# Sampling and Analysis Plan (SAP) & Quality Assurance Project Plan (QAPP)

For

Jacksonville ODMDS Fall 2010 Site Designation Survey

Contract # W912EP-09-C-0058

Submitted to
US Army Corps of Engineers—Jacksonville District 701 San Marco Boulevard Jacksonville, Florida 32207

And

US Environmental Protection Agency, Region 4 Sam Nunn Atlanta Federal Center 61 Forsyth Street, SW Atlanta, Georgia 30303

Submitted by
ANAMAR Environmental Consulting, Inc. 2106 NW 67 th Place, Suite 5 Gainesville, Florida 32653-1658

September 2010Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 1.0, Element A1: Title and Approval Sheet
2 

## GROUP A. PROJECT MANAGEMENT

1.0 ELEMENT A1 - TITLE AND APPROVAL SHEET

Title: Jacksonville Ocean Dredged Material Disposal Site (ODMDS) Site Designation Studies

Organization/Applicant: U.S. Army Corps of Engineers—Jacksonville District Project Officer: April Patterson Signature: Date:

QA/QC Officer: Glenn Schuster Signature: Date:

Regulatory Agency: USEPA, Region 4 Designated Approving Official: Chris McArthur Signature: Date:

Technical Review: Chris McArthur Signature: Date:

Contractor: ANAMAR Environmental Consulting, Inc. Project Manager: Michelle Rau Signature: Date:

QA/QC Officer: Paul Berman Signature: Date:Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Appendix 3 2.0 ELEMENT A2 - TABLE OF CONTENTS

TABLE OF CONTENTS

1.0 ELEMENT A1 - TITLE AND APPROVAL SHEET

.................................................................................. 2
2.0 ELEMENT A2 - TABLE OF CONTENTS

.................................................................................................... 3
3.0 ELEMENT A3 - DISTRIBUTION LIST

.................................................................................................... 8
4.0 ELEMENT A4 - PROJECT/TASK ORGANIZATION

............................................................................ 9 4.1 Survey Project Proponent

............................................................................................................ 9 4.2 Survey Project Team and Responsibilities

............................................................................ 9
5.0 ELEMENT A5 - PROBLEM DEFINITION/BACKGROUND

............................................................. 12 5.1 Purpose and Need/Site History

............................................................................................... 14 5.2 Identification of Principal Data Users and Decision Makers

....................................... 18
6.0 ELEMENT A6 - TASK DESCRIPTION

................................................................................................... 19 6.1 Purpose/Background

.................................................................................................................. 19 6.2 Description of the Sampling and Analysis

.......................................................................... 22 6.2.1. Measurements That Are Expected Du ring the Course of the Sampling Survey

............................................................................................................................ 22 6.2.2. Applicable Technical Quality Standards or Criteria

..................................... 24 6.2.3. Special Personnel or Equipment Re quirements That May Indicate the Complexity of the Dredging Project

........................................................... 25 6.2.4. Assessment Techniques Needed for the Dredging Project

....................... 25 6.2.5. Schedule for the Work Performed

...................................................................... 26 6.2.6. Required Deliverables/Reports

........................................................................... 27
7.0 ELEMENT A7 - QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT DATA

......... 29
8.0 ELEMENT A8 - SPECIAL TRAINING REQUIREMENTS/CERTIFICATION

.............................. 32
9.0 ELEMENT A9 - DOCUMENTATION AND RECORDS

......................................................................... 33 9.1 Reporting of Results

..................................................................................................................... 33 9.2 Report Format

............................................................................................................................... .. 34 Data Reporting Package Archiving and Retrieval

........................................................... 36
10.0 ELEMENT B1 - SAMPLING PROCESS DESIGN

................................................................................ 37 10.1 Scheduled Project Activities, In cluding Measurement Activities

.............................. 37 10.2 Rationale for the Design

............................................................................................................ 37 10.3 Design Assumptions

.................................................................................................................... 37 10.4 Procedures for Locating and Se lecting Environmental Samples

............................... 38 10.5 Classification of Measuremen ts as Critical or Noncritical

............................................ 44 10.6 Validation of Any Nonstandard Methods

............................................................................. 44
11.0 ELEMENT B2 – SAMPLING AND METHODS REQUIREMENTS

................................................... 45 11.1 Describe the Sample Collection, Preparation, and Decontamination Procedures 45 11.2 Identify Support Facilitie s for Sampling Methods

........................................................... 51 11.3 Describe Sampling/Measurement System Failure Response and Corrective Action Process

.......................................................................................................... 52 11.4 Describe Sampling Equipment, Samp le Preservation, and Holding Times

............ 52
12.0 ELEMENT B3 – SAMPLE HANDLING AND CUSTODY REQUIREMENTS

.................................. 55
13.0 ELEMENT B4 – ANALYTICAL METHODS REQUIREMENTS

......................................................... 57 13.1 Subsampling

.............................................................................................................................. ..... 57 13.2 Preparation of the Samples

...................................................................................................... 57Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Appendix 4 13.3 Analytical Methods

....................................................................................................................... 57 13.3.1 Physical and Chemical Analysis

........................................................................... 57 13.3.2 Taxonomic Analysis

.................................................................................................. 61
14.0 ELEMENT B5 - QUALITY CONTROL REQUIREMENTS

.................................................................. 64
15.0 ELEMENT B6 – INSTRUMENT/EQUIPMENT TESTING, INSPECTION, AND MAINTENANCE REQUIREMENTS

........................................................................................................ 67
16.0 ELEMENT B7 – INSTRUMENT CALIBRATION AND FREQUENCY

............................................. 68
17.0 ELEMENT B8 – INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPLIES AND CONSUMABLES

.............................................................................................................................. ............ 69
18.0 ELEMENT B9 – DATA ACQUISITION REQUIREMENTS (NON-DIRECT MEASUREMENTS) 70
19.0 ELEMENT B10 – DATA MANAGEMENT, INTERPRETATION, AND REDUCTION

.................. 71
20.0 ELEMENT C1 – ASSESSMENTS AND RESPONSE ACTIONS

........................................................ 72
21.0 ELEMENT C2 – REPORTS TO MANAGEMENT

.................................................................................. 73
22.0 ELEMENT D1 – DATA REVIEW, VALIDATION, AND VERIFICATION REQUIREMENTS

... 75
23.0 ELEMENT D2 – VALIDATION AND VERIFICATIONS METHODS

.............................................. 76
24.0 ELEMENT D3 – RECONCILIATION WITH DATA QUALITY OBJECTIVES

............................... 77
25.0 REFERENCES

.............................................................................................................................. ................ 78Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Appendix 5 List of Figures Figure 4-1 Project Organization Chart Figure 5-1 Dredging Area and ODMDS Figure 5-2 Jacksonville Harbor Navigation Project Figure 6-1a Proposed Sampling Stations in and around Candidate Sites 1 and 2 Figure 6-1b Proposed Sampling Statio ns in and around Candidate Site 3

List of Tables Table 5-1 Five General and Eleven Specific Site Selection Criteria Table 5-2 Projected 10-Year Stat us of the Jacksonville ODMDS Table 5-3 Areas of Responsibility for Principle Data Users Table 6-1 Fall 2010 Field Sampling and Analysis Scheme for Jacksonville ODMDS Site Designation Sampling Event
Table 6-2 Estimated Schedule of Work Performed Table 7-1 Data Quality Objectives for Water and Tissue Analyses Table 7-2 Data Quality Objectives for Enumeration and Taxonomy of Invertebrates and Fishes Table 9-1 Laboratories Submitting Data for this Project Table 10-1 Infaunal Sample Identification and Station Coordinates Table 10-2 Clam Dredge Sample Station Identification and Station Coordinates Table 10-3 Epifaunal Samp le Station Identification and Station Coordinates Table 10-4 In Situ (CTD-profiler) and Water Chemistry Station Identification and Station Coordinates Table 10-5 Sample Analysis Table 11-1 Standard Operating Procedures Used During Sampling Table 11-2 Minimum Volume Requ irements per Sample for Physical and Chemical Analysis Table 11-3 Container Requirements for Water Samples for Chemical Analysis Table 11-4 Container Requirements for Tissue Samples for Chemical Analysis Table 11-5 Container Requirements for Infaunal Samples for Taxonomic Analysis Table 11-6 Container Requirements for Epifa unal Samples for Identity Verification and Possible Vouchering Table 11-7 Container Requirements for Clam Dredge Samples for Identity Verification and Possible Vouchering Table 12-1 Field Activity Forms Used to Document Sample Collection and Support Activities Table 13-1 Analytes, Methods, and Target Reporting Limits: Water Chemistry Table 13-2 Analytes, Methods, and Target Reporting Limits: Tissue Chemistry Table 14-1 Quality Control Activities Performe d for Field Activities and Chemical Analyses Table 21-1 Minimum Reporting Requirements
Table 21-2 Electronic Raw Data File FormatSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Appendix 6 Appendices Sediment Sampling Field Log Water Chemistry Sampling Field Log Infaunal Sampling Log Clam Dredge Sampling Log Epifaunal (Trawl) Sampling Log Chain-of-Custody Form Daily Quality Control Report Temperature LogSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 2.0, Element A2: Table of Contents
7 List of Acronyms

CCL contaminant cleanup target levels CQAR Chemical Quality Assurance Report cy cubic yards DQCR Daily Quality Control Report ERL effects range-low FDEP Florida Department of Environmental Protection FLMNH Florida Museum of Natural History (University of Florida)
MDL method detection limit RL reporting limit NELAC National Environmental Laboratory Association Conference ODMDS Ocean Dredged Material Disposal Site PAH polynuclear aromatic hydrocarbons (also known as polycyclic aromatic hydrocarbons) PCB polychlorinated biphenyls QA/QC quality assurance/quality control QAM Quality Assurance Manual QAPP Quality Assura nce Project Plan SAP Sampling and Analysis Plan SMMP site management and monitoring plan
TBD to be determined
TEL threshold effects level TOC total organic carbon TSS total suspended solids USACE U.S. Army Corps of Engineers USEPA U.S. Environmental Protection AgencySampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 3.0, Element A3: Distributi on List Project/Task Organization
8 3.0 ELEMENT A3 - DISTRIBUTION LIST
This document is to be distributed to the following individuals for review and approval prior to commencement of sampling activities. Additional electronic copies are available by request from ANAMAR Environmental Consulting, Inc.

1. USACE Project Officer: April Patterson

2. USACE QA/QC Manager: Glenn Schuster

3. USEPA Designated Approving Official: Chris McArthur

4. USEPA Technical Review: Chris McArthur

5. ANAMAR Project Manage r: Michelle Rau

6. ANAMAR QA/QC Officer: Paul BermanSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 4.0, Element A4: Project/Task Organization
9 4.0 ELEMENT A4 - PROJECT/TASK ORGANIZATION

4.1 Survey Project Proponent Proponent: US Army Corps of Engineers—Jacksonville District Regulatory: US Environmental Protection Agency, Region 4

4.2 Survey Project Team and Responsibilities Organization: US Army Corps of Engineers—Jacksonville District Project Officer: April N. Patterson 701 San Marco Boulevard Jacksonville, Florida 32207 Phone: (904) 232-2610 Mobile: (904) 502-5325 E-mail: april.n.patterson@usace.army.mil Responsibilities: Project Office r for the Jacksonville ODMDS Site Designation Studies. Also responsible for co-management of the Jacksonville ODMDS once approved for use by USEPA.

Organization: US Army Corps of Engineers—Jacksonville District QA/QC Manager: Glenn R. Schuster 701 San Marco Boulevard Jacksonville, Florida 32207 Phone: (904) 232-3691 Fax: (904) 232-1904 E-mail: glenn.r.schuster@saj02.usace.army.mil Responsibility: QA/QC for the Jacksonv ille ODMDS Site Designation Studies.

Organization: US Environmental Protection Agency, Region 4 Designated Approving Official/Technical Reviewer: Chris McArthur Wetlands and Marine Regulatory Section 61 Forsyth Street, SW Atlanta, Georgia 30303 Phone: (404) 562-9391 Fax: (404) 562-9343 E-mail: mcarthur.chris topher@epamail.epa.gov Responsibilities: Conduct designation surv ey of the proposed Jacksonville ODMDS in accordance with 40 CFR 228.6(b) and approve or deny use based on survey results. Also responsible for co-management of the Jacksonville ODMDS with USACE.

Contractor 1: ANAMAR Environmental Consulting, Inc. Project Manager: Michelle Rau 2106 NW 67 th Place, Suite 5 Gainesville, Florida 32653-1658 Phone: (352) 377-5770 ext. 107 Fax: (352) 378-7620 E-mail: mrau@anamarinc.com Responsibilities: Project ma nagement, conduct designation survey, sample handling and transport, chains of custody, quality a ssurance management, final data reportingSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 4.0, Element A4: Project/Task Organization
10 Contracted by: USACE—Jacksonville Distri ct under Contract # W912EP-09-R-0054

Chemistry Laboratory 1: Columbia Analytical Services, Inc. (CAS) Project Manager: Jeff Christian 1317 S. 13th Avenue Kelso, Washington 98626 Phone: (360) 501-3316 Fax: (360) 636-1068 E-mail: jchristian @kelso.caslab.com Responsibilities: Sample holding and archivin g, laboratory preparation and analysis for sediment and site water. Contracted by: ANAMAR Environmental Consulting, Inc.

Geotechnical Laboratory: MACTEC/Law Engineering Project Manager: Mark Coleman 3901 Carmichael Avenue Jacksonville, Florida 32207 Phone: (904) 396-5173 Fax: (904) 396-5703 E-mail: macoleman@mactec.com Responsibilities: Sample holding and archiving, laboratory preparation an d analysis for physical analyses. Contracted by: ANAMAR Environmental Consulting, Inc.

Taxonomic Laboratory: Barry A. Vittor & Associates, Inc. Project Manager: Carl M. Way 8060 Cottage Hill Rd. Mobile, Alabama 36695 Phone: (251) 633-6100 Mobile: (252) 510-1831 E-mail: cway@bvaenviro.com Responsibilities: Infaunal identification and analysis. Contracted by: ANAMAR Environmental Consulting, Inc.

The successful completion of this project relies on open lines of communication between the client, contractor, regulatory agencies, laboratories, and subcontractors. This communication and successful completion of the project must be the contractor’s utmost goal. Contact information will be readily available throughout the life of this project, from pre-planning to field work, data analysis, data reduction, and reportin g. Any questions, clarifications, suggestions, and/or problems will be addressed in a timely manner.

See Project Organization Chart on next page.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 4.0, Element A4: Project/Task Organization
11 

### Figure 4-1. Project Organization Chart

USACE Project Officer: April Patterson (904) 232-3691 QA/QC Manager: Glenn Schuster (904)232-3691

Geotechnical Lab: MACTEC Project Manager:

Mark Coleman (904) 396-5173 Chemistry Lab: CAS Project Manager:

Jeff Christian (360) 501-3316 USEPA Designated Approving Official/Technical Reviewer: Chris McArthur (404) 562-9391

Contractor: ANAMAR QA/QC Manager: Paul Berman (352) 377-5770 x106 Contractor: ANAMAR Project Manager: Michelle Rau (352) 377-5770 x107

Infaunal Taxonomic Lab: Barry Vittor & Assoc. Project Manager: Carl M. Way (251) 633-6100Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
12 5.0 ELEMENT A5 - PROBLEM DEFINITION/BACKGROUND The U.S. Environmental Protection Agency, Region 4 (USEPA) and U.S. Army Corps of Engineers—Jacksonville District (USACE) share re sponsibility for the cont rol and management of ocean disposal of dredged material under the Marine Protection, Research, and Sanctuaries Act of 1972 (33 U.S.C. 1401 et seq., MPRSA). There are three distinct but interrelated activities for which USEPA and/or USACE have responsibilitie s with respect to ocean disposal of dredged material (USEPA and USACE 2007): 1) Designation or selection of sites fo r ocean disposal of dredged material, 2) Evaluation of the suit ability of dredged material for ocean disposal, and 3) Management and monitoring of Ocean Dr edged Material Disposal Sites (ODMDS) to ensure compliance with the MPRSA.

The MPRSA assigns basic responsibility to USEP A and USACE for ensuring that ocean dredged material disposal activities will not unreasonabl y degrade or endanger human health, welfare, or amenities, or the marine environment (MRP SA Sections 102 and 103). Section 102 of the MPRSA authorizes USEPA to designate sites or times at which dumping may occur and to establish criteria for reviewing and evaluating permit applications, including those for dredged material. It also authorized USEPA, in conj unction with USACE, to de velop site management plans for dredged material disposal sites. Sect ion 103 of the MPRSA authorizes USACE to issue permits subject to compliance with the USEPA environmental criteria (Ocean Dumping Criteria at 40 CFR Part 227) and USEPA concurrence with USACE’s finding of compliance. Section 103(b) authorizes USACE, with USEPA concurrence, to select alternative project sites of limited duration for disposal of dredged material in ocean waters when the use of a site designated by USEPA is not feasible.

Site designation has two primary purposes. Sites are selected that minimize adverse environmental effects and minimize the interference of dumping activities with other activities in the marine environment (USEPA 1986). Also, the site designation process is designed to ensure that temporary perturbations in water Chemistry are reduced to normal ambient seawater levels before reaching any beach, shoreline, marine sa nctuary, or geographica lly limited fishery or shellfishery [40 CFR § 228.5 (a, b)].

A site designation requires a formal evaluation of alternativ e sites in which the potential environmental impacts associated with disposal of dredged material at various locations are examined. Before the site designation process can begin, USACE must first demonstrate the need for the proposed ODMDS designation action [40 CFR 6.203(a) and 40 CFR 1502.13]. Once the need for an ocean disposal site is established, potential sites are screened for feasibility through the Zone of Siting Feasibility (ZSF) process. Once the ZSF process is completed, candidate sites are selected for further evaluation. Sites are evaluated using USEPA’s ocean disposal criteria at 40 CFR Part 228. The regulations ou tline five general criteria and 11 specific factors upon which to base site selection (40 CRF 228.5-228.6; Table 5-1 ). Of the sites that satisfy these criteria, the site which best complies with the criteria is selected as the preferred alternative for formal designation through rulemaking published in the Federal Register.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
13 

### Table 5-1. Five General and 11 Specific Site Selection Criteria

General Site Selection Criteria – 40 CFR 228.5

(a) The dumping of materials into the ocean will be per mitted only at sites or in areas selected to minimize the interference of disposal activities with other activities in the marine environment, particularly avoiding areas of existing fisheries or shellfisheries, and regions of heavy commercial or recreational navigation.

