# Patent

## Title
Compounds, pharmaceutical compositions and uses thereof

## Abstract

The present invention relates to compounds of general formula I,




wherein the groups R1, R2, and A are as defined in the application, which have valuable pharmacological properties, and in particular bind to the GPR119 receptor and modulate its activity.

## Background
FIELD OF THE INVENTION
The present invention relates to new compounds of formula I




wherein the groups R1, R2, and A are defined as hereinafter, to processes for preparing such compounds, to pharmaceutical compositions, to their use as modulators of the G-protein-coupled receptor GPR119, to methods for their therapeutic use, in particular in diseases and conditions mediated by the modulation of the G-protein-coupled receptor GPR119, and to pharmaceutical compositions comprising them.

BACKGROUND OF THE INVENTION
Diabetes mellitus is a serious metabolic disease which affects more than 100 million people worldwide. In the USA there are more than 12 million diabetics with 600,000 new cases diagnosed every year. The prevalence of diabetes mellitus is increasing, resulting in a high frequency of complications and a substantial impairment of quality of life and life expectancy. Because of diabetes-associated microvascular complications, in the industrialised countries type 2 diabetes is currently the most common cause of adult-onset loss of vision, renal insufficiency and amputations. In addition, type 2 diabetes is associated with a two- to five-fold increase in the risk of cardiovascular disease.
The UKPDS study (United Kingdom Prospective Diabetes Study) showed that intensive treatment with common therapeutic agents, e.g. metformin, sulphonylureas or insulin, results in only a limited improvement in glycaemic control (difference in the HbA1c value  ̃0.9%). Moreover, glycaemic control deteriorated considerably over time even in patients in the intensive treatment group, and this was put down to a deterioration in beta cell function. Diabetes is also a major cause of damage to the retina at the back of the eye and increases the risk of cataract and glaucoma. Finally, diabetes is associated with nerve damage, particularly in legs and feet, which affects the patient's ability to feel pain and contributes to serious infections. All in all, complications of diabetes are one of the major causes of death worldwide.
Adiposity (obesity) is the result of an imbalance between calorie intake and energy consumption. It correlates to a high degree with insulin resistance and diabetes. However, the molecular mechanisms that are involved in obesity/diabetes syndromes are not yet clear. At an early stage of the development of obesity, an increased insulin secretion balances out the insulin resistance and protects the patient from hyperglycaemia. However, after a time, the beta cell function worsens and non-insulin-dependent diabetes develops in about 20% of the obese population. Obesity has thus become a critical risk factor for diabetes, but the factors that predispose one group of patients to a pathological change in insulin secretion as a response to the accumulation of fat are currently unknown. Obesity also significantly increases the risk of the development of cardiovascular disease. Diabetes is also implicated in the formation of kidney complaints, eye complaints and problems of the nervous system. Kidney disease, also known as nephropathy, sets in when the filtering mechanism of the kidneys is disrupted and proteins escape into the urine in excessive amounts and finally the kidney fails. Therefore there is a medical need for medicaments for preventing and/or treating metabolic disorders (particularly diabetes, predominantly type 2 diabetes) and the complications thereof. In particular there is a need for medicaments with good activity in terms of glycaemic control, disease-modifying properties and reducing cardiovascular morbidity and mortality, and which also have a better safety profile.
Dyslipidemia is a disorder of lipoprotein metabolism, including lipoprotein overproduction or deficiency. Dyslipidemias may be manifested by elevation of the total cholesterol, LDL cholesterol and triglyceride and free fatty acid concentrations, and a decrease in high-density lipoprotein (HDL) cholesterol concentration in the blood. Dyslipidemia occurs often in situations including diabetes, a common cause of lipidemia. For adults with diabetes, it has been recommended that the levels of LDL, HDL, and total cholesterol, and triglyceride be measured every year. Optimal LDL cholesterol levels for adults with diabetes are less than 100 mg/dL (2.60 mmol/L), optimal HDL cholesterol levels are equal to or greater than 40 mg/dL (1.02 mmol/L), and desirable triglyceride levels are less than 150 mg/dL (1.7 mmol/L).
GPR119 is a G-protein coupled receptor (also known as GPCR2, RUP3, SNORF25 or GDIR) which is expressed predominantly in the beta cells of the pancreas and in the K- and L-cells of the intestine. The GPR119 receptor and isoforms have been identified in mammalian species including human, rat, mouse, hamster, chimpanzee, rhesus monkey, cattle and dog. The expression of GPR119 in the pancreas and particularly in the pancreatic β-cells led to the hypothesis that the GPR119 receptor could have effects upon insulin secretion. Activation of the receptor stimulates the cAMP signal pathway, increasing the intracellular levels of cAMP in these cells. This will lead to an improved diabetic situation by a dual action of such a compound: stimulation of cAMP in the beta cell occurs directly via activation of GPR119 in these cells and furthermore indirectly via stimulation of the release of neuroendocrine peptides like GIP and GLP-1 and PYY from the gut. The release of these peptides may have also additional beneficial effects, e.g. on food intake, gastric emptying and other yet unknown functions. Also, a GPR119 agonist can be expected to bring about an improvement in the beta cell function and the beta cell mass. In fact, activation of GPR119 stimulates insulin secretion in-vitro and in-vivo (in rodents) in a glucose-dependent manner. The discovery of two endogenous ligands, lysophospha-tidylcholine (LPC) and oleoylethanolamide (OEA) as well as more potent GPR119 agonists have led to the characterization of GPR119 as both an insulin and incretin (GLP-1 and GIP) secretagogue receptor capable of lowering plasma glucose and thereby facilitating glycemic control without the risk of hypoglycemia (Biochem. Biophys. Res. Comm. 2005, 744-751; Cell Metabolism 2006, 167-175; Endocrinolgy 2007, 2601-9). It has recently been shown that GPR119 agonists effectively lower the blood glucose levels in diabetic rodents without the risk of hypoglycaemia. GPR119 knockout animals have shown that both insulin and incretin secretion induced by GPR119 agonists are dependent upon GPR119 receptor. In addition, it has been shown that GPR119 agonists decrease food intake resulting in weight loss in Sprague Dawley rats. Therefore the GPR119 agonists may be expected to have a therapeutic benefit in metabolic diseases. Examples of such diseases include type 1 diabetes, type 2 diabetes, insufficient glucose tolerance, insulin resistance, hyperglycaemia, hyperlipidaemia, hypercholesterolaemia, dyslipidaemia, syndrome X, metabolic syndrome, obesity, high blood pressure, chronic systemic inflammation, retinopathy, neuropathy, nephropathy, atherosclerosis, endothelial dysfunction and bone-related diseases (such as osteoporosis, rheumatoid arthritis or osteoarthritis). For comparison and additional information also see

    1. Dhayal, S., Morgan, N. G. The significance of GPR119 agonists as a future treatment for type 2 diabetes. Drug News Perspect. 2010, 23(7), 418-24.
    2. Yoshida, S., Tanaka, H., Oshima, H., Yamazaki, T., Yonetoku, Y., Ohishi, T., Matsui, T., Shibasaki, M. AS1907417, a novel GPR119 agonist, as an insulinotropic and β-cell preservative agent for the treatment of type 2 diabetes. Biochem Biophys Res Commun. 2010, 400(4), 745-51.
    3. Jones, R. M., Leonard, J. N., Buzard, D. J., Lehman, J. GPR119 agonists for the treatment of type 2 diabetes. Expert Opinion on Therapeutic Patents 2009, Vol. 19, No. 10: 1339-1359.


AIM OF THE PRESENT INVENTION
The aim of the present invention is to provide new compounds, in particular new furo[2,3-c]pyridine derivatives, which are active with regard to the G-protein-coupled receptor GPR119.
Another aim of the present invention is to provide new compounds, in particular new furo[2,3-c]pyridine derivatives, which are agonists of the G-protein-coupled receptor GPR119.
A further aim of the present invention is to provide new compounds, in particular new furo[2,3-c]pyridine derivatives, which have an activating effect on the G-protein-coupled receptor GPR119 in vitro and/or in vivo and possess suitable pharmacological and pharmacokinetic properties to use them as medicaments.
A further aim of the present invention is to provide effective GPR119 agonists, in particular for the treatment of metabolic disorders, for example diabetes, dyslipidemia and/or obesity.
A further aim of the present invention is to provide methods for treating a disease or condition mediated by the activation of the G-protein-coupled receptor GPR119 in a patient.
A further aim of the present invention is to provide a pharmaceutical composition comprising at least one compound according to the invention.
A further aim of the present invention is to provide a combination of at least one compound according to the invention with one or more additional therapeutic agents.
A further aim of the present invention is to provide methods for the synthesis of the new compounds, in particular furo[2,3-c]pyridine derivatives.
A further aim of the present invention is to provide starting and/or intermediate compounds suitable in methods for the synthesis of the new compounds.
Further aims of the present invention become apparent to those skilled in the art by the description hereinbefore and in the following and by the examples.
OBJECT OF THE INVENTION
It has now been found that the compounds according to the invention described in more detail hereinafter have surprising and particularly advantageous properties, and in particular as GPR119 agonists.
In a first aspect the invention thus relates to a compound of formula I




wherein

    R1 is selected from the group R1-G1 consisting of a 5- or 6-membered heteroaromatic ring which contains 1, 2, or 3 heteroatoms independently of each other selected from N, O, and S, and which may be optionally substituted with a group Rc and/or one or more substituents independently selected from LA; and
    
        a group —C(═O)—O—Ra, wherein Ra denotes C1-6-alkyl optionally mono- or polysubstituted with fluorine; or C3-6-cycloalkyl optionally mono- or polysubstituted with fluorine and optionally substituted with a group selected from CH3, CF3, and CHF2; and
        a group —CH2—Rb, wherein Rb denotes C1-6-alkyl optionally mono- or polysubstituted with fluorine; or C3-6-cycloalkyl optionally mono- or polysubstituted with fluorine and optionally substituted with a group selected from CH3, CF3, and CHF2;
    
    
    R2 is selected from the group R2-G1 consisting of H and C1-3-alkyl; and
    A is selected from the group A-G1 consisting of 1,2,3,6-tetrahydropyridin-4-yl and piperazin-1-yl, each of which substituted at the N with a C1-4-alkyl-S(═O)2— group; and
    
        a phenyl ring and a 5- or 6-membered heteroaromatic ring which contains 1, 2 or 3 heteroatoms independently of each other selected from N, O and S, each of which may be substituted with a group T and may be optionally substituted with one or more substituents independently selected from LA;
    
    
    T is selected from the group T-G1 consisting of F, Cl, Br, I, CN, OH, NO2, C1-6-alkyl, C2-6-alkenyl, C2-6-alkynyl, C3-6-cycloalkyl, C1-6-alkyl-O—, C3-6-cycloalkyl-O—, C1-6-alkyl-S—, HO—C(═O)—, C1-6-alkyl-O—C(═O)—, C1-4-alkyl-C(═O)—, C3-6-cycloalkyl-C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—(RN)N—, heterocyclyl, heterocyclyl-O—, aryl, aryl-O—, heteroaryl and heteroaryl-O—,
    
        wherein each alkyl, alkenyl, alkynyl, and cycloalkyl group may be optionally substituted with one or more substituents independently of each other selected from F, Cl, CN, OH, C1-3-alkyl, C3-6-cycloalkyl, C1-3alkyl-O—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, aryl, heteroaryl, and heterocyclyl, and
        wherein aryl denotes phenyl or naphthyl, and
        wherein heteroaryl is a 5- or 6-membered aromatic ring which contains 1, 2, 3 or 4 heteroatoms independently of each other selected from N, O and S, wherein the H-atom in one or more NH groups may be optionally replaced by RN; and
        wherein heterocyclyl is a 4- to 7-membered unsaturated or saturated other are replaced by NRN, O, —C(═O)—, S, —S(═O)— or —S(═O)2—, and/or in which a —CH-group is replaced by N; and
        wherein each aryl, heteroaryl or heterocyclyl group may be optionally substituted with one or more substituents independently of each other selected from LA;
    
    
    RN independently of each other is selected from the group RN-G1 consisting of H, C1-4-alkyl, C1-4-alkyl-C(═O)—, and C1-4-alkyl-S(═O)2—;
    RNT1 is selected from the group RNT1-G1 consisting of H, C1-6-alkyl, C3-6-cycloalkyl, C1-6-alkyl-C(═O)—, C1-6-alkyl-S(═O)2—, heterocyclyl, aryl and heteroaryl,
    
        wherein each alkyl and cylcoalkyl group may be optionally substituted with one or more substituents independently of each other selected from the group consisting of F, OH, CN, C1-4-alkyl, C1-4-alkyl-O—, RN2N—, C1-4-alkyl-S(═O)2—, C3-6-cycloalkyl, heterocyclyl, phenyl and heteroaryl; and
        wherein heterocyclyl may be optionally substituted with one or more substituents independently of each other selected from F, C1-4-alkyl, RN2N—, OH and C1-4-alkyl-O—; and
        wherein heterocyclyl is a C4-7-cycloalkyl ring in which 1 or 2 —CH2-groups independently of each other are replaced by NRN, O, C(═O), S, S(═O) or S(═O)2; and
        wherein aryl is phenyl or naphthyl; and
        wherein heteroaryl is a 5- or 6-membered aromatic ring which contains 1, 2 or 3 heteroatoms independently of each other selected from N, O and S, wherein the H-atom in one or more NH groups may be optionally replaced by RN; and
        wherein aryl and heteroaryl may be optionally substituted with one or more substituents LA; and
    
