Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 1 of 37

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460

OFFICE OF PREVENTION, PESTICIDE

AND TOXIC SUBSTANCES

MEMORANDUM

Date: June 12, 2009

SUBJECT: Triticonazole. Application for New Seed Treatment Uses on Cereal Grains

(Except Rice) (PP#8F7420). Summary of Analytical Chemistry and Residue 

Data.

PC Code: 125620 DP Barcodes: 325956, 353372, and 360624

Decision No.: 359614, 394309, 399232 Registration No.: 7969-xxx

Petition No.: #8F7420 Regulatory Action: Section 3 Registration

Risk Assessment Type: NA Case No.: NA

TXR No.: NA CAS No.: 131983-72-7

MRID No.: See MRID Summary Table 40 CFR: §180.583

Ver.Apr.08

FROM: Amelia M. Acierto, Chemist

Risk Assessment Branch 3

Health Effects Division (7509P)

Through: Stephen Funk, Senior Science Advisor

Risk Assessment Branch III

 Health Effects Division (7509P)

To: Shaja Joyner, RM #20

 Fungicide Branch

 Registration Division (7505P)

MRID Summary Table

MRID No. Study Type Comments

47511902 860.1340 Crops New DER; 47511902.der.doc

45595401 860.1380 Wheat RACs and processed New DER; 45595401.der.doc

46568301 860.1380 Triazoles New DER; 46568301.der.doc

47470203 860.1500 Cereal grains New DER; 47470203.der.doc

47511901 860.1520 Wheat, corn, rice, sorghum New DER; 47511901.der.doc

47399201 860.1850 Lettuce, Radish and Wheat New DER; 47399201.der.doc
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 2 of 37

This document was originally prepared under contract by Dynamac Corporation (1901 Research 

Boulevard, Suite 220; Rockville, MD 20850). 

The document has been reviewed by the Health Effects Division (HED) and revised to reflect 

current Office of Pesticide Programs (OPP) policies.

Executive Summary

Triticonazole is a systemic triazole fungicide that is currently registered for seed treatment on 

barley and wheat for the control of various seed-borne diseases. Triticonazole is formulated as 

liquid flowable formulations. The first food/feed use was requested under PP#9F6051 in 2001 

by Aventis Crop Science (EPA Company No. 264); the Aventis products have since been 

transferred to BASF (EPA Company No. 7969).

BASF Crop Protection has submitted a tolerance petition, PP#8F7420, and Section 3 request to 

register Triticonazole HL Fungicide (EPA Reg. No. 7969-xxx, a 4.17 lb ai/gal liquid flowable

formulation) for seed treatment uses on cereal grains (except rice) at a maximum rate of 0.05 lb 

ai/100 lb seed. Concurrently, BASF has proposed the establishment of permanent tolerances for 

residues of triticonazole in/on the following crop groups:

Grain, cereal, group 15 (except rice).............................................. 0.05 ppm

Grain, cereal, forage, fodder and straw, group 16 (except rice)..... 0.10 ppm

Tolerances have been established under 40 CFR §180.583(a) for residues of the fungicide 

triticonazole, (1RS)-(E)-5-[(4-chlorophenyl)methylene]-2,2-dimethyl-1-(1H -1,2,4-triazol-1-

ylmethyl)cyclopentanol, from the treatment of seed prior to planting in/on barley grain, hay and 

straw, and wheat grain, forage, hay and straw, each at 0.05 ppm. There are no Codex or Mexican 

MRLs established for residues of triticonazole. Canadian MRLs are established for triticonazole 

in/on barley, wheat, and oats, each at 0.01 ppm. 

The nature of the residue in plant commodities is considered adequately understood only for the 

purpose of the proposed/registered seed treatment uses on cereal grain-type crops. Based on the 

barley and wheat metabolism studies, triticonazole is metabolized primarily via hydroxylation of 

the dimethylcyclopentane moiety to form a series of dihydroxy metabolites which subsequently

cleave to form numerous minor polar metabolites. No triazole metabolites [1,2,4-triazole (T), 

triazolylalanine (TA), or triazolylacetic acid (TAA)] were detected in barley or wheat grain. 

HED has previously determined that triticonazole is the residue of concern in barley and wheat 

from seed treatment uses. This determination may be extended to include seed treatment uses for 

all crop commodities in the cereal grains crop group.

The nature of the residue in livestock commodities is considered adequately understood only for 

the purpose of the proposed/registered seed treatment uses on cereal grain-type crops, based on 

marginally adequate dairy cattle and laying hen studies with phenyl-labeled triticonazole only. 

HED has previously deemed that triticonazole is the residue of concern in ruminants and poultry

from seed treatment uses on barley and wheat. This determination may again be extended to 

include seed treatment uses for all crop commodities in the cereal grains crop group. 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 3 of 37

The contribution of triazole derivatives [1,2,4-triazole, triazolylalanine, and triazolylacetic acid]

from the use of triticonazole on barley and wheat to the aggregate exposure for human health risk 

assessment has been previously considered (DP# 322215, 2/7/06, M. Doherty et al.).

An adequate LC/MS and LC/MS/MS method (Method 148.02) is available for the enforcement 

of the proposed tolerances for triticonazole in cereal grain matrices. A similar method, 

LC/MS/MS method (Method 562/0), was used for data collection in the cereal grain field trial 

and processing studies. The method was adequate for data collection based on acceptable 

method verification and concurrent method recoveries. The validated LOQ was 0.01 ppm for 

triticonazole in/on all cereal grain crop matrices.

The data requirements for testing with multiresidue methods have been fulfilled. Based on the 

results of the testing, the multiresidue methods are not appropriate for determining triticonazole

residues.

Livestock feeding studies, livestock tolerances, and livestock enforcement methods are not 

required for the purpose of this petition. Based on the recommended tolerances for triticonazole 

residues in livestock feedstuffs, the maximum reasonable dietary burdens (MRDB) for livestock 

were calculated at 0.089 ppm for beef cattle, 0.346 ppm for dairy cattle, 0.038 ppm for poultry, 

and 0.042 ppm for swine. The results of the reviewed cow and poultry metabolism studies along 

with the low levels of residues found in the cereal grain field trials indicate that there is no 

reasonable expectation that quantifiable residues of triticonazole will occur in livestock 

commodities [40 CFR §180.6(a)(3)]. HED will re-examine this determination if additional uses 

on livestock feedstuffs are proposed in the future.

Adequate data are available supporting the stability of triticonazole residues during frozen 

storage of samples collected from the cereal grain crop field trials and processing studies. 

Adequate storage stability data are also available for the triazole-related metabolites (T, TA, and 

TAA) to support the storage conditions and durations of wheat, corn, sorghum, and rice grain 

samples from the field studies, and wheat flour and polished rice from the processing study. The 

adequacy of the additional triazole storage stability data to support the storage durations of cereal 

grain forages, fodders and/or straws, corn kernel plus cob with husk removed (K+CWHR), and 

sorghum stalks from the cereal grain field trial studies will be determined when the final results 

of an ongoing triazole storage stability study are submitted to the Agency. According to current 

Agency policy, storage stability in the raw agricultural commodity (RAC) can be translated to the 

processed commodity.

The submitted magnitude of the residue data are adequate to support the requested seed treatment 

uses for cereal grain crops (except rice). There are adequate data for the representative crops of 

the cereal grain groups 15 and 16 to establish crop group tolerances. The number and locations 

of field trials were conducted according to the OPPTS 860.1500 guidelines. The seed treatment 

rate (0.05 lb ai/100 lbs seed) used in the field trials reflects the proposed use of Triticonazole HL

Fungicide (EPA Reg. No. 7969-xxx) on barley, field corn and popcorn, oats, rye, wheat, and 

triticale. However, the rate reflects 5x and 2.5x the proposed seed treatment rate for sweet corn 

and sorghum, respectively. 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 4 of 37

Residues of triticonazole were below the LOQ of 0.01 ppm in treated wheat grain, corn grain, 

corn K+CWHR, and sorghum grain. Low but quantifiable residues of triticonazole were 

observed in rice grain. The available data support a tolerance level of 0.01 ppm for cereal grains, 

group 15 (except rice). The value of 0.05 ppm proposed by the petitioner is excessive, when rice 

is excluded. Moreover, the 0.01 ppm level harmonizes with Canada. A revised Section F must 

be submitted to reflect the tolerance recommendation of 0.01 ppm for cereal grains, group 15 

(except rice). The individual tolerances established for barley grain and wheat grain at 0.05 ppm 

in 40 CFR §180.583(a) should be deleted when the crop group tolerance is established.

Residues of triticonazole were also nonquantifiable in treated field and sweet corn forage and 

stover, and sorghum forage and stover. Quantifiable residues of triticonazole were observed in 

treated wheat forage, hay and straw, and rice straw. Appropriate tolerance levels were 

determined statistically (see Appendix I) according to Guidance for Setting Pesticide Tolerances 

Based on Field Trial Data SOP. The residue data for rice straw was excluded from the 

spreadsheet because the petitioner is not applying for use on rice. The tolerance spreadsheet 

calculated tolerances of 0.20 ppm for wheat forage, 0.25 ppm for wheat hay, and 0.20 ppm for 

wheat straw. HED recommends a crop group tolerance of 0.25 ppm based on the highest 

calculated value for the representative crop (wheat hay). 

A revised Section F must be submitted to reflect the tolerance recommendation of 0.25 ppm for 

the forage, fodder, and straw of cereal grains, group 16 (except rice). The individual tolerances 

established for barley hay and straw, and wheat forage, hay and straw in 40 CFR §180.583(a) 

should be deleted when the crop group tolerance is established.

The processing data for field corn, rice, sweet sorghum, and wheat are adequate. The results 

indicate that residues of triticonazole were nonquantifiable (<0.01 ppm) in the cereal grain RACs 

(corn, rice, wheat, and sorghum) grown from seeds treated at 250 g triticonazole/100 kg seed (5x 

the maximum proposed seed treatment rate). Analysis of rice processed matrices showed that 

residues of triticonazole may concentrate in hulls (2.8x) but not significantly in bran (1.1x); 

residues in polished rice were nonquantifiable. As the petitioner is not seeking to register use on 

rice at this time, no tolerances are required for rice processed matrices.

The confined rotational crop study is acceptable. The application rate used in the study reflects 

1.4x the equivalent per acre rate for cereal grain crops. The metabolism of triticonazole in 

rotated crops appears similar to those observed for primary crops, except that triazole-related 

metabolites were detected in the rotated crops. The data indicate that residues of triticonazole 

may be present at >0.01 ppm in 30-DAT rotated lettuce (0.017 ppm), radish tops (0.049 ppm) 

and wheat forage (0.041 ppm), straw (0.133 ppm) and chaff (0.139 ppm); 120-DAT rotated 

radish tops (0.016 ppm), and wheat forage (0.019 ppm), straw (0.150 ppm), and chaff (0.114 

ppm); and 365-DAT rotated wheat forage (0.025 ppm), straw (0.086 ppm) and chaff (0.133 

ppm). Wheat hay was not analyzed but forage and straw were analyzed, and residues of 

triticonazole were found in forage and straw.

The proposed label for the 4.17 lb/gal FS specifies that “planting of any crop not listed on the 

label within 30 days after planting treated seed is not allowed”. The results of the submitted 

confined rotational crop study do not support this restriction. The data from the confined 

rotational crop study indicate that triticonazole residues greater than 0.01 ppm were detected at 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 5 of 37

the 30-day PBI. To support a 30-day plantback restriction on the label, limited field rotational 

crop data for leafy vegetables, root and tuber vegetables, foliage of root and tuber vegetables, and 

a cereal grain are required. Until the requested data are provided, the label must be modified to 

specify a 120-day (4-month) plantback restriction for leafy vegetables, a 365-day (12-month) 

plantback restriction for root and tuber vegetables, and prohibit the planting of all other crops not 

listed on the label.

Analytical standards for triticonazole are currently available in the EPA National Pesticide 

Standards Repository.

Regulatory Recommendations and Residue Chemistry Deficiencies

HED has examined the residue chemistry database for triticonazole and concludes that pending 

submission of a revised Section B (see requirements under Directions for Use) and a revised 

Section F (see requirements under Proposed Tolerances), there are no residue chemistry issues 

that would preclude granting conditional registration for the requested seed treatment uses on 

cereal grain crops (except rice) and the establishment of tolerances for residues of the fungicide 

triticonazole, (1RS)-(E)-5-[(4-chlorophenyl)methylene]-2,2-dimethyl-1-(1H -1,2,4-triazol-1-

ylmethyl)cyclopentanol, from the treatment of seed prior to planting in/on: 

Grain, cereal, group 15 (except rice).......................................0.01 ppm

Grain, cereal, forage, fodder, and straw, group 16 (except rice) ......0.25 ppm

Moreover, upon establishment of the above tolerances for grain, cereal, group 15 (except rice) 

and grain, cereal, forage, fodder, and straw, group 16 (except rice), the existing tolerances listed 

in 40 CFR 180 (a) should be removed: barley, grain; barley, hay; barley, straw; wheat, forage; 

wheat, grain; wheat, hay; wheat, straw.

