l..
REPORT Study Title DETERMINATION
OF PHYSICO-CHEMICAL PROPERTIES
OF TRANS-1-CHLOR0-3,3,3- TRIFLUOROPROPENE • Validation
of an analytical method

• Explosive properties • Melting and boiling temperature

• Auto-Ignition temperature • Water solubility

• Oxidizing properties • Flash-point

• Hydrolysis as a function of pH • Flammability (contact with water)

• Adsorption coefficient • Pyrophorlc properties Author Dr
. K.A. Oudhoff Study completion date 07 July 2010 Test Facility NOTOXB

. V. Hambakenwetering

7 5231 DD 's-Hertogenbosch The Netherlands Laboratory Project Identification NOTOX Project 492820 NOT OX Substance 202044/
A - Page

1 of 37

-Trans-1-chloro-3,3,3-trifluoropropeneNOTOXProject4928201.CONTENTS1.CONTENTS...........................................................................................................................22.STATEMENTOFGLPCOMPLIANCE..................................................................................43.QUALITYASSURANCESTATEMENT.................................................................................54.SUMMARY............................................................................................................................65.INTRODUCTION...................................................................................................................85.1.Preface........................................................................................................................85.2.Aimofthestudy...........................................................................................................85.3.Storageandretentionofrecordsandmaterials..........................................................86.MATERIALS..........................................................................................................................96.1.Testsubstance............................................................................................................96.2.Electronicdatacapture................................................................................................96.3.Listofdeviations..........................................................................................................97.VALIDATIONOFANANALYTICALMETHOD....................................................................107.1.Guideline...................................................................................................................107.2.Reagents...................................................................................................................107.3.Performanceofthestudy..........................................................................................107.4.Analyticalmethod......................................................................................................117.5.Formulas....................................................................................................................127.6.Results.......................................................................................................................137.7.Conclusion.................................................................................................................157.8.Figures.......................................................................................................................168.MELTINGANDBOILINGTEMPERATURE........................................................................178.1.Guidelines..................................................................................................................178.2.Performanceofthetest.............................................................................................178.3.Analyticalconditions..................................................................................................188.4.Interpretation.............................................................................................................188.5.Results.......................................................................................................................198.6.Conclusion.................................................................................................................198.7.Figures.......................................................................................................................209.WATERSOLUBILITY..........................................................................................................229.1.Guidelines..................................................................................................................229.2.Reagents...................................................................................................................229.3.Performanceofthestudy..........................................................................................229.4.Analyticalmethod......................................................................................................239.5.Interpretation.............................................................................................................249.6.Results.......................................................................................................................259.7.Conclusion.................................................................................................................2510.FLASH-POINT.....................................................................................................................2610.1.Guidelines..................................................................................................................2610.2.Statement..................................................................................................................2611.FLAMMABILITYINCONTACTWITHWATER...................................................................2711.1.Guideline...................................................................................................................2711.2.Statement..................................................................................................................2712.PYROPHORICPROPERTIES............................................................................................2812.1.Guideline...................................................................................................................2812.2.Statement..................................................................................................................2813.EXPLOSIVEPROPERTIES................................................................................................2913.1.Guideline...................................................................................................................2913.2.Statement..................................................................................................................2914.AUTO-IGNITIONTEMPERATURE.....................................................................................3014.1.Guidelines..................................................................................................................3014.2.Performanceofthetest.............................................................................................3014.3.Interpretation.............................................................................................................3114.4.Results.......................................................................................................................3214.5.Conclusion.................................................................................................................32-Page2-T rans-1-chloro-3,3,3-trifluoropropene

NOT OX Project 492820 15. OXIDIZING PROPERTIES ...................... ........ .........................................................

. .......... 33 15.1. Guideline .......................................

. . ... ..... .. ....................................... ......... ................ 33 15.2. Statement ................................... .............. .................................................................

33 16. HYDROLYSIS
AS A FUNCTION OF PH .............................................................................

34 16.1. Guidelines

. .... .. ........................... ................ ..........................

. . .. ..................................

34 16.2. Statement ....... ... ..... ..... ..... ............

. .............. ........ ........................

.... ...... .......... .......... 34 17. ADSORPTION COEFFICIENT .................

.. .. .... .. .. ........ ....... .... .......... .............. .... ..............

.. 35 17.1. Guidelines .........................................

. .................

... .... ..... ... ..... ...............

. .............. ..... 35 17

. 2. QSAR .................................................

. . ............ .... .......... ........ .... ... ..... .......................

35 17.3

. Conclusion

... ....

. ..... ........... .....

. . ...

. .............. ..................................... ............ ............... 35 APPENDICES Appendix

I Certificate
of analysis ................ ................ .................................. ............... ............. 36 Appendix

II GLP certificate ........................................................................................................

