# F -1 October 2011

Document No. 12699-001-500 

# A ppendix F

Number of organisms and survival rates at different stages during

the brown trout reproduction studyTreatmentInitial eggs# deadalevins(hatch - su) % hatch% eggmortality# preserved@ suwet wt15d PSUtarget% survival@ su# preservedat end# forwts/lengthsDead in15d PSUMissing TE% survival(hatchterm)% survival@ term% survival(total)SC-0016006 23.8% 76.2%38 1 100 22.8%77 20 198.9% 97.0% 22.7%SC-0026002 22.8% 77.2%34 1 100 22.5%79 20 199.5% 99.0% 22.3%SC-0036006 56.7% 43.3%233 1 100 55.7%69 20 1 9 1 98.0% 98.9% 54.7%SC-004600 23 31.2% 68.9%60 1 100 27.3%80 20 396.2% 100.0% 27.3%SC-0056006 11.7% 88.3%0 1 62 10.7%42 20 01 99.0% 100.0% 10.7%SC-0066002 93.2% 6.8%457 1 100 92.8%78 20 199.5% 98.0% 92.7%SC-007600 22 30.5% 69.5%61 1 100 26.8%76 20 395.8% 96.0% 26.3%SC-0086006 66.0% 34.0%289 1 100 65.0%70 20 0 1099.0% 100.0% 65.0%SPC-0016008 99.3% 0.7%488 - 100 98.0%80 20 098.7% 100.0% 98.0%SPC-002200 100.0% 0.0%0 - 20 100.0%0 20 0100.0% 100.0% 100.0%SPC-003600 20 97.5% 2.5%465 - 100 94.2%80 20 096.7% 100.0% 94.2%SPC-004210 100.0% 0.0%0 - 21 100.0%0 20 0100.0% 100.0% 100.0%SPC-0056008 98.8% 1.2%485 - 100 97.5%76 20 098.0% 96.0% 96.8%SPC-006600 22 99.7% 0.3%476 - 100 96.0%80 20 096.3% 100.0% 96.0%LSV2C-002 600 14 99.0% 1.0%480 - 100 96.7%64 20 0 1697.6% 100.0% 96.6%LSV2C-003 400 342 93.5% 6.5%0 - 32 8.0%09 238.8% 28.1% 2.25%LSV2C-004 500 102 50.6% 49.4%0 - 151 30.2%64 20 6766.2% 55.6% 16.8%LSV2C-005 300 103 71.3% 28.7%0 - 111 37.0%49 20 4251.7% 62.2% 23.0%LSV2C-006--- 100.0%- - - --- --LSV2C-007--- 100.0%- - - --- --LSV2C-008 3005 88.3% 11.7%160 - 100 86.7%34 20 1 4695.9% 98.2% 84.25%LSV2C-010 100 62 87.0% 13.0%0 - 25 25.0%0 11 1424.0% 44.0% 11.0%LSV2C-012 600 16 98.3% 1.7%474 - 100 95.7%80 20 097.4% 100.0% 95.7%LSV2C-016 600 20 95.0% 5.0%449 - 101 91.7%81 20 096.7% 100.0% 91.7%LSV2C-017 300 22 71.3% 28.7%92 - 100 64.0%58 20 3 1989.2% 96.3% 60.5%LSV2C-019 500 22 94.2% 5.8%349 - 100 89.8%41 20 0 3994.7% 100.0% 88.9%LSV2C-020 4005 89.3% 10.8%252 - 100 88.0%44 20 0 3697.6% 100.0% 86.8%LSV2C-021 600 286 69.3% 30.7%0 - 130 21.7%69 20 4145.5% 68.5% 14.8%CC-150-009 6008 28.5% 71.5%62 - 100 27.2%80 20 198.3% 99.0% 27.0%CC-150-011 3002 96.0% 4.0%185 - 100 95.3%81 20 098.7% 100.0% 95.3%CC-150-012 3507 88.9% 11.2%204 - 100 86.86%77 20 198.1% 97.0% 86.0%CC-150-013 600 42 66.7% 33.3%258 - 100 59.7%52 20 2 2693.0% 97.3% 57.5%CC-150-015 6003 78.3% 21.7%367 - 100 77.8%78 20 098.9% 98.0% 77.5%CC-150-016 6003 14.8% 85.2%0 - 86 14.3%23 20 0 4399.7% 100.0% 15.4%CC-150-017 2507 89.2% 10.8%116 - 100 86.4%47 20 0 3397.2% 100.0% 84.3%CC-150-018 600 17 87.2% 12.8%405 - 100 84.3%81 20 096.5% 100.0% 84.3%CC-150-020 6006 97.3% 2.7%478 - 100 96.3%80 20 098.9% 100.0% 96.3%CC-350-006 600 22 71.7% 28.3%307 - 101 68.0%79 20 296.3% 98.0% 67.7%CC-350-007 6006 29.7% 70.3%72 - 100 28.7%59 20 1 2098.6% 98.8% 26.0%CC-350-008 600 19 67.7% 32.3%287 - 100 64.5%51 20 1 2897.1% 98.6% 62.6%su=swimuppsu=post swimupTE=tech errorwt= weight#s at swimup#s at test termination#s at hatchNumber of Organisms and Survival Rates at Different Stages During Brown Trout Reproductive Study

