MDL: SM 4500-F D

MDL Lab 1 MDL Lab 2 MDL Lab 3

LIS 0.078425202 0.023736169 0.111753419

HIS 0.056917334 0.05566625 0.053516157

MDL2

Lab 1 Lab 2 Lab 3

LIS 0.006150512 0.000563406 0.012489

HIS 0.003239583 0.003098731 0.002864

MDL pooled (mg/L)

SM 4500-F D SPADNS II

LIS 0.065 0.041

HIS 0.045 0.034

ML pooled (mg/L)

SM 4500-F D SPADNS II

LIS 0.207 0.129

HIS 0.143 0.107

Average % Recovery:

SM 4500-F D SPADNS II

LIS 100.0% 100.0%

HIS 100.6% 102.9%

Between laboratory Std Dev (sb)

SM 4500-F D SPADNS II

LIS 0.043 0.011

HIS 0.049 0.027

Lab 1 Avg Lab 2 Avg Lab 3 Avg

SM 4500-F D

LIS 1.03 1.02 0.95

HIS 1.00 1.06 0.96

SPADNS II

LIS 1.01 1.00 0.99

HIS 1.06 1.02 1.00

Pooled within-laboratory Std Dev (sw)

SM 4500-F D SPADNS II

LIS 0.021 0.022

HIS 0.020 0.018

Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev

SM 4500-F D

LIS 0.03 0.01 0.02

HIS 0.03 0.01 0.02

SPADNS II

LIS 0.03 0.01 0.02

HIS 0.02 0.01 0.02

Precision Acceptance Criteria

F = √3.16 1.777638883
RSD SM 4500-F D SPADNS II

LIS 0.021 0.022

HIS 0.020 0.017

IPR QC @ 95% Conf

SM 4500-F D SPADNS II

LIS 0.037 0.038

HIS 0.035 0.031

Recovery Acceptance Criteria (sc)

SM 4500-F D SPADNS II

LIS 0.050 0.015

HIS 0.057 0.031

(1+1/m) 1.333333333

(1/4-1/n) 0.107142857

QC Acceptance criteria (IPR)

SM 4500-F D SPADNS II

Lower Limit (LIS) 84% 95%

Lower Limit (HIS) 82% 93%

Upper Limit (LIS) 116% 105%

Upper Limit (HIS) 119% 113%

Matrix

SM 4500-F D

Lab 1 Lab 2 Lab 3

Average Conc (mg/L) 0.93 0.96 1.22

Std Dev (7 reps) 0.022 0.007 0.027

% RSD (7reps) 2.4% 0.7% 2.2%

SPADNS II

Lab 1 Lab 2 Lab 3

Average Conc (mg/L) 0.90 0.94 1.23

Std Dev (7 reps) 0.024 0.005 0.017

% RSD (7reps) 2.7% 0.6% 1.4%
Roseville

SM4500-F D

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.09 0.025 0.078 0.249

HIS 0.12 0.018 0.057 0.181

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 108% 102% 101% 101%

HIS 104% 96% 100% 99%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

0.95 0.91 0.95 0.92

SPADNS 2

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.06 0.011 0.035 0.111

HIS 0.11 0.015 0.048 0.153

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 99% 99% 104% 106%

HIS 107% 104% 104% 105%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

0.94 0.87 0.90 0.89
Rounded ML

0.30

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

106% 103% 100% 103% 0.029

101% 103% 99% 100% 0.027

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.94 0.89 0.93 0.93 0.022 2.4%

Rounded ML

0.10

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

102% 100% 99% 101% 0.028

106% 109% 105% 106% 0.018

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.91 0.93 0.89 0.90 0.024 2.7%
Loveland

SM4500-F D

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.09 0.008 0.024 0.075

HIS 0.14 0.018 0.056 0.177

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 102% 102% 102% 100%

HIS 107% 107% 106% 105%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

0.95 0.96 0.96 0.96

SPADNS 2

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.06 0.007 0.022 0.069

HIS 0.13 0.006 0.018 0.058

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 101% 100% 99% 98%

HIS 104% 102% 103% 101%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

0.94 0.94 0.94 0.95
Rounded ML

0.10

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

102% 102% 103% 102% 0.009

104% 105% 106% 106% 0.011

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.97 0.96 0.97 0.96 0.007 0.7%

Rounded ML

0.10

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

99% 100% 100% 100% 0.010

102% 103% 102% 102% 0.010

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

0.94 0.95 0.95 0.94 0.005 0.6%
Storm Lake

SM4500-F D

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.18 0.036 0.112 0.355

HIS 0.09 0.017 0.054 0.170

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 95% 97% 95% 98%

HIS 98% 97% 93% 96%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

1.21 1.22 1.25 1.22

SPADNS 2

MDL Study: 0.1 mg/L Spike

Average (mg/L) Std. Dev. MDL ML

LIS 0.08 0.024 0.076 0.243

HIS 0.11 0.016 0.050 0.160

IPR Study: 1.0 mg/L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4

LIS 103% 98% 96% 101%

HIS 104% 99% 101% 97%

Matrix Conc. (mg/L)

Rep 1 Rep 2 Rep 3 Rep 4

1.25 1.22 1.22 1.22
Rounded ML

0.40

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

93% 93% 94% 95% 0.019

94% 96% 97% 96% 0.018

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

1.25 1.17 1.22 1.22 0.027 2.2%

Rounded ML

0.20

0.20

% Recovery #5 % Recovery #6 % Recovery #7 Avg % Recovery Std. Dev.