(b) Locations and boundaries of disposal sites will be so chosen that temporary perturbances in water quality or other environmental conditions during in itial mixing caused by disposal operations anywhere within the site can be expected to be reduced to normal ambient seawater levels or to undetectable contaminant concentrations or effect s before reaching any beach, shoreline, marine sanctuary, or known geographically limited fishery or shellfishery.

(c) If at any time during or after disposal site evaluati on studies, it is determined that existing disposal sites presently approved on an interim basis for ocean dumping do not meet the criteria for site selection set forth in Sections 228.5 through 228.6, the use of such sites will be terminated as soon as suitable alternate disposal sites can be designated.

(d) The sizes of the ocean disposal sites will be limited in order to localize for identification and control any immediate adverse impacts and permit the implementation of effective monitoring and surveillance programs to prevent adverse long-range impacts. The size, configuration, and location of any disposal site will be determined as a part of the disposal site evaluation or designation study.

(e) USEPA will, wherever feasible, designate ocean dump ing sites beyond the edge of the continental shelf and other such sites that have been historically used.
Specific Site Selection Criteria – 40 CFR 228.6(a)

(1) Geographical position, depth of water, botto m topography, and distance from the coast.

(2) Location in relation to breeding, spawning, nursery, feeding, or passage areas of living resources in adult or juvenile phases.
(3) Location in relation to beaches and other amenity areas.

(4) Types and quantities of wastes proposed to be disposed of, and proposed methods of release, including methods of packaging the waste, if any.
(5) Feasibility of surveillance and monitoring.

(6) Dispersal, horizontal transport, and vertical mixing c haracteristics of the area, including prevailing current direction and velocity, if any.

(7) Existence and effects of current and previous discharges and dumping in the area (including cumulative effects).

(8) Interference with shipping, fishing, recreation, mi neral extraction, desalination, fish and shellfish culture, areas of special scientific importanc e, and other legitimate uses of the ocean.

(9) Existing water chemistry and ecology of the site as determined by available data or by trend assessment or baseline surveys.
(10) Potentiality for the development or recruitmen t of nuisance species in the disposal site.

(11) Existence at, or in close proximity to, the site of any significant natural or cultur al features of historical importance.
Source: USEPA 1986Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
14 5.1 Purpose and Need/Site History The Jacksonville ODMDS is located on the continental shelf off Jacksonville, Florida, approximately 5 nmi southeast of the St. Johns River mouth ( Figure 5-1 ). It is 1 nmi by 1 nmi (1 nmi 2 ) in size with an average depth of 14 m (46 ft). Since 1952, the Jacksonville ODMDS and vicinity has been used for disposal of sand, silt, and clay dredge material from the Jacksonville Harbor Navigation Project, Naval Station Mayport entrance channel, and Naval Station Mayport turning basin. The Jacksonville ODMDS received interim site designation status in 1977 and final designation in 1983.

Due to potential capacity issues at this site, the USACE and USEPA have identified a need to either expand the Jacksonville ODMDS or designate a new ODMDS in the vicinity. The need for expanding current ocean disposal capacity is based on observed mounding, future capacity modeling, historical dredging volumes ( Table 5-2 ), and estimates of future proposed projects. The purpose of the proposed action is to ensure that adequate environmentally acceptable and economically and logistically feasible ocean dispos al site capacity is available for the next 50 years for suitable dredged material generated from new projects and maintenance dredging. The availability of suitable ocean disposal sites to support ongoing maintenance and capital improvement projects is essential for the continued use and economic growth of vital commercial and recreational areas in the region. As part of the site designation process, candidate sites are being evaluated based on environmental, operational, and economic criteria to identify a viable dredged material disposal site.

Disposal History and Future Needs Prior to 1970 and in the early 1970s, material was disposed in an area 0.5 nautical miles east of the Jacksonville ODMDS. In the late 1970s mate rial was disposed south of the site (USEPA and USACE 2007). Naval Station Mayport has used the Jacksonville ODMDS regularly since 1994 and has been taking all of its maintenance dredged material to the Jacksonville ODMDS since two upland dredged material placement sites on Naval Station Mayport reached capacity in 1993. Since 1995, Naval Station Mayport has used the Jacksonville ODMDS on a biannual basis for disposal of most of its 900,000 cubic yards (cy) of maintenance material (annual average 450,000 cy) (NAVFAC 2008). Between 1996 and 2006, a total of approximately 4.8 million cy of dredged material was placed at the Jacksonville ODMDS, of which 82% was generated from Naval Station Mayport maintenance dredging ( Table 5-2 ) (USEPA and USACE 2007). The need for maintenance material disposal from Naval Station Mayport is expected to continue at increasing levels with deepening of the turning basin and entrance channel, and the Jacksonville ODMDS is the closest and preferred disposal site.

In addition, the Jacksonville Harbor Federal Navigation Project has used the Jacksonville ODMDS for disposal of coarse material not suitable for beach placement from the entrance channel. In 2007, approximately 510,000 cy of ma terial was placed at the Jacksonville ODMDS for the federal navigation project ( Table 5-2 ). Currently, USACE removes approximately 300,000 cy from the outer portion of the federal navigation channel during maintenance dredging every three years (annual average 100,000 cy), which has been disposed of in the Jacksonville ODMDS since 2003 (NAVFAC 2008). It is expected that Jacksonville Harbor Federal Navigation Project will continue to utilize the ODMDS for non-beach-compatible material in the entrance channel. As upland disposal alternatives become limited, the volumes from the Jacksonville Harbor Federal Navigation Project may increase.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
15Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
16 

### Table 5-2. Projected 10-Year St atus of the Jacksonville ODMDS

Maintenance Volume 460,000 cy (Historical Annual Average) + 30,000 cy 1 (Projected Increase from Proposed Project) = 490,000 cy Annual Average

10-Year Maintenance Volume 10 x 490,000 cy (Average Annual Maintenance Projects) = 4.9 Million cy

Proposed NAVSTA Mayport Deepening 5.2 Million cy

10-Year Impact on LowVolume Estimate: 4.9 Million cy + 2.0 Million cy = 6.9 Million cy, Which is 96% of the Estimated Available 7.2 Million cy Capacity of Jacksonville ODMDS 2
cy = cubic yards
1 The projected increase of 27,500 cy annual maintenance dr edge increase resulting from the proposed deepening is conservatively rounded to 30,000 cy. Source: NAVFAC 2008
2 Based on the most recent MDFATE and LTFATE Mode ling submitted by USACE to USEPA on July 11, 2010.

Recent sediment transport modeling indicated a sedimentation increase of about 2% within the Naval Station Mayport turning basin, 7% within the Naval Station Mayport entrance channel, and 2% within the federal navigation channel (NAVFAC 2008). This equates to a ca. 5% increase of overall maintenance volume, or an annual increase of approximately 27,500 cy of maintenance material. In addition, it is estimated that the Jackso nville Harbor Federal Navigation Project will need to dispose of approximately 472,000 cy every three years for maintenance dredging due to shoaling at various sites along the project reach.

New work is also being proposed, including the Department of the Navy homeporting of additional surface ships at Naval Station Mayport, which would require deepening of the turning basin and entrance channel at Naval Station Ma yport and deepening of the Jacksonville Harbor Federal Navigation Project, Bar Cut 3 ( Figure 5-1 ). Total estimated volume for dredging is approximately 5.2 million cy with subsequent disp osals of maintenance dredged material of 0.8 million cy annually (NAVFAC 2008). The proposed Jacksonville Harbor deepening project will likely increase annual maintenance volumes as well ( Figure 5-2 ). It is estimated that up to 38 million cy of material will be generated for po tential new construction. There are also nonfederal projects that have dredged material dispos al needs. In general, ODMDS use is expected to increase as upland disposal sites become limited.

Currently, use of the Jacksonville ODMDS is temporarily restricted to 2 million cy per year as established in the 2007 Site Management and Monitoring Plan (SMMP) (USEPA and USACE 2007). This restriction is based on maximum histor ical uses of the ODMDS. Priority for ODMDS use within the capacity constraints will be given to maintenance projects from the Naval Station Mayport and the Jacksonville Harb or Federal Navigation Project. Approval for new work projects or maintenance material from non-federa l projects is dependent on a consideration of site capacity and long-term (i.e., 10 years) maintenance needs from the aforementioned maintenance projects.Sampling and Analysis Plan for JacksonvilleODMDS Fall 2010 Site Designation SurveySection 5.0, Element A5: Problem Definition/Background17Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 5.0, Element A5: Problem Definition/Background
18 It is recognized that the Jack sonville ODMDS requires expansion or a new site designated by USEPA to enable continued disposal of maintenance dredging from Naval Station Mayport, the federal navigation channel, and others that use Jacksonville ODMDS; therefore, 10 years of maintenance capacity are used to determine viability. Table 5-2 provides an estimate of the status of the Jacksonville ODMDS over the next 10-y ear period based on historic use of the site. The estimated remaining capacity is approximately 7.2 million cy which represents approximately 96% of its capacity.

5.2 Identification of Principal Data Users and Decision Makers
Table 5-3: Areas of Responsib ility for Principle Data Users
AgencyOrganization Location Area(s) of Responsibility

USACE— Jacksonville Jacksonville, Florida Responsible for maintenance and co-management of the Jacksonville ODMDS with USEPA, Region 4. Responsible for preparation of NEPA documents supporting new site designation or expansion of the Jacksonville ODMDS pursuant to Section 103 of the MPRSA.

USEPA, Region 4 Atlanta, Georgia Responsible for new site designation ODMDS or expansion of the Jacksonville ODMDS. Gives concurrence to environmental requirements of dredged sediment for approval for offshore disposal per the Green Book (USEPA 1991), SERIM (USEPA/USACE 1993), and the ITM (USEPA 1998), and co-management of the Jacksonville ODMDS with USACE—Jacksonville.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
19 6.0 ELEMENT A6 - TASK DESCRIPTION

6.1 Purpose/Background The designation of an ODMDS will be executed by the USEPA and USACE and will be based on:
Environmental studies of each si te, regions adjacent to the site, and on historical knowledge of the impact of dredged material disposal on areas similar to such sites in physical, chemical, and biological characteristics (Regul ations and Criteria, Part 228.4)

Before final designation can be conferred upon a site, full environmental documentation must be prepared and approved by the USEPA for each site (Pequegnat et al. 1990). All studies for the evaluation and potential selection of a new ODMDS will be conducted in accordance with five general criteria (40 CFR 22.5) and eleven specific criteria (40 CFR 228.6). The surveys described in this document are part of the required environmental documentation for the ODMDS site design ation process.

Based on the ZSF analysis and available data, several study areas were selected for more indepth evaluation to determine the suitability for dredged material disposal. In October 2009 and March 2010, sidescan sonar surveys, video transects, and dives were conducted from the USEPA OSV Bold to assess the existence of hard or liv e bottom resources within and adjacent to these study areas. Based on results from this survey, USEPA and USACE selected two candidate sites in which additional surveys described in this docu ment will be performed ( Figure 6-1 ).

The purpose of the site designation surveys is to further describe the environment of the candidate sites including sediment characteristics (physical and chemical), infauna, epifauna, and water chemistry characteristics. The obje ctive of these studies is to narrow down candidate sites to one preferred alternative based on the results. The goal of these surveys is to select the least environmentally damaging an d most economically feas ible and logistically practical site possible.

The information obtained during these surveys will be considered baseline data. Survey results will be used to determine whether future dredge d material placed at the new ODMDS has the potential to adversely impact benthic infaunal re sources, benthic epifaunal resources, sediment quality, or water chemistry. Information gleane d from this study and future studies will be used to guide management decisions relative to future disposal at the selected site.

ANAMAR has been contracted by USACE to provide field and project management support to USACE and USEPA. ANAMAR will work as a team with USACE and USEPA personnel to collect sediment, benthic, fish, and water chemistry samples for analysis of physical, chemical, and taxonomic parameters as outlined in this do cument. ANAMAR will manage subcontracting with laboratories and be responsible for transferring custody of samples to the appropriate laboratories. ANAMAR will also re ceive the laboratory reports, review all results and quality control, compile data, and submit a complete report that summar izes results and conclusions.Sampling and Analysis Plan for JacksonvilleODMDS Fall 2010 Site Designation SurveySection 6.0, Element A6: Dredging Project/Task Description20FIGURE 6-1a. Proposed Sampling Stations in and around Candidate Sites 1 and 2Sampling and Analysis Plan for JacksonvilleODMDS Fall 2010 Site Designation SurveySection 6.0, Element A6: Dredging Project/Task Description21FIGURE 6-1b. Proposed Sampling Stations in and around Candidate Site 3Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
22 6.2 Description of the Sampling and Analysis Two site designation surveys ar e planned for this project. The first survey was conducted during spring 2010 (March 28–April 2). This QAPP addresses the second survey, scheduled for fall 2010 (September 13–18). The purpose of conducting two surveys is to account for seasonal variability of the parameters of interest . The two surveys vary slightly and the fall survey will be described in more detail in the following sections. Objectives of the surveys focus on the characterization of selected areas of seafloor from a sedimentological, chemical, and biological perspective, but also include phys icochemical studies of the water column in the selected areas.

6.2.1. Measurements That Are Expected During the Course of the Fall Sampling Survey See Section 13.3 for proposed analytical method s and target detection lim its. Table 6.1 lists types of samples or measurements to be conducted by candidate site and station.

Table 6-1. Fall 2010 Field Sampling and An alysis Scheme for Jacksonville ODMDS Site Designation Sampling Event

Sample

Type Site Number ( Station Number ) Number of Samples Parameters / Analyses Collection Method 1

Water Chemistry 1 & 2
( S02 & S04 ) 1 Above and 1 Below Thermocline at S02 during Ebb Tide

1 Above and 1 Below Thermocline at S04 during Flood Tide
Total = 4 Samples Metals TSS Pesticides PAHs Niskin Bottles

Water Chemistry 3
( N05 & N09 ) 1 Above and 1 Below Thermocline at N05 during Flood Tide

1 Above and 1 Below Thermocline at N09 during Ebb Tide
Total = 4 Samples Metals TSS Pesticides PAHs Niskin Bottles

Water Chemistry TBD
( TBD ) 1 Field Split Sample Metals TSS Pesticides PAHs Niskin Bottles

Infauna 1 & 2 ( S01, S02, S03, S04, S05, S06, S07, S08, S12, & S13 ) 10 Stations X 3 Replicates Total = 30 Samples Taxonomic ID Various Indices Biomass Grab

Infauna 3
( N01, N02, 8 X 3 Replicates = 24 Total = 24 Samples Taxonomic ID Various Indices Biomass GrabSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
23 Sample

Type Site Number ( Station Number ) Number of Samples Parameters / Analyses Collection Method 1

N03, N04, N06, N07, N08, & N09 )

Epifauna 1 & 2 ( S02, S04, S05, S08, S12, & S13 ) 4 Stations X 2 Replicate Tows Inside Sites = 8 Tows
2 Stations X 2 Replicate Tows Outside Sites = 4 Tows Total = 12 Samples Total Biomass Taxonomic ID Species Richness

Other Indices Otter trawl

Epifauna 3
( N02, N04, N06, & N08 ) 2 Stations X 2 Replicate Tows Inside Site = 4 Samples
2 Stations X 2 Replicate Tows Outside Site = 4 Samples Total = 8 Samples Total Biomass Taxonomic ID Species Richness

Other Indices Otter trawl

Tissue Samples 1 & 2
( S02, S08, S12, S13 + S04 or S05 ) 1 Invertebrate and 1 Fish Tissue Sample from Stations:

S02, S08, S12, and S13 + Either Station S04 or S05 Total = 10 Samples Between Sites 1 & 2 Metals PCBs PAHs Organotins Pesticides Samples processed at laboratory

Tissue Sample 1 & 2
( Any Station ) 1 Field Split from Any Sample In or Adjacent to Sites 1 & 2 Metals PCBs PAHs Organotins Pesticides Samples processed at laboratory

Tissue Samples 3
( N02, N08 + N04 or N06 ) 1 Invertebrate and 1 Fish Tissue Sample from Stations:

N02 And N08 + Either Station N06 or N04 Total = 6 Samples Between Sites 1 & 2 Metals PCBs PAHs Organotins Pesticides Samples processed at laboratory

Tissue Sample 3
( Any Station ) 1 Field Split from Any Sample In or Adjacent to Site 3 Metals PCBs PAHs Organotins Pesticides Samples processed at laboratory

Clam Dredge 1 & 2 ( S02, S04, S05, S08, S12, & S13 ) 4 Stations X 1 Tow Inside Site = 4 Samples (2 Reps if Time)

2 Stations X 1 Tow Outside Site = 2 Samples (2 Reps If Time) Total = 6 Samples (12 if Time for Total Biomass Taxonomic ID Species Richness

Other Indices Clam DredgeSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
24 Sample

Type Site Number ( Station Number ) Number of Samples Parameters / Analyses Collection Method 1
Reps)

Clam Dredge 3 ( N02, N04, N06, & N08 ) 2 Stations X 1 Tow Inside Site = 2 Samples (2 Reps if Time)

2 Stations X 1 Tow Outside Site = 2 Samples (2 Reps if Time) Total = 4 Samples (8 if Time for Reps) Total Biomass Taxonomic ID Species Richness

Other Indices Clam dredge

In Situ Water

Parameters 1 & 2 ( S02, S04, & S08 ) 1 Station Inside Sites 1 & 2 (Within Boundaries) + 2 Stations Outside these Sites
Ebb and Flood Tides at Each Station
Total = 6 Profiles CTD Data 2 Temperature Salinity Dissolved O 2

Turbidity PAR CTDprofiler

In Situ Water

Parameters 3 ( N02, N05, & N09 ) 1 Station Inside Site 3 (Within Boundaries) + 2 Stations Outside Site 3

Ebb and Flood Tides at Each Station
Total = 6 Profiles CTD Data 2 Temperature Salinity Dissolved O 2

Turbidity PAR CTDprofiler

1 Details on collection methods are provided in Section 11.
2 ANAMAR/Paul Berman processing data.

6.2.2. Applicable Technical Quality Standards or Criteria Physicochemical Sediment Analysis —Sediment samples were taken during the spring 2010 survey and results were comp ared to published sediment screening values whenever appropriate. These levels are the threshold effect level (TEL) and the effects range-low (ERL). The TEL represents the concentration below which adverse effects are expected to occur only rarely. The ERL is the value at which toxicity may begin to be observed in sensitive species (Buchman 2008). Comparisons are used for reference only and are not intended for any regulatory decisions. The main objective of the sediment physical and chemical analyses was to help provide characteristics from which to co mpare the three candidate sites with one another as well as with adjacent areas.