# G -1 October 2011

Document No. 12699-001-500 

# A ppendix G

Length and dry weight measurements for juvenile brown trout

from reproductive studyPage __ of __Length and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsSaratooga Hatchery (SC)SC001std length(mm)SC001dry wt(mg)SC002std length(mm)SC002dry wt(mg)SC003std length(mm)SC003dry wt(mg)SC004std length(mm)SC004dry wt(mg)SC005std length(mm)SC005dry wt(mg)SC006std length(mm)SC006dry wt(mg)SC007std length(mm)SC007dry wt(mg)A22 19.540 22 21.350 23 31.120 24 31.120 21 22.060 23 16.720 21 17.800B22 15.090 22 15.790 24 25.330 25 30.830 22 27.190 21 16.380 23 22.410C24 20.700 22 19.980 23 24.970 23 25.840 22 19.060 22 13.470 22 21.110D21 16.590 24 25.460 22 22.620 23 25.400 20 21.700 22 17.490 21 20.520E21 13.980 22 17.220 23 21.020 24 21.520 21 21.240 21 22.010 19 13.590F22 20.420 23 16.040 24 29.480 21 20.230 23 16.820 21 22.080 23 24.160G22 22.900 22 17.020 23 22.110 24 29.710 24 14.990 21 21.930 19 16.610H22 21.730 22 20.350 24 27.300 24 32.100 24 20.370 22 22.130 21 15.700I20 13.810 23 23.060 22 23.290 25 37.230 21 17.150 22 17.540 21 22.550J22 25.660 24 19.680 24 27.870 21 19.840 22 22.680 22 14.090 19 14.220K23 24.510 23 21.280 20 15.390 21 21.150 20 16.660 21 10.350 23 25.370L22 19.070 21 15.320 22 16.570 22 22.070 22 14.030 24 19.280 21 19.080M23 20.790 23 23.250 23 26.610 22 23.410 22 16.350 21 16.520 22 17.730N22 18.700 21 14.220 23 21.800 22 24.140 20 18.100 22 22.120 22 18.810O21 20.200 22 15.120 21 23.220 22 24.400 23 17.730 23 20.760 20 17.210P22 16.030 21 20.140 26 22.730 19 8.840 21 13.540 21 19.360 21 19.450Q20 10.060 21 18.220 22 14.700 23 28.330 22 17.060 22 16.390 19 13.530R22 21.590 21 15.380 22 13.560 21 17.960 22 24.120 22 18.610 20 13.620S18 10.990 22 15.420 24 20.720 20 10.860 21 18.700 21 20.890 24 24.740T18 14.400 22 15.100 22 21.630 18 9.950 23 28.160 22 16.680 20 13.130Avg21.45 18.3380 22.15 18.4700 22.85 22.6020 22.20 23.2465 21.80 19.3855 21.80 18.2400 21.05 18.5670Std1.504 4.308 0.933 3.287 1.309 4.813 1.908 7.503 1.196 4.032 0.834 3.297 1.504 3.947n20202020202020Page __ of __ABCDEFGHIJKLMNOPQRSTAvgStdnLength and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsSaratooga Hatchery (SC)SC008std length(mm)SC008dry wt(mg)SC008 (2)std length(mm)SC008 (2)std lengthbeforeisopropylSC008 (2)dry wt(mg)SC008 (2)wet wt(mg)21 13.780212317.360 131.00019 10.360222319.090 142.10023 18.880222213.920 104.30021 16.370212316.010 114.70022 15.450232420.430 153.30023 22.020222315.120 118.80022 20.260212315.290 113.30023 21.530192012.640 94.70021 14.800202215.540 111.70020 13.200212319.190 131.30021 15.32021 12.31020 12.75021 12.00022 15.71020 12.23020 11.20022 16.32021 14.63020 13.25021.15 15.1185 21.20 22.60 16.4590 121.521.137 3.338 1.135 1.075 2.499 17.80420 10 10Page __ of __Length and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsSpring Creek Hatchery (SPC)SPC 001std length(mm)SPC 001dry wt(mg)SPC 002std length(mm)SPC 002dry wt(mg)SPC 003std length(mm)SPC 003dry wt(mg)SPC 004std length(mm)SPC 004dry wt(mg)SPC 005std length(mm)SPC 005dry wt(mg)SPC 006std length(mm)SPC 006dry wt(mg)A27 27.100 22 25.070 22 27.310 25 28.360 23 22.770 23 19.220B26 12.040 26 21.560 24 18.710 24 21.040 27 23.330 23 25.440C25 17.210 23 28.420 23 15.360 22 22.380 23 16.450 26 33.260D26 19.410 25 17.460 23 18.670 23 23.370 24 23.030 27 20.660E23 14.130 23 18.030 24 21.350 24 19.380 23 20.790 23 22.510F21 25.640 22 24.890 26 21.040 25 30.400 21 15.890 23 15.350G25 17.650 21 17.050 27 21.340 25 20.040 24 18.820 20 19.470H25 31.060 25 17.410 28 27.550 22 27.050 23 18.330 22 22.200I27 25.630 23 15.500 21 31.630 21 19.100 24 35.790 25 22.340J24 13.110 23 22.740 22 20.300 25 21.360 25 15.820 23 15.840K25 23.650 25 27.260 25 10.890 22 33.380 25 23.030 23 22.550L28 27.860 21 18.590 23 22.570 25 25.420 21 13.610 25 23.770M23 29.700 24 24.110 21 29.820 22 16.320 21 14.270 23 34.550N21 17.460 22 18.270 21 19.840 27 15.420 20 17.200 21 17.510O24 22.300 23 22.790 23 16.650 20 15.810 20 21.370 23 36.450P23 21.660 22 17.710 21 18.950 23 16.360 26 22.150 22 20.340Q25 31.230 22 14.910 22 20.000 27 16.670 22 19.210 22 20.750R21 31.270 23 18.280 21 30.640 24 25.100 22 15.840 24 19.890S22 23.150 20 20.400 25 17.920 22 14.720 24 34.000 22 22.650T26 20.020 22 26.580 24 25.600 21 19.590 22 24.150 23 12.960Avg24.35 22.5640 22.85 20.8515 23.30 21.8070 23.45 21.5635 23.00 20.7925 23.15 22.3855Std2.084 6.115 1.531 4.116 2.080 5.416 1.959 5.326 1.919 5.826 1.631 6.134n202020202020Page __ of __Length and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsCC-350CC-350006 stdlength(mm)CC-350006 drywt (mg)CC-350007 stdlength(mm)CC-350007 drywy (mg)CC-350008 stdlength(mm)CC-350008 drywt (mg)A23 13.250 20 12.130 22 9.470B20 11.680 21 8.570 21 10.040C22 10.240 23 8.040 21 8.890D20 14.390 21 7.700 22 17.650E22 14.430 23 15.750 21 11.710F22 9.330 21 12.850 19 17.750G22 16.810 24 17.980 22 10.830H20 8.820 21 6.890 20 9.000I23 17.060 23 10.410 20 14.820J20 16.820 22 9.170 21 8.870K21 8.760 23 13.370 19 10.820L20 9.440 20 16.580 20 10.050M20 14.070 21 18.080 22 9.470N22 10.310 19 11.150 20 11.760O21 17.500 20 10.740 20 13.120P23 10.380 21 7.660 20 9.370Q22 8.000 20 10.480 20 10.300R21 14.170 20 8.010 20 14.400S23 9.300 21 7.260 21 16.860T21 12.090 22 7.360 20 16.600Avg21.40 12.3425 21.30 11.0090 20.55 12.0890Std1.142 3.148 1.342 3.676 0.945 3.130n202020Page __ of __Length and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsCC-150CC-150009 stdlength(mm)CC-150009 drywt (mg)CC-150011 stdlength(mm)CC-150011 drywt (mg)CC-150012 