### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## GLP10-01-02 : Interi m Repor t 1 8 - Analysi s o fPFBS
. PFHS
. an d PFO S i n Groundwate r Sample s Collecte d a t th e Forme rSludg e Incorporatio n Are a (FSIA ) locate d i n Decatur . A L i n Jun e 201 1 Stud yTitl e Analysi s o fPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e Sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen tfo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Progra m Dat a Requiremen t EPATSC AGoo d Laborator yPractic e Standard s 4 0 CF R Par t79 2 Stud yDirecto r Jaisimh a Kesar i P.E. , DE E Westo n Solutions , Inc . 140 0 Westo n Way Westchester , PA1938 0 Phone:610-701-376 1 Autho r Susa n Wol f 3 M Environmenta l Laborator y Interi m Repor tCompletio n Dat e

Dat e o fsignin g Performin g Laborator y 3 M Environmenta l Healt h an d Safet yOperation s Environmenta l Laborator

y 3 M Center , BId g 260-05-N-1 7 St
. Paul , M N 55144 ProjectIdentificatio n GLP10-01-02-1 8 Tota l Numbe ro fPage s

11 3
Th e testin g reporte d herei n mee t th e requirement s o fISO/IE C 17025-2005 "Genera l Requirement s fo rth e Competenc e o fTestin g an d Calibratio n L^lx>ratories" , i n accordanc e wit h th e A2L ACertificat e #2052.01 . ^ED I Testin g tha t compiie s wit h thi s Internationa l Standar d als o operat e i n accordanc e witt i IS O 9001:2000 . 

## Testin g Cer t #2052.0 1

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### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Thi s pag e has bee n reserve d fo rspecifi ccountr yrequirements .

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



# GL P COMPLIANC E STATEMEN T Repor t Title : Interi m Repor

t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate rSample s Collecte d a

t th e Forme rSludg e Incorporatio n Are a (FSIA ) i n Decatur , A Li n Jun e 201 1 Study : Analysi s ofPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonate (PFHS ) an d Perfluorobutan e Sulfonate (PFBS ) i n Groundwater , Soi l an d Sedimen tfo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Program . Thi s analytica l phas e wa s conducte d i n compliance wit h Toxi cSubstance s Contro l Ac t(TSCA ) Goo d Laborator yPractice (GLP ) Standards , 4 0 CF R 792 , wit h th e exception s liste d below :

•Thes e are environmenta l sample s where there i s n o specifi ctestsubstance , n o specifi ctest syste m an d n o dosin g o

fa testsystem . •Th e reference substance s have no tbee n characterize d unde rth e GLP s an d th e stabilit yunde r storag e condition s a

t th e test sit e have no

tbee n determine d unde rGLPs
.

' - ^ Gar yHof)enstein,Sponso rRepresentative Date

Jaisimh a Kesari , P.E. , DEE , Stud yDirecto rDate

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### GLP10-01-02 ; Interi m Repor t 1 8 /^alysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



# QUALITYASSURANC E STATEMEN T Repor t Title : Interi m Repor

t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Groundwate rSample s Collecte d a

t th e Forme rSludg e Incorporatio n Are a (FSIA ) i n Decatur , A Li n Jun e 201 1 Study : Analysi s ofPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonate (PFHS ) an d Perfluorobutan e Sulfonate (PFBS ) i n Groundwater , Soi l an d Sedimen tfo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Program . Thi s analytica l phas e wa s audite d b yth e 3 M Environmenta l Laborator yQualit yAssurance Uni t(QAU) , as indicate d i n th e followin g table . Th e finding s were reporte d t o th e principa l investigato r(P.I.) , laborator ymanagemen t an d stud ydirector .
Inspectio n Date s 8/18/11-8/19/1 1 Phas e Dat a / Repor t Dat e Reporte d t o

Testin g Facilit y Managemen t 8/25/1 1 Stud yDirecto r 8/25/1 1 

# n ^ L 

# '^-l- K QA U Representaiv e Date

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



# TABL E O F CONTENT S GL P Compliance statemen t 3 Qualit yAssuranc e Statemen t 4 Tabl e ofContents 5 List o fTable s 6 1 Stud yInfomriatio n 8 2 Summar

y9 3 Introductio n 1 1 4 Test & Contro l Substance s 1 1 5 Reference Substance s 1 2 6 Test Syste m 1 3 7 Metho d Summar

y1 3 7. 1 Method s 1 3 7. 2 Sampl e Collectio n 1 3 7. 3 Sampl e Preparatio n 1 3 7. 4 Analysi s 1 4 8 Analytica l Results 1 5 8. 1 Calibratio n 1 5 8. 2 Syste m Suitabilit y1 5 8. 3 Limi t ofQuantitatio n (LOQ) 1 6 8. 4 Continuin g Calibratio n 1 6 8. 5 Blank

s 1 6 8. 6 La b Contro l Spike s (LCSs ) 1 6 8. 7 . Analytica l Metho d Uncertaint y2 0 8. 9 Fiel d Matri xSpike s (FMS ) 2 1 9 Data Summar

yan d Discussio n 2 1

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## 1 0 Conclusio n 3 9 1 1 Data/Sampl e Retentio n 3 9 1 2 Attachments 3 9 1 3 Signature s 4 0 LIS TO F TABLE S Tabl e 1 . Summarize d PFBS , PFHS , an d PFO S Results (FSI AGroundwater , Jun e 2011 ) 1 0 Tabl e 2 . Sampl e Descriptio n Ke yCod e 1 3 Tabl e 3 . Instrumen tParameter s 1 4 Tabl e 4 . Liqui d Chromatograph yCondition s 1 4 Tabl e 5 . Mass Transition s 1 5 Tabl e 6 . Limi tofQuantitatio n (LOQ ) 1 6 Tabl e 7 . Laborator

yContro l Spik

e Recover

y1 8 Tabl e 8 . Analytica l Uncertaint y2 0 Tabl e 9 . Fiel d Matri xSpik e Level s 2 1 Tabl e 10 . DA LGW130 R 110630 2 3 Tabl e 11 . DA LGW130 S 110630 2 3 Tabl e 12 . DA LGW 1 SO

L110630 2 4 Tabl e 13 . DA LG W131 R 110622 2 4 Tabl e 14 . DA LGW131 S 110622 2 5 Tabl e 15 . DALGW131 L110622 2 5 Tabl e 16 . DA LGW133 R 116030 2 6 Tabl e 17 . DA LGW133 S 110630 2 6 Tabl e 18 . DALGW133L11063 0 2 7 Tabl e 19 . DALGW134R11062 8 2 7 Tabl e 20 . DALGW134 S 11062 8 2 8 Tabl e 21 . DALGW134L11062 8 2 8

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 22 . DALGW135R11062 8 2 9 Tabl e 23 . DA LGW135 S 11062 8 2 9 Tabl e 24 . DALGW135L11062 8 30 Tabl e 25 . DA LGW136 S 110630 3 0 Tabl e 26 . DALGW136L11063 0 3 1 Tabl e 27 . DA LGW137 S 11062 4 3 1 Tabl e 28 . DA LGW137 L11062 4 32 Tabl e 29 . DA LGW138 S 110630 32 Tabl e 30 . DALGW138L11063 0 33 Tabl e 31 . DA LGW138 R 110630 3 3 Table32 . DALGWCW26 C 110622 34 Table33 . DALGWCW26L11062 2 34 Table34 . DALG W140 R 110622 3 5 Table35 . DALG W141 R 110630 35 Table36 . DALG W142 R 110622 36 Table37 . DALG W143 R 110622 3 6 Table38 . DALG W144 S 11062 8 3 7 Tabl e 39 . Tri p Blan k

1 37 Tabl e 40 . Tri p Blan k

2 38 Tabl e 41 . Rinseate Blank s 3 8

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### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## ii.i^ ' 

## Sponso r 3 M Compan y Sponso rRepresentativ e Gar yHohenstei n 3 M EH S Operation s 3 M Buildin g 224-5W-0 3 Sain t Paul , M N 55144-100 0 Phone:(651)737-357 0 Stud yDirecto r Jaisimh a Kesari , P.E. , DE E Westo n Solutions , Inc . Westchester , P

A1938 0 Phone:(610)701-376 1 Fax:(610)701-740 1 j.kesari@westonsolutions.co m Stud yLocatio n Testin g Facilit y 3 M EH S Operation s 3 M Environmenta l Laborator y Buildin g 260-5N-1 7 St
. Paul , M N 55144 Stud yPersonne l Willia m K. Reagen , Ph.D. , 3 M Laborator yManage r Clesto n Lange , Ph.D. , Principa l Analytica l Investigator , (clanqe@mmm.com) : phon e (651)-733-986 0 Susa n Wolf , 3 M Analyst Chelsi e Grochow , Analys t Kevi n Eich , Analys t Kell yUkes , Analys t Jonatha n Steege , Analys t Stud yDate s StudyInitiation : Marc h 8,201 0 Interi m 1 8 Experimenta l Termination : August3,201 0 Interi m Repor

t Completion : Date ofInteri m Repor tSignin g Locatio n o fArchive s Al l origina l rawdat a an d th e analytica l repor thave bee n archive d a tth e 3 M Environmenta l Laborator yaccordin g t o 4 0 CF R Par t792 . Th e tes tsubstanc e an d analytica l referenc e standard reserv e sample s ar e archive d a t th e 3 M Environmenta l Laborator yaccordin g t o 4 0 CF R Par t79 2

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### GLP10-01-02 ; interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1



# g mmrnM ^ Th e 3 M Environmenta l Laboratoryreceive d groundwate rsample s fro m well s locate d a t th e Forme r Sludg e Incorporatio n Are a (FSIA ) i n Decatur , AL , representin g twenty-eigh t differen t samplin g locations . Atota l o fon e hundre d fifty-fou rsampl e bottle s wer e receive d a t th e 3 M Environmenta l Laboratoryfo rperfluorooctan e sulfonat e (PFOS) , perfluorohexan e sulfonat e (PFHS ) an d perfluorobutan e sulfonat e (PFBS) , an d include d duplicat e groundwate rsample s fro m eac h samplin g location . Sample s als o include d a t leas t tw o fiel d matri xspik e (FMS ) sample s fo reac h location , tw o tri p blan k set s containin g Milli-Q TM wate ran d appropriat e tri p blan k spikes , an d tw o equipmen t rinseate blanks . Th e equipmen t rinseate blank s di d no t hav e FM S sample s prepare d fo rdetenninatio n o f PFBS , PFHS , an d PFO S recovery . Th e groundwate rsamples , tri p blank s an d tw o equipmen t rinseate blank s associate d wit h GLP10-01 - 02-1 8 wer e receive d fro m Westo n personne l o n Jul y8 , 2011 . Ai l o fth e sample s wer e prepare d an d analyze d fo rPFBS , PFHS , an d PFO S followin g 3 M Environmenta l LaboratoryMetho d ETS-8-044. 0 an d conducte d unde r3 M projec t GLP-10-01-02-18
. Man yo fth e groundwate rsample s require d dilutio n t o attai n PFBS , PFHS , and/o rPFO S concentration s withi n th e rang e o fth e curve , i n som e instance s u p t o 100-fol d dilutio n wer e required . Th e averag e measured PFBS , PFHS , an d PFO S concentration s ar e summarize d i n Tabl e 1 . Th e equipmen t rinseate fro m DA LG W1 SO Lan d th e tri p blan k sampl e wer e belo wth e lowe rlimi t o fquantitatio n (LLOQ ) fo rPFBS , PFHS , an d PFOS , indicatin g adequat e contro l o fsampl e contaminatio n durin g shippin g an d sampl e collections . Th e equipmen t rinseate fro m sampl e DA LG W133 S fo rPFO S wa s jus t abov e th e LLO Q a t 0.047 8 ng/mL , whil e PFB S an d PFH S wer e belo wth e lowe rlimi t o fquantitatio n (LLOQ)
. Th e PFB S concentratio n result s fo ral l groundwate rsample s range d fro m 0.039 1 ng/m Lt o 74. 3 ng/mL
. Th e PFH S concentratio n result s fo rai l groundwate rsample s range d fro m 0.080 6 ng/m Lt o 84 4 ng/mL
. Th e PFO S concentratio n result s fo ral l groundwate rsample s range d fro m 0.48 3 ng/m Lt o 151 0 ng/mL
. Th e analytica l metho d uncertaintie s associate d wit h th e reporte d result s are : PFBS + 1 1 % , PFH S + 14 % an d PFO S + 36% . Fo rsom e samples , th e metho d uncertaint ywa s expande d fo rPFOS
.

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 1 . Summarize d PFBS , PFHS , an d PFO S Result s (FSI AGroundwater , Jun e 2011)
. Samplin g Locatio n 

## DA LGW130 R 110630 DALG W130 8 11063 0 DA LGW 1 SO

L110630 DA LG W131 R 11062 2 DA LGW131 S 11062 2 DA LG W131L11062 2 DA LGW133 R 110630 DALG W133 8 110630 DA LGW133 L110630 DA LGW134 R 11062 8 DALG W134 8 11062 8 DALGW134L11062 8 DA LGW135 R 11062 8 DALG W135 8 11062 8 DA LGW135 L11062 8 DALG W136 8 110630 DALGW136L11063 0 DALG W137 8 11062 4 DA LGW137 L110624 DA LGW138 R 110630 DALG W138 8 11063 0 DALGW138L11063 0 DA LGWCW26 C 110622 DA LGWCW26 L110622 DA LGW140 R 11062 2 DA LG W141 R 110630 DA LGW142 R 11062 2 DA LGW143 R 11062 2 DALG W144 3 11062 8 Tri p Blank

s (Milli-Q TM Water

) DA LGW130LR B 110630 DALG W133 8 RB11063 0 PFB S Avg . Cone
. (ng/mL ) ±RP D 16. 3 ±1.8 % 35 3 ±00 % 1.6 7 ±1.8 % 54. 9 ±3.5 % 089 4 ±4.6 % 3.42 ±1.2 % 4.7 9 ±3.8 % 9.0 8 ±1.4 % 6.6 7 ±0.45 % 51. 4 ±1.4 % 0.25 8 ±2.3 % 11. 8 ±6.8 % 74. 3 ±1.6 % 1.2 1 ±0.83 % 9.6 9 ±0.83 % 3.6 9 ±1.1 % 2.33 ±0.43 % 0.10 7 ±2.8 % 0.082 6 ±1.9 % 8.0 1 ±0.0 % 4.5 7 ±0.66 % 0039 1 ±2.8 % 8.2 3 ±073 % 13. 5 ±0.74 % 1.59 ±1.3 % 0.043 1 ±2.8 % 1.6 9 ±0.0 % 1.6 6 ±2.4 % 1.6 6 ±4.2 % <0.025 0 <0.025 0 <0.025 0 PFH S

Avg . Cone
. (ng/mL ) ±RP D 12 5 ±0.0 % 29. 8 ±0.34 % 13. 6 ±2.2 % 30 4 ±4.6 % 5.0 7 ±7.5 % 21. 6 ±1.9 % 33 1 ±3.0 % 67. 2 ±0.74 % 46. 9 ±1.7 % 48 3 ±0.62 % 3.0 9 ±2.3 % 11 1 ±13 % 84 4 ±3.7 % 8.0 6 ±2.2 % 71. 0 ±2.5 % 25. 8 ±0.78 % 16. 0 ±1.3 % 0.080 6 ±2.0 % 0.10 7 ±094 % 39. 2 ±0.26 % 23. 2 ±0.0 % 011 3 ±0.89 % 67. 8 ±1.6 % 10 5 ±1.0 % 17. 8 ±1.1 % 0.29 6 ±1.7 % 10. 2 ±3.0 % 6.0 6 ±3.8 % 7.1 4 ±3.9 % <0.025 0 <0.025 0 <0.025 0 PFO S

Avg . Cone
. (ng/mL ) ±RP D 45 0 ±4.4 % 97. 6 ±5.0 % 63. 7 ±5.3 % 151 0 ±3.3 % 5.3 7 ±2.1 % 32. 4 ±1.5 % < " 42. 6 ±7.3 % 19 5 ±1.0 % 14 6 ±4.8 % <^

' 106 0 ±13 % 13. 3 ±0.0 % 493±22%<^ ' 96 9 ±11 % NR<^ > 57. 6 ±7.3 % <^

' 11 6 ±0.87 % 69. 0 ±10%'^ ' 0.48 3 ±6.4 % 1.9 6 ±2.6 % 34 7 ±0.58 % 19 9 ±4.5 % 0.85 1 ±2.6 % 39 8 ±00 % 93 4 ±4.9 % 12 8 ±13 % 3.0 0 ±3.0 % 85. 5 ±6.8 % 23. 8 ±3.4 % 29. 0 ±38 % '^ ' <0.10 0 <0.040 0 0.047 8 TTie analytica l metho d uncertaintie s associate d wit h th e reporte d results are : PFB S ±1 1 % , PFH S ±14% , an d PFO S ±36% . (1 ) Th e analytica l uncertaint yhas bee n adjuste d fo rPFO S base d o n FIVI S recover yfo rDA LG W131 Lt o ±39% , D/M
. G W135 L to±37% , DALGW136Lto±41% , an d DA LG W144S t o ±37% . (2 ) Th e lowfiel d matri xspik e fo rPFO S had a recover ythat >200% , whil e th e hig h field matri xspike me t metho d acceptance criteria . (3 ) Th e RP D di d notmeet metho d acceptance criteri a ofs20% . (4) N R = No

t Reported ; Recover

yofth e onl yappropriate fiel d matri xspik e was >200% . Se e sectio n 9 ofth e repor t fo radditiona l information
.
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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Thi s analytica l stud ywa s conducte d as par tofth e Phas e 3 Environmenta l Monitorin g an d Assessmen t Progra m fo rth e 3 M facilitylocate d i n Decatur , Alabama . Th e objective ofth e overal l progra m i s t o gai n informatio n regardin g concentration s ofperfluorooctan e sulfonat e (PFOS) , perfluorohexan e sulfonate (PFHS ) an d perfluorobutan e sulfonat e (PFBS) , i n variou s environmenta l medi a suc h as groundwater , soil s an d sediments that are associate d wit h an d nea rth e Decatu rfacility. Thi s analytica l stud ywas conducte d t o analyz e groundwate rsample s collecte d fro m variou s well s locate d a tth e Forme rSludg e Incorporatio n Are a (FSIA

) i n Decatur , A Lfo rPFBS , PFHS , an d PFO S i n a n effor tt o characteriz e on sit e groundwate rconditions . Th e 3 M Environmenta l Laborator yprepare d sampl e container s (25 0 m Lhigh-densit

ypolyethylen e bottles ) whic h were shippe d t o Decatur , A LWesto n personne l prio rt o fiel d sampling . Sampl e container s fo reac h samplin g locatio n include d a fiel d sample , field sampl e duplicate , an d a minimu m of two fiel d matri xspike samples . Eac h empt ycontaine rwa s mari<e

d wit h a "fil l t o here " lin e t o produce a fina l sampl e volum e of20 0 mL . Container s designate d fo rfiel d matri xsample s were fortifie d wit h a n appropriat e matri xspike solutio n containin g PFB S (linear) , PFH S (linear) , an d PFO S (linear ) prio rt o bein g sen t t o th e fiel d fo rsampl e collection . Se e sectio n 8. 8 ofth e repor tfo rfiel d matri xspike levels . Sampl e bottle s fo rselec

t samplin g location s were fortifie d wit h intema l standar d prio rt o sampl e collectio n t o ai d i n sampl e analysis . Thes e include d samplin g locations : DA LG W134S , DA LG W135S , DA LG W136R , DA LG W137R , DA LG W137S , DA LG W137L , DA LG W138L , Tri p Blan k samples , an d equipmen t rinseate blanks . Where applicable , intema l standard s were use d t o ai d i n th e data qualit yobjectives . Sample s were prepare d an d analyze d accordin g t o th e procedur e define d i n 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 "Detenninatio n ofPerfluorinate d Compound s I n Wate rb yHig h Perfonnance Liqui d Chromatography/Mas s Spectrometr yDirec tInjectio n Analysis" . Tabl e 1 summarize s th e averag e PFBS , PFHS , an d PFO S concentration s fo rth e duplicat e sample s collected , th e tri p blank , an d th e aqueou s equipmen trinseate blan k samples . Table s 10-4 1 summariz e th e individua l sampl e results an d th e associate d fiel d matri xspik e recoveries . Al l results fo rth e qualit y contro l sample s prepare d an d analyze d wit h th e sample s are reporte d an d discusse d elsewhere i n thi s repor t

There wa s no

t a test substance o rcontro l substance s i n th e classi csens e ofa GL P study . Thi s stud y wa s purel yanalytica l i n nature .

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### GLP10-01-02 ; Interi m Repor t 1 8 /^alysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Referenc e Substanc e Chemica l Nam e Chemica l Formul a Identifie r Us e Source Expiratio n Date Storag e Condition s Chemica l Lo

t Numbe r TC R Numbe r Physica l Descriptio n Purit y PFB S (predominantl ylinear ) Perfluorobutan e sulfonate C4F9SO3 " Potassiu m Sal t Target Analyt e Reference Standar d 3 M 1/10/201 7 Froze n 41-2600-8442- 5 TCR-12 1 Whit e Powde r 96.7 % PFH S (lineal ) Perfluorohexan e sulfonate CeFisSOa ' Sodiu m Sal t Target Analyt e Reference Standar d Wellingto n 4/2/201 3 Froze n LPFHxSAMO

S TCR08-001 8 Crystallin e 98 %

Chemica l Nam e Chemica l Formul a Identifie r Use Source Expiratio n Date Storag e Condition s Chemica l Lo

t Numbe r TC R Numbe r Physica l Descriptio n Purit y PFOS (linear ) Perfluorooctan e sulfonate CsFiySOs ' Potassiu m Sal t CAS #2795-39- 3 Target Analyt e Reference Standar d Wellingto n 10/18/201 3 Froze n LPFO8KBM0 6 TCR08-000 1 Crystallin e 98 % PFOS

(linea r+ branclied ) Perfluorooctan e sulfonate C8F17SO3" Potassiu m Sal t CAS #2795-39- 3 LC S Reference Standar

d 3 M 12/14/201 6 Froze n 17 1 TCR-69 6 Whit e Powde r 86.4 % "CB-PFO S 

## Sodiu m Periluorooctanesulfonate "CsFiySOaNa " CAS#MPFC-C-051 1 Intema l Standar

d Wellingto n 05/25/201 4 Froze n 09231 0 TCR11-001 6 Liqui d 5 |jg/m L<^

' ( C ) periluoroalkylsulfonate s an d on e mass-labele d ("C ) perfluoro-1-octanesulfbnamide
.

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Th e tes t system s fo rthi s stud yare groundwate rsample s collecte d fro m well s locate d i n Decatur , A Lb y Westo n Solutions , Inc . personnel . Sample s fo rthi s stud yare "rea l worid " samples , no tdose d wit h a specifi clot oftes t substance . Tabl e 2 . Sampl e Descriptio n Ke yCode
. strin g Numbe r Exampl e 1 2 3 4
5 6 strin g Descripto r Exampl e DA LGW131 R 011062 2 Samplin g Locatio n Wel l I D Samplin g Poin t Wel l Leve l Samplin g Dat e Sampl e Typ e DA L= Decatur , Alabam a GW = Groundwate r Example : 13 1 R = Residu m shallo wwater-bearin g zon e L= Bedroc k

water-bearin g zon e S = Epikars t middl e water-bearin g zon e 11062 2 = June22,201 1 0=primarysampl e DB=duplicat e sampl e L S = lo wspik e M S = mi d spik e H S = hig h spik e



# 7. 1 Method s Analysi s fo ral l analyte s wa s complete d followin g 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 "Determinatio n ofPerfluorinate d Compound s I n Wate rb yHig h Perfonnance Liqui d Chromatography/Mas s Spectrometr yDirec tInjectio n Analysis" .
-T M , 

# 7. 2 Sampl e Collectio n Sample s were collecte d i n 25 0 m LNalgene'" " (high-densit ypolyethylene ) bottle s prepare d a tth e 3 M Environmenta l Laboratory . Sampl e bottle s associate d wit h GLP10-01-02-1 8 were retume d t o th e laborator ya t ambien t condition s o n Jul y8 , 2011 . Sample s were store d refrigerate d a tth e laborator

y afte rreceipt . Aset oflaborator yprepare d Tri p Blan k

an d Tri p Blan k

fiel d matri xspike s were sen twit h eac h coole rofsampl e collectio n bottles . 7. 3 Sampl e Preparatio n Sample s were prepare d b yremovin g a n aliquotofth e wel l mixe d sampl e an d placin g i ti n a n autovia l fo r analysis . Sample s that require d dilutio n were prepare d b ydilution s of1 m Lsampl e wit h 9 mL s o fMill i Q wate r (dilutio n 1:10)
. Sample s requirin g a 1:100 dilutio n were prepare d b yaddin g 0. 1 m Lsampl e t o 9. 9 mLs ofMillJQ water . Sample s associate d wit h samplin g location s DA LG W143 R an d DA LG W144 S were spike d wit h intema l standar

d b yaddin g a n aliquot ofintema l standar d stoc k solutio n into 1 0 mL

s ofsample . Interna l standar d wa s adde d t o ai d o n th e quanitatio n ofth e samples .
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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



## 7. 4 Analysi s Al l studysample s an d qualit ycontro l sample s were analyze d fo rPFBS , PFHS , an d PFO S usin g hig h perfomnanc

e liqui d chromatography/tande m mass spectrometr y(HPLC/MS/MS)
. Detaile d instmmen t parameters , th e liqui d chromatograph ygradien tprogram , an d th e specifi cmas s transition s analyze d are describe d i n th e ra

wdata har

d copie s place d i n th e fina l data packet , an d are briefl ydescribe d below . Tabl e 3 . Instrumen t Parameters . Instrumen tNam e Analytica l Metho d Followe d Analysi s Dat e Liqui d Chromatograp h Guar d colum n Analytica l colum n Injectio n Volum e lUlas s Spectromete r Io n Sourc e Electrod e Polarity Softwar e ETSKir1 < ETS-8-044. 0 7/20/1 1 - PFBS , PFHS , an d PFO S

Agilen t 1200 Pris m R P (2. 1 m m X5 0 mm) , 5 n Betasi l 01 8 (2. 1 m m X100 mm) , 5\ i

2 o r1 0 ^ L Applie d Biosystem s PP

\ 550 0 Turtx) Spra

y Turtx) io n electrod e Negativ e Analyst 1.5. 2 ETS Kir k ETS-8-044. 0 7/29/ 1 lan d 8/2/11-PFO S Agilen t 1200 Betasi l 01 8 (4. 6 m m X100 mm) , 5 n Betasi l 01 8 (4. 6 m m X100 mm) , 5 n

2 5 n L Applie d Biosystem s AP I 550 0 Turb o Spra

y Turtx) io n electrod e Negativ e Analyst 1.5. 2 

## Tabl e 4 . Liqui d Chromatograph yConditions
. Ste p Numbe r Tota l Tim e

(min ) Flo wRat e

(fiUmin ) Percen t > 4 ( 2 m M ammoniu m acetate ) Percent S (Methanol ) ETS-8-044. 0 Analyze d 7/20/1 1 0 1 2 3 4 5 0

2. 0 14. 5 15. 5 16. 5 20. 0 30 0

30 0 30 0 30 0 30 0 30 0 97. 0 97. 0 5. 0 5. 0 97. 0 97. 0 3. 0 3. 0 95. 0 95. 0 3. 0 3. 0 ETS-8-044. 0 Analyze d 7/29/1 1 an d 8/2/1 1 0 1 2 3 4 • 5 0

2. 0 14. 5 15. 5 16. 5 20. 0 75 0

75 0 75 0 75 0 75 0 75 0 97. 0 97. 0 5. 0 5. 0 97. 0 97. 0 3. 0 3. 0 95. 0 95. 0 3. 0 3. 0

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### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



## Tabl e 5 . Mas s Transitions .

Analyt e PFB S PFH S PFO S Mas s Trarjsitio n

Q1/Q 3 299/8 0 299/9 9 399/8 0 399/9 9 499/8 0 499/9 9 499/13 0 Referenc e

Materia l structur e Linea r Linea r Linea r Intema l Standar d

N A N A t^Cel-PFOS'^ ' Mas s Transitio n Q1/Q 3 N A N A 507/8 0 Dwel l tim e was 3 0 mse cfo reac h transition . Th e individua l transition s were summe d t o produce a "tota l

io n chromatogram " (TIC) , whic h was use d forquantitation . (1 ) Intema l standar

d was onl yuse d fo rPFO S fo rsamplin g location s DA LG W1358 , DA LG W1378 , DA LG W137L , DA LG W138L , DA LG W144 R an d DA LG W1438 . 

## 8 Analytica l Result s 8. 1 Calibratio n Sample s were analyze d against a n externa l standar d calibratio n curve . Calibratio n standard s were prepare d byspikin g know n amounts ofth e stoc k solutio n containin g th e targetanalyte s into prepare d i n Mill i Q water . Atota l offiftee n spike d standard s rangin g fro m 0.02 5 ng/m Lt o 10 0 ng/m L(nominal ) wer e analyzed . Aquadratic , 1/ xweighted , calibratio n curve o fth e pea k are a counts was use d t o fitth e data fo reac h analyte . Th e data were no t force d throug h zer

o durin g th e fitting process . Calculatin g th e standar d concentration s usin g th e pea k are a confirme d accurac

yofeac h curve point . Fo rsamplin g location s DA LG W135S , DA LG W137S , DA LG W137L , DA LG W138L , DA LG W143R , DA LG W144 R an d rinseate blank s fo rPFOS : Sample s were analyze d usin g a stabl e isotop e intema l standar d calibratio n curve . Calibratio n standard s were prepare d byspikin g know n amounts ofth e stoc k solutio n containin g th e targe t analyte s into a laboratory-prepare d syntheti cgroundwate rcontainin g calciu m an d magnesium . Aseparat e intema l standar d spikin g solutio n wa s prepare d an d a n aliquot was adde d a

t th e sam e leve l t o al l calibratio n standard s an d laborator ycontro l sample s a

ta nomina l concentratio n of1 ng/mL
. Acalibratio n curve rangin g fro m approximatel y0.02 5 ng/m Lt o 100 ng/m L was prepared . Aquadratic , 1/ xweighted , calibratio n curve ofth e pea k are a counts wa s use d t o fit th e data fo reac h analyte . Th e dat a were no tforce d throug h zer

o durin g th e fitting process . Calculatin g th e standar d concentration s usin g th e pea k are a ratio s an d th e resultan tcalibratio n curve confirme d accurac yofeac h curve point . Eac h curve poin t wa s quantitate d usin g th e overal l calibratio n curve an d reviewe d fo raccuracy . Method calibratio n accurac

yrequirements o

f100±25 % (100±30 % fo rth e lowestcurve point) were metfo ral l analytes . Th e con-elatio n coefficien t (r) wa s greate rtha n 0.995 fo rPFBS , PFHS , an d PFOS
. 8. 2 Syste m Suitabilit y Acalibratio n standar

d wa s analyze d fou rtime s a tth e beginnin g o feac h analytica l sequence t o demonstrate overal l syste m suitability . Th e acceptance criteri a ofles s tha n o requa l t o 5 % relative standar d deviatio n (RSD ) fo rpea k are a an d retentio n tim e criteri a ofles s tha n o requa l t o 2 % RS D was met fo rPFBS , PFHS , an d PFO S wit h th e exceptio n ofth e externa l calibratio n o fth e syntheti cwate r curve whic h had a n are a coun t RS D of9.3% . Othe rQC item s were use d t o asses s data accurac y

Pag e 1 5 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## includin g calibratio n curv e poin t accuracy , curv e fit criteria , CCVs , an d LCS , al l meetin g metho d acceptanc e criteria . Ametho d deviatio n i s include d wit h th e rawdata . 

# 8. 3 Limi t o fQuantitatio n (LOQ ) Th e LO Q fo rthi s analysi s i s th e lowes t non-zer o calibratio n standar d i n th e curv e tha t meet s linearity an d accuracyrequirement s an d fo rwhic h th e are a count s o rare a rati o ar e a t leas t twic e thos e o fth e appropriat e blanks . Th e LO Q fo ral l analyte s ca n b e foun d i n Tabl e 6 . Tabl e 6 . Limi t o fQuantit a tio n (LOQ)
. Analysi s Dat e 7/20/1 1 Analysis externa l calibratio n

7/29/1 1 Analysis externa l calibratio n 7/29/1 1 Analysis interna l standar

d calibratio n 8/2/1 1 Analysi s - interna l standar

d calibratio n Dilutio n 1 5 1 0 5 0 100 1 1 0 100 1 1 PFB S

LOQ , ng/m L 0.0250 0.125 0.250 1.2 5 2.5 0 N A N A N A N A NA PFH S

LOQ , ng/m L 0.0250 0.12 5 0.25 0 1.2 5 2.5 0 N A N A N A N A NA PFO S

LOQ , ng/m L 0.100 0.500 1.0 0 5.00 10. 0 1.0 0 10. 0 10 0 0.100 0.040 0 N A= No t Applicabl e 

# 8. 4 Continuin g Calibratio n Durin g th e cours e o feac h analytica l sequence , continuin g calibratio n verificatio n sample s (CCVs ) wer e analyze d t o confir m tha t th e instrumen t respons e an d th e initia l calibratio n curv e wer e stil l i n control . Al l CCV s me t metho d criteri a o f100 % ±25 % fo rPFBS , PFHS , an d PFO S excep t fo rth e externa l calibratio n o fth e syntheti cwate rcurv e wher e tw o CCV s ha d recoverie s outsid e metho d acceptanc e criteri a o f100 % ±25 % fo rPFOS
. Th e tw o non-complian t CCV s di d no t bracke t an ysample s reporte d herein . 

# 8. 5 Blank s Tw o type s o fblank s wer e prepare d an d analyze d wit h th e samples : metho d blank s an d field/trip blanks . Metho d blan k

result s wer e reviewe d an d use d t o evaluat e metho d perfonnanc e an d t o determin e th e LO Q fo reac h analyt e 

# 8. 6 La b Contro l Spike s (LCSs ) Low , mid , an d high-leve l la b contro l spike s wer e prepare d an d analyze d i n triplicat e wit h eac h preparatio n set . LCS s wer e prepare d b yspikin g know n amount s o fth e analyt e int o Mill i Q wate ro r syntheti cgroundwate rt o produc e th e desire d concentration . Th e spike d wate rsample s wer e the n prepare d an d analyze d i n th e sam e manne ra s th e samples . Th e metho d acceptanc e criteria , averag e o fLC S a t eac h leve l shoul d b e withi n 100 % ±20 % wit h a n RS D <20% . 7/20/1 1 Analysis : Al l LCS s me t acceptanc e criteri a fo rPFBS , PFHS , an d PFOS
.

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## 7/29/1 1 Analysi s - intema l calibration : Th e lo wsetofLC S sample s fo rPFO S had a n averag e recover y of126% . Th e mi d an d hig h sets ofLC S sample s metacceptance criteri a fo rPFOS
. 7/29/1 1 Analysi s - externa l calibration : Th e lo wsetofLC S sample s fo rPFO S were spike d belowth e LOQ
. Th e mi d an d hig h sets o fLC S sample s metacceptance criteri a fo rPFOS
. 8/2/1 1 Analysi s -Al l LCS s me t acceptance criteri a fo rPFOS
. As th e reference materia l use d fo rquantitafio n o fPFO S i s predominantl ylinear , an d th e PFO S presen t i n th e wate rsample s are comprise d o fbot h linea ran d branche d isomers , additiona l LC S sample s of PFO S (linea ran d branched ) were prepare d a tthre e concentration s i n triplicat e t o evaluat e th e potentia l fo ranalytica l bias . 7/20/1 1 Analysis : Al l linea ran d branche d PFO S LCS s metacceptance criteria . 7/29/1 1 Analysi s - intema l calibration : Th e lo wseto fLC S sample s (linea ran d branched ) fo rPFO S had a n averag e recover

yof136 % an d th e hig h sethad a n averag e recover yof122% . Th e mi d setofLC S sample s met acceptance criteri a fo rPFOS
. 7/29/1 1 Analysi s - externa l calibration : Th e lo wsetofLC S sample s (linea ran d branched ) fo rPFO S were spike d belowth e LOQ
. Th e mi d an d hig h sets ofLC S sample s me tacceptance criteri a fo rPFOS
. 8/2/1 1 Analysis : Th e low , mid , an d hig h linea ran d branche d LCS s had averag e recoverie s of129% , 122% , an d 122% . Th e followin g calculation s were use d t o generat e data i n Tabl e 7 fo rlaborator ycontro l spikes .

, „ „ _. _

, Calculate d Concentratio n , . .„„ , LC S Percen tRecover

y= * 100 % Spike Concentratio n LCS % RS D = standard deviatio n LC S replicate s .. , ^^y ^ averag e LC S recover

y

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



## Tabl e 7 . Laborator yContro l Spik e Recovery . ETS-8-044. 0 Analyze d 7/20/1 1 Externa l standar d calibration La b I D LCS-110714- 1 LCS-110714- 2 LC8-110714- 3 Averag e ±%RS D LCS-110714- 4 LC8-110714- 5 LC8-110714- 6 Averag e ±%RS D LC8-110714- 7 LCS-110714- 8 LCS-110714- 9 Averag e ±%RS D PFB S

Spike d Concentsdo n (ng/mL ) 0.49 8 0.49 8 0.49 8 Calculate d

Concentratio n (ng/mL ) 0.50 7 0.51 4 0.51 4 VoRecover

y 102 103 10 3 103 ±0.56 % 4.9 8 4.9 8 4.9 8 5.1 4

5.1 4 5.1 3 10 3

103 10 3 103 % ±0.0 % 29. 9 29. 9 29. 9 29. 9

29. 0 29. 5 10 0

96. 8 98. 8 98.5 % ±1.6 % PFH S Spike d Concentratio n (ng/mL ) 0.49 8 0.49 8 0.49 8 Calculate d

Concentratio n (ng/mL ) 0.52 4 0.52 6 0.53 7 VoRecover y 10 5 10 6 10 8 106 % ±1.4 % 4.9 8 4.9 8 4.9 8 5.2 1

5.3 0 5.2 0 10 5

10 6 104 105 % ±1.0 % 29. 9 29. 9 29. 9 30. 1

28. 6 29. 8 10 1

95. 6 99. 7 98.8 % ±2.9 % ETS-8-044. 0 Analyze d 7/20/1 1 Externa l standar d calibration La b I D LCS-110714- 1 LC8-110714- 2 LC8-110714- 3 Averag e ±%RS D LC8-110714- 4 LC8-110714- 5 LCS-110714- 6 Averag e ±%RS D LCS-110714- 7 LCS-110714- 8 LC8-110714- 9 Averag e ±%RS D PFO S (linear)

Spike d Concentratio n (ng/mL ) 0.49 8 0.49 8 0.49 8 Calculate d

Concentratio n (ng/mL ) 0.42 7 0.43 2 0.44 8 VoRecover

y 85. 8 86. 8 89. 9 87.5 % ±2.4 % 4.9 8 4.9 8 4.9 8 4.9 3

4.9 2 4.7 2 98. 9

98. 8 94. 7 97.5 % ±2.5 % 29. 9 29. 9 29. 9 30. 5

28. 5 29. 6 102

95. 2 98. 9 98.7 % ±3.4 % La b I D LC8-110714-1 0 LC8-110714-1 1 LCS-110714-1 2 Averag e ±%RS D LC8-110714-1 3 LCS-110714-1 4 LC8-110714-1 5 Averag e ±%RS D LC8-110714-1 6 LC8-110714-1 7 LCS-110714-1 8 Averag e ±%RS D PFO S (linear+branclied )

Spike d Concentratio n (ng/mL ) 0.49 5 0.49 5 0.49 5 Calculate d

Concentratio n (ng/mL ) 0.46 9 0.46 0 0.43 7 VoRecover

y 94. 7 92. 9 88. 3 92.0 % ±3.6 % 4.9 6 4.9 6 4.9 6 4.1 4

4.3 3 4.4 6 83. 5

87. 3 89. 9 86.9 % ±3.7 % 29. 8 29. 8 29. 8 29. 4

29. 3 28. 5 98. 6

98. 3 95. 6 97.5 % ±1.7 %

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 7 continued . Laborator yContro l Spik e Recovery . ETS-8-044. 0 Analyze d 7/29/1 1 Intemal standar

d calibration La b I D LC8-110726- 1 LC8-110726- 2 LCS-110726- 3 Averag e ±%RS D LC8-110726- 4 LC8-110726- 5 LCS-110726- 6 Averag e ±%RS D LC8-110726- 7 LC8-110726- 8 LC8-110726- 9 Averag e ±%RS D PFO S (linear)

Spike d Concentratio n (ng/mL ) 0.19 9 0.19 9 0.19 9 Calculate d

Concentratio n (ng/mL ) 0.25 7 0.24 8 0.25 0 VoRecover

y 12 9 12 5 12 5 126 % ±1.8%*^ ' 1.99 1.99 1.99 2.1 1

2.0 0 2.1 1 10 6

10 1 10 6 104 % ±2.8 % 19. 9 19. 9 19. 9 20. 8

21. 4 21. 1 10 5

10 7 10 6 106 % ±0.94 % La b I D LCS-110726-1 0 LCS-110726-1 1 LCS-110726-1 2 Averag e ±%RS D LCS-110726-1 3 LCS-110726-1 4 LCS-110726-1 5 Averag e ±%RS D LCS-110726-1 6 LC8-110726-1 7 LC8-110726-1 8 Averag e ±%RS D PFO S (linear+branched )

Spike d Concenbatio n (ng/mL ) 0.19 8 0.19 8 0.19 8 Calculate d

Concentratio n (ng/mL ) 0.27 2 0.26 4 0.27 1 VoRecover

y 137 13 3 137 136%±1.7%< " 1.98 1.98 1.98 2.2 6

2.3 4 2.4 7 11 4

11 8 12 5 119 % ±4.7 % 19. 8 19. 8 19. 8 22. 9

24 1 25. 6 11 6

12 2 12 9 122 % ±5.3% TM ETS^044. 0 /sjialyze d 7/29/1 1 Externa l standar d calibration La b I D LCS-110726- 4 LCS-110726- 5 LC8-110726- 6 Averag e ±%RS D LCS-110726- 7 LCS-110726- 8 LCS-110726- 9 Averag e ±%RS D PFO S (linear)

Spike d Concentratio n (ng/mL ) 1.99 1.99 1.99 Calculate d

Concentratio n (ng/mL ) 2.0 7 2.0 8 2.0 4 VoRecover

y 104 104 103 104 % ±0.56 % 19. 9 19. 9 19. 9 21. 4

21. 7 20. 1 10 7

10 9 10 1 106 % ±3.9 % La b I D LC8-110726-1 3 LC8-110726-1 4 LCS-110726-1 5 Averag e ±%RS D LC8-110726-1 6 LC8-110726-1 7 LCS-110726-1 8 Averag e ±%RS D PFO S (linear+branched )

Spike d Concentratio n (ng/mL ) 1.98 1.98 1.98 Calculate d

Concentratio n (ng/mL ) 2.0 4 2.0 4 2.2 7 VoRecover

y 103 10 3 11 5 107 % ±6.5 % 19. 8 19. 8 19. 8 17. 2

19. 2 17. 2 87. 1

97. 1 86. 6 90.3 % ±6.6 % (1 ) Th e averag e recover ydi d no tmee t method acceptance criteri a of100 % ±20% .

Pag e 1 9 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



## Tabl e 7 continued . Laborator yContro l Spik e Recovery . ETS^044. 0 Analyze d 8/2/1 1 Intemal standar

d calibration La b I D LCS-110726- 1 LCS-110726- 2 LCS-110726- 3 Averag e ±%RS D LCS-110726 ^ LC8-110726- 5 LC8-110726- 6 Averag e ±%RS D LCS-110726- 7 LCS-110726- 8 LCS-110726- 9 Averag e ±%RS D PFO S (linear)

Spike d Concentratio n (ng/mL ) 0.19 9 0.19 9 0.19 9 Calculate d

Concentratio n Jng/mL ) 0.21 9 0.21 1 0.22 6 VoRecover

y 11 0 10 6 11 4 110 % ±3.6 % 1.99 1.99 1.99 2.1 5

2.0 4 2.1 3 10 8

103 10 7 106 % ±2.5 % 19. 9 19. 9 19. 9 21. 1

21. 7 21. 4 10 6

10 9 10 7 107 % ±1.4 % La b I D LCS-110726-1 0 LCS-110726-1 1 LC8-110726-1 2 Averag e ±%RS D LC8-110726-1 3 LC8-110726-1 4 LC8-110726-1 5 Averag e ±%RS D LCS-110726-1 6 LCS-110726-1 7 LCS-110726-1 8 Averag e ±%RS D PFO S (linear+branched )

Spike d Concentratio n (ng/mL ) 0.19 8 0.19 8 0.19 8 Calculate d

Concentratio n (ng/mL ) 0.25 2 0.25 4 0.26 4 VoRecover

y 12 7 12 8 13 3 129 % ±2.5%*^ ' 1.98 1.98 1.98 2.2 9

2.3 9 2.5 3 l i 6

12 1 12 8 122 % ±5.0% TM 19. 8 19. 8 19. 8 23. 0

24. 3 25. 3 11 6

12 3 12 8 122 % ±4.9% TM (1 ) Th e averag e recover ydi d no t meet metho d acceptance criteri a of100 % ±20% . 