A human health risk assessment is forthcoming.

860.1200 Directions for Use

• The label for Triticonazole HL Fungicide must be amended to include the following 

rotational crop restrictions: a 120-day plantback interval for leafy vegetables, a 365-

day plantback interval root and tuber vegetables, and prohibit the planting of all other 

crops not listed on the label.

860.1550 Proposed Tolerances

• A revised Section F must be submitted to reflect the tolerance recommendation of 0.01 

ppm for cereal grains, group 15 (except rice); to reflect the tolerance recommendation 

of 0.25 ppm for grain, cereal, forage, fodder, and straw, group 16 (except rice); and to 

reflect the correct commodity definitions as listed in Table 9.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 6 of 37

HED recommends conversion of conditional registration to unconditional registration for 

the cereal grains (except rice) upon adequate resolution of data pertaining to storage 

stability and field accumulation in rotational crops.

860.1380 Storage Stability

• To support the reported results for 1,2,4-triazole and the triazole conjugates, the final 

report of the ongoing storage stability study with free triazole and triazole conjugates in 

plant commodities must be submitted.

860.1900 Field Accumulation in Rotational Crops

• Data from limited field accumulation studies on rotational crops are required.

Background

The chemical structure and nomenclature of triticonazole and triazole-related metabolites, and 

the physicochemical properties of the technical grade of triticonazole are, respectively, presented 

in Tables 1 and 2.

Table 1. Triticonazole Nomenclature.

Chemical structure

H C3

H3C

OH

N

N

N

Cl

Common name Triticonazole

Company experimental name BAS 595 F (formerly RPA 4000727)

IUPAC name (±)(E)-5-(4-chlorobenzylidene)-2,2-dimethyl-1-(1H-1,2,4-triazol-1-

ylmethyl)cyclopentanol

CAS name 5-[(4-chlorophenyl)methylene]-2,2-dimethyl-1-(1H-1,2,4-triazol-1-

ylmethyl)cyclopentanol

CAS registry number 131983-72-7

End-use product (EP) Triticonazole HL (4.17 lb ai/gal flowable liquid; EPA Reg. No. 7969-xxx)

Chemical structure of T

metabolite

HN

N

N

1,2,4-Triazole

Chemical structure of TA

metabolite

N

N

N

HO

NH2

O

Triazole alanine or triazolylalanine
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 7 of 37

Table 1. Triticonazole Nomenclature.

Chemical structure of TAA

metabolite

N

N

N

HO

O

Triazole acetic acid or triazolylacetic acid

Table 2. Physicochemical Properties of Chemical: Triticonazole.

Parameter Value Reference

Melting point/range 140 oC; PAI displays two crystalline forms 

melting at 137 and 141 oC

BASF document number 

2002/1016091 1

pH Not available

Density 1.21 g/mL (PAI) BASF document number 

2002/1016091 1

Water solubility 8.4 mg/L at 20 oC

Solvent solubility (at 20 oC) Hexane 0.12 g/L

Methanol 18.2 g/L

Acetone 74.5 g/L

Toluene 12.6 g/L

Dichloromethane 191 g/L

Ethyl acetate 48.6 g/L

n-Octanol 6.2 g/L

Vapor pressure <0.1 x 10-5PA at 50 oC

Dissociation constant, pKa Does not dissociate

Octanol/water partition coefficient, 

Log(KOW)

Log P = 3.29 at 20 oC

UV/visible absorption spectrum Not available

1 As referenced in MRID 47470203.

860.1200 Directions for Use

A summary of the proposed use directions is presented in Table 3. Information pertaining to the 

proposed use pattern was obtained from a 2008 specimen label submitted for Triticonazole HL

Fungicide Seed Treatment (4.17 lb/gal FS). The EP is proposed for the control of various seedborne diseases in cereal crops except rice. 

Table 3 shows that Triticonazole HL Fungicide Seed Treatment is proposed for seed treatment on 

cereal grains (except rice) at a maximum rate of 0.05 lb ai/100 lb seed. The end-use product 

Charter® Fungicide Treatment (EPA Reg. No. 7969-386; accepted 6/27/2007) is currently

registered on barley and wheat for preplant seed treatment at a maximum rate of 0.005 lb ai/100 

lb seed.

Table 3. Summary of Proposed Seed Treatment Use of Triticonazole.

Application 

Type

Formulation

[EPA Reg. No.]

Seed Treatment 

Application Rate

(lb ai/100 lb seed)

Maximum 

Seeding Rate 1

(lb seed planted/A)

Maximum Equivalent 

Field Application Rate 2

(lb ai/A)

Barley

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.005-0.050 96 0.048

Field Corn and Popcorn (includes seed production)
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 8 of 37

Table 3. Summary of Proposed Seed Treatment Use of Triticonazole.

Application 

Type

Formulation

[EPA Reg. No.]

Seed Treatment 

Application Rate

(lb ai/100 lb seed)

Maximum 

Seeding Rate 1

(lb seed planted/A)

Maximum Equivalent 

Field Application Rate 2

(lb ai/A)

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.005-0.050 15 0.008

Sweet Corn

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.010 15 0.002

Oats

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.005-0.050 128 0.064

Rye

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.005-0.050 112 0.056

Sorghum

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.010-0.020 10 0.002

Wheat and Triticale

Seed 

Treatment

4.17 lb/gal FS

[7969-xxx] 0.005-0.050 150 0.075

General Use Directions and Limitations: To be applied as a water-based mixture using standard or mist-type 

seed treatment application equipment. A colorant must be added to identify the treated seed. The proposed label 

does not include water amounts needed to provide the mixture or slurry rate for optimum coverage, but 

recommends consulting a seed-treatment specialist. The product may be tank-mixed with other water-based seed 

treatments. Use of treated seed for food, feed, or oil processing is prohibited. Planting of any crop not listed on 

the label within 30 days after planting treated seed is not allowed. 

1

 From Science Advisory Council for Exposure, Standard Operating Procedure, SOP #15, 3/2/04.

2

 Calculated by the study reviewer using the maximum seed treatment rate and maximum seeding rate.

Conclusions. The submitted label for Triticonazole HL Fungicide Seed Treatment is adequate to 

allow evaluation of the residue data relative to the proposed use. However, the proposed 30-day

plantback interval for all crops not listed on the label is not acceptable. The submitted confined 

rotational crop study, which was conducted at 1.4x the proposed maximum equivalent field 

application rate for cereal grains, indicates that residues of triticonazole may be present at >0.01 

ppm in 30-DAT rotated lettuce, radish tops and wheat forage, straw and chaff; 120-DAT rotated 

radish tops, and wheat forage, straw, and chaff; and 365-DAT rotated wheat forage, straw and 

chaff.

The label for Triticonazole HL Fungicide must be amended to include the following rotational 

crop restrictions: a 120-day plantback interval for leafy vegetables, a 365-day plantback interval 

for root and tuber vegetables, and prohibit the planting of all other crops not listed on the label. 

Data from limited field accumulation studies on rotational crops are required.

860.1300 Nature of the Residue - Plants

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 9 of 37

Field and greenhouse studies were previously submitted investigating the metabolism of 

[triazole14C]- and [phenyl14C]-triticonazole in spring and winter wheat and barley. In the 

initial greenhouse study and two field studies (wheat and barley seeds treated with [triazole14C]triticonazole), the total radioactive residues (TRRs) were determined, but samples were 

either not extracted or the characterization of extracted 14C-residues was very limited. The most 

complete analyses were conducted in two field studies: (i) with [triazole14C]triticonazole at 187 

g ai/100 kg seed (3.7x the maximum proposed seed treatment use of 50 g ai/100 kg seed) in 

wheat and 299 g ai/100 kg seed (6x) in barley; and (ii) with [phenyl14C]triticonazole at 155 g 

ai/100 kg seed (3.1x) in wheat and 242 g ai/100 kg seed (4.8x) in barley. In these studies, the 

14C-residues in RAC samples were identified as parent, one of the dihydroxy metabolites (RPA 

404766, RPA 404886, RPA 406341, RPA 406780), the Z isomer of parent (RPA 406203), or 

characterized as minor unknowns (each <10% TRR) that were generally polar in nature. 

However, extraction of various samples in these studies was incomplete as residual solids 

accounted for >10% of the TRR and >0.05 ppm.

As determined in the [triazole14C]-triticonazole metabolism study, no triazole, triazolylalanine, 

or triazolylacetic acid were detected in wheat and barley grain. These are metabolites of some 

triazole fungicides, for which free triazole may be of toxicological concern. The petitioner has 

been advised that in future plant metabolism studies, which may be conducted to reflect future 

application methods (i.e., foliar or soil application), the petitioner should also look for triazole 

pyruvate.

The metabolism of triticonazole in wheat and barley was considered adequately understood for 

the purposes of the use of triticonazole as a seed treatment on cereal grains. Based on the 

available wheat and barley metabolism data, triticonazole is metabolized in wheat and barley

primarily via hydroxylation of the dimethylcyclopentane moiety to form a series of dihydroxy

metabolites. The residues which were identified were parent, RPA 404766, RPA 404886, RPA 

406341, RPA 406780, and the Z isomer of the parent (RPA 406203). Subsequent cleavage of the 

dihydroxy metabolites results in the formation of numerous minor (<10% TRR) metabolites that 

are generally more polar in nature. HED’s Metabolism Assessment Review Committee 

determined on 5/1/01 that triticonazole per se is the residue of concern in wheat and barley for 

seed treatment uses. The available metabolism studies supporting seed treatment uses on barley

and wheat may be extended to include seed treatment uses on all crop commodities in the cereal 

grains crop group. 

860.1300 Nature of the Residue - Livestock

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

Dairy cow and hen studies were previously submitted. One ruminant metabolism study was 

conducted on dairy cows using [U-phenyl14C]-triticonazole at dosing levels of 1 ppm (3.1x the 

MRBD for dairy cattle) and 10 ppm (31x) for seven consecutive days. Detectable radioactive 

residues were found in liver, high-dose kidney, and high-dose milk. Residues identified by

HPLC in the liver were parent, RPA 404886, RPA 406341, RPA 406780, RPA 404766, and/or

RPA 406972 (each <0.001 ppm in low dose). Residues identified by HPLC in the high-dose 

kidney were parent, RPA 404886, RPA 406341, RPA 404766, and RPA 406972. The identity of 

RPA 406780 in high-dose liver was confirmed by MS. RPA 406780 was identified as the major 

residue in high-dose liver. The identity of RPA 404886 in high-dose kidney was confirmed by
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 10 of 37

TLC. Residues in milk, muscle, and fat were not identified since radioactive residues were 

nondetectable (<0.001 ppm in milk and <0.004 ppm in muscle and fat) at the 1 ppm dosing level, 

and ≤0.001 ppm in milk and nondetectable (<0.004 ppm) in muscle and fat at the 10 ppm dosing 

level.

A poultry metabolism study was conducted on laying hens using [U-phenyl14C]-triticonazole at 

dosing levels of ~1 ppm (125x the MRBD for poultry) and ~10 ppm (1250x) for 14 consecutive 

days. Detectable radioactive residues were found in whole eggs, liver, high-dose muscle, highdose skin with fat, and high-dose peritoneal fat. Residues identified by HPLC in high-dose eggs

were parent, RPA 404886, RPA 404766, RPA 406780, RPA 406972, and RPA 406341. 

Residues identified by HPLC in high-dose liver were parent, RPA 404886, and RPA 406972. 

Residues identified in peritoneal fat by TLC subsequent to HPLC were parent, RPA 406341, and 

RPA 404886. Residues in poultry muscle were not identified because of low radioactivity. 

The metabolism of triticonazole in ruminants and poultry is considered adequately understood

for the purposes of uses of triticonazole as a seed treatment on cereal grains. The HED 

Metabolism Assessment Review Committee determined on 5/1/01 that triticonazole is the 

residue of concern in livestock for seed treatment uses on barley and wheat. The available 

metabolism studies supporting seed treatment uses on barley and wheat may be extended to 

include seed treatment uses on all crop commodities in the cereal grains crop group. 