37 -Page 3-T rans-1-chloro-3

, 3,3-trifluoropropene

NOT OX Project 492820 2. STATEMENT OF GLP COMPLIANCE NOTOX B.V., 's-Hertogenbosch, The Netherlands The study described

in this report has been correctly reported and was conducted

in compliance with: The Organization for Economic Cooperation and Development (OECD) Good Laboratory Practice Guidelines (1997)

. Which essentially conform to: The United States Food and Drug Administration Good Laboratory Practice Regulations. The United States Environmental Protection Agency Good Laboratory Practice Regulations

. The sponsor

is responsible for Good Laboratory Practice (GLP) compliance for all test substance information unless determined
by NOTOX

. NOTOX B.V

. Dr. K

. A. Oudhoff Study Director **~-..d\-.o~**

Dr
. lr. E. Baltussen Section Head Analytical

& Physical Chemistry -= Date:

. . O'::f··Ju.b··

·· ·29.JQ

. .. .. . .. .. . -Page 4-Trans-1-chloro-3,3,3-trifluoropropene

NOT OX Project 492820 3. QUALITY ASSURANCE STATEMENT NOTOX

B

. V ., 's-Hertogenbosch, The Netherlands This report was inspected by the NOT OX Quality Assurance Unit to confirm that the methods and results accurately and completely reflect the raw data

. The dates of Quality Assurance inspections are given below

. During the on-site process inspections, procedures applicable

to this type of study were inspected. The report

i ng date is the date of reporting to the Study Director. The QAU report was then forwarded
to the Test Facility Management. Type of Inspections Phase/Process Study Protocol Protocol Amendment

01 Protocol Amendment
02 Report Protocol Amendment
03 Process Analytical and physical chemistry Test Substance Handling Observations/Measurements Head of Quality Assurance C.J. Mitchell

B

. Sc. A/£¥# Date:

..

. ...
. J.. C..

. -:-J.ul::2D.b ·Page

5 • Start End Inspection Inspection Reporting date date date 28-Dec-09 28-Dec-09 28-Dec-09 25-Jan-10
25 - Jan-10 25-Jan-10 03-Feb-10 03-Feb-10 03-Feb-10 02-Mar-10 08-Mar-10 08-Mar-10 07-Jul-10

07 - Jul-10 07-Jul-10 11-Jan-10 18-Jan-10 21-Jan-10Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 4. SUMMARY The results of the physico-chemical properties
of the test substance are given below. Parameter Guideline(s) Result Comment Analytical method Sanco 3029 successfully validated Melting temperature

EC A

. 1 not observed
at OECD 102 -90 "C
( 183K) Boiling temperature ECA

. 2 19"C (292K)

at 1021 ± 1 hPa OECD 103 Water solubility ECA.6 1.90 g/1 stirring for 1, 3 and OECD 105

5 hours at 20"C by the flask method Flash-point ECA.9 technically not possible statement UN p . 32.4.1
to be performed ISO 2719 ASTM 093 Flammability (contact with EC A.12 not flammable statement water)
UNN

. 5 Pyrophoric properties

EC A.13 not pyrophoric statement UNN

. 3 Explosive properties

EC A

. 14 not explosive statement UN Test series
3 Auto-ignition temperature
EC A.15 380"C

at 986.8-

1 035.9 hPa DIN 51794 IEC 79-4 Oxidizing properties

EC A.17 not oxidizing statement UN0.2 -Page 6-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 Hydrolysis as a function of ECC.7 technically not possible statement pH OECD 111 to be performed Adsorption coefficient

EC C.19 log Koc: 2

. 16 OSAR OECD 121 -Page 7-Trans-1-chloro-3,3,3-trifluoropropene 5. INTRODUCTION 5.1. Preface Sponsor Study Monitor Test Facility Study Director Study Plan 5.2. Aim of the study

NOTOX Project 492820 Honeywell Fluorine Products Europe BV c/o Honeywell Specialty Materials Haasrode Research park 2105 Grauwmeer

1 3001 HEVERLEE Belgium Mr. T. Vink NOTOXB.V

. Hambakenwetering

7 5231 DD ·s-Hertogenbosch The Netherlands Dr. K.A. Oudhoff Start Completion

13 January 201 0 29June 2010 The aim of the study was to determine the following physico-chemical properties for T rans-1-chloro-3,3,3-trifluoropropene: • Validation
of an analytical method • Melting and boiling temperature • Water solubility • Flash-point • Flammability (contact with water) • Pyrophoric properties • Explosive properties • Auto-ignition temperature • Oxidizing properties • Hydrolysis as a function
of pH • Adsorption coefficient 5.3. Storage and retention of records and materials Records and materials pertaining
to the study includ

i ng protocol, raw data, specimens (except specimens requiring refrigeration
or freezing) and the final report are retained

in the NOT OX archives for a period of at least 2 years after finalization
of the report. After this period

, the sponsor will be contacted
to determine how the records and materials should be handled

