# Patent

## Title
Method for producing optically active 4-halo-3-hydroxybutyric acid ester

## Abstract

The present invention provides a method for preparing (S)-4-halo-3-hydroxybutyric acid ester comprises asymmetric reduction of 4-halo-acetoacetic acid ester using β-ketoacyl-acylcarrier protein reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid synthesis system. β-ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid synthesis system has a extremely high reducing activity as well as stereoseletivity for (S)-4-chloro-3-hydroxybutyric acid ester. In addition, the enzyme exhibits almost no oxidizing activity toward either configuration of ethyl 4-chloro-3-hydroxybutyrate, performing only the reducing reaction of ethyl, 4-chloroacetoacetate. Therefore, this enzyme can be used to efficiently produce (S)-4-halo-3-hydroxybutyric acid ester.

## Background
FIELD OF THE INVENTION
The present invention relates to a method for preparing (S)-4-halo-3-hydroxybutyric acid ester using β-ketoacyl-acylcarrier-protein reductase comprising Type II fatty acid synthase or acetoacetyl-CoA reductase comprising poly-β-hydroxy fatty acid. synthase.
BACKGROUND OF THE INVENTION
Known methods for preparing the optically active (S)-4-halo-3-hydroxybutyric acid ester include an asymmetric reduction method using 3-α-hydroxysteroid dehydrogenase (Japanese Patent Laid-Open Publication No. Hei 1-277494) and microorganisms such as baker's yeast (J. Am. Chem. Soc. 105, 5925-5926 (1983); Japanese Patent Laid-Open Publication No. Sho 61-146191). D-enzyme-1 and D-enzyme-2 have been reported as the enzyme of baker's yeast reducing 4-haloacetoacetic acid ester to produce (S)-4-halo-3-hydroxybutyric acid ester (J. Org. Chem. 56, 4778-4483 (1991)). Of these enzymes, D-enzyme-2 has been indicated to be the fatty acid synthase based on its molecular weight, etc. (J. Am. Chem. Soc. 107, 2993 (1985)).
However, in the synthesis of optically active (S)-4-halo-3-hydroxybutyric acid ester by reducing 4-haloacetoacetic acid ester using baker's yeast, the enzymatic activity is too low to produce the desired product in a high concentration. Furthermore, since baker's yeast has an enzyme reducing 4-haloacetoacetic acid ester to produce (R)-4-halo-3-hydroxybutyric acid ester, it is difficult to stably synthesize (S)-4-halo-3-hydroxybutyric acid ester with a high optical purity.
In addition, the fatty acid synthase mainly involved in the synthesis of (S)-4-halo-3-hydroxybutyric acid ester in baker's yeast has been reported to be quickly inhibited by SH reagents such as iodoaetamide, mercury, and p-(chloromercury)benzoic acid. Thus, the enzyme is expected to be inhibited by the substrate 4-haloacetoacetic acid ester and the product 4-halo-3-hydroxybutyric acid ester. Therefore, baker's yeast is not preferable for producing (S)-4-halo-3-hydroxybutyric acid ester in a large quantity.
It is also conceivable to synthesize (S)-4-halo-3-hydroxybutyric acid ester with a high optical purity by highly expressing the fatty acid synthase of baker's yeast with a high specific activity in heterologous microorganisms using genetic engineering techniques. However, the fatty acid synthase of baker's yeast is an extremely complex multicatalytic enzyme, in which α-subunit with the molecular weight of 208,000 consisting of 1,894 amino acid residues and β-subunit with the molecular weight of 229,000 consisting of 2,051 amino acid residues (J. Biol. Chem. 263, 12315-12325 (1988)) form an α6β6 complex (J. Biol. Chem. 253, 4464-4475 (1978)), having eight different activities, besides the β-keto group reducing activity (β-ketoacyl-ACP reducing activity), including the acyl carrier protein (ACP) activity, ACP-S-acetyltransferase activity, ACP-S-malonyl transferase activity, β-ketoacyl-ACP synthase activity, β-hydroxyacyl-ACP dehydrogenase activity, enoyl-ACP reductase activity, and palmitoyl transferase activity. Therefore, it is not easy to highly express this synthase in heterologous microorganisms. For example, an attempt to express FAS1 and FAS2 in minicells of E. coli reportedly resulted in failure to detect a full length of the enzymes (Ann. Rev. Biochem. 52, 537-579 (1983)).
The domain for the β-ketoacyl-ACP reducing activity which is expected to exhibit the 4-haloacetoacetic acid ester reductase activity has been indicated to be located in the α-subunit of the fatty acid synthase based on the amino acid sequence. It has been reported, however, that, when the α-subunit was completely dissociated by freeze-thawing in a high salt concentration (Biochem. J. 109, 312-314 (1968)) and by lysine modification with dimethyl maleic anhydride, the subunit alone did not express the β-ketoacyl-ACP reducing activity (Eur. J. Biochem. 94, 189-197 (1979)). It has also been reported that ethyl acetoacetate reducing activity was not expressed by the fatty acid synthase with an α6β6 structure but expressed only by that with an α2β2 structure (Mw 800,000) (Eur. J. Biochem. 172, 633-639 (1988)). Therefore, it has not been clarified which domain of the fatty acid synthase is essential for the 4-haloacetoacetic acid reducing activity and how to efficiently prepare the structure (α2β2) expressing the 4-haloacetoacetic acid ester reducing activity.
SUMMARY OF THE INVENTION
An objective of the present invention is to provide a method for efficiently producing (S)-4-halo-3-hydroxybutyric acid ester utilizing an enzyme constituting fatty acid synthase or that constituting the poly-β-hydroxy fatty acid biosynthetic system.
Fatty acid synthase is structurally classified into four types, IA, IB, IC, and II (Shin Seikagaku Jikken Koza (New Biochemical Experiment) 4, Shisitu (Lipids) I, p34-37). Animals including humans (Proc. Natl. Acad. Sci. USA 92, 8695 (1995)) have Type IA synthase comprising a homodimer (Mw about 500,000) of α-subunit (Mw about 250,000) having all of the above-described eight different activities of fatty acid synthase. Yeasts including baker's yeast and fungi have Type IB synthase of an α6β6 structure (Mw about 2,400,000) consisting of α-subunit (Mw about 210,000) and A-subunit (Mw about 200,000) expressing all the fatty acid synthase activities. Bacteria such as Brevibacterium ammoniagenes (Eur. J. Biochem. 247, 268 (1997)) and Micobacterium smegmatis (Physiol. Rev. 56, 339 (1976)) have Type IC synthase of an α6 structure consisting of α-subunit (Mw about 250,000) having all the fatty acid synthase activities. Plants such as Brassica napus (Biochim. Biophys. Acta, 1120, 151 (1992)) and algae, bacteria such as Esherichia coli, Actinomycetes, and viruses have Type II synthase in which individual reactions of the fatty acid synthase are carried out with separate enzyme proteins.
Taking notice of β-ketoacyl-ACP reductase classified into Type II fatty acid synthase, among these various types of enzymes, which is simpler in the structure and functions, smaller in molecular weight (Mw of subunit about 20,000 to 40,000) as compared with Type I enzymes (IA, IB, and IC), and not inhibited by SH reagents, the present inventors have thought that, if the enzyme has the activity to reduce 4-haloaetoaetic acid ester to synthesize (S)-4-halo-3-hydroxybutyric acid ester like Type IB fatty acid synthase of baker's yeast, it would be possible to produce (S)-4-halo-3-hydroxybutyric acid ester in a large quantity and create a microbial strain capable of producing (S)-4-halo-3-hydroxybutyric acid ester in high yield utilizing genetic engineering techniques.
Therefore, the present inventors have attempted the isolation of β-ketoacyl-ACP reductase constituting Type II fatty acid synthase to investigate its reducing activity toward 4-haloaetoaetic acid ester. Specifically, β-ketoacyl-ACP reductase genes of Escherichia coli or Bacillus subtilis, whose nucleotide sequences were known, were cloned by the polymerase chain reaction with chromosomal DNAs from respective bacteria as the template. The isolated gene was then introduced into Escherichia coli to highly express the enzyme therein to examine its 4-chloroacetoaetic acid ester reducing activity. As a result, the present inventors have found that the enzyme has an extremely high reducing activity and steroselectivity toward 4-chloroacetoacetic acid ester.
In addition, while it has been reported that β-ketoacyl-ACP reductase from Escherichia coil showed an oxidizing activity specific to the D-stereoisomer of β-hydroxybutyl-ACP (J. Biol. Chem. 240, 618-621 (1965)), the present inventors have found that the enzyme as well as the enzyme derived from Bacillus subtilis showed almost no oxidizing activity to 4-chloro-3-hydroxybutyric acid ester of either configuration, but only reducing ethyl 4-chloroacetoacetate. Such a property is extremely advantageous for synthesizing an optically active (S)-4-halo-3-hydroxybutyric acid ester by asymmetric reduction because the reaction equilibrium is not rate-limiting.
Furthermore, the present inventors have found that genes encoding acetoacetyl-CoA reductase (generally designated phbB or phaB), one of the enzymes constituting the poly-β-hydroxy fatty acid (PHA) biosynthesis system, have homology to that encoding Type II fatty acid synthase (generally designated fabG), and examined whether these acetoacetyl-CoA reductases are capable of asymmetrically reducing 4-chloroacetoacetic acid ester to (S)-4-halo-3-hydroxybutyric acid ester like β-ketoacyl-ACP reductase. Specifically, the inventors isolated the acetoacetyl-CoA reductase gene from Ralstonia eutropha, introduced the resulting gene to Escherihia coli to express it, and used the expressed acetoacetyl-CoA reductase to reduce 4-chloroacetoacetic acid ester. As a result, it has been found that the enzyme has a high reducing activity and stereoselectivity for producing (S)-4-chloro-3-hydroxybutyric acid ester.
The inventors also have found that acetoacetyl-CoA reductase, like β-ketoacyl-ACP reductase, shows almost no reactivity to either optical isomer of 4-chloro-3-hydroxybutyric acid ester, and practically functions as only the reductase, which is favorable to the synthesis of (S)-4-chloro-3-hydroxybutyric acid ester.
Furthermore, the present inventors have attempted to efficiently accelerate the reducing reaction cycle by regenerating the coenzyme (NADPH or NADH), which is consumed associated with the asymmetric reduction of 4-chloroacetoacetic acid ester in this reducing reaction system. First, the inventors introduced into Escherihia coli a gene encoding glucose dehydrogenase capable of regenerating the coenzyme (NADPH or NADH) and a gene encoding β-ketoacyl-ACP reductase, thereby obtaining E. coli strain capable of producing these enzymes. Then, ethyl 4-haloacetoacetate was reduced utilizing the thus-obtained transformant to measure the yield and the optical purity of ethyl (S)-chloro-3-hydroxybutyrate thus produced. As a result, it was found that the recombinant enzyme produced by the Escherihia coli strain has a high enzymatic activity quite suitable for producing ethyl (S)-chloro-3-hydroxybutyrate with a high optical purity.
The present invention relates to a method for producing (S)-4-halo-3-hydroxybutyric acid ester by reacting 4-halo-acetoacetic acid ester or its derivatives with β-ketoacyl-ACP reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase, one of the enzymes constituting the poly-β-hydroxy fatty acid biosynthesis system. More specifically, it relates to:

    (1) a method for producing (S)-4-halo-3-hydroxybutyric acid ester comprising asymmetrically reducing 4-halo-acetoacetic acid ester or its derivatives with β-ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase,
    (2) the method of (1), wherein said β-ketoacyl carrier protein reductase is derived from Escherihia coli, 
    (3) the method of (1), wherein said β-ketoacyl carrier protein reductase is selected from the group consisting of:
    (a) a protein comprising the amino acid sequence of SEQ ID NO: 1;
    (b) a protein comprising a modified amino acid sequence of SEQ ID NO: 1 in which one or more amino acid residues are added, deleted, or substituted and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester; and
    (c) a protein encoded by DNA hybridizable with the DNA comprising the nucleotide sequence of SEQ ID NO: 2 and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester,
    (4) the method of (1), wherein said β-ketoacyl-acyl carrier protein reductase is derived from microorganisms belonging to the genus Bacillus, 
    (5) the method of (4), wherein said β-ketoacyl-acyl carrier protein reductase is derived from Bacillus subtilis, 
    (6) the method of (1), wherein said β-ketoacyl-acyl carrier protein reductase is selected from the group consisting of:
    (a) a protein comprising the amino acid sequence of SEQ ID NO: 5;
    (b) a protein comprising a modified amino acid sequence of SEQ ID NO: 5 in which one or more amino acid residues are added, deleted, or substituted and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester; and
    (c) a protein encoded by DNA hybridizable with the DNA comprising the nucleotide sequence of SEQ ID NO: 6 and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester,
    (7) a method for producing (S)-4-halo-3-hydroxybutyric acid ester comprising asymmetrically reducing 4-halo-acetoacetic acid ester or its derivatives with acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid biosynthesis system,
    (8) a method of (7), wherein said acetoacetyl-CoA reductase is derived from microorganisms belonging to the genus Ralstonia, 
    (9) a method of (8), wherein said acetoacetyl-CoA reductase is derived from Ralstonia eutropha, 
    (10) the method of (7), wherein said acetoacetyl-CoA reductase is selected from the group consisting of:
    (a) a protein comprising the amino acid sequence of SEQ ID NO: 9;
    (b) a protein comprising a modified amino acid sequence of SEQ ID NO: 9 in which one or more amino acid residues are added, deleted, or substituted and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester; and
    (c) a protein encoded by DNA hybridizable with the DNA comprising the nucleotide sequence of SEQ ID NO: 10 and capable of asymmetrically reducing 4-haloacetoacetic acid ester or its derivatives to produce (S)-4-halo-3-hydroxybutyric acid ester,
    (11) the method of (1) or (7), wherein said 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester,
    (12) the method of (1) or (7), wherein said 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate,
    (13) the method of (1),wherein said method uses a microorganism capable of producing β-ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase and an enzyme catalyzing production of NAD(P)H from NAD(P)+,
    (14) the method of (13), wherein said microorganism is a recombinant microorganism into which heterologous or homologous DNA encoding the β-ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase and heterologous or homologous DNA encoding an enzyme catalyzing production of NAD(P)H from NAD(P)+, and is capable of expressing both enzymes,
    (15) the method of (14), wherein said microorganism is Escherihia coli, 
    (16) the method of any one of (13) through (15), wherein said enzyme catalyzing production of NAD(P)H from NAD(P)+ is glucose dehydrogenase,
    (17) the method of (7), wherein said method uses a microorganism capable of producing acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid biosynthesis system and an enzyme catalyzing production of NAD(P)H from NAD(P)+,
    (18) the method of (17), wherein said microorganism is a recombinant microorganism into which heterologous or homologous DNA encoding acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid biosynthesis system and heterologous or homologous DNA encoding an enzyme catalyzing production of NAD(P)H from NAD(P)+ and capable of expressing both enzymes,
    (19) the method of (18), wherein said microorganism is Escherichia coli, and
    (20) the method of any one of (17) through (19), wherein said enzyme catalyzing production of NAD(P)H from NAD(P)+ is glucose dehydrogenase.





BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the restriction map of plasmid pSE420D. P(trc) represents the trc promoter, T(rrnB) the rrnB T1T2 terminator, Amp the β-lactamase gene for ampicillin resistance, and ori the replication origin of plasmid.
FIG. 2 shows the restriction map of plasmid pSG-ECR1. P(trc) represents the trc promoter, EcfabG the β-ketoacyl ACP reductase gene derived from Escherichia coli, BsG1cDH the glucose dehydrogenase gene derived from Bacillus subtilis, T(rrnB) the rrnB T1T2 terminator, Amp the β-lactamase gene for ampicillin resistance, and ori the replication origin of plasmid.
FIG. 3 shows the restriction map of plasmid pSG-BSR1. P(trc) represents the trc promoter, BcfabG the β-ketoacyl ACP reductase gene derived from Bacillus subtilis, BSG1cDH the glucose dehydrogenase gene derived from Bacillus subtilis, T(rrnB) the rrnB T1T2 terminator, Amp the β-lactamase gene for ampicillin resistance, and ori the replication origin of plasmid.
FIG. 4 shows the restriction map of plasmid pSG-AER1. P(trc) represents the trc promoter, AephbB the aetoacetyl-CoA reductase gene derived from Ralstonia eutropha, BsG1cDH the glucose dehydrogenase gene derived from Bacillus subtilis, T(rrnB) the rrnB T1T2 terminator, Amp the β-lactamase gene for ampicillin resistance, and ori the replication origin of plasmid.