# 8. 7 Analytica l Metho d Uncertaint y Analytica l uncertaint

yi s base d o n historica l Q C dat a thati s contro l charte d an d used t o evaluat e metho d accurac

yan d precision . Th e metho d uncertaint yi s calculate d followin g ETS-12-012.2
. Th e standar d deviatio n i s calculate d fo rth e setofaccurac yresults (i n %) obtaine d fo rth e Q C samples . Th e expande d uncertaint

yi s calculate d b

ymultiplyin g th e standar

d deviatio n b

ya facto rof2 , whic h correspond s t o a confidence leve l o f95% . Th e mos

trecen t5 0 dat a points were use d t o generate th e metho d uncertaint

yvalue s liste d i n Tabl e 8 . Th e metho d uncertaint yfo rPFO S b

yETS-12-012- 2 wa s foun d t o b e ±28% , however , since th e lo wsetoflinea ran d branche d LC S had a n averag e recover yof 136% , th e analytica l uncertaint yassigne d t o thi s stud yfo rPFO S wil l b e ±36% . Tabl e 8 . Analytica l Uncertainty . Analyt e PFB S PFH S PFO S Standar d Deviatio n

5.5 9 7.1 2 NA Metho d Uncertaint y

±11 % ±14 % ±36 % 

## NA= No t Applicabl e

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1



# 8. 8 Fiel d Matri xSpike s (FMS ) Low , mid , an d hig h field matri xspike s (FMS ) were collecte d a teac h samplin g poin t(lowan d hig h FM S onl ya t 131R , 134R , an d 135R) t o verif ythatth e analytica l metho d i s applicabl e t o th e collecte d matrix . Fiel d matri xspike s were generate d b yaddin g a measure d volum e offield sampl e t o a containe rspike d b yth e laborator

ywit h PFB S (linear) , PFH S (linear) , an d PFO S (linear ) prio rt o shippin g sampl e container s fo rsampl e collection . Fiel d matri xspike recoverie s withi n metho d acceptance criteri a of 100±30 % confir m that "unknown " components i n th e sampl e matri xd o no tsignificantl yinterfere wit h th e extractio n an d analysi s ofth e analyte s ofinterest . Fiel d matri xspike concentration s mustb e 50 % ofth e sampl e concentratio n t o b e considere d a n appropriate field spike . Fiel d matri xspike s are presente d i n sectio n 9 ofthi s report . Tabl e 9. Fiel d IVIatri xSpik e Levels . Samplin g Locatio n 1348,1358,136R , 137R , 1378,137 L138 L 130 U 131 S 131 L133R , 135 U 136 S 136L , 141R , 142R , 143R , 144 8 130R , 1308,1338,133 L134L , 138R , 138S , CW26C , CW26L , 140 R 131R , 134R , 135 R Tri p Blan k Spik e Leve l Lo w Mi d Hig h Lo w Mi d Hig h Lo w Mi d Hig h Lo w Hig h Lo w Mi d Mid-Hig h Hig h PFBS , ng/m L

0.10 2 1.02 10. 2 1.0 2 10. 2 102 10. 2 10 2 51 0 102 197 2 1.0 2 10. 2 10 2 51 0 PFHS , ng/m L

0.10 2 1.02 10. 2 1.0 2 10. 2 102 10. 2 102 51 0 102 200 0 1.0 2 10. 2 102 51 0 PFOS , ng/m L

0.098 5 0.98 5 9.8 5 0.98 5 9.8 5 98. 5 9.8 5 98. 5 49 3 98. 5 200 0 0.98 5 9.8 5 98. 5 49 3 (SampleConcentrationo f

FMS - AverageConcentration : FieldSampl e& FieldSampleDup. ) .„,„„„ , FMS Recover

y = -^ ^ ^-^ * 100% Spik eConeentrate

n


## Th e table s belowsummariz e th e sampl e results an d field matri xspike recoverie s fo rth e samplin g location s as wel l as th e Tri p Blank s an d rinseate blanks . Results an d averag e value s are rounde d t o thre e significan t figures accordin g t o EP Aroundin g njles . Becaus e ofrounding , value s ma yvar yslightl y fro m thos e liste d i n th e ra wdata . Fiel d matri xspik

e recoverie s meetin g th e metho d acceptance criteri a o f±30% , demonstrate that th e metho d wa s appropriat e fo rth e give n matri xan d thei rrespective quantitative ranges . DA LG W1 SO

L- Th e recover

yofth e hig h FM S sampl e fo rPFO S wa s 68.3% . Th e hig h spike , th e onl y appropriat e spik

e leve l as compare d t o th e sampl e concentration , i s stil l withi n th e analytica l uncertaint y of100 % ±36% . N o additiona l adjustmen twil l b e mad e t o th e analytica l uncertainfy
. DA LG W131 L- Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 61.2% . Since th e hig h spike wa s th e onl yappropriat e spike leve l as compare d t o th e sampl e concentration , th e analytica l uncertaint yhas bee n adjuste d fo rPFO S t o 100 % ±39% .

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## DA LG W133 L- Th e recover yofth e mi d FM S sampl e fo rPFO S wa s 23 1 % . Th e hig h FM S sampl e met acceptance criteri a wit h a recover yof92.1% . Since bot h spikin g level s were appropriat e as compare d t o th e sampl e concentration , th e sampl e setwil l b e reporte d an d footnoted
. DA LG W134 L- Th e sampl e RP D wa s 22 % fo rPFOS
. DA LG W135 S - Th e recover yo fth e hig h FM S sampl e fo rPFO S wa s 224% . Since th e hig h spike wa s th e onl yappropriat e spike leve l as compare d t o th e sampl e concentration , th e sampl e setwil l no t b e reporte d since th e FM S recover ywa s greate rtha n 100 % ±50% . DA LG W135 L- Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 63.4% . Since th e hig h spik e wa s th e onl yappropriat e spike leve l as compare d t o th e sampl e concentrafion , th e analytica l uncertaint yhas bee n adjuste d fo rPFO S t o 100 % ±37% . DA LG W136 L- Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 58.9% . Since th e hig h spike wa s th e onl yappropriat e spike leve l as compare d t o th e sampl e concentration , th e analytica l uncertaint yhas bee n adjuste d fo rPFO S t o 100 % ±4 1 % . DA LG W141 R - Th e recover yo fth e hig h FM S sampl e fo rPFO S was 65.6% . Th e mi d FM S sampl e met metho d acceptance criteria . Since th e mi d spik e wa s th e mos

tappropriat e spik

e leve l as compare d t o th e sampl e concentration , th e analytica l uncertaint ywil l no tb e iadjusted fo rPFOS
. DA LG W142 R - Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 68.5% . Th e hig h spike , th e onl y appropriat e spik

e leve l as compare d t o th e sampl e concentration , i s stil l withi n th e analytica l uncertaint y of100 % ±36% . N o additiona l adjustmen twil l b e mad e t o th e analytica l uncertainty . DA LG W144 S - Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 63.5% . Since th e hig h spik e was th e onl yappropriat e spike leve l as compare d t o th e sampl e concentration , th e analytica l uncertaint yhas bee n adjuste d fo rPFO S t o 100 % ±37% .

Pag e 2 2 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 10 . DA LG W130R11063 0

3MUMSI D GLP10-O1-O2-1&<30

6 GLP10-01-02-18-00 7 GLP10-01-02-18-00 8 GLP10-01-02-18^0 9 GLPIO^I-02-18-01 0 Averag e Concent i Descriptio n

DA LGW130 R 011063 0 DA LGW130RD B 110630 DA LGW130 R LS11063 0 DALGW130RMS11063 0 DALGW130RH811063 0 ratio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 16. 4 16. 1 25. 0 11 0 50 1 VoRecover

y N A N A 85. 8 91. 9 95. 0 16. 3 ng/m L±1.8 % PFH S Concentratio n (ng/mL ) 12 5 12 5 13 1 21 8 62 9 VoRecover

y N A N A N C 91. 2 98. 8 12 5 ng/m L±0.0 % PFO S Concentratio n (ng/mL ) 44 0 46 0 50 1 49 2 85 3 VoRecover

y N A N A N C N C 81. 8 45 0 ng/m L±4.4 % NA= No t Applicabl e N C = No t Calculated ; th e endogenou s sampl e concentratio n i s greate rtha n 2 xth e spike level . Reporte d results fro m externa l standar d calibration . 

## Tabl e 11 . DA LG W1 SO S 1106S 0

3MUMSi D GLP10-01-02-18-01 1 GLP10-01-02-18-01 2 GLP10-01-02-18-01 3 GLP10-01-02-18-01 4 GLP10-01-02-18-01 5 Averag e Concent i Descriptio n

DALG W130 8 011063 0 DALGW130SDB11063 0 DALGW130SL811063 0 DALGW130 S M S 110630 DALGW130SH S 110630 •atio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 3.5 3 3.5 3 13. 7 10 2 50 4 VoRecover

y NA NA 100 96. 5 98. 4 3.5 3 ng/m L±0.0 % PFH S Concentratio n (ng/mL ) 29. 8 29. 7 39. 9 130 57 7 %Recover

y NA NA N C 98 3 107 29. 8 ng/m L±0.34 % PFO S Concentratio n (ng/mL ) 100 95. 1 11 3 185 46 6 %Recover

y N A N A N C 88. 8 74. 8 97. 6 ng/m L±5.0 % NA= Not Applicabl e N C = No t Calculated ; th e endogenou s sampl e concentratio n I s greate rtha n 2xth e spik e level . Reporte d results from externa l standar d calibration .

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### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 12 . DA LG W130 L11063 0

3 M LIM S I D GLP10-01-02-18-01 6 GLP10-01-02-18-01 7 GLP10^1-02-18-01 8 GLP1O-01-O2-18-01 9 GLP10-01-02-18-02 0 Descriptio n DA LGW130 L011063 0 DA LGW130LD B 110630 DA LGW130LL S 110630 DA LGW130 LM S 110630 DALGW130 LH S 110630 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 1.6 8 1.6 5 2.7 7 12. 4 98. 2 VoRecover

y NA NA 10 8 10 5 94. 6 1.67 ng/m L±1.8 % PFH S Concentratio n 

## Jng/mL) 13. 7 13. 4 15. 2 24 1 11 4 VoRecover

y N A NA N C 103 98. 5 13. 6 ng/m L±2.2 % PFO S Concentratio n (ng/mL ) 62. 0 65. 4 72. 0 72. 4 13 1 VoRecover

y N A N A N C N C 68 3 <^ ' 63. 7 ng/m L±5.3 % N A= Not Applicabl e N C = Not Calculated ; th e endogenou s sampl e concentratio n i s greate rtha n 2xth e spik e level . Reporte d iBsult

s from extema l standar

d calibration . (1 ) FM S di d no

t inee t acceptance criteri a of100±30% . Th e recover yI s withi n th e analytica l uncertainty . 

## Tabl e 13 . DALG W131R11062 2

3 M LIM S I D GLP10-01-02-18-02 1 GLP10-01-O2-18-022 GLP10^1-02-18-02 3 GLP10-01-02-18^2 4 Descriptio n DA LGW131 R 011062 2 DA LGW131RD B 110622 DA LGW131RL 8 110622 DA LGW131RH S 110622 Averag e Concentrati'o n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 53. 9 55. 8 157 188 0 VoRecover

y NA NA 100 92. 6 54. 9 ng/m L±3.5 % PFH S Concentratio n (ng/mL ) 29 7 31 1 41 4 220 0 VoRecover

y N A N A N C 94. 8 304 ng/m L±4.6 % PFO S Concentratio n (ng/mL ) 1530 1480 NA<^ ' 320 0 VoRecover

y N A N A NA<^ ' 84. 8 151 0 ng/m L±3.3 % N A= Not Applicabl e N C = Not Calculated ; th e endogenou s sampl e concenfratio n i s greate rtha n 2xth e spik e level . Reporte d iBsults from extema l standar d calibration . (1 ) FM S no

t analyze d since spike leve l was no t appropriate i n compariso n t o th e sampl e concentration .

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### GLP10-01-02 ; Interi m Repor t 1 8 /^alysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 14 . DA LG W131 S 11062 2

3 M LIM S I D GLP10-01-O2-18-025 GLP10-01-02-18-02 6 GLP10-01-02-18-02 7 GLP10^1-02-18-02 8 GLP10-01-02-1&O2 9 Averag e Concent i Descriptio n

DALG W131 8 011062 2 DALGW131SD B 110622 DALGW131SL S 110622 DALGW131 8 M S 110622 DALGW1318H S 110622 ^ o n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 0.91 4 0.873 1.9 3 10 9 98. 9 VoRecover

y NA NA 10 2 98 1 96. 1 0.89 4 ng/m L±4.6 % PFH S Concentratio n (ng/mL ) 5.2 6 4.8 8 6.0 7 15. 2 10 4 VoRecover

y N A N A N C 99. 3 97. 0 5.0 7 ng/m L±7.5 % PFO S Concentratio n (ng/mL ) 5.3 1 5.42 6.2 7 13. 1 80. 3 VoRecover

y N A N A N C 78. 5 76. 1 5.3 7 ng/m L±2.1 % N A= No t /Vpplicabl e N C = No t Calculated ; th e endogenou s sampl e concentratio n i s greate rtha n 2xth e spik e level . Reported results from extema l standar d calibration . 

## Tabl e 15 . DALGW131 L11062 2

3MUMSI D GLP10-01-02-18-03 0 GLP10^1-02-18-03 1 GLP10-01-02-18-032 GLP10^1-02-18-03 3 GLP10-01-02-18-034 Averag e Concent i Descriptio n

DA LGW131 L011062 2 DA LGW131LD B 110622 DA LGW131LL S 110622 DA LGW131 LM S 110622 DA LGW131LH S 110622 ratio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 34 0 3.44 44 4 13 8 102 VoRecover

y NA NA N C 102 96. 6 3.42 ng/m L±1.2 % PFH S Concentratio n (ng/mL ) 21. 4 21. 8 22. 8 32. 8 12 0 VoRecover

y NA NA N C N C 96. 5 21. 6 ng/m L±1.9 % PFO S Concentratio n (ng/mL ) 32. 6 32. 1 30. 5 42. 0 92. 6 %Recover

y N A N A N C N C 61.2 TM 32. 4 ng/m L±1.5 % ^^

> N A= No t /\pplicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2 xspike level . Reporte d results liti m extema l standar d calibration . (1 ) FM S di d notmeetacceptance criteri a of100±30% . Th e analytica l uncertaint yfo rPFO S has bee n adjuste d fo rFM S recover yt o ±39% .

Pag e 2 5 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 16 . DA LGW133R11603 0

3MUMSi D GLP10-01-02-18-045 GLP10-01-02-18^>46 GLP10-01-O2-18O47 GLP10-01-02-18-04 8 GLP10-01-02-18-04 9 Averag e Concent i Descriptio n

DA LGW133 R 011063 0 DALGW133RDB11063 0 DA LGW133 R LS11063 0 DALGW133RM811063 0 DALGW133RHS11063 0 atio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 4.7 0 48 8 5.62 14 3 98 9 %Recover

y NA NA N C 93. 2 92. 3 4.7 9 ng/m L±3.8 % PFH S Conceittratio n (ng/mL ) 32. 6 33. 6 32. 0 41. 7 130 VoRecover

y NA NA N C N C 95. 0 33. 1 ng/m L±3.0 % PFO S Concentratio n (ng/mL ) 44. 1 41. 0 37. 5 51. 3 11 1 %Recover

y NA NA N C N C 69.5 TM 42. 6 ng/m L±7.3 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Repotte d results from extema l standar d calibration . (1 ) FM S di d no

tmee t acceptance criteri a of100±30%. . Th e recover yi s withi n th e analytica l uncertaint y 

## Tabl e 17 . DA LG W13S S 11063 0

3 M LIM S I D GLP10-01-O2-1&O40 GLP10^1-02-18-04 1 GLPIO^I-02-18^ 2 GLP10-01-02-18-043 GLP10-01-02-18-044 Averag e Concent i Descriptio n

DALG W133 8 011063 0 DALGW1338D B 110630 DA LG W133 8 LS 110630 DALG W133 8 M S 110630 DALGW1338H S 110630 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 9.0 1 9.1 4 18. 9 105 48 7 VoRecover

y N A N A 96. 3 94. 0 93. 7 9.0 8 ng/m L±1.4 % PFH S Concentratio n (ng/mL ) 66. 9 67. 4 76. 2 16 7 55 1 VoRecover

y N A NA N C 97. 9 94. 9 67. 2 ng/m L±0.74 % PFO S Concentratio n (ng/mL ) 19 6 194 20 2 26 5 58 5 VoRecover

y N A NA N C 71. 1 79. 2 195ng/mL±1.0V o N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d results from extema l standar d calibration .

Pag e 2 6 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 18 . DA LGW 1 SS L11063 0

3 M LIM S I D GLP10-01-02-18-035 GLP10-01-02-18-03 6 GLP10-01-02-18-03 7 GLP10-01-02-18-03 8 GLP10-01-02-18-03 9 Averag e Concen t Descriptio n

DA LGW133 L011063 0 DA LGW133LD B 110630 DA LGW133 LLS11063 0 DALGW133LMS11063 0 DALGW133LH811063 0 ratio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 6.6 8 6.6 5 17. 2 107 48 7 VoRecover

y NA NA 103 98. 4 94. 2 6.6 7 ng/m L±0.45 % PFH S Concentratio n (ng/mL ) 47. 3 46. 5 61. 6 164 54 3 VoRecover

y NA NA N C 11 5 97. 3 46. 9 ng/m L±1.7 % PFO S Concentratio n (ng/mL ) 149 14 2 NA « 37 3 59 9 VoRecover

y NA N A NA TM 23 1 <" ' 92. 1 146 ng/m L±4.8 % N A= No t Applicabl e N C = Not Calculated ; Endogenou s sampl e concentratio n greate rtha n 2 xspike level . Reporte d results Iro m extema l standar d calibration . (1 ) Not Analyzed ; Th e L S was no tanalyze d since i t was no t expecte d t o b e appropriate as compare d t o th e sampl e concentration . (2 ) Fiel d mabi xspik

e di d notmee

t method acceptance criteri a of100±30% . N o adjusfrnen t was mad e t o th e analytica l uncertaint ysince bot h spik

e level s wer

e appropriate a

s compare d t o th e sampl e concentration . 

## Tabl e 19 . DA LGW134R11062 8

3MUMSI D GLP10-01-02-18-05 0 GLP10-01-02-18-05 1 GLP10-01-02-18-052 GLP10-01-02-18-05 3 Descriptio n DA LGW134 R 011062 8 DALGW134RDB11062 8 DA LGW134RL 8 11062 8 DA LGW134RH 8 11062 8 Averag e Concerrtratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 51. 7 51. 0 150 189 0 VoRecover

y NA NA 96. 7 93 2 51. 4 ng/m L±1.4 % PFH S Concentratio n (ng/mL ) 48 4 48 1 58 8 236 0 VoRecover

y NA NA N C 93. 9 48 3 ng/m L±0.62 % PFO S Concentratio n (ng/mL ) 99 6 1130 1040 287 0 VoRecover

y NA NA N C 90. 4 106 0 ng/m L±13 % N A= Not Applicabl e N C = Not Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2 xspike level . Reported results from extema l standar d calibration .

Pag e 2 7 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 20 . DALG W1S4S 11062 8

3 M LIM S I D GLP10-01-02-18-05 9 GLP10-01-02-18-06 0 GLP10-01-02-18-06 1 GLP10-01-02-18-062 GLP10-01-02-18-06 3 Averag e Concen t Descriptio n

DALG W134 8 011062 8 DALGW134SDB11062 8 DALG W134 8 LS11062 8 DALGW134SMS11062 8 DALGW1348HS11062 8 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 0.25 5 0.26 1 0.37 1 1.2 3 10. 8 VoRecover

y NA NA N C 95. 3 103 0.25 8 ng/m L±2.3 % PFH S Concentratio n (ng/mL ) 3.05 31 2 31 2 4.0 8 14 0 VoRecover

y N A N A N C N C 107 3.0 9 ng/m L±2.3 % PFO S Concentratio n (ng/mL ) 13. 3 13. 3 12. 6 14. 6 24. 9 VoRecover

y NA NA N C N C 11 8 13. 3 ng/m L±0.0 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspike level . Reporte d results \co m extema l standar d calibration . 

## Tabl e 21 . DA LG W1S4 L11062 8

3MUMSI D GLP1CW)1-02-18-05

4 GLP10-01-O2-18-055 GLP1001-02-18-05 6 GLP10-01-02-18-05 7 GLP1OO1-02-18-05 8 Averag e Concent i Descriptio n

DA LGW134 L011062 8 DA LGW134LD B 11062 8 DA LGW134LL S 11062 8 DA LGW134 LM S 11062 8 DA LGW134LH S 11062 8 'atio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 11. 4 12. 2 22. 0 10 8 47 9 VoRecover

y NA NA 100 94. 3 91. 6 11.8ng/mL±6.8V o PFH S Concentratio n (ng/mL ) 10 4 11 8 127 21 6 58 6 VoRecover

y N A N A N C 103 93. 1 11 1 ng/m L±13 % PFO S Concentratio n (ng/mL ) 43 8 547 46 9 534 89 7 VoRecover

y NA NA N C N C 82. 1 49 3 ng/m L±22 % <^

> NA = Not/Vpplicabl e N C = Not Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2 xspike level . Reporte d results from extema l standar d calibration . (1 ) RP D di d no

tmeet acceptance criteri a ofs20% .

Pag e 2 8 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Table22 . DALG W135 R 11062 8

3 M LIM S I D GLP10-01-02-18-07 9 GLP10-01-02-18-08 0 GLP10-01-O2-18-08 1 GLP10-01-02-18-08 3 Descriptio n DA LGW135 R 011062 8 DALGW135RDB11062 8 DA LGW135 R LS11062 8 DA LGW135RH 8 11062 8 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 74. 9 73. 7 17 2 1840 VoRecover

y NA NA 95. 8 89. 5 74. 3 ng/m L±1.6 % PFH S Concentratio n (ng/mL ) 85 9 82 8 92 7 262 0 VoRecover

y NA NA N C 88. 8 84 4 ng/m L±3.r/ o PFO S Concentratio n (ng/mL ) 102 0 91 7 1070 248 0 VoRecover

y N A NA N C 75. 6 96 9 ng/m L±11 % N A= Not Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level . Reported results fro m extema l standar d calibration . 

## Tabl e 23 . DALG W135 S 11062 8

3 M LIM S I D GLP10-01-02-18-06 9 GLP10-01-02-18-07 0 GLP1001-02-18-07 1 GLP10-01-02-18-072 GLP10-01-02-18-073 Averag e Concen t Descriptio n

DALG W135 8 011062 8 DALG W135 8 D B 11062 8 DALG W135 8 LS 11062 8 DALG W135 8 M S 11062 8 DALG W135 8 H S 11062 8 ratio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 1.20 1.2 1 1.4 1 2.2 1 10. 5 VoRecover

y NA NA N C 98. 5 91. 1 1.2 1 ng/m L±0.83 % PFH S Concentratio n (ng/mL ) 7.9 7 8.1 5 88 6 9.2 2 19. 1 VoRecover

y NA NA N C N C 108 8.0 6 ng/m L±2.2 % PFO S Conceittratio n (ng/mL ) VoRecover y

No t Reporte d '^'

N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level . Reporte d results fo rPFB S an d PFH S are from externa l standar d calibration . PFO S was analyze d b ybot h intema l standar d an d extema l standar

d calibratio n wit h th e results from bot h analyses bein g no

t reportable . (1 ) No t Reported ; ttie mos

tappropriate field mafri xspik e recover ywas greate rtha n 100 % ±50% .

Pag e 2 9 of11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 24 . DA LG W135 L11062 8

3 M LIM S I D GLP1OO1-02-18-07

4 GLP10-01-02-18-075 GLP10-01-02-18-07 6 GLP10-01-02-18-077 GLP10-01-02-18-07 8 Averag e Concen t Descriptio n

DA LGW135 L011062 8 DA LGW135LD B 11062 8 DA LGW135LL 8 11062 8 DA LGW135 LM S 11062 8 DA LGW135LH 8 11062 8 'atio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 9.73 9.65 10. 6 19. 9 109 VoRecover

y NA NA N C 100 97. 4 9.6 9 ng/m L±0.83 % PFH S Concentratio n (ng/mL ) 71. 9 70. 1 73. 9 84. 0 176 VoRecover

y N A N A N C N C 103 71. 0 ng/m L±2.5 % PFO S Concentratio n (ng/mL ) 55. 5 59. 7 48. 1 57. 8 12 0 VoRecover

y NA NA N C N C 63.4 TM 57. 6 ng/m L±7.3%'^ ' NA = Not/Vpplicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reported results from extema l standar d calibration . (1 ) FM S di d no

t mee tacceptance criteri a of100±30% . Th e analytica l uncertaint yfo rPFO S has bee n adjuste d torFM S recover yt o ±37% . 

## Tabl e 25 . DA LG W136 S 11063 0

3 M LIM S I D GLP10-01-02-18-08 8 GLP10-01-02-18-08 9 GLP10-01-02-18-09 0 GLP10-01-02-18-09 1 GLP10-01-02-18-092 Averag e Concen t Descriptio n

DALG W136 8 011063 0 DALGW136SDB11063 0 DALGW1368LS11063 0 DALG W136 8 M S 110630 DALGW136SH811063 0 'atio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 37 1 36 7 46 1 13. 2 99. 8 VoRecover

y NA NA N C 93 2 94. 2 3.6 9 ng/m L±1.1 % PFH S Concentratio n (ng/mL ) 25. 9 25. 7 26. 7 35. 2 125 VoRecover

y N A N A N C N C 97. 3 25. 8 ng/m L±0.78 % PFO S Concentratio n (ng/mL ) 11 5 11 6 12 0 11 8 187 %Recover

y NA N A N C N C 72. 6 11 6 ng/m L±0.87 % NA = No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level . Reported results from extema l standar d calibration .

Pag e 3 0 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 26 . DA LG W136 L11063 0

3MUMSI D GLP10-01-02-18-084 GLP10-01-02-18-08 5 GLP10-01-02-18-08 6 GLP10-01-02-18-15 5 GLP1OO1-02-18-087 Averag e Concen t Descriptio n

DA LGW136 L011063 0 DA LGW136LD B 11063 0 DA LGW136 LLS11063 0 DALGW136 LM S 11063 0 DALGW136LH S 11063 0 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 2.3 3 2.32 3.32 12. 5 10 1 %Recover

y NA NA N C 99. 8 96. 7 2.3 3 ng/m L±0.43 % PFH S Concentratio n (ng/mL ) 15. 9 16. 1 17. 0 26. 3 11 5 VoRecover

y NA NA N C 10 1 97. 1 16. 0 ng/m L±1.3 % PFO S Concentratio n (ng/mL ) 72. 4 65. 5 73. 6 84. 8 12 7 VoRecover

y NA NA N C N C 58.9 TM 69.0ng/mL±10%'^ > N A= Not Applicabl e N C = Not Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2 xspik e level . Reporte d results from extema l standar d calibration . (1 ) FM S di d notmeet acceptanc e criteri a of1 oa±30% . Th e analytica l uncertaint yfo rPFO S has bee n adjuste d ft)rFM S recover yto ±4 1 % . 

## Tabl e 27 . DA LG W1S7S 11062 4

3MUMSI D GLP10-01-02-18-O93 GLP10-01-02-18-094 GLP10-01-02-1&O9 5 GLP10-01-02-18-09 6 GLP10-01-02-18-09 7 Averag e Concen t Descriptio n

DALG W137 8 011062 4 DALGW1378D B 11062 4 DA LGW137SL 8 11062 4 DALG W137 8 M S 110624 DALGW137SH811062 4 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 0.10 8 0.105 0.21 5 1.0 5 9.6 8 VoRecover

y NA N A 106 92. 5 93. 9 0.10 7 ng/m L±2.8 % PFH S Concentratio n (ng/mL ) 0.081 4 0.079 8 0.184 1.0 6 9.8 6 VoRecover

y NA NA 10 1 96. 0 95. 9 0.080 6 ng/m L±2.0 % PFO S Concentratio n (ng/mL ) 0.49 8 0.467 0.56 9 1.4 2 10. 2 VoRecover

y N A NA N C 95. 2 98. 7 0.48 3 ng/m L±6.4 % NA = No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d results fo rPFB S an d PFH S ar e fro m extema l standar

d calibration . Reporte d results fo rPFO S ar e from intema l standar

d calibration .

Pag e 3 1 of11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 28 . DA LGW 1 ST L11062 4

3 M LIM S I D GLP10-01-02-18-09 8 GLP10-01-02-18-09 9 GLP10O1-02-18-10 0 GLP10-01-O2-18-10 1 GLP10-01-02-18-102 Averag e Concent i Descriptio n

DA LGW137 L011062 4 DALGW137LDB11062 4 DA LGW137 LLS11062 4 DALGW137LM811062 4 DALGW137LH811062 4 -atio n (ng/mL ) ±VoRPD PFB S Concentiatio n (ng/mL ) 0.0834 0.081 8 0.18 9 1.0 6 9.7 0 VoRecover

y NA NA 10 4 95. 8 94. 3 0.082 6 ng/m L±1.9 % PFH S Concentratio n (ng/mL ) 0.10 6 0.107 0.21 3 1.1 0 10. 0 VoRecover

y NA NA 104 97. 4 97. 0 0.10 7 ng/mL ±0.94 % PFO S Concentiatio n (ng/mL ) 1.9 3 1.9 8 2.0 1 2.9 6 12. 0 %Recover

y N A N A N C 10 2 102 1.96 ng/m L±2.6 % NA = No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspike level . Reporte d results fo rPFBS an d PFH S ar e from extema l standar

d calibiration . Reporte d results fo rPFO S are from intema l standar d calibration . 

## Tabl e 29 . DA LG W138 S 11063 0

3MUMSI D GLP10-01-02-18-11 3 GLP10-01-02-18-11 4 GLP10-01-02-18-11 5 GLP10-01-02-18-11 6 GLP10-01-02-18-11 7 Averag e Concent i Descriptio n

DA LGW138 S 011063 0 DALGW138SDB11063 0 DALGW138SLS11063 0 DALGW138SM811063 0 DALGW138SH811063 0 -atio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 4.5 5 4.5 8 14 5 96. 8 48 8 %Recover

y NA N A 97. 4 90. 4 94. 8 4.5 7 ng/m L±0.66 % PFH S Concentratio n (ng/mL ) 23. 2 23. 2 33. 4 11 7 52 8 VoRecover

y NA NA N C 92. 0 99. 0 23. 2 ng/m L±0.0 % PFO S Concentiatio n (ng/mL ) 194 20 3 22 1 26 9 59 3 VoRecover

y NA NA N C N C 80. 1 19 9 ng/m L±4.5 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspike level . Reporte d results from extema l standar d calibration .

Pag e 3 2 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1



## Tabl e 30 . DALGW1S8 L1106S 0

3 M LIM S I D GLP1001-02-18-10 8 GLP10-01-02-18-10 9 GLP10-01-02-18-11 0 GLP10-01-02-18-11 1 GLPiaOI-02-18-11 2 Averag e Concen t Descriptio n

DA LGW138 L011063 0 DA LGW138LD B 110630 DA LGW138LL 8 110630 DA LGW138 LM S 110630 DALGW138LHS11063 0 -atio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 0.0385 0.039 6 0.135 0.973 9.0 6 VoRecover

y N A N A 94. 1 91. 6 88. 4 0.039 1 ng/m L±2.8 % PFH S Concentratio n (ng/mL ) 0.11 2 0.11 3 0.21 3 1.0 8 9.82 %Recover

y NA N A 98. 5 94. 9 95. 2 0.11 3 ng/mL ±0.89 % PFO S Concentratio n (ng/mL ) 0.840 0.862 1.0 4 1.8 2 10. 8 VoRecover

y NA NA N C 98. 4 10 1 0.85 1 ng/m L±2.6 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d result

s fo rPFB S an d PFH S are from extema l standar d calibration . Reporte d results fo rPFO S are from Intema l standar d calibration . 

## Tabl e S1
. DA LG W1S8R11063 0

3MUMSI D GLP1OO1-02-18-103 GLP10-01-02-18-104 GLP10-01-02-18-10 5 GLP10-01-02-18-10 6 GLPiaO1-02-18-10 7 Averag e Concen t Descriptio n

DA LGW138 R 011063 0 DA LGW138RD B 110630 DALGW138RLS11063 0 DA LGW138 R M S 110630 DALGW138RHS11063 0 ratio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 8.0 1 8.0 1 17. 7 97. 8 46 2 VoRecover

y N A N A 95. 0 88. 0 89. 0 8.0 1 ng/m L±0.0 % PFH S Concentratio n (ng/mL ) 39. 1 39. 2 48. 8 134 51 5 VoRecover

y N A N A N C 93. 0 93. 3 39. 2 ng/mL ±0.26 % PFO S Concentratio n (ng/mL ) 34 8 34 6 34 0 40 5 73 1 VoRecover

y N A N A N C N C 78. 0 34 7 ng/m L±0.58 % N A= No t /Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d result

s fro m extema l standar

d calibration .

Pag e 3 3 of11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 32 . DA LG WCW26 C 11062 2

3MUMSI D GLP10-01-02-18-11 8 GLP10-01-02-18-11 9 GLP10-01-O2-18-12 0 GLP10-01-02-18-12 1 GLP10-01-02-18-12 2 Descriptio n

DA LGWCW26 C 011062 2 DA LG WCW26 C D B 110622 DA LG WCW26 C LS 110622 DA LG WCW26 C M S 110622 DA LG WCW26 C H S 110622 Averag e Concentratio n (ng/mL ) ±VoRPD PFB S Concentiatio n (ng/mL ) 8.2 6 8.2 0 18. 1 10 6 48 0 VoRecover

y NA NA 96. 8 95. 9 92. 5 8.2 3 ng/m L±0.73 % PFH S Concentratio n (ng/mL ) 68. 3 67. 2 77. 0 166 56 3 VoRecover

y NA NA N C 96. 3 97. 1 67. 8 ng/mL ±1.6

% PFO S

Concentiatio n (ng/mL ) 39 8 39 8 38 4 49 9 83 9 VoRecover

y NA NA N C N C 89. 5 39 8 ng/m L±0.0 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reported results from extema l standar d calibration . 

## Tabl e 33 . DA LG WCW26 L11062 2

3MUMSI D GLP10-01-02-18-12 3 GLP10-O1-02-18-124 GLP10-01-02-18-12 5 GLP10-01-02-18-12 6 GLP10-01-02-18-12 7 Descriptio n

DA LGWCW26 L011062 2 DA LG WCW26 LD B 110622 DA LG WCW26 LLS 110622 DA LG WCW26 LM S 110622 DA LGWCW26LH S 11062 2 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 13. 4 13. 5 23. 0 10 7 50 1 VoRecover

y NA NA 93. 6 91. 7 95. 6 13. 5 ng/m L±0.74 % PFH S Concentratio n (ng/mL ) 104 105 11 5 20 2 59 9 VoRecover

y NA NA NC 95. 6 97. 0 105ng/mL±1.0 % PFO S Concentratio n (ng/mL ) 91 1 95 7 92 5 98 5 141 0 %Recover

y NA NA N C N C 96. 6 93 4 ng/m L±4.9 % NA = No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reported results from extema l standar d calibration .

Pag e 3 4 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 34 . DA LG W140 R 11062 2

3MUMSI D GLP10-01-02-18-12 8 GLP10-01-02-18-12 9 GLP10-01-02-18-13 0 GLP10-01-02-18-13 1 GLP10-01-O2-18-132 Descriptio n

DA LGW140 R 011062 2 DA LGW140RD B 11062 2 DA LGW140 R LS11062 2 DA LGW140 R M S 110622 DA LGW140RH 8 110622 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 1.5 8 1.6 0 11. 3 10 1 47 7 VoRecover

y NA N A 95. 2 97. 5 93. 2 1.59ng/mL±1.3 % PFH S Concentratio n (ng/mL ) 17. 7 17. 9 27. 8 11 9 50 8 VoRecover

y NA N A 98. 0 99. 2 96. 1 17. 8 ng/mL ±1.1 % PFO S Concentratio n (ng/mL ) 11 9 136 125 21 0 59 8 VoRecover

y N A N A N C 83. 8 95. 5 12 8 ng/m L±13 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d result

s from extema l standar

d calibration . 

## Tabl e 35 . DA LG W141 R 11063 0

3 M LIM S I D GLP1001-02-18-13 5 GLP10-01-02-18-13 6 GLP10-01-02-18-13 7 GLP10-01-02-18-13 8 GLP1OO1-02-18-139 Averag e Concen t Descriptio n

DA LGW141R 0 110630 DALG W14 1 RD B 110630 DA LG W141RL S 11063 0 DALG W14 1 RM S 110630 DA LG W141RH S 110630 -atio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 0.0437 0.042 5 0.992 9.4 9 94. 7 VoRecover

y NA NA 93. 0 92. 6 92. 8 0.043 1 ng/m L±2.8 % PFH S Concentratio n (ng/mL ) 0.29 8 0.29 3 1.3 0 10. 0 99. 1 VoRecover

y NA NA 98. 5 95. 1 96. 9 0.29 6 ng/m L±1.7 % PFO S Concentiatio n (ng/mL ) 3.04 2.9 5 3.9 4 10. 0 67. 6 VoRecover

y NA NA N C 71. 1 65.6 TM 3.0 0 ng/m L±3.0 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2xspik e level . Reporte d result

s from extema l standar

d calibration . (1 ) FM S di d not meet acceptance criteri a of100±30% . Th e recover yi s wittii n th e analytica l uncertainty .

Pag e 3 5 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 36 . DA LG W142R11062 2

3MUMSI D GLP10-01-02-18-14 0 GLPiaO1-02-18-14 1 GLP10-01-02-18-14 2 GLP10-01-02-18-14 3 GLP10-01-02-18-14 4 Averag e Concen t Descriptio n

DA LGW142 R 011062 2 DA LGW142RD B 11062 2 DA LGW142RL 8 11062 2 DA LGW142 R M S 11062 2 DALGW142RH S 11062 2 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 1.6 9 1.6 9 2.6 2 11. 4 97. 0 VoRecover y



## NA NA 91. 2 95. 2 93. 4 1.6 9 ng/m L±0.0 % PFH S Concentratio n (ng/mL ) 10. 0 10. 3 11. 1 19. 6 

## 109 VoRecover y



## NA NA N C 92. 6 96. 9 10. 2 ng/m L±3.0 % PFO S Concentratio n (ng/mL ) 82. 6 88. 4 89. 3 85. 2 

## 153 VoRecover y



## N A N A N C N C 685 TM 85. 5 ng/m L±6.8 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspike level . Reporte d result

s from extema l standar

d calibration . (1 ) FM S di d no

t meet acceptance criteri a of100t30% . Th e recover yi s withi n th e analytica l uncertainty . 

## Tabl e 37 . DA LG W143R11062 2

3MUMSI D GLP10-01-02-18-14 5 GLP10-01-02-18-14 6 GLP10-01-02-18-14 7 GLP10-01-02-18-14 8 GLP10-01-02-18-14 9 Averag e Concen t Descriptio n

DA LGW143 R 011062 2 DALGW143RD B 11062 2 DALGW143RL S 11062 2 DA LGW143 R M S 11062 2 DALGW143RH S 11062 2 ratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 1.6 4 1.6 8 2.6 2 10. 9 96. 0 %Recover y



## N A N A 94. 1 90. 6 92. 5 1.6 6 ng/m L±2.4 % PFH S Concentratio n (ng/mL ) 5.9 4 6.1 7 7.0 4 15. 4 

## 10 6 VoRecover y



## N A N A N C 91. 6 98. 0 6.0 6 ng/m L±3.8 % PFO S Concentratio n (ng/mL ) 23. 4 

## 24 2 NA<^ > 32. 3 

## 12 3 VoRecover y



## N A N A NA<^ ' 

## N C 10 1 23. 8 ng/m L±3.4 % NA = Not Applicabl e N C = Not Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level . Reporte d result

s from extema l standar

d calibration . (1 ) FM S no

t analyze d since spik

e leve l was no

tappropriate i n compariso n t o th e sampl e concentration .

Pag e 3 6 of11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 38 . DA LG W144 S 11062 8

3MUMSI D GLP10-01-02-18-15 0 GLP10-01-02-18-15 1 GLP10-01-02-18-152 GLP10-01-02-18-15 3 GLP10-01-O2-18-154 Averag e Concen t Descriptio n

DA LGW144 S 011062 8 DA LGW144SD B 11062 8 DALG W144 8 L S 11062 8 DA LGW144 S M S 11062 8 DA LGW144SH S 11062 8 atio n (ng/mL ) ±VoRPD PFB S Concentratio n (ng/mL ) 1.69 1.6 2 2.5 5 11. 4 91. 4 VoRecover

y N A NA 87. 7 95. 5 88. 0 1.6 6 ng/m L±4.2 % PFH S

Concentiatio n (ng/mL ) 7.2 8 7.0 0 7.9 9 17. 1 98. 5 VoRecover

y N A NA N C 97. 6 89. 6 7.1 4 ng/m L±3.9 % PFO S Concentratio n (ng/mL ) 29. 5 28. 4 NA<^ ' 37. 0 91. 5 VoRecover

y N A N A NA*^ ' N C 63. 5 <^ ' 29. 0 ng/m L±3.8%^

^ N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concenfratio n greate rtha n 2 xspike level . Reporte d result

s fro m extema l standar

d calibration . (1 ) FM S no

t analyze d since spike leve l was not appropriate i n compariso n t o th e sampl e concentration . (2 ) FM S di d not meet acceptance criteri a of100t30% . Th e analytica l uncertaint yfo rPFO S has bee n adjuste d fo rFM S recover yt o ±37% . 

## Tabl e 39 . Tri p Blan k

1

3MUMSI D GLP10-01-02-18-064 GLP10-01-02-18-06 5 GLP10-01-02-18-06 6 GLP10-01-02-18-06 7 GLP10-01-02-18-06 8 Descriptio n Tri p Blan k

Set 1 Sampl e Tri p Blan k

Set 1 LS Tri p Blan k

Se t 1 M S Tri p Blan k

Set 1MH S Tri p Blan k

Set 1H S PFB S

Concentratio n (ng/mL ) <0.025 0 1.0 5 10. 3 102 50 8 VoRecover

y NA 103 10 1 100 99. 6 PFH S

Concentratio n (ng/mL ) <0.025 0 1.1 0 10. 9 10 5 52 3 %Recover

y NA 108 107 103 103 PFO S Concentratio n (ng/mL ) <0.10 0 1.0 1 9.45 10 2 38 1 %Recovei y

NA 103 95. 9 104 77. 4 NA = Not Applicabl e Reporte d results from extema l standar d calibration .

Pag e 3 7 of11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Tabl e 40 . Tri p Blan k

2

3MUMSI D GLP10-01-02-18-00 1 GLP10-01-02-18-O02 GLP10-01-O2-18-O03 GLP10-O1-O2-18-00

4 GLP10-01-02-18-00 5 Descriptio n Tri p Blan k

Set 2 Sampl e Tri p Blan k

Set 2 LS Tri p Blan k

Set 2 M S Tri p Blan k

Set 2 MH S Tri p Blan k

Set 2 H S PFB S

Concentratio n (ng/mL ) <0.025 0 0.99 5 9.62 93. 0 45 9 VoRecover

y NA 97. 5 94. 3 91. 2 90. 0 PFH S

Concentratio n (ng/mL ) <0.025 0 1.0 1 9.9 8 95. 2 47 7 VoRecover

y N A 99. 0 97. 8 93. 3 93. 5 PFO S

Concentratio n (ng/mL ) <0.10 0 0.90 3 8.3 9 91. 2 344 %Recover

y N A 91. 7 85. 2 92. 6 69. 8 N A= No t Applicabl e Reporte d results fro m extema l standar d calibration . 

## Tabl e 41 . Rinseat e Blank s

3MUMSI D GLP10-01-02-18-133 GLP10-01-02-18-134 Descriptio n DALGW130LR B 110630 DALGW133SR B 110630 PFB S Concentratio n (ng/mL ) <0.025 0 <0.025 0 VoRecover y NA NA PFH S Concentratio n (ng/mL ) <0.025 0 <0.025 0 VoRecover y NA NA PFO S Concentratio n (ng/mL ) <0.040 0 0.047 8 VoRecover

y N A N A NA = Not Applicabl e Reporte d results torPFB S an d PFH S ar e from extema l standar

d calibration . Reporte d results fo rPFO S ar e from intema l standar

d calibration .

Pag e 3 8 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



## Laborator ycontro l spike s an d fiel d matri xspike s were use d t o determin e th e analytica l metho d accurac yan d precisio n fo rPFBS , PFHS , an d PFOS
. Analysi s wa s successfull ycomplete d followin g 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 describe d herein .

Al l remainin g sample s an d associate d projec tdata (hardcopyan d electronic) wil l b e archive d accordin g t o 3 M Environmenta l Laborator ystandar d operatin g procedures .

Attachmen t A : Protcx:o l Amendmen t 1 8 (Genera l Projec tOutline ) Attachmen t B : Representative Chromatogram s an d Calibratio n Curve s Attachmen t C : Analytica l Metho d Attachmen t D : Metho d Deviatio n

Pag e 3 9 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Date 

# 'zeJ ) Clesto n Lange,i^h.D. , 3 M Principa l Analytica l Investigato r 

# 2jf£i^r^// Willia m K . Reagen , Ph.D. , 3 M Environmenta l Laborator yDepartmen tManage rDate



# u Gar y , Spcifiso rRepresentative 

# OM/J y Date

/ Date Jaisimh a Kesari,^tud yDirecto r

Pag e 4 0 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



# ATTACHMEN TA : PROTOCO LAMENDMEN T

Pag e 4 1 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 

## Analytica l Protocol : GLPIO-01-0 2

Amendmen t 1 8



# Stud yTid e Analysi s o fPerfluorooctan e Sulfonat e (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen t fo rth e 3 M

Decatu rPhas e 3 Site-Relate d Monitorin g Progra m
PROTOCO LAMENDMEN TNO
. 1 8

Amendmen t Date : Jun e 15,201 1 

# Performin g Laboratory 3 M Environmental , Health , an d SafetyOperation s

3 M Environmenta l Laborator y Buildin g 260-5N-1 7 Maplewood , M N 55144-100 0
Laborator yProjec tIdentificatio n

GLPIO-01-0 2 Samplin g Even t Forme rSludg e Incorporatio n Are a (FSIA ) Groundwate r

Pag e 1 o f8 Page42o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 

## AnalySca l Protocol : GLPIO-01-0 2

Amendmen tI B 

# Thi s annendmen t modifie s th e followin g portio n o fprotocol : "Analysi s o fPFOS , PFH S an d PFB S i n Groundwater , Soi l an d Sedimen t fo rth e 3 M

Decatu rPhase 3 Site-Relate d Monitorin g Program "

PimTocoL READS : 

## N o change s t o th e wordin g o fth e protoco l ar e required .
AUEN D T

O READ : 

## N o change s t o th e wordin g o fth e protocxi l ar e required . Thi s amendmen tonl yaddresse s an d document s th e additio n o fth e Genera l Pnsjec t Outlin e (GPO ) fo rth e collectio n an d analysi s o fgroundwate rsample s fro m Decatur , AL , an d conducte d a s part o fth e 3 M Decatu rPhas e 3 Progra m fo rPFOS , PFH S an d PFB S (GLPIO-01-02)
. Th e anticipate d sampl e collectio n wil l occu raroun d th e timeframe durin g th e wee k o f Jun e 20,2011 . Th e groundwate rsample s fo rthi s samplin g even twil l b e entere d int o th e 3 M Environmenta l Laborator yLIM S a s projectGLP10-01-02-1 8 an d reporte d a s interi m reportGLP10-01-02 - 18 , (reflectin g stud yGLP10-01-G 2 an d amendment-18)
.
Reason ; 

## Th e reaso n fo rthi s amendmen t i s t o documen tth e Genera l ProjectOutlin e (GPO ) whic h describe s th e anticipat e groundwate rsampl e collectio n even tt o b e conducte d fo rth e 3 M Decatur , A Lfacility
. Th e GP O i s fiv e page s i n lengt h an d include d a s attache d t o thi s amendmen tform
.