stdlength(mm)CC-150012 drywt (mg)CC-150013 stdlength(mm)CC-150013 drywt (mg)CC-150015 stdlength(mm)CC-150015 drywt (mg)CC-150016 stdlength(mm)CC-150016 drywt (mg)CC-150017 stdlength(mm)CC-150017 drywt (mg)A22 12.190 20 11.660 22 15.840 23 17.150 23 15.800 21 21.400 21 11.300B20 15.970 20 9.890 21 8.630 22 11.860 20 14.000 23 15.430 21 9.480C24 8.200 20 6.200 22 14.970 20 17.660 25 11.390 25 17.090 21 10.570D20 13.430 20 8.040 20 9.110 22 11.810 24 17.260 24 15.620 20 9.870E21 17.200 19 5.850 22 10.960 22 20.140 21 15.060 22 22.300 21 11.310F22 8.810 19 11.940 20 13.340 21 11.360 23 8.410 23 10.710 21 8.020G20 14.270 20 6.690 21 13.100 23 23.340 22 15.970 22 16.070 21 15.410H24 13.240 20 12.220 22 15.510 22 13.250 23 14.740 23 25.300 21 7.060I20 15.060 21 11.370 21 9.520 22 16.440 23 12.270 23 16.180 22 7.090J22 16.900 21 9.430 20 13.140 23 17.370 23 17.180 24 10.320 22 7.990K23 17.780 20 11.140 21 14.410 25 13.480 22 19.120 24 11.660 20 10.710L23 12.570 22 11.830 22 12.490 22 20.130 24 14.210 21 17.990 23 13.780M22 17.280 19 6.660 22 19.730 25 12.870 22 16.180 23 12.680 21 9.940N20 8.250 20 7.990 21 10.560 24 15.890 23 18.170 22 15.270 21 9.550O23 11.520 21 7.690 23 13.840 22 10.210 21 18.080 22 21.730 20 10.460P22 9.430 21 9.590 22 9.380 23 14.270 21 12.320 22 19.650 21 13.030Q23 17.190 19 9.150 21 15.410 23 16.330 22 13.010 25 13.090 20 11.940R22 11.480 21 7.540 21 14.360 24 17.700 23 15.700 23 18.080 20 8.310S22 13.680 19 13.410 22 10.300 21 13.790 24 6.850 22 16.990 20 14.260T22 9.300 20 6.680 23 12.200 22 16.600 22 14.000 22 17.160 22 12.970Avg21.85 13.1875 20.10 9.2485 21.45 12.8400 22.55 15.5825 22.55 14.4860 22.80 16.7360 20.95 10.6525Std1.309 3.254 0.852 2.335 0.887 2.822 1.276 3.367 1.234 3.152 1.152 3.987 0.826 2.382n20202020202020Page __ of __CC-150ABCDEFGHIJKLMNOPQRSTAvgStdnLength and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsCC-150018 stdlength(mm)CC-150018 drywt (mg)CC-150020 stdlength(mm)CC-150020 drywt (mg)21 16.610 20 12.04020 14.100 21 5.70022 16.740 19 15.55020 10.730 22 6.79019 5.700 20 14.28022 14.280 21 7.59023 15.640 22 7.05022 13.500 22 18.36020 16.560 20 11.32020 11.270 21 10.96021 10.500 20 7.69022 12.970 22 13.10022 8.000 20 14.18022 13.310 18 10.60022 18.330 21 8.36021 8.330 18 13.11021 14.540 19 14.81022 15.130 19 7.29022 12.720 21 14.98022 15.930 18 11.19021.30 13.2445 20.20 11.24751.031 3.302 1.361 3.55320 20Page __ of __Length and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsLSV2CLSV2C002 stdlength(mm)LSV2C002 drywt (mg)LSV2C003 stdlength(mm)LSV2C003 drywt (mg)LSV2C004 stdlength(mm)LSV2C004 drywt (mg)LSV2C005 stdlength(mm)LSV2C005 drywt (mg)LSV2C008 stdlength(mm)LSV2C008 drywt (mg)LSV2C010 stdlength(mm)LSV2C010 drywt (mg)LSV2C012 stdlength(mm)LSV2C012 drywt (mg)A18 11.850 22 11.760 22 9.620 19 7.080 21 8.870 20 8.010 22 13.810B20 17.480 20 7.080 21 11.310 21 12.370 20 9.980 20 6.230 21 12.540C20 12.730 21 7.310 21 8.910 19 7.400 20 10.300 20 10.640 22 12.370D21 8.140 22 8.010 22 7.600 23 11.710 21 8.950 21 6.770 22 15.000E19 9.720 21 12.110 20 11.960 20 10.580 21 8.370 20 7.600 22 12.700F20 8.020 21 7.890 22 6.700 20 7.370 21 11.420 21 7.290 22 13.280G20 11.680 21 9.420 21 9.520 20 16.890 21 9.490 19 9.570 23 12.550H22 10.130 22 9.850 21 10.130 20 8.140 20 12.150 21 14.100 22 13.390I21 13.820 22 13.960 23 13.810 21 15.330 21 9.710 19 7.370 22 12.400J19 6.82020 10.720 19 10.990 21 8.670 19 4.690 22 13.470K20 10.44021 9.640 20 13.220 20 8.420 19 6.740 22 12.810L20 6.51020 9.310 20 9.950 20 8.51021 13.450M19 9.84020 10.450 19 10.270 21 8.76022 13.460N21 12.09023 10.990 21 11.540 20 8.67022 14.100O20 13.28021 10.670 19 12.300 20 7.92022 13.530P18 10.03021 13.330 21 13.230 20 10.23022 14.940Q22 10.78021 8.040 20 11.210 21 9.02022 12.850R21 15.61021 7.100 22 6.920 20 10.74023 12.120S22 6.29021 6.890 21 12.750 20 7.94022 12.480T19 16.13022 12.880 20 10.090 20 8.72022 13.870Avg20.10 11.0695 21.33 9.7100 21.20 9.9790 20.25 10.9670 20.45 9.3420 19.91 8.0918 22.00 13.2560Std1.210 3.161 0.707 2.424 0.894 2.082 1.070 2.712 0.510 1.147 0.831 2.546 0.459 0.814n2092020201120Page __ of __LSV2CABCDEFGHIJKLMNOPQRSTAvgStdnLength and dry weight data for juvenile brown trout from each treatment12699-001filename: length & wt.xlsLSV2C016 stdlength(mm)LSV2C016 drywt (mg)LSV2C017 stdlength(mm)LSV2C017 drywt (mg)LSV2C019 stdlength(mm)LSV2C019 drywt (mg)LSV2C020 stdlength(mm)LSV2C020 drywt (mg)LSV2C021 stdlength(mm)LSV2C021 drywt (mg)22 17.320 24 25.130 23 26.560 21 13.920 22 8.32019 15.730 23 20.260 25 23.350 21 12.980 23 8.50022 16.060 23 15.130 23 21.680 21 10.990 19 10.38025 14.700 23 24.680 24 18.840 23 7.890 20 10.28022 9.710 23 17.080 23 25.970 21 10.270 20 9.96023 17.160 23 21.450 21 15.450 23 8.570 20 16.84023 16.230 25 13.590 25 13.100 22 8.090 19 7.71022 12.390 23 19.750 23 10.410 22 8.660 20 10.69020 24.040 23 17.630 23 17.300 21 13.900 22 15.69020 11.710 24 21.390 26 19.830 22 9.790 21 4.55022 27.380 24 24.930 23 13.690 21 15.120 18 11.96022 14.060 24 18.110 22 23.830 21 9.360 19 12.53021 9.310 25 15.480 22 10.580 21 16.620 20 10.47021 21.020 24 17.330 26 33.340 24 14.030 21 5.95024 18.480 23 19.570 24 18.080 21 9.520 18 5.51021 18.270 25 16.470 24 23.380 21 14.690 23 13.28023 12.900 24 9.940 24 17.950 22 12.750 19 9.94023 7.430 21 17.680 26 21.150 22 14.230 21 18.35020 20.420 25 23.820 22 14.510 21 9.250 19 16.00021 12.390 25 18.130 24 17.390 24 10.840 20 6.21021.80 15.8355 23.70 18.8775 23.65 19.3195 21.75 11.5735 20.20 10.65601.473 4.974 1.031 3.966 1.424 5.764 1.020 2.701 1.473 3.9012020202020

