boerhavia diffusa Bushy Seaside Oxeye Borrichia frutescens

American Bluehearts Buchnera americana Tough bumelia Bumelia tenax
American Searocket Cakile edentula subsp. harperi American Beautyberry; French Mulberry Callicarpa americana

Matted Waterstarwort Callitriche peploides Trumpetcreeper Campsis radicans

Bitter cress Cardamine hirsuta Pennsylvania Bittercress Cardamine pensylvanica
Thistle Carduus spp.
Vanillaleaf Carphephorus odoratissimus Pignut Hickory Carya glabra Wild sensitive plant Cassia nictitans Sicklepod Cassia obtusifolia Sugarberry; Hackberry Celtis laevigata Spadeleaf; Coinwort Centella asiatica Spurred Butterfly Pea Centrosema virginianum
Buttonbush Cephalanthus occidentalis Florida Rosemary; Sand Heath Ceratiola ericoides
Coontail Ceratophyllum spp. Partridge Pea Chamaecrista fasciculata

Sensitive Pea Chamaecrista nictitans var. aspera

Dixie Sandmat Chamaesyce bombensis Eye

- bane; Hyssopleaf Sandmat Chamaesyce hyssopifolia Spotted Sandmat Chamaesyce maculata
Lamb's

- quarters Chenopodium album Mexican Tea;Pigweed Chenopodium ambrosioides Bull Thistle; Yellow Thistle Cirsium horridulum
Nuttall's Thistle Cirsium nuttalliiNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 53 August 2010 Common Name Scientific Name Sour Orange Citrus aurantium Butterfly Pea; Atlantic Pigeonwings Clitoria mariana Stinging Nettle; Tread

- softly; Finger

- rot Cnidoscolus stimulosus
Dwarf Canadian Horseweed Conyza canadensis var. pusilla
Golden Tickseed Coreopsis tinctoria Swamp dogwood Cornus stricta
Pursh's Rattlebox Crotalaria purshii
Rabbitbells Crotalaria rotundifolia Showy Rattlebox Crotalaria spectabilis Hogwort; Woolly Croton Croton capitatus Vente Conmigo Croton glandulosus Beach Tea; Gulf Croton Croton punctatus
Colombian Waxweed Cuphea carthagenensis Compact Dodder Cuscuta compacta Bigseed Alfalfa Dodder Cuscuta indecora
Gulfcoast Swallowwort Cynanchum angustifolium Western Tansymustard Descurainia pinnata Hoary Ticktrefoil; Beggarweed Desmodium incanum
Panicledleaf Ticktrefoil Desmodium paniculatum
Dixie Ticktrefoil Desmodium tortuosum
Threeflower Ticktrefoil Desmodium triflorum Carolina Ponysfoot Dichondra caroliniensis

Poor Joe Diodia teres Virginia Buttonweed Diodia virginiana

Common Persimmon Diospyros virginiana Wedgeleaf Whitlowgrass Draba cuneifolia
Dwarf Sundew Drosera brevifolia
Pink Sundew Drosera capillaris Oblong Twinflower Dyschriste oblongifolia False Daisy Eclipta prostrata Tall Elephantsfoot Elephantopus elatus

Fireweed Erechtites hieracifolia
Oakleaf Fleabane Erigeron quercifolius Baldwin's Eryngo Eryngium baldwinii Coralbean; Cherokee Bean Erythrina herbacea
Coastal white snakeroot Eupatorium aromaticum Dog Fennel Eupatorium capillifolium
Yankeeweed Eupatorium compositifolium
Falsefennel Eupatorium leptophyllum Mohr's Thoroughwort Eupatorium mohrii Roundleaf Thoroughwort Eupatorium rotundifolium Lateflorwering Thoroughwort Eupatorium serotinum
Slender Goldenrod Euthamia caroliniana Flat topped goldenrod Euthamia minor Florida Swampprivet Forestiera segregata Firewheel; Blanketflower Gaillardia pulchella Elliott's Milkpea Galactia elliottiiNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 54 August 2010 Common Name Scientific Name Eastern Milkpea Galactia regularis Downy Milkpea Galactia volubilis
Coastal Bedstraw Galium hispidulum
Stiff Marsh Bedstraw Galium tinctorium
Southern Beeblossom Gaura angustifolia Dwarf Huckleberry Gaylussacia dumosa Blue Huckleberry Gaylussacia frondosa var. tomentosa Dangleberry Gaylussacia nana
Yellow Jessamine Gelsemium sempervirens
Carolina Cranesbill Geranium carolinianum Narrowleaf Purple Everlasting Gnaphalium falcatum Rabbit Tobacco; Sweeteverlasting Gnaphalium obtusifolium Pennsylvania Everlasting Gnaphalium pensylvanicum Loblolly bay Gordonia lasianthus Rough Hedgehyssop Gratiola hispida Hedge hyssop Gratiola ramoa Innocence; Fairy Footprints Hedyotis procumbens Pigmypipes Monotropsis reynoldsiae Red Mulberry Morus rubra Wax Myrtle; Southern Bayberry Myrica cerifera Cutleaf Watermilfoil; Green Parrot's

- feather Myriophyllum pinnatum American White Waterlily Nymphaea odorata Swamp Tupelo Nyssa sylvatica var. biflora Seabeach Evening Primrose Oenothera humifusa Cutleaf Evening Primrose Oenothera laciniata Devil Joint; Cockspur Pricklypear Opuntia pusilla Erect Pricklypear Opuntia stricta Wild Olive; Devilwood Osmanthus americanus
Yellow Woodsorrel Oxalis corniculata
Violet wood sorrel Oxalis corymbosa
Wood sorrell Oxalis florida
Yellow wood sorrel Oxalis stricta Florida Pellitory Parietaria floridana
Baldwin's Nailwort Paronychia baldwinii Virginia Creeper; Woodbine Parthenocissus quinquefolia Purple Passionflower Passiflora incarnata Corkystem Passionflower Passiflora suberosa Red Bay Persea borbonia var. borbonia Swamp Bay Persea palustris
Oak Mistletoe Phoradendron leucarpum
Mistletoe Phoradendron serotinum Capeweed; Turkeytangle Fogfruit Phyla nodiflora
Drummond's Leafflower Phyllanthus abnormis
Chamber Bitter Phyllanthus urinaria Walter's Groundcherry Physalis walteri
American Pokeweed Phytolacca americana
Pokeweed Phytolacca rigidaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 55 August 2010 Common Name Scientific Name

