### The following material represents the individualmale reproductivedatafrom the journal article (obtainedvia email communication withDr.Julie Boberg): Boberg, J; Christiansen, S; Axelstad, M; et al. (2010)Reproductive and behavioral effects of diisononylphthalate (DINP) in perinatally exposed rats. Reproductive Toxicology. 2011 Feb; 31(2):200

-9. Epub 2010 Nov 12[HERO ID 806135]Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 1 of 19 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

4 11 1 1 1 5.70 25.00 3.00 13.99528425 4 11 1 2 1 5.90 23.00 2.00 12.7284982 4 11 1 3 1 5.80 20.00 3.00 11.13150787 4 11 1 4 1 5.90 22.00 3.00 12.17508523 4 11 1 5 1 5.90 19.00 4.00 10.51484634 4 11 1 6 1 5.90 24.00 3.00 13.28191116 4 11 1 7 1 6.30 18.00 1.00 9.74598313 4 11 1 8 1 5.20 20.00 0.00 11.54415673 4 11 1 9 2 5.00 10.00 12.00 5.848035476 4 11 1 10 2 5.50 13.00 13.00 7.364712354 4 11 1 11 2 5.40 12.00 12.00 6.839903787 4 11 1 12 2 5.90 12.00 12.00 6.640955582 4 11 1 13 2 6.10 12.00 12.00 6.567568851 4 11 1 14 2 5.50 11.00 12.00 6.231679684 4 11 1 15 2 5.70 13.00 12.00 7.277547808 4 11 1 16 2 5.00 10.00 5.848035476 7 11 2 1 1 6.90 26.00 0.00 13.65705267 7 11 2 2 1 6.80 25.00 2.00 13.1958399 7 11 2 3 1 5.70 23.00 2.00 12.87566151 7 11 2 4 1 6.20 23.00 1.00 12.51979659 7 11 2 5 2 6.20 12.00 12.00 6.532067785 7 11 2 6 2 5.70 12.00 12.00 6.717736438 7 11 2 7 2 4.70 12.00 12.00 7.163885348 7 11 2 8 2 5.90 13.00 12.00 7.194368548 7 11 2 9 2 6.60 14.00 13.00 7.46357195 8 11 2 1 1 6.00 24.00 1.00 13.207709 8 11 2 2 1 7.10 24.00 1.00 12.48700998 8 11 2 3 1 6.40 20.00 1.00 10.77217345 8 11 2 4 1 6.10 22.00 1.00 12.04054289 8 11 2 5 1 6.70 22.00 1.00 11.66982688 8 11 2 6 1 6.20 21.00 2.00 11.43111862 8 11 2 7 2 6.20 12.00 13.00 6.532067785 8 11 2 8 2 6.10 12.00 12.00 6.567568851 8 11 2 9 2 6.80 13.00 12.00 6.861836748 8 11 2 10 2 5.80 12.00 12.00 6.678904724 11 11 3 1 1 6.40 22.00 4.00 11.8493908 11 11 3 2 1 5.90 22.00 4.00 12.17508523Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 2 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

11 11 3 3 1 4.90 21.00 3.00 12.3638559 11 11 3 4 1 5.20 20.00 4.00 11.54415673 11 11 3 5 1 5.80 22.00 4.00 12.24465866 11 11 3 6 1 5.70 23.00 6.00 12.87566151 11 11 3 7 1 5.90 22.00 1.00 12.17508523 11 11 3 8 1 4.60 21.00 1.00 12.62699526 11 11 3 9 1 6.30 22.00 11.91175716 11 11 3 10 2 5.50 11.00 12.00 6.231679684 12 11 3 1 1 5.90 22.00 1.00 12.17508523 12 11 3 2 1 5.10 23.00 2.00 13.36198902 12 11 3 3 1 5.60 22.00 4.00 12.38872668 12 11 3 4 1 5.70 21.00 2.00 11.75603877 12 11 3 5 2 5.40 11.50 12.00 6.554907796 12 11 3 6 2 5.00 12.00 12.00 7.017642572 12 11 3 7 2 5.60 12.00 12.00 6.757487283 12 11 3 8 2 6.20 12.00 12.00 6.532067785 12 11 3 9 2 5.90 12.00 12.00 6.640955582 12 11 3 10 2 5.00 13.00 12.00 7.602446119 12 11 3 11 2 5.90 12.00 12.00 6.640955582 16 11 4 1 1 6.70 22.00 4.00 11.66982688 16 11 4 2 1 6.10 20.00 7.00 10.94594808 16 11 4 3 1 5.70 21.00 5.00 11.75603877 16 11 4 4 1 5.90 21.00 3.00 11.62167227 16 11 4 5 1 5.80 20.00 4.00 11.13150787 16 11 4 6 1 6.50 21.00 3.00 11.25247809 16 11 4 7 2 5.60 11.00 12.00 6.194363342 16 11 4 8 2 5.90 10.00 12.00 5.534129652 16 11 4 9 2 6.00 12.00 12.00 6.603854498 16 11 4 10 2 5.90 10.00 12.00 5.534129652 16 11 4 11 2 5.50 11.00 12.00 6.231679684 16 11 4 12 2 5.80 11.00 12.00 6.12232933 19 11 5 1 1 6.30 20.00 4.00 10.82887014 19 11 5 2 1 6.40 21.00 4.00 11.31078212 19 11 5 3 2 6.00 11.00 12.00 6.05353329 19 11 5 4 2 6.50 11.00 12.00 5.894155188 19 11 5 5 2 5.30 11.00 13.00 6.309099771 19 11 5 6 2 6.40 11.00 12.00 5.924695398 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 3 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