99% 99% 98% 99% 0.023

99% 102% 101% 100% 0.023

Rep 5 Rep 6 Rep 7 Avg. Conc. Std. Dev. % RSD

1.24 1.24 1.2 1.23 0.017 1.4%
Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Stephen Peterson

Facility Name: City of Roseville Water Treatment Plant

City, State: Roseville, Ca

Calibration Check

0.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1. Standard Preparation - Chlorine Concentrations

1.1.5 Actual Chlorine Concentration: 4.72 (mg/L Cl2

)

1.2.5 Actual Chlorine Concentration: 5.02 (mg/L Cl2

)

1.3.5 Actual Chlorine Concentration: 4.9 (mg/L Cl2

)

1.4.5 Actual Chlorine Concentration: 4.84 (mg/L Cl2

)

1.53 2%

1.47 2%

0.57 14%

0.44 12%
2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Stren

Data:

0.13 0.09 0.06

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.07 0.08 0.11

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Stre

Data:

0.06 0.05 0.07

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.07 0.05 0.08

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength

Data:

0.14 0.1 0.14

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.13 0.12 0.1

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

Data:

0.1 0.1 0.14

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.1 0.11 0.1

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strengt

Data:

1.08 1.02 1.01

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.06 1.03 1

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strengt

Data:

0.99 0.99 1.04
Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.02 1 0.99

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strengt

Data:

1.04 0.96 1

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.01 1.03 0.99

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Streng

Data:

1.07 1.04 1.04

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.06 1.09 1.05

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent 

Data:

0.95 0.91 0.95

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.94 0.89 0.93

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent

Data:

0.94 0.87 0.9

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.91 0.93 0.89

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
 in Drinking Water

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

1.55 3%

1.49 1%

0.55 10%

0.46 8%
DNS Reagent – Low Ionic Strength

0.07

Sample 4 (mg/L F)

DNS2 Reagent – Low Ionic Strength

0.06

Sample 4 (mg/L F)

eagent – High Ionic Strength

0.14

Sample 4 (mg/L F)
eagent – High Ionic Strength

0.12

Sample 4 (mg/L F)

S Reagent – Low Ionic Strength

1.01

Sample 4 (mg/L F)

S2 Reagent – Low Ionic Strength

1.06
Sample 4 (mg/L F)

S Reagent – High Ionic Strength

0.99

Sample 4 (mg/L F)

S2 Reagent – High Ionic Strength

1.05

Sample 4 (mg/L F)
0.92

Sample 4 (mg/L F)

0.89

Sample 4 (mg/L F)
Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Pat Wiese

Facility Name: Hach Company

City, State: Loveland, CO

Calibration Check

0.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1. Standard Preparation - Chlorine Concentrations

1.1.5 Actual Chlorine Concentration: 4.5 (mg/L Cl2

)

1.2.5 Actual Chlorine Concentration: 4.8 (mg/L Cl2

)

1.3.5 Actual Chlorine Concentration: 4.4 (mg/L Cl2

)

1.4.5 Actual Chlorine Concentration: 4.5 (mg/L Cl2

)

1.51 0.7%

1.50 0%

0.56 12%

0.55 10%
2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Stren

Data:

0.10 0.10 0.09

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.09 0.08 0.10

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Stre

Data:

0.06 0.07 0.06

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.05 0.07 0.06

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength

Data:

0.11 0.16 0.15

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.13 0.16 0.14
Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

Data:

0.14 0.13 0.13

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.13 0.13 0.12

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strengt

Data:

1.02 1.02 1.02

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.02 1.02 1.03

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strengt

Data:
1.01 1.00 0.99

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.99 1.00 1.00

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strengt

Data:

1.07 1.07 1.06

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.04 1.05 1.06

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Streng

Data:

1.04 1.02 1.03

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.02 1.03 1.02

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent 

Data:

0.95 0.96 0.96

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.97 0.96 0.97

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent

Data:

0.94 0.94 0.94

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.94 0.95 0.95

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
 in Drinking Water

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

1.51 0.7%

1.49 0.7%

0.55 10%

0.55 10%
DNS Reagent – Low Ionic Strength

0.09

Sample 4 (mg/L F)