Pag e 2 o f8 Pag e 4 3 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1 

## Analytica l Protocol : GLPIO-01-0 2

Amendmen t1 8 

# Amendmen t Approva l 

# J^ HW^1^^ . % , 

# ( ( Gar yHohenstein , Sponso rRepresentative 

# ^/^s/ , 'Z.C) } Clesto n C
. Lange , Principa l Analytica l Investigato r Date

^ ^ ^ 

# /j-Jz// ^ ^O/ J Willia m Reagen , EH S Opn s Environmenta l La b Managemen t 

# iM.A/ / Dat e

Jaisimh a Kesar i P.&rDEE , Stud yDirecto r

Pag e 3 o f8 Page44o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 

## Analytica l Protocol : GLPIO-01-0 2

Amendmen t1 8 

# Environmenta l Healt h & Safet yOperations , Environmenta l Laborator y

Genera l Projec t Outlin e To : Gar

yHohenstein , 3 M EHS&Opn s From : Susa n Wolf , 3 M EHS&Opns ; Environmenta l La b cc : Willia m Reagen , 3 M EHS&Opns ; Enwonmenta l La b

Clesto n Lange , 3 M EHS&Opns ; Environmenta l La b Ja i Kesari , Westo n Solution s Date : Jun e 15,201 1 Subject : Analysi s o fPerfluorooctan e Sulfonat e (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d

Perfluorobutan e sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen tforth e 3 M Decatu r Phas e 3 Site-Relate d Monitorin g Program ; GL P Interi m Repor t1 8 - Fonne rSludg e Incorporatio n Are a (FSIA ) Groundwate r- Jun e 201 1 Samplin g 

# 1 Genera l Projec t Informatio n

Contact s

1 La b Reques t Numbe r 1 Si xDigi t Departmen t Numbe r 1 Projec t Scheduien'M tDate s 3 M Sponso rRepresentativ e Gar yHohenstei n 3 M EH S Operation s 3 M Buildin g 224-5W-0 3 Sain t Paul , M N 55144-100 0 Phone:(651)737-357 0 aaliohenstein(S)mmm.co m 3 M Envirormtenta i Laborator yManagemen t Willia m K . Reage n 3 M EH S Opns
. Environmenta l l.at}oratofy 26(WN-1 7 65 1 733-973 9 vi*reaqen(a)mmm.co m Principa l Analytica l Investigato r aesto n Lang e 3 M EH S Opns , Environmenta l Laborator y 26(«N-1 7 65 1 733-986 0 cclanQe(3)mmm.co m Samplin g Coordinato r Timoth yFrina k Wissto n Solution s Timothv.frinak<aiwestonsolutions.co m Phone : (334)-332-912 3 GLP10-01-02.1 8 Dep t #530711 . Project #002267444 9 Samplin g schedule d fo rth e weel < o fJun e 20,201 1 

## Al l verba l an d writte n correspondenc e wil l tt e directe d t o GaryHohenstein .

Pag e 4 o f8 Pag e 4 5 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 

## Analytica l Protoa>t : GLP10-01-0 2

Amendmen t1 8 

# 2 Backgroun d Informatio n an d Project Objective(s ) Th e 3 M EH S Operation s Laborator y(3 M Environmenta l Lab ) wil l receiv e an d analyz e gn>und\ivate r sample s collecte d fro m twenty-seve n groundwate rwell s fo rPerfluorobutanesulfonat e (PFBS) , Perfluorohexanesulfonat e (PFHS) , an d Perfluorooctanesulfonat e (PFOS ) fro m th e Fomie rSludg e Incorporatio n Are a (FSIA)
. Analyse s wil l b e conducte d unde rth e GL P requirement s o fEP ATSC AGoo d Laborator yPractic e Standard s 4 0 CF R 792 . Groundwate rsample s fro m Decatur , A Lwil l b e collecte d byWesto n Solution s personne l th e wee k o fJun e 15,2011 . Th e 3 M Environmenta l Laborator ywil l prepar e th e sampl e bottle s wit h al l require d spike s t o ensur e tha t result s fo rPFBS , PFHS , an d PFO S ar e o fa know n precisio n an d accuracy . Th e fina l report wil l b e submitte d t o Gar yHohenstei n an d Ja i Kesar i upo n completio n unde rinteri m repor tGLP10-01-02 - 18 . 

# 3 Project Schedul e Sampl e collectb n bottle s wil l b e prepare d b y3 M Environmenta l Laborator yfo rsamplin g th e w^ co fJun e 20 , 2011 . Sampl e bottle s wil l b e shippe d i n cooler s ovemigh tt o 3 M DK:atu rfo rarriva l o n Friday , Jun e 17,2011 . Sampl e bottle s shoul d b e store d refrigerate d on-sit e unti l sampl e collection . Marti n Smit h \

Westo n Traile r 3 M Decatu rPlan t 140 0 Stat e Dock s Roa d Decatur , Alabam a 3560 1 

# 4 Test Parameters Th e targete d limi t o fquantitatio n wil l b e 0.02 5 ng/m L(ppb ) fo rPFBS , PFHS , an d PFOS
. Atota l o fttiirty-one samplin g kxation s have bee n specified
. Fo reac h samplin g tocation, a sample , sampl e duplicat e an d a minimu m oftwo fiek j matri xspike s wil l b e collected . Tabl e 1 indicate s th e wel l I D an d correspondin g fiel d spike levels . Th e lil l t o here ° lin e o n eac h 25 0 m LNalgen e botO e wil l b e 20 0 mL . Tw o set s o ftri p blank

s consistin g o freagent-grad e water , a low-leve l tri p blan k spike , mid-l&« l fid d spike , mid-hig h fiel d spike , an d a high-leve l tri p blan k spike wil l b e prepare d a tth e 3 M Environmenta l Laborator yan d sen tt o th e samplin g k>catio n wit h tiie othe rbotU ^ Tw o addffionatbotUe s wil l b e prepare d t o b e use d fo rth e preparatio n o fth e ^uipmen t rinseate blanks . A 1 - Lcontaine ro flaborator yreagen twate rwil l b e provkJed fo rth e collectk> n o fth e equipmen t rinseate blanks . Base d o n th e result s fixan previousl ycollecte d sampte s fro m tiie FSI A(GLP10-01-02-O7 an d GLP10-01-02-11) , most sampRn g bcation s wil l require dilutio n wit h latxuaiDi yreagen twate rprio rt o sampl e aredysis . However , samplin g kxstion s identifie d i n S ^ 1 o fTabl e 1 ar e expec tt o contai n PFBS , PFHS , an d PFO S concentration s <1 0 ng/m LFo rsample s collecte d firo m tiiese samplin g locations , tiie sample , sampl e duplicat e an d fiel d matri xspike s wil l contai n th e additio n o finterna l standard '"Oz-PFBS , "Ca-PFHS , an d ^^CVPFOS , a ta nomina l concentratio n o f1 ng/m La s wil l th e tri p blank s samples , tri p blan k

lowspik^ , an d tw o equipmen t rinseate blanks . Th e additiona l o fintema l standard s wil l b e use d t o ak i m th e analysts o fth e sampiss . Atote l o f 16 9 sampl e bottie s wil l b e prepared .

Pag e 5 o f8 Pag e 4 6 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1 

## AnalyUca l Protocol : GLPIO-01-0 2

Amendmen t1 8 Tabl e 1 . Samplin g Location s an d Fiel d Matri xSpik e levels . Set # 1 2 3 4
5 Wel l No . 134S , 135S
. 136R , 137R , 137S
. 137Land138 L 130L . 131S , 131L , 133R , 135L.136S , 136L,141R , 142R . 143 R an d 144 R 130R , 130S , 133S , 133L . 134L , 138R , 138S , CW26C
. CW26 Lan d 140 R 131R , 134Rand136 R Tri p Blan k

Set s 1 an d 2 Sampl e Leve l Lo w Mk l Hig h Lo w Mi d Hifl h Lo w Mk i HkJ h Lo w Hig h l-O W 

## Mi d Mid-Hiq h Hig h Spik e Cone
. (ng/mL ) 0. 1 1. 0 1 0 1 1 0 10 0 1 0 10 0 50 0 10 0 200 0 1 1 0 10 0 50 0 

# 5 Tes t Metiiod s Sample s wil l b e prepare d an d analyze d byLC/MS/M S foltowin g ETS-8-044. 0 "Detemninafio n o fPerfluorinate d Compound s I n Wate rb

yHig h Perfonnance Liqui d Chromatography^as s Spa^bnomeb yDirec tinjectio n Analysis' . Th e dat e qualityobjective s fo rtiiese studie s ar e quantitetive result s fo rtiie targe tanalyte s witt i a n analytica l accurac

yo f100±30% . Rek

J matri xspke s notyieMin g recoverie s withi n 100±30 % wil l b e addresse d i n ttie reportan d tiie fina l accurac ystatemen tma

yb e adjuste d accordingly . Base d o n tiie results fro m previousl ycollecte d sample s fio m th e FSI A(GLP10-01-02-07) , mostsamplin g kx:ation s wil l require dilutio n witt i laborator yreagen t wate rprio rt o sampl e analysi s byETS-8-044.0
. Fo rsample s conteinin g intema l stendard : sample s wil l b e analyze d agains ta n intema l standar d calibratio n curve . Eac h curve poin t wil l contai n isotopically-labeie d perfluorocart}Oxyii cackJ s an d perftuorosutfoni cacid s a t a nomina l concenb^atio n o f1 ng/mL
. Th e calibratio n curve wil l b e generate d bytakin g ttie rati o o ftt^ stendar d pea k are a counts ove rttie intema l standard pea k are a oounte t o fi ttt« dat e fo reac h analyte . Alternately , sample s ma

yb e analyze d byET S 8-154. 3 "Detenminatic« i o fPerfluorinate d Adds , Akxjhols , Amktes , an d Sulfonate s I n \A^te rB ySoli d Phas e Extiactio n an d Hig h F^erfbmianc e LK|uk ] Chromatography/Mas s Spectiomety " o rETS-8-110. 1 "Mettw d o fAnalysi s fo rttie D^arminatio n o f Perfluorinate d Compound s i n V\^ter , Soi l an d Sedimen tb yLC/MS/MS*
. 

# 6 Reportin g Requirement s Fo reac h samplin g location , ttie reportwil l contai n ttie resutts fo rttie sample , sampl e duplicate , an d fiel d nnatrix spikes . Tri p blan k

an d tiip blan k

spike s wil l b e reported fo rth e samplin g even ta s wi Q an yequipment/rinseat e blank s prepare d i n th e field
. Laborator ycontro l spike s o freagen twate rprepare d at th e time o fsampl e preparatio n wil l als o b e reporte d an d use d to e\raluate ttie overal l mettKx J acoirac yan d predsion . M^o d blank s o freagen t wate rprepare d a t ttie time o fsampl e preparatio n wi B b e use d to d^ermin e ttie mettio d detectio n limi t Fo rthos e samplin g kx^tion s wtier e th e fieki matri xspic e \&im \ wa s no tappropriate , du e to highe rtha n expect s anatyt e concentrations , a laborator ymatri xspike ma

yb e prepare d mx i wil l b e include d i n tiie final report.

Pag e 6 o f8 Pag e 4 7 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1 

## Analytic ^

Protocol : GLP10-01-0 2

Amendmen t 1 8 

# 7 Emai l Correspondenc e Quarterl yG Wsamplin g - Decatu rForme rSludg e Incorporatio n Are a Well s Young , Charle s T . to : stwotf@mmm.co m 06/13/201102:1 4 P M Cc : "Frinaic , Timoth yR.'" , "Smitii , Martin " Sue : WESTO N wil l b e performin g quarterl ygroundwate rsamplin g o fth e forme rsludg e incorporatio n are a (FSIA ) well s beginnin g o n Jun e 20,2011 . Th e attache d wor d documen t show s th e FSIAwell s (highlighte d i n yellow ) tha t wil l b e sample d fo rfluorochemica l analyses-unde rGLP 10-01-0 1 an d GLP 10-01-02 . I t als o show s samplin g schedul e fo r othe rquarterl ysamplin g (an d conventiona l parameters ) /^s o attache d i s a spreadshee t summarytabl e fro m th e mos t recen t (Nov-2010 ) roun d a t th e FSIAwells . I n additio n t o th e primary/duplicate/spik e bottles , w e wil l nee d bottle s an d Milli- Q wate rfo rth e tw o equipmen t rinseate blanks tha t wil l b e collected . Thanks , Charli e Charie s T . Youn g Technica l Directo r Westo n Solutions , Inc . 140 0 Westo n Wa y(5-2 ) Wes t Chester , P

A1938 0 (610)701-378 7 Fa x(610 ) 701-340 1



## Pag e 7 o f8 Page48o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 

# Analytica l Protocol : GLPIO-01-02

Amendment 1 8 QuarteriyG

WMonitorin g 1 Rrs t an d Thir d Quarte rIMonitorin g Progra m 201 L 203L * 204 L 206 L 209 L 211 R 212 R 212 D 213 R 213 S 215 R 215 D

216 R 217 R 217 S 217 D 218 R 218 L 220R * 220L * 221 R 222R * 223 R

226 R 225 L 226 R 226 L 227R * 227L * 228 R 228 L 229 R 229 L 306 R

307 L 308 R 310R * 311 R 313 R 315 R 317L * 319 L 324L * 325 L 328R *

328L * 330R * 330L * 332 S 335R * 50 1 50 3 50 7 510 R 51O L 511 S 1

512 R 512 L GRS04* ^ RW312R * RW327R * RW331S *

Secon d an d Fourt h Quarte rMonitorin g Progra m 201 L 203 L 211 R 212 R 212 D 213 R 213 S 217 R 217 S 217 D 218 R

218 L 222 R 223 R 22g R 229 L 305 R 307 L 313 R 315 R 324 L

325 L 332 S 335 R 512 R 512 L 130R* * 130S* * 130L* * 131R* * 131S* *

131L* * 133R* * 133S* * 133L* * 134R* * 134S* * 134L* * 135R* * 135S* * 135L* * 136R* *

136S* * 136L* * 137R* * 137S* * 137L* * 138R* * 138S* * 138L* * C\A/26C** CW26L* * 140R* * 1

141R* * 142R* * 143R* * 144R* *

Monitorin g well s wil l b e analyze d forVOC s an d isopfopy l efth ^ usin g EP AMetho d 826 0 unles s othenMis e indica ^ O Monitorin g well s wil l b e analyaa l fo rPFO Aan d VOC s / isopropy l ethe rusin g EP AMelho d 8260 . ( ^ Monitorei g well s wil l b e analyze d fo rPFO A "On e sampl e wil l b e collecte d fro m th e ctose d landfa i (SWVMU4) groundwate r«clraclk> n syste m forVO C an d PFO A analysis . R Windicat e recover ywell s use d i n activ e gromdwate rextractio n systems .


# Pag e 8 of8 Pag e 4 9 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1


# ATTACHMEN TB : REPRESENTATIV E SAMPL E CHROMATOGRAM S AN D CALIBRATIO N CURVE(S )

Pag e 5 0 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Workstation : ETSKIR K Result s Name : kll0720a.rdb 

### k110720a.rd b (PFBS) : "Quadratic " Regressio n (" 1 / x " weighting): y= -4.75e+00 3 x'* 2 + 1.39e+00 6 x+ 3.69e+00 3 ( r= 0.9999 )

1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 Concentration , ng/m L 

## 6 5 7 0 7 5 8 0 

## 8 5 9 0 9 5 10 0 Dat aworke du p b y KJ U Pag e 1 o f 1 Printin g Time : 2:05:5 8 PM Printin g Date : Wednesday , Jul y27 , 201 1 

### Pag e 5 1 O f 11 3GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Workstation : ETSKIR K Result s Name : kll0720a.rdb

Dat a worke d up byKJ U Pag e 1 o f 1 Printin g Time : 2:07:5 4 P M Printin g Date : Wednesday , Jul y27 , 201 1 Pag e 5 2 O f 11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Workstation : ETSKIR K Result s Name : kll0720a.rdb



### 4 5 5 0 5 5 Concentration , ng/m L Dat aworke du p b y KJ U Pag e 1 o f 1 Printin g Time : 2:07:1 9 PM Printin g Date : Wednesday , Jul y27 , 201 1 

### Pag e 5 3 Of 11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS
. PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b 

# I Sompt B Namo : 1(1107208018 - Sampt o ID : •11003-26* " Paa k Namt : "PFBS ' Masqat) : *299.000/80.00 0 Oa.299.0D l CommeM:-2. 5 r>9/mLFCd( J I n Mil l Q Water - Annotation : " Sampl eType ; Standar d Concentration : 2.5 0 ng/m L Calculate dCone : 2.5 1 ng/m L 7/20/201 1 3:17:0 5 P

M Modified : Proc . Algorl t

•r.tag e Base . Sub . Peak-Split . Facto r Repor t Larges t

Pea l Min . Pflak

Height : Min . Pea k

Mldth : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT :

350385 4 CO

L 6.38et00 5 13. 0 13. S 5.2a 5 5.0e S 4.8« S 4,6« 5 4.4a 5 4.2e 5 4.0« 5 3 * « 3.Se 5 3.4« S 3.2* 5 3,0«5
- 2.8* 5 • 2.6a 5 2.4« 5 2.2a 5 2.00 5 1.8* 5 1.6*5
- 1.4*5
- 1.2*5
- 1.0*5
- 8.0* 4 6.0*4
- 4.0*4
- 2.0*4
- 0.0
- 12. 0 12 5 13f l 13. 5 

# I Sampl a Nam* : M 10720a028 - Sampl * 10 : 'Solven t Blani rFile : 1(110720a.w< r Peal ( Name:-PFBS - M«w(ea):-299.000/80JX»Da.299.00(V99.000i3a CommentTX«QV\UerTN11-0238 ' Anr>otatton: - Sampl eType : 60 0 :<jl a Acq . Dat e Acq . Tim e Modified : Proc . Algorithm : Noir ePercentag e Base . Su b Peak-Spl l 7/20/201 1 6:4 e 2 6 P

H Ko Spe c Window :

. Fac t Repor t Larges t

P Min . Pea k Min . Pea k Smoothin g R TWindo w Heigh t

Width : Width : Expecte dRT : Us eRela t Int . Typ e Retentio n Area : Height : v eRT :

Time : eak :

3 0 1 fy P a 5 0 1.0 0 2 Ye s 0.0 0 0.0 0 0 0

. : n M o Bas e 301 3 T o E a •^.STe+OO : c

11. 0 

# I SampteName:"V110720a029 - SamptelD:-LCS-110714- r Paa k Name : 'PFBS - Massfee) : -299^)00/80.00 0 Da.299.00 a Comment : "O.Spp b LCS ' Anrxitatlon : " Sampl eIndex : 2 9 I San ^ Name : 1(110720803 7 Samp4etD:-LCS-110714-4 - t^le:-k110720a.wt r P*al ( Name : TFBS - Ms3s(es} : -299.000/80.00 0 Da,299.000/99.00 0 Da ' I CommenfSpp b LCS * Anrmtation : " ampl eIndex : 3 2 Sampl eType : Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Wind o O C 0.19 8 0.50 7 7/20/201 1 ng/m L

ng/m L 7:07:2 1 P

M Ye s Specif y P SO * : 1.0 0 Peak-Split . Factor : 1 Repor t Larges t

Peak : Ye s Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : camet e ml n c p s 0.0 0 se c 0 30. 0 13. 2 N o Manua l 13. 2 Area : 70752 0 c o Height : Star t Time : .086+00 5

13. 0 a e o

mii ^

c p s 1.05* 5 1.00e 5 9.S0e4 ' 9.00e4
- 8.S0e4
- 8.00* 4 7.50* 4 7.00e4 : 6.50*4 : e6.00*4
- I5.00»4
- 4.50*4
- 4.00*4 : 3.50*4
- 3.00*4
- 2.50*4
- 2.00*4
- 1.50*4 ^ 1.00*4
- 5000.00 ^ 0.00
- Sampl aTyp e Concontrati o Calculate di Acq . Time : Modified : Proc . Algor : Nois ePerce n Base . Sub . h Peak-Split . Repor t Larg e Kin . Pea k

He Q

C 1.9 8 5.1 1 7/20/201 1 8:10:1 2 P

M No Specif y Pa r 5 0 Indow : 1.0 0 Fac t r : 2 S t Pe4k : Ye s Igh t Kin . Pea k

Width : Smoothin g W

: R TWindow : Expecte dR

T Us eRelativ e Int . Type : Retentio nT i Area : Height : dth :

RT : 0.0 0

0.0 0 30. 0 3 13. ? m N o Valle y 13. 2 . 702108 8 cou n .07e+00 6 c nq/m L

ng/m L

rain c p s p o I n

p s 1J)5e 6 1.00* 6 9.S0eS
9.00* 5 850* 5 8.00*5
- 750* 5 7.00* 5 650* 5 6.00* 5 o 5.50* 5 1 5.0OB 5 4.50e 5 4.00e 5 3.S0a 5 3.00a 5 2.50 e 2.00* 5 1.50*5 ' 1.00* 5 5.00* 4 15 ^ 1S. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 0 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 o f 2 9 

### Pag e 5 4 O f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# I Sampl * Name : >110720*035 " Sampl e 10 : •LCS-110714- rFl* : l(11D720a.wii r Paa k Name : "PFBS " Man(as) : *299.00Q/80.00 0 Da,299.000i/99.00 0 Da ' Commert : *30pt3f

a LCS * Annotation : - Sampl e Index : 3 5 Sanipl e Type : Q C Concentratlor, ! 29. 9 Calculate d Cone : 29. 9 Acq . Date : 7/20/201 1 Acq . Time : 9:13:0 6 P M Modified : Ye s Proc . Algorithm : Specif yPa Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 Peak-Split
. Factor : 2 Repor t Larges t Peak : Ye s Min . Pea k

Height : 0.0 0 Mln . Pea k

Width : 0.0 0 Smoothin g Width : 0 ng/m L ng/m L

a met e ml n cp 3 p o

Retentio n Area : Height : Star t Ti m En dTime : T me : 1 3

"3734469 1 5.63e t 1 2 1 3 7 10 6 0 a

11. 0 12. 0 12. 5 13. 0 

# I Sampt * Nam* : 1(110720*045 - Sampl e iD:-LCS-110714-i rFile:lc110720a.w« r P*a KNama : 'PFBS - Masses) : -2d9.OOCV60.000 Da.299.000if99.00 0 Oa " Comm*nt : "Spp b LC S EC P Annotation : " Sampl e 

# I Sampl e Name:l(110720a04 7 Sampl e ID:*LC5-110714-1f rFile:-k110720a.wt r Pea k Name:-pFBS - Massfea):-299.000/80JXIODa.299.000/9g.00 0 Da Commen t •0.5pf * LC S Ec rAnnotalkin ; ~ Sampl eIndex : 1 2 Sampl eType : Q C Concentration : 0.0 0 ng/m L Calculate dCone : 0.0037 7 ng/m L Acq . Date : 7/20/201 1 Acq . Time : 11:39:5 3 p « Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Windov . Peak-Split . Fact e Repor t Larges t

P e Mln . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindov : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Area : Height : Star t Time : En dTime : N o Specif y P a 5 0 : 1.0 0 r : 2 0.0 0 0.0 0 0 30. 0 13. 2 N o Valle y 13. 2 892 3 co u .55e+00 3 13. 0 13. 3 n

11. 0 

# I Sampl e Name:l(110720aO48 - Sampl e ID:-LCS-110714-1S ' File:l(110720a.wi r Pea k Name : "PFBS - Ma39<*a) : -299.00<VS0.00 0 Da.299.000/99.00 0 Da Commen t "SOpp b LC S EC rAwKrtaUon : - Sampl eIndex : 1 8 Sampl eType : Q C Concentration : 0.0 0 ng/m L Calculate dCone : 0.32 3 na/m L6.5*4
- Acq . Date : 7/21/201 1 rims : 1:15:1 1 A M 6.0*4
-

s - M Q II I 5.5* 4

5.0*4
- 4.Se4
- 4.0e4
- I3.5*4
- 

# I I 3.0e4

- Z5*4 - 2.0*4 - 1.5*4 - 1.0*4 - Sampl o Type : Q C Concentration : 0.0 0 n g m L Calculate dCone : 0.053 9 ng/m L Acq . Date : 7/21/201 1 Acq . Time : 12:42:1 9 A M Modified : N o Proc . Algorithm : Specif y Para m Mois ePercentage : 6 0 Base . Sub . Window : 1.0 0 m Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s ter s n Min . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 R TWindow : 30. 0 se c Expecte dRT : 13. 2 mi n Us eRelativ eRT : N o Retentio nTime : 13. 2 ml n Height : 1.17e+0O 4 cp s Star t Time : 13. 0 ml n En dTime : 13. 5 ml n - M Q II I 1.10e4
-

1.05*4
-

9000. C 1
-

7500.00
- 7000.00
- £:6000.CO : 1 5500.00 :

~ 5000.00 : 4500.00 : 4000.00 : 3500.00 ^ 3000.00 ^ 2500.00- : 2000.00 ^ 1500.00- i 1000.00- ^ 500.0 0 j 0.0O J Acq . Tim s Modified : Proc . Algorithm : Nois ePe r Base . Su b Peak-Spl i Repor t L a Min . Pea k Min . Pea k R TWindo w rentage :

Windo w

. Fact o ges t P e Height : Width : Width : Expecte dRT : Us eRela t int . Typ e Area : Height : v eRT :

Time : 1 5 6 Star t Time : En dTime : :15:1 1 No Specif y A

M P a 1.0 0 r : 2 k : Y 0 0 30. 0 13. 2 No Valle y 13. 2 66 2 69e-^0 0 12. 9 13. 1 (1 0 fl O

Dat aprinte db y ST

W Printin g Time : 12:06:0 0 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 o f 2 9 201 1 

### Pag e 5 5 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b 

# I Sampi*Name:l(110720a06 rSampl a iD:-GLP1(W)1-02-1WXW " File : 1(110720a. w Peai t Name : -PFBS ' Maaa(ea) : -299.000/80.00 0 Da,299.000/99.00 0 Da Commont:-DALGW130R0110630((iai:100) - AnnotaUon: - Sampl e Index : 6 1 Sampl e Type : Unknow n Concentration : N/A Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentage : Base . Sub . Windo * Peak-Split . Fact o Repor t Larges t

P e Mln . Pea k

Height : Min . Pea t

Width : Smoothin g Width : R TWindow : Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : 16. 1 7/21/201 1 6:18:0 6 A

M No ng/m L
Specif y Parameter s 5 0 : 1.0 0 r : 2 a k : Ye s 0.0 0 0.0 0 0 30. 0 a 13. 2 m No Valle y 13. 2 m Area : 23187 9 cou n Height : 2 Star t Time : En dTlmo : mi n c p s s e c poin t n

n s .52e«00 4 cp s 12. 9 m 13. 5 m n

h - M

Q II I

2.0* 4

1.8* 4

1.0* 4 aooo. 0 6000. 0 4000. 0 2000. 0 0. 0 11. 0 •,'"S"i-'|- | ," 1 1 '• (r ^ 12. 0 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 

# I SafiH)l*Mame:l(110720a07S - Sampl e iD:-GLP1tM)1-02-18-016 - File:lt110720a,wi r Peal t Name : -PFBS ' M8*s(es) : -299.O00A0.00 0 Da.299.000/99.00 0 Da ' Commert:"DALGW130L0110630(cIi|l:10) " AnFiotaBon: " Sampl e Index : 7 5 Sampl e Type : Unknow n Concentration : N/A Calculate d Cone : 1.6 8 ng/m L^e» ^ Acq . Date : 7/21/201 1 3.6*4
- Acq . Time : 11:11:5 2 A M 3.4*4
- Modified : Ye s •Sampl e Name : l(110720a070 - Sampl e ID : 'GLPI041-02- 1 frOI V File : -i(110720a.wi r I Peal t Name : 'PFBS - Ma3a(*s) : -299.000/80.00 0 Da.299.O0CV99.OO O Da - •Comment-DALGW130SD110630{di)1:10) - AnnoUtlon: - Sampl e Index : 7 0 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 3.& 3 ng/m L Acq . Pate : 7/21/201 1 Acq . Time : 9:27:0 2 A M Modified : Proc . Algorithm : Nois e Percentage : Base . Sub . Windo w Peak-Split . Fact o Repor t Larges t

P e Min . Pea k

Height : Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : Ye s Specif y 5 0 Parame t 1.0 0 mi n r t 3 - k : Y

« 0 0 0 30. 0 13. 2 No Manua l 13. 2 Area : 49365 9 c Height : 7 Star t Time : En dTine : 59et00 ' 12. 9 13. 1 0 0 c p 0 0 s e ml n

ount s c p s ml n mi n 7.5* 4 7.0* 4 6.5* 4 6.0* 4 5.5* 4 5.0* 4 4.5* 4 4.0e 4 3.5* 4 3.0* 4 2.5* 4 Z0»4 ' 1.5* 4 1.0* 4 5000. 0

- i

11. 0 11. 5 IZ O 12. S 13J ) 13. 5 14. 0 14. 5 15. 0 15. 5 

# I Sarr^Nam*:l(110720a08 rSampl a ID : *&LP1CM}1.Q2.1M)25 ' FDa : I d 10720a.wi r Pea k Nam* : *PFBS - Mass{*a) : -299.00a'80.00 0 Da,299.000199.00 0 Da ' Convn*nt-DALGW131S0110622(ciai:10) * Annotation: - Sampl e Index : 3 8 Sampl e Type : Unknow n

Proc . Algorithm : Specif y Nois e Percentage : 5 0 Para m Base . Sub . Window : 1.0 0 m Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0 Mln . Pea k

Width : 0 Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 13. 2 Us e Relativ e RT : No Int . Type : Manua l Retentio nTime : 13. 2 Area : 23702 3 c Height : 3.75e+00 . Star t Time : 12. 9 t e r n 0 0 cp s 0 0 se c ml n

ount s op a ml n p o l - M

O II I

t s 

## % 3.2e 4 3.0* 4

Z4* 4 2.2* 4 2.0* 4 1.8* 4 Calculate dCone : 0.91 1 na/m L Acq . Date : 7/21/201 1 Acq . Time : 3:14:4 5 P M Modified : Ye s Proc . Algorithm : Specif y Parameter s Nois e Percentage : 5 0 Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 3 Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se e Smoothin g Width : 0 pol n R TWindow : 30. 0 Se c Expecte dRT : 13. 2 ml n Us e Relativ e RT : No Int . Type : Bas e T o Bas e Retentio nTime : U. 2 ml n Height : 1.91e+00 1 cp s Star t Time : 12. 9 ml n - M Q II I



## J f 1.7* 4 1.6e 4 1.5e 4 1.4e 4 1.3* 4 1.2*4 ^

1.2* 4 1.0* 4 8000. 0 6000. 0 4000. 0 2000. 0 9000.0 ' 8000. 0 7000.0 - 6000.0 - 5000.0 - 4000.0 -

0, 0 ViW'^UJ»(M**fiv,>u*'44i^.iii i iWiyw i 13.07.^ 1 iaijUalJ^^HUuLUL«u.M u 14. 0 14. 5 15. 0 15. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 0 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e3 o f 2 9 

### Pag e 5 6 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

I Peal t Nam* : -PFBS " Mas9(es} : '299.000/60.00 0 Da,299.000/99.00 0 D a Sampl e Index : 9 7 Sampl e Type : Unknow n Concentration : N/ A7=- ^ Calculate d Cone : 3.1 0 ng/m L''^^ ^ Acq . Date : 7/21/201 1 Acq . Time : 6:53:1 7 P M yQ ^ Modified : N o Proc . Algorithm : Specif yParameter s - M Q II I 65* 4 Nois e Percentage ! 5 0 Base . Sub
. Window : 1.0 0 ml n Peak-Split
. Factor : 2 6.0* 4 Repor t Larges t Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se c5.5* 4 Smoothin g Width : 0 point s R TWindow : 30. 0 se c Expecte d RT : 13. 2 mi n 5.0* 4 Us e Relativ e RT : N o Int . Type : Valle y*-5« 4 Retentio n Time : 13. 2 ml n * Area : 17622 9 count s g " . ^ Height : 7.fi2e*O0 1 cp s > : '"" " Star t Time : 12. 9 mi n S En d Time : 13. 1 ml n J 35 ^ 3.0* 4 2.5* 4 2.0* 4 1.5* 4 1.0* 4 5000. 0
11. 0 li. 5 12. 0

•Ssii<pleName:i(110720a111 - Sampl e lD:'GLhMI1-02-1&O40 - t^ll e 1 I Pea k

Nam* : -PFBS - Mass<es} : "299.000^0.00 0 Da.299.00W99.00 0 Da " •Comm*nt:-0ALGW133S0110630(dil1:10) - Annotation: - Sampl e Index : 11 1 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 9.0 1 ng/m L^-^ ^ Actj . Date : 7/21/201 1 Actj . Time : 11:17:5 1 P M 3.6* 4 Modified : N o 3,4«4 . Proc . Algorithm : Specif yParameter s - M O II I Nois e Percentage : 5 0 3 3* 4 Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 2 Repor t Larges t Peak : Ye s o.u*^ ' Mln . Pea k

Height : 0.0 0 cp s Mln . pea k

Width : 0.0 0 se c2-6*4 - Smoothin g Width : 0 point s R TWindow : 30. 0 se e 2.6*4 - Ej;pecte d RT : 13. 2 mi n Us e Relativ e RT : N o 24*4 - Int . Type : Valle y Retentio n Time : 13. 1 mi n « ^-J*4 - Area : 25101 6 count s fr Height : 3.B8e»00 1 cp s ^ 2.0*4 - Star t Time : 12. 9 ml n 1 1 En d Time : 13. 3 ml n % 1.8*4 - 1.6*4 - 1.4*4 - 1.2e4 - 1.0*4 - 8000.0 - 6000.0 - 4000.0 - 2000.0 - Tc110720a.wlf r

11. 0 1l'. 5 12. 0 13.1 6



# 1 

# 1 

# i

12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5

1 3 1 4



# 1 

# 1 • 

# A

12 5 13, 0 13. 5 14. 0 14. 5 15. 0 15. 5 l"sa-;;?;Tl'a-;;;;"-k110720a1i yS«mp>;ii5r^LPiMi--0gltU)45 - File:-V110720a.wt r"^ ^

' ~"" ^ ^ I Pea k NarDe : "PFBS " Mas»{e«):-299.00080.00 0 Da.299.000»9.00 0 Da ' •Comment-DALGW133R0il0630(dU1:10) - Annotatton: - Sampl e Index : 11 6 : Sampl e Type : Unknow n ,3, . concentration : N/A^ „ , j |" * Calculate d Cone : 4.7 0 ng/m Li.uuoa- i Acq . Date : 7/22/201 1 i Acq . Time : 1:32:3 5 A M 9.50*4 ^ Modified : N o 9.00* 4 Proc . Algorithm : Specif yParameter s - M Q II I Nols o Percentage : 5 0 850* 4 Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 2 8.00* 4 Repor t Larges t Peak : Ye s Min . Pea k

Height : 0.0 0 cp s 750* 4 Min . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s T/WW J R TWindow : 30. 0 se c'•"* * Ss^RetatW e RT : Ko" ' "' " 6.50e 4 Int . Type : Valle y6.00e 4 Retentio n Time : 13. 2 ml n « Area : 65657 2 count s g " 5.50* 4 Height : 1.02et00 5 cp s > ; Star t Time : 12. 8 mi n S 5.00* 4 En d Time : 13. 4 mi n g •E 4.50* 4 4.00* 4 3.50* 4 3.00* 4 ZS0e 4 Z00e 4

•1.50* 4 1.00e 4 5000.0 0 0.0 0 

# 1



# 1 

# 1 1 

# 1 

# J

11. 0 li. 5 12. 0 li s 13. 0 13. 5 14. 0 14. S 15. 0 15. 5 j 1 SampteNam*:'V110720a102 - Sampl e ID : •GU'iai1^-1(W35 - Fil e Trt 10>20a.«« r I Pea k Name : -PFBS " MBSs{es) : -299.000/BO.OOO Da,299.000/99.00 0 Da " •CorwnBnfDALGW133L0110630(<«1:10) " Annotation: " Sampl e Index : 10 2 Sampl e Type : Unknow n ni c concentration : N/ A,4^5 , ' V Calculate d Cone : €.6 8 na/m L' 1 1 Acq . Date : 7/21/201 1 \ 1 Acq . Time : 8:38:5 2 P M 1 3e5-^ 1 Modified : Ye s Proc . Algorithm : Specif yParameter s - M Q II I ^ Nois e Percentage : 5 0 '"* ' Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 1 Repor t Larges t Peak : Ye s ''" • Mln . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width ; 0.0 0 se c Smoothin g Width : 0 point s '•'*= • R TWindow : 30. 0 se c Expecte d RT : 13. 2 mi n Us e Relativ e RT : N o 9-0e4 - Int . Type : Bas e T o Bas e Retentio n Time : 13. 2 mi n - 8.0*4 - Area : 93011 7 count s 9 Height : 1.42e+00 S cp s > ; Star t Time : 12. 9 mi n I I 7.0e4 - En d Time : 13. 6 rain | 6.0* 4 5.0* 4

' 4.0*4 - 3.0*4 - 2.0 e - 1.0e4 - 

# 1

1 

# 1 • 

# i

IIJ ) 11. 5 IZ O 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e4 o f 2 9 

### Pag e 5 7 O f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# r SamplaName:"k110720a084 - Sampl e ID:-GLP1(>O1-02-1fr421 ' Pea k Name : 'PFBS - Mass<ea) : -299.000/80.00 0 Da,299.000/99.00 0 Da Comment : -DA LG W131 R 0 11062 2 (d B 1:1M) - Annotation: " Sampl eIndex : . 8 1 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 53. 9 ng/m L Acq . Date : 7/21/201 1 Acq . Time : 2:20:4 4 P M Proc . Alq c Nois ePer < Base . Sub . Peak-spli t Repor t La i Mln . Pea k Smoothin g Mldt l R TWindow ; Us eRelativ eRl No lecif y Parameter s - M O II I

. Factor :

12. 5 13. 0 13. 5 

# I San^Name:'V110720a129
- Sampl eID : Pea k Name:-PFBS
- Mas9(es):-299.000/80 . Comment : "DA

LG W134 L011062 8 (d a1:10) ' Sampl eIndex : 12 9 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 11. 4 ng/m L Acq . Date : 7/22/201 1 Acq . Time : 5:05:1 4 A M GLPtfrO I -Oa- 1 &054 " File :

.00 0 Da.299.000/99.00 0 Da ' Annotatton: " 

# I S«mpleNam*:T<110720a125
- Sampl eID : "GLP1CM)1-02.1W>50
- File : •k110720a.wi r Pea k Name : -PFBS " Ma3s{ea) : -299.000^0.00 0 Da.299.000/99.00 0 Oa ' Comment'DALGW134R0110628(dai:100) ' Annotathm:
- Sampl eIndex : 12 5 Sampl eType : Unknow n N/ A Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e 51. 7 ng/m L 7/22/201 1 4:41:4 9 A

M Ye s Specif y Paramet e 5 0 Base . Sub . Window : 1.0 0 ml n Peak-Split . Fact e Repor t Larges t

Pe Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Area : 7 2 Height : Star t Time : En dTime : r : 3 a k : Ye s 0.0 0 cp s 0.0 0 se c 30. 0 ae c 13. 2 mi n N o Manua l 13. 2 ml n 169 4 count s .lOetOO S cp s 12. 9 ml n 13. 5 ml n 1.05e 5 1.00e 5 9.50e 4 9.00a4 ' 8.50* 4 8.00e 4 7.50* 4 7.00e4 : 6.50*4
- § . 6.00*4 '
^ 5.50* 4 I5.00*4 450e4 ' 4.00* 4 3.50* 4 3.00*4
- 2.50* 4 2.00* 4 1.50*4 ' 1.00e 4 5000.00
- 0.0 0 12. 0 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 f^ile : i d 1072ba.wi r .00 0 Da llfle . N o Proc , Algorithm : Specif y Parameter s - Nots ePercentage : 5 0 Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s ght : Min . Pea k

Width : Smoothin g Widt l R TWindow : Expecte dRT : 0.0 0
! Rela i : RT : N o Int . Type : Valle y Retentio nTime : 13. 1 ml n Area : 32158 0 count s Height : 5.07e-f00 4 cp s Star t Time : 12. 8 ml n En dTime : 13. 3 ml n 

# I SarT4]4eNam*:'V110720a13 rSampl e ID : *GLP1(K)1-02- 1 Pea k Name : -PFBS ' Masa(*s) : -299.00(V80.00 0 Da,299.000/99
. Co[iwnent'OALGW134S011062 rAnnexation:" ' Sampl eType : Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Bas« . Sub . Klnd o Peak-Split . Fac t Unknow n N/ A 0.25 5 7/22/201 1 9:14:2 5 A

M Ye s Specif y P a 5 0 * : 1.0 0 r : 4 Repor t Larges t

Peak : Ye s Mln , Pea k

Heigh t Min , Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Retentio nTime : 0.0 0 0.0 0 30. 0 a 13- 2 B No Valle y 13. 2 « Area : 35839 8 cou r Height : 5.S4e+00 4 e Star t Time : En dTime : 12. 9 n 13. 5 n

12. 5 13. 0 13. 5 5.4e4
- 5.2e 4 5.0e 4 4.8* 4 4.6*4
- 4.4*4
- 4.2*4
- 4.0*4
- 3.8e4
- 3.6*4
- 3.4*4
- 3.2*4
- 3.0*4
- ' 2.8*4
- Z6e4
- 2.4*4
- 2.2e4
- 2.0*4
- 1.8*4
- 1.6*4
- 1.4*4
- 1.2*4
- 1.0*4
- S000.0
- 6000.0
- 4000.0
- 2000.0
- 0.0
- 12. 0 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e5 o f 2 9 

### Pag e 5 8 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b

I I I Sampl * Name:-k110720a15 rSampl e b:-QLP1«>t-02-1&«69 - Flle:1c11D720a.M r I P*a k

Nam* : -PFBS " Hae»(»«): •299.O0Om0.DO0 Da,299.000^9.00 0 Da - •Comment : -DA LG W13S S 0110628 " Annotation : ~ Sampl e Index : 15 2 Sampl e Type : Unknow n Concentration : N/ R Calculate d Cone : 1.2 0 ng/m L,4* 5 Acq . Date : 7/22/201 1 Acq . Time : 2:08:0 7 P M 2.3e 5 Modified : ye s 2Je 5 Proc . Algorithm : Specif yParameter s - M O II I Nois e Percentage : 5 0 ^•^' ^ Base . Sub
. Window : 1.0 0 mi n 2.Qa S Peak-Split
. Factor : 4 Repor t Larges t Peak: - Ye s 1,9« 5 Min . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se cI.Se S Smoothin g Width : 0 point s R TWindow : 30. 0 se c'-'• ' Expecte d RT : 13. 2 ml n . g^ c Us e Relativ e RT : N o 1.5e S Int . Type : Bas e T o Bas e Retentio n Time : 13. 1 ml n « 1.4* 5 Area : 166629 2 count s g " ., , Height : 2.50 e + 00 5 cp s < . •••* " Star t Time : 12. 9 ml n | ,^ ^ En d Time : 13. 7 ml n fe £1.1* 5 1.0* 5 9.0* 4 8.0* 4 7.0* 4 6.0e 4 5.0e 4 4.0e 4 3.0* 4 2.0* 4 1.0* 4 11. 0 11. 5 12. 0 I Sampl*N8m«:T(110720a166 - Sampl e ID : •GLP1{M)l-02-1W)79 - File:T(110720a.wH r I Comment:"DALGW135R0110628(dai:100) * Annotatton: " Sampl e Index : 16 6 1 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 74. 9 ng/m L1 Ja S Acq . Date : 7/22/201 1 Acq . Time : 7:01:5 6 PM 1.6* 5 Modified : Ye s Proc . Algorithm : Specif yParameter s - M Q II I .c- c Nois e Percentage : S O '^ ^ Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 3 1.4* 5 Repor t Larges t Peak : Ye s Kin . Pea k

Height : 0.0 0 cp s ^ 3t S Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s R TWindow : 30. 0 se c1-2« 5 Expecte d RT : 13. 2 ml n Us e Relativ e RT : N o 1_,, 5 Int . Type : Bas e T o Bas e Retentio n Time : 13. 2 ml n t , ••0« 5 Area : 104195 7 count s fr Height : 1.76et00 5 cp s ^ 9.0* 4 Star t Time : 12. 8 mi n H
7.0* 4 6.0* 4 5.0e 4 4.0* 4 3.0* 4 Z0* 4 1.0* 4 11. 0 11. 5 12. 0 1 1 .1 4

1 

# 1 • 

# 1 1 

# X
12 5 13. 0 13. 5 14. 0 14. S

T^m* . mi n

131 8



# I
12. 5 13. 0 13. 5 14. 0 14. 5

Time , ml n 1 1 Sampl e Name : •k110720a15 rSampl e ID:-GLP1W)1-02-1WJ74 - Fae:-k11O720a.wt r 1 •Pea k

