### C

- 1 Appendix C

RQ Calculations for Secondary Exposure Scenarios

### C

- 2 THEORETICAL ANALYS E S

C

- 1 . Expected Rodenticide Accumulation in Primary Consumers based on Consumption of

Bait

Rodenticide % a.i. Primary

Consumer Weight

(g) Food

intake

(g dry

wt/day) 1 Day

Accumulation

of Rodenticide

(mg a.i./kg

bwt/day) 3 Day

Accumulation

Rodenticide

(mg a.i./kg

bwt/day) 6 Day

Accumulation

Rodenticide

(mg a.i./kg

bwt/day)

Brodifacoum 0.005 House

mouse 23 3.6 7.91 23.7 47.2

Norway rat 485 20.3 2.09 6.3 12.5

Difethialone 0.0025 House

mouse 23 3.6 3.96 11.6 22.4

Norway rat 485 20.3 1.05 3.1 5.9

Warfarin 0.025 House

mouse 23 3.6 39.6 117.5 231.4

Norway rat 485 20.3 10.5 31.1 61.2

0.054 House

mouse 23 3.6 85.5 253.7 499.7

Norway rat 485 20.3 22.6 67.2 132.3

Chlorophacinone 0.005 House

mouse 23 3.6 7.91 23.3 45.2

Norway rat 485 20.3 2.09 6.2 12.0

Diphacinone 0.005 House

mouse 23 3.6 7.91 21.0 35.5

Norway rat 485 20.3 2.09 5.6 9.4

Bromethalin 0.01 House

mouse 23 3.6 15.8 42.2 71.3

Norway rat 485 20.3 4.19 9.4 18.9

### C

- 3 C

- 2. Acute Oral RQs for Secondary Birds Based on Consumption of Contaminated Carcasses

- 1 Day

Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted LD 50 (mg

a.i./kg

- bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

0.25 0.49 0.35 12.85 * 6.60 * 9.22 *

1000 58.15 181.73 1.44 0.28 0.26 5.75 * 5.15 * 5.44 *

5000 165.81 518.15 0.82 0.19 0.22 3.28 * 4.33 * 3.77 *

Norway

rat 100 12.99 40.59 0.85 0.49 0.35 3.40 * 1.75 * 2.44 *

1000 58.15 181.73 0.38 0.28 0.26 1.52 * 1.36 * 1.44 *

5000 165.81 518.15 0.22 0.19 0.22 0.87 * 1.15 * 1.00 *

Difethialone

— 0.0025%

House

mouse 100 12.99 40.59 1.61

0.30 0.28 5.35 * 5.84 *

1000 58.15 181.73 0.72 0.39 2.40 * 1.85 *

5000 165.81 518.15 0.41 0.49 1.37 * 0.83 *

Norway

rat 100 12.99 40.59 0.43 0.28 1.42 * 1.54 *

1000 58.15 181.73 0.19 0.39 0.63 * 0.49

5000 165.81 518.15 0.11 0.49 0.36 0.22

Warfarin

— 0.025% a.i.

House

mouse 100 12.99 40.59 16.06

525.00 347.02 0.03 0.05

1000 58.15 181.73 7.19 490.19 0.01 0.01

5000 165.81 518.15 4.10 624.03 0.01 0.01

Norway

rat 100 12.99 40.59 4.25 347.02 0.01 0.01

1000 58.15 181.73 1.90 490.19 0.00 0.00

5000 165.81 518.15 1.09 624.03 0.00 0.00

Warfarin

— 0.054% a.i.

House

mouse 100 12.99 40.59 34.69 525.00 347.02 0.07 0.10

1000 58.15 181.73 15.53 490.19 0.03 0.03

### C

- 4 5000 165.81 518.15 8.86 624.03 0.02 0.01

Norway

rat 100 12.99 40.59 9.18 347.02 0.02 0.03

1000 58.15 181.73 4.11 490.19 0.01 0.01

5000 165.81 518.15 2.34 624.03 0.00 0.00

Bromethalin

— 0.01%

House

mouse 100 12.99 40.59 6.42

4.60 4.22 1.40 * 1.52 *

1000 58.15 181.73 2.88 5.96 0.63 * 0.48

5000 165.81 518.15 1.64 7.59 0.36 0.22

Norway

rat 100 12.99 40.59 1.70 4.22 0.37 0.40

1000 58.15 181.73 0.76 5.96 0.17 0.13

5000 165.81 518.15 0.43 7.59 0.09 0.06

Chlorophacinone

-- 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

258.00 236.62 623.39 0.01 0.01 0.01

1000 58.15 181.73 1.44 334.24 18.40 0.01 0.00 0.07

5000 165.81 518.15 0.82 425.50 1.57 0.00 0.00 0.52*

Norway

rat 100 12.99 40.59 0.85 236.62 623.39 0.00 0.00 <0.01

1000 58.15 181.73 0.38 334.24 18.40 0.00 0.00 0.02

5000 165.81 518.15 0.22 425.50 1.57 0.00 0.00 0.14

Diphacinone

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

1630.00 1494.94 0.00 0.00

1000 58.15 181.73 1.44 2111.66 0.00 0.00

5000 165.81 518.15 0.82 2688.25 0.00 0.00

Norway

rat 100 12.99 40.59 0.85 1494.94 0.00 0.00

1000 58.15 181.73 0.38 2111.66 0.00 0.00

5000 165.81 518.15 0.22 2688.25 0.00 0.00

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the adjusted

LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For chlorophacinone, the left

and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996) and the

chlorophacinone specific scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single column gives the

adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

### C

- 5 3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exceeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.



### C

- 3 . Acute Oral RQs for Secondary Birds Based on Consumption of Contaminated Carcasses

- 3 Day

Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted LD 50 (mg a.i./kg

bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

0.25 0.49 0.35 38.46 * 19.77 * 27.6 *

1000 58.15 181.73 1.44 0.28 0.26 17.22 * 15.42 * 16.30 *

5000 165.81 518.15 0.82 0.19 0.22 9.82 * 12.96 * 11.27 *

Norway

rat 100 12.99 40.59 0.85 0.49 0.35 10.18 * 5.23 * 7.31 *

1000 58.15 181.73 0.38 0.28 0.26 4.56 * 4.08 * 4.31 *

5000 165.81 518.15 0.22 0.19 0.22 2.60 * 3.43 * 2.98 *

Difethialone

— 0.0025%

House

mouse 100 12.99 40.59 1.61

0.30 0.28 15.68 * 17.09 *

1000 58.15 181.73 0.72 0.39 7.02 * 5.42 *

5000 165.81 518.15 0.41 0.49 4.00 * 2.43 *

Norway

rat 100 12.99 40.59 0.43 0.28 4.15 * 4.52 *

1000 58.15 181.73 0.19 0.39 1.86 * 1.43 *

5000 165.81 518.15 0.11 0.49 1.06 * 0.64 *

Warfarin

— 0.025% a.i.