. NOTOX will retain information concerning decisions made. Those specimens requiring refrigeration

or freezing will be retained by NOTOX for as long as the quality of the specimens permits evaluation but no longer than three months after finalization of the report. NOT OX will retain
a test substance sample until the expiry date, but no longer than 10 years after finalization
of the report

. After this period the sample will be destroyed. -Page 8-T rans-1-chloro-3

,3 ,3-trifluoropropene 6. MATERIALS 6.1. Test substance 6
. 1.1 Test substance Information Identification Structure Molecular formula Molecular weight CAS Number Description Batch Purity Test substance storage Stability under storage conditions Expiry date

NOTOX Project 492820 T rans-1-chloro-3,3

, 3 - trifluoropropene F)l H)=( F Cl
H C 3 H 2 CIF 3 130.49619 1 02687-65-0 Clear colourless liquid 41024-45 99.99 area% At room temperature

i n the dark Stable 09 December 2010 (allocated by NOTOX

, 1 year after receipt of the test substance) The certificate
of analysis
is shown in Appendix

I. 6.1.2 Study specific test substance Information Test substance handling 6.2. Electronic data capture The cylinder was placed inverted
in order to sample the test substance To keep the test substance liquefied, handling after sampling was performed on dry ice System control, data acquisition and data processing were performed using the following programmes

: - Empower version 5.00 (Waters, Milford, MA, USA) -Advantage Integrity Software version 2.2

. 1 (TA Instruments, New Castle

, DE, USA) Temperature and/or relative humidity and/or pressure during sample storage and/or performance

of the studies were monitored continuously using the following programme: - REES Centron Environmental Monitoring system version SOL
2

. 0 (REES Scientific, Trenton, NJ, USA) 6.3. List of deviations 6.3.1 List of protocol deviations There were no deviations from the protocol. 6.3.2 List of standard operating procedures deviations Any deviations from standard operating procedures (SOPs) were evaluated and filed in the study file

. There were no deviations from SOPs that affected the integrity of the study. -Page 9-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 7. VALIDATION OF AN ANALYTICAL METHOD 7.1. Guideline The study was based on the following guideline: European Commission: Guidance for Generating and Reporting Methods
of Analysis
in Support of Pre-registration Data Requirements
for Annex

II (Part A, section 4) and Annex Ill (Part A, section 5) of Directive 91/414, SANC0/3029/99
rev. 4 (11/07/00). 1.2. Reagents Water Acetonitrile

Tap water purified by a Milli-Q water purification system (Millipore, Bedford, MA, USA). VWR International, Leuven, Belgium or Labscan, Dublin, Ireland. All reagents were of analytical grade, unless specified otherwise. 7.3. Performance

of the study A gas chromatographic method with flame ionization detection with headspace injection (headspace GC-FID) for the quantitative analysis of the test substance

in water was developed. Validation
of the analytical method was performed for the following parameters: Specificity A test substance solution and blank accuracy sample were analysed by single injection. The analytical method was found to be specific

if the blank chromatogram showed no response for the test substance
or a response
of < 30% of the limit of quantification. Linearity Calibration solutions were analysed

in duplicate. The response
of the calibration solutions was correlated with concentration using regression analysis with a 1/concentration

2 weighting factor. A calibration curve with a coefficient
of correlation
(r) of > 0.99 and back calculated accuracies of the calibration solutions
in the range 85-115% was accepted. Accuracy and repeatability Accuracy samples were analysed by single injection into the analytical system. The analytical method was considered applicable for the determination

of the test substance

if the mean accuracy was in the range 70-11 Oo/o and the coefficient

of variation was **~** 20%. Limit of quantification The limit of quantification (LOQ) Is defined as the lowest concentration level at which an accuracy

in the range 70-110% and a repeatability **of~** 20% is demonstrated. The LOQ was obtained from the data of the accuracy- and repeatability test. Stabiljty of the analvtical system and end solutions Calibration solutions were injected throughout the validation sequence including the beginning and end. The analytical system and/or end solutions were found to be stable

if the coefficient
of variation on the responses
of the solutions was s 20%. Stability of stock solutions Stock solutions
of the test substance were stored at room temperature for at least 12 hours

. Additional calibration solutions were prepared and analysed by single injection

. The stock solutions were found to be stable

if the coefficient

of variation on the response factors of the calibration solutions prepared with fresh- and stored stock solutions was **~** 1 Oo/o. -Page10-Trans-1-chloro-3,3,3-trifluoropropene
n.
' ' "-._ **~** 20J 1 **~** 4J u: ?. .. J:

I I ' ·60-

t$.1S'C ' \ 0 Tef11l'!'alure ('C) I I NOTOX Project 492820 File: TA_NOTOX_Nl t2640 696t9 50

tOO **~·** VUD TA lnltNtWJW Figure

6 DSC curve of the test substance obtained with the second experiment. Ae: TA_NOTOX.NL t2640 8t92t **~j**

' - **~ ~j**  §. **~** -IOJ u:

j ;;; .,

1 J:

l

,. _ .. '0:..------- --\( ' -50i l **~j**

' - 11)0· -too

·50

b

tOO ....... Tef11ll!fature ('C)
l.kllwrsll Y.C. tO TA lnlln.mlfttt Figure

7 DSC curve of the test substance obtained with the third experiment. -Page 21
-T rans-1-chloro-3

,3 ,3-trifluoropropene

NOT OX Project 492820 9. WATER SOLUBILITY 9.1. Guidelines Organization for Economic Co-operation and Development (OECD), OECD Guidelines for the Testing of Chemicals no. 105: "Water Solubility", July 27, 1995. European Community (EC), EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.6: "Water Solubility", Official Journal of the European Union no. L 142, May 31, 2008. 9.2. Reagents Water

Tap water purified by a Milli-0 water purification system (Millipore, Bedford, MA, USA) Double distilled water Acetonitrile Waldeck, Munster-Roxel, Germany VWR International, Leuven, Belgium or Labscan, Dublin, Ireland. All reagents were of analytical grade, unless specified otherwise. 9.3. Performance

of the study Two methods are available for the determination

of the water solubility
of a test substance: 1 . Column elution method The method
is based on the elution of the test substance with water from a column which is charged with an inert support material, such as bare silica particles, coated with an excess of the test substance.

It can be applied to test substances with a water solubility
of < 1 o· 2 g/1. 2. Flask method The mass concentration

of the test substance

in aqueous solutions saturated with the test substance will be determined after stirring or shaking at a specific temperature
for a specific period of time

. The method can be used for test substances with a water solubility
of <= 1 o· 2 g/1. For the determination

of the water solubility
of a liquid test substance, only the flask method can be used. The column elution method
is not applicable for liquid substances. A preliminary test was carried out as a range-finding test prior to performance

of the flask method. The sample vessels were completely filled with the aqueous phase to avoid head space of the test substance. Preliminary test Test substance was stirred with double distilled water at a nominal concentration

of 6.3 g/1 at 20.2 ± 0.6"C for 21 hours. After stirring

it was observed that the excess of the test substance was evaporated from the water phase i.e. a small gas bell was observed

in the sample vial. The aqueous phase of test sample was
1 000 times diluted to obtain an end solution
of water containing 1% (v/v) acetonitrile and concentrations within the calibration range. -Page 22-Trans-1-chloro-3,3,3-trifluoropropene

NOT OX Project 492820 Main study Three aliquots of the test substance were weighed into separate glass vessels

. The glass vessels were completely filled with double distilled water to avoid head space of the test substance

. The weightings
of the test substance and the volume of double distilled water were selected
in order to obtain nominal concentrations

of approximately

5 gil

. The vessels were placed on a magnetic stirring device
in a climate room 20.1 ± 0.5 "C where the content of each flask will be stirred for 1 , 3 or 5 hours

. In each water sample after the stirring periods an excess of the liquid test substance was observed
at the bottom of the vial. Centrifugation

or filtration
to remove the excess of the test substance was not required since the excess of the test substance was phase separated from the water phase at the bottom of the vial. Subamples

of the clear aqueous phases were
1 000 times diluted to obtain an end solution of water con

t aining 1% (v/v) acetonitrile and concentrations within the calibration range

. The pH of each of the water mixtures was determined

. As the blank, the same volume of double distilled water was stirred for 5 hours. The blank solution was pretreated identically
as the water samples from the flask method

. 9.4. Analytical method 9.4.1 Analytical conditions Quantitative analysis was performed according
to the analytical method as validated

in Chapter

7 . 9.4.2 Preparation

of the calibration solutions Stock solutions Stock solutions
of the test substance were prepared

in acetonitrile

at concentrations

of 2050

- 6620 mgll

. Calibration solutions Solutions

in the concentration range of 5 - 100 mg/1 or 5 - 230 mg/1 were prepared

in acetonitrile from two stock solutions. The solutions were 100 times diluted with water
(1 00 1-11 spiked to 10 ml water) to obtain calibration solutions

in the concentration range of 0.05-

1 mg/1 or 0.05-2.3 mg/1. The end solution of the calibration solutions was water containing 1% (v/v) acetonitrile

. 9.4.3 Sample injections Calibration solutions were injected
in duplicate. Test samples were analysed by single injection. 9.4.4 Calibration curves Calibration curves were constructed using five or six concentrations

. For each concentration, two responses were used. Linear regression analysis was performed using the least squares method with a 1/concentration

2 weighting factor.
If necessary

a response was excluded from the curve since the calibration solution was spiked twice. The coefficient
of correlation

(r) was > 0
. 99 for each curve

. -Page 23-Trans-1-chloro-3,3,3-trifluoropropene 9.5. Interpretation 9.4.5 Specifications

