Attachment F EDSP ICR Addendum (EPA ICR #2488.01; OMB # 2070-[new])

Assay

2009 Assay (a-1) Adjusted

Assay (a-2) Paperwork

Burden (b) Management

Burden (c) Technical

Burden (d) Clerical

Burden (e) Mgmt (f) Technical (g) Clerical (h) Total (i)

Amphibian Metamorphosis (Frog) 82,065.50 $ 88,065.00 $ 30,822.75 $ 6,164.55 $ 20,034.79 $ 4,623.41 $ 49.67 327.26 125.64 502.57

Androgen Receptor Binding (Rat Prostrate) 23,814.00 $ 25,554.95 $ 8,944.23 $ 1,788.85 $ 5,813.75 $ 1,341.63 $ 14.41 94.96 36.46 145.83

Aromatase (Human Recombinant) 29,800.00 $ 31,978.57 $ 11,192.50 $ 2,238.50 $ 7,275.13 $ 1,678.88 $ 18.04 118.84 45.62 182.50

Estrogen Receptor Binding (Rat Uterine) 23,329.00 $ 25,034.50 $ 8,762.08 $ 1,752.42 $ 5,695.35 $ 1,314.31 $ 14.12 93.03 35.71 142.86

Estrogen Receptor Transcriptional Activation

(Human Cell Line) 23,867.00 $ 25,611.83 $ 8,964.14 $ 1,792.83 $ 5,826.69 $ 1,344.62 $ 14.45 95.18 36.54 146.17

Fish Short-term Reproduction 111,230.50 $ 119,362.15 $ 41,776.75 $ 8,355.35 $ 27,154.89 $ 6,266.51 $ 67.32 443.56 170.29 681.17

Hershberger (Rat) 45,583.00 $ 48,915.40 $ 17,120.39 $ 3,424.08 $ 11,128.25 $ 2,568.06 $ 27.59 181.77 69.78 279.14

Female Pubertal (Rat) 96,967.00 $ 104,055.90 $ 36,419.57 $ 7,283.91 $ 23,672.72 $ 5,462.94 $ 58.69 386.68 148.45 593.82

Male Pubtertal (Rat) 100,350.00 $ 107,686.21 $ 37,690.17 $ 7,538.03 $ 24,498.61 $ 5,653.53 $ 60.74 400.17 153.63 614.54

Steroidogenesis (Human Cell Line) 17,450.00 $ 18,725.70 $ 6,554.00 $ 1,310.80 $ 4,260.10 $ 983.10 $ 10.56 69.59 26.71 106.86

Uterotrophic (Rat) 41,242.00 $ 44,257.05 $ 15,489.97 $ 3,097.99 $ 10,068.48 $ 2,323.50 $ 24.96 164.46 63.14 252.56

Total Burden and Costs: 595,698.00 $ 639,247.26 $ 223,736.55 $ 44,747.31 $ 145,428.76 $ 33,560.49 $ 360.55 2,375.50 911.97 3,648.02

(b) Based on 35% of the total estimated test costs. 35%

(c) Calculated as 20% of (b), which is 35% of the total estimated test costs. 20%

(d) Calculated as 65% of (b), which is 35% of the total estimated test costs. 65% Addendum

(e) Calculated as 15% of (b), which is 35% of the total estimated test costs. 15% Original Draft Final Final

(f) Based on total estimated managerial costs (j) divided by the loaded labor rate for managers. 103.62 $ 112.49 $ 122.34 $ 124.11 $

(g) Based on total estimated technical costs (j) divided by the loaded labor rate for technical staff. 76.92 $ 55.49 $ 60.35 $ 61.22 $

(h) Based on total estimated clerical costs (j) divided by the loaded labor rate for clerical staff. 33.60 $ 33.35 $ 36.27 $ 36.80 $

(i) Total Burden Hours is used in Table 6-B of this ICR for estimating the burden & costs related to data generation paperwork activities.

7.310627% Effective Inflation Rate Calculated from Bureau of Labor Statistics, CPI Inflation Calculator, 2009$ => 2012$, as of January 10, 2013

As of 4/3/2013 Renewal http://www.bls.gov/data/inflation_calculator.htm 

### Calculations for Paperwork Burden and Costs for Data Generation Activities

Estimated Cost ($) Estimated Burden (Hours)

(a-1) Estimated costs for these assays are from Table 8 in the EPA report entitled: "Laboratory Testing of Chemicals for Endocrine Disruption Potential - Analysis of Market Factors" (October 14, 2009) (Ref. 7). Shared costs

in columns 3 & 4 of that table were divided by the tests included in the group, which include analytical chemistry where appropriate.

(a-2) The estimated costs for these assays were adjusted using an inflation factor to convert 2009$ to 2012$. Specifically, the estimated cost in (a-1) was entered into the online inflation calculator provided by US

Department of Labor, Bureau of Labor and Statistics at