    
    RNT2 is selected from the group RNT2-G1 consisting of H and C1-6-alkyl; or
    RNT1 and RNT2 are linked to form one group selected from the group RNT1RNT2-G1 consisting of a C3-5-alkylene group, wherein 1 or 2 —CH2-groups independently of each other are replaced by NRN, O, C(═O), S, S(═O) or S(═O)2; and which may be optionally substituted with one or more substituents independently of each other selected from F, C1-4-alkyl, (RN)2N—, OH and C1-4-alkyl-O—;
    LA is selected from the group LA-G1 consisting of F, Cl, Br, CN, OH, NO2, C1-4-alkyl, C1-4-alkyl-O—, (RN)2N—C(═O)—, (RN)2N—, and C1-4-alkyl-S(═O)2—, wherein each alkyl group may be optionally substituted with one or more substituents independently of each other selected from F, Cl, CN, OH and C1-3-alkyl-O—; and
    RC is selected from the group RC-G1 consisting of F, Cl, Br, I, CN, OH, NO2, C1-6-alkyl, C2-6-alkenyl, C2-6-alkynyl, C3-6-cycloalkyl, C1-6-alkyl-O—, C3-6-cycloalkyl-O—, C1-6-alkyl-S—, HO—C(═O)—, C1-6-alkyl-O—C(═O)—, C1-4-alkyl-C(═O)—, C3-6-cycloalkyl-C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—(RN)N—, heterocyclyl, heterocyclyl-O—, aryl, aryl-O—, heteroaryl and heteroaryl-O—,
    
        wherein each alkyl, alkenyl, alkynyl, and cycloalkyl group may be optionally substituted with one or more substituents independently of each other selected from F, Cl, CN, OH, C1-3-alkyl, C3-6-cycloalkyl, C1-3-alkyl-O—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, aryl, heteroaryl, and heterocyclyl, and
        wherein aryl denotes phenyl or naphthyl, and
        wherein heteroaryl is a 5- or 6-membered aromatic ring which contains 1, 2, 3, or 4 heteroatoms independently of each other selected from N, O and S, wherein the H-atom in one or more NH groups may be optionally replaced by RN; and
        wherein heterocyclyl is a 4- to 7-membered unsaturated or saturated carbocyclic ring in which 1 or 2-CH2-groups independently of each other are replaced by NRN, O, —C(═O)—, S, —S(═O)— or —S(═O)2—, and/or in which a —CH-group is replaced by N; and
        wherein each aryl, heteroaryl or heterocyclyl group may be optionally substituted with one or more substituents independently of each other selected from LA;

including any tautomers and stereoisomers thereof,

or a salt thereof

or a solvate or hydrate thereof,

with the proviso that the following compounds I-a, I-b, and I-c are excluded:

    
    






In a further aspect the present invention relates to processes for preparing a compound of general formula I and to new intermediate compounds in these processes.
A further aspect of the invention relates to a salt of the compounds of general formula I according to this invention, in particular to a pharmaceutically acceptable salt thereof, except for the salts of the compounds I-a, I-b, and I-c.
In a further aspect this invention relates to a pharmaceutical composition, comprising one or more compounds of general formula I including the compounds I-a, I-b, and I-c or one or more pharmaceutically acceptable salts thereof according to the invention, optionally together with one or more inert carriers and/or diluents.
In a further aspect this invention relates to a method for treating diseases or conditions which are mediated by activating the G-protein-coupled receptor GPR119 in a patient in need thereof characterized in that a compound of general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof is administered to the patient.
According to another aspect of the invention, there is provided a method for treating a metabolic disease or disorder in a patient in need thereof characterized in that a compound of general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof is administered to the patient.
According to another aspect of the invention, there is provided the use of a compound of the general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for a therapeutic method as described hereinbefore and hereinafter.
According to another aspect of the invention, there is provided a compound of the general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof for use in a therapeutic method as described hereinbefore and hereinafter.
In a further aspect this invention relates to a method for treating a disease or condition mediated by the activation of the G-protein-coupled receptor GPR119 in a patient that includes the step of administering to the patient in need of such treatment a therapeutically effective amount of a compound of the general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof in combination with a therapeutically effective amount of one or more additional therapeutic agents.
In a further aspect this invention relates to a use of a compound of the general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof in combination with one or more additional therapeutic agents for the treatment of diseases or conditions which are mediated by the activation of the G-protein-coupled receptor GPR119.
In a further aspect this invention relates to a pharmaceutical composition which comprises a compound according to general formula I including the compounds I-a, I-b, and I-c or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents, optionally together with one or more inert carriers and/or diluents.
Other aspects of the invention become apparent to those skilled in the art from the specification and the experimental part as described hereinbefore and hereinafter.


DETAILED DESCRIPTION
Unless otherwise stated, the groups, residues, and substituents, particularly A, R1, R2, T, RN, RNT1, RNT2, LA, and RC are defined as above and hereinafter. If residues, substituents, or groups occur several times in a compound, as for example RN and LA, they may have the same or different meanings. Some preferred meanings of individual groups and substituents of the compounds according to the invention will be given hereinafter. Any and each of these definitions may be combined with each other.
R1:
R1-G1:
The group R1 is preferably selected from the group R1-G1 as defined hereinbefore and hereinafter.
R1-G2:
According to one embodiment the group R1 is selected from the group R1-G2 consisting of:
a) a group selected from:




wherein each group may be optionally substituted with one substituent LA and one substituent Rc;

b) a group selected from —C(═O)—O—(C1-4-alkyl)





and

    
    
        wherein each alkyl residue may be optionally substituted with one or more fluorine atoms; and

c) a group selected from:

    
    






R1-G2a:

According to one embodiment the group R1 is selected from the group R1-G2a consisting of




wherein each group may be optionally substituted with one substituent LA and one substituent RC.

R1-G3:

In another embodiment the group R1 is selected from the group R1-G3 consisting of:
a) a group selected from:




wherein each ring may be optionally substituted with one substituent RC;

b) a group selected from:





wherein each alkyl residue may be optionally substituted with one or more fluorine atoms; and

c) a group selected from:





R1-G3a:

In another embodiment the group R1 is selected from the group R1-G3a consisting of




wherein each ring may be optionally substituted with one substituent RC. Preferably, Rc is selected from the group consisting of Cl, CF3 and C1-3-alkyl.

R1-G3b:

In another embodiment the group R1 is selected from the group R1-G3b consisting of




R1-G3c:

In another embodiment the group R1 is selected from the group R1-G3c consisting of




R1-G4:

In another embodiment the group R1 is selected from the group R1-G4 consisting of




R2:

R2-G1:

The group R2 is preferably selected from the group R2-G1 as defined hereinbefore and hereinafter.
R2-G2:
According to another embodiment the group R2 is selected from the group R2-G2 consisting of H and CH3.
R2-G3:
According to another embodiment the group R2 is selected from the group R2-G3 consisting of H.
R2-G4:
According to another embodiment the group R2 is selected from the group R2-G4 consisting of H3C—.
A:
A-G1:
The group A is preferably selected from the group A-G1 as defined hereinbefore and hereinafter.
A-G2:
In one embodiment the group A is selected from the group A-G2 consisting of

    
    
        a) phenyl, oxazolyl, pyridyl, pyrazinyl, pyridazinyl, and pyrimidinyl, wherein each ring may be substituted with a group T and is optionally substituted with one or two groups independently of each other selected from LA; and
        b) 1,2,3,6-tetrahydropyridin-4-yl and piperazin-1-yl, each of which is substituted at the N with a C1-3-alkyl-S(═O)2— group.

A-G3:

    
    


In another embodiment the group A is selected from the group A-G3 consisting of




    
    
        which is substituted with a group T and may be additionally substituted with one or two groups independently selected from F and H3C—; and
    
    






A-G4:

In another embodiment the group A is selected from the group A-G4 consisting of




    
    
        which may be additionally substituted with one or two F atoms, and
        wherein the group T is defined as hereinbefore and hereinafter; and
    
    






A-G4a:

In another embodiment the group A is selected from the group A-G4a consisting of




which may be additionally substituted with one F atom, and

wherein the group T is defined as hereinbefore and hereinafter.

A-G4b:

In another embodiment the group A is selected from the group A-G4b consisting of




A-G5:

In another embodiment the group A is selected from the group A-G5 consisting of:




A-G6:

In another embodiment the group A is selected from the group A-G6 consisting of




T

T-G1:

The group T is preferably selected from the group T-G1 as defined hereinbefore and hereinafter.
T-G2:
According to one embodiment the group T is selected from the group T-G2 consisting of F, Cl, Br, CN, OH, NO2, C1-4-alkyl, C1-4-alkyl-O—, C1-4-alkyl-O—C(═O)—, C1-4-alkyl-C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, C1-4-alkyl-S(═O)2—C1-4-alkyl-, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, C1-4-alkyl-S(═O)2—(RN)N—, RNT1RNT2N—, RNT1RNT2N—C(═O)—C1-4-alkyl-,

    
    
        wherein each alkyl-group may be optionally substituted with one or more substituents independently of each other selected from F, Cl, CN, OH, aryl, heteroaryl, and heterocyclyl,
        wherein aryl denotes phenyl or naphthyl,
        wherein heteroaryl is selected from the group consisting of pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, imidazolyl, [1,2,4]triazolyl and tetrazolyl;
        wherein heterocyclyl is selected from the group consisting of azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl and morpholinyl, in each of which a group may be optionally replaced by a group selected from —C(═O)— and —S(═O)2—, wherein heterocyclyl group may be optionally substituted with one or more substituents independently of each other selected from C1-3-alkyl; and
        wherein each phenyl and heteroaryl may be optionally substituted with one or more substituents LA, wherein the substituents may be identical or different.

T-G3:

    
    


According to another embodiment the group T is selected from the group T-G3 consisting of —CN, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)2—(RN)N—, and RNT1RNT2N—.
T-G4:
According to another embodiment the group T is selected from the group T-G4 consisting of NC—, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2, (C1-4-alkyl)NH—C(═O)—, and C1-4-alkyl-S(═O)2—NH—.
T-G5:
According to another embodiment the group T is selected from the group T-G5 consisting of NC—, H2N—C(═O)—, CH3—CH2—NH—C(═O)—, CH3—S(═O)2—CH2—, and CH3—S(═O)2—.
RN 
RN-G1:
The group RN is preferably selected from the group RN-G1 as defined hereinbefore and hereinafter.
RN-G2:
In another embodiment the group RN is selected from the group RN-G2 consisting of H, methyl, ethyl, isopropyl, methylcarbonyl, and methylsulfonyl.
RN-G3:
In another embodiment the group RN is selected from the group RN-G3 consisting of H, methyl, methylcarbonyl, and methylsulfonyl.
RN-G4:
In another embodiment the group RN is selected from the group RN-G4 consisting of H and methyl.
RNT1 
RNT1-G1:
RNT1 is preferably selected from the group RNT1-G1 as defined hereinbefore and hereinafter.
RNT1-G2:
In another embodiment RNT1 is selected from the group RNT1-G2 consisting of H, C1-4-alkyl, C3-6-cycloalkyl and tetrahydropyranyl,

    
    
        wherein each alkyl and cycloalkyl group may be optionally substituted with one substituent selected from the group consisting of F, CH3, OH, C1-3-alkyl-O—, (RN)2N—, CN, tetrahydrofuranyl, 1,4-dioxinyl, oxazolyl, and pyridyl.

RNT1-G3:

    
    


In another embodiment RNT1 is selected from the group RNT1-G3 consisting of H, C1-4-alkyl and C3-6-cycloalkyl,

    
    
        wherein each alkyl and cycloalkyl group may be optionally substituted with one substituent selected from F, CH3, OH, and C1-3-alkyl-O—.

RNT2-G4:

    
    


In another embodiment RNT1 is selected from the group RNT1-G4 consisting of H and C1-4-alkyl.
RNT2 
RNT2-G1:
RNT2 is preferably selected from the group RNT2-G1 as defined hereinbefore and hereinafter.
RNT2-G2:
In another embodiment RNT2 is selected from the group RNT2-G2 consisting of H and C1-3-alkyl.
RNT2-G3:
In another embodiment RNT2 is selected from the group RNT2-G3 consisting of H and methyl.
RNT2-G4:
In another embodiment RNT2 is selected from the group RNT2-G4 consisting of H.
RNT1RNT2 
RNT1RNT2-G1:
According to one embodiment the groups RNT1 and RNT2 are linked and form a group which is selected from the group RNT1RNT2-G1 as defined hereinbefore and hereinafter.
RNT1RNT2-G2:
According to another embodiment the groups RNT1 and RNT2 are linked and together with the N-atom to which they are attached form a group which is selected from the group RNT1RNT2-G2 consisting of:
azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, piperazinyl, piperazin-2-onyl, N—(C1-3-alkyl)-piperazinyl, N—(C1-3-alkyl)-piperazin-2-onyl, and N—(C1-3-alkyl-C(═O))-piperazinyl,


    
    
        which may be optionally substituted with one or more substituents independently of each other selected from the group consisting of F, HO, C1-3-alkyl, C1-3-alkyl-O—, and (RN)2N—.