NOTOX Project 492820 The water solubility
of a test substance

is specified by the saturation mass concentration

of the test substance

in water at a given temperature and is expressed

in g/1. 9.4.6 Formulas Response (R) Maximum difference Calibration curve Analysed concentration (CA) Peak area of the test substance [units] highest value -lowest value x 1 OO% mean value where: 'mean value'
is the mean of the highest and lowest value. where: CN = nominal concentration [mg/1] a slope [units x Vmg] b = intercept [units] where: d = dilution factor -Page 24-Trans-1-chloro-3

, 3,3-trifluoropropene

NOTOX Project 492820 9.6. Results 9 . 4.7 Preliminary test The content of test substance dissolved

in the water sample was 0 . 799 g/1. With the preliminary test it was found that the excess of the test substance was evaporated from the water phase after 21 hours of stirring (i.e

. a gas bell was observed

in the sample vial). Based on this the main study for the determination

of the water solubility was performed

at 1, 3, and 5 hours of stirring

. 9.4.8 Main study The results for the samples taken at 1, 3 and 5 hours are given in Table
7 . The maximum difference (MD) of the concentrations determined

in the 1, 3 and 5 hour test samples was < 15%. According

to the guidelines, the water solubility
of the test substance

is given as the mean value of the analysed concentrations

. A small response
at the retention time of the test substance was observed

in the blank treated sample. The maximum contribution

of the response
to the test samples based on peak area was 0.24%. Based to this it was considered that the response had no significant effect on the results of the test samples

. Table
7 Main study

• water solubility
of the test substance Stirring time Analysed Mean

MD

pH concentration [hours]

[gil]
[gil]

[%] 1 1.86

1
. 90
4.3

7.6 3 1.94

7

. 6 5

1
. 89

7
. 5 9.7. Conclusion The flask method with stirring times of 1, 3 and 5 hours was applied for the determination

of the water solubility of Trans-1-chloro-3,3,3-trifluoropropene

. The water solubility
of the tesl substance
at 20"C was 1.90 g/1

. The pH of the aqueous samples was 7.5- 7.6.
-Page 25-Trans-1-chloro-3,3,3-trifluoropropene10.FLASH-POINT10.1.GuidelinesEuropeanCommunity(EC),ECno.440/2008,PartA:MethodsfortheDeterminationofPhysico-ChemicalProperties,GuidelineA.9:"Flash-point",OfficialJournaloftheEuropeanUnionno.L142,May31,2008.UnitedNations(UN),UNno.ST/SG/AC.10/11/Rev.4:RecommendationsontheTransportofDangerousGoods,PartIll:ClassificationProcedures,TestMethodsandCriteriaRelatingtoExplosivesofClass3,Class4,Division5.1andClass9,Paragraph32.4.1:"Non-ViscousFlammableLiquids",2003.TheInternationalOrganizationforStandardization(ISO),ISOGuide2719:"DeterminationofFlashPoint-PenskyMartensClosedCupmethod",2002.ASTMInternational,ASTMD93:"StandardTestMethodsforFlashPointbyPensky-MartensClosedCupTester",December10,2002.10.2.StatementAccordingtotheguidancefortheimplementationofREACHtheflashpointisarelevantpropertyforliquidsanddoesnotneedtobedeterminedforsolidsorgasesatroomtemperature.Ifasubstanceisaliquid,theflash-pointshouldbedeterminedbyaclosed-cupmethod.Thetestsubstancehasaboilingtemperatureof19"C(seeChapter8)andisthereforevolatile(gas)atroomtemperature.Theclosed-cupmethodsavailableintheliteratureareonlyapplicableforthedeterminationofflashpointsofliquefiedsubstances.Itwasnotexpectedthatthetestsubstancehasaflashpointbelowitsboilingtemperature.Accordingtothis,itwastechnicallynotpossibletodeterminetheflash-pointofthetestsubstance.TheflammabilityofthegaseousphaseofthetestsubstanceaccordingtotheECA.11guidelinewasdeterminedbyChilworthTechnologyUKinstudyplannumberGLP/104383/A(NOTOXproject493726).WiththisstudyitwasdemonstratedthatTrans-1-chloro-3,3,3-trifluoropropeneis"notaflammablegas".-Page26-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 11. FLAMMABILITY