# Page __ of __

Average length and weight of brown trout ( Salmo trutta ) at test termination.

12699-001-300

Note: each value represents the average of ~20 individual fish

f ilename: length & wt.xls



# avg std length

(mm) 

# avg dry

wt (mg) avg std



# length

b efore

Isopropyl 

# avg wet

wt (mg) 

# Grand

A vg std

length 

# Grand

a vg dry

wt (mg)
Saratoga

Hatchery



# SC-001 2 1.45 18.338 (measured after storage in isopropyl)



# SC-002 22.15 18.470 (measured after storage in isopropyl)



# SC-003 22.85 22.602 (measured after storage in isopropyl)



# SC-004 22.20 23.247 (measured after storage in isopropyl)



# SC-005 21.80 19.386 (measured after storage in isopropyl)



# SC-006 21.80 18.240 (measured after storage in isopropyl)



# SC-007 21.05 18.567 (measured after storage in isopropyl)



# SC-008 21.15 15.119 21.81 19.2459 (measured after storage in isopropyl) 

# SC-008 (2) 21.20 16.459 22.60 121.52 (measured prior to storage in isopropyl)

(all other fish measured prior to storage in isopropyl)



# Spring Creek

Hatchery



# SPC-001 2 4.35 22.564

SPC-002 22.85 20.852

SPC-003 23.30 21.807

SPC-004 23.45 21.564

SPC-005 23.00 20.793

SPC-006 23.15 22.385 23.35 21.6607

High



# LSV2C-002 2 0.10 11.069

LSV2C-003 21.33 9.710

LSV2C-004 21.20 9.979

LSV2C-005 20.25 10.967

LSV2C-006

LSV2C-007

LSV2C-008 20.45 9.342

LSV2C-010 19.91 8.092

LSV2C-012 22.00 13.256

LSV2C-016 21.80 15.835

LSV2C-017 23.70 18.878

LSV2C-019 23.65 19.320

LSV2C-020 21.75 11.574

LSV2C-021 20.20 10.656 21.36 12.3898

Bkd



# CC-150-009 2 1.85 13.188

CC-150-011 20.10 9.249

CC-150-012 21.45 12.840

CC-150-013 22.55 15.583

CC-150-015 22.55 14.486

CC-150-016 22.80 16.736

CC-150-017 20.95 10.652

CC-150-018 21.30 13.245

CC-150-020 20.20 11.248 21.53 13.0250

Low



# CC-350-006 2 1.40 12.343

CC-350-007 21.30 11.009

CC-350-008 20.55 12.089 21.08 11.8135

# APPENDIX B
Detail Figures for Locations where Brown Trout were Collected

# CC75 CC150REDD CC350



# C C 

# r r



# o o

w w C C r r



# e e



# e e

k k D

e e



# e e



# r r C 

# r r

e e

e e

k k

W 

# a a

r r

m 

# C r r e e

e e

k k S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_CROWCKUP_OCT07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 1 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

OCTOBER 2007 FISHING REACHES:

CROW CREEK UPSTREAM

0 1,000 2,000 500

Feet 

# Map Extent Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Surface Water Sampling Location

Fishing Reach

# LSV2C

CC1A CC3A

C C r

r o o

w w 

# C C r r

e e 

# e

e k

k

S S

a a

g g

e e

C C

r r 

# e e



# e e

k k



# R R

o o



# c c



# k k

C

r r e e e e k k 

# S S

o o

u u t t h h

F F

o o

r r k k 

### S S


## a a 

# g



# e e

C

r r e e

e e
k k 

# S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_CROWCKDN_OCT07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 2 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

OCTOBER 2007 FISHING REACHES:

SAGE CREEK AND CROW CREEK

DOWNSTREAM

0 1,000 2,000 500

Feet 

# Map Extent Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Surface Water Sampling Location

Fishing Reach

# SC3



# S 

# t t



# u u

m p C
r r 

# e e



# e e

k



# H 

# o 

# r s s 

# e C 

# r e e k

C C 

# o o 

# w C C 

# r r 

# e e 

# k S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_STUMPCK_OCT07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 3 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