19 11 5 7 2 6.00 10.00 13.00 5.503212081 20 11 5 1 1 5.20 22.00 4.00 12.69857241 20 11 5 2 2 5.60 12.00 12.00 6.757487283 20 11 5 3 2 5.30 11.00 12.00 6.309099771 20 11 5 4 2 5.40 12.00 12.00 6.839903787 21 12 1 1 1 6.30 26.00 5.00 14.07753119 21 12 1 2 1 6.60 26.00 4.00 13.86091934 21 12 1 3 1 6.20 25.00 2.00 13.60847455 21 12 1 4 1 6.30 26.00 2.00 14.07753119 21 12 1 5 1 6.30 21.00 4.00 11.37031365 21 12 1 6 1 6.20 23.00 3.00 12.51979659 21 12 1 7 2 6.20 13.00 12.00 7.076406767 21 12 1 8 2 6.30 14.00 12.00 7.580209101 21 12 1 9 2 6.00 12.00 13.00 6.603854498 21 12 1 10 2 6.10 13.00 13.00 7.114866255 21 12 1 11 2 6.40 13.00 12.00 7.001912743 21 12 1 12 2 6.00 10.00 12.00 5.503212081 21 12 1 13 2 6.00 10.00 13.00 5.503212081 24 12 1 1 1 5.90 24.00 1.00 13.28191116 24 12 1 2 1 6.50 23.00 1.00 12.32414267 24 12 1 3 1 6.20 25.00 0.00 13.60847455 24 12 1 4 1 6.50 23.00 1.00 12.32414267 24 12 1 5 1 6.30 22.00 1.00 11.91175716 24 12 1 6 1 6.10 13.00 12.00 7.114866255 24 12 1 7 2 6.00 13.00 12.00 7.154175706 24 12 1 8 2 5.70 12.00 13.00 6.717736438 24 12 1 9 2 6.20 13.00 12.00 7.076406767 24 12 1 10 2 6.20 11.00 12.00 5.987728803 24 12 1 11 2 5.90 12.00 12.00 6.640955582 24 12 1 12 2 5.60 12.00 12.00 6.757487283 24 12 1 13 2 6.20 12.00 12.00 6.532067785 25 12 2 1 2 5.40 11.00 12.00 6.269911804 25 12 2 2 2 5.60 11.00 12.00 6.194363342 25 12 2 3 2 6.10 12.00 13.00 6.567568851 25 12 2 4 2 6.40 12.00 6.46330407 25 12 2 5dead 26 12 2 1 1 6.50 20.00 2.00 10.7166458 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 4 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

26 12 2 2 1 6.20 22.00 3.00 11.97545761 26 12 2 3 2 5.10 11.00 13.00 6.390516489 26 12 2 4 2 5.90 12.00 12.00 6.640955582 26 12 2 5 2 6.10 13.00 13.00 7.114866255 26 12 2 6 2 5.70 12.00 12.00 6.717736438 26 12 2 7 2 5.60 13.00 13.00 7.320611223 28 12 2 1 1 6.60 22.00 3.00 11.72847021 28 12 2 2 1 6.20 22.00 3.00 11.97545761 28 12 2 3 1 6.40 19.00 3.00 10.23356478 28 12 2 4 2 6.00 10.00 12.00 5.503212081 28 12 2 5 2 5.90 10.00 13.00 5.534129652 28 12 2 6 2 5.70 12.00 12.00 6.717736438 28 12 2 7 2 5.70 11.00 12.00 6.157925068 28 12 2 8 2 5.20 11.00 12.00 6.349286203 28 12 2 9 2 6.00 11.00 13.00 6.05353329 28 12 2 10 2 6.40 10.00 12.00 5.386086725 28 12 2 11 2 5.60 10.00 12.00 5.631239402 29 12 3 1 1 6.00 19.00 2.00 10.45610295 29 12 3 2 1 4.60 19.00 1.00 11.42442428 29 12 3 3 1 6.20 19.00 3.00 10.34244066 29 12 3 4 1 6.30 20.00 1.00 10.82887014 29 12 3 5 1 6.30 20.00 2.00 10.82887014 29 12 3 6 1 5.60 18.00 0.00 10.13623092 29 12 3 7 1 6.40 19.00 2.00 10.23356478 29 12 3 8 1 6.40 20.00 10.77217345 29 12 3 9 1 6.60 19.00 10.12913336 29 12 3 10 2 6.10 11.00 12.00 6.020271446 29 12 3 11 2 5.90 10.00 12.00 5.534129652 29 12 3 12 2 6.00 10.00 13.00 5.503212081 29 12 3 13 2 6.10 11.00 13.00 6.020271446 30 12 3 1 1 6.50 25.00 2.00 13.39580725 30 12 3 2 1 6.90 21.00 4.00 11.03069639 30 12 3 3 1 6.60 25.00 2.00 13.32780705 30 12 3 4 1 7.00 22.00 4.00 11.50067509 30 12 3 5 1 6.40 24.00 3.00 12.92660814 30 12 3 6 1 5.60 24.00 2.00 13.51497457 30 12 3 7 1 6.60 24.00 3.00 12.79469477 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 5 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

30 12 3 8 2 6.50 13.00 12.00 6.965819768 31 12 3 1 1 6.40 22.50 4.00 12.11869513 31 12 3 2 1 6.40 21.00 1.00 11.31078212 31 12 3 3 1 4.90 18.00 3.00 10.59759077 31 12 3 4 1 6.30 24.00 3.00 12.99464417 31 12 3 5 1 6.60 20.00 1.00 10.66224564 31 12 3 6 2 6.10 13.00 12.00 7.114866255 31 12 3 7 2 6.00 11.00 12.00 6.05353329 31 12 3 8 2 5.80 12.00 13.00 6.678904724 31 12 3 9 2 5.30 11.00 12.00 6.309099771 31 12 3 10 2 6.30 10.00 13.00 5.414435072 31 12 3 11 2 6.10 10.00 12.00 5.472974042 32 12 3 1 1 5.90 21.00 4.00 11.62167227 32 12 3 2 1 6.30 24.00 4.00 12.99464417 32 12 3 3 1 6.00 23.00 2.00 12.65738779 32 12 3 4 1 6.50 22.00 4.00 11.78831038 32 12 3 5 1 5.90 22.00 3.00 12.17508523 32 12 3 6 1 6.30 25.00 2.00 13.53608768 32 12 3 7 2 6.40 14.00 13.00 7.540521415 32 12 3 8 2 6.40 14.00 12.00 7.540521415 33 12 4 1 1 5.60 20.00 3.00 11.2624788 33 12 4 2 1 6.00 22.00 4.00 12.10706658 33 12 4 3 1 4.90 20.00 4.00 11.77510086 33 12 4 4 1 5.80 18.00 4.00 10.01835709 33 12 4 5 1 5.80 23.00 5.00 12.80123405 33 12 4 6 1 5.40 20.00 3.00 11.39983964 33 12 4 7 2 5.80 10.00 12.00 5.565753937 33 12 4 8 2 5.60 12.00 13.00 6.757487283 33 12 4 9 2 5.60 10.00 12.00 5.631239402 33 12 4 10 2 5.60 13.00 12.00 7.320611223 33 12 4 11 2 5.70 10.00 13.00 5.598113698 33 12 4 12 2 5.00 10.00 12.00 5.848035476 33 12 4 13 2 5.80 10.00 12.00 5.565753937 35 12 4 1 1 6.10 23.00 1.00 12.5878403 35 12 4 2 1 6.40 22.00 1.00 11.8493908 35 12 4 3 1 6.50 22.00 1.00 11.78831038 35 12 4 4 2 6.20 13.00 12.00 7.076406767 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 6 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