IN CONTACT WITH WATER 11.1. Guideline European Community (EC), EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.12: "Flammability (Contact with Water)", Official Journal of the European Union no. L 142, May 31, 2008. United Nations (UN), UN no. ST/SG/AC.1 0/11/Rev.4: Recommendations

on the Transport
of Dangerous Goods, Part Ill: Classification Procedures, Test Methods and Criteria Relating
to Explosives
of Class 3, Class 4, Division 5.1 and Class 9, Test N.5: "Test Method for Substances which in Contact with Water Emit Flammable Gases", 2003. United Nations (UN), UN no. ST/SG/AC.1 0/11/Rev.4: Recommendations

on the Transport
of Dangerous Goods, Appendix
6: "Screening Procedures", 2003. 11.2. Statement A test substance

ls considered flammable when
it ignites spontaneously

in contact with water, or evolves

a flammable gas at a minimum rate of C!: 1 litre/kg of the substance per hour

. The molecular structure
of the test substance

is shown
in Paragraph 6.1

. The test substance does not contain groups that might lead to ignition
in contact with water and/or to the evolution of a flammable gas. Moreover the test substance was dissolved

in water during the determination

of the water solubility (see Chapter 9). It demonstrated that the test substance does not ignite spontaneously

in contact with water. In conclusion, Trans-1-chloro-3,3,3-trifluoropropene

is considered 'not flammable'

in contact with water.
-Page 27-Trans-1-chloro-3,3,3-trifluoropropene

NOT OX Project 492820 12. PYROPHORIC PROPERTIES 12.1. Guideline European Community (EC), EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.13: "Pyrophoric Properties
of Solids and liquids", Official Journal of the European Union no. L 142, May 31, 2008. United Nations (UN), UN no. ST/SG/AC.1 0/11/Rev

. 4: Recommendations

on the Transport
of Dangerous Goods, Part Ill: Classification Procedures, Test Methods and Criteria Relating
to Explosives

of Class 3, Class 4, Division 5.1 and Class 9, Test N.3: "Test Method for Pyrophoric liquids, 2003. United Nations (UN), UN no. ST/SG/AC.1 0/11/Rev.4: Recommendations

on the Transport
of Dangerous Goods, Appendix
6: "Screening Procedures•, 2003. 12.2. Statement A test substance

is considered pyrophoric when
it ignites within five minutes
of coming
in contact with air. The molecular structure
of the test substance

is shown
in Paragraph 6.1. The test substance does not contain groups that might lead to Ignition
in contact with air. Moreover the test substance was handled under normal laboratory conditions during this project.

It demonstrated that the test substance does not ignite in contact with air. In conclusion, Trans-1-chloro-3,3,3-trifluoropropene

has no pyrophoric properties. -Page 28-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 13. EXPLOSIVE PROPERTIES 13.1. Guideline European Community (EC), EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.14: "Explosive Properties", Official Journal of the European Union no. L142, May 31, 2008. United Nations (UN), UN no. ST/SG/AC.10/11/Rev.4

: Recommendations

on the Transport
of Dangerous Goods, Part
1: Classification Procedures, Test Methods and Criteria Relating
to Explosives
of Class
1, Section 13: "Test Series 3". 2003. United Nations (UN), UN no. ST/SG/AC.10/11/Rev.4: Recommendations

on the Transport
of Dangerous Goods, Appendix
6: "Screening Procedures", 2003. 13.2. Statement A test substance

is considered explosive when

it can react to produce very rapid increases

in temperature

or pressure. The molecular structure
of the test substance

is shown
in Paragraph 6.1

. The test substance does not contain chemical groups which are associated with explosive properties. 9.4.9 Oxygen balance The test substance has no oxygen
in its molecular structure. According
to this, the oxygen balance was not determined In conclusion, Trans-1-chloro-3,3,3-trifluoropropene

has no explosive properties. ·Page 29-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 14. AUTO-IGNITION TEMPERATURE 14.1. Guidelines European Community (EC), EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.15: "Auto-Ignition Temperature (liquids and Gases)". Official Journal of the European Union no

. l142, May 31, 2008. Deutsches lnstitut fur Normung (DIN), DIN Guide 51794: "Determining the Ignition Temperature of Petroleum Products", May 2003. International Electrotechnical Commision (IEC), IEC standard 79-4: "Electrical Apparatus for Explosive Gas Atmospheres, Part 4, Method of Test for Ignition Temperature", 1975. 14.2. Performance

of the test The auto-ignition temperature

of the test substance was determined using
a commercially available auto-ignition temperature apparatus (Chilworth Technology, Southampton, UK). The method
is applicable

to test substances with an auto-ignition temperature

in the range 75"C

• 650"C. In every ignition test, liquefied test substance was introduced into a preheated test vessel using a volumetric pipette.