Name : -PFBS ' Ma3a(**) : -299.00a«0i)0 0 Da.299.00CV99.00 0 Da 15. 0 15. 5

15. 0 15. 5 •CommcntT)AlGW135L0110628<dl1:10) - Annotatton: " Sampl e Index : 15 7 Sampl e Type : Unknow n Concentration : N/A Calculate d Cone : 9.7 3 ng/m L Acq . Date : 7/22/201 1 2.2e 5 Acq . Time : 3:53:0 3 P M Modified : N o ,(
. - Proc . Algorithm : Specif yParameter s - M Q II I ^"' ^ Nois e Percentage : 5 0 . g^ c Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 2 ig^ g Repor t Larges t Peak : Ye s Min . Pea k

Height : 0.0 0 cp s u^ S Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 1.6e 5 R TWindow : 30. 0 se e Expecte d RT : 13. 2 ml n 1.5* 5 Us e Relativ e RT : N o 1.4* 5 Int . Type : Valle y Retentio n Tlms : 13. 2 ml n a '•3e 5 Area : 135231 3 count s 9 . - - Height : 2.3OetO0 5 cp s > l '-^ ^ Star t Time : 12. 9 ml n 1 i i «: En d Time : 13. 5 mi n •'''" ^

- 1.0* 5 9.0e 4 8.0* 4 7.0* 4 6.0* 4 S.0e 4 4.0* 4 3.0e 4 2.0* 4 1.0* 4 11. 0 11. 5 ii o 1 Sample^3me:-k110720al V Sampl e ID:-GLP1&O1-02-1M>8 rt'ile:-k110720a.wi r I Pea k

Name:-PFBS - MaB9(ea):-299.000>f80.00 0 Da,299.D00i^.OO O Da ' •Comment'DALGW136S0110630{dai:10)' ' Annotation: ' Sampl e Index : 17 9 Sampl e Type : Unknow n Concentration : M/ A Calculate d Cone : 3.7 1 na/m L Acq . Dale : 7/22/201 1 8.0B 4 Acq . Time : 11:35:0 1 P M 7.5* 4 Modified : Ye a Proc . Algorithm : Specif yParameter s - M Q II I Nois e Percentage : 5 0 7.0* 4 Base . Sub
. Window : 1.0 0 ml n Peak-Split
. Factor : 2 Repor t Larges t Peak : Ve s ''•'* ^ Min . Pea k

Height : 0,0 0 cp s Min . Pea k

Width : 0,0 0 se c6.0e 4 Smoothin g Width : 0 point s R TWindow : 30, 0 se c Expecte d RT : 13. 2 mi n 5.5* 4 Us e Relativ e RT : N o 5.0* 4 Int . Type : Manua l Retentio n Time : 13. 2 ml n « Area : 51891 6 count s fr4.5* 4 Height : 8.39e+00 4 cp s > ; Star t Time : 12. 9 ml n 1 En d Time : 13. 4 ml n g *-'* ^ 3.5* 4 3.0* 4 Z5e 4 2.0e 4 1.5e 4 1.0* 4 5000. 0 11, 0 11. 5 12 0 13.2 2 1



## f 12. 5 13. 0

Tim e 

### i .

13. 5 14. 0 14. 5 15. 0 15. 5 •m h

131 7 1

•,. , ,-.-,,- A 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e6 o f 2 9 

### Pag e 5 9 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

i*:"k110720a170 - Sampl e ID : •GLP1(W)1-02-18O84 " Flle:"k110720a.wi r Pea k Name : -PFBS ' Maaa<es> : -299.000/80.00 0 Da,299.000/99,00 0 Da Comm*nt:-DALGW136L 0 11063 0 (dl l 1:10) " Annotatton: - Sampl e Index : 17 0
5,2* 4 5,0* 4 4.8* 4 4.6* 4 4.4* 4 4.2* 4 4.0*4 ' 3.8e 4 3.6* 4 3.4*4
- 3.2*4
- 3.0*4
- 2^*4
- 2.6*4
- 2.4*4
- 2.2*4
-

1.4* 4 1.2* 4 1.0* 4 8000. 0 6000. 0 4000. 0 2000. 0 Sampl eType : Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc , Algorithm : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Unknow n N/ A 2.3 3 7/22/201 1 B:25:5 7 P

M No Specif y P a : 5 0 w : 1.0 0 o r : 2 Isepor t Larges t

Peak : Ye s Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : : 0.0 0 0.0 0 0 30. 0 13. 2 n N o Valle y 13. 2 n Area : 32799 6 cou r Height : Star t Time : En dTime : 5.61e+00 4 c 12. 9 n 13. 4 n

0. 0 11. 0 13. 0 13. 5 

# I Sampl*Nam*:'K110720a19 rSampl e ID:-GLPlOo4-02-18^ - ^lie:-k11O720a.wi r Pea k Name : -PFBS " MaBs(e3) : -299,000^0.00 0 Da,299.000/99.00 0 Da " Comment:-DA LGW137 L0110624 - Annotatton : " Sampl eIndex : 19 3 Sampl eType : Sampl * Nam* : 1(110720a184 - Sampi s ID:-GLP1tHll.02-18-09 rFlle:-k110720a.vri r P*a k Name : 'PFBS - Ma9S<es) : -299.000/80.00 0 Da.299.000/99.00 0 Da Commen t "DA LGW137S0110624 - Annotation; - Sampl e Index : 18 4 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 0.10 8 ng/m L Acq . Date : 7/23/201 1 Acq . Tims : 1:20:0 3 A M Modified : Proc . Algorithm : Nois e Percentag e Base . Sub , Klndo u Peak-Split
. Fact o Repor tLarges tP

e Min . Pea k

Heigh t Mln . Pea h Width : Smoothin g Width : R TWindow : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : N o Specif yParamet e 5 0 : 1,0 0 mi n r : ? 0-0 0 cp s 0.0 0 se c 0 p o 30. 0 se c 13. 2 ml n N o Bas e T o Bas e 13. 2 ml n Area : 15392 2 count s Height : 2 .42et0O 4 cp s 2.4* 4 Z3* 4 Z2o 4 2. 1 e 4 2.0e 4 1.9e 4 1.8e 4 1.7e 4 1.6e 4 1.Se 4 1.4* 4 1 1.3e 4 § •1.2e 4 1 1.1* 4 1.0* 4 9000. 0 80DO. O 70O0. 0 6000. 0 5000. 0 4000. 0 3000. 0 2000. 0 1000. 0 0. 0 11. 0 14S 2 WN»^iM''Wll*<*!M> M 12. 5 13. 0 13 5 14. 0 14. 5 IS. O 15. 5 

# I SampleName:*1(110720s196 - Sampl e ID:-GLP1CM)l-02-18-10 rFlle:-kll0720a.wi r Pea k Name : -PFBS " Maaa(ea) : -299.000re0,00 0 Da.299.00CV99.00 0 Da * Comm*nt-DALGW138R0110630(d01:10) - Annotation:'* - Sampl eIndex : 19 B Sampl eType : Calc i N / A nq/m L 2.0* 4
Modified : No Proc . Algorithm : Specif y P a Nois ePercentage ; Base . Sub . Window : Peak-Split . Facto r Repor t Larges t

Pe a Hln . Pea k

Height : Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : B Retentio nTime : 5 0 1.0 0 2 : Ye s 0.0 0 0.0 0 0 30. 0 13. 2 N o s eT o B a 13. 2 Area : 11955 1 co u Height : 2.03e+00 4 Calculate d Cone : Acq . DatQ : Acq , Tlmfl : N / A

Nols i Per t itag e Bas i Peak-Split
. Factor : 4 Repor tLarges t Peak : Mln . Pea k

Height : ( Mln . Pea k

Width : ( Smoothin g Width : ( R TWindow : 30. 0

Specif yParamete i 5 0 • : 1.0 0 mi n

.ela ' ! RT : Bas e T o Bas e

4 6 8 9 count s Height : Star tTime : En d Time :

14. 0 14. 5 15. 0 15. 5 . Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , Pag e7 o f 2 9 201 1 

### Pag e 6 0 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSarriple s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b 

# I Ssmpl*Nam*:-k110720a207 ' Sampl e ID:-GLPiaO1-O2-18-106
- Flle:1(110720a.w() r Pea k Name : 'PFBS
- Ma*8<*8} : -299.000/60.00 0 Da,299.000il99,00 0 Da
Comment:-OALGW138L0110630 r Annotatton: " Sampl e Index : 20 7 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : 0.038 5 ng/m L Acq . Date : 7/23/201 1 Acq . Time : 9:22:5 4 A M Ho d i f Proc . Nois e Base . ^eak Repo r Mln . Min . ird :

A l g P e r Sii h Spl l : L a 'ea k 'eal r Smoothin g R TW i Expe e Us e R Int . Rete n Area : Heig h Star t t e d ela t Typ e I o n 3rith m :entag . Wind o

. Fac t •ges t 1 Heigh i Widt h Widt h T : v e R T Time : Time : En d Time : N o Specif y : 5 0 P a r w : 1.0 0 o r : 2 eak : Y. : 0

0 0 30. 0 13. 2 N o Bas e T o 13. 2 5727 7 c 9.49e+00 , 13. 1 13. 4 O U

(K l

m n Bas e m o u n s c p s m n



# I San^Name : 1(1107203221
- Sampl e ID:'GLPiaO1-02^1S-1i r Flle:1(110720a.w(r r Pea k Name : -PFB r

Masa(*s) : *299.000/80.00 0 Da,299.000/99.00 0 Da
Comment : "DA

LGWCW26C0110622(ci a1:10) * Annotatk>n: ~ Sampl e Index : 22 1 3.2*4 - 3,0*4 -

25* 4 2.0* 4 1A*4
- 1.6*4 ' 14. 0 14. 5 15. 0 15. 5 

# I Sampl * Name:-k11D720a2i r Sampl e lD:-GLP1W)l.02-18-1i r Fl»e:-k110720a.wi r Pea k Name : -PFBS
- Mass<es) : -299.000/80.00 0 Da,299.000/99.00 0 Da
Commen t

"DALG W138S 0 110630(d a1:10)
- Annotatlon:'
- Sampl e Index : 21 2 Sampl e Type ; Unknow n

Nol l :ag e 5 0 r : 1.0 0 Base . Sub . windo w Peak-Split . Factor : 4 Repor t Larges t

Peak : Ye s Min . Pea k

Height : 0.0 0 Min . Pea k

Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30, 0 s f i Rel i Rctent l Height : • RT : Bas e T o Bas e

• : 13. 2 mi n $3558 3 count s 9,44e+00 4 cp s 12, 9 mi n 13, 5 mi n 9,0e4
- 8.5*4
- 8.0e4 ' 7.5*4
- 7.0*4
- 6.5*4
- 6.0*4
- 5.5*4
- 5.0e4
- 4.5*4
- 4.0e4
- 3.Se4 : 3.0e 4 2.Se 4 2.0e 4 1.5*4 ; 1.0*4
- 5000.0
- 0.0 - 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 

# I Sampl e Name : 1(110720a226 : Sampl e 1D:*GLP1U)1-02-18-123
- File : Pea k Nam* : 'PFBS
- MB>a(es) : -299.000/80XW O Da39.00a^.00 0 Da
Comment-DA LeWCW26L0110622(d U1:10) " Annotatton:
- Sam p Con e . e Typ e entrati c U

n Calculate dCone : Acq . Acq . Mod i Pro c Noi s Bas e Pea k Rep o Min . Min . Date : Time : fled : Algo r e Perce i 7 /

2 : thm : S p Sub . Window : -Split . r t Larg e Pea k H e Factor : S t Pea k ight : Pea k width : Smoothin g width : R TK E^ip e Us e Int . Ret e Are a ndow : =te dR T Relativ e Type : itlo nT Height : Sta r Time : RT : M

233 3 3 . 3 kn o w n N/ A 2 3 / l b : Y
2 6 201 1 5 2 P M s eclf y F a

3 0 . 1 :
1 1 l e t y ^ 5 0 1.0 0 Ye s 0.0 0 0.0 0 0 D a

a l

, 2 1 )0 4 C

, 9 n ampl e Type :

8000. 0 6000. 0 4000. 0 2000.0
- N/ A

12. 0 12. 5 13. 0 13. 5 Calculate dCon e Acq . Date : Acq . Time : Modified : Proc . Algor i Nois e Pecce n Base . Sub . K Peak-Split . Repor t Larg e Mln . Pea k

He h m 13. 4 7/23/201 1 4:01:3 9 P

M Ye s Specif y P a age : 5 0 ndow : 1.0 0 •a c t Peak : Ye s (T h Kin , Pea k

Widt h Smoothin g w l R TWindow : Expecte dRT : Us e Relativ e Int . Type : Retentio nT l Area : Height : Star t Time : R

T : 0.0 0

0.0 0 0 30, 0 3 13, 2 n No Valle y 13. 1 p 3 7 665 0 eou n 5,28e+00 4 c 12, 9 n

^J..)!..' , fit: , i »,l4«miM i 14. 0 14. 5 15. 0 15. 5 12. 5 13. 0 13. 5 14. 0 14. 5 15. 0 15. 5 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e8 o f 2 9 

### Pag e6 1 O f 11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b

||Sampl*Name:-K110720a237 - Sampl e ID:-GLP1(M1-02-18-128 - FlU:^110720a,wi r I Pea k Name : "PFBS " Maes(*s) : "299,000«),00 0 Da,299.000«9,00 0 Da " •Comment:-DALGW140R0110e22(dm:10) - Annotatton: - Sampl e Index : 23 7 Sampl e Type : Unknow n Concentration : N/A Calculate d Cone : 1.5 8 ng/m L Acq . Date : 7/23/201 1 3,0* 4 Acq . Time : 7:52:1 6 P M Modified : Ye s 2.8* 4 Proc . Algorithm : Specif yParameter s - M Q l U Nois e Percentage : 5 0 oc. ^ Base . Sub
. Window : 1,0 0 mi n ^'" ^ Peak~Spllt , Factor : 2 Repor t Larges t Peak : Ye s 2.4* 4 Min . Pea k

Height : 0.0 0 cp s Min , Pea k

Width : 0,0 0 se c Smoothin g Width : 0 point s 2.2* 4 R TWindow : 30, 0 se c Expecte d RT : 13, 2 ml n Us e Relativ e RT : N o 2.0* 4 Int . Type : Manua l Retentio n Time : 13. 2 ml n . '••' ^ Area : 22258 2 count s g " Height : 3,16 e + 004 cp s < . ,- ^ Star t Time : " 12. 9 mi n % En d Time : 13. 4 mi n | •£1,4* 4 1,2* 4 1,0* 4 8000. 0 6000. 0 4000. 0 2000, 0 j m ^

11. 0 11. 5 12. 0 12 5 1 SamptefJame:^110720a25 rSampl e lb:'•GLP1(W1-02-18-140 ^ l=lle:i(11D720a.** r I Pea k Nam* : -PFBS - Mas3(e3) : -299.000/80.00 0 Da.299.000/99,00 0 Da " •Commen(:'DALGW142R0110622(dil1:10) - Annotation:" " Sampl e Index : 25 1 Sampl e Type : Unknow n 1 Concentration : N/ A3.4* 4 Calculate d Cone : 1.6 9 ng/m L Acq . Date : 7/24/201 1 - - . Acq , Time : 12:46:2 9 A M ^"^' ^ Modified : N o 3,0*4 ' Proc . Algorithm : Specif yParameter s - M Q II I 1 Nola e Percentage : 5 0 Base . Sub
. Window : 1,0 0 ml n ^^*^ ' Peak-Split
. Factor : 2 Repor t Larges t Peak : Ye s 2.6*4 - Min . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 2.4*4 - R TWindow : 30. 0 se e Expecte d RT : 13. 2 ml n 22*4 - Us e Relativ e RT : N o Int . Type : Valle y2,0*4 - Retentlo n Time : 13. 2 ml n - Area : 23635 1 count s § , g^ . Height : 3.426^^00 4 cp s ^ Star t Time : 12. 9 ml n B En d Time : 13. 4 ml n | 1.6*4 - 1,4*4 ' 1.2*4 ' 1J)e4 - 60O0.O eooo . • 4000 . - 2000 . - 11. 0 11 5 12. 0 12. 5 1 13-1 7



## ^ 13, 0 13, 5 14, 0 14 5 15. 0 15 5

13,1 6

• A 13. 0 

# 1

13 5 14. 0 14 5 15. 0 15 5 1 Pea k Name : 'PFBS ' Mas*{*s) : -299,000/80.00 0 Da,299,000/99.00 0 Da ' ' •Comment-OALGWUlROIIoeSO " Annotation:- " Sampl e Index : 24 6 ; Sampl e Type : Unknow n 1 Concentration : N/ A Calculate d Cone : 0,043 7 ng/m L950 0 Acq . Date : 7/23/201 1 Acq , Time : 11:01:1 7 P M goo Q Modified : N o -- - Proc . Algorithm : Specif yParameter s - M Q II I "^^ ^ Nois e Percentage : 5 0 Base , sub . Window : 1.0 0 mi n 800 0 Peak-Split
. Factor : 2 Repor t Larges t Peak : Ye a 750 0 Min . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se c70O O Smoothin g Width : 0 point s R TWindow : 30, 0 se c^CO Q Expecte d RT : 13. 2 ml n Us e Relativ e RT : N o ^ ^ Int , Type : Bas e T o Bas e Retentio n Time : 13, 2 mi n « 550 0 Area : 6439 5 count s & Height : 9.e0e+00 3 cp s ^ 500 0 Star t Time : 13. 0 mi n • » En d Time : 13. 4 mi n •450 Q 400 0 350 0 300 0 250 0 200 0 150 0 100 0 SO O 10,8 5 131 9 1

12.96, . 11. 0 11. 5 12 0 12. 5 13, 0 1 Sampl e Name : •K110720a2eo - Sampl e ID : "GLPI&OI^2-18-145 " File:Sdl0720a.wi r I Pea k Nam* : "PFBS " MaBS(ea) : •299,000i«).00 0 Da.299,000«9,00 0 Da " •Comment-DALGW143R0110622{dai:10) - Annotation: - Sampl e Index : 26 0 Sampl e Type : Unknow n Concentration : N/A Calculate d Cone : 1.6 4 ng/m L Acq . Date : 7/24/201 1 3-*^ ' Acq , Time : 3:55:3 9 AM 3.20 4 Modified : Ye a Proc . Algorithm : Specif yParameter s - M Q II I Nois e Percentage : 5 0 ^-°'* ' Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 3 Z8*4 - Repor t Larges t Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s _„, . Min . Pea k

Width : 0.0 0 ae c^'^ ^ Smoothin g Width : 0 point s R TWindow : 30. 0 ae c2.4*4 - Expecte d RT : 13. 2 ml n Us e Relativ e RT : N o 2,2*4 - Int , Type : Bas e T o Bas e Retentio n Time : 13. 2 ml n « 2.0*4 - Area : 23148 0 count s g " Height : 3,55e*00 4 Cps > ; , n^ . Star t Time : 12. 9 mi n % En d Time : 13- 4 ml n 0 c1.6e4 ' 1.4*4 - 1,2*4 - 1.0*4 - 8000,0 - 6000.0 - 4000.0 - 2000.0 - Tim * i

### i

1 13.9 1 14,59, . 14,8015.0 7 \ ^ ^^13. 5 14,0 ^ l4. S lt! o 15. 5 .ml n

131 AI I

11. 0 11 5 12, 0 12, 5 13. 0



## •"" ^ 13 5 14. 0 14 5 15. 0 15. 5 i^ 5 —^ ^ = U Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e o f 2 9 

### Pag e 6 2 of11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS
. PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b 

# I SampleName:"k110720a265 - Sampl * ID : "GLP1(M1.02-18-150 " FU* : P*a k Nam* : 'PFBS - Man<*9) : -299,000/80.00 0 Da.299.000/99.00 0 Da Comment : "DA LGW144S 0 11062S(dl l 1:10) - Annotatton:" " Sampl eIndex : 26 5 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 1.6 9 ng/m L Acq . Date : 7/24/201 1 Acq . Time : 40:4 3 A M Modified : Pro c Nois e Base . Peak Min . Mln . Smoo t R TW J Expe c Us e R Int . Rete n Area : Heig h Star t En d T Y e s Algorithm : Specif yP a P e r S u b Spl l : L a F-ea k Sfla k iln q Le d ela t Typ e 

## : : T i m Ime : centaqe : Window :

. Facto r rges tPe a Height : Width : Width : ! T : v e RT : B Time : 2 3 8 S O

1.0 0 3 0.0 0 0.0 0 0 30. 0 13. 2 N o s e T o B a 13. 2 n 3 6 co u 3.78et00 4 12. 9 13. 5 B 3.6* 4 3,4* 4 3,2* 4 3.0* 4 za* 4 Z6* 4 2.4* 4 2.2* 4 2J3a 4 15* 4 1.6* 4 1.4* 4 1.2* 4 1.0* 4 8000. 0

4000. 0 2000. 0 0. 0 ..ni l ^iHii^.l . ti(W^i4ii.wV*>M,n(*e*^*^r t 12. 5 13. 0 13 5 14. 0 14 5 15. 0 15 5 

# I Sampl * Nam* : •kl10720a145 " Sampl a ID : •GLP1&O1.02-18O6 F Fil* : "k l 10720a.wM r P*B k Nam* : "PFBS " Maaa<*s) : "299.000/80.00 0 D8,299.000i99.000 Da Commen t Tri p Blan k

Set1 MS ' Annotation:" " Sampl eIndex : 14 5 Sampl eType : Unknow n

tlO i N/A Calculate dCon e Acq . Time : Modified : Proc . Algorith m 10. 3 ng/m L 7/22/201 1 11:41:1 5 A

M Ye s Specif y Parameter s Nois ePercentage : 5 0 Base . Sub . Wind c Peak-Split . Fa c Repor t Larges t Min . Pea k

Heig h Min . Pea k

Widt h Smoothin g Widt h R TWindow : Expecte dRT : Us eRelativ eR

T Int . Type : Retentio nTime : w : 1.0 0 ml n

o r : 2 eak : ye s : 0.0 0 cp s

0.0 0 se c 0 poin t 30. 0 se c 13. 2 ml n N o 13. 1 rain Area ; 1392115 8 count s Height : Star t Time : En dTime : 2.15e*00 6 cp s 13. 0 ml n 13. 6 Bi n - M QII I



## 1 1 2. 1 e 6
1.9* 8
1.7* 6
1.5* 6 1.4* 6 1.3e 5 1,2e 6 1.1* 6 1.0* 6 9.0* 5 8.0*5
- 7.0*5
- 6,0e S 5,0* 5 4,0* 5 3.0* 5 2,0* 5 1.0e S UJ Q 13. 0 13 5 14. 0 14. 5 15. 0 15 5 

# I Sampl e Name : 1(110720a018 - Sampl e ID:-11003.2fr9 - Fle:-kll0720a.wi r Pea k Name : -PFHS * Msss(es) : -399.000/99.00 0 Da,399.000nK).00 0 Da Comment ; "2 5 nflftn LF C rid h M O Q Water " AnnoUtton: - Sampl eIndex : 1 8 Sampl eType : Concen t rati o Calculate dCon e Acq . Date : Acq . Time : Modified : Proc . Algor i Nola ePerce r Base . Sub . V Peak-Split . Repor t Larg e Min . Pea k

H e Mln . Pea k

W i Smoothin g W

i R TWindow : Expecte dRT : Us eRelativ e Int . Type : Retentio nT l Area : Height : Star t Time : En dTime : .h m

.n d Standar d

2.5 0 ng/m L 2.6 2 ng/m L 7/20/201 1 3:17:0 5 P

M No Specif y Paramet e : 5 0 w : 1.0 0 ml n "actor : 2 S t • q h fit h d t h R T eak : Ye s : 0.0 0 cp s

0,0 0 se c 0 p o 30. 0 se c 14, 9 mi n N o valle y 14, 8 mi n 2010 5 counts : 6.19e-f00 5 cp s 14. 7 ml n 15. 2 ml n 

# I Sampl e Name : 1(110720a6S ^ Sampl e lo;'SolveniBlank - t^ite:'Tc110720a.*lf r Pea k Name : 'PFHS " Masates) : -399.000/99,00 0 Da.399,000/80,00 0 Da Commen t -M m Q V\Me rTN I 1-0238 " Annotatton;" "

Unk i Sanpl eType : Concentration : Calculate dCone : Acq . Date : - 7/ : Acq , Time ; 6: . Modified : Proc , Algorithm : Sp i Base , Sub . Window : Peak-Split , Factor : Repor t Larges t N/A

Smoothin g i R TWindow : Expecte dR

^

Area : Height : Star t Time : En dTime :

:a k Height : c p s

16 5 17. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 0 o f 2 9 

### Pag e 6 3 of11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

•SampleNamB:-k110720a029 " Sampl e ID:'LCS-110714-1 " Fle;"k110720a.wi r I Pea k Name : "PFHS " Mas9{ea) : "399.000*9.00 0 Da.399.000«).00 0 Da " I Comment : '05pt> b LCS " Annotation: " Sampl e Index : 2 9 Sampl e Type : Q C Concentration : 0,49 8 ng/m L Calculate dCone : 0.52 4 ng/m L Acq , Date : 7/20/201 1 Acq . Time : 7:07:2 1 P M Modified : Ye s Proc . Algorithm : Specif y Parameter s - M Q II I Base . Sub . Window : 1,0 0 ml n Peak-Split , Factor : 2 Repor t Larges t

Peak : Ye s Mln , Pea k

Height : 0,0 0 cp s Mln , Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s R TWindow : 30, 0 se e Expecte dRT : 14. 9 ml n Us e Relativ e RT : No Int , Type : Manua l Retentio nTime : 14, 8 ml n Area : 94.177 5 count s Height : 1.24et00 5 cp s Star t Time : 14. 7 ml n 

## 1 1.20e 5 1.15e 5 1.106 5 1.05e 5
9.50e4 ^
8.50e 4 8,00e 4 7.50* 4 7.00* 4 6.50* 4
5.50* 4 5.00* 4 4.50* 4 4,00* 4 3.50* 4 3.00*4 ' 2,50*4
- 2.00* 4 1.50* 4 1,00* 4 sooao o 0.00 ' 

# I Sampl * Name:'\11D720a03S - Sampl e 10 : *LC&-110714-7- Fl*:'k110720a.wif r Pea k Name : 'PFHS ' Mas9(es> : -399.000/99.00 0 Da.399.000/80.00 0 Da ' Conunent : -30pp b LCS - Annotation: " Sampl e Index : 3 5 Sampl e Type : Q C Concentration : 29. 9 ng/m L Calculate dCone : 30. 1 nq/m L Acq . Date : 7/20/201 1 I Sampl e Name : 1(110720a032 - SamplelD:TCS-110714-4 ' Fie : "k110720a,wtf r IPea k Name : • Commen

t "Spp b LCS " Annotatton : - Sampl e Inde : Sampl e Typ e Concentrati o Calculate di Acq , Date : Acq , Time : Modified : Proc . Algor : Nois e Perce r Base . Sub , 1 Peak-Split . 3 2 O C n : 4.9 8 one : 5.2 1 7/20/201 1 8:10:1 2 P K o ng/m L

ng/m L

thm : Specif y Parameter s tage : 5 0 indow : 1.0 0 Factor : 2 S t Peak : Ye s Min . Pea k

Height : 0. 0 Min . Pea k

W l Smoothin g W

: R TWindow : Us e Relativ e Int , Type : Area : - Height : Star t Time : En dTime : dth : 0, 0 30. 0 RT : N o Valle y 810734 2 C

O ].19et00 6 14. 7 15. 4 c p s

s e c

n t s cp s ml n ml n - M

Q 11 1



# a t 1.15* 6 I.IOeO
1.05* 6 1.00* 6
9.00e 5

7.50* 5 7.006 5

6.00B 5 5.50* 5 5.00* 5 450* 5 4.00* 5 3506 5 3.006 5 2.506 5 2.00e 5 1.506 5 I.OOa S 5.006 4 0.0 0 12. 5 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 

# I Sampl e Name:-V110720a042 - Sampl e ID:'LCS-110714-10 " Fl)e:-k110720a.w« r Pea k Name : "PFHS " Ma«e(ea} : -3g9.00(V99.00 0 Da,399,000/80.00 0 Da Commen t -O.Spp b LC S ECP - Annotation:" " Sampl e Index : 4 2 Sampl e Type : ("C Concentration : 0.0 0 ng/m L6500 - Calculate d Cone : 0.024 5 no/m L Acq . Date : 7/20/201 1 55e 6 Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e Base . Sub . Wind o 9:13:0 S P M N o Specif y P a 5 0 J : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : 0.0 0 0.0 0 0 30- 0 14. 9 n No Valle y 14. 8 Area : 4179996 9 C O Height : 5.74e-t00 6 Star t Time : En dTime : 15. 2 I t Acq . Tim e Modified : Proc . Algorith m Nois e Pe r Base . Su b 11:39:5 3 P M N o Spe c Window : Peak-Split . Fac i Repor t L a Hln . Pea k Min . Pea k Smoothin g R TWindo w ges t

1 Helgh i Widt h Widt h Expecte dRT : Us e Rela t Int . Typ e Retentio n Area : Height : v e R

T Tlmft : eak :

3 0 1 t v S O Param e 1.0 0 m l 7 Y 0 0 0 0

. 9 N o Bas e 5499 2 T o s

0 0 c 0 0 3 s e c ml n Bas e ml n 6.49e-f00 3 cp s

Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 1 o f 2 9 

### Pag e 6 4 O f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

|l|5.mpl.Nam*:-K110720a045
- Sampl * ID:-LC5-110714-i r Flle:-k110720a,vri r IPea k Nama : "PFHS " Ma3S<*a) : •399,000»9.00 0 D».399.000ra0,00 0 Da * • Comm*nt : "Spp b LC S ECF " Annotatton : " Sampl eIndex : 4 5 Sampl eType : Q C ,48 2 Concentration : 0.0 0 ng/m L5/4* 4 Calculate dCone : 0.25 7 ng/m L Acq , Date : 7/21/201 1 '-^ M Acq . Time : 12:42:4 9 A M 50, 4 Modified : Ye s 4,8* 4 Proc . Algorithm : Specif y Parameter s - M Q II I4- ^ Nois ePercentage : 5 0 '-^" ^ Base . Sub . Window : 1,0 0 ml n4.4* 4 Peak-Split . Factor : 4 Repor t Larges t

Peak : Ye s 'tuv t Mln . Pea k

Height : 0.0 0 cp s ' 40 ^ Mln . Pea k

Width : 0.0 0 se e Smoothin g Width : 0 point s 3,8* 4 R TWindow : 30. 0 se c ,- . Expecte dRT : 14. 9 ml n•^•'** * Us eRelativ eRT : No 3.4 M Int . Type : Bas eT o Bas e3.2* 4 Retentio nTime : 14. B rain « 30 ^ Area:
- 42391 S count s & Height : &.47et00 4 cp s ^ 2.8* 4 Star t Time : 14. 5 mi nV En dTime : 15, 2 Bi nS • EZ4* 4 25* 4 2.0* 4 1.8* 4 1.6* 4 1.4* 4 1.2* 4 1.0* 4 8000. 0 6000. 0 4000. 0 2000, 0 14.7 5 I I 

# u 

# n 1

. J

' li s 13. 0 13 5 14, 0 14 5 

# 1
15. 0 15 5 16. 0 16 5 17. 0 1 Tim eml n • Sampl eName:-kll07203061 " Sampl eID:-GLP1W)1-02-18.00e " FUe:1(110720a.w<i r 1 Pea k Nam* : -PFHS " M«ss(e8) : -399,000/99.00 0 Da,399.000/80.00 0 Da
- • Comment : "DALG W130 R011063 0 (dl l 1:100)
- Annotatton: " Sampl eIndex : 6 1 Sampl eType : Unknow n148 3 Concentration : N/ A Calculate dCone : 125 . ng/m L^.66 5 Acq . Date : 7/21/201 1 ,56 5 Acq . Time : 6:18:0 6 A M 2.46 5 Modified : Ve s -i-i-i : Proc . Algorithm : Specif y Parameter s - M Q II I^.36 5 Nois ePercentage : 5 0 2.2* 5 Base . Sub . Window : 1.0 0 mi n Peak-Split . Factor : 3 2.1* 5 Repor t Larges t

Peak : Ye s •>«-• ; Min . Pea k

Height : 0.0 0 cp s ^" ^ Min . Pea k

Width : 0.0 0 se c I.90 5 Smoothin g Width : 0 point s R TWindow : 30. 0 se c 156 5 Expecte dRT : 14, 9 ml n, ,. - Us eRelativ eRT : W o '''* * 1.66 5 Int . Type : Manua l Eetentior , Time : 14. 8 mi n« '-^ ^ Area : 199058 0 count s g - 14 ^ Height : 2.65e-f00 5 cp s > L Star t Time : 14. 6 mi n' S 1.3e 5 UndTi.. , ,S. l .i n1 _^ ^ 1.16 5 1,06 5 9,0e 4 8.0e 4 7,0e 4 6,0e4
- 5,0*4
- 4,0* 4 3.0e4 ' 2.06 4 1.0* 4 14.69, , 1 

# 1 12. 5 13. 0 13 5 14. 0 14. 5 

## =

____ _• 

## - ^ 

# 1



# I
15. 0 15 5 16. 0 16 5 17. 0 |isampt*N»m*:T.110720a048 " Sampf eto:-LCS-110714-16 " FBe : "k110720a.v»! r IPea k Nam* : 'PFHS " Ma3S<*a} : -399.000/99.00 0 Da.399.000/80.00 0 Da
- • Conunen t

-30pp b LC S EC PAnnotatton : " * Sampl eIndex : 4 8 Sampl eType : Q C Concentration : 0.0 0 ng/m L3.4* 5 Calculate dCone : 1.6 3 ng/m L Acq . Date : 7/21/201 1 ,-. ^ Acq . Time : 1:45:4 1 A M ^^ ^ Modified : Ye s 3.0* 5 Proc . Algorithm : Specif y Parameter s - M Q II I Nois ePercentage : 9 0 Base . Sub . Window : 1,0 0 mi n•^'* ' Peak-Split . Factor : 5 Repor t Larges t

Peak : Ye s 2,6* 5 Min . Peal c Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se e Smoothin g Width : 0 point s 2.4* 5 R TWindow : 30. 0 se c Expecte dRT : 14. 9 mi n2.2* 5 Us eRelativ eRT : N o Int . Type : Bas eT o Bas e2.0* 5 Retentio nTime : 14. 8 ml n- Area : 259017 5 count s ^ 1 o- B Height : 3-40et00 5 cp s j ^ '^' ^ Star t Time : 14. 6 mi n» En dTime : 15. 2 ml ng ^-^ ^ • E 1.4* 5 1.2* 5 1.0* 5 8.0* 4 6.0* 4 4.0* 4 Z0* 4 1 2 5 13. 0 13 5 1 SampleName;T(110720a070
- Sampl elb:-GLHtW)i^- 1 (WI TFile:-k110y20a.wtf r IPea k Name : -PFHS
- Mass(ea) : •399.000^9.00 0 Da,399.O0O«O.0O O Da ' • Comment-DALGW130S0110630{(ai:10)
- Annotatton: " Sampl eIndex : 7 0 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 29. 8 ng/m L Acq . Date : 7/21/201 1 6 0e5
- Acq . Time : 9:27:0 2 A M Modified : " Ye s =c- l Proc . Algorithm : Specif y Parameter s - M Q II I'•'"' ' Nois ePercentage : 5 0 Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 6 5.06 5 Repor t Larges t

Peak : Ye s Mln , Pea k

Height : 0,0 0 ' cp s Min . Pea k

Width : 0.0 0 se c .=^ 5 Smoothin g Width : 0 point s ^'^ ^ R TWindow : 30. 0 se e Expecte dRT : 14. 9 ml n Us eRelativ eRT : No 4.06 5 Int . Type : Bas eT o Bas e Retentio nTime : 14. 8 rain « 3S« S Area : 468383 7 count s fr Height : 6.35e+00 5 cp s > ; Star t Time : 14. 5 ml nf En dTime : 15. 4 ml n| 3-0e 5 2.56 5 2.0* 5 1,Se5
- 1,0e5
- 5,0e 4

1 2 5 13. 0 13 5 14.8 3

14.68 . • 

## km 

# m 

# • m 14. 0 14. 5 15. 0 15, 5 16. 0 16. 5 17. 0 |

Tlme.mi n

148 1

14.67 v 1 i1 

# m 

# m , 14. 0 14. 5 15. 0 1S. 5 16. 0 16. 5 17. 0 | | J l Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , Pag e1 2 o f 2 9 

### Pag e6 5 O f 11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS
. PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

I SampleNama:-\110720a075 - Sampl e ID : "GLP1(K}1-02-ia-016 - Fae:-k110720a,M) r I Pea k Name : -pFKS * Mass^es) : -399.000/99.00 0 Da.399.000/80,00 0 Da - •Comment:-DAL(3W130L0110630{dai:10) - Annotatton: " Sampl eIndex : 7 5 Unknow n Sampl e Type : Concentratio n Calculate d Co i Acq , Date : Acq , Time : N/A 13, 7 ng/m L 7/21/201 1 11:11:5 2 A

M Spe i Nois e Percentage : Base . Sub , Window : Peak-Split
. Factor : Repor t Larges t Peak : Min . Pea k

Height : Mln . Pea k

Width : n g Width : Lf yParamete i

5 0 1.0 0 ni n

R TI I RT : Us e RelaCl > Retentio n "; Area : Height : Star t Time : En d Time : 2.8* 5 2.6* 5 2.46 5 2,26 5 2,065
- 15* 5 1,6* 5 1.4* 5 1.2* 5 1.0* 5 8.0* 4 6.0* 4 4.0* 4

14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 

# I Sampl e Name:-V110720a097 ' Sampl e ID:-GLPllM)1-02 - Pea k Nam* : 'PFHS ' Ma33(as) ; -399,000/99,00 0 Da , Comment:-DA LOW131L011062 2 (d l 1:10) - Annotation : Sampl eIndex : 9 7 e Type : Unknow n itratlon : H/ A ng/m L 

# I 5ampl*Nam*:1(110720a088 - Sampl * 10:-GLP1CM11-02-18«25 - Rle:-K110720a.wt r Pea k Nam* : 'PFHS - M83a(*a} : "399,00099,00 0 Da.399,000/80,00 0 Oa Common t -DA LG W131 S 011062 2 (di l 1:10) - Annotatton : - 1.106 5 1.05*5
- 1.00e 5 950* 4 9.00e 4 8.506 4 8.00e4 < 7.506 4 7.00e 4 6.506 4 a - 6,00e 4 * 55064
- 5 £5J)0e4
- 4,S0e4 - 4,0064 - 3,50e4 - 3,0064 : 2.50e4 - 2,0064 : 1.S0e4 - 1.00e4 - 5000.00 : 0.0 0 Sampl e Tyjje : Concentration : Calculate d Cone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e Base . Sub , Wind o Peak-Spilt . Fac t Unknow n N/ A 5.2 6 ng/m L 7/21/201 1 3:44:4 5 P M N o Specif yParamet e S O rf: 1,0 0 mi n 3r : 2 Repor t Larges t Peak : Ye s Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : 0.0 0 cp s 0.0 0 se c 0 p o 30. 0 se c 14. 9 mi n N o Valle y 14. 8 ml n Area : B4912 3 count s Height : .13et00 5 cp s

12 5 13. 0 

# I SaiT4)leNam6:'V110720al16 rSampl a ID:-GLP1CM)1-02-l&«45 - File:-k l 10720a.wi r Pea k Name : -PFHS - M8ss<6s) : -399.000/99.00 0 Da,399.000/80.00 0 Da ' CommenfDALGW133R0110630(<ai:10) - Annotation: - Sampl e Index : 11 6 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 32. 6 na/m L 7/22/201 1 Sampl e Type : Concentration : Calculate d Cone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e Unknow n N/ A 21. 4 7/21/201 1 6:53:4 7 P M Ye s Specif yP a S O Base . Sub . Window : 1.0 0 Peak-Split . Fac t ar : 3 Repor t Larges t Peak : Ye s Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : 0.0 0 0.0 0 0

Area : Height : Star t Time : En d Time : 338716 5 c 4.69^-^00 5 14. 5 15. 2 6.56 5 Acq . Tim i 1:32:3 5 A M

Peak-Split
. Factor : Repor tLarges tPea l Mln . Pea k

Height : Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte d RT : c p s

Us eRela ( : RT :

14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17, 0 12 5 13, 0 13 5 14, 0 14, 5 15, 0 15. 5 16. 0 16 5 17, 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 1 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 3 o f 2 9 

### Pag e 6 6 of11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# I Samp*6Nam6:-k110720»li rSampl e ID:"GLPKMI^-IWMO " File:T<110720a.«*f r Pea k Name : -PFHS * M3S3(6S} : -399,000/99.00 0 Da.399,000ffl0,00 0 Da ' Comnwnt : ^ALGW133S 0 11063 0 (d a 1:10) - Annotation : " • Sampl eIndex : 11 1 Sampl eType : Unknow n Concentration : N/ A25*5
- Calculate dCone : 66. 9 ng/m L Acq . Date : 7/21/201 1 Acq . Time : 11:47:5 4 P M Z6e5
- Modified : Proc . Algo r Nois ePerc e thm :

tage : Base , Sub . Windo w Peak-split , Repor t Larq i Min , Pea k

H e Min . Pea k

W i R TWindow : Expecte dR

T Int . Type : Retentio nT Area : star t Time : En dTime : Fact o

ight : dth :
RT . Ye s Specif y P 5 0 1.0 0 r : 4 r

0.0 0 0.0 0 30. 0 14. 9 Manua l 14. 8 212815 7 C

O 2 14. 5 15. 1 m mete r

mi n c p s s e c - M

Q

n

n t s m m n n I I I



## 1

% ^ 2.2* 5 2.0* 5 1 8* 5 1.6* 5

12. 5 13. 0 14. 0 14. 5 15. 0 15. 5 16. 0 16, 5 17. 0 

# I Sampl e Name:-k l 10720*084 ' Sampl e ID:-GLP1041-02-18-O2 rFile:K11D720a.wir r Pea k Name : -PFHS " Mas9<6s) : -399.000^9.00 0 Da,399.000ffi0.00 0 Da Comment : "DA LGW131 R 011062 2 (dlll:100) - Annotatton : ~ Satafl eIndex : 6 4 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 297 . ng/m L Acq . Date : 7/21/201 1 Acq . Time : 2:20:4 4 P M 

# I SampleName:n(110720a102 - Sampl e ID:'GLPl(H>1-02-18-035 ' File : Pea k Name : -pFHS " Man<ea) : -399.000/99,00 0 Da,399.000/80.00 0 Da ' Commen t "DA LG W133 L0110S30<d l 1:10) ' Annotatton : ~ Sampl e Index : 10 2 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 47. 3 ng/m L Acq . Pate : 7/21/201 1 Acq . Mod i Pro c Nol s Bas e Pea k Kep o Mln . Mln , Tim e fled : Algorithm : ePe r Su b -Spl l c t L a Pea k hln q R TWindo w Expecte d Us e Int , Ret e Are a ^ela t Typ e tio n Height : Sta t Ti m ventage -

Windo w
, Fact e ges t P e Height : Width : Width : ^T : v eRT : Time : 73 6 9 8:3 8 Y Spe c

r : a k :
3 0 1 N < Va l 1 764 9 ,80 e 1 4 5 2 g t v 5 0 P M P a 1.0 0 3 Ye s 0 0 0 a

. 9

e y

. 8 0 0 0 0

OOS t

. 5

14 5 14. 5 15,0 15. 5 16J ) 16 5 17, 0 

# I 'Sampleriame:-kl10720a125 ' Sampl e ID:-GLPlO«1-02-1(M»ff - FUe : Pea k Name : 'PFHS * Mass(es) : -399.000,«9,00 0 Da,399.000/80.00 0 Da Commen t"DA LGW134 R 0 110628(dll1:100) - Annotatton: " Sampl eIndex : 12 5 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 484 . na/m L Acq . Date : 7/22/201 1 Acq . Time : 4:41:4 9 A M 6.06 5 Modified : Ye s Proc , Algorithm : Specif y Nois ePercentage : 5 0 Peak-Split . Factor : 3 Repor t Larges t

Peak : V

e Mln , Pea k

Height : 0 Min , Pea k

Width : 0 Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 14, 9 Us eRelativ eRT : No Int , Type : Manua l Retentio nTime : 14, 8 Area : 466831 8 C Height : 6.27e-t00 5 Star t Time : 14. 5 En dTime : 15. 2 Parameter s -

s 0 0 cp s 0 0 s e c

ml n c p s ml n rain Mt ' H I



## ^



# 1 45* 5 4.0* 5 3.5* 5 3.0e 5 Modified : Proc . Algor ] Nois ePerce n Base . Sub . W Peak-Split . Repor t Larg e :hm :

:aq e ind o Tac t I t P Min . Pea k

Heigh t Min . Pea k

Width : Smoochin g Width : R TWindow : Expecte dRT : Us eRelativ e Int . Type : Retentio nT i Area : Height : Star t Time : En dTime : RT :

7 5 r e s Specif y Param e 5 0 * : 1.0 0 m l r : 5 a k : Ye s 0.0 0 c 0,0 0 s 0 30, 0 se c 14. 9 mi n No Valle y 14. 8 ml n 529 6 count s .68(,-f00 S cp s 14. 5 mi n 15. 3 mi n

14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 14. 0 .14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , Pag e1 4 o f 2 9 

### Pag e 6 7 O f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b

•Sampl e Name : 1(110720*129 ' Sampt e ID:-GLP10O1-0M8-054 - File : I Pea k Name:-PFHS - Ma8e(*s) : "399,000/99.00 0 Da,399.000/80,00 0 Da ' •Comm*nt:*DAL6W134L0110628(cfil1:10) ' AnnoUtion: " Sampl e Index : 12 9 Sampl e Type : Unknow n Concentration : N/A Calculate d Cone : 104 , ng/m L Acq . Date : 7/22/201 1 Acq , Time : 5:05:4 4 AM Modified : Proc . Algotithn : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Repcir t Larges t

P Min . Pea k

Heigh t Hln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Area : 3 2 Height : Star t Time : En dTime : Ye s Specif y Parame t : 5 0 w : 1.0 0 ml n o r : 3 eak : Ye s : 0-0 0 c p