House

mouse 100 12.99 40.59 16.06

525.00 347.02 0.09 0.14

1000 58.15 181.73 7.19 490.19 0.04 0.04

5000 165.81 518.15 4.10 624.03 0.02 0.02

Norway

rat 100 12.99 40.59 4.25 347.02 0.02 0.04

1000 58.15 181.73 1.90 490.19 0.01 0.01

5000 165.81 518.15 1.09 624.03 0.01 0.01

### C

- 6 Warfarin

— 0.054% a.i.

House

mouse 100 12.99 40.59 34.69

525.00 347.02 0.20 0.30

1000 58.15 181.73 15.53 490.19 0.09 0.09

5000 165.81 518.15 8.86 624.03 0.05 0.04

Norway

rat 100 12.99 40.59 9.18 347.02 0.05 0.08

1000 58.15 181.73 4.11 490.19 0.02 0.02

5000 165.81 518.15 2.34 624.03 0.01 0.01

Bromethalin

— 0.01%

House

mouse 100 12.99 40.59 6.42

4.60 4.22 3.72 * 4.06 *

1000 58.15 181.73 2.88 5.96 1.67 * 1.29 *

5000 165.81 518.15 1.64 7.59 0.95 * 0.58 *

Norway

rat 100 12.99 40.59 1.70 4.22 0.98 * 1.07 *

1000 58.15 181.73 0.76 5.96 0.44 0.34

5000 165.81 518.15 0.43 7.59 0.25 0.15

Chlorophacinone

-- 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

258.00 236.62 623.39 0.04 0.04 0.01

1000 58.15 181.73 1.44 334.24 18.40 0.02 0.01 0.23

5000 165.81 518.15 0.82 425.50 1.57 0.01 0.01 1.54 *

Norway

rat 100 12.99 40.59 0.85 236.62 623.39 0.01 0.01 <0.01

1000 58.15 181.73 0.38 334.24 18.40 0.00 0.00 0.06

5000 165.81 518.15 0.22 425.50 1.57 0.00 0.00 0.41

Diphacinone

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

1630.00 1494.94 0.01 0.01

1000 58.15 181.73 1.44 2111.66 0.00 0.00

5000 165.81 518.15 0.82 2688.25 0.00 0.00

Norway

rat 100 12.99 40.59 0.85 1494.94 0.00 0.00

1000 58.15 181.73 0.38 2111.66 0.00 0.00

5000 165.81 518.15 0.22 2688.25 0.00 0.00

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the

### C

- 7 adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For

chlorophacinone, the left and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al.

(1996) and the chlorophacinone specific scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single

column gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exceeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.



### C

- 4 . Acute Oral RQs for Secondary Birds Based on Consumption of Contaminated Carcasses

- 6 Day

Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted LD 50 (mg a.i./kg

bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

0.25 0.49 0.35 76.65 * 39.40 * 55.04 *

1000 58.15 181.73 1.44 0.28 0.26 34.32 * 30.74 * 32.49 *

5000 165.81 518.15 0.82 0.19 0.22 19.57 * 25.84 * 22.47 *

Norway

rat 100 12.99 40.59 0.85 0.49 0.35 20.29 * 10.43 * 14.57 *

1000 58.15 181.73 0.38 0.28 0.26 9.08 * 8.14 * 8.60*

5000 165.81 518.15 0.22 0.19 0.22 5.18 * 6.84 * 5.95 *

Difethialone

— 0.0025%

House

mouse 100 12.99 40.59 1.61

0.30 0.28 30.25 * 32.98 *

1000 58.15 181.73 0.72 0.39 13.54 * 10.45 *

5000 165.81 518.15 0.41 0.49 7.72 * 4.68 *

Norway

rat 100 12.99 40.59 0.43 0.28 8.01 * 8.73 *

1000 58.15 181.73 0.19 0.39 3.58 * 2.77 *

5000 165.81 518.15 0.11 0.49 2.04 * 1.24 *

Warfarin

— 0.025% a.i.

House

mouse 100 12.99 40.59 16.06

525.00 347.02 0.18 0.27

1000 58.15 181.73 7.19 490.19 0.08 0.09

5000 165.81 518.15 4.10 624.03 0.05 0.04

### C

- 8 Norway

rat 100 12.99 40.59 4.25 347.02 0.05 0.07

1000 58.15 181.73 1.90 490.19 0.02 0.02

5000 165.81 518.15 1.09 624.03 0.01 0.01

Warfarin

— 0.054% a.i.

House

mouse 100 12.99 40.59 34.69

525.00 347.02 0.39 0.58 *

1000 58.15 181.73 15.53 490.19 0.17 0.19

5000 165.81 518.15 8.86 624.03 0.10 0.08

Norway

rat 100 12.99 40.59 9.18 347.02 0.10 0.15

1000 58.15 181.73 4.11 490.19 0.05 0.05

5000 165.81 518.15 2.34 624.03 0.03 0.02

Bromethalin

— 0.01%

House

mouse 100 12.99 40.59 6.42

4.60 4.22 6.29 * 6.86 *

1000 58.15 181.73 2.88 5.96 2.81 * 2.17 *

5000 165.81 518.15 1.64 7.59 1.61 * 0.97 *

Norway

rat 100 12.99 40.59 1.70 4.22 1.66 * 1.81 *

1000 58.15 181.73 0.76 5.96 0.75 * 0.58 *

5000 165.81 518.15 0.43 7.59 0.42 0.26

Chlorophacinone

-- 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

258.00 236.62 623.39 0.07 0.08 0.03

1000 58.15 181.73 1.44 334.24 18.40 0.03 0.02 0.45

5000 165.81 518.15 0.82 425.50 1.57 0.02 0.01 2.99 *

Norway

rat 100 12.99 40.59 0.85 236.62 623.39 0.02 0.02 0.01

1000 58.15 181.73 0.38 334.24 18.40 0.01 0.01 0.12

5000 165.81 518.15 0.22 425.50 1.57 0.00 0.00 0.79 *

Diphacinone

— 0.005% a.i.