DETAILED DESCRIPTION OF THE INVENTION
The method for producing (S)-4-halo-3-hydroxybutyric acid ester of the present invention uses the enzyme constituting Type II fatty acid synthase (EC1.1.1.100) or acetoacetyl-CoA reductase (EC 1.1.1.36) constituting the PHA biosynthesis system. The enzyme (EC 1.1.1.100) constituting Type II fatty acid synthase is simpler in the structure and functions, smaller in the molecular weight (Mw of subunit about 20,000 to 40,000), and no inhibited by SH reagents as compared with Type IA synthase comprising a homodimer of α-subunit, Type IB synthase of an α6β6 structure consisting of α-subunit and β-subunit, and Type IC synthase of an α6 structure of α-subunit, which have all of various activities of fatty acid synthase. Therefore, Type II synthaseiis advantageous and preferable for large quantity production of (S)-4-halo-3-hydroxybutyric acid ester and construction of a microbial strain capable of high level producion of (S)-4-halo-3-hydroxybutyric acid ester.
The source of the synthase used is not particularly limited. In addition to β-ketoacyl-ACP reductase derived from Escherichia coli (:SEQ ID NO: 1, J. Biol. Chem. 267, 5751-5754 (1992)) and that derived from Bacillus subtilis (SEQ ID NO: 5, J. Bacteriol. 178, 4794-4800 (1996)), and enzymes derived from other various organisms can be used. The enzymes derived from other organisms include β-ketoacyl-ACP reductase derived from Actinobacillus actinomycetemcomitans (Biochem. Biophys. Res. Commun, 230, 220-225 (1997)), Bacillus subtilis (J. Bacteriol. 178, 4794-4800 (1996)), Escherichia coli (J. Biol. Chem. 267, 5751-5754 (1992)), Mycobacterium bovis (Science 267, 227-230 (1994)), Mycobacterium smegmatis (DDBJ Accession number U66800), Mycobacterium tuberculosis (Mol. Microbiol. 15, 1009-1015 (1995)), Propionibacterium shermanii (J. Gen. Microbiol. 127, 121-129 (1981)), Streptococcus pneumoniae (WO97/43303), Synechocystis sp. (DNARes. 3, 109-136 (1996)), Thermotoga maritima (J. Bacteriol. 178, 248-257 (1996)), Vibrio harveyi (J. Bacteriol. 178, 571-573 (1996)), Haemophilus influenza (Science 269, 469-512 (1995)), etc. β-ketoacyl-ACP reductases derived from plants include those derived from Allium porrum (Plant Physiol. 115, 501-510 (1997)), Arabidopsis thaliana (Biochem. J. 283, 321-326 (1992), Plant Physiol. 115, 501-510 (1997)), Brassica napus (WO96/02652)), Carthamus tinctorius (Arch. Biochem. Biophys. 217, 144-154 (1982)), Cuphea lanceolata (Mol. Gen. Genet. 233, 122-128 (1992)), Hordeum vulgare (Plant Physiol. 115, 501-510 (1997)), Persea americana (Biochem. J. 271, 713-720 (1990)), carrot (Arch. Biochem. Biophys. 300, 157-163 (1993)), Euglena gracilis (J. Biol. Chem. 255, 1504-1508 (1980)), Spinacia oleracea (Plant Physiol. 69, 1257-1262 (1982)), Zea mays L. (Plant Physiol. 115, 501-510 (1997)), etc.
A gene encoding β-ketoacyl-ACP reductase can be isolated utilizing, for example, hybridization techniques. A β-ketoacyl-ACP reductase gene derived from various organisms can be isolated by hybridization, under the stringent conditions, with the DNA encoding β-ketoacyl-ACP reductase derived from Escherichia coli (SEQ ID NO: 2) and that from Bacillus subtilis (SEQ ID NO: 6), or DNAs prepared from other organisms using the portions thereof as the probe. The polymerase chain reaction can also be utilized to isolate the desired gene. For example, primers are designed based on the highly homologous region in the gene encoding β-ketoacyl-ACP reductase (e.g. NADPH-binding region, 10th to 34th amino acid residues of β-ketoacyl-ACP reductase from Escherichia coli) and, using the resulting primers and the chromosomal DNA or CDNA of a target organism as the template, the polymerase chain reaction is performed to isolate the gene encoding β-ketoacyl-ACP reductase from various organisms.
Poly-β-hydroxy fatty acid (PHA) is known to accumulate in more than 100 varieties of prokaryotic microorganisms including the genus Alcaligenes, Aphanothece, Azotobacter, Bacillus, Pseudomonas, Rhodospirillum, and Actinomyces. The PHA biosynthetic system comprises 3-ketothiolase, acetoacetyl CoA reductase and PHA synthase. Among them, acetoacetyl CoA reductase comprises a tetramer of the subunit with Mw 20,000 to 40,000 and preferably uses reduced nicotinamide adenine dinuleotide phosphate (NADPH) as the coenzyme for the reducing reaction. It can also employ a more economical and highly stable reduced nicotinamide adenine dinucleotide (NADH) as the coenzyme and thus is industrially advantageous.
Any acetoacetyl CoA reductases can be used in the present invention regardless of their origin so far as they are acetoacetyl CoA reductase participating in the PHA biosynthesis. Examples thereof include acetoacetyl CoA reductase derived from Acinetobacter sp. RA3849 (J. Bacteriol. 177, 4501-4507 (1995)), Ralstonia eutropha (previously called Alcaligenes eutrophus, FEMS Microbiol. Lett. 52, 259-264(1988)), Alcaligenes latus (J. Microbiol. Biotechnol. 6, 425-431 (1996)), Alcaligenes sp. SH-69 (DDBJ Accession No. AF002014), Azospirillum brasilense (J. Gen. Microbiol. 136, 1191-1196 (1990), Mol. Gen. Genet. 231, 375-384 (1992)), Azotobater beijerinckii (Biochem. J., 134, 225-238 (1973)), Bacillus megaterium (Can. J. Microbiol. 41 (Suppl. 1), 77-79 (1995)), Chromatium vinosum D strain (Eur. J. Biochem. 209, 135-150 (1992)), Ectothiorhodospira shaposhnikovii (Appl. Microbiol. Biotechnol. 40, 292-300 (1993)), Lupinus luteus (Plant Soil, 56, 379-390 (1980)), Methylobacterium extorquens (FEMS Microbiol. Lett. 156, 275-279 (1997)), Methylobacterium rhodesianum MB 126 (Arch. Microbiol. 161, 277-280 (1994)), Paracoccus denitricans (FEMS Microbiol. Rev. 103, 257-264 (1992), FEMS Microbiol. Lett. 133, 85-90 (1995)), Pseudomonas sp. (DDBJ Accession No. Z80156), Rhizobium lupini (Fiziol. Rast. (Moscow) 27, 544-550 (1980)), Rhizobium meliloti 41 or Sinorhizobium meliloti (Microbiology, 141, 2553-2559 (1995)), Rhodococus ruber NCIMB 40126 (FEMS Microbiol. Rev., 103, 93-101 (1992)), Synechococcus sp. (Japanese Patent Laid-open Publication No. Hei 6-187085), Syntrophomonas wolfei subsp. wolfei (Arch. Microbiol. 159, 16-20 (1993)), Thiocapsa pfennigii (Appl. Microbiol. Biotechnol. 40, 292-300 (1993)), and Zoogloea ramigera I-16-M (Arch. Microbiol. 114, 211-217 (1977), J. Biol. Chem. 262, 97-102 (1987)).
If the nucleotide sequence of the gene encoding acetoacetyl-CoA reductase is known, the coding region can be isolated using the polymerase chain reaction (PCR). The acetoacetyl-CoA reductase gene can also be isolated from various organisms by hybridizing, under the stringent conditions, the target DNA with DNA encoding acetoacetyl-CoA reductase derived from, for example, Ralstonia eutropha (SEQ ID NO: 10, the amino acid sequence of the enzyme is shown in SEQ ID NO: 9) or DNA preprared from other organisms using the portions of the above enzyme-encoding DNA as the probe. Furthermore, it is also possible to isolate the acetoacetyl-CoA reductase gene from various organisms utilyzing the polymerase chain reation (PCR) with primers designed based on a highly homologous region (such as the NADPH-binding region) of the gene encoding acetoacetyl-CoA reductase and the chromosomal DNA or CDNA from a target organism as the template.
In the method of the present invention, may be used not only naturally-occurring enzymes but also variant enzymes having modified amino acid sequence of the natural enzyme so far as the variant enzymes are functionally equivalent to the natural enzyme. Such modification of the amino acid sequence can be made by the method using BAL31 exonulease III, Kunkel method, and PCR, which are well known to those skilled in the art (Labomanual Genetic Engineering, 3rd ed., p219-230, Maruzen). Since the amino acid substitution may occur spontaneously, not only enzymes having amino acid artificially modified but also enzymes with spontaneously modified amino acid sequence can be used in the present invention.
