Effluent Quality Assessment 

Rayonier Performance Fibers, LLC 

Nassau County 

NPDES #FL0000701 

Sampled April 18, 2011 

July 2011

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1 

CONTENTS 

Introduction 

Methods 

Results and Discussion 

Literature Cited 

Tables 

Table 1 Measured algal growth potential (AGP) 

Table 2 Effluent limits, Class III Marine Criteria, chemical data, and 

 qualifiers 

Appendices 

Appendix A Chain of Custody 

Appendix B Bioassay Bench Sheets 

Appendix C SRT Bench Sheets and Statistical Analyses 

Appendix D Organism Receipt 

Appendix E Chemical Analyses of Effluent 

Appendix F Facility Summary, Effluent Limits, and Permit Violations Recorded on 

 Discharge Monitoring Report 

Appendix G EPA End Sheet 
2 

Rayonier Performance Fibers, LLC, Gum Street, Fernandina Beach, Nassau County, Florida, NPDES# 

FL0000701. Effluent composite samples for biological and chemical analyses for this facility were 

collected on April 18, 2011. 

Introduction

Rayonier Performance Fibers, LLC is a chemical cellulose pulp mill. Treated effluent, with the exception 

of discharges associated with clear sewer, is intermittently discharged into the Class III Marine waters of 

the Amelia River during the outgoing tide. (See Appendix F for detailed facility information provided by 

Heather Webber of FDEP Northeast District.) 

Methods 

Samples collected from the EFF-2 and EFF-3 NPDES sampling points were flow-proportionally 

composited and submitted as the EFF-1* sample for this facility’s Outfall D-001 by Eesa Ali and Sharon 

Piltz (FDEP Northeast District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs 

are available on the web at: http://www.floridadep.org/labs/bars/sas/sop/index.htm. 

* EFF-1 represents the combined discharge of treated process water and treated storm water (EFF-3) 

and the rinse water and regenerate water from the zeolite softening units (EFF-2). 

See Appendix A for the completed chain of custody form. 

The 96-hour acute definitive toxicity tests and algal growth potential test were performed following internal 

DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal DEP SOPs are available on 

the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

The facility’s current permit requires that bioassay tests are to be run at the salinity of the receiving water. 

The receiving water salinity was ~ 35.6 ppth which exceeded the test method salinity requirements for 

both test species (A. bahia test range 5 – 30 ppth ± 10%; M. beryllina test range 5 – 32 ppth ± 10%). With 

the permission of the FDEP Northeast District, the test salinity for both bioassay test species was 

determined in accordance with draft toxicity language for Menidia beryllina as found in the facility’s 

proposed NPDES permit renewal. The bioassay test salinity was calculated as ~15.2 ppth which was the 

salinity resulting from the mixture of 30% effluent (salinity 3.9 ppth) with 70% artificial seawater (salinity 

20 ppth). After salinity adjustment and stabilization, the test salinity was ~15.2 ppth. After the test salinity 

was determined, effluent and ASW were adjusted to ~15.2 ppth, and the effluent was diluted to a top 

concentration of 60%. 

Crystal Sea® marine mix artificial sea salts was used for the salinity adjustments. The salt control used 

for each test was prepared by diluting 20 ppth salinity artificial seawater (ASW) with well water to a 

salinity of approximately 3.9 ppth, then salting this solution with artificial sea salts to match the salinity of 

the appropriate test solution. The purpose of the salt controls was to confirm that the addition of artificial 

sea salts did not adversely affect the sample. The Americamysis bahia test had 5% mortality in the salt 

controls and the Menidia beryllina test had 10% mortality in the salt controls. 

The algal growth potential (AGP) sample was salinity-adjusted with artificial sea salts from an apparent 

salinity of approximately 2.37 parts per thousand (ppth) to a salinity of approximately 7.5 ppth. 

Results and Discussion 

 Toxicity Test Results 

Tests were performed April 19 - 23, 2011. 