For future uses other than seed treatment uses, ruminant and poultry metabolism studies with the 

14C label in the triazole ring will be needed in addition to new phenyl-labeled ruminant and 

poultry metabolism studies required because of multiple deficiencies regarding the submitted 

phenyl-labeled studies.

860.1340 Residue Analytical Methods

Enforcement method

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

In conjunction with a previous triticonazole petition (PP#9F6051) for seed treatment uses on 

barley and wheat, HED has determined that the LC/MS and LC/MS/MS methods (Aventis 

Method MS 148.02) are suitable for enforcing tolerances for residues of triticonazole and two of 

its dihydroxy metabolites RPA 404886 and RPA 406341. The method measures each analyte 

individually in/on wheat and barley RACs and processed commodities. Using this method, 

residues are extracted with water:acetone. No hydrolysis step is included to release conjugate 

residues. The reported LOQ for the LC/MS/MS method was 0.002 ppm for all three analytes in 

forage, straw and grain. A successful independent laboratory validation has been conducted for 

Aventis Method MS 148.02 on wheat forage. 

HED concludes that Aventis Method MS 148.02 would also be adequate for the enforcement of 

recommended tolerances addressed in this document. For future uses other than seed treatment, 

additional data regarding the analytical method must be addressed (see DP# 262566, 6/6/2001, 

N. Dodd).
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 11 of 37

Data-Collection Method

DER Reference: 47511902.der.doc

Samples of RACs (wheat forage, hay, straw and grain; field corn, forage, grain and stover; sweet 

corn forage, K+CWHR, and stover; sorghum forage, grain, and stover; and rice grain and straw) 

and processed (wheat bran and flour; corn oil and starch; and rice hulls, bran and polished rice)

commodities were analyzed for residues of triticonazole using LC/MS/MS Method 562/0. The 

data-collection method is similar to the enforcement method discussed above. Method 562/0 

method was modified for analysis of cereal grain matrices to eliminate the clean-up step which 

was not needed because no matrix interference occurred.

 

BASF has submitted descriptions and validation data (MRID 47511902) for Method 562/0. 

Using this method, residues in crop samples are extracted with a mixture of methanol and water, 

centrifuged, and then partitioned with dichloromethane. Triticonazole residues are quantitated by

LC/MS/MS. Acceptable method validation recoveries were obtained for triticonazole from bean, 

corn grain, straw and forage, lemon, lettuce, and rapeseed fortified with triticonazole at 0.01 and 

0.10 ppm. Residues of triticonazole were quantitated using both mass ion transitions 

(318.0→70.0 and 318.0→125.0). Average recoveries were within the acceptable range of 70-

120% and RSDs were <20% for triticonazole in all tested matrices. The fortification levels used 

in method validation are adequate to bracket expected residue levels in cereal grain matrices 

from the associated field trials. Confirmatory analysis may be performed using the second 

daughter ion transition.

Cereal grain RACs and processed samples were also analyzed for the triazole-related 

metabolites, triazole (T), triazolylacetic acid (TAA) and triazolylalanine (TA) using LC/MS/MS 

Methods D0508 and D0604 (grain). Briefly, residues in samples were extracted with acetonitrile 

(ACN):water (70:30, v:v). The extract was evaporated to dryness (or near dryness for grain and 

K+CWHR), redissolved in 0.1% formic acid in water and filtered through a syringe filter for 

LC/MS/MS analysis. Corn oil samples were extracted with ACN/0.1% formic acid in water and 

hexane using liquid-liquid partitioning. The hexane phase was discarded, and the remainder 

evaporated to dryness and redissolved in 0.1% formic acid in water for analysis. MS/MS 

detection in the positive ionization mode was used to monitor ion transitions of m/z 70.1 → 43.2 

for T, m/z 128.1 → 70.2 for TAA, and m/z 157.1 → 88.1 for TA. In the field trials, the validated 

LOQ was 0.05 ppm each for T, TA, and TAA in all cereal grain matrices, except for T and TAA 

in grain and K+CWHR, which had LOQs of 0.01 ppm each. In the processing studies, the 

validated LOQ was 0.01 ppm each for T and TAA, and 0.05 ppm for TA in cereal grain matrices.

The above LC/MS/MS methods (562/0 for triticonazole; D0508 and D0604 for triazoles) were 

adequately validated in conjunction with the analysis of field trial and processing samples using 

untreated samples of each commodity fortified with the appropriate analytes at levels bracketing 

the residues found in the treated samples. The petitioner reported that because triazole-related 

compounds are commonly found in plants in nature, two untreated controls were used to 

establish the background apparent residues in controls for each analytical set.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 12 of 37

Samples from a wheat storage stability study were analyzed for residues of triticonazole and its 

metabolites RPA 406341 and RPA 404886 using the enforcement method LC/MS Method 

148.02 (revision 2) entitled “Method of Analysis for the Determination of RPA 400727 

(Triticonazole) and its Metabolites RPA 404886 and RPA 406341 in Wheat and Barley Matrices 

by LC/MS.” The validated LOQ based on method verification data was 0.05 ppm for each 

analyte in all wheat matrices. Briefly, forage, hay, straw, germ, and bran samples were soaked 

overnight in acetone, and extracted twice again with water:acetone (1:9, v:v). The concentrated 

extract was mixed with ACN and diluted with water for clean-up through a C18 solid phase 

extraction (SPE) cartridge; residues were eluted with 5% methanol in ACN. The eluate was 

evaporated to just dryness and reconstituted in ACN:water (1:1, v:v) for LC/MS analysis.

Grain and flour samples were soaked overnight in water:acetone (9:1, v:v) and extracted twice 

again with acetone. The concentrated extract was mixed with a small amount of acetone, and 

water with sodium chloride for partitioning with DCM. Partitioning with DCM was repeated 

twice. The DCM phases were combined, evaporated to just dryness and reconstituted in 

ACN:water (1:1, v:v) for LC/MS analysis.

The method was adequately validated prior to and in conjunction with the analysis of the stored 

samples. Concurrent recoveries were generally within the acceptable range of 70-120% for 

triticonazole and the two metabolites in all wheat commodities fortified with each analyte at 0.50 

ppm. Acceptable method verification recoveries were also obtained for triticonazole residues in 

wheat matrices fortified at 0.05 and 0.50 ppm.

Conclusions. The LC/MS/MS method, 562/0, is adequate for collecting data on residues of 

triticonazole in/on cereal grain RACs (wheat forage, hay, straw and grain; field corn, forage, 

grain and stover; sweet corn forage, K+CWHR, and stover; sorghum forage, grain, and stover; 

and rice grain and straw) and processed (wheat bran and flour; corn oil and starch; and rice hulls, 

bran and polished rice) commodities. The LC/MS/MS methods, Methods D0508 and D0604, are

adequate for collecting data on residues of 1,2,4-triazole, TA and TAA in/on cereal grain

matrices.

The previously submitted LC/MS and LC/MS/MS methods (Aventis Method MS 148.02) would 

also be suitable for enforcing the recommended tolerances. As noted under PP#9F6051, for 

future uses other than seed treatment, additional data regarding the analytical method must be 

addressed. 

No methods have been submitted or are required for analysis of triticonazole residues in 

livestock commodities. The proposed seed treatment use on cereal grain crops falls under 40 

CFR §180.6(a)(3).

860.1360 Multiresidue Methods

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

Multiresidue method testing data were previously submitted for triticonazole, RPA 406341, and 

RPA 404886. The multiresidue methods are not adequate for enforcement since recoveries were 

obtained only with Protocol D, and the recoveries were not adequate. These data were forwarded 

to FDA.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 13 of 37

860.1380 Storage Stability

Storage Stability Study with Triticonazole on Wheat

DER Reference: 45595401.der.doc

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

BASF has submitted the final report of a storage stability study with triticonazole on wheat. 

Interim data up to 12 months were previously reviewed in connection with a tolerance petition 

for wheat and barley (PP#9F6051; DP# 262566, 6/6/2001, N. Dodd). The final study report 

reflects a maximum storage interval of 18 months and was required as a condition of registration 

under PP#9F6051. Samples of untreated wheat grain, forage, straw, hay and processed bran, 

flour and germ were fortified with triticonazole or its metabolites RPA 406341 and RPA 404886 

at 0.5 ppm each analyte, except for months 1 through 18 where RPA 404886 was fortified at 0.28 

ppm in all matrices. Samples were stored frozen (-20 oC) for up to 18 months. Actual storage 

intervals tested were 0, 1, 3, 6, 9, 12, 15, and 18 months.

Wheat samples were analyzed for residues of triticonazole and its metabolites RPA 406341 and 

RPA 404886 using LC/MS Method 148.02 (revision 2), entitled “Method of Analysis for the 

Determination of RPA 400727 (Triticonazole) and its Metabolites RPA 404886 and RPA 406341 

in Wheat and Barley Matrices by LC/MS.” Samples were analyzed by Enviro-Test Laboratories 

(Edmonton, Alberta, Canada). The method was adequate for data collection based on acceptable 

concurrent method recoveries; the validated LOQ based on method verification data was 0.05 

ppm for each analyte in all wheat matrices.

The final study results indicate that residues of triticonazole and its metabolites RPA 406341 and 

RPA 404886 were relatively stable in/on the raw agricultural commodities of wheat (forage, hay, 

grain, and straw) as well as in its processed commodities (germ, flour, and bran) when stored 

frozen (-20 oC) for up to 18 months.

Interim Results of a Storage Stability Data Study with 1,2,4-Triazole (T), Triazolylalanine (TA), 

and Triazolylacetic Acid (TAA) In Apples, Wheat Flour, Peanut Butter, Milk, and Eggs

DER Reference: 46568301.der.doc

The U.S. Triazole Task Force has submitted the interim results of an ongoing storage stability

study with 1,2,4-triazole (T), triazolylalanine (TA) and triazolylacetic acid (TAA) in selected 

crop and animal matrices (apples, wheat flour, peanut butter, milk, and eggs). The study

submission reported that when completed, the final study report will include analysis of 

additional samples for up to a two-year time point. Homogenized samples of apples, eggs, and 

milk were spiked with T at 0.20 ppm, or a mixture of TA and TAA at 0.20 ppm for each analyte. 

Samples of homogenized wheat flour and peanut butter were spiked with T at 0.20 ppm, or TA 

and TAA at 1.0 ppm for each analyte. The fortification level used for peanut butter cannot be 

confirmed; therefore, raw data are required in the final study report to confirm the actual 

fortification levels. Fortified and unfortified samples were stored frozen (≤-20 oC) and analyzed 

following 0, 1, 3, 6, and 12 months of storage.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 14 of 37

Samples were analyzed for residues of T, TA, and TAA using LC/MS/MS Method Meth-160 

(Pyxant Labs Inc. Method Addendum Revision #2). Adequate concurrent method recovery data 

were provided to confirm that LC/MS/MS Method Meth-160 is adequate for data collection. The 

validated LOQs were 0.010 ppm for each analyte in apples; 0.010 ppm each for TA and TAA, 

0.005 ppm for T in milk; and 0.010 ppm for T and 0.030 ppm each for TA and TAA in wheat 

flour. 

The interim data suggest that residues of T, TA and TAA are reasonably stable when stored 

frozen for up to one year in/on apples, wheat flour, peanut butter, milk, and eggs. The Task 

Force noted that the protocol specified that peanut butter be stored refrigerated (<10 oC) for the 

storage stability study to support the USTTF and USDA PDP (Pesticide Data Program) 

monitoring studies. In a deviation from the protocol, peanut butter samples were instead stored 

frozen (<-20 oC). The peanut butter storage stability testing is planned to be repeated with 

samples stored refrigerated.

Other Storage Stability Data

Interim storage stability data for T, TA, and TAA in canola seed, turnip roots, mustard greens, 

and wheat straw, grain, and forage were previously referenced in association with a 

prothioconazole petition (PP#4F6830; DP#s 303508 & 314517, S. Funk, 8/21/2006). The final 

report on the storage stability study with the triazoles which are required as confirmatory data;

that may support the storage conditions and durations of wheat, corn, sorghum, and rice foliage 

matrices and corn K+CWHR have been received and is being reviewed separately.

Sample storage conditions and intervals

Table 4 lists the information pertaining to the storage conditions and intervals of samples 

incurred in the current field trial and processing studies.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 15 of 37

Table 4. Summary of Storage Conditions and Durations of Samples from Crop Field Trial and 

Processing Studies. 

