ii 

TABLE OF CONTENTS

I. PRELIMINARY WORK PLAN ................................................................................................................................ 4

A. Overview .......................................................................................................................................................... 4

B. Statutory and Regulatory Authority .................................................................................................................. 5

C. Background ...................................................................................................................................................... 5

1. Summary of the Active Ingredient ............................................................................................................... 5

2. Mode of Action ............................................................................................................................................. 5

3. Registered Products .................................................................................................................................... 6

4. Application Rates and Methods ................................................................................................................... 6

5. Use Pattern .................................................................................................................................................. 7

6. Tolerance Actions ........................................................................................................................................ 8

D. Anticipated Data Needs and Risk Assessments .............................................................................................. 8

1. Product Analysis: 40 CFR 158.2120 .......................................................................................................... 8

2. Toxicology: 40 CFR 158.2140 .................................................................................................................... 9

3. Nontarget Organisms and Environmental Fate: 40 CFR 158.2150 ......................................................... 10

E. Risk to Threatened and Endangered Species ............................................................................................... 10

F. Endocrine Effects ........................................................................................................................................... 10

G. Incidents ......................................................................................................................................................... 11

H. Summary of Anticipated Data Needs ............................................................................................................. 11

I. Timeline ........................................................................................................................................................... 12

J. Guidance for Commenters .............................................................................................................................. 13

1. Environmental Justice ............................................................................................................................... 13

2. Water Quality ............................................................................................................................................. 13

3. Trade Irritants ............................................................................................................................................ 13

4. Additional Information ................................................................................................................................ 14

K. Next Steps ...................................................................................................................................................... 14

II. FACT SUMMARY FOR CASE 6088 .................................................................................................................. 14

III. BIBLIOGRAPHY ................................................................................................................................................ 15

A. Studies Supporting Registration Review of Streptomyces lydicus WYEC 108 .............................................. 15

B. U.S. Environmental Protection Agency Risk Assessment Memoranda ......................................................... 17

C. Other References ........................................................................................................................................... 19

TABLES 

Table 1 Product Analysis Data Requirements and How They Are Met .............................................................. 8

Table 2 Human Health Toxicology Data Requirements and How They Were Met ............................................ 9

Table 3 Ecological Toxicity Data Requirements and How They Were Met ...................................................... 10

Table 4 Anticipated Data Needs for Case 6088—Streptomyces lydicus WYEC 108 ...................................... 11

APPENDICES 

Appendix I Regulatory History Pertaining to the Registration of Products Containing 

 S. lydicus WYEC 108 as an Active Ingredient 

Appendix II Plant Diseases Controlled by S. lydicus WYEC 108 

Appendix III Use Sites for Products Containing S. lydicus WYEC 108 as an Active Ingredient 

 
iii 

ACRONYMS AND ABBREVIATIONS

CFR Code of Federal Regulations 

CSF Confidential Statement of Formulation 

DCI Data Call-in 

EDSP Endocrine Disruptor Screening Program 

EPA U.S. Environmental Protection Agency, or the Agency

FFDCA Federal Food, Drug, and Cosmetic Act 

FIFRA Federal Insecticide, Fungicide, and Rodenticide Act 

FQPA Food Quality Protection Act 

FR Federal Register 

FWP Final Work Plan 

IDS Incident Data System 

MPCA Microbial Pest Control Agent 

MRID Master Record Identification Number 

MRLs Maximum Residue Limits 

OPP Office of Pesticide Programs 

OPPIN Office of Pesticide Programs Information 

PWP Preliminary Work Plan 

S. lydicus Streptomyces lydicus 

TMDLs Total Maximum Daily Loads 
I. PRELIMINARY WORK PLAN

A. Overview

Case Name: Streptomyces lydicus WYEC 108 

Case No: 6088 

PC Code: 006327

Docket: EPA-HQ-OPP-2014-0608 

Registrant: Novozymes BioAg 

 The docket for Streptomyces lydicus WYEC 108 is now open, initiating the first public comment 

period for Registration Review Case 6088. This pesticide active ingredient is a bacterium that is 

registered for use to control a number of soil- and foliar-borne plant diseases generally caused by fungi. 

 This Preliminary Work Plan (PWP) document explains what the U.S. Environmental Protection 

Agency’s (EPA or the Agency) Office of Pesticide Programs (OPP) knows about Streptomyces lydicus

WYEC 108, highlighting anticipated data and assessment needs, identifying types of information that 

would be especially useful to EPA in conducting the review, and providing an anticipated timeline for 

completing review of this active ingredient. 

 The Registration Review process was designed to include a public participation component to 

solicit input from interested stakeholders. By sharing this information in the docket, EPA intends to 

inform the public of what it knows about Streptomyces lydicus WYEC 108 and what types of new data 

or other information would be helpful for the Agency to receive as it moves toward a decision on this 

active ingredient. EPA encourages all interested stakeholders to review the PWP and to provide 

comments and additional information that will help EPA’s decision-making process for this microbial 

pesticide. In addition to general areas on which persons may wish to comment, there are some areas 

identified in the PWP about which EPA specifically seeks comments and information. Interested 

stakeholders could include the following: environmental nonprofit or interest groups; pesticide 

manufacturers; agricultural labor or commodity groups; commercial, institutional, residential, and other 

users of pesticides; or the public at large. 

 The PWP begins by discussing the statutory and regulatory authority for Registration Review. 

Next, it provides background on Streptomyces lydicus WYEC 108 including a description of the modes 

of action; currently registered pesticide products; application rates and methods; use sites; and 

tolerance exemption information. Then, it lists the anticipated data needs and risk assessments and a 

projected Registration Review timeline for Streptomyces lydicus WYEC 108. Finally, the PWP 

describes guidance for commenters; explains the next steps EPA will take for this Registration Review; 

summarizes background information for this Registration Review; and lists supporting studies, risk 

assessment memoranda, and other documents. 

 Further information about this case, including background and supporting documents, is 

available at http://www.regulations.gov (under “SEARCH for: Rules, Comments, Adjudications or 

Supporting Documents,” enter the docket identification number, which is indicated above). 

 

 
 5 

B. Statutory and Regulatory Authority

 The Food Quality Protection Act (FQPA) of 1996 mandated a Registration Review program. All 

pesticides distributed or sold in the United States must generally be registered by EPA, based on 

scientific data showing that they will not cause unreasonable risks to human health or the environment 

when used as directed on the product labeling. The Registration Review program is intended to make 

sure that, as the ability to assess risk evolves and as policies and practices change, all registered 

pesticides continue to meet the statutory standard of no unreasonable adverse effects to human health 

or the environment. Changes in science, public policy, and pesticide use practices will occur over time. 

Through the Registration Review program, EPA periodically reevaluates pesticides to make sure that, 

as change occurs, products in the marketplace can continue to be used safely. Information on this 

program is provided on EPA’s website. 