Violet butterwort Pinguicula vulgaris
Grassleaf Goldenaster Pityopsis graminifolia English Plantain Plantago lanceolata Virginia Plantain Plantago virginica Camphorweed Pluchea camphorata Stinking Camphorweed Pluchea foetida
Saltmarsh fleabane Pluchea purpuracens Rosy Camphorweed Pluchea rosea

Painted

- leaf; Fire

- on

- the

- Mountain Poinsettia cyathophora Polygala Polygala cymosa
Procession Flower Polygala incarnata
Yellow milkwort Polygala lutea Orange milkwort Polygala nana
Racemed Milkwort Polygala polygama Hairy Smartweed Polygonum hirsutum Mild Waterpepper Polygonum hydropiperoides

Dotted Smartweed Polygonum punctatum Hairy Leafcup Polymnia uvedalia Rustweed; Juniperleaf Polypremum procumbens Little Hogweed Portulaca oleracea Pink purslane Portulaca pilosa Pink Purslane; Kiss

- Me

- Quick Portulaca pilosa
Combleaf Mermaidweed Proserpinaca pectinata Carolina Laurelcherry Prunus caroliniana Black Cherry Prunus serotina var. serotina
Flatwoods Plum Prunus umbellata Common Hoptree; Wafer Ash Ptelea trifoliata
Blackroot Pterocaulon pycnostachyum Mock Bishopsweed Ptilimnium capillaceum Carolina Desertchicory; False Dandelion Pyrrhopappus carolinianus Chapman's Oak Quercus chapmanii
Sand Live Oak Quercus geminata Laurel Oak; Diamond Oak Quercus laurifolia Myrtle Oak Quercus myrtifolia
Water Oak Quercus nigra
Live Oak Quercus virginiana Low Spearwort Ranunculus pusillus
Wild Radish Raphanus raphanistrum Maryland Meadowbeauty Rhexia mariana Winged Sumac Rhus copallinum Climbing Dollar

- weed; Least Snoutbean Rhynchosia minima Tropical Mexican Clover Richardia brasiliensis Rough Mexican Clover Richardia scabra Sawtooth Blackberry Rubus argutus Sand Blackberry Rubus cuneifolius Southern Dewberry Rubus trivialis
Carolina Wild Petunia Ruellia caroliniensisNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 56 August 2010 Common Name Scientific Name
Hastateleaf Dock Rumex hastatulus Swamp Dock Rumex verticillatus Marsh pink Sabatia bartramii Shortleaf Rosegentian Sabatia brevifolia
Smallflower Mock Buckthorn Sageretia minutiflora

Annual Glasswort Salicornia bigelovii Perennial Glasswort; Virginia Glasswort Salicornia perennis Perennial glasswort Salicornia virginica
Carolina Willow Salix caroliniana Prickly Russian Thistle Salsola kali subsp. pontica Tropical Sage Salvia coccinea Lyreleaf Sage Salvia lyrata American Elder; Elderberry Sambucus canadensis Pineland Pimpernel Samolus valerandi subsp. parviflorus
Blacksnakeroot Sanicula canadensis Southern soapberry Sapindus saponaria Soapberry Sapindus saponaria
Lizard's tail Saururus cernuus
Sweetbroom Scoparia dulcis Sicklepod; Coffeeweed Senna obtusifolia Bequilla Sesbania emerus Danglepod Sesbania herbacea Bladderpod; Bagpod Sesbania vesicaria Seapurslane Sesuvium portulacastrum Common Wireweed; Common Fanpetals Sida acuta Cuban Jute; Indian Hemp; Teaweed Sida rhombifolia Tough Buckthorn Sideroxylon tenax Sleepy Catchfly Silene antirrhina
Horsenettle Solanum carolinense var. carolinense Black Nightshade Solanum chenopodioides Tropical Soda Apple Solanum viarum
Canada Goldenrod Solidago canadensis var. scabra
Pinebarren Goldenrod Solidago fistulosa Chapman's Goldenrod Solidago odora var. chapmanii
Seaside Goldenrod Solidago sempervirens Spiny Sowthistle Sonchus asper
Common Sowthistle Sonchus oleraceus Shrubby False Buttonweed Spermacoce verticillata Roughfruit Scaleseed Spermolepis divaricata Florida Betony; Florida Hedgenettle Stachys floridana Trailing Fuzzybean Strophostyles helvula Sea Blite; Annual Seepweed Suaeda linearis Wood Sage; Canadian Germander Teucrium canadense Eastern Poison Ivy Toxicodendron radicans

Puncturevine Tribulus terrestris
Forked Bluecurls Trichostema dichotomum Hop clover Trifolium dubiumNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 57 August 2010 Common Name Scientific Name

White Clover Trifolium repens Clasping Venus' Lookingglass Triodanis perfoliata Humped Bladderwort Utricularia gibba Floating bladderwort Utricularia inflata Eastern Purple Bladderwort Utricularia purpurea Little Floating Bladderwort Utricularia radiata Zigzag Bladderwort Utricularia subulata Sparkleberry; Farkleberry Vaccinium arboreum Highbush Blueberry Vaccinium corymbosum Shiny Blueberry Vaccinium myrsinites Deerberry Vaccinium stamineum Woolly Mullein Verbascum thapsus
Wand Mullein Verbascum virgatum Purpletop Vervain Verbena bonariensis
Brazilian Vervain Verbena brasiliensis

Texas Vervain Verbena officinalis var. halei
Harsh Vervain Verbena scabra Frostweed; White Crownbeard Verbesina virginica

Giant Ironweed Vernonia gigantea
Fourleaf Vetch Vicia acutifolia Hairypod Cowpea Vigna luteola
White violet Viola affinis Bog White Violet Viola lanceolata Early Blue Violet Viola palmata
Common Blue Violet Viola sororia Summer Grape Vitis aestivalis

Muscadine Vitis rotundifolia
Southern Rockbell Wahlenbergia marginata
Hercules'

- club Zanthoxylum clava

- herculis Kingdom Animalia - Phylum Porifera (sea sponges) - Boring Sponge Cliona sp. Purple Sponge Haliclona permollis Sun Sponge Hymeniacidon heliophila Red Beard Sponge Microciona prolifera Phylum Cnidaria (jellyfishes and anemones) - Class Anthozoa (anemones and corals) - Brown Anemone Aiptasia pallida Anthopleura varioarmata GRM Astrangia danae GRM
Tricolor Anemone Calliactis tricolor
Sea Tube Anemone Ceriantheopsis americanus Sea Whip Leptogorgia virgulata Sea Pansy Renilla reniformis Class Hydrozoa (hydras) - Pink