Matrix Storage

Temp. (°C)

Actual Storage

Duration

Interval of Demonstrated 

Storage Stability

Triticonazole

Wheat forage <-5 224-323 days (7.4-10.6 months) Residues of triticonazole are relatively stable in/on 

wheat forage, hay, straw and grain stored frozen for 

up to 18 months.

Wheat hay 200-295 days (6.6-9.7 months)

Wheat grain 175-261 days (5.8-8.6 months)

Wheat straw 173-265 days (5.7-8.7 months)

Corn forage 124-256 days (4.1-8.4 months)

Corn grain 106-184 days (3.5-6.1 months)

Corn stover 109-342 days (3.6-11.3 months)

Corn K+CWHR 105-258 days (3.5-8.5 months)

Sorghum forage 142-247 days (4.7-8.1 months)

Sorghum grain 109-158 days (3.6-5.2 months)

Sorghum stover 112-161 days (3.7-5.3 months)

Rice grain 97-226 days (3.2-7.4 months)

Rice straw 106-182 days (3.5-6.0 months)

Corn, grain

(for processing)

<-5 120 days (3.9 months) 18 months in wheat grain

Rice, grain

(for processing)

131-203 days (4.3-6.7 months) 18 months in wheat grain

Rice, polished 131-203 days (4.3-6.7 months) 18 months in wheat grain

Rice, hulls 30 days (1.0 month) None required

Rice, bran 30-47 days (1.0-1.5 months) 18 months in wheat bran

Sorghum, stalk

(for processing)

154 days (5.1 months) 18 months in wheat grain and forage

Wheat, grain

(for processing)

173 days (5.7 months) 18 months in wheat grain

Triazoles

Wheat grain <-5 185-309 days (6.1-10.2 months) Residues of T, TA, and TAA are relatively stable in 

wheat flour (and apple, peanut butter, milk and 

eggs) stored frozen for up to 12 months).

Corn grain 114-192 days (3.8-6.3 months)

Sorghum grain 107-155 days (3.5-5.1 months)

Rice grain 102-177 days (3.4-5.8 months)

Corn K+CWHR 96-249 days (3.2-8.2 months) Awaiting final report

Wheat forage 229-323 days (7.5-10.6 months)

Wheat hay 217-310 days (7.1-10.2 months)

Wheat straw 182-267 days (6.0-8.8 months)

Corn forage 119-250 days (3.9-8.2 months)

Corn stover 99-332 days (3.3-10.9 months)

Sorghum forage 145-250 days (4.8-8.2 months)

Sorghum stover 119-167 days (3.9-5.5 months)

Rice straw 107-183 days (3.5-6.0 months)
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 16 of 37

Table 4. Summary of Storage Conditions and Durations of Samples from Crop Field Trial and 

Processing Studies. 

Matrix Storage

Temp. (°C)

Actual Storage

Duration

Interval of Demonstrated 

Storage Stability

Corn, grain

(for processing)

<-5 142-206 days (4.7-6.8 months) 12 months in wheat flour

Corn, grits 70-71 days (2.3 months) None available*

Corn, flour 70-71 days (2.3 months) 12 months in wheat flour

Corn, oil 11-12 days (0.4 months) None required

Corn, meal 70-71 days (2.3 months) None available*

Corn, starch 8-9 days (0.3 months) None required

Rice, grain

(for processing)

<-5 154-210 days (5.1-6.9 months) 12 months in wheat flour

Rice, polished 28-37 days (0.9-1.2 months) 12 months in wheat flour

Rice, hulls 28-40 days (0.9-1.3 months) None available*

Rice, bran 28-31 days (0.9-1.0 months) None required

Sorghum, stalk

(for processing)

177-178 days (5.8-5.9 months) Awaiting final report

Sorghum, syrup 21 days (0.7 months) None required

Wheat, grain 292-315 days (9.6-10.4 months) 12 months in wheat flour

Wheat, bran 54-62 days (1.8-2.0 months) None available*

Wheat, flour 53-57 days (1.7-1.9 months) 12 months in wheat flour

Wheat, middlings 55-56 days (1.8 months) None available*

Wheat, shorts 55-56 days (1.8 months) None available*

Wheat, germ 55-68 days (1.8-2.2 months) None available*

*According to current HED policy, storage stability in the raw agricultural commodity can be translated to the processed 

commodity.

Conclusions. The available storage stability data for triticonazole residues in wheat matrices are 

adequate and may be translated to the other cereal grains (corn, sorghum, and rice) to support the 

storage durations and conditions of samples from the submitted field and processing studies.

The interim storage stability data submitted by the U.S. Triazole Task Force for the triazolerelated metabolites suggest that residues of T, TA and TAA are reasonably stable when stored 

frozen for up to one year in/on apples, wheat flour, peanut butter, milk, and eggs. The data for 

wheat flour may be translated to wheat, corn, sorghum, and rice grain samples from the field and 

processing studies, and to corn and wheat flour and polished rice from the processing study. 

The adequacy of the additional triazole storage stability data to support the storage durations of 

cereal grain forages, fodders and/or straws, corn K+CWHR, and sorghum stalks from the cereal 

grain field trial will be determined when the final results of the ongoing triazole storage stability

study are submitted to the Agency. Supporting storage stability data for triazole residues are not 

required for corn oil and starch; rice bran; and sorghum syrup as these matrices were analyzed 

within 30 days of processing. However, no additional supporting freezer storage stability data 

are required for T, TA, and TAA in rice hulls, corn grits and meal, and wheat bran, middlings, 

shorts, and germ since, according to current Agency policy, storage stability in the raw 

agricultural commodity (RAC) can be translated to the processed commodity.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 17 of 37

860.1400 Water, Fish, and Irrigated Crops

There are no proposed uses relevant to this guideline topic. The petitioner noted that they are not 

seeking to register use on rice, a crop which may be irrigated, at this time.

860.1460 Food Handling

There are no proposed uses that are relevant to this guideline topic. 

860.1480 Meat, Milk, Poultry, and Eggs

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

The maximum theoretical dietary burdens (MTDB) for livestock were previously calculated as 

0.09 ppm for beef cattle, 0.14 ppm for dairy cattle, and 0.05 ppm for poultry and swine, based on 

barley and wheat seed treatment uses (PP#9F6051). No tolerances were established for residues 

of triticonazole in livestock commodities.

Based upon the proposed seed treatment uses on additional cereal grain crops and the 

recommended tolerances for triticonazole residues in/on livestock feedstuffs, the maximum 

reasonable dietary burdens (MRDB) for livestock are 0.089 ppm for beef cattle, 0.346 ppm for 

dairy cattle, 0.038 ppm for poultry, and 0.042 ppm for swine (see Table 5 below).

Table 5. Calculation of Dietary Burdens of Triticonazole Residues to Livestock.

Feedstuff Type1 % Dry 

Matter2 % Diet2 Recommended Tolerance (ppm)

Dietary Contribution 

(ppm)

3

Beef Cattle

Wheat, hay R 88 15 0.25 0.043

Sorghum, grain CC 86 40 0.05 0.023

Wheat milled bypdts CC 88 40 0.05 0.023

Untreated PC PC -- 5 -- 0

TOTAL BURDEN -- -- 100 -- 0.089

Dairy Cattle

Oat, forage R 30 30 0.20 0.200

Wheat, forage R 25 15 0.20 0.120

Sorghum, grain CC 86 45 0.05 0.026

Untreated PC PC -- 10 -- 0

TOTAL BURDEN -- -- 100 -- 0.346

Poultry

Wheat, grain CC 89 75 0.05 0.038

Untreated PC PC -- 25 -- 0

TOTAL BURDEN -- -- 100 -- 0.038

Swine

Corn, field, grain CC 88 85 0.05 0.042

Untreated PC PC -- 15 -- 0

TOTAL BURDEN -- -- 100 -- 0.042

1

 R: Roughage; CC: Carbohydrate concentrate; PC: Protein concentrate.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 18 of 37

2

 OPPTS 860.1000 Table 1 Feedstuffs (June 2008). 

3

 Contribution = ([tolerance /% DM] X % diet) for beef and dairy cattle; contribution = ([tolerance] X % diet) for 

poultry and swine.

Ruminant and poultry feeding studies have not been submitted, and none are required at this 

time. Based on the available cow and poultry metabolism studies, and low levels of residues 

found in the cereal grain (corn, rice, sorghum and wheat) field trials, there is no reasonable 

expectation that quantifiable residues of triticonazole will occur in livestock commodities [40 

CFR §180.6(a)(3)]. Therefore, livestock feeding studies and tolerances are not required at this 

time. If the use of triticonazole is expanded to include uses other than seed treatments or 

additional livestock feed items, the need for feeding studies will be re-evaluated. 

860.1500 Crop Field Trials

DER Reference: 47470203.der.doc

HED had previously reviewed magnitude of the residue data (PP#9F6051; DP# 262566, 

6/6/2001, N. Dodd) for triticonazole and its metabolites RPA 406341 and RPA 404886 in/on

barley and wheat following preplant seed treatment. BASF Crop Protection is now seeking the 

registration of Triticonazole HL Fungicide (EPA Reg. No. 7969-xxx) for preplant seed treatment 

uses on cereal grain crops (except rice). The supporting magnitude of the residue study (MRID 

47470203) has been reviewed, and the Executive Summary of the Data Evaluation Record is 

reproduced below.

BASF has submitted field trial data for triticonazole on corn (field and sweet), rice, sorghum, and

wheat, the representative crops of the cereal grain crop groups 15 and 16. A total of 58 field 

trials was conducted in the United States and Canada during the 2006-2007 growing season. 

Fifteen wheat trials were conducted in Zones 2 (GA; 1 trial), 4 (AR; 1 trial), 5 (KS, ND, MN; 3 

trials), 6 (TX; 1 trial), 7 (ND, NE, SD; 4 trials), 8 (OK, TX; 4 trials), and 11 (WA; 1 trial). 

Fifteen field corn trials were conducted in Zones 1 (PA; 1 trial), 2 (GA; 1 trial), 5 (IA, IL, IN, 

KS, MB, MN, ND, SD, WI; 12 trials), and 6 (TX; 1 trial). Four sweet corn trials were conducted 

in Zones 3 (FL; 1 trial), 10 (CA; 1 trial), 11 (ID, 1 trial), and 12 (BC; 1 trial). Twelve grain 

sorghum trials were conducted in Zones 2 (GA; 1 trial), 4 (AR; 1 trial), 5 (IA, IL, KS and MN; 4 

trials), 6 (OK, TX; 2 trials), 7 (NE; 1 trial), and 8 (TX; 3 trials). Finally, twelve rice trials were 

conducted in Zones 4 (AR, MS, LA; 7 trials), 5 (MO; 1 trial), 6 (TX; 2 trials), and 10 (CA; 2 

trials). 

At each test location, the crops were grown from seeds treated with a 4.2 lb/gal flowable liquid 

formulation of triticonazole, prior to planting, at 50 g ai/100 kg seed (0.05 lb ai/100 lb seed; 1x 

the maximum proposed seed treatment use on cereal grain crops). The triticonazole formulation 

was mixed with Apron XL (mefenoxam; a seed fungicide) and colorant. A separate plot 

(untreated) was planted with seed treated with Apron XL and colorant only. The applications 

made to sorghum seed also included pyraclostrobin, and sorghum plants were also foliarly treated 

with pyraclostrobin; these pyraclostrobin data are not reviewed herein. 

The cereal RAC samples were collected at normal harvest. Wheat forage was collected at the 6-8 

inch stage to stem elongation (jointing) stage. Wheat hay was harvested at the early flower 

(boot) to soft dough stage and allowed to field dry for 1-8 days prior to collection. Wheat grain 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 19 of 37

and straw were harvested at maturity. Sorghum forage was harvested at the soft dough to hard 

dough stage, and grain and stover were harvested at maturity. Rice grain (kernel plus hull) and 

straw were also harvested at maturity.

At the field trials planted with sweet corn varieties, corn kernel and cobs with husks removed 

(K+CWHR) were collected at commercial maturity. Forage was collected prior to the harvest of 

K+CWHR, and stover was collected at maturity following collection of K+CWHR. Additional

fresh corn samples were harvested at the milk stage from 5 of the field corn sites to represent 

K+CWHR samples. Corn forage was harvested at the late dough/early dent stage from the field 

corn trials. Corn grain and stover from the field corn trials were harvested at maturity.

All samples were analyzed for triticonazole using LC/MS/MS Method 562/0. The method is 

adequate for data collection and was validated using wheat forage, hay, grain and straw; corn 

forage, K+CWHR, grain and stover; sorghum forage, grain, and stover; and rice grain and straw. 