Infauna Survey — In this document, the term ‘infauna’ refers to those organisms greater than 0.5 mm in length which live in sediment. Ma jor taxonomic groups which fit the infauna description include polychaete worms (e.g., lugworms, sandworms), bivalve molluscs (e.g., scallops, clams, oysters, mussels), amphipod cr ustaceans (also known as scuds), and sipunculid worms (also known as peanut worms).

Data derived from the benthic macroinvertebrat e (>0.5 mm size) infaunal community study is intended to help determine the biological charac teristics of each propos ed ODMDS site. Results of the study will be included in the preparatio n of an Environmental Impact Statement (EIS) where potential impacts will be assessed and discussed.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
25 Epifauna Survey —In this document, the term ‘epifauna’ refers to those organisms which live and/or feed primarily on or near the seafloor and which are of a size which allows capture and retention in a 7.3-meter otter trawl. Organism s fitting this description include echinoderms, shrimps, lobsters, crabs, and demersal (both benthic and benthopelagic) fishes.

Results of the trawl-based epifaunal survey are intended to assess the invertebrate community and fish assemblages associated with the seafloor. Similar to the infaunal survey, taxonomic data and tissue analysis of selected fish and invertebrate species will be used to characterize each site and compare the candidate sites. These data will additiona lly be included the resultant EIS.

Epifaunal tissue samples will be used to comp are the three candidate sites with one another and to compare the sites with adja cent areas. Results will be compared to published screening values whenever appropriate. These levels include the TEL and ERL. Comparisons are used for reference only and are not intended for any regulatory decisions.

Clam Dredge Sampling —The fall 2010 clam dredge survey is designed to sample larger infaunal community members than would normally be sampled during the previously-mentioned grab sampler-based infaunal survey. Large bivalves are of primary interest during dredge sampling. Wet Weight biomass will be collected from each sample. If time permits, data from non-bivalve organisms could also be collected. Burrowing echinoderms (especially sea cucumbers) should be set aside for the invertebrate zoologists from the Florida Museum of Natural History to sort through, as time allows.

Similar to the other faunal surveys, taxonomic data and tissue analysis of invertebrate species will be used to characterize ea ch site and compare the candid ate sites. These data will be included in the resultant EIS.

Physicochemical Water Studies —In situ parameters and water chemistry results will be used to characterize each site and compare the candidate sites. Additionally, these data will be used in future Automated Dredging and Disposal Alternatives Modeling System Simulations (ADDAMS) for dredged material disposal events to document compliance with USEPA marine water chemistry criteria.

6.2.3. Special Personnel or Equipment Requirements That May Indicate the Complexity of the Dredging Project

Use of the OSV Bold is required for performing surveys. The sampling equipment will be operated by Bold personnel. In addition to personnel provided by USEPA and USACE, two senior scientists and one fish biologist will be provided by ANAMAR. ANAMAR may also provide two invertebrate zoologists from the Florida Museum of Natural History having suitable specialized professional training which qualifies them to help sort epif aunal samples, and will be directly supervised by an ANAMAR scientist.

6.2.4. Assessment Techniques Needed for Site Designation Studies Sediment Physicochemical Analysis —Sediment collected during the spring 2010 surveySampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
26 was analyzed for physical and chemical parameters in the laboratory and then validated against criteria established in this document. The asse ssment techniques stated in sections 20 and 23 were used to provide sufficient assurance that th e quality objectives of the project will be met. Sediment samples will not be coll ected during the fall survey.

Sediment Infaunal Survey —Benthic infaunal invertebrates gr eater than 0.5 mm collected in benthic grab samples will be sorted and identified to lowest practical taxonomic group in the laboratory at Barry A. Vittor & Associates, Inc. and will be assessed using techniques stated in sections 20 and 23 of this document. The information contained within these sections is designed to provide adequate assurance that quality objectiv es will be met.

Epifaunal Survey —Epibenthic invertebrates and demersal fishes collected during the otter trawl surveys will be sorted and determined to lowest practical taxonomic group by ANAMAR scientists. The assessment techniques discussed in sections 20 and 23 of this document are designed to meet quality objectives of the project.

Clam Dredge Survey —Large bivalves such as clams and scallops collected by rocking chair dredge (or hydraulic dredge) will be weighed (wet weight), sorted, and determined to lowest practical taxonomic group by ANAMAR scientists with the assistance of Florida Museum of Natural History invertebrate zoology staff memb ers. The assessment techniques discussed in sections 20 and 23 of this document are design ed to meet quality objectives of the project.

In Situ Water Parameters —In situ water parameters will be recorded using a CTD profiler during field operations aboard the OSV Bold. Data recorded with the CTD will be postprocessed back in the office. Assessment tech niques will include those stated in sections 20 and 23 of this document and are designed to provide sufficient assurance that quality objectives of the project will be met.

Water Chemistry Analysis —Water samples from each cand idate site will be collected and sent to Columbia Analytical Se rvices, Inc. for laboratory analysis of selected chemical components using analytical methods specified in Section 13 of this document. Assessment techniques used in water analyses are stated in sections 20 and 23 of this document and are designed to provide su fficient assurance that quality obje ctives of the project will be met.

6.2.5. Schedule for the Work Performed The spring 2010 survey was co nducted during the period March 28–April 2, 2010. The fall 2010 survey is planned for the period September 13–1 8, 2010, with additional dates before and after the survey for dive reconnaissance of sidescan targets. The following table provides an estimated schedule for the completion of the work.

Table 6-2. Estimated Schedule of Work Performed

Responsibility Estimated Schedule for Sampling/Testing/Reporting Estimated Date ANAMAR Submit Draft SAP/QAPP to USEPA and USACE for Approval; Allow FDEP to Comment March 2010 (Completed) ANAMAR Address Comments and Finalize SAP/QAPP March 2010Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
27 (completed) USACE/USEPA/ANAMAR Conduct Spring 2010 Site Designation Study March 28–April 2, 2010 (Completed) ANAMAR Submit Sediment, Water, and Infaunal Samples to Laboratories for Analysis April 2010 (Completed) Laboratories (see section 4.3) Submit Spring 2010 Analytical Reports to ANAMAR July 2010 (Completed) ANAMAR Review Data from Spring Sampling Event: Analytical Reports, Begin Compiling Data Tables, Maps, and Figures July–August 2010 (Completed)

ANAMAR Submit Draft SAP/QAPP to USEPA and USACE for Approval Early September 2010

ANAMAR Address Comments and Fina lize SAP/QAPP Early September 2010 USACE/USEPA/ANAMAR Conduct Fall 2010 Site Designation Study September 13–18, 2010 ANAMAR Submit Infauna and Tissue Samples to Laboratories for Analysis September 2010

Laboratories (see section 4.3) Submit Fall 2010 Analytical Reports to ANAMAR December 2010

ANAMAR Review Analytical Reports, Compile Data Tables, Maps, and Figures, and Produce Draft Report for Submission to USACE/USEPA Early January 2011

USACE and USEPA Review Draft Report and Submit Comments to ANAMAR January 2011

ANAMAR Respond to Comments by USAC E and USEPA January–February 2011 ANAMAR Submit Final Report Containing Results of Both Surveys to USACE/USEPA February 2011

6.2.6. Required Deliverables/Reports
The following documents must be submitted:

1. Draft Sampling and Analysis Plan/Quality Assurance Project Plan (SAP/QAPP) submitted for review and comment.

2. Final SAP/QAPP following update from comments for final appr oval prior to sampling.

3. Field paperwork includes field sheets, Daily Quality Control Report, calibration sheets, etc. These sheets are described in Table 12-1 and are provided in Appendix B of this document. They are also provided as an electronic attachment on a compact disk.

4. Chemical Quality Assurance Report (CQAR). The CQAR is used to evaluate all of the representative data from the project field sampling and laboratory analyses. For each group of data, a data review checklist is completed that assesses daily field QC reports and specific QC chemical data quality indicators, and it enables the reviewer to identify potential data problem areas that may requir e additional data validation. The Quality Assurance Report identifies non-conformances, QC deficiencies, or other problems that would impact the data quality objectives as specified in the work plan and the QAPP. The CQAR summarizes the overall usability of the data for the intended purposes. This report will be an appendix to the Final Report.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 6.0, Element A6: Dre dging Project/Task Description
28 5. Draft Report submitted for review and comment —this report must in clude all supporting documentation, including field paperwork, laboratory reports, the chemical quality assurance report, and all correspondences among ANAMAR, USACE, and USEPA which address any modifications to the project.

6. Once approved by USACE and USEPA, the Final Report will be submitted which will address all comments.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 7.0, Element A7: Quality Object ives and Criteria for Measurement Data
29 7.0 ELEMENT A7 - QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT
DATA Table 7-1. Data Quality Objectives for Water and Tissue Analyses

Parameter QC Measurement Frequency Acceptance Criteria Storage/Holding Times All Organic Analyses

PAHs, PCBs, and Pesticides (Including Tissue Analysis) MB 1 per 20 Samples or 1 per Batch up to 20 Samples No Analyte Should be Detected > RL 14 dDays until Extraction, 40 Days thereafter MS/MSD 1 Set per 20 Samples or 1 Set per Batch up to 20 Samples 70–130% for Spike Limits 30% RSD for Precision

Duplicate 1 per 20 Samples or 1 per Batch up to 20 Samples 30% RSD for Precision (Evaluated for Analytes >300% RL) SRM** 1 per 20 Samples or 1 per Batch up to 20 Samples Within Limits Specified by Provider (Evaluated for

Analytes >300% RL) ICV Immediately Following Calibration Curve 80–120% Recovery

CCV Minimum—One per 10 Samples and at the End of Each Batch Whenever Batch is Greater than 10 or for GC/MS at the Beginning of Every 12 Hours RRF or RF ≤ 25% for GC/MS Methods and

≤ 15% for All Other Methods

Surrogates Every Sample 30–150% Recovery
Internal Standard Every Sample, where Applicable 50–200% Recovery

IC Verify after Each Initial Calibration <20% RSD for Each Analyte or RF ≤ 30% for GC/MS
MDL Verify MDL Study Once per Year for Each Analyte of Interest Updated Annually

Metals (Including Tissue Analysis) MB 1 per 20 Samples or 1 per Batch up to 20 Samples No Analyte Should be Detected > RL 180 Days

MS/MSD 1 Set per 20 Samples or 1 Set per Batch, up to 20 Samples 70–130% for Spike LimitsSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 7.0, Element A7: Quality Object ives and Criteria for Measurement Data
30 Parameter QC Measurement Frequency Acceptance Criteria Storage/Holding Times Duplicate 1 per 20 Samples or 1 per Batch up to 20 Samples 30% RSD (Evaluated for Analytes >300% RL)

SRM 1 per 20 Samples or 1 per Batch up to 20 Samples 70–130% Recovery (Evaluated for Analytes >3x RL) LCS/LFB 1 per 20 Samples or 1 per Batch up to 20 Samples 70–130% Recovery

ICV Immediately Following Calibration Curve 90–110% Recovery

CCV Minimum—One per 10 Samples and at the End of Each Batch Whenever Batch is Greater than 10 90–110% Recovery

LDR Verify LDR Once per Quarter for ICP Snalysis and One Time for Mercury Analysis Refer to Frequency

Initial Calibration for AA, Hg Performed Daily Correlation Coefficient ≥ 0.995

MDL Verify MDL Study Once per Year for Each Analyte of Interest Updated Annually

ICB Immediately after Initial Calibration No Analyte Should be Detected > RL

* If SRMs are not available, use laboratory control samples.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 7.0, Element A7: Quality Object ives and Criteria for Measurement Data
31 Table 7-2. Data Quality Objectives for Enumeration and Taxonomy of Invertebrates and Fishes

Parameter QC Measurement Frequency Acceptance Criteria Storage/Holding Times

Verification of

Taxonomic Identification and Enumeration Infauna (Invertebrates) > 10% of Samples Sorted by EachPerson will be Resorted and Reidentified by a Qualified Taxonomist > 95% Accuracy of Sorting and Identification Undetermined (Not Critical)

Epifauna (Invertebrates) 10% of Specimens/Station Re-identified by Qualified Biologist Zero (0) Misidentifications Undetermined (Not Critical)

Epifauna (Fishes) 10% of Specimens/Station Re-identified by Qualified Biologist Zero (0) Misidentifications Undetermined (Not Critical)

Clam Dredged Invertebrates 10% ofBivalves/Station Reidentified by Qualified Biologist Zero (0) Misidentifications Undetermined (Not Critical)Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 8.0, Element A8: Special Tr aining Requirements/Certification
32 8.0 ELEMENT A8 - SPECIAL TRAINING REQUIREMENTS/CERTIFICATION All sampling and field work will conform to the USACE Safety Manual EM 385-1-1 (USACE 2003).

All members of the sampling crew shall be trained in the correct procedures to ensure the samples collected are representative of the area and are not contaminated during handling. This training shall occur prior to any sampling. USEPA will address all sampling procedures, while ANAMAR will address procedures for sample handling directly related to homogenizing and compositing the sediment, and placing in sample containers. Taxonomic identification and enumeration of benthic epifauna will be performed by a staff bi ologist specifically qualified through a combination of formal training and pr ofessional experience to execute such tasks.

All ANAMAR field personnel are trained in cardio pulmonary resuscitation (CPR) and first aid. Certification must be updated annually for CPR and every three years for first aid. This training is required for anyone participating in field work. Training records are maintained at ANAMAR headquarters and each employee is provided with a card to carry indicating their training.

All laboratory personnel are required by NELAC to have a signed, dated initial demonstration of capability for any analysis they perform. Each in itial demonstration of ca pability is required to be updated annually. A document signed by the lab director, QA officer, and analyst is stored in the analyst’s records to veri fy both initial and continuing demonstration of capability.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 9.0, Element A9: Documentation and Records
33 9.0 ELEMENT A9 - DOCUMENTATION AND RECORDS

9.1 Reporting of Results
Table 9-1. Laboratories Submitting Data for this Project

Laboratory Analyses to be Performed Data Package Description

ANAMAR In situ Field Parameters,

Epifaunal Invertebrates and Fishes, Clam Dredged Fauna ANAMAR will Tabulate In situ Data and Submit as Part of the Report Epifauna: Invertebrate and Fish Identification, Weighed, Enumeration, Indices, etc.; Results Summarized and Discussed in Report. Clam Dredge Fauna: Identify Bivalves Captured, Weighed, Enumeration, Indices, etc. Results Summarized and Discussed in Report Columbia Analytical Services Metals, PAHs, PCBs, Pesticides, TOC (Sediment,

Water, Tissue Samples) Data Summary, Initial Calibration and Verification, Quality Control Including Blanks, LCSs, Duplicates, Spikes, Control Limits, Samp le Case Narrative, and Raw Data Package from Instruments

Barry Vittor & Associates, Inc. Infaunal Survey Taxonomic Identification and Enumeration Data Summary, Quality Control, and Narrative if Applicable

ANAMAR will provide summary tables of laboratory data including sample identification, analyte, results, limits for comparison, and qualifiers. The draft and final testing reports will undergo internal technical review and quality assurance review by persons with appropriate technical qualifications to ensure that the report meets th e project requirements specified in the technical work plan and the QA goals. Due to their large size, all original laboratory data packages will be submitted only as electronic files.

The report(s) will consist of standard size, 81⁄2-i nch by 11-inch, pages with oversize figures or tables folded, if necessary, to this size. The repo rt margins shall be suitab le for use in a durable 3-ring binder. A decimal numbering system will be used, with each section having a unique decimal designation. Reports that require extensive editing, have extensive errors, or are not in the required formats will be reject ed and re-submittal will be required . All submittals shall be sent to USACE—Jacksonville District and USEPA, Region 4. Any maps, drawings, figures, sketches, databases, spreadsheets, or text files prepared for this report shall be provided in both hard copy and digital form.

The digital copies of reports and other text documents shall be provided in Microsoft Word 2000 (or higher version). Sp readsheet files and data files shall be provided in Microsoft Excel 2000 (or higher version) format. All text, spreadsheet, and database files shall be delivered in compact disk read-only memory (CD-ROM) with ISO-9660 format. Level IV laboratory data shall be provided as Adobe Acrobat portab le document format (PDF) files.

Geographic data, including coordinates of all sa mpling stations, shall be provided in feet and projected into the Florida State Plane coordinate system.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 9.0, Element A9: Documentation and Records
34 One hardcopy and one electronic copy (provided on compact disk) of the final report shall be submitted to USACE—Jacksonville District and USEP A, Region 4. Other interested agencies or persons may request a copy of the final report. This report will also be included as an appendix to the EIS that will be completed for the Ja cksonville ODMDS site designation project.