35 12 4 5 2 6.00 12.00 12.00 6.603854498 35 12 4 6 2 6.30 14.00 13.00 7.580209101 36 12 4 1 1 6.70 24.00 3.00 12.73072023 36 12 4 2 1 6.30 23.00 1.00 12.45320067 36 12 4 3 2 6.40 13.00 13.00 7.001912743 36 12 4 4 2 6.30 12.00 12.00 6.497322087 36 12 4 5 2 6.40 12.00 12.00 6.46330407 36 12 4 6 2 6.40 12.00 13.00 6.46330407 36 12 4 7 2 5.10 12.00 12.00 6.971472534 36 12 4 8 1 36 12 4 9 2

37 12 5 1 1 6.00 23.00 3.00 12.65738779 37 12 5 2 1 6.30 20.00 3.00 10.82887014 37 12 5 3 1 5.70 22.00 4.00 12.31585014 37 12 5 4 5.10 13.00 12.00 7.552428578 37 12 5 5 5.10 12.00 12.00 6.971472534 37 12 5 6 6.00 12.00 12.00 6.603854498 37 12 5 7 5.30 10.00 13.00 5.735545246 37 12 5 8 6.30 12.00 13.00 6.497322087 37 12 5 9 5.90 13.00 12.00 7.194368548 37 12 5 10 5.10 11.00 12.00 6.390516489 37 12 5 11 5.90 12.00 13.00 6.640955582 37 12 5 12 6.10 13.00 12.00 7.114866255 37 12 5 13 5.00 11.00 13.00 6.432839024 37 12 5 14 6.20 13.00 12.00 7.076406767 37 12 5 15 4.80 10.00 12.00 5.928155507 38 12 5 1 1 5.60 17.00 2.00 9.573106984 38 12 5 2 1 5.20 18.00 3.00 10.38974106 38 12 5 3 1 5.40 18.00 2.00 10.25985568 38 12 5 4 1 4.90 16.00 2.00 9.420080685 38 12 5 5 1 6.20 18.00 1.00 9.798101678 38 12 5 6 1 6.00 19.00 2.00 10.45610295 38 12 5 7 1 5.10 15.00 8.714340667 38 12 5 8 1 6.00 17.00 9.355460539 38 12 5 9 2 5.80 10.00 12.00 5.565753937 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 7 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

39 12 5 1 1 6.50 21.00 2.00 11.25247809 39 12 5 2 1 6.50 20.00 2.00 10.7166458 39 12 5 3 2 6.10 10.00 12.00 5.472974042 39 12 5 4 2 5.70 10.00 12.00 5.598113698 39 12 5 5 2 5.90 11.00 12.00 6.087542617 39 12 5 6 2 5.40 11.00 12.00 6.269911804 39 12 5 7 2 6.20 11.00 13.00 5.987728803 39 12 5 8 2 5.80 11.00 13.00 6.12232933 39 12 5 9 2 6.00 10.00 12.00 5.503212081 40 12 5 1 1 5.60 19.00 2.00 10.69935486 40 12 5 2 1 5.20 20.00 4.00 11.54415673 40 12 5 3 1 6.10 21.00 2.00 11.49324549 40 12 5 4 1 5.60 19.00 3.00 10.69935486 40 12 5 5 1 5.30 20.00 3.00 11.47109049 40 12 5 6 1 6.00 19.00 4.00 10.45610295 40 12 5 7 2 5.90 10.00 13.00 5.534129652 40 12 5 8 2 5.40 10.00 13.00 5.699919822 40 12 5 9 2 6.00 10.00 12.00 5.503212081 40 12 5 10 2 5.60 10.00 12.00 5.631239402 40 12 5 11 2 5.70 10.00 12.00 5.598113698 40 12 5 12 1 dead 43 21 1 1 2 6.00 9.00 12.00 4.952890873 43 21 1 2 2 5.70 10.00 12.00 5.598113698 43 21 1 3 2 4.90 9.00 12.00 5.298795385 43 21 1 4 2 6.10 10.00 13.00 5.472974042 43 21 1 5 2 6.30 11.00 12.00 5.955878579 43 21 1 6 2 6.20 10.00 12.00 5.443389821 43 21 1 7 2 5.90 10.00 12.00 5.534129652 47 21 2 1 1 6.40 23.00 2.00 12.38799947 47 21 2 2 1 6.20 21.00 3.00 11.43111862 47 21 2 3 1 5.30 21.00 2.00 12.04464502 47 21 2 4 1 6.30 21.00 2.00 11.37031365 47 21 2 5 1 6.40 21.00 2.00 11.31078212 47 21 2 6 1 5.40 21.00 3.00 11.96983163 47 21 2 7 1 6.40 20.00 3.00 10.77217345 47 21 2 8 2 6.00 11.00 12.00 6.05353329 47 21 2 9 2 5.80 11.00 13.00 6.12232933 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 8 of 19 1 A B C D E F G H I J K L M

1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

47 21 2 10 2 6.50 10.00 12.00 5.358322898 47 21 2 11 2 5.90 11.00 12.00 6.087542617 47 21 2 12 2 6.20 12.00 12.00 6.532067785 48 21 2 1 1 6.30 21.00 1.00 11.37031365 48 21 2 2 1 6.30 16.00 2.00 8.663096115 48 21 2 3 1 6.80 22.00 1.00 11.61233911 48 21 2 4 1 5.40 20.00 3.00 11.39983964 48 21 2 5 1 6.50 22.00 2.00 11.78831038 48 21 2 6 2 5.20 11.00 12.00 6.349286203 48 21 2 7 2 5.70 10.00 12.00 5.598113698 48 21 2 8 2 6.20 12.00 6.532067785 55 21 4 1 2 6.70 11.00 12.00 5.834913438 55 21 4 2 2 6.40 12.00 12.00 6.46330407 56 21 4 1 1 5.40 19.00 3.00 10.82984766 56 21 4 2 1 5.20 19.00 2.00 10.9669489 56 21 4 3 1 5.20 18.00 3.00 10.38974106 56 21 4 4 1 5.50 19.00 2.00 10.76381036 56 21 4 5 2 5.30 11.00 12.00 6.309099771 56 21 4 6 2 5.50 10.00 13.00 5.665163349 56 21 4 7 2 5.20 10.00 12.00 5.772078366 56 21 4 8 2 5.20 11.00 13.00 6.349286203 56 21 4 9 2 4.00 9.00 12.00 5.669644725 56 21 4 10 2 5.40 11.00 12.00 6.269911804 56 21 4 11 2 5.20 9.00 5.19487053 59 21 5 1 1 6.70 20.00 3.00 10.60893352 59 21 5 2 1 6.50 18.00 3.00 9.644981217 59 21 5 3 1 6.60 20.00 3.00 10.66224564 59 21 5 4 1 6.30 19.00 4.00 10.28742664 59 21 5 5 1 6.10 18.00 1.00 9.851353276 59 21 5 6 1 6.30 19.00 3.00 10.28742664 59 21 5 7 1 6.60 20.00 1.00 10.66224564 59 21 5 8 2 5.80 10.00 12.00 5.565753937 59 21 5 9 2 6.10 10.00 12.00 5.472974042 59 21 5 10 2 5.60 10.00 13.00 5.631239402 59 21 5 11 2 6.10 11.00 12.00 6.020271446 60 21 5 1 1 5.40 20.00 5.00 11.39983964 60 21 5 2 1 4.10 17.00 4.00 10.62154359 26 26 26 26 26 26 26 26 26 27 27 27 27 27 27 27 27 27 27 28 28 28 28 28 28 28 28 28 28 29 29 29 29 29 29 29 29Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 9 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

