# Innovative Remediation

**Technology for Soil & Groundwater**  **Contamination at Sites Containing MTBE and other** 

**Pollutants Global**  **BioSciences ,**  **LLC**

### • **Metabolism -**  **bacterial cells**  **receive energy from**  **the degradation**  **processes (i.e.**  **of butane and other petroleum**  **hy drocarbons)** 

**- good for petroleum**  **degradation**

• **Cometabolism -**  **bacteria grow**  **ing on a particular**  **food source (butane)**  **produce enzy mes that**  **degrade a second substrate**  **(MTBE) (bacteria**  **do not gain energy from the**  **cometabolic process)**  

# Butane Biological Pr**ocesses**

**Good for MTBE and** 

## chlorinated **solvents degradation** 

# Pr**ove n Ef** **fe ctiv** **e Re**  **mo val of...** 

• **MTBE**

• **Chlorinated Solvents**

• **Pesticides**

• **PCBs**

• **Petroleum Hy drocarbons**  • **Gasoline**

• **PA Hs** 

• **1,4 -**  **Dioxane**

• **Aromatics**

•**And a variety of other pollutants**

# GBI: En**vir onmental R** **emedia tion** 

• **Simpler and more efficient remediation**  **of soil and groundw**  **ater**

– **Proprietary**

**butane technology**

– **Stimulates indigenous soil and groundw**  **ater bacteria** 

– **Enzy mes produced degrade contaminants**  **in -** **situ**

– **No soil excavation or disposal required**

– **Proven track -**  **record; technology**  **of choice in several**  **states**

– **Cleans multiple common contaminants** 

# Butane Injection **System**

**Skid Size: 4 x 4 x 6 (f**  **eet)**

# Butane Injection **System**

**Skid built into shed to pr ov**  **ide comf**  **or table w** **or k space f**  **or on -**  **site wo**  **rk er** **s**

# Portable Injection Hardware for Small Sites

• **Portability or mobility allow s**  **hot**   ** spot treatment and butane** **treatment at small release Sites.**

• **Flexibility permits soil and groundw**  **ater treatment in tightly settled or built**  **- up**  **areas by**  **utilizing existing monitoring w**  **ells as butane injection w**  **ells.**

• **For hot -** **spot treatment, the mobile butane treatment system**  **wi ll**  **significantly low er**  **capital costs for injection w**  **ell installation.** 

• **Dramatically low**  **ers remediation costs for small sites.** 

# Deliv**er y**  **System Flo** **w Dia gra**  **m**

**Note: Activa** 

**ted carbon has been**  **re mo**  **ve d**  **fr om pr**  **ocess str** 

**eam –**  **lar**

**ge cost sa**  **vings to c** 

**lient**

# Pr**ocess Cur re**  **ntl y**  **Accepted by ...** 

• **US Environmental Protection Agency**  **(for Superfund**  **Site remediation)**

• **Massachusetts Department**  **of Environmental Protection**

• **Florida Department of Environmental Protection** 

• **Pennsy lvania**  **Department of Environmental Protection** 

• **Iow a**  **Department of Natural**  **Resources Acceptance**  **in other states highly likely (never**  **rejected)**

# Butane Saf **ety**

• **Butane distribution system mixes butane w**  **ith air**  **prior to injecting the mix into the soil**

• **Concentrations generated in the subsurface w ith**  **the butane injector are less than 0.01% of the LEL** **for butane in air**

• **Ty pically**  **butane gas is used**  **in shav ing cream**  **cans and also**  **used in food products**

• **Local fire departments typically**  **require a permit to store Butane** 

• **Excess butane (C 4**  **H 10**  **) in soil/groundw ater decomposes into**  **CO 2**  **, H 2**  **O, and other inert species in 2 -**  **3 days**

• **Fugitiv e**  **emissions are never**  **generated as a result of the process** 

# • **MTBE**

• **Chlorinated Solvents**  

# Case Studies

## Mobil Gasoline Sta **tion in W inthr** **op, MA**

## Mobil Gasoline Sta **tion in W inthr** **op, MA** Ti me Period Start - up + 7 Months Parameters VPH (ug/L) VW- 1 VW- 1 Methyl tert - butyl ether (MBTE) 16,000 103 Benzene <300 5.2 To luene <300 <5.0 Ethylbenzene <300 5.4 Xylenes <300 40.9 Naphthalene <300 27.7 C 5 - C 8 Aliphatic Hydrocarbons <500 <40 C 9 - C 12 Aliphatic Hydrocarbons 930 <15 C 9 - C 10 Aromatic Hydrocarbons <500 303 System start- up date 12/12/2001 System operation was terminated on November 10, 2003; Site is undergoing post-remedial monitoring