In addition, the enzyme genes used in the method of the present invention include the gene having homology to β-ketoacyl-ACP reductase or acetoacetyl-CoA reductase and encoding Type II synthase of polyketides biosynthesized through the system similar to that of fatty acid synthesis and PHB synthesis (e.g. the open reading frame (ORF)-3 gene of the actI gene derived from Sachropolyspora hirsuta, Mol. Gen. Genet. 240, 146-150 (1993)), ORF-5 in the gene having homology to the above actI gene derived from Streptomyces cinnamonensis and putatively participating in the biosynthesis of monensin (Mol. Gen. Genet. 234, 254-264 (1992)), actIII that is a gene for actinorhodin biosynthesis derived from Streptomyces coelicolor (Gene 74, 305-320 (1988)), aknA that is a gene for Aklavinone biosynthesis derived from Streptomyces galilaeus (J. Bacteriol., 176, 2473-2475 (1994)), dauB that is a gene for daunomyin biosynthesis derived from Streptomyces sp. C5 (J. Bacteriol., 176, 6270-6280 (1994)), ORF-5 of the gene for granaticin polyketide synthase putative ketoacyl reductase 1 derived from Streptomyces violaceoruber Tu22, ORF-6 of the gene for the granaticin polyketide synthase putative ketoacyl reductase 2 (EMBO J., 8, 2717-2725 (1989), etc.), nodG that is a gene involved in the nodulation derived from Rhizobium meliloti RCR2011 (Nucleic Acids Res. 14, 7453-7472 (1986)), and hetN that is a gene involved in the heterocyst formation derived from Anabaena sp. (PCC 7120) (J. Bacteriol. 176, 2282-2292 (1994)). These can be preferably used as long as enzymes (such as β-ketoacyl reductase), products of these genes, have the activity to reduce 4-halo-acetoacetic acid ester to produce (S)-4-halo-3-hydroxybutyric acid.
In the present invention, “asymmetric reduction of 4-halo-acetoacetic acid ester or its derivatives using β-ketoacyl-acyl carrier protein (ACP) reductase or acetoacetyl-CoA reductase” is not necessarily perfomed using the purified enzyme. Microorganisms and plants containing said enzyme, or thier treated products can also,be used. Especially, it is preferable to use organisms transformed with a heterologous or homologous gene encoding β-ketoacyl-ACP reductase or acetoacetyl-CoA reductase using genetic engineering techniques to enable high level expression of the enzyme or the treated products, of the organisms. If the organism having β-ketoacyl-ACP reductase or acetoacetyl-CoA reductase also possesses the enzyme reducing 4-halo-acetoacetic acid ester or its derivatives to synthesize (R)-4-halo-3-hydroxybutyric acid ester, such a organism is preferably mutated to delete these (R)-enatiomer generating enzymes by the natural or artificial mutation or recombinant DNA techniques.
The host microorganisms used the present invention are not particularly limitated as long as they can be transformed with DNA encoding the polypeptide having the activity of β-ketoacyl-ACP reductase or acetoacetyl-CoA reductase and express these enzyme activities. Specific examples thereof include bacteria for which the host vector system has been developed, such as the genera Escherichia, Bacillus, Pseudomonas, Serratia, Brevibacterium, Corynebacterium, Streptococcus, and Lactobacillus, yeasts such as Saccharomyces, Kluyveromyces, Schizosaccharomyces, Zygosaccharomyces, Yarrowia, Trichosporon, Rhodosporidium, Hansenula, Pichia, and Candida, and fungi such as the genera Neurospora, Aspergillus, Cephalosporium, and Trichoderma. 
Transformants can be prepared by techniques conventionally used in the field of molecular biology, biotechnology, and genetic engineering (e.g., Sambrook et al., Molocular Cloning, Cold Spring Harbor Laboratories). In order to express the gene for β-ketoacyl-ACP reductase or acetoacetyl-CoA reductase of the present invention in mirooragnisms and the like host, it is necessary to first introduce the gene into a plasmid or phage vector that can be stably present in miroorganisms to transcript and translate the genetic information. For that purpose, as the unit for controlling the transcription and translation, a promoter can be incorporated upstream of the 5′-side of the gene and a terminator downstream of the 3′-side of the gene. Any promoter and terminator can be used so far as they function in microorganisms to be used as the host. Vetors, promoters, and terminaters functioning in various microorganisms are described in detail in “Biseibutugaku Kisokoza (Fundamental Microbiology), 8, Idenshikogaku (Genetic Engineering), Kyoritsu”, especially those usable in yeast in Adv. Biochem. Eng. 43, 78-102 (1980) and Yeast 8, 423-488 (1992).
For example, in the genus Escherichia, especially in Esherichia coli, pBR and pUC series can be used as the plasmid vector. Examples of the promoters include those derived from lac (β-galactosidase), trp (tryptophan operon), tac (lac and trp fused), phage λ PL, and PR. The terminators include trpA terminator and rrnB ribosomal terminator.
In the genus Bacillus, the plasmid pUB110 pC194 series can be used as the vector. These vectors can be integrated into chromosomes. as the promoter and the terminator, those for apr (alkaline protease), npr (neutral protease), and amy (.-amylase) can be used.
In the genus Pseudomonas, the host-vector system has been developed in Pseudomonas putida, Pseudomonas cepacia, etc. Vectors with a broad host spetrum such as pKT240 (comprising the gene required for the autonomous replication derived from RSF1010 or the like) developed based on the plasmid TOL participating in the degradation of toluene compounds can be used. An example of the promoter and terminator that can be used is those for the lipase gene (Japanese Patent Laid-Open Publication No. Hei 5-284973).
In the genus Brevibacterium, especially in Brevibacterium lactofermentum, the plsamid vector such as pAJ43 (Gene 39, 281 (1985)) can be used. In this vector, the promoters and terminators employed in Escherichia coli can be used.
In the genus Corynebacterium, especially in Corynebacterium glutamicum, the plasmid vectors such as pCS11 (Japanese Patent Laid-Open Publication No. Sho 57-183799) and pCB101 (Mol. Gen. Genet. 196, 175 (1984)) can be used.
In the genus Streptococcus, pHV1301 (FEMS Microbiol. Lett. 26, 239 (1985)) and pGK1 (Appl. Environ. Microbiol. 50, 94 (1985)) can be used as the plasmid vector.
In the genus Lactobacillus, vector pAMβ1 (J. Bacteriol. 137, 614 (1979)) developed for the genus Streptococcus and the promoter used in Escherichia coli can be employed.
In the genus Saccharomyces, especially in Saccharomyces cerevisiae, the plasmid YRp series, YEp series, YCp series, and YIp series can be used. The integration vector that utilizes the homologous recombination with the ribosomal RNA present in multicopies in A chromosome (EP 537456) is extremely useful because it allows to integrate muticopies of a gene and stably maintain them. The promoters and terminators that can be used in this yeast are those for ADH (alcohol dehydrogenase), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), PHO (acidic phosphatase), GAL (β-galactosidase), PGK (phosphoglycerate kinase), and ENO (enolase).
In the genus Kluyveromyces, especially in Kluyveromyces lactis, examples of the vectots include the plasmid 2 μm series derived from Saccharomyces cerevisiae, pKD1 series (J. Bacteriol. 145, 382-390 (1981)), plasmids derived from pKG11 concerned with the killer activity, KARS series which is the autonomous replication gene in the genus Kluyveromyces, and the vector plasmid capable of integrating to chromosome by the homologous recombination with ribosomal RNA (EP 537456). Promoters and terminators derived from ADH, PGK, etc. can be used.
In the genus Schizosaccharomyces, the vectors to be used are ARS (the autonomous replication-related gene) derived from Schizosaccharomyces pombe and the plasmid vector containing the selection marker complementing the autotrophy derived from Saccharomyces cerevisiae (Mol. Cell Biol. 6, 80 (1986)). ADH promoter derived from Schizosaccharomyces pombe can be used (EMBO J. 6, 729 (1987)).
In the genus Zygosaccharomyces, the plasmid vector based on pSB3 (Nucleic Aids Res. 13, 4267 (1985)) derived from Zygosaccharomyces rouxii can be used. Examples of the promoters are PHO5 promoter derived from Saccharomyces cerevisiae, GAP-Zr (glyceraldehyde-3-phosphate dehydrogenase) promoter derived from Zygosaccharomyces rouxii (Agri. Biol. Chem. 54, 2521 (1990)).
In the genus Hansenula, the host vector has been developed in Hansenula polymorpha. The autonomous replication-related genes HARS1 and HARS2 derived from Hansenula polymorpha can be used as the vector. Since they are relatively unstable, the multicopy integration to chromosome is effective (Yeast 7, 431-443 (1991)). AOX promoter (alcohol oxidase) induced by methanol and FDH (formate dehydrogenase) promoter can be used.
In the genus Pichia, the host-vector system has been developed in Pichia pastoris utilizing the genes (PARS1 and PARS2) involved in the autonomous replication for Pichia (Mol. Cell. Biol. 5, 3376 (1985)). In this vector, the potent promoter such as AOX inuducible by the high concentration culture and methanol can be used (Nucleic Acids Res. 15, 3859 (1987)).
In the genus Candida, the host-vector system has been developed in Candida maltosa, Candida albicans, Candida tropicalis, Candida utilis, Candida boidinii, etc (Japanese Patent Laid-Open Publication No. Hei 5-344895). Candida maltosa-derived ARS has been cloned (Agri. Biol. Chem. 51, 1587 (1987)) and used to develop a vector. In Candida utilis, a potent promoter has been developed for the vector capable of chromosomal integration (Japanese Patent Laid-Open Publication No. Hei 8-173170).