60 21 5 3 1 5.80 20.00 3.00 11.13150787 60 21 5 4 1 5.60 18.00 10.13623092 60 21 5 5 2 4.50 10.00 13.00 6.057068643 60 21 5 6 2 5.40 11.00 12.00 6.269911804 60 21 5 7 2 5.70 11.00 13.00 6.157925068 60 21 5 8 2 5.60 10.00 5.631239402 61 22 1 1 1 6.70 21.00 1.00 11.1393802 61 22 1 2 1 6.60 20.00 2.00 10.66224564 61 22 1 3 1 6.80 21.00 3.00 11.08450552 61 22 1 4 1 7.10 20.00 2.00 10.40584165 61 22 1 5 1 6.70 22.00 1.00 11.66982688 61 22 1 6 1 6.30 20.00 2.00 10.82887014 61 22 1 7 2 6.30 11.00 13.00 5.955878579 61 22 1 8 2 5.60 11.00 12.00 6.194363342 61 22 1 9 2 6.20 10.00 12.00 5.443389821 61 22 1 10 2 6.30 11.00 12.00 5.955878579 62 22 1 1 1 6.40 19.00 1.00 10.23356478 62 22 1 2 1 6.20 19.00 3.00 10.34244066 62 22 1 3 1 6.40 20.00 1.00 10.77217345 62 22 1 4 1 5.80 18.00 2.00 10.01835709 62 22 1 5 1 6.50 20.00 1.00 10.7166458 62 22 1 6 1 6.00 20.00 1.00 11.00642416 62 22 1 7 2 6.00 10.00 12.00 5.503212081 62 22 1 8 2 5.70 10.00 12.00 5.598113698 63 22 1 1 1 6.80 20.00 2.00 10.55667192 63 22 1 2 1 7.30 22.00 1.00 11.3409228 63 22 1 3 2 6.40 11.00 13.00 5.924695398 63 22 1 4 2 6.70 11.00 12.00 5.834913438 64 22 1 1 1 6.60 22.00 3.00 11.72847021 64 22 1 2 1 6.30 21.00 2.00 11.37031365 64 22 1 3 1 6.60 22.00 2.00 11.72847021 64 22 1 4 1 5.90 16.00 3.00 8.854607443 64 22 1 5 2 5.60 11.00 13.00 6.194363342 64 22 1 6 2 6.00 11.00 13.00 6.05353329 64 22 1 7 2 5.40 11.00 13.00 6.269911804 64 22 1 8 2 6.00 10.00 12.00 5.503212081 64 22 1 9 2 5.70 11.00 13.00 6.157925068 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 10 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

67 22 2 1 1 6.30 19.00 2.00 10.28742664 67 22 2 2 1 6.40 21.00 1.00 11.31078212 67 22 2 3 1 5.90 20.00 2.00 11.0682593 67 22 2 4 2 5.00 10.00 12.00 5.848035476 67 22 2 5 2 6.40 11.00 12.00 5.924695398 67 22 2 6 2 6.20 11.00 13.00 5.987728803 67 22 2 7 2 5.40 10.00 12.00 5.699919822 67 22 2 8 2 6.00 10.00 13.00 5.503212081 68 22 2 1 1 6.10 21.00 1.00 11.49324549 68 22 2 2 1 6.30 21.00 1.00 11.37031365 68 22 2 3 1 6.50 10.00 2.00 5.358322898 68 22 2 4 1 6.30 21.00 3.00 11.37031365 68 22 2 5 1 6.20 22.00 4.00 11.97545761 68 22 2 6 2 5.90 10.00 12.00 5.534129652 68 22 2 7 2 6.10 10.00 12.00 5.472974042 68 22 2 8 2 6.20 11.00 12.00 5.987728803 69 22 3 1 1 6.30 17.00 4.00 9.204539623 69 22 3 2 1 6.00 16.00 4.00 8.80513933 69 22 3 3 1 6.50 18.00 4.00 9.644981217 69 22 3 4 2 6.40 9.00 13.00 4.847478053 69 22 3 5 2 6.30 10.00 12.00 5.414435072 69 22 3 6 2 5.80 9.00 12.00 5.009178543 69 22 3 7 2 6.60 10.00 12.00 5.331122821 69 22 3 8 2 6.10 10.00 13.00 5.472974042 69 22 3 9 2 6.00 10.00 13.00 5.503212081 69 22 3 10 2 6.00 11.00 12.00 6.05353329 70 22 3 1 1 6.20 17.00 2.00 9.253762696 70 22 3 2 1 6.10 19.00 4.00 10.39865068 70 22 3 3 2 6.20 10.00 13.00 5.443389821 70 22 3 4 2 6.10 11.00 12.00 6.020271446 70 22 3 5 2 6.20 10.00 13.00 5.443389821 71 22 3 1 1 6.50 18.00 2.00 9.644981217 71 22 3 2 1 6.30 20.00 4.00 10.82887014 71 22 3 3 1 6.70 20.00 4.00 10.60893352 71 22 3 4 1 6.60 21.00 4.00 11.19535792 71 22 3 5 2 6.40 11.00 12.00 5.924695398 71 22 3 6 2 5.20 9.00 13.00 5.19487053 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 11 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