RNT1RNT2-G3:

    
    


According to another embodiment the groups RNT1 and RNT2 are linked and together with the N-atom to which they are attached form a group which is selected from the group RNT1RNT2-G3 consisting of:
azetidinyl, pyrrolidinyl, piperidinyl, and morpholinyl, each of which may be optionally substituted with one or two substituents independently of each other selected from the group consisting of F, OH, CH3 and CH3—O—.
LA:
LA-G1:
The group LA is preferably selected from the group LA-G1 as defined hereinbefore and hereinafter.
LA-G2:
In another embodiment the group LA is selected from the group LA-G2 consisting of F, Cl, Br, CN, OH, C1-3-alkyl-, C1-3-alkyl-O—, H2N—, C1-3-alkyl-NH— and (C1-3-alkyl)2N—, wherein the C1-3-alkyl- and C1-3-alkyl-O— group may be optionally substituted with one or more F-atoms.
LA-G3:
In another embodiment the group LA is selected from the group LA-G3 consisting of F, Cl, CH3, and CF3.
LA-G4:
In another embodiment the group LA is selected from the group LA-G4 consisting of F.
RC:
RC-G1:
The group RC is preferably selected from the group RC-G1 as defined hereinbefore and hereinafter.
RC-G2:
According to one embodiment the group RC is selected from the group RC-G2 consisting of F, Cl, Br, I, CN, OH, C1-6-alkyl, C2-6-alkenyl, C2-6-alkynyl, C3-6-cycloalkyl, C1-6-alkyl-O—, C3-6-cycloalkyl-O—, C1-6-alkyl-S—, C1-6-alkyl-O—C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)—C1-4-alkyl-, C1-4-alkyl-S(═O)2—, C1-4-alkyl-S(═O)2—C1-4-alkyl-, RNT1RNT2N—, RNT1RNT2N—C1-3-alkyl-, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—S(═O)2—C1-4-alkyl-, RNT1RNT2N—C(═O)—C1-4-alkyl-, heterocyclyl, heterocyclyl-O—, and phenyl,

    
    
        wherein each alkyl, alkenyl, alkynyl, and cycloalkyl group may be optionally substituted with one or more fluorine atoms and a substituent selected from Cl, CN, OH, C1-3-alkyl, C3-6-cycloalkyl, C1-3-alkyl-O—, and heterocyclyl;
        wherein heterocyclyl is selected from the group consisting of pyrrolidin-2-onyl, piperidin-2-onyl, piperazin-2-onyl, morpholinyl, morpholin-3-onyl, oxetanyl, tetrahydrofuranyl, and tetrahydropyranyl, each of which may be optionally substituted with one or two H3C— groups; and
        wherein phenyl may be optionally substituted independently of each other with one or more substituents LA.

RC-G3:

    
    


According to another embodiment the group RC is selected from the group RC-G3 consisting of F, Cl, Br, CN, C1-4-alkyl, C3-6-cycloalkyl, C1-4-alkyl-O—, C3-6-cycloalkyl-O—, heterocyclyl, and heterocyclyl-O—,

    
    
        wherein each alkyl and cycloalkyl group may be optionally substituted with one or more fluorine atoms and a substituent selected from H3C— and H3C—O—, and
        wherein heterocyclyl is selected from the group consisting of oxetanyl, tetrahydrofuranyl, and tetrahydropyranyl, each of which may be optionally substituted with one or two H3C— groups.

RC-G4:

    
    


According to another embodiment the group RC is selected from the group RC-G4 consisting of Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, and C1-3-alkyl-O—.
RC-G5:
According to another embodiment the group RC is selected from the group RC-G5 consisting of Cl, F3C— and C1-3-alkyl.
A preferred embodiment of compounds of the formula I are described by formula (I.1), wherein any tautomers and stereoisomers, solvates, hydrates and salts thereof, in particular the pharmaceutically acceptable salts thereof, are encompassed.




Examples of preferred subgeneric embodiments according to the present invention are set forth in the following table, wherein each substituent group of each embodiment is defined according to the definitions set forth hereinbefore and wherein all other substituents of the formula I are defined according to the definitions as set forth hereinbefore:

















Embodiment
R1-
A-
T-
RC-









E-1
R1-G1
A-G1
T-G1
RC-G1



E-2
R1-G2
A-G2
T-G3
RC-G3



E-3
R1-G2
A-G2
T-G3
RC-G4



E-4
R1-G2
A-G2
T-G3
RC-G5



E-5
R1-G2
A-G2
T-G4
RC-G3



E-6
R1-G2
A-G2
T-G4
RC-G4



E-7
R1-G2
A-G2
T-G4
RC-G5



E-8
R1-G2
A-G2
T-G5
RC-G3



E-9
R1-G2
A-G2
T-G5
RC-G4



E-10
R1-G2
A-G2
T-G5
RC-G5



E-11
R1-G2
A-G3
T-G3
RC-G3



E-12
R1-G2
A-G3
T-G3
RC-G4



E-13
R1-G2
A-G3
T-G3
RC-G5



E-14
R1-G2
A-G3
T-G4
RC-G3



E-15
R1-G2
A-G3
T-G4
RC-G4



E-16
R1-G2
A-G3
T-G4
RC-G5



E-17
R1-G2
A-G3
T-G5
RC-G3



E-18
R1-G2
A-G3
T-G5
RC-G4



E-19
R1-G2
A-G3
T-G5
RC-G5



E-20
R1-G2
A-G4
T-G3
RC-G3



E-21
R1-G2
A-G4
T-G3
RC-G4



E-22
R1-G2
A-G4
T-G3
RC-G5



E-23
R1-G2
A-G4
T-G4
RC-G3



E-24
R1-G2
A-G4
T-G4
RC-G4



E-25
R1-G2
A-G4
T-G4
RC-G5



E-26
R1-G2
A-G4
T-G5
RC-G3



E-27
R1-G2
A-G4
T-G5
RC-G4



E-28
R1-G2
A-G4
T-G5
RC-G5



E-29
R1-G2
A-G4a
T-G3
RC-G3



E-30
R1-G2
A-G4a
T-G3
RC-G4



E-31
R1-G2
A-G4a
T-G3
RC-G5



E-32
R1-G2
A-G4a
T-G4
RC-G3



E-33
R1-G2
A-G4a
T-G4
RC-G4



E-34
R1-G2
A-G4a
T-G4
RC-G5



E-35
R1-G2
A-G4a
T-G5
RC-G3



E-36
R1-G2
A-G4a
T-G5
RC-G4



E-37
R1-G2
A-G4a
T-G5
RC-G5



E-38
R1-G2
A-G4b
—
RC-G3



E-39
R1-G2
A-G4b
—
RC-G4



E-40
R1-G2
A-G4b
—
RC-G5



E-41
R1-G2
A-G5
—
RC-G3



E-42
R1-G2
A-G5
—
RC-G4



E-43
R1-G2
A-G5
—
RC-G5



E-44
R1-G3
A-G2
T-G3
RC-G3



E-45
R1-G3
A-G2
T-G3
RC-G4



E-46
R1-G3
A-G2
T-G3
RC-G5



E-47
R1-G3
A-G2
T-G4
RC-G3



E-48
R1-G3
A-G2
T-G4
RC-G4



E-49
R1-G3
A-G2
T-G4
RC-G5



E-50
R1-G3
A-G2
T-G5
RC-G3



E-51
R1-G3
A-G2
T-G5
RC-G4



E-52
R1-G3
A-G2
T-G5
RC-G5



E-53
R1-G3
A-G3
T-G3
RC-G3



E-54
R1-G3
A-G3
T-G3
RC-G4



E-55
R1-G3
A-G3
T-G3
RC-G5



E-56
R1-G3
A-G3
T-G4
RC-G3



E-57
R1-G3
A-G3
T-G4
RC-G4



E-58
R1-G3
A-G3
T-G4
RC-G5



E-59
R1-G3
A-G3
T-G5
RC-G3



E-60
R1-G3
A-G3
T-G5
RC-G4



E-61
R1-G3
A-G3
T-G5
RC-G5



E-62
R1-G3
A-G4
T-G3
RC-G3



E-63
R1-G3
A-G4
T-G3
RC-G4



E-64
R1-G3
A-G4
T-G3
RC-G5



E-65
R1-G3
A-G4
T-G4
RC-G3



E-66
R1-G3
A-G4
T-G4
RC-G4



E-67
R1-G3
A-G4
T-G4
RC-G5



E-68
R1-G3
A-G4
T-G5
RC-G3



E-69
R1-G3
A-G4
T-G5
RC-G4



E-70
R1-G3
A-G4
T-G5
RC-G5



E-71
R1-G3
A-G4a
T-G3
RC-G3



E-72
R1-G3
A-G4a
T-G3
RC-G4



E-73
R1-G3
A-G4a
T-G3
RC-G5



E-74
R1-G3
A-G4a
T-G4
RC-G3



E-75
R1-G3
A-G4a
T-G4
RC-G4



E-76
R1-G3
A-G4a
T-G4
RC-G5



E-77
R1-G3
A-G4a
T-G5
RC-G3



E-78
R1-G3
A-G4a
T-G5
RC-G4



E-79
R1-G3
A-G4a
T-G5
RC-G5



E-80
R1-G3
A-G4b
—
RC-G3



E-81
R1-G3
A-G4b
—
RC-G4



E-82
R1-G3
A-G4b
—
RC-G5



E-83
R1-G3
A-G5
—
RC-G3



E-84
R1-G3
A-G5
—
RC-G4



E-85
R1-G3
A-G5
—
RC-G5



E-86
R1-G4
A-G2
T-G3
—



E-87
R1-G4
A-G2
T-G4
—



E-88
R1-G4
A-G2
T-G5
—



E-89
R1-G4
A-G3
T-G3
—



E-90
R1-G4
A-G3
T-G4
—



E-91
R1-G4
A-G3
T-G5
—



E-92
R1-G4
A-G4
T-G3
—



E-93
R1-G4
A-G4
T-G4
—



E-94
R1-G4
A-G4
T-G5
—



E-95
R1-G4
A-G4a
T-G3
—



E-96
R1-G4
A-G4a
T-G4
—



E-97
R1-G4
A-G4a
T-G5
—



E-98
R1-G4
A-G4b
—
—



E-99
R1-G4
A-G5
—
—



E-100
R1-G3a
A-G4
T-G3
RC-G3



E-101
R1-G3a
A-G4
T-G3
RC-G4



E-102
R1-G3a
A-G4
T-G3
RC-G5



E-103
R1-G3a
A-G4
T-G4
RC-G3



E-104
R1-G3a
A-G4
T-G4
RC-G4



E-105
R1-G3a
A-G4
T-G4
RC-G5



E-106
R1-G3a
A-G4
T-G5
RC-G3



E-107
R1-G3a
A-G4
T-G5
RC-G4



E-108
R1-G3a
A-G4
T-G5
RC-G5



E-109
R1-G3a
A-G4a
T-G3
RC-G3



E-110
R1-G3a
A-G4a
T-G3
RC-G4



E-111
R1-G3a
A-G4a
T-G3
RC-G5



E-112
R1-G3a
A-G4a
T-G4
RC-G3



E-113
R1-G3a
A-G4a
T-G4
RC-G4



E-114
R1-G3a
A-G4a
T-G4
RC-G5



E-115
R1-G3a
A-G4a
T-G5
RC-G3



E-116
R1-G3a
A-G4a
T-G5
RC-G4



E-117
R1-G3a
A-G4a
T-G5
RC-G5



E-118
R1-G3a
A-G4b
—
RC-G3



E-119
R1-G3a
A-G4b
—
RC-G4



E-120
R1-G3a
A-G4b
—
RC-G5



E-121
R1-G3b
A-G4
T-G3
—



E-122
R1-G3b
A-G4
T-G4
—



E-123
R1-G3b
A-G4
T-G5
—



E-124
R1-G3b
A-G4a
T-G3
—



E-125
R1-G3b
A-G4a
T-G4
—



E-126
R1-G3b
A-G4a
T-G5
—



E-127
R1-G3b
A-G4b
—
—



E-128
R1-G2
A-G6
—
RC-G3



E-129
R1-G2
A-G6
—
RC-G4



E-130
R1-G2
A-G6
—
RC-G5



E-131
R1-G3
A-G6
—
RC-G3



E-132
R1-G3
A-G6
—
RC-G4



E-133
R1-G3
A-G6
—
RC-G5



E-134
R1-G4
A-G6
—
—










Preferred are those compounds of formula I, wherein
R1 is selected from the group consisting of:




    
    
        wherein each ring may be optionally substituted with one substituent RC; and wherein RC is selected from the group consisting of Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, and C1-3-alkyl-O—;
    
    






    
    
        wherein each alkyl residue may be optionally substituted with one or more fluorine atoms; and
    
    






R2 is H; and
A is selected from the group consisting of:




    
    
        wherein T is selected from the group consisting of NC—, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2, RNT1RNT2N—C(═O)—, and C1-4-alkyl-S(═O)2—NH—; and which may be additionally substituted with one or two F atoms; and
    
    






and the pharmaceutically acceptable salts thereof.

More preferred are those compounds of formula I, wherein
R1 is selected from the group consisting of:




    
    
        wherein each ring may be optionally substituted with one substituent RC; and
        wherein RC is selected from the group consisting of Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, and C1-3-alkyl-O—;
    
    






    
    
        wherein each alkyl residue may be optionally substituted with one or more fluorine atoms; and
    
    






and

R2 is H; and
A is selected from the group consisting of:




and the pharmaceutically acceptable salts thereof.

