MDL: SM 4500-F D MDL Lab 1 MDL Lab 2 MDL Lab 3

LIS 0.078425202 0.023736169 0.111753419 HIS 0.056917334 0.05566625 0.053516157 MDL2 Lab 1 Lab 2 Lab 3 LIS 0.006150512 0.000563406 0.012489 HIS 0.003239583 0.003098731 0.002864 MDL pooled (mg/L) SM 4500-F D SPADNS II LIS 0.065 0.041 HIS 0.045 0.034 ML pooled (mg/L) SM 4500-F D SPADNS II LIS 0.207 0.129 HIS 0.143 0.107 Average % Recovery: SM 4500-F D SPADNS II LIS 100.0% 100.0% HIS 100.6% 102.9% Between laboratory Std Dev (sb) SM 4500-F D SPADNS II LIS 0.043 0.011 HIS 0.049 0.027 Lab 1 Avg Lab 2 Avg Lab 3 Avg SM 4500-F D LIS 1.03 1.02 0.95 HIS 1.00 1.06 0.96 SPADNS II LIS 1.01 1.00 0.99 HIS 1.06 1.02 1.00 Pooled within-laboratory Std Dev (sw) SM 4500-F D SPADNS II LIS 0.021 0.022 HIS 0.020 0.018 Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev SM 4500-F D LIS 0.03 0.01 0.02 HIS 0.03 0.01 0.02 SPADNS II LIS 0.03 0.01 0.02 HIS 0.02 0.01 0.02 Precision Acceptance Criteria F = √3.16 1.777638883RSD SM 4500-F D SPADNS II LIS 0.021 0.022 HIS 0.020 0.017 IPR QC @ 95%Conf SM 4500-F D SPADNS II LIS 0.037 0.038 HIS 0.035 0.031 Recovery Acceptance Criteria (sc) SM 4500-F D SPADNS II LIS 0.050 0.015 HIS 0.057 0.031 (1+1/m) 1.333333333 (1/4-1/n) 0.107142857 QC Acceptance criteria (IPR) SM 4500-F D SPADNS II Lower Limit (LIS) 84% 95% Lower Limit (HIS) 82% 93% Upper Limit (LIS) 116% 105% Upper Limit (HIS) 119% 113% Matrix SM 4500-F D Lab 1 Lab 2 Lab 3 Average Conc (mg/L) 0.93 0.96 1.22 Std Dev (7 reps) 0.022 0.007 0.027 % RSD (7reps) 2.4% 0.7% 2.2% SPADNS II Lab 1 Lab 2 Lab 3 Average Conc (mg/L) 0.90 0.94 1.23 Std Dev (7 reps) 0.024 0.005 0.017 % RSD (7reps) 2.7% 0.6% 1.4%

## Roseville

SM4500-F D MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.09 0.025 0.078 0.249 HIS 0.12 0.018 0.057 0.181 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 108% 102% 101% 101% HIS 104% 96% 100% 99% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 0.95 0.91 0.95 0.92 SPADNS 2 MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.06 0.011 0.035 0.111 HIS 0.11 0.015 0.048 0.153 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 99% 99% 104% 106% HIS 107% 104% 104% 105% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 0.94 0.87 0.90 0.89Rounded ML 0.30 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

106% 103% 100% 103% 0.029 101% 103% 99% 100% 0.027 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.94 0.89 0.93 0.93 0.022 2.4%

Rounded ML

0.10 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

102% 100% 99% 101% 0.028 106% 109% 105% 106% 0.018 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.91 0.93 0.89 0.90 0.024 2.7%

## Loveland

SM4500-F D MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.09 0.008 0.024 0.075 HIS 0.14 0.018 0.056 0.177 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 102% 102% 102% 100% HIS 107% 107% 106% 105% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 0.95 0.96 0.96 0.96 SPADNS 2 MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.06 0.007 0.022 0.069 HIS 0.13 0.006 0.018 0.058 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 101% 100% 99% 98% HIS 104% 102% 103% 101% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 0.94 0.94 0.94 0.95Rounded ML 0.10 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

