# Test Material: IN-J0290 (transformation product of nicosulfuron)

MRID 49169701

Title: Aikens, P.J. 2001. Adsorption/Desorption of [ 14 

# C]IN-J0290 in Five Soils.

EPA PC Code: 129008 (for parent compound)

OCSPP Guideline: 835.1230

For CDM Smith

Primary Reviewer: Kindra Bozicevich Signature:

Date: 9/2/14

Secondary Reviewer: Joan Harlin Signature:

Date: 9/2/14

QC/QA Manager: Joan Gaidos Signature:

Date: 9/2/14

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

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# Adsorption and desorption of IN-J0290, transf ormation product of nicosulfuron, in five soils.

Report: MRID 49169701. Aikens, P.J. 2001. Adsorption/Desorption of [ 14 

# C]INJ0290 in Five Soils. Unpublished study performed by Huntingdon Life Sciences Ltd., Cambridgeshire, England; sponsored and submitted by E.I. du Pont de Nemours and Company, Wilmi ngton, Delaware. DuPont Project ID DuPont-5264. Huntingdon Project ID DPT 564. Experimental initiation February 23, 2001 and completion May 25, 2001 (p. 11). Study completion date November 2, 2001. Document No.: MRID 49169701 Guideline: OCSPP 835.1230 Statements: The study was conducted in accordance with USEPA Title 40 CFR Part 160, UK GLP regulations, EC Commissi on Directive 1999/11/EC, and OECD Principles of GLP (p. 3). Signed a nd dated Data Confidentiality, GLP, Quality Assurance, and Certification of Authenticity statements were provided (pp. 2-5). Classification: This study is Acceptable . The study was conducted using a transformation product rather than the parent co mpound. The study was conducted using only three types of soil (loamy sand, silt loam, and sandy loam). Mass balances were determined for soils treat ed at the highest test concentration, rather across all test concentrations. PC Code: 129008 (for parent compound) Reviewer: Gabriel Rothman Signature: Environmental Scientist Date: August 20, 2015

Executive Summary

In a batch equilibrium study, four foreign soils (pH range 5.2-7.8) and one U.S. soil (pH 5.5) were used to measure sorption coefficients of [pyrimidine-2- 14 

# C]IN-J0290 in darkness at ca . 20- 22  C. Adsorption phase was conducted using five te st concentrations, and the desorption phase was conducted using only the highest test con centration. Determined Freundlich Adsorption Coefficient (K F 

# ) values, ranged from 0.86 to 45.21 L/kg (mean K F 

# = 10.39 L/kg); respective K FOC 

# values ranged from 59 to 1458 L/kg (mean K FOC 

# = 458 L/kg). 1 

# Freundlich Desorption Coefficients (K F-des 

# ) (range 0.002 to 680.6 L/kg, mean K F 

# = 137.9 L/kg) were higher than those obtained for the initial sorpti on measurement, indicating a de gree of irreversible sorption. Percent adsorption was not reporte d. Percent desorption, as percent of the adsorbed, ranged from 21 to 47% of the applied for all test so ils. Freundlich exponents corresponding to K F 

# values

1 

## Sorption is a generic term that applies to absorption, adsorption, and desorption processes. Adsorption refers to sorption onto a two-dimensional surface; ab sorption refers to sorptio n into a three dimensional matrix. Both types of sorption occur in soils and sediments. Desorption refers to a sorbate becoming desorbed from a sorbent. Desorption distribution coefficients are measured by removing solution from a sorption experiment and adding fresh solution, so that all material measured in solution will be the desorbed material. The guideline uses the term adsorption in place of sorption and refers to the initial measurem ent of sorption.

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# ranged from 0.71 to 0.86 across all test soils fo llowing the adsorption phase and from -2.34 to 2.86 following the second desorption step.

Table 3 summarizes the adsorption coe fficients measured in the study. Table 4 summarizes the desorption coefficients measured in the study.

Overall recovery was determined only for soils that were treated at the high est test concentration. Recoveries ranged from 97.0 to 100.6% of the applied for all test soils.