 

 EPA is implementing the Registration Review program pursuant to Federal Insecticide, 

Fungicide, and Rodenticide Act (FIFRA) section 3(g) and will review each registered pesticide every 

15 years to determine whether it continues to meet the FIFRA standard for registration. The 

regulations governing Registration Review begin at 40 CFR 155.40. EPA will consider benefits 

information and data as required by FIFRA. The public phase of Registration Review begins when the 

initial docket is opened for each case. The docket is EPA’s opportunity to state what it knows about the 

pesticide and what additional risk analyses and data or information it believes are needed to make a 

Registration Review decision. After reviewing and responding to comments and data received in the 

docket during this initial comment period, EPA will develop a Final Work Plan (FWP) and anticipated 

schedule for the Registration Review of Streptomyces lydicus WYEC 108. 

C. Background

1. Summary of the Active Ingredient 

Streptomyces lydicus is a naturally-occurring bacterium that is commonly found in soil 

environments. Its taxonomic classification is (NLM 2014): 

Kingdom: Bacteria 

Phylum: Actinobacteria 

Class: Actinobacteria 

Order: Actinomycetales 

Family: Streptomycetaceae 

Genus: Streptomyces

Species: lydicus

Streptomyces lydicus WYEC 108 was first isolated by Crawford et al. (1993) from soil surrounding 

linseed plants found in a grassland sometimes grazed by sheep at Hastings Hill, South Downs, West 

Sussex, England. 

 2. Mode of Action 

Streptomyces lydicus colonizes growing root tips of plants and acts as a mycoparasite of fungal 

root pathogens which helps protect the plants. Other possible mechanisms include the production and 

excretion of antifungal metabolites (e.g., antibiotics and/or low molecular weight antifungal compounds 

or lytic enzymes like chitinase) after colonization. (U.S. EPA 2004d) Tokala et al. (2002) points out that 

the strain WYEC 108 is a “plant growth-promoting bacterium in the absence of fungal pathogen 

challenge. This may be due to the ability of strain WYEC 108 to produce hydroxamate-type 

siderophores and/or other plant growth-promoting metabolites in the rhizosphere.” 
 6 

3. Registered Products 

 Novozymes BioAg is the sole registrant for pesticide products containing Streptomyces lydicus

WYEC 108 as an active ingredient. EPA registered the first product containing this active ingredient in 

2004; today three such products are registered: 

EPA 

Reg. No

Product 

Name 

% Active 

Ingredient First Registered 

73314-1 Actinovate® Soluble 

Biological Fungicide 0.0371 May 24, 2004 

73314-2 Actino-Iron® 

Biological Fungicide 0.013 January 12, 2005 

73314-4 Actinovate® STP 

Fungicide 0.33 December 19, 2007 

 

Appendix I provides a detailed regulatory history for the registration of these products. 

 4. Application Rates and Methods 

 Pesticide products containing Streptomyces lydicus WYEC 108 may be applied to soil (as a 

drench or mixed with soil); foliage (using backpack sprayer, aerially, or via ground equipment); seed 

(using commercial seed treatment equipment); roots being transplanted (cuttings or bare roots); 

ornamental bulbs (as a soak, dust, or drench); and turf (with a spreader). Provided below is a summary 

of the application rates for the three labels. 

. 

73314-1: Actinovate® Soluble Biological Fungicide 

Greenhouse: nursery, ornamentals, vegetables & herbs 

Soil Application Foliar Application 

4 to 6 oz in 100 gal water (drench) 6 to 12 oz in 50 to 100 gal water 

1.5 to 4 oz per yard soil (blending) 

Transplant Dip

6 to 18 oz in 50 gal water 

Ornamental Bulbs

Soak Dust Drench 

6 to 18 oz 

in 100 gal water 

2 to 6 oz 

per 100 lb bulbs 

6 to 12 oz 

in 10 to 200 gal water 

Agricultural Production 

Soil Treatment Seed Treatment Foliar Application 

1 to 12 oz in 

10 to 200 gal 

water/Acre soil 

2 to 18 lb/A seed 1 to 12 oz in 

10 to 150 gal 

water/Acre 

Turf 

18 to 54 oz/Acre 

 
 7 

73314-2: Actino-Iron® Biological Fungicide (soil only) 

Greenhouses and Nurseries Potting Soil Mix 

5 lb/yd3 of planting media 

Nursery Beds 

2 to 8 lb/1,000 ft2

Top Dressing 

3 oz/ft3 planting media 

Turf 

4 to 9 oz/1000 ft2 grass 

Outdoor Field and Ornamental 

10 to 15 lb/A 

 

73314-4: Actinovate® STP Fungicide (seed treatment only)

Seed Coating 

0.0088 to 0.28 oz/lb seed 

 5. Use Pattern 

 Products containing Streptomyces lydicus WYEC 108 as an active ingredient are registered for 

use to treat a number of root and foliar plant diseases generally caused by fungi and molds (e.g., 

Alternaria, Botrytis, Erysiphe, Sclerotinia, Phytophthora), and in a few cases, by bacteria (e.g., Erwinia, 

Xanthomonas), or nematodes (Belonolaimus longicaudatus, Tylenchulus semipenetrans). Appendix II 

provides a comprehensive list of the diseases against which products containing Streptomyces lydicus

WYEC 108 are registered for use. 

 The use pattern for Streptomyces lydicus WYEC 108 containing products is quite broad and 

includes food and nonfood agricultural and residential uses. Food uses range from seed treatments to 

foliar and soil application to a wide-array of food commodities including animal feed such as grasses 

and hay. Specific food crops, which may be greenhouse- or field-grown, include: cucurbits (e.g., 

melons); leafy vegetables and herbs (e.g., broccoli, cabbage, oregano); root and bulb vegetables (e.g., 

beets, garlic, potatoes); tree fruits (e.g., apples, cherries, citrus); nuts and berries (almonds, 

blueberries, pistachio nuts); grains and legumes (e.g., barley, millet, wheat); and other food crops such 

as bananas and grapes. Nonfood uses include application to an array of ornamental plants grown in 

the field, nurseries, and greenhouses. These include: turf (athletic fields, golf courses); grasses (St. 

Augustine, zoysiagrass); and other ornamental plants (bulbs, flowering plants, perennials). Provided 

in Appendix III is a complete list of use sites for products containing Streptomyces lydicus WYEC 108 

as an active ingredient. 

 
 8 

6. Tolerance Actions 

 An exemption from the requirement of tolerance was established through a Final Rule published 

on June 3, 2004 (69 FR 31301); it reads: 

§ 180.1253 Streptomyces lydicus WYEC 108; Exemption from the Requirement of a Tolerance. 