71 22 3 7 2 5.90 10.00 12.00 5.534129652 71 22 3 8 2 6.40 11.00 13.00 5.924695398 71 22 3 9 2 5.90 10.00 12.00 5.534129652 71 22 3 10 2 5.70 11.00 12.00 6.157925068 71 22 3 11 2 6.10 11.00 13.00 6.020271446 71 22 3 12 2 6.00 11.00 12.00 6.05353329 72 22 3 1 1 6.10 22.00 2.00 12.04054289 72 22 3 2 1 6.40 22.00 4.00 11.8493908 72 22 3 3 1 6.10 20.00 4.00 10.94594808 72 22 3 4 1 6.40 21.00 3.00 11.31078212 72 22 3 5 1 6.20 22.00 2.00 11.97545761 72 22 3 6 2 6.30 12.00 13.00 6.497322087 72 22 3 7 2 5.30 11.00 12.00 6.309099771 72 22 3 8 2 5.90 11.00 12.00 6.087542617 72 22 3 9 2 5.90 11.00 12.00 6.087542617 72 22 3 10 2 5.90 11.00 12.00 6.087542617 72 22 3 11 2 5.90 12.00 12.00 6.640955582 73 22 4 1 1 6.40 18.00 4.00 9.694956105 73 22 4 2 1 6.20 18.00 4.00 9.798101678 73 22 4 3 1 6.60 22.00 3.00 11.72847021 73 22 4 4 1 6.80 21.00 2.00 11.08450552 73 22 4 5 2 5.90 12.00 12.00 6.640955582 73 22 4 6 2 5.80 11.00 12.00 6.12232933 73 22 4 7 2 5.90 10.00 13.00 5.534129652 73 22 4 8 2 5.70 11.00 12.00 6.157925068 73 22 4 9 2 5.40 10.00 12.00 5.699919822 73 22 4 10 2 5.90 11.00 13.00 6.087542617 73 22 4 11 2 5.90 12.00 12.00 6.640955582 74 22 4 1 1 5.80 19.00 4.00 10.57493248 74 22 4 2 1 6.30 20.00 1.00 10.82887014 74 22 4 3 1 5.90 21.00 2.00 11.62167227 74 22 4 4 1 5.80 19.00 4.00 10.57493248 74 22 4 5 1 5.80 20.00 3.00 11.13150787 74 22 4 6 1 6.50 19.00 8.00 10.18081351 74 22 4 7 2 5.60 11.00 12.00 6.194363342 74 22 4 8 2 5.80 12.00 13.00 6.678904724 74 22 4 9 2 6.10 11.00 12.00 6.020271446 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 12 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

74 22 4 10 2 5.60 12.00 12.00 6.757487283 74 22 4 11 2 5.70 11.00 12.00 6.157925068 74 22 4 12 2 5.40 10.00 12.00 5.699919822 74 22 4 13 2 5.70 10.50 13.00 5.878019383 76 22 4 1 1 6.80 18.00 5.00 9.501004728 76 22 4 2 1 6.90 19.00 5.00 9.980153876 76 22 4 3 1 6.40 21.00 2.00 11.31078212 76 22 4 4 1 6.90 22.00 6.00 11.55596765 76 22 4 5 2 6.10 12.00 12.00 6.567568851 76 22 4 6 2 5.60 11.00 12.00 6.194363342 76 22 4 7 2 6.30 11.00 12.00 5.955878579 76 22 4 8 2 6.40 10.00 12.00 5.386086725 76 22 4 9 2 6.20 11.00 13.00 5.987728803 78 22 5 1 1 6.10 17.00 4.00 9.304055872 78 22 5 2 1 6.80 19.00 3.00 10.02883832 78 22 5 3 1 6.30 18.00 3.00 9.74598313 78 22 5 4 1 6.70 22.00 1.00 11.66982688 78 22 5 5 2 6.20 11.00 13.00 5.987728803 78 22 5 6 2 6.60 12.00 12.00 6.397347386 78 22 5 7 2 5.70 9.00 13.00 5.038302328 78 22 5 8 2 6.20 10.00 14.00 5.443389821 78 22 5 9 2 6.30 10.00 12.00 5.414435072 78 22 5 10 2 6.10 12.00 13.00 6.567568851 78 22 5 11 2 6.40 11.00 12.00 5.924695398 80 22 5 1 1 6.60 18.00 4.00 9.596021078 80 22 5 2 1 6.80 20.00 4.00 10.55667192 80 22 5 3 1 6.40 20.00 5.00 10.77217345 80 22 5 4 1 6.30 21.00 5.00 11.37031365

Litter means

4 11 1 8.5 1.50 5.83 21.38 5.51 11.63 2.38 12.14 11.89 6.58 7 11 2 5 1.56 6.40 24.25 5.82 12.60 1.25 12.20 13.06 7.01 8 11 2 5.5 1.40 6.42 22.17 6.23 12.25 1.17 12.25 11.93 6.66 11 11 3 5.5 1.10 5.63 21.67 5.50 11.00 3.38 12.00 12.20 6.23 12 11 3 6 1.64 5.58 22.00 5.57 12.07 2.25 12.00 12.42 6.82 16 11 4 6.5 1.50 6.12 20.83 5.78 10.83 4.33 12.00 11.40 6.04 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439
440 441 442 443 444 445Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 13 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