Coefficients of variation (CV) were 1.9 to 1.3 across all [ 14 

# C]-treated soils for adsorption K F 

# and K Foc 

# , respectively, and 2.2 and 2.2 across all [ 14 

# C]-treated soils for desorption K F 

# and K Foc 

# , respectively.

Results Synopsis:IN-J0290 (PC 129008 for Nicosulfuron) MRID 491697014 of 31Table 1. Summary of Adsorption/Desorption ResultsASoil/ Sediment,% OCpHRegressed Kd(L/kg-soil)Range ofKd(L/kgsoil)RegressedKOC(L/kgOC)KF((L/kg-soil)-1/n)1/n foradsorptionKFOC(L/kg-OC)-1/n)CeqRange(mg/L)Value ±SEr2p-valueValue ± SEValue ±SEr2p-valueValue ±SEValue ±SEAdsorptionSpeyer 2.2Loamy sand(2.1% OC, pH6.4)1.4 ±0.00.996 4.77E-12 1.32-2.71 66 ± 1 1.2 ± 1.0 0.998 0.001 0.86 ± 0.0 59 ± 500.0035-0.5131Pavia Loamysand (0.5% OC,pH 5.2)2.8 ±0.10.996 4.3E-12 2.70-7.00 560 ± 12 2.3 ± 1.0 0.998 1.6E-07 0.81 ± 0.0 452 ± 2100.0019-0.3719Drummer Siltloam (3.1% OC,pH 5.5)69.8 ±3.80.974 1.8E-0863.78-391.252251 ± 122 45.2 ± 1.1 0.993 4.8E-10 0.71 ± 0.0 1458 ± 360.0004-0.2018NambsheimSandy loam(0.7% OC, pH7.8)0.9 ±0.00.986 1.1E-09 0.89-2.61 135 ± 5 0.86 ± 1.0 0.998 0.007 0.79 ± 0.0 122 ± 1490.0036-0.5991Vercelli Siltloam (1.2% OC,pH 5.8)3.0 ±0.00.997 5.4E-13 2.91-7.28 247 ± 4 2.4 ± 1.0 0.999 2.6E-10 0.82 ± 0.0 197 ± 850.0018-0.3528DesorptionSpeyer 2.2Loamy sand(2.1% OC, pH6.4)5.3 ±0.20.998 0.030 5.07-5.57 253 ± 12 0.002 ± 1.0 1.0 N/A -2.3 ± 0.0 0.1 ± 480.08837-0.09086Pavia Loamysand (0.5% OC,pH 5.2)7.7 ±0.00.999 0.003 7.70-7.77 1547 ± 7 680.6 ± 1.0 1.0 N/A 2.9 ± 0.0136117 ±2000.0903-0.0907Drummer Siltloam (3.1% OC,pH 5.5)160.2 ±5.80.999 0.023154.63-166.245168 ± 187 0.8 ± 1.0 1.0 N/A -0.9 ± 0.0 26 ± 320.0631-0.0655IN-J0290 (PC 129008 for Nicosulfuron) MRID 491697015 of 31Soil/ Sediment,% OCpHRegressed Kd(L/kg-soil)Range ofKd(L/kgsoil)RegressedKOC(L/kgOC)KF((L/kg-soil)-1/n)1/n foradsorptionKFOC(L/kg-OC)-1/n)CeqRange(mg/L)Value ±SEr2p-valueValue ± SEValue ±SEr2p-valueValue ±SEValue ±SENambsheimSandy loam(0.7% OC, pH7.8)3.0 ±0.00.999 0.005 3.01-3.06 434 ± 4 7.5 ± 1.0 1.0 N/A 1.4 ± 0.0 1065 ± 1430.08571-0.08968Vercelli Siltloam (1.2% OC,pH 5.8)7.5 ±0.10.999 0.008 7.42-7.60 625 ± 8 0.7 ± 1.0 1.0 N/A -0.1 ± 0.0 55 ± 830.1008-0.1031Abbreviations: SE = standard error of regression.AData were obtained from Tables 5-9, pp. 33-37 and Tables 12-16, pp. 40-44 of the study report and DER Attachment. Data and results are in the attached Excelworkbook. See Attachment 3 for equations used for calculations.N/A = Not applicable.

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# I. Material and Methods

A. Materials

1. Test Material: [Pyrimidine-2- 14 

# C]IN-J0290 (p. 15) Batch/Lot number: 0318 Specific radioactivity: 141.8 μCi/mg (314800 dpm/μg) Radiochemical purity: >95.0% (by HPLC; p. 25; Appendix 1, pp. 55-56) Purity: Not reported

Solubility in water: Not reported

2. Reference Compounds: IN-J0290 (p. 15) Batch/Lot number: 1423 Purity: 99.6%

3. Soils/Sediment: The study was conducted using four European test soils and one U.S. soil (p. 16; Table 1, p. 29). Pesticide us e history at the collection sites and soil collection depths were not repor ted. The soils were stored at ca . 4  C. All soils were sieved (2-mm) and air-dried prior to use. A summary of the physical and chemical properties of the soils using US DA Soil Taxonomy is provided in Table 2 . The U.S. test soils were representative of use sites.

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# Table 2. Description of Soil/Sediment

Soil Name

Origin Speyer 2.2

Speyer Institute, Germany Pavia

Ottobiano, Italy Drummer

Rochelle, Illinois, USA Nambsheim

Nambsheim, France Vercelli

Bianze, Italy

USDA Textural Class Loamy sand Loamy sand Silt loam Sandy loam Silt loam

% Sand 69.2 78.4 16.8 57.2 20.4 % Silt 24.8 14.8 54.4 32.8 62.8 % Clay 6.0 6.8 28.8 10.0 16.8 %OC 2.1 0.5 3.1 0.7 1.2 CEC (meq/100 cc) 10.67 2.59 34.05 6.48 8.45 pH in water 6.4 5.2 5.5 7.8 5.8 % moisture (1/3 bar) Not reported Not reported Not reported Not reported Not reported

Water holding capacity (g/100 g) Not reported Not reported Not reported Not reported Not reported

Soil Taxonomy Not reported Not reported Not reported Not reported Not reported CaCO 3 

## (%) Not reported Not reported Not reported Not reported Not reported Data were obtained from p. 16 and Table 1, p. 29 of the study report.



# B. Study design

1. Experimental conditions: Preliminary pilot tests were conducted to determine the optimum soil:solution ratio and equilibrium times to be used in the definitive study and to determine the volume of s upernatant to be removed and added following equilibration (pp. 17-18). The appropr iate soil:solution ratio to be used in the definitive study was determined using duplicate aliquot s of soil (10 g dry weight) that were pre-equilibrated by shaking at ca . 20-22  C for ca . 18 hours in darkness with 0.01M CaCl 2 

# solution at a soil:solution ratio of 1:2 (w:v). The slurries were dosed with treatment solu tion to establish a no minal concentration of ca . 1 μg/mL. The samples were shaken for ca . 24 hours in darkness at ca . 20- 22  C, centrifuged, and the supernatants we re removed prior to analysis using LSC. The experiment was repeated for the Drummer soil at soil:solution ratios of 1:10 (w:v; 2 g dry weight equivalent of soil) and 1:20 (w:v; 1 g dry weight equivalent of soil) due to unacceptably high levels of test substance adsorption to the soil. Based on the results, a soil:solu tion ratio of 1:20 (w:v) was selected for the Drummer silt loam soil and 1:2 (w:v) was selected for the remaining test soils (p. 25).