DNS2 Reagent – Low Ionic Strength

0.06

Sample 4 (mg/L F)

eagent – High Ionic Strength

0.14

Sample 4 (mg/L F)
eagent – High Ionic Strength

0.13

Sample 4 (mg/L F)

S Reagent – Low Ionic Strength

1.00

Sample 4 (mg/L F)

S2 Reagent – Low Ionic Strength
0.98

Sample 4 (mg/L F)

S Reagent – High Ionic Strength

1.05

Sample 4 (mg/L F)

S2 Reagent – High Ionic Strength

1.01

Sample 4 (mg/L F)
0.96

Sample 4 (mg/L F)

0.95

Sample 4 (mg/L F)
Drinking Water Sample Matrix Validation with Distillation - Loveland, CO

SPADNS Data:

Replicate 1 Replicate 2 Replicate 3 Replicate 4

SPADNS 2 Data:

Replicate 1 Replicate 2 Replicate 3 Replicate 4

0.90 0.98 1.09 1.01

0.92 0.99 1.10 1.02
Hach 10225

Standard Standard

Average Result Deviation Average Result Deviation

Distillation Results 

(mg/L F) 1.01 0.075 1.00 0.079

SM 4500-F D
Datasheet for the Validation of SPADNS 2 Fluoride Reagent in Drinking Water

Name: Cory Sutton

Facility Name: Storm Lake Water Treatment Plant

City, State: Storm Lake, Iowa

Calibration Check

0.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1.5 mg/L F- standard solution:

SPADNS:

Replicate 1 (mg/L F) % Difference

SPADNS 2:

Replicate 1 (mg/L F) % Difference

1. Standard Preparation - Chlorine Concentrations

1.1.5 Actual Chlorine Concentration: 3.4 (mg/L Cl2

)

1.2.5 Actual Chlorine Concentration: 2.8 (mg/L Cl2

)

1.3.5 Actual Chlorine Concentration: 2.6 (mg/L Cl2

)

1.4.5 Actual Chlorine Concentration: 2.1 (mg/L Cl2

)

1.53 2%

1.60 7%

0.59 18%

0.58 16%
2.1 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS Reagent – Low Ionic Stren

Data:

0.15 0.14 0.15

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.19 0.22 0.23

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.2 Method Detection Limit (MDL) Validation Study Results for Liquid SPADNS2 Reagent – Low Ionic Stre

Data:

0.11 0.10 0.04

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.07 0.07 0.09

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.3 Method Detection Limit (MDL) Validation Study for Liquid SPADNS Reagent – High Ionic Strength

Data:

0.11 0.07 0.10

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.09 0.11 0.07
Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

2.4 Method Detection Limit (MDL) Validation Study for Liquid SPADNS2 Reagent – High Ionic Strength

Data:

0.10 0.12 0.10

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.08 0.11 0.13

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.1 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – Low Ionic Strengt

Data:

0.95 0.97 0.95

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.93 0.93 0.94

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.2 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – Low Ionic Strengt

Data:
1.03 0.98 0.96

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.99 0.99 0.98

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.3 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS Reagent – High Ionic Strengt

Data:

0.98 0.97 0.93

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.94 0.96 0.97

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

3.4 Initial Precision and Recovery (IPR) Validation Study for Liquid SPADNS2 Reagent – High Ionic Streng

Data:

1.04 0.99 1.01

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

0.99 1.02 1.01

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
4.1 Drinking Water Sample Matrix Validation Study for Liquid SPADNS Reagent 

Data:

1.21 1.22 1.25

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.25 1.17 1.22

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)

4.2 Drinking Water Sample Matrix Validation Study for Liquid SPADNS2 Reagent

Data:

1.25 1.22 1.22

Sample 1 (mg/L F) Sample 2 (mg/L F) Sample 3 (mg/L F)

1.24 1.24 1.20

Sample 5 (mg/L F) Sample 6 (mg/L F) Sample 7 (mg/L F)
 in Drinking Water

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

Replicate 2 (mg/L F) % Difference

1.53 2%

1.58 5%

0.60 20%

0.57 14%
DNS Reagent – Low Ionic Strength

0.18

Sample 4 (mg/L F)

DNS2 Reagent – Low Ionic Strength

0.10

Sample 4 (mg/L F)

eagent – High Ionic Strength

0.10

Sample 4 (mg/L F)
eagent – High Ionic Strength

0.11

Sample 4 (mg/L F)

S Reagent – Low Ionic Strength

0.98

Sample 4 (mg/L F)

S2 Reagent – Low Ionic Strength
1.01

Sample 4 (mg/L F)

S Reagent – High Ionic Strength

0.96

Sample 4 (mg/L F)

S2 Reagent – High Ionic Strength

0.97

Sample 4 (mg/L F)
1.22

Sample 4 (mg/L F)

1.22

Sample 4 (mg/L F)