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2011_March _Progress Report 1

REVISED - PROGRESS REPORT: MARCH 2011 

BIODEGRADATION TESTING SUPPLEMENTAL ENVIRONMENTAL PROJECT 

Submitted to 

E.I du Pont de Nemours and Company 

Wilmington, Delaware 19898 

U.S.A. 

United States Environmental Protection Agency 

Washington, DC 

U.S.A. 

Wildlife International, Ltd.

8598 Commerce Drive 

Easton, Maryland 21601 USA 

1 (410) 822-8600 

March 29, 2011 

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Table of Contents 

Title Page ...................................................................................................................................1 

Table of Contents.......................................................................................................................2 

Introduction................................................................................................................................3 

Progress Summary .....................................................................................................................3 

Appendix A. Invoice.................................................................................................................4 

Appendix B. Revised Certificate of Analysis for M+2 8-2 FTUCA Analytical Standard .......6 
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2011_March _Progress Report 3 

Introduction 

This document presents the progress of the technical work for the Biodegradation Testing 

Supplemental Environmental Project (“Biodegradation SEP”) performed from 26 February 

2011 up to 27 March 2011. Studies conducted under protocol will be examined by the 

Wildlife International Quality Assurance Unit for compliance with Good Laboratory 

Practices, Standard Operating Procedures and the specified protocol. Raw data that has been 

audited by the Wildlife International Quality Assurance will be included in the progress 

report, as appropriate. 

Progress Summary

The total costs associated with the project for the period covered in this Progress Report are 

approximately $8,075 for labor and $18,900 for instrumentation. The invoice and an 

allocation of costs with associated tasks are presented in Appendix A. 

Work conducted over the period covered by this progress report consisted of review of data 

from the initial pilot study, discussion of Task E items with stakeholders and preparations for 

the next method refinement trial. 

An updated certificate of analysis was received from Wellington Laboratories for one of the 

mass-labeled acid analyte standard materials (M+2 8-2 FTUA) that had been originally 

received in November 2009. The new certificate of analysis is presented in Appendix B. 
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Appendix A 

Invoice 
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2011_March _Progress Report 

Allocation of Costs 

Month, Year Tasks 

 Design and 

Procurement of 

SCAS Units 

(Task A) 

Acquisition 

of Standards 

(Task B) 

Adaptation of 

Analytical 

Method (Task C) 

Pilot Studies 

Task (D) 

Refinement of 

Methods 

Task (E) 

Method 

Validation

s (Task F) 

Storage 

Stability 

(Task G) 

Biod

dat

Stu

(Tas

Dec 2009 $1,803 $1,085 $42,422 Jan 2010 $29,227 Feb 2010 $1,300 $36,745 Mar 2010 $14,350 Apr 2010 $570 $15,935 May 2010 $760 $71,860 June 2010 $57,870 $35,003 July 2010 $42,430 $15,130 August 2010 $32,530 $113,718 September 2010 $1,350 $115,228 October 2010 $88,823 $3,613 November 2010 $42,690 $855 $570 December 2010 $18,385 $3,530 $3040 January 2011 $15,570 February 2011 $2,945 March 2011 $8,075 Labor Total $1,803 $3,715 $344,719 $428,988 $34,768 $3,610 Materials $6,800 $10,500 $20,644 $1,724 Total Cost $8,603 $14,215 $365,363 $430,712 $34,768 $3,610 
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Appendix B 

Revised Certificate of Analysis for M+2 8-2 FTUCA Analytical Standard 
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