- hearted hydroid Ectopleura crocea (Tubularia crocea) Portugese man

- of

- war Physalia physaliaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 58 August 2010 Common Name Scientific Name Class Scyphozoa (jellyfishes) - Moon Jelly Aurelia aurita
Sea Nettle Chrysaora quinquecirrha
Lions' Mane Medusa Cyanea capillata Cannonball jellyfish Stomolophus meleagris Phylum Ctenophora (comb jellies) - Beroe Beroe sp.
Sea Walnut Mnemiopsis leidyi Sea gooseberry Pleurobrachia pileus Phylum Platyhelminthes (flatworms) - Horseshoe Crab Worm Bdelloura candida Phylum Annelida (segmented worms)

Parchment Worm Chaetopterus variopedatus
Tube Worm Diopatra cuprea
Blood Worm Glycera americana Phylum Arthropoda (spiders, insects, crustaceans) -

Class Arachnida (spiders, scorpions, mites) - Grass spider Agelenopsis
Lone star tick Amblyomma americanum
Black -&- yellow argiope Argiope aurantia Black and yellow argiope Argiope aurantis Centruroides scorpion Centruroides spp. Florida Striped Bark Scorpion Centruroides Hentzi
Wood tick Dermacentor spp. Chigger/Redbug Eutrombicula spp. Crablike spiny orb weaver Gasteracantha spp. Brown Widow Spider Latrodectus geometricus Southern Black widow spider Latrodectus mactans Giant vinegarone Mastigoproctus giganteus Golden silk spider Nephila clavipes Green lynx spider Peucetia viridans Brown daddy long legs Phalangium opilio Daring jumping spider Phidippus audax
Sub

- phylum Crustacea (shrimp, crabs, lobsters) - Aviu Shrimp Acetes americanus carolinae Snapping Shrimp Alpheus heterochaelis Speckled Swimming Crab Arenaeus cribrarius Pillbug Armadillium spp. Square backed marsh crab Armases cinereum
Acorn Barnacle Semibalanus balanoides
Common Barnacle Balanus balanoides Ivory Barnacle Balanus eburneus
Barnacles Balanus spp.
Flame Box Crab Calappa flammea Carolina Ghost Shrimp Callichirus major
Red Blue Crab Callinectes bocourtiNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 59 August 2010 Common Name Scientific Name

Ornate Blue Crab Callinectes ornatus
Blue crab Callinectes sapidus
Lesser blue crab Callinectes similis

Indo

- Pacific Swimming Crab Charybdis hellerii Gray Barnacle Chthamalus fragilis Striped Hermit Crab Clibanarius vittatus Say mud crab Dyspanopeus sayi
Atlantic sand crab Emerita talpoida
Flat Mud Crab Eurypanopeus depressus
Broadback Mud Crab Eurytium limosum Brown shrimp Farfantepenaeus aztecus Pink shrimp Farfantepenaeus duorarum

Scuds Gammarus spp.
Calico Crab Hepatus epheliticus
Smooth Mud Crab Hexapanopeus augustifrons Veined Shrimp Hippolysmata wurdemanni
Duck Barnacle Lepas anatifera Portly Spider Crab Libinia emarginata
Wharf Roach Ligia exotica White shrimp Penaeus setiferus

Lucifer faxoni - Cinnamon river shrimp Macrobrachium acanthurus
Stone Crab Menippe spp.
Florida Stone crab Menippe mercenaria
Gulf Stone Crab Menippe adina

Neopanope texana sayi

- Ghost crab Ocypode quadrata
Mottled Shore Crab Pachygrapsus transversus
Banded Hermit Crab Pagurus annulipes Long clawed hermit crab Pagurus longicarpus
Flat

- Clawed Hermit Crab Pagurus pollicaris
Hermit crab Pagurus spp. Daggerblade Grass shrimp Palaemonetes pugio Panaeid shrimp Panaeus spp.
Common Mud Crab Panopeus herbstii
Furrowed Mud Crab Panopeus occidentalis
Green Porchelain Crab Petrolisthes armatus Sea Spider Phoxichilidium femoratum

Tube Pea Crab Pinnixa chaetopterana
Pea crab Pinnixa retinens
Mussel Crab Pinnotheres maculatus Oyster Crab Pinnotheres ostreum Tidal Spray Crab Plagusia depressa
Eastern Tube Crab Polyonyx gibbesi Iridescent Swimming Crab Portunus gibbesii Sargassum Crab Portunus sayi Blotched Swimming Crab Portunus spinimanusNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 60 August 2010 Common Name Scientific Name Crayish Procambarus spp.
Estuarine Mud Crab Rhithropanopeus harrisii
Wharf Crab Sesarma cinereum Purple Marsh Crab Sesarma reticulatum
Beach fleas Talorchestia spp. Arrow Shrimp Tozeuma carolinense
Mussel Pea Crab Tumidotheres maculatus

Red

- Jointed Fiddler Uca minax
Sand fiddler crab Uca pugilator
Atlantic Marsh Fiddler Uca pugnax Coastal Mud Shrimp Upogebia affinis Oyster Pea Crab Zaops ostreum Class Insecta (insects) - Green stink bug Acrosternum hilare
Luna moth Actias luna Eastern salt marsh mosquito Aedes sollicitans Salt marsh mosquitos Aedes taeniorhynchus Virescent green metallic bee Agapostemon virescens Gulf fritillary Agraulis vanillae Pink spotted hawk moth Agrius cingulata Eastern eyed click beetle Alaus oculatus
White Peacock Anartia jatrophae Common green darner Anax junius
Comet darner Anax longipes Mosquito Anopheles Polyphemus moth Antheraea polyphemus Honeybee Apis mellifera Gray green clubtail Arigomphus pallidus
Great southern white Ascia monuste
Io moth Automeris io Pipevine swallowtail Battus philenor Love bug Bibio
Bumblebee Bombus pensylvanicus Four spotted pennant Brachymesia gravida Fiery searcher Calosoma scrutator Black carpenter ant Camponotus pennsylvanicus Halloween pennant Celithemis eponina Double ringed pennant Celithemis verna
Deerflies Chrysops Mosquito Coquillettidia
Goldsmith beetle Cotalpa lanigera
Sand flies Culicoides
Monarch Danaus plexippus Virginia creeper sphinx Darapsa myron
Cow killer Dasymutilla occidentalis Northern walking stick Diapheromera femorata Rosy maple moth Dryocampa rubicundaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 61 August 2010 Common Name Scientific Name E. pond hawk Erythemis simplicicollis Blue dragonlet Erythrodiplax connata Little sulphur Eurema lisa Chigger Futrombicula spp.
Field cricket Gryllus pennsylvanicus Small whirligig beetle Gyrinus spp.
Ceraunus blue Hemiargus ceraunus Riparian earwig Labidura riparia
Silverfish Lepisma saccharina Giant waterbug Lethocerus americanus Marl pennant Macrodiplax balteata Tent caterpillar moth Malacosoma americanum Freshwater mosquitos Mansonia spp.
American carrion beetle Necrophila americana
Northern mole cricket Neocurtilla hexadactyla
Roseate skimmer Orthemis ferruginea
Blue dasher Pachydiplax longipennis Wandering glider Pantala flavescens