House

mouse 100 12.99 40.59 3.21

1630.00 1494.94 0.01 0.01

1000 58.15 181.73 1.44 2111.66 0.00 0.00

5000 165.81 518.15 0.82 2688.25 0.00 0.00

Norway

rat 100 12.99 40.59 0.85 1494.94 0.00 0.00

1000 58.15 181.73 0.38 2111.66 0.00 0.00

### C

- 9 5000 165.81 518.15 0.22 2688.25 0.00 0.00

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the

adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For

chlorophacinone, the left and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al.

(1996) and the chlorophacinone specific scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single

column gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exceeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.



### C

- 5 . Acute Oral RQs for Secondary Mammals Based on Consumption of Contaminated

Carcasses

- 1 Day Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted

LD 50 (mg

a.i./kg

bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

0.48 0.78 6.03 * 3.71 *

1000 68.72 214.74 1.70 0.37 3.54 * 4.60 *

3000 169.54 529.80 1.40 0.28 2.91 * 4.98 *

Norway

rat 50 5.86 18.30 2.90 0.78 6.03 * 3.71 *

1000 68.72 214.74 1.70 0.37 3.54 * 4.60 *

3000 169.54 529.80 1.40 0.28 2.91 * 4.98 *

Difethialone

— 0.0025%

House

mouse 50 5.86 18.30 1.45

0.55 0.89 2.63 * 1.62 *

1000 68.72 214.74 0.85 0.42 1.54 * 2.01 *

3000 169.54 529.80 0.70 0.32 1.27 * 2.17 *

Norway

rat 50 5.86 18.30 0.38 0.89 0.70 * 0.43

1000 68.72 214.74 0.22 0.42 0.41 0.53 *

3000 169.54 529.80 0.18 0.32 0.34 0.58 *

### C

- 10 Warfarin

— 0.025% a.i.

House

mouse 50 5.86 18.30 14.48

3.00 4.88 4.83 * 2.97 *

1000 68.72 214.74 8.50 2.31 2.83 * 3.68 *

3000 169.54 529.80 6.99 1.75 2.33 * 3.99 *

Norway

rat 50 5.86 18.30 3.83 4.88 1.28 * 0.79 *

1000 68.72 214.74 2.25 2.31 0.75 * 0.97 *

3000 169.54 529.80 1.85 1.75 0.62 * 1.05 *

Warfarin

— 0.054% a.i.

House

mouse 50 5.86 18.30 31.28

3.00 4.88 10.43 * 6.41 *

1000 68.72 214.74 18.35 2.31 6.12 * 7.95 *

3000 169.54 529.80 15.09 1.75 5.03 * 8.61 *

Norway

rat 50 5.86 18.30 8.28 4.88 2.76 * 1.70 *

1000 68.72 214.74 4.86 2.31 1.62 * 2.11 *

3000 169.54 529.80 3.99 1.75 1.33 * 2.28 *

Bromethalin

— 0.01%

House

mouse 50 5.86 18.30 5.79

2.20 3.58 2.63 * 1.62 *

1000 68.72 214.74 3.40 1.69 1.54 * 2.01 *

3000 169.54 529.80 2.79 1.29 1.27 * 2.17 *

Norway

rat 50 5.86 18.30 1.53 3.58 0.70 * 0.43

1000 68.72 214.74 0.90 1.69 0.41 0.53 *

3000 169.54 529.80 0.74 1.29 0.34 0.58 *

Chlorophacinone

-- 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

6.26 10.18 0.46 0.28

1000 68.72 214.74 1.70 4.81 0.27 0.35

3000 169.54 529.80 1.40 3.66 0.22 0.38

Norway

rat 50 5.86 18.30 0.77 10.18 0.12 0.08

1000 68.72 214.74 0.45 4.81 0.07 0.09

3000 169.54 529.80 0.37 3.66 0.06 0.10

Diphacinone

— 0.005% a.i.

House 50 5.86 18.30 2.90 1.90 3.09 1.52 * 0.94 *

### C

- 11 mouse 1000 68.72 214.74 1.70 1.46 0.89 * 1.16 *

3000 169.54 529.80 1.40 1.11 0.74 * 1.26 *

Norway

rat 50 5.86 18.30 0.77 3.09 0.40 0.25

1000 68.72 214.74 0.45 1.46 0.24 0.31

3000 169.54 529.80 0.37 1.11 0.19 0.33

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the

adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For

chlorophacinone, the left and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau

et al. (1996) and the chlorophacinone specific scaling factor from Mineau et al. (2001), respectively. For other

chemicals, the single column gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exceeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.



### C

- 6 . Acute Oral RQs for Secondary Mammals Based on Consumption of Contaminated

Carcasses

- 3 Day Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted

LD 50 (mg

a.i./kg

bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

0.48 0.78 18.06 * 11.10 *

1000 68.72 214.74 1.70 0.37 10.60 * 13.78 *

3000 169.54 529.80 1.40 0.28 8.71 * 14.91 *

Norway

rat 50 5.86 18.30 2.90 0.78 4.78 * 2.94 *

1000 68.72 214.74 1.70 0.37 2.80 * 3.65 *

3000 169.54 529.80 1.40 0.28 2.31 * 3.95 *

Difethialone

— 0.0025%

House

mouse 50 5.86 18.30 1.45

0.55 0.89 7.71 * 4.74 *

1000 68.72 214.74 0.85 0.42 4.52 * 5.88 *

3000 169.54 529.80 0.70 0.32 3.72 * 6.37 *

### C

- 12 Norway

rat 50 5.86 18.30 0.38 0.89 2.04 * 1.25 *

1000 68.72 214.74 0.22 0.42 1.20 * 1.56 *

3000 169.54 529.80 0.18 0.32 0.98 * 1.68 *

Warfarin

— 0.025% a.i.

House

mouse 50 5.86 18.30 14.48

3.00 4.88 14.33 * 8.81 *

1000 68.72 214.74 8.50 2.31 8.41 * 10.93 *

3000 169.54 529.80 6.99 1.75 6.92 * 11.83 *

Norway

rat 50 5.86 18.30 3.83 4.88 3.79 * 2.33 *

1000 68.72 214.74 2.25 2.31 2.23 * 2.89 *

3000 169.54 529.80 1.85 1.75 1.83 * 3.13 *

Warfarin

— 0.054% a.i.