Particularly preferred compounds, including their tautomers and stereoisomers, the salts thereof, or any solvates or hydrates thereof, are described in the experimental section hereinafter.
The compounds according to the invention and their intermediates may be obtained using methods of synthesis which are known to those skilled in the art and described in the literature of organic synthesis. Preferably the compounds are obtained analogously to the methods of preparation explained more fully hereinafter, in particular as described in the experimental section. In some cases the sequence adopted in carrying out the reaction schemes may be varied. Variants of these reactions that are known to a person skilled in the art but are not described in detail here may also be used. The general processes for preparing the compounds according to the invention will become apparent to a person skilled in the art on studying the schemes that follow. Starting compounds are commercially available or may be prepared by methods that are described in the literature or herein, or may be prepared in an analogous or similar manner. Before the reaction is carried out any corresponding functional groups in the compounds may be protected using conventional protecting groups. These protecting groups may be cleaved again at a suitable stage within the reaction sequence using methods familiar to a person skilled in the art.
Compounds of the invention I may be obtained from compound 1, bearing two replaceable halogen or pseudo-halogen groups, as described in Scheme 1; R1, R2, and A have the meanings as defined hereinbefore and hereinafter. Depending on the reactivity of the two carbon atoms bearing the halogen or pseudo-halogen groups, the two coupling partners, 3 and 5, are introduced following the sequence depicted on the top or bottom of the scheme. Both residues are preferably attached via a transition metal catalyzed reaction, preferably mediated by a palladium, nickel, copper, or iron species. The active catalyst may be derived from an elemental form of the transition metal, such as palladium on carbon or nanoparticles of iron or palladium, or a salt of the transition metal, such as fluoride, chloride, bromide, acetate, triflate, or trifluoroacetate, which are preferably combined with ligands, such as phosphines, e.g. tri-tert-butylphosphine, tricyclohexylphosphine, optionally substituted biphenyl-dicyclohexyl-phosphines, optionally substituted biphenyl-di-tert-butyl-phosphines, 1,1′-bis(diphenylphosphino)-ferrocene, triphenylphosphine, tritolylphosphine, or trifurylphosphine, phosphites, 1,3-disubstituted imidazole carbenes, 1,3-disubstituted imidazolidine carbenes, dibenzylideneacetone, allyl, or nitriles. A-M is preferably a boronic acid, trifluoroborate, boronic ester, zinc halide, or magnesium halide of A and alkyne 3 is preferably used as is or zinc acetylide. Depending on the nucleophiles the reactions are preferably conducted in benzene, toluene, ether, tetrahydrofuran, 1,2-dimethoxyethane, 1,4-dioxane, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidinone, alcohol, water, or mixtures thereof, at −10 to 180° C. Additives such as halide salts, e.g. lithium chloride, potassium fluoride, tetrabutylammonium fluoride, hydroxide sources, such as potassium hydroxide, potassium carbonate, amines, such as triethylamine, diisopropylamine, and ethyldiisopropylamine, silver salts, such as silver oxide or triflate, and/or copper salts, such as copper iodide or chloride or copper thiophene-2-carboxylate, may be beneficial or even essential for the reaction to proceed. The conditions for the coupling of alkyne 3 with one of the electrophiles, 1 or 4, may bring about the subsequent cyclization as well and thus provide the benzofuran. For instance, with Pd(PPh3)2Cl2, CuI, and triethylamine in N,N-dimethylformamide at 20 to 140° C. the benzofuran may be obtained directly. If the intermediate alkyne 6 is obtained the benzofuran may be formed in a separate step using, for example, n-Bu4NF in tetrahydrofuran at 50 to 70° C., NaOH in aqueous solution at elevated temperature, CuI or CuCN, optionally in the presence of NEt3, in N,N-dimethylformamide at elevated temperature, AuCl(PPh3) and AgOSO2CF3 in CH2Cl2 or tetrahydrofuran, AgOSO2CF3, optionally in the presence of trifluoroacetic acid, in CH2Cl2, Pd, e.g. PdCl2, or other transition metals such as Rh. The reactivities of the reaction partners (reacting carbons) described may be reversed, i.e. compounds 1, 2, and 4 are the nucleophile bearing M and compounds 3 and 5 are the electrophile bearing Hal1 or Hal2, providing the same products under the same or similar conditions.




Residue R1 is attached to the N atom of the piperidine of the compounds of the invention via different routes depending on the nature of residue R1 (Scheme 2); R1, R2, and A have the meanings as defined hereinbefore and hereinafter. A heteroaryl group may be attached via a nucleophilic substitution of a leaving group on the heteroaromatic, such as F, Cl, Br, SO2C1-4-alkyl, and SO2aryl. The reaction is preferably conducted in the presence of a base, e.g. Na2CO3, K2CO3, CS2CO3, triethylamine, ethyldiisopropylamine, and 1,8-diazabicylo[5.4.0]undec-7-ene, in toluene, tetrahydrofuran, 1,4-dioxane, 1,2-dimethoxyethane, acetonitrile, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidinone, water, alcohol, dimethyl sulfoxide, or mixtures thereof, at 20 to 220° C. by conventional or microwave heating. In certain cases the use of transition metal catalysts may be beneficial or even essential. The leaving group Y in compound 8 is then preferably Cl, Br, I, and OSO2CF3. Some heteroaromatic residues R1 such as [1,2,4]oxadiazoles may also be assembled from the corresponding cyanamide of compound 7 and N-hydroxyamidine.
A carbamate group may be formed from compound 7 and an oxycarbonyl electrophile (R1═RaO—C(═O)), such as RaO—C(═O)—Cl or RaO—C(═O)—O—C(═O)—ORa). The reaction is usually conducted in the presence of a base, e.g. K2CO3, pyridine, 4-dimethylaminopyridine, triethylamine, or ethyldiisopropylamine, in a solvent such as toluene, dichloromethane, ether, tetrahydrofuran, 1,4-dioxane, 1,2-dimethoxyethane, acetonitrile, or mixtures thereof, at −10 to 120° C.
A methylene group may be attached to the piperidine N by using an R1 group bearing a leaving group at the CH2 unit to be attached, such as Cl, Br, I, OSO2C1-4-alkyl, OSO2aryl, or OSO2CF3. The substitution is commonly done in the presence of a base, e.g. Na2CO3, K2CO3, Cs2CO3, pyridine, triethylamine, ethyldiisopropylamine, and 1,8-diazabicylo[5.4.0]undec-7-ene, in a solvent such as toluene, tetrahydrofuran, 1,4-dioxane, 1,2-dimethoxyethane, acetonitrile, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidinone, water, methanol, ethanol, isopropanol, dimethyl sulfoxide, or mixtures thereof, at 20 to 220° C. by conventional or microwave heating.
Alternatively, the CH2 moiety of residue R1 is formed during the attachment reaction by reductive alkylation employing R1 as aldehyde and a reducing agent, such as NaH3BCN or NaHB(O2CCH3)3. Using a carboxylic acid derivative of R1 also allows the attachment of R1 to intermediate 7 via a CH2 unit by a two step procedure, amide coupling followed by reduction of the amide carbonyl group.
The proceedings described for attaching group R1 to the N of the piperidine moiety may be employed for any suited precursor molecule of compound 7, too, such as compound 3 in Scheme 1.




The synthetic routes presented may rely on the use of protecting groups. For example, potentially reactive groups present, such as hydroxy, carbonyl, carboxy, amino, alkylamino, or imino, may be protected during the reaction by conventional protecting groups which are cleaved again after the reaction. Suitable protecting groups for the respective functionalities and their removal are well known to a person skilled in the art and are described in the literature of organic synthesis.
The compounds of general formula I may be resolved into their enantiomers and/or diastereomers as mentioned before. Thus, for example, cis/trans mixtures may be resolved into their cis and trans isomers and racemic compounds may be separated into their enantiomers.
The cis/trans mixtures may be resolved, for example, by chromatography into the cis and trans isomers thereof. The compounds of general formula I which occur as racemates may be separated by methods known per se into their optical antipodes and diastereomeric mixtures of compounds of general formula I may be resolved into their diastereomers by taking advantage of their different physico-chemical properties using methods known per se, e.g. chromatography and/or fractional crystallization; if the compounds obtained thereafter are racemates, they may be resolved into the enantiomers as mentioned above.
The racemates are preferably resolved by column chromatography on chiral phases or by crystallization from an optically active solvent or by reacting with an optically active substance which forms salts or derivatives such as esters or amides with the racemic compound. Salts may be formed with enantiomerically pure acids for basic compounds and with enantiomerically pure bases for acidic compounds. Diastereomeric derivatives are formed with enantiomerically pure auxiliary compounds, e.g. acids, their activated derivatives, or alcohols. Separation of the diastereomeric mixture of salts or derivatives thus obtained may be achieved by taking advantage of their different physico-chemical properties, e.g. differences in solubility; the free antipodes may be released from the pure diastereomeric salts or derivatives by the action of suitable agents. Optically active acids commonly used for such a purpose as well as optically active alcohols applicable as auxiliary residues are known to those skilled in the art.
As mentioned above, the compounds of formula I may be converted into salts, particularly for pharmaceutical use into the pharmaceutically acceptable salts. As used herein, “pharmaceutically acceptable salts” refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof.
The compounds according to the invention are advantageously also obtainable using the methods described in the examples that follow, which may also be combined for this purpose with methods known to a person skilled in the art from the literature.
TERMS AND DEFINITIONS
Terms not specifically defined herein have the meaning that a person skilled in the art would attribute to in light of the disclosure and the context. As used in the specification, however, unless specified to the contrary, the following terms have the meaning indicated and the following conventions are adhered to.
The terms “compound(s) according to this invention”, “compound(s) of formula (I)”, “compound(s) of the invention” and the like denote the compounds of the formula (I) according to the present invention including their tautomers, stereoisomers and mixtures thereof and the salts thereof, in particular the pharmaceutically acceptable salts thereof, and the solvates and hydrates of such compounds, including the solvates and hydrates of such tautomers, stereoisomers and salts thereof.
The terms “treatment” and “treating” embrace both preventative, i.e. prophylactic, or therapeutic, i.e. curative and/or palliative, treatment. Thus the terms “treatment” and “treating” comprise therapeutic treatment of patients having already developed said condition, in particular in manifest form. Therapeutic treatment may be symptomatic treatment in order to relieve the symptoms of the specific indication or causal treatment in order to reverse or partially reverse the conditions of the indication or to stop or slow down progression of the disease. Thus the compositions and methods of the present invention may be used for instance as therapeutic treatment over a period of time as well as for chronic therapy. In addition the terms “treatment” and “treating” comprise prophylactic treatment, i.e. a treatment of patients at risk to develop a condition mentioned hereinbefore, thus reducing said risk.
When this invention refers to patients requiring treatment, it relates primarily to treatment in mammals, in particular humans.
The term “therapeutically effective amount” means an amount of a compound of the present invention that (i) treats or prevents the particular disease or condition, (ii) attenuates, ameliorates, or eliminates one or more symptoms of the particular disease or condition, or (iii) prevents or delays the onset of one or more symptoms of the particular disease or condition described herein.
The terms “modulated” or “modulating”, or “modulate(s)”, as used herein, unless otherwise indicated, refer to the activation of the G-protein-coupled receptor GPR119 with one or more compounds of the present invention.
The terms “mediated” or “mediating” or “mediate”, as used herein, unless otherwise indicated, refer to the (i) treatment, including prevention of the particular disease or condition, (ii) attenuation, amelioration, or elimination of one or more symptoms of the particular disease or condition, or (iii) prevention or delay of the onset of one or more symptoms of the particular disease or condition described herein.
The term “substituted” as used herein, means that any one or more hydrogens on the designated atom, radical or moiety is replaced with a selection from the indicated group, provided that the atom's normal valence is not exceeded, and that the substitution results in an acceptably stable compound.
In the groups, radicals, or moieties defined below, the number of carbon atoms is often specified preceding the group, for example, C1-6-alkyl means an alkyl group or radical having 1 to 6 carbon atoms. In general, for groups comprising two or more subgroups, the last named subgroup is the radical attachment point, for example, the substituent “aryl-C1-3-alkyl-” means an aryl group which is bound to a C1-3-alkyl-group, the latter of which is bound to the core or to the group to which the substituent is attached.
In case a compound of the present invention is depicted in form of a chemical name and as a formula, the formula shall prevail in case of any discrepancy.
An asterisk is may be used in sub-formulas to indicate the bond which is connected to the core molecule as defined.
The numeration of the atoms of a substituent starts with the atom which is closest to the core or to the group to which the substituent is attached.
For example, the term “3-carboxypropyl-group” represents the following substituent:




wherein the carboxy group is attached to the third carbon atom of the propyl group. The terms “1-methylpropyl-”, “2,2-dimethylpropyl-” or “cyclopropylmethyl-” group represent the following groups:





The asterisk may be used in sub-formulas to indicate the bond which is connected to the core molecule as defined.
In a definition of a group the term “wherein each X, Y and Z group is optionally substituted with” and the like denotes that each group X, each group Y and each group Z either each as a separate group or each as part of a composed group may be substituted as defined. For example a definition “Rex denotes H, C1-3-alkyl, C3-6-cycloalkyl, C3-6-cycloalkyl-C1-3-alkyl or C1-3-alkyl-O—, wherein each alkyl group is optionally substituted with one or more Lex.” or the like means that in each of the beforementioned groups which comprise the term alkyl, i.e. in each of the groups C1-3-alkyl, C3-6-cycloalkyl-C1-3-alkyl and C1-3-alkyl-O—, the alkyl moiety may be substituted with Lex as defined.
In the following the term bicyclic includes spirocyclic.
Unless specifically indicated, throughout the specification and the appended claims, a given chemical formula or name shall encompass tautomers and all stereo, optical and geometrical isomers (e.g. enantiomers, diastereomers, E/Z isomers etc. . . . ) and racemates thereof as well as mixtures in different proportions of the separate enantiomers, mixtures of diastereomers, or mixtures of any of the foregoing forms where such isomers and enantiomers exist, as well as salts, including pharmaceutically acceptable salts thereof and solvates thereof such as for instance hydrates including solvates of the free compounds or solvates of a salt of the compound.
The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, and commensurate with a reasonable benefit/risk ratio.
As used herein, the term “pharmaceutically acceptable salts” refers to derivatives of the disclosed compounds wherein the parent compound is modified by making a salt thereof with a suitable organic or inorganic acid or base.
Salts that are for example useful for purifying or isolating the compounds of the present invention are also an embodiment of the invention.
The term halogen generally denotes fluorine, chlorine, bromine and iodine.
The term “C1-n-alkyl”, wherein n is an integer from 1 to n, either alone or in combination with another radical denotes an acyclic, saturated, branched or linear hydrocarbon radical with 1 to n C atoms. For example the term C1-5-alkyl embraces the radicals H3C—, H3C—CH2—, H3C—CH2—CH2—, H3C—CH(CH3)—, H3C—CH2—CH2—CH2—, H3C—CH2—CH(CH3)—, H3C—CH(CH3)—CH2—, H3C—C(CH3)2—, H3C—CH2—CH2—CH2—CH2—, H3C—CH2—CH2—CH(CH3)—, H3C—CH2—CH(CH3)—CH2—, H3C—CH(CH3)—CH2—CH2—, H3C—CH2—C(CH3)2—, H3C—C(CH3)2—CH2—, H3C—CH(CH3)—CH(CH3)— and H3C—CH2—CH(CH2CH3)—.
The term “C1-n-alkylene” wherein n is an integer 1 to n, either alone or in combination with another radical, denotes an acyclic, straight or branched chain divalent alkyl radical containing from 1 to n carbon atoms. For example the term C1-4-alkylene includes —(CH2)—, —(CH2—CH2)—, —(CH(CH3))—, —(CH2—CH2—CH2)—, —(C(CH3)2)—, —(CH(CH2CH3))—, —(CH(CH3)—CH2)—, —(CH2—CH(CH3))—, —(CH2—CH2—CH2—CH2)—, —(CH2—CH2—CH(CH3))—, —(CH(CH3)—CH2—CH2)—, —(CH2—CH(CH3)—CH2)—, —(CH2—C(CH3)2)—, —(C (CH3)2—CH2)—, —(CH(CH3)—CH(CH3))—, —(CH2—CH(CH2CH3))—, —(CH(CH2CH3)—CH2)—, —(CH(CH2CH2CH3))—, —(CHCH(CH3)2)— and —C(CH3)(CH2CH3)—.
The term “C2-n-alkenyl”, is used for a group as defined in the definition for “C1-n-alkyl” with at least two carbon atoms, if at least two of those carbon atoms of said group are bonded to each other by a double bond. For example the term C2-3-alkenyl includes —CH═CH2, —CH═CH—CH3, —CH2—CH═CH2.
The term “C2-n-alkenylene” is used for a group as defined in the definition for “C1-n-alkylene” with at least two carbon atoms, if at least two of those carbon atoms of said group are bonded to each other by a double bond. For example the term C2-3-alkenylene includes —CH═CH—, —CH═CH—CH2—, —CH2—CH═CH—.
The term “C2-n-alkynyl”, is used for a group as defined in the definition for “C1-n-alkyl” with at least two carbon atoms, if at least two of those carbon atoms of said group are bonded to each other by a triple bond. For example the term C2-3-alkynyl includes —C≡CH, —C≡C—CH3, —CH2—C≡CH.
The term “C2-n-alkynylene” is used for a group as defined in the definition for “C1-n-alkylene” with at least two carbon atoms, if at least two of those carbon atoms of said group are bonded to each other by a triple bond. For example the term C2-3-alkynylene includes —C≡C—, —C≡C—CH2—, —CH2—C≡C—.
The term “C3-n-carbocyclyl” as used either alone or in combination with another radical, denotes a monocyclic, bicyclic or tricyclic, saturated or unsaturated hydrocarbon radical with 3 to n C atoms. The hydrocarbon radical is preferably nonaromatic. Preferably the 3 to n C atoms form one or two rings. In case of a bicyclic or tricyclic ring system the rings may be attached to each other via a single bond or may be fused or may form a spirocyclic or bridged ring system. For example the term C3-10-carbocyclyl includes C3-10-cycloalkyl, C3-10-cycloalkenyl, octahydropentalenyl, octahydroindenyl, decahydronaphthyl, indanyl, tetrahydronaphthyl. Most preferably the term C3-n-carbocyclyl denotes C3-n-cycloalkyl, in particular C3-7-cycloalkyl.
The term “C3-n-cycloalkyl”, wherein n is an integer 4 to n, either alone or in combination with another radical denotes a cyclic, saturated, unbranched hydrocarbon radical with 3 to n C atoms. The cyclic group may be mono-, bi-, tri- or spirocyclic, most preferably monocyclic. Examples of such cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclododecyl, bicyclo[3.2.1]octyl, spiro[4.5]decyl, norpinyl, norbonyl, norcaryl, adamantyl, etc.
The term “C3-n-cycloalkenyl”, wherein n is an integer 3 to n, either alone or in combination with another radical, denotes a cyclic, unsaturated but nonaromatic, unbranched hydrocarbon radical with 3 to n C atoms, at least two of which are bonded to each other by a double bond. For example the term C3-7-cycloalkenyl includes cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclopentadienyl, cyclohexenyl, cyclohexadienyl, cycloheptenyl, cycloheptadienyl and cycloheptatrienyl.
The term “aryl” as used herein, either alone or in combination with another radical, denotes a carbocyclic aromatic monocyclic group containing 6 carbon atoms which may be further fused to a second 5- or 6-membered carbocyclic group which may be aromatic, saturated or unsaturated. Aryl includes, but is not limited to, phenyl, indanyl, indenyl, naphthyl, anthracenyl, phenanthrenyl, tetrahydronaphthyl and dihydronaphthyl. More preferably the term “aryl” as used herein, either alone or in combination with another radical, denotes phenyl or naphthyl, most preferably phenyl.
The term “heterocyclyl” means a saturated or unsaturated mono-, bi-, tri- or spirocarbocyclic, preferably mono-, bi- or spirocyclic-ring system containing one or more heteroatoms selected from N, O or S(O)r with r=0, 1 or 2, which in addition may have a carbonyl group. More preferably the term “heterocyclyl” as used herein, either alone or in combination with another radical, means a saturated or unsaturated, even more preferably a saturated mono-, bi- or spirocyclic-ring system containing 1, 2, 3 or 4 heteroatoms selected from N, O or S(O)r with r=0, 1 or 2 which in addition may have a carbonyl group. The term “heterocyclyl” is intended to include all the possible isomeric forms. Examples of such groups include aziridinyl, oxiranyl, azetidinyl, oxetanyl, pyrrolidinyl, tetrahydrofuranyl, piperidinyl, tetrahydropyranyl, azepanyl, piperazinyl, morpholinyl, tetrahydrofuranonyl, tetrahydropyranonyl, pyrrolidinonyl, piperidinonyl, piperazinonyl, morpholinonyl.
Thus, the term “heterocyclyl” includes the following exemplary structures which are not depicted as radicals as each form may be attached through a covalent bond to any atom so long as appropriate valences are maintained:













The term “heteroaryl” means a mono- or polycyclic, preferably mono- or bicyclic-ring system containing one or more heteroatoms selected from N, O or S(O)r with r=0, 1 or 2 wherein at least one of the heteroatoms is part of an aromatic ring, and wherein said ring system may have a carbonyl group. More preferably the term “heteroaryl” as used herein, either alone or in combination with another radical, means a mono- or bicyclic-ring system containing 1, 2, 3 or 4 heteroatoms selected from N, O or S(O)r with r=0, 1 or 2 wherein at least one of the heteroatoms is part of an aromatic ring, and wherein said ring system may have a carbonyl group. The term “heteroaryl” is intended to include all the possible isomeric forms.
Thus, the term “heteroaryl” includes the following exemplary structures which are not depicted as radicals as each form may be attached through a covalent bond to any atom so long as appropriate valences are maintained:







Many of the terms given above may be used repeatedly in the definition of a formula or group and in each case have one of the meanings given above, independently of one another.
Pharmacological Activity
The activity of the compounds of the invention may be demonstrated using the following assay:
The compounds of formula I according to the invention modulate the activity of the G-protein-coupled receptor GPR119. The effect of the compounds on the activation of GPR119 and on the stimulation of intracellular cAMP concentration is determined using the AlphaScreen cAMP Assay Kit (Cat. No. #6760625R) made by PerkinElmer.
MIN6 cells [Miyazaki J et al., Endocrinology. 1990 July; 127(1):126-32] are stably transfected with an expression vector for human GPR119 cDNA (Acc. No. NP—848566). Min-6/hGPR119 cells are cultured in DMEM, 10% FBS, 50 μM β-mercaptoethanol, 0.3 mg/mL Geniticin, 2 mM GlutaMAX at 37° C., 5% CO2. For the assay, the cells are seeded in Optiplates (white, 384-well, 160W-barcoded, TC, sterile with lid, Cat. No. #6007688 (Perkin Elmer); 10000 cells/well; 50 μL). The plates covered with lids are then incubated for 24 hours at 37° C., 5% CO2. After the medium is aspirated from the wells completely, 10 μl of the test compound are added, the compounds are diluted using stimulating buffer (140 mM NaCl, 3.6 mM KCl, 0.5 mM NaH2PO4, 0.5 mM MgSO4, 1.5 mM CaCl2, 10 mM Hepes, 5 mM NaHCO3; pH 7.4. 0.5 mM IBMX and 0.1% BSA, the final DMSO concentration is 1%). After 45 minutes incubation at room temperature (approx. 20° C.), the cAMP concentrations are determined using the AlphaScreen cAMP Assay Kit (Cat. No. #6760625R from PerkinElmer). 10 μl of Biotin-cAMP (final concentration 1 U/well in lysing buffer (5 mM Hepes (pH 7.4), 0.1% BSA, 0.5% Tween) and 10 μL Bead solution (final concentration 1 U/well in lysing buffer) are added. The plates are incubated for another 2 hours at room temperature. The cAMP concentrations are calculated using a cAMP standard curve from the Alpha Screen Counts. The data analysis is carried out by calculating the EC50 value and the maximum value based on a positive control, using suitable software (Graphpad Prism). The compounds according to the invention increase the intracellular cAMP level in the range of 3-5.
The compounds according to the invention typically have EC50 values in the range from about 1 nM to about 10 μM, preferably less than 1 μM, more preferably less than 100 nM.
EC50 values for compounds according to the invention are shown in the following Table. The number of the compound corresponds to the number of the Example in the experimental section.

















Exam-
EC50
Exam-
EC50
Exam-
EC50
Exam-
EC50


ple
[nM]
ple
[nM]
ple
[nM]
ple
[nM]






















1
58
2
62
3
9
4
121


5
263
6
45
7
108
8
281


9
567
10
592
11
310
12
105


13
82
14
9
15
233
16
64


17
51
18
79
19
12
20
13


21
19
22
10
23
86
24
28


25
41
26
18
27
140
28
73









In view of their ability to modulate the activity of the G-protein-coupled receptor GPR119, in particular an agonistic activity, the compounds of general formula I according to the invention, including the compounds I-a, I-b, and I-c, including the corresponding salts thereof, are suitable for the treatment of diseases or conditions which may be affected or which are mediated by the activation of the G-protein-coupled receptor GPR119.
Accordingly, the present invention relates to a compound of general formula I including the compounds I-a, I-b, and I-c as a medicament.
Furthermore, the present invention relates to the use of a compound of general formula I including the compounds I-a, I-b, and I-c or a pharmaceutical composition according to this invention for the treatment and/or prevention of diseases or conditions which are mediated by the activation of the G-protein-coupled receptor GPR119 in a patient, preferably in a human.
In yet another aspect the present invention relates to a method for treating a disease or condition mediated by the activation of the G-protein-coupled receptor GPR119 in a mammal that includes the step of administering to a patient, preferably a human, in need of such treatment a therapeutically effective amount of a compound or a pharmaceutical composition of the present invention including the compounds I-a, I-b, and I-c.
Diseases and conditions mediated by agonists of the G-protein-coupled receptor GPR119 embrace metabolic diseases or conditions.
According to one aspect the compounds and pharmaceutical compositions of the present invention including the compounds I-a, I-b, and I-c are particularly suitable for treating diabetes mellitus, in particular Type 2 diabetes, Type 1 diabetes, complications of diabetes (such as e.g. retinopathy, nephropathy or neuropathies, diabetic foot, ulcers or macroangiopathies), metabolic acidosis or ketosis, reactive hypoglycaemia, hyperinsulinaemia, glucose metabolic disorder, insulin resistance, metabolic syndrome, dyslipidaemias of different origins, atherosclerosis and related diseases, obesity, high blood pressure, chronic heart failure, oedema and hyperuricaemia.
The compounds and pharmaceutical compositions of the present invention including the compounds I-a, I-b, and I-c are also suitable for preventing beta-cell degeneration such as e.g. apoptosis or necrosis of pancreatic beta cells. The compounds and pharmaceutical compositions of the present invention are also suitable for improving or restoring the functionality of pancreatic cells, and also for increasing the number and size of pancreatic beta cells.
Therefore according to another aspect the invention relates to compounds of formula I including the compounds I-a, I-b, and I-c and pharmaceutical compositions according to the invention for use in preventing, delaying, slowing the progression of and/or treating metabolic diseases, particularly in improving the glycaemic control and/or beta cell function in the patient.
In another aspect the invention relates to compounds of formula I including the compounds I-a, I-b, and I-c and pharmaceutical compositions according to the invention for use in preventing, delaying, slowing the progression of and/or treating type 2 diabetes, overweight, obesity, complications of diabetes and associated pathological conditions.
In addition the compounds and pharmaceutical compositions according to the invention including the compounds I-a, I-b, and I-c are suitable for use in one or more of the following therapeutic processes:

    
    
        for preventing, delaying, slowing the progression of or treating metabolic diseases, such as for example type 1 diabetes, type 2 diabetes, insufficient glucose tolerance, insulin resistance, hyperglycaemia, hyperlipidaemia, hypercholesterolaemia, dyslipidaemia, syndrome X, metabolic syndrome, obesity, high blood pressure, chronic systemic inflammation, retinopathy, neuropathy, nephropathy, atherosclerosis, endothelial dysfunction or bone-related diseases (such as osteoporosis, rheumatoid arthritis or osteoarthritis);
        for improving glycaemic control and/or reducing fasting plasma glucose, postprandial plasma glucose and/or the glycosylated haemoglobin HbA1c;
        for preventing, delaying, slowing or reversing the progression of disrupted glucose tolerance, insulin resistance and/or metabolic syndrome to type 2 diabetes;
        for preventing, delaying, slowing the progression of or treating a condition or a disease selected from among the complications of diabetes, such as for example retinopathy, nephropathy or neuropathies, diabetic foot, ulcers or macroangiopathies;
        for reducing weight or preventing weight gain or assisting weight loss;
        for preventing or treating the degradation of pancreatic beta cells and/or improving and/or restoring the functionality of pancreatic beta cells and/or restoring the functionality of pancreatic insulin secretion;
        for maintaining and/or improving insulin sensitivity and/or preventing or treating hyperinsulinaemia and/or insulin resistance.
    