In the genus Aspergillus, Aspergillus niger, Aspergillus oryzae, etc. have been most comprehensively studied among funji. Plasmids derived from these species and vectors capable of chromosomal integration are available. Promoters for the extracellular proteases and amylases can be used (Trends in Biotehnology 7, 283-287 (1989)).
In the genus Trichoderma, the Trichoderma reesei-based host-vector system has been developed. In this vector the promoter for the extracellular cellulase gene can be used (Biotechnology 7, 596-603 (1989)).
The culturing of transformants and purification of recombinant proteins from transformants can be performed by the conventional method known to those skilled in the art.
The substrate for the enzyme in the present invention is 4-halo-acetoacetic acid ester or its derivatives such as those with a substituent at the α-position. Preferable substrates are 4-chloroacetoacetic acid ester and ethyl 4-chloroacetoacete. The substrate concentration used in the method of this invention ranges usually from 0.1 to 50%, preferably from 1 to 20%. The enzyme amount used ranges usually from 0.01 to 500 U/ml of the reaction mixture, preferably from 0.1 to 50 U/ml. The coenzyme (NADPH or NADH) required by the enzyme is added in the reaction system if necessary in an amount of 0.00001 to 5 equivalents, preferably 0.0001 to 1 equivalent, with respect to the amount of the substrate. A buffer solution (for example, a phosphate buffer) can be used as a solvent in the reaction system to maintain the pH. A aqueous two-phase reaction system containing 10 to 90% organic solvent such as octane, hexane, toluene, ethyl acetate, n-butyl acetate, and chloroform can also be used. The reaction temperture is usually 4 to 50° C., preferably 10 to 30° C. pH is adjusted to usually 3 to 9, preferably 4 to 8. The reaction product, (S)-4-halo-3-hydroxybutyric acid ester, can be extracted by an organic solvent capable of dissolving the product well such as ethyl acetate and octane and purified by a method such as distillation.
In the above-described reaction, NADP+ produced from NADPH during the reducing reaction is suitably converted to NADPH (acetoacetyl-CoA reductase and β-ketoacyl reductase involved in the biosynthesis of PHA and polyketides utilize not only NADPH but also NADH). The regeneration of the coenzymes can be performed utilizing the NAD(P)+ reducing ability (such as the glycolytic pathway) of miroorganisms. The NAD(P)+ reducing ability can be increased by supplementing the reaction system with glucose or ethanol or by adding a microorganism capable of generating NAD(P)H from NAD(P)+, treated products thereof, or the enzyme with such an activity. For example, NAD(P)H can be regenerated using microorganisms having glucose dehyrogenase, malate dehydrogenase, glutamate dehydrogenase, formate dehydrogenase, alcohol dehydrogenase, amino acid dehydrogenase, and/or glycerol dehydrogenase, treated products thereof, or the purified enzymes. Furthermore, the microorganism capable of producing β-ketoacyl reductase or acetoacetyl-CoA reductase can be genetically engineered to highly express these enzymes with the NAD(P)H regenerating activity, and the resulting transformant or the treated product thereof can be used in the method of the present invention.
In the present invention, it is also possible to use microorgansims capable of producing both acetoacetyl-CoA reductase and the NAD(P)H regenerating enzyme or both β-ketoacyl reductase and the NAD(P)H regenerating enzyme, or the treated products thereof. Such microorganisms can further genetically engineered to highly express these enzymes and the resulting microorganisms or their treated products can be used in the method of the present invention.
In the method of producing (S)-4-halo-3-hydroxybutyric acid ester using the microorganism capable of producing both acetoacetyl-CoA reductase and the NAD(P)H regenerating enzyme or both β-ketoacyl reductase and the NAD(P)H regenerating enzyme, or the treated products thereof, 1/50 to 1/20 volume, preferably 1/10 to 1/5 volume, based on the reaction mixture, of the culture, microbial cells recovered from the culture, or treated products thereof can be used. NAD+ or NADP+ can be added to the reaction mixture if necessary in an amount of 0.00001 to 5 equivalents, preferably 0.0001 to 1 equivalent, with respect to the amount of the substrate.
The reaction can also be performed using a bioreactor in which the microorganism producing the two enzymes or treated products thereof is immobilized on κ-carrageenan, acrylamide, polyurethane, chitin, or the like carrier. The reaction using the bioreactor can be performed by continuously supplying 4-halo-acetoacetic acid ester as the substrate, the substrate for regenerating the coenzyme (e.g. glucose when glucose dehydrogenase is used as the enzyme for regenerating the coenzyme), and if required, a buffer for controlling pH, and NAD+ or NADP+ as the coenzyme, or repeatedly using the reaction mixture.
The present invention provides a method of producing the optically active (S)-4-halo-3-hydroxybutyric acid ester using β-ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase constituting the poly-β-hydroxy fatty acid biosynthesizing system. β-Ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase is especially advantageous for constructing a highly productive system using genetic recombination techniques, enabling a more efficient production of the optically active (S)-4-halo-3-hydroxybutyric acid ester as compared with the prior art techniques.
The present invention will be described in more detail with reference to examples, but is not construed to be limited to these examples.
EXAMPLE 1
Isolation of β-Ketoacyl-ACP Reductase Gene from Escherichia coli 
Escherichia coli JM109 strain was cultured in the LB medium (containing Bacto-Tryptone 10 g, Bacto-Yeast extract 5 g, and NaCl 10 g/l) and the chromosomal DNA was prepared from the bacteria thus obtained using a Qiagen Genomic-tip (Qiagen). For the PCR cloning of the β-ketoacyl-ACP reductase gene (fabG) (J. Biol. Chem. 267, 5751-5754 (1992)) of Escherichia coli, the primers ECR-ATG1 (5′-AAAGGATCCAACAATGAATTTTGAAGGAAAAATCGC-3′, SEQ ID NO: 3) and ECR-TAG1 (5′-TGCCTCGAGTTATCAGACCATGTACATCCCGC-3′, SEQ ID NO: 4) were synthesized based on the nucleotide sequences at the 5′- and 3′-ends of the structural gene. Using the chromosomal DNA of Escherichia coli as the template, and the ECR-ATG1 and ECR-TAG1 primers, 30 cycles of PCR (95° C. for 30 sec, 50° C. for 1 min, and 75° C. for 2 min) were performed to obtain the amplified DNA fragments.
The resulting DNA fragments were digested with restriction enzymes BamHI and XhoI. The plasmid vector pSE420 (Invitrogen) was g digested with NcoI and BamHI, treated with the Klenow fragment, and subjeted to the self-cyclyzation reation to obtain the plasmid pSE420B. This pSE420B was digested with BamHI and XhoI, and ligated to the above PCR amplified fragments digested with the same two restriction enzymes using T4 DNA ligase to obtain the plasmid pSE-ECRI. The DNA insert in the plasmid thus obtained were sequenced and identified as the fabG gene. The nucleotide sequence of fabg is shown in SEQ ID NO: 2 and the amino acid sequence of the protein encoded by the gene in SEQ ID NO: 1.
EXAMPLE 2
Expression of β-Ketoacyl-ACP Reductase Gene from Escherichia coli 
Escherichia coli HB101 strain was transformed with pSE-ECR1 and the resulting transformant (E. coli HB101 (pSE-ECR1)) was cultured in the LB medium (5 ml) containing ampicillin (50 mg/ml) overnight. IPTG was added to the medium to 0.1 mM and the culture was further incubated for 5 h. Bacterial cells thus obtained were collected, disrupted with a Minibeatbeater 8 (BIOSPEC), and centrifuged to obtain the supernatant as the cell-free extract.
EXAMPLE 3
Reducing Activity of β-Ketoacyl-ACP Reductase from Escherichia coli 
The reducing activity of the cell-free extract obtained in Example 2 was assayed using ethyl 4-chloroacetoacetate, ethyl acetoacetate, and acetoacetyl-CoA as the substrate.
A reaction mixture containing 50 mM potassium phosphate buffer (pH 6.5), 0.2 mM NADPH, and 20 mM the substrate (0.2 mM when acetoacetyl-CoA was used as the substrate) and the enzyme was allowed to react at 25° C. One unit of the enzyme was defined as the amount of the enzyme catalyzing the decrease of 1 μmol of NADPH for 1 min. The results are shown in Table 1. In this table, ECAA represents ethyl 4-chloroacetoacetate, EAA ethyl acetoacetate, and AASCoA acetoacetyl-CoA, R the reducing activity, and DH the dehydrogenase activity.













