13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J11030167

Lab Contact: Jason C Perkins

Date: 3/23/2011

Customer Address: 253 Plant Allen Road

Customer Name(s): Don Scruggs, Robbin Jolly, Ray Lidke, Bill Kennedy

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Test Burn #3 - Day 5

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with an "X" or "1" indicate a deviation from the method quality 

system or quality control requirement. All results are reported on a dry weight basis unless otherwise noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Analytical Laboratory 

Page 1 of 25
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2011005282 ALLEN 06-Mar-11 6:15 AM Chris Williams FGD Purge Eff 

2011005283 ALLEN 06-Mar-11 7:51 AM Chris Williams EQ Tank Eff 

2011005284 ALLEN 06-Mar-11 7:55 AM Chris Williams BioReactor 1 Inf 

2011005285 ALLEN 06-Mar-11 8:00 AM Chris Williams BioReactor 2 Inf 

2011005286 ALLEN 06-Mar-11 8:04 AM Chris Williams BioReactor 2 Eff 

2011005287 ALLEN 06-Mar-11 3:45 PM DANIEL HUDDLESTON BioReactor 1 Inf BLANK 

2011005288 ALLEN 06-Mar-11 3:55 PM DANIEL HUDDLESTON BioReactor 2 Inf BLANK 

2011005289 ALLEN 06-Mar-11 3:50 PM DANIEL HUDDLESTON BioReactor 2 Eff BLANK 

2011005290 ALLEN 06-Mar-11 8:10 AM Chris Williams Filter Blk 

9 Total Samples

Analytical Laboratory 

Page 2 of 25
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

The Vendor Laboratories have been qualified by the 

Analytical Laboratory

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis Test Case Narratives

Analytical Laboratory QC Report Chain of Custody

Reviewed By: DataBase Administrator Date: 3/23/2011

Yes

Electronic Data Deliverable (EDD) Sent Separately

Analytical Laboratory 

Page 3 of 25
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11030167

2011005282

Collection Date: 06-Mar-11 6:15 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 60.8 ug/L EPA 245.1 2.5 17-Mar-11 10:44 MHH7131

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 1 ug/L EPA 245.1 1 17-Mar-11 10:46 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 241 mg/L EPA 200.7 0.5 14-Mar-11 13:55 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 494 ug/L EPA 200.8 10 11-Mar-11 00:37 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 169 ug/L EPA 200.8 10 14-Mar-11 15:13 KRICHAR

Chromium (Cr) 168 ug/L 10 EPA 200.8 14-Mar-11 15:13 KRICHAR

Copper (Cu) 182 ug/L 10 EPA 200.8 14-Mar-11 15:13 KRICHAR

Nickel (Ni) 332 ug/L 10 EPA 200.8 14-Mar-11 15:13 KRICHAR

Selenium (Se) 1100 ug/L 10 EPA 200.8 14-Mar-11 15:13 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 14-Mar-11 15:13 KRICHAR

Zinc (Zn) 454 ug/L 20 EPA 200.8 14-Mar-11 15:13 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011005283

Collection Date: 06-Mar-11 7:51 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 47.7 ug/L EPA 245.1 2.5 17-Mar-11 11:07 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 152 mg/L EPA 200.7 0.5 14-Mar-11 13:59 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 752 ug/L EPA 200.8 10 11-Mar-11 00:41 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 118 ug/L EPA 200.8 10 14-Mar-11 15:07 KRICHAR

Chromium (Cr) 117 ug/L 10 EPA 200.8 14-Mar-11 15:07 KRICHAR

Copper (Cu) 141 ug/L 10 EPA 200.8 14-Mar-11 15:07 KRICHAR

Nickel (Ni) 238 ug/L 10 EPA 200.8 14-Mar-11 15:07 KRICHAR

Selenium (Se) 959 ug/L 10 EPA 200.8 14-Mar-11 15:07 KRICHAR

Analytical Laboratory 

Page 4 of 25
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11030167

2011005283

Collection Date: 06-Mar-11 7:51 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Silver (Ag) < 10 ug/L EPA 200.8 10 14-Mar-11 15:07 KRICHAR

