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2011_March _Progress Report 1 REVISED - PROGRESS REPORT: MARCH 2011

BIODEGRADATION TESTING SUPPLEMEN TAL ENVIRONMENTAL PROJECT

Submitted to

E.I du Pont de Nemours and Company

Wilmington, Delaware 19898

U.S.A.

United States Environmental Protection Agency

Washington, DC

U.S.A.



# Wildlife International, Ltd.

8598 Commerce Drive

Easton, Maryland 21601 USA

1 (410) 822-8600

March 29, 2011

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2011_March _Progress Report 2 Table of Contents

Title Page ..................................................................................................................... ..............1

Table of Contents.............................................................................................................. .........2

Introduction................................................................................................................... .............3

Progress Summary ............................................................................................................... ......3

Appendix A. Invoice........................................................................................................... ......4

Appendix B. Revised Certificate of Analys is for M+2 8-2 FTUCA Analytical Standard .......6Does Not Contain Confidential Business Information (CBI)

2011_March _Progress Report 3 Introduction

This document presents the pr ogress of the technical work for the Biodegradation Testing

Supplemental Environmental Pr oject (“Biodegradation SEP”) performed from 26 February

2011 up to 27 March 2011. Studies conducted under protocol will be examined by the

Wildlife International Quality Assurance Unit for compliance with Good Laboratory

Practices, Standard Operating Procedures and the specified protocol. Raw data that has been

audited by the Wildlife International Quality Assurance will be included in the progress

report, as appropriate.

Progress Summary

The total costs associated with the project for the period covered in this Progress Report are

approximately $8,075 for labor and $18,900 for in strumentation. The invoice and an

allocation of costs with associated tasks are presented in Appendix A.

Work conducted over the period co vered by this progress report c onsisted of review of data

from the initial pilot study, discussion of Task E items with stakeholders and preparations for

the next method refinement trial.

An updated certificate of analysis was received from Wellington Laboratories for one of the

mass-labeled acid analyte standard materials (M+2 8-2 FTUA) that had been originally

received in November 2009. The new certificate of analysis is presented in Appendix B.Does Not Contain Confidential Business Information (CBI)

2011_March _Progress Report 4 Appendix A

InvoiceDoes Not Contain Confidential Business Information (CBI)2011_March _Progress Report5Allocation of CostsMonth, Year Tasks TotalDesign andProcurement ofSCAS Units(Task A)Acquisitionof Standards(Task B)Adaptation ofAnalyticalMethod (Task C)Pilot StudiesTask (D)Refinement ofMethodsTask (E)MethodValidations (Task F)StorageStability(Task G)BiodegradationStudies(Task H)InstrumentLeaseDec 2009 $1,803 $1,085 $42,422 $45,310Jan 2010 $29,227 $29,227Feb 2010 $1,300 $36,745 $38,045Mar 2010 $14,350 $14,350 $18,900Apr 2010 $570 $15,935 $16,505 $18,900May 2010 $760 $71,860 $72,620 $18,900June 2010 $57,870 $35,003 $92,873 $18,900July 2010 $42,430 $15,130 $57,560 $18,900August 2010 $32,530 $113,718 $146,248 $18,900September 2010 $1,350 $115,228 $116,578 $18,900October 2010 $88,823 $3,613 $92,478 $18,900November 2010 $42,690 $855 $570 $44,115 $18,900December 2010 $18,385 $3,530$3040 $24,955 $18,900January 2011 $15,570 $15,570 $18,900February 2011 $2,945 $2,945 $18,900March 2011 $8,075 $8,075 $18,900Labor Total $1,803 $3,715 $344,719 $428,988 $34,768 $3,610 $817,602Materials $6,800 $10,500 $20,644 $1,724 $39,669Total Cost $8,603 $14,215 $365,363 $430,712 $34,768 $3,610 $857,271 $245,700Does Not Contain Confidential Business Information (CBI)

2011_March _Progress Report 6 Appendix B

Revised Certificate of Analysis for M+2 8-2 FTUCA Analytical StandardDoes Not Contain Confidential Business Information (CBI)

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