Americamysis bahia 96-hour acute definitive bioassay – LC50 = 44.3%, 85%* mortality in 60% † 

 sample at 96 hours 
3 

Menidia beryllina 96-hour acute definitive bioassay – LC50 > 60%, 10%* mortality in 60% † 

 sample at 96 hours 

The bioassay samples were acutely toxic to the A. bahia test species; however, the LC50 met the 

required permit limit of 96-hour LC50 > 30% effluent. The bioassay samples did not show toxicity to the 

M. beryllina test organisms. 

†NOTE: The highest concentration of effluent tested was 60%, in accordance with the proposed permit 

for the facility. 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard 

reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that 

correspond to these facility tests. See Appendix D for test organism receipts. 

 Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 3.0 U mg dry wt/L of the saltwater species Dunaliella 

tertiolecta. Raschke and Schultz determined that AGP values above 10.0 mg dry weight/L represent a 

“problem” threshold for marine receiving waters, implying nutrient enrichment (personal communication, 

Marshall Faircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). The ratio (15.5:1) of total 

nitrogen to total phosphorus suggests that the effluent is co-limited by both nitrogen and phosphorus, 

and the ratio (259.4:1) of inorganic nitrogen to orthophosphate suggests that the effluent is limited by 

orthophosphate (Maloney et al. 1978). To determine actual nutrient limitation, a limiting-nutrient algal 

growth potential test with associated nutrient chemistry should be performed (Miller et al. 1978). 

See Table 1 for AGP results and qualifiers. 

 Chemistry Results 

Total residual chlorine was not tested in the bioassay sample in the laboratory due to the tannic 

color of the sample. Total ammonia (3.24 mg N/L) was detected in the bioassay sample in the 

laboratory. The total ammonia concentration in the composite sample collected and preserved 

for chemical analysis was 3.1 mg N/L. Based on the pH, salinity, and temperature of the 

effluent as measured in the laboratory, the calculated unionized ammonia concentration was 

0.03 mg/L. 

Aluminum, arsenic, cadmium, copper, fluoride, iron, lead, nickel, silver, zinc, TSS, and CBOD were 

detected in the effluent at levels that comply with Class III marine water quality criteria (62-302.530, 

F.A.C.) or permit conditions (NPDES# FL0000701). Chromium-III, chloride, sodium and sulfate were 

also detected in the effluent. 2, 4, 6- Trichlorophenol was detected in the effluent between the 

laboratory method detection limits (MDL) and practical quantitation limits (PQL). Chlorophyll was 

undetected in the effluent sample. 

See Table 2 for results of analytes detected in the effluent, corresponding limits, and qualifiers. See 

Appendix E for a complete list of chemical analyses performed. See Appendix B for bioassay lab 

equipment. 

Conclusion 

The flow proportional composited effluent samples prepared from effluent collected from the EFF-2 and 

the EFF-3 NPDES Outfalls for this facility on April 18, 2011, although acutely toxic to the Americamysis 

bahia test organisms during the 96-hour acute definitive bioassays did not exceed unacceptable toxicity 

(LC50 < 30% effluent) in accordance with the facility’s NPDES permit. The bioassay samples were not 

toxic to the Menidia beryllina bioassay test organisms. No growth was detected in the marine AGP test, 

and therefore, the result did not exceed the threshold for marine receiving waters. Effluent water quality 

samples collected on April 18, 2011, did not exceed any applicable water quality standards or violate any 

permit conditions. 
4 

Literature Cited 

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data assessment. 

J. Wat. Poll. Cont. Fed. 59(4): 222-227. 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: Bottle Test. 

National Environmental Research Center Office of Research and Development. U. S. EPA, Corvallis, 

Oregon. 43 p. 

Manahan, S. E. 1994. Environmental Chemistry, 6th edition. Lewis Publishers, USA. 811 pp 

Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz algal assay 

bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p
5 

Inorganic Total

Location N:P ratio N:P ratio

Effluent 3.0 U 259.4 15.5

* -Sample salinity adjusted to ~7 ppt

U - Material analyzed for but not detected; value reported is the method detection limit

(measured)

Table 1. Measured algal growth potential (AGP; mg dry 

weight/L) of the saltwater species Dunaliella tertiolecta 

and ratios of nitrogen to phosphorus for samples 

collected from the Rayonier Performance Fibers, LLC 

plant on April 18, 2011.