House

mouse 50 5.86 18.30 31.28

3.00 4.88 30.96 * 19.03 *

1000 68.72 214.74 18.35 2.31 18.16 * 23.61 *

3000 169.54 529.80 15.09 1.75 14.94 * 25.56 *

Norway

rat 50 5.86 18.30 8.28 4.88 8.19 * 5.04 *

1000 68.72 214.74 4.86 2.31 4.81 * 6.25 *

3000 169.54 529.80 3.99 1.75 3.95 * 6.76 *

Bromethalin

— 0.01%

House

mouse 50 5.86 18.30 5.79

2.20 3.58 7.02 * 4.31 *

1000 68.72 214.74 3.40 1.69 4.12 * 5.35 *

3000 169.54 529.80 2.79 1.29 3.38 * 5.79 *

Norway

rat 50 5.86 18.30 1.53 3.58 1.86 * 1.14 *

1000 68.72 214.74 0.90 1.69 1.09 * 1.42 *

3000 169.54 529.80 0.74 1.29 0.90 * 1.53 *

Chlorophacinone

-- 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

6.26 10.18 1.36 * 0.84 *

1000 68.72 214.74 1.70 4.81 0.80 * 1.04 *

3000 169.54 529.80 1.40 3.66 0.66 * 1.12 *

Norway

rat 50 5.86 18.30 0.77 10.18 0.36 0.22

1000 68.72 214.74 0.45 4.81 0.21 0.27

### C

- 13 3000 169.54 529.80 0.37 3.66 0.17 0.30

Diphacinone

— 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

1.90 3.09 4.05 * 2.49 *

1000 68.72 214.74 1.70 1.46 2.38 * 3.09 *

3000 169.54 529.80 1.40 1.11 1.96 * 3.35 *

Norway

rat 50 5.86 18.30 0.77 3.09 1.07 * 0.66 *

1000 68.72 214.74 0.45 1.46 0.63 * 0.82 *

3000 169.54 529.80 0.37 1.11 0.52 * 0.89 *

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the

adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For

chlorophacinone, the left and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau

et al. (1996) and the chlorophacinone s pecific scaling factor from Mineau et al. (2001), respectively. For other

chemicals, the single column gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exc eeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.



### C

- 7 . Acute Oral RQs for Secondary Mammals Based on Consumption of Contaminated

Carcasses

- 6 Day Accumulation in Prey

Prey

Species Predator

wt (g) Predator

FI (g dry

wt/day) Predator

FI (g wet

wt/day) Rodenticide

intake mg

a.i./kg

- bw/day Un

adjusted

LD 50 (mg a.i./

kg

- bwt) 1 Adjusted

LD 50 (mg

a.i./kg

bwt) 2 RQ

(unad

justed) 3,4 RQ

(adjusted for

weight) 4,5

Brodifacoum

— 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

0.48 0.78 36.00 * 22.13 *

1000 68.72 214.74 1.70 0.37 21.12 * 27.46 *

3000 169.54 529.80 1.40 0.28 17.37 * 29.72 *

Norway

rat 50 5.86 18.30 2.90 0.78 9.53 * 5.86 *

1000 68.72 214.74 1.70 0.37 5.59 * 7.27 *

3000 169.54 529.80 1.40 0.28 4.60 * 7.87 *

Difethialone

— 0.0025%

### C

- 14 House

mouse 50 5.86 18.30 1.45

0.55 0.89 14.88 * 9.15 *

1000 68.72 214.74 0.85 0.42 8.73 * 11.35 *

3000 169.54 529.80 0.70 0.32 7.18 * 12.28 *

Norway

rat 50 5.86 18.30 0.38 0.89 3.94 * 2.42 *

1000 68.72 214.74 0.22 0.42 2.31 * 3.00 *

3000 169.54 529.80 0.18 0.32 1.90 * 3.25 *

Warfarin

— 0.025% a.i.

House

mouse 50 5.86 18.30 14.48

3.00 4.88 28.23 * 17.35 *

1000 68.72 214.74 8.50 2.31 16.56 * 21.53 *

3000 169.54 529.80 6.99 1.75 13.62 * 23.30 *

Norway

rat 50 5.86 18.30 3.83 4.88 7.47 * 4.59 *

1000 68.72 214.74 2.25 2.31 4.38 * 5.70 *

3000 169.54 529.80 1.85 1.75 3.60 * 6.17 *

Warfarin

— 0.054% a.i.

House

mouse 50 5.86 18.30 31.28

3.00 4.88 60.97 * 37.48 *

1000 68.72 214.74 18.35 2.31 35.77 * 46.51 *

3000 169.54 529.80 15.09 1.75 29.42 * 50.33 *

Norway

rat 50 5.86 18.30 8.28 4.88 16.14 * 9.92 *

1000 68.72 214.74 4.86 2.31 9.47 * 12.31 *

3000 169.54 529.80 3.99 1.75 7.79 * 13.32 *

Chlorophacinone

-- 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

6.26 10.18 2.64 * 1.63 *

1000 68.72 214.74 1.70 4.81 1.55 * 2.02 *

3000 169.54 529.80 1.40 3.66 1.28 * 2.18 *

Norway

rat 50 5.86 18.30 0.77 10.18 0.70 * 0.43

1000 68.72 214.74 0.45 4.81 0.41 0.53 *

3000 169.54 529.80 0.37 3.66 0.34 0.58 *

Diphacinone

— 0.005% a.i.

House

mouse 50 5.86 18.30 2.90

1.90 3.09 6.83 * 4.20 *

1000 68.72 214.74 1.70 1.46 4.01 * 5.21 *

### C

- 15 3000 169.54 529.80 1.40 1.11 3.30 * 5.64 *

Norway

rat 50 5.86 18.30 0.77 3.09 1.81 * 1.11 *

1000 68.72 214.74 0.45 1.46 1.06 * 1.38 *

3000 169.54 529.80 0.37 1.11 0.87 * 1.49 *

1 LD 50 from most sensitive mallard duck or northern bobwhite quail acute oral study.

2 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the

adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For

chlorophacinone, the left and right columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau

et al. (1996) and the chlorophacinone s pecific scaling factor from Mineau et al. (2001), respectively. For other

chemicals, the single column gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996).

3 RQ calculated as daily rodenticide intake / LD 50

. 4 * RQ exceeds Acute Risk LOC.