OCTOBER 2007 FISHING REACHES:

STUMP CREEK

0 1,000 2,000 500

Feet 

# Map Extent

Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Surface Water Sampling Location

Fishing ReachM o n t p e l i e r C r e e k 

# MC1

Montpelier Reservoir 

# S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_MONTCK_OCT07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 4 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

OCTOBER 2007 FISHING REACHES:

MONTPELIER CREEK

0 1,000 2,000 500

Feet 

# Map Extent Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Surface Water Sampling Location

Fishing Reach

# CC150REDD CC350



# C C 

# r r



# o o

w w C C r r



# e e



# e e

k k



# W 

# a a



# r r

m C

r r e e

e e

k k 

# D D

e e

e e 

# r

r 

# C

C r r

e e

e e

k k S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_CROWCKUP_NOV07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 5 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

NOVEMBER 2007 FISHING REACHES:

CROW CREEK UPSTREAM

0 1,000 2,000 500

Feet 

# Map Extent Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Fishing Reach

# LSV2C

CC1A CC3A

CC1A-CC3A

C C r

r o o

w w 

# C C r r

e e 

# e

e k

k

S S

a a

g g

e e

C C

r r 

# e e



# e e

k k



# R R

o o



# c c



# k k

C

r r e e e e k k 

# S S

o o

u u t t h h

F F

o o

r r k k 

### S S


## a a 

# g



# e e

C

r r e e

e e
k k 

# S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_CROWCKDN_NOV07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 6 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

NOVEMBER 2007 FISHING REACHES:

SAGE CREEK AND CROW CREEK

DOWNSTREAM

0 1,000 2,000 500

Feet 

# Map Extent Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Fishing Reach

# SC3

SC1 S



# t u

m
p p



# C



# r r e e



# e e



# k k



# C o o

w w 

# C 

# r 

# e e 

# k H 

# o r r 

# s

s 

# e 

# C r e e

e k

k S:\GIS\ARCPRJ2\010109\PLT\BROWNREPRO\REFSMPLOCS_STUMPCK_NOV07.MXD

PRJ: 0442-004-900.70 DATE: NOV 19, 2008

BY: JPJ REV: 0 CHK: 

# J.R. SIMPLOT COMPANY

FIGURE 7 

# SMOKY CANYON MINE



# BROWN TROUT REPRODUCTION STUDY

NOVEMBER 2007 FISHING REACHES:

STUMP CREEK

0 1,000 2,000 500

Feet 

# Map Extent

Smoky
Canyon

Mine Caribou

Lincoln

Bear Lake Bonneville



# Legend

Fishing Reach

# APPENDIX C
Photographs of example deformities and Summary of the Deformity Rankings for Each SampleCSU

# Deformity Assessment

T he general scoring criteria were adopted from Holm et al. (2003) and included

assessments of craniofacial deformities, mostly of the head, eyes, and jaw, vertebral

deformities, fin deformities, and edema. The original publication showed pictures of some

deformities but others, particularly the intermediate categories were not illustrated or were

poorly described. More specific definitions for each of the assessment categories were

developed to give better repeatability and consistency across studies, and to aid others in

learning the range of deformities possible.

Deformities in each of the categories described above were given a score from 0-3, with 0

being a normal condition and 3 being the most deformed. Some range finding was

conducted over the first several samples to find background and severe levels of

deformities in each category. Initial samples were rescored as necessary to bring them

into compliance with the standards that were used throughout the assessment. In the

second batch of fish analyzed (~100 from 5 LSV2C sites), it was not always possible to

score each fish for each category due to the condition of the organism. Therefore, in

several cases no scoring was possible.

The protocol for assessing damage was to place several fish, head to the left, in a Petri

dish and examine them under a dissecting microscope and 10X magnification. The lateral

side was examined for spinal deformities (lordosis, kyphosis), appearance of the eye,

head and snout shape, edema, and fin deformities. The fish was turned ventrally to look

for mouth deformities and further spinal deformities (scoliosis), turned laterally again for

the same criteria as the other side, and then dorsally for issues associated with eyes, head

size, spinal deformities.

Craniofacial deformities included shortening of the jaw, snout, and missing or poorly

developed eye or eyes, and head shape abnormalities. A slightly shortened lower jaw (<=

1 lip width) received a 1, a shortened jaw = 2 lip widths or a slightly shortened and slightly

disfigured jaw = 2, and a flat lower jaw or much disfigured (non-functional) jaw = 3. An

assessment of fish independent of this study revealed that other brown trout of the same

size and developmental state did not have the slight deformity that was assessed as CF

=1 for the jaw (J). Thus, the CF = 1 score where the J was concerned were deemed real.

A slightly blunted snout (about 50% eye diameter, usually is > than that) = 1, very blunt or

flat = 2, deformed or bulbous = 3. Eye deformities were scored as one eye blind or poorly

pigmented or poorly developed =1, both poorly developed = 2, both blind = 3. Skulls that

were slightly bulbous (1/3 > normal) = 1, moderately bulbous (2/3 > normal) = 2, and

bulbous (1x or > than normal) = 3. Usually factors occurred together so a combination of

two “1” conditions = 2, three “1” conditions = 3, or a 1 and a 2 = 3, and so on. For

example, a deformed jaw and a blind eye = 2, two blind eyes = 2, but a badly deformed

jaw (= 2 alone) plus a blind eye (= 1 alone), = 3.

Skeletal deformities included any deformity of the vertebrae or spines. A slight bend of

less than 45 degrees (but > than body width off of straight) or a minor body constriction

(e.g. a tight rubber band about the body effect) was given a score of 1, 2 slight bends or

constrictions anywhere, or bend of > 45-90 degrees was scored a 2, and multi-directional

# bends > 90 degrees were given a 3. Bends caused by skeletal deformities were usually

d etectable from normal bending of the body during preservation (these fish were usually

well preserved, very straight) by presence of a slight or greater bump below the surface of

the epidermis on the outside of the bend. However, some fish with SD = 1 had just a very

slight bend in the range the deformity described but could be due to preservation or the

poor condition of the fish. This was sometimes especially true in larger fish, which may be

more muscular and undergo stronger contraction during preservation and thus, bend

slightly. A score “CF = 1” was a slight deformity, if at all. The scores of SD = 1 involving

kyphosis or lordosis were deemed real because that is an unusual preservation deformity.