AGP

Rayonier Performance Fibers, LLC 

NPDES# FL0000701
6 

Rayonier Performance Fibers, LLC 

NPDES# FL0000701

Class III 

Criteria

Effluent 

Limits

2,4,6-Trichlorophenol ≤ 6.5 a - 1.9 I

Aluminum ≤ 1,500 ≤ 3,000 s 1,740

Arsenic ≤ 50 - 1.05

Cadmium ≤ 8.8 - 0.35

Calcium (mg/L) - - 396

Chromium-III - - 4.4

Copper ≤ 3.7 ≤ 11.5 s 7.5

Iron ≤ 300 ≤ 2,400 s 1,120

Lead ≤ 8.5 ≤ 27.2 s 1.95

Magnesium (mg/L) - - 49.9

Nickel ≤ 8.3 ≤ 66.4 s 8.71

Selenium ≤ 71 - 0.2 U

Silver ≤ 2.3 ≤ 2.3 s 0.14

Sodium (mg/L) - - 772

Zinc ≤ 86 ≤ 410.0 s 126

Ortho-phosphate - - 0.012

Total Phosphorus - - 2

Total Ammonia - Report 3.1

Un-ionized Ammonia - Report 0.03 c

Nitrate and Nitrite - - 0.013

Total Kjeldahl Nitrogen - - 31

Organic Nitrogen - - 27.9 c

Organic Carbon - 270

Total Nitrogen - - 31.013 c

Dissolved Oxygen (mg/L) ≥ 4.0 - 6.9 £

pH (SU) 6.5 - 8.5 6.0 - 8.5 7.2 £

Conductivity (μmhos/cm) - - 7,080 £

Temperature (°C) - Report 25.1 £

Salinity - - 3.9 £

Chlorophyll a (μg/L) - Corrected - - 11 U

Phaeophytin (μg/L) - - 8 U

Total Suspended Solids (mg/L) - ≤ 250.5 p 197

Color (pt-Co) - - 760

CBOD, 5 day (mg/L), N - inhibited - ≤ 215.2 p 84 J

Chloride (mg/L) - - 850

Fluoride (mg/L) ≤ 5.0 - 0.69

Sulfate (mg/L) - - 1600

Flow (MGD) - 26.31 a 17.2 *

Hardness (mg CaCO3) - - 1,194.3 c

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

b - Value is calculated based on hardness

c - Value is calculated

p - 24 hour flow proportioned composite

s - Single sample

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

J - Estimated value

U - Material analyzed for but not detected; value reported is the method detection limit

£ - measurement taken in the FDEP laboratory

* - Measurments taken from 12/09 - 11/10

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and 

chemical data for samples collected from the Rayonier Performance Fibers, LLC 

plant on April 18, 2011.

Effluent

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)
7 

Appendix A. Chain of Custody Form 
8 

Appendix A. (continued) 
9 

Appendix B. Bench Sheets 
10 

Appendix B. (continued) 
11 

Appendix B. (continued) 
12 

Appendix B. (continued) 
13 

Appendix B. (continued) 
14 

Appendix B. (continued) 
15 

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity

Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl2) 