19 11 5 4 1.71 6.35 20.50 6.04 10.80 4.00 12.40 11.07 5.94 20 11 5 2.5 1.75 5.20 22.00 5.43 11.67 4.00 12.00 12.70 6.64 21 12 1 7 1.54 6.32 24.50 6.14 12.14 3.33 12.43 13.25 6.63 24 12 1 7 1.54 6.28 23.40 5.99 12.25 0.80 12.13 12.69 6.75 25 12 2 3 2.00 5.88 11.50 12.33 6.37 26 12 2 4 1.71 6.35 21.00 5.68 12.20 2.50 12.60 11.35 6.84 28 12 2 6 1.73 6.40 21.00 5.81 10.63 3.00 12.25 11.31 5.92 29 12 3 7 1.31 6.04 19.22 6.03 10.50 1.57 12.50 10.57 5.77 30 12 3 4.5 1.13 6.51 23.57 6.50 13.00 2.86 12.00 12.64 6.97 31 12 3 6 1.55 6.12 21.10 5.93 11.17 2.40 12.33 11.54 6.17 32 12 3 4.5 1.25 6.15 22.83 6.40 14.00 3.17 12.50 12.46 7.54 33 12 4 7 1.54 5.58 20.50 5.59 10.71 3.83 12.29 11.56 6.04 35 12 4 3.5 1.50 6.33 22.33 6.17 13.00 1.00 12.33 12.08 7.09 36 12 4 5 1.67 6.50 23.50 6.12 12.20 2.00 12.40 12.59 6.68 37 12 5 8 1.00 6.00 21.67 5.57 11.83 3.33 12.33 11.93 6.68 38 12 5 5 1.11 5.55 17.25 5.80 10.00 2.00 12.00 9.75 5.57 39 12 5 5 1.78 6.50 20.50 5.87 10.57 2.00 12.29 10.98 5.86 40 12 5 6.5 1.42 5.63 19.67 5.72 10.00 3.00 12.40 11.06 5.59 43 21 1 4 2.00 5.87 9.86 12.14 5.47 47 21 2 6.5 1.42 6.06 21.14 6.08 11.00 2.43 12.20 11.61 6.03 48 21 2 4.5 1.38 6.26 20.20 5.70 11.00 1.80 12.00 10.97 6.16 55 21 4 1.5 2.00 6.55 11.50 12 6.149108754 56 21 4 6 1.64 5.33 18.75 5.11 10.14 2.50 12.33 10.74 5.89 59 21 5 6 1.36 6.44 19.14 5.90 10.25 2.57 12.25 10.29 5.67 60 21 5 4.5 1.50 5.23 18.75 5.30 10.50 4.00 12.67 10.82 6.03 61 22 1 5.5 1.40 6.70 20.67 6.10 10.75 1.83 12.25 10.97 5.89 62 22 1 4.5 1.25 6.22 19.33 5.85 10.00 1.50 12.00 10.51 5.55 63 22 1 2.5 1.50 7.05 21.00 6.55 11.00 1.50 12.50 10.95 5.88 64 22 1 5 1.56 6.35 20.25 5.74 10.80 2.50 12.80 10.92 6.04 67 22 2 4.5 1.63 6.20 20.00 5.80 10.40 1.67 12.40 10.89 5.79 68 22 2 4.5 1.38 6.28 19.00 6.07 10.33 2.20 12.00 10.31 5.66 69 22 3 5.5 1.70 6.27 17.00 6.17 9.86 4.00 12.43 9.22 5.38 70 22 3 3 1.60 6.15 18.00 6.17 10.33 3.00 12.67 9.83 5.64 71 22 3 6.5 1.67 6.53 19.75 5.95 10.50 3.50 12.38 10.57 5.79 72 22 3 6 1.55 6.24 21.40 5.87 11.33 3.00 12.17 11.62 6.29 73 22 4 6 1.64 6.50 19.75 5.79 11.00 3.25 12.29 10.58 6.13 74 22 4 7 1.54 6.02 19.67 5.70 11.07 3.67 12.29 10.82 6.20 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482Birth and lactation data - Individual data from Boberg et al 2010 study (HERO ID 806135) Group 1 = control; group 2 = DINP-300; group 3 = DINP-600; group 4 = DINP-750; group 5 = DINP-900 Dams dosed by gavage

page 14 of 19 1 A B C D E F G H I J K L M

LitterBlockGroup Pup NumberSex Birth weight male AG maleBirth weight female AG femaleNipples maleNipples female AGI male AGI female

76 22 4 5 1.56 6.75 20.00 6.12 11.00 4.50 12.20 10.59 6.02 78 22 5 6.50 1.70 6.60 19.67 6.21 10.71 2.33 12.71 10.48 5.82 80 22 5 2.5 1.00 6.53 19.75 4.50 AG = Anogenital distance measured in arbitrary units AGI = Anogenital index, AG divided by cube root of the body weight

Group means based on litter means 1.00 21.50 5.97 11.05 1.98 12.30 11.60 6.10 2.00 6.30 21.09 5.90 11.32 2.00 12.25 11.43 6.27 Control 3.00 6.12 20.65 6.01 11.38 2.91 12.30 11.31 6.26 DINP-300 4.00 6.14 20.67 5.88 11.27 3.14 12.24 11.29 6.25 DINP-600 5.00 6.00 19.89 5.76 10.70 3.17 12.34 11.01 5.98 DINP-750 DINP-900 0.39 1.82 0.31 0.90 0.83 0.26 1.04 0.50 Standard deviation 0.12 1.58 0.19 0.85 0.64 0.19 0.82 0.48 Control 0.32 2.11 0.32 1.30 0.69 0.24 1.20 0.67 DINP-300 0.49 1.55 0.41 0.86 1.21 0.14 0.75 0.38 DINP-600 0.56 1.39 0.29 0.66 0.92 0.25 0.88 0.41 DINP-750 DINP-900 483 484 485 486 487 488

489 490 491 492 493 494 495 496

497 498 499 500 501 502Motility Individual data from Boberg et al 2010 study (HERO ID 806135)

page 15 of 19 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 A B C D E F G H I J K L

Animal number Group Litter Motile sperm (%) Progressive sperm

(%) AP vel. SL vel.CL vel. STR Sperm/gCauda (g) Sperm count

201 Control 4 58 28 157.8 92.3 298.6 58 374.8 0.2182 81.78136 202 Control 4 53 27 141.1 83.4 278.3 58 315.4 0.2403 75.79062 211 Control 24 61 31 170 107.5 342.3 62 518.2 0.242 75.0926 212 Control 24 54 27 165.5 100.4 333.5 59 379.6 0.1978 94.7462 221 Control 21 55 22 177.1 105.7 324.9 58 504.9 0.2118 109.75476 222 Control 21 59 25 163.2 85.2 334 56 310.3 0.2475 93.951 223 Control 21 40 25 161.9 98.9 327.1 61 479 0.2476 125.01324 224 Control 24 62 34 164.8 98.7 343.8 61 367.5 0.234 85.995 251 Control 61 70 43 169.8 101.7 355.9 61 389.5 0.2136 83.1972 252 Control 61 63 43 158.6 98.1 325 62 483.6 0.28 135.408 257 Control 61 67 45 178 114.8 353.7 65 334.2 0.2467 82.44714 258 Control 62 64 37 166.2 104 331.1 63 506 0.2287 115.7222 265 Control 64 67 34 171.5 107.2 333.6 62 381.4 0.2292 108.6408 266 Control 64 61 31 168.4 96.7 366 58 377.1 0.2035 134.2286 275 Control 62 58 29 158 93.9 316.2 60 474 0.2192 83.60288 276 Control 62 56 34 168.7 100.4 358.1 61 659.6 0.2123 80.05833 203 DINP 300 7 41 21 151 84.5 309.5 57 338.9 0.2214 75.03246 204 DINP 300 7 61 36 169.6 104.7 329 61 531.5 0.204 108.426 213 DINP 300 8 59 31 138.6 81.3 302 60 402.5 0.2411 97.04275 214 DINP 300 8 53 29 166.4 104.8 338.1 62 527.1 0.2223 117.17433 225 DINP 300 8 48 32 143.9 87.8 317 63 550.9 0.1929 106.26861 226 DINP 300 28 52 28 167.6 98.9 343 58 513.1 0.217 111.3427 241 DINP 300 47 62 39 175.1 113.3 347.4 63 466.6 0.21 97.986 242 DINP 300 47 65 35 173.8 104.1 341.3 59 447.5 0.2005 89.72375 245 DINP 300 47 65 38 166.4 103.7 331.9 62 481.5 0.2143 103.18545 246 DINP 300 48 59 32 187.5 112.7 378.6 61 484 0.2235 108.174 249 DINP 300 48 61 35 172.1 107.6 352.2 63 493.8 0.2551 125.96838 250 DINP 300 48 58 36 170.2 104.9 353 62 492.7 0.2331 114.84837 253 DINP 300 67 65 39 139.2 81.8 296.7 60 538.9 0.2262 121.89918 254 DINP 300 68 65 41 156.9 96.9 332.9 63 424 0.1815 76.956 259 DINP 300 68 54 34 174.8 107.2 367.3 62 379.4 0.1749 66.35706 260 DINP 300 68 47 31 161.5 99.7 351.1 61 524.1 0.1868 97.90188 205 DINP 600 11 66 37 192.8 115.7 382.3 59 486.9 0.2401 116.90469 206 DINP 600 11 54 28 168.9 104.9 353.8 62 396.1 0.2308 91.41988 215 DINP 600 12 46 24 154.2 95.6 301.8 63 366.7 0.2169 79.53723 216 DINP 600 12 52 20 170.3 87.2 338.2 53 466.7 0.1779 83.02593Motility Individual data from Boberg et al 2010 study (HERO ID 806135)