An exemption from the requirement of a tolerance is established for residues of the microbial 

pesticide Streptomyces lydicus WYEC 108 when used in or on all agricultural commodities when 

applied/used in accordance with label directions. 

No changes have been made to this exemption since its establishment in 2004. 

 D. Anticipated Data Needs and Risk Assessments

 Provided below are tables identifying the current data requirements for microbial pesticides as 

set forth in 40 CFR part 158, subpart V; they are arrayed by scientific discipline: Table 1—Product 

Analysis, Table 2—Human Health Toxicology, and Table 3—Ecotoxicity. Each table lists the respective 

data requirements and shows how the requirement has been met (or not met). The term “Guideline” 

refers to the Agency test guidelines for microbial pesticides; these can be found under the 700 Series 

at: http://nepis.epa.gov/EPA/html/pubnumindex.html. 

EPA has identified several physical and chemical property data gaps (under Product Analysis, 

Table 1) which are anticipated data needs for this registration review. All other data requirements have 

been fulfilled. 

 1. Product Analysis: 40 CFR 158.2120 

Table 1 Product Analysis Data Requirements and How They Are Met 

Guideline Data 

Requirement 

How the Requirement Is Met (MRID; Review)

EPA Reg. No 73314-1

Actinovate® Soluble

EPA Reg. No 73314-2 

Actino-Iron® 

EPA Reg. No 73314-4

Actinovate® STP

Product Chemistry and Composition

885.1100 Product identity 451413-01; 

U.S. EPA 2001b, 2007c 

885.1200 Manufacturing processi 451413-01; 

U.S. EPA 2001b 

462980-01; 

U.S. EPA 2004g 

472205-01; 

U.S. EPA 2007c 

885.1250 

Deposition of a sample in a 

nationally recognized 

culture collection. 

451413-01; 

U.S. EPA 2001b

885.1300 Discussion of formation of 

unintentional ingredients 

451413-01; 

 U.S. EPA 2001b 

462980-01; 

U.S. EPA 2004g 

471876-01; 

U.S. EPA 2007c 

Analysis and Certified Limits 

885.1400 Analysis of samples 460572-01; 

U.S. EPA 2004b 

464234-01; 

U.S. EPA 2005a, b 

472205-01; 

U.S. EPA 2007c 

885.1500 Certification of limits information provided on: 

June 26, 2013 CSFii

information provided on 

November 1, 2013 CSF 

information provided on 

December 7, 2007 CSF 

Physical and Chemical Characteristics

830.6302 Color DATA GAP DATA GAP 471876-01; 

 U.S. EPA 2007c 

830.6303 Physical state 451413-01; 

U.S. EPA 2001b, 2004b 

462980-01; 

U.S. EPA 2004g 

471876-01; 

U.S. EPA 2007c 
 9 

Guideline Data 

Requirement 

How the Requirement Is Met (MRID; Review)

EPA Reg. No 73314-1

Actinovate® Soluble

EPA Reg. No 73314-2 

Actino-Iron® 

EPA Reg. No 73314-4

Actinovate® STP

830.6304 Odor DATA GAP DATA GAP 471876-01; 

U.S. EPA 2007c 

830.6313 

Stability to normal and 

elevated temperatures, 

metals, and metal ions 

DATA GAP DATA GAP DATA GAP

830.6317 Storage stability 451413-01; 

U.S. EPA 2001b 

462980-01; 

U.S. EPA 2004g 

471876-01; 

U.S. EPA 2007c, d 

830.6319 Miscibility NAiii: only required for emulsifiable liquid forms of 

microbial pesticides; these products are solids. 

830.6320 Corrosion characteristics DATA GAP 464234-01; 

U.S. EPA 2005a DATA GAP

830.7000 pH 460572-01; 

U.S. EPA 2001b, 2004b 

462980-01; 

U.S. EPA 2004g DATA GAP

830.7100 Viscosity NAiv: Only required for liquid forms of 

microbial pesticides; these products are solids. 

830.7300 Density DATA GAP DATA GAP DATA GAP

i

Products containing Streptomyces lydicus WYEC 108 as an active ingredient are manufactured using an ‘integrated process,’ which 

means that there is no separate manufacturing-use product. iiCSF=Confidential Statement of Formulation. iii40 CFR 158.2120(d)3 

iv40 CFR 158.2120(d)5

 2. Toxicology: 40 CFR 158.2140 

Table 2 Human Health Toxicology Data Requirements and How They Were Met 

Guideline Data 

Requirement

How the Requirement Is Met (MRID; Review) 

EPA Reg. No 73314-1 

Actinovate® Soluble 

EPA Reg. No 73314-2 

Actino-Iron® 

EPA Reg. No 73314-4 

Actinovate® STP 

885.3050 Acute oral toxicity/ 

pathogenicity 

Satisfied with scientific rationale (TSG 2002); 

U.S. EPA 2003a, 2004d 

885.3150 Acute pulmonary 

toxicity/pathogenicity 451117-02; U.S. EPA 2001b, 

885.3200 Acute injection toxicity/ 

pathogenicity (i.v.) 451117-03; U.S. EPA 2001b 

885.3400 Hypersensitivity 

incidents 

Data Waived: Registrant reported (MRID 470434-02) that no such incidents have 

occurred for any of their products containing S. lydicus WYEC 108. Further, they stated

that should any incidents occur, they will be reported under FIFRA section 6(a)(2). 

885.3500 Cell culture NAi: only required for virus; Streptomyces lydicus is a bacterium.

870.1100 Acute oral toxicity 451117-01; 

U.S. EPA 2001b, 2003b 

462980-02; 

U.S. EPA 2004g 

451117-01; 

U.S. EPA 2007d 

870.1200 Acute dermal toxicity 451070- 00; 

U.S. EPA 2004c 

462980-03; 

U.S. EPA 2004g 

462980-03; 

U.S. EPA 2007d 

870.1300 Acute inhalation 

toxicity 

451117-04; 

U.S. EPA 2003b 

462980-04; 

U.S. EPA 2004g 

462980-04; 

U.S. EPA 2007d 

870.2400 Acute eye irritation 451117-04; 

U.S. EPA 2001b, 2003b 

462980-05; 

U.S. EPA 2004g 

451117-04; 

U.S. EPA 2007d 

870.2500 Primary dermal 

irritation 

451117-05; U.S. EPA 

2001b, 2003b, 2004c 

462980-06; 

U.S. EPA 2004g 

451117-05; 

U.S. EPA 2007d 

i

40 CFR 158.2140(d)4
 10 

 3. Nontarget Organisms and Environmental Fate: 40 CFR 158.2150 

Table 3 Ecological Toxicity Data Requirements and How They Were Met 

Guideline Data Requirement How the Requirement Is Met 

(MRID; Review)

