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Guide to the Application of Genotyping to Tuberculosis Prevention and Control										Related Pages
										**CDC Tuberculosis Genotyping Laboratory Procedures**
Two genotyping laboratories have been funded through contracts with CDC to support the CDC Tuberculosis Genotyping Program by providing genotyping services to TB programs in the United States. TB programs can submit one isolate from each person with culture-positive tuberculosis within their jurisdictions. The genotyping laboratories will analyze isolates from current patients, but TB programs may request permission to submit selected isolates collected in the past. Isolates that are thought to be the result of false-positive cultures can be submitted, even though they may not result in a reported case. In special circumstances described in Chapter 5, *Developing a Tuberculosis Genotyping Program*, TB programs may submit additional isolates from the same patient.
The genotyping laboratories will use three genotyping methods: spoligotyping, MIRU analysis, and IS*6110*-based RFLP analysis, also known as fingerprinting. Spoligotyping and MIRU analysis are based on PCR. Together, these two methods will be referred to as the PCR genotyping tests. All submitted isolates will be analyzed by the first two methods; selected isolates will be analyzed with RFLP analysis. Descriptions of the technical aspects of these three methods were published recently (Barnes 2003).
Genotyping results will be reported to the TB program but not to the submitting laboratories, except when isolates represent suspected false-positive cultures. When the submitting laboratory suspects a false-positive culture, genotyping results from these submissions will be provided to both the TB program and the submitting laboratory.
							
					
						Page last reviewed:  September 1, 2012					Content source: 	Division of Tuberculosis Elimination, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention homeGuide to the Application of Genotyping to Tuberculosis Prevention and Control
Acknowledgments
Introduction to Tuberculosis Genotypingplus icon
Introduction to this Guide
Overview of Tuberculosis Genotyping
Overview of the CDC Tuberculosis Genotyping Program
TB Program Eligibility and Application Procedures
Tuberculosis Genotyping Case Studiesplus icon
Investigation of a Genotyping Cluster in a Low-Incidence State
Apparent Genotyping Cluster among Recent Immigrants from the Philippines
False-Positive Culture Investigation
Algorithm to Detect False-Positive Cultures
A Misdiagnosis that was Identified with Genotyping
Genotyping Used to Improve Contact and Cluster Investigations
Identification of Nontraditional Transmission Settings
Investigation of a Multistate Tuberculosis Outbreak
Homeless Shelter Outbreak Investigation
Using Genotyping to Evaluate the Impact of a Screening Program
CDC Tuberculosis Genotyping Laboratory Proceduresplus icon
Science behind Tuberculosis Genotyping
Description of Genotyping Methods
Submission of Isolates
Genotyping Results, Designation of Clusters, and Reporting
Suspected False-Positive Cultures
IS*6110*-based RFLP Analysis
Combining Genotyping and Epidemiologic Data to Improve Our Understanding of TB Transmissionplus icon
Definitions
Reading the Genotyping Laboratory Report
Comparing Genotyping and Epidemiologic Results from Two TB Patients
Comparing Genotyping and Epidemiologic Results from Three or More TB Patients
Clustering as a Surrogate Measure of Recent Transmission
Developing a Tuberculosis Genotyping Programplus icon
Developing a Written Plan
Initial Decisions
Establishing Procedures for:plus icon
Submitting Isolates
Receiving and Acting on Genotyping Results
Requesting RFLP Analysis
Submitting Additional Isolates from the Same Patient
Comparing Genotyping Results between Jurisdictions
False-Positive Cultures
Database Management and Quality Control
Special Issues
Applying Genotyping Results to Tuberculosis Control Practicesplus icon
Evaluating Matching Genotypes
Evaluating Nonmatching Genotypes
Suspected False-Positive Culture Investigations
Expanded Contact Investigations
Outbreak Investigations
Cluster Investigations
Monitoring Trends
Appendicesplus icon
Appendix A: Glossary and Abbreviations
Appendix B: References and Useful Resourcesplus icon
References Cited in the Guide
NTGSN Special Issue References
Information on Packaging and Shipping Infectious Substances
Core Steps of an Outbreak Investigation
Consultants Available to Offer Guidance To receive email updates about this page, enter your email address: Email Address			What's this?				Submit


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