9.2 Report Format The following paragraphs represent the format for el ectronic files being delivered as part of any contract. These paragraphs do not specify content or what the electronic files should contain. The content or data represented should be specified in the scope of work.
1. Specifications for Digital Data . Any maps, drawings, figures, sketches, databases, spreadsheets, or text files prepared under the terms of this contract shall be provided in both hard copy and digital form, unless otherwise specified in the scope of work. The hard copy deliverables shall be defined in the body of the scope of work. 2. Text, Spreadsheet, and Database Files : The USACE—Jacksonville District standard computing software is Microsoft Office. Re ports and other text documents shall be provided in Microsoft Word 2000 (or higher version) format and Adobe Acrobat Portable Document Format (PDF). Spreadsheet files sh all be provided in Mi crosoft Excel 2000 (or higher version) format. Databases shall be provided in Microsoft Access format, unless otherwise specified in the scope of work. Pr ior to database development, the contractor shall provide the Government with a Technical Approach Document and Entity Relationship Diagram for approval which describes the contractor's technical approach to designing and developing the database. All text, spreadsheet, and database files shall be delivered on compact disk read-only memory (CD-ROM) with ISO-9660 format. 3. Digital Mapping and Data Standards : The USACE—Jacksonville Dist rict utilizes MicroStation for computer-aided design and drafting (CAD D). Data provided must be readable by MicroStation SE or higher to provide design drawings, sketches, or figures. All digital files provided in MicroStation shall be provided in feet and projected into the Florida State Plane coordinate system. The maps shall use the GRS 1980 spheroid and the North American Datum of 1983 (NAD 83), and shall be provided on CD-ROMs. 4. Geographic Information System (GIS) Data Delivery Format a. Digital geographic maps and the related digital information sha ll be developed using double precision and delivered in uncompressed ArcInfo export file format (.e00) using ArcInfo Version 8.0 or higher. USACE—Jacksonville District will also accept ArcMap shapefiles (e.g., .shp, .shx, .dbf). These file formats are geographic information system softwareapplications produced by the Environmental Systems Research Institute and are in the GIS so ftware suite used by USACE—Jacksonville District and USEPA, Region 4. b. Digital geographic maps and the related digital information shall be usable on an IBM-compatible personal computer system using the Windows NT 4.0 or Windows 2000 operating systems. This data sha ll be provided on compact disk read-only memory (CD-ROM) wit h ISO-9660 format.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 9.0, Element A9: Documentation and Records
35 5. General Digital Standard for CADD and GIS Files
a. Geographic data shall be provided in feet and projected into the Florida State Plane coordinate system. The maps shall use the GRS 1980 spheroid and NAD 83. Vertical upland topographic surveys shall use the North American Vertical Datum of 1988 (NAVD 88). Hydrographic survey will reference the local dredging datum which will be provided in the projec t scope of services. No offsets shall be used. Each map layer or coverage shall have a projection file. Map or drawing scales will be determined by the Contracting Officer's Re presentative for the contract. Mapping accuracy for the agreed scales will co nform to the American Society for Photogrammetry and Remote Sensing (ASPRS) Accuracy Standards for Large-Scale Maps and Interim Accuracy Standards for Large-Scale Maps (ASPRS 1991). Copies of the ASPRS Accuracy Standards can be obtained by contacting: American Society for Photogrammetry and Remote Sensing 5410 Grosvenor Lane, Suite 210 Bethesda, Maryland 20814-2160 b. Geographic data must be provided in a form that does not require translation, preprocessing, or post proc essing before being used in the USACE’s system. However, the Contractor shall consult with the Government (specifically the Geographic Information Systems Coordinato r) concerning the use of alternative delivery formats such as MicroStation SE or higher to provide design drawings, sketches, or figures. All digital files prov ided in MicroStation shall be in the same projection and use the same coordinate system, datum, and units as stated above, and shall be provided on CD-ROMs. c. Geographic Data Structure: All geogra phic information shall be developed in a structure consistent with the Spatial Data Standards (SDS), Version 1.9or a higher version if available at the time of this pr oject. The Contractor shall consult with the Government concerning modifications or additions to the SDS. The Government may approve modifications to the Standard if it is determined that SDS does not adequately address subject data sets. Copies of the SDS may be obtained by contacting: Director, US Army Engineer Waterways Experiment Station Tri-Service CADD/GIS Technology Center Attn: CEWES-IM-DA/Smith 3909 Halls Ferry Road Vicksburg, Mississippi 39180-6199 d. Geographic Data Documentation: For each digital file delivered containing geographic information (regardless of format), the Contractor shall provide documentation consistent with the Content Standards for Digital Geospatial Metadata, June 1998 published by the Federal Geographic Data Committee. The documentation shall include but is not lim ited to the following: the name and description of the map layer or coverage, the source of the data and any related data quality information such as accuracy and time period of content, the type of data coverage (point, line, polygon, etc.), the field names of all attribute data and a description of each field name, the definition of all codes used in the data fields, the ranges of numeric fields and the meaning of these numeric ranges, the creation dateSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 9.0, Element A9: Documentation and Records
36 of the map layer and the name of the person who created it. A point of contact shall be provided to answer technical questions. Metadata generation software, referred to as Document.aml, is available from ES RI for use with ArcInfo to help in the production of the required metadata. Corpsmet95 metadata software is available from the US Army Geospatial Clearinghouse at http://corpsgeo1.usace.army.mil/. Copies of the FGDC metadata standard can be obtained by contacting:

FGDC Secretariat c/o U.S. Geological Survey 590 National Center Reston, Virginia 22092 (703) 648-5514

FGDC metadata standards can also be found on the Internet at: http://www.fgdc.gov

e. Geographic Data Review: The digital geographic maps, related data, and text documents shall be included for review in the draft and final contract submittals. The reviews may include a visual demonstration of the geographic data on the Windows NT computer system in the Envi ronmental Resources Se ction GIS Unit's. Actual installation of the digital data from the CD-ROM onto the computer will be conducted by GIS Unit personnel. However, the Contractor shall have a technical consultant available at each review to assist with any digital data discrepancies. The data will be analyzed for subject conten t and system compatibility. Review comments to data and text shall be incorporated by the Contractor prior to approval of the final submittal.
f. Ownership: All digital files, final hard-copy products, source data acquired for this project, and related materials, including that furnished by the government, shall become the property of USACE—Jacksonville and will not be issued, distributed, or published by the Contractor.

9.3 Data Reporting Package Archiving and Retrieval All reports, data, field sheets, correspondence, notes, field books, and any other documents associated with this project will be archived by the contractor for a minimum of five years from the date of the final report. Prior to disposal of any records, the contractor must contact the client (USACE—Jacksonville Distri ct) for authorization and direct ion in the disposal of said documents.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
37 

## GROUP B. PROJECT MANAGEMENT

10.0 ELEMENT B1 - SAMPLING PROCESS DESIGN

10.1 Scheduled Project Activities, Including Measurement Activities The spring 2010 survey was co nducted during the period March 28–April 2, 2010. The fall 2010 survey is scheduled for the period September 13 –18, 2010. Each survey will take an estimated four days plus one day of mobilization and one day of demobilization per survey.

Samples will be packed and transported to the resp ective laboratories i mmediately after return from the field.

10.2 Rationale for the Design

Sections 5.0 and 6.0 of this document descri be the rationale for conducting these site designation studies. Sample sites and para meters were determined in accordance with guidance from Section 102 and 103 of the MPRSA and 40 CFR 228, Revised Procedural Guide for Designation Surveys of Ocean Dredged Material Disposal Sites (Pequegnat, 1990), and SERIM (USEPA and USACE 2008).

10.3 Design Assumptions All sampling will be conducted un der the direct supervision of USACE—Jacksonville, USEPA Region 4, and ANAMAR. USEPA and USACE will address any concerns with field sampling and the possible relocation of sample sites, includ ing, but not limited to, the foreseen problems discussed below.

Possible foreseen problems and solutions include the following: Problem: Rock at a depth not allowing sample collection. Solution: Relocate sample location.

Problem: Heavy traffic in the sampling area limiting sample collection. Solution: Relocate sample loca tion(s), postpone sampling, or sample around traffic (safety dependent).

Problem: Adverse weather (e.g., hurricane, ligh tning, heavy rain) and/or rough seas. Solution: Postpone sampling until the situation clears.

Problem: Otter trawl becomes badly damaged during use. Solution: Repair trawl, or use an alternate trawl (OSV Bold has additional trawl gear).

Problem: Rocking chair dredge in unusable condition and irreparable. Solution: Conduct dredge sampling using hydraulic dredge (located on OSV Bold ).

Problem: Both dredges are in unus able condition and irreparable. Solution: Omit dredge sampling from survey unless another dredge can be obtained duringSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
38 survey (dredge sampling is optional and not discussed in gu idance documents).

All changes to the sampling protocol specifie d by this QAPP and the USEPA QAPP will be logged on the field sheets.

10.4 Procedures for Locating and Selecting Environmental Samples Fall 2010 sample stations for the collection of water, faunal tissue, infauna, and epifauna samples are listed below. Sample locations within the study areas were determined by USEPA and USACE—Jacksonville District. The sample identifications and coordinates are organized below in Tables 10-1 through 10-4. The sample IDs were derived from the location name (Jacksonville, JAX for shor t), plus the season (Fa is short for fall) and year (10 is derived from 2010), the station number (example: N01), and an abbreviated name of the sample type (INF is short for infaunal sample, EPI is short for epifaunal sample). Many sample IDs will have an additional suffix added to denote the replicate ID for each particular station.

Table 10-1. Infaunal Sample Identifications and Station Coordinates

Sample ID 1 Replicate 2 ID (3/Station) Sample Type & Description Latitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX-Fa10-N01-INF A B C

Candidate Site 3: 8 Infaunal Stations 3 Replicate Samples per Station (24 Total Samples for this Site) 30.53332466 81.3283426

JAX-Fa10-N02-INF A B C 30.50840910 81.3429191

JAX-Fa10-N03-INF A B C 30.49242385 81.3353077

JAX- Fa10-N04-INF A B C 30.49248986 81.3085738

JAX- Fa10-N06-INF A B C 30.47249195 81.3357043

JAX- Fa10-N07-INF A B C 30.47688254 81.3106165

JAX- Fa10-N08-INF A B C 30.46626911 81.2858312

JAX- Fa10-N09-INF A B C 30.44531565 81.2929732

JAX- Fa10-S01-INF A B C Candidate Sites 1 & 2: 10 Infaunal Stations 3 Replicate Samples per 30.36370728 81.30309265Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
39 Sample ID 1 Replicate 2 ID (3/Station) Sample Type & Description Latitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX- Fa10-S02-INF A B C Station (30 Total Samples for these Two Sites) 30.36311619 81.2873387

JAX- Fa10-S03-INF A B C 30.35000853 81.28421537

JAX- Fa10-S04-INF A B C 30.32153037 81.2966211

JAX- Fa10-S05-INF A B C 30.32230372 81.2841042

JAX- Fa10-S06-INF A B C 30.31529371 81.2933219

JAX- Fa10-S07-INF A B C 30.28450908 81.2723900

JAX- Fa10-S08-INF A B C 30.46626911 81.2858312

JAX- Fa10-S12-INF A B C 30.33336315 81.29842003

JAX- Fa10-S13-INF A B C 30.35000853 81.28421537

1 The ‘Fa’ portion of the sample ID stands for fall and allows for quick differentiation between the two

sampling events based on sample ID.
2 The replicate sample suffix will be added to each samp le ID. For example, the first sample collected at

Station 1 of the North Candidate Site during the spring sampling event will have the code ‘JAX-Sp10- N01-INF-A’ written or typed on the label. This code system is intended to allow easy differentiation between samples, including those taken in spring from those taken in fall.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
40 Table 10-2. Clam Dredge Sample Id entifications and Station Coordinates

Sample ID 1 Replicate 2 ID (1–2 per Station) Sample Type & Description Latitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX-Fa10-N02-CLA A B Candidate Site 3: 4 Clam Dredge Stations (2 Inside Site and 2 Outside Site) At Least 1 Tow per Station (2 Tows if Time Permits) (Between 4 And 8 Total

Samples for this Site) 30.50840910 81.3429191

JAX- Fa10-N04-CLA A B 30.49248986 81.3085738

JAX- Fa10-N06-CLA A B 30.47249195 81.3357043

JAX- Fa10-N08-CLA A B 30.46626911 81.2858312

JAX- Fa10-S02-CLA A B

Candidate Sites 1 & 2: 6 Clam Dredge Stations (4 Inside Sites and 2 Outside Sites) At Least 1 Tow per Station (2 Tows if Time Permits) (Between 6 and 12 Total Samples for These Two Sites) 30.36311619 81.2873387

JAX- Fa10-S04-CLA A B 30.32153037 81.2966211

JAX- Fa10-S05-CLA A B 30.32230372 81.2841042

JAX- Fa10-S08-CLA A B 30.46626911 81.2858312

JAX- Fa10-S12-CLA A B 30.33336315 81.29842003

JAX- Fa10-S13-CLA A B 30.35000853 81.28421537

1 The ‘Fa’ portion of the sample ID stands for fall and allows for quick differentiation between the two

sampling events based on sample ID.
2 The replicate sample suffix will be added to each samp le ID. For example, the first sample collected at

Station 1 of the North Candidate Site during the spring sampling event will have the code ‘JAX-Fa10- N02-CLA-A’ written or typed on the label. This code system is intended to allow easy differentiation between samples, including those taken in spring from those taken in fall.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
41 Table 10-3. Epifaunal (Trawl) Sample Identifications and Station Coordinates

Sample ID 1 Replicate 2
ID (2/Station) Sample Type & Description Latitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX-Fa10-N02-EPI A B

Candidate Site 3: 4 Epifaunal Stations (2 Inside Site and 2 Outside Site) 2 Replicate Tows per Station (8 Total Samples for this Site) 30.50840910 81.3429191

JAX-Fa10-N04-EPI A B 30.49248986 81.3085738

JAX-Fa10-N06-EPI A B 30.47249195 81.3357043

JAX-Fa10-N08-EPI A B 30.46626911 81.2858312

JAX-Fa10-S02-EPI A B

Candidate Sites 1 & 2: 6 Epifaunal Stations (4 Inside and 2 Outside the Two Sites) 2 Replicate Tows per Station (12 Total Samples for these Two Sites) 30.36311619 81.2873387

JAX-Fa10-S04-EPI A B 30.32153037 81.2966211

JAX-Fa10-S05-EPI A B 30.32230372 81.2841042

JAX-Fa10-S08-EPI A B 30.46626911 81.2858312

JAX-Fa10-S12-EPI A B 30.33336315 81.29842003

JAX-Fa10-S13-EPI A B 30.35000853 81.28421537

1 The ‘Fa’ portion of the sample ID stands for fall and allows for quick differentiation between the two

sampling events based on sample ID.
2 The replicate sample suffix will be added to each samp le ID. For example, the first sample collected at

Station 1 of the North Candidate Site during the spring sampling event will have the code ‘JAX-Fa10- N02-EPI-A’ written or typed on the label. This code system is intended to allow easy differentiation between samples, including those taken in spring from those taken in fall.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
42 Table 10-4. Tissue Sample Identifications and Station Coordinates

Sample ID 1 Replicate 2
ID (2/Station) Sample Type & Description Latitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX-Fa10-N02-TIS A B

Candidate Site 3: 1 Invertebrate and 1 Fish Sample

from Stations N02 and N08, + Invertebrate and Fish Samples from Either Station N04 or N06

(6 Total Samples for this Site) 30.50840910 81.3429191

JAX-Fa10-N04-TIS A B 30.49248986 81.3085738

JAX-Fa10-N06-TIS A B 30.47249195 81.3357043

JAX-Fa10-N08-TIS A B 30.46626911 81.2858312

JAX-Fa10-N10-TIS (field split 3 ) A 1 Tissue Sample Field Split (from any Site 3 Sample) TBD in Field TBD in Field

JAX-Fa10-S02-TIS A B

Candidate Sites 1 & 2: 1 Invertebrate and 1 Fish Sample from Stations S02, S08, S12, and

S13 + Invertebrate and Fish Samples from Either Station S04

or S05 (10 Total Samples for this Site) 30.36311619 81.2873387

JAX-Fa10-S04-TIS A B 30.32153037 81.2966211

JAX-Fa10-S05-TIS A B 30.32230372 81.2841042

JAX-Fa10-S08-TIS A B 30.46626911 81.2858312

JAX-Fa10-S12-TIS A B 30.33336315 81.29842003

JAX-Fa10-S13-TIS A B 30.35000853 81.28421537

JAX-Fa10-S14-TIS (field split 3 ) A 1 Tissue Sample Field Split (from any Sample from Sites 1 And 2) TBD in Field TBD in Field

1 The ‘Fa’ portion of the sample ID stands for fall and allows for quick differentiation between the two

sampling events based on sample ID.
2 The replicate sample suffix will be added to each samp le ID. For example, the first sample collected at

Station 1 of the North Candidate Site during the spring sampling event will have the code ‘JAX-Fa10- N02-TIS-A’ written or typed on the label. This code system is intended to allow easy differentiation between samples.
3 A field split will be taken from one tissue sample and will be coded in a way that disallows the laboratory

from learning which sample it was taken from.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
43 Table 10-4. In Situ (CTD-profiler) and Water Chemistry Station Identifications and Station Coordinates

Station ID 1 Sample Type Longitude (Dec. Degrees) Longitude (Dec. Degrees)

JAX- Fa10-N02-CTD A B Candidate Site 3: In Situ Water Column (Ctd-profiler) has 1 Station within the Site And 2 Stations Outside the Site Boundaries (Ebb and Flood Tides per Station) 30.50840910 81.3429191

JAX- Fa10-N05-CTD A B 30.48289339 81.3224904

JAX- Fa10-N09-CTD A B 30.44531565 81.2929732

JAX- Fa10-S02-CTD A B Candidate Sites 1 & 2: In Situ Water Column (Ctd-profiler) has 1 Station Within the Site and 4 Stations Outside the Site Boundaries (Ebb and Flood Tides per Station) 30.36311619 81.2873387

JAX- Fa10-S04-CTD A B 30.32153037 81.2966211

JAX- Fa10-S08-CTD A B 30.27672924 81.2879808

JAX-Fa10-N05-WC AT BT Candidate Site 3: Water Chemistry taken at Station N05 during Flood Tide Water Chemistry taken at Station N09 during Ebb Tide 2 Samples per Station [1 Sample Above Thermocline (‘AT’) and 1 Sample Below Thermocline (‘BT’)] 30.48289339 81.3224904

JAX-Fa10-N09-WC AT BT 30.44531565 81.2929732

JAX-Fa10-[xxx]-WC (field split 2 ) AT 1 Water Chemistry Field Split (from any Sample) TBD in Field TBD in Field

JAX-Fa10-S02-WC AT BT Candidate Sites 1 & 2: Water Chemistry Taken at Station S02 during Ebb Tide Water Chemistry Taken at Station S04 During Flood Tide 2 Samples per Station [1 Sample Above Thermocline (‘AT’) and 1 Sample Below Thermocline (‘BT’)] 30.36311619 81.2873387

JAX-Fa10-S04-WC AT BT 30.32153037 81.2966211

1 The ‘Fa’ portion of the sample ID stands for fall and al lows for quick differentiation between the two sampling events based on sample ID.
2 A field split will be taken from one sample and will be co ded in a way that disallows the laboratory from learning which sample it duplicates.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
44 Sample Analysis Table 10-5 summarizes the analyte groups to be tested for this project: a complete list of analyses and methods is shown in Section 13.3.1.

