# APPENDIX H. Supporting Data Used for the A quatic, Probabilistic Risk Assessment of Endosulfans

I. Data Used for Derivation of Species Sensitivity Distributions

Table H-1. The LC50 values used for the freshwater fish species sensitivity distribution in Figure 5.5.

Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * Anabas testudineus, Climbing perch 4 1.2 1.2 5326 Anguilla anguilla, Common eel 4 41 41 11055 Aplocheilichthys johnstonii, Tooth carp 1 2.57 2.57 15049 Barbus conchonius, Rosy barb 2 21.36 21.36 7230 Barbus sophore, Two spot barb, dotted barb 4 1.2 1.2 9276 Barilius bendelisis, Cyprinid fish 4 14.71 15.79 89782 Barilius bendelisis, Cyprinid fish 4 16.95 89782 Bidyanus bidyanus, Silver perch 4 2.3 2.35 5850 Bidyanus bidyanus, Silver perch 4 2.4 5850 Channa orientalis, Smooth-breasted snakefish 4 10.55 10.59 5736 Channa orientalis, Smooth-breasted snakefish 4 10.59 5736 Channa orientalis, Smooth-breasted snakefish 4 10.6 5736 Channa orientalis, Smooth-breasted snakefish 4 10.64 5736 Channa punctata, Snake-head catfish 4 0.16 2.91 15157 Channa punctata, Snake-head catfish 4 2.5 15157 Channa punctata, Snake-head catfish 7 3.011 72552 Channa punctata, Snake-head catfish 4 4.8 15157 Channa punctata, Snake-head catfish 4 6.6 15157 Channa punctata, Snake-head catfish 4 16 15157 Clarias batrachus, Walking catfish 7 3.011 70.3 72552 Clarias batrachus, Walking catfish 4 200 88885 Clarias batrachus, Walking catfish 4 576 89006 Cyprinus carpio, common carp 4 0.1 3.23 5850 Cyprinus carpio, common carp 4 5.2 17179 Cyprinus carpio, common carp 4 9.5 103230 Cyprinus carpio, common carp 4 22 71889 Gambusia affinis, Western mosquitofish 4 1.3 2.96 5806 Gambusia affinis, Western mosquitofish 4 2.3 5850 Gambusia affinis, Western mosquitofish 4 3.2 568 Gambusia affinis, Western mosquitofish 4 8 568 Heteropneustes fossilis, Indian catfish 4 7.4 12.94 10497 Heteropneustes fossilis, Indian catfish 4 7.7 10497 Heteropneustes fossilis, Indian catfish 4 7.7 10497 Heteropneustes fossilis, Indian catfish 4 7.8 10497 Heteropneustes fossilis, Indian catfish 4 9.1 10497



# H-1Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * Heteropneustes fossilis, Indian catfish 4 9.8 10497 Heteropneustes fossilis, Indian catfish 4 10 10497 Heteropneustes fossilis, Indian catfish 4 10.3 10497 Heteropneustes fossilis, Indian catfish 4 10.6 10497 Heteropneustes fossilis, Indian catfish 4 10.7 10497 Heteropneustes fossilis, Indian catfish 4 10.8 15179 Heteropneustes fossilis, Indian catfish 4 12.4 10497 Heteropneustes fossilis, Indian catfish 4 12.7 10497 Heteropneustes fossilis, Indian catfish 4 14.1 10497 Heteropneustes fossilis, Indian catfish 4 14.1 10497 Heteropneustes fossilis, Indian catfish 4 14.3 10497 Heteropneustes fossilis, Indian catfish 4 15.1 10497 Heteropneustes fossilis, Indian catfish 4 16.9 10497 Heteropneustes fossilis, Indian catfish 4 17 10497 Heteropneustes fossilis, Indian catfish 4 18.1 10497 Heteropneustes fossilis, Indian catfish 4 19.9 10497 Heteropneustes fossilis, Indian catfish 4 20.3 10497 Heteropneustes fossilis, Indian catfish 4 22.2 10497 Heteropneustes fossilis, Indian catfish 4 22.8 10497 Heteropneustes fossilis, Indian catfish 4 22.8 10497 Ictalurus nebulosus, Channel ca tfish 4 1.5 1.5 submitted Labeo rohita, Rohu 4 0.33 1.33 468 Labeo rohita, Rohu 4 1 468 Labeo rohita, Rohu 4 7.1 468 Lepomic macrochirus, Bluegill sunfish 4 1.2 1.93 submitted Lepomic macrochirus, Bluegill sunfish 4 1.7 submitted Lepomic macrochirus, Bluegill sunfish 4 2.08 submitted Lepomic macrochirus, Bluegill sunfish 4 3.3 submitted Macquaria ambigua, Yellowbelly, Golden Perch 4 0.3 0.39 5850 Macquaria ambigua, Yellowbelly, Golden Perch 4 0.5 5850 Macropodus cupanus, Paradise fish 4 9.61 ** 9.61 87644 Melanotaenia duboulayi, Eastern rainbow fish 4 0.5 1.97 5850 Melanotaenia duboulayi, Eastern rainbow fish 4 2.4 5850 Melanotaenia duboulayi, Eastern rainbow fish 4 2.5 5850 Melanotaenia duboulayi, Eastern rainbow fish 4 5 5850 Monopterus albus, Swamp or White Ricefield Eel 4 0.42 0.42 100663 Morone saxatilis, Striped bass 4 0.22 0.28 15472 Morone saxatilis, Striped bass 4 0.23 15472 Morone saxatilis, Striped bass 4 0.23 15472 Morone saxatilis, Striped bass 4 0.29 15472 Morone saxatilis, Striped bass 4 0.35 15472