0.0 0 S t 30. 0 se c 14. 9 ml n No Manua l 14. 8 mi n i890 8 count s I.21et00 6 cp s 14. S mi n 15. 1 mi n

12, S 13, 0 14, 0 14, 5 15. 0 15. 5 16. 0 16. 5 17. 0 

# ( Sampl e Name : 1c110720a15 rSampl e ID : 'GL P 1041^02-18'«69 - File : "k l 10720a.wir r Pea k Nam* : 'PFHS - Ma9s(es) : -399,000/99.00 0 Da,399,000/80.00 0 Da ' Commen t "DA LGW135S 0 110628 ' Annotaflon : " Sampl e Index : 15 2 Sampl e Type : Unknow n Concentration : N/ A ng/m L I SampleNam*:1i110720al38 - Sampl e ID:•GLP1W)1-02-l&4558 rFlle:"kl10720a.wi r 

# I
- . Paa k Nam* : -pFHS - ktaestes) : -399.000/99.00 0 Da,399.000/80.00 0 Da Con¥nent"DALGW134S0110628 - Annotation:" " Sampl e Index : 13 8 cula t Type i N/A Cone : 3.O S i Acq . Date : 7/22/201 1 Acq , Time : 9:14:2 5 A M Modified : Ye s Proc . Algorithm : Specif yPat ; Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 Peak-Split
. Factor : 2 Repor tLarges t Peak : Ye s Min . Pea k

Height : 0.0 0 Mln , Pea k

Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30, 0 S t Expecte d RT : Us e ! RT :

6.23e*00 5 ep s

0. 0 

# I SampteName:-k110720a15 rSampl e ID:-GLP1tM)1.02-1W)74 " File : Pea k Nam* : 'PFHS " Mass(es) : "399.000/99.00 0 Da,399.000/80.00 0 Da ' Comm*nt-DALGW135L0110628(d l 1:10) - Annotatton: TM jampl e Index ; 15 7 Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algo r Base . Sub . Peak-Split . Repor t Lar g thm : 'Ind o 7 7/22 / 2:0 8 Y
Spe c Factor : S t P Min . Pea k

Heigh t Mln . Pea k

Width : Smoothin g W R TWindow : Expecte dR

T dth : Us eRelativ eRT : Int . Type : Retentio nT Area : Height : Star t Time : En dTime : iak :
3 0 1 ' N C Bas e 1 1224576
- .57e * 1 I E 9 7 2 0 u ; 

### „ f v s o P M P a 1.0 0 Ye s 0 0 0

. 9 T o 0 0

. 5
, 5 0 0

B a Sampl eType : Calculate dCone : Acq , Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Unknow n K/ A 71, 9 7/22/201 1 3:53:0 3 P

M Ye s Specif y Pa r 5 0 f . 1.0 0 t : 6 Repor t Larges t

Peak : Ye s Mln . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : 0,0 0 0,0 0 30. 0 3 14- 9 m M o Bas eT o Ba s 14. 9 m Area : 1108024 6 co u Height : ,77e+00 6 c

14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 5 o f 2 9 

### Pag e 6 8 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

isampl*Nam*:-1(110720.166 - Sampl a ID:-GLP1(K)1-02-1IM)79 - Flle:-kl10720a.»lf r I Pea k

Name : 'PFHS ' Ma93(*«> : '399.000/99.00 0 Da,399.000^0,00 0 Da - •Comm*nt:'DALGW13SR0110628(dll1:100) - AnnoUtkin: - Sampl e Index : 16 6 Sampl e Type : Unknow n 148 4 Concentration : N/A Calculate d Cone : 859 . ng/m L1.8* 6 Acq . Date : 7/22/201 1 Acq . Time : 7:01:5 6 PM 1.7B 6 Modified : Ye s Proc . Algorithm : Specif yParameter s - M O II I '-" ^ Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 mi n 1,5* 6 Peak-Split
. Factor : 4 Repor t Larges t Peak : Ye s 14* 6 Mln . pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s '•2* ° R TWindow : 30. 0 se e Expecte d RT : 14. 9 mi n 1.2* 6 Us e Relativ e RT : N o 1.1* 6 Int . Type : Bas e T o Bas e Retentio n Time : 14, 8 mi n . Area : 1315379 5 count s & ^' ^ Height : l,8Set00 6 cp s ^ Star t Time : 14. 5 ml n | fl.0e5 En d Time : 15. 6 mi n g

- 6.0. 5 7.0* 5 6.0* 5 5.0* 5 4.0* 5 

# 1 ^'^ ^ 2.0* 5 1.0* 5 

## ^

1 1 

# 1 ,4.6^ 1 

# • • ^^•- • l i s iT o 135 ' 14. 0 14, 5 15. 0 15, 5 16, 0 16. 5 17. 0

•Samp»eName:T<110720al70 - Sampl e ID : •GLP10^1-02.1(W)84 - Flle:-\110720a.wt r I Pea k Name : -pFHS " hla«s(ee} : "399.000/99.00 0 Da,399.000/80.00 0 Da ' •Commert:-DALGW136L0110630(dil1:10) - Annotatten: - Sampl o Index : 17 0 Sampl e Type : Unknow n 148 2 Concentration : N/A Calculate d Cone : 15. 9 ng/m L Acq . Date : 7/22/201 1 •»•* " Acq . Time : 8:25:5 7 P M 32e 5 Modified : Ye s Proc . Algorithm : Specif yParameter s - M Q II I - - ^ Nois e Percentage : S O ^"* ^ Base . Sub , Window : 1-0 0 ml n Peak-Split , Factor : 4 2.8e 5 Repor t Larges t Peak : Ye s Hln . Pea k

Height : 0.0 0 cp s -fle S Mln , Pea k

Mldth : 0,0 0 se c Smoothin g Width : 0 point s R TWindow : 30. 0 se c2.4e 5 Expecte d RT : 14. 9 ml n Us e Relativ e RT : N o 2.2e 5 Int . Type : Manua l Retentio n Time : 14. 8 mi n - 2.0e5 - Area : 251854 9 count s g " Height : 3.56 e + 00 5 ep s ^ ,_8a5 - Star t Time : 14. 5 mi n T l En d Time : 15. 3 mi n « 1 1.6*5 - 1.4* 5 1,2* 5 1,0e5 ' 8,0e4 - 6,0* 4 4.0*4 - 2.0*4 - 1 1



## 1 14.6 7 I 

# I I • B . l i 5 13, 0 13 5 14. 0 14. 5 15. 0 15, 5 16. 0 16. 5 17. 0 1 S.mpl*Name:K110720al7 rSampl e lD:'GLP1CK>1-02-1»)8 rFil.:"k l 10720..vri r I Pea k

Name : 'PFHS ' Ma«s(*s} : -399,000i«9.00 0 Da.399,000/80,00 0 Da ' •Comment-DALOW13eS0ll0630<dll1:10) - Annotatton: " Samril e Index : 17 9 Sample•Type : Unknow n Conoentration : N/ A Calculate d Cone : 25. 9 ng/m L54* S Acq . Date : 7/22/201 1 Acq . Time : 11:35:0 1 P M 5,2e 5 Modified : Ye a *°* ^ Proc . Algorithm : Specif yParameter s - M Q II I 4.8* 5 Nois e Percentage : 5 0 Base , Sub , Window : 1,0 0 mi n ^-^ ^ Peak-Split
. Factor : 3 4,4* 5 Repor t Larges t Peak : Ve s Min . Pea k

Height : 0.0 0 cp s 4.2* 5 Mln . Pea k

Width : 0.0 0 se c40, 5 Smoothin g Width : 0 point s R TWindow : 30. 0 se c3,8* 5 Expecte d RT : 14. 9 ml n •»«.« ; Us e Relativ e RT : N o ^'^ ^ 3.4* 5 Int . Type : Valle y Retentio n Time : 14. 8 ml n - •'•^ ' Area : 407719 6 count s fr3.0* 5 Height : 5,60e.00 5 ep s ^ l Star t Time : 14. 5 ml n H ^'^ ^ En d Time : 15. 2 ml n | 2,6* 5 2.4* 5 2,2* 5 2.0* 5 1,8* 5 1,0* 5 1.4e 5 1.2e 5 1.0e 5 8,0e4 - 6.0* 4 4.0* 4 Z0* 4

•li s 13. 0 13. 5 |SampleName:1<110720«184 - Sampi e ID : "GLt>1CM)1.02-l&W3^ FU*:-kl10720a.wi r I Pea k

Nam* : -PFHS - Ma8a(*a) : -399.000^9.00 0 OB.399.000«0.00 0 Da " Sampl e Index : 184 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 0,081 4 na/m L. g^ . Acq - Date : 7/23/201 1 Acq . Time : 1:20:0 3 A M Modified : N o Proc . Algorithm : Specif yParameter s - M Q II I 1-7* 4 Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 ml n 1.6*4 - Peak-Split
. Factor : 2 Repor t Larges t Peak : Ye s ^ 5^4 . Min . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se c, ^ . Smoothin g Width : 0 point s ''^ ^ R TWindow : 30. 0 se c Expecte d RT : 14. 9 ml n '•3» * Us e Relativ e RT : N o 1.2* 4 Int . Type : Bas e T o Bas e Retentio n Time : 14. 8 ml n « 1.1B4 - Area : 14524 6 count s g " Height : 1.96e-t00 4 cp s > L1 o«4 . Star t Time : 14. 6 mi n 1 En d Time : 15. 0 ml n | g^„ - 6000. 0 7000. 0 6000,0 - 5000. 0 4000.0 - 3000. 0 2000.0 - 1000.0 - 12 5 13. 0 13. 5 146 0 ' 1



### i \ 

# j 

# J

14. 0 14. 5 15. 0 15, 5 16. 0 16, 5 17, 0

Tim* , mi n 1

148 9 I I



## L 

# 1 I 14. 0 14. 5 

# 1 1 , , J 15. 0 15. 5 16. 0 16. 5 17, 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 6 o f 2 9 

### Pag e 6 9 o f11 3GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

*Nam*:1<110720a19 rSampl e ID : •GLP10O1-02-1B-098 - Flle:"k110720a.wi r Pea k Name : "PFHS ' Mass{e9} : -399.000/99,00 0 Da,399.000/80.00 0 Da Comment : 'DA LG W13 A0 110624 ' Annotatton : ~ Sampl e Index : 19 3 Sampl eType : Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Base . Sub , Windo v unknow n N/ A 0.10 6 ng/ m 7/23/201 1 4:28:5 7 A

M Ye s Specif y Parame t 5 0 : 1.0 0 ml n Peak-Split . Factor : 4 Repor t Larges t

Peak : Ye s Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Atea : - 1 Height : i Star t Time : En dTime : 0.0 0 c p 0.0 0 s e 0 P

C 30. 0 se c 14. 9 ml n N o Bas eT o Bas e 14. 8 mi n 487 2 count s .See+00 4 cp s 15. 0 mi n 2.3*4
- 2.2* 4 2. 1 e4 2.0* 4 1,9* 4 1,8* 4 1.7* 4 1.6* 4 1.5* 4

; 1.3* 4 1.2* 4 1.1 * 1J)»4
- 9000. 0 6000. 0 7000. 0 6000. 0 5000.0
- 4000.0
- 3000. 0 2000,0
- 1000,0
- 0, 0 »Hni'*""**»*''?"^^W O 12. 5 13. 0 V^t«wMMi!V ^ *f^^Mt4,^W^4J*^ ^

13. 5 14. 0 14. 5 15J ) 15. 5 16. 0 16. 5 17, 0 

# I Sampl e Name : T<110720s20 rSampl e ID : -6LP1CM)1-02-18-108 " File : 1(110720a.wir r Pea k Name : -PFHS - Mass(es) : -399.000/99.00 0 Da.399.000/80.00 0 Da Comment:-DALG W138 L0110630 - Annotation: - Sampl e Index : 20 7 Sampl e Type : Unknow n c.„„„.„.,o,. : », A^ Z4e 4 2.3e 4 M^ i II I 2.2* 4

2.1e 4 2.0e 4 1.9e 4 1.8* 4 1.7* 4 1.6*4 - 1.5*4 - Mols i 5 0

. Kin ! Peak-Split
. Factor : 3 Repor tLarges t Peak : V* Mln . Pea k

Height : 0 . Mln . Pea k

Width : 0 , Smoothin g Width : 0 R TWindow : 30. 0

I RT : Relativ e RT : 1 4 . 9 

# I SampleNam*:-k110720a19 rSampl * ID:'GLP10«1-O2-18-103 - Flle:-k110720a.wt r Paa k Name : 'PFHS - Masa<*8) : -399.000/99.00 0 Da.399.000/80.00 0 Da ' Comm*nt-DALGW138R0110630(dil1:10) " Annotation; - Sampl e Index : 18 8 Sampl e Type : Unknow n 7.Se S 7.0e 5 6.5* 5 6,0eS 5,5e5 - 5.0e 5 4.5eS 4.0eS ; 3.5e5 - 3.0e5 - 2.5e5 - ZOeS 1.SeS 1.0eS la l Time : 14. 8 Area : 611955 5 co i Height : 8.01etOO 5 Star tTime : 14. 5 En d Time : 15. 4

14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 

# I Sampl e Name:-Vt10720a2i rSampl e ID:'GLPICMJI-02-18- 1 Pea k Name : 'PFHS ' Mas9{es} : "399.000/99.00 0 Da,399.0a0/80
. Comman t 'DA LOW138 S 011063 0 (d O 1:10) - Annotation : - Sampl e Index : 21 2 Sampl e Type : Unknow n Calculate dCone : Acq . Date : Acq , Time : Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Repor t Larges t

P Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int , Type : Retentio nTime : Area : 1 Height : Star t Time : En dTime : 0,11 2 n g 7/23/201 1 9:22:5 4 A

M Y- a Specif y Para m 5 0 eak : Ye s

0-0 0 0,0 0 0 30, 0 se c 14. 9 mi n N o Manua l 14. 3 mi n 94 4 6 5 count s 2.5£e+00 4 cp s 14. 5 mi n 15. 0 mi n 1.4*4 - 1.3* 4 1.2* 4 1.1*4 - 1.0*4 - 9000.0 - 8000.0 - 7000.0 - 6000.0 - 5000,0 - 4000.0 - 3000. 0 2000. 0 1000. 0 0. 0 •cif y Parameter s - M Q II I Base . Sub
. Peak-Split
. Repor tL a r e

366187 5 count s

4.47e+00 5 cp s 14. 5 mi n 15. 3 mi n

irt,ii|ti| i jiMi |.i ^

\>^,,iiy'^mi^)^^¥\Hu_JUK^'fMmitt^M^^ ^ 13. 5 14, 0 14. 5 15, 0 15. 5 16. 0 16. 5 17. 0 14. 0 14. 5 15. 0 1 S. 5 16. 0 16. 5 17. 0 Dat aprinte db y ST W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 7 o f 2 9 Pag e 7 0 O f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k 

### Result s Name : kll0720a.rd b

Sampl * Nam* : "kl10720B221 ' Sampl * ID : •GLP1(H31-02-18-118
- FIIe:1(110720a.«*(i r P*a k Nam* : "PFHS " MBS9<*S) : -399.000/99.00 0 Da.399.00affl0.00 0 Da ' Comman t

-DALG WCVU26

C 0 11062 2 (d a1:10) ' Annotatton : * * Sampl e Index : 22 1 Sampl e Type : Unknow n Concentration : K/ A2.8*5
- Calculate dCone : 68. 3 ng/m L Aeg , Date : 7/23/201 1 Acq , Time : 2:16:5 2 P M 2.6* 5 Modified : Ye a Proc , Algorithm : Specif yParam e Nois e Percentage : 5 0 Base , Sub , Window : 1.0 0 m : Peak-Split . Factor : 3 Repor t Larges t Peak : Ye s te r n Min . Pea k Height : 0.0 0 cp s Min , Pea k Width : 0.0 0 s Smoothin g Width : 0 R T Window : 30. 0 se e Expecte d RT : 14, 9 ml n Us e Relativ e RT : N o Int . Type : Manua l Retentio n Time : 14, 8 ml n Atea : 217157 9 count s Height : 2.82e*00 5 cp s Star t Time : 14. 5 ml n En d Time : 15. 2 mi n po i - M Q II I

t s



## » s 2.2e 5 2J)e 5 1.8* 5

Sanrqil«Nam*:1(110720a237
- Sampl e lD:'Gt.PiaO1-02-18-128
- FUe : Pea k Name : -PFHS
- ltfaaa(*s} : *399.000/99.00 0 Da,399,000/80.00 0 Da ' Cwnment : *DA

L6W140 R011062 2 (cQ 1:10)
- Annotation : ~ Sanpl e Index : 23 7 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : 17. 7 ng/m L Acq . Date : 7/23/201 1 •Samp U Name : 1(110720*226 - Sampl * ID:'GLP1(K)l-02-18-123 - File : •Pea k

Name : 'PFHS * Mass(ea} : '399.000(99.00 0 Da.399.000/80.00 0 Da ' • Comment'DALGWCW26L0110622{tlII1:10)
- Awiotatkin : * Sampl e Index : 22 G Sampl e Type : Unknow n Acq , Date : Acq . Time : Modified : Proc . Algor i Nois e Perce n Base . Sub , W Peak-Split . Repor t Larg e Min . Pea k H e Min . Pea k W i 7/23/201 1 4:01:3 9 P M N o Specif yPara m 5 0 ndow : 1.0 0 m •ac t r : 2 S t Peak : Ye s gh t Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e Int . Type : Retentio n T l Area : Height : Star t Time : En d Time : BT : 0,0 0 0,0 0 0 30. 0 se c 14. 9 ml n M o Valle y 14. 8 ml n 328767 9 count s .04e+00 5 ep s 14. 5 ml n 15. 2 mi n

12 5 13. 0 

# I San^Nanrt*:'k110720a24ff
- Sampl * ID:-GLP1MI1-02-1S-135
- FUe:-k110720a.w« r P*a k Name : -PFHS
- Masa(es) : -399.OOCV99.00 0 Da,399.000/80.00 0 Da ' Comment'DA LGW141 R0110630
- Annotation:
- Sampl e Index : 24 6 Unknow n N/ A 6.5e 4 6.0e4
- 5.5*4
- 5.0*4
- 4.5*4
- 4.0*4
- 3.5*4
- 3.0*4
- Acq . Tlm i 7:52:1 6 P M Modified : Y Proc . Algorithm : Spe c Nois e Percentage : Base . Sub . Window : Peak-Split . Factor : Repor t Larges t Peak : Min . Pea k Height : Mln . Pea k Width : Smoothin g Width : R T Window : 3 0 s f yPara m 5 0 1.0 0 m 4 Ye s 0,0 0 0.0 0 0 0 se c Expecte d RT : 14. 9 ml n Us e Relativ e RT : N Int . Type : Bas e Retentio n Time : 1 Area : 280091 1 Height : 3.76 e T o Bas e

. 8 mi n 00 5 cp s Calculate d Con e Acq . Date : Acq . Time : Modified : Proc . Algorith m Mols e Percenta g Base . Sub . Klnd c Peak-Split . Fa c Repor t Larges t 0.29 8 ng / 7/23/201 1 11:01:17 Ye s Specif y : S O w : 1. 1 eak : Y

i Mln . Pea k Height : 0 Mln . Pea k Widt h Smoothin g Widt h R T Window : Expecte d RT : Us e Relativ e R T Int . Type : Retentio n Time : Area : Height : Star t Time : En d Time : 0 0 30. 0 14. 9 N O Manua l 14. 8 8793 9 c 6.68e'f00 4 14. 6 15. 1 P M
Param e 0 m l 0 0 c DO 3 mi n

ml n cp s ml n ml n

. 0 14. 5 15. 0 15. 5 16J ) 16. 5 17. 0 12. 5 13. 0 16. 5 17. 0 

### Dat aprinte dby ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , Pag e1 8 o f 2 9 Pag e 7 1 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

•Sampt*Nwn*:T<110720a251 ' Sampl e ID : •GLP1(M>1-02-18.140 ' File : •Pea k Name:-PFHS - Masa{e») : "399.000/99.00 0 Da,399.000/80.00 0 Da ' •Cominent-DALGW142R0110622(dil1:10) ' Annotatk>n: - Sampl eIndex : 25 1 Sampl eType : Unknow n Conetntration : N/ A Calculate dCone : 10. 0 ng/m L Acq . Date : 7/24/201 1 Acq . Time : 12:46:2 9 A M Modified : Y 3 Proc . Algorithm : Specif yP a Nois e Percentage : Base . Sub , Window : Peak-Split
. Factor : Repor t Larges t Peak : Mln . Peak

Height : Mln , Pea

k Width : Smoothin g Width : R TWindow : 3

0 5 0 1.0 0 3 Ye s 0.0 0 0.0 0 0 0

Atea : Height : Star t Time : En dTime : 159907 0 coun t

2.13et00 5 c p 14. 5 m i 15. 3 m i

Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , Pag e1 9 o f 2 9 

### Pag e 7 2 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

• Sampl eNam*:'K110720a018
- Sampl * ID:'11003-2ft«
- Fl*:-W10720a.v«« r • Paa k Nama:-pFOS
- Ma3S<es):-499.000^.00 0 Da.499.000W>.OOODa,499.000/130.000Da
- • Comment : *2. 5 r^fm LF C at dI nM mQ Wat* ^ Annotation: " Sampl eIndex : 1 8 Sampl eType : Standar d Concentration : 2.5 0 ng/m L Calculate dCone : 2.4 0 ng/m L Aeg . Date : 7/20/201 1 Acq . Time : 3:17:0 5 P M

Base . Sub . Window . Peak-Split . Facto J Repor t Larges t

Pe , Mln . Pea k

Height : Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : • Sampl * Nam*:'V110720a028 ' Sampl * ID:-SoN*n t BfanlTFlle:-k110720a.ni r • Pea k Name:'PFOS ' lrtass{**):'499.000/99.00 0 Da.499.000/SO.OOODa,499.000/130.000Da
- • Comn*nt'VllQWatarTN11-023 r AnnoUtlon:* " Sampl eIndex : 2 8 Sampl eType : Unknow n Concentration : N/ A Calculate dCone ; 0.019 1 ng/m L Acq . Date : 7/20/201 1

13. 5 14. 0 1 5 5 16. 0 16. 5 17. 0 17. 5 16. 0 

# I SamplsName:1(110720a029
- Sampl eIO:''LC^1l0714- r Fl*:-kll0720a . Paa k Nam* : 'PFOS ' Massfes) : -499.00099.00 0 Da.499.000/80.00 0 Da,499,000/130.00 0 Da
Comment : t}.5pp b LCS ' Annotation : ~ Sampl eIndex : 2 9 Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e Base . Sub . Wind o 6:46:2 6 N o Specif y 5 0 P M para m •f : 1.0 0 m Peak-Split . Factor : 2 Repor t Larges t P Mln . Pea k Heigh t Min . Pea hWidth : Smoothin g Width : B T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Area : Height : Star t Time : En d Time : ak : Y 0 0 0 30. 0 15. 9 N o Valle y 15. 8 116 7 c .90efO 0 15. 7 15. 9 0 0 0 0 se c mi n
mi n cp s mi n mi n

13. 5 14. 0 1 6 5 17. 0 Sampl eName:-kll0720a032 ' SamplelD:-LCS-110714-4 * Fle:-k110720a.vri r Pea k Name : 'PFOS * M8a3(eB) : '499.000/99.00 0 Da,499,000/80.00 0 Da,499.000/130.00 0 Da ' Commen t

"Spp b LCS
- Annotatton: " Sampl eIndex ; 3 2 Sampl eType : O C Concentration : 4.9 8 ng/m L Calculate dCone : 4.9 3 ng/m L5,ZB5
- Acq . Date : 7/20/201 1 5 0*5
- 4.8*5
- s - M QII I^-^•5
-

4.4*5
- 4.2*5
- 4.0*5
- 3.8*5
- 3.6*5
- 3.4*5
- 3.2e5
- 3.0e5
- H2.8*5
- I2.6a5
- I2.4a5
- 2.2e5
- 2.0*5
- l.ae5
- 1.6e5
- 1.4e5
- 1.2e5
- 1,0*5
- 8,0*4
- 6.0*4
- 4.0e4 ' 2.0* 4 Sampl e Type : Concentration : Calculate d Cone : Acq . Date : Acq . Tine : Modified : Proc . Algorithm : Nois e Percentag e Base . Sub . Wind o Peak-Split . Fac t Repor t Larges t P Min . Pea k Heigh t Min . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Q C 0.49 8 nq/m L 0. 4 2 7 ng/m L 7/20/201 1 7:07:2 1 P M Ye s Specif yParamet e 5 0 J : 1.0 0 mi n ;r : 1 -ak : Ye s

0.0 0 ep s 0.0 0 3« C 0 p o 30. 0 se c 15. 9 ml n N o Valle y 15. 9 ml n Area : 3053 4 3 count s Height : Star t Time ; En d Time : .47e+00 4 cp s 15. 7 mi n 16. 1 ml n Acq . Time : Modified : Proc . Algorithm : Nois e Percentage : Base . Sub . Windo w Peak-Split . Fact o Repor t Larges t P e Mln . Pea k Height : Min . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : 8:10:1 2 P M N o Specif yP a : 1.0 0 r : 2 ak : Ye s 0.0 0 0.0 0 0 30. 0 15. 9 n N o Valle y 15. e r

. Area : 351053 9 cou r Height : 5 Star t Time : En d Time : 31etO0 5 c IS. 7 K 16. 4 R

0. 0 15. 0 15. 5 16. 0 1 6 5 17. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e2 0 o f 2 9 

### Pag e 7 3 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# I Sampl e Nam*:-1(110720*035 ' Sampl e ID:-LCS-1107l4- rFle:-k110720a.vrit r P*a k Nam* : -PFOS " M8ss{*s} : '499.00CV99.00 0 Da,499.000/80.00 0 Da.499.000/130.00 0 Da ' Comment : "SOpp b LCS ' Annotation ; ~ Sampl e Index : 3 5 Sampl e Type : Q C Concentration : 2 9. 9 ng/m L Calculate d Cone : 30. 5 ng/m L Acq . Date : 7/20/201 1 Acq . Time : Modified : Proc . Algorithm s Nois e Percentage : Base . Sub . Windo

w Peak-Split . Fact o 9:13:0 6 Ye s Specif y 5 0 P M Pa r 1.0 0 c : 1 Repor t Larges t

Peak : Y

( Min . Pea

k Height : Mln . Pea

k Width : Smoothin g Width : R T Window : Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : Area : 20

4 Height : 2 Star t Time : En dTime : 0 0 0 30. 0 15, 9 M o Manua l 15, 8 379 6 95e*00 ' 15, 7 16. 4 O U 0 0 3

CO U meter s mi n c p s s e e pol n

t s m m n

13. 5 14. 0 15. 0 15 5 16. 0 16. 5 17. 0 17. 5 18. 0 Sampl e Nam e :-k110720a045 - Sampl e ID:-LCS-110714-i rFlle:-kll0720a.w(r r Pea k Name : -PFOS * Masa(*>) : "499.000/99.00 0 Da.499.000/80.00 0 Da,499.00(V130.00 0 Da ' Comment : ^p b LC S ECF " Annotation : ~ Sampl e Index : 4 5 Sampl e Type : Q C Concentration : 4.9 6 ng/m L Calculate dCone : 4.1 4 ng/m L Acq . Date : 7/21/201 1 2.8e 5 Acq . Time : 12:42:4 9 A M Modified : Ye s Ptoc . Algorithm : Specif yParamete i Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 mi n Peak-Split
. Factor : 4 Repor t Larges t

Peaki 

# I SamplaName:-kl10720a042 ' Sampl e ID:'LCS-110714-10 - File:-kl10720a.wt r Pea k Name : -pFOS " Masa<ea} : '499.000/99.00 0 Da.499.000/80.00 0 Da.499.000/130.00 0 Da ' Commen t"O.Spp b LC S EC P Annotatton : ~

3.2e 4 3.0e 4 2.6e 4 2.6* 4 2.4* 4 2.2* 4 2.0* 4 1,8* 4 1.6* 4 1.4* 4 1.2*4 1.0*4 ' 8000. 0 6000. 0 4000. 0 2000.0
- ..^mtt .

i o ^ ,MeiyS.m.. . 1S. (

i .l.^'M. J M|i> i I I i<i4ii'^r''T*^*f''*nitifttih>'rlHt * 15, 0 15. 5 16. 0 16 5 17. 0 17 5 18. 0 

# I San:v<«NanM:-V110720a048 - Sampl e ID:'LCS-110714-16 - Flle:1(110720a.wt r Pea k Name : "PFOS " IAia3(ea) : -499.000/99.00 0 Da.499.000/80.00 0 Da.499.00a/130.00 0 Da ' Commen t -30pp b LC S EC P Annotation : ~ Sampl e Index : 4 6 Sampl e Type : Q C Concentration : 29. S ng/m L Calculate d Cone : 29- 4 ng/m L Acq . Date : 7/21/201 1 Acq . Time : 1:45:4 1 A M

, Algo j .age : 5 0 Height : Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : ep s

I Rel a •RT : N o

Manua l le : 15. 8 rain 29550 4 9 count s 2.93et00 5 cp a Base . Sub
. Window : Peak-Split , Factor : 2 Repor tLarges t Peak : ' Min , Pea k

Height : ( Mln . Pea k

Width : ( Smoothin g Width : ( R TWindow ; 30. 0 Expecte d RT : 5 . 9 ! Rel i ! RT :

15, 0 15. 5 16. 0 16. 5 17. 0 17. 5 18. 0 15. 0 15. 5 16. 0 16. 5 17. 0 17 5 18. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 / 201 1 Pag e2 1 o f 2 9 

### Pag e 7 4 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# I SampleName:-k110720e061 - Sampl e ID:'GLP1CKI1-02-18-006 - Flle: 1 Pea k Name : 'PFOS ' Ms3a(*a) : -499.00af99,00 0 Da.499.000/80.00 0 Da.4 9 Comment:'DALGW130R0110630((£l1:100) - Annotatton: ' Sampl e Index : 6 1

Type : Unknow n 2.2* 5 2.1* 5 2.0* 5 M O II Il-9^
-

1.8* 5 1.7* 5 1.6e 5 1.5* 5 1.4* 5 1.3* 5 & 1-2» 5 #1.1* 5 I1.0e5
- 9.0*4 - 8.0* 4 7.0*4 - 6.0*4 - 5.0*4
- 4.0e4
- 3.0e4
- 2.0*4
- 1.0*4 - Sampl eType : Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Repor t Larges t

P Min . Pea k

Heigh t Min . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Area : 3 1 Height : Star t Time : En dTime : Unknow n N/ A 440 . n g 7/21/201 1 e:18:o eA

M Ye s Specif y Para m 5 0 >< : 1.0 0 m 3 r : 2 lak : Ye s O.O O 0.0 0 0 30- 0 se c 15. 9 ml n N o Manua l 15. 7 ml n 030 8 count s .21et00 5 cp s 15. 4 mi n 16. 1 ml n

0. 0 13 5 14. 0 

# I Sampl e Nam*:-k110720a075 - Sampl e ID:-GLP1(K)1-«2-18-016 - Rte:-V11D720a.wt r Pea k Name : "PFOS ' M833(es) : '499.000199.00 0 Da,499.000ffl0.00 0 Oa,499.00(V130.00 0 Da " Comment:'DALGW130L0110630(dill:10) ' Annotation ; " Sampl eIndex : 7 5 SampleNama:'V110720a070 ' Sampl e ID:'GLP1fr01-02-18-011 - FUe: 1 

# I P*a k Nam* ; 'PFOS * Mass(e9} : '499.000/99.00 0 D8,499.000n0.00 0 Da,4 ! Conm»ent-DALGW130S0110630(dil1:10) ' AnnoUtlon:" ' Sampl eIndex : 7 0 5.2* 5 5.0*5
- 4.8*5
- 4.6e5
- 4.4*5
- 4.2e 5 4.0e5 < 3,8e 5 3.6*5
- 3.4e5
- 3.2*5
- 3.0*5
- 2.Be5
- 2.ee5
- 2.4* 5 2.2e 5 2.0* 5 1.8* 5 1.6* 5 1.4* 5 1.2* 5 1.0*5
- 8.0*4
- 6.0*4
- 4.0* 4 2.0* 4 0. 0 Acq . Dat e Acq . Tim e Modified : Proc . Algorith m Nois ePe r Base . Su b Peak-Spl I Repor t L a Min . Pea k Min . Pea k R TWindo w Expecte d Us eRela t Retentio n Area : Height : Star t Ti m 7/21/2 0

9:27:0 2 Ye s Specif y centage : 5 0

. Fa c rges t Heig h Widt h Widt h T : v eR T Time : w : 1 . o r : 6 1

A M Param e 0 m l eak : Ye s : 0

0 0 30. 0 15. 9 No Bas eT o 15- 7 707081 7 0 0 c

0 0 3 rain Bas e 5.24e+00 5 cp s 15. 3 ml n

15. 0 15. 5 16. 0 16 5 17. 0 17. 5 18. 0 

# I Samp*eName:T(110720a06 ySampl e ID : •GLP1(M)l-02-18-025 - FU e :-kl10720 a .wH T Pea k Name : -PFOS - Mass(*s) : '499.000/99.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da Comment-OALGW131SO11O622{d01:1O> " AnnoUtfan: - ampl e Index : 8 8 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 62. 0 ng/m L Acq . Date : 7/21/201 1 Acq . Time : 11:11:5 2 A M Proc . Algorithm : Specif y Parameter s - M O II I Nois ePercentage : 5 0 Base . Sub . Window : 1.0 0 mi n Peak-Split . Factor : 8 Repor t Larges t

Peak : Ye s Min . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s R TWindow : 30. 0 se c Expecte dRT : 15, 9 rain Us eRelativ eRT : N o Int . Type : Bas eT o Bas e Retentio nTime : 15, 8 ml n Area : 440259 3 count s Height : 3.36at00 5 cp s En dTime : 16. 4 ml n 

## A 

# 1 3.2B 5

2.4 ^

Z0e 5 1.8e 5

1.4e 5

4.0* 4 2.0* 4 Concentr a Calculat e Acq . Dat e Acq . Tim e Modified : Ion :

Con e

Proc , Algorith m Mois ePe r Base . Su b entag e Unkr. c w n H/ A 5 3 1 7/21/2 0 3:4 4 4 5 Ye a Spe c Window : Peak-Split , Fac t Repor t L a Min . Pea k Min . Pea k Smoothin g R TWindo w ges t

1 Helgh i Widt h Widt h Expecte dRT : Us eRela t Int . Typ e Retentio n Area : Height : v eRT :

Time : 

## ; Star t Time : En dTime : o r :

eak :
3 0 1 5 Bas e 1 5 3033 6 f y 9 0 1 P M Par a 1.0 0 3 0 

## ( ' 0 0

. 9 T o

. h 3.37et0 0 1 5 1 6 . 4

. 0 0 0 s e m i Bas e m l c p m i mi

15. 0 15. 5 16. 0 16. 5 17. 0 17 5 18. 0 I^T ' I Vi'nri-'*«***<^***«-JVS**''* V 15. 0 15. 5 16. 0 16 5 17. 0 17. 5 18. 0

TTme . m h Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 2 o f 2 9 

### Pag e 7 5 O f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# I Sampl*Nam*:-k110720a09 rSampl e ID:-6LP1CM1-02>16<I30 - Fi k Pea k Nam* : -PFOS ' !*««(•») : •499.000«9.00 0 Da,499.000/80.00 0 Da, < Commen t -DA LG W131 L0 11062 2 (d U 1:10) ' Annotation: ' Sampl e Index : 9 7

Per t N/ A

;hm : Specif yPa i Sub
. Window : 1.0 0 Peak-Split
. Factor : A Repor t Larges tPeak : Ve s Min . Pea k

Height : 0.0 0 Mln . Pea k

Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30. 0 s t Expecte d RT : 15. 9 m J Js e Re l ! RT :

2.14et00 5 cp s 2.1* 5 2.0e 5 1.9* 5 1.8e S 1.7e5 - 1.6e5 ' 1.5e 5 1.4* 5 1.3* 5 1.2* 5 1.1* 5 1.0e5 - 9.0*4 - 8.0*4 < 7.0*4 - 6.0*4 - 5.0*4 - 4.0*4 - 3,0*4 - 2.0*4 - 1.0*4 - 15. 0 15 5 16. 0 16. 5 M O 17 5 18. 0 

# I Sampl*Nam*:-k110720allff ' Sampl a ID:'GL P 10-01-02- 1 P*a k Nam* : "PFOS " Ma33(fls) : "499.000/99.00 0 Da.499. a Comment-DALGW133R011063D(dll1:10) - Annotation : Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : 44. 1 ng/m L Acq . Date : 7/22/201 1 Acq . Time : 1:32:3 5 A M Modified : Proc . Algorithm : Nois ePercentag e Base . Sub . Wind o Peak-Split . Fac t Repor t Larges t

P Mln . Pea k

Heigh t Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Ye s Specif y 5 0 Para m J : 1.0 0 m :.r : 2 -ak : Ye s

0 0 0 30. 0 15. 9 No Manua l 15. 7 Area : 314563 4 c Height : 2.45et00 5 Star t Time : En dTime : 15. 3 16. 1 0 0 0 0 s e e

ml n ount s c p s ml n mi n 8-045 " Fll»: 1 .OOODa. 4

2.4* 5 2.3* 5 2ito 5 Zl* 5 2.0*5 - 1.9*5 - 1.8*5 - 1.7* 5 1.6*5 ' 1.Se5 1.4*5 - 1.3*5 - 1.2e 5 l.le S 1.0e 5 g.0e 4 e.0e 4 7.0e 4 6.0*4 ' 5.0* 4 4.0* 4 3.0* 4 2.0* 4 1.0e4 - 0.0 - 13. 5 14. 0 15. 0 15. 5 16. 0 16 5 17, 0 17. 5 18, 0 

# [ SampleMame:'V110720a102 ' Sampl e ID:-GLP1&O1-02-18-O35 - File:-kl10720a.wlt r Pea k Name : "PFOS - hlaa3(*t} : '499.000A9.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da " Comment-OALGW133L0110630{dl1:10) - Annotatkin: - an^l e Index : 10 2 

# I SampleName:'V110720a1i rSampl e ID:"GLP1(K)1-02-18-O40 - File ; Pea k Name : -PFOS - htasa(**) : '499.OOCV99.000 Da,499.000/80.00 0 Da , Comment:-DALGW133S0110630{dai:10} - Amotation: - Sampl eIndex : 11 1 Sampl eType : Unknow n Concentration : N/ A Calculate d Cone : 196 . ng/m L19* 5 Acq . Date : 7/21/201 1 Acq . Time : 11:47:5 4 P M 9 - M

O II I1-7* 5

1.6*5 1.5*5 I t s '-^ ^

1.3* 5 1.2* 5

^ 1.0* 5 I 9.0* 4 8.0*4 - 6.0*4 5.0*4 4.0*4 ' Modified : Ptoe . Al g Nois ePe r Base . Su b Peak-Epl l Repor t L a Min . Pea k Min . Pea k Smoothin g R TWindo w Expecte d Int . Typ e Retentio n Area : Height : Ye s

jrithm : Specif y P a rentage : 9 0 Window : 1.0 0

. Factor : A rges t Peak : Ye s Height : 0.0 0 Width : 0.0 0 Width : 0

30. 0 a T : 15. 9 n v eRT : No

Bas eT o Ba; 3 Time : 15. 6 n 2B0629 5 co u 1.97e+00 5 c Sanpl eTyp e Coneentratl i Unknow n

n : N/ A Calculate dCone : 137 . n Acq . Date : Acq . Time : Modified : Proc . Algor : Nois ePerce i Base . Sub . W Peak-Split . Repor t Larg e 7/21/201 1

B:3B:5 2 P

M Ye s thm : Specif y Par a tage : 5 0 indow : 1.0 0 Factor : 5 S t Peak : Ye s Hln . Pea k

Height : 0.0 0 Mln . Pea k

Width : 0.0 0 Smoothin g Width : 0 R TWindow : Elxpecte dRT : Us eRelativ e Retentio nT l Area : Height : Star t Time : En dTime : 30. 0 s e 15. 9 m i RT : N o Bas eT o Bas e me : 15. 7 mi 968676 0 coun t 7.27e+00 5 c p 15. 3 m i 16. 4 ml 1 3.5* 5

2.5e5 - ZOe S

15. 0 15 5 16. 0 16. 5 17, 0 17 5 18. 0 13 5 14. 0 15. 0 15. 5 16J ) 16 5 17. 0 17 5 18. 0 

### Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 3 o f 2 9 201 1 

### Pag e 7 6 O f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

•SamplaName:T<110720a084 - Sampl e ID : •GLP1(M1-02-1ftO21 - File;-k110720a.*yt r •Pea k Nam* : "PFOS ' Mass(*B): '499.00099.00 0 Da,499.000/80.00 0 Da.499.00a'130.00 0 Da ' •Comm*nt:'DALGW131R0110622(dai:100) - Annotatton:" ' Sampl e Index : 84 ! Type : Unknow n Concentr e N/ A .4B0 .