    


In particular, the compounds and pharmaceutical compositions according to the invention including the compounds I-a, I-b, and I-c are suitable for the treatment of obesity, diabetes (comprising type 1 and type 2 diabetes, preferably type 2 diabetes mellitus) and/or complications of diabetes (such as for example retinopathy, nephropathy or neuropathies, diabetic foot, ulcers or macroangiopathies).
The compounds according to the invention including the compounds I-a, I-b, and I-c are most particularly suitable for treating type 2 diabetes mellitus.
The dose range of the compounds of general formula I, including the compounds I-a, I-b, and I-c, applicable per day is usually from 0.001 to 10 mg per kg body weight, for example from 0.01 to 8 mg per kg body weight of the patient. Each dosage unit may conveniently contain from 0.1 to 1000 mg, for example 0.5 to 500 mg.
The actual therapeutically effective amount or therapeutic dosage will of course depend on factors known to those skilled in the art such as age and weight of the patient, route of administration and severity of disease. In any case the compound or composition will be administered at dosages and in a manner which allows a therapeutically effective amount to be delivered based upon patient's unique condition.
The compounds, compositions, including any combinations with one or more additional therapeutic agents, according to the invention may be administered by oral, transdermal, inhalative, parenteral or sublingual route. Of the possible methods of administration, oral or intravenous administration is preferred.
Pharmaceutical Compositions
Suitable preparations for administering the compounds of formula I, including the compounds I-a, I-b, and I-c, optionally in combination with one or more further therapeutic agents, will be apparent to those skilled in the art and include for example tablets, pills, capsules, suppositories, lozenges, troches, solutions, syrups, elixirs, sachets, injectables, inhalatives and powders etc. Oral formulations, particularly solid forms such as e.g. tablets or capsules are preferred. The content of the pharmaceutically active compound(s) is advantageously in the range from 0.1 to 90 wt.-%, for example from 1 to 70 wt.-% of the composition as a whole.
Suitable tablets may be obtained, for example, by mixing one or more compounds according to formula I, including the compounds I-a, I-b, and I-c, with known excipients, for example inert diluents, carriers, disintegrants, adjuvants, surfactants, binders and/or lubricants. The tablets may also consist of several layers. The particular excipients, carriers and/or diluents that are suitable for the desired preparations will be familiar to a person skilled in the art on the basis of his specialist knowledge. The preferred ones are those that are suitable for the particular formulation and method of administration that are desired. The preparations or formulations according to the invention may be prepared using methods known per se that are familiar to a person skilled in the art, such as for example by mixing or combining at least one compound of formula I according to the invention, or a pharmaceutically acceptable salt of such a compound, and one or more excipients, carriers and/or diluents.
Combination Therapy
The compounds of the invention including the compounds I-a, I-b, and I-c may further be combined with one or more, preferably one additional therapeutic agent. According to one embodiment the additional therapeutic agent is selected from the group of therapeutic agents useful in the treatment of diseases or conditions described hereinbefore, in particular associated with metabolic diseases or conditions such as for example diabetes mellitus, obesity, diabetic complications, hypertension, hyperlipidemia. Additional therapeutic agents which are suitable for such combinations include in particular those which for example potentiate the therapeutic effect of one or more active substances with respect to one of the indications mentioned and/or which allow the dosage of one or more active substances to be reduced.
Therefore a compound of the invention including the compounds I-a, I-b, and I-c may be combined with one or more additional therapeutic agents selected from the group consisting of antidiabetic agents, agents for the treatment of overweight and/or obesity and agents for the treatment of high blood pressure, heart failure and/or atherosclerosis.
Antidiabetic agents are for example metformin, sulphonylureas, nateglinide, repaglinide, thiazolidinediones, PPAR-(alpha, gamma or alpha/gamma) agonists or modulators, alpha-glucosidase inhibitors, DPPIV inhibitors, SGLT2-inhibitors, insulin and insulin analogues, GLP-1 and GLP-1 analogues or amylin and amylin analogues, cycloset, 11β-HSD inhibitors. Other suitable combination partners are inhibitors of protein tyrosinephosphatase 1, substances that affect deregulated glucose production in the liver, such as e.g. inhibitors of glucose-6-phosphatase, or fructose-1,6-bisphosphatase, glycogen phosphorylase, glucagon receptor antagonists and inhibitors of phosphoenol pyruvate carboxykinase, glycogen synthase kinase or pyruvate dehydrokinase, alpha2-antagonists, CCR-2 antagonists or glucokinase activators. One or more lipid lowering agents are also suitable as combination partners, such as for example HMG-CoA-reductase inhibitors, fibrates, nicotinic acid and the derivatives thereof, PPAR-(alpha, gamma or alpha/gamma) agonists or modulators, PPAR-delta agonists, ACAT inhibitors or cholesterol absorption inhibitors such as, bile acid-binding substances such as, inhibitors of ileac bile acid transport, MTP inhibitors, or HDL-raising compounds such as CETP inhibitors or ABC1 regulators.
Therapeutic agents for the treatment of overweight and/or obesity are for example antagonists of the cannabinoid1 receptor, MCH-1 receptor antagonists, MC4 receptor agonists, NPY5 or NPY2 antagonists, β3-agonists, leptin or leptin mimetics, agonists of the 5HT2c receptor.
Therapeutic agents for the treatment of high blood pressure, chronic heart failure and/or atherosclerosis are for example A-II antagonists or ACE inhibitors, ECE inhibitors, diuretics, β-blockers, Ca-antagonists, centrally acting antihypertensives, antagonists of the alpha-2-adrenergic receptor, inhibitors of neutral endopeptidase, thrombocyte aggregation inhibitors and others or combinations thereof are suitable. Angiotensin II receptor antagonists are preferably used for the treatment or prevention of high blood pressure and complications of diabetes, often combined with a diuretic such as hydrochlorothiazide.
The dosage for the combination partners mentioned above is usually 1⁄5 of the lowest dose normally recommended up to 1/1 of the normally recommended dose.
Preferably, compounds of the present invention including the compounds I-a, I-b, and I-c and/or pharmaceutical compositions comprising a compound of the present invention optionally in combination with one or more additional therapeutic agents are administered in conjunction with exercise and/or a diet.
Therefore, in another aspect, this invention relates to the use of a compound according to the invention including the compounds I-a, I-b, and I-c in combination with one or more additional therapeutic agents described hereinbefore and hereinafter for the treatment of diseases or conditions which may be affected or which are mediated by the activation of the G-protein-coupled receptor GPR119, in particular diseases or conditions as described hereinbefore and hereinafter.
In yet another aspect the present invention relates to a method for treating a disease or condition mediated by the activation of the G-protein-coupled receptor GPR119 in a patient that includes the step of administering to the patient, preferably a human, in need of such treatment a therapeutically effective amount of a compound of the present invention including the compounds I-a, I-b, and I-c in combination with a therapeutically effective amount of one or more additional therapeutic agents described in hereinbefore and hereinafter.
The use of the compound according to the invention including the compounds I-a, I-b, and I-c in combination with the additional therapeutic agent may take place simultaneously or at staggered times.
The compound according to the invention including the compounds I-a, I-b, and I-c and the one or more additional therapeutic agents may both be present together in one formulation, for example a tablet or capsule, or separately in two identical or different formulations, for example as a so-called kit-of-parts.
Consequently, in another aspect, this invention relates to a pharmaceutical composition which comprises a compound according to the invention including the compounds I-a, I-b, and I-c and one or more additional therapeutic agents described hereinbefore and hereinafter, optionally together with one or more inert carriers and/or diluents.
Other features and advantages of the present invention will become apparent from the following more detailed Examples which illustrate, by way of example, the principles of the invention.
EXAMPLES
The terms “ambient temperature” and “room temperature” are used interchangeably and designate a temperature of about 20° C.
Preliminary Remarks:
As a rule, 1H-NMR and/or mass spectra have been obtained for the compounds prepared. The Rf values are determined using Merck silica gel 60 F254 plates and UV light at 254 nm.
Parameters of analytical HPLC employed for characterization of products (TFA denotes trifluoroacetic acid):
















Method:
1



Device:
Agilent 1200 with DA and MS detector



Column:
XBridge C18, 3 × 30 mm, 2.5 μm



Column Supplier:
Waters















Gradient/






Solvent
% Solvent
% Solvent
Flow
Temperature


Time [min]
[H2O, 0.1% TFA]
[Methanol]
[ml/min]
[° C.]





0.0 
95
5
2.2
60


0.05
95
5
2.2
60


1.40
0
100
2.2
60


1.80
0
100
2.2
60

























Method:
2



Device:
Waters Alliance with DA and MS detector



Column:
XBridge C18, 4.6 × 30 mm, 3.5 μm



Column Supplier:
Waters















Gradient/






Solvent
% Solvent
% Solvent
Flow
Temperature


Time [min]
[H2O, 0.1% TFA]
[Methanol]
[ml/min]
[° C.]





0.0
95
5
4.8
60


1.6
0
100
4.8
60


 1.85
0
100
4.8
60


1.9
95
5
4.8
60

























Method:
3



Device:
Waters Alliance with DA and MS detector



Column:
XBridge C18, 4.6 × 30 mm, 3.5 μm



Column Supplier:
Waters















Gradient/






Solvent
% Solvent
% Solvent
Flow
Temperature


Time [min]
[H2O, 0.1% TFA]
[Methanol]
[ml/min]
[° C.]





0.0
95
5
4
60


1.6
0
100
4
60


 1.85
0
100
4
60


1.9
95
5
4
60

























Method:
4



Device:
Agilent 1100 with DA and MS detector



Column:
XBridge C18, 4.6 × 30 mm, 3.5 μm



Column Supplier:
Waters















Gradient/
% Solvent





Solvent
[H2O,
% Solvent
Flow
Temperature


Time [min]
0.1% HCOOH]
[Methanol]
[ml/min]
[° C.]





0.0 
95
5
4
60


0.15
95
5
4
60


1.7 
0
100
4
60


2.25
0
100
4
60

























Method:
5



Device:
Agilent 1200 with DA and MS detector



Column:
XBridge C18, 3 × 30 mm, 2.5 μm



Column Supplier:
Waters















Gradient/






Solvent
% Solvent
% Solvent
Flow
Temperature


Time [min]
[H2O, 0.1% NH3]
[Methanol]
[ml/min]
[° C.]





0.0 
95
5
2.2
60


0.05
95
5
2.2
60


1.40
0
100
2.2
60


1.80
0
100
2.2
60

























Method:
6



Device:
Agilent 1100 with DA and MS detector



Column:
XBridge C18, 4.6 × 30 mm, 3.5 μm



Column Supplier:
Waters















Gradient/






Solvent
% Solvent
% Solvent
Flow
Temperature


Time [min]
[H2O, 0.1% NH3]
[Methanol]
[ml/min]
[° C.]