# H-2Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * Morone saxatilis, Striped bass 4 0.43 15472 Nematolosa erebi, Bony bream 4 0.2 0.2 5850 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.17 0.69 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.24 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.26 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.26 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.27 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.29 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.3 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.32 9479 Oncorhynchus mykiss, Rainbow trou t,donaldson trout 4 0.37 submitted Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.41 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.42 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.49 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.4975 87973 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.63 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.69 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.69 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.693 87973 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.7 5850 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.75 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.79 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.8 9479 Oncorhynchus mykiss, Rainbow trou t,donaldson trout 4 0.83 submitted Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 0.89 9479 Oncorhynchus mykiss, Rainbow trou t,donaldson trout 4 1.1 submitted Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.21 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.3 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.34 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.372 87973 Oncorhynchus mykiss, Rainbow trou t,donaldson trout 4 1.5 submitted Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.6 5850 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.63 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 1.69 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 2.43 9479 Oncorhynchus mykiss, Rainbow trout,donaldson trout 4 3.2769 87973 Pimephales promelas, Fathead minnow 4 0.29 1.29 9479 Pimephales promelas, Fathead minnow 4 0.45 9479 Pimephales promelas, Fathead minnow 4 0.73 9479 Pimephales promelas, Fathead minnow 4 0.75 9479 Pimephales promelas, Fathead minnow 4 0.76 9479



# H-3Freshwater Fish Species Dur (da) LC50 (ug/L) SMAV Ref * Pimephales promelas, Fathead minnow 4 0.8 9479 Pimephales promelas, Fathead minnow 4 0.81 9479 Pimephales promelas, Fathead minnow 4 0.86 submitted Pimephales promelas, Fathead minnow 4 0.97 9479 Pimephales promelas, Fathead minnow 4 1 9479 Pimephales promelas, Fathead minnow 4 1.2 9479 Pimephales promelas, Fathead minnow 4 1.2 9479 Pimephales promelas, Fathead minnow 4 1.35 9479 Pimephales promelas, Fathead minnow 4 1.48 9479 Pimephales promelas, Fathead minnow 4 1.5 submitted Pimephales promelas, Fathead minnow 4 1.57 9479 Pimephales promelas, Fathead minnow 4 1.67 9479 Pimephales promelas, Fathead minnow 4 1.7 9479 Pimephales promelas, Fathead minnow 4 1.9 9479 Pimephales promelas, Fathead minnow 4 1.91 9479 Pimephales promelas, Fathead minnow 4 2.1 9479 Pimephales promelas, Fathead minnow 4 2.1 9479 Pimephales promelas, Fathead minnow 4 2.5 9479 Pimephales promelas, Fathead minnow 4 3.2 9479 Pimephales promelas, Fathead minnow 4 3.2 9479 Pimephales promelas, Fathead minnow 4 3.45 9479 Poecilia reticulata, Guppy 4 2.7 2.7 10646 Rasbora heteromorpha, Harlequinfish, red rasbora 4 0.2 0.2 5850 Schilbe mystus, Butter barbel, silver barbel 1 5.11 5.11 15049 Tilapia mossambica, Mozambique tilapia 4 6 6 9391 Tilapia nilotica, Nile tilapia 2 0.69 0.69 20421 Tilapia sparrmanii, Banded bream 1 7.35 7.35 15049 

### * 

## The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # labeled “submitted” refers to data submitted by the registrant, and can be found in Appendix E. ** Note: in the ECOTOX database this was listed with wrong units, original publication checked.