5 Split columns show RQs calculated from the respective adjusted LD 50 , see footnote #2.

### C

- 16 C

- 8. Dietary RQs for Secondary Birds Based on Consumption of Contaminated Prey

Animals

Rodenticide % a.i. Prey Type Rodenticide concentration in

primary consumer prey

items (mg a.i./kg diet) LC 50 (mg

a.i./kg diet) RQ 2

Brodifacoum 0.005 House mouse 7.91

1.33 5.95*

Norway rat 2.09 1.57*

Difethialone 0.0025 House mouse 3.96

0.56 7.07*

Norway rat 1.05 1.87*

Warfarin 0.025 House mouse 39.6

625 0.06

Norway rat 10.5 0.02

0.054 House mouse 85.5 0.14

Norway rat 22.6 0.04

Chlorophacinone 0.005 House mouse 7.91

72 0.11

Norway rat 2.09 0.03

Diphacinone 0.005 House mouse 7.91

906 0.01

Norway rat 2.09 <0.01

Bromethalin 0.01 House mouse 15.8

210 0.08

Norway rat 4.19 0.02

Bolded (*) exceed Acute Risk LOC (0.5)

.



### C

- 9 . Dietary RQs for Secondary Mammals Based on Consumption of Contaminated

Prey Animals

Rodenticide % a.i. Prey Type Rodenticide concentration in

primary consumer prey

items (mg a.i./kg diet) LC 50 (mg

a.i./kg diet) RQ 2

Brodifacoum 0.005 House mouse 7.91

0.53 14.9*

Norway rat 2.09 3.95*

Difethialone 0.0025 House mouse 3.96

No Data --

Norway rat 1.05

--

Warfarin 0.025 House mouse 39.6

4.41 8.97*

Norway rat 10.5 2.37*

0.054 House mouse 85.5 19.4*

Norway rat 22.6 5.13*

Chlorophacinone 0.005 House mouse 7.91

1.14 6.94*

Norway rat 2.09 1.84*

Diphacinone 0.005 House mouse 7.91 2.08 3.80*

### C

- 17 Norway rat 2.09 1.00*

Bromethalin 0.01 House mouse 15.8

No data --

Norway rat 4.19

--

Bolded (*) exceed Acute Risk LOC (0.5)

.

### C

- 18 C

- 10. Secondary Birds: Number of Target Animals to Reach LD 50 

### - dose

- 1 Day Accumulation

Prey

Species Predator

wt (g) Dose in

Predator

- 1

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Dose in

Predator

- 6

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Un

adjusted

LD 50 (mg a.i./

kg

- bwt) Adjusted LD 50 (mg a.i./kg

- bwt) 1 # of Animals to

consume an un

adjusted LD 50 -

dose

- 1 Day

Accumulation # of Animals to

consume an

adjusted LD 50 - dose

1 Day

Accumulation 2

Brodifacoum

— 0.005% a.i.

House

mouse 100 1.82 10.86

0.25 0.49 0.35 0.14 0.27 0.19

1000 0.18 1.09 0.28 0.26 1.37 1.53 1.45

5000 0.04 0.22 0.19 0.22 6.87 5.20 5.98

Norway

rat 100 10.16 60.61 0.49 0.35 0.02 0.05 0.03

1000 1.02 6.06 0.28 0.26 0.25 0.27 0.26

5000 0.20 1.21 0.19 0.22 1.23 0.93 1.07

Difethialone

— 0.0025% a.i.

House

mouse 100 0.91 5.14

0.30 0.28 0.33 0.30

1000 0.09 0.51 0.39 3.30 4.27

5000 0.02 0.10 0.49 16.48 27.18

Norway

rat 100 5.08 28.70 0.28 0.06 0.05

1000 0.51 2.87 0.39 0.59 0.77

5000 0.10 0.57 0.49 2.95 4.87

Warfarin

— 0.025% a.i.

House

mouse 100 9.10 53.21

525.00 347.02 57.69 38.13

1000 0.91 5.32 490.19 576.92 538.66

5000 0.18 1.06 624.03 2884.58 3428.71

Norway

rat 100 50.79 296.99 347.02 10.34 6.83

1000 5.08 29.70 490.19 103.36 96.51

5000 1.02 5.94 624.03 516.82 614.31

Warfarin

— 0.054% a.i.

House

mouse 100 19.66 114.94 525.00 347.02 26.71 17.65

1000 1.97 11.49 490.19 267.09 249.38

### C

- 19 5000 0.39 2.30 624.03 1335.46 1587.36

Norway

rat 100 109.71 641.50 347.02 4.79 3.16

1000 10.97 64.15 490.19 47.85 44.68

5000 2.19 12.83 624.03 239.27 284.40

Bromethalin

— 0.01% a.i.

House

mouse 100 3.64 16.39

4.60 4.22 1.26 1.16

1000 0.36 1.64 5.96 12.64 16.37

5000 0.07 0.33 7.59 63.19 104.21

Norway

rat 100 20.32 91.47 4.22 0.23 0.21

1000 2.03 9.15 5.96 2.26 2.93

5000 0.41 1.83 7.59 11.32 18.67

Chlorophacinone

— 0.005% a.i.

House

mouse 100 1.82 10.40

258.00 236.62 623.39 141.76 130.01 342.52

1000 0.18 1.04 334.24 18.40 1417.57 1836.46 101.08

5000 0.04 0.21 425.50 1.57 7087.84 11689.50 43.08

Norway

rat 100 10.16 58.07 236.62 623.39 25.40 23.29 61.37

1000 1.02 5.81 334.24 18.40 253.98 329.03 18.11

5000 0.20 1.16 425.50 1.57 1269.90 2094.36 7.72

Diphacinone

— 0.005% a.i.

House

mouse 100 1.82 8.15

1630.00 1494.94 895.59 821.39

1000 0.18 0.82 2111.66 8955.95 11602.42

5000 0.04 0.16 2688.25 44779.73 73852.30

Norway

rat 100 10.16 45.51 1494.94 160.46 147.17

1000 1.02 4.55 2111.66 1604.60 2078.76

5000 0.20 0.91 2688.25 8023.02 13231.84

1 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For chlorophacinone, the left a nd right

columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996) and the chlorophacinone s pecific

scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single column gives the adjusted LD 50 using the EFED

default scaling factor from Mineau et al. (1996).

2 Split columns show number of animals necessary to reach the me dian lethal dose calculated from the respective adjusted LD 50 , see

### C

- 20 footnote #1.