It was observed visually whether

a reaction (clearly perceptible flame and/or explosion)

of the sample within five minutes after introduction took place at the prevailing temperature. After each ignition test, any vaporizable components left in the flask were blown out with air (Air Products, Amsterdam, The Netherlands). To keep the test substance liquefied, the test substance container was kept on dry ice. 9.4.1 0 Preliminary test Starting at 200"C, every 20"C temperature rise

, 70 f.ll test substance was introduced into the test vessel until ignition of the test substance was first observed (i.e. at 420"C)

. Starting at 430"C, every
5 "C decreased,
70 f.ll test substance was tested until no ignition of the test substance has been observed. The temperature

at which ignition was last observed

is the estimated auto-ignition temperature. 9.4.11 Main study Three tests were performed. The sample volumes applied and temperature range of each test are given below. Starting at the "high temperature", every 2"C decreased, the prevailing volume of test substance was tested until no ignition of the test substance has been observed. The temperature

at which ignition was last observed

is the auto-ignition temperature

at the sample volume applied. This procedure was repeated with the other volumes until a minimum auto· ignition temperature for each test was obtained

. With these results the auto-ignition temperature of the test substance was determined. The atmospheric pressure during the performance

of the main study was between 986.8 and 1035.9 hPa.
-Page 30-T rans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 Test Volume Temperature ('C) run
high

low Test
1

70

408

394 100

393

377 120

394

382 Test 2
140

404

392 190

399

385 225

406

394 Test 3
150

409

399 175

399

387 300

408

400 14.3. Interpretation The degree of auto-ignitability

is expressed

in terms of the auto-ignition temperature. The auto- ignition temperature

is the lowest temperature

at which the test substance ignites when mixed with air under the conditions defined
in the test method. -Page 31
-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 14.4. Results 9.4.12 Preliminary test The estimated auto-ignition temperature was 400"C. 9.4.13 Main study The results of the main study on the determination

of the auto-ignition temperature

of the test substance are given in Table 8. Ignition of the test substance

w

i thin 5 minutes after introduction was observed with each sample volume and with each test a minimum auto-ignition temperature was obtained. The maximum deviation between the three values was< 20"C

. According
to the guidelines,
no further testing was required. The lowest temperature

at which ignition of the test substance occurred was 383"C. This temperature was rounded off to the nearest multiple of 5"C giving an auto-ignition temperature of the test substance

of 380"C. TableS Main study- auto-Ignition temperature

of the test substance Test Volume Auto-ignition Ignition delay Colour of the Minimum temperature

flame auto-ignition temperature M **["C~**
[sl

rcc1 1
70 400

4 orange/blue
383 100 383

7 orange/blue 120 388

5 orange/blue 2 140 398

3 orange/blue

391 190
391

5 orange/blue 225 400

4 orange/blue 3 150 405

4 orange/blue
393 175 393

4 orange/blue 300 406

3 orange/blue 14.5. Conclusion Commercially available auto-ignition temperatures apparatus was used for the determination

of the degree of auto-ignitability

of Trans-1-chloro-3,3,3-trifluoropropene. The test substance

is auto-ignitable with an auto-ignition temperature

at 986.8- 1035.9 hPa of 380"C

.
-Page 32-Trans-1-chloro-3,3

, 3-trifluoropropene

NOTOX Project 492820 15. OXIDIZING PROPERTIES 15

. 1. Guideline European Community (EC)

, EC no. 440/2008, Part A: Methods for the Determination

of Physico-Chemical Properties, Guideline A.21: "Oxidizing Properties (Liquids)", Official Journal of the European Union no. L 142, May 31, 2008

. United Nations (UN), UN no

. ST/SG/AC

. 10/11/Rev.4: Recommendations

on the Transport
of Dangerous Goods, Part Ill : Classification Procedures, Test Methods and Criteria Relating to Explosives
of Class 3, Class 4, Division 5.1 and Class 9, Test 0.2: "Test for Oxidizing Liquids

" , 2003

. United Nations (UN), UN no

. ST/SG/AC.1 0111/Rev

. 4: Recommendations

on the Transport
of Dangerous Goods

, Appendix

6: "Screening Procedures", 2003

. 15.2. Statement A test substance

is considered
an oxidizing substance when
a of the substance and cellulose

in a 1
: 1 ratio (by mass) spontaneously ignites or the mean pressure rise time of the mixture

is less than or equal to mean pressure rise time of a 1:1 reference mixture (by mass) of 65% aqueous nitric acid and cellulose The molecular structure
of the test substance

is shown in Paragraph

6

. 1. The test substance does not contain groups that act as an oxidizing agent. The halogen atoms that are present
in the molecular structure
of the test substance are chemically bonded to carbon or hydrogen