Tier I

885.4050 Avian oral toxicity 468739-01; U.S. EPA 2006a 

885.4100 Avian inhalation toxicity/pathogenicity 470434-02; U.S. EPA 2003b

885.4150 Wild mammal toxicity/pathogenicity 470434-02; U.S. EPA 2003b

885.4200 Freshwater fish toxicity/pathogenicity 451117-06; U.S. EPA 2001a 

885.4240 Freshwater invertebrate toxicity/pathogenicity 468739-02; U.S. EPA 2006a 

885.4280 Estuarine/Marine fish testing 

Estuarine and marine invertebrate testing 

470434-02; U.S. EPA 2003b 

885.4300 Nontarget plant testing 468739-03; U.S. EPA 2006a 

885.4340 Nontarget insect testing 470434-02; U.S. EPA 2003b 

885.4380 Honey bee testing 468739-04; U.S. EPA 2006a 

E. Risk to Threatened and Endangered Species

 The Agency has not prepared a risk assessment that supports a complete endangered species 

determination for Streptomyces lydicus WYEC 108. During Registration Review, EPA plans on 

conducting an ecological risk assessment to determine whether use of Streptomyces lydicus WYEC 

108 will have “no effect” or “may affect” federally listed threatened or endangered species (listed 

species) and/or their designated critical habitat. When an assessment concludes that a pesticide’s use 

“may affect” a listed species and/or its designated critical habitat, EPA will consult with the U.S. Fish 

and Wildlife Service and/or National Marine Fisheries Service (the Services), as appropriate.

 F. Endocrine Effects

 As required by the Administrator under the Federal Food, Drug, and Cosmetic Act (FFDCA) 

section 408(p), EPA has developed the Endocrine Disruptor Screening Program (EDSP) and has 

begun to implement the screening program that is to be used to test all pesticides to determine whether 

certain substances (including pesticide active and other ingredients) may have an effect in humans or 

wildlife similar to an effect produced by a “naturally occurring estrogen, or other such endocrine effects 

as the Administrator may designate.” 

 FFDCA section 408(p)(4) authorizes the Administrator, by order, to exempt from the 

requirements of the Estrogenic Substances Screening Program a biological substance or other 

substance if a determination is made that the substance is not anticipated to produce any effect in 

humans similar to an effect produced by a naturally occurring estrogenic substance. 

 Between October 2009 and February 2010, EPA issued test orders/data call-ins (DCI’s) for the 

first group of 67 chemicals, which contains 58 pesticide active ingredients and nine inert ingredients. 

Streptomyces lydicus WYEC 108 is not among the group of 58 pesticide active ingredients on the initial 

list to be screened under the EDSP. 
 11 

 EPA believes that Streptomyces lydicus WYEC 108, the active ingredient in this Registration 

Review case, likely would not produce any effect in humans similar to an effect produced by a naturally 

occurring estrogenic substance. As such, pursuant to Section 408(p)(4), EPA will determine in the 

future whether it can exempt Streptomyces lydicus WYEC 108 from the requirements of section 408(p) 

of EDSP. If EPA determines to exempt this substance from the EDSP, then it will issue an order. 

 For further information on the status of the EDSP, the policies and procedures, the list of 67 

chemicals, future lists, the test guidelines and the Tier 1 screening battery, please visit our website at 

http://www.epa.gov/endo/. 

G. Incidents 

Over the period June 1992 to August 2014, no adverse human, domestic animal, or 

environmental incidents involving exposure to Streptomyces lydicus WYEC 108 as an active ingredient 

in a pesticide formulation have been reported to the Office of Pesticide Programs’ Incident Data System 

(U.S. EPA 2014). The Agency will consider any incident data or comments submitted to this docket in 

response to this PWP. 

 

 H. Summary of Anticipated Data Needs 

 The Agency expects to issue a DCI for the following product analysis data gaps. 

Table 4 Anticipated Data Needs for Case 6088—Streptomyces lydicus WYEC 108 

Product Analysis 

(40 CFR 158.2120)

Human Health Toxicity

(40 CFR 158.2140) 

Ecological Toxicity 

(40 CFR 158.2150) 

Guideline Requirement 

Timeframe 

(months from 

receipt of DCI)

No data gaps. No data gaps. 

830.6302 Color 8 months 

830.6304 Odor 8 months

830.6313 

Stability to normal and 

elevated temperatures, 

metals, and metal ions 

8 months

830.6320 Corrosion characteristics 8 months

830.7000 pH 8 months

830.7300 Density 8 months

 
 12 

I. Timeline

 The projected timeline for Case 6088 (Streptomyces lydicus WYEC 108) is as follows: 

Activity Month Year 

(estimated) 

Docket Opening

Open Docket and 60-Day Public Comment Period September 2014 

Close Public Comment Period November 2014 

Case Development

Issue Final Work Plani May 2015 

Issue Data Call-in May 2016 

Data Submission January 2017 

Open 30-Day Public Comment Period 

 for Draft Risk Assessments June 2017 

Close Public Comment Period July 2017 

Registration Review Decision

Open 60-Day Public Comment Period 

 for Proposed Registration Review Decision November 2017 

Close Public Comment Period January 2018 

Final Decision February 2018 

IESTIMATED TOTAL (YEARS): 4.5 

i

This schedule is subject to revision should unforeseen issues arise during the 

Registration Review process. If an issue arises (e.g., failure to acquire an EDSP 

exemption), an amended FWP may be issued at that time. That FWP will identify any 

newly anticipated data needs and set forth a new timeline. 

 
 13 

J. Guidance for Commenters

 

 The public is invited to comment on the PWP and rationale. The areas below highlight topics of 

special interest to EPA where comments, information submissions, or reference to sources of additional 

information could be of particular use. EPA will consider all comments, as well as any additional 

information or data provided in a timely manner, prior to issuing an FWP for this case. 

1. Environmental Justice 

 EPA seeks to achieve environmental justice, the fair treatment and meaningful involvement of 

all people, regardless of race, color, national origin, or income, in the development, implementation, 

and enforcement of environmental laws, regulations, and policies. To help address potential 

environmental justice issues, EPA seeks information on any groups or segments of the population who, 

as a result of their location, cultural practices, or other factors, may have atypical, unusually high 

exposure to Streptomyces lydicus WYEC 108 compared to the general population. Please comment if 

you are aware of any subpopulations that may have atypical, unusually high exposure compared to the 

general population. 

 2. Water Quality 

 

Streptomyces lydicus WYEC 108 is not identified as a cause of impairment for any water bodies 

listed as impaired under section 303(d) of the Clean Water Act. In addition, EPA has not developed 

Total Maximum Daily Loads (TMDLs) for Streptomyces lydicus WYEC 108. More information on 

impaired water bodies and TMDLs can be found at EPA’s website. EPA invites submission of water 

quality data for this pesticide. To the extent possible, data should conform to the quality standards in 

Appendix A of the Office of Pesticide Programs’ Standard Operating Procedure: Inclusion of Impaired 

Water Body and Other Water Quality Data in OPP’s Registration Review Risk Assessment and 

Management Process to ensure they can be used quantitatively or qualitatively in pesticide risk 

assessments. 