Giant swallowtail Papilio cresphontes E. Tiger swallowtail Papilio glaucus
Palamedes swallowtail Papilio palamedes
American cockroach Periplaneta americana
Scarab beetle Phanaeus vindex Cloudless sulphur Phoebis sennae
Phaon crescent Phyciodes phaon Paper wasp Polistes spp.
Giant root borer Prionus spp.
Flood

- water Mosquito Psorophora spp. Black ground beetle Pterostichus spp. Brown water scorpion Ranatra fusca

Termite Reticulitermes flavipes

Termites Reticultermes flavipes SE. Lubber grasshopper Romalea microptera Black/yellow mud dauber Sceliphron caementarium

Fire ant Solenopsis wagneri
Oleander moth Syntomeida epilais
Horseflies Tabanus spp. Violet masked glider Tramea carolina Common buckey Unonia coenia Long tailed skipper Urbanus proteus
Bella moth Utetheisa ornatrix bella American lady Vanessa virginiensis Yellowjacket Vespula spp. Carpenter bee Xylocopa virginica
Tersa moth Xylophanes tersa
Class Merostomata - Horseshoe crab Limulus polyphemusNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 62 August 2010 Common Name Scientific Name Mantis shrimp Squilla empusa
Class Gastropoda (snails) - Sea Hare Aplysia braziliana Striped Sea Slug Armina tigrina
False Cerith Batillaria minima
Variable Bittium Bittium varium
West Indian Bubble Bulla occidentalis Ragged Sea Hare Bursatella leachi
Knobbed Whelk Busycon carica Lightning Whelk Busycon sinistrum Sculptured Top

- Shell Calliostoma euglyptum
Tinted Cantharus Cantharus tinctus
Ladder Horn Shell Cerithdea scalariformis

Miniature Cerith Cerithiopsis greeni
Florida Cerith Cerithium floridanum Spiny Slipper Shell Crepidula aculeata Atlantic Slipper Shell Crepidula fornicata Eastern White Slipper Shell Crepidula plana
Giant Atlantic Cockle Dinocardium robustum Keyhold Limpet Diodora cayenensis Angulate Wentletrap Epitonium angulatum Banded Tulip Fasciolaria hunteria True Tulip Fasciolaria tulipa Mortons Egg Cockle Laevicardium mortoni Marsh periwinkle Littorina irrorata
Zebra Periwinkle Littorina ziczac
Common Marsh Snail Melampus bidentatus
Saltmarsh snail Melampus coffeus
Crown Conch Melongena corona
Atlantic Modulus Modulus modulus
Mud snail Nassarius obsoletus

Eastern Nassa Nassarius vibex
Olive Nerite Neritina reclivata Virgin Nerite Neritina virginea Impressed Odostome Odostomia impressa
Olive Shell Oliva sayana
Variable Dwarf Olive Olivella mutica
Horse Conch Pleuroploca gigantea Shark Eye Polinices duplicatus
Miniature Cerith Seila adamsi False Limpet Siphonaria pectinata Terrestrial gastrpod Succinea campestris
Florida Rock Shell Thais haemastoma floridana
Black

- Lined Trifora Triphora nigrocincta
Chestnut Turban Turbo castaneus Atlantic Oyster Drill Urosalpinx cinerea
Class Polyplacophora (chitons)

-Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 63 August 2010 Common Name Scientific Name

Eastern Chiton Chaetopleura apiculata Class Bivalvia (clams, mussels, oysters) - Common Atlantic Abra Abra aequelis Paper Mussel Amygdalum papyria Greedy Dove

- Shell Anachis avara

Fat Dove

- Shell Anachis obesa
Transverse Ark Anadara transversa Jingle Shell Anomia simplex Zebra Turkey Wing Arca zebra
Sawtooth Pen Clam Atrina serrata Angel Wing Barnea costata
Scorched Mussel Brachidontes exustus
Hooked Mussel Brachidontes recurvus

Cross

- Barred Venus Chione cancellata
Conrad's False Mussel Congeria leucophaeata
Contracted Corbula Corbula contracta Eastern Oyster Crassostrea virginica Angelwing Clam Cyrtopleura costata Coquina clam Donax variabilis
Disk Dosinia Dosinia discus
Jackknife Clam Ensis minor
Razor clams Ensis spp.
Comb Bittersweet Glycymeris pectinata
Marsh Mussel Guekensia demissa
Ribbed mussel Ischadium demissum Mahogany Date Mussel Lithophaga bisulcata
Calico clam Macrocallista maculata Wedge

- Shaped Martesia Martesia cuneiformis Southern Quahog Mercenaria campechiensis Quahog Mercenaria mercenaria

Lunar Dove

- Shell Mitrella lunata
Dwarf Surf Clam Mulinia lateralis
Pondersous Ark Noetia ponderosa Crested Oyster Ostrea equestris
Carolina Marsh Clam Polymesoda caroliniana
Florida Marsh Clam Pseudocyrena floridana Common Rangia Rangia cuneata Purplish Tagelus Tagelus divisus Stout Tagelus Tagelus plebius
Rose Petal Tellin Tellina lineata Class Cephalopoda (squids and octopus) - Squid Loligunculus brevis Atlantic Octopus Octopus vulgaris Phylum Bryozoa (Ectoprocta, moss animals) - Zoobotryon verticillatum - Rubber bryozoan Alcyonidium hauffi Anguinella palmata

-Nutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 64 August 2010 Common Name Scientific Name Bugula neritina - Common sea mat Membranipora tenuis Schizoporella errata - Phylum Echinodermata - Common Starfish Asterias forbesi Margined Sea Star Astropecten articulatus

Lines Sea Star Luidea clathrata Class Echnioidea (sea urchins) - Purple Spined Sea Urchin Arbacea punctulata
Variable Urchin Lytechinus variegatus
Sand Dollar Mellita quinquesperforata Class Holothuroidea (sea cucumbers)

- Common Thyone Thyone briatus Class Ophiuroidea (brittle stars)

- Angular brittle star Ophiothrix angulata Phylum Hemichordata (acorn worms) - Southern acorn worm Ptychodera bahamensis Phylum Chordata (vertebrates and relatives) - Class Ascidiacea (sea squirts)

