Request No.: 102570 Analyst: GJM

Charge No.: 20006799.0040

Notebook No.: 21400

Log

- in Date: 11/18/2008 

### Analysis Report

To: Al Arnold, Carol Grubb Dept./Loc.: RD3

- D207

From: G.J. Meixsell/D. J. Nagy /Ann Kotz Dept./Ext.: ETC

- GAS /12416/15438

Date: 1 2/ 0 8/08 Lab Name: Polymer Separations

Subject: Molecular Weight Analysis by High Resolution Low Mass GPC analysis

Sample No.: BA

- 100

Summary

Sample BA

- 100, the reaction of pthalic acid and poly(ethylene glycol 200)

, w as submitted for molecular weight

distribution via Low Mass Gel Permeation Chromatography (LW

- GPC) analysis to determine if the sample adheres

to S chedule 9 polymer regulations

. The s ample was analyzed using a Waters Corporation Alliance 2 795 HPLC

with THF as a mobile phase @ 35 o C 1mL/min; sample was diluted to approximately 0. 4 %wt in fresh THF prior to

separation. Sample result is relative to a poly(ethylene glycol) calibration curve ranging from 62 daltons up to

1,900 K daltons. In addition to GPC, MALD/I analysis was performed to provide additional support information

regarding structure and mass assignment. Molecular weight and molecular weight distribution results are

summarized below.

Results Summary Table #1

Sample Id Mn Mw Slice MW Cumulative

% >348 d Slice MW Cumulative

% <1000d Slice MW Cumulative

% <500d

BA

- 100 (GPC Result 317 353 51.95 % * 99.89% 92.28%

BA

- 100 MALD/I

Result 419 464 74.3 % **

*see attached xls file containing slice log Mw information

**see ppt file containing MALD/I dataResults and Discussion :

The GPC data exhibits 50% of the distribution’s mass is greater than 348 daltons which indicates that the sample is

polymeric in accordance to S chedule 9. Furthermore, no more than 20% by weight of molecules are of the same

molecular weight by GPC separation . Additionally, the MALD/I data indicates that greater than 74.3% of the

distribution is greater than 348daltons with average repeat units of 5.1 and 5.5 EO/molecule ; the addition of the

repeat EO unit can be easily observed on the MALD/I plot. See tables and plots below for details.MALD/I Summarized S lice Component Results

GPC Run Conditions:

Instruments: Waters Alliance 2 7 95 Separation Module

Column: Waters Styragel HR 0.5, 300 x 7

. 8 mm

Detector Waters 2414 DRI @ 35C

Mobile Phase: Isocratic 100% THF ACS grade

Calibration Curve Narrow PEG Standards

Flow Rate: 1 ml/minute

Injection Volume: 100 μL

Data System: Waters Empower SoftwareDescription of Low

- Mass GPC

- Molecular Weight Report

Sample Information:

Sample identification, file name, and other administrative information are given at the top of page of this report.

Molecular Weights:

Below the header information on page 1 is a summary of all the appropriate molecular weight distribution data.

Molecular weight averages are reported relative to a poly(styrene) calibration. The most commonly used molecular

weights are the number

- average (Mn) and weight

- average (Mw). The polydispersity is the ratio of Mw / Mn and is a

measure of the broadness of the molecular weight distribution.

Molecular Weight Distribution:

In the middle of the page is the differential molecular weight distribution graph of weight

- fraction versus

log(molecular weight). The x

- axis usually covers several orders of magnitude in molecular weight, such as from 100

(10E2.0) to 100,000 (10E5.0) daltons. Overlaid wi th the differential molecular weight distribution curve is the

cumulative molecular weight distribution plot of cumulative weight

- fraction versus log(molecular weight). This type

of plot is used to determine weight percent less than or greater than a speci fic molecular weight. The y

- axis is

displayed in the range from 0 to 100 and the x

- axis covers the same range in mass as the differential molecular

weight distribution curve.

GPC Chromatogram:

On the bottom of the page is the raw GPC chromatogram of diffe rential refractive index (DRI) signal versus elution

time is shown on page 1. Detector signal is plotted as a function of elution time in minutes. Materials with

decreasing molecular weight elute with increasing retention time. The narrow peak on the chrom atogram is toluene,

which is added to each sample as an internal standard. The dotted lines are the areas under the peak area being

integrated.