The validated method LOQ was 0.01 ppm in/on all tested cereal grain RACs.

Samples were also analyzed for the triazole-related metabolites, triazole (T), triazolylacetic acid 

(TAA), and triazolylalanine (TA) using LC/MS/MS Methods D0508 and D0604 (grain). The 

validated method LOQ was 0.05 ppm for each analyte in all cereal RACs, except for T and TAA 

in grain and K+CWHR, which had LOQs of 0.01 ppm each.

Prior to the analysis for triticonazole residues, samples were stored frozen for maximum 

durations of 10.6 months for wheat forage, hay, grain and straw, 11.3 months for corn, forage, 

grain, stover and K+CWHR, 8.1 months for sorghum forage, grain and stover, and 7.4 months 

for rice grain and straw. Adequate storage stability data are available for wheat matrices which 

may be translated to corn, sorghum and rice matrices, and will support the storage conditions and 

durations of samples from the cereal grain field trials.

Prior to the analysis for triazole residues, samples were stored frozen for maximum durations of 

8.8-10.6 months for wheat forage, hay, grain and straw, 6.3-10.9 months for corn, forage, grain, 

stover and K+CWHR, 5.1-8.2 months for sorghum forage, grain and stover, and 5.8-6.0 months 

for rice grain and straw. Storage stability of the triazole-related metabolites (T, TA, and TAA) 

has been demonstrated in apple, wheat flour, peanut butter, milk, and eggs stored frozen for up to 

12 months. Because T, TA, and TAA appear reasonably stable, data for wheat flour may

tentatively be translated to support the storage conditions and durations of wheat, corn, sorghum, 

and rice grain samples from the subject field trials. The stability confirmation will be assessed 

when the final report of an ongoing study investigating the storage stability of T, TA and TAA in 

canola seed, turnip roots, mustard greens, and wheat straw, grain, and forage is submitted and 

reviewed

The results of the field trials are summarized below in Table 6.

Table 6. Summary of Residue Data from Crop Field Trials with Triticonazole.

Commodity Applic.

Rate

(g ai/100

kg seed)

DAP

(days)

Residue Levels (ppm)1

n Min. Max. HAFT2 Median

(STMdR)

Mean

(STMR)

Std. 

Dev. 3
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 20 of 37

Table 6. Summary of Residue Data from Crop Field Trials with Triticonazole.

Commodity Applic.

Rate

(g ai/100

kg seed)

DAP

(days)

Residue Levels (ppm)1

n Min. Max. HAFT2 Median

(STMdR)

Mean

(STMR)

Std. 

Dev. 3

Proposed Rate on Barley, Corn (Field and Pop), Oats, Rye, Wheat, and Triticale = 0.050 lb ai/100 lb seed

Proposed Rate on Sweet Corn = 0.010 lb ai/100 lb seed

Proposed Rate on Sorghum = 0.020 lb ai/100 lb seed)

Triticonazole

Wheat, forage 50 35-102 30 <0.01 0.10 0.10 0.02 0.04 0.028

Wheat, hay 50 56-135 30 <0.01 0.08 0.08 0.02 0.03 0.026

Wheat, grain 50 90-177 30 <0.01 <0.01 <0.01 0.01 0.01 NA

Wheat, straw 50 90-177 30 <0.01 0.08 0.08 0.03 0.03 0.024

Corn, forage 450 78-155 38 <0.01 <0.01 <0.01 0.01 0.01 NA

Corn, grain 50 112-161 30 <0.01 <0.01 <0.01 0.01 0.01 NA

Corn, stover 450 92-176 38 <0.01 <0.01 <0.01 0.01 0.01 NA

Corn, K+CWHR 50 71-114 18 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, forage 50 61-113 24 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, grain 50 90-199 24 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, stover 50 90-199 24 <0.01 <0.01 <0.01 0.01 0.01 NA

Rice, grain 50 114-182 24 <0.01 0.02 0.02 0.01 0.01 0.003

Rice, straw 50 114-182 24 <0.01 0.04 0.04 0.01 0.02 0.009

Triazole (T)

Wheat, forage 50 35-102 30 <0.05 <0.05 <0.05 0.05 0.05 NA

Wheat, hay 50 56-135 30 <0.05 <0.05 <0.05 0.05 0.05 NA

Wheat, grain 50 90-177 30 <0.01 <0.01 <0.01 0.01 0.01 NA

Wheat, straw 50 90-177 30 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, forage 450 78-155 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, grain 50 112-161 30 <0.01 <0.01 <0.01 0.01 0.01 NA

Corn, stover 450 92-176 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, K+CWHR 50 71-114 18 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, forage 50 61-113 24 <0.05 <0.05 <0.05 0.05 0.05 NA

Sorghum, grain 50 90-199 24 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, stover 50 90-199 24 <0.05 <0.05 <0.05 0.05 0.05 NA

Rice, grain 50 114-182 24 <0.01 <0.01 <0.01 0.01 0.01 NA

Rice, straw 50 114-182 24 <0.05 <0.05 <0.05 0.05 0.05 NA

Triazolylalanine (TA)

Wheat, forage 50 35-102 30 <0.05 0.12 0.11 0.05 0.05 0.015

Wheat, hay 50 56-135 30 <0.05 0.14 0.13 0.05 0.06 0.021

Wheat, grain 50 90-177 30 <0.05 0.26 0.24 0.06 0.10 0.063

Wheat, straw 50 90-177 30 <0.05 0.07 0.07 0.05 0.05 0.004

Corn, forage 450 78-155 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, grain 50 112-161 30 <0.05 0.35 0.31 0.05 0.07 0.065

Corn, stover 450 92-176 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, K+CWHR 50 71-114 18 <0.05 0.26 0.24 0.05 0.07 0.061

Sorghum, forage 50 61-113 24 <0.05 0.10 0.08 0.05 0.05 0.010

Sorghum, grain 50 90-199 24 <0.05 0.39 0.38 0.05 0.08 0.091

Sorghum, stover 50 90-199 24 <0.05 0.26 0.25 0.05 0.07 0.057

Rice, grain 50 114-182 24 <0.05 0.29 0.29 0.05 0.07 0.066

Rice, straw 50 114-182 24 <0.05 <0.05 <0.05 0.05 0.05 NA
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 21 of 37

Table 6. Summary of Residue Data from Crop Field Trials with Triticonazole.

Commodity Applic.

Rate

(g ai/100

kg seed)

DAP

(days)

Residue Levels (ppm)1

n Min. Max. HAFT2 Median

(STMdR)

Mean

(STMR)

Std. 

Dev. 3

Triazolyl-acetic acid (TAA)

Wheat, forage 50 35-102 30 <0.05 <0.05 <0.05 0.05 0.05 NA

Wheat, hay 50 56-135 30 <0.05 0.13 0.11 0.05 0.06 0.016

Wheat, grain 50 90-177 30 <0.01 0.20 0.20 0.04 0.05 0.046

Wheat, straw 50 90-177 30 <0.05 0.10 0.10 0.05 0.05 0.011

Corn, forage 450 78-155 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, grain 50 112-161 30 <0.01 <0.01 <0.01 0.01 0.01 NA

Corn, stover 450 92-176 38 <0.05 <0.05 <0.05 0.05 0.05 NA

Corn, K+CWHR 50 71-114 18 <0.01 <0.01 <0.01 0.01 0.01 NA

Sorghum, forage 50 61-113 24 <0.05 0.07 0.06 0.05 0.05 0.004

Sorghum, grain 50 90-199 24 <0.01 0.37 0.35 0.01 0.04 0.095

Sorghum, stover 50 90-199 24 <0.05 <0.05 <0.05 0.05 0.05 NA

Rice, grain 50 114-182 24 <0.01 0.50 0.50 0.03 0.07 0.134

Rice, straw 50 114-182 24 <0.05 0.34 0.34 0.05 0.08 0.082

1

 For statistical calculations, the LOQ (0.01 ppm for triticonazole; 0.05 ppm for each triazole analyte, except for T and TAA in 

corn grain and K+CWHR which have a LOQ of 0.01 ppm each) was used for all values reported below the LOQ. 

2

 HAFT = Highest Average Field Trial.

3

 Standard deviation is not applicable (NA) when all samples have residues below the LOQ.

4

 Represented by 8 sweet corn samples, and 30 field corn samples.

Corn forage samples from the sweet corn trials were harvested prior to collection of K+CWHR 

from 3 trials and 50 days after in the other trial, and corn forage from the 5 field corn trials, 

which also collected fresh corn to represent K+CWHR and bridge sweet corn requirements, was 

harvested 1-5 weeks after samples of fresh corn at the milk dough stage. The field corn forage 

samples do not actually represent sweet corn forage typically collected prior to or with 

K+CWHR; however, given that residues of triticonazole were nonquantifiable in all corn forage 

samples and application was made to seed prior to planting, no additional sweet corn forage 

samples will be required.

Conclusions. The submitted residue data are adequate to support the requested seed treatment 

uses for cereal grain crops (except rice) pending submission of the final study report from an 

ongoing triazole-related residues storage stability study. There are adequate data for the 

representative crops of the cereal grain groups 15 and 16 to establish crop group or individual 

tolerances. The number and locations of field trials were conducted according to the 860.1500 

guidelines. The seed treatment rate (0.05 lb ai/100 lbs seed) used in the field trials reflects the 

proposed use of Triticonazole HL Fungicide (EPA Reg. No. 7969-xxx) on barley, field corn and 

popcorn, oats, rye, wheat, and triticale. However, the rate reflects 5x and 2.5x the proposed seed 

treatment rate for sweet corn and sorghum, respectively. 

Residues of triticonazole were below the LOQ of 0.01 ppm in treated wheat grain, corn grain, 

corn K+CWHR, and sorghum grain. It is noted that the seed treatment rate used for sweet corn 

and sorghum were exaggerated. Low but quantifiable residues of triticonazole were observed in 

rice grain. However, because the petitioner is excluding the use of Triticonazole HL Fungicide

on rice, the available data will support a tolerance at the LOQ (0.01 ppm) for the cereal grains, 

group 15 (except rice).
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 22 of 37

Residues of triticonazole were also nonquantifiable in treated field and sweet corn forage and 

stover, and sorghum forage and stover. Quantifiable residues of triticonazole were observed in 

treated wheat forage, hay and straw, and rice straw. The appropriate tolerance levels were 

statistically calculated (see Appendix I) according to Guidance for Setting Pesticide Tolerances 

Based on Field Trial Data SOP. The residue data for rice straw was excluded from the 

spreadsheet because the petitioner is not applying for use on rice. The tolerance spreadsheet 

calculated tolerances of 0.20 ppm for wheat forage, 0.25 ppm for wheat hay, and 0.20 ppm for 

wheat straw. Based on the maximum residue determined for the representative crops, HED 

recommends a crop group 16 tolerance for triticonazole of 0.25 ppm. A revised Section F must 

be submitted to reflect the tolerance recommendation for the forage, fodder, and straw of cereal 

grain, group 16.

The residue data and tolerances recommended in this document will also support the currently

registered seed treatment uses of Charter® Fungicide Treatment (EPA Reg. No. 7969-386) for 

preplant seed treatment on barley and wheat.

860.1520 Processed Food and Feed

DER Reference: 47511901.der.doc

BASF has submitted a processing study for triticonazole with field corn, rice, sweet sorghum, 

and wheat. Four field trials were conducted (one field trial for each representative crop) in the 

United States during the 2007 growing season. At each test location, cereal grain crops were 

grown from seeds treated with a 4.2 lb/gal flowable liquid formulation of triticonazole, prior to 

planting, at 250 g ai/100 kg seed (4 oz ai/100 lb seed; 5x the proposed seed treatment rate). The 

triticonazole formulation was mixed with Apron XL (mefenoxam; a seed fungicide) and a 

colorant. A separate plot (untreated) was planted with seed treated with Apron XL and colorant 

only. The application made to sorghum seed additionally included pyraclostrobin; the 

pyraclostrobin data are not reviewed herein. 

Samples of field corn, rice and wheat grain, and sweet sorghum stalks were collected at maturity

and processed using procedures simulating commercial practices for corn grits, meal, oil (dry and 

wet milled), starch and flour; rice hulls, bran and polished rice; sorghum syrup; and wheat bran, 

flour, middlings, shorts, and germ.

All samples were analyzed for triticonazole using LC/MS/MS Method 562/0. The method was 

adequate for data collection based on acceptable concurrent method recoveries with wheat grain, 

corn grain, sorghum stalk, and rice grain, bran, hulls and polished rice. The validated method 

LOQ was 0.01 ppm in/on all tested cereal grain RACs and processed matrices.