## Table H-2. The LC50 values used for the freshwater invertebrate species sensitivity distribution in Figure 5.6.

Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * Alonella sp., Water flea 2 0.2 0.2 786 Atalophlebia sp., Mayfly 2 13.6 13.60 73404 Atalophlebia sp., Mayfly 2 12.3 73404 Atalophlebia sp., Mayfly 2 12.3 73404 Atalophlebia sp., Mayfly 2 15.2 73404 Atalophlebia sp., Mayfly 2 12.7 73404 Atalophlebia sp., Mayfly 2 15.9 73404 Barytelphusa guerini, Freshwater crab 4 17780 17780 14955



# H-4Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * Bellamya dissimilis, Snail 4 1800 1800 17911 Brachionus calyciflorus, Rotifer 1 5150 5150 5702 Caridina laevis, Smooth Caridina 4 1.02 1.02 100785 Cheumatopsyche sp., Caddisfly 2 1.8 0.85 20012 Cheumatopsyche sp., Caddisfly 2 0.4 20012 Crocothemis erythraea, Dragonfly 1 395 395 12022 Culex fatigans, Mosquito 2 150 150 11147 Culex pipiens, Mosquito 1 66 66 4431 Cypria sp., Ostracod 2 0.9 0.9 786 Daphnia carinata, Water flea 2 205 205 16513 Daphnia longispina, Water flea 2 0.3 0.3 11147 Daphnia magna, Water flea 2 158 451.9 9479 Daphnia magna, Water flea 2 166 submitted Daphnia magna, Water flea 2 218 9479 Daphnia magna, Water flea 2 250 9479 Daphnia magna, Water flea 2 266 9479 Daphnia magna, Water flea 2 271 9479 Daphnia magna, Water flea 2 282 9479 Daphnia magna, Water flea 2 328 9479 Daphnia magna, Water flea 2 342 9479 Daphnia magna, Water flea 2 372 9479 Daphnia magna, Water flea 2 378 9479 Daphnia magna, Water flea 2 630 9479 Daphnia magna, Water flea 2 740 9479 Daphnia magna, Water flea 2 831.6 87973 Daphnia magna, Water flea 2 945.25 80944 Daphnia magna, Water flea 2 1174.1 87973 Daphnia magna, Water flea 2 1509.36 87973 Daphnia magna, Water flea 2 2077.6 87973 Diaptomus sp., Calanoid copepod 2 0.6 0.6 786 Eretes sticticus, Beetle 2 10 10 5182 Eucyclops sp., Cyclopoid copepod 2 0.1 0.1 786 Gammarus faciatus, Scud 4 6 6 submitted Gammarus lacustris, Scud 4 5.8 5.8 submitted Hyalella azteca, Scud 4 2.6865 4.39 87973 Hyalella azteca, Scud 4 5.643 87973 Hyalella azteca, Scud 4 5.586 87973 Hydra viridissima, Hydra 4 670 670 20380 Hydra vulgaris, Hydra 4 810 810 20380 Jappa kutera, Mayfly 4 2.3 1.3 62479 Jappa kutera, Mayfly 4 0.7 62479



# H-5Freshwater Invertebrate Species Dur (d) LC50 ug/L SMAV Ref * Jappa kutera, Mayfly 4 1.2 62479 Jappa kutera, Mayfly 4 1.8 62479 Lamellidens marginalis, Mussel 2 43410 43410 126 Lanistes carinatus, Snail 1 3400 3400 12022 Lymnaea luteola, Pond snail 4 60 60 103723 Lymnaea natalensis, Pond snail 2 4370 4370 11147 Macrobrachium rosenbergii, Giant river prawn 4 0.93 0.43 61993 Macrobrachium rosenbergii, Giant river prawn 4 0.2 61993 Moina micrura, Water flea 1 16.2 16.2 87649 Oziotelphusa senex, Crab 2 6200 6200 18025 Palaemonetes paludosus, Riveri ne grass shrimp 4 0.24 0.24 93458 Paramelita nigroculus, Amphipod 1 19.2 19.2 87478 Paratya australiensis, Shrimp 2 0.96 0.70 103213 Paratya australiensis, Shrimp 2 0.51 103213 Procambarus clarkii, Red swamp crayfish 4 24 24 12802 Pseudagrion sp., Dragonfly 1 46 46 12022 Pteronarcys californica, Stonefly 4 2.3 2.3 submitted Spicodiaptomus chilospinus, Calanoid copepod 2 40 40 5264 Tigriopus japanicus, Harpac ticoid copepod 4 69.93 69.9 104287 