TABLE 1







NADPH-
NADPH-
NADPH-
NADP+-R-
NADP+-S-



ECAA-R
AASCoA-R
EAA-R
ECHB-DH
ECHB-DH





















Cell-
100%
116%
1.90%
0.010%
0.004%


free


ex-


tract









The cell-free extract expressing β-ketoacyl-ACP reductase showed the NADPH-dependent ethyl 4-chloroacetoacetate-reducing activity, and its specific activity was 0.90 U/mg protein. In contrast, the HB101 strain containing no plasmid pSE-ECR1 showed almost no ethyl 4-chloroacetoacetate-reducing activity. The extract showed approximately the same activity for acetoacetyl-CoA as that for ethyl 4-chloroacetoacetate, while almost no activity for ethyl acetoacetate having no chlorine group.
EXAMPLE 4
Oxidizing Activity of β-Ketoacyl-ACP Reductase Derived from Escherichia coli 
The oxdizing activity of the cell-free extract obtained in Example 2 was assayed using ethyl (S)- or (R)-4-chloro-3-hydroxybutyrate as the substrate. The oxdizing reaction was performed by incubating a reaction mixture containing 50 mM Tris-HCl buffer (pH 9.0), 2.5 mM NADP+, 20 mM the substrate, and the enzyme at 25° C. One unit of enzyme was defined as the amount of enzyme catalyzing the increase of 1 μmol of NADPH for min. The results are shown in Table 1. As shown in Table 1, the extract showed almost no oxidizing activity for either substrate. In this table, ECHB represents ethyl 4-chloro-3-hydroxybutyrate.
EXAMPLE 5
Stereoseletivity of β-Ketoacyl-ACP Reductase Derived from Escherichia coli for Ethyl 4-chloro-3-hydoxybutyrate
A reaction mixture (1 ml) containing 200 mM potassium phosphate buffer (pH 6.5), 146 mM NADPH, 2% ethyl 4-chloroacetoacetate (122 mM), and β-ketoacyl-ACP reductase (2 U) prepared in Example 2 was incubated at 20° C. overnight. Aliquot of the reaction solution was diluted 2-fold with 0.1 N HCl and ethyl 4-chloro-3-hydroxybutyrate contained in the diultion was determined by gas chromatography. Gas chromatography was performed with a Thermon 3000 chromosolve W (2 m, Shinwakako) under the conditions of the column temperature of 150° C. and the detection temperature of 250° C. using a flame ionization detector (FID). As a result, the concentration of ethyl 4-chloro-3-hydroxybutyrate was found to be 13.3 g/l with a yield of 66.3%.
Optical purity was assayed by extracting ethyl 4-chloro-3-hydroxybutyrate from the reaction mixture with ethyl acetate, removing the solvent, and subjecting the residue to the high-performance liquid chromatography using an optical resolution column (column, chiracel OD (Daicel Chemical)); mobile phase, n-hexane/isopropanol (9/2); RI detection; flow rate, 0.5 ml/min). As a result, the optical purity was 96.5% ee (S) or more.
EXAMPLE 6
Synthesis of Ethyl 4-chloro-3-hydoxybutyrate by β-Ketoacyl-ACP Reductase Derived from Escherichia coli 
A reaction solution (1 ml) containing 200 mM potassium phosphate buffer solution (pH 6.5), 2% ethyl 4-chloro-acetoacetate (122 mM), 1.0 mM NADPH, β-ketoacyl-ACP reductase (1 U), 250 mM glucose, and glucose dehydrogenase (Wako Pure Chemical) (10 U) was incubated at 25° C. for 16 h. Analysis was performed in the same manenr as in Example 5, idicating that ethyl 4-chloro-3-hydoxybutyrate with the optical purity of 95.4% ee (S) or more was synthesized in a yield of 98.4%.
EXAMPLE 7
Isolation of β-Ketoacyl-ACP Reductase Gene from Bacillus subtilis 
Bacillus subtilis BGSC 1A1 strain was cultured in the LB medium (containing Bacto-Tryptone 10 g, Bacto-Yeast extract 5 g, and NaCl 10 g/l), and the chromosomal DNA was prepared from the microbial cells thus obtained using a Qiagen Genomic-tip (Qiagen). For the PCR cloning of the β-ketoacyl-ACP reductase gene (fabG) of Bacillus subtilis, the primers BSR-ATG1 (5′-GGACCATGGATATGCTTAATGATAAAACGGCTA-3′, SEQ ID NO: 7) and BSR-TAA1 (5′-GAGAAGCTTCTCGAGTTACATCACCATTCCGCCG-3′, SEQ ID NO: 8) were synthesized based on the sequences at the 5′- and 3′-ends of the structural gene. Using the chromosomal DNA of Bacillus subtilis as the template and the BSR-ATG1 and BSR-TAA1 primers, 30 cycles of PCR (95° C. for 30 sec, 50° C. for 1 min, and 75° C. for 2 min) were performed to obtain the amplified DNA fragments.
The DNA fragments thus obtained were digested with NcoI and HindIII. The plasmid vector pSE420 (Invitrogen) was digested with NcoI and HindIII, and linked to the above PCR-amplified fragments digested with the same two restriction enzymes using T4 DNA ligase to obtain the plasmid pSE-BSR1. The DNA inserts in the plasmid thus obtained were sequenced, identifying them to be the fabG gene except for one amino acid substitution. Namely, GCT corresponding to Ala at the position 25 according to the database (DDBJ (DNA Data Bank of Japan, Accession No. U59433) was replaced by GAT encoding Asp at the position 25. The nucleotide sequence of the fabG gene thus obtained is shown in SEQ ID NO: 6, the amino acid sequence of the protein encoded by the gene in SEQ ID NO: 5.
EXAMPLE 8
Expression of β-Ketoacyl-ACP Reductase Gene derived from Bacillus subtilis 
Escherichia coli HB101 strain was transformed with pSE-BSR1 and the resulting transformant (E. coli HB101 (pSE-BSR1)) was cultured in the LB medium (7 ml) containing ampicillin (50 mg/ml) overnight. IPTG was added to the medium to 0.1 mM and the culture was further incubated for 5 h. Bacterial cells thus obtained were collected, disrupted with a Minibeatbeater 8 (BIOSPEC), and centrifuged to obtain the supernatant as the cell-free extract.
EXAMPLE 9
Reducing Activity of β-Ketoacyl-ACP Reductase Derived from Bacillus subtilis 
The reducing activity of the cell-free extract obtained in Example 8 was assayed using ethyl 4-chloroacetoacetate and acetoacetyl-CoA as the substrate in the same manner as in Example 3.
The results are shown in Table 2. In this table, ECAA represents ethyl 4-chloroacetoacetate and AASCoA acetoacetyl-CoA.