0.0 
95
5
4
60


0.15
95
5
4
60


1.7 
0
100
4
60


2.25
0
100
4
60









Intermediate 1
4-(5-Chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester




Copper(I) iodide (25 mg) and bis-(triphenylphosphine)-palladium(II)-chloride (30 mg) are added to 6-chloro-4-iodo-pyridin-3-ol (200 mg) in N,N-dimethylformamide (3 mL) under Ar atmosphere at room temperature. Triethylamine (110 μL) is added and the resulting mixture is stirred at room temperature for 1 h. 4-Ethynyl-piperidine-1-carboxylic acid tert-butyl ester (175 mg) dissolved in N,N-dimethylformamide (2 mL) is added dropwise and the mixture is stirred at 55° C. for 3 h. The solvent is evaporated and the residue is chromatographed on silica gel (cyclohexane/ethyl acetate 70:30) to give the title compound. LC (method 2): tR=1.40 min; Mass spectrum (ESI+): m/z=337/339 (Cl) [M+H]+.
Intermediate 2
4-(2,5-Dichloro-pyridin-4-ylethynyl)-piperidine-1-carboxylic acid tert-butyl ester




Copper(I) iodide (0.50 g), bis-(triphenylphosphine)-palladium(II) chloride (0.50 g), and triethylamine (3.4 mL) are added successively to a solution of 2,5-dichloro-4-iodo-pyridine (6.54 g) in N,N-dimethylformamide (45 mL) under Ar atmosphere at room temperature. The resulting mixture is stirred at room temperature for 1 h prior to the addition of 4-ethynyl-piperidine-1-carboxylic acid tert-butyl ester (5.15 g). The mixture is stirred at 55° C. for 2 h. The solvent is evaporated and the residue is diluted with ethyl acetate. The resulting mixture is washed with water and brine, dried (Na2SO4), and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 85:15→50:50) to give the title compound. LC (method 3): tR=1.66 min; Mass spectrum (ESI+): m/z=355/357/359 (2 Cl) [M+H]+.
Intermediate 3
4-[5-Chloro-2-(4-cyano-2-fluoro-phenyl)-pyridin-4-ylethynyl]-piperidine-1-carboxylic acid tert-butyl ester




A flask charged with a stir bar, 4-(2,5-dichloro-pyridin-4-ylethynyl)-piperidine-1-carboxylic acid tert-butyl ester (0.30 g), 4-cyano-2-fluoro-phenylboronic acid (0.20 g), 2 M aqueous Na2CO3 solution (1 mL), and 1,4-dioxane (3 mL) is sparged with Ar for 10 min. Tetrakis(triphenylphosphine)palladium(0) (40 mg) is added and the resulting mixture is stirred in a microwave over at 160° C. for 45 min. After cooling to room temperature, water is added and the resulting mixture is extracted with ethyl acetate.
The combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 85:15) to give the title compound. LC (method 3): tR=1.70 min; Mass spectrum (ESI+): m/z=440/442 (Cl) [M+H]+.
Intermediate 4
4-[5-(2-Triisopropylsilanyl-oxazol-5-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




A mixture of 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (0.20 g), 5-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-2-triisopropylsilanyl-oxazole (0.35 g), 2 M aqueous Na2CO3 solution (0.8 mL), and N,N-dimethylformamide (3 mL) is sparged with argon for 10 min. PdCl2[1,1′-bis(diphenylphosphino)-ferrocene]*CH2Cl2 complex (0.15 g) is added and the mixture is stirred at 80° C. overnight. The mixture is concentrated, diluted with water and methanol, and purified by HPLC on reversed phase (methanol/water) to give the title compound. LC (method 4): tR=2.07 min; Mass spectrum (ESI+): m/z=526 [m+H]+.
Intermediate 5
5-(2-Fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine




Trifluoroacetic acid (1.2 mL) is added to 4-[5-(2-fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester (0.78 g) in dichloromethane (14 mL). The mixture is stirred at room temperature overnight. Aqueous NaHCO3 solution is added, the resulting mixture is stirred for 5 min, and the phases are separated. The aqueous phase is extracted with dichloromethane and the combined organic phases are washed with water and dried (Na2SO4). The solvent is evaporated to give the title compound. LC (method 1): tR=0.59 min; Mass spectrum (ESI+): m/z=375 [M+H]+.
Intermediate 6
1-{4-[5-(2-Fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidin-1-yl}-2-methyl-propan-2-ol




1,1-Dimethyloxirane (55 μL) is added to a solution of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine (0.15 g) in methanol (2 mL) and dichloromethane (3 mL) at room temperature. The solution is stirred at 50° C. overnight. The solution is concentrated to give the crude title compound that is used without further purification. LC (method 4): tR=0.98 min; Mass spectrum (ESI+): m/z=447 [M+H]+.
Intermediate 7
5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine




The title compound is prepared from 4-[5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester following a procedure analogous to that described for Intermediate 5.
Intermediate 8
4-(5-Chloro-furo[2,3-c]pyridin-2-yl)-4-methyl-piperidine-1-carboxylic acid tert-butyl ester




Step 1: 4-formyl-4-methyl-piperidine-1-carboxylic acid tert-butyl ester
Potassium tert-butoxide (2.80 g) and methyl iodide (3.60 mL) are added to an ice cold solution of 4-formyl-piperidine-1-carboxylic acid tert-butyl ester (4.00 g) in dichloromethane (24 mL). The mixture is stirred in the cooling bath for 30 min and then at room temperature overnight. Brine is added and the resulting mixture is extracted with dichloromethane. The combined extracts are dried (MgSO4) and concentrated to give the crude title compound that is used without further purification.
Step 2: 4-ethynyl-4-methyl-piperidine-1-carboxylic acid tert-butyl ester
K2CO3 (5.25 g) and 4-formyl-4-methyl-piperidine-1-carboxylic acid tert-butyl ester (5.23 g) are added to an ice cold mixture of dimethyl (1-diazo-2-oxopropyl)-phosphonate (5.50 g), molecular sieves (2 g), and methanol (30 mL). The mixture is stirred in the cooling bath for 30 min and then at room temperature overnight. The mixture is filtered and aqueous NH4Cl solution is added to the filtrate. The resulting mixture is extracted with dichloromethane and the combined extracts are concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 4:1→1:1) to give the title compound.
Step 3: 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-4-methyl-piperidine-1-carboxylic acid tert-butyl ester
The title compound is prepared from 4-ethynyl-4-methyl-piperidine-1-carboxylic acid tert-butyl ester and 2-chloro-5-hydroxy-4-iodo-pyridine following a procedure analogous to that described for Intermediate 5. LC (method 4): tR=1.76 min; Mass spectrum (ESI+): m/z=351/353 (Cl) [M+H]+.
Example 1
4-[5-(4-Methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




A mixture of 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (390 mg), 4-(methanesulfonyl)phenyl boronic acid (350 mg), 2 M aqueous Na2CO3 solution (1.50 mL), and 1,4-dioxane (10 mL) is sparged with Ar for 10 min. Pd(PPh3)4 (100 mg) is added and the resulting mixture is stirred at 170° C. for 1 h in a microwave oven. The reaction mixture is concentrated, diluted with water, and extracted with ethyl acetate. The combined extracts are washed with brine, dried (MgSO4), and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 60:40→40:60) to give the title compound. LC (method 2): tR=1.23 min; Mass spectrum (ESI+): m/z=457 [M+H]+.
Example 2
4-[5-(3-Fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 3-fluoro-4-methylsulfonyl-phenylboronic acid following a procedure analogous to that described for Example 1. LC (method 4): tR=1.71 min; Mass spectrum (ESI+): m/z=475 [M+H]+.
Example 3
4-[5-(2-Fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




A mixture of 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (100 mg), 2-fluoro-4-(methanesulfonyl)phenylboronic acid (97 mg), 2 M aqueous Na2CO3 solution (0.33 mL), methanol (0.5 mL), and 1,4-dioxane (1.5 mL) is sparged with Ar for 10 min. PdCl2 [1,1′-bis(diphenylphosphino)-ferrocene]*CH2Cl2 complex (22 mg) is added and the mixture is stirred at reflux temperature for 2 h. The mixture is diluted with water and methanol and purified by HPLC on reversed phase (methanol/water) to give the title compound. LC (method 5): tR=1.29 min; Mass spectrum (ESI+): m/z=475 [M+H]+.
Example 4
4-[5-(4-Methanesulfonylamino-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 4-methylsulfonylamino-phenylboronic acid following a procedure analogous to that described for Example 1. LC (method 4): tR=1.57 min; Mass spectrum (ESI+): m/z=472 [M+H]+.
Example 5
4-[5-(4-Cyano-3-fluoro-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 4-cyano-3-fluoro-phenylboronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 4): tR=1.84 min; Mass spectrum (ESI+): m/z=422 [M+H]+.
Example 6
4-[5-(4-Ethylcarbamoyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 4-ethylcarbamoyl-phenylboronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 4): tR=1.68 min; Mass spectrum (ESI+): m/z=450 [M+H]+.
Example 7
4-[5-(2-Fluoro-4-aminocarbonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




A flask is charged under Ar atmosphere with a stir bar, 4-[5-chloro-2-(4-cyano-2-fluoro-phenyl)-pyridin-4-ylethynyl]-piperidine-1-carboxylic acid tert-butyl ester (110 mg), powdered KOH (42 mg), 2-di-tert-butylphosphino-2,4,6-triisopropylbiphenyl (8 mg), tris(dibenzylideneacetone)dipalladium(0) chloroform adduct (6 mg), water (1.5 mL), and 1,4-dioxane (3 mL). The mixture is heated to 110° C. and stirred at this temperature for 2 h. After cooling the mixture to room temperature, water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is chromatographed on silica gel [dichloromethane/(dichloromethane/methanol/7 M ammonia in methanol 50:48:2) 9:1] to give the title compound. LC (method 3): tR=1.26 min; Mass spectrum (ESI+): m/z=440 [M+H]+.
Example 8
4-[5-(4-Cyano-2-fluoro-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




Trifluoroacetic anhydride (26 μL) is added to a solution of 4-[5-(2-fluoro-4-aminocarbonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester (40 mg) and triethylamine (51 μL) in dichloromethane (3 mL) at room temperature. The solution is stirred at room temperature for 2 h before another portion of trifluoroacetic anhydride (26 μL) and triethylamine (51 μL) is added. The solution is stirred for 0.5 h and then aqueous NaHCO3 solution is added. The resulting mixture is extracted with dichloromethane and the combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is triturated with ether and then with methanol to give the title compound. LC (method 3): tR=1.52 min; Mass spectrum (ESI+): m/z=422 [M+H]+.
Example 9
4-[5-(4-Benzyloxycarbonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 4-benzyloxycarbonyl-phenylboronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. Mass spectrum (ESI+): m/z=513 [M+H]+.
Example 10
4-(5-Oxazol-5-yl-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester




Tetrabutylammonium fluoride (1 mol/L in tetrahydrofuran; 1.5 mL) is added to a solution of 4-[5-(2-triisopropylsilanyl-oxazol-5-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester (0.31 g) in tetrahydrofuran (6 mL) chilled in an ice bath. The cooling bath is removed and the solution is stirred at room temperature for 2 h. Water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are dried (Na2SO4) and concentrated. The residue is purified by HPLC on reversed phase (methanol/water) to give the title compound. LC (method 4): tR=1.66 min; Mass spectrum (ESI+): m/z=370 [M+H]+.
Example 11
4-(5-Pyridin-4-yl-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and pyridine-4-boronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 1): tR=1.31 min; Mass spectrum (ESI+): m/z=380 [M+H]+.
Example 12
4-[5-(4-Methanesulfonylmethyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 4-methylsulfonylmethyl-phenylboronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 1): tR=1.26 min; Mass spectrum (ESI+): m/z=471 [M+H]+.
Example 13
4-[5-(4-Methanesulfonyl-piperazin-1-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




4-(5-Chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (80 mg) is added to a flask charged with a stir bar, N-methylsulfonyl-piperazine (47 mg), potassium tert-butoxide (63 mg), Xantphos (103 mg), tris(dibenzylideneacetone)-dipalladium(0) (54 mg), and toluene (3 mL). The mixture is sparged with Ar at room temperature for 10 min and then stirred at 110° C. overnight. After cooling the mixture to room temperature, water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are dried (Na2SO4) and concentrated. The residue is purified by HPLC on reversed phase (methanol/water) to give the title compound. LC (method 5): tR=1.25 min; Mass spectrum (ESI+): m/z=465 [M+H]+.
Example 14
4-[5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 1-methanesulfonyl-4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-1,2,3,6-tetrahydro-pyridine following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 1): tR=1.27 min; Mass spectrum (ESI+): m/z=462 [M+H]+.
Example 15
4-[5-(3-Fluoro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-piperidine-1-carboxylic acid tert-butyl ester and 3-fluoro-pyridine-4-boronic acid following a procedure analogous to that described for Example 3. LC (method 5): tR=1.32 min; Mass spectrum (ESI+): m/z=398 [M+H]+.
Example 16
2-[1-(5-Ethyl-pyrimidin-2-yl)-piperidin-4-yl]-5-(2-fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridine




A mixture of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine (70 mg), 2-chloro-5-ethyl-pyrimidine (25 mg), N,N-diisopropyl-ethylamine (67 μL), and N,N-dimethylformamide (1 mL) is stirred at 120° C. for 4 h and then at 100° C. overnight. After cooling to room temperature, water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 4:1→1:1) to give the title compound. LC (method 1): tR=1.18 min; Mass spectrum (ESI+): m/z=481 [M+H]+.
Example 17
5-(2-Fluoro-4-methanesulfonyl-phenyl)-2-[1-(1-trifluoromethyl-cyclopropylmethyl)-piperidin-4-yl]-furo[2,3-c]pyridine




A mixture of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine (80 mg), methanesulfonic acid 1-trifluoromethyl-cyclopropylmethyl ester (120 mg), KI (21 mg), K2CO3 (92 mg), and N-methylpyrrolidinone (3 mL) is stirred at 100° C. for 4 h. After cooling to room temperature, water is added and the mixture is extracted with ethyl acetate. The combined extracts are dried (Na2SO4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 4:1→1:1) to give the title compound. Mass spectrum (ESI+): m/z=497 [M+H]+.
Example 18
5-(2-Fluoro-4-methanesulfonyl-phenyl)-2-[1-(2-fluoro-2-methyl-propyl)-piperidin-4-yl]-furo[2,3-c]pyridine




Bis(2-methoxyethyl)aminosulfur trifluoride (50% in toluene; 0.27 mL) is added to a solution of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-[1-(2-hydroxy-2-methyl-propyl)-piperidin-4-yl]-furo[2,3-c]pyridine (0.19 g) in dichloromethane (3 mL) chilled in an ice bath. The solution is stirred at room temperature overnight. The solution is diluted with dichloromethane and little methanol and washed with aqueous NaHCO3 solution. The solution is dried (Na2SO4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 1:1→1:2) to give the title compound. LC (method 4): tR=1.09 min; Mass spectrum (ESI+): m/z=449 [M+H]+.
Example 19
4-[5-(2-Fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid isopropyl ester