## * The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # labeled “submitted” refers to data submitted by th e registrant, and can be found in Appendix E.



## Table H-3. The LC50 values used for the estuarine/marine fish species sensitivity distribution in Figure 5.5.

Estuarine/Marine Fish Species Dur (d) LC50 (ug/L) SMAV Ref * Cymatogaster aggregata, Shiner perch 4 1.1 1.08 3752 Cymatogaster aggregata, Shiner perch 4 1.06 2264 Cyprinodon variegatus, Sheepshead minnow 4 0.34 1.38 3740 Cyprinodon variegatus, Sheepshead minnow 4 0.6 3740 Cyprinodon variegatus, Sheepshead minnow 4 0.71 3740 Cyprinodon variegatus, Sheepshead minnow 4 0.83 3740 Cyprinodon variegatus, Sheepshead minnow 4 0.88 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.1 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.15 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.19 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.2 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.4 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.4 3740 Cyprinodon variegatus, Sheepshead minnow 4 1.61 3740 Cyprinodon variegatus, Sheepshead minnow 4 2.5 3740 Cyprinodon variegatus, Sheepshead minnow 4 2.7 3740 Cyprinodon variegatus, Sheepshead minnow 4 2.74 3740



# H-6Estuarine/Marine Fish Species Dur (d) LC50 (ug/L) SMAV Ref * Cyprinodon variegatus, Sheepshead minnow 4 2.81 3740 Cyprinodon variegatus, Sheepshead minnow 4 2.87 3740 Cyprinodon variegatus, Sheepshead minnow 4 3.45 3740 Fundulus heteroclitus, Mummichog 4 1.45 0.93 95846 Fundulus heteroclitus, Mummichog 4 1.82 3726 Fundulus heteroclitus, Mummichog 4 0.31 3726 Fundulus heteroclitus, Mummichog 4 0.91 73399 Lagodon rhomboides, Pinfish 4 0.3 0.3 submitted Leiostomus xanthurus, Spot 4 0.09 0.09 submitted Mugil cephalus, Striped mullet 4 0.38 0.38 submitted Morone saxatilis, Striped bass 4 0.1 0.1 submitted 

## * The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # labeled “submitted” refers to data submitted by th e registrant, and can be found in Appendix E.



## Table H-4. The LC50 values used for the estuarine/marine invertebrate species sensitivity distribution in Figure 5.6.

Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * Americamysis bahia, Opossum shrimp 4 0.24 0.78 3740 Americamysis bahia, Opossum shrimp 4 0.34 3740 Americamysis bahia, Opossum shrimp 4 0.36 3740 Americamysis bahia, Opossum shrimp 4 0.38 3740 Americamysis bahia, Opossum shrimp 4 0.46 3740 Americamysis bahia, Opossum shrimp 4 0.73 3740 Americamysis bahia, Opossum shrimp 4 0.75 3740 Americamysis bahia, Opossum shrimp 4 0.94 3740 Americamysis bahia, Opossum shrimp 4 1.04 3740 Americamysis bahia, Opossum shrimp 4 1.04 3740 Americamysis bahia, Opossum shrimp 4 1.12 3740 Americamysis bahia, Opossum shrimp 4 1.16 3740 Americamysis bahia, Opossum shrimp 4 1.29 3740 Americamysis bahia, Opossum shrimp 4 1.47 3740 Americamysis bahia, Opossum shrimp 4 1.52 3740 Americamysis bahia, Opossum shrimp 4 1.77 3740 Artemia parthenogenetica, Brine shrimp 1 1890.5 4071 20398 Artemia parthenogenetica, Brine shrimp 1 8768.5 20398 Artemia salina, Brine shrimp 1 2688.5 2689 20398 Brachionus plicatilis, Rotifer 1 5600 6432 12646 Brachionus plicatilis, Rotifer 1 6600 12646 Brachionus plicatilis, Rotifer 1 7200 12646 Callinectes sapidus, Blue crab 5-6 125 237 67696 Callinectes sapidus, Blue crab 6-8 450 18705 Cancer magister, Dungeness or edible crab 4 14.8 14.8 3752