. I n conclusion, Trans-1-chloro-3,3,3-trifluoropropene

has no oxidizing properties. -Page 33-Trans-1-chloro-3,3,3-trifluoropropeneNOTOXProject49282016.HVDROLVSISASAFUNCTIONOFPH16.1.GuidelinesOrganizationforEconomicCo-operationand Development(OECD),OECDGuidelinesfortheTestingofChemicalsno.111:"HydrolysisasaFunctionofpH",April13,2004.EuropeanCommunity(EC),ECno.440/2008,PartC:MethodsfortheDeterminationofEcotoxicity,GuidelineC.7:"Degradation-AbioticDegradation:Hydrolysisas aFunctionofpH",OfficialJournaloftheEuropeanUnionno.L142,May31,2008.16.2.StatementAccordingtotheguidelines,thehydrolysispreliminarytestshouldbeconductedat50'Cfor5daystodeterminewhetheratestsubstanceishydrolyticallyunstable.Whenitisknownthatacompoundishydrolyticallyunstable,ahydrolysistestatthreetemperaturesintherangeof10-70'Cshouldbeconductedtodeterminethehydrolysisrateof25'C.Neitherthepreliminarytestnorthehydrolysistestcantechnicallybeperformedforthetestsubstanceaccordingtothefollowinginformation:Itisdemonstratedthatthetestsubstancehasaboilingtemperatureof19'C(seeChapterB)andevaporatesfromaqueoussampleswhenstirringfor21hoursat20'C(seeChapter9).Itistechnicallynotpossibletokeepthetestsubstanceinaqueoussolutionsfor5daysat50'Cand/orforarelativelylongperiodoftimeat10-70'C.Inaddition,noreadilyhydrolysablefunctionalgroups,suchasestersoramidesareinthemolecularstructureofthetestsubstance.·Page34-Trans-1-chloro-3,3,3-trifluoropropene

NOTOX Project 492820 17. ADSORPTION COEFFICIENT 17.1. Guidelines Organization
for Economic Co-operation and Development (OECD), OECD Guideline for the Testing of Chemicals no. 121

: "Estimation

of the Adsorption Coefficient (l<oc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLCr, January 22, 2001. European Community (EC), EC no
. 440/2008, Part C: Methods for the Determination

of Ecotoxicity, Guideline

C

. 19: "Estimation

of the Adsorption Coefficient (l<oc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC)", Official Journal of the European Union no. L 142, May 31, 2008. Technical Guidance document

in support of Commission Directive 93/67/EEC
on Risk Assessment tor new notified substances and Commission Regulation (EC) 1488/94 on Risk Assessment for existing substances. Part Ill Use of (Quantitative) Structure Activity Relationships ((Q)SARs), pages 532- 543. 17.2. QSAR The Koc of the test substance was determined using
a quantitative structure activity relationship (QSAR). Based on the water solubility
of the test substance, the compound

is classified as "nonhydrophobics"

. For this chemical class, the log Kocis given as
: logK

0 c =0

. 521ogP

0 w +1

. 02 The partition coefficient
of the test substance given as the log Pow is 2.2. The result was determined
by CERI Japan using
a HPLC method.
A copy of the report with study number 83219 was supplied by the sponsor and is archived

in the raw data. Using the value of 2.2, the log Koc value of the test substance

is 2.16 (l<oc

: 1.46 x 1 0
2 ) . 17.3. Conclusion A QSAR calculation was used for the determination

of the Koc of Trans-1-chloro-3,3,3-trifluoropropene. The Koc and log Koc value of the test substance was: 1<,..,

IOQ Koc Test substance 1.46x10

2 2.16 ·Page 35 •Trans·1·chloro·3,3,3·trifluoropropene Appendix

I CertHicate
of analysis Honeywell International Buffalo Research Laboratory 20 Peabody Street Buffalo, New York 14210 USA Material; HCF0-1233zd
(E) (E t-Chloro-3,3,3-triftuoropropene) SampleiD tt: LOT -41024-45 AR#495Ui Assay: 99.99area% Organic Impurities

In area % HCF0-1233zd
(Z) 0.0016 Others 0.0039 Certified by: John L. Welch Sr. Technical Manager Analytical **Service.~** lll/04/09 -Page 36 • NOTOX Project 492820

I I i I I I I I I I l lTrans-1-chloro-3,3,3-trlfluoropropene

NOTOX Project 492820 Appendix

II GLP certificate ENDORSEMENT
OF COMPLIANCE WITH THE OECO PRINCIPLES OF GOOD LABORATORY PRACTICE Pursuant
to the Netherlands GLP Compliance Monitoring Programme and according
to Directive 2004/9/EC, the confonnity with the OECD Principles
of GLP was assessed on 16- 20 February, 2009 at NOTOXB.V. Hambakenwetering

7 5231 DD 's Hertogenbosch It Is herewith confirmed that the afore-mentioned
test **fac~ity** is currently operating in compliance with the OECD Principles
of Good Laboratory Practice
in the following area of expertise

: Physical-chemical testing; Toxicity studies; Mutagenicity studies; Environmental toxicity studies on aquatic and terrestrial organisms; Studies on behaviour

in water, soil, and air, bioaccumulation; Residue studies; Analytical and clinical chemislly testing; Kinetic and metabolism studies, Safety Pharmacology. Manager GLP Compliance Monitoring Program Food and eor-mer Pnxlucl Safely Aulhcrily f:o/WA) Pltnses **~~~~~**

2, 2585N.. Den Haag Postbua 19506. 2600 CM Den Haag **The~** -Page 37-