102% 102% 103% 102% 0.009 104% 105% 106% 106% 0.011 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.97 0.96 0.97 0.96 0.007 0.7%

Rounded ML

0.10 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

99% 100% 100% 100% 0.010 102% 103% 102% 102% 0.010 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.94 0.95 0.95 0.94 0.005 0.6%

## Storm Lake

SM4500-F D MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.18 0.036 0.112 0.355 HIS 0.09 0.017 0.054 0.170 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 95% 97% 95% 98% HIS 98% 97% 93% 96% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 1.21 1.22 1.25 1.22 SPADNS 2 MDL Study: 0.1 mg/L Spike Average (mg/L) Std. Dev. MDL ML LIS 0.08 0.024 0.076 0.243 HIS 0.11 0.016 0.050 0.160 IPR Study: 1.0 mg/L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 103% 98% 96% 101% HIS 104% 99% 101% 97% Matrix Conc. (mg/L) Rep 1 Rep 2 Rep 3 Rep 4 1.25 1.22 1.22 1.22Rounded ML 0.40 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

93% 93% 94% 95% 0.019 94% 96% 97% 96% 0.018 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

1.25 1.17 1.22 1.22 0.027 2.2%

Rounded ML

0.20 0.20 % Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

99% 99% 98% 99% 0.023 99% 102% 101% 100% 0.023 Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

1.24 1.24 1.2 1.23 0.017 1.4%

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Stephen Peterson Facility Name: City

of Roseville Water Treatment Plant City, State: Roseville, Ca Calibration Check 0.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 1.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 

### 1. Standard Preparation - Chlorine Concentrations 1.1.5 Actual Chlorine Concentration: 4.72 (mg/L Cl2) 1.2.5 Actual Chlorine Concentration: 5.02 (mg/L Cl2) 1.3.5 Actual Chlorine Concentration: 4.9 (mg/L Cl2) 1.4.5 Actual Chlorine Concentration: 4.84 (mg/L Cl2) 1.53 2% 1.47 2% 0.57 14% 0.44 12%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

Data: 0.13 0.09 0.06 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.07 0.08 0.11 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength Data: 0.06 0.05 0.07 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.07 0.05 0.08 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 0.14 0.1 0.14 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.13 0.12 0.1 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

Data: 0.1 0.1 0.14 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.1 0.11 0.1 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength Data: 1.08 1.02 1.01 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.06 1.03 1 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength Data: 0.99 0.99 1.04Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F)

1.02 1 0.99 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 1.04 0.96 1 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.01 1.03 0.99 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength Data: 1.07 1.04 1.04 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.06 1.09 1.05 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

### 4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent

Data: 0.95 0.91 0.95 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.94 0.89 0.93 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent Data: 0.94 0.87 0.9 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.91 0.93 0.89 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference 1.55 3% 1.49 1% 0.55 10% 0.46 8%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

0.07 Sample 4 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength 0.06 Sample 4 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 0.14 Sample 4 (mg/L F)

### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

0.12 Sample 4 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength 1.01 Sample 4 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength 1.06Sample 4 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 0.99 Sample 4 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength 1.05 Sample 4 (mg/L F)0.92

Sample 4 (mg/L F)

0.89 Sample 4 (mg/L F)

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Pat Wiese Facility Name: Hach Company City, State: Loveland, CO Calibration Check 0.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 1.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 

### 1. Standard Preparation - Chlorine Concentrations 1.1.5 Actual Chlorine Concentration: 4.5 (mg/L Cl2) 1.2.5 Actual Chlorine Concentration: 4.8 (mg/L Cl2) 1.3.5 Actual Chlorine Concentration: 4.4 (mg/L Cl2) 1.4.5 Actual Chlorine Concentration: 4.5 (mg/L Cl2) 1.51 0.7% 1.50 0% 0.56 12% 0.55 10%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