The volume of supernatant to be remove d and added following equilibration was determined using triplicate samples of each soil (10 g dry weight equivalent) at soil:solution ratios of 1:2 (w:v), except for the Drummer soil where a 1:20 (w:v) ratio was used (p. 18). Aqueous 0.01M CaCl 2 

# solution was added to each sample to achieve a final volume of 20 mL. The samples were shaken for 24 hours in darkness at ca . 20-22  C, centrifuged, and the supernatants were removed and measured. A mean of the volumes removed for each soil type was calculated.

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

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# The equilibrium period to be used in th e definitive study was determined using six samples of each soil (10 g dry weight e quivalent) at soil:sol ution ratios of 1:2 (w:v), except for the Drummer soil for which a 1:20 (w:v) ratio was used (pp. 18- 19). Aqueous 0.01M CaCl 2 

# solution was added to each sample to achieve a final volume of 19 mL. The samples were pre-equilibrated by shaking for ca . 18 hours in darkness at ca . 20-22  C. The slurries were dosed with treatment solution to establish a nominal concentration of ca . 1 μg/mL. The samples were shaken in darkness at ca . 20-22  C. After 0.5, 2, 4, 8, 24, and 48 hours, the samples were centrifuged and the supernatants were rem oved prior to analysis using LSC. The 24-hour supernatants were extracted by shak ing once with acetonitrile, twice with acetonitrile:water (4:1, v:v), and once w ith acetonitrile:water (1:1, v:v). The extracts were analyzed using LSC. The remaining soils were air-dried and analyzed using LSC following combustion. Th e stability of the test substance was assessed by analyzing 24-hour samples using TLC. Adsorption of the test substance to the test containers was a ssessed by shaking test solutions at the highest and lowest test concentrations in tubes without soil for 24 hours at 20- 22  C and analyzing using LSC. Based on the results, an equilibrium time of 24 hours was selected for use in the definitive study (pp. 25-26). IN-J0290 was shown to be stable, accounting for 82.8-96.2% in both the supernatants and soil extracts (Appendix 3, Table 5, p. 60). No signi ficant adsorption of radioactivity to the glass surface of the test containers was observed (Appendix 3, Table 6, p. 60).

For the definitive study, duplicate aliquots of each soil were pre-equilibrated by shaking in the dark for ca . 18 hours at ca . 20-22  C with 0.01M CaCl 2 

# solution (pp. 20-21). The soil:solution ratios used in the study were 1:20 (w:v; Drummer silt loam only) and 1:2 (w:v). The sa mples were dosed with IN-J0290 at a nominal concentration range of 0.01 to 1.0 μg/mL. The samples were maintained
at ca . 20-22  C in the dark for 24 hours. Following centrifugation, aliquots of the supernatants were removed and subjected to solvent extraction. For desorption, an equivalent volume of fresh 0.01M aqueous CaCl 2 

# solution was added to each test vessel from the highest initial con centration only, and the samples were equilibrated by shaking fo r 24 hours in the dark at ca . 20-22  C. Following centrifugation, the desorption step wa s repeated. Following centrifugation, aliquots of the supernatants were rem oved and subjected to solvent extraction.

2. Analytical procedures: Radioactivity in the aqueous supernatants was determined by Liquid Scintillation Coun ting (LSC; pp. 20-21). Aqueous solutions were extracted by shaking once with aceton itrile, twice with acetonitrile:water (4:1, v:v), and once with acetonitrile:wate r (1:1, v:v; p. 19). The extracts were analyzed using LSC. Non-extractable re sidues were measured by combustion and LSC.

Representative extracts and supernatan ts were pooled (10% by volume) and analyzed by normal phase TLC (Merck Kieselgel 60 254F; 0.25 mm thickness) using plates that were developed in dichloromethane:methanol:ammonia

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# (180:30:1, v:v:v; p. 22). Ra dioactive areas were detected using a Fuji BAS 2000 Bioimaging analyzer.

Radioactivity in amounts of less than tw ice the background levels were generally considered to be below the Limit of Detection (LOD; p. 21).

II. Results and Discussion

A. Mass Balance: Overall recovery was determined only for soils that were treated at the highest test concentration. Rec overies were 97.0% of the applied for the Speyer 2.2 loamy sand, 100.6% for the Pavia loamy sand, 98.1% for the silt loam, 100.2% for the Nambsheim sandy loam, and 100.3% for the Vercelli silt loam (p. 27; Table 10, p. 38).

B. Transformation of Parent Compound: TLC analysis of high-dose aqueous supernatants and soil extracts showed that [ 14 

# C]IN-J0290 was stable following the adsorption phase, accounting for ≥ 93.3% of the radioactivity (pp. 26-27; Table 3, p. 31; Table 11, p. 39; Figures 1-5, pp. 45-49). Following the second desorption step, [ 14 

# C]IN-J0290 accounted for ≥ 89.1% of the radioactivity in the high-dose aqueous supernatants and soil extracts. “Other” unidentified radioactivity accounted for ≤ 10.9%.

C. Findings: Reported values were calculated us ing linear regression (Excel) and the equations and methods discussed in the calculations section.