 3. Trade Irritants 

 Through the Registration Review process, EPA intends to solicit information on trade irritants 

and, to the extent feasible, take steps toward facilitating irritant resolution. The Agency will work to 

harmonize tolerance and international Maximum Residue Limits (MRLs) and may modify tolerance 

levels to do so, when possible. Growers and other stakeholders are asked to comment on any trade 

irritant issues resulting from lack of MRLs or disparities between U.S. tolerances and MRLs in key 

export markets, providing as much specificity as possible regarding the nature of the concern. 

 The Agency has exempted Streptomyces lydicus WYEC 108 from the requirement of a 

tolerance. Moreover, there are no known MRLs for Streptomyces lydicus WYEC 108. Therefore, EPA 

does not anticipate that current uses of this pesticide active ingredient will pose concerns as trade 

irritants. 

 
 14 

 4. Additional Information 

 Stakeholders also are asked to provide information and data that will assist EPA in refining the 

risk assessments. The Agency is interested in obtaining the following information on Streptomyces 

lydicus WYEC 108: 

K. Next Steps

 

 After the 60-day comment period closes, EPA will review and respond to any comments 

received in a timely manner and then issue an FWP for Case 6088. 

II. FACT SUMMARY FOR CASE 6088 

Active Ingredient 

(PC Code) 

First 

Registered 

Registered 

Pesticide 

Productsi

Publicly Available 

EPA Science and 

Regulatory 

Documents 

Additional Risk Assessment Needed? 

Product 

Chemistry 

Human 

Health Ecological 

Streptomyces 

lydicus WYEC 108 

(006327) 

May 24, 2004 Three end-use 

productsii

BRAD 

 (U.S. EPA 2004d) Yes No Yes 

i

See Appendix II and III for additional information (i.e., use sites and diseases controlled) on the currently 

registered products. Labels for these products can be found in the Pesticide Product Label System at: 

http://iaspub.epa.gov/apex/pesticides/f?p=PPLS:1 

iiNote: there is no manufacturing product because the products are made using integrated systems. 

 

 Confirmation on the following label information: 

 - Formulations 

 - Application sites 

 - Application methods and equipment 

 - Maximum application rates 

 - Frequency of application, application intervals, 

and maximum number of applications 

 - Geographic limitations on use 

 Use or potential use distribution (e.g., acreage and 

geographical distribution of relevant use sites) 

 Median and 90th percentile reported use rates (lbs 

active ingredient/1,000 square feet) from usage 

data—national, state, and county 

 Application timing (date of first application and 

application intervals)—national, state, and county 

 Usage/use information for nonagricultural uses 

 Typical application interval (days) 

 State or local use restrictions 

 Monitoring data 
 15 

III. BIBLIOGRAPHY

A. Studies Supporting Registration Review of Streptomyces lydicus WYEC 108 

MRID 451070-01 Roberts, A. 2000. Product Chemistry of Actinovate® Iron Fulvate. Unpublished 

study prepared by Technology Sciences Group, Inc. {OPPTS 885.1100, 

885.1200, 885.1300, 885.1500, 830.1800, 830.6303, 830.6317}. 20 p. Study 

Completion Date: April 24, 2000. 

MRID 451117-01 Kuhn, J. 1999. Acute Oral Toxicity Study in Rats (Actinovate® Soluble): Final 

Report: Lab Project Number: 5199-99. Unpublished study prepared by 

Stillmeadow Labs {OPPTS 885.3050}. 10 p. Study Completion Date: October 

29, 1999. 

MRID 451117-02 Kuhn, J. 2000. Acute Pulmonary Toxicity/Pathogenicity Study in Rats with a 

Microbial Pest Control Agent (MPCA)(Soluble): Final Report: Lab Project 

Number: 5202-99. Unpublished study prepared by Stillmeadow, Inc. 41p. Study 

Completion Date: March 1, 2000. 

MRID 451117-03 Kuhn, J. 2000. Acute Injection Toxicity/Pathogenicity Study in Rats with a 

Microbial Pest Control Agent (MPCA): (Actinovate® Soluble): Final Report: Lab 

Project Number: 5203-99. Unpublished study prepared by Stillmeadow, Inc. 45 

p. Study Completion Date: March 1, 1999. 

MRID 451117-04 Kuhn, J. 1999. Primary Eye Irritation Study in Rabbits: (Actinovate® Soluble): 

Final Report: Lab Project Number: 5200-99. Unpublished study prepared by 

Stillmeadow, Inc. {OPPTS 870.2400} 16 p. Study Completion Date: September 

2, 2000. 

MRID 451117-05 Kuhn, J. 1999. Acute Dermal Irritation Study in Rabbits: (Actinovate® Soluble): 

Final Report: Lab Project Number: 5201-99. Unpublished study prepared by 

Stillmeadow, Inc. {OPPTS 870.2500}. 13 p. Study Completion Date: September 

2, 1999. 

MRID 451117-06 Sutherland, C.; Krueger, H. 2000. (Actinovate® Soluble) Streptomyces lydicus): 

A 96-Hour Static Acute Toxicity with the Rainbow Trout (Oncorhynchus mykiss): 

Final Report: Lab Project Number: 506A-101. Unpublished study prepared by 

Wildlife International, Ltd. {OPPTS 885.4200} 23 p. Study Completion Date: 

March 22, 2000. 

MRID 451413-01 Roberts, A. 2000. Product Chemistry of Actinovate® Soluble. Unpublished 

study prepared by Technology Sciences Group. {OPPTS 885.1100, 885.1200, 

885.1300, 885.1400, 885.1500, 830.6302, 830.6303, 830.6304, 830.6315, 

830.6317, 830.7000, 830.7300}. 28 p. Study Completion Date: April 24, 2000. 

MRID 460572-01 Roberts, A. 2003. Supplemental Product Chemistry for Actinovate® Soluble. 

Unpublished study prepared by Technology Sciences Group, Inc. 18 p. Study 

Completion Date: August 15, 2003. 

MRID 462980-01 Stewart, R.; Brookman, D. 2004. Product Chemistry of Actinovate® Iron Fulvate 

Supplement to MRID 45107001. Unpublished study prepared by Technology 

Sciences Group, Inc. 27 p. Study Completion Date: April 14, 2004. 
 16 

MRID 462980-02 Moore, G. 2004. Acute Oral Toxicity Up and Down Procedure in Rats: ActinoIron®. Project Number: 14129, P320/UDP. Unpublished study prepared by 

Product Safety Labs. 15 p. Study Completion Date: January 12, 2004. 