Samples were also analyzed for the triazole-related metabolites, triazole (T), triazolyl-acetic acid 

(TAA) and triazolylalanine (TA) using LC/MS/MS Methods D0508 and D0604 (grain). The 

validated method LOQ was 0.01 ppm for T in wheat grain, bran, and flour; corn grain oil and 

starch; sorghum stalks; and rice grain, bran, hulls and polished rice, and TAA in wheat grain, 

bran, germ, middlings, shorts and flour; corn grain, flour, grits, meal, oil and starch; sorghum 

stalks and syrup; and rice grain, bran, hulls and polished rice. The validated LOQ for TA was 

0.05 ppm in wheat grain, bran, germ, middlings, shorts and flour; corn grain, flour, grits, meal, 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 23 of 37

oil and starch; sorghum stalks and syrup; and rice grain, bran, hulls, and polished rice.

RAC samples were stored frozen from harvest to analysis for triticonazole residues for up to 6.7 

months for corn grain, rice grain, sorghum stalks, and wheat grain. The only processed 

commodities analyzed for triticonazole residues were stored frozen from collection to analysis 

for up to 1 month for rice hulls, 1.5 months for wheat bran, and 6.7 months for polished rice. 

Storage stability data for triticonazole are available for wheat grain, forage, hay, and straw, and 

processed wheat germ, flour and bran which may be translated to support the storage durations of 

corn, rice, and wheat grain, sorghum stalks, and rice bran from the subject processing studies.

The maximum storage durations of RAC samples from harvest to analysis of triazole residues 

were 10.4 months for corn, rice and wheat grain and 5.9 months for sorghum stalks. Because T, 

TA, and TAA appear relatively stable, data for wheat flour may be translated to support the 

storage conditions and durations of wheat, corn, sorghum, and rice grain (RAC) samples from the 

subject processing study. A final report for stability of T, TA and TAA in canola seed, turnip 

roots, mustard greens, and wheat straw, grain, and forage is to be submitted upon completion.

The maximum storage durations for processed matrices from collection to analysis for triazole 

metabolites were 0.3-0.4 months for corn oil and starch; 1 month for rice bran and 1.2-1.3 

months for hulls and polished rice; 0.7 months for sorghum syrup; and 1.8-2.3 months for corn 

flour, grits and meal, and wheat bran, flour, middlings, shorts and germ. The submitted storage 

stability data for wheat flour will support the storage durations of corn and wheat flour samples 

and may be translated to support polished rice samples. Supporting storage stability data are not 

required for corn oil and starch; rice bran; and sorghum syrup as these matrices were analyzed

within 30 days of processing. Supporting storage stability data are, however, required for T, TA 

and TAA in frozen rice hulls, corn grits and meal, and wheat bran, middlings, shorts, and germ.

The processing data for field corn, rice, sweet sorghum, and wheat showed that residues of 

triticonazole were nonquantifiable (<0.01 ppm) in the RACs (corn, rice, and wheat grain or 

sorghum stalk) grown from seeds treated at 250 g triticonazole/100 kg seed. Corn, sorghum and 

wheat processed matrices were, therefore, not analyzed. However, rice processed matrices were 

analyzed because some quantifiable residues were detected in the field trials conducted at the 

nominal rate (see 47470203.der.doc). Residues of triticonazole were <0.01 ppm in polished rice, 

0.025-0.031 ppm in hulls, and 0.010-0.011 ppm in bran. Based on the results of the rice 

processing study, residues of triticonazole may concentrate in hulls (2.8x) but do not appear to 

significantly concentrate in bran (1.1x). Processing factors in polished rice could not be 

calculated because residues were nonquantifiable in both the RAC and processed matrix.

Residues of the triazole-related metabolite T were nonquantifiable (<0.01 ppm) in corn, rice and 

wheat grain, and sorghum stalks (RACs) and the processed commodities of wheat bran and flour, 

corn oil (dry and wet milled) and starch, and rice hulls, bran and polished rice. Processing 

factors could not be calculated for these matrices, and analysis of all processed matrices was not 

required being that T residues were nonquantifiable in the respective RAC samples. 

Residues of the triazole-related metabolite TA were nonquantifiable (<0.05 ppm) in sorghum 

stalks and processed syrup; therefore, processing factors could not be calculated. However, 

residues of TA were quantifiable in wheat grain (0.08 ppm), corn grain (0.08-0.09 ppm), and rice 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 24 of 37

grain (0.06-0.07 ppm). Residues of TA were 0.18-0.20 ppm in wheat bran, <0.05 ppm in flour, 

0.06 ppm in middlings, 0.08 ppm in shorts, and 0.13-0.23 ppm in germ; 0.052-0.060 ppm in corn 

grits, 0.098-0.105 ppm in meal, and <0.05 ppm in oil (dry and wet milled), starch and flour; and 

<0.05 ppm in rice hulls and polished rice, and 0.39-0.40 ppm in bran. Based on the results of the 

wheat, corn and rice processing studies, residues of TA do not concentrate in wheat flour (0.6x), 

middlings (0.8x) and shorts (1x); corn grits (0.6x), oil (dry and wet milled; 0.6x), starch (0.6x), 

flour (0.6x), and meal (1.1x); and rice hulls (0.7x) and polished rice (0.7x). Residues of TA 

appear to concentrate in wheat bran (2.4x) and germ (2.3x); and rice bran (5.7x).

Residues of the triazole-related metabolite TAA were nonquantifiable (<0.01 ppm) in corn grain 

and sorghum stalks (RACs) and processed corn grits, meal, oil (dry and wet milled), starch and 

flour, and sorghum syrup, therefore, processing factors could not be calculated. However, 

residues of TAA were quantifiable in wheat grain (0.03 ppm) and rice grain (0.10 ppm). 

Residues of TAA were 0.04 ppm in wheat bran, 0.02-0.03 ppm in flour, 0.03 ppm in middlings, 

0.03 ppm in shorts, and 0.03 ppm in germ; and 0.05-0.12 ppm in rice hulls, 0.05-0.06 ppm in 

polished rice, and 0.58-0.65 ppm in rice bran. Based on the results of the wheat and rice 

processing studies, residues of TAA do not concentrate in wheat flour, middlings, shorts, and 

germ (1x); and rice hulls (0.9x) and polished rice (0.6x). Residues of TAA appear to concentrate 

in rice bran (6.2x) and only slightly in wheat bran (1.3x).

The observed processing factors of triticonazole in rice hulls (2.8x) and bran (1.1x) were below 

the theoretical concentration factors (860.1520, Table 3; based on separation into components) of 

5x for rice hulls and 7.7x for rice bran. The theoretical concentration factors (860.1520; Table 3) 

for other cereal grain matrices tested are 25x for corn oil, 7.7x for wheat bran, 1.4x for wheat 

flour, and 8.3x for wheat shorts. No theoretical concentration factors are cited for sweet sorghum 

in 860.1520.

A summary of the processing factors for triticonazole and the triazoles TA and TAA is presented 

in Table 7. T is not included because residues were nonquantifiable in the RAC and all 

processed matrices tested.

Table 7. Summary of Processing Factors for Triticonazole and Triazoles.

RAC Processed Commodity Processing Factor 1

Triticonazole TA TAA

Wheat Bran NA 2.4x 1.3x

Flour NA 0.6x 1x

Middlings NA 0.8x 1x

Shorts NA 1x 1x

Germ NA 2.3x 1x

Corn Grits NA 0.6x NC

Meal NA 1.1x NC

Oil, dry milled NA 0.6x NC

Oil, wet milled NA 0.6x NC

Starch NA 0.6x NC

Flour NA 0.6x NC

Sorghum, sweet Syrup NA NC NC
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 25 of 37

Table 7. Summary of Processing Factors for Triticonazole and Triazoles.

RAC Processed Commodity Processing Factor 1

Triticonazole TA TAA

Rice Polished rice NC 0.7x 0.6x

Hulls 2.8x 0.7x 0.9x

Bran 1.1x 5.7x 6.2x

1 NA = Not analyzed because residues were nonquantifiable in the RAC at nominal and exaggerated rates.

 NC = Not calculated because residues were nonquantifiable in both the RAC and processed matrix.

Conclusions. The processing data for field corn, rice, sweet sorghum, and wheat are adequate 

pending submission of additional supporting freezer storage stability data for T, TA, and TAA in 

rice hulls, corn grits and meal, and wheat bran, middlings, shorts, and germ for up to 2 months. 

The results indicate that residues of triticonazole were nonquantifiable (<0.01 ppm) in the cereal 

grain RACs (corn, rice, wheat, and sorghum) grown from seeds treated at 250 g triticonazole/100 

kg seed (5x the maximum proposed seed treatment rate). The processed corn, sorghum, and 

wheat matrices were not further analyzed. Analysis of rice processed matrices showed that 

residues of triticonazole may concentrate in hulls (2.8x) but not significantly in bran (1.1x); 

residues in polished rice were nonquantifiable. As the petitioner is not seeking to register use on 

rice at this time, no tolerances are required for rice processed matrices. However, based on the 

HAFT for rice grain (0.02 ppm) and processing factors of 2.8x for hulls, the maximum expected 

triticonazole residues would be 0.06 ppm in rice hulls.

860.1650 Submittal of Analytical Reference Standards

Analytical standards for triticonazole are currently available in the EPA National Pesticide 

Standards Repository (personal communication with Dallas Wright, ACB, 2/23/09), expiration 

date 7/8/2014. 

860.1850 Confined Accumulation in Rotational Crops

DER Reference: 47399201.der.doc

Residue Chemistry Memo DP# 262566, 6/6/2001, N. Dodd (PP#9F6051)

BASF has submitted a confined rotational crop study with [14C-phenyl]triticonazole and [3(5)-

14C-triazole]triticonazole. The study was submitted in support of the current petition and in 

response to the condition of registration requirement under PP# 9F6051 (DP# 262566, 6/6/2001, 

N. Dodd). Each radiolabeled test substance was applied as a solution in methanol to bare soil in 

pots maintained in a greenhouse at a nominal rate of 0.107 lb ai/A. After soil treatment, 

rotational crops consisting of lettuce, radish, and spring wheat were planted 30, 120 and 365 

days. Lettuce, radish roots and tops, and wheat grain, chaff and straw were harvested at maturity,

and wheat forage was harvested 61-63 days after planting (DAP).

Total radioactive residues (TRR) were determined for the rotated crops; see Table 8 below. TRR 

ranged from 0.003 ppm in phenyl-label radish root (365-day PBI) to 0.848 ppm in triazole-label 

wheat chaff (120-day PBI). Significantly higher TRR (by a factor of ≥3) were observed in the 

triazole-label matrices than in the respective phenyl-label matrices. TRR typically declined with 

later plantback intervals (PBIs), except in mature wheat matrices (straw, chaff, and/or grain).
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 26 of 37

Table 8. Total Radioactive Residues in Rotated Lettuce, Radish, and Wheat Matrices.

Matrix Plantback interval 

(days)

Phenyl Label (ppm) Triazole Label (ppm) 

Combustion Calculated 1 Combustion Calculated 1

Lettuce 30 0.020 0.024 0.065 0.065

120 0.014 0.014 0.026 0.025

365 0.005 0.004 0.029 0.024

Radish, roots 30 0.009 0.009 0.099 0.094

120 0.006 0.006 0.055 0.056

365 0.003 0.003 0.031 0.029

Radish, tops 30 0.072 0.067 0.279 0.256

120 0.052 0.040 0.331 0.286

365 0.011 0.009 0.128 0.043

Wheat, forage 30 0.067 0.042 0.199 0.129

120 0.049 0.035 0.155 0.095

365 0.029 0.015 0.141 0.107

Wheat, straw 30 0.209 0.181 0.629 0.580

120 0.345 0.298 0.675 0.576

365 0.282 0.198 0.520 0.455

Wheat, chaff 30 0.181 0.174 0.509 0.571

120 0.151 0.141 0.848 0.786

365 0.106 0.095 0.562 0.549

Wheat, grain 30 0.008 0.008 0.453 0.948

120 0.054 0.053 0.689 0.706

365 0.025 0.028 0.682 0.773

1 Calculated by the summation of extractable and nonextractable radioactivity; these values were used by the petitioner for all 

further calculations.