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

09/29/09 22.46 26.17 2.97 20.22 32.11

11/3/09 23.12 26.08 3.04 20.00 32.15

12/1/09 31.91 26.03 2.94 20.16 31.91

1/5/10 25.00 25.91 2.92 20.06 31.75

2/2/10 26.18 25.77 2.84 20.09 31.46

2/9/10 41.45 26.68 4.46 17.76 35.59

3/3/10 28.31 26.78 4.47 17.84 35.72

4/6/10 24.49 26.66 4.50 17.66 35.65

5/4/10 27.02 26.91 4.36 18.20 35.63

6/8/10 30.17 27.15 4.40 18.34 35.95

6/29/10 20.00 26.78 4.68 17.42 36.15

8/3/10 24.49 26.45 4.59 17.27 35.62

8/31/10 30.17 26.68 4.66 17.37 35.99

10/5/10 29.70 26.94 4.67 17.60 36.29

10/26/10 21.53 26.80 4.80 17.20 36.39

11/30/10 27.31 26.76 4.79 17.18 36.35

1/4/11 24.29 26.83 4.74 17.35 36.32

2/15/11 17.08 26.19 5.15 15.88 36.49

3/1/11 23.78 26.07 5.18 15.71 36.43

4/13/11 31.04 26.48 5.24 16.00 36.95

CV = 19.78

Lower Confidence Limit = mean - 2 * Std Dev EPA reference 75th quartile CV = 26%

Upper Confidence Limit = mean + 2 * Std Dev

10

15

20

25

30

35

40

45

50

09/29/09

11/3/0912/1/09

1/5/102/2/102/9/103/3/104/6/105/4/106/8/10

6/29/10

8/3/10

8/31/1010/5/10

10/26/1011/30/10

1/4/11

2/15/11

3/1/11

4/13/11

μg/L CdCl2

Standard Reference Toxicant Data 

Americamysis bahia 96 hour LC50

LC50 Mean LC50 Lower Control Limit Upper Control Limit
16 

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity

Menidia beryllina (inland silverside) - Copper Chloride (CuCl2) as Cu

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

09/29/09 497.5 318.42 88.10 142.21 494.62

10/13/09 426.5 326.12 90.57 144.97 502.27

11/3/09 441.06 329.60 93.69 142.22 516.99

12/1/09 390.28 335.59 93.54 148.51 522.68

1/5/10 387.66 343.92 90.17 163.57 524.26

2/9/10 306.1 350.85 80.73 189.39 512.30

3/3/10 298.9 353.79 77.48 198.82 508.76

3/30/10 333.83 354.48 77.23 200.03 508.94

5/11/10 352.66 359.63 73.21 213.21 506.05

6/8/10 311.45 359.36 73.38 212.59 506.13

6/29/10 315.29 362.43 69.94 222.56 502.31

8/3/10 344.21 367.37 64.53 238.31 496.42

8/31/10 266.23 359.85 67.19 225.46 494.23

10/5/10 340.06 365.40 60.01 245.39 485.42

11/5/10 236.68 360.93 66.11 228.71 493.15

11/30/10 299.75 352.65 62.62 227.41 477.89

1/4/11 274.39 348.59 65.00 218.58 478.60

2/1/11 334.16 345.13 63.77 217.60 472.66

3/1/11 312.31 343.58 64.19 215.20 471.96

3/29/11 227.2 334.81 67.59 199.62 470.00

CV = 20.19

EPA reference 75th quartile CV = 21%

0

100

200

300

400

500

600

09/29/0910/13/09

11/3/0912/1/09

1/5/102/9/103/3/10

3/30/105/11/10

6/8/10

6/29/10

8/3/10

8/31/1010/5/1011/5/10

11/30/10

1/4/112/1/113/1/11

3/29/11

μg/L Cu

Test Date

Standard Reference Toxicant Data 

Menidia beryllina 96-hour LC50

LC50 Mean LC50 Lower Control Limit Upper Control Limit
17 

Appendix C. (continued) 
18 

Appendix C. (continued) 
19 

Appendix C. (continued) 
20 

Appendix C. (continued) 
21 

Appendix C. (continued) 

Americamysis bahia Acute Standard Reference Toxicant Test

DATE: 4‐13‐11            DURATION:   96 h   TOXICANT :  CdCl2

SPECIES:  Americamysis bahia

RAW DATA:  Concentration       Number      Mortalities

   (μg/L)              Exposed

                       .00                  20             0

                   20.00                  20             3

                   30.00                  20             8

                   45.00                  20            19

                   67.00                  20            20

                 100.00                  20            20

  SPEARMAN‐KARBER TRIM:             15.00%

  SPEARMAN‐KARBER ESTIMATES: LC50:   31.04

                 95% LOWER CONFIDENCE:           27.14

                 95% UPPER CONFIDENCE:            35.51

Menidia beryllina Acute Standard Reference Toxicant Test

DATE:   3‐29‐11            DURATION:   96 h    TOXICANT :  CuCl2

SPECIES:  Menidia beryllina

RAW DATA:  Concentration       Number      Mortalities

   (μg/L)             Exposed

                       .00                  20             0

                100.00                  20             0

                200.00                  20             4

                300.00                  20            18

                400.00                  20            20

                500.00                  20            20

SPEARMAN‐KARBER TRIM:               .00%

SPEARMAN‐KARBER ESTIMATES: LC50:  227.20

                95% LOWER CONFIDENCE:         203.80

                95% UPPER CONFIDENCE:           253.30
22 

Appendix D. Test Organism Receipts 
23 

Appendix D. (continued) 
24 

Appendix D. (continued) 
25 

Appendix E. Chemical Analyses performed on the composited effluent from the Rayonier Perfo