MRID 462980-03 Moore, G. 2004. Acute Dermal Toxicity Study in Rats—Limit Test: ActinoIron®. Project Number: 14130, P322. Unpublished study prepared by Product 

Safety Labs. 15 p. Study Completion Date: January 12, 2004. 

MRID 462980-04 Moore, G. 2004. Acute Inhalation Toxicity Study in Rats—Limit Test: ActinoIron®. Project Number: 14131, P330. Unpublished study prepared by Product 

Safety Labs. 23 p. Study Completion Date: January 12, 2004. 

MRID 462980-05 Moore, G. 2004. Primary Eye Irritation Study in Rabbits: Actino-Iron®. Project 

Number: 14132, P324. Unpublished study prepared by Product Safety Labs. 15 

p. Study Completion Date: January 12, 2004. 

MRID 462980-06 Moore, G. 2004. Primary Skin Irritation Study in Rabbits: Actino-Iron®. Project 

Number: 14133, P326. Unpublished study prepared by Product Safety Labs. 15 

p. Study Completion Date: January 12, 2004. 

MRID 464234-01 Brookman, D. 2004. Product Chemistry of Actinovate® Iron Fulvate 

[Addendum]. Unpublished study prepared by Technology Sciences Group, Inc. 

18 p. Study Completion Date: December 8, 2004. 

MRID 468739-01 Gallagher, S.; Beavers, J. 2005. Actinovate® Soluble: An Avian Oral 

Pathogenicity and Toxicity Study in the Northern Bobwhite: Final Report. Project 

Number: 506/101. Unpublished study prepared by Wildlife International, Ltd. 49 

p. Study Completion Date: December 15, 2005. 

MRID 468739-02 Rodrigue, N. 2006. Actinovate® Soluble: MPCA Freshwater Invertebrate 

Toxicity Test with Daphnia magna: Final Report. Project Number: 9274/05. 

Unpublished study prepared by Stillmeadow, Inc. 24 p. Study Completion 

 Date: March 16, 2006. 

MRID 468739-03 Curry, K. 2005. Response to Tier 1 Non-Target Plant Studies. Unpublished 

study prepared by Technology Sciences Group, Inc. 176 p. Study Completion 

Date: May 24, 2005. 

MRID 468739-04 Richards, K. 2006. Evaluation of the Dietary Effect(s) of Actinovate® Soluble on 

Adult Honeybees (Apis mellifera L.). Project Number: CAR/144/05, HB/1. 

Unpublished study prepared by California Agricultural Research Inc. 75 p. Study 

Completion Date: May 16, 2006. 

MRID 470434-01 Curry, K. 2006. Product Chemistry of Actinovate® STP Fungicide. Unpublished 

study prepared by Natural Industries Inc. 21 p. Study Completion Date: 

December 18, 2006. 

MRID 470434-02 Roberts, A.; Bjornson, H. 2007. Response to Tier 1 Data Requirements for 

Actinovate® STP Fungicide. Unpublished study prepared by Technology 

Sciences Group Inc. 22 p. Study Completion Date: January 29, 2007. 

 
 17 

MRID 471745-01 Roberts, A.; Bjornson, H. 2007. Supplemental Product Chemistry of 

Actinovate® Soluble: Supplemental to MRID Nos. 451413-01 and 460572-01. 

Unpublished study prepared by Natural Industries, Inc. 20 p. Study Completion 

Date: July 6, 2007. 

MRID 471876-01 Curry, K. 2007. Product Chemistry of Actinovate® STP Fungicide. Unpublished 

study prepared by Technology Sciences Group, Inc. 21 p. Study Completion 

Date: December 18, 2006. 

MRID 472205-01 Roberts, A. 2007. Supplemental Product Chemistry of Actinovate® Soluble. 

Unpublished study prepared by Natural Industries, Inc. 21 p. Study Completion 

Date: August 29, 2007. 

B. U.S. Environmental Protection Agency Risk Assessment Memoranda

U.S. EPA. 2001a. Memorandum from Robyn Rose to Alan Reynolds. Review of the submitted 

rainbow trout study and data waiver requests for Streptomyces lydicus WYEC 108. EPA Reg. No. 

073314-R; DP Barcode No. D267009; Case No. 068852; Submission No. S581481. U.S. 

Environmental Protection Agency Office of Pesticide Programs. Biopesticides and Pollution Prevention 

Division. May 25, 2001. 7 pp. 

U.S. EPA. 2001b. Memorandum from Carl Etsitty to Alan Reynolds. BPPD Review of Data Submitted 

by Natural Industries, Inc., for Registration of Actinovate Soluble and Actinovate Iron Fulvate, active 

ingredient Streptomyces lydicus WYEC 108 (Submission#: S581481, S581503; DP Barcode: 271226, 

271224; MRID Nos.: 451070-01, 451117-01, 451117-02, 451117-03, 451117-04, 451117-05, 

451413-01). U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides and 

Pollution Prevention Division. October 15, 2001. 33 pp. 

U.S. EPA. 2003a. 05-21-03. Memorandum from Joel Gagliardi to Alan Reynolds. Actinovate Iron 

Fulvate® containing Streptomyces lydicus WYEC 108. Review of February 28, 2002 responses to the 

EPA deficiency letter of November 29, 2001. U.S. Environmental Protection Agency Office of Pesticide 

Programs. Biopesticides and Pollution Prevention Division. May 21, 2003. 13 pp. 

U.S. EPA. 2003b. Memorandum from Joel Gagliardi to Alan Reynolds. Actinovate Iron Fulvate® 

containing Streptomyces lydicus WYEC 108. Review of February 28, 2003 responses to EPA inquiries 

(Alan Reynolds to Amy Roberts, request for additional information, 01/2003) pertaining to ActinovateTM 

Soluble containing Streptomyces lydicus WYEC 108, for registration of the MPCA; EPA REG. No. 

73314-R. U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides and 

Pollution Prevention Division. May 21, 2003. 6 pp. 

U.S. EPA. 2004a. 01-14-04. Memorandum from Joel Gagliardi to Alan Reynolds. Streptomyces 

lydicus WYEC 108 Food Tolerance Exemption Petition. U.S. Environmental Protection Agency Office 

of Pesticide Programs. Biopesticides and Pollution Prevention Division. January 14, 2004. 4 pp. 