Isopropyl chloroformate (1 mol/L in toluene; 0.30 mL) is added to a solution of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine (75 mg) and N,N-diisopropyl-ethylamine (0.10 mL) in tetrahydrofuran (1 mL) chilled in an ice bath. The solution is stirred at room temperature for 3 h. The solution is concentrated and the residue is taken up in water and dichloromethane. The resulting mixture is extracted with dichloromethane and the combined extracts are dried (Na2SO4). The solvent is evaporated and the residue is purified by HPLC on reversed phase (methanol/water) to give the title compound. Mass spectrum (ESI+): m/z=461 [M+H]+.
Example 20
4-[5-(2-Fluoro-4-methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid 1-methyl-cyclopropyl ester




1-Methyl-cyclopropyl 4-nitrophenyl carbonate (48 mg) is added to a solution of 5-(2-fluoro-4-methanesulfonyl-phenyl)-2-piperidin-4-yl-furo[2,3-c]pyridine (75 mg) and N,N-diisopropyl-ethylamine (32 μL) in tetrahydrofuran (1 mL) at room temperature. The mixture is stirred at room temperature overnight. The solution is concentrated and the residue is taken up in ethyl acetate. The resulting mixture is washed with 1 M aqueous NaOH solution (3×), water, and brine. The organic phase is dried (Na2SO4) and concentrated. The residue is purified by HPLC on reversed phase (methanol/water) to give the title compound. Mass spectrum (ESI+): m/z=473 [M+H]+.
Example 21
4-[5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid isopropyl ester




The title compound is prepared from 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine and isopropyl chloroformate following a procedure analogous to that described for Example 19. LC (method 6): tR=1.45 min; Mass spectrum (ESI+): m/z=448 [M+H]+.
Example 22
4-[5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-piperidine-1-carboxylic acid 1-methyl-cyclopropyl ester




The title compound is prepared from 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine and 1-methyl-cyclopropyl 4-nitrophenyl carbonate following a procedure analogous to that described for Example 20. Mass spectrum (ESI+): m/z=460 [M+H]+.
Example 23
4-[5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-5′-trifluoromethyl-3,4,5,6-tetrahydro-2H-[1,2′]bipyridinyl




A mixture of 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine (50 mg), 2-chloro-5-trifluoromethyl-pyridine (25 mg), K2CO3 (40 mg), and N-methylpyrrolidinone (1 mL) is stirred at 60° C. overnight. After cooling to room temperature, water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 4:1→1:2) to give the title compound. LC (method 1): tR=1.05 min; Mass spectrum (ESI+): m/z=507 [M+H]+.
Example 24
5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-[1-(1-trifluoromethyl-cyclopropylmethyl)-piperidin-4-yl]-furo[2,3-c]pyridine




The title compound is prepared from 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine and methanesulfonic acid 1-trifluoromethyl-cyclopropylmethyl ester following a procedure analogous to that described for Example 17. LC (method 1): tR=0.65 min; Mass spectrum (ESI+): m/z=484 [M+H]+.
Example 25
2-[1-(5-Chloro-pyrimidin-2-yl)-piperidin-4-yl]-5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridine




The title compound is prepared from 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine and 2,5-dichloropyrimidine following a procedure analogous to that described for Example 23. LC (method 1): tR=1.07 min; Mass spectrum (ESI+): m/z=474/476 (Cl) [M+H]+.
Example 26
5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-[1-(5-trifluoromethyl-[1,3,4]thiadiazol-2-yl)-piperidin-4-yl]-furo[2,3-c]pyridine




A mixture of 5-(1-methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-2-piperidin-4-yl-furo[2,3-c]pyridine (HCl salt, 70 mg), 2-chloro-5-trifluoromethyl-[1,3,4]thiadiazole (35 mg), K2CO3 (55 mg), and dimethyl sulfoxide (3 mL) is stirred at 60° C. overnight. After cooling to room temperature, water is added and the resulting mixture is extracted with ethyl acetate. The combined extracts are washed with brine, dried (Na2SO4), and concentrated. The residue is triturated with methanol and then with diisopropylether to give the title compound. LC (method 1): tR=0.94 min; Mass spectrum (ESI+): m/z=514 [M+H]+.
Example 27
4-[5-(4-Methanesulfonyl-phenyl)-furo[2,3-c]pyridin-2-yl]-4-methyl-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-4-methyl-piperidine-1-carboxylic acid tert-butyl ester and 4-methanesulfonyl-phenylboronic acid following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 4): tR=1.71 min; Mass spectrum (ESI+): m/z=471 [M+H]+.
Example 28
4-[5-(1-Methanesulfonyl-1,2,3,6-tetrahydro-pyridin-4-yl)-furo[2,3-c]pyridin-2-yl]-4-methyl-piperidine-1-carboxylic acid tert-butyl ester




The title compound is prepared from 4-(5-chloro-furo[2,3-c]pyridin-2-yl)-4-methyl-piperidine-1-carboxylic acid tert-butyl ester and 1-methanesulfonyl-4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-1,2,3,6-tetrahydro-pyridine following a procedure analogous to that described for Example 1; the reaction is conducted at 150° C. LC (method 4): tR=1.58 min; Mass spectrum (ESI+): m/z=476 [M+H]+.

## Claims

1. A compound of formula I



wherein:
R1 is a group consisting of:
a 5- or 6-membered heteroaromatic ring which contains 1, 2, or 3 heteroatoms selected independently from N, O, and S, and optionally substituted with a group RC and/or one or more substituents independently selected from LA;
a group C(═O)—O—Ra, wherein Ra is C1-6-alkyl optionally mono- or polysubstituted with fluorine or C3-6-cycloalkyl optionally mono- or polysubstituted with fluorine and optionally substituted with CH3, CF3, or CHF2; and
a group CH2—Rb, wherein Rb is C1-6-alkyl optionally mono- or polysubstituted with fluorine, or C3-6-cycloalkyl optionally mono- or polysubstituted with fluorine and optionally substituted with CH3, CF3, or CHF2;

R2 is H or C1-3-alkyl;
A is a group consisting of:
1,2,3,6-tetrahydropyridin-4-yl and piperazin-1-yl, each substituted at the N with a C1-4-alkyl-S(═O)2— group; and
a phenyl ring and a 5- or 6-membered heteroaromatic ring which contains 1, 2, or 3 heteroatoms selected independently from N, O, and S, each optionally substituted with a group T and optionally substituted with one or more substituents independently selected from LA;

T is a group consisting of F, Cl, Br, I, CN, OH, NO2, C1-6-alkyl, C2-6-alkenyl, C2-6-alkynyl, C3-6-cycloalkyl, C1-6-alkyl-O—, C3-6-cycloalkyl-O—, C1-6-alkyl-S—, HO—C(═O)—, C1-6-alkyl-O—C(═O)—, C1-4-alkyl-C(═O)—, C3-6-cycloalkyl-C(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—(RN)N—, heterocyclyl, heterocyclyl-O—, aryl, aryl-O—, heteroaryl, and heteroaryl-O—, wherein:
each alkyl, alkenyl, alkynyl, and cycloalkyl group thereof is optionally substituted with one or more substituents independently selected from F, Cl, CN, OH, C1-3-alkyl, C3-6-cycloalkyl, C1-3-alkyl-O—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, aryl, heteroaryl, or heterocyclyl, and
heterocyclyl is a 4- to 7-membered unsaturated or saturated carbocyclic ring in which 1 or 2-CH2-groups independently of each other are replaced by NRN, O, —C(═O)—, S, —S(═O)—, or —S(═O)2—, and/or in which a —CH-group is replaced by N;

RN is independently H, C1-4-alkyl, C1-4-alkyl-C(═O)—, or C1-4-alkyl-S(═O)2—;
RNT1 is H, C1-6-alkyl, C3-6-cycloalkyl, C1-6-alkyl-C(═O)—, C1-6-alkyl-S(═O)2, heterocyclyl, aryl, or heteroaryl, wherein:
each alkyl and cycloalkyl group thereof is optionally substituted with one or more substituents independently selected from F, OH, CN, C1-4-alkyl, C1-4-alkyl-O—, RN2N, C1-4-alkyl-S(═O)2—, C3-6-cycloalkyl, heterocyclyl, phenyl, or heteroaryl,
heterocyclyl is optionally substituted with one or more substituents independently selected from F, C1-4-alkyl, RN2N, OH, or C1-4-alkyl-O—,
heterocyclyl is a C4-7-cycloalkyl ring in which 1 or 2-CH2-groups are independently replaced by NRN, O, C(═O), S, S(═O), or S(═O)2,
heteroaryl is a 5- or 6-membered aromatic ring which contains 1, 2, or 3 heteroatoms independently selected from N, O, and S, wherein the H-atom in one or more NH groups is replaced by RN, and

RNT2 is H or C1-6-alkyl; or
RNT1 and RNT2 are linked to form one group selected from a group consisting of a C3-5-alkylene group, wherein 1 or 2-CH2-groups are independently replaced by NRN, O, C(═O), S, S(═O), or S(═O)2, and which are optionally substituted with one or more substituents independently selected from F, C1-4-alkyl, (RN)2N, OH, or C1-4-alkyl-O—;
LA is a group consisting of F, Cl, Br, CN, OH, NO2, C1-4-alkyl-O—, (RN)2N—C(═O), (RN)2N—, and C1-4-alkyl-S(═O)2—, wherein each alkyl group is optionally substituted with one or more substituents independently selected from F, Cl, CN, OH, or C1-3-alkyl-O—; and
RC is a group consisting of F, Cl, Br, I, CN, OH, NO2, C1-6-alkyl, C2-6-alkenyl, C2-6-alkynyl, C3-6-cycloalkyl, C1-6-alkyl-O—, C3-6-cycloalkyl-O—, C1-6-alkyl-S—, HO—C(═O)—, C1-6-alkyl-O—C(═O)—, C1-4-alkyl-C(═O)—, C3-6-cycloalkyl-C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—(RN)N—, heterocyclyl, heterocyclyl-O—, aryl, aryl-O—, heteroaryl, and heteroaryl-O—, wherein:
each alkyl, alkenyl, alkynyl, and cycloalkyl group is optionally substituted with one or more substituents independently selected from F, Cl, CN, OH, C1-3-alkyl, C3-6-cycloalkyl, C1-3-alkyl-O—, RNT1RNT2N—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, aryl, heteroaryl, or heterocyclyl, and
heterocyclyl is a 4- to 7-membered unsaturated or saturated carbocyclic ring in which 1 or 2-CH2— groups are independently replaced by NRN, O, —C(═O)—, S, —S(═O)—, or —S(═O)2—, and/or in which a —CH-group is replaced by N; and

wherein:
aryl is phenyl or naphthyl,
heteroaryl is a 5- or 6-membered aromatic ring which contains 1, 2, 3, or 4 heteroatoms independently selected from N, O, or S, wherein the H-atom in one or more NH groups is optionally replaced by RN; and
each aryl, heteroaryl, or heterocyclyl group is optionally substituted with one or more substituents independently selected from LA,

or a salt thereof.

2. The compound according to claim 1, wherein the compound does not include the compounds I-a, I-b, and I-c:

3. The compound according to claim 1, wherein R1 is selected from the group consisting of:
a) a group selected from:




 wherein each ring is optionally substituted with one substituent RC, and
b) a group selected from —C(═O)—O—(C1-4-alkyl) and




 wherein each alkyl residue is optionally substituted with one or more fluorine atoms, and
c) a group selected from

4. The compound according to claim 1, wherein A is selected from the group consisting of:




optionally additionally substituted with one or two F atoms, and
b)

5. The compound according to claim 1, wherein T is —CN, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)2—(RN)N—, or RNT1RNT2N—.

6. The compound according to claim 3, wherein T is —CN, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)2—(RN)N—, or RNT1RNT2N—.

7. The compound according to claim 4, wherein T is —CN, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—S(═O)2—, RNT1RNT2N—C(═O)—, C1-4-alkyl-S(═O)2—(RN)N—, or RNT1RNT2N—.

8. The compound according to claim 1, wherein RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—.

9. The compound according to claim 3, wherein RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—.

10. The compound according to claim 4, wherein RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—.

11. The compound according to claim 5, wherein RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—.

12. The compound according to claim 1, wherein:
R1 is selected from the group consisting of:
a) a group selected from:




 wherein each ring is optionally substituted with one substituent RC and RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—,
b) a group selected from:




 wherein each alkyl residue is optionally substituted with one or more fluorine atoms, and
c) a group selected from




R2 is H; and
A is selected from the group consisting of:




 wherein T is NC—, C1-4-alkyl-S(═O)2—CH2—, C1-4-alkyl-S(═O)2—, RNT1RNT2N—C(═O)—, or C1-4-alkyl-S(═O)2—NH—, and optionally additionally substituted with one or two F atoms, and




or a pharmaceutically acceptable salt thereof.

13. The compound according to claim 1, wherein:
R1 is selected from the group consisting of:




 wherein each ring is optionally substituted with one substituent RC and RC is Cl, C1-4-alkyl, F3C—, C3-4-cycloalkyl, or C1-3-alkyl-O—;




 wherein each alkyl residue is optionally substituted with one or more fluorine atoms; and




R2 is H; and
A is selected from the group consisting of:




or a pharmaceutically acceptable salt thereof.

14. A pharmaceutical composition comprising an effective amount of the compound according to claim 1 and an inert carrier or diluent.

15. A method for treating diabetes in a patient in need thereof, the method comprising administering to the patient a pharmaceutically effective amount of the compound according to claim 1.