Following the adsorption phase, equilibration concentrations in water were appropriate for all treated soils, rang ing from 0.0004-0.5991 μg/mL (Tables 5-9, pp. 33-37). Following the second desorption ste p, equilibration concentrations in water were determined only for soils treated at the highest concentration. The concentrations were appropriate for all treated soils, ranging from 0.0631-0.1031 μg/mL (Tables 12-16, pp. 40-44).

Percent adsorption was not reported. Percent desorption, as percen t of the adsorbed, ranged from 21 to 47% of the applied for all test soils (p. 28).

Coefficients of variation (CV) were 1.9 to 1.3 across all [ 14 

# C]-treated soils for adsorption K F 

# and K Foc 

# , respectively, and 2.2 and 2.2 across all [ 14 

# C]-treated soils for desorption K F 

# and K Foc 

# , respectively.

Table 3. Description of Adsorption Coefficients

Soil K d 

## (L/kg) K oc 

## K F 

## (L/kg) K Foc 

## Speyer 2.2 Loamy sand 1.4 66 1.2 59 Pavia Loamy sand 2.8 560 2.3 452 Drummer Silt loam 69.8 2251 45.2 1458 Nambsheim Sandy loam 0.9 135 0.9 122 Vercelli Silt loam 3.0 247 2.4 197

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

10 of 31 Data were obtained from DER Attachment.



# Table 4. Description of De sorption Coefficients

Soil K des 

## (L/kg) K oc-des 

## K Fdes 

## (L/kg) K Foc-des 

## Speyer 2.2 Loamy sand 5.3 253 0.002 0 Pavia Loamy sand 7.7 1547 680.6 136117 Drummer Silt loam 160.2 5168 0.799 26 Nambsheim Sandy loam 3.0 434 7.455 1065 Vercelli Silt loam 7.5 625 0.662 55 

## Data were obtained from DER Attachment.



# Reviewer-calculated Freundlich adsorption coefficients were in agreement with those reported by the study author (Table 4, p. 32). Reviewer-calculated Freundlich desorption coefficients varied from those reported by the study author (Tables 12-16, pp. 40-44). These values were calculated usi ng only soils treated at the highest test concentration.

y = 1.3925x r2 = 0.9926

0.0 0.2 0.4 0.6 0.8

0.0 0.1 0.2 0.3 0.4 0.5 0.6IN-J0290 Concentration in Speyer 2.2 LoamySand- Adsorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

11 of 31 y = 2.7997x r2 = 0.9928

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4IN-J0290 Concentration in Pavia LoamySand- Adsorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

y = 69.77x r2 = 0.954

0 5 10 15

0.00 0.05 0.10 0.15 0.20 0.25IN-J0290 Concentration in Drummer SiltLoam- Adsorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

12 of 31 y = 0.9423x r2 = 0.9738

0.0 0.1 0.2 0.3 0.4 0.5 0.6

0.0 0.1 0.2 0.3 0.4 0.5 0.6IN-J0290 Concentration in Nambsheim SandyLoam- Adsorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)



### y = 2.964x r2 = 0.9955

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4IN-J0290 Concentration in Vercelli Silt LoamAdsorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

13 of 31 y = 5.3138x r2 = -1.036

0.0 0.1 0.2 0.3 0.4 0.5

0.00 0.02 0.04 0.06 0.08 0.10IIN-J0290 Concentration in Speyer 2.2Loamy Sand- Desorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)



### y = 7.7327x r2 = 0.5765

0.0 0.2 0.4 0.6 0.8 1.0

0.00 0.05 0.10 0.15 0.20IN-J0290 Concentration in Pavia LoamySand- Desorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

14 of 31 y = 160.22x r2 = -3.297

0 5 10 15

0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08IN-J0290 Concentration in Drummer SiltLoam- Desorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)



### y = 3.0367x r2 = 0.9274

0.0 0.1 0.2 0.3

0.00 0.02 0.04 0.06 0.08 0.10IN-J0290 Concentration in NambsheimSandy Loam- Desorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

15 of 31 y = 7.5051x r2 = -277.7

0.0 0.2 0.4 0.6 0.8

0.00 0.05 0.10 0.15IN-J0290 Concentration in Vercelli SiltLoam- Desorption Phase (mg/kg)



### Pesticide Concentration in Water at Equilibrium (mg/L)



# The results of the regression of K d 

# by percent organic carbo n, CEC, and percent clay are tabulated below:

Table 5. Summary of Regressed Kd vs %OC, CEC, and %Clay
Parameter r 2 

## p-value Kd vs. % organic carbon 0.664 0.093 Kd vs. % clay 0.807 0.038 Kd vs. CEC 0.937 0.007



# The data indicate a significan t correlation betwee n the adsorption of IN-J0290 to soil and cation exchange capacity (r 2 

# = 0.937) and percent clay (r 2 

# = 0.807). The correlation between adsorption of IN-J0290 to soil and percent organic carbon was less pronounced (r 2 

# = 0.664; DER Attachment).

y = 0.029x + 1.0688 r2 = 0.6637

0 1 2 3 4

0 25 50 75Percent Organic carbon



### Kd (L/kg)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

16 of 31 y = 0.2805x + 9.3115 r2 = 0.8072

0 10 20 30

0 25 50 75Percent Clay



### Kd (L/kg)



### y = 0.3972x + 6.2618 r2 = 0.9373

0 10 20 30

0 25 50 75Cation Exchange Capacity (meq/100g)



### Kd (L/kg)



# III. Study Deficiencies and Reviewer’s Comments

1. The study was conducted using a transforma tion product rather than the parent compound. 2. The study was conducted using only three t ypes of soil (loamy sand, silt loam, and sandy loam). OCSPP guidelines recomme nd that at least 5 soils/sediments of different textures are examined. 3. Mass balances were determined for soils tr eated at the highest test concentration, rather across all test concentrations. 4. The desorption phase of the experiment was conducted using the highest test concentration only, rather than all five test concentrations. The reviewer determined desorption K values based on th e highest test concentration desorption data; however, these values are of limited value since only one test concentration was employed. As with adsorption kine tics/adsorption isotherms, desorption

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# kinetics/desorption isotherms should be conducted using five test substance concentrations, covering prefer ably two orders of magnitude.