### C

- 11

. Secondary Birds: Number of Target Animals to Reach LD 50 

### - dose

- 6 Day Acummulation 3

Prey

Species Predator

wt (g) Dose in

Predator

- 1

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Dose in

Predator

- 6

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Un

adjusted

LD 50 (mg a.i./

kg

- bwt) Adjusted LD 50 (mg a.i./kg

- bwt) 1 # of Animals to

consume an un

adjusted LD 50 -

dose

- 6 Day

Accumulation # of Animals to

consume an

adjusted LD 50 - dose

6 Day

Accumulation 2

Brodifacoum

— 0.005% a.i.

House

mouse 100 1.82 10.86

0.25 0.49 0.35 0.02 0.04 0.03

1000 0.18 1.09 0.28 0.26 0.23 0.26 0.24

5000 0.04 0.22 0.19 0.22 1.15 0.87 1.00

Norway

rat 100 10.16 60.61 0.49 0.35 0.00 0.01 0.01

1000 1.02 6.06 0.28 0.26 0.04 0.05 0.04

5000 0.20 1.21 0.19 0.22 0.21 0.16 0.18

Difethialone

— 0.0025% a.i.

House

mouse 100 0.91 5.14

0.30 0.28 0.06 0.05

1000 0.09 0.51 0.39 0.58 0.76

5000 0.02 0.10 0.49 2.92 4.81

Norway

rat 100 5.08 28.70 0.28 0.01 0.01

1000 0.51 2.87 0.39 0.10 0.14

5000 0.10 0.57 0.49 0.52 0.86

Warfarin

— 0.025% a.i.

House

mouse 100 9.10 53.21

525.00 347.02 9.87 6.52

1000 0.91 5.32 490.19 98.66 92.12

5000 0.18 1.06 624.03 493.32 586.38

Norway

rat 100 50.79 296.99 347.02 1.77 1.17

1000 5.08 29.70 490.19 17.68 16.51

5000 1.02 5.94 624.03 88.39 105.06

### C

- 21 Warfarin

— 0.054% a.i.

House

mouse 100 19.66 114.94

525.00 347.02 4.57 3.02

1000 1.97 11.49 490.19 45.68 42.65

5000 0.39 2.30 624.03 228.39 271.47

Norway

rat 100 109.71 641.50 347.02 0.82 0.54

1000 10.97 64.15 490.19 8.18 7.64

5000 2.19 12.83 624.03 40.92 48.64

Bromethalin

— 0.01% a.i.

House

mouse 100 3.64 9.70

4.60 4.22 0.47 0.44

1000 0.36 0.97 5.96 4.74 6.14

5000 0.07 0.19 7.59 23.72 39.11

Norway

rat 100 20.32 54.13 4.22 0.08 0.08

1000 2.03 5.41 5.96 0.85 1.10

5000 0.41 1.08 7.59 4.25 7.01

Chlorophacinone

— 0.005% a.i.

House

mouse 100 1.82 10.40

258.00 236.62 623.39 24.80 22.74 59.92

1000 0.18 1.04 334.24 18.40 247.98 321.25 17.68

5000 0.04 0.21 425.50 1.57 1239.88 2044.85 7.54

Norway

rat 100 10.16 58.07 236.62 623.39 4.44 4.07 10.74

1000 1.02 5.81 334.24 18.40 44.43 57.56 3.17

5000 0.20 1.16 425.50 1.57 222.14 366.37 1.35

Diphacinone

— 0.005% a.i.

House

mouse 100 1.82 8.15

1630.00 1494.94 199.89 183.32

1000 0.18 0.82 2111.66 1998.86 2589.52

5000 0.04 0.16 2688.25 9994.28 16482.92

Norway

rat 100 10.16 45.51 1494.94 35.81 32.85

1000 1.02 4.55 2111.66 358.13 463.95

5000 0.20 0.91 2688.25 1790.64 2953.18

1 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. F or chlorophacinone, the left and right

### C

- 22 columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996) and the chlorophacinone specific

scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single column gives the adjusted LD 50 using the EFED

default scaling factor from Mineau et al. (1996).

2 Split columns show number of animals necessary to reach the median lethal dose calculated from t he respective adjusted LD 50 , see

footnote #1.

3 Due to bromethalin’s rapid mode of action, numbers or individuals necessary to reach the median lethal dose are based on thre e day

accumulation in prey.



### C

- 12

. Secondary Mammals: Number of Target Animals to Reach LD 50 

### - dose

- 1 Day Accumulation

Prey

Species Predator

wt (g) Dose in

Predator

- 1

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Dose in

Predator

- 6

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Un

adjusted

LD 50 (mg a.i./

kg

- bwt) Adjusted LD 50 (mg a.i./kg

- bwt) 1 # of Animals to

consume an un

adjusted LD 50 -

dose

- 1 Day

Accumulation # of Animals to

consume an

adjusted LD 50 - dose

1 Day

Accumulation 2

Brodifacoum

— 0.005% a.i.

House

mouse 100 3.64 21.72

0.48 0.78 0.13 0.21

1000 0.18 1.09 0.37 2.64 2.03

5000 0.06 0.36 0.28 7.91 4.62

Norway

rat 100 20.32 121.21 0.78 0.02 0.04

1000 1.02 6.06 0.37 0.47 0.36

5000 0.34 2.02 0.28 1.42 0.83

Difethialone

— 0.0025% a.i.

House

mouse 100 1.82 10.28

0.55 0.89 0.30 0.49

1000 0.09 0.51 0.42 6.04 4.65

5000 0.03 0.17 0.32 18.13 10.60

Norway

rat 100 10.16 57.40 0.89 0.05 0.09

1000 0.51 2.87 0.42 1.08 0.83

5000 0.17 0.96 0.32 3.25 1.90

Warfarin

— 0.025% a.i.

House

mouse 100 18.20 106.42 3.0 4.88 0.16 0.27

1000 0.91 5.32 2.31 3.30 2.54

### C

- 23 5000 0.30 1.77 1.75 9.89 5.78

Norway

rat 100 101.58 593.98 4.88 0.03 0.05

1000 5.08 29.70 2.31 0.59 0.45

5000 1.69 9.90 1.75 1.77 1.04

Warfarin

— 0.054% a.i.