- Didemnum candidum - Mangrove Tunicate Ecteinascidia turbinata Common sea grape Molgula manhattensis Pleated sea squirt Styela plicata Sea pork Amaroucium stellatum Class Cephalochordata (lancelets) - Caribean Lacelet Branchiostoma caribaeum
Sub

- phylum Vertebrate (vertebrates) - Class Agnatha (jawless fishes) - Sea Lamprey Petromyzon marinus Class Chondrichthyes (cartilaginous fishes) - Shortnose Sturgeon Acipenser brevirostrum Gulf Sturgeon Acipenser oxyrinchus desotoi Spinner shark Carcharhinus brevipinna
Bull shark Carcharhinus leucas Blacktip shark Carcharhinus limbatus
Sandbar shark Carcharhinus plumbeus Southern Sting Ray Dasyatis americana Atlantic stingray Dasyatis sabina Smooth butterfly ray Gymnura micrura
Lemon Shark Negaprion brevirostris
Sand Shark Odontaspis taurus
Clearnose Skate Raja eglanteria
Atlantic Guitarfish Rhinobatos lentiginosus Cownose ray Rhinoptera bonasus
Hammerhead sharks Shyrna spp. Scalloped Hammerhead Sphyrna lewini
Great Hammerhead Sphyrna mokarranNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 65 August 2010 Common Name Scientific Name

Bonnethead Sphyrna tiburo Super

- class Osteichthyes (bony fishes) - Seargeant major Abudefduf saxatilis
Cowfish Acanthostracion quadricornis
Lined sole Achirus lineatus
Bonefish Albula vulpes Hickory shad Alosa mediocris
Filefish Aluterus spp.
Yellow bullhead Ameiurus natalis
Bowfin Amia calva Striped anchovy Anchoa hepsetus Bay anchovy Anchoa mitchilli Anchovy Anchoa spp.
Oscillated flounder Ancylopsetta quadrocellata
American Eel Anguilla rostrata Sheepshead Archosargus probatocephalus
Sea catfish Ariopsis felis S. stargazer Astroscopus y

- graecum Gafftopsail catfish Bagre marinus Silver perch Bairdiella chrysoura Triggerfish Balistes spp. Frillfin goby Bathygobius soporator
Atlantic menhaden Brevoortia tyrannus Yellow jack Carangoides bartholomaei Crevalle jack Caranx hippos

Horse

- eye jack Caranx latus
Snook Centropomus undecimalis
Rock sea bass Centropristis philadelphica
Black sea bass Centropristis striata Atlantic spadefish Chaetodipterus faber Striped blenny Chasmodes bosquianus Florida blenny Chasmodes saburrae Atlantic bumper Chloroscombrus chrysurus Striped Burrfish Cilomycterus schoepfi Spotted whiff Citharichthys macrops Bay whiff Citharichthys spilopterus Common Dolphin Coryphaena hippurus Spotted sea trout Cynoscion nebulosus
Atlantic weakfish Cynoscion regalis Sheepshead minnow Cyprinodon variegatus Irish pompano Diapterus auratus Striped mojarra Diapterus plumieri
Balloon fish Diodon holocanthus Dwarf sand perch Diplectrum bivittatum Sand perch Diplectrum formosum Silver porgy Diplodus argenteus Spottail pinfish Diplodus holbrookiNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 66 August 2010 Common Name Scientific Name

Gizzard shad Dorosoma cepedianum
Threadfin shad Dorosoma petenense
Sharksucker Echeneis naucrates Ladyfish Elops saurus Nassau Grouper Ephinephelus striatus Goliath grouper Epinephelus itajara Groupers Epinephelus spp.
Lake chubsucker Erimyzon sucetta Fringed flounder Etropus crossotus Silver jenny Eucinostomus gula Tidewater mojarra Eucinostomus harengulus Slender mojarra Eucinostomus jonesi Spotfin mojarra Eugerres plumieri Flying halfbeak Euleptorhamphus velox Little tunny Euthynnus alleteratus Golden topminnow Fundulus chrysotus
Marsh killifish Fundulus confluentus
Gulf killifish Fundulus grandis Mummichog Fundulus heteroclitus Striped/longnose killifish Fundulus majalis Tiger shark Galeocerdo cuviera Mosquitofish Gambusia affinis Eastern mosquitofish Gambusia holbrooki Yellowfin Mojarra Gerre cinereus
Skilletfish Gobiesox strumosus Violet goby Gobioides broussonetii Darter goby Gobionellus boleosoma Highfin goby Gobionellus oceanicus Freshwater goby Gobionellus shufeldti Emerald goby Gobionellus smaragdus Marked goby Gobionellus stigmaticus Naked goby Gobiosoma bosc Twoscale goby Gobiosoma longipala Code goby Gobiosoma robustum

Grunts Haemulon spp.
Scaled sardine Harengula jaguana
Least killifish Heterandria formosa
Lined seahorse Hippocampus erectus Barred blenny Hypleurochilus bermudensis
American halfbeak Hyporhamphus meeki Feather blenny Hypsoblennius hentz
Brown bullhead Ictalurus nebulosus FL. Flagfish Jordanella floridae Hairy blenny Labrisomus nuchipinnis
Trunkfish Lactophrys trigonus
Pinfish Lagodon rhomboides
Warmouth Lapomis gulosusNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 67 August 2010 Common Name Scientific Name

Banded drum Larimus fasciatus Spot Leiostomus xanthurus Longnose gar Lepisosteus osseus
Redbreasted sunfish Lepomis auritus Bluegill Lepomis macrochirus
Redear sunfish Lepomis microlophus Stumpknocker Lepomis punctatus Tripletail Lobotes surinamensis Red Snapper Lutjanus campechanus Gray snapper Lutjanus griseus Lane snapper Lutjanus synagris
Atlantic Manta Manta birostris Tarpon Megalops atlanticus Rough silvereside Membras martinica
Tidewater Silverside Menidia beryllina
Atlantic silverside Menidia menidia
Silverside Menidia spp. Whiting Menticirrhus americanus Gulf kingfish Menticirrhus littoralis Northern kingfish Menticirrhus saxatilis Clown goby Microgobius gulosus Green goby Microgobius thalassinus Opposum pipefish Microphis brachyurus
Atlantic croaker Micropogonias undulatus Largemouth bass Micropterus floridanus
Filefish Monacanthus spp. Striped mullet Mugil cephalus
White mullet Mugil curema Gag Myctoperca microlepis Speckled worm eel Myrophis punctatus
Golden shiner Notemigonus crysoleucus
Polka