IV. References

1. 

# U.S. Environmental Protection Agency. 2008. Fate, Transport and Transformation Test Guidelines, OPPTS 835.1230, adsorption/de sorption (batch equilibrium). Office of Prevention, Pesticides and Toxic S ubstances, Washington, DC. EPA 712-C-08- 019.IN-J0290 (PC 129008 for Nicosulfuron) MRID 4916970118 of 31DER ATTACHMENT 1. IN-J0290 and Its Environmental Transformation Products.ACode Name/SynonymChemical NameChemical StructureStudyTypeMRIDMaximum%AR (day)Final %AR(study length)PARENTIN-J0290IUPAC:4,6-Dimethoxy-pyrimidin2-ylamineCAS:2-Amino-4,6-dimethoxypyrimidineCAS No.:36315-01-2Formula:C6H9N3O2MW:155.15 g/molSMILES:Nc1nc(cc(n1)OC)OC835.1230Batchequilibrium49169701NANAMAJOR (>10%) TRANSFORMATION PRODUCTSNo major transformationproducts were identified.MINOR (<10%) TRANSFORMATION PRODUCTSNo minor transformationproducts were identified.REFERENCE COMPOUNDS NOT IDENTIFIEDAll compounds used as reference compounds were identified.AAR means “applied radioactivity”. MW means“molecular weight”. NA means "not applicable".

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# Attachment 2: Calculations

Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption K d values

1 0.5131 0.6761 1.32 1 0.4994 0.7033 1.41 0.5 0.2265 0.3492 1.54 0.5 0.2289 0.3445 1.51 0.1 0.0383 0.0716 1.87 0.1 0.0365 0.0751 2.06 0.05 0.0156 0.0409 2.62 0.05 0.0162 0.0397 2.45 0.01 0.0036 0.0093 2.58 0.01 0.0035 0.0095 2.71 Data were obtained from Table 5, p. 33 of the study report. SUMMARY OUTPUT

Regression Statistics Multiple R 0.997939891 R Square 0.995884027 Adjusted R Square 0.884772915 Standard Error 0.023490303 Observations 10

ANOVA df SS MS F Significance F Regression 1 1.201589051 1.201589051 2177.60304 4.91552E-11 Residual 9 0.004966149 0.000551794 Total 10 1.2065552

Coefficients Standard Error t Stat P-value Low er 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0 #N/A #N/A #N/A #N/A #N/A #N/A #N/A X Variable 1 1.392474915 0.029839945 46.66479446 4.7713E-12 1.324972269 1.459977561 1.324972269 1.459977561 Speyer 2.2 Loamy sand

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Concen in soil at ads equil (Cs) (μg/g) Kd

y = 1.3925x r2 = 0.9926

0.0 0.2 0.4 0.6 0.8

0.0 0.1 0.2 0.3 0.4 0.5 0.6IN-J0290 Concentration in Speyer 2.2Loamy Sand- Adsorption Phase (mg/kg)

Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

20 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption K d 

### values

1 0.3651 1.019 2.79 1 0.3719 1.005 2.70 0.5 0.1654 0.4937 2.98 0.5 0.1629 0.4986 3.06 0.1 0.0223 0.1078 4.83 0.1 0.0219 0.1085 4.95 0.05 0.0093 0.0556 5.98 0.05 0.0089 0.0564 6.34 0.01 0.0019 0.0132 6.95 0.01 0.0019 0.0133 7.00 Data were obtained from Table 6, p. 34 of the study report. SUMMARY OUTPUT

Regression Statistics Multiple R 0.997986181 R Square 0.995976418 Adjusted R Square 0.884865307 Standard Error 0.033901163 Observations 10

ANOVA df SS MS F Significance F Regression 1 2.56040059 2.56040059 2227.81297 4.48847E-11 Residual 9 0.0103436 0.001149289 Total 10 2.57074419

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0 #N/A #N/A #N/A #N/A #N/A #N/A #N/A X Variable 1 2.799702961 0.059316101 47.19971368 4.3078E-12 2.66552062 2.933885303 2.66552062 2.933885303 Pavia Loamy sand

Initial soln concen (C o 

### ) (mg/L) Concen in soln at ads equil (C eq 

### )

(μg/mL) Concen in soil at ads equil (Cs) (μg/g) Kd

y = 2.7997x r2 = 0.9928

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4IN-J0290 Concentration in Pavia LoamySand- Adsorption Phase (mg/kg)

Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

21 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption K d values

1 0.1898 13.1 69.02 1 0.2018 12.87 63.78 0.5 0.0671 6.616 98.60 0.5 0.0676 6.607 97.74 0.1 0.0061 1.347 220.82 0.1 0.0061 1.334 218.69 0.05 0.0022 0.6706 304.82 0.05 0.0021 0.6663 317.29 0.01 0.0005 0.1538 307.60 0.01 0.0004 0.1565 391.25 Data were obtained from Table 7, p. 35 of the study report. SUMMARY OUTPUT