## Citgo **Gasoline Sta tion in Salem, MA** 

# Citgo Gasoline **Sta tion**  **in Salem, MA**  Ti me Period Start - up + 6 Months Parameters VPH (ug/L) MW- 3 MW- 3 Methyl tert - butyl ether (MBTE) 206,000 4,090 Benzene 787 <5.0 To luene 21 1 <5.0 Ethylbenzene 66.3 <5.0 Xylenes 322 <5.0 Naphthalene 58 <20 C 5 - C 8 Aliphatic Hydrocarbons <8,000 <40 C 9 - C 12 Aliphatic Hydrocarbons 897 <15 C 9 - C 10 Aromatic Hydrocarbons 935 <55 System start- up date 12/2/2002;

## DPW Maintenance F **acility in**

**Ke ystone Heights, Florida** 

# DPW Maintenance **Fa cility**  **in K eystone** 

**Heights, Florida (**  **Silty -**  **Clay Soils)** 

Ti me Period Start - up + 4 Months Parameters VPH (ug/L) MW- 2 MW- 2 Methyl tert - butyl ether (MBTE) 4.0 <1.0 Benzene 3.0 <1.0 To luene 83 <1.0 Ethylbenzene 387 <1.0 Xylenes 1,190 <1.0 Naphthalene 92 <1.0 System start- up date 4/4/2002; System operation was terminated on 3/4/2003; First Site closed in Florida using butane technology

## Nantuc **ke t Steamship Authority**  **Site in F airha** **ve n, MA** 

# Nantuc **ke t**  **Steamship Authority**  **Site in**  **Fa irha** **ve n,**  **MA**

System start- up date December, 2001; System operation was terminated in June 2002

Ti me Period Start - up + 6 Months Parameters VPH and EPH (ug/L) MW- 4 MW- 4 C 5 - C 8 Aliphatic Hydrocarbons 54 <40 C 9 - C 12 Aliphatic Hydrocarbons 830 <15 C 9 - C 10 Aromatic Hydrocarbons 1,200 583 C 9 - C 18 Aliphatic Hydrocarbons 19,000 <100 C 19 - C 36 Aliphatic Hydrocarbons 2,400 <100 C 11 - C 22 Aromatic Hydrocarbons 30,000 <100

## Sunoco **Gasoline Sta tion in Canton, MA** 

# Sunoco **Gasoline Sta**  **tion in Canton, MA**  Ti me Period Start - up +1 1 Months Parameters VPH (ug/L) MW- 3 MW- 3 Methyl tert - butyl ether (MBTE) 370 52 Benzene 850 <5.0 To luene 830 <5.0 Ethylbenzene 380 <5.0 Xylenes 2,000 <5.0 Naphthalene 11 0 <10 C 5 - C 8 Aliphatic Hydrocarbons 1,900 <50 C 9 - C 12 Aliphatic Hydrocarbons 660 <50 C 9 - C 10 Aromatic Hydrocarbons 1,700 <50 System start- up date 1 1/10/2000; System operation was terminated on 10/29/2002; Site closed after one year of post - remedial monitoring

## Butane R**emedia tion of** **T CA/T** **CE in Sioux City** 

**, IA** 

# Butane **Remediation of TCA/TCE**

**Project in Sioux City** 

**, Iowa for General Motors Corporation**  Sampling Date 2/14/2001 4/1 1/2001 Parameters Before After VOCs (ug/L) AC - 155 1,1,1 - Tr ichloroethane 7,160 0.7 1,1,2 - Tr ichloroethane 0 0 1,1 - Dichloroethane 5,170 1.6 1,1 - Dichloroethene 1,400 0 1,2 - Dichloroethane 0 0 cis - 1,2 - Dichloroethene 5,720 3.5 Te trachloroethene 0 0 Tr ichloroethene 1,400 0 Vi nyl Chloride 0 0

## • **Hardw are design is rugged, simple, and safety** 

**certified (UL Listed)** 

• **Hardw are has approval from State Fire**  **Marshals**

• **Injection system**  **complies w ith**  **federal, state and local law** **s and regulations pertaining to**  **compressed gas delivery a**  **nd storage**

• **Single injection panel (6**  **- 14 w**  **ells) covers ~ 1**  **- 2**  **acre area** 

# Injection Hardware is Simple, Safe **And Code Compliant**

## • **Simple, rapid, effective for a w ide**  **variety o**  **f**

**environmental pollutants**

• **Cost -**  **effective and readily adaptable to large or**  **small clean -**  **ups**

• **No soil excavation or disposal necessary (** **very minimal**  **surface disruption)**

• **Subsurface bacterial populations return to**  **background levels after process is stopped**

• **Proven track -**  **record, acceptance grow ing** 

• **Enormous market opportunity** 

# Butane Biostimulation Summary

# Th **ank Y ou**  **!**

• www .GlobalBioSciencesllc.org