- dot batfish Ogcocephalus cubifrons Leatherjack Oligoplites saurus Shrimp eel Ophichthus gomesi
Cusk

- eel Ophidion spp. Atlantic thread herring Opisthonema oglinum Oyster toadfish Opsanus tau Pigfish Orthopristis chrysoptera
Gulf flounder Paralichthys albigutta
Summer flounder Paralichthys dentatus
S. flounder Paralichthys lethostigma
Broad flounder Paralichthys squamilentus
Harvest fish Peprilus paru Sailfin molly Poecilia latipinna
Mollies Poecilia spp.
Blackdrum Pogonias cromis
Bluefish Pomatomus saltatrixNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 68 August 2010 Common Name Scientific Name

Northern searobin Prionotus carolinus Striped searobin Prionotus evolans
Blackfin searobin Prionotus rubio Leopard searobin Prionotus scitulus Bighead searobin Prionotus tribulus Short bigeye Pristigenys alta
Cobia Rachycentron canadum

Remora Remora remora

Bonito Sarda sarda Spanish sardine Sardinella aurita

Red drum Sciaenops ocellatus King Mackeral Scomberomorus cavalla Spanish mackerel Scomberomorus maculatus
Barbfish Scorpaena brasiliensis Spotted scorpionfish Scorpaena plumieri Bigeye scad Selar crumenophthalmus
Atlantic moonfish Selene setapinnis
Lookdown Selene vomer Amberjack Seriola dumerili
Florida Puffer Sphoerodies nephelus Northernpuffer Sphoeroides maculatus Southern puffer Sphoeroides nephelus Checkered puffer Sphoeroides testudineus
Great barracuda Sphyraena barracuda Guaguanche Sphyraena guachancho

Star drum Stellifer lanceolatus
Planehead filefish Stephanolepis hispida
Atlantic needlefish Strongylura marina
Redfin needlefish Strongylura notata Blackcheek tonguefish Symphurus plagiusa Dusky pipefish Syngnathus floridae N. pipefish Syngnathus fuscus Chain pipefish Syngnathus louisianae Gulf pipefish Syngnathus scovelli
Inshore lizardfish Synodus foetens Pompano Trachinotus carolinus

Permit Trachinotus falcatus Rough scad Trachurus lathami
Atlantic cutlassfish Trichiurus lepturus Hogchoker Trinectes maculatus
Southern hake Urophycis floridana
Class Amphibia (frogs and salamanders)

- Florida Cricket frog Acris gryllus dorsalis
Mole salamander Ambystoma talpoideum
Oak toad Bufo quercicus
Southern Toad Bufo terrestris Greenhouse frog Eleutherodactylus planirostrisNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 69 August 2010 Common Name Scientific Name
Dwarf salamander Eurycea quadradigitata

Narrow

- mouthed toad Gastrophryne carolinensis Green tree frog Hyla cinerea Pine woods tree frog Hyla femoralis Barking treefrog Hyla gratiosa Squirrel tree frog Hyla squirella Striped newt Notophthalmus perstriatus Cuban tree frog Osteopilus septentrionalis Spring peeper Pseudacris crucifer Florida Chorus frog Pseudacris nigrita verrucosa Little grass frog Pseudacris ocularis Gopher Frog Rana capito Bullfrog Rana catesbeiana Pig frog Rana grylio Florida leopard frog Rana sphenocephala Eastern spadefoot Scaphiopus holbrookii Class Reptilia (reptiles) - Florida cottonmouth Agkistrodon piscivorus conanti American Alligator Alligator mississippiensis
Green anole Anolis carolinensis
Brown anole Anolis sagrei
Florida Softshell Turtle Apalone ferox

Six

- lined racerunner Aspidoscelis sexlineatus Loggerhead sea turtle Caretta caretta
Florida scarlet snake Cemophora coccinea
Green sea turtle Chelonia mydas Florida Snapping turtle Chelydra serpentina osceola
Florida cooter Chrysemys floridana Spotted Turtle Clemmys guttata
Black racer Coluber constrictor priapus
Eastern Diamondback rattlesnake Crotalus adamanteus
Chicken turtle Deirochelys reticularia
Leatherback sea turtle Dermochelys coriacea Southern Ringneck snake Diadophis punctatus punctatus Eastern Indigo snake Drymarchon corais couperi
Red rat snake Elaphe guttata
SE five

- lined skink Eumeces inexpectatus
Broad

- headed skink Eumeces laticeps
Eastern Mud snake Farancia abacura abacura Gopher tortoise Gopherus polyphemus
Florida red bellied turtle Grysemys nelsoni Mediteranian gecko Hemidactylus garnotii Striped mud turtle Kinosternon bauri
Florida Mud turtle Kinosternon subrubrum steindachneri
Eastern Mud turtle Kinosternon subrubrum subrubrum Eastern King snake Lampropeltis getula getula Scarlet king snake Lampropeltis triangulum elapsoidesNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 70 August 2010 Common Name Scientific Name Kemp's Ridley Lepidochelys kempii Diamondback terrapin Malaclemys terrapin centrata E. Coachwhip Masticophis flagellum flagellum
Coral Snake Micrurus fulvius
Fl. water snake Nerodia fasciata pictiventris Rough green snake Opheodrys aestivus
E. Glass lizard Ophisaurus ventralis
Corn snake Pantherophis guttata guttata
Yellow rat snake Pantherophis obsoleta quadrivittata FL. Redbelly turtle Pseudemys nelsoni
Peninsula cooter Pseudemys peninsularis
Pine woods snake Rhadinaea flavilata

Ground skink Scincella lateralis

Greater siren Siren lacertina Dusky pigmy rattlesnake Sistrurus miliarius barbouri
FL. Box turtle Terrapene carolina bauri
Peninsula ribbon snake Thamnophis sauritus nitae
E. Garter snake Thamnophis sirtalis
Yellow

- bellied slider Trachemys scripta
Florida softshell Apalone ferox Class Aves (birds) - Cooper's hawk Accipiter cooperii Sharp

- shinned Hawk Accipiter striatus Spotted Sandpiper Actitis macularia
Red

- winged Blackbird Agelaius phoeniceus
Wood Duck Aix sponsa Roseate Spoonbill Ajaia ajaja Saltmarsh Sharp

- tailed Sparrow Ammodramus caudacutus Seaside Sparrow Ammodramus maritimus Nelson's Sharp

- tailed Sparrow Ammodramus nelsoni Grasshopper Sparrow Ammodramus savannarum
Northern Pintail Anas acuta American Widgeon Anas americana
Northern Shoveler Anas clypeata