TABLE 2







Substrate
ECAA
AASCoA
S-ECHB
R-ECHB












Coenzyme
NADPH
NADH
NADPH
NADP+
NADP+















U/mg
0.133
0
0.030
0.002
0.002


Relative
100%
0.0%
22.3%
1.3%
1.3%


activity









The cell-free extract expressing β-ketoacyl-ACP reductase showed the NADPH-dependent ethyl 4-chloroacetoacetate-reduce activity and its specific activity was 0.133 U/mg protein. In contrast, the HB101 strain containing no plasmid pSE-BSR1 showed almost no ethyl 4-chloroacetoacetate-reducing activity. The acetoacetyl-CoA-reducing activity was as low as about 22% of ethyl 4-chloroacetoacetate-reducing activity, revealing the difference from the enzyme derived from Escherichia coli. 
EXAMPLE 10
Oxidizing Activity of β-Ketoacyl-ACP Reductase Derived from Bacillus subtilis 
The oxdizing activity of the cell-free extract obtained in Example 8 was assayed using ethyl (S)- or (R)-4-chloro-3-hydroxybutyrate as the substrate in the same manner as in Example 4. The results are shown in Table 2. The cell extract showed almost no activity for either substrate. In table 2, ECHB represents ethyl 4-chloro-3-hydroxybutyrate.
EXAMPLE 11
Stereoselectivity of β-Ketoacyl-ACP Reductase Derived from Bacillus subtilis 
A reaction mixture (1 ml) containing 200 mM potassium phosphate buffer (pH 6.5), 1 mM NADP+, 2% ethyl 4-chloroacetoacetate (122 mM), β-ketoacyl-ACP reductase (0.8 U) prepared in Example 8, 250 mM glucose, and glucose dehyrogenase (3.2 U) (Wako Pure Chemical) was incubated at 25° C. for one day. Analysis was performed by the method according to Example 5, indicating that ethyl 4-chloro-3-hydroxybutyrate was quantitatively synthesized with the optical purity of 98.1% ee (S) or more.
EXAMPLE 12
Isolation of Acetoacetyl-CoA Reductase Gene from Ralstonia eutropha 
Ralstonia eutropha DSM 531 was cultured in a bouillon medium (containing beaf extracts 5.0 g, peptone 15.0 g, NaCl 5.0 g, and K2HPO4 5.0 g/l) and the chromosomal DNA was prepared from the microbial cells thus obtained using a Qiagen Genomic-tip (Qiagen).
For the PCR cloning of the acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha, the primers AER-ATG1 (5′-AGTGGATCCAATGACTCAGCGCATTGCGTA-3′, SEQ ID NO: 11) and AER-TAA1 (5′-AACAAGCTTCTCGAGTTAGCCCATATGCAGGCCGC-3′, SEQ ID NO: 12) were synthesized based on the sequences at the 5′- and 31-ends of the structural gene.
Using the chromosomal DNA of Ralstonia eutropha as the template and the AER-ATG1 and AER-TAA1 primers, 30 cycles of PCR (95° C. for 30 sec, 50° C. for 1 min and 75° C. for 2 min) were performed to obtain the amplified DNA fragments.
The thus-obtained DNA fragments were digested with BamHI and HindIII. The plasmid vector pSE420 prepared by the method of Example 1 was digested with BamHI and HindIII and the digestion product was ligated to the above PCR-amplified fragments digested with the same two restriction enzymes using T4 DNA ligase to obtain the plasmid pSE-AER1.
The DNA insert in the plasmid thus obtained were sequenced and identified as the phbb gene.
EXAMPLE 13
Expression of Acetoacetyl-CoA Reductase Gene Derived from Ralstonia eutropha 
Escherichia coli HB101 strain was transformed with pSE-AER1 and the resulting transformant (E. coli HB101 (pSE-AER1)) was cultured in the LB medium (7 ml) containing ampicillin (50 mg/ml) overnight. IPTG was added to the medium to 0.1 mM and the culture was further incubated for 5 h.
Bacterial cells thus obtained were collected, disrupted with a Minibeatbeater 8 (BIOSPEC Inc.), and centrifuged to obtain the supernatant as the cell-free extract.
EXAMPLE 14
Reducing Activity of Acetoacetyl-CoA Reductase Derived from Ralstonia eutropha 
The reducing activity of the cell-free extract obtained in Example 13 was assayed using ethyl 4-chloroacetoacetate and acetoacetyl-CoA as the substrate by the method according to Example 3. The results are shown in Table 3.