Regression Statistics Multiple R 0.987052925 R Square 0.974273478 Adjusted R Square 0.863162366 Standard Error 1.107649795 Observations 10

ANOVA df SS MS F Significance F Regression 1 418.1645841 418.1645841 340.833524 7.63429E-08 Residual 9 11.04199261 1.226888067 Total 10 429.2065767

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0 #N/A #N/A #N/A #N/A #N/A #N/A #N/A X Variable 1 69.76990294 3.779174678 18.46167718 1.837E-08 61.22081587 78.31899 61.22081587 78.31899 Drummer Silt loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Concen in soil at ads equil (Cs) (μg/g) Kd

y = 69.77x r2 = 0.954

0 5 10 15

0.00 0.05 0.10 0.15 0.20 0.25IN-J0290 Concentration in Drummer SiltLoam- Adsorption Phase (mg/kg)

Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

22 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption K d values

1 0.5966 0.5395 0.90 1 0.5991 0.5347 0.89 0.5 0.2601 0.2944 1.13 0.5 0.2568 0.301 1.17 0.1 0.0391 0.0716 1.83 0.1 0.0397 0.0705 1.78 0.05 0.0175 0.0378 2.16 0.05 0.0176 0.0377 2.14 0.01 0.0036 0.0094 2.61 0.01 0.0037 0.0093 2.51 Data were obtained from Table 8, p. 36 of the study report. SUMMARY OUTPUT

Regression Statistics Multiple R 0.993074883 R Square 0.986197723 Adjusted R Square 0.875086612 Standard Error 0.034304672 Observations 10

ANOV A df SS MS F Significance F Regression 1 0.756767196 0.756767196 643.066322 6.26416E-09 Residual 9 0.010591294 0.00117681 Total 10 0.76735849

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0 #N/A #N/A #N/A #N/A #N/A #N/A #N/A X Variable 1 0.94234699 0.037160621 25.35875238 1.1092E-09 0.858283825 1.026410156 0.858283825 1.026410156 Nambsheim Sandy loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Concen in soil at ads equil (Cs)

(μg/g) Kd

y = 0.9423x r2 = 0.9738

0.0 0.1 0.2 0.3 0.4 0.5 0.6

0.0 0.1 0.2 0.3 0.4 0.5 0.6IN-J0290 Concentration in NambsheimSandy Loam- Adsorption Phase (mg/kg)

Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

23 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption K d values

1 0.3509 1.029 2.93 1 0.3528 1.025 2.91 0.5 0.1588 0.4975 3.13 0.5 0.1583 0.4985 3.15 0.1 0.0216 0.1068 4.94 0.1 0.0227 0.1046 4.61 0.05 0.0096 0.0538 5.60 0.05 0.0099 0.0533 5.38 0.01 0.0018 0.0131 7.28 0.01 0.0018 0.013 7.22 Data were obtained from Table 9, p. 37 of the study report. SUMMARY OUTPUT

Regression Statistics Multiple R 0.998729904 R Square 0.997461422 Adjusted R Square 0.886350311 Standard Error 0.027256712 Observations 10

ANOV A df SS MS F Significance F Regression 1 2.627211485 2.627211485 3536.29194 7.10383E-12 Residual 9 0.006686355 0.000742928 Total 10 2.63389784

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0 #N/A #N/A #N/A #N/A #N/A #N/A #N/A X Variable 1 2.963965108 0.04984241 59.46672966 5.4186E-13 2.851213743 3.076716472 2.851213743 3.076716472 Vercelli Silt loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Concen in soil at ads equil (Cs) (μg/g) Kd

y = 2.964x r2 = 0.9955

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4IN-J0290 Concentration in Vercelli SiltLoam- Adsorption Phase (mg/kg)

Pesticide Concentration in Water at Equilibrium (mg/L)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

24 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption KF values

1 0.5131 -0.290 0.6761 -0.169989064 1 0.4994 -0.302 0.7033 -0.152859383 0.5 0.2265 -0.645 0.3492 -0.456925765 0.5 0.2289 -0.640 0.3445 -0.462810774 0.1 0.0383 -1.417 0.0716 -1.145086978 0.1 0.0365 -1.438 0.0751 -1.124360063 0.05 0.0156 -1.807 0.0409 -1.388276692 0.05 0.0162 -1.790 0.0397 -1.401209493 0.01 0.0036 -2.444 0.0093 -2.031517051 0.01 0.0035 -2.456 0.0095 -2.022276395 Data were obtained from Table 5, p. 33 of the study report. SUMMARY OUTPUT SE Regression Statistics KF: 1.247118411 1.046777069 Multiple R 0.999086036 1/n: 0.855327657 0.012937935 R Square 0.998172907 Adjusted R Square 0.99794452 Standard Error 0.031825156 Observations 10

ANOV A df SS MS F Significance F Regression 1 4.426659865 4.426659865 4370.539846 3.04943E-12 Residual 8 0.008102724 0.001012841 Total 9 4.43476259

Coefficients Standard Error t Stat P-valu e Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0.095907691 0.0198542 4.830599583 0.001303636 0.050123823 0.14169156 0.050123823 0.141691558 X Variable 1 0.855327657 0.012937935 66.11005859 3.04943E-12 0.825492725 0.88516259 0.825492725 0.885162589 Speyer 2.2 Loamy sand

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Log C eq (ug/mL) Concen in soil at ads equil (Cs)