LLC, Plant NPDES EFF-2 and EFF-3 sampling points for Outfall D-001, sampled on April 18, 201

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 11:34 EFF-1 AGP/LN Algal Growth Potential 3 U mg DryWt/L 3 4/18/2011 11:34 EFF-1 Chlorophyll/Grain Size/BOD Phaeophytin-a 8 U ug/L 8 4/18/2011 11:34 EFF-1 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 11 U ug/L 14/18/2011 11:34 EFF-1 Chlorophyll/Grain Size/BOD BOD-5 Day,N-Inhib 84 J mg/L 04/18/2011 11:34 EFF-1 Toxicology BSY-AD-SW-Mysid, LC50 44.3 LC50 4/18/2011 11:34 EFF-1 Toxicology BSY-AD-SW-Fish, LC50 60 L LC50 4/18/2011 11:34 EFF-1 Metals Copper 7.5 ug/L 04/18/2011 11:34 EFF-1 Metals Chromium 4.4 ug/L 04/18/2011 11:34 EFF-1 Metals Nickel 8.71 ug/L 04/18/2011 11:34 EFF-1 Metals Aluminum 1740 ug/L 14/18/2011 11:34 EFF-1 Metals Iron 1120 ug/L 34/18/2011 11:34 EFF-1 Metals Calcium 396 mg/L 14/18/2011 11:34 EFF-1 Metals Silver 0.14 ug/L 04/18/2011 11:34 EFF-1 Metals Selenium 0.2 U ug/L 04/18/2011 11:34 EFF-1 Metals Cadmium 0.35 ug/L 04/18/2011 11:34 EFF-1 Metals Arsenic 1.05 ug/L 04/18/2011 11:34 EFF-1 Metals Magnesium 49.9 mg/L 04/18/2011 11:34 EFF-1 Metals Lead 1.95 ug/L 04/18/2011 11:34 EFF-1 Metals Zinc 126 ug/L 5 4/18/2011 11:34 EFF-1 Metals Sodium 772 mg/L 14/18/2011 11:34 EFF-1 Nutrients Kjeldahl Nitrogen 31 mg N/L 14/18/2011 11:34 EFF-1 Nutrients Total-P 2 mg P/L 04/18/2011 11:34 EFF-1 Nutrients O-Phosphate-P 0.012 mg P/L 04/18/2011 11:34 EFF-1 Nutrients TSS 197 mg/L 34/18/2011 11:34 EFF-1 Nutrients Chloride 850 mg Cl/L 4 4/18/2011 11:34 EFF-1 Nutrients Alkalinity 140 mg CaCO3/L 04/18/2011 11:34 EFF-1 Nutrients Color (true) 760 PCU 24/18/2011 11:34 EFF-1 Nutrients Organic Carbon 270 mg C/L 5 4/18/2011 11:34 EFF-1 Nutrients Sulfate 1600 mg SO4/L 14/18/2011 11:34 EFF-1 Nutrients NO2NO3-N 0.013 mg N/L 04/18/2011 11:34 EFF-1 Nutrients Ammonia-N 3.1 mg N/L 0
26 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 11:34 EFF-1 Nutrients Fluoride 0.69 mg F/L 04/18/2011 11:34 EFF-1 Pesticides Butylate 0.21 UJ ug/L 04/18/2011 11:34 EFF-1 Pesticides Fonofos 0.11 UJ ug/L 04/18/2011 11:34 EFF-1 Pesticides Metalaxyl 0.26 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Metribuzin 0.11 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Toxaphene 0.79 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Chlorpyrifos Ethyl 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Ethion 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Parathion Methyl 0.11 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Endrin 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Alachlor 0.63 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Chlorpyrifos Methyl 0.11 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Fenamiphos 0.21 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Naled 0.84 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Alpha-BHC 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Chlordane 0.21 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Heptachlor 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Atrazine 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Hexazinone 0.11 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Norflurazon 0.16 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Prometryn 0.16 U ug/L 04/18/2011 11:34 EFF-1 Pesticides DDT-p,p' 0.032 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Dieldrin 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Methoxychlor 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Ametryn 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Azinphos Methyl 0.21 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Diazinon 0.053 UJ ug/L 04/18/2011 11:34 EFF-1 Pesticides Beta-BHC 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Endrin Aldehyde 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Mevinphos 0.21 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Simazine 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Endosulfan I 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Bromacil 0.21 U ug/L 0
27 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 11:34 EFF-1 Pesticides Malathion 0.16 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Metolachlor 0.53 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Parathion Ethyl 0.16 UJ ug/L 04/18/2011 11:34 EFF-1 Pesticides Phorate 0.053 U ug/L 04/18/2011 11:34 EFF-1 Pesticides DDE-p,p' 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Endosulfan II 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Gamma-BHC 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Heptachlor Epoxide 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Ethoprop 0.11 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Aldrin 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides DDD-p,p' 0.021 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Delta-BHC 0.011 U ug/L 04/18/2011 11:34 EFF-1 Pesticides Endosulfan Sulfate 0.021 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants 1,2,4-Trichlorobenzene 1 U ug/L 1 

