APPENDIX B. T-REX Example Output 

Sample output for foliar spray applications of endosulfan based on based 1 application to citrus 

(non bearing) at a single application rate of 2.5 lb a.i./A (Maximum EEC Scenario). 

Summary of Risk Quotient Calculations Based on Upper Bound Kenaga EECs 

Table B-1. Upper Bound Kenaga, Acute Avian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore Size 

Class 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

20 14.54 683.34 47.00 313.20 21.54 384.38 26.44 42.71 2.94 9.49 0.65 

100 18.51 389.67 21.05 178.60 9.65 219.19 11.84 24.35 1.32 5.41 0.29 

1000 26.14 174.46 6.67 79.96 3.06 98.13 3.75 10.90 0.42 2.42 0.09 

 

Table B-2. Upper Bound Kenaga, Subacute Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 

805 600.00 0.75 275.00 0.34 337.50 0.42 37.50 0.05 

Size class not used for dietary risk quotients 

B-1
Table B-3. Upper Bound Kenaga, Chronic Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

NOAEC 

(ppm) EEC RQ EEC RQ EEC RQ EEC RQ 

30 600.00 20.00 275.00 9.17 337.50 11.25 37.50 1.25 

Size class not used for dietary risk quotients 

 

Table B-4. Upper Bound Kenaga, Acute Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size 

Class 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 21.98 572.05 26.03 262.19 11.93 321.78 14.64 35.75 1.63 7.95 0.36 

35 17.78 395.37 22.23 181.21 10.19 222.39 12.51 24.71 1.39 5.49 0.31 

1000 7.69 91.67 11.92 42.01 5.46 51.56 6.70 5.73 0.74 1.27 0.17 

 

Table B-5. Upper Bound Kenaga, Chronic Mammalian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

NOAEC 

(ppm) 

EEC RQ EEC RQ EEC RQ EEC RQ 

15 600.00 40.00 275.00 18.33 337.50 22.50 37.50 2.50 

Size class not used for dietary risk quotients 

Table B-6. Upper Bound Kenaga, Chronic Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size 

Class 

(grams) 

Adjusted 

NOAEL 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 1.65 572.05 347.04 262.19 159.06 321.78 195.21 35.75 21.69 7.95 4.82 

35 1.33 395.37 296.44 181.21 135.87 222.39 166.75 24.71 18.53 5.49 4.12 

1000 0.58 91.67 158.90 42.01 72.83 51.56 89.38 5.73 9.93 1.27 2.21 

B-2
Terrestrial Residues (upper bound estimates)

vs

Avian LOCs (as dietary concentrations)

0.00

100.00

200.00

300.00

400.00

500.00

600.00

700.00

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104

Days

Concentration (mg ai/kg dietary item)

Short Grass Tall Grass Broadleaf plants/sm Insects Fruits/pods/seeds/lg insects

Avian Acute Non-endangered LOC Avian Chronic LOC Avian Acute Endangered LOC

Terrestrial Residues (upper bound estimates)

vs

Mammalian Herbivore/Insectivore LOCs (as dietary concentrations

0.00

100.00

200.00

300.00

400.00

500.00

600.00

700.00

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104

Days

Concentration (mg ai/kg dietary item)

Short Grass Tall Grass Broadleaf plants/sm Insects Fruits/pods/seeds/lg insects

Chronic LOC 15 g Mammal Acute LOC 35 g Mammal Acute LOC 1000 g Mammal Acute LOC

15 g Endangered Mammal Acute LOC 35 g Endangered Mammal Acute LOC 1000 g Endangered Mammal Acute LOC

Figure B-1. Time course of predicted concentrations of endosulfan on terrestrial forage and prey items in 

relation to levels of concern for birds (top panel) and mammals (bottom panel) using T-REX 1.4.1; Citrus nb. 

B-3
Sample output for foliar spray applications of endosulfan based on based 1 application to CA 

Eggplant at a single application rate of 0.5 lb a.i./A (Minimum EEC Scenario). 

Summary of Risk Quotient Calculations Based on Upper Bound Kenaga EECs 

Table B-7. Upper Bound Kenaga, Acute Avian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size 

Class 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

20 14.54 136.67 9.40 62.64 4.31 76.88 5.29 8.54 0.59 1.90 0.13 

100 18.51 77.93 4.21 35.72 1.93 43.84 2.37 4.87 0.26 1.08 0.06 

1000 26.14 34.89 1.33 15.99 0.61 19.63 0.75 2.18 0.08 0.48 0.02 

 

Table B-8. Upper Bound Kenaga, Subacute Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 

805 120.00 0.15 55.00 0.07 67.50 0.08 7.50 0.01 

Size class not used for dietary risk quotients 

 

Table B-9. Upper Bound Kenaga, Chronic Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects NOAEC 

(ppm) EEC RQ EEC RQ EEC RQ EEC RQ 

30 120.00 4.00 55.00 1.83 67.50 2.25 7.50 0.25 

Size class not used for dietary risk quotients 

 

Table B-10. Upper Bound Kenaga, Acute Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size 

Class 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 21.98 114.41 5.21 52.44 2.39 64.36 2.93 7.15 0.33 1.59 0.07 

35 17.78 79.07 4.45 36.24 2.04 44.48 2.50 4.94 0.28 1.10 0.06 

1000 7.69 18.33 2.38 8.40 1.09 10.31 1.34 1.15 0.15 0.25 0.03 

 

 

B-4
Table B-11. Upper Bound Kenaga, Chronic Mammalian Dietary Based Risk 

Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

NOAEC 

(ppm) 

EEC RQ EEC RQ EEC RQ EEC RQ 

15 120.00 8.00 55.00 3.67 67.50 4.50 7.50 0.50 

Size class not used for dietary risk quotients 

 

Table B-12. Upper Bound Kenaga, Chronic Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size 

Class 

(grams) 

Adjusted 

NOAEL 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 1.65 114.41 69.41 52.44 31.81 64.36 39.04 7.15 4.34 1.59 0.96 

35 1.33 79.07 59.29 36.24 27.17 44.48 33.35 4.94 3.71 1.10 0.82 

1000 0.58 18.33 31.78 8.40 14.57 10.31 17.88 1.15 1.99 0.25 0.44 

B-5
Terrestrial Residues (upper bound estimates)

vs

Avian LOCs (as dietary concentrations)

0.00

50.00

100.00

150.00

200.00

250.00

300.00

350.00

400.00

450.00

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104

Days

Concentration (mg ai/kg dietary item)

Short Grass Tall Grass Broadleaf plants/sm Insects Fruits/pods/seeds/lg insects

Avian Acute Non-endangered LOC Avian Chronic LOC Avian Acute Endangered LOC

Terrestrial Residues (upper bound estimates)

vs

Mammalian Herbivore/Insectivore LOCs (as dietary concentrations

0.00

20.00

40.00

60.00

80.00

100.00

120.00

140.00

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104

Days

Concentration (mg ai/kg dietary item)

Short Grass Tall Grass Broadleaf plants/sm Insects Fruits/pods/seeds/lg insects

Chronic LOC 15 g Mammal Acute LOC 35 g Mammal Acute LOC 1000 g Mammal Acute LOC

15 g Endangered Mammal Acute LOC 35 g Endangered Mammal Acute LOC 1000 g Endangered Mammal Acute LOC

Figure B-2. Time course of predicted concentrations of endosulfan on terrestrial forage and prey items in 

relation to levels of concern for birds (top panel) and mammals (bottom panel) using T-REX 1.4.1; CA 

Eggplant. 

B-6