(μg/g) Log C s (μg/g)

y = 1.2471x 0.8553 r2 = 0.9982

0.0 0.2 0.4 0.6 0.8

0.0 0.1 0.2 0.3 0.4 0.5 0.6Cs

Ceq

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

25 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption KF values

1 0.3651 -0.438 1.019 0.008174184 1 0.3719 -0.430 1.005 0.002166062 0.5 0.1654 -0.781 0.4937 -0.306536873 0.5 0.1629 -0.788 0.4986 -0.302247726 0.1 0.0223 -1.652 0.1078 -0.967381239 0.1 0.0219 -1.660 0.1085 -0.964570262 0.05 0.0093 -2.032 0.0556 -1.254925208 0.05 0.0089 -2.051 0.0564 -1.248720896 0.01 0.0019 -2.721 0.0132 -1.879426069 0.01 0.0019 -2.721 0.0133 -1.876148359 Data were obtained from Table 6, p. 34 of the study report. SUMMARY OUTPUT SE Regression Statistics KF: 2.261076321 1.049822163 Multiple R 0.999096529 1/n: 0.807510691 0.012144224 R Square 0.998193875 Adjusted R Square 0.997968109 Standard Error 0.031917052 Observations 10

ANOVA df SS MS F Significance F Regression 1 4.504043264 4.504043264 4421.371428 2.91181E-12 Residual 8 0.008149586 0.001018698 Total 9 4.51219285

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0.354315222 0.021115737 16.7796757 1.61115E-07 0.305622245 0.4030082 0.305622245 0.403008199 X Variable 1 0.807510691 0.012144224 66.49339387 2.91181E-12 0.779506059 0.83551532 0.779506059 0.835515322 Pavia Loamy sand

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Log C eq (ug/mL) Concen in soil at ads equil (Cs) (μg/g) Log C s (μg/g)

y = 2.2611x 0.8075 r2 = 0.9982

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4Cs

Ceq

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

26 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption KF values

1 0.1898 -0.722 13.1 1.117271296 1 0.2018 -0.695 12.87 1.109578547 0.5 0.0671 -1.173 6.616 0.820595497 0.5 0.0676 -1.170 6.607 0.820004307 0.1 0.0061 -2.215 1.347 0.129367596 0.1 0.0061 -2.215 1.334 0.12515583 0.05 0.0022 -2.658 0.6706 -0.173536451 0.05 0.0021 -2.678 0.6663 -0.176330187 0.01 0.0005 -3.301 0.1538 -0.813043665 0.01 0.0004 -3.398 0.1565 -0.805485658 Data were obtained from Table 7, p. 35 of the study report. SUMMARY OUTPUT SE Regression Statistics KF: 45.20571569 1.114996633 Multiple R 0.996507728 1/n: 0.711951728 0.021091838 R Square 0.993027651 Adjusted R Square 0.992156108 Standard Error 0.064442421 Observations 10

ANOVA df SS MS F Significance F Regression 1 4.731686027 4.731686027 1139.389526 6.48021E-10 Residual 8 0.033222605 0.004152826 Total 9 4.764908632

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 1.655193349 0.047273556 35.01309181 4.844E-10 1.546180334 1.76420636 1.546180334 1.764206364 X Variable 1 0.711951728 0.021091838 33.75484448 6.48021E-10 0.663313861 0.76058959 0.663313861 0.760589595

Chemical: IN-J0290 Drummer Silt loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Log C eq (ug/mL) Concen in soil at ads equil (Cs) (μg/g) Log C s (μg/g)

y = 45.206x 0.712 r2 = 0.993

0 5 10 15

0.00 0.05 0.10 0.15 0.20 0.25Cs

Ceq

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

27 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption KF values

1 0.5966 -0.224 0.5395 -0.268008551 1 0.5991 -0.223 0.5347 -0.271889816 0.5 0.2601 -0.585 0.2944 -0.531062194 0.5 0.2568 -0.590 0.301 -0.521433504 0.1 0.0391 -1.408 0.0716 -1.145086978 0.1 0.0397 -1.401 0.0705 -1.151810883 0.05 0.0175 -1.757 0.0378 -1.4225082 0.05 0.0176 -1.754 0.0377 -1.42365865 0.01 0.0036 -2.444 0.0094 -2.026872146 0.01 0.0037 -2.432 0.0093 -2.031517051 Data were obtained from Table 8, p. 36 of the study report. SUMMARY OUTPUT SE Regression Statistics KF: 0.856961664 1.044212136 Multiple R 0.999007976 1/n: 0.789781139 0.012446896 R Square 0.998016937 Adjusted R Square 0.997769054 Standard Error 0.031379988 Observations 10

ANOVA df SS MS F Significance F Regression 1 3.964577827 3.964577827 4026.163515 4.23203E-12 Residual 8 0.007877629 0.000984704 Total 9 3.972455456

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept -0.067038606 0.018788736 -3.568021023 0.007314726 -0.110365509 -0.0237117 -0.11036551 -0.023711702 X Variable 1 0.789781139 0.012446896 63.45205682 4.23203E-12 0.761078546 0.81848373 0.761078546 0.818483732 Nambsheim Sandy loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Log C eq (ug/mL) Concen in soil at ads equil (Cs) (μg/g) Log C s (μg/g)

y = 0.857x 0.7898 r2 = 0.998

0.0 0.1 0.2 0.3 0.4 0.5 0.6

0.0 0.1 0.2 0.3 0.4 0.5 0.6Cs

Ceq

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

28 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Reviewer-calculated Adsorption KF values