Green

- winged Teal Anas crecca
Blue

- winged Teal Anas discors
Mottled Duck Anas fulvigula
Mallard Anas platyrhynchos
American black duck Anas ruprides
American black duck Anas ruprides
Gadwall Anas strepera Anhinga Anhinga anhinga American Pipit Anthus rubescens Water pipit Anthus spinoletta Florida Scrub Jay Aphelocoma coerulescens Fox sparrow Apsserella iliaca Limpkin Aramus guaraunaNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 71 August 2010 Common Name Scientific Name Ruby

- throated Hummingbird Archilochus colubris Great Egret Ardea alba
Great Blue Heron Ardea herodias Ruddy Turnstone Arenaria interpres Lesser Scaup Aythya affinis

Redhead Aythya americana Ring

- necked Duck Aythya collaris Greater Scaup Aythya marila
Canvasback Aythya valisineria
Tufted Titmouse Baeolophus bicolor Cedar Waxwing Bombycilla cedrorum

American Bittern Botaurus lentiginosus

Brant Branta bernicla
Great Horned Owl Bubo virginianus Cattle Egret Bubulcus ibis
Bufflehead Bucephala albeola Common Goldeneye Bucephala clangula

Red

- tailed Hawk Buteo jamaicensis
Red

- shouldered Hawk Buteo lineatus

Green Heron Butorides virescens Sanderling Calidris alba
Dunlin Calidris alpina

Red Knot Calidris canutus Western Sandpiper Calidris mauri Least Sandpiper Calidris minutilla Semipalmated Sandpiper Calidris pusilla
Chuck

- will's

- widow Caprimulgus carolinensis Whip

- poor

- will Caprimulgus vociferus
Northern Cardinal Cardinalis cardinalis
American Goldfinch Carduelis tristis Purple finch Carpodacus purpureus Turkey Vulture Cathartes aura Veery Catharus fuscescens

Hermit Thrush Catharus guttatus Gray

- cheeked Thrush Catharus minimus
Swainson's Thrush Catharus ustulatus
Willet Catoptrophorus semipalmatus Belted Kingfisher Ceryle alcyon Chimney Swift Chaetura pelagica Piping Plover Charadrius melodus Semipalmated Plover Charadrius semipalmatus
Killdeer Charadrius vociferus
Wilson's Plover Charadrius wilsonia

Snow Goose Chen caerulescens
Black Tern Chlidonias niger Lark Sparrow Chondestes grammacus Common Nighthawk Chordeiles minorNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 72 August 2010 Common Name Scientific Name

Northern Harrier Circus cyaneus
Marsh Wren Cistothorus palustris Sedge Wren Cistothorus platensis
Yellow

- billed Cuckoo Coccyzus americanus
Northern Flicker Colaptes auratus
Northern Bobwhite Colinus virginianus Rock Pigeon Columbia livia

Common Ground

- Dove Columbina passerina

Eastern Wood

- Pewee Contopus virens
Black Vulture Coragyps altratus

American Crow Corvus brachyrhynchos
Fish Crow Corvus ossifragus Blue Jay Cyanocitta cristata
Tundra swan Cygnus columbianus
Black

- bellied Whistling Duck Dendrocygna autumnalis Fulvous Whistling

- Duck Dendrocygna bicolor
Black

- throated Blue Warbler Dendroica caerulescens Bay

- breasted Warbler Dendroica castanea
Cerulean Warbler Dendroica cerulea
Yellow

- rumped Warbler Dendroica coronata
Prairie Warbler Dendroica discolor
Yellow

- throated Warbler Dendroica dominica
Blackburnian Warbler Dendroica fusca
Kirtland's Warbler Dendroica kirtlandii Magnolia Warbler Dendroica magnolia
Palm Warbler Dendroica palmarum
Chestnut

- sided Warbler Dendroica pensylvanica
Yellow Warbler Dendroica petechia
Pine Warbler Dendroica pinus Blackpoll Warbler Dendroica striata Cape May Warbler Dendroica tigrina
Black

- throated Green Warbler Dendroica virens
Bobolink Dolichonyx oryzivorus Pileated Woodpecker Dryocopus pileatus Gray Catbird Dumetella carolinensis
Little Blue Heron Egretta caerulea Reddish Egret Egretta rufescens Snowy Egret Egretta thula
Tricolored Heron Egretta tricolor
Swallow

- tailed Kite Elanoides forficatus

White Ibis Eudocimus albus
Merlin Falco columbarius Peregrine Falcon Falco peregrinus
American Kestrel Falco sparverius Magnificent Frigate bird Fregata magnificens

American Coot Fulica americana Common Snipe Gallinago gallinagoNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 73 August 2010 Common Name Scientific Name

Common Moorhen Gallinula chloropus

Common Loon Gavia immer
Red

- throated Loon Gavia stellata
Common Yellowthroat Geothlypis trichas
Florida Sandhill Crane Grus canadensis pratensis
Blue Grosbeak Guiraca caerulea American Oystercatcher Haematopus palliatus Bald Eagle Haliaeetus leucocephalus

Worm

- eating Warbler Helmitheros vermivora
Black

- necked Stilt Himantopus mexicanus
Barn Swallow Hirundo rustica
Wood Thrush Hylocichla mustelina
Baltimore Oriole Icterus galbula
Orchard Oriole Icterus spurius

Least Bittern Ixobrychus exilis
Dark

- eyed Junco Junco hyemalis Loggerhead Shrike Lanius ludovicianus Herring Gull Larus argentatus Laughing Gull Larus atricilla Ring

- billed Gull Larus delawarensis Lesser black backed gull Larus fuscus
Great Black

- backed Gull Larus marinus Bonaparte's Gull Larus philadelphia
Short

- billed Dowitcher Limnodromus griseus Long

- billed Dowitcher Limnodromus scolopaceus
Marbled Godwit Limosa fedoa Hooded Merganser Lophodytes cucullatus Wild Turkey Melagris gallopavo

Red

- bellied Woodpecker Melanerpes carolinus

Red

- headed Woodpecker Melanerpes erythrocephalus
White

- winged Scoter Melanitta fusca
Black Scoter Melanitta nigra
Surf Scoter Melanitta perspicillata Swamp Sparrow Melospiza georgiana Song Sparrow Melospiza melodia

Red

- breasted Merganser Mergus serrator Northern Mockingbird Mimus polyglottos
Black

- and

- white Warbler Mniotilta varia

Brown

- headed Cowbird Molothrus ater
Northern Gannet Morus bassanus
Wood Stork Mycteria americana Great Crested Flycatcher Myiarchus crinitus
Whimbrel Numenius phaeopus
Yellow