House

mouse 100 39.31 229.87

3.0 4.88 0.08 0.12

1000 1.97 11.49 2.31 1.53 1.17

5000 0.66 3.83 1.75 4.58 2.68

Norway

rat 100 219.42 1283.00 4.88 0.01 0.02

1000 10.97 64.15 2.31 0.27 0.21

5000 3.66 21.38 1.75 0.82 0.48

Bromethalin

— 0.01% a.i.

House

mouse 100 7.28 32.78

2.2 3.58 0.30 0.49

1000 0.36 1.64 1.69 6.04 4.65

5000 0.12 0.55 1.29 18.13 10.60

Norway

rat 100 40.63 182.94 3.58 0.05 0.09

1000 2.03 9.15 1.69 1.08 0.83

5000 0.68 3.05 1.29 3.25 1.90

Chlorophacinone

— 0.005% a.i.

House

mouse 100 3.64 20.81

6.26 10.18 1.72 2.80

1000 0.18 1.04 4.81 34.40 26.46

5000 0.06 0.35 3.66 103.19 60.31

Norway

rat 100 20.32 116.14 10.18 0.31 0.50

1000 1.02 5.81 4.81 6.16 4.74

5000 0.34 1.94 3.66 18.49 10.80

Diphacinone

— 0.005% a.i.

House

mouse 100 3.64 16.31

1.90 3.09 0.52 0.85

1000 0.18 0.82 1.46 10.44 8.03

5000 0.06 0.27 1.11 31.32 18.30

Norway 100 20.32 91.03 3.09 0.09 0.15

### C

- 24 rat 1000 1.02 4.55 1.46 1.87 1.44

5000 0.34 1.52 1.11 5.61 3.28

1 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For chlorophacinone, the left a nd right

columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996) and the chlorophacinone specific

scaling factor from Mineau et al. (2001), respectively. For other chemicals, the single column gives the adjusted LD 50 using the EFED

default scaling factor from Mineau et al. (1996).

2 Split columns show num ber of animals necessary to reach the median lethal dose calculated from the respective adjusted LD 50 , see

footnote #1.



### C

- 13

. Secondary Mammals: Number of Target Animals to Reach LD 50 

### - dose

- 6 Day Accumulation 3

Prey

Species Predator

wt (g) Dose in

Predator

- 1

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Dose in

Predator

- 6

Day

Accumulation

in Prey (mg

a.i./kg

- bwt) Un

adjusted

LD 50 (mg a.i./

kg

- bwt) Adjusted LD 50 (mg a.i./kg

- bwt) 1 # of Animals to

consume an un

adjusted LD 50 -

dose

- 6 Day

Accumulation # of Animals to

consume an

adjusted LD 50 - dose

6 Day

Accumulation 2

Brodifacoum

— 0.005% a.i.

House

mouse 100 3.64 21.72

0.48 0.78 0.02 0.04

1000 0.18 1.09 0.37 0.44 0.34

5000 0.06 0.36 0.28 1.33 0.78

Norway

rat 100 20.32 121.21 0.78 0.00 0.01

1000 1.02 6.06 0.37 0.08 0.06

5000 0.34 2.02 0.28 0.24 0.14

Difethialone

— 0.0025% a.i.

House

mouse 100 1.82 10.28

0.55 0.89 0.05 0.09

1000 0.09 0.51 0.42 1.07 0.82

5000 0.03 0.17 0.32 3.21 1.88

Norway

rat 100 10.16 57.40 0.89 0.01 0.02

1000 0.51 2.87 0.42 0.19 0.15

5000 0.17 0.96 0.32 0.57 0.34

Warfarin

— 0.025% a.i.

### C

- 25 House

mouse 100 18.20 106.42

3.0 4.88 0.03 0.05

1000 0.91 5.32 2.31 0.56 0.43

5000 0.30 1.77 1.75 1.69 0.99

Norway

rat 100 101.58 593.98 4.88 0.01 0.01

1000 5.08 29.70 2.31 0.10 0.08

5000 1.69 9.90 1.75 0.30 0.18

Warfarin

— 0.054% a.i.

House

mouse 100 39.31 229.87

3.0 4.88 0.01 0.02

1000 1.97 11.49 2.31 0.26 0.20

5000 0.66 3.83 1.75 0.78 0.46

Norway

rat 100 219.42 1283.00 4.88 0.00 0.00

1000 10.97 64.15 2.31 0.05 0.04

5000 3.66 21.38 1.75 0.14 0.08

Bromethalin

— 0.01% a.i.

House

mouse 100 7.28 19.40

2.2 3.58 0.11 0.18

1000 0.36 0.97 1.69 2.27 1.74

5000 0.12 0.32 1.29 6.81 3.98

Norway

rat 100 40.63 108.26 3.58 0.02 0.03

1000 2.03 5.41 1.69 0.41 0.31

5000 0.68 1.80 1.29 1.22 0.71

Chlorophacinone

— 0.005% a.i.

House

mouse 100 3.64 20.81

6.26 10.18 0.30 0.49

1000 0.18 1.04 4.81 6.02 4.63

5000 0.06 0.35 3.66 18.05 10.55

Norway

rat 100 20.32 116.14 10.18 0.05 0.09

1000 1.02 5.81 4.81 1.08 0.83

5000 0.34 1.94 3.66 3.23 1.89

Diphacinone

— 0.005% a.i.

House

mouse 100 3.64 16.31 1.90 3.09 0.12 0.19

1000 0.18 0.82 1.46 2.33 1.79

### C

- 26 5000 0.06 0.27 1.11 6.99 4.09

Norway

rat 100 20.32 91.03 3.09 0.02 0.03

1000 1.02 4.55 1.46 0.42 0.32

5000 0.34 1.52 1.11 1.25 0.73

1 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right columns give the adjusted LD 50 using brodifacoum specific scaling factor from Mineau et al. (1996) and (2001), respectively. For chlorophacinone, the left and right

columns gives the adjusted LD 50 using the EFED default scaling factor from Mineau et al. (1996) and the chlorophacinone specific

scaling factor from Mineau et al. (2001), resp ectively. For other chemicals, the single column gives the adjusted LD 50 using the EFED

default scaling factor from Mineau et al. (1996).

2 Split columns show number of animals necessary to reach the median lethal dose calculated from the respective adjust ed LD 50 , see

footnote #1.

3 Due to bromethalin’s rapid mode of action, numbers or individuals necessary to reach the median lethal dose are based on thre e day

accumulation in prey.