Also, samples BKD 015 SU (i.e., extra fry from CC-150-015 at swim-up), LOW 008 SU

(i.e., extra fry from CC-350-008 at swim-up), and SC 003 SU (i.e., extra fry from SC-003 at

swim-up) were re-examined; most fish were very straight so some samples with higher SD

scores (e.g., PSU samples) were determined accurate. Thin fish difficult to score, and

often looked like they were underfed or starving.

Fin deformities included variation in fin or finfold morphology and a slightly smaller or

missing fin (in thin fish, the adipose fin was often absent, indicating fat absorption, not

uncommon and scored 1) or one with a bend or incomplete ray development (in older fish)

was given a 1, 2 fins damaged or malformed = 2, and > 2 fins malformed or if fins were

missing (except adipose) was = 3. Often fins were malformed associated with vertebral

deformities that did not permit proper development. Folded finfolds as a result of

preservation were not counted.

Edema was not originally scheduled for assessment because it was thought sometimes

not a teratogenic effect and may be transitory as fish develop. However, it was assessed

because it was common in one early sample and not others, and because it was

considered a condition that could affect emergence, mobility, and other factors that may

limit survival of fish in the wild. Edema was detected by an obvious swelling and fluid

buildup, usually abdominally, and ventrally, which often displaced the gut, and was usually

clear fluid that was slightly soft when touched with a blunt probe. The yolk, which was

present in some quantity in some study specimens, also created some swelling but was

typically yellowish, opaque, and small, and hard to the touch in preservation. Slight

edema = 1 was for a fish with up to 1X swelling of the normal body width or depth, up to 2x

= 2, and > 2x = 3.

A sample of 50 fish and a sample of 30 fish were scored twice, the same fish for each

batch but not necessarily the same order. This sample was characterized by a low

incidence of fin deformities (slow development) and a high incidence of jaw deformities

and blindness (SC 003 SU). Those cranio-facial traits are difficult to score because they

are additive, and subjective as to severity. Thus, the results may be a conservative view

of what score replicability should be like for other traits in other samples that are easier to

score.

Replicability of frequency of cranio-facial abnormalities was high among assessments at

50 and 52% in the first sample of 50 fish, and identical frequencies of 46.7 % in each

assessment for the sample of 30 fish. The cumulative sums of the scores were also quite

# close, but reflecting variability in scoring for all three categories of severity in each sample.

R eplicability of fin ray development assessments for both frequency and the sum of the

scores was identical in both samples.

Below we have included photographs of each of the deformities assessed described above, demonstrating scoring values of 0 – 3 for each of the deformities.Document No. 12699-001-500C March2009Photos 1 and 2: Example of normal brown trout eyes (left) and an example of a cranio-facial eye deformity with a score of 1 (right).Photos 3 and 4: Examples of cranio-facialeye deformities with a score of 3 (both).Document No. 12699-001-500CMarch2009Photos 1 and 2: Example of a normal brown trout jaw (left) and anexample of a cranio-facial jawdeformity with a score of 1 (right).Photos 3 and 4: Example of a cranio-facial jaw deformity with a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Example of a healthy brown trout fish (left) and an example of the spinal deformity constriction with a score of 1 (right).Photo 3: Example of the spinal deformity constriction with a score of 1.Document No. 12699-001-500CMarch 2009Photos 1 and 2: Example of a healthy brown trout fish (left) andan example of the skeletal deformity kyphosis with a score of1 (right).Photos 3 and 4: Example of the skeletal deformitykyphosis with a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Example of a healthy brown trout fish (left) and an example of the skeletal deformity lordosis with a score of1 (right).Photos 3 and 4: Example of the skeletal deformitylordosis with a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Example of a healthy brown trout fish (left) andan example of the spinal deformity scoliosis with a score of 1(right).Photos 3 and 4: Example of the spinal deformityscoliosis with a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Example of a healthy brown trout fish (left)and an example of a fin deformitywith a score of 1 (right).Photos 3 and 4: Example of a fin deformitywith a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Example of a healthy brown trout fish (left)and an example of abdominal edema with a score of 1 (right).Photos 3 and 4: Examples of abdominal edema with a score of 2 (left ) and 3 (right).Document No. 12699-001-500CMarch2009Photos 1 and 2: Example of a healthy brown trout fish (left) and an example of cranial edema with a score of 1 (right).Photos 3 and 4: Example of cranial edemawith a score of 2 (left ) and 3 (right).Document No. 12699-001-500C March2009Photos 1 and 2: Examples of brown trout with unusual deformities (both having two heads).Photos 3 and 4: Examples of unusual deformities.Page 1Deformity assessment of fry preserved after death during the BT parental study.filename: LSV2C def data.xls0 (normal)CF = craniofacial deformities1(slight/few)SD = vertebral deformities2 (mod/several)FD = fin deformities3 (severe/many)ED = edemaCountsCFTotalCFLocation Field Sample 0 1 2 3 Grand Total assessedLocation Field Sample 0 1 2 3 Grand TotalLSV2C 0030 3 83 12 98 98LSV2C 0030.0% 3.1% 84.7% 12.24% 100%0040 6 80 15 101 1010045.9% 79.2% 14.85% 100%0050 14 66 9 89 8900515.7% 74.2% 10.11% 100%0100 16 55 0 71 7101022.5% 77.5% 0.00% 100%0210 2 53 46 101 1010212.0% 52.5% 45.54% 100%SDTotalSDLocation Field Sample 0 1 2 3 Grand Total assessedLocation Field Sample 0 1 2 3 Grand TotalLSV2C 0030 56 20 6 82 82LSV2C 0030.0% 68.3% 24.4% 7.32% 100%0040 47 13 4 64 6400473.4% 20.3% 6.25% 100%0050 28 16 40 84 8400533.3% 19.0% 47.62% 100%0100 30 16 7 53 5301056.6% 30.2% 13.21% 100%0210 35 19 8 62 6202156.5% 30.6% 12.90% 100%FDTotalFDLocation Field Sample 0 1 2 3 Grand Total assessedLocation Field Sample 0 1 2 3 Grand TotalLSV2C 0030 0LSV2C 003#DIV/0! #DIV/0! #DIV/0! #DIV/0!00411 1004100.0% 0.0% 0.00% 100%0050 13 7 35 55 5500523.6% 12.7% 63.64% 100%0100 0010#DIV/0! #DIV/0! #DIV/0! #DIV/0!0210 9 5 0 14 1402164.3% 35.7% 0.00% 100%EDTotalEDLocation Field Sample 0 1 2 3 Grand Total assessedLocation Field Sample 0 1 2 3 Grand TotalLSV2C 0030 47 30 7 84 84LSV2C 00356.0% 35.7% 8.33% 100%0040 57 28 6 91 9100462.6% 30.8% 6.59% 100%0050 40 13 5 58 5800569.0% 22.4% 8.62% 100%0100 16 19 10 45 4501035.6% 42.2% 22.22% 100%0210 62 19 1 82 8202175.6% 23.2% 1.22% 100%Note: scoring criteria were not possible for all organismsdue to the poor physical condtion of some samples. For these samples, no value was included.No organisms scored a "0" on any of the different assessments (i.e., CF, SD, FD, ED)