Zinc (Zn) 299 ug/L 20 EPA 200.8 14-Mar-11 15:07 KRICHAR

2011005284

Collection Date: 06-Mar-11 7:55 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 69.4 mg/L EPA 200.7 0.5 14-Mar-11 14:03 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 1040 ug/L EPA 200.8 10 11-Mar-11 00:44 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L EPA 200.8 10 14-Mar-11 15:03 KRICHAR

Chromium (Cr) < 10 ug/L 10 EPA 200.8 14-Mar-11 15:03 KRICHAR

Copper (Cu) < 10 ug/L 10 EPA 200.8 14-Mar-11 15:03 KRICHAR

Nickel (Ni) 12.8 ug/L 10 EPA 200.8 14-Mar-11 15:03 KRICHAR

Selenium (Se) 1130 ug/L 10 EPA 200.8 14-Mar-11 15:03 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 14-Mar-11 15:03 KRICHAR

Zinc (Zn) < 20 ug/L 20 EPA 200.8 14-Mar-11 15:03 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011005285

Collection Date: 06-Mar-11 8:00 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 48.9 mg/L EPA 200.7 0.5 14-Mar-11 14:07 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L EPA 200.8 10 14-Mar-11 14:57 KRICHAR

Chromium (Cr) < 10 ug/L 10 EPA 200.8 14-Mar-11 14:57 KRICHAR

Copper (Cu) < 10 ug/L 10 EPA 200.8 14-Mar-11 14:57 KRICHAR

Nickel (Ni) 11.5 ug/L 10 EPA 200.8 14-Mar-11 14:57 KRICHAR

Analytical Laboratory 

Page 5 of 25
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11030167

2011005285

Collection Date: 06-Mar-11 8:00 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Selenium (Se) < 10 ug/L EPA 200.8 10 14-Mar-11 14:57 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 14-Mar-11 14:57 KRICHAR

Zinc (Zn) < 20 ug/L 20 EPA 200.8 14-Mar-11 14:57 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011005286

Collection Date: 06-Mar-11 8:04 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 52.0 mg/L EPA 200.7 0.5 14-Mar-11 14:11 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L EPA 200.8 5 14-Mar-11 14:53 KRICHAR

Chromium (Cr) < 5 ug/L 5 EPA 200.8 14-Mar-11 14:53 KRICHAR

Copper (Cu) < 5 ug/L 5 EPA 200.8 14-Mar-11 14:53 KRICHAR

Nickel (Ni) < 5 ug/L 5 EPA 200.8 14-Mar-11 14:53 KRICHAR

Selenium (Se) < 5 ug/L 5 EPA 200.8 14-Mar-11 14:53 KRICHAR

Silver (Ag) < 5 ug/L 5 EPA 200.8 14-Mar-11 14:53 KRICHAR

Zinc (Zn) < 10 ug/L 10 EPA 200.8 14-Mar-11 14:53 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011005287

Collection Date: 06-Mar-11 3:45 PM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011005288

Collection Date: 06-Mar-11 3:55 PM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Analytical Laboratory 

Page 6 of 25
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11030167

2011005288

Collection Date: 06-Mar-11 3:55 PM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011005289

Collection Date: 06-Mar-11 3:50 PM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011005290

Collection Date: 06-Mar-11 8:10 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

DISSOLVED METALS BY ICP-MS

Selenium (Se) 2.60 ug/L EPA 200.8 2 11-Mar-11 00:48 KRICHAR

Analytical Laboratory 

Page 7 of 25
 

March 21, 2011 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen – FGD Alternative Fuels Test Burn #3 - Daily) (LIMS # J11030167) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on March 8, 2011. The samples were received on March 9, 2011 in a 

sealed cooler at -0.4o

C. Selenium speciation analysis was performed via ion 

chromatography inductively coupled plasma dynamic reaction cell mass spectrometry 

(IC-ICP-DRC-MS). Any analytical issues associated with the analysis are addressed in 

the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

Analytical Laboratory 

Page 8 of 25
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen – FGD Alternative Fuels Test Burn #3 - Daily) (LIMS # J11030167) 

March 21, 2011 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation 

and Consulting) were submitted for selenium speciation analysis on March 8, 2011. 