4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,4,6-Trichlorophenol 1.9 I ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Phenol 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Phenanthrene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Pentachlorophenol 3.1 U ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants Naphthalene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Isophorone 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Hexachlorobutadiene 3.1 U ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants 4-Bromophenyl phenyl ether 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 16 U ug/L 14/18/2011 11:34 EFF-1 Priority Organic Pollutants Di-n-octyl phthalate 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,4-Dinitrophenol 16 U ug/L 14/18/2011 11:34 EFF-1 Priority Organic Pollutants 3,3'-Dichlorobenzidine 42 UJ ug/L 44/18/2011 11:34 EFF-1 Priority Organic Pollutants Acenaphthylene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzidine 100 UJ ug/L 14/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzo(a)anthracene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzo(b)fluoranthene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzo(k)fluoranthene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Bis(2-chloroethyl)ether 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Dimethyl phthalate 10 U ug/L 1
28 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2-Nitrophenol 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 4-Chlorophenyl phenyl ether 2.1 U ug/L 2

4/18/2011 11:34 EFF-1 Priority Organic Pollutants Butyl benzyl phthalate 5.2 U ug/L 54/18/2011 11:34 EFF-1 Priority Organic Pollutants Fluorene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Hexachloroethane 3.1 UJ ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants N-Nitrosodimethylamine 2.1 U ug/L 24/18/2011 11:34 EFF-1 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 3.1 U ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,4-Dinitrotoluene 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2-Chloronaphthalene 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 3.1 UJ ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants Acenaphthene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Chrysene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Di-n-butyl phthalate 5.2 U ug/L 54/18/2011 11:34 EFF-1 Priority Organic Pollutants Dibenzo(a,h)anthracene 0.21 UJ ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Diethyl phthalate 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Fluoranthene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,6-Dinitrotoluene 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2-Chlorophenol 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Anthracene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.21 UJ ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Pyrene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants 4-Nitrophenol 16 U ug/L 14/18/2011 11:34 EFF-1 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 3.1 U ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants Hexachlorocyclopentadiene 3.1 UJ ug/L 34/18/2011 11:34 EFF-1 Priority Organic Pollutants N-Nitrosodi-n-propylamine 2.1 U ug/L 24/18/2011 11:34 EFF-1 Priority Organic Pollutants Nitrobenzene 2.1 U ug/L 24/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,4-Dichlorophenol 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants 2,4-Dimethylphenol 10 U ug/L 14/18/2011 11:34 EFF-1 Priority Organic Pollutants 4-Chloro-3-methylphenol 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzo(a)pyrene 0.21 U ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Bis(2-chloroethoxy)methane 1 U ug/L 1 4/18/2011 11:34 EFF-1 Priority Organic Pollutants Benzo(g,h,i)perylene 0.21 UJ ug/L 04/18/2011 11:34 EFF-1 Priority Organic Pollutants Hexachlorobenzene 1 U ug/L 1 