Table 10-5. Sample Analyses

Parameters Water Chemistry Samples Tissue Samples Infaunal & Large Bivalve Samples Epifaunal SamplesChemistry/Analyses Metals Y Y

— —
Pesticides Y Y

— — Organotins — Y — — PCB Congeners N Y

— —
PAHs Y Y

— —Taxonomic Analyses Gross Wt. Biomass

— — Y Y
Taxonomic Identification* — — Y Y

Species Richness

— — Y Y
Species Diversity — — Y Y
Species Abundance — — Y Y
Y = analysis will be performed. N = Parameters previously sampled in June 2009 around Jacksonville ODMDS (USEPA 2010a) and found not to exceed detection levels. Also PCBs are non-polar, making them practically insoluble in water (Patnaik 1999). — = analysis will not be performed/not required or not applicable. * = Determination of organisms will be to the lowest practi cal taxonomic level, which in most cases will be to the species level. Clam dredge samples will focus solely on large bivalves. Non-biva lve organisms will only be identified/enumerated if time permits.

Field Parameters All field measurements (e.g., temperature, dissolved oxygen, salinity, turbidity, photosynthetically active radiation) will be made using a CTD-profiler aboard the OSV Bold

. These data will be provided to ANAMAR for proc essing and submission in the draft and final reports.

10.5 Classification of Measurements as Critical or Noncritical Not applicable.

10.6 Validation of Any Nonstandard Methods

No modifications to methods are expected in this project. Any modifications will be coordinated and approved by the USACE and USEPA prior to implementation.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
45 11.0 ELEMENT B2 – SAMPLING AND METHODS REQUIREMENTS

11.1 Describe the Sample Collection, Preparation, and Decontamination Procedures All sampling will be done according to published procedures. In the absence of published clam dredge sampling guidance, the pr ocedures for trawl sampling will be modified for use with the clam dredge. General field methodologies and procedures follow those outlined in the Green Book (USACE/USEPA 1991) and procedures documented in the Florida Department of Environmental Protection Standard Operating Procedures for Field Activities ( DEP-SOP-001/01). Qualit y assurance and quality control procedures are based on those described in USEPA (1995). Sediment collection and storage procedures are based on those described in USEPA (2001). Epifaunal and infaunal sampling materi als and methods are based on those described in Pequegnat et al. (1990).

Field Sampling Schedule Sampling was performed in a span of four days at sea during the spring 2010 sampling event, and it is expected to take a similar amount of time during the fall 2010 sampling event. This schedule is dependent on several factors including but not limited to weather conditions, equipment functionality, and accessibility. Co ntact with facilities in the immediate area and local security forces (e.g., USGC) will be coordi nated prior to mobilizing to the field by USEPA and USACE. Contact information for all parties in volved as well as local facilities and security forces will be distributed to all part ies and will be on-hand in the field.

Sample Position Accuracy GPS station coordinates and sample depths will be recorded at the time of sampling by equipment and personnel aboard the OSV Bold . All parametric measurements will be provided by USEPA to ANAMAR for inclusion in the draft and final reports. For sediment and infaunal sampling, actual sampling locations will be within a 50 m range of the sampling stations. Water chemistry samples will be taken within 200 m of the sampling station. Epifaunal and clam dredge transect starting points (i.e., when the trawl or dredge first reaches the seafloor) will be within 200 m of the sampling station, and the dire ction of travel will be away from the station.

Sediment Sampling Sediment sampling was conducted during the spring 2010 survey. No further sediment sampling will be done during the fall 2010 survey.

Infaunal Sampling USEPA and USACE, in coordination with ANAMAR personnel, will collect a total of 54 infaunal replicate samples (18 stations x 3 replicates per station) during the fall 2010 sampling event. Samples will be collected with a Young-modified Van Veen grab with an internal volume of approximately 0.04 m 2 . Once on-deck, the sample will be inspected for signs of leakage from the grab sampler. If the grab sampler is de termined to be less than full, the sample is discarded and resampled. If the grab sampler is determined to be full, then the sample will be deposited into a stainless steel bin and carefully transferred into a standa rd US #35 mesh size (0.5 mm mesh size) sieve bucket. Each sample will be gently wet-sieved through the sieve bucket until all particles sma ller than 0.5 mm have passed thro ugh the screen. The remaining sediment and invertebrates are then gently washed into a cloth bag containing the sampleSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
46 label, and the bag is secured at the opening with two cable ties. Sample bags are then immediately placed into a bucket containing a fixative. Either undiluted NOTOXhisto (if provided by USEPA), or a 10% bu ffered formalin solution (supp lied by ANAMAR) will be used as a fixative in infaunal sample buckets. Buckets will be labeled to identify the samples contained within each. There is no thermal requirement for these samples once submerged in fixative, and samples are planned to be stored at ambient temperature during the sampling event and subsequent transport to ANAMAR headquarters. The samples will be shipped to Barry A. Vittor & Associates, Inc. for sorting and analysis following completion of each sampling event.

Clam Dredge Sampling Large bivalves (e.g., clams, mussels, scallops) will be collected in a 56-cm wide by 86-cm long and 36-cm high rocking chair dredge with an estimated dredge depth of 7.6 cm, but with adjustable tynes which allow a variety of penetrat ion depths. The stretch mesh size of the bag will be determined during mobilization onboard the Bold . If the rocking chair dredge proves inoperable, a hydraulic dredge will be substitute d. Sampled stations will be the same as for epifaunal (trawl) stations. Each sample will consist of a 10-mi nute tow at a speed of between 1.5 knots and 2 knots. This should prov ide a sampling area of approximately 830 m 2 to 1,100

m 2 of seafloor coverage per tow. Towing times may be modified somewhat if catch rates are deemed too low for adequate data gathering, or if too much biomass is contained within the bag, allowing sample loss. At least one dredge sample will be taken per station, but an additional sample will be taken per station if time permits.

Spatial coordinates will be record ed at the beginning and end of each tow, along with the direction of travel, water depth, tide sequence, estimated substrate based on trawl wear/catch, and weather parameters. Upon completion of each tow, specimens will be carefully and thoroughly removed from the dredge trawl. Each dredge sample will then be weighed (wet weight) using a 19-liter plastic bu cket with drain holes drilled in it and either a 5-kg or 20-kg hanging Macro Line scale having an accuracy of within 0.3% of maximum weight. Bivalves will be sorted, enumerated, and field-identified. A sub-sample of each bivalve species will be measured if logistically feasible (valve length in selected clam and scallop species). Non-bivalve organisms are of lesser importance during the clam dredge survey, but may be recorded if time allows.

It is possible that some specimens may be idea l for tissue sampling. If deemed desirable, tissue samples will be extracted from specimen s using methods discussed in the epifaunal tissue sampling section that follows.

Upon completion of field sorting and identification of dredged specimens, representative bivalve specimens will be retained for later identity confirmation. Specimens will be relaxed (if appropriate) using magnesium chloride solution , or with the addition of clove oil and then placed in 50–70% ethanol for long-term storage. Preserved specimens and associated field data will later be deposited and archived at the Florida Museum of Natural History Invertebrate Zoology collection to serve as vouchers and to allow further study by in terested researchers.

Epifaunal Sampling During both the spring and fall 2010 sampling ev ents, invertebrates and fishes will be collected in a 7.3 m wide otter trawl, with a liner of 14 mm stretch mesh in the bag end. A total of twoSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
47 tows will be conducted per sample d station. Each sample will co nsist of a 10-minute tow at a speed of between 1.5 knots and 2 knots. This should provide a sampling area of approximately 3,390 m 2 to 4,500 m 2 of seafloor coverage pe r tow, provided the trawl doors are positioned 7.3 m apart with the lead line touching the seafloor throughout the tow. Towing times may be modified somewhat if catch rates are deemed too low for adequate data gathering, or if too much biomass is contained within the bag, hinder ing analysis. Efforts will be made to tow after dark as well as during the day at each trawl station in order to more fu lly represent the benthic community. Spatial coordinates will be recorded at the beginning and end of each tow, along with the direction of travel, water depth, tide sequence, estimated substrate based on trawl wear/catch, and weather parameters . Upon completion of each tow, specimens will be carefully and thoroughly removed from the trawl net and bag, taking care not to overlook any still contained in the net (to avoid cross-contaminat ion between samples). Each trawl sample will then be weighed (wet weight) using a 19-liter pl astic bucket with drain holes drilled in it and either a 5-kg or 20-kg hanging Macro Line scale having an accuracy of within 0.3% of maximum weight. The specimens will be taxonomically identi fied and enumerated. If time permits, the first 10 individual fish per species from ea ch tow will be measured. Fish measurements will consist of standard length for teleosts, stretch total length for sharks, total length for skates, and disk width for stingrays. Selected inverteb rate species will be measured if time permits, and these measurements may include valve leng th in selected clam and scallop species, carapace width for selected crab species, and cara pace length for selected shrimp species. All data will be recorded on project- specific epifaunal field sheets.

During the fall sampling survey, a total of 16 tissue samples (invertebrates and fishes) plus 2 field splits will be taken. Using decontaminated st ainless steel fillet knifes and forceps, tissue samples will be extracted from muscle tissue (e.g., f illet of fish, tail of shrimp, claw of crab, soft portions of clam or scallop). Samples are measur ed as wet weights of tissue. A 4-gram portion of tissue will be used for metal analysis, a 30-gra m portion for PCBs, chlorinated pesticides, and organotins; and a 10-gram portion will be taken to test for PAHs [totaling 44 grams (1.6 ounces) minimum needed for analysis]. If a sp ecimen does not allow for at least 44 grams of tissue, multiple specimens of th e same species and similar size classes may be used from the same trawl sample. At least one of the tissue samples (ideally two samp les) will need to weigh at least 270 grams (9.5 ounces) for use in laboratory quality control an d triplicate runs of various analytes.

Taxa will be selected for tissue sampling and analys is based on a series of criteria including criteria given in Pequegnat et al. (1990), which suggests chosing edible taxa over inedible ones, and the sampling of both fish and shellfish. Ad ditional considerations include choosing taxa not known to undertake extensive migrations into ot her habitats, choosing ta xa having longer life spans over shorter life spans, and picking taxa having enough biomass to extract enough tissue for analysis. Finally, taxa closely related to those sampled during the Fernandina ODMDS October 1985 study may be useful for purposes of comparison. Taxa suitable for tissue sampling include, but are no t limited to, the following:

3⁄4 Mantis shrimp ( Gibbesia neglecta )

3⁄4 Rock shrimp ( Sicyonia spp.)

3⁄4 Portunid crabs ( Portunus spp.)Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
48 3⁄4 Spotted hake ( Urophycis regia )

3⁄4 Weakfish ( Cynoscion regalis )

3⁄4 Spot ( Leiostomus xanthurus )

3⁄4 Kingfish (AKA Whiting) ( Menticirrhus spp.)

3⁄4 Atlantic croaker ( Micropogonias undulatus )

Stainless steel fillet knives and forceps will be used to extract muscle on specimens selected for tissue analysis. Equipment contacting tissue sa mples will be first cleaned and decontaminated as described in Florida Department of Environmental Protection Cleaning/Decontamination procedures (FC 1000). Tissue sample meth ods and materials will follow the guidance of Pequegnat et al. (1990), but will di ffer in terms of the mass need ed for analysis and in the use of Teflon ® material in partial replacement of aluminum foil. Efforts will be made to extract as much muscle tissue as possible from each sampled in dividual in an effort to allow for laboratory splitting of samples and/or archiving of extra tissues for later use. Upon extraction of tissues, each sample will be wrapped in a portion of pre-cleaned Teflon ® bag and weighed using a 100- gram Micro Line hanging scale having an accuracy of within 0.3% of maximum weight. The Teflon ® -wrapped sample will then be wrapped in clea n aluminum foil. The wrapped sample will then be double-bagged in labeled Ziploc ® bags and stored frozen within a freezer onboard the ship. The temperature within th e freezer will be monitored usin g a thermometer and recorded on a temperature log. Upon conclusion of th e offshore survey, the tissue samples will be kept frozen for later delivery to the CAS laboratory.

Upon completion of field sorting and identification of trawl specimens, representative specimens from each trawl will be labe led and placed in Ziploc ® bags with drain holes (one bag per trawl sample) and the bags immersed in a 19 liter bucket and a fixative added so that there is more fixative than biomass in each bucket. A solution of 10% buffered formalin (1 part formalin to 9parts seawater) will be used as the fixative. Each bucket will then be sealed using a securefitting, water-tight lid. A separate bucket will be used per trawl sample, and a label will be added to the solution and another label affixed to the outside of the bucket. Sample codes will be written on the lid of ea ch bucket using an oil penc il or permanent ink marker.

Invertebrate specimens will be rela xed (if appropriate) using a chemical supplied by the Florida Museum of Natural History, Invertebrate Zoolo gy Collection, and placed in individual labeled containers containing 50–70% ethanol for long-ter m storage. Invertebrate and fish preserved specimens and associated field data will later be deposited and archived at the Florida Museum of Natural History Ichthyology and Invertebrate Zoology collections to serve as vouchers and to allow further study by in terested researchers.

Physicochemical Studies of the Water Column Just as in the spring survey, the fall 2010 sampling event will include in situ water parameters such as temperature, salinity, dissolved oxyge n, turbidity, and photosynthetically active radiation. These parameters will be recorded th roughout the water colu mn in each candidate site. Additional water profiles will be conducted at stations located aro und the candidate sites, to be chosen while in the field based on prev ailing current direction and influence of the St. Johns River. Data will be recorded via a cond uctivity, temperature, and depth profiler (CTD-Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
49 profiler) supplied by USEPA. Data obtained using the CTD-profiler will be given to ANAMAR for post-processing using Sea-Bird SBE data proce ssing software. Technicians onboard the OSV Bold are responsible for maintenance and calibration of the CTD-profiler.

In addition to the CTD data, a total of eight water chemistry samples (plus 1 field split) will be collected during th e fall 2010 survey. At each station, samples will be collected from above the thermocline and below the thermocline near the substrate. The field split sample can be taken from any of the other samples. Four 10-L Niskin bottles, attached in a rosette within a carousel sampling unit, will be supplied by the OSV Bold , while various pre-cleane d collection containers will be supplied by CAS. ANAMAR will decontamin ate the Niskin bottles with trace-metal-grade nitric acid and pesticid e-grade methanol. Efforts will be made to contain all rinsate and to avoid leakage of these chemicals. All wate r samples will be stored at less than 4  ̊ C on wet ice inside coolers and/or in a refrigerated unit for pr eservation. Samples will be delivered to CAS laboratory following completion of the fall 2010 survey.

Field Conditions Site conditions such as prevailing weather, wind direction, air temperatur e, and tidal cycle will be documented at each sampling site. In situ measurements, depth, date and time, coordinates, current conditions, sample descriptions, number of containers, and team members will be recorded on project- specific field sheets.

Decontamination All equipment contacting sediment or water samples will be cleaned and decontaminated as described in Florida Department of Environmental Protection Cleaning/Decontamination procedures (FC 1000). Below is a summarized list of procedures. Work surfaces on the sampling vessel will cleaned before the sampling da y begins and before leaving each station. Decontamination of all sample -handling equipment will occur be fore mobilizatio n and between sampling stations in order to prev ent any contamination of samples.

Decontamination Procedures (Excep t Niskin Bottle Decontamination)

• Wash and scrub to remove gross contamination

• Wash and scrub with Liquinox ®

• Rinse with tap or site water (depending on availability)

• Rinse with deionized water

• 2X Rinse with pesticide grade isopropanol

• 2X Rinse with deionized water

• Air dry

• Storage wrapped in new, clean aluminum foil Any derived waste will be contained and disposed of in accordance with fede ral, state, and local laws.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
50 Niskin Bottles Decontamination Procedures

• Wash and scrub to remove gross contamination

• Wash and scrub with Liquinox ®

• Rinse with tap or site water (depending on availability)

• Rinse with deionized water

• Rinse with trace-metal-grade nitric acid (contain rinsate in sealed container)

• Rinse with pesticide grade methanol (con tain rinsate in sealed container)

• 2X Rinse with deionized water

• Air dry

Field Splits/Duplicates One water chemistry field split sample will be co llected during the fall 2010 sampling event. A total of two field split tissue samples will be co llected during the fall 2010 sampling event. The field split samples will each be given unique identification codes from that of the native sample to ensure the samples are blind to the laboratories conducting the analyses.

Sample Homogenization No homogenization will be necessa ry during the fall 2010 survey.

Sample Handling Prior to Shipment to Laboratories After collection, the water chem istry samples will be immediately placed in properly labeled containers, and samples requiring thermal preservation will be stored in coolers and/or within one or more cold storage rooms onboard the OSV Bold . The temperature of the cold storage room(s) will be checked twice daily, once in the mo rning and once in the late afternoon or early evening, to ensure compliance with USEPA storage requirements. If the temperature in cold storage remains slightly above 4  ̊ C (5–6  ̊ C) for two consecutive readings, or if one reading is above 8  ̊ C, wet ice will be placed on and around the samples.