Data: 0.10 0.10 0.09 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.09 0.08 0.10 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength Data: 0.06 0.07 0.06 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.05 0.07 0.06 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 0.11 0.16 0.15 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.13 0.16 0.14Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength Data: 0.14 0.13 0.13 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.13 0.13 0.12 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength Data: 1.02 1.02 1.02 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.02 1.02 1.03 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength Data:1.01 1.00 0.99

Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.99 1.00 1.00 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 1.07 1.07 1.06 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.04 1.05 1.06 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength Data: 1.04 1.02 1.03 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.02 1.03 1.02 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

### 4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent

Data: 0.95 0.96 0.96 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.97 0.96 0.97 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent Data: 0.94 0.94 0.94 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.94 0.95 0.95 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference 1.51 0.7% 1.49 0.7% 0.55 10% 0.55 10%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

0.09 Sample 4 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength 0.06 Sample 4 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 0.14 Sample 4 (mg/L F)

### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

0.13 Sample 4 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength 1.00 Sample 4 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength0.98

Sample 4 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 1.05 Sample 4 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength 1.01 Sample 4 (mg/L F)0.96

Sample 4 (mg/L F)

0.95 Sample 4 (mg/L F)

### Drinking Water Sample Matrix Validation with Distillation - Loveland, CO

SPADNS Data: Replicate 1 Replicate 2 Replicate 3 Replicate 4 SPADNS 2 Data: Replicate 1 Replicate 2 Replicate 3 Replicate 4 0.90 0.98 1.09 1.01 0.92 0.99 1.10 1.02Hach 10225 Standard Standard Average Result Deviation Average Result Deviation Distillation Results (mg/L F) 1.01 0.075 1.00 0.079 SM 4500-F D

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Cory Sutton Facility Name: Storm Lake Water Treatment Plant City, State: Storm Lake, Iowa Calibration Check 0.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 1.5 mg/L F- standard solution:

SPADNS: Replicate 1 (mg/L F) % Difference SPADNS 2: Replicate 1 (mg/L F) % Difference 

### 1. Standard Preparation - Chlorine Concentrations 1.1.5 Actual Chlorine Concentration: 3.4 (mg/L Cl2) 1.2.5 Actual Chlorine Concentration: 2.8 (mg/L Cl2) 1.3.5 Actual Chlorine Concentration: 2.6 (mg/L Cl2) 1.4.5 Actual Chlorine Concentration: 2.1 (mg/L Cl2) 1.53 2% 1.60 7% 0.59 18% 0.58 16%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

Data: 0.15 0.14 0.15 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.19 0.22 0.23 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength Data: 0.11 0.10 0.04 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.07 0.07 0.09 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 0.11 0.07 0.10 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.09 0.11 0.07Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength Data: 0.10 0.12 0.10 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.08 0.11 0.13 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength Data: 0.95 0.97 0.95 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.93 0.93 0.94 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength Data:1.03 0.98 0.96

Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.99 0.99 0.98 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength Data: 0.98 0.97 0.93 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.94 0.96 0.97 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength Data: 1.04 0.99 1.01 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 0.99 1.02 1.01 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

### 4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent

Data: 1.21 1.22 1.25 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.25 1.17 1.22 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)



### 4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent Data: 1.25 1.22 1.22 Sample 1 (mg/L F) Sam ple 2 (mg/L F) Sam ple 3 (mg/L F) 1.24 1.24 1.20 Sample 5 (mg/L F) Sam ple 6 (mg/L F) Sam ple 7 (mg/L F)

## Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference Replicate 2 (mg/L F) % Difference 1.53 2% 1.58 5% 0.60 20% 0.57 14%

### 2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Strength

0.18 Sample 4 (mg/L F)



### 2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Strength 0.10 Sample 4 (mg/L F)



### 2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 0.10 Sample 4 (mg/L F)

### 2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

0.11 Sample 4 (mg/L F)



### 3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strength 0.98 Sample 4 (mg/L F)



### 3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strength1.01

Sample 4 (mg/L F)



### 3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strength 0.96 Sample 4 (mg/L F)



### 3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength 0.97 Sample 4 (mg/L F)1.22

Sample 4 (mg/L F)

1.22 Sample 4 (mg/L F)