Base . Sub . Window : Peak-Spilt . Factor : Repor t Larges t

Pea k Min . Pea k

Height : Kin . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT :

En dTime : :lf y Pa i 5 0 1.0 0 6.0* 5 75* 5 7.0* 5 6.5* 5 6.0* 5 55* 5 5.0* 5 4.5* 5 4.0*5
- 3.5*5
- 3.0*5
-

15. 0 15 5 16. 0 16. 5 17. 0 17 5 18. 0 

# I SampleNam*:-k110720a129 - Sampl e ID:'GLP1001-02 - Pea k Nam* : 'PFOS ' Ma3s(*s) : "499.000/99.00 0 Da,499. a Comment:'DALGW134L0110626(d a 1:10) - Annotatki n Sampl eIndex : 12 9 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 438 . ng/m L Acq . Date : 7/22/201 1 Acq . Time : 6:05:4 4 A H Modified : Ye s Proc . Algorithm : Specif y Parameter s - H Q II I Base . Sub . Windo w Peak-Split . Facto i Repor t Larges t

Pe i Mln . Pea k

Height : Kin . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : •18-054 - Fllo: 1 ,000/80.00 0 Da.4 l 

# I SaiT^Nam*:-k110720sl25 - Sampl * ID:-GLP1M}1-O2-18-0G0 - Fa*: ' Paa k Nam* : -pFOS * Masst**) : '499.000/99.00 0 Da.499.000/80.00 0 Da,4. ' Connman t 'DA LG W134 R 011062 8 (dl l 1:100) ' Annotation: ' Sampl eType : N/A Acq , Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentage : Base . Sub . Windo w Peak-Split . Fact o Repor t Larges t

P e Min . Pea k

Height : Min . Pea hWidth : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : 7/22/2 0 4:41:4 9 Ye a 5 0 1 A M Param e 1.0 0 ml a k : Y 0 0 0 30. 0 15. 9 No Manua l 15. 7 Area : 701180 3 Height : 5 Star t Time : 3 O O c 0 0 s s e c mi n

.SBe-tOO S cp s 15. 3 mi n En dTime : 5.4*5 ' 5.2e5
- 5,0*5
- 4,8*5
- 4.6e5
- 4.4*5
- 4.2*5
- 4.0*5
- 3.8*5
- 3.6*5
- 3.4*5
- 3.2*5
- 3.0*5
- 2.8e5
- 2.6*5
- 2.4*5
- 2.2*5
- Z0e5
- 1.8* 5 1.6*5
- 1,4* 5 1.2*5
- 1.0*5
- 8.0* 4 6.0*4
- 4.0*4
- 2.0*4
- 0. 0 1 3 5 14. 0 15. 0 15 5 16. 0 16 5 17. 0 17 5 16. 0 

# I SampteNam*:T(110720a138 " Sampl e ID:'GLPUMI-02- 1 &059 rFUe : "k110720a.wtt r Pee k Nama : 'PFOS ' M8ts(e9) : *499J)0Q/99.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da Commen t "DA LGW134S 0 110628 rAnnotation: - Sampl eIndex : 13 8

1.0 0

: RT : N o Int . Type : Manua l Retentio nTime : 15. B Area : 618007 8 co i Height : 4.40e-t00 5 Star t Time : 15. 3 En dTime : 16. 3 4.4a 5 4,2* 5 4,0* 5 3.6*5
- 3.6* 5 3.4* 5 3.2* 5 3,0* 5 25* 5 2.6*5
- ^ 2.4*5
- ^ 2.2*5
- I2.0*5
- 15*5
- 1.6*5
- 1.4*5
- 1.2*5
- 1.0*5
- 8.0*4
- 6.0*4
- 4.0*4
- Z0*4
- 0.0
- Sampl eType : Unkn o N/A Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePerce n Base . Sub . W Peak-Split . Repor t Larg e Mln . Pea k

He a g e 13. 3

7/22/201 1 9:14:2 5 A

H Ye s Specif y P a 5 0 ndow : 1.0 0 -act c g h t Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ e Int . Type : Retentio nT i Area : Height : Star t Time : En dTime : RT : r : 2 0.0 0 0.0 0 0 30. 0 3 15. 9 n No Manua l 15. 7 r

t 927706 0 cou r .77e-t00 5 c 15. 3 n 16. 3 r

r 6.5*5
- 6.0*5
- 5.5*5
- 5.0e5
- 4.5e5
- 4.0e5
- 3,5e5
- 3.0e5
- 2.5e5
- 2.0e5
- 1.5e5
- 1.0e5
-

15. 0 15. 5 16. 0 16. 5 17. 0 17 5 18. 0 14. 0 14 5 15. 0 15 5 16. 0 16 5 17. 0 17 5 18. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 2 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e2 4 o f 2 9 

### Pag e 7 7 O f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS
. PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b

Modified : Proc . Algor i Nois ePercentage : Base . Sub . Windo v Peak-Split . Facto i Repor t Larges t

Pe i Kin . Pea k

Height : Min . Pea k

Width : Smoothin g Width :

•360510 6 count ! 2.71e+00 5 ep ! 15. 3 mi r 16. 3 ml t 2.6e 5 25* 5 2.4* 5 25* 5 2.2* 5 Z1* 5 2.0* 5 1.9*5
- 1.8*5
- 1.7* 5 1.6*5
- 1.5*5
- 1.4* 5 1.3*5
- 1.2* 5 1.1*5
- 1.0*5
- 9.0*4
- 8.0*4
- 7.0*4
- 6.0*4
- 5.0*4
- 4.0e4
- 3.0e4
- Z0e4
- 1.0*4
- 0. 0 13 5 14. 0 

# I S3mpleN3me:-1(110720a16 r Sampl eID:-GLP1(K)1-02-18-079
- File:'V110720a.wi r Pea k Nam* : -PFOS
- Masa(*») : -499.00099.00 0 Da,499.000/BOJM O Da.499.00(V130.00 0 Da
Comment : -DA

LGW135 R0 11062 8 (d U1:100) ' Annotation ; " Sampl eIndex : 16 6 15. 0 15 5 16. 0 16. 5 17. 0 17 5 18. 0 TTme . ml n 

# I SampleName:"k110720«15 r Sampl eID:'GLP1W)1^-18-074
- Fil e Pea k Name : "PFOS
- Maes{e9) : -499.000/99.00 0 Da.499.000ffi0.00 0 Da , Comment*DALGWl%L0110628(dll:10) ' Annotatton: " Sampl eIndex : 15 7 Sampl eType ; Unknow n N/ A late dCone : 55. 5 ng/m L

7/22/201 1 3:53:0 3 P

M Modified : Proc . Algor i Nois ePe r Base . Su b w Peak-Split . Repor t L a Min . Pea k Mln . Pea k Smoothin g R TWindo w Expecte d Us eRela t
Area : Height : Star t Ti m En dTime : rg e Lhm :

^ae t S t P Heigh t Width : Width : T : v e T l KT : Y e

Spec :
sak :
3 0 . 1 5 No M an u 1 5 395162 4 .26e H 1 5 1 6 s E y P a 5 0 1.0 0 4 Ye s 0.0 0 0.0 0 0 

## )

. 9

a l

. 6 co u )0 5

. 3

. 0 4.2e S 4,0e 5 3.8e 5 3.6e 5 3.4* 5 3.2*5
- 3.0e5
- Z8*5
- 2.6*5
- 2.4*5
- 2.2e5
- 2.0e5
- 1.8e5
- 1.6e5
- 1.4eS
1.265
- 1.0e5
- 8.0e4
- 6.0e4
- 4,0e4
- Z0e4
- 0.0
- 15. 0 15, 5 16. 0 16 5 17. 0 17 5 18. 0 

# I Sampl eName : *V110720a1> r Sampl eID:-GLP1CM)1-02-lK)a6 * Faa:tcll0720a.wtt r Pea k Name : -PFOS " Masa(es} : *499.000/99.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da ' Commen t

-DA LG W136 S 0 11063 0 (d U1:10) ' Annotatkin : " Sanpl eType :

ion : Calculate dCone : Acq . Dat e Acq . Tim e Modified : Proc . Al g Nois ePe r Base . Su b rlthm :

Windo w Unknow n

N/ A 1020 . n 7/22/201 1 7:01:5 6 Ye s Specif y 5 0 P M

: 1.0 0 Peak-Split . Factor : 7 Repor t Larges t

P e Min . Pea k Min . Pea k Smoothin g R TWindo w Expecte d Us eRela t Int . Typ e Retentio n Area : Height : Star t Ti m En dTime : Heigh t

Width : Width : T : v eRT : Time : 71 i 

## ( ak : Y

e 0 0 0 30. 0 15. 9 N o Bas eT o 15. 7 151 3 c .BOetOO S 15. 3 16. 4 5 0 0 0 0 s e m i Bas e

m i m i Calculate dCon e Acq . Date : Acq . Time : Modified : Proc . Algorith m 115 . 7/22/201 1 11:35:0 1 Ye s Specif y P Nois ePercentage : 5 0 Base . Sub . Win d Peak-Split . Fa c w : l.O C

o r : 2 Repor t Larges t

Peak : Ye s Min . Pea k

Heig h Mln . pea k

Widt h Smoothin g Widt h R TWindow : Expecte dRT : Us eRelativ eR

T Int . Type : Retentio nTime : ng/m L P M aramet 6 ml n

: 0.0 0 cp s

0.0 0 se c 30. 0 15. 9 No Manua l 15. 6 Area : 809561 4 c o Height : Star t Time : En dTime : 6.40et-00 S 15. 4 16. 4 p o

ml n

c p s ml n ml n

15. 0 15 5 16. 0 16 5 17j ) 17 5 18. 0 1 3 5 14J 1 14 5 15. 0 15. 5 16J ) 16 5 17. 0 17 5 18. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 3 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e2 5 o f 2 9 

### Pag e 7 8 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : Icll0720a.rd b

IPea hName : -PFOS
- Ma3a(es) : -499.000/99.00 0 Da,499.000/80.00 0 Da,499.00CV130.00 0 Da ' • Comment:'OALGW136L0110630(dill:10)
- Annotatkjn:
- Sampl eIndex : 17 0 Sampl eType : Unknow n158 2 Concentration ; N/ A Calculate dCone : 72. 4 ng/m L42e 5 Acq . Date : 7/22/201 1 Acq . Time : 8:25:5 7 P M 4.0e 5 Modified : Ye s 3^ 5 Proc . Algorithm : Specif y Parameter s - M Q II I Nois ePercentage : SO 3.6« 5 Base . Sub . Window : 1.0 0 mi n Peak-split . Factor : 4 3.4«j 5 Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s 3.2e S Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 30e 5 R TWindow : 30. 0 se c Expecte dRT : 15. 9 mi n25e 5 Us eRelativ eRT : N o 2.6* 5 Int . Type : Manua l Retentio nTime : 15. 8 ml n- 2.4* 5 Area : 512933 3 count s g " Height : 4.33 e+ 00 5 cp s > ; 2.2e 5 Star t Time : 15. 4 ml n5 En dTime : 16. 2 ml n» 2.0* 5 1.8* 5 1.6* 5 1.4 ^ 1.2* 5 1.0e 5 6.0e 4 6.0e 4 4.0* 4 2.0* 4 15.67.S .



# 1 1 1 15.5 1 J 

# 1
1 3 5 14. 0 14 5 15. 0 15 5 

# i 16. 0 16 5 17. 0 17 5 18. 0 |Sampl*Name:"kll0720a193
- Sampl eID:"GLPHWI-02- 1 W)98 " File:1<110720a.wlf r " IPea k Name : "PFOS " Mass(es) : -499.00CI«9.00 0 Da,499.000/80.00 0 Da,499.00Q/ l 30.00 0 Da " • Comment:'DALGWl37L0110624
- Annotatfen:" " Sampl eIndex : 19 3 Sampl eType : Unknow n, 5 8 2 Concentration : K/ A-105 ^ Calculate dCone : 1.4 3 ng/m L Acq . Date : 7/23/201 1 1 OOeS Acq . Time : 4:23:5 7 A M "^ ^ M.di,l.d , V „ »•=< " Proc . Algorithm : Specif y Parameter s - M Q II Io no- j Nois ePercentage : 5 0 *'•'" " Base . Sub . Window : 1.0 0 mi nawi- j Peak-Split . Factor : 2 8.t)0e 4 Repor t Larges t

Peak : Ye s anA ^ Min . Pea k

Height : 0.0 0 ep s o.wfl w Min . Pea k

Width : 0.0 0 se c , e n. 1 Smoothin g Width : 0 point s 7.50* 4 R TWindow : 30. 0 se c Expecte dRT : 15. 9 ml n7.00* 4 Us eRelativ eRT : N o 6.50* 4 Int . Type : Manua l Retentio nTime : 15. 8 mi n„ 6.00* 4 Area : 102503 1 count s § • Height : l.O6e-f0O 5 cp s ^ 5.50* 4 Star t Time : 15. 4 mi nf En dTime : 16. 0 mi n| 5.00* 4 4.50*4
- 400*4
- 3.50*4
- 3.0064
- 250e4
- 200*4
- 1.50* 4 1.00*4
- 5000.00
- 15,66-, ^



# 1 1 

# 1 1 

# J
1 3 5 14. 0 14 5 15. 0 15 5 1 ... . Tm 

# \. , ,, , 16. 0 16 5 17. 0 17 5 16. 0 

## ^^ m= ^ ! • Sampl6Nam*:'k110720a184
- Sampl*ID:'GLP10-01~02-16-093
- Flle:'k110720a.wt r IPea k Name : "PFOS
- Ma*s(es):'499.000/99.00 0 Da.499.000/80.00 0 Da.499.000/130.00 0 Da " • Comment-DALGW137S0110624
- Annotation: ' Sampl eIndex : 18 4 Sampl eType : Unknow n Conoentration : N/ A Calculate dCone : 0.33 0 ng/m L Acq . Pate : 7/23/201 1 ^'^ ^ Acq . Time : 1:20:0 3 A M j.,^ ^ Modified : Ye s 21e 4 Proc . Algorithm : Specif y Parameter s - M O ii i Mois ePercentage : 5 0 2.0e 4 Base . Sub . Window : 1.0 0 mi n Peak-Split . Factor : 2 1.9e 4 Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s ''•°* ^ Mln . Pea k

Width : 0.0 0 se c ,_ . Smoothin g Width : 0 point s '•' " R TWindow : 30. 0 se c ,5, 4 Expecte dRT : 15. 9 mi n'"'^ ^ Us eRelativ eRT : No 1.5e 4 Int . Type : Manua l 1.4e 4 Retentio nTime : 15. B mi n- Area : 23540 7 count s & 1-3« 4 Height : 2.40et00 4 ep s ^ , _ , Star t Time : 15. 4 mi n5 ^-^ ^ En dTime : 16. 1 mi n| ,,^ 4 1.0e 4 9000. 0 6000. 0 7000. 0 6000. 0 5000. 0 4000. 0 3000. 0 2000. 0 1000. 0 15.8 2

1557. „ 

# 1 1 

# • • 

# • iJ^I'^- ' .,4k**W*v ^ >k. . 1 3 5 14. 0 14 5 15. 0 15 5 16. 0 16 5 17. 0 17. 5 18. 0 | 1 SampteName:-k110>26a198
- Sampl eID : "GLP1Wl1.02-18-10 y File:-kll0720a.wi r IPea k Name : "PFOS " Mas»(ea):'499.000/99.00 0 Da,499.000/80.000Da.499.000fl30.00 0 Da " • Comment'DALGW138R0110630{dil1:10) " Annotatton:
- Sampl eIndex : 1S 8 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 348 . na/m L2.1*6
- Acq . Date : 7/23/201 1 Acq . Time : 6:13:5 8 A M 2.0*6
- Modified : Ye s 1.9*6
- Proc . Algorithm : Specif y Parameter s - M QII I Nois ePercentage : 5 0 1.8*6
- Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 2 1.7* 6 Mln . Pea k

Height : 0.0 0 cp s 1,6*6
- Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 1,5* 6 R TWindow : 30. 0 se e Expecte dRT : 15. 9 mi n1.4* 6 Us eRelativ eRT : No 1.3* 6 Int . Type : Manua l Retentio nTime : 15. B mi n„ 1^ 6 Area : 2300666 3 count s g " Height : 2.15et00 6 ep s > ; 1.1e 6 Star t Time : 15. 3 ml n» En dTime : 16. 6 ml n| l.0e 6 9.0e 5 8.0* 5 7.0* 5 6.0e 5 5.0e 5 4.0e 5 3.0e 5 2.0e5
- 1.0e5
- Thn em h

1 5 8 1 1

15.66, . 

# 1 1 

# 1 

# • • • 

# • m . 1 3 5 14. 0 14 5 15. 0 15 5 16. 0 16 5 17. 0 17 5 18. 0 | 1 

### Dat aprinte db y ST

W Printin g Time : 12:06:0 3 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e2 6 o f 29 Pag e 7 9 O f11 3GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 •ETS-Kir k Result s Name : kll0720a.rd b

l i Sampl*Nam*:-k110720a207 ' Sampl e ID : "GLP(frO1-02-16-10

r Fil*:-k110720a.vKt

r IPaa k Nam* : 'PFOS
- Maas(es> : -499JXXV99.00 0 Da,499X00/80.00

0 Da,499,000/130.00

0 Da " • Comm*nt:-DALGW138L011063a
- /Annotatton:
- Sample Index : 207 Sample Type : Unknow n Concentration : N/ A Calculate dCone : 0.33 9 ng/m L1.9* 4 Acq . Date : 7/23/201 1 Acq , Time : 9:22:5 4 A M ,_g ^ Modified : Ye s Proc . Algorithm : Specif y Parameter s - M

Q II I'•'* * 1 Nois e Percentage : 5 0 Sase . Sub . Window : 1.0 0 min1.6* 4 Peak-Split . Factor : 4 Repor t Larges t

Peak : Ye s 1.5*4 Min . Pea

k Height : 0.0 0 cp s Min . Pea

k Width : 0.0 0 se c i4« 4 1 Smoothin g Width : 0 point s R TWindow : 30. 0 se c ,- . Expecte dRT : 15. 9 ml n'-* ^ Us e Relativ e RT : N o 1.2* 4 Int . Type : Bas e T o Bas e Retentio nTime : 15. 8 mi n« 1.1* 4 Area : 24159 7 count s & Height : 1.97e + 00 4 cp s > L10* 4 Star t Time : 15. 4 ml n1 En dTime : 16. 2 ml n| ^QQQQ 8000. 0 7000. 0 6000. 0 5000. 0 4000. 0 3000. 0 2000. 0 1 1000. 0 ...M.MUL,,M.L.,,,iMl<^ ^ 13 5 14. 0 14 5 15. 0 1 Sampl e ^Jame:4<110720a22

r Sampt e ID:'-GI>1W)1^18-118- ' t = Be : i<110^20*.«* r 1 Pea k Name : "PFOS
- Mass(*») : "499.OOW99.00 0 Da.499.000BO.00

0 Da.499.000/130.00

0 Da " • Comment:-DALGWCWi6C0110622(dll1:10)
- AnnotaUon:
- Sample Index : 22 1 1 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : 398 . ng/m L36* 5 Acq . Date : 7/23/201 1 Acq . Time : 2:16:5 2 P M g^ g Modified : Ye s Proc . Algorithm : Specif y Parameter s - M

Q II I3.2e5 Noise Percentage : 5 0 Base . Sub . Window : 1.0 0 mln3_0e 5 Peak-Spilt . Factor : 6 Repor t Larges t

Peak : Yes Min . Pea

k Height : 0.0 0 cp s ^-" ^ Min . Pea

k Width : 0.0 0 se c Smoothin g Width : 0 polnl a2.6* 5 R TWindow : 30. 0 se c Expecte dRT : 15. 9 min2_4« 5 Us e Relativ e RT : N o int . Type : Bas e T o Bas e ^,2* 5 Retentio nTime : 16. B ml n« Area : 563028 7 count s & 2.0*5
- Height : 3.74e*0O 5 cp s ^ Star t Time : 15. 4 minS ^ g^ . En dTime : 16. 4 ming

~ 1.6*5
- 1.4*5
- 1.2*5
- 1.0*5
- 8.0*4
- 6.0* 4 4.0*4
- 2.0*4
- 13 5 14. 0 14 5 15. 0 155 3 1

15.69., .


# 1 

# 1 

# • • • 

# 1 • i5.5a.^^ i 

# J L
15 5 16. 0 Tlme.mi n

157 9 15.te, ^

H ^ H ^ H ^ H 

# • • • • • m 

# J L
15 5 16, 0 17.10, , 17.3 1

1«. 5 17,0 17 5 18. 0

16. 5 17. 0 17 5 18. 0 IPea k Name : "PFOS " Man<*B} : '499.000/99.000 Da,4a9.000/S0.00 0 Da,499.000/130.00

0 Da " • Conr«i*nt-DALGW138S0110630((fil1:10) " Annotatkm;
- Sampl e Index : 21 2 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : 194 . ng/m L1 1Se6 Acq . Date : 7/23/201 1 Acq . Time : 11:08:0 2 A M 1,10* 6 Modified : Ye al.OSeS Proc . Algorithm : Specify Parameter s - M Q II I Nois e Percentage : 5 0 1.00* 6 Base . Sub . Window : 1.0 0 minociv e Peak-Split . Factor : 2 9.50e & Repor t Larges t

Peak : Ye s ' oonu l Mln . Pea

k Height : 0.0 0 cp s w.uue o Mln . Pea

hWidth : 0.0 0 se c 850* 5 Smoothin g Width : 0 point s R TWindow : 30. 0 ae c 8.00* 5 Expecte dRT: 15. 9 ml n Us e Relativ e RT : N o 750e 5 Int . Type : Manua l ' 7.00* 5 Retentio nTime : IS. 8 min» «KA- C Atea : 1335905 0 count s g " ^-'** ° Height : 1.19e*00 6 cp s > ; ar«« ^ Star t Time : 15. 3 ml nf

**-"^ ^ En dTime : 16. 3 ming 5.50* 5 5.00* 5 4.50* 5 4.00* 5 3.50* 5 3.00* 5 2.50e5 2.00* 5 150* 5 1.00e5 5.00e 4 15.8 1 1

15.67-^ 1 

# 1 1 1 

# • • 

# 1 • 

# • 

# M^
13. 5 14. 0 14 5 15. 0 15. 5 16. 0 16 5 17. 0 17 5 18. 0 1 Thne.m h1 |Sampl*Nam*:'k1l0^26a226 ' Sampl e ID:-Gi>1(M]1-02-18-12 r File:-k110720a.wi

r IPea k Name : "PFOS " Mass(e«) : •499.000«9.000 Da.499.000«0.000 DB.499.000/ 1 30,000 Da " • Commen

t "DA LG WCW26 L011062 2 (d

Q1:10)
- Annotatkin:" " Sanpl e Index : 22 6 Sanpl e Type : Unknow n Concentration : N/ A95e 5 Calculate dCone : 911 . no/m L Acq . Date ; 7/23/201 1 Acq . Time : 4:01:3 9 P M a.ueo- ' Modified : Ye s 8.5e5 Proc . Algorithm : Specify Parameter s - M Q II I Nois e Percentage : 5 0 B.Oe S Base . Sub . Window : 1.0 0 mln Peak-Split . Factor : 6 y g^g . Repor t Larges t

Peak : Yes Mln . P*a

k Height : 0.0 0 cp s Mln . Pea

k Width : 0.0 0 se c '•''*' ' Smoothin g Width : 0 point s R TWindow : 30. 0 se c 6.5e 5 Expecte dRT : 15. 9 ml n Use Relativ e RT : N o 6.0* 5 Int . Type : Bas e To Bas e S5» S Retentio nTime : 15. 8 ml n« " Area : 1257391 1 count s & ^ „ ^ Height : 9.58 e + 00 5 cp s > ; '•"* • Star t Time : 15. 4 min1 En dTime : 16. 4 ml ng 4.Se5
-



## - 4.0* 5 3.5* 5 3.0* 5 2.5* 5 2.0e S 1.5* 5 1.0e 5 5.0e4
- 15.8 1

15.65, .



## . ,

y 

## • H 

# • • 1 1 I M 

# m ^

13 5 14. 0 14 5 15. 0 15 5 16,0 16 5 1^. 0 17 5 18. 0 | | ^*""" ' = u Dat aprinte db y ST

W Printin g Time : 12:06:0 3 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 7 o f 2 9 Pag e 8 0 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0720a.rd b 

# T Sarrvl * Nanw : l(110720a237 - Sampl e (D : "C^P I 04)1-02-18-128- File : "k l 10720B.Mi r P*a k Nam* : 'PFOS ' Ma8s(*s) : "499.00099.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da " Comment:-DALGW140R0110622{dU1:10) - Annotatton:" " Sampl e Index : 23 7 Sampl e Type : Unknow n 

# I T

Acq . Dat e Acq . Tim e Modified ; Proc . Al g Nois ePe r Base . Su b Peak-Spl i Repor t L a Min . Pea k Min . Pea k Smoothin g R TWindo w Expecte d Us eRela t Int . Typ e Retentio n Area : Height : Star t Ti m En dTime : rlthm :

entag e 7/23/201 1

7:52:1 6 P

M Ye s Specif y Par a SO Window : 1.0 0

. Fact e r : 2 ges t Peak : Ye s Heigh t Width : Width :

v eRT : Time : 0.0 0

0.0 0 0 30. 0 s e 15. 9 m i N o Manua l 15. 7 m i 832564 7 coun t 5.91e+00 5 c p 15. 3 m i 16. 4 m i

15, 0 15, 5 16. 0 16. 5 

# I Sanvil*N3m*:-fcl10720a25 rSampl e ID:-GLPl(M)1-02-18-14a ' FSe: ' Pea k Name : "PFOS " Ma»(*s) : "499.000il99.00 0 D a ,499.000/80,00 0 Da.4 9 Comment : "DA LG W142 R 011062 2 (d U 1:10} - Annotatkin : " " Sampl eIndex : 25 1

^ : Unknow n 5.2* 5 5.0* 5 4 5 ^ 4.6* 5 M Q II I 4.4* 5

4.2* 5 4.0* 5 35e 5 3.6 ^ 3.4* 5 3.2* 5 3.0* 5 & 25* 5 f 2.6* 5 2.2* 5 2.0* 5 15* 5 1.6* 5 1.4*5
- 1.2*5
- 1.0*5
- ei)*4
- 6J>*4 ' 4.0*4
- 2.0*4
- S8mpl*Nam«:-1(110720a246 ' Sampl * ID:-GLP1(M)1-02 - P*a k Nam* : 'PFOS - Masst*a) : "499.000/99.00 0 Da,499. ~ Camm*nt-OALGW141R0110630 - Annotation: ' Sampl eIndex : 24 6 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 3.0 4 ng/m L Acq . Pate : 7/23/201 1 Acq . Time : 11:01:1 7 P M Nol i Per

t Ye s

SO Base . Sub . Window : l. ( Peak-Split . Factor : 2 Repor t Larges t

Peak : Y

< Mln . Pea k

Height : 0 . Mln . Pea k

Width : 0 , Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 15. 9 Us eRelativ eRT : No , t . Type : Height : Star t Ti n En d Time ; Ma m

2.18e+00 5 cp s •18-135 " Fil*: 1 .000/80.00 0 Da,4 !

2.1* 5 2.0* 5 1.9* 5 1.8* 5 1.7* 5 1.6* 5 1.5* 5 1.4* 5 1.3* 5 1.2*5
- l.leS ' 1.0* 5 9.0*4
- 8.0*4
- 7.0*4
- 6.0*4
- 5.0*4
- 4.0*4
- 3.0*4
- 2.0*4
- 1.0*4
- 0. 0 15. 0 15. 5 16. 0 16 5 17. 0 17. 5 18. 0

Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 82. 6 Acq . Date : 7/24/20 1 Acq . Time : 12:46:2 9 Modified : Ye s Proc . Algorithm : Specif y Nois ePercentage : 5 0 

## . A M

Base . Sub . Window : 1.0 0 m Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0 . Mln . Pea k

Width : 0 . Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 15. 9 Us eRelativ eRT : N o Int . Type : Manua l Retentio nTime : 15. 8 Area : 583873 8 e Height : S.27e+00 5 Star t Time : 15. 4 En dTime : 16. 2 ) 0 1 0 s e e mi n

c p s ml n ml n 

# I SampteName:'l(110720a26a ' Sampl e ID : "GLP I (Mi l •02-18-145 - File:* ! Pea k Nam* : TFOS " Mass(es) : -499.000/99.00 0 Da,499.000/80.00 0 Da,4 9 Comnwn t -DA LG W143 R 011062 2 (d B 1:10) - Annotation : " Sampl eIndex : 26 0 Sampl eType ;

0. 0 N/ A Calculate dCone : Acq . Date : Acq . Tims : Modified : Pcoc . Algorithm : Mols ePercentag e Base . Sub . Windo v Peak-Split . Fact e 17. 8 7/24/2 0 3:55:3 8 Ye s Specif y ng /

1 A M Param e : 1.0 0 m l r : 6 Repor t Larges t

Peak : Y Mln . Pea k

Heigh t Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Area : 12 ' Height : Star t Time : 0 0 0 30. 0 15. 9 No Manua l 15. 7 455 0 c .82e+0 0 is. 4 0 0 e 0 0 a a « e ml n
ml n ep H ml n

15. 0 15 5 16, 0 16. 5

Tlme.mi n 9.5e 4 9.0*4
- 8.5*4
- 6.0*4
- 7.5*4
- 7.0*4
- 6.5e4 : 6.0*4 ^ 5.5*4 : 5.0*4
- 4.5*4 ; 4.0*4 : 3.5*4 : 3.0*4 ; Z5*4
- 2.0*4 : t.5*4
- 1.0*4 ' 5000.0 ' 0. 0 15. 0 1S. 5 18, 0 16 5 17. 0 17 5 18. 0 Dat aprinte db y ST

W Printin g Time : 12:06:0 3 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 8 o f 2 9 201 1 

### Pag e 8 1 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0720a.rd b 

# r S«m;fcName:1<110720a265 - Sampl e ID:-GLP1(H)1-02-1 & Pea k Nanw : -PFOS * Mass(as) : -499.000/99.00 0 Da,499.000/ Comnient:"OALGW1**S0110628(dai:10) - AiwotaUon: " Sampl eIr.dex : 26 5 Sampl eType : Unknow n Concentration : N/ A Calculate dCone : 8,6 9 Acq . Date : 7/24/201 1 Acq . Time : 5:40:4 8 A M Modified : Yes Proc . Algorithm : Specify Par Base . Sub . Window : 1.0 0 Repor t Larges t Peak : Yes Hin . Peak

Height : 0.0 0 Mln . Pea k Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30. 0 3 Expecte dRT: 15. 9 m Us eRelativ eRT : N o Int . Type : Bas eT o Ba ; Retentio nTime : 15. 7 m Area : 62301 2 coun ng/m L

ameter s mi n e p s pol r I n Height : 4.£5e-t00 4 cp s En dTime : 16. 2 m i n - M Q II I

t s 

## & i 4.4* 4 4,2* 4

3.4* 4

2.8* 4 2.4* 4 2.2* 4 2.0* 4

1.4e 4 1.2* 4 1.0e 4 8000. 0 6000. 0 400D.O ' 2000. 0 0. 0 •i^-vM^ttiefj^Maito p 

# I SamptoNam*:-kl10720a145 " Sampl * 10:-GLP1&O1-02-1&O66 ' FUe: 1 Pea k Nam* : 'PFOS - Mass(*s) : '499.000/99.00 0 Da,499.000/80.00 0 0a,4 ' Comman t -Tri p Blan k

S* t1 MS - Annotation : ~
9.5* 5 9.0* 5 8.5* 5 8.0* 5 7.5* 5 7.0* 5 6.5*5
- 6.0*5
- 5.5*5
- 5.0*5
- 4.5* 5 4.0* 5 3.5*5 : 3.0*5 : 2.5*5 : 2.0e5 : 1.5*5
- 1.0*5 : 5.0*4 : Sampl eType : Concentrati o Calculate dC Acq . Pate : Acq . Time : Modified : Proc . Algor i Nois ePerce r thm :

-aq e Base . Sub
- Wind

o Peak-Split . Min . Pea k H e Mln . Pea k 'H i Smoothin g W

i R TWindow : Expecte dRT : Us eRelativ e Int . Type : Retentio nTi Height : Unknow n N/ A 9.4 5 7/22/201 1 11:41:1 5 A

M N o Specify Par 5 0 : 1.0 0 factor : 2 S t Peak : Ye s iqh t dth : dth : RT : 0.0 0

0.0 0 0 30. 0 s 15. 9 m N o Valle y 15. 8 m 666165 1 coun .OOe+006 c

p

0. 0 13. S 14. 0 14. S 15. 0 15. 5 16. 0 16. 5 17. 0 17. 5 18. 0

Dat aprinte db y ST

W Printin g Time : 12:06:0 3 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e2 9 o f 2 9 

### Pag e 8 2 O f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729b.rdb

( 0 0 ) 

## < w

• — 

### m 

## g > < l U C O 

### c 

## < 5 5 5 0 4 5 4 0 3 5 3 0 2 5

4 5 5 0 5 5 6 0 Analyt e Cone
. /

I S Cone
. Dat a printe d b

ySTW Printin g Time : 12:08:2 2 PM Printin g Date : Tuesday , Septembe r 06 , Pag e1 o f 1 201 1 

### Pag e 8 3 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0729b.rd b

•S«mpl*Nam*:T(110729b0i rSamf* * ID : "1 1 005-25 ^ Fl* : •k110729b.wt r I Paa k Nam*:-13CSPF0S^S(IS) - Man(M):''507.0001/80.00 0 Da ' I Comnwnt : "2. 5 ng/m LF C et d I n Synth
. Wate rAnnotation : ~ Sampl e Index : 1 7 Sampl e Type : standar d Concentration : 0.96 0 ng/m L Calculate d Cone : N/ A Acq . Date : 7/30/201 1 Acq . Time : 10:52:4 0 P H Modified : Ye s Proc . Algorithm : intelllQua n - IQ AI I Mln . Pea k Height : 0.0 0 cp s Mln . Pea k Width : 0.0 0 se c Smoothin g Width : 0 point s B T Window : 30. 0 se c Expecte d RT : 16. 2 ml n Us e Relativ e RT : M o Int . Type : Valle y Retentio n Time : 16. 1 ml n Area : 69711 5 count s Height : l.lSe'OO S cp s Star t Time : 16. 0 ml n En d Time : 16. AnI n 

## i 1.15* 5 1.10* 5 1.05* 5 1.00* 5
9.00* 4 8.50* 4 8.00* 4 7,50* 4 7.00* 4 6.50* 4 6.00* 4 5.50* 4 5.00* 4 4.50* 4 4.00* 4 3.50* 4 3.00*4
- 2.50* 4 2.00* 4 1.50* 4 1.00* 4 5000.0 0 0.00 ' 

# I Sampt*l4am*:'k110729b028 ' Sampl e ID:'LCS-110726-1 ' Fie:'V110729b.w( r P«>kNam*:'13C8PFOS-tS(IS rMss3<«a) : "SOT.OOCVBO.OO O Da ' Comm*nt : t)J2pp b LCS " Annotation : ~ Sampl eIndex : 2 8 Sampl eType : O C Concentration : 0.95 3 ng/m L Calculate dCone ; N/ A Acq . Date : 7/31/201 1 Acq . Time : 2:44:3 3 A M • Modified : H o Sampt*Nam*:-1(110729b02 rSampt * ID:'1 1 002-243 ' FO*:-kl10729b.wt r P*a k Nam* : -13C8PFOS-IS(IS) - Ma«3<es) : "507.000/80.00 0 Da Commen t *M*tho d Blani rAnnotatkin : SampJ ' Sampl eType : ng/m L Calculate d Cone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : N/ A 7/31/201 : 2:23:3 0 A M IntelllQ u : 0.0 0 0.0 0 0 30. 0 16. 2 a n - IQ AI I

cp s ae c point s ml n 1.2* 5 Us eBelatlv . valle y 16. 1 0 3 33 1 c c 1.50e+00 5 16. 0 16. 3 ml n

cp n ml n ml n 

## I ^ 

# 1 1.0* 5 9.0* 4 8.0* 4 7.0* 4

14. 0 14. 5

Proc . Algorithm : Hln . Pea k Height : Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : ntelll Q 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 2 Area : 57307 1 c c Height : 9 Star t Time : End Time : 17et00 * 16. 0 16. 1 a n -

se c ml n
ml n ml n ml n Modified : Proc . Algorithm : Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : N o IntelllQ. ! 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 2 Area : 62919 0 C O Height : Star t Time : .07e+00 5 16- 1 

# I SampteNam*:1<110729b03 rSampl*ID:-LCS-110726-4 ' FI*:'V110729b,\»W r P*akNam*:'13C8PFOS-IS{IS) ' Uas9(*a) : ^5O7.00(V80.00 0 Da ' Commen t *2pp b LCS " Annotation: " Sampl e Index : 3 1 Sampl e Type ; Q C Concentration : 0.95 3 ng/m L Calculate d Cone : N/A1.05*5 ' Acq . Date : 7/31/201 1 Acq . Time : 3:47:5 5 A H 1.00*5 ' 9.50*4 '
8.50*4 - 8.00e4 - 7.50*4 - 7.00*4 - 6.50*4 - « 6.00*4
- ^ 5.50e4
- g 5.00*4
- 4.50*4
- 4.00*4
- 3.50*4
- 3.00*4
- 2.50*4
- 2.00*4
- 1.50*4 - 1.00*4 ' 5000.0 0 0.0 0 

# .J^r^, . Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 o f 7 

### Pag e 8 4 O f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729b.rd b

•SampleNam*:1(110729b034 - Sampl e ID:'LCS-11072&- rFl*:'k110729b. w •Paa k Name:'13C8PFOS-IS05} ' Mass(*s):'507.000/BO.OO O Da " I Commen t'20pp b LCS " Annotatkm:' " Sampl e Index : 3 4 Sampl e Type ; Q C Concentration : 0.95 3 ng/m L CalcuUte d Cone : N/ A Acq . Date : 7/31/201 1 14 ^ Acq . Tine : 4:51:1 7 AM 1.3* 5 Modified : Proc . Algorithm : Min . Pea k Heigh t Min . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Ye s IntelllQua n : 0.00 . c 0.0 0 3 0 p o 30. 0 s 16. 2 m N o Valle y 16. 2 m Area : 90096 1 cou n Height : Star t Time : L.48et00 5 c 16. 0 m 8.0*4 7.0* 4 6.0* 4 5.0* 4 4.0*4 ' 3.0* 4 Z0e 4 1.0*4
- 0.0 - 14. 0 14. 5 ../TVlH. . 

# I Samp4*Nam*:Tc110729bO44 ' Samp* * tD:'LCS-110726-13 ^ File:'k110729b.w4 r P*akName:'13C8PFOS^S05) - Mass<*s):'5O7XO0/BO.OO 0 Da ' Comm*nt : "2pp b LC S ECF " Annotatton; " Sample Index : 4 4 Sampl e Type : Q C Concentration : 0.954 ng/m L Calculate dCone : N/ A Acq . Date : 7/31/201 1 Acq . Time : 8:22:2 5 A M Sampl * Nam* ; "k110729b04 rSampl e ID:'LCS-110726-10 ' FJl*:-k110729b. w P*a k Nam* : '13C8PFOS-IS(IS) ' Ma«s<Bs) : -S07JX)0/80.00 0 Da ' Commen t •0.2pp b LC S EC P Annotatfen: " Sampl e Index : 4 1 Sampl e Type : Q C Concentration : 0,95 3 ng/m L_^ Calculate d Cone : N/AS-5«4 - Acq , Date : 7/31/201 1 Acq . Time : 7:19:0 5 A M 9.0*4
- 8.5*4 - AI I 8.0*4 -

7.5*4
- 7.0*4
- 6.5*4
- 6.0*4
-

- 5.6e4
- S . 

### i '•°" - I4.5*4
- 4.0*4
- 3.5*4
- 3.0*4
- Modified : Proc . Algorithm : Mln . P-a k Height : Mln . Paa k Width : Smoothin g Width : R T Window ; Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Ye s ntelllQ- j 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 Area : 58922 1 c o Height : 9 75e+00 4 

# I ,./s». .

Modified : Proc . Algor i Mln . Pea k H e hm ;

ght : Min . Pea k Width : Smoothin g Width ; R T Window : Expecte d RT ; Us e Relativ e Int . Type : Retentio n T l Area : Height : Star t Time : En d Time : RT : Ye s intelllQ L 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 57734 3 C O 1 OOe-tOO S 16. 0 16. 3 a n - 1

cp s point s ml n
mi n cp s mi n 

# I SBn«)leName:'V11072gb04 rSamptelD: ' P*akNam*:'13C8PFOa4S(IS> ' Mas9(*s): ' Commen t '20pp b LC S EC P Annotatkin : ~ Sampl e Index : 4 7 Sampl e Type ; Q C Concentration : 0.95 3 ng/m L Calculate dCone : N/ A Acq . Date : 7/31/201 1 Acq . Time : 9:25:4 5 A M Modified : Proc . Algorith m Mln , Pea k Heig h Mln . Pea k Widt h Smoothin g Widt h R T Window : Expecte d RT : Us e Relativ e R T Int . Type : Retentio n Time : Ye a IntelliOua n - I : 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 Area : 66635 2 c Height : Star t Time : En d Time ; 1.07et00 5 16. 0 16.4 cp s point s se c ml n

cp s ml n ml n

16J ) 16. 5 1.05* 5 l.OOeS 9.50e 4 9.00e 4 S.50e4
- 8.00*4
- 7.50* 4 7.00* 4 6.50* 4 6.00* 4 % 5.50*4
- 

### f I 5.00*4
- 4.50*4
- 4.00*4
- 3.50*4
- 3.00*4
- 2.50*4
- Z00*4
- 1.S004
- 1.00*4
- 5000.00
- 0.00
- 

## ,.A?s».>. , Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 o f 7 201 1 

### Pag e 8 5 O f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Klr k Result s Name : kll0729b.rd b 

# I SampteName:"k110729b072 ' Sampl e ID:-GLP1(K}1-02-1&093 - File:-k110729b.wir r Paa k Nama ; '•13CSPF0&^S<IS} ' Ma89(es) : '507J)OQ/80.00 0 Da * Comment:-DALGW137S0110624 - Annotatton: " Sampl e Type ; Calculate dCon e Acq . Date : Acq . Time : Modified : Proc . Algorith m Mln . Pea k

Heigh ' Min . Pea k

widt h Smoothin g Wldth
R TWindow ; Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : Unknow n 0.96 0 N/ A 7/31/201 1 6:14:2 7 P Ye s IntelliQ u : 0.0 0 0.0 0 30. 0 16. 2 No valle y Area : 73390 1 c o Height ; Star t Time : En dTime : 1.25e'(-00 5 16. 0 16. 4 1.20* 5 1,15*5 ' 1.10* 5 1.05e 5 1.00*5
- 9.50*4
- 9.00*4
- 8.50*4
- 8.00e4
- 750e4
- 7.00e 4 6.50e4
- 6.00*4
- 5.50* 4 5.00* 4 4.50* 4 4.00* 4 3.50e 4 3.00*4
- 2.50* 4 2.00* 4 1.50B 4 1.0Oe 4 5000.0 0 0.00 ' 16.1 4

14. 0 14, 5 

### • ,/VS,—,. . SampieName:1c110729b086 ' Sampl e ID:'GLP1041-02-18-108 - File : 1(110729b.wi r Pea k Nam* : -13C8PFOS-(S(IS) ' Mass{ee) : '507.000/80.00 0 Da Comment:-OALGW138L 0 110630 ' Annotatnn: - Sampl e Index : 8 6 Sampl e Type : Unknow n Conctntratlon : 0.96 0 ng/m L N/ A I SampleNam*:'k110729b08 rSampt*ID:'GLP1001-02-18-098 ' File:-1(110729b.wi r 

# I P*a k

Name : -13C8PFOS-IS0S) ' Ma«{*9) : "507.000/80.00 0 Da ' CommenfDALGW137L0110624 ' Annotatton: " Sampl e Index : 8 1 Sampl e Type ; Unknow n Concentration : 0.96 0 Calculate dCone ; N/ A Acq . Date ; 7/31/201 : ng/m L Acq . Time : 9:24:5 9 P M Modified : Ye s Proc . Algorithm ; IntelllQ ' Mln . Pea k

Height : 0.0 0 Mln . pea k

«idth : 0.0 0 Smoothin g Width : 0 R TWindow ; 30. 0 Expecte dRT : 16. 2 Us e Relativ e RT : No Int . Type : Valle y Retentio nTime : 16. 1 Area : 100465 2 c o Height : l.BletOO S Star t Time ; 16. 0 En dTime : 16. 5 cp s a e c poin t

ml n c p s mi n IQ A1 1 

## =
& 1.8*5- 1

1.7*5
- 1.6*5
-

1.4*5
- 1.3*5
- 1.2 * - 1.1*5
- 1.0*5
- 9.0*4
- 8.0*4
- 7.0*4
- 6.0*4
- 5.0*4
- 4.0e4
- 3.0*4
- Z0e4
- 1.0*4
- 

# I San ^

Nam* : "kll0729b095 * Sampl e ID : 'GLPI0^1-02-18-145 * File : 'k110729b.>vt r P*a k Name : -13C8PFOS-IS(IS} - Mass{*a) : '507.000/80.00 0 Da ' Comment'DALGW143 R 0110622-ISaddac TAnnotatton:" " Sanpl e Index : 9 5 Sampl e Type : Unknow n Concentration : O.96 0 ng/m L Calculate dCone : N/ A Acq . Date : 8/1/201 1 Acq . Time : 2:21:2 5 A M lulate dCone : Acq . Acq . Dat e Tim e Modified : Mln . Algor i Pea k Pea k Smoothin g R TW ndo w Expecte d He hm :

• gh t Width : Width ; ;T : Us e Relativ e Int . Typ e Retentio n Are a T i KT ; 7/31/201 1 11:10:4 7 Ye s IntelliQ u 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 59890 7 C

O Star t Time : 1.05*5
- LOOeS
9.50e4
- 9.00*4
- 6.50*4
- 6.00*4
- 7.50*4
- 7.00*4
- 6.50*4
- « 6.00*4
- * 5.50*4
- I5.00*4
- 4.50*4
- 4.00*4
- 3.50*4
- 3.00*4
- 2.50*4
- 2.00e4
- 1.50*4
- 1.00*4
- 5000.0 0 0.0 0 Modified : Proc . Algorith m Mln . Pea k

Heig h Mln . Pea k

Width ; Smoothin g Width ; R TWindow : Expecte dRT : Us e Relativ e R

T Int . Type : Retentio nTime : Ye s IntelII Q : 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 Area : 85259 2 c c Height ; 1.52et00 5 

# i

../Vs^^ , Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M . Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e3 o f 7 

### Pag e 8 6 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729b.rd b 

# T Sampl*NBm*:>110729t>100 ' Sampl e 10:'GLP1041-02-1B-150 ' Fll*:'k110729b.wl r Paa k Nam* : '13C8PFOS-IS0S) ' Maaa(*8): "507.000/80.00 0 Da ' Comniant:'DALGW144S0110628-ISBdd«d - Annotatkin: " Sampl eType : Calculate d Co i Acq . Date : Acq , Time :

Min . Pea t Width : Smoothin g Width : R T Window : Unkn c 0.96 0 ng/m L

itelliQua i 0.0 0 • 0.0 0 :
6 Relatlv . it . Type : Height : Star t Tlm ( End Time : l.38e+0O 4 cp s 8.0*4
- 7,5*4
- 7.0*4
- 6.5* 4 6.0* 4 5.5* 4 5.0* 4 4.5* 4 4.0* 4

2.5* 4 2.0* 4

14. 0 14. 5 

# 1 ./rs„ , 

# I Sampl * Name : l(110729b027 ' Sampl * ID:'1 1 002.243 ' Fle:1d10729b.w< r Pea k Name : 'PFOS * Massf**) ; '499.OCW99.00 0 Da,499.000/80.00 0 Da.499.0D(V130.00 0 Da Comment : "Mattto d Blank ' Annotatton : Sampl eIndex : 2

7 Sampl eType : Unknow n ng/m

L 7/31/201 1 3 0 A M 400 0 

# I SampleName:'k110729bOi rSampl*ID:'1 1 00»2&« ' Fil*:'k1l0729b.»fl r P*a k Nam* ; 'PFOS * Masa<*B) : "499.000/99.00 0 Da,499.000/80.00 0 Da,499.000/130.00 0 Da ' Commen t *2. 5 rqjAn LF C et d I n Synth
. VVM* rAnnotafion:" " Sampl eType : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentag e Standar d

2.5 0 ng/m

L 2.SAng/m

L 7/30/201 1 10:52:4 0 P

M Ye s Specify Paramet e 5 0 Base . Sub . Window : 1.0 0 mln Peak-Split . Fac t Repor t Larges t P Mln . Pea k Heigh t Mln , Pea k Width : Smoothin g Width : R TWindow : Expecte dKT: Us eRelativ eRT : int . Type ; Retentio nTime : :ak : Yes 0.0 0 cp s 0.0 0 ae c 0 p o 30. 0 se c 16. 2 ml

n No llanua l 16. 2 ml

n Atea : 198006 2 count s Height : Star t Time : En dTime : i.27e+00 5 cp s

16. 1 ml

n 16. Amln

Modified : Proc . Algorithm : S | NoisePercentage : Base . Sub . Window : Peak-Split . Facto r f yPa i

- M QII I

Pea k Height : Hln Mln . Pea k Width : Smoothin g Width : R TWindow : Expecte dRT : UseRelativ eRT : Height : Star t Tl i En dTime : 16. 2

2284 1 CO

I t.lSe-tOO S 16. 1 

# I Sampl * Name : •k110729b028 - Sampl * ID : "LCS-110726-1 - F(e:*V110729b.wi r P*a k Nam* : 'PFOS ' Mas*(fls) : '499.000/99.00 0 Da,499.000/80,00 0 Da,499.000/130.00 0 Da " Comman t '0.2pp b LCS ' Annotation: " 2.6* 4 2.5* 4 2.4*4
- 2,3*4
- 2.2*4
- 2.1*4
- 2.0*4
- 1.9*4
- 1.8*4
- 1.7*4
- 1,6*4
- 1,5*4
- Sampl eIndex : Sampl eType : Calculate dCone : Acq . Date : Acq . Time : Modified : Ptoc . Algorithm : Nois ePercentag e Base . Sub . Wind

o 2 8 Q

C 0.19 9 0.25 7 7/31/201 1 2:44:3 3 A

M N o Specify Pa

r 5 0 J : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t Peak : Yes Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : 0.0 0 0.0 0 0 30. 0 a 16. 2 m No Valle y 16. 2 r Area : 1695J 5 coun Height : Star t Time : .6<e+00 4 c

16. 1 m ng/m

L ng/m L

amet e

c p s

e c I n
L n I n 1.4*4 ' 1.3* 4 1,2* 4 1.1* 4 1.0* 4 9000. 0 aooo. 0 7000. 0 6000. 0 5000.0
- 4000.0
- 3000. 0 2000. 0 1000.0
- 0. 0 i.fi»nl«H<*i|i<lDil>*H(ii*i|li | 16. 0 16. 5 Tim* , ml n Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e4 o f 7 

### Pag e 8 7 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS
. PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729b.rd b 

# r Sampl e Nam*:"k11072aj03 rSampl e ID:'LCS-11072S-» ' Fl*:'1<110729b.wi r P*a k Nam* : 'PFOS ' htas3(*8) : '499.000/99.00 0 Da,499.000/80.00 0 Da.499.000>'130.00 0 Da ' Comnten t'2pp b LCS ' /knnotatton: " Sampl eIndex : 3 1 Sampl eType : Q C Concentration : 1. 9 9 Calculate dCone : 2.1 1 Acq . Date : 7/31/201 1 Acq . Time : 3:47:5 5 A M Modified : N o Proc . Algorithm : Specify Par Nois ePercentage : 5 0 Base . Sub . Window : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t Pe«k : Ye s Min . Pea k Height : 0.0 0 Min . Pea k Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30. 0 3 Expecte dRT: 16. 2 m Us eRelativ eRT : N o Int . Type : Valle y Retentio nTime : 16. 2 m Area : 149474 8 coun ng/m L ng/m L

amete ml n

s Height : 2.53etO0 5 cp s Star t Time : 16. 1 m En dTime : 16. 4 mln

16. 5 17. 0 18. 0 16. 5 Sampl*Nam*:"k110729b041 ' Sampl * ID : •LCS-110726-10 " File:'"1(110729b.yiiifr ' P*a k Name : "PFOS ' Maa3(*9) : '499.000/99.00 0 Da,499.O0O/SD.O00 Da,499.000/130.00 0 Da Comment : "0.2pp 6 LC S ECF " Arewtatton: - Sampl eInd i 

# I Sampl * Nam* : 1(1107291)034 - Sampl e ID:'LCS-110726-7 " Fta:-k110729l].«tf r P*a k Nam* : "PFOS " Mseatee) : '499.000/99.00 0 Da,499.000/80.00 0 Da.499.000/130.00 0 DaCommen t '20ppt > LCS " Annotation : ~ Sampl eIndex : Sampl eType ; Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois ePercentage ; Base . Suta . Windo * Peak-Split . Fact o Repor t Larges t P e Min . Pea k Height : Min . Pea k Width : Smoothin g Width : R TWindow ; Expecte dRT: Us eRelativ eRT : Int . Type : Retentio nTime : Area : 2 0 ; Height : 2 Star t Time : En dTime : 3 4 OC

19. 9 20. 8 7/31/201 1 4:51:1 7 A M Ye s Specify P

a 5 0 : 1.0 0 c : 2 ak ; Ye s 0.0 0 0.0 0 0 30. 0 16. 2 N o Manua l 16. 1 n 7956 6 co t .3Set00 6 c 16. 4 R



# I Sampl e Nam* : "k110729b04* - Sampl e ID : •lCS-110726-i rFil* : •k110729b.»tf r P*a k Name : "PFOS " Ma3s(ee) : '499.000/99.00 0 Da.499.000/80.00 0 Da,499.000/130.00 0 Da Commen t '2pp b LC S ECF " Annotation : ~ SaDq:>l e Index : 4 4 Sampl eType : Q C Concentration : Calculate dCone : Acq . Date : Acq . Time : Modified : Proc , Algorithm : Nois ePercentag e 0.19 8

0.27 2 7/31/201 1 7:19:0 5 Ye s Specify 5 0 ng/m

L ng/m L A M Paramet e Base . Sub . Window ; 1.0 0 Peak-Split . Factor : 2 Repor t Larges t Peak : Ye s Hln . Pea k Heigh t Min . Pea k Width : Smoothin g Width : R TWindow ; Expecte dRT : Us eRelativ eRT : Int , Type : Retentio nTime : Height : 2 Star t Time ; En dTime : 0 0 30. 0 16. 2 N o Manual 16. 1 464 4 .lle+0 0 15. 9 16. 3 mi n 0 0 cp s 0 0 se c p o ml n
ml n c p s ml n ml n 2.1* 4 2.0* 4 1.9* 4 1.8* 4 1.7e4 1.6* 4 1.5* 4 1.4* 4 1,3* 4 1,2* 4 1.1* 4 1,0* 4 9000. 0 8000. 0 7000. 0 6000. 0 5000. 0 4000. 0 3000. 0 2000. 0 1000. 0 0. 0 •MhM i 16,1 5

15.99s . 