The samples were received on March 9, 2011 in a sealed container at -0.4o

C. 

The samples were received in a laminar flow clean hood void of trace metals 

contamination and ultra-violet radiation. Upon reception, the samples were 

designated discrete sample identifiers. An aliquot of each sample was filtered 

(0.45μm) and these filtrates were stored in a secure, monitored cryofreezer 

(maintained at a temperature of -80oC) until selenium speciation analysis could be 

performed via ion chromatography inductively coupled plasma dynamic reaction cell 

mass spectrometry (IC-ICP-DRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free 

from trace metals contamination. All applied water for dilutions and sample 

preservatives are monitored for contamination to account for any biases associated 

with the sample results. 

Selenium Speciation Analysis by IC-ICP-DRC-MS Prior to analysis, an aliquot of 

each sample was filtered with a syringe filter (0.45μm) and injected directly into a 

sealed autosampler vial. No further sample preparation was performed as any 

chemical alteration of the samples may shift the equilibrium of the system resulting in 

changes in speciation ratios. 

Analytical Laboratory 

Page 9 of 25
3. Sample Analysis 

All sample analysis is precluded by a minimum of a five-point calibration curve 

spanning the entire concentration range of interest. Calibration curves are performed 

at the beginning of each analytical day. All calibration curves, associated with each 

species of interest, are standardized by linear regression resulting in a response factor. 

All sample results are instrument blank corrected to account for any operational 

biases associated with the analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source 

standards which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing 

calibration verification standards (CCV) and continuing calibration blanks (CCB) at a 

minimal interval of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-DRC-MS All samples for selenium 

speciation analysis were analyzed by ion chromatography inductively coupled plasma 

dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS) on March 11, 2011. An 

aliquot of each sample is injected onto an anion exchange column and mobilized by a 

basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio 

frequency (RF) plasma where energy-transfer processes cause desolvation, 

atomization, and ionization. The ions are extracted from the plasma through a 

differentially-pumped vacuum interface and travel through a pressurized chamber 

(DRC) containing a specific reactive gas which preferentially reacts with interfering 

ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions 

transmitted through the mass analyzer and the resulting current is processed by a data 

handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went very well and no analytical issues were encountered. All 

quality control parameters associated with these samples were within acceptance 

limits. 

The estimated method detection limits (eMDLs) for selenite, selenate, and 

selenocyanate are generated from replicate analyses of the lowest standard in the 

calibration curve. Not all selenium species are present in preparation blanks; 

therefore, eMDL calculations based on preparation blanks are artificially biased low. 

Analytical Laboratory 

Page 10 of 25
The eMDL for methylseleninic acid and selenomethionine is calculated from the 

average eMDL of selenite, selenate, and selenocyanate. The calibration does not 

contain methylseleninic acid or selenomethionine due to impurities in these standards 

which would bias the results for other selenium species. 

The selenocyanate recoveries for the matrix spike and matrix spike duplicates 

performed on the sample identified as Batch QC were below the established control 

limit of 75% (0.0%). The selenite recoveries for the matrix spike and matrix spike 

duplicate were 188.3% and 188.2%, respectively. The low selenocyanate and the 

elevated selenite recoveries are attributed to the sample matrix inducing oxidation of 

selenocyanate to selenite. The ambient sample concentrations support the oxidative 

nature of the samples since only oxidized selenium species were present. The quality 

control discrepancies were a function of the sample matrix; therefore, no corrective 

action was required. 