U.S. EPA. 2004b. Memorandum from Joel Gagliardi to Alan Reynolds. ActinovateTM Soluble 

containing Streptomyces lydicus WYEC 108. Response to deficiencies sent to the registrant 6/4/2003 

corresponding to data originally submitted 2/28/2002 and 4/11/2003 to support registration of the MPCA 

ActinovateTM Soluble containing Streptomyces lydicus WYEC 108 . There are three responses: a 

submission 8/15/2003, a revised CSF..., and a revised label 1/8/2004, plus some interim 

correspondence. U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides 

and Pollution Prevention Division. January 14, 2004. 7 pp. 
 18 

U.S. EPA. 2004c. 02-11-04. Memorandum from Joel Gagliardi to Alan Reynolds. ActinovateTM 

Soluble containing Streptomyces lydicus WYEC 108. Review· April 24, 2000 materials from the 

submission for registration of ActinovateTM Soluble containing Streptomyces lydicus WYEC 108 for 

registration of the MPCA; EPA Reg. No. 73314- E. U.S. Environmental Protection Agency Office of 

Pesticide Programs. Biopesticides and Pollution Prevention Division. February 11, 2004. 3 pp. 

U.S. EPA. 2004d. Biopesticide Registration Action Document: Streptomyces lydicus WYEC 108 (PC 

Code 006327). U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides and 

Pollution Prevention Division. May 24, 2004. 

U.S. EPA. 2004d. Notice of Registration. Actinovate Soluble. U.S. Environmental Protection Agency 

Office of Pesticide Programs. EPA Reg. No 73314-1. May 24, 2004. 1 pp. 

U.S. EPA. 2004f. Letter from Dennis Szuhay, U.S. EPA to Robert Stewart, consultant to Natural 

Industries Inc. Actinovate® Soluble. EPA Reg. No 73314-1. Application for a label amendment dated 

June 23, 2004. Follow-up to e-mail correspondence of September 1, 2004. September 8, 2004. 

U.S. EPA. 2004g. Memorandum from Joel Gagliardi to Alan Reynolds. Actinovate Iron Fulvate® 

containing Streptomyces lydicus WYEC 108. Review submitted documents and data to support 

registration of a new end-use product Actinovate Iron Fulvate® containing Streptomyces lydicus WYEC 

108. U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides and Pollution 

Prevention Division. November 1, 2004. 20 pp. 

U.S. EPA. 2005a. Memorandum from Annabel Fellman to Alan Reynolds. Addendum to Product 

Chemistry for End-Use product Actinovate Iron Fulvate® (EPA Reg. No. 73314-E) containing 

Streptomyces lydicus WYEC 108 in response to EPA deficiency letter dated Nov. 8, 2004. U.S. 

Environmental Protection Agency Office of Pesticide Programs. Biopesticides and Pollution Prevention 

Division. January 4, 2005. 5 pp. 

U.S. EPA. 2005b. Note to File. Actinovate Iron Fulvate (EPA Reg. No. 73314-E) Response to 

Deficiencies Identified in BPPD Review (Dated 1/4/05). Alan Reynolds, Regulatory Action Leader. 

U.S. Environmental Protection Agency. January 10, 2005. 1 pp. 

U.S. EPA. 2005c. Notice of Registration. Actino-Iron Biological Fungicide. U.S. Environmental 

Protection Agency Office of Pesticide Programs. EPA Reg. No 73314-2. January 12, 2005. 

U.S. EPA. 2006a. 11-09-06. Memorandum from Tess Milofsky to Alan Reynolds. Review of avian 

oral toxicity, freshwater invertebrate toxicity, a nontarget plant waiver request, and honey bee 

information and data submitted in support of a new use site request for Actinovate Soluble, which 

contains the active ingredient Streptomyces lydicus (EPA Reg. No. 73314-4; DP No.: 330800; Decision 

No.: 368716; MRID Nos. 468739-01, 468739-02, 468739-03, and 468739-04). U.S. Environmental 

Protection Agency Office of Pesticide Programs. Biopesticides and Pollution Prevention Division. 

November 9, 2006. 17 pp. 

U.S. EPA. 2007c. Memorandum from Joel Gagliardi to Alan Reynolds. ActinovateTM Soluble and 

Actinovate® STP containing Streptomyces lydicus WYEC-108. Review updated product chemistry for 

an existing pesticide formulation and product characterization for a new end-use product formulation. 

U.S. Environmental Protection Agency Office of Pesticide Programs. Biopesticides and Pollution 

Prevention Division. October 23, 2007. 5 pp. 

U.S. EPA. 2007d. 12-7-07. Note to File: No Author. Registration Checklist. Actinovate STP. EPA 

Reg. No 73314-U. U.S. Environmental Protection Agency Office of Pesticide Programs. CSF date: 

December 7, 2007. 2 pp. 
 19 

U.S. EPA. 2007e. Notice of Registration. Actinovate STP Fungicide. U.S. Environmental Protection 

Agency Office of Pesticide Programs. EPA Reg. No 73314-4. December 19, 2007. 1 pp. 

U.S. EPA. 2014. Email from Norm Spurling to Kathleen Martin. Request For Incident Data: 

Registration Review Case 6088—Streptomyces lydicus WYEC 108. U.S. Environmental Protection 

Agency Office of Pesticide Programs. August 20, 2014 5:38 PM. 

 C. Other References

Crawford, DL et al. 1993. Isolation and Characterization of Actinomycete Antagonists of a Fungal Root 

Pathogen. Applied and Environmental Microbiology. Vol. 59, No. 11, p. 3899-3905. November 1993. 

EU. 2013. European Union. European Food Safety Authority. Conclusion on the peer review of the 

pesticide risk assessment of the active substance Streptomyces lydicus WYEC 108. EFSA Journal 

2013;11(11):3425. http://www.efsa.europa.eu/en/efsajournal/doc/3425.pdf 

EU. 2014. European Union. DG SANCO. Streptomyces lydicus WYEC 108: Status under Reg. (EC) 

No 1107/2009. Information retrieved on July 14, 2014. 

http://ec.europa.eu/sanco_pesticides/public/?event=activesubstance.detail 

NLM. 2014. Taxonomy Database. U.S. National Library Medicine. National Center for Biotechnology 

Information. Information accessed on: August 11, 2014. http://www.ncbi.nlm.nih.gov/taxonomy 

TSG. 1999. Letter from Technology Sciences Group Inc. to Alan Reynolds and Phil Hutton, U.S. EPA. 

Pre-registration meeting with Natural Industries, Inc. on the new active ingredient Streptomyces lydicus. 

April 8, 1999. 2 pp. 

TSG. 2002. Letter from Amy Plato Roberts, Technology Sciences Group Inc. to Alan Reynolds, U.S. 

EPA. ActinovateTM Soluble. File Symbol 73314-R. Response to EPA deficiency letter. February 28, 

2002. 8 pp. 

Tokala, RK et al. 2002. Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces 

lydicus WYEC 108 and the Pea Plant (Pisum sativum). Applied And Environmental Microbiology. 

Vol. 68, No. 5, p. 2161–2171. May 2002. 
 