# Page 2 of 5

Deformity assessment results for brown trout in reproductive success study

Vaues represent the number of fish (at swimup and at test termination) in each scoring criterion (i.e., 0 - 3).

Se e below for a definition of scoring criteria.

filename: deformity data.xls



# Count of Fish # Craniofacial Deformities (CF)



# Location Field Sample ID 0 1 2 3 Grand Total

CC-150 009 136 1 2 3 142

011 114 150 2 266

012 191 86 4 1 282

013 183 31 28 68 310

015 231 207 5 2 445

016 20 2 1 23

017 108 54 1 163

018 288 193 2 3 486

020 506 52 558

CC-150 Total 1777 776 44 78 2675

CC-350 006 228 122 22 14 386

007 102 12 11 6 131

008 315 8 5 10 338

CC-350 Total 645 142 38 30 855

LSV2C 002 531 13 544

003 3 83 12 98

0 04 63 6 80 15 164

0 05 27 27 75 9 138

0 08 165 24 5 194

010 16 55 71

0 12 511 39 3 1 554

016 495 34 1 530

017 122 16 10 2 150

019 302 79 8 1 390

020 257 36 3 296

021 47 13 57 53 170

L SV2C Total 2520 306 380 93 3299

SC 001 96 14 4 1 115

002 104 6 1 2 113

003 174 37 55 36 302

004 69 26 26 19 140

005 39 3 42

006 519 2 6 8 535

007 119 11 6 1 137

008 339 12 3 5 359

SC Total 1459 111 101 72 1743

SPC 001 490 75 2 1 568

003 448 91 6 545

005 476 82 2 1 561

006 475 77 3 1 556

SPC Total 1889 325 13 3 2230

Grand Total 8290 1619 239 194 10342

Craniofacial deformities included shortening of the jaw, snout, and missing or poorly developed eye or eyes, and head shape

abnormailities. A slightly shortened lower jaw (<= 1 lip width) received a 1, a shortened jaw = 2 lip widths or a slightly shortened

and slightly disfigured jaw = 2, and a flat lower jaw or much disfigured (non-functional) jaw = 3. An assessment of fish

independent of this study revealed that other brown trout of the same size and developmental state did not have the slight deformity

that was assessed as CF =1 for the jaw (J). Thus, the CF = 1 score where the J was concerned were deemed real. A slightly

blunted snout (about 50% eye diameter, usually is > than that) = 1, very blunt or flat = 2, deformed or bulbous = 3. Eye deformities

were scored as one eye blind or poorly pigmented or poorly developed =1, both poorly developed = 2, both blind = 3. Skulls that

were slightly bulbous (1/3 > normal) = 1, moderately bulbous (2/3 > normal) = 2, and bulbous (1x or > than normal) = 3. Usually

factors occurred together so a combination of two “1” conditions = 2, three “1” conditions = 3, or a 1 and a 2 = 3, and so on. For

example, a deformed jaw and a blind eye = 2, two blind eyes = 2, but a badly deformed jaw (= 2 alone) plus a blind eye (= 1 alone), = 3.

# Page 3 of 5

Deformity assessment results for brown trout in reproductive success study

Vaues represent the number of fish (at swimup and at test termination) in each scoring criterion (i.e., 0 - 3).

S ee below for a definition of scoring criteria.

filename: deformity data.xls



# Count of Fish # Skeletal Deformities (SD)



# Location Field Sample ID 0 1 2 3 Grand Total

CC-150 009 109 28 3 2 142

011 213 50 3 266

012 237 42 3 282

013 214 81 11 4 310

015 402 33 8 2 445

016 13 10 23

017 150 11 2 163

018 353 121 11 1 486

020 499 44 15 558

CC-150 Total 2190 420 56 9 2675

CC-350 006 198 117 43 28 386

007 83 22 20 6 131

008 284 43 7 4 338

CC-350 Total 565 182 70 38 855

LSV2C 002 499 38 7 544

003 56 20 6 82

0 04 20 83 20 4 127

0 05 17 44 29 43 133

0 08 173 19 2 194

010 30 16 7 53

0 12 235 306 13 554

016 486 41 3 530

017 138 10 2 150

019 341 46 2 1 390

020 274 17 4 1 296

021 20 71 32 8 131

L SV2C Total 2203 761 148 72 3184

SC 001 79 28 7 1 115

002 75 32 3 3 113

003 260 39 3 302

004 99 28 6 7 140

005 25 17 42

006 486 42 6 1 535

007 105 23 4 5 137

008 291 47 8 13 359

SC Total 1420 256 37 30 1743

SPC 001 493 62 9 4 568

003 457 64 21 3 545

005 479 65 12 5 561

006 488 41 22 5 556

SPC Total 1917 232 64 17 2230

Grand Total 8295 1655 291 101 10342

Skeletal deformities included any deformity of the vertebrae or spines. A slight bend of less than 45 degrees (but > than body width off

of straight) or a minor body constriction (e.g. a tight rubberband about the body effect) was given a score of 1, 2 slight bends or

constrictions anywhere, or bend of > 45-90 degrees was scored a 2, and multi-directional bends > 90 degrees were given a 3. Bends

caused by skeletal deformities were usually detectable from normal bending of the body during preservation (these fish were usually

well preserved, very straight) by presence of a slight or greater bump below the surface of the epidermis on the outside of the bend.