- crowned Night

- Heron Nyctanassa violacea
Black

- crowned Night

- Heron Nycticorax nycticorax Yellow crowned night heron Nycticorax violaceous
Wilson's Storm

- Petrel Oceanites oceanicusNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 74 August 2010 Common Name Scientific Name

Eastern Screech

- Owl Otus asio Ruddy Duck Oxyura jamaicensis Osprey Pandion haliaetus
Northern Parula Parula americana House Sparrow Passer domesticus Savannah Sparrow Passerculus sandwichensis Painted Bunting Passerina ciris Indigo Bunting Passerina cyanea
American White Pelican Pelecanus erythrorhynchos
Brown Pelican Pelecanus occidentalis
Double

- crested Cormorant Phalacrocorax auritus

Rose

- breasted Grosbeak Pheucticus ludovicianus

Red

- Cockaded Woodpecker Picoides borealis Downy Woodpecker Picoides pubescens Hairy Woodpecker Picoides villosus
Eastern Towhee Pipilo erythrophthalmus Scarlet Tanager Piranga olivacea Summer Tanager Piranga rubra Snow Bunting Plectrophenax nivalis Glossy Ibis Plegadis falcinellus
Black

- bellied Plover Pluvialis squatarola

Horned grebe Podiceps auritus
Red

- necked Grebe Podiceps grisegena
Pied

- billed Grebe Podilymbus podiceps
Carolina Chickadee Poecile carolinensis
Blue

- gray Gnatcatcher Polioptila caerulea Vesper Sparrow Pooecetes gramineus Purple Gallinule Porphyrula martinica

Sora Porzana carolina Purple Martin Progne subis Prothonotary Warbler Protonotaria citrea

Boat

- tailed Grackle Quiscalus major
Common Grackle Quiscalus quiscula King rail Rallus elegans Virginia Rail Rallus limicola Clapper Rail Rallus longirostris

American Avocet Recurvirostra americana Ruby

- crowned Kinglet Regulus calendula
Golden

- crowned Kinglet Regulus satrapa
Black Skimmer Rynchops niger
Eastern Phoebe Sayornis phoebe
American Woodcock Scolopax minor
Ovenbird Seiurus aurocapillus
Louisiana Waterthrush Seiurus motacilla
Northern Waterthrush Seiurus noveboracensis

American Redstart Setophaga ruticilla
Eastern Bluebird Sialia sialisNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 75 August 2010 Common Name Scientific Name

Red breasted nuthatch Sitta canadensis

Brown

- headed Nuthatch Sitta pusilla
Yellow

- bellied Sapsucker Sphyrapicus varius Chipping Sparrow Spizella passerina Field sparrow Spizella pusilla Northern Rough

- winged Swallow Stelgidopteryx serripennis

Least Tern Sterna antillarum Caspian Tern Sterna caspia

Roseate Tern Sterna dougallii
Forster's Tern Sterna forsteri

Common Tern Sterna hirundo Royal Tern Sterna maxima
Gull

- billed Tern Sterna nilotica
Sandwich Tern Sterna sandvicensis
Eurasian Collared

- Dove Streptopelia decaocto
Barred owl Strix varia
Eastern Meadowlark Sturnella magna European Starling Sturnus vulgaris Northern rough winged swallow Telgidopteryx serripennis
Carolina Wren Thryothorus ludovicianus
Brown Thrasher Toxostoma rufum
Tree Swallow Trachycineta bicolor Lesser Yellowlegs Tringa flavipes Greater Yellowlegs Tringa melanoleuca Solitary Sandpiper Tringa solitaria

House Wren Troglodytes aedon
American Robin Turdus migratorius Gray Kingbird Tyrannus dominicensis Eastern Kingbird Tyrannus tyrannus Western Kingbird Tyrannus verticalis
Barn Owl Tyto alba Orange

- crowned Warbler Vermivora celata
Golden

- winged Warbler Vermivora chrysoptera
Tennessee Warbler Vermivora peregrina
Blue

- winged Warbler Vermivora pinus
Nashville Warbler Vermivora ruficapilla
Yellow

- throated Vireo Vireo flavifrons
White

- eyed Vireo Vireo griseus
Red

- eyed Vireo Vireo olivaceus
Blue

- headed Vireo Vireo solitarius
Hooded Warbler Wilsonia citrina
Wilson's Warbler Wilsonia pusilla Mourning Dove Zenaida macroura
White

- throated Sparrow Zonotrichia albicollis
White

- crowned Sparrow Zonotrichia leucophrys Class Mammalia (mammals) - Right whale Balaena glacialisNutrient Criteria Technical Support Document Tolomato

– Matanzas Estuary

Draft 76 August 2010 Common Name Scientific Name

Short tail shrew Blarina brevicauda Rafinesque's Big

- Eared Bat Corynorhinus rafinesquii

Nine

- banded armadillo Dasypus novemcinctus Virginia opossum Didelphis virginiana Atlantic right whale Eubalaena glacialis Southeastern pocket gopher Geomys pinetis S. Flying squirrel Glaucomys volans Pygmy Sperm Whale Kogia breviceps

E. Red Bat Lasiurus borealis

River otter Lontra canadensis
Bobcat Lynx rufus Humpback whale Megaptera novaeangliae Striped skunk Mephitis mephitis

House mouse Mus musculus
Florida mink Mustela vison lutensis
Florida Mink Mustela vison mink S.E. Myotis Myotis austroriparius
Round tailed muskrat Neofiber alleni

Eastern woodrat Neotoma floridana

Seminole bat Nycteris seminolis
Golden mouse Ochrotomys nuttalli
White

- tailed deer Odocoileus virginianus
Marsh rice rat Oryzomys palustris

Cotton mouse Peromyscus gossypinus gossypinus
Anastasia Island Beach Mouse Peromyscus polionotus phasma
Old field mouse Peromyscus polionotus polionotus
Florida mouse Podomys floridanus

Raccoon Procyon lotor Norway rat Rattus norvegicus
Black rat Rattus rattus
Eastern harvest mouse Reithrodontomys humulis
Eastern mole Scalopus aquaticus Gray squirrel Sciurus carolinensis Hispid cotton rat Sigmodon hispidus
Southeastern shrew Sorex longirostris Eastern spotted skunk Spilogale putorius Feral pig Sus scrofa
Eastern Cottontail Sylvilagus floridanus
Marsh rabbit Sylvilagus palustris

West Indian manatee Trichechus manatus
Bottle

- nosed dolphin Tursiops truncatus Gray fox Urocyon cinereoargenteus
Florida Black Bear Ursus americanus floridanus
Red fox Vulpes vulpes