Tissue samples will be stored at or below 0  ̊ C (frozen) inside a freezer onboard the OSV Bold

. Infaunal samples will be stored without thermal preservation, within an aqueous solution of NOTOXhisto fixative. Representative fish spec imens from the trawl sampling will be stored in a solution of 10% buffered formalin within 19-L pl astic buckets, while representative invertebrates from the trawls will be stored in either 50–70% ethanol, or frozen, based on specimen sizes.

During transport from the field to ANAMAR headquarters, all samples requiring thermal preservation will be maintained at less than 4  ̊ C, with the exception of the tissue samples, which will be maintained at approximately 0  ̊ C (frozen). ANAMAR will use wet ice and/or a refrigerated trailer for thermal preservation of sediment and water sample s. Temperatures will be checked twice daily and will follow the guid elines shown below in Sample Storage and Transport for acceptance criter ia and corrective action. Tissue samples will be stored within a freezer while at ANAMAR headquarters and prior to shipment.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
51 Representative epifaunal fish specimens will be stor ed in a solution of 10% buffered formalin in 19-Liter plastic buckets with tight-fitting lids during transport to ANAMAR headquarters. Invertebrates captured during epifaunal su rveys will be stored and transported in 50–70% ethanol inside 19-Liter plastic buckets with tight-fitting lids. Infaunal samples will be stored and transported in undiluted NOTOXhisto fixative insi de 19-liter buckets. Infaunal samples will then be shipped to Barry A. Vittor & Associates, Inc. Epifaunal samples will be stored at ANAMAR until final species determinations are made, at which time representative specimens and associated data will be deposited at the Ichthyology an d Invertebrate Zoology collections at the Florida Museum of Natural Histor y in Gainesville, Florida.

Sample Storage and Transport Chain-of-custody forms will be filled out an d will accompany samples at all times during transport from ANAMAR headquar ters to the respective labora tory. The forms will note the sample ID and date and time of collection. Ea ch sample will have a unique alphanumeric code assigned to it which corresponds to those wr itten on the corresponding field sheets. The samples will be identified as specified in Tables 10-1 through 10-4. Afte r samples are logged in at the laboratory and assigned a unique laboratory ID number , they will be stored, handled, processed, and analyzed as described in the Quality Assurance Manuals and/or Standard Operating Procedures (SOPs) of the testing laboratories. Transport of samples from the port at Jacksonville to ANAMAR headquarters in Ga inesville will be accomplished using vehicles.

11.2 Identify Support Facilities for Sampling Methods All sampling will be conducted according to published procedures.

All sampling will be conducted aboard the OSV Bold . A former navy ship (ex-USNS Bold

, originally the USNS Vigorous ), the Bold is now equipped for ocean and coastal monitoring and has been owned and operated by the USEPA sinc e March 2004. This 224-foot long vessel has a 43-foot beam and a 15-foot draft and a hull disp lacement of 2,300-tons. This vessel has a sustained speed of 11-knots, has an operating crew of 19, and can sleep 20 visiting scientists. The OSV Bold is equipped for all sampling discussed in this document, including the collection of sediment, water, infauna, and epifauna samp les. Onboard facilities include laboratories designed to allow processing and analyzing of samples, and refrigerated units for thermal protected sample storage (USEPA 2010b).

ANAMAR will use sampling equipment aboard the Bold for collecting samples of infauna, epifauna, and water chemistry along with recording in situ water column readings. ANAMAR will provide stainless steel pans, sample contai ners, taxonomic literature, cameras, scales, measuring devices, certain fixatives, labels, field sheets, and coolers for use with various samples.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
52 Table 11-1. Standard Operating Pr ocedures used During Sampling
Standard Operating Procedure Description Sections Referenced FD 1000 Documentation Procedures Se ctions FD 1100, FD 1200, FD 2000, FD 3000, FD 4000, FD 4100, FD 5000, FD 5100, FD 5200 FC 1000 Cleaning/Decontamination Procedur e The applicable information from this SOP is shown in Section 11.1 of this QAPP. FS 2000 General Aqueous Sampling Sections FS 2000, FS 2001, FS 2002, FS 2003 FS 4000 Sediment Sampling Sections 1–3, Table FS 4000-1

11.3 Describe Sampling/Measurement System Failure Response and Corrective Action Process Any event that does not conform to the QAPP, SOPs, or SAP is considered a nonconformance event. Any such event will be identified as qu ickly as possible and reported to the project manager (PM) as soon as practical. If a nonconformance event happens in the fieldwork portion of this project, it will be documented in the DQCR. The project director and/or project manager will confer with USEPA and USACE—Jacksonville to outline a procedure for accomplishing the task so the quality of the pr oject is not compromised. Every effort will be taken to contact the USACE representative prio r to any deviation from the above-mentioned procedures.

11.4 Describe Sampling Equipment, Sample Preservation, and Holding Times
Sediment No sediment sampling is planned for the fall 2010 survey.

Infauna Refer to Section 11.1. Table 11-5 lists containe r requirements for infaunal taxonomic analysis.

Clam Dredging Refer to Section 11.1. Table 11-7 lists container requirements for clam dredge sample identity verification.

Epifauna Refer to Section 11.1. Table 11-4 lists the mi nimum volume needed for analysis of tissue chemistry and Table 11-6 lists container requirements for epifaunal identification verification.

Physicochemical Studies of the Water Column Refer to Section 11.1. Table 11-2 lists the mi nimum volume needed for analysis of water chemistry, and Table 11-3 listes container requirements for water sample analysis.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
53 Table 11-2. Minimum Volume Requiremen ts per Sample for Chemical Analysis

A For purposes of matrix spike analysis in the laborato ry, one sample will consist of at least 12.5 liters of water about three times the normal amount needed for metal and organic analyses). This is in addition to the one field split.

B For purposes of quality control in the la boratory, one sample will consist of a > 270-gram portion of tissue (more than seven times the normal amount need ed for metal and organic analyses). This is in addition to the one field split sample.

C Only tissue will be tested for PCBs and organotins (water will not be tested for these analytes).

Table 11-3. Container Requirements for Water Samples for Chemical Analysis
MATRIX - WATER

Parameters Container Type and Size Preservation Holding Time

Metals Teflon ®

, Glass, or

Plastic 1.5 L (3.5 L for Lab

QC of One

Sample) Nitric Acid to pH < 2 6 Months for All Metals Except Mercury; 14 Days for Mercury

Organics (Pesticides, PAHs) Amber Glass 3 L (9 L for Lab QC for One

Sample) Cool to <4  ̊ C 7 days for Sample Extraction, 40 Days After

Extraction for Analysis

Table 11-4. Container Requirements for Tissue Samples for Chemical Analysis
MATRIX - TISSUE

Parameters Container Type and Size Preservation Holding Time

Metals Teflon ® , And Aluminum Foil,DoubleBagged in Ziploc ® Bags Small Ziploc ® (Size Not Critical) Frozen Up to 1 Year if Kept Frozen (< 0°C)

Organics (Pesticides, PAHs, Organotins, PCBs) Teflon ® , and Aluminum Foil,Doublebagged in Ziploc ® Bags Small Ziploc ® (Size Not Critical) Frozen Up to 1 Year if Kept Frozen (< 0°C) Analytical Group Volume Water per Sample Volume Tissue per Sample Metals 1.5 L A 4 g Wet Weight B Organics (Pesticides, PAHs, PCBs C , Organotins C ) 3 L A 40 g Wet Weight BSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 11.0, Element B2: Sampling and Methods Requirements
54 Table 11-5. Container Requirements for Infaunal Samples for Taxonomic Analysis

Parameters Container Type and Size Preservation Holding Time

Benthic Infauna (Invertebrates) Cloth Bag Contained within a 19- Liter Bucket Ca. 1 L Bag Undiluted NOTOXhisto

or 10% Buffered Formalin Indefinite

Table 11-6. Container Requirements for Ep ifaunal Samples for Identity Verification and Possible Vouchering

Parameters Container Type and Size Preservation Holding Time

Epifaunal Invertebrates Various Ziptie Bags, Small Containers Based on Specimen Size 50–70% Ethanol Solution or Frozen (if

Large) Indefinite (Suitable for Long-term Storage)

Epifaunal Small/Medium Fishes Ziploc ® bags Contained Within 19-liter Plastic bucket 4 L Ziploc ® 10% Buffered Formalin or

Undiluted NOTOXhisto Change to 70% Ethanol Within 1 Month

Larger Fishes too Big

for 19-liter Bucket Large Plastic Bags 190 Liter Bag Frozen or on Wet Ice Eventually Fix in 10% Buffered Formalin, and Change to 70% Ethanol Within 1 Month

Table 11-7. Container Requirements for Clam Dredge Samples for Identity Verification and Possible Vouchering

Parameters Container Type and Size Preservation Holding Time

Bivalves, Other Invertebrates Various Ziptie Bags, Small Containers Based on Specimen Size 50–70% Ethanol Solution or Frozen (if

Large) Indefinite (Suitable for Long-term Storage)

Non-bivalve Invertebrates

(Optional) Various Ziptie Bags, Small Containers Based on Specimen Size 50–70% Ethanol Solution or Frozen (if

Large) Indefinite (Suitable for Long-term Storage)

All water samples will be preserved in cold storage or on wet ice to <4  ̊ C. All epifaunal tissue samples will be kept frozen (< 0  ̊ C) in a freezer or (briefly) on wet ice. A refrigerated trailer will be used to transport samples from the field to ANAMAR headquarters prior to shipment to laboratories. Holding times and preservation methods will conform to USEPA guidelines in QA/QC Guidance for Sampling and Analysis of Sediments, Water, and Tissues for Dredged Material Evaluations (USEPA 1995), if applicable.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 12.0, Element B3: Sample Handling and Custody Requirements
55 12.0 ELEMENT B3 – SAMPLE HANDLING AND CUSTODY REQUIREMENTS

Sample Handling All sample containers will be labeled accurately and properly. The label information will be consistent with that provided on the chain-of-custody form. Sample labels will include the following information: 1. Project 2. Sample Identification number and station number
3. Matrix 4. Date and time of sample collection 5. Name or initials of collector(s) 6. Sample preservation method used, if applicable

Sediment samples will be homogenize d as described in Section 11.1. Section 11.1 also explains procedures pertaining to sample storage and transport. The forms listed in Table 12-1 are used in the field to document in formation regarding the sample collection and handling.

Table 12-1. Field Activity Forms used to Document Sample Collection and Support Activities

All forms will be provided in the appendix of the final draft of this document.

Chain-of-Custody Requirements Chain-of-custody records from the field will have each sample or composite ID listed. Additional information will include sample date and time, analyses requested, containers shipped, File Name Purpose

Water Chemistry Sampling Field Sheet Records the information about how each sample was collected, as well as in situ measurements and si te conditions. This form also records the numbers of containers used, container type, and amount of water sampled. Infaunal Sampling Log Records information su ch as field conditions, description of sample, and materials and methods used in procuring it. Epifaunal (Trawl) Sampling Log Describes material s and methods of capture, waypoints, field conditions, and specimen information. Clam Dredge Sampling Log Describes materials and methods of capture, waypoints, field conditions, and specimen information. ANAMAR Chain-of-Custody Chain-of-custody form for shipping samples from ANAMAR to the laboratories for analysis Daily Quality Control Report The Daily Quality Co ntrol Report which provides any deviations from this QAPP, Region 4 guidelines, or any anomalous behavior of the samples. It is to be filled out on a daily basis after sample collection or any other field activities. Temperature Log Daily temperature log fo r recording sample temperature. Any deviations outside the acceptable limit will necessitate immediate corrective action to bring the samples back within control.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 12.0, Element B3: Sample Handling and Custody Requirements
56 relinquished by, date, and time, and receiver, date , and time. A copy of the chain of custody will be made and submitted with the final report.

Sample Shipping and Tracking ANAMAR personnel will deliver samples from the field to ANAMAR’s office. Sample shipping from the field or from ANAMAR to the laboratori es for analysis will use a common courier (i.e., Federal Express or Air and Surface Logistics). The laboratories will be contacted on the day of shipping to inform them of the shipment and tr acking number. The labo ratory will be contacted to confirm receipt of sa mples. If there is any delay, the courier will be contac ted to determine the time the samples will be delivered and any additional corrective action that may be necessary in the event of unexpected delays (e.g., weather-related or other causes). Should the delay cause the samples to rise above the acceptable temperature for storage, USACE and USEPA will be contacted and corrective actions will then be determined.

All coolers will have custody seals attached prior to shipment. Once rece ived, each custody seal will be inspected to determine if it is intact and the information reco rded on the chain-ofcustody form. If the custody seal is not rece ived intact, USACE and USEPA will be contacted to determine if corrective action is required.

Intra- and Inter-Laboratory Tracking Upon receipt at the laboratori es, the samples will be logged into the laboratory computerized information management system and assigned a unique number for tracking through the analytical process in the lab. Any lab transfer of samples (i.e., tissues, water, infauna) will have complete chain-of-custody records. All chain-of -custody records will be included in the final report to USACE.

Storage and Disposal of Samples The laboratories will retain all remaining unused sample volume under appropriate temperature and light conditions at least until the data ge nerated from the samples goes through ANAMAR QA/QC and is approved as acceptable. Preferably, samples will be retained until the final report is submitted to the USACE. Th e storage/archive time will be de pendent on space available at the laboratory. Approval by the USACE Project Officer will be ob tained prior to disposal of any sediment, water, or tissue sample if disposal is needed before the final report is submitted. Samples will be disposed of properly accord ing to federal, state, and local laws.

Additional information regarding sample storag e is presented in Section 11.1 (Sample Storage and Transport).Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
57 13.0 ELEMENT B4 – ANALYTICAL METHODS REQUIREMENTS

13.1 Subsampling Not applicable.

13.2 Preparation of the Samples Not applicable.

13.3 Analytical Methods

13.3.1 Physical and Chemical Analysis No sediment samples for physical analysis will be taken during the fall 2010 survey. CAS uses a reductive precipitation procedure to reduce the interferences from salts for metals analysis. Organic compounds are not affected by salt interferences. Epifaunal tissue sample analyses will be reported by CAS on both a wet and dry weight basis. In the resultant report, the tissue sample analyses will be summarizes on only a wet weight basis.

All analyses shall be performed in a timely manner in order to allow for retesting prior to expiration of holding times. If alternative methods or detection limits are used, approval from USACE and USEPA is required.

Tables 13-1 and 13-2 summarize analyses, methods and target detection limits for chemical parameters. Parameters in red within the tables indicate deviations from SERIM guidance. These deviations have been approved by the USACE and USEPA. The rationale is based on available historical data from the region. Some parameters have been omitted and some have been added. Please see footnotes for details.

Table 13-1. Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit METALS/OTHERS Arsenic 6010b/200.8 1 μ g/L Beryllium 6010b/200.8 1 μ g/L Cadmium 6010b/200.8 1 μ g/L Chromium 6010b/200.8 1 μ g/L Copper 6010b/200.8 1 μ g/L Lead 6010b/200.8 1 μ g/L Mercury 7470 0.2 μ g/L Nickel 6010b/200.8 1 μ g/L Selenium 6010b/200.8 2 μ g/L Silver 6010b/200.8 1 μ g/L Zinc 6010b/200.8 1 μ g/L Total Suspended Solids 160.1 5 mg/L PESTICIDES Aldrin 8081 0.5 μ g/L Chlordane & derivativesSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
58 Table 13-1. Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit Technical Chlordane 8081 0.5 μ g/L

α

(cis)– Chlordane 8081 0.5 μ g/L

γ

(trans) –Chlordane 8081 0.5 μ g/L Oxychlordane 8081 0.5 μ g/L Cis -Nonachlor 8081 0.5 μ g/L Trans- Nonachlor 8081 0.5 μ g/L DDT & derivatives o,p’ (2,4’)-DDD 8081 0.05 μ g/L p,p’ (4,4’)-DDD 8081 0.05 μ g/L o,p’ (2,4’)-DDE 8081 0.05 μ g/L p,p’ (4,4’)-DDE 8081 0.05 μ g/L o,p’ (2,4’)-DDT 8081 0.05 μ g/L p,p’ (4,4’)-DDT 8081 0.05 μ g/L Dieldrin 8081 0.01 μ g/L Endosulfan & derivatives Endosulfan I 8081 0.01 μ g/L Endosulfan II 8081 0.01 μ g/L Endrin & derivatives Endrin 8081 0.01 μ g/L Endrin aldehyde 8081 0.01 μ g/L Endrin ketone 8081 0.01 μ g/L Heptachlor & derivatives Heptachlor 8081 0.01 μ g/L Heptachlor epoxide 8081 0.01 μ g/L Hexachlorocyclohexane (BHC)

α -BHC 8081 0.01 μ g/L β -BHC 8081 0.01 μ g/L δ -BHC 8081 0.01 μ g/L

γ -BHC (Lindane) 8081 0.01 μ g/L Methoxychlor 8081 0.01 μ g/L Mirex © 8081 0.01 μ g/L Toxaphene 8081 0.2 μ g/L POLYNUCLEAR AROMAT IC HYDROCARBONS Acenaphthene 8270SIM/8310 0.005 μ g/L Acenaphthylene 8270SIM/8310 0.005 μ g/L Anthracene 8270SIM/8310 0.005 μ g/L Benzo(a)anthracene 8270SIM/8310 0.005 μ g/L Benzo(b)fluoranthene 8270SIM/8310 0.005 μ g/L Benzo(k)fluoranthene 8270SIM/8310 0.005 μ g/L Benzo(a)pyrene 8270SIM/8310 0.005 μ g/L Benzo(g,h,i)perylene 8270SIM/8310 0.005 μ g/L Chrysene 8270SIM/8310 0.005 μ g/L Dibenzo(a,h)anthracene 8270SIM/8310 0.005 μ g/L Fluorene 8270SIM/8310 0.005 μ g/LSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
59 Table 13-1. Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit Fluoranthene 8270SIM/8310 0.005 μ g/L Indeno(1,2,3-cd)pyrene 8270SIM/8310 0.005 μ g/L 1-Methylnaphthalene 8270SIM/8310 0.005 μ g/L 2-Methylnaphthalene 8270SIM/8310 0.005 μ g/L Naphthalene 8270SIM/8310 0.005 μ g/L Phenanthrene 8270SIM/8310 0.005 μ g/L Pyrene 8270SIM/8310 0.005 μ g/L