1 0.3509 -0.455 1.029 0.012415375 1 0.3528 -0.452 1.025 0.010723865 0.5 0.1588 -0.799 0.4975 -0.303206915 0.5 0.1583 -0.801 0.4985 -0.302334837 0.1 0.0216 -1.666 0.1068 -0.971428747 0.1 0.0227 -1.644 0.1046 -0.980468315 0.05 0.0096 -2.018 0.0538 -1.269217724 0.05 0.0099 -2.004 0.0533 -1.273272791 0.01 0.0018 -2.745 0.0131 -1.882728704 0.01 0.0018 -2.745 0.013 -1.886056648 Data were obtained from Table 9, p. 37 of the study report. SUMMARY OUTPUT SE Regression Statistics KF: 2.360487636 1.022999507 Multiple R 0.999808934 1/n: 0.820431351 0.005671082 R Square 0.999617905 Adjusted R Square 0.999570143 Standard Error 0.014819531 Observations 10

ANOVA df SS MS F Significance F Regression 1 4.596438705 4.596438705 20929.19765 5.82922E-15 Residual 8 0.001756948 0.000219618 Total 9 4.598195653

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 0.37300173 0.009875424 37.77070401 2.64993E-10 0.350228961 0.3957745 0.350228961 0.395774499 X Variable 1 0.820431351 0.005671082 144.6692699 5.82922E-15 0.807353811 0.83350889 0.807353811 0.83350889 Vercelli Silt loam

Initial soln concen (C o ) (mg/L) Concen in soln at ads equil (C eq )

(μg/mL) Log C eq (ug/mL) Concen in soil at ads equil (Cs) (μg/g) Log C s (μg/g)

y = 2.3605x 0.8204 r2 = 0.9996

0.0 0.3 0.6 0.9 1.2

0.0 0.1 0.2 0.3 0.4Cs

Ceq

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

29 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Soil Regressed K d % organic carbon Spe y er 2.2 Loam y sand 1.39 2.1 Pavia Loam y sand 2.80 0.5 Drummer Silt loam 69.77 3.1 Nambsheim Sandy loam 0.94 0.7

Vercelli Silt loam 2

. 96 1

. 2

SUMMARY OUTPUT

Regression Statistics Multiple R 0.814663294 R Square 0.663676283 Adjusted R Square 0.551568378 Standard Error 0.721857011 Observations 5

ANOVA df SS MS F Significance F Regression 1 3.084767365 3.084767365 5.919977544 0.093071791 Residual 3 1.563232635 0.521077545 Total 4 4.648

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 1.068770145 0.372302226 2.870705765 0.064013353 -0.116061697 2.253601988 -0.116061697 2.253601988 X Variable 1 0.028973877 0.011908213 2.433100397 0.093071791 -0.008923371 0.066871125 -0.008923371 0.066871125 Data were obtained from Table 1, p. 29 of the study report. The reviewer calculated Kd values using data obtained from Table 5-9, pp. 33-37 of the study report. y = 0.029x + 1.0688 r2 = 0.6637

0 1 2 3 4

0 25 50 75Percent Organic carbon

Kd (L/kg)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

30 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Soil Regressed K d 

### % clay Speyer 2.2 Loamy sand 1.39 6

Pavia Loamy sand 2.80 6.8 Drummer Silt loam 69.77 28.8 Nambsheim Sandy loam 0.94 10

Vercelli Silt loam 2

. 96 16.8

SUMMARY OUTPUT

Regression Statistics Multiple R 0.898418729 R Square 0.807156213 Adjusted R Square 0.74287495 Standard Error 4.798547954 Observations 5

ANOV A df SS MS F Significance F Regression 1 289.1298126 289.1298126 12.55663286 0.038266943 Residual 3 69.07818739 23.02606246 Total 4 358.208

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 9.311492812 2.474880834 3.762400469 0.032836032 1.435317444 17.18766818 1.435317444 17.18766818 X Variable 1 0.280505801 0.079159902 3.543533951 0.038266943 0.028583663 0.53242794 0.028583663 0.53242794 Data were obtained from Table 1, p. 29 of the study report. The reviewer calculated Kd values us ing data obtained from Table 5-9, pp. 33-37 of the study report. y = 0.2805x + 9.3115 r2 = 0.8072

0 10 20 30

0 25 50 75Percent Clay

Kd (L/kg)

## IN-J0290 (PC 129008 for Nicosulfuron) MRID 49169701

31 of 31 Chemical: IN-J0290 PC Code: 129008 MRID: 49169701 Guideline No: 835.1230

Soil Regressed K d 

### CEC Speyer 2.2 Loamy sand 1.39 10.67

Pavia Loam y sand 2.80 2.59 Drummer Silt loam 69.77 34.05 Nambsheim Sandy loam 0.94 6.48 Vercelli Silt loam 2

. 96 8.45

SUMMARY OUTPUT

Regression Statistics Multiple R 0.968131271 R Square 0.937278157 Adjusted R Square 0.916370876 Standard Error 3.596249649 Observations 5

ANOVA df SS MS F Significance F Regression 1 579.7898454 579.7898454 44.83022719 0.006796635 Residual 3 38.79903461 12.93301154 Total 4 618.58888

Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0% Intercept 6.261829015 1.854788035 3.376034833 0.04321768 0.359065685 12.16459235 0.359065685 12.16459235 X Variable 1 0.397219639 0.059326024 6.695537856 0.006796635 0.208417753 0.586021525 0.208417753 0.586021525 Data were obtained from Table 1, p. 29 of the study report. The reviewer calculated Kd values using data obtained from Table 5-9, pp. 33-37 of the study report. y = 0.3972x + 6.2618 r2 = 0.9373

0 10 20 30

0 25 50 75Cation Exchange Capacity(meq/100 g)



### Kd (L/kg)