However, some fish with SD = 1 had just a very slight bend in the range the deformity described but could be due to preservation or

the poor condition of the fish. This was sometimes especially true in larger fish, which may be more muscular and undergo stronger

contraction during preservation and thus, bend slightly. A score “CF = 1” was a slight deformity, if at all. The scores of SD = 1

involving kyphosis or lordosis were deemed real because that is an unusual preservation deformity. Some samples were re-examined;

most fish were very straight so some samples with higher SD scores (e.g., PSU samples) were determined accurate.

# Page 4 of 5

Deformity assessment results for brown trout in reproductive success study

Vaues represent the number of fish (at swimup and at test termination) in each scoring criterion (i.e., 0 - 3).

Se e below for a definition of scoring criteria.

filename: deformity data.xls



# Count of Fish # Fin Deformities (FD)



# Location Field Sample ID 0 1 2 3 Grand Total

CC-150 009 137 2 1 2 142

011 266 266

012 279 1 2 282

013 287 17 4 2 310

015 437 3 4 1 445

016 23 23

017 162 1 163

018 483 3 486

020 549 9 558

CC-150 Total 2623 36 9 7 2675

CC-350 006 325 16 16 29 386

007 95 10 18 8 131

008 303 25 7 3 338

CC-350 Total 723 51 41 40 855

LSV2C 002 528 15 1 544

003 0

004 48 15 1 6 4

005 39 17 11 37 104

0 08 194 194

010 0

012 544 9 1 554

016 485 45 530

017 144 4 2 150

019 390 390

020 292 1 3 296

021 27 51 5 83

L SV2C Total 2691 156 20 42 2909

SC 001 102 7 5 1 115

002 103 6 4 113

003 280 21 1 302

004 113 10 13 4 140

005 42 42

006 501 21 7 6 535

007 114 11 7 5 137

008 343 4 3 9 359

SC Total 1598 80 39 26 1743

SPC 001 542 11 10 5 568

003 524 8 7 6 545

005 533 16 4 8 561

006 529 11 7 9 556

SPC Total 2128 46 28 28 2230

Grand Total 9763 346 125 108 10342

Fin deformities included variation in fin or finfold morphology and a slightly smaller or missing fin (in thin fish, the adipose fin was often

absent, indicating fat absorption, not uncommon and scored 1) or one with a bend or incomplete ray development (in older fish) was

given a 1, 2 fins damaged or malformed = 2, and > 2 fins malformed or if fins were missing (except adipose) was = 3. Often fins were

malformed associated with vertebral deformities that did not permit proper development. Folded finfolds as a result of preservation

were not counted.

# Page 5 of 5

Deformity assessment results for brown trout in reproductive success study

Vaues represent the number of fish (at swimup and at test termination) in each scoring criterion (i.e., 0 - 3).

Se e below for a definition of scoring criteria.

filename: deformity data.xls



# Count of Fish # Edema Deformities (ED)



# Location Field Sample ID 0 1 2 3 Grand Total

CC-150 009 141 1 142

011 266 266

012 282 282

013 308 2 310

015 445 445

016 23 23

017 163 163

018 485 1 486

020 558 558

CC-150 Total 2671 3 1 2675

CC-350 006 382 3 1 386

007 126 3 2 131

008 337 1 338

CC-350 Total 845 7 3 855

LSV2C 002 541 3 544

003 47 30 7 84

0 04 63 57 28 6 154

0 05 42 46 14 5 107

0 08 180 6 8 194

010 16 19 10 45

0 12 554 554

016 530 530

017 135 9 5 1 150

019 381 8 1 390

020 296 296

021 69 62 19 1 151

L SV2C Total 2791 254 124 30 3199

SC 001 114 1 115

002 113 113

003 302 302

004 139 1 140

005 42 42

006 534 1 535

007 137 137

008 359 359

SC Total 1740 3 1743

SPC 001 565 3 568

003 539 4 2 545

005 558 3 561

006 553 1 1 1 556

SPC Total 2215 11 3 1 2230

Grand Total 10262 56 22 2 10342

Edema was not originally scheduled for assessment because it was thought sometimes not a teratogenic effect and may be transitory

as fish develop. However, it was assessed because it was common in one early sample and not others, and because it was thought

a condition that could affect emergence, mobility, and other factors that may limit survival of fish in the wild. Edema was detected by an

obvious swelling and fluid buildup, usually abdominally, and ventrally, which often displaced the gut, and was usually clear fluid that

was slightly soft when touched with a blunt probe. The yolk, which was present in some quantity in some study specimens, also

created some swelling but was typically yellowish, opaque, and small, and hard to the touch in preservation. Slight edema = 1 was

for a fish with up to 1X swelling of the normal body width or depth, up to 2x = 2, and > 2x = 3.Figure1Cranio‐FacialFrequency00.10.20.30.40.50.60.70.80.91SPC001SPC005SC 001 SC 003 SC 005 SC 007 CC-150009CC-150012CC-150015CC-150017CC-150020CC-350007LSV2C002LSV2C004LSV2C008LSV2C016LSV2C019LSV2C021

Percentage of Sample0(normal)1(slight/few)2(mod/several)3(severe/many)(blank)NAFigure2SkeletalDeformityFrequency00.10.20.30.40.50.60.70.80.91SPC001SPC005SC 001 SC 003 SC 005 SC 007 CC-150009CC-150012CC-150015CC-150017CC-150020CC-350007LSV2C002LSV2C004LSV2C008LSV2C016LSV2C019LSV2C021

Percentage of Sample0(normal)1(slight/few)2(mod/several)3(severe/many)(blank)Figure3FinorFinfoldDeformityFrequency00.10.20.30.40.50.60.70.80.91SPC001SPC005SC 001 SC 003 SC 005 SC 007 CC-150009CC-150012CC-150015CC-150017CC-150020CC-350007LSV2C002LSV2C004LSV2C008LSV2C016LSV2C019LSV2C021

Percentage of Sample0(normal)1(slight/few)2(mod/several)3(severe/many)(blank)Figure4EdematousTissueFrequency00.10.20.30.40.50.60.70.80.91SPC001SPC005SC 001 SC 003 SC 005 SC 007 CC-150009CC-150012CC-150015CC-150017CC-150020CC-350007LSV2C002LSV2C004LSV2C008LSV2C016LSV2C019LSV2C021

Percentage of Sample0(normal)1(slight/few)2(mod/several)3(severe/many)(blank)