Table 13-2. Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weight) METALS/OTHERS Antimony 1 6010b/200.8 0.5 mg/kg Arsenic 6010b/200.8 0.2 mg/kg Beryllium 1 6010b/200.8 0.5 mg/kg Cadmium 6010b/200.8 0.1 mg/kg Chromium 6010b/200.8 0.5 mg/kg Copper 6010b/200.8 1 mg/kg Lead 6010b/200.8 0.2 mg/kg Mercury 7471 0.02 mg/kg Nickel 6010b/200.8 1 mg/kg Selenium 1 6010b/200.8 0.20 mg/kg Silver 6010b/200.8 0.062 mg/kg Zinc 6010b/200.8 1 mg/kg POLYNUCLEAR AROMATIC HYDROCARBONS Acenaphthene 8310/8270 SIM 20 μ g/kg Acenaphthylene 8310/8270 SIM 20 μ g/kg Anthracene 8310/8270 SIM 20 μ g/kg Benzo(a)anthracene 8310/8270 SIM 20 μ g/kg Benzo(b)fluoranthene 8310/8270 SIM 20 μ g/kg Benzo(k)fluoranthene 8310/8270 SIM 20 μ g/kg Benzo(a)pyrene 8310/8270 SIM 20 μ g/kg Benzo(g,h,i)perylene 8310/8270 SIM 20 μ g/kg Chrysene 8310/8270 SIM 20 μ g/kg Dibenzo(a,h)anthracene 8310/8270 SIM 20 μ g/kg Fluorene 8310/8270 SIM 20 μ g/kg Fluoranthene 8310/8270 SIM 20 μ g/kg Indeno(1,2,3-cd)pyrene 8310/8270 SIM 20 μ g/kg 1-Methylnaphthalene 8310/8270 SIM 20 μ g/kg 2-Methylnaphthalene 8310/8270 SIM 20 μ g/kg Naphthalene 8310/8270 SIM 20 μ g/kg Phenanthrene 8310/8270 SIM 20 μ g/kg Pyrene 8310/8270 SIM 20 μ g/kgSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
60 Table 13-2. Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weight) PESTICIDES Aldrin 8081 1.7 μ g/kg Chlordane & derivatives Technical Chlordane 8081 4.1 μ g/kg

α

(cis)– Chlordane 8081 1.7 μ g/kg

γ

(trans) –Chlordane 8081 1.7 μ g/kg Oxychlordane 8081 1.7 μ g/kg Cis -Nonachlor 8081 1.7 μ g/kg Trans- Nonachlor 8081 1.7 μ g/kg DDT & derivatives o,p’ (2,4’)-DDD 1 8081 1.7 μ g/kg p,p’ (4,4’)-DDD 8081 1.7 μ g/kg o,p’ (2,4’)-DDE 1 8081 1.7 μ g/kg p,p’ (4,4’)-DDE 8081 1.7 μ g/kg o,p’ (2,4’)-DDT 1 8081 1.7 μ g/kg p,p’ (4,4’)-DDT 8081 1.7 μ g/kg Dieldrin 8081 1.7 μ g/kg Endosulfan & derivatives Endosulfan I 8081 1.7 μ g/kg Endosulfan II 8081 1.7 μ g/kg Endrin & derivatives Endrin 8081 1.7 μ g/kg Endrin aldehyde 8081 1.7 μ g/kg Endrin ketone 8081 1.7 μ g/kg Heptachlor and derivatives Heptachlor 8081 1.7 μ g/kg Heptachlor epoxide 8081 1.7 μ g/kg Hexachlorocyclohexane (BHC)

α -BHC 8081 1.7 μ g/kg β -BHC 8081 1.7 μ g/kg δ -BHC 8081 1.7 μ g/kg

γ -BHC (Lindane) 8081 1.7 μ g/kg Methoxychlor 8081 1.7 μ g/kg Mirex © 8081 1.7 μ g/kg Toxaphene 8081 167 μ g/kg Total Chlorinated Pesticides 8081 10 μ g/kg SEMI-VOLATILES
Pentachlorophenol 2 8151 Modified or 8270C SIM 100 μ g/kg

ORGANOTINS Monobutyltin SERIM (USEPA and USACE 2008) 3 10 μ g/kg

Dibutyltin SERIM (USEPA and USACE 2008) 3 10 μ g/kg

Tributyltin SERIM (USEPA and 10 μ g/kgSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
61 Table 13-2. Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weight) USACE 2008) 3
PCB CONGENERS PCB-8 8082 1 μ g/kg PCB-18 8082 1 μ g/kg PCB-28 8082 1 μ g/kg PCB-44 8082 1 μ g/kg PCB-49 8082 1 μ g/kg PCB-52 8082 1 μ g/kg PCB-66 8082 1 μ g/kg PCB-77 8082 1 μ g/kg PCB-87 8082 1 μ g/kg PCB-101 8082 1 μ g/kg PCB-105 8082 1 μ g/kg PCB-118 8082 1 μ g/kg PCB-126 8082 1 μ g/kg PCB-128 8082 1 μ g/kg PCB-138 8082 1 μ g/kg PCB-153 8082 1 μ g/kg PCB-156 8082 1 μ g/kg PCB-169 8082 1 μ g/kg PCB-170 8082 1 μ g/kg PCB-180 8082 1 μ g/kg PCB-183 8082 1 μ g/kg PCB-184 8082 1 μ g/kg PCB-187 8082 1 μ g/kg PCB-195 8082 1 μ g/kg PCB-206 8082 1 μ g/kg PCB-209 8082 1 μ g/kg

1 This parameter has been added to the analyte list (not listed in SERIM).
2 This parameter has been omitted from the analysis (listed in SERIM).

3 The SERIM lists the method as ‘Krone et al., 1989’ but lacks the citation in the References section. This method refers to the Grignard reaction, gas chro matograph and flame photometric detector methods.

13.3.2 Taxonomic Analysis of Benthic Infauna Biological characterization of benthic infauna is to be performed by Barry A. Vittor & Associates, Inc. The tasks include sorting, identification, enumeration, and wet weight biomass of macroinvertebrate organisms collected at each station (see Barry A. Vittor & Associates, Inc. 2004 for further details). The contractor shall sort and identify all infaunal invertebrates to the lowest practical identification level. The contractor will also provide analysis and interpretation of the results for comparison between candidate sites using approaches designed to identify any difference s in community structure between stations and sites.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
62 The contractor shall calculate the following num erical indices for each station and site:

• Infaunal abundance (total number of individuals per station)

• Infaunal density (total number of individuals per square meter)

• Taxonomic richness (total number of taxa represented per station)

• Taxonomic diversity (distribution of individuals over species)

The contractor also shall perform data interpretation consisting of habitat characterization and benthic community characterization including faunal composition, abundance, and community structure. The contractor preparing the final repo rt shall also include a list of macroinvertebrate taxa for the surveys.

13.3.3 Taxonomic Analysis of Benthic Epifauna Characterization of trawl-caught invertebrate s and fishes will be performed by an ANAMAR biologist. Invertebrates and fishes will be enumerated and determined to the lowest practical taxonomic level (usually to species level, unl ess badly damaged during collection). Time permitting, select invertebrates will be measured. Crustaceans will be measured in carapace length or width, as appropriate, while other in vertebrates will be measured based on the most widely used method for that taxa. The first 10 individuals of each fish species from each trawl will be measured to the nearest millimeter. Teleosts will be measured in standard length, while elasmobranch metrics will be taken using the most widely used and accepted methods (i.e., stretch total length for sharks, disk width for non-rajid batoids, total length for rajids). For species represented by more than 10 specimens per trawl sample, only the first 10 specimens encountered will be measured, while all additional specimens will be counted and the number recorded on a field sheet.

For tissue chemistry samples, stainless steel f illet knives and forceps will be used to extract muscle tissues from epifauna. Equipment cont acting tissue samples will be first cleaned and decontaminated as described in Florida Department of Environmental Protection Cleaning/Decontamination procedures (FC 1000) . Tissue sample methods and materials will follow the guidance of Pequegnat et al. (1990), but will differ in terms of the mass needed for analysis and in the use of Teflon ® material in partial replacement of aluminum foil. Efforts will be made to extract as much muscle tissue as possi ble from each sampled individual in an effort to allow for laboratory splitting of samples and/or archiving of ex tra tissues for later use. Upon extraction of tissues, each sample will be wrapped in a portion of pre-cleaned Teflon ® bag and weighed using a 100-gram Micro Line hanging scale having an accuracy of within 0.3% of maximum weight. The Teflon ® -wrapped sample will then be wrapped in clean aluminum foil. The wrapped sample will then be double-bagged in labeled Ziploc ® bags and stored frozen within a freezer onboard the ship. The temperat ure within the freezer will be monitored using a thermometer and recorded on a temperature log. Upon conclusion of the offshore survey, the tissue samples will be kept frozen for later deliver y to the CAS laboratory. Prior agreement by the USACE project officer must be obtained befo re project methods are allowed to diverge from that of the procedural guide. Tissue samples will be stored frozen and shall be shipped to laboratories soon after completion of the fall sampling event.

Representative fishes not used in tissue sampling shall be placed in a 19-liter plastic bucket containing 10% buffered formalin solution, or un diluted NOTOXhisto for fixation. Large fishesSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
63 too big for a bucket will be frozen in large bags or kept on ice in a cooler. A label containing pertinent information shall be placed inside the bucket or bag and another label will be affixed to the outside of the bu cket. Representative invertebrates collected in the trawl survey will be relaxed using magnesium chloride or clove oil su pplied by the Florida Museum of Natural History Ichthyology Collection and later placed in 50–70% ethanol for long-term storage. All field data will be recorded on field sheets. Representa tive invertebrate and fish specimens will be deposited at the Invertebrate Zoology and Ichthyology collections at the Florida Museum of Natural History in Gainesville, Florida, to be us ed as vouchers and to allow for possible future verification of identification.

ANAMAR will provide, in the resulting report, the following analyses and data based on the trawl samples:

• Estimation of area sampled per trawl, and all trawls combined

• GIS figure showing trawl sampling locations and directions of travel

• Wet weight biomass per tow sample

• Numbers of individuals per major group (e.g., gastropods, shrimps, crabs, fishes)

• Numbers of most abundance taxa per station, and per site

• Numbers of federally-managed taxa per station and per site

• Community composition

• Notes on non-native introduced species, if found

• Taxonomic diversity and richness of benthic epifauna

• Results and discussion of tissue analyses

13.3.4 Taxonomic Analysis of Clam Dredge Samples Characterization of clam-dredged organisms will be will be performed by an ANAMAR biologist with the assistance of staff of the Florida Museum of Natural History. Bivalves will be weighed (collective wet weight), enumerated, and determined to the lowest practical taxonomic level (usually to species level, if possible without using genetic analysis techniques). If logistically feasible, a sub-sample of each bivalve species (such as 10 individuals/species) could be measured (valve length) and these measurements recorded in the field log.

Non-bivalve organisms are not of primary interest during clam dredge sampling and the recording of these data is optional. It would be useful to keep any macrophyte specimens (e.g., rhizomes) collected for later identifica tion, as these may represent nonindigenous species (e.g., Caulerpa brachypus

, C

. prolifera

, C

. taxifolia ) and such data is of pertinence to the site designation survey and may also be usef ul in verifying certain sidescan signatures.

It is possible that invertebrates captured during the clam dredge sampling may be useful for tissue sampling. If this is determined to be the case, tissue samples will be collected as in epifaunal sampling (see Section 13.3.3 above).

Representative bivalves (and any optional non-bivalve organisms) will be relaxed (if appropriate) using magnesium chloride or clove oil supplied by the Florida Museum of Natural History Ichthyology Collection and later placed in 50–70% ethanol for long-term storage. All field data will be recorded on field logs. After Representative invertebrate and fish specimensSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
64 will be deposited at the Invertebrate Zoology and Ichthyology collections at the Florida Museum of Natural History in Gainesville, Florida, to be used as vouchers and to allow for possible future verification of identification.

ANAMAR will provide, in the resulting report, the following analyses and data based on the clam dredge samples:

• Estimation of area sampled per dredge tow, and all tows combined

• GIS figure showing dredge-sampling locations and directions of travel

• Wet weight biomass per tow sample

• Numbers of individuals of each bivalve taxa per station, and per site

• Numbers of federally-managed bivalves (if any) per station and per site

If non-bivalve data is collected, optional analyses may include:

• Federally-managed non-bivalve organisms

• Species not collected in ep ifauna or infaunal sampling

• Community composition

• Notes on nonindigenous introduced species, if found

• Taxonomic diversity and ri chness of invertebrates

14.0 ELEMENT B5 - QUALITY CONTROL REQUIREMENTS All chemical analyses of water and tissues will be performed by CAS, a NELAC-certified laboratory. All analyses will be performed ac cording to NELAC standards. The Quality Assurance Manual (QAM) and SOPs for CAS clea rly specify quantitative and qualitative objectives for each analysis such as MDLs, precision, accuracy, completeness, representativeness, and comparability. The QAM and SOPs will be strictly adhered to for all analyses completed under the project. Standard quality-control checks including sample splits and replicates, blanks, spiked blanks, matrix spikes and duplicates, surrogate and internal standards, and calibration standards will be inco rporated into all laboratory activities and described in the laboratory’s QAM and SOPs.

All infaunal samples will be analyzed by Barry A. Vittor & Associates, Inc. and will undergo a quality control program as part of their standard operating procedures. At least 10% of all sorted specimens from a given sample will be re -sorted by another qualif ied taxonomist. If more than 5% of the re-sorted organisms are found to be in error in terms of identification, a quality control failure is logged and an additional sample is re-sorted. If the second re-sorting is found to be in error of grea ter than 5%, all samples previously sorted by that person are then re-sorted by another taxonomist. In addition, vo ucher collections are prepared for each project, composed of representative specimens from each species encountered in that project. These voucher specimens are labeled and archived for long-term storage and possible verification of identifications with outside specia lists. Senior taxonomists (having at least 5 years experience with a particular taxonomic group) check completed data sheets for questionable appearances of some taxa. Quality control is further accomplished by the labo ratory manager, who scrutinizes each data sheet prior to submittal to the data clerk for coding. With each new project, the laboratory manager completes a wr itten laboratory qualit y control report, with summarizes all phases of involvement. This qu ality control report is made available to the client.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
65 The QAM and SOPs for all laboratories performing analyses for this project list the analytical equipment used for testing, along with relevant calibration and standard reference materials used, maintenance schedules, and recordkeeping methods. The accuracy and precision limits included in the QAM and SOPs of the analytical laboratory will m eet the criteria established for this evaluation. The laboratory managers/direc tors listed in Section 4. 3 will be responsible for assigning appropriately-trained analysts to perform the specific tests. As part of the NELAC certification, corrective procedures have been established if QA objectives are not met. All laboratory QAMs are provided electronically on the attached CD.

Field analyses will be performed by ANAMAR pers onnel. Epifaunal and dredge sample field sorting may be assisted by trained individuals associated with the Florida Museum of Natural History at the University of Florida under the cl ose supervision of ANAMAR scientists. ANAMAR maintains strict quality assurance protocols in the field, including instrument calibration, decontamination, field custody shee ts and corrective action, if re quired. All quality control will be completely documented and included in the final report to the client.

USACE-Specific Data Quality Objectives
All chemical data must conform to th e data quality objectives listed in Table 7-1 of this document. For all data quality objectives th at are not achieved, the laboratory will provide documentation for the failed criteria and any corrective action taken and/or reanalyze the samples. All analytical anomalies will be described in detail in the final report.

Data Quality Objectives for Chemical Analyses Achieving the desired reporting limits (RLs) and me thod detection limits (MDLs) is critical to providing a suitable evaluation of contaminants of concern and the suitab ility of this site for disposal of sediment. The laboratory must perform yearly MDL verification studies on the matrices tested under this project.

The final report will include detailed explanations when the actual reporting limits exceed those listed in this table and/or when an altern ative test method is used. Any deviation from the proposed methods will receive prior approval from USACE—Jacksonville and USEPA.

Table 14-1 shows a list of common quality control activities that will be performed on project samples.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 14.0, Element B5: Quality Control Requirements
66 Table 14-1. Quality Control Activities Pe rformed for Field Activities and Chemical Analysis Quality Control Check Information Provided In Situ Measurements Provides onsite cond itions at the time of sample collection Initial Calibration Ensures instrument is operating correctly Initial Calibration Verification Initial Calibration Blank Ensures instrument ca libration will yield correct responses at a known concentration. This increases confidence that sample readings within the calibration range also are read correctly. Method Blank Ensure that the preparation and analysis of the samples is not introducing potential cont aminants through either the reagents used or other sources found through the laboratory. Standard Reference Material (SRM) Provides a known concentration of contaminant that can be used to verify the preparation and analysis of samples. The SRM should be as closely matched to the sample matrix as possible. Laboratory Control Sample/Laboratory Fortified Blank Provides a clean matrix sample which may be used to determine the instrument’s calibration is within acceptance limits. Continuing Calibration Standard Continuing Calibration Blank Verifies that the initial calibration is within control, and that any drift is within acceptable limits. Surrogates Verifies the analysis the individual contaminants performed by the an alytical method. Duplicates/Triplicates Measures the prec ision of the analytical technique, and can be used to determine the relative homogeneity of the sample. Matrix Spike/Matrix Spike Duplicate (MS/MSD) Used to measure the analytical bias of the test, and as an indication of matrix interferences in the sample.