page 16 of 19 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 A B C D E F G H I J K L 227 DINP 600 29 43 14 161.5 91 322.8 54 531.6 0.2253 119.76948 228 DINP 600 29 46 26 156.2 96.6 313.7 60 574.4 0.2431 139.63664 231 DINP 600 30 53 28 162.8 108.3 310.3 67 289 0.2691 77.7699 232 DINP 600 30 66 35 166.7 99.4 317.9 61 500.8 0.2224 111.37792 235 DINP 600 31 33 14 158.9 86.8 303.1 58 535.4 0.2134 114.25436 236 DINP 600 31 49 24 144.3 85.9 302.3 60 362.9 0.2213 80.30977 239 DINP 600 32 43 27 153.4 92.4 327.7 62 403.7 0.2122 85.66514 240 DINP 600 32 55 38 156.1 94.4 349.3 63 464.8 0.21 97.608 267 DINP 600 69 55 41 171.5 112.6 362.7 66 461.4 0.2486 114.70404 268 DINP 600 70 51 27 151.7 89.2 317.7 59 431.9 0.2489 107.49991 271 DINP 600 71 53 33 173.4 108 333.7 61 547.9 0.1877 102.84083 272 DINP 600 72 55 29 170.1 102.5 355.5 60 473.4 0.2351 111.29634 207 DINP 750 16 54 29 154.4 98.9 312.7 64 358.8 0.2384 85.53792 208 DINP 750 16 47 18 156 84.2 327.7 55 486.1 0.2263 110.00443 217 DINP 750 35 47 20 160.1 91.7 332.6 58 465 0.2147 92.9651 218 DINP 750 33 47 21 162.5 92.9 331.5 57 433.5 0.2341 108.8565 219 DINP 750 33 50 26 159.3 96.4 326.6 61 306.7 0.1868 80.9778 220 DINP 750 33 41 21 150.9 87.2 306.2 59 433 0.22 67.474 255 DINP 750 56 11 6 148.8 110.1 314.5 71 181.5 0.1216 22.0704 256 DINP 750 56 55 29 173.3 112.6 335.9 64 508.8 0.2184 111.12192 261 DINP 750 73 63 34 146.2 89.2 305.9 61 510 0.2244 114.444 262 DINP 750 73 43 20 145.3 89.4 307.4 61 387.2 0.2377 92.03744 269 DINP 750 74 60 34 163.6 99.8 331.3 59 529.5 0.1979 104.78805 270 DINP 750 74 54 31 161.4 92.2 350 57 441.4 0.2467 108.89338 273 DINP 750 76 51 32 145.4 89.5 324.6 62 463.2 0.2001 92.68632 209 DINP 900 19 37 19 153 81.6 335.5 56 478.8 0.199 95.2812 210 DINP 900 37 46 27 182.1 118.2 380.2 65 507.9 0.2008 101.98632 229 DINP 900 38 58 38 144.6 86.9 293.4 63 516.2 0.2131 110.00222 230 DINP 900 38 53 21 140.2 71.8 300.7 54 508.3 0.2298 116.80734 233 DINP 900 38 38 24 135.9 78.7 302.8 60 441.3 0.2244 99.02772 234 DINP 900 40 50 23 169 107 347.6 60 501.7 0.2042 102.44714 237 DINP 900 40 62 31 162.7 99.7 326 60 552.5 0.2352 129.948 238 DINP 900 40 43 19 150.7 80.3 310.4 55 505.1 0.1823 92.07973 243 DINP 900 59 58 35 162.7 102 339.1 64 661.1 0.2238 147.95418 244 DINP 900 59 43 27 164.2 98.4 367.4 61 491.3 0.2082 102.28866 247 DINP 900 59 60 33 160.2 94.5 331.6 59 346 0.2151 74.4246 248 DINP 900 60 43 25 181.1 104.9 382.8 59 500 0.2147 107.35 263 DINP 900 78 48 26 160 94.1 342.5 59 443.5 0.2325 103.11375 264 DINP 900 78 53 31 169.8 98.9 362.1 59 537 0.2157 115.8309Motility Individual data from Boberg et al 2010 study (HERO ID 806135)

page 17 of 19 77 78 79

80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97

98 99 100 101 102 103 104 105 106

107 108 109 A B C D E F G H I J K L Animal 255: Both left and right epididymis and testes are very small. Very few motile sperm.