TABLE 3







Substrate
AASCoA
ECAA
S-ECHB
R-ECHB












Coenzyme
NADPH
NADPH
NADH
NADP+
NADP+















U/mg
0.984
2.50
0.056
0 
0 


Relative
100%
255%
5.7%
0%
0%


activity









The cell-free extract expressing acetoacyl-CoA reductase showed the NADPH-dependent ethyl 4-chloroacetoacetate-reducing activity and its specific activity was 2.50 U/mg protein. In contrast, the HB101 strain containing no plasmid pSE-AER1 showed almost no ethyl 4-chloroacetoacetate-reducing activity.
The reducing activity with acetoacetyl-CoA was as low as about 39% of that with ethyl 4-chloroacetoacetate.
EXAMPLE 15
Oxidizing Activity of Acetoacetyl-CoA Reductase Derived from Ralstonia eutropha 
The oxdizing activity of the cell-free extract obtained in Example 13 was assayed using ethyl (S)- or (R)-4-chloro-3-hydroxybutyrate as the substrate in the same manner as in Example 4. The results are shown in Table 3. The cell extract showed almost no oxidizing activity for either substrate.
EXAMPLE 16
Stereoselectivity of Acetoacetyl-CoA Reductase from Ralstonia eutropha 
A reaction mixture (1 ml) containing 100 mM potassium phosphate buffer (pH 6.5), 1 mM NADP+, 2% (122 mM) ethyl 4-chloroacetoacetate, acetoacyl-CoA reductase (1 U) prepared in Example 13, 243 mM glucose, and glucose dehyrogenase (2.8 U) (Wako Pure Chemical) was incubated at 25° C. overnight. Analysis was performed by the method according to Example 5, revealing a synthesis of ethyl 4-chloro-3-hydroxybutyrate with the optical purity of 99% ee (S) or more in a yield of 67%.
EXAMPLE 17
Construction of the Coexpression Plasmid pSE420D
The plasmid pSE420B constructed in Example 1 was digested with MunI and SpeI and ligated with the annealing product of the synthetic DNA SE420D-S (SEQ ID NO 13: AATTCTCGAGTAATCTAGAGGAATTCTAAAA) and the synthetic DNA SE420D-A (SEQ ID NO 14: CTAGTTTTAGAATTCCTCTAGATTACTCGAG) using T4 DNA ligase to obtain the plasmid pSE420D. The restriction map of pSE420D is shown in FIG. 1.
EXAMPLE 18
Cloning of Glucose Dehydrogenase Gene Derived from Bacillus subtilis 
In order to regenerate the reduced nicotinamide adenine dinucleotide phosphate, the glucose dehydrogenase gene derived from Bacillus subtilis (J. Bateriol. 166, 238-243 (1986)) was cloned.
For the PCR cloning of only the open reading frame portion of the glucose dehydrogenase gene based on the nucleotide sequence described in the literature, the primers BSG-ATG1 (SEQ ID NO 15: GAGGAATTCATACATGTATCCAGATTTAAAAGGAA) and BSG-TAA2 (SEQ ID NO 16: GGTAAGCTTTCATTAACCGCGGCCTGCCTG) were synthesized based on the sequences at the 5′- and 3′-ends of the structural gene.
Using the chromosomal DNA of Bacillus subtilis prepared in Example 7 as the template and the BSG-ATG1 and BSG-TAA2 primers, 30 cycles of PCR (95° C. for 30 sec, 50° C. for 1 min, and 75° C. for 3 min and 15 sec) were performed, obtaining the amplified DNA fragments.
The thus-obtained DNA fragments were digested with EcoRI and HindIII and the digestion product was ligated with the plasmid vector pSE420L constructed in Example 17 digested with the same two restriction enzymes using T4 DNA ligase to obtain the plasmid pSE-BSG1.
Analysis of the nucleotide sequence of the DNA insert resulted in the perfect coincidence with that listed in the database (DDBJ Accession No. M12276).
The nucleotide sequence of the glucose dehydrogenase gene thus obtained is shown in SEQ ID NO: 17 and the amino acid sequence of the protein encoded by the gene in SEQ ID NO: 18.
EXAMPLE 19
Construction of the Plasmid pSG-ECR1 Coexpressing the β-Ketoacyl-ACP Reductase Gene Derived from Escherichia coil and the Glucose Dehydrogenase Gene Derived from Bacillus subtilis 
The plasmid pSE-ECR1 constructed in Example 1 was digested with BamHI and XhoI to obtain the DNA fragment containing the β-ketoacyl-ACP reductase gene derived from Escberichia coli. 
The plasmid pSE-BSG1 containing the glucose dehydrogenase gene derived from Bacillus subtilis constructed in Example 17 was digested with BamHI and XhoI, ligated to the above DNA fragment containing the β-ketoacyl-ACP reductase gene derived from Escherichia coli using T4 DNA ligase to obtain the plasmid pSE-ECR1 capable of coexpressing glucose dehydrogenase and β-ketoacyl-ACP reductase. The restriction map of the plasmid pSE-ECR1 is shown in FIG. 2.
EXAMPLE 20
Construction of the Plasmid pSG-ECR1 Coexpressing the β-Ketoacyl-ACP Reductase Gene Derived from Bacillus subtilis and the Glucose Dehydrogenase Gene Derived from Bacillus subtilis 
The plasmid pSE-ECR1 constructed in Example 7 was digested with NcoI and XhoI to obtain the DNA fragment containing the β-ketoacyl-ACP reductase gene derived from Bacillus subtilis. 
The plasmid pSE-BSG1 containing the glucose dehydrogenase gene derived from Bacillus subtilis constructed in Example 17 was digested with BamHI and XhoI, ligated to the above DNA fragment containing the β-ketoacyl-ACP reductase gene derived from Bacillus subtilis using T4 DNA ligase to obtain the plasmid pSG-BSR1 capable of coexpressing glucose dehydrogenase and β-ketoacyl-ACP reductase. The restriction map of the plasmid pSG-BSR1 is shown in FIG. 3.
EXAMPLE 21
Construction of Plasmid pSG-AER1 Coexpressing the Acetoacetyl-CoA Reductase Gene Derived from Ralstonia eutropha and the Glucose Dehydrogenase Gene Derived from Bacillus subtilis 
The plasmid pSE-AER1 constructed in Example 12 was digested with BamHI and XhoI to obtain the DNA fragment containing the acetoacetyl-CoA reductase gene derived from Ralstonia eutropha. 
The plasmid pSE-BSG1 containing the glucose dehydrogenase gene derived from Bacillus subtilis constructed in Example 17 was digested with NcoI and XhoI, ligated to the above DNA fragment containing the acetoacetyl-CoA reductase gene derived from Ralstonia eutropha using T4 DNA ligase to obtain the plasmid pSG-AER1 capable of coexpressing glucose dehydrogenase and acetoacetyl-CoA reductase. The restriction map of the plasmid pSG-AER1 is shown in FIG. 4.
EXAMPLE 22
Coexpression of Glucose Dehydrogenase Derived from Bacillus subtilis and β-ketoacyl-ACP Reductase Derived from Escherichia coli or β-Ketoacyl-ACP Reductase Derived from Bacillus subtilis or Acetoacetyl-CoA Reductase Derived from Ralstonia eutropha 
Escherichia coli HB101 strain was transformed with the plasmids pSG-ECR1, PSG-BSR1, or pSG-AER1 to coexpress glucose dehydrogenase derived from Bacillus subtilis with β-ketoacyl-ACP reductase derived from Escherichia coli or with β-ketoacyl-ACP reductase derived from Bacillus subtilis or with acetoacetyl-CoA reductase derived from Ralstonia eutropha. 
Recombinant Escherichia coli transformed with each plasmid was inoculated to the 2×YT medium (containing Bacto-Triptone 20 g, a Bacto-Yeast extract 10 g, and NaCl 10 g/l), cultured at 30° C. overnight. Adding 0.1 mM IPTG to the medium, the culturing was further performed for 4 h.
Three kinds of Escherichia coli transformants thus obtained were collected and subjected to the enzyme activity assay and the reducing reaction of ethyl 4-chloroacetoacetate.
EXAMPLE 23
Enzymatic Activity of Escherichia coli Cells Transformed with pSG-ECR1, pSG-BSR1 and pSG-AER1
Escherichia coli cells (corresponding to 2 ml of the culture) transformed with pSG-ECR1, pSG-BSR1, or pSG-AER1 prepared in Example 21 were separately suspended in 0.25 ml of a cell disrupting solution (containing 100 mM potassium phosphate buffer (pH 6.5), 0.02% 2-mercaptoethanol, and 0.5 M sodium chloride). The resulting solution was treated in a close ultrasonicator UCD-200TM (Cosmobio) for 3 min to disrupt the cells. The sonicated cell suspension was centrifuged and the supernatant was collected as the crude enzyme solution, which was subjected to the enzyme activity assay.
The ethyl 4-chloroacetoacetate-reducing activity was assayed in the same manner as in Example 3.
Glucose dehydrogenase activity was assayed in a reaction solution containing 100 mM potassium phosphate bufferr (pH 6.5), 2.5 mM NAD+, 100 mM D-glucose, and the enzyme at 25° C.
One unit of each enzyme was defined as the amount of the enzyme catalyzing the formation of 1 μmol NADH per min under the above-described reaction conditions.
Each enzyme activity and SECHB productivity of the crude enzyme t solution obtained from recombinant E. coli containing pSG-ECR1, pSG-BSRI1, or pSG-AER1 are shown in Table 4.












TABLE 4









Enzyme activity

SECHB




(U/ml-br)

synthesis













Plasmid
GlcDH
ECAA-R
(g/l)
ee (%) S







pSG-ECR1
0.476
0.151
20.3
93.7



pSG-BSR1
4.089
0.203
42.3
98.0



pSG-AER1
3.717
1.475
42.8
99.3







U/ml-br: enzyme activity per ml of the culture 



GlcDH: glucose dehydrogenase 



ECAA-R: ethyl 4-chloroacetoacetate reductase 



SECHB: ethyl (S)-4-chloro-3-hydroxybutyrate 






E. coli cells with no plasmid have neither glucose dehydrogenase activity nor ethyl 4-chloroacetoacetate reductase activity, while recombinant E. coli cells with pSG-ECR1, pSG-BSR1, or pSG-AER1 showed both enzymatic activities, indicating expression of the two enzyme genes introduced in the plasmids.
EXAMPLE 24
Synthesis of Ethyl (S)-4-chloro-3-hydroxybutyrate Using Escherichia coli Transformed with pSG-ECR1, pSG-BSR1, or pSG-AER1
Ethyl 4-chloroacetoacetate was reduced using Escherichia coli containing pSG-ECR1, pSG-BSR1, or pSG-AER1 prepared in Example 21.
A reaction solution (20 ml) containing each Escherichia coli cell prepared from 20 ml of the culture, 200 mM potassium phosphate buffer (pH 6.5), 4% ethyl 4-chloroacetoacetate, 486 mM D-glucose, and 1 mM NADP+ was incubated at 25° C. under stirring overnight.
The amount and the optical purity of ethyl (S)-4-chloro-3-hydroxybutyrate were determined in the same manner as in Example 5. The results are shown in Table 4. As shown in Table 4, all of the three kinds of the E. coli transformants could produce (S)-4-chloro-3-hydroxybutyrate efficiently with high sterepselectivity.

## Claims

1. A method for producing a (S)-4-halo-3-hydroxybutyric acid ester, the method comprising asymmetrically reducing a 4-haloacetoacetic acid ester or its derivative with a purified acetoacetyl-CoA reductase (EC 1.1.1.36) that participates in a poly-β-hydroxy fatty acid biosynthesis system.

2. The method of claim 1, wherein said acetoacetyl-CoA reductase is from a microorganism belonging to the genus Ralstonia.

3. The method of claim 2, wherein the microorganism is Ralstonia eutropha.

4. The method of claim 1, wherein said acetoacetyl-CoA reductase comprises the amino acid sequence of SEQ ID NO:9.

5. The method of claim 1, wherein said 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester.

6. The method of claim 1, wherein said 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate.

7. The method of claim 1, wherein said acetoacetyl-CoA reductase is from Zoogloe ramigera.

8. The method of claim 1, wherein the reduction is carried out in the presence of glucose dehydrogenase.

9. The method of claim 2, wherein the reduction is carried out in the presence of glucose dehydrogenase.

10. The method of claim 3, wherein the reduction is carried out in the presence of glucose dehydrogenase.

11. The method of claim 4, wherein the reduction is carried out in the presence of glucose dehydrogenase.

12. The method of claim 5, wherein the reduction is carried out in the presence of glucose dehydrogenase.

13. The method of claim 6, wherein the reduction is carried out in the presence of glucose dehydrogenase.

14. The method of claim 7, wherein the reduction is carried out in the presence of glucose dehydrogenase.

15. The method of claim 2, wherein the 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester.

16. The method of claim 2, wherein the 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate.

17. The method of claim 3, wherein the 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester.

18. The method of claim 3, wherein the 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate.

19. The method of claim 4, wherein the 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester.

20. The method of claim 4, wherein the 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate.

21. The method of claim 7, wherein the 4-haloacetoacetic acid ester is 4-chloroacetoacetic acid ester.

22. The method of claim 7, wherein the 4-haloacetoacetic acid ester is ethyl 4-chloroacetoacetate.