Appendix I   

Regulatory History Pertaining to the Registration of Products

Containing S. lydicus WYEC 108 as an Active Ingredient

In April 1999, EPA met with representatives of Natural Industries Inc. met to discuss the data that would

be required to register a pesticide active ingredient that had never previously been registered (TSG 1999).  

When Natural Industries submitted their application in April 2000 the proposed use pattern was limited to

greenhouse‐ and nursery‐grown ornamentals, greenhouse‐grown crops, and turf.  The use pattern was later

expanded to include field‐grown crops and ornamentals.   

April 2000 TSG, Inc., on behalf of Natural Industries, submits an application to register two

products containing Streptomyces lydicus WYEC 108 as an active ingredient:

  ‐ Actinovate® Soluble Biological Fungicide

  ‐ Actino‐Iron® Biological Fungicide

Aug 1, 2000

65 FR 46912

Notice of Filing.  Publication of a notice announcing Natural Industries’ initial filing of a

tolerance petition (No 0F6163) proposing the establishment of a tolerance for

Streptomyces lydicus WYEC 108.

Oct 4, 2000

65 FR 59185

Notice of Receipt.  Publication of a notice announcing the receipt of Natural Industries’

application to register two pesticide products containing Streptomyces lydicus WYEC

108, an active ingredient not included in any previously registered products:

  ‐ 73314‐R:  Actinovate® Soluble Biological Fungicide; and  

  ‐ 73314‐E:  Actino‐Iron® Biological Fungicide

May 24, 2004

73314‐1

EPA registers Actinovate® Soluble Biological Fungicide (EPA Reg. No 73314‐1) for use in

greenhouses, nurseries, and turf.  The registration for this product was later amended to

include field‐grown food and ornamental crops.  U.S. EPA 2004d

June 3, 2004

69 FR 31301

Final Rule establishing an exemption from the requirement of a tolerance.  Section

180.1253 was added to 40 CFR 180 subpart D.  

Aug 4, 2004

69 FR 47144

Notice.  Publication of a notice announcing Agency approval of Natural Industries’

application to register the pesticide product Actinovate® Soluble containing an active

ingredient not included in any previously registered product.  

Jan 12, 2005

73314‐2

EPA registers Actino‐Iron® Biological Fungicide (EPA Reg. No 73314‐2) for use in

greenhouses, nurseries, and turf.  The registration for this product was later amended to

include a wider array of food and ornamentals grown in the field.  U.S. EPA 2005c

Dec 19, 2007

73314‐4

EPA registers Actinovate® STP Fungicide (EPA Reg. No 73314‐4) for use as a seed

treatment.  U.S. EPA 2007e

   
 

Appendix II 

Plant Diseases Controlled by S. lydicus WYEC 108 

Pest (genus species) Disease

Fungi

Alternaria Leaf Spot

Aphanomyces Damping Off*

Root Rot**

Armillaria Damping Off

Root Rot

Botrytis Gray Mold

Cercospora Greasy Spot

Colletotrichum Anthracnose

Diplocarpon rosae Black Spot of Rose

Erysiphe Powdery Mildew

Fusarium Brown Patch

Damping Off

Root Rot

Gaeumannomyces Take‐all Patch

Geotrichum candidum Sour Rot

Oidium Powdery Mildew

Phymatotrichum Root Rot

Puccinia Rust

Pyricularia Gray Leaf Spot

Rhizoctonia Brown Patch

Damping Off

Root Rot

Sclerotinia Damping Off

Dollar Spot

Root Rot

Sclerotium rolfsii Southern blight

Sphaerotheca Powdery Mildew

Verticillium Damping Off

Molds

Physarum cinereum Slime Mold

Peronospora Downy Mildew

Phytophthora   Damping Off

Root Rot

Pythium Damping Off

Root Rot

Bacteria

Erwinia Fire Blight

Xanthomonas Bacterial Spot

Walnut Blight

Nematodes

Belonolaimus longicaudatus Infection of crops, grasses, grains, and trees.

Tylenchulus semipenetrans Infects trees (e.g., citrus) causing general tree decline

*NOTE:  Damping off is also caused by a mold.  **NOTE:  Root Rot is also caused by a mold.

SOURCE:  The Office of Pesticide Programs OPPIN database; accessed June 23, 2014.
 

Appendix III

Use Sites for Products Containing S. lydicus WYEC 108 as an Active Ingredient

SOURCE:  The Office of Pesticide Programs OPPIN database; accessed June 23, 2014.

Crop Group Commodities

Food Uses:  commodities that are field‐, greenhouse‐, and/or residentially‐grown

Fruiting

Vegetables

and Cucurbits:

cantaloupes, cucumbers, eggplant, gourds, melons, peppers, squash, tomatillo, tomatoes  

Leafy

Vegetables

and Herbs:

basil, broccoli, Brussels sprouts, cabbage, cauliflower, celery, collards, dill, endive‐escarole,  

kale, kohlrabi, lettuce, mustard‐greens, oregano, parsley, rosemary, sage, spinach, thyme

Root and Bulb

Vegetables:

beets, carrots, garlic, ginger, ginseng, horseradish, onions, potatoes, radishes,  

sugar beets, sweet potatoes, turnips

Tree Fruits: apples, apricots, cherries, crabapples, grapefruit, lemons, limes, nectarines,

oranges, peaches, pears, plums, prunes, pummelo, quinces, tangelos, tangerines

Nuts and

Berries:

almonds, blackberries, blueberries, caneberries, currants, elderberry, filberts,  

gooseberries, huckleberries, loganberries, pecans, pistachio nuts, raspberries, walnuts

Grains and

Legumes:

barley, beans, buckwheat, corn, lentils, millet, oats, peas, rice, rye,

sorghum, soybeans, triticale, wheat

Other Food

Crops:

asparagus, avocados, bananas, canola, cotton, flax, grapes, hops, mangos, mint, mushrooms,

olives, palm, papayas, peanuts, plantain, safflower, strawberries, sunflowers, watercress  

Animal Feed,

Fodder, Hay: alfalfa, bentgrass, bermudagrass, bluegrass, clover, fescue, orchardgrass, ryegrass, trefoil, vetch

Seed

Treatment: in or on all∙raw agricultural commodities, food and fiber crops, other agricultural production crops

Nonfood:  Ornamentals that are greenhouse‐, nursery‐, outdoor‐, and/or residentially‐grown

Turf: athletic fields, cemeteries, golf courses, lawns, parks, turf grown for sod, transplant plugs

Grasses: bentgrass, bermudagrass, bluegrass, fescue, ryegrass, seashore paspalum,

St. Augustine grass, zoysiagrass

   

Other

Ornamental

Plants:

bare‐root plants, bulbs, foliage plants (cuttings and nursery) such as ferns and woody shrubs,

flowering plants (bedding and nursery) such as dichondra and roses, fruit trees, perennials

(containerized and nursery), trees