ANAMAR will use the data quality review form fr om Appendix O of the SERIM for evaluating laboratory quality control. Th ese forms correspond to the tables shown in section 7.0 of this document.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 15.0, Element B6: Instrument/Equipment Te sting, Inspection, and Maintenance Requirements
67 15.0 ELEMENT B6 – INSTRUMENT/EQUIPME NT TESTING, INSPECTION, AND
MAINTENANCE REQUIREMENTS

Field Instruments: The field instruments to be used for this project will be provided by the USEPA aboard the Bold . Most calibration, operation, and maintenance will be the responsibility of Bold personnel, USEPA. Calibration of hanging scales, for use in measuring biomass, will be the responsibility of ANAMAR.

Laboratory Instruments: The QAM and/or SOPs for each labo ratory listed in Section 4.3 list the analytical equipment used for testing, along with relevant calibration and standard reference materials used, maintenance schedules, and recordkeeping meth ods. The accuracy and precision limits included in th e QAM and SOPs of the analytical laboratory meet the criteria established for this evaluation. The laboratory managers/directors liste d in Section 4.3 will be responsible for assigning appropriately-trained analysts to perform the specific tests. As part of the NELAC certification, corrective procedures have also been established if QA objectives are not met.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 16.0, Element B7: Instrument Calibration and Frequency
68 16.0 ELEMENT B7 – INSTRUMENT CALIBRATION AND FREQUENCY

Field Instruments All calibration of field instruments will be performed by USEPA personnel aboard the Bold

. Laboratory Instruments All laboratory instruments used in the analysis of sediment and site water analyses will be calibrated according to the method, laboratory QAM, SOPs, or any other NELAC-approved method. All records of calibration will also be documented and provided in the laboratory reports according to the above procedures. Any failure of the laboratory to achieve acceptable calibration will necessitate corrective action by th e laboratory in accordance with their QAM and SOPs.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 17.0, Element B8: Inspection/Acceptan ce Requirements for Supplies and Consumables
69 17.0 ELEMENT B8 – INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPLIES
AND CONSUMABLES Pre-cleaned glass bottles and Nalgene ® or equivalent bottles will be used for the storage of water chemistry samples. Certificates of Anal ysis will be kept by the laboratory according to their QAM and/or SOPs.

Equipment and supplies to be provided by personnel onboard the OSV Bold is expected to include positioning data (navigation and dept h feed) in DAC and wet lab, Inner space 448 Depth Sounder (paper recorder not necessary), extra monitor in the wet lab, Young-modified Van Veen grab sampler, 7.3 meter wide otter trawl, rocking chair dredge, CTD-profiler (sensors: conductivity, temperature, depth, dissolved oxygen, turbidity, PAR), with rosette of four 10-L Niskin bottles, sorting trays, temperature cont rolled freezers, and refr igerator. Expendable Supplies from the OSV Bold are expected to include writeable compact disks, cable ties, ice, paper towels, and de-ionized water.

The following equipment and supplies are expected to be provided by USEPA, Region 4. Data logging laptop, navigation laptop, DGPS, DGPS antennae with mounting pole, removable hard drive for data storage, sieve buckets, and cloth infaunal sample bags.

The remaining supplies and equipment necessa ry for sampling will be supplied by ANAMAR.

All laboratory consumables will be inspected, ha ndled, stored, documented, and used according to NELAC requirements and in accordance with each laboratory’s QAM and/or SOPs.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 18.0, Element B9: Data Acquisitio n Requirements (Non-Direct Measurements)
70 18.0 ELEMENT B9 – DATA ACQUISITION REQUIREMENTS (NON-DIRECT MEASUREMENTS) Various forms of data will be collected during fiel d and laboratory activiti es for this project: photographs, maps, GIS data, research-derived data, etc. All data generated during this project will be retained by the contractor. Any data no t required to be submitted as described in Section 6 will be supplied to US ACE or USEPA upon request.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 20.0, Element C1: Assessments and Response Actions
71 19.0 ELEMENT B10 – DATA MANAGEMENT, INTERPRETATION, AND REDUCTION

19.1 Data Management Each laboratory has established NELAC-approved procedures for data management, collection, validation, reduction, and reporting. As such, the analytical results will be extensively reviewed in-house by the laboratories submitting the data.

Each laboratory will submit an Electronic Data De liverable (EDD) and a hard copy data packet to ANAMAR. All data tables will be generated from the EDD and will be cross-checked against the hard copy data packet. When a data packet is re ceived by the contractor, it will be reviewed by the contractor’s QA/QC Officer, with emphasis on NELAC standards. All laboratory reports received will include laboratory QC data genera ted in the analysis of the project samples including results of all method blanks, lab duplicates, matrix spikes, spike duplicates, reference material, surrogate spik es, standards, check standards, an d calibration verifications. The analytical results for of these QC samples will be reviewed and documented in a CQAR for each analytical data packet received. This report will be incorporated into the final data report. The CQAR consists of a checklist and a case narrative of the analytical runs. Any nonconformance, QC deficiency, or other problems that would impa ct data quality will be addressed in the CQAR. In particular, the contractor will compare data to the data quality objectives listed in Section 7.0, as well as confirm that targ et detection limits listed in Sectio n 13.3.1 were achieved. If any data quality objective is not achieved, the la boratory will re-analyze the sample(s) and/or provide documentation for the failed criteria. The CQAR will contain a written record of the validity of each data package and its subsequent use in the report.

Field parameters, sample descriptions, site conditions, and additional information pertaining to the sample and sampling process will be recorded on site-specific field sheets. A DQCR will be filled out for each day of sampling and sample processing. Each of these records is integral to the successful completion of this project. As such, they will be reviewed, reported, and retained as described elsewhere in this document.

The contractor will use the password-protected C lient Login section on the company’s website to upload documents for client access and to keep USACE—Ja cksonville up-to- date with all documents and project-related data. This provides access to current documents and allows USACE—Jacksonville to perform an in-progress data review.

19.2 Data Interpretation and Reduction Reduction of chemical and physical data in th e final report will be done as discussed in the Green Book and the SERIM. See Sections 13.3.2 through 13.3.4 for data analysis and interpretation for infaunal, epifaunal, and clam dredge sampling data.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 20.0, Element C1: Assessments and Response Actions
72 

## GROUP C. ASSESSMENT AND OVERSIGHT

20.0 ELEMENT C1 – ASSESSMENTS AND RESPONSE ACTIONS ANAMAR’s QA/QC Officer is responsible for all assessments and response actions throughout the life of this project and is performed in-part through the review and audit process.

Performance and systems audits are performed to evaluate the capability and performance of a measurement system. Audits are utilized to en sure that field and laboratory activities will provide data reflective of site conditions and within project QA/QC requirements. A performance audit is used to evaluate the accuracy of a measurement method or component of the method. A systems audit focuses on evalua ting the principal components of a sample collection or data collection meth od to determine proper selection and use of that method.

ANAMAR’s field sampling teams will be overseen and directed at all times by the Project Manager. Field teams are audited periodically , usually annually, by the Contractor Project Director, company President, or QA/QC Officer. These audits entail an observation and critique of sampling methods, collection, preservation, labeling, handling of sediment and/or water samples to ensure SOPs are being followed, that all equipment is calibrated and used properly, the Health and Safety Plan is being followed, an d all aspects of the project are on schedule. Documentation of these audits is retained by Contractor.

Each laboratory used in this project regularly undergoes audits in accordance with their NELAC certifications. Although no proj ect-specific laboratory audits are scheduled for this project, any deficiencies identified in a recent audit that may adversely affect the data quality for this project will be brought to the attention of the ANAMAR Project Manager.

Any event that does not conform to the SAP/QA P, SOPs, or QAMs is considered a nonconformance event. These will be identified as qu ickly as possible and reported to the Project Manager as soon as practical. If the nonconformance event happens in the fieldwork portion of this project, it will be documented in the DQCR. The Project Mana ger will confer with the USACE—Jacksonville Project Officer or QA/QC Offi cer and outline a proced ure for accomplishing the task so the quality of the project is not co mpromised. Every effort will be taken to contact the USACE and/or USEPA representative prior to any deviation from the procedures documented in this SAP/QAPP.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 21.0, Element C2: Reports to Management 73 21.0 ELEMENT C2 – REPORTS TO MANAGEMENT

Each laboratory shall include as part of its Quality Assurance Program periodic reports to management. These reports will include internal au dits with corrective actions, findings with corrective actions of any external audits, either by regulatory agencies or clients, results of proficiency testing samples, and any routine issues directly re lated to quality assurance or control within the laboratory that has been found since the most recent audit. These reports should be maintained as part of the permanent record of the laboratory for a minimum of five years. Any report which may have a direct impact on th e samples included in this project should be addressed with the ANAMAR Project Manager or QA/QC Officer in order to determine the appropriate corrective action. All correspondence which may have an impact on the overall quality of the results will be included in the final report to the client.

Once received from the laboratories, the results will be incorpor ated into text, tables, and charts to more readily allow for evaluation by USACE and USEPA. These documents will be available to ANAMAR’s management for technical review before delivery to USACE.

Infauna Report

Table 21-1. Minimum Report Requirements

1. Introduction including summary of project background and study objectives. 2. Field methods including sample collection and handling and laboratory infaunal sample analysis. 3. Presentation of sediment physical and chemical analyses, with a comparison between stations and sites. 4. Presentation of water chemistry analyses, with a comparison between stations and sites. 5. Presentation of in situ water parameters with a comparison between stations and sites. 3. Analytical and statistical methods includin g assemblage analyses and faunal similarities.

4. Discussion of habitat characteri stics (e.g., grain size, water depth). 5. Faunal composition, abundance, and community structure. 6. Numerical classification analysis. 7. Tabular summary of infauna, epifauna, and dredge samples by taxonomic group. 8. Comparisons of stations within and adjacent to candidate sites. 9. Summary and discussion of federally managed taxa captured. 10.Comparisons to previous surveys (if applicable). 11. Brief discussion of quality assurance/quality control steps.

12. Results discussionSampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 21.0, Element C2: Reports to Management
74 13. Summary and conclusions.

In addition, the contractor shall provide raw data in electronic (digital) format. Two raw data files shall be provided by the contractor in accordance with Table 21.1 and shall be provided in Microsoft Excel, Extensible Markup Languag e (XML), or comma delineated format. The contractor shall also provide statis tical worksheet and output files.

Table 21-2. Electronic Raw Data File Format File Description Content/format

Abundance Data For each station and taxon collected, provide the following: station, phylum, class, family, genus, species, Number collected, and abundance Biomass Data For each station and major taxonomic group, provide the following: station, major taxonomic group, abundance.

Abundance Statistical Worksheet The format should have all sample si tes as column headers and all species as row headers with the number of indivi duals of that species for that site in the cell (including stations wher e a given taxon was not observed).

Biomass Statistical Worksheet The format should have all sample si tes as column headers and all species as row headers with the biomass of a given species and a given site in the cell (including stations where a given taxon was not observed).Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 22.0, Element D1: Data Review, Va lidation, and Verification Requirements
75 

## GROUP D. DATA VALI DATION AND USABILITY

22.0 ELEMENT D1 – DATA REVIEW, VA LIDATION, AND VERIFICATION
REQUIREMENTS

Data Validation Data validation is a process used to accept or reject data and determine if the data are traceable, defensible, and can be used for a partic ular project. Each laboratory has established state-approved procedures for data collection, vali dation, reduction, and reporting. As such, the analytical results will be extensively reviewed in-hou se by the laboratories submitting the data.

Section 7.0 contains tables showing the acceptan ce criteria for the analytical groups to be performed for this project. All physical and chem ical results shall be compared to the reporting limits in Section 13.3.1. All QC results for phys ical and chemical analyses will be compared to the tables in Section 7.0.

ANAMAR’s QA/QC Officer will review all laboratory results in accordance with the procedures described above. Any data which does not meet the acceptance criteria in the data tables will be qualified in bold italics with a shaded background. After review, ANAMAR’s QA/QC Officer, Project Manager and project di rector will perform an overall project review and recommend whether to accept or reject the data. US EPA and USACE will have the final authority to determine the acceptability of the project data.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 23.0, Element D2: Valida tion and Verifications Methods
76 23.0 ELEMENT D2 – VALIDATION AND VERIFICATIONS METHODS Laboratory Data Validation When a physical or chemical data packet is received, it is review ed by ANAMAR’s QA/QC Officer, with emphasis on NELAC standards and conformance to the data quality objectives set forth in this QAPP. Chemistry data reports include labora tory QC data generated during analysis of the project samples including results of all method bl anks, lab duplicates/triplicates, matrix spikes, spike duplicates/triplicates, refe rence material, surrogate spikes, standards, check standards, and calibration verifications. All phys ical testing reports will include sample duplicates/triplicates. The analytical results for these QC samples are reviewed and documented in a CQAR for each analytical da ta packet received. This report is then incorporated into the final data report. The CQAR consists of a checklist and a case narrative of the analytical runs. Any nonconformance, QC deficiency, or other problem that would impact data quality will be addressed in the CQAR. ANAMAR will compare data to the data quality objectives listed in Section 7.0, as well as conf irm that target detectio n limits listed in Section 13.3 were reached. If any data quality objectiv e is not reached, the laboratory will re-analyze the sample(s) and/or provide do cumentation for the failed crit eria. The CQAR will provide a written record of the validity of each data package and its subsequent use in the report. After all data have been reviewed and validated, an assessment of the impact on the data quality and usability of the results will be made and included in the report to the client.Sampling and Analysis Plan for Jacksonville ODMDS Fall 2010 Site Designation Survey

Section 24.0, Element D3: Reconcilia tion with Data Quality Objectives
77 24.0 ELEMENT D3 – RECONCILIATION WITH DATA QUALITY OBJECTIVES All chemical and physical data will be reconc iled by ANAMAR’s QA/QC Officer with the data quality objectives listed in Section 7.0 and with th e target detection limits listed in Section 13.3 by comparison with the laboratory results. Analytic al data that fall outside of the acceptable QC limits will be addressed in a case narrative and an y corrective action taken will be described in detail. Quality control failures which are no t adequately addressed will be re-prepared or reanalyzed at the laboratory’s expense. All analytical anomalies will be described in detail in the final report. In the case of reruns, the initial and rerun result will be presented in the final report.

Many analytical methods describe procedures for analytical anomalies that occur during analysis. These method-specific procedures must be followed.25.0 REFERENCES

Barry A. Vittor & Associates, Inc. 2004. Draft Quality Assurance Project Plan for Benthic Macroinvertebrate and Sediment Sample Analysis and Data Interpretation for Ocean Dredged Material Disposal Sites . Report submitted 26 April 2004 to USACE, Region 4.
Buchman, M.F. 2008. NOAA Screening Quick Reference Tables . NOAA OR&R Report 08-1, Seattle, WA, Office of Response and Restoration Division, National Oceanic and Atmospheric Administration. 34 pages.

Naval Facilities Engineering Co mmand, Southeast. 2008. Draft EIS for the Proposed Homeporting of Additional Surface Ships at Naval Station Mayport, FL.

Patnaik, P. 1999. A Comprehensive Guide to the Hazardous Properties of Chemical Substances Second Edition . John Wiley & Sons, Inc., New York, NY.

Pequegnat, W.E., B.J. Gallawa y, and T.D. Wright. 1990. Revised Procedural Guide for Designation Surveys of Ocean Dredged Material Disposal Sites . Department of the Army, Waterways Experiment Station, Corp s of Engineers, Vicksburg, MS.

U.S. Environmental Protection Agency. 1995. QA/QC Guidance for Sampling and Analysis of Sediments, Water, and Tissues fo r Dredged Material Evaluations – Chemical Evaluations . EPA-823-B-95-001. Accessed online at: www.epa.gov/waterscience/library/s ediment/evaluationguide.pdf.

U.S. Envir onmental Protection Agency. 2001. Methods for Collection, Storage, and Manipulation of Sediments for Chemical and Toxicological Analyses . EPA-823-B-01-002. October, 2001. Accessed online at: http://www.epa.gov/waterscience/cs /library/collectionmanual.pdf.

U.S. Environmental Protection Agency. 2002. Guidance for Quality As surance Project Plans(G5) [G-5 publication] (PDF 401KB) - December 2002, EPA/240/R-02/009. (Note: This document replaces EPA/600/R-98/018 issued in February 1998.) Accessed online at: www.epa.gov/quality/qs-docs/g5-final.pdf.

U.S. Environmental Protection Agency. 2006. National Recommended Wa ter Quality Criteria: 2006 . November. EPA-822-R-02-047. Accessed online at: www.epa.gov/waterscience/c riteria/nrwgc-2006.pdf.

U.S. Environmental Protection Agency. 2010a. Jacksonville, Florida Ocean Dredged Material Disposal Site (ODMDS) Survey, June 18–19, 2009. Final Report

. April 15, 2010 . USEPA Region 4, Science and Ecosystem Support Division, Athens, GA.

U.S. Environmental Protection Agency. 2010b. Ocean Survey Vessel (OSV) Bold

. Accessed online at: www.epa.gov/bold/brochure.html.

U.S. Environmental Protection Agency and USACE. 1991. Evaluation of Dredged MaterialProposed for Ocean Disposal-Testing Manual ( Green Book )

. EPA-503/8-91-001. February 1991. Accessed online at: www.epa.gov/owow/ocean s/gbook/gbook.pdf.

U.S. Environmental Protection Agency and U.S. Army Corps of Engineers. 2008. Southeast Regional Implementation Manual (SERIM) for Requirements and Procedures for Evaluation of the Ocean Disposal of Dredged Material in Southeaste rn U.S. Atlantic and Gulf Coast Waters . U.S. Environmental Protection Agency Region 4 and U.S. Army Corps of Engineers, South Atlantic Division, Atlanta, GA. Accessed online at: http://www.epa.gov/region4/water/oceans/ documents/SERIM_Fina l_August 2008 .pdf.Appendix 80 

# Appendix

CTD-profiler & Water Chemistry Sampling Log Infaunal Sampling Log Clam Dredge Sampling Log Epifaunal (Trawl) Sampling Log Chain-of-Custody Form Daily Quality Control Report Temperature LogAppendix 81Appendix 82Appendix 83Appendix 84Appendix 85Appendix 86Appendix87Appendix88