# I I 1 1 H 

# • ^B^ie ^ 

# ',''"lf l ilf k JA^HAI , Sampl eType : Concentrati o Calculate dC Acq . Date : Acq . Time : Modified : Proc . Algor i Nois ePerce n Base . Sub . W Peak-Split . hm :

a g e O

C 1.9 8 2.2 6 7/31/201 1 8:22:2 5 A

l Ye s Specify P 5 0 ndow : 1.0 0 3r : 2 Repor t Larges t Peak ; Ye s Mln . Pea k H e g h t Min . Pea k Width : Smoothin g M

l R TWindow : Expecte dRT : UseRelativ e Int . Type : Retentio nTl Area : Height : Star t Time : En dTime : ith :

RT :
1 4 ng/m

L ng/m L

aramet e mi n 0.0 0 cp s 0.0 0 se c 0 30. 0 16. 2 N o Kanua l 16. 1 557 0 C O .E3e+00 5 15. 8 16. 3 p o

ml n
ml n n t s c p s ml n ml n

14. 0 14. 5 15 5 la o 16. 5 17. 0 17. 5 18. 0 18 5 

### Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , Pag e5 o f 7 Pag e 8 8 of11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S I n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0729b.rd b

l||Samp<*Nam*:"k110729lfl4 r Sampt e ID:-t.CS-110726-16 r Fli*:1c110729b.wif r • Pea k Nam* : "PFOS ' Irias3(«s) : '499.000/99.00 0 Da,499.000/80.00 0 Oa,499.00a'130.00 0 Da ' • Comment : '20pp b LC S Ec r Annotatkm : - Sampl e Index ; 4 7 Sampl e Type : Q C Concentration : 19. 8 ng/m L Calculate dCone : 22. 9 ng/m L16* 6 Acq . Date : 7/31/201 1 Acq . Time : 9:25:4 5 A M 15* 6 Modified : Ye s Ptoc . Algorithm : Specif y Parameter s - M O II I, . ^ Nois e Percentage : 5 0 '•** ^ Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 2 )_3 ^ Repor t Larges t

Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se c 1-2* 6 Smoothin g Width : 0 point s R TWindow : 30. 0 se c . . ^ Expecte dRT : 16. 2 mi n'•'" ° Us e Relativ e RT ; N o 1.0* 6 Int . Type : Manua l Retentio nTime : 16. 1 rain , Area : 1652749 3 count s g " S-'* ' Height : 1.66e*00 6 cp s > : Star t Time : 15. aml n| g( ^ En dTime : 16. 4 mi n| 7.0* 5 6.0* 5 5.0* 5 4.0* 5 3.0* 5 2.0* 5 1.0* 5 16.1 5

16.01 ^ 

# I I 

# 1 • M . 14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 17 5 18. 0 18 5 1 SampleName:-k110729b08 r Sampk t

ID:"GLP1CKI1-02-1U»8
- File : "k l 10729b.wt r ~~ " T\m^.nir \ 1 P*a k Name : 'PFOS ' hta3S{*s) : "499.00099.00 0 Da.499.000/80.00 0 Da,499.00(V130.00 0 Da * • Comment:"DALGW137L0110624 ' Afmotatbn: " Sampl e Index : 8 1 Sampl e Type : Unknow n Concentration ; N/ A24B S Calculate dCone : 1.9 3 ng/m L Acq . Date : 7/31/201 1 2 3* 5 Acq . Time : 9:24:5 9 P M 2.2e 5 Modified : Ye s Proc . Algorithm : Specif y Parameter s - M Q II I2.1B 5 Nois e Percentage : 5 0 Base . Sub . Window : 1.0 0 mi n^"" ^ Peak-Split . Factor : 2 ^ g ^ Repor t Larges t

Peak : Ye s Mln . Pea k

Height ; 0.0 0 cp s 18* 5 Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 1.7* 5 R TWindow : 30. 0 se c Expecte dRT : 16. 2 mi nl-*« 5 Us e Relativ e RT : No ^ 1 Int . Type : Manua l 4 j ^ Retentio nTime : 16- 1 ml n« Area : 217245 2 count s & 1.3* 5 Height : 2.43etO0 5 cp s ^ Star t Time : 15. 8 mi n" W1.2* 5 En dTime : 16. 4 mi ng 1 1.1* 5 1.0* 5 9.0* 4 6.0* 4 7.0* 4 6.0* 4 5.0* 4 4.0* 4 3.0* 4 2.0*4
- 1.0*4
- 161 4

16.00 ^ 

# I I 

# • • 

# • M . 14. 0 14. 5 15, 0 15 5 16. 0 18. 5 17. 0 17 5 18. 0 18 5 1 Tlme.mt n IP*a k Nam* : -PFOS " Iltas3<*8) : '499.000/99.00 0 De,499.000/80.00 0 Da,499.000/130.00 0 Da ' Sampl e Index : 7 2 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : 0.49 8 ng/m L^^ * Acq . Date : 7/31/201 1 Acq , Time : 6:14:2 7 P M *'^ * Modified : Ye s 3.8« 4 Proc . Algorithm : Specif y Parameter s - M Q II I Nois e Percentage : 5 0 3.6* 4 Base . Sub . window : 1.0 0 ml n Peak-Split . Factor : 2 3.4« * Repor t Larges t

Peak : Ye s Min . Pea k

Height : 0.0 0 cp s 3.2* 4 Mln . Pea k

Width : 0.0 0 se c Smoothin g Width ; 0 point s 3.0* 4 R TWindow : 30. 0 se c Expecte dRT ; 16. 2 mi n2.8* 4 Us e Relativ e RT : No Z6* 4 Int . Type : Manua l Retentio nTime : 16- 1 ml nj , Z4* 4 Area : 41408 3 count s g " Height ; 1.29e-t00 4 cp s > : 2,2* 4 Star t Time : 15- 8 ml nft En dTime : 16. 3 rain J2.0* 4 1.8* 4 1.6* 4 1.4* 4 1.2e 4 1.0* 4 8000, 0 6000. 0 4000, 0 2000. 0 161 4

15,99. .

# 1 1 15.65.. M 

# ^^j m 14. 0 14 5 15. 0 15 5 16. 0 IPea k Name ; "PFOS
- Mas9(*s) : •499.000«9.00 0 Da,499.000/80.00 0 Da.499.000/130.00 0 Da ' • Comm*nt'DALGW138L011063ff ' Annotatton: " Sampl e Index : 8 6 Sampl e Type : Unknow n Concentration : N/ A, . , Calculate dCone ; 0.84 0 na/m L"•'" ^ Acq . Date : 7/31/201 1 50, 4 Acq . Time : 11:10:4 7 P M 4.8e 4 Modified : Ye s proc . Algorithm ; Specif y Parameter s - M Q II I^•°" * Nois e Percentage : 5 0 4.404 . Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 2 4.2*4
- Repor t Larges t

Peak : Ye s .^ ^ Min . Pea k

Height : 0.0 0 cp s Min . Pea k

Width : 0.0 0 se c 3.8* 4 Smoothin g Width : 0 point s R TWindow : 30. 0 se c 3-6» 4 Expecte dRT : 16. 2 mi n- > ^ J Us e Relativ e RT : No ^'^ ^ 3.2e 4 Int . Type : Manua l Retentio nTime : 16. 2 ml n„ ^•"' * Area : 56638 3 count s & 2.8e 4 Height : 5.25e+00 4 cp s ^ Star t Time : 15. 8 ml nf 2.6e 4 En dTime : 16. 3 ml nS 1 24e 4 2 A 4 2.0e 4 1.8e 4 1.6e 4 1.4* 4 1.2e 4 1.06 4 8000. 0 6000. 0 4000.0
- 2000.0
- 16.5 2 17J 4



# w/V,..,., ^ ^mJK.^.f. ^ 1 6 5 17. 0 17 5 18. 0

16.1 A

16.0 U


# 1 

# 1 I 

# • 

# I • l l

.. M 14. 0 14 5 15. 0 15 5 16. 0 I17.77



# l\ . 

# J-J v .. . 1 6 5 17. 0 17 5 18. 0 ' y ^ 1 18 5 j

1 8 5 Dat aprinte db y ST

W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e6 o f 7 

### Pag e 8 9 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729b.rd b

•Sampl e Name:'k11072Sb095 ' Sampt o ID:'GLP1(K}1-02-18-145 ' I Pea k Nam* : 'PFOS ' Iillas3(ett) : '499.000/99.00 0 Oa,499.000/80,00 0 Da. 4 •Comment:'DALGW143R0110622-ISadd*< rAnnotatkin : " ^ Sampl e Index : 9 5 Sampl e Type : Con < atlo E N/ A Calculate dConc : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e 23. 4 8/1/201 1 2:21:2 5 A

M Ye s Specify P

a S O Base . Sub . Window : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t

p Mln . Pea

k Heigh t Mln . Pea

k Width : Smoothin g Width ; R T Window : Expecte dRT : Us e Relativ e RT : Int . Type : Retentio nTime : Area : 2 1 ak : Yes 0.0 0 0.0 0 0 30. 0 3 ng/m

L

mi n cp s

e c 16. 2 mi

n Ko Manua l 16. 0 n 6380 6 co t i n

n t s 1.7* 6 1.6* 6 1,5* 6 1.4* 6 13* 6 1.2* 6 1.1*6
- 1.0* 6 9.0* 5 8.0* 5 7.0* 5 6.0e5
- 5.0e5
- 4.0e5
- 3.0e5 2.0e5
- 1.0e5
- 0.0
- 14. 0 14 5 1S. 0 15 5 16. 0 16. 5 17. 0 17 5 18. 0 16 5 1 Sampl*Nam*:n<110729t>10( rSampl a ID:-GLP1&O1-02-18.150 - FB . -wia729b.wi r I Paa k

Nam*:'PFOS - Masa{*a):-499.000/99J}00Oa,499.000/80.00ODa,499.0D0/13D.000Da " •Commant'DALGW144S0110628-lSadd*d ' Sampl e Index : 10 0 Sampl e Type : Unknow n Concentration : N/ A Calculate d Conc : 29. 5 ng/m L Acq . Datft : 8/1/201 1 Acq . Tlmo : 4:07:2 1 A M Modified : Ye s Proc . Algorithm : Specif yParameter s - Kols e Percentage : 5 0 Base . Sub
. Window : 1.0 0 mi n Peak-SplU
. Factor : 2 Repor t Larges t Peak : Ye a Mln . Pea k

Height : 0.0 0 cp s Mln . Pea KWidth : 0.0 0 se c Smoothin g Width : 0 point s R TWindow : 30. 0 se c Expecte d RT : 16. 2 mi n Us e Relativ e RT : N o Int . Type ; Manua l Retentio n Time : 16. 0 ml n Area : 1500423 1 count s Height : 1.24et00 6 cp s Star t Time : 15. 7 mi n En d Time ! 16. 4 mi n Annotatton: "

M Q II I 1.20*

6 1.15* 6 i.ioe e 1.05e B 1.00e 6 9.S0e 5 9.00* 5 850* 5 6.00e S 750* 5 .00. 5 ^ . 650* 5 1 6.00*

5 1 550* 5 SJOOe S 450* 5 4,00* 5 350* 5 3.00* 5 250e 5 2.00e 5 1.50e 5 1.00B 5 5.00e 4 0.0 0 ^5A ^

14. 0 14. 5 15. 0 15. 5 16. 0 16 5 17. 0 17 5 18. 0 18 5 Tlnw . m h

Dat aprinte db y ST W Printin g Time : 12:10:5 8 P M Printin g Date : Tuesday , Septembe r 06 , Pag e7 o f 7 

### Pag e 9 0 o f11 3GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Workstation : ETSKIRK Result s Name : kll0729 bext.rdb

Dat a worke d up byKJ U Pag e 1 o f 1 Printin g Time : 12:04:3 6 PM Printin g Date : Wednesday , Augus t 03 , 201 1 

## Pag e 9 1 of 11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS
. PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 Result s Name : kll0729 b ext.rd b

Sanv>leName:l(11072gb019 ' Sampl e 10 : Pea k Name : 'PFOS ' Mss9(*e) ; '499.00Q'99 J Comnwn t

1 0 t^M LF C O dhSyrth , Wat* ^
Calc u Acq .

15. 0 15 5 16. 0 16 5 SamF'eNBine:*kll0729l>02 r Sampt*ID:"1 1 002-243 ' File:*k110729b.wn r Pea k Name : -pFOS * Mass(*s) : '499^)0/99,00 0 D a.499.000/60.00 0 Ds.499.00a/130.00 0 Da * Comm*n t

"Metho dBtank " Annotatkin : ~ Sampl e Index : 2 7 Sampl e Type : Concentration ! N/ A Calculate dConc : 0.65 3 ng/m L Acq . Date : 7/31/201 1 Acq . Time : 2:23:3 0 A M Modified : Ye s Proc . Algorithm : Specli y Parameter s Noir e Percentage : 5 0 Base . Sub . Window : 1.0 0 ml n Peak-Split . Factor : 2 Repor t Larges t

Peak : Ve s Min . Pea k

Height : 0.0 0 cp s Min . Pea k

Sldth : 0.0 0 ae c Smoothin g Width : 0 poin t R TWindow : 30. 0 ae c Expecte dRT : 16. 2 ml n Us e Relativ e RT : No Int . Type : Manua l Area : 2392 3 count s Height : 4.26e+00 3 cp s Star t Time : 16. 1 ml n - M Q II I



## 1 1 4400- ) 4200
- 4000
-
3600
-

2600
- 2400
- 2200
-
1800
- 1600
- 1400
- 1200
- 1OO0
- 

# I Sampl e Name : 1(110729l>034
- Sampl e ID : XCS-110726- r Fi*:-k110729b.w4 r Pea k Nam* : -PFOS " Maaa(*8) : '499.000/99,00 0 D a,499.000/80.00 0 D a.499.000/130.00 0 Oa * Commen t

"TOppb LCS ' Annotation : ~ Sanpl e Index : 3 4 Sampl e Type : O C Concen t

ra t Ion : 19. 9 ng/m L Calculate dConc : 21. 4 ng/m L Acq . Date : 7/31/201 1 Acq , Time : 4:51:1 7 A M 18. 0 18 5 

# I Sampl e Name : 1(1107291)031
- Sam p Paa k Nam* : 'PFOS * Mas9{*«) ; '499 . Comm*nt : '2pp b LCS ' Annotatton: " Sampl e Index : 3 1 Sampl e Type : Calculate dConc : Acq . Date : Acq . Time : 1,9 9 ng/m L 2,0 7 ng/m L 7/31/201 1 3:47:5 5 A

M N o ; Pe n Peak-Split . Factor : Repor t Larges t

Peak : Kin . Pea k

Height : Mln . Pea k

Width : Smoothin g Width : R TWindow : 3 1 Expecte dRT : Us e Relativ e RT : r
Height : Star t Time ; [4 947 4 8 C ( 2.53e+00 5 16. 1 2.5e S Z4* 5 2.3* S 2.2* 5 2.1* 5 2.0*5
- 15*5
- 1.8*5
- 1.7*5
- 1.6* 5 1.5* 5 1.4e 5 1,3*5
- 1.2*5
- l.leS
1.0*5
- 9.0*4
- 8.0* 4 7.0* 4 6.0*4
- 5.0e4
- 4.0e4
- 3,0e4
- 2.0*4
- 1.0*4
- Modified : Y Proc . Algorithm ; Spe c Nois e Percentage : Base . Sub
. Window ; Peak-Split
. Factor : Repor t Larges t Peak : Mln . Pea k

Height : Mln . Pea k

Width : Smoothin g Width : R TWindow : 3 0 e a f yPa 5 0 1.0 0 2Ve s 0.0 0 0.0 0 0 0

Area : 2037777 0 Height : 3.35e+0 C Star t Time : 16. 1

15. 0 15 5 16. 0 16 5 Tkne.mt n 18. 0 1B 5 Dat aprinte db y ST

W Printin g Time : 12:13:0 0 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 o f 2 

## Pag e9 2 o f 11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0729 b ext.rd b 

# I Sampl a Nam* : "k110729t)044 ' Sampl e ID : •LCS-110726-13 " File : •k110729b.wi r Pea k Name : "PFOS * Ms9s{*8) ; *499.00Q/99.00 0 D*,499.000/80.00 0 Da.499.00CV130.00 0 Da ' Conunen t"^p b LC S ECF ~ Annotation: " 1.9 8 I 2.0 4 r 7/31/201 1 8:22:2 5 A

M Modified : Proc . Algorithm : Nois e Percentage : Base . Sub
. Windo w Peak-Split
. Fact o Repor tLarges tPeak : ' Mln . Pea k

Height : i Mln . Pea k

Width : ( Smoothin g Width : I R TWindow : 30. 0 Expecte d RT : 16. : Us e Relativ e RT : N o ;lf y Pa ] 5 0 1.0 0

, Typ e Mani . 16. 1 Area : 146407 4 c c Height : 1.53e»00 5 Star tTime : 15. 8 En d Time : 16. 3 1.Se5 1.4* 5 15* 5 1.2* 5 1.1* 5 iJOt S 9.0e 4 8.0e 4 7.0e 4 6.0e 4 S,0* 4 

# I San4)leName:K110729b055 - Sampi s ID:-GLP1(M>1-02-18-02 1 Pea k Nam* : 'PFOS ' Ma>a(**) : -499.000i99.00 0 Da,499.000/80.00 0 Da , Comment : "DA LG W131 R 011062 2 (dl l 1:100) " Annotation : - Sampl e Index : 5 5 Sampl e Type : Unknow n Concentration : N/A Calculate d Conc : 1530 . ng/m L.--^ . 1.20* 6 1.15* 6

' - " ^ ' " 1.10* 6

1.05* 6 1.00* 6 9.50*5 - nt s 9.00e 5

8.S0e S 8.00* 5 7.50* 5 § - 7.00e 5 ^ 6.S0e 5 I &00* S 5.50* 5 5.00*5
- 4.50* 5 4.00* 5 3.50* 5 3.00* 5 Z50* S Z00e 5 1.50*5 l.OOe S 5.00* 4 O.O 0 M10729b.*lf r ,499.000/130.00 0 Da 

# I Sampl * Nam*:T<110729W>4 rSampl * ID : "LCS-110726-ie " Fil* : "k110729b.wi r P*a k Nam* : 'PROS ' Mas9(*s) : '499,000/99.00 0 Da.499.000/80.00 0 Da.499.000/130.00 0 Da ' Conunen t '20pp b LC S EC rArawtafion: " Sampl e Index : 4 7

1.5* 6 1.4* 6 1.3* 6 l,2e 6 l.leS
1, 0 Sampl e Type : Concentration : Calculate d Conc : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e Q C 19. 8 17. 2 7/31/201 1 9:25:4 5 A M Ye s Specif yPa r 5 0 Base . Sub . Window : 1.0 0 Peak-Split . Fac t r : 2 Repor t Larges t Peak : Ye s Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : 0.0 0 0.0 0 0 30. 0 a 16. 2 m N o ig/mL

ng/m L

mi n cp s se c p o

, n
Height : Star tT i En d Tim < .G6e*00 e cp s 9.0*5
- 8.0* 5 7.0* 5 6.0e 5 5.0e 5 4.0*5
- 3.0* 5 2.0* 5 1.0* 5 0, 0

Acq . Date : Acq . Time : Modified : Proc . Algorithm : Nois e Percentag e 7/31/201 1 12:14:44 P Ye s Specif yP a 5 0 Base . Sub . Window : 1.0 0 Peak-Split , Fac t or : 2 Repor t Larges t Peak : Ye s Min . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : E>:pecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Area : 1 4 Height : 0.0 0 0.0 0 0 30. 0 16. 2 n N o Manua l 16. 0 n 18726 5 C O ,30e+00 6

Dat aprinte db y ST

W Printin g Time : 12:13:0 0 P M Printin g Date : Tuesday , Septembe r 06 , Pag e 2 o f2 201 1 

### Pag e 9 3 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0802a.rdb

m 

# 1 w C O 

# < 

# i C O 

### c 

# < 2 7 J 26 - 25 - 24 - 23 - 22 - 2 1 - 20 - 19 - 18 - 17 - 16 - 15 - 14 - 13 - 12 - 11 - 10 - 9 8 - 7 - 6 - 5 - 4 - 3 - 2 1 - 0 - 1 2 1 4 Analyt e Gone
. /

I S Conc
. Dat a printe d b

ySTW Printin g Time : 12:14:0 9 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e 1 o f 1 

## Pag e 9 4 o f 11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kirk Result s Name : kll0802a.rd b

li|sampl*Nam*:'k110802a013 - Sampl e ID:'1 1 003.2M - F^:-k110802a.«4 r I Pea k Nam* : '13C8PF0S-IS(IS) - Ma«s(*s) : -S07.000ffl0.00 0 Da ' •Commant : "0. 1 ng/m LF C M i n Synth
. Wat*! * Annotatkin : ~ Sampl e Index : 1 4 Sampl e Type : Standar d , 8 2 0 Concentration : 0.96 0 ng/m L100* 5 Calculate d Conc : N/A Acq . Date : 8/2/201 1 950« 4 Acq . Time : 7:35:0 9 P M Modified : N O ^"" ^ Proc . Algorithm : intelllQua n - IQ AI I Mln . Pea k

Height : 0.0 0 cp s 8.50* 4 Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 8.00* 4 R TWindow : 30. 0 se c Expecte d RT : 16. 2 ml n 7.50e 4 Us e Relativ e RT : N o 7.00e 4 Int . Type : Valle y Retentio n Time : 16. 2 ml n 650* 4 Area : 62667 1 count s Height : l.OletOO S cp » g^ . Star t Time : 16. 1 ml n "•"*" * En d Time : 16. 4 mi n ^^ ^ f5.00e 4 1 4.50e 4 4.00e 4 3.50e 4 3.00e 4 2.50e 4 2.00e4 - 1.S0e4 - 1.00e4 - 5000.0 0 14. 0 14. 5 1^. 0 15. 5 16. 0 L , i-''*'^^ ' 16. 5 17. 0 17. 5 18. 0 16, 5

•Sampl e Mam*:T(110802a025 " Sampl * ID:-LCS-110726-1 " Fle:-k110802a.«i r I Pea k Name : "13C8PFOS-IS{IS) - Maas^ea):-507.00are0.00 0 Da ' I Commen t •0.2pp b LCS " Annotatbn: " Sampl e Index : 2 6 Sampl e Type : Q C ia2 0 Concentration : 0,95 3 ng/m L115* 5 Calculate d Conc : N/A Acq . Date : B/2/201 1 110* 5 Acq . Time : 11:48:1 5 P M 1.05*5 Modified : N o Proc . Algorithm : IntelllQua n - IQ AI I 1,00*5 Min . Pea k

Height : 0.0 0 cp s Mln . Pea k

Width : 0.0 0 se c950* 4 Smoothin g Width : 0 point s R TWindow : 30. 0 se c9.00e 4 Expecte d RT : 16. 2 ml n „ us e Relativ e RT : N o '^•^°» * Int . Type : Valle y8.00*4 ' Retentio n Time : 16. 2 mi n TtOi, ^ Area : 69397 5 count s Height : 1.16e+00 5 cp a 700*4 - Star t Time : 16. 1 ml n En d Time : 16. 4 ml n „ 650e4 . g ; 6.00*4 . 1 5.50*4 - 5.00*4 - 4.50*4 - 4.00e4 - 3.50e 4 3.00* 4 ^50* 4 2.00* 4 1.50* 4 1.00*4 - 5000.00 - 14. 0 14. 5 15. 0 15. 5 16X ) 1 Tim * 16 5 17, 0 17. S 18. 0 18. 5 m l n HsampUName:T.110S02a02 rSampt e ID:'1 1 OOS-129 - Fle:-k110802a.v>t r I Pea KNam*:-13C8PFOaJS(IS) - Ma»(*s):'507.000/80.00 0 Da ' •Commen t "Metho d Btank " Annotatkin : " Sampl e Index : 2 4 Sampl e Type : Unknow n ,62 3 Concentration : 0.96 0 ng/m L Calculate d Conc : N/ A Ac;q . Date : 6/2/201 1 1.4e 5 Acq . Time : 11:06:0 9 P M Modified : Ye a 1.3e 5 Proc . Algorithm : IntelllQua n - IQ AI I Mln . Pea k

Height : 0.0 0 cp s Mln . Pea KWidth : 0.0 0 se c1.2e 5 Smoothin g Width : 0 point s R TWindow : 30. 0 se c Expecte d RT : 16. 2 ml n Llo S Us e Relativ e RT : N o Int . Type : Valle yI.Oe S Retentio n Time : 16. 2 ml n Area : 95717 0 count s Height : 1.47e-l-00 5 cp s H-°« ^ Star t Time : 16. 1 ml n En d Time : 16. 5 ml n « 1 8.0e 4 1 ^-0 ^ £ 6.0* 4 5.0* 4 4.0* 4 3.0* 4 2.0e4 ^ 1.0e4 - 14. 0 14. 5 15. 0 15. 5 16. 0 16. 5 17. 0 17. 5 18. 0 18. 5 1

•Sampl*Name:1(110802a028 ' Sampl e ID:TCS-11072fr4 - Fle:1<110802a.wlf r •Pea k Name : '13C8PFOS-IS{1S) ' Mas9(es): "507.000/80,00 0 Da ' •Commen t '2pp b LCS * Annotatton: " Sampl e Index : 2 9 Sampl e Type : Q C ,6, 0 Concentration : 0.95 3 ng/m L Calculate d Conc : N/ A1.15* 5 Acq . Date : 8/3/201 1 Acq . Time : 12:51:4 5 A M l.lOe S Modified : N o ^^^ ^ Ptoc . Algorithm : IntelllQaa n - IQ AI I 1 nn. s Mln . Pea k

Height : 0.0 0 cp s ''" " Mln . Pea k

Width : 0.0 0 se c9 50* 4 Smoothin g Width : 0 point s R TWindow : 30. 0 se c900*4 - Expecte d RT : 16. 2 ml n Us e Relativ e RT : N o 8.S0e4 - Int . Type : Valle y8.O0e4 - Retentio n Time, : 16. 2 ml n Area : 74279 2 count s 7.S0e4 - Height : l.iee+00 5 cp s Star t Time : 16. 1 ml n (.u« t En d Time : 16. 4 ml n g

_ ^^^ .
1 5.50* 4 S.00e4 - 4.S0e4 - 4.00e4 - 3.S0e 4 3.00e 4 2.50e 4 Z0ae 4 1.50e 4 1.00e4 - 5000.00 - 14. 0 14. 5 15. 0 15. 5 16, 0 

# L 16. S 17. 0 17. 5 18. C Time , m m 18. 5 

### Dat aprinte db y ST

W Printin g Time : 12:15:3 8 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e1 o f 5 Pag e 9 5 o f11 3

### GLP10-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS
. PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0802a.rd b 

# 1 Sanv)t*Name:-k1108O28O3 rSampl*lD:'LCS-110726- rFl*:1(110802a.w(i r P*a k Nam* : '13C8PFOS-IS(IS) ' Mas9(c«) : '507,000/80.00 0 Da * Commerd : '20pp b LCS " Annotation : ~ Sampl e Index : 3 2 Sampl e Type : •Q C Concentrati o Calculate dC Acq . Date : Acq , Time : Modified : Proc . Algor i Mln . Pea k H e h m q h Mln . Peak

Widt h Smoothin g W

l R TWindow : Expecte dRT : Us eRelativ e Int . Type : Retentio nT l Area : Height : Star t Time : En dTime : R

T 0.95 3 N/ A e/3/201 ] 1:54:5 8 No n g / A M IntelllQua n- ! 0.0 0 0.0 0 0 30. 0 16. 2 H o Valle y 16. 2 9S842 3 c 1.59e-f00 5 16. 1 16. 4 poin t

ml n
ml n ountB c p s ml n ml n



# I SampleName:'k110802a041 " Sampl e ID:*LCS-110726-13 - Fie : "ki t 0602a . wi r Pea k Nam* ; -13C8PFOS-IS(IS} ' MBSS<*S) : "507.000/60.00 0 Da ' Common! : "2pp b LC S ECF " Annotatton:" " Sampl e Index : 4 2 Sampl e Type : Q C 0.95 4 ng/m

L 

# I SampleName:'k11O802a044 " Sampl * ID:'LCS-110726-16 ^ ^ile:'kl10802a.wi r P*a k Nam* : '13C8PFOS-IS(IS} ' Mess(«s} : ''507.000re0.00 0 Da ' Comment'20pp b LC S EC rAnnoUtton: " Sampl eIndex : 4 5 Calculate dConc : N/ A Acq . Date : 8/3/201 1 Acq . Time : 5:26:1 1 A M Modified : Ye s Proc . Algorithm : IntelllQua n- IQ AI I Mln . Pea k Height ; 0.0 0 Min . Peak

Width : 0.0 0 Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 16. 2 Us eRelativ eRT: N o Int . Type : Valle y Retentio nTime : 16. 2 Area : 63982 8 c Height : 1.08et00 5 Star t Time : 16. 1 En dTime : 16- 4 cp s point s mi n
ml n c p s ml n ml n 

## i 1.0S*S

9.50e 4 0.00e'
- 8.50*'
- 8.00*'
- 7.50*4
- 7.00*-
- 6.S0e'
- 6.00e4
- 5.50e4
- 5.00e4
- 4.S0e4 : 4.00e4 : 3.S0e4 ^ 3.00* 4 2,50* 4 2,00* 4 1.50e 4 1.00e4- i 5000.00- i 0.00 ^ Sampl eType ; Concentration : Calculate dConc : Ac;q. Date : Acq . Tlma: Modified : Proc . Algorithm : Mln . Pea k Heigh t Mln . Peak

Width : Smoothin g Width : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : O C 0.95 3 N/ A B/3/201 1 6:29:3 0 A

M Ye s IntelllQu a 0.0 0 0.0 0 30. 0 s 16. 2 n No Bas eT o Ba s 16. 2 n Area : 69932 7 cour Height : Star t Time : ,14e+00 5 t

16, 1 n

15. 5 16. 0 16. 5 I.IOe S 1.05* 5 1.00* 5 S.50*4 S.00*4
- 6.50e4 < 8.00e4 < 7.50e4
- 7.00*4
- 6.50*4
- ' { 6.00* 4 I5.50*4 '

= 5.00*4
- 4.50*4
- 4.00*4
- 3,50*4
- 3.00*4
- 2.50*4
- 2.00e4
- 1.50*4
- 1.00*4
- 5000.00
- 0.00
- Dat aprinte db y ST

W Printin g Time : 12:15:3 8 P M Printin g Date : Tuesday , Septembe r 06 , Pag e2 o f 5 

### Pag e 9 6 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k Result s Name : kll0802a.rd b

Modified : Proc . Algorithm : Mln . Pea k Heigh t Mln . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Ye » IntelllQua n 0.0 0 c 0.0 0 3 0 p o 30. 0 s 16. 2 m Saii^Nam*:'k110602a07 rSampl * ID : "GLP10O1-0M6-133 ' File:"kl10802a.wl T P*s k Nam* : "13C8PFOSJS(IS ^ Ma33{*s) : •507.000re0.00 0 Da ' Comman t "DA LGW130 LRB110630 " Annotatton: - Sampl e Index : 7 3 Sampl e Type : Unknow n Concentration : 0.96 0 ng/m L Calculate d Conc : N/ A Acq - Date : 8/3/201 1 2,0*5 - Acq . Time : 4:21:0 5 PM 1.9e5 - I I1.8*5
-

1.7*5 - 1.6*5 - 1.5*5 - 1.4*5
- 1.3eS 1.2e5 - k l.le S 

## I 9.0e4 - 8.0e4 - 7.0e4 - 6.0e4 - 5.0e4
- 4.0e4
- 3.0e4
- 2.0e4
- 1.0e4 - Helght : Star t Tlm i En dTime :

0. 0 14. 0 14. 5 Sampt * Name:-1(11O8O2B013 - Sampl e ID:'1 1 00^25-5 " Rle;"kl10802a.wti r Paa k Nam* : "PFOS " Mass(*s) : "499.000/99.00 0 Da,499,000/80.00 0 Da,499.00CV130.00 0 Da ' ComnMnt : T). 1 ng/m LF C st d i n Syntt)
. Water " Annotatton:" " Sampl e Index : 1 4 Standar d 0.10 0 nq/m L 0.096 5 ng/m L 1.25*4
- 1.20* 4 1.1S*4 - s - M Q II I1.10* 4

1.05* 4 1.00* 4 95oao o nt s 9000.0 0

8soao o 8000.0 0 75oao o S . 7000.0 0 ^6500.0 0 I6000.0 0

~ 5500.0 0 5000.0 0 4500,0 0 4000,0 0 3500.0 0 3000.0 0 2500,00 ' 2000.0 0 isoao o 1000.00 ' 5oao o 0.00 - 

# I Sampl * Name : "k1108CCa073 ' Sampl a ID : "GLP1IW)1-02-18-134 " File : "k110802a.wi r Pea k Name : "13C8PFOS4S(IS} ' Mas3<ea) ; -507.000/80.00 0 Da " Comm*nt'OALGW133SRB11063ff ' Annotatton: ' Sampl e Index : 7 4 Sampl e Type : Unknow n 0.96 0 ng/m L N/ A Acq . Date : Acq . Time : Modified : Proc . Algor : Mln . Pea k H e thm :

ight ; Min . Pea k Width : Smoothin g W R T Window : Expecte d R T Us s Relativ e Int . TiT.e : Retentio n T : Area : Height : RT :

12 8 2 8/3/201 1 :42:1 6 P M Ye s ntBlll Q 0.0 0 0.0 0 0 30. 0 16. 2 N o Valle y 16. 1 34 1 C 12«-t00 5 a n

m l

m l c p 2,1* 5 Z0e 5 1.9e 5 1.8e5
- 1.7* 5 1.6* 5 1.5e5
- 1.4* 5 1.3* 5 1.2* 5 1.1* 5 1.0*5 < 9.0*4
- 8.0*4
- 7.0*4
- 6.0*4
- 5.0*4
- 4.0e4
- 3.0e4
- 2.0e4 < 1.0e4
- 0.0
- 14. 0 14. 5 SanvileName:*k110802s02 rSamp4elD:'1 1 005-129 " Flle:-kl10802a.wt r Pea k Name : 'PFOS - Mass<*s) ; '499.000/99,00 0 Oa,499.000/80.00 0 Da.499.000/130.00 0 Da Commen t -Mettw d Btanl TAnnotatton:" " Sampl e Index : 2 4 Sampl e Type : Unknow n Concentration : N/ A 16. 0 16. 5 17. 0 Tlme.mi n 17. 5 18. 0 18. 5

Sampl e Type : Acq . Date : Acq . Time : Modified : Proc . Algo r Hols e Perc e thm : Base . Sub . Wind o Peak-Split . Repor t Lar g Fac t

S t P Min . Pea k Heigh t Min . Pea k Width : Smoothin g Width : R T Window : Expecte d R T Us e Relativ e RT : Int . Type : Retentio n T Height : Star t Time : End Time : a/2/201 1 7:35:0 9 P M N o Specif yP a 5 0 J : 1.0 0 ak : Ye s 0.0 0 0.0 0 0 30. 0 a 16. 3 n N o Valle y 16. 2 n 772 4 2 cou r .30e-f00 4 c 16. 1 n 16. 3 a

••^i^..^ » thili*«l<ii*^H^*««|iM< > Calculate d Conc : Acq . Date : Acq . Time : Modified : Proc . Algorithm : Hols e Percentag e < 0 8/2/201 1 11:06:0 9 P M Ye s Specif yPa r 5 0 Base . Sub . Window : 1.0 0 Peak-Split . Fact e Repor t Larges t Pe Mln . Pea k Heigh t Min . Pea k Width : Smoothin g Width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : t : 2 ak : Ye s 0.0 0 0.0 0 0 30. 0 3 16. 3 m N o Manua l 16. 2 m Area : 1645 0 cou n Height : 2 Star t Time : En d Time : .16e•^00 3 c p 16. 0 m 16. 3 m

16. 0 16. 5 Tim* , ml n 16. 0 16. 5 Time , mk

) Dat aprinte db y ST

W Printin g Time : 12:15:3 9 P M Printin g Date : Tuesday , Septembe r 06 , Pag e3 o f 5 

### Pag e 9 7 o f11 3GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1 •ETS-Kir k Result s Name : kll0802a.rd b

|||SamplaNam.:-K110e02a025 ' Samp)
. ID:"LCS-11072ft- rFie:-k110802a.»li r I Pea k Nam* : -PFOS " Ma9s(*«) : '499.OOCW9.00 0 Da,499.000ffl0.00 0 Da,499.000/130.00 0 Da ' •Commant : "0.2pp b LCS " Annotatton : " Sampl e Index : 2 6 1 Sampl e Type : Q C Concentration : 0.19 9 ng/m L Calculate d Conc : 0.21 9 ng/m L Acq . Date : 8/2/201 1 Z8* 4 Acq . Time : 11:48:1 8 P M Modified : N o 2.6* 4 Proc , Algorithm : Specif yParameter s - M O II I Nois e Percentage : 5 0 Base . Sub
. Window : i.O O mi n 2.4e 4 Peak-Split
. Factor : 2 Repor tLarges tPeak : Ye s Min . Pea k

Height : 0.0 0 cp s 2.2* 4 Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s R TWindow : 30. 0 se c2.0* 4 Expecte d RT : 16. 3 ml n Us e Relativ e RT : N o Int . Type : Valle y Retentio n Time : 16- 2 ml n . Area : 17953 2 count s fr'*" * Height : 2.93e4'00 4 cp s ^ Star tTime : 16. 1 ml n %.... . En d Time : ' 16. 3 ml n | '- ^ 

## ~ 1,2* 4 1.0* 4 6000. 0 6000. 0 4000. 0 2000. 0
14. 0 14. 5 15. 0 1 SampleName:-k110802a03 rSampl e ID:-LCS-110726-> * »'i*:i(110802a.vrin " 1 Pea k Name : 'PFOS ' Mass<e8} : "499.OOCV99.000 Da.499.000/80.00 0 Da.499.00tV130.00 0 Da " 1 Comment : "20pp b LCS " Annotatton : - Sampl e Index : 3 2 Sampl e Typ.e : Q C Concentration : 19. 9 ng/m L Calculate d Conc : 21. 1 ng/m L3.4* 6 Acq . Date : 8/3/201 1 Acq . Time : 1:54:5 B A M g^je e Modified : N o Proc . Algorithm : Specif yParameter s - M Q H I 3.0e 6 Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 mi n -.8* 6 Peak-Split
. Factor : 2 Repor t Larges tPeak : Ye s Min . Pea k

Height : 0-0 0 cp s 2.6*6 - Mln . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 2.4* 6 R TWindow : 30. 0 ae c Expecte d RT : 16. 3 ml n Us e Relativ e RT : N o 2,2*6 - Int . Type : Valle y-Q^
. Retentio n Time : 16. 2 mi n - *•'" " Area : 2257028 4 count s g Height : 3.49e-f0O 6 cp s ^ 1,e«6 - Star tTime : 16. 1 ml n % En d Time : 16. 5 ml n £^g^ . 1.4* 6 1.2* 6 1.0*6 - 8.0* 5 6.0*5 - 4.0*5 - Z0*5 - 0. 0 ' ••1 1 ••< 14. 0 14, 5 15. 0 16.1 9

1 .,165 6 1 . 1 

# ll/t"'' ' 15. 5 16. 0 16. 5 17. 0 17 5 18. 0 18 5

16.1 8



# I

15 5 16. 0 16 5 17. 0 17. 5 18. 0 18 5 1 P*a k Nam* : "PFOS " Ma«9(*s) : "499.000/99.00 0 Da,499.DOO/80.00 0 Da.499.000/130.00 0 Da " •Commant-2pp b LCS " Annotatton: - Sampl e Index : 2 9 Sampl e Type : <y : Concentration : 1.9 9 ng/m L Calculate d Conc : 2.1 5 ng/m L,7 5 Acq . Pate : 6/3/201 1 * Acq . Time : 12:51:4 5 A M 2.6* 5 Modified : N o ^^ ^ Proc . Algorithm : Specif yParameter s - M Q II I 2.4* 5 Kol« e Percentage : 5 0 Base . Sub
. Window : 1.0 0 mi n 2.3* 5 Peak-Split
. Factor : 2 2.2* 5 Repor tLarges t Peak : Ye s Min . Pea k

Height : 0.0 0 cp s 2.1* 5 Min . Poa h Width : 0.0 0 se c2.0* 5 Smoothin g Width : 0 point s R TWindow : 30. 0 se c1.9* 5 Expecte d RT : 16. 3 mi n , g^ g Us e Relativ e RT : N o 1.7* 5 Int . Type : Valle y Retentio n Time ; 16. 2 ml n « '•°" ' Area : 176924 7 count s § •1.5* 5 Height : 2.80e-t00 5 cp s ^ Star t Time : 16. 1 ml n ' S '•* = En d Time : 16. 4 mi n | l.Je S 1,2* 5 1.1* 5 1.0* 5 g.te 4 8.0* 4 7.0e 4 6,0* 4 5,0* 4 4,0* 4 3.0* 4 2,0* 4 1.0* 4 14. 0 14 5 15. 0 1 Sampl * Nam* : "kll0802a03( rSampl * ID:'LCS-110726-10 " |:|le:'k1l6802a,wtf r I Pea k Name : "PFOS - Haea(es) : "499.000/99.00 0 Da,499.000fflO.OOODa,499.00a/130,00 0 Da " •Commen t "0.2pp b LC S EC P Annotatton: " Sampl e Index : 3 9 Sampl e Type : Q C Concentration : 0.19 8 ng/m L Calculate d Conc : 0.25 2 na/m L Acq . Date : 8/3/201 1 '-^" ^ Acq . Time : 4:22:4 9 A M 1.7e 4 Modified : Ye s Proc . Algorithm : Specif yParameter s - M Q II I . yj^ ^ Nois e Percentage : 5 0 Base . Sub
. Window : 1.0 0 ml n Peak-Split
. Factor : 2 '•'• * Repor tLarges t Peak : Ye s Mln . Pea k