Group mean values motile sperm

(%) progressive sperm

(%) average path velocity straight line velocity curvilinear velocitystraightness sperm/ gram cauda sperm count

Control 59.25 32.19 165.04 99.31 332.63 60.31 428.44 0.23 97.84 DINP 300 57.19 33.56 163.41 99.62 336.94 61.06 474.78 0.21 101.14 DINP 600 51.25 27.81 163.30 98.16 330.80 60.50 455.85 0.23 102.10 DINP 750 47.92 24.69 155.94 94.93 323.61 60.69 423.44 0.21 91.68 DINP 900 49.43 27.07 159.73 94.07 337.29 59.57 499.34 0.21 107.04

Standard deviation Control 7.09 6.94 8.79 8.08 22.36 2.33 93.80 0.02 20.73 DINP 300 7.34 5.02 13.98 10.41 22.52 1.84 61.51 0.02 17.08 DINP 600 8.25 7.82 11.49 9.55 24.16 3.65 77.20 0.02 17.87 DINP 750 12.74 8.01 8.46 8.53 13.41 4.11 96.36 0.03 25.14 DINP 900 8.16 5.86 13.83 12.81 28.97 3.16 68.71 0.01 17.44

Litter has been included in the statistical analysis as a random factor in the ANOVA test Statistical analysis has been performed both with and without animal number 255

CONTROL GROUP

Litter label

number Litter Mean Mean of litters Standard Deviation of litters 4 55.50 59.31 5.56 24 59.00 21 51.33 61 66.67 62 59.33 64 64.00

DINP 300

Litter label

number Litter Mean Mean of litters Standard deviation of litters 7 51.00 56.78 6.16 8 53.33Motility Individual data from Boberg et al 2010 study (HERO ID 806135)

page 18 of 19 110 1 1 21 31 41 51

61 7128 52.00 47 64.00 67 65.00 68 55.33

DINP 600

Litter label

number Litter Mean Mean of litters Standard Deviation of litters 11 60.00 51.70 6.10 12 49.00 29 44.50 30 59.50 31 41.00 32 49.00 69 55 70 51 71 53 72 55

DINP 750

Litter label

number Litter Mean Mean of litters Standard Deviation of litters 16 50.50 48.21 7.65 35 47.00 33 46.00 56 33.00 73 53.00 74 57.00 76 51

or without animal 255 in litter labeled 56 Animal 255: Both left and right epididymis and testes are very small. Very few motile sperm. 16 50.50 51.36 4.01 35 47.00 33 46.00 56 55.00 73 53.00 81 91 02 12 22 32 42 52 62 72 82

92 03 13 23 33 43 53 63 73 83 93 04 14 24 34 44 1 1 1 1 1

1 1 1 1 1 1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 A B C D E F G H I J K LMotility Individual data from Boberg et al 2010 study (HERO ID 806135)

page 19 of 19 145 146 147 148

149 150 151 152 153 154 155 A B C D E F G H I J K L 74 57.00 76 51

DINP 900

Litter label

number Litter Mean Mean of litters Standard Deviation of litters 19 37.00 46.31 5.57 37 46.00 38 49.67 40 51.67 60 43.00 78 50.5

### Testis histology data from Boberg et al 2010 study (HERO ID 806135)

Dose groupLitter numberpup number MNG GC changechord diameter increase
1 1 e 0 0 0 1 1 h 0 0 0 1 41 f 0 0 0 1 42 j 0 0 0 1 42 f 0 0 0 1 42 k 0 0 0 1 42 h 0 0 0 2 5 h 1 0 0 2 6 e 0 0 0 2 6 k 0 0 0 2 6 j 0 0 0 2 6 h 0 0 0 2 45 g 0 0 0 2 45 d 1 0 0 2 46 g 0 0 0 3 9 f 1 0 0 3 9 c 0 0 0 3 10 h 1 0 0 3 10 e 0 1 1 3 52 f 1 1 1 4 14 i 1 1 0 4 15 l 1 1 0 4 15 h 0 0 0 4 53 j 1 1 1 4 53 p 1 1 1 4 53 e 1 1 1 4 53 i 1 1 1 5 17 g 1 1 1 5 17 i 1 1 1 5 17 c 1 0 0 5 17 e 1 1 0 5 18 d 1 0 0 5 58 c 1 1 1

total number single animals of pups MNG GC changechord diameter increase
1 7 0 0 0 2 8 2 0 0 3 5 3 2 2 4 7 6 6 4 5 6 6 4 3

litters total number ofMNG GC changechord diameter increase
1 3 0 0 0 2 4 2 0 0 3 3 3 2 2 4 3 3 3 1 5 3 3 2 2

### Testosterone production data from Boberg et al 2010 study (HERO ID 806135)

Dose groupLitter numberPup numberTesticular testosterone production
1 41 e 1.16 1 41 c 2.16 1 42 c 0.58 1 42 e 0.82 1 1 b 1.16 1 1 c 9.16 2 45 a 0.94 2 45 b 2.30 2 46 d 1.25 2 46 e 1.51 2 5 a 0.75 2 5 d 0.52 2 6 b 1.67 2 6 d 0.79 3 51 a 0.44 3 51 b 0.21 3 52 c 0.33 3 52 e 0.73 3 9 a 0.66 3 9 b 0.43 3 10 b 1.79 3 10 d 0.55 4 53 a 0.06 4 53 b 0.15 4 54 f 2.10 4 54 c 0.59 4 14 a 1.38 4 14 g 1.11 4 15 b 0.46 4 15 c 0.43 5 57 b 0.31 5 57 d 0.05 5 58 a 1.94 5 58 b 0.16 5 17 a 0.36 5 17 b 0.23 5 18 a 0.86 5 18 b Control litter label #Litter Meanmean of littersSD of litters 41.00 1.66 2.51 2.35 42.00 0.70

1.00 5.16

DINP 300 litter label #Litter Meanmean of littersSD of litters
45 1.62 1.22 0.42 46 1.38

5 0.64 6 1.23

DINP 60051 0.32 0.64 0.37 52 0.53

9 0.54 10 1.17

DINP 750

53 0.11 0.78 0.61 54 1.35 14 1.25 15 0.44

DINP 900 litter label #Litter Meanmean of littersSD of litters
57 0.18 0.60 0.42 58 1.05 17 0.30 18 0.86

### Testosterone content data from Boberg et al 2010 study (HERO ID 806135)

Dose groupLitter numberanimal numbernM ng per testis 1 1 e 80.1 11.5344 1 1 f 59.4 8.5536 1 41 k 46.9 6.7536 1 41 f 81 11.664 1 42 g 37.5 5.4 1 42 f 61.2 8.8128 2 5 h 53.7 7.7328 2 6 e 36.7 5.2848 2 6 g 52.5 7.56 2 45 e 43 6.192 2 45 d 66.6 9.5904 2 46 g 58.4 8.4096 3 9 c 39.9 5.7456 3 9 e 45.7 6.5808 3 10 e 38.5 5.544 3 10 g 45.1 6.4944 3 51 c 31.2 4.4928 3 52 f 5.8 0.83952 4 14 i 46.8 6.7392 4 15 h 67.7 9.7488 4 15 j 49.4 7.1136 4 53 d 18.1 2.6064 4 53 c 25.9 3.7296 5 17 c 35.5 5.112 5 17 h 33.4 4.8096 5 18 d 28.5 4.104 5 18 e 52.2 7.5168 5 58 d 20.2 2.9088 5 58 c 58.4 8.4096