If you have any questions or concerns regarding this report, please feel free to contact 

me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Analytical Laboratory 

Page 11 of 25
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unkn

Spec

FGD Purge Eff 29.2 429 ND (<3.0) ND (<3.0) ND (<3.0) 0 BioReactor 1 Inf 7.09 987 ND (<0.76) ND (<0.75) 0.85 0 

BioReactor 2 Inf 2.74 ND (<0.90) ND (<0.76) ND (<0.75) ND (<0.75) 0 BioReactor 2 Eff 1.03 ND (<0.90) ND (<0.76) ND (<0.75) ND (<0.75) 0 

All results reflect the applied dilution and are reported in μg/LND = Not detected at the applied dilutionSeCN = SelenocyanateMeSe(IV) = Methylseleninic acidSeMe = SelenomethionineUnknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MSn = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen - FGD Alternative Fuels Test Burn #3 - Daily

Contact: Jay Perkins

Date: March 21, 2011

LIMS #J11030167
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 5Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.60 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.018 0.90 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.76 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.75 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.75 eMDL = Estimated Method Detection Limit*Please see narrative regarding eMDL calculationsQuality Control Summary - Certified Reference MaterialsAnalyte (μg/L) CRM True Value Result RecoverySe(IV) ICV 9.57 9.59 100.2Se(VI) ICV 9.48 9.05 95.4SeCN ICV 8.92 8.87 99.5MeSe(IV) ICV 6.47 5.73 88.6SeMe ICV 9.32 8.42 90.4

Selenium Speciation Results for Duke Energy

Project Name: Allen - FGD Alternative Fuels Test Burn #3 - Daily

Contact: Jay Perkins

LIMS #J11030167

Date: March 21, 2011

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPDSe(IV) Batch QC 1.30 1.10 1.20 16.4Se(VI) Batch QC ND (<0.90) ND (<0.90) NC NCSeCN Batch QC ND (<0.76) ND (<0.76) NC NCMeSe(IV) Batch QC ND (<0.75) ND (<0.75) NC NCSeMe Batch QC ND (<0.75) ND (<0.75) NC NCND = Not detected at the applied dilutionNC = Value was not calculated due to one or more concentrations below the eMDL Quality Control Summary - Matrix Spike/ Matrix Spike DuplicateAnalyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPDSe(IV) Batch QC 278.0 524.5 188.3 278.0 524.5 188.2 0.0Se(VI) Batch QC 252.3 256.1 101.5 252.3 256.6 101.7 0.2SeCN Batch QC 228.8 0.0 0.0* 228.8 0.0 0.0* 0.0*The low recovery is attributed to matrix induced species conversion

Selenium Speciation Results for Duke Energy

Project Name: Allen - FGD Alternative Fuels Test Burn #3 - Daily

Contact: Jay Perkins

LIMS #J11030167

Date: March 21, 2011

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC
Analytical Laboratory 

Page 15 of 25

Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic 

Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 17 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1111023-01 FGD Wastewater Sample 03/06/2011 03/09/2011

BioReactor 1 Inf Hg Blk 1111023-02 DIW Field Blank 03/06/2011 03/09/2011

BioReactor 2 Inf 1111023-03 FGD Wastewater Sample 03/06/2011 03/09/2011

BioReactor 2 Inf Hg Blk 1111023-04 DIW Field Blank 03/06/2011 03/09/2011

BioReactor 2 Eff 1111023-05 FGD Wastewater Sample 03/06/2011 03/09/2011

BioReactor 2 Eff Hg Blk 1111023-06 DIW Field Blank 03/06/2011 03/09/2011

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 03/11/2011 03/14/2011 B110373 1100161

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 18 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Fraction Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1111023-01 Hg FGD Wastewater T 273 7.58 20.2 ng/L B110373 1100161