29 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 12:08 BLANK Metals Arsenic 0.25 U ug/L 04/18/2011 12:08 BLANK Metals Copper 0.5 U ug/L 04/18/2011 12:08 BLANK Metals Nickel 0.25 U ug/L 04/18/2011 12:08 BLANK Metals Zinc 5 U ug/L 5 4/18/2011 12:08 BLANK Metals Cadmium 0.05 U ug/L 04/18/2011 12:08 BLANK Metals Chromium 0.3 U ug/L 04/18/2011 12:08 BLANK Metals Silver 0.025 U ug/L 04/18/2011 12:08 BLANK Metals Lead 0.2 U ug/L 04/18/2011 12:08 BLANK Metals Sodium 0.5 U mg/L 04/18/2011 12:08 BLANK Metals Aluminum 5 U ug/L 5 4/18/2011 12:08 BLANK Metals Iron 30 U ug/L 34/18/2011 12:08 BLANK Metals Magnesium 0.04 U mg/L 04/18/2011 12:08 BLANK Metals Selenium 0.2 U ug/L 04/18/2011 12:08 BLANK Metals Calcium 0.075 U mg/L 04/18/2011 12:08 BLANK Nutrients Total-P 0.004 U mg P/L 04/18/2011 12:08 BLANK Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 04/18/2011 12:08 BLANK Nutrients Organic Carbon 1 U mg C/L 1 4/18/2011 12:08 BLANK Nutrients Chloride 0.2 U mg Cl/L 04/18/2011 12:08 BLANK Nutrients NO2NO3-N 0.004 U mg N/L 04/18/2011 12:08 BLANK Nutrients Sulfate 0.2 U mg SO4/L 04/18/2011 12:08 BLANK Nutrients Color (true) 2 U PCU 2 4/18/2011 12:08 BLANK Nutrients Fluoride 0.035 U mg F/L 04/18/2011 12:08 BLANK Nutrients O-Phosphate-P 0.004 U mg P/L 04/18/2011 12:08 BLANK Nutrients Alkalinity 0.65 U mg CaCO3/L 04/18/2011 12:08 BLANK Nutrients Ammonia-N 0.01 U mg N/L 04/18/2011 12:08 BLANK Pesticides Ethoprop 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Gamma-BHC 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Hexazinone 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Naled 0.8 U ug/L 04/18/2011 12:08 BLANK Pesticides Delta-BHC 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Endrin 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Methoxychlor 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Toxaphene 0.75 U ug/L 0
30 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 12:08 BLANK Pesticides Alachlor 0.6 U ug/L 04/18/2011 12:08 BLANK Pesticides Norflurazon 0.15 U ug/L 04/18/2011 12:08 BLANK Pesticides Heptachlor Epoxide 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Ethion 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Metolachlor 0.5 U ug/L 04/18/2011 12:08 BLANK Pesticides Parathion Methyl 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Phorate 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Simazine 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Endosulfan I 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Endosulfan II 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Ametryn 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Alpha-BHC 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Beta-BHC 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Chlordane 0.2 U ug/L 04/18/2011 12:08 BLANK Pesticides DDE-p,p' 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides DDT-p,p' 0.03 U ug/L 04/18/2011 12:08 BLANK Pesticides Dieldrin 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Chlorpyrifos Ethyl 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Chlorpyrifos Methyl 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Aldrin 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Endrin Aldehyde 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Bromacil 0.2 U ug/L 04/18/2011 12:08 BLANK Pesticides Parathion Ethyl 0.15 U ug/L 04/18/2011 12:08 BLANK Pesticides Endosulfan Sulfate 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Diazinon 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Malathion 0.15 U ug/L 04/18/2011 12:08 BLANK Pesticides Metalaxyl 0.25 U ug/L 04/18/2011 12:08 BLANK Pesticides Mevinphos 0.2 U ug/L 04/18/2011 12:08 BLANK Pesticides Prometryn 0.15 U ug/L 04/18/2011 12:08 BLANK Pesticides DDD-p,p' 0.02 U ug/L 04/18/2011 12:08 BLANK Pesticides Heptachlor 0.01 U ug/L 04/18/2011 12:08 BLANK Pesticides Atrazine 0.05 U ug/L 04/18/2011 12:08 BLANK Pesticides Azinphos Methyl 0.2 U ug/L 0
31 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 12:08 BLANK Pesticides Butylate 0.2 U ug/L 04/18/2011 12:08 BLANK Pesticides Fonofos 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Metribuzin 0.1 U ug/L 04/18/2011 12:08 BLANK Pesticides Fenamiphos 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants 2,4,6-Trichlorophenol 1 U ug/L 1 