Height : 0.0 0 cp s 1.4*4 - Kin . Pea k

Width : 0.0 0 se c Smoothin g Width : 0 point s 13,4 . R TWindow : 30. 0 se c Expecte d RT : 16. 3 ml n , . , Us e Relativ e RT : N o ^^ ^ Int . Type : Manua l 1.1*4 - Retentio n Time : 16. 2 ml n _ Area : 18460 9 count s % 10*4 - Height : 1.86e-f00 4 cp s ^ Star t Time : 15. 8 mi n 1 TM«n . En d Time : 16. 3 ml n | «»°- ° 8000, 0 7000.0 - 6000,0 ' S000.0 - 4000.0 - 3000.0 - 2000.0 - 1000.0 - 14. 0 14 5 15. 0 16,2 1



# _ f

15 5 16. 0 16 5 17. 0 17 5

Tlm*.ml n

16.1 7

16.04. , 1 

# •

^ H 16.5 1 ^ B ,Ll657 

# •1 18. 0 18 5

,.,,^,.iMl^^^ l \|fcifiYi^i^iitf^')'tiiW'^*^*it^iinht>j^ i 1 1 15 5 16. 0 16 5 17. 0 17 5 18. 0 18 5 Dat aprinte db y ST

W Printin g Time : 12:15:3 9 P M Printin g Date : Tuesday , Septembe r 06 , 201 1 Pag e4 o f 5 Pag e 9 8 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS
. an d PFO S i n Wate rSample s FSIA-Jun e 201 1 *ETS-Kir k 

### Result s Name : kll0802a.rd b 

# I Sampl e Name : 1(1106028041 * Sampl e ID : -LCS-110726-i rFUe : 'kll0802a.Mi r Pea k Name : 'PFOS ' Mas3<e*) : -499.OOCV99.000 Da,499.00Q/80XX) 0 Oa.499,000^130.00 0 Da ' Comment : *2pp b LC S ECF * Annotation : ~ Sampl eIndex : 4 2 Sampl eType : Q C Concentration : 1.9 8 ng/m L Calculate dConc : 2.2 9 ng/m L Acq . Date : 8/3/201 1 Acq . Time : 5:26:1 1 A M
Per < Window : Peak-Split . Factor : Repor t Larges t

Pea) i Min . Pea k

Height : Min . Pea k

Width : Smoothin g Width : R TWindow : rlthm : Specif y Pat ;

ntage i 1.7e 5 1.6* 5 1.5* 5 1.4e S 1.3e 5 1.2e 5 1.1* 5 1.0* 5

6.0* 4 5.0* 4

14. 0 14 5 15. 0 15 5 16. 0 16 5 17, 0 17 5 18. 0 18 5 

# I Sampl e Name:-1(110802*072 ' Sampl e ID;-GLP1(U>l-02-18-133 " File : 1(110802a.wi r Pea k Nam* : 'PFOS ' Ma3s(es) : '499.00099.00 0 Oa,499.000/80.00 0 Da.499.000/130.00 0 Da Comment : "DA LGW130LR B 110630 " Annotatlon: - 

# I Sampl*Nam*:-V110802a044 - Sampl*ID:'LCS-110726-16 - Fil*:'k110802a,wH r Pea k Name : 'PFOS ^

Maa3(*a} : '499.000/99.00 0 D8,499.000/80iXX ) Da.499.00Q/130.00 0 Oa ' Comnwn t '20pp b LC S ECF " Annotation ; ~ 19. 8 r 23. 0 r 8/3/201 1 6:29:3 0 A

M Modified : Proc . Algorithm : Nois ePercentag ' Base . Sub . Kind i Peak-Split . Factor : 2 Repor t Larges t

Peak : Y Min . Pea k

Height : 0 Min . Pea k

Width : 0 Smoothin g Width : 0 R TWindow : 30. 0 Expecte dRT : 16. 3 Specif y Parameter ! 5 0

- : 1.0 0 mi n

•RT : Int . Type : Retentio n T Height : Star t Time : En d Time :

15. 0 15 5 16. 0 16 5 17. 0 17 5 18. 0 18 5

Sampl eType : Unk i 

# ( SampleName:*k110a02a073 ' Sampl*ID:'GLPl001-02-1 1 P*a k Name : 'PFOS ' Maa3{*a) : '499.000/99.00 0 Da.499.0 r Comment : "DA LGW133 S R B 11063ff ' Annotatkin : " " Sampl eIndex : 7 4 N/ R Acq . Date : Acq . Time : Modified : Proc . Algo r Nois ePerc e thm : 8/3/201 1 4:21:0 5 P

M Ye s Specif y P a 5 0 Base . Sub . window : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t

Peak : Ye s Mln . Pea k

H « igh t Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dR

T Us eRelativ e int . Type : Retentio nT Area : Height : Star t Time : En dTime : RT : 0.0 0 0.0 0 0 30. 0 16. 3 n No Manua l 16. 2 1 706 7 co u .200*00 3 c 15. 9 1 16. 2 n Sampl eType : Concentration : Calculate dConc : Acq . Time : Modified : Proc . Algorithm : Base . Sub . Wind o Unknow n N/ A 0.047 8 e/3/201 1 4:42:1 6 P

M Ye s Specif y P a 5 0 : 1.0 0 Peak-Split . Factor : 2 Repor t Larges t

P Mln . Pea k

Heigh t Mln . Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : Us eRelativ eRT : Int . Type : Retentio nTime : Atea : Height : Star t Time : En dTime : a k : Ye s 0.0 0 0.0 0 0 30. 0 3 16. 3 n N o Manua l 16. 1 R 984 7 cou r .08e+00 4 c 15. 9 : 16. 3 I

t • t s - M

QII I 1.05e 4

1.00e 4 9500.0 0 9000.M ; 8500.00
- 8000.00
- 7500.0 0 7000.0 0 650a0 0 | . 6000,0 0 ^5500.00
- I sooaoo ^ 45oao o 4000.00 ; 3500.00 - 3000.00 - 2500.00 - 2000.0 0 1500.0 0 1000.0 0 500.00 - 0.0 0 ii4" fL^m^ifaiartiKijIIJii^ i 

### Dat aprinte db y ST

W Printin g Time : 12:15:3 9 P M Printin g Date : Tuesday , Septembe r 06 , Pag e5 o f 5 201 1 Pag e 9 9 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1


# ATTACHMEN TC : ANALYTICA LMETHO D

Pag e 10 0 o f11 3

### GLPIO-01-02 ; Interi m Repor t 1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA' - Jun e 201 1



# Mettio d Mettio d o fAnalysi s fo rtti e Determinatio n o fPerfluorinate d Compound s i n Wate r

b yLC/MS/MS ; Direc t Injectio n Analysi s Metho d Number : ETS-8-044. 0 Adoptio n Date : Upo n Signin g Effectiv e Date : Q * ^

I n f o " } Approve d By : 

# ^/^j/ a y Willia m K. Reagen , Laborator yManage rDat e

ETS-8-044. 0 Pag e 1 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direct Injectio n Analysi s Pagel0lofll 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1



# 1 Scop e an d Applicatio n Thi s metho d i s t o b e use d t o quantif yPerfluorobutanoi cAci d (PFBA) , Perfluoropentanoi cAci d (PFPeA) , Perfluorohexanoi cAci d (PFHA) , Perfluoroheptanoi cAci d (PFHpA) , Perfluorooctanoi cAci d (PFOA) , Perfluorononanoi cAci d (PFNA) , Perfluorodecanoi cAci d (PFDA) , Perfluoroundecanoi cAci d (PFUnA) , Perfluorododecanoi cAci d (PFDoA) , Perfluorobutanesulfonat e (PFBS) , Perfluorohexanesulfonate (PFHS) , an d Perfluorooctanesulfonat e (PFOS ) byHig h Perfonnance Liqui d Chromatograph ycouple d t o a tande m Mass Spectrometri cDetecto r(LC/MS/MS ) i n clea n wate rsamples . Wate rsample s containin g heav y particulat e ma

yrequire preparatio n b

ya n alternat e metho d suc h as ETS-8-15 4 "Determinatio n of Perfluorinate d Acids , Alcohols , Amides , an d Sulfonate s I n Wate rB ySoli d Phas e Extractio n an d Hig h Performance Liqui d Chromatography/Mas s Spectrometry"
. Thi s metho d i s considere d a performance-base d method . Data i s considere d acceptabl e a s lon g as th e define d Q C elements are satisfied
. Sampl e collectio n i s no

t covere d unde rthi s analytica l procedure . 2 Metho d Summar y Clea n aqueou s sample s are analyze d b ydirec tinjectio n usin g LC/MS/MS
. Sample s containin g heav y particulat e ma

yno t b e suitabl e fo ranalysi s b ythi s method . Sample s containin g suspende d particulat e shoul d b e centrifug e prio rt o removin g a sampl e aliquot , o rfiltered
. Thi s i s a performance-base d method . Metho d accurac yi s detemnined fo reac h sampl e setusin g multipl e laborator ycontro l spike s at multipl e concentrations . Thi s metho d als o require s thatth e precisio n an d accurac yfo reac h sampl e b e determine d usin g fiel d matri xspike s t o verif ythatth e metho d i s applicabl e t o eac h sampl e matrix . Sampl e results fo rspike s outsid e o f70 % t o 130% , ma yb e flagge d a s suc h (wit h expande d accurac

ystatements) , o rwil l no

tb e reporte d du e t o non-complian tqualit ycontro l samples . Fortificatio n level s fo rfiel d matri xspike s an d fo rlaborator ymatri xspike s shoul d b e a tleast50 % ofth e endogenou s leve l an d les s tha n 1 0 time s th e endogenou s leve l t o b e use d t o determin e th e statemen tof accurac yfo ranalytica l results . 

# 3 Definition s 3. 1 Calibratio n Standar d Asolutio n prepare d b

yspikin g a know n volum e o fth e Worthin g Standar d (WS ) into a predetermine d amoun t o fAST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , an d analyze d accordin g t o thi s method . Calibratio n standard s are use d t o calibrat e th e instmmen trespons e wit h respec tt o analyt e concentration . 3. 2 Laborator yDuplicat e Sampl e (LDS , o rLa b Dup ) Alaborator

yduplicat e sampl e i s a separat e aliquoto fa sampl e take n i n th e analytica l laborator ythati s analyze d separatel ywit h identica l procedures . Analysi s o fLDS s compare d t o thatofth e firs taliquotgive a measur e ofth e precisio n associate d wit h laborator yprocedures , bu tno

twit h sampl e collection , preservation , o rstorag e procedures . 

# 3. 3 Fiel d Blan k

(FB)/Tri p Blan k AST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , place d i n a sampl e containe ri n th e laborator y an d treate d a s a sampl e i n al l respects , includin g exposure t o samplin g sit e conditions , storage , preservatio n an d al l analytica l procedures . Th e purpos e ofth e F B i s t o determin e i ftes t substance s o r othe rinterference s are presen t i n th e field environment . Thi s sampl e i s als o refen-ed t o as a Tri p Blank . ETS-8-044. 0 Pag e 2 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direct Injectio n Analysi s Pag e 102 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1


# 3. 4 Fiel d Duplicat e Sampl e (FDS , Fiel d Dup ) Asampl e collecte d i n duplicat e a t th e sam e tim e fro m th e sam e locatio n as th e sample . Th e FD S i s handle d unde ridentica l circumstance s an d treate d exactl yth e sam e throughou tfield an d laborator y procedures . Analysi s ofth e FD S compare d t o thato fth e first sampl e give s a measur e ofth e precisio n associate d wit h sampl e collection , preservatio n an d storage , a s wel l a s wit h laborator yprocedures . 

# 3. 5 Fiel d Matri xSpik e (FMS ) Asampl e t o whic h know n quantitie s ofth e targe tanalyte s are adde d t o th e sampl e bottl e i n th e laborator y befor e th e bottle s are sen t t o th e fiel d fo rcollectio n ofaqueou s samples . Aknown , specifi cvolum e of sampl e must b e adde d t o th e sampl e containe rwithou trinsing. Thi s ma yb e accomplishe d b

ymakin g a "fil l t o thi s level " lin e o n th e outsid e ofth e sampl e container . Th e FM S shoul d b e spike d betwee n approximatel y50 % an d 1 0 time s th e expecte d analyt e concentratio n i n th e sample . I fth e expecte d rang e ofanalyt e concentration s i s unknown , multipl e spike s atvaryin g level s ma yb e prepare d t o increas e th e likelihoo d that a spike a

t a n appropriat e leve l i s made . Th e FM S i s analyze d t o ascertai n i fan ymatri x effects , interferences , o rstabilit yissue s ma

ycomplicat e th e interpretatio n ofth e sampl e analysis . 3. 6 Tri p Blan k

Spik e (Fiel d Spik e Contro l Sample , FSCS ) A n aliquot ofAST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , t o whic h know n quantitie s o fth e targe t analyte s are adde d i n th e laborator yprio rt o th e shipmen to fth e collectio n bottles . Th e FSC S i s extracte d an d analyze d exactl ylik e a stud ysampl e t o hel p detemnin e i fth e metho d i s i n contro l an d whethe ra los s ofanalyt e coul d b e attribute d t o holdin g time , sampl e storag e and/o rshipmen tissues . A lo wan d hig h FSC S are appropriat e whe n expecte d sampl e concentration s ar e no tknow n o rma

yvary . At least on e separate , un-spike d sampl e mustb e take n atth e sam e tim e an d place as eac h FMS
. 

# 3. 7 Laborator yContro l Sampl e (LCS ) A n aliquot ofcontro l matri xt o whic h know n quantitie s o fth e targe tanalyte s are adde d i n th e laborator ya t th e tim e ofsampl e extraction . At leasttwo level s are included , on e generall yatth e lo wen d ofth e calibratio n curve an d on e nea rth e mi d t o uppe rrang e o fth e curve . Th e LCS s are extracte d an d analyze d exactl ylik e a laborator

ysampl e t o determin e whethe rth e metho d i s i n control . LCS s shoul d b e prepare d eac h da ysample s are extracted . 

# 3. 8 Laborator yMatri xSpik e (LMS ) Alaborator

ymatri xspike i s a n aliquot o fa sampl e t o whic h know n quantitie s oftargetanalyte s are adde d i n th e laboratory . Th e LM S i s analyze d exactl ylik e a laborator

ysampl e t o detemiin e whethe rth e sampl e matri xcontribute s bias t o th e analytica l results . Th e endogenou s concentration s o fth e analyte s i n th e sampl e matri xmust b e determine d i n a separate aliquotan d th e measure d value s i n th e LM S correcte d fo rthes e concentrations . LMS s are optiona l fo ranalysi s ofaqueou s samples . 

# 3. 9 Laborator ySampl e Aportio n o raliquot ofa sampl e receive d fro m th e field fo rtesting . 

# 3.1 0 Limi t o fQuantitatio n (LOQ ) Th e lowe rlimi t o fquantitatio n (LLOQ ) fo ra dataseti s th e lowestconcentratio n thatca n b e reliabl y quantitate d withi n th e specifie d limits o fprecisio n an d accurac ydurin g routin e operatin g conditions . T o simplif ydat a reporting , th e LLOQ i s generall yselecte d as th e lowestnon-zer o standar d i n th e calibratio n curve that meets metho d criteria . Sampl e LLOQ s are matrix-dependent . Th e uppe rlimi t ofquantitatio n (ULOQ ) fo ra dataseti s th e highestconcentratio n thatca n b e reliabl y quantitate d withi n th e specifie d limits ofprecisio n an d accurac ydurin g routin e operatin g conditions . Th e highest standar

d i n th e calibratio n curve thatmeets metho d criteri a i s define d as th e ULOQ
. ETS-8-044. 0 Pag e 3 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direct Injectio n Analysi s Pag e 10 3 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSI A- Jun e 201 1


# 3.1 1 Metho d Blan k A n aliquot ofcontro l matri xthat i s treate d exactl ylik e a laborator

ysampl e includin g exposure t o al l glassware , equipment , solvents , an d reagents thatare use d wit h othe rlaborator ysamples . Th e metho d blan k i s use d t o determin e i ftes t substance s o rothe rinterference s are presen ti n th e laborator y environment , th e reagents , o rth e apparatus . 

# 3.1 2 Sampl e Asampl e i s a n aliquot remove d fro m a large rquantit yo fmateria l intende d t o represen tth e origina l source material . 

# 3.1 3 Stoc k

Standar d Solutio n (SSS ) Aconcentrate d solutio n ofa single-analyt e prepare d i n th e laborator ywit h a n assaye d reference compound . 

# 3.1 4 Surrogat e Acompoun d simila ri n chemica l compositio n an d behavio rt o th e targe tanalyte(s) , bu ti s notnormall y foun d i n th e sample(s)
. Asurrogat e compoun d i s typicall ya targe tanalyt e wit h a tleaston e ato m containin g a n isotopically-labeie d substitution . I fused , sun-ogate(s) are adde d t o al l sample s an d qualit y contro l samples . Sun'ogate(s ) are adde d t o quantitativel yevaluat e th e entire analytica l procedur e includin g sampl e collection , preparation , an d analysis . Inclusio n ofa sun-ogat e analyt e i s a n optiona l qualit ycontro l measur

e an d i s NO

Trequired . 

# 3.1 5 Workin g Standar d (WS ) Asolutio n ofsevera l analyte s prepare d i n th e laborator yfro m SSS s an d dilute d as neede d t o prepare calibratio n standard s an d othe rrequire d analyt e solutions . 

# 4 Warning s an d Caution s 4. 1 Healt h an d Safet y Th e acut e an d chroni ctoxicit yofth e standard s fo rthi s metho d have no tbee n precisel ydetermined ; however , eac h shoul d b e treate d as a potentia l healt h hazard . Th e analystshoul d wea rgloves , a la b coat , an d safet

yglasse s t o preven t exposure t o chemical s thatmigh tb e present . Th e laborator

yi s responsibl e fo rmaintainin g a safe wori cenvironmen tan d a cun-entawarenes s o floca l regulation s regardin g th e handlin g ofth e chemical s use d i n thi s method . Areference file ofmateria l safet y dat a sheets (MSDS ) shoul d b e availabl e t o al l personne l involve d i n thes e analyses . 

# 4. 2 Caution s Th e analyst must b e familia rwit h th e laborator yequipmen tan d potentia l hazard s including , bu tno tlimite d to , th e us e o fsolvents , pressurize d ga s an d solven tlines , hig h voltage , an d vacuu m systems . Refe rt o th e appropriat e equipmen t procedur e o roperato rmanua l fo radditiona l infonnatio n an d cautions . 

# 5 Interference s Durin g sampl e preparatio n an d analysis , majo rpotentia l contaminan tsource s are reagents an d glassware . Al l material s use d i n th e analyse s shal l b e demonstrate d t o b e fre e fro m interference s unde r condition s ofanalysi s b

yrunnin g metho d blanks .

ETS-8-044. 0 Pag e 4 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direct Injectio n Analysi s Pag e 10 4 o f11 3

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## Parts an d supplie s that contai n Teflon® shoul d b e avoide d o rminimize d du e t o th e possibilit yof interference and/o rcontamination . Thes e ma yinclude , bu tare no

tlimite d to : was h bottles . Teflon ® line d caps , autovia l caps , HPLC parts , etc . Th e us e ofdisposabl e micropipette s o rpipette s t o aliquotstandar d solution s i s recommende d t o mak e calibratio n standard s an d matri xspikes . 

# 6 Instrumentation , Supplies , an d Equipmen t 6. 1 Instrumentatio n an d Equipmen t Ahig h perfonnance liqui d chromatograp h capabl e ofpumpin g u p t o tw o solvents an d equippe d wit h a variabl e volum e injecto rcapabl e o finjectin g 5-10 0 |J Lconnecte d t o a tande m Mass Spectromete r (LC/MS/MS)
. I Analytica l balance capabl e ofreadin g t o 0.000 1 g Adevice t o collec

t ra wdat a fo rpea k

integratio n an d quantitatio n 15-m Lan d 50-m Ldisposabl e polypropylen e centrifug e tubes . Gas tigh t syringes , 25|jL , 50|JL , 

## lOOpL , 250|jL , 500^JL, lOOOpL
. 1 m Lplasti cHPLC autovial . Disposabl e pipettes , polypropylen e o rglas s as appropriat e Centrifug e capabl e ofspinnin g 15-m Lan d 50-m Lpolypropylen e tube s a t300 0 rpm . 

# 6. 2 Chromatographi cSyste m Guar d Column : Pris m RP , 4. 6 m m x5 0 mm , 5 p m Analytica l Column : Betasi l C I 8,4. 6 m m x10 0 mm , 5 ij m Temperature : 10° C Mobil e Phas e (A) : 2 m M Ammoniu m Acetat e i n Wate r Mobil e Phas e (B) : Methano l Gradien t Program :

Tim e (min ) 0. 0 0. 5 11. 0 13. 5 13. 6 17. 0 % A

9 7 9 7 5 5 9 7 9 7 % B

3 3 9 5 9 5 3 3 FlowRat e

(mL/min ) 1. 0 1. 0 1. 0 1. 0 1. 0 1. 0 Injectio n Volume : 10 0 p L Quantitation : Pea k

Are a - quadrati ccurve fit, 1/ xweighted . Ru n Time : ~ 1 7 minutes . Th e previou s informatio n i s intende d a s a guide ; alternat e condition s an d equipmen tma yb e use d provide d tha t dat a qualityobjective s ar e met . ETS-8-044. 0 Pag e 5 o f1 1 Metho d ofAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direct Injectio n Analysi s Pag e 10 5 o f11 3

### GLP10-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1


# 6. 3 MS/M S Syste m 6.3. 1 Mode : Electrospra yNegativ e on , MR M mode , monitorin g th e followin g transitions : Analyt e PFBA PFPeA PFH A PFHpA PFOA PFN A PFDA PFUn A PFDoA PFB S PFH S PFO S Transitio n Monitore d 213^16 9 263-^21 9 31 3 ^26 9 an d 313-^11 9 36 3 - ^ 319 , 36 3 - ^

16 9 an d 36 3 - > 11 9 41 3 ^ 369 , 41 3 ^

21 9 an d 41 3 ^

16 9 46 3 - ^ 419 , 46 3 - ^

16 9 an d 46 3 ^

21 9 51 3 ^ 469 , 51 3 ^

21 9 an d 51 3 ^

26 9 56 3 - » 519 , 56 3 - ^

26 9 an d 56 3 - > 21 9 61 3 ^ 569 , 61 3 - ^

16 9 an d 61 3 ^

31 9 29 9 - ^ 8 0 an d 29 9 - ^

9 9 39 9 - ^ 8 0 an d 39 9 - > 9 9 49 9 ^ 80 , 49 9 - ^

9 9 an d 49 9 - ^

13 0 Multipl e transition s fo rmonitorin g th e analyte s i s a n option , a s summin g multipl e transition s ma yprovid e quantitatio n o fisomers tha t more closel ymatche s NM R dat a an d ma yhave th e adde d benefi to f increase d sensitivity . Th e us e o fon e daughte rio n i s acceptabl e i fmetho d sensitivityi s achieved , provide d tha t retentio n tim e criteri a ar e me tt o assur e adequat e specificity
. Th e previou s informatio n i s intende d as a guide , alternat e instmments an d equipmen tma yb e used . 

# 7 Reagent s an d Standard s 7. 1 Chemical s Water - Milli-Q , HPLC grade , o rothe rsuitabl yappropriat e source s Methano l - HPLC grad e Ammoniu m Acetat e - A.C.S
. Reagen tGrad e 

# 7. 2 Standard s Perfluorobutanoi cAci d (PFBA- C4 acid) ; Oakwoo d Products , In c Perfluoropentanoi cAci d (PFPeA- C 5 acid , als o know n as NFPA , nonafluoropentanoi cacid) ; Alf a Aesa r Perfluorohexanoi cAci d (PFHA-C 6 acid) ; Oakwoo d Products , In c Perfluoroheptanoi cAci d (PFHpA- C7
. acid , als o know n as TDHA , tridecafluoroheptanoi cacid) ; Oakwoo d Products , In c Perfluorooctanoi cAci d (PFOA- C 8 acid) ; 3 M Perfluorononanoi cAci d (PFNA-C 9 acid) ; Oakwoo d Products , In c Perfluorodecanoi cAci d (PFDA-CI O acid) ; Oakwoo d Products , In c Perfluoroundecanoi cAci d (PFUnA-C1 1 acid) ; Oakwoo d Products , In c Perfluorododecanoi cAci d (PFDoA-C I 2 acid) ; Oakwoo d Products , In c Perfluorobutanesulfonat e (PFB S - C 4 sulfonate) ; 3 M Perfluorohexanesulfonate (PFH S - C 6 sulfonate) ; 3 M Perfluorooctanesulfonat e (PFO S - C 8 sulfonate) ; 3 M ETS-8-044. 0 Pag e 6 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; DirectInjectio n Analysi s Pag e 10 6 o f11 3

### GLP10-01-Q2 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Th e previou s informatio n i s intende d a s a guide . Reagent s an d standard s fro m alternat e source s ma yb e used . 7. 3 Reagen t Preparatio n 2 m M Ammoniu m acetat e solutio n (Analysis)—Weig h 0. 3 g ofAmmoniu m acetat e an d dissolve i n 2. 0 Lofreagen t water . Note : Alternative volume s ma yb e prepare d a s lon g as th e ratio s ofth e solven tt o solut e ratio s are maintained . 

# 7. 4 Stoc k

Standar d Solutio n (SSS ) an d Workin g Standar d Solutio n Preparatio n Th e followin g standar

d preparatio n procedure serve s a s a n example . Weighe d amounts an d final volume s ma yb e change d t o sui t th e need s ofa particula rstudy . Fo rexample , p Lvolume s ma yb e spike d into volumetri cflasks whe n dilutin g stoc k

solution s t o appropriat e levels . 10 0 |jg/m Ltarge t analyt e SSSs—^Weig h ou t1 0 m g o fanalytica l standar d (correcte d fo rpercen tsal tan d purit yan d dilut e t o 10Om Lwit h methano l o rothe rsuitabl e solvent , i n a 10Om Lvolumetri cflask. Transfe rt o a 125m LLDP E bottl e o rothe rsuitabl e container . Prepar e a separat e solutio n fo reac h analyte . Expiratio n date s an d storag e condition s ofstoc k solution s shoul d b e assigne d i n accordance wit h laborator ystandar d operatin g procedure . A n exampl e ofpurit yan d sal tcorrectio n i s give n belowfo rPFOS
. ^ . , . molecula rweigh to fanio n sal t correctio n facto r= = moclecula rweigh to fsal t

4Q d 

## PFO S (K- ^ )sal t correctio n facto r= ^^ ^ = 0.927 5 53 8 1 0 m g CsFiySOs'K

* wit h purit

y90 % = 8.35m g C8F17SO3 " (1 0 mg*0.90*0.9275=8.3 5 mg ) 5 |jg/m L(500 0 ng/mL ) mixe d workin g standard—Ad d 0.5m Leac h ofth e lOOpg/m LSSS s t o a lOm Lvolumetri c flask an d brin g u p t o volum e wit h solvent . 25 0 ng/m Lmixe d workin g standard—Ad d 1.25m Lofth e 5 pg/m L-mixe d wori<in g standar d solutio n t o a 25m L volumetri cflask

an d brin g u p t o volum e wit h solvent . 12 5 ng/m Lmixe d standard—Ad d 625p Lo fth e 5 pg/mL-mixe d woricin g standar d solutio n t o a 25m Lvolumetri c flask an d brin g u p t o volum e wit h solvent . Storag e Conditions—Stor e al l SSS s an d wori<in g standard s i n accordance wit h laborator ystandar d operatin g procedur e o ri n a refrigerato rat 4°±2° C fo ra maximu m perio d of6 month s fro m th e dat e ofpreparation .

ETS-8-044. 0 Pag e 7 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; DirectInjectio n Analysi s Pag e 10 7 of11 3

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# 7. 5 Calibratio n Standard s Usin g th e worthin g standard s describe d above , prepare calibratio n solution s i n AST M Typ e I water , HPLC water , o r othe rsuitabl e water , usin g th e followin g tabl e as a guideline . Note : Volume s ofwate ran d woridn g standard s ma y b e adjuste d t o meet th e dat a qualit yobjective s addresse d i n th e genera l projec toutline . Calibratio n level s othe r tha n thos e liste d belowca n b e prepare d as needed . Concentratio n o fWS , ng/m L 12 5 12 5 12 5 12 5 25 0 25 0 25 0 25 0 500 0 500 0 500 0 Volum e o fWS , fd
.

1 0 1 5 2 0 3 0 2 0 5 0 10 0 20 0 2 5 5 0 10 0 Fina l Volum e o f

Calibratio n Standar d (m L o fAST M Typ e 1 Water , o r othe rsuitabl e water )

5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 Fina l Concentratio n o f

Calibratio n Standard , ng/m L (ppb ) i n ASn H Typ e 1 Water , o rothe rsuitabl e wate r 0.02 5 0.037 5 0.05 0 0.07 5 0.10 0 0.25 0 0.50 0 1.00 2.5 0 5.0 0 10. 0 

## 8 Sampl e Handlin g 8. 1 Wate rSampl e Preparatio n Thi s metho d i s applicabl e t o clea n wate rsamples . Sample s containin g heav yparticulat e ma

ynotb e suitabl e fo r analysi s b

ythi s method . Sample s containin g suspende d particulat e shoul d b e centrifug e prio rt o removin g a sampl e aliquot , o rfiltered. Thoroughl ymi xsampl e befor e removin g a n aliquotan d placin g i n a labele d plasti cautovial . Plasti ci s preferre d ove rth e us e ofglas s autovials , t o preven tth e possibl yo ffluorochemical stickin g t o th e glass . Dilut e sample , i fnecessary , wit h AST M Typ e I , HPLC water , o rothe rsuitabl e water . Prepare metho d Q C sample s an d multipl e metho d blank s an d aliquotinto labele d plasti cautovials . Prepar e at least five metho d blanks . 9 Sampl e Analysi s - LC/MS/M S Analyz e th e standar

d curve prio rt o eac h setofsamples . Th e standar d curve ma

yb e plotte d usin g a linea rfit, weighte d 1/

xo runweighted , o rb

yquadrati cfil (

y= ax ^ - H bx+ c) , weighte d 1/

xo runweighted , usin g suitabl e software
. Th e calibratio n curve s ma yinclud e bu tshoul d no

tb e force d throug h zero . Th e mathematica l metho d use d t o calculat e th e calibratio n curve shoul d b e applie d consistentl ythroughou ta study . An ychang e shoul d b e thoroughl ydocumente d i n th e ra wdata . Hig h and/o rlo

wpoints ma

yb e exclude d fro m th e calibratio n curve s t o provid e a bette rfitove rth e rang e appropriat e t o th e data o rbecaus e theydi d no tmeetth e pre-detemnined acceptance criteria . Low-leve l curve points shoul d als o b e exclude d i fthei rare a counts are no tatleasttwic e thatofth e metho d and/o rsolven tblanks . Th e coefficien t ofdetenninatio n (r^ ) valu e fo rth e calibratio n curve mustb e greate rtha n o requa l t o 0.990 . Eac h poin t i n th e curve must b e withi n ±25 % o fth e theoretica l concentratio n wit h th e exceptio n o fth e LLOQ , whic h ma y ETS-8-044. 0 Pag e 8 o f1 1 Metho d o fAnalysi s fo rth e Detenninatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; DirectInjectio n Analysi s Pag e 10 8 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## b e withi n ±30% . Justificatio n fo rexclusio n ofcalibratio n curve points wil l b e note d i n th e ra wdata . Aminimu m o f6 points wil l b e use d t o constmc t th e calibratio n curve . I fth e calibratio n curve doe s not meet acceptance criteria , perfom n routin e maintenance o rprepare a newstandar d curve (i fnecessary) an d reanalyze . Continuin g calibratio n verification s (CCV) are analyze d t o verif yth e accurac

yofth e calibratio n curve . Analyz e a mid-rang e calibratio n standard , on e o fth e sam e standard s use d t o constnjctth e calibratio n curve , a ta minimu m afte rever ytent h sample , no

t includin g solven tblanks , wit h a minimu m ofon e pe rsampl e set . Calibratio n verificatio n injection s must b e withi n ±25 % t o b e considere d acceptable . Th e calibratio n curve an d th e lastpassin g CCVwil l the n bracket acceptabl e samples . Multipl e CCVlevel s ma yb e used . Sample s containin g analyte s thatare quantitate d above th e concentratio n o fth e higheststandar

d i n th e curve shoul d b e furthe rdilute d an d reanalyzed . 

# 1 0 Qualit yContro l 10. 1 Dat a Qualit yObjective s Thi s metho d an d require d qualit ycontro l sample s i s designe d t o generat e data accurat e t o ±30 % wit h a targete d LOQ of0.02 5 ng/mL
. An ydeviation s fro m th e qualit ycontro l measure s spelle d ou tbelowwil l b e documente d i n th e ra wdata an d footnote d i n th e final report . 

# 10. 2 Metho d Blank s Metho d blank

s must b e prepare d wit h eac h analysi s batch . Atleastfive metho d blank s mustb e prepared . Metho d blank

s ma yb e injecte d multipl e times , bu tn o mor e tha n 3 injection s shoul d b e remove d firom a singl e metho d blank . At a minimum , metho d blank s are analyze d prio rt o instmmen tcalibration , prio rt o th e analysi s of CCVsamples , an d a

t th e en d o fth e analytica l run . Th e mea n are a coun t fo reac h analyt e i n th e metho d blank s mustb e les s tha n 50 % ofth e are a coun tofth e LOQ standard . Th e standar

d deviatio n ofth e are a counts ofthes e metho d blank s shoul d b e calculate d an d reported . I f th e mea n are a counts o fth e metho d blank s excee d 50 % ofth e LOQ standard , the n th e LOQ mustb e raise d t o th e first standar

d leve l i n th e curve that meets criteria , o raltematively , th e metho d blank s mustb e evaluate d statisticall yt o detennin e outliers , o rtechnica l justificatio n t o eliminat e on e o rmor e results shoul d b e made . 

# 10. 3 Sampl e Replicate s Sample s duplicate s are collecte d i n th e field. Th e relafive percen tdifference , RPD , shoul d b e reported . RP D results greate rtha n 20 % wil l b e flagged i n th e report , bu twil l no tb e exclude d fi'om reporting . Th e requiremen tfo r replicate s exclude s field blanks . 

# 10. 4 Surrogat e Spike s Surrogate spike s are no

t require d bu t ma

yb e use d o n projec

tspecifi crequirements . 

# 10. 5 La b Contro l Sampl e Triplicat e la b contro l spike s a t a minimu m oftwo differen tconcentration s are t o b e prepare d wit h eac h preparatio n batch . Lo wla b contro l spike s shoul d b e prepare d atconcentration s i n th e rang e o ffive t o te n time s highe rtha n th e targete d LOQ an d hig h la b contro l spike s shoul d b e prepare d a tconcentration s nea rth e mid-poin to fth e curve . Th e relative standar

d deviatio n o fth e contro l spike s evaluate d independentl ya teac h concentratio n leve l mustb e les s tha n o requa l t o 20 % an d th e averag e recover ymustb e 80-120% . I fth e above criteri a are no tmet , th e entire set ofsample s shoul d b e re-injecte d o rre-prepare d as appropriate .

ETS-8-044. 0 Pag e 9 o f1 1 Metho d o fAnalysi s fo rth e Detenninatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; DirectInjectio n Analysi s Pag e 10 9 o f11 3

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# 10. 6 Fiel d Matri xSpike s / La b Matri xSpike s Recoverie s o ffield matri xspike s an d laborator ymatri xspike s are anticipate d t o b e betwee n 70 % an d 130 % ofth e fortifie d levels . Sampl e results fo rspike s outsid e of70 % t o 130% , ma yb e fiagged as suc h (wit h expande d accurac ystatements) , o rwil l no

t b e reporte d du e t o non-complian tqualit ycontro l samples . Th e targete d fortificatio n level s shoul d b e a tleast50 % o fth e endogenou s leve l an d les s tha n 1 0 time s th e endogenou s leve l t o b e use d withou t justificatio n t o detennin e th e statemen tofaccurac yfo ranalytica l results . Th e averag e ofth e sampl e an d th e field duplicat e shoul d b e use d t o calculat e th e recovery . 1 1 Dat a Analysi s an d Calculation s Us e th e followin g equatio n t o calculat e th e amoun tofanalyt e foun d (i n ng/mL , base d o n pea k area) usin g th e standar d curve (linea rregressio n parameters ) generate d b ya n appropriat e software program :

. , ^ , . , , , , (Pea k Area-Intercept ) ^ ^ Analyt e foun d (ng/mL ) = -^ ^ ^-^

xD F Slop e D F = facto rb

ywhic h th e final volum e wa s diluted , i fnecessary . Fo rsample s fortifie d wit h know n amounts ofanalyt e prio rt o extraction , us e th e followin g equatio n t o calculat e th e percen t recovery .

_Tota l analyt e foun d (ng/mL ) - Averag e analyt e foun d i n sampl e (ng/mL ) Analyt e adde d (ng/mL ) 1 2 Metho d Performanc e An ymetho d perfonnance parameter s thatare no

tachieve d mustb e considere d i n th e evaluatio n ofth e data . Nonconfomnanc

e t o an yspecifie d parameter s mustb e describe d an d discusse d i fth e Technica l Manage r(non GL P study) o rStud yDirecto r(GL P study) choose s t o repor tth e data . I fcriteri a liste d i n thi s metho d perfomnanc e sectio n are no

tmet , maintenance ma

yb e perfonne d o n th e syste m an d sample s reanalyzed , o rothe raction s take n as appropriate . Documen tal l action s i n th e ra wdata . I fdata are t o b e reporte d whe n perfomnanc e criteri a hav

e no tbee n met , th e data mus tb e footnote d o n table s an d discusse d i n th e tex

t o fth e report . 

# 12. 1 Syste m Suitabilit y Syste m Suitabilit

ystandard s are no

t a require d componen tofthi s method . I frequire d b yprotoco l o rb

yth e technica l manager , a minimu m ofthre e syste m suitabilit ysample s are injecte d a

tth e beginnin g o feac h analytica l m n prio rt o th e calibratio n curve . Typicall ythes e sample s are a ta concentratio n nea rth e mi d leve l ofth e calibratio n curve an d are repeate d injection s fro m on e autosample rvial . Th e syste m suitabilit yinjection s must have are a counts wit h a n RS D o fS5% an d a retentio n tim e RS D of<2 % t o b e compliant . 12. 2 Quantitatio n Calibratio n Curve : Th e coefficien t ofdetenninafio n (r^ ) valu e fo rth e calibratio n curve mustb e greate rtha n o r equa l t o 0.990 . Eac h poin t i n th e curve mustb e withi n ±25 % ofth e theoretica l concentratio n wit h th e exceptio n of th e LLOQ , whic h ma

yb e withi n ±30% . CCVPerfonnance : Th e calibratio n standard s thatare intersperse d throughou tth e analytica l sequence are evaluate d a s continuin g calibratio n verification s i n additio n t o bein g par to fth e calibratio n curve . Th e accurac yo f eac h curve poin t must b e withi n 25 % ofth e theoretica l valu e (withi n 30 % fo rlowestcurve point)
. Sample s thatare brackete d b

yCCVs no

t meefin g thes e criteri a mustb e reanalyzed .

ETS-8-044. 0 Pag e 1 0 o f1 1 Metho d ofAnalysi s fo rth e Detenninatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; DirectInjectio n Analysi s Pag e 11 0 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s ofPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1



## Demonstratio n o fSpecificity : Specificit yi s demonstrate d b

ychromatographi cretentio n tim e (withi n 4 % of standard ) an d th e mas s spectra l respons e o funiqu e ions . 12. 3 Sensitivit y Th e targete d limi t o fquantitatio n fo ral l analyte s i s 0.02 5 ng/mL
. Th e LOQ fo ran yspecifi canalyt e ma yvar y dependin g o n th e evaluatio n ofappropriat e blank s an d th e accurac

yo fth e low-leve l calibratio n curve points . Refe r t o Sectio n 1 0 fo radditiona l details . 

# 12. 4 Accurac y Thi s metho d an d require d qualit ycontro l sample s are designe d t o generat e data thatare accurat e t o +1-30% . Sectio n 1 0 contain s additiona l informatio n regardin g th e require d accurac yoflaborator

ycontro l spikes , field matri x spike s an d laborator

ymatri xspikes . 

# 12. 5 Precisio n Sample s shoul d b e collecte d i n duplicat e i n th e field. Th e relative percen tdifference , RPD , shoul d b e reported . RP D results greate rtha n 20 % wil l b e flagged i n th e report , bu twil l notb e exclude d fro m reporting . Th e requiremen t fo rreplicate s exclude s field blank s o rrinse blanks . Sectio n 1 0 contain s additiona l infonnatio n regardin g th e require d precisio n oflaborator ycontro l spikes . 

# 1 3 Pollutio n Preventio n an d Wast e Managemen t Wast e generate d whe n perfomnin g thi s metho d wil l b e dispose d ofappropriately . Th e origina l sample s wil l b e archive d at th e 3 M Environmenta l Laborator yi n accordance wit h intema l procedures . 

# 1 4 Record s Eac h data packag e generate d fo ra stud ymustinclud e al l supportin g infonnatio n fo rreconstructio n o fth e data . Infonnatio n fo rth e dat a packag e must include , bu ti s no tlimite d t o th e followin g items : stud yo rprojec tnumber , sampl e an d standar

d pre p sheets/records , instmmen tru n lo g (instrumen tbatc h records , instrumen tacquisitio n method , summar

ypages) , instmmen t results files, chromatograms , calibrafio n curves , an d data calculations . 

# 1 5 Affecte d Document s None . 

# 1 6 Revision s Revisio n Numbe rSummar yo fChange s

ETS-8-044. 0 Pag e 1 1 of1 1 Metho d o fAnalysi s fo rth e Detenninatio n ofPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; DirectInjectio n Analysi s Pag e 11 1 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 Analysi s o fPFBS , PFHS , an d PFO S i n Wate rSample s FSIA-Jun e 201 1


# ATTACHMEN TD : DEVIATION(S )

Pag e 11 2 o f11 3

### GLPIO-01-02 ; Interi m Repor t1 8 

# RECOR D O F DEVIATI0N/N0N^e^r6iMtV"^'TM'?sr;uSi ?

/
. Identificatio n stud y/ Projec t No . GLP10-01-02-1 8 Date(s ) o fOccurrence : kll0729b , l<110802 a Documen t Number : ETS-8-044. 0 Deviatio n typ e •SO P D Equipmen t Procedur e 0 Metlio d (Chec k one ) D Protoco l D GP O n Other :

//•Descriptio n (attac h extr a page s a s needed ) 

## Metho d Requirements : 1 . LC S averag e recoverie s wntiii n ±20 % (sectio n 10.5)
. 2 . FIVI S recover

ywithi n ±30 % (sectio n 10.6)
. 3 . RP D withi n ±20 % (sectio n 10.3)
. 4 . Syste m suitabilitypea k are a RS D S5 % an d retentio n tim e RS D <2%>. 

## Actua l procedure/process : 1 . I<110729 b (interna l calibration) : Th e lo wse t ofPFO S EC F LC S sannples ha d a n averag e recover yof136 % an d th e hig h se tofEC F LC S sample s had a n averag e recover yof122% . I<110802a : Th e low , mid , an d hig h PFO S EC F LC S sample s ha d averag e recoverie s of129% , 122% , an d 122% . 2 . Th e followin g sample s ha d FM S recoverie s les s tha n 70 % fo rPFOS : 130L , 131L , 135L , 136L , 141R , 142R , an d 1443 . Sample s 133 Lan d 135 S had FM S sample s greate rtha n 150 % fo rPFOS
. 3 . Sampl e 134 Lha d a n RP D of22 % fo rPFOS
. 4 . k110729 b (externa l calibration) : Th e pea k are a RS D fo rPFO S wa s 9.3% . 

## ///
. Action s Take n (suc h a s amendmen t issued , SO P revision , etc. ) 

## Correctiv e Actio n ( D Ye s IZI No ) Reference : Acceptabilit yo fth e nonconformin g work : 1 . Th e recoverie s fo rth e non-complian t LCS s (linea r+ branched ) wil l b e flagge d i n th e fina l report . A N ofth e LCS s prepare d wit h thi s stud ywere evaluate d byETS-12-01 2 t o determin e th e metho d analytica l uncertainty . Th e analytica l uncertaintywhe n evaluate d byETS-12-01 2 wa s determine d t o b e ±28 % fo r PFOS
. However , th e analytica l uncertaintyfo rthi s stud ywil l b e se t at ±36% , base d o n th e recover yofth e lo wse toflinea r+ branche d LC S fro m ru n kl l 0729b . 2 . Th e non-complian tFM S recoverie s fo rPFO S vA Wb e flagge d i n th e fina l report . I fnecessary , th e metho d uncertaintywil l b e expande d fo rsample s wit h FM S recover yles s tha n 64% . Sample s 133 Lan d 135 3 wil l not b e reporte d fo rPFO S du e t o th e FM S recoverie s bein g outsid e th e £50 % acceptanc e criteria . 3 . Th e PFO S RP D fo rsampl e 134 Lwil l b e flagge d i n th e fina l report . 4 . Othe rQ C item s were use d t o asses s dat a accurac yfo rPFO S - calibratio n curv e poin t accuracy , curv e fi t criteria , CCVs , an d LCS , al l meetin g metho d acceptanc e criteria . Actions : D Haltin g o fWor k D Clien t Notificatio n D Wor k Recal l D Withholdin g o fRepor t 0 Other : Deviation s wil l b e note d i n fina l report . 

## Projec t Lead/PA I Approval : 

# "'% - 

# I n StudyDirecto r(i fGLP) : Date : Sponso rApprova l (fo rQ>V P protoco l deviations) : NA Date : Technica l Reviewe r(optional) : NA Date : NA Laborator yDepartment,Manage rApproval : Date :

IV . Authorizatio n t o Resum e Wor k Where haltin g o fwor k

occurred , resumptio n o fwor k mus t firs t b e approve d b yLaborator yManagemen t 

## Laborator yDepartment Manage rApproval : N A Date : NA

Deviatio n No . (assigned b

yStud yDirecto ro rTea

m Leade ra

t th e en d ofstudyo rproject ) ETS-4-008. 7 Pag e 1 o f1 Documentatio n o fDeviation s an d Contro l o fNonconformin g Testin g

Pag e 113o f11 3