BioReactor 1 Inf Hg Blk

1111023-02 Hg DIW T 0.15 U 0.15 0.41 ng/L B110373 1100161

BioReactor 2 Eff

1111023-05 Hg FGD Wastewater T 11.1 0.29 0.79 ng/L B110373 1100161

BioReactor 2 Eff Hg Blk

1111023-06 Hg DIW T 0.15 U 0.15 0.41 ng/L B110373 1100161

BioReactor 2 Inf

1111023-03 Hg FGD Wastewater T 22.4 0.59 1.57 ng/L B110373 1100161

BioReactor 2 Inf Hg Blk

1111023-04 Hg DIW T 0.15 U 0.15 0.41 ng/L B110373 1100161

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 19 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Analyte Native Spike Result Units REC & Limits RPD & Limits

Batch: B110373

Method: EPA 1631

Lab Matrix: Water

Sample

B110373-SRM1 Certified Reference Material (1111049, NIST 1641d 1000x dilution)

Reference Hg 15.68 15.35 ng/L 98% 85-115

B110373-MS1 Matrix Spike (1111021-03)

Matrix Spike Hg 30.02 141.2 174.0 ng/L 102% 71-125

B110373-MSD1 Matrix Spike Duplicate (1111021-03)

Matrix Spike Dup Hg 30.02 136.3 178.2 ng/L 109% 71-125 242%

Method Blanks & Reporting Limits

Batch: B110373

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B110373-BLK1 0.05 ng/L

B110373-BLK2 0.05 ng/L

B110373-BLK3 0.04 ng/L

B110373-BLK4 0.16 ng/L

Average: 0.08 Standard Deviation: 0.06 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 20 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Method: EPA 1631

Sequence: 1100161

Instrument: THG-10

Date: 03/14/2011

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1100161-IBL1 4.06 pg of Hg

1100161-IBL2 3.56 pg of Hg

1100161-IBL3 5.41 pg of Hg

1100161-IBL4 4.42 pg of Hg

1100161-CAL2 100.0 99.70 pg of Hg 100%

1100161-CAL3 500.0 500.9 pg of Hg 100%

1100161-CAL4 2500 2480 pg of Hg 99%

1100161-CAL5 10000 9684 pg of Hg 97%

1100161-ICV1 1568 1535 pg of Hg 98% 85-115

1100161-CCB1 12.4 pg of Hg

1100161-CAL6 25.00 26.09 pg of Hg 104%

1100161-CCV2 500.0 469.2 pg of Hg 94% 77-123

1100161-CCV3 500.0 471.4 pg of Hg 94% 77-123

1100161-CCV4 500.0 508.1 pg of Hg 102% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 21 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1111023-01 Report Matrix: FGD Wastewater

Sample: BioReactor 1 Inf Sample Type: Sample Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71092380

10

Cardboard 

Box

Bottle FLPE Hg-T 250-mL None N/A

Lab ID: 1111023-02 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71138980

50

Cardboard 

Box

Bottle FLPE Hg-T 250-mL None N/A

Lab ID: 1111023-03 Report Matrix: FGD Wastewater

Sample: BioReactor 2 Inf Sample Type: Sample Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Cardboard 

Box

Bottle FLPE Hg-T 250 mL None N/A

Lab ID: 1111023-04 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Cardboard 

Box

Bottle FLPE Hg-T 250 mL None N/A

Lab ID: 1111023-05 Report Matrix: FGD Wastewater

Sample: BioReactor 2 Eff Sample Type: Sample Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Cardboard 

Box

Bottle FLPE Hg-T 250 mL. None N/A

Lab ID: 1111023-06 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 03/09/2011

Collected: 03/06/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Cardboard 

Box

Bottle FLPE Hg-T 250-mL None N/A

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 22 of 25
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cardboard Box

Tracking No: 4726 7965 8274 via FedEx

Temperature: ambient

Coolant Type: None

Description: Cardboard Box

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: March 9, 2011 9:20

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Laboratory 

Page 23 of 25
Analytical Laboratory 

Page 24 of 25
Analytical Laboratory 

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