To characterize and identify radioactive residues in rotated crops, samples were sequentially

extracted and partitioned with organic solvents. The nonextractable residues in representative 

120-DAT wheat grain and straw were further released with aqueous ammonia extraction and 

enzyme hydrolysis. Initial identification of extracts and hydrolysates was accomplished by cochromatography using several HPLC systems, and confirmation of residues was made by

LC/MS/MS. TRR accountabilities were 63-120% for phenyl-label rotated crop matrices and 34-

209% for triazole-label rotated crop matrices. Accountabilities indicate that the extraction 

procedures may not have adequately released radioactivity in some rotated crop matrices, but 

TRR levels in the extracted samples were sufficient to characterize the residues in rotated crops 

matrices. 

The parent, triticonazole, was the only residue identified in phenyl-label rotated crop matrices. 

Triticonazole accounted for 4.2-15.4% TRR (<0.01 ppm) in 30-DAT lettuce, radish root and 

tops, and wheat forage and chaff, and 6.5% TRR (0.012 ppm) in 30-DAT wheat straw; 30-DAT 

wheat grain was not analyzed because extractable radioactivity was only 0.002 ppm. In 120-

DAT rotated crop samples, triticonazole accounted for 0.2-10.0% TRR (<0.01 ppm) in lettuce, 

radish tops, and wheat forage, chaff, and grain, and 8.2% TRR (0.025 ppm) in wheat straw; 120-

DAT radish root was not analyzed because extractable radioactivity was only 0.004 ppm. In 365-

DAT rotated crop samples, triticonazole accounted for 3.5-4.8% TRR (<0.01 ppm) in wheat 

straw and chaff; other 365-DAT crop matrices (lettuce, radish tops and root, wheat grain and 

forage) were not analyzed because extractable radioactivities were only 0.002-0.006 ppm.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 27 of 37

The parent, triticonazole, was also identified in all triazole-label rotated crop matrices, except 

wheat grain. Triticonazole accounted for 7.8% TRR (<0.01 ppm) in 30-DAT radish root, and 

19.2-32.1% TRR (0.017-0.133 ppm) in 30-DAT lettuce, radish tops, and wheat forage, straw and 

chaff. In 120-DAT rotated crop samples, triticonazole accounted for 5.2% and 5.5% TRR (<0.01 

and 0.016 ppm) in radish root and tops, respectively, 22.3% TRR (<0.01 ppm) in lettuce, and 

14.5-26.0% TRR (0.019-0.150 ppm) in wheat forage, straw and chaff. In 365-DAT rotated crop 

samples, triticonazole accounted for 6.6-13.6% TRR (<0.01 ppm) in lettuce, radish root, and 

radish tops, and 18.9-24.1% TRR (0.025-0.133 ppm) in wheat forage, straw and chaff.

Triazole-related metabolites, TA and TAA, were identified in triazole-label rotated crop 

matrices. TA accounted for 1.0-6.4% TRR (0.004-0.017 ppm) in 30-DAT lettuce, wheat straw, 

and wheat grain, and 10.8-40.4% TRR (0.028-0.103 ppm) in radish root and tops, and wheat 

forage and grain. TAA was only identified in 30-DAT lettuce at 12.2% TRR (0.008 ppm) and 

wheat straw, chaff, and grain at 17.1-29.8% TRR (0.139-0.173 ppm). In 120-DAT rotated crop 

samples, TA residues increased in most mature matrices and accounted for 13.2% TRR (0.003 

ppm) in lettuce, and 6.8-44.4% TRR (0.013-0.313 ppm) in radish root and tops, and wheat 

forage, chaff and grain; TA was not detected in 120-DAT wheat straw. In 365-DAT rotated crop 

samples, TA residues increased in all matrices, except wheat grain, and accounted for 21.7-

24.8% TRR (0.005-0.007 ppm) in lettuce and radish root, 34.7-40.7% TRR (0.015-0.044 ppm) in 

radish tops and wheat forage, and 8.5-8.8% TRR (0.048-0.066 ppm) in wheat chaff and grain; 

TA was detected at low levels (2.0% TRR, 0.009 ppm) in 365-DAT wheat straw. TAA was not 

detected in any 120- or 365-DAT samples.

An unknown polar peak, accounting for ~5-18% TRR (0.002-0.059 ppm), was detected in all 

triazole-label rotated crop matrices, except wheat grain. The unknown peak generally declined 

with later plantback intervals. LC/MS/MS of the aqueous phase of the methanol extract of radish 

top and ethyl acetate phase of the methanol extract of wheat straw confirmed no significant 

isotope pattern and no evidence for free triazole in the unknown peak. The unknown was 

characterized as a polar cluster consisting of more than 25 peaks at negligible levels; however, 

quantitative data were not provided. The remaining extractable residues were characterized as 

polar, medium polar, or nonpolar peaks accounting for ~1-16% TRR (≤0.001 to ≤0.016 ppm) in 

phenyl-label samples and ~1-29% TRR (≤0.001 to ≤0.027 ppm) in triazole label-samples.

All samples and extracts were stored frozen prior to analysis. Based on the analysis dates 

obtained from the HPLC chromatograms, the majority of rotated crop samples were initially

analyzed within six months of harvest. Several samples (i.e., 30-DAT lettuce, radish roots and 

tops, and wheat forage, straw and chaff; 120-DAT wheat straw; and 365-DAT lettuce, wheat 

forage and chaff) were stored for up to 9.6 months prior to initial HPLC analysis. HPLC 

analyses and co-chromatography to further characterize and confirm metabolite identifications 

were conducted on samples/extracts stored up to ~18 months. The dates of sample extraction, 

partitioning, and further hydrolysis of nonextractable wheat grain and straw samples were not 

reported.

Re-analysis of methanol extracts of phenyl-label lettuce, radish root, radish tops and spring wheat 

(30-DAT), and triazole-label lettuce, radish root and wheat grain (30-DAT) demonstrate similar 

metabolic profiles in the re-analyzed (~13 months after initial analysis) and initial sample 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 28 of 37

extracts. Re-extraction and re-analysis of phenyl-label radish top and triazole-label radish root 

and wheat grain samples (~14-15 months after the initial analysis) also demonstrate similar 

extractability and metabolic profiles in the re-extracted and initial samples. Given that the 

majority of samples appear to have been analyzed within 6 months, the available storage stability

data will be considered adequate to support the subject study.

Based on the submitted confined rotational crop study, HED concludes that metabolism of 

triticonazole in rotational crops involved degradation of triticonazole in the soil resulting in the 

formation of free triazole. As a very polar compound, free triazole is easily taken up by the 

plants and transformed to triazolylalanine (TA), which is further metabolized to triazolylacetic 

acid (TAA) and other polar conjugates. The occurrence of residual radioactivity indicates that a 

portion of residues was incorporated and/or associated into natural products, such as starch, 

cellulose and/or lignins.

Conclusions. The confined rotational crop study is acceptable. The application rate used reflects

1.4x the equivalent per acre rate proposed for cereal grain crops. The metabolism of triticonazole 

in rotated crops appears similar to those observed for primary crops, except that triazole-related 

metabolites were detected in the rotated crops. The data indicate that residues of triticonazole 

may be present at >0.01 ppm in 30-DAT rotated lettuce (0.017 ppm), radish tops (0.049 ppm) 

and wheat forage (0.041 ppm), straw (0.133 ppm) and chaff (0.139 ppm); 120-DAT rotated 

radish tops (0.016 ppm), and wheat forage (0.019 ppm), straw (0.150 ppm), and chaff (0.114 

ppm); and 365-DAT rotated wheat forage (0.025 ppm), straw (0.086 ppm) and chaff (0.133 

ppm). Wheat hay was not analyzed but forage and straw were analyzed, and residues of 

triticonazole were found in forage and straw.

860.1900 Field Accumulation in Rotational Crops

The proposed label for the 4.17 lb/gal FS specifies that “planting of any crop not listed on the 

label within 30 days after planting treated seed is not allowed”. The results of the submitted 

confined rotational crop study do not support this restriction. The data from the confined 

rotational crop study indicate that triticonazole residues greater than 0.01 ppm were detected at 

the 30-day PBI. To support a 30-day plantback restriction on the label, limited field rotational 

crop data for leafy vegetables, root and tuber vegetables, foliage of root and tuber vegetables, and 

a cereal grain are required. Until the requested data are provided, the label must be modified to 

specify a 120-day (4-month) plantback restriction for leafy vegetables, a 365-day (12-month) 

plantback restriction for root and tuber vegetables, and prohibit the planting of all other crops not 

listed on the label.

Although triticonazole was detected at >0.01 ppm in rotated wheat forage, straw, and chaff 

planted at the 30-, 120- and 365-day PBIs, the levels of triticonazole found in the confined 

rotational crop studies were below the recommended tolerance of 0.25 ppm for the forage, 

fodder, and straw of cereal grain crop group. Therefore, residues which may be found in rotated 

cereal grain crops (foliage) in the confined rotational crop study would not be expected to exceed 

the recommended tolerances for triticonazole. Nevertheless, HED continues to request data on a 

rotated cereal grain because the cereal grain represents other crops and residues in wheat as a 

rotational crop are often the highest.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 29 of 37

860.1550 Proposed Tolerances

Tolerances have been established under 40 CFR §180.583(a) for residues of the fungicide 

triticonazole, (1RS)-(E)-5-[(4-chlorophenyl)methylene]-2,2-dimethyl-1-(1H -1,2,4-triazol-1-

ylmethyl)cyclopentanol, from the treatment of seed prior to planting in/on barley grain, hay and 

straw, and wheat grain, forage, hay and straw, each at 0.05 ppm. HED has previously determined 

that triticonazole is the residue of concern in barley and wheat from seed treatment uses. This 

regulatory decision may be extended to include all crop commodities in the cereal grains crop 

group. The petitioner has not specified the tolerance expression with the proposed tolerances in 

Section F of the petition. 

The submitted residue data for field corn grain, sweet corn K+CWHR, sorghum grain, and wheat 

grain, which are the representative commodities of the cereal grain group, do not support the 

proposed tolerance for the ‘Grain, cereal, group 15 (except rice)’ at 0.05 ppm. The data do 

support a tolerance for ‘Grain, cereal, group 15 (except rice)’ at 0.01 ppm. A revised Section F 

must be submitted to reflect the tolerance recommendation of 0.01 ppm for “Grain, cereal, group 

15 (except rice)” at 0.01 ppm. The established individual tolerances for wheat grain and barley

grain must be deleted when the crop group tolerance is established.

The submitted residue data for corn forage and stover, sorghum forage and stover, and wheat 

forage, hay, and straw are adequate to determine a tolerance level for the forage, fodder, and 

straw of cereal grains crop group (except rice). Since the residues in wheat commodities were 

highest, the tolerance levels for wheat forage, wheat hay, and wheat straw were statistically

calculated (see Appendix I) according to Guidance for Setting Pesticide Tolerances Based on 

Field Trial Data SOP (January 2008 version). Because >10% of the residues were below the 

LOQ (<0.01 ppm), the maximum likelihood estimation (MLE) procedures were needed to impute 

censored values. The tolerance spreadsheet calculated tolerances of 0.20 ppm for wheat forage, 

0.25 ppm for wheat hay, and 0.20 ppm for wheat straw. HED recommends a crop group 

tolerance of 0.25 ppm for the forage, fodder, and straw of the cereal grain crop group, group 16, 

based on the highest calculated value for the wheat commodities (0.25 ppm for wheat hay). 

A revised Section F must be submitted to reflect the tolerance recommendation of 0.25 ppm for 

“Grain, cereal, forage, fodder, and straw, group 16 (except rice)” as shown in Table 9. The 

established individual tolerances for barley hay and straw, and wheat forage, hay and straw must 

be deleted when the crop group tolerance is established.

The processing data for corn, sorghum, and wheat showed no concentration of triticonazole 

residues following seed treatment at 5x. The processing data for rice showed that residues of 

triticonazole concentrated in hulls (2.8x) but not significantly in bran (1.1x). As rice is excluded 

from the proposed seed treatment uses on cereal grains, no tolerances are required for the 

processed commodities of cereal grains for the purpose of this action.

Based on the available cow and poultry metabolism studies, and low levels of residues found in 

the cereal grain field trials, there is no reasonable expectation that quantifiable residues of 

triticonazole will occur in livestock commodities [40 CFR §180.6(a)(3)]. Therefore, livestock 

tolerances are not required at this time. 
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 30 of 37

A summary of the recommended tolerances for the petition is listed in Table 9. There are no 

Codex or Mexican MRLs established for residues of triticonazole. Canadian MRLs are 

established for triticonazole per se in/on barley, wheat and oats each at 0.01 ppm. 