# H-7Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * Crassostrea madrasensis, Oyster 4 14.13 14.13 14270 Crassostrea virginica, Oyster 4 0.45 0.45 submitted Dinophilus gyrociliatus, Archiannelid 4 933.5 1029 11940 Dinophilus gyrociliatus, Archiannelid 4 1135 11940 Gammarus palustris, Gammarid amphipod 4 0.43 0.48 51439 Gammarus palustris, Gammarid amphipod 4 0.54 51439 Litopenaeus stylirostris, Blue Shrimp 2 235 235 73317 Macrophthalmus erato, Mangrove crab 4 0.48 0.48 69820 Nanosesarma sp., Crab 4 31 31 18580 Palaemonetes pugio, Daggerblade grass shrimp 4 0.12 0.77 73399 Palaemonetes pugio, Daggerblade grass shrimp 4 0.18 3726 Palaemonetes pugio, Daggerblade grass shrimp 4 0.206 61089 Palaemonetes pugio, Daggerblade grass shrimp 4 0.28 3726 Palaemonetes pugio, Daggerblade grass shrimp 4 0.303 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.31 3726 Palaemonetes pugio, Daggerblade grass shrimp 4 0.337 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.39 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.513 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.563 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.583 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.62 61089 Palaemonetes pugio, Daggerblade grass shrimp 4 0.62 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.63 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.641 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.645 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.73 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.894 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.911 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.92 61089 Palaemonetes pugio, Daggerblade grass shrimp 4 0.96 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 0.96 18932 Palaemonetes pugio, Daggerblade grass shrimp 4 0.97 18932 Palaemonetes pugio, Daggerblade grass shrimp 4 1.01 95846 Palaemonetes pugio, Daggerblade grass shrimp 4 1.01 18932 Palaemonetes pugio, Daggerblade grass shrimp 4 1.31 submitted Palaemonetes pugio, Daggerblade grass shrimp 4 1.342 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 1.397 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 1.4 8613 Palaemonetes pugio, Daggerblade grass shrimp 4 1.99 61089 Palaemonetes pugio, Daggerblade grass shrimp 4 2.56 71986 Palaemonetes pugio, Daggerblade grass shrimp 4 117 71986



# H-8Estuarine/Marine Invertebrate Species Dur (d) LC50 (ug/L) SMAV Ref * Penaeus duorarum, Northern pink shrimp 4 0.04 0.04 submitted Penaeus sp., Penaeidean shrimp 2 0.18 0.24 3726 Penaeus sp., Penaeidean shrimp 2 0.31 3726 Sagitta sp., Chaetognaths 1 424 424 2658 Uca pugnax, Fiddler crab 4 790 790 submitted 

## * The Ref # refers to the ECOTOX reference number, the citation for this can be found in Appendix D. Ref # labeled “submitted” refers to data submitted by th e registrant, and can be found in Appendix E.



# II. Species Sensitivity Distribution Equations

Cumulative species sensitivity dist ribution (SSD) curves were created and evaluated in a manner similar to that of Carriger and Rand (2008) using a log-logistic distribution. Each data point reflects the geometric mean of th e acute toxicity values within a species. The formula used for calculating the SSD within each taxonomic group is:

X X



# e e p βα βα

+ +



# + = 1 (Equation 1)

where: p = cumulative % of species affected; X

= log 10 

# (species LC50); and

α and β = scale parameters (Warren-Hicks et al. 2002)

The linear form of the equation 1 is written as:

X p p βα += ⎟ ⎟ ⎠ ⎞ ⎜ ⎜ ⎝ ⎛

−

1 ln (Equation 2)

Source: Carringer and Rand (2008)

Based on the above information, parame ters log-logistic regression equations 

# were determined maximizing the R 2 

# between the observed and calculated LC50 values using the Microsoft ® 

# Excel “Solver” application ( Table H-5 ).

Table H-6. Parameters for the log-logistic regr ession models for acute toxicity for various taxonomic groups. Taxa α β