### C

- 27 EMPERICAL ANALYSE S

C

- 14 . Expected Rodenticide Intakes for Carnivorous Birds based on Consumption of

Contaminated Carcasses

Rodenticide Weight (g) Food intake

(g dry

- wt/day) Food intake

(g wet

- wt/day) Rodenticide intake

(mg a.i./kg

- bwt/day) 1

Brodifacoum 100 12.99 40.59 2.83

1000 58.15 181.73 1.27

5000 165.81 518.15 0.72

Difethialone 100 12.99 40.59 1.26

1000 58.15 181.73 0.56

5000 165.81 518.15 0.32

Warfarin 100 12.99 40.59 1.20

1000 58.15 181.73 0.54

5000 165.81 518.15 0.31

Chlorophacinone 100 12.99 40.59 0.64

1000 58.15 181.73 0.29

5000 165.81 518.15 0.16

Diphacinone 100 12.99 40.59 0.57

1000 58.15 181.73 0.25

5000 165.81 518.15 0.15

1 See Table 3.8 for derivation.



### C

- 1 5 . Expected Rodenticide Intakes for Carnivorous Mammals based on Consumption of

Contaminated Carcasses

Rodenticide Weight (g) Food intake

(g dry

- wt/day) Food intake

(g wet

- wt/day) Rodenticide intake

(mg a.i./kg

- bwt/day) 1

Brodifacoum 50 5.9 18.3 2.55

1000 68.7 214.7 1.50

3000 169.5 529.8 1.23

Difethialone 50 5.9 18.3 1.13

1000 68.7 214.7 0.67

3000 169.5 529.8 0.55

Warfarin 50 5.9 18.3 1.10

### C

- 28 1000 68.7 214.7 0.64

3000 169.5 529.8 0.53

Chlorophacinone 50 5.86 18.30 0.58

1000 68.72 214.74 0.34

3000 169.54 529.80 0.28

Diphacinone 50 5.86 18.30 0.51

1000 68.72 214.74 0.30

3000 169.54 529.80 0.25

1 See Table 3.8 for derivation.



### C

- 1 6 . Carnivorous/Scavenger Bird Acute RQs based on a Single

- dose of

Rodenticide by Gavage, consumption of contaminated carcasses

Rodenticide Weight (g) Rodenticide

intake (mg

a.i./kg

- bwt/day) 1 LD 50 (mg

a.i./kg

- bwt) Adjusted LD 50

(mg a.i./kg

bwt) 2,3 RQ 4,5

Brodifacoum 100 2.83 0.25 0.44 0.33 6.42* 8.54*

1000 1.27 0.25 0.25 0.25 5.01* 5.04*

5000 0.72 0.25 0.17 0.21 4.21* 3.49*

Difethialone 100 1.26 0.30 0.27 4.64*

1000 0.56 0.30 0.38 1.47*

5000 0.32 0.30 0.49 0.66*

Warfarin 100 1.20 525 347.02 < 0.01

1000 0.54 525 490.19 < 0.01

5000 0.31 525 624.03 < 0.01

Chlorophacinone 100 0.64 258 232.00 726.23 < 0.01 < 0.01

1000 0.29 258 327.71 22.50 < 0.01 0.01

5000 0.16 258 417.20 1.92 < 0.01 0.09

Diphacinone 100 0.57 1630 1480.38 < 0.01

1000 0.25 1630 2091.10 < 0.01

5000 0.15 1630 2662.07 < 0.01

1 See Tables 3.8 and 3.9 for derivation.

2 See Table 5.2 for derivation with assessed body weights used in this table.

3 Split columns show LD 50 s adjusted using different scaling factors. For brodifacoum, the left and right

columns give the adjusted LD 50 using chemical specific scaling factor from Mineau et al. (1996) and

(2001), respectively.

### C

- 29 4 Split columns show RQs calculated from the respective adjusted LD 50

.

Bolded (*) exceed Acute Risk LOC (0.5)

.



### C

- 1 7 . Carnivorous/Scavenger Mammal Acute RQs based on a Single

- dose of

Rodenticide by Gavage, consumption of contaminated carcasses

Rodenticide Weight (g) Rodenticide

intake (mg

a.i./kg

- bwt/day) 1 LD 50 (mg

a.i./kg

- bwt) Adjusted LD 50

(mg a.i./kg

bwt) 2 RQ 3

(adjusted for

weight)

Brodifacoum 50 2.55 0.48 0.71 3.59*

1000 1.50 0.48 0.34 4.46*

3000 1.23 0.48 0.26 4.83*

Difethialone 50 1.13 0.55 0.81 1.41*

1000 0.67 0.55 0.38 1.75*

3000 0.55 0.55 0.29 1.89*

Warfarin 50 1.08 3.0 4.22 0.26

1000 0.63 3.0 2.00 0.32

3000 0.52 3.0 1.52 0.34

Chlorophacinone 50 0.58 6.26 8.56 0.07

1000 0.34 6.26 4.05 0.08

3000 0.28 6.26 3.08 0.08

Diphacinone 50 0.51 2.30 3.35 0.15

1000 0.30 2.30 1.58 0.19

3000 0.25 2.30 1.20 0.21

1 See Tables 3.8 and 3.9 for derivation.

2 See Table 5.2 for derivation with assessed body weights used in this table.

Bolded (*) exceed Acute Risk LOC (0.5)

.



### C

- 1 8 . Bird Acute RQs based on a 5

- day Exposure to Rodenticide in the Diet, consumption

of contaminated carcasses

Rodenticide Warfarin

concentration in

carcass (mg a.i./kg

carcass) LC 50

(mg a.i./kg

- diet) RQ 1

Brodifacoum 6.98 1.33 5.25*

Difethialone 3.10 0.56 5.54*

Warfarin 2.95 625 < 0.01

### C

- 30 Chlorophacinone 1.58 72 0.02

Diphacinone 1.40 906 < 0.01

Bolded (*) exceed Acute Risk LOC (0.5)

.



### C

- 1 9 . Mammal Acute RQs based on a 5

- day Exposure to Rodenticide in the Diet,

consumption of contaminated carcasses

Rodenticide Warfarin

concentration in

carcass (mg a.i./kg

carcass) LC 50

(mg a.i./kg

- diet) RQ 1

Brodifacoum 6.98 0.53 13.17*

Warfarin 2.95 4.41 0.67*

Chlorophacinone 1.58 1.14 1.39*

Diphacinone 1.40 2.31 0.61*

Bolded (*) exceed Acute Risk LOC (0.5)

.