Table 9. Tolerance Summary for Triticonazole

Commodity Established/Proposed

Tolerance (ppm)

Recommended 

Tolerance (ppm)

Comments; Correct Commodity 

Definition

Established tolerances under 180.583(a)

Barley, grain 0.05 Remove Barley grain will be covered with the 

establishment of the cereal grain, 

group 15 (except rice) tolerance.

Barley, hay 0.05 Remove Increase in the tolerance based on 

residues resulting from new uses.

Barley hay and straw and wheat 

forage will be covered with the 

establishment of the cereal grain 

group 16 (except rice) tolerance.

Barley, straw 0.05 Remove

Wheat, forage 0.05 Remove

Wheat grain 0.05 Remove Barley grain will be covered with the 

establishment of the cereal grain, 

group 15 (except rice) tolerance

Wheat, hay 0.05 Remove Increase in the tolerance based on 

residues resulting from new uses. 

Wheat hay and straw and will be 

covered with the establishment of the 

cereal grain group 16 (except rice) 

tolerance.

Wheat, straw 0.05 Remove

Tolerances to be established under 180.583(a)

Grain, cereal, group 15 

(except rice)

0.05 0.01 .

Grain, cereal, forage, 

fodder, and straw, group 

16 (except rice)

0.10 0.25 .
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 31 of 37

References

DP#: 270496

Subject: PP#9F6051. Triticonazole on Barley and Wheat. Method Validation Request.

From: N. Dodd

To: F.D. Griffith

Dated: 12/7/2000

MRIDs: 44904518, 44904519

DP#: 262566

Subject: PP#9F6051. Triticonazole Seed Treatment on Barley and Wheat. Review of 

Analytical Methods and Residue Data. First Food Use Review.

From: N. Dodd

To: S. Gardner-Jenkins/M. Waller

Dated: 6/6/2001

MRIDs: 44904515-25, 44995701-06

DP#: 322215

Subject: 1,2,4-Triazole, Triazole Alanine, Triazole Acetic Acid: Human Health Aggregate 

Risk Assessment in Support of Reregistration and Registration Actions for 

Triazole-derivative Fungicide Compounds.

From: M. Doherty; K. Raffaele; K. Farwell; S. Dapson; K. Schumacher; J. Arthur; D. 

Hrdy; I. Maher

To: T. Gibson/C. Giles Parker; M. Goodis; S. Lewis

Dated: 2/7/2006

MRIDs: None

DP#: 303508 & 314517

Subject: PP#4F6830. Prothioconazole. Petition for Establishment of Tolerances for Use 

on Barley, Oilseed (Except Sunflower and Safflower) Crop Group, Dried Shelled 

Pea and Bean (Except Soybean) Crop Subgroup, Peanut, Rice, and Wheat. 

Summary of Analytical Chemistry and Residue Data.

From: S. Funk

To: L. Coppolino

Date: 8/21/06

MRIDs: 46246139, 46246141-50, 46246201-11, 46246213-27, 46477701-04

Attachments: 

International Residue Limit Status sheet

Appendix I - Tolerance Assessment Calculations

Template Version September 2005
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 32 of 37

INTERNATIONAL RESIDUE LIMIT STATUS

Chemical Name: (1RS)-

(E)-5-[4-chlorophenyl)

methylene]-2,2-dimethyl1-(1H-1,2,4-triazol1ylmethyl)cyclopentanol

Common Name:

Triticonazole

X Proposed tolerance

 Reevaluated tolerance

 Other

Date: 02/2009

Codex Status (Maximum Residue Limits) U. S. Tolerances

X No Codex proposal step 6 or above

 No Codex proposal step 6 or above for the crops 

requested

Petition Number: PP#8F7420

DP#: 360624

Other Identifier: Decision # 399232 

Residue definition (step 8/CXL): N/A Reviewer/Branch: P. Deschamp, A. Acierto/RAB3

Residue definition: triticonazole per se

Crop (s) MRL (mg/kg) Crop(s) Tolerance (ppm)

Grain, cereal, group 15 (except 

rice)

0.05

Grain, cereal, forage, fodder 

and straw, group 16 (except 

rice)

0.10

Limits for Canada Limits for Mexico

 No Limits

 No Limits for the crops requested

X No Limits

 No Limits for the crops requested

Residue definition: 5-[(4-chlorophenyl)methylene]-2,2-

dimethyl-1-(1H-1,2,4-triazol-1-ylmethyl)cyclopentanol

Residue definition: N/A

Crop(s) MRL (mg/kg) Crop(s) MRL (mg/kg)

Barley 0.01

Wheat 0.01

Oats 0.01

Notes/Special Instructions: S.Funk, 02/19/2009.
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 33 of 37

Appendix I. Tolerance Assessment Calculations.

Wheat

The dataset used to establish tolerances for triticonazole on wheat forage, hay, and straw 

consisted of field trial data representing application rates of 50 g ai/100 kg seed (seed treatment), 

with samples collected at typical commercial harvest. As specified by the Guidance for Setting 

Pesticide Tolerances Based on Field Trial Data (SOP), the field trial application rates are within 

25% of the maximum label application rate. The residue values that were entered into the 

tolerance spreadsheet are provided in Table I-1.

Because 4 of 30 field trial sample results for wheat forage, 8 of 30 field trial sample results for 

wheat hay, and 6 of 30 field sample results for wheat straw were below the LOQ, maximum 

likelihood estimation (MLE) procedures were needed to impute censored values. For these 

matrices visual inspection of the lognormal probability plots (Figures I-1, I-3, and I-5) and the 

results from the approximate Shapiro-Francia test statistic (Figures I-2, I-4, and I-6) indicated

that the datasets were reasonably lognormal.

Since the field trial data for triticonazole on wheat forage, hay, and straw represent large datasets

(i.e., more than 15 samples) and are reasonably lognormal, the minimum of the 95% upper 

confidence limit (UCL) on the 95th percentile and the point estimate of the 99th percentile should 

be selected as the tolerance value. Using the rounding procedure as outlined in the SOP, the 

recommended tolerances are 0.20 ppm for wheat forage, 0.25 ppm for wheat hay, and 0.20 ppm

for wheat straw. 

Table I-1. Residue data used to calculate tolerance for Triticonazole on Wheat Forage, Hay and Straw.

Regulator: EPA EPA EPA

Chemical: Triticonazole Triticonazole Triticonazole

Crop: Wheat, forage (MLE) Wheat, hay (MLE) Wheat, straw (MLE)

PHI: Not applicable Not applicable Not applicable

App. Rate: 50 g ai/100 kg seed 50 g ai/100 kg seed 50 g ai/100 kg seed

Submitter: BASF Crop Protection BASF Crop Protection BASF Crop Protection

MRID Citation: MRID 47470203 MRID 47470203 MRID 47470203

Residues of Triticonazole (ppm)

1

0.070 0.070 0.030

0.050 0.070 0.040

0.005 0.002 0.020

0.006 0.010 0.020

0.010 0.004 0.004

0.010 0.005 0.005

0.100 0.060 0.050

0.090 0.060 0.060

0.050 0.080 0.030

0.070 0.080 0.040

0.020 0.006 0.006

0.020 0.006 0.007
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 34 of 37

Table I-1. Residue data used to calculate tolerance for Triticonazole on Wheat Forage, Hay and Straw.

Regulator: EPA EPA EPA

Chemical: Triticonazole Triticonazole Triticonazole

Crop: Wheat, forage (MLE) Wheat, hay (MLE) Wheat, straw (MLE)

PHI: Not applicable Not applicable Not applicable

App. Rate: 50 g ai/100 kg seed 50 g ai/100 kg seed 50 g ai/100 kg seed

Submitter: BASF Crop Protection BASF Crop Protection BASF Crop Protection

MRID Citation: MRID 47470203 MRID 47470203 MRID 47470203

Residues of Triticonazole (ppm)

1

0.009 0.007 0.010

0.009 0.010 0.020

0.030 0.010 0.020

0.030 0.020 0.020

0.020 0.020 0.030

0.020 0.020 0.020

0.020 0.009 0.008

0.020 0.009 0.009

0.020 0.020 0.010

0.020 0.030 0.020

0.050 0.070 0.080

0.060 0.070 0.080

0.090 0.030 0.080

0.080 0.040 0.080

0.020 0.030 0.040

0.020 0.020 0.040

0.030 0.060 0.040

0.030 0.040 0.060

1 Bolded residues are MLE calculated values for residues reported as below the LOQ (<0.01 ppm).
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 35 of 37

Figure I-1. Lognormal probability plot of triticonazole field trial data for wheat forage.

Lognormal Probability Plot

0.1 1 2 5 10 20 30 50 70 80 90 95 98 99 99.9

1.0000

0.5000

0.2500

0.1250

0.0625

0.0313

0.0156

0.0078

0.0039

y = 0.8382x - 3.6367

R

2

 = 0.9514

Percentiles

Concentrations

EPA Triticonazole Wheat, Forage (MLE) Not applicable 50 g ai/100 kg seed BASF Crop

Protection

Figure I-2. Tolerance spreadsheet summary of triticonazole field trial data for wheat forage.

Regulator: EPA

Chemical: Triticonazole

Crop: Wheat, Forage (MLE)

PHI: Not applicable

App. Rate: 50 g ai/100 kg seed

Submitter: BASF Crop Protection

n: 30

min: 0.00

max: 0.10

median: 0.02

average: 0.04

95th Percentile 99th Percentile 99.9th Percentile

0.09 0.15 0.15

(0.10) (0.15) (--)

0.15 0.20 0.35

(0.20) (0.35) (--)

0.15

0.15

0.15

0.9514

p-value > 0.05 : Do not reject lognormality assumption

Approximate 

Shapiro-Francia 

Normality Test 

EU Method I

Normal

UCLMedian95th

95/99 Rule

EU Method II

Distribution-Free

Mean+3SD
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 36 of 37

Figure I-3. Lognormal probability plot of triticonazole field trial data for wheat hay.

Lognormal Probability Plot

0.1 1 2 5 10 20 30 50 70 80 90 95 98 99 99.9

1.0000

0.5000

0.2500

0.1250

0.0625

0.0313

0.0156

0.0078

0.0039

0.0020

y = 1.0293x - 3.866

R

2

 = 0.9405

Percentiles

Concentrations

EPA Triticonazole Wheat, Hay (MLE) Not applicable 50 g ai/100 kg seed BASF Crop

Protection

Figure I-4. Tolerance spreadsheet summary of triticonazole field trial data for wheat hay.

Regulator: EPA

Chemical: Triticonazole

Crop: Wheat, Hay (MLE)

PHI: Not applicable

App. Rate: 50 g ai/100 kg seed

Submitter: BASF Crop Protection

n: 30

min: 0.00

max: 0.08

median: 0.02

average: 0.03

95th Percentile 99th Percentile 99.9th Percentile

0.08 0.10 0.15

(0.10) (0.15) (--)

0.15 0.25 0.60

(0.25) (0.50) (--)

0.15

0.15

0.15

0.9405

p-value > 0.05 : Do not reject lognormality assumption

Approximate 

Shapiro-Francia 

Normality Test 

EU Method I

Normal

UCLMedian95th

95/99 Rule

EU Method II

Distribution-Free

Mean+3SD
Triticonazole Summary of Analytical Chemistry and Residue Data DP#s: 360624; 325956, 353372

Page 37 of 37

Figure I-5. Lognormal probability plot of triticonazole field trial data for wheat straw.

Lognormal Probability Plot

0.1 1 2 5 10 20 30 50 70 80 90 95 98 99 99.9

1.0000

0.5000

0.2500

0.1250

0.0625

0.0313

0.0156

0.0078

0.0039

0.0020

y = 0.8931x - 3.752

R

2

 = 0.9502

Percentiles

Concentrations

EPA Triticonazole Wheat, Straw (MLE) Not applicable 50 g ai/100 kg seed BASF Crop

Protection

Figure I-6. Tolerance spreadsheet summary of triticonazole field trial data for wheat straw.

Regulator: EPA

Chemical: Triticonazole

Crop: Wheat, Straw (MLE)

PHI: Not applicable

App. Rate: 50 g ai/100 kg seed

Submitter: BASF Crop Protection

n: 30

min: 0.00

max: 0.08

median: 0.03

average: 0.03

95th Percentile 99th Percentile 99.9th Percentile

0.08 0.09 0.15

(0.09) (0.15) (--)

0.15 0.20 0.40

(0.20) (0.40) (--)

0.09

0.15

0.15

0.9502

p-value > 0.05 : Do not reject lognormality assumption

Approximate 

Shapiro-Francia 

Normality Test 

EU Method I

Normal

UCLMedian95th

95/99 Rule

EU Method II

Distribution-Free

Mean+3SD