Freshwater Fish -0.952 2.434 Freshwater Invertebrates -1.313 0.937 Saltwater Fish 0.969 2.506 Saltwater Invertebrates -1.049 0.750 Aquatic Amphibians—4 da -2.221 1.525 Aquatic Amphibians—8 da -2.064 2.095



# H-9

# III. Joint Probability Curves Joint probability curves (JPC) we re created by using concentrati ons that encompassed the range of acute toxicity values for each SSD. For each concentration, the percentage of LC 50 

# values that were exceeded was calculated based on the SSD regression for each taxonomic group. Simultaneously, the number of exposure concentrati ons for each application scenario that were equal to or greater than each of these concentrations was determine d. This resulted in a series of data pairs for each exposure/taxa combination wh ich were used to generate the JPC for each taxonomic group. Results for selected LC 50 

# exceedence percentiles are shown in Tables H-6 through H-9.

Table H-6. Probabilities that estimated concentrations of endosulfans exceed a given percentage of species-mean LC50 values for freshwater fish. Percentage of Species-Mean LC50s Exceeded Crop Use Category Aerial or Ground Application Crop Exposure Scenario 5% 10% 50% 90% A IL Beans 0.99 0.98 0.08 0 G IL Beans 0.98 0.98 0.08 0 A WA Beans 0.58 0.08 0 0 Beans G WA Beans 0.08 0.03 0 0 A FL Citrus 0.97 0.94 0.14 0 Citrus* G FL Citrus 0.96 0.93 0.13 0 G CA Cotton 0.98 0.39 0 0 A S. TX Cotton 0.98 0.91 0.03 0 Cotton G S. TX Cotton 0.98 0.93 0.05 0 G CA Melon 0.31 0.08 0 0 Cucurbits G S. TX Melon 0.97 0.89 0.03 0 Eggplant* G CA Eggplant 0.01 0 0 0 A MI Cherry 0.98 0.92 0.02 0 Fruits A WA Orchards 0.11 0.05 0 0 Lettuce G CA Lettuce 0.89 0.64 0.01 0 A ME Potato 0.98 0.97 0.03 0 G ME Potato 0.98 0.97 0.02 0 A WA Potato 0.96 0.39 0 0 Potato* G WA Potato 0.84 0.15 0 0 Tobacco G NC Tobacco 0.97 0.71 0.01 0 G CA Tomato 0.08 0.01 0 0 A FL Tomato 0.98 0.95 0.03 0 A PA Tomato 0.99 0.98 0.05 0 Tomato* G PA Tomato 0.98 0.98 0.04 0 

## * = exposure scenarios illustrated in Section 5.2.2.3 

## Table H-7. Probabilities that estimated concentrations of endosulfans exceed a given percentage of species-mean LC50 values for freshwater invertebrates.

Percentage of Species-Mean LC50s Exceeded Crop Use Category Aerial or Ground Application Crop Exposure Scenario 5% 10% 50% 90% A IL Beans 0.99 0.99 0 0 G IL Beans 0.99 0.98 0 0 A WA Beans 0.98 0.84 0 0 Beans G WA Beans 0.96 0.16 0 0



# H-10

### Percentage of Species-Mean LC50s Exceeded Crop Use Aerial or Ground Crop Exposure Category Application Scenario 5% 10% 50% 90% A FL Citrus 0.98 0.98 0 0 Citrus* G FL Citrus 0.98 0.97 0 0 G CA Cotton 0.98 0.98 0 0 A S. TX Cotton 0.99 0.98 0 0 Cotton G S. TX Cotton 0.98 0.98 0 0 G CA Melon 0.97 0.51 0 0 Cucurbits G S. TX Melon 0.99 0.98 0 0 Eggplant* G CA Eggplant 0.77 0.02 0 0 A MI Cherry 0.99 0.99 0 0 Fruits A WA Orchards 0.99 0.31 0 0 Lettuce G CA Lettuce 0.97 0.95 0 0 A ME Potato 0.98 0.98 0 0 G ME Potato 0.98 0.98 0 0 A WA Potato 0.99 0.98 0 0 Potato* G WA Potato 0.99 0.94 0 0 Tobacco G NC Tobacco 0.99 0.98 0 0 G CA Tomato 0.97 0.14 0 0 A FL Tomato 0.99 0.99 0 0 A PA Tomato 0.99 0.99 0 0 Tomato* G PA Tomato 0.99 0.98 0 0 