4/18/2011 12:08 BLANK Priority Organic Pollutants 2,4-Dichlorophenol 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 4-Chloro-3-methylphenol 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Butyl benzyl phthalate 5.1 U ug/L 54/18/2011 12:08 BLANK Priority Organic Pollutants Hexachlorocyclopentadiene 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants N-Nitrosodi-n-propylamine 2 U ug/L 2 4/18/2011 12:08 BLANK Priority Organic Pollutants Phenol 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 3,3'-Dichlorobenzidine 40 UJ ug/L 44/18/2011 12:08 BLANK Priority Organic Pollutants Acenaphthene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Anthracene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Benzidine 100 UJ ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants Chrysene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Di-n-butyl phthalate 5.1 U ug/L 54/18/2011 12:08 BLANK Priority Organic Pollutants Isophorone 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants N-Nitrosodimethylamine 2 U ug/L 2 4/18/2011 12:08 BLANK Priority Organic Pollutants Pyrene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Benzo(g,h,i)perylene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Bis(2-chloroethyl)ether 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Naphthalene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants 2-Chlorophenol 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants Benzo(a)anthracene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Diethyl phthalate 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Nitrobenzene 2 U ug/L 2 4/18/2011 12:08 BLANK Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants 2,4-Dinitrotoluene 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 2-Nitrophenol 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 4-Bromophenyl phenyl ether 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Benzo(k)fluoranthene 0.2 U ug/L 0
32 

Appendix E. (continued) 

Date Sampled Field ID Analysis Group Component Result Remark Units M

4/18/2011 12:08 BLANK Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 15 U ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants Di-n-octyl phthalate 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Fluoranthene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Hexachlorobenzene 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants 2,6-Dinitrotoluene 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Acenaphthylene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Benzo(b)fluoranthene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Dimethyl phthalate 10 U ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants Fluorene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Hexachlorobutadiene 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants 1,2,4-Trichlorobenzene 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants Bis(2-chloroisopropyl)ether 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants Dibenzo(a,h)anthracene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Hexachloroethane 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Pentachlorophenol 3 U ug/L 3 4/18/2011 12:08 BLANK Priority Organic Pollutants 2,4-Dimethylphenol 10 U ug/L 14/18/2011 12:08 BLANK Priority Organic Pollutants 2-Chloronaphthalene 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants 4-Chlorophenyl phenyl ether 2 U ug/L 2 4/18/2011 12:08 BLANK Priority Organic Pollutants Benzo(a)pyrene 0.2 U ug/L 04/18/2011 12:08 BLANK Priority Organic Pollutants Bis(2-chloroethoxy)methane 1 U ug/L 1 4/18/2011 12:08 BLANK Priority Organic Pollutants Phenanthrene 0.2 U ug/L 0
33 

Appendix F. Facility Summary, Effluent Limits, and Permit Violations 

Recorded on Discharge Monitoring Report 
34 

Appendix F. (continued) 
35 

Appendix F. (continued) 
36 

Appendix F. (continued) 
37 

Appendix F. (continued) 
38 

Appendix F. (continued) 
39 

Appendix F. (continued) 
40 

Appendix G. EPA End Sheet 

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 7 0 1 11 12 1 1 0 4 1 8 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 7 0 1 11 12 1 1 0 4 1 8 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 7 0 1 11 12 1 1 0 4 1 8 18 S 19 S 20 2

66

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Rayonier Performance Fibers, LLC, plant effluent 

sampled on April 18, 2011. NPDES# FL0000701.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Rayonier Performance Fibers, 

LLC, plant effluent sampled on April 18, 2011. NPDES# 

FL0000701.

Biological Analyses of Rayonier Performance Fibers, LLC, plant 

effluent sampled on April 18, 2011. NPDES# FL0000701.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)