## * = exposure scenarios illustrated in Section 5.2.2.3 

## Table H-8. Probabilities that estimated concentrations of endosulfans exceed a given percentage of species-mean LC50 values for estuarine/marine fish. Percentage of Species-Mean LC50s Exceeded Crop Use Category Aerial or Ground Application Crop Exposure Scenario 5% 10% 50% 90% A IL Beans 0.99 0.99 0.98 0.04 G IL Beans 0.99 0.98 0.98 0.04 A WA Beans 0.98 0.98 0.06 0 Beans G WA Beans 0.91 0.62 0.02 0 A FL Citrus 0.98 0.98 0.92 0.07 Citrus* G FL Citrus 0.98 0.97 0.92 0.06 G CA Cotton 0.98 0.98 0.19 0 A S. TX Cotton 0.99 0.99 0.8 0.02 Cotton G S. TX Cotton 0.98 0.98 0.88 0.03 G CA Melon 0.97 0.91 0.05 0 Cucurbits G S. TX Melon 0.98 0.98 0.78 0.02 Eggplant* G CA Eggplant 0.44 0.12 0 0 A MI Cherry 0.99 0.99 0.8 0.01 Fruits A WA Orchards 0.99 0.95 0.03 0 Lettuce G CA Lettuce 0.97 0.97 0.51 0.01 A ME Potato 0.98 0.98 0.96 0.01 G ME Potato 0.98 0.98 0.93 0.01 A WA Potato 0.99 0.99 0.19 0 Potato* G WA Potato 0.99 0.98 0.03 0



# H-11

### Tobacco G NC Tobacco 0.98 0.98 0.38 0 G CA Tomato 0.96 0.57 0.01 0 A FL Tomato 0.99 0.99 0.92 0 A PA Tomato 0.99 0.99 0.98 0.02 Tomato* G PA Tomato 0.99 0.98 0.96 0.02 

## * = exposure scenarios illustrated in Section 5.2.2.3



# Table H-9. Probabilities that estimated concentrations of endosulfans exceed a given percentage of species-mean LC50 values for estuarine/marine invertebrates.

Percentage of Species-Mean LC50s Exceeded Crop Use Category Aerial or Ground Application Crop Exposure Scenario 5% 10% 50% 90% A IL Beans 0.99 0.99 0 0 G IL Beans 0.99 0.99 0 0 A WA Beans 0.98 0.98 0 0 Beans G WA Beans 0.98 0.88 0 0 A FL Citrus 0.98 0.98 0 0 Citrus* G FL Citrus 0.98 0.98 0 0 G CA Cotton 0.98 0.98 0 0 A S. TX Cotton 0.99 0.99 0 0 Cotton G S. TX Cotton 0.99 0.98 0 0 G CA Melon 1 0.97 0 0 Cucurbits G S. TX Melon 0.99 0.98 0 0 Eggplant* G CA Eggplant 0.97 0.38 0 0 A MI Cherry 0.99 0.99 0 0 Fruits A WA Orchards 0.99 0.99 0 0 Lettuce G CA Lettuce 1 0.97 0 0 A ME Potato 0.98 0.98 0 0 G ME Potato 0.98 0.98 0 0 A WA Potato 0.99 0.99 0 0 Potato* G WA Potato 0.99 0.99 0 0 Tobacco G NC Tobacco 0.99 0.98 0 0 G CA Tomato 0.99 0.95 0 0 A FL Tomato 0.99 0.99 0 0 A PA Tomato 0.99 0.99 0 0 Tomato* G PA Tomato 0.99 0.99 0 0 

## * = exposure scenarios illustrated in Section 5.2.2.3



# H-12

# IV. References

Carriger, JF, Rand, GM. 2008. Aquatic risk assessme nt of pesticides in surface waters in and adjacent to the Everglades and Biscayne National Parks: II Probabilistic analyses. Ecotoxicology. 17:680-96.

Warren-Hicks, WJ, Parkhurst, BR and Butche r, JB. 2002. Methodology for Aquatic Ecological Risk Assessment. pp. 345-382 (in) L. Posthuma, GW Suter II and TP Traas. Species Sensitivity Distributions in Ecotoxicology. Lewis Publishers. 587 pp.

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