DISCLAIMER 

This report is the property of Clariant. 

Its content is confidential and not for general 

release. It is not allowed to cite and reveal the report or its 

content to a third party without prior written approval of 

Clariant. 

EXPERT STATEMENT 

Leuco Sulfur Black 1 

3tj515F5 

haria nTh .... 

Calculation of the Partition Coefficient (n-Octanol I Water) 

Study Director: 

Test Facility: 

Sponsor: 

Dr Martin Kiihne 

Harlan Laboratories Ltd. 

Zelgliweg 1 

4452 Itingen I Switzerland 

Clariant Products (Switzerland) Ltd. 

Rothausstrasse 61 

4132 Muttenz 1 I Switzerland 

Study Identification: Harlan Laboratories Study C88384 

Version: Final 

Study Completion Date: April14, 2010 

Page 1 of8 

CONTAINS NO CP 
Harlan Laboratories Study C88384 

Leuco Sulfur Black 1 

CONTENTS 

Expert Statement Page2 

CONTENTS .................................................................................................................................... 2 

SIGNATURE .................................................................................................................................. 3 

PREFACE ....................................................................................................................................... 4 

General Information ............................................................................................................... 4 

Responsibilities ...................................................................................................................... 4 

Schedule ................................................................................................................................. 4 

Archiving ............................................................................................................................... 4 

1 PURPOSE .............................................................................................................................. 5 

2 TEST ITEM ........................................................................................................................... 6 

3 RESULTS AND DISCUSSION ............................................................................................ 7 

4 CONCLUSION ...................................................................................................................... 8 
Harlan Laboratories Study C88384 

Leuco Sulfur Black 1 

SIGNATURE 

Study Director: 

Expert Statement Page 3 

Dr Martin KUhne 
Harlan Laboratories Study C88384 

Leuco Sulfur Black I 

PREFACE 

General Information 

Test Item: 

Study Title: 

Sponsor: 

Study Monitor: 

Test Facility: 

Responsibilities 

Study Director/Scientific Author: 

Schedule 

Date of Issue: 

Archiving 

Expert Statement Page4 

Leuco Sulfur Black 1 

Calculation of the Partition Coefficient (n-Octanol I 

Water) 

Clariant Products (Switzerland) Ltd. 

Rothausstrasse 61 

4132 Muttenz 1 I Switzerland 

Dr Bruno Kunz 

Harlan Laboratories Ltd. 

Zelgliweg 1 

4452 ltingen I Switzerland 

Dr Martin Kiihne 

April14, 2010 

Harlan Laboratories Ltd., 4452 Itingen/Switzerland will retain the expert statement of the present 

study for at least ten years. 

No data will be discarded without the Sponsor's written consent. 
Harlan Laboratories Study C88384 

Leuco Sulfur Black 1 

1 PURPOSE 

Expert Statement PageS 

In this study the partition coefficient (n-octanol!water) of Leuco Sulfur Black 1 should be 

estimated. 

The partition coefficient provides a rational basis for an assessment of the potential 

bioaccumulation of the test item. 
Harlan Laboratories Study C88384 

Leuco Sulfur Black 1 

2 TESTITEM 

Expert Statement 

Data of test item as supplied by the Sponsor. 

Identification: 

CAS Nr.: 

Description: 

Batch Number: 

Composition: 

Stability of the test item: 

Stability of the test item dilution: 

Expiry date: 

Storage Conditions: 

Leuco Sulfur Black 1 

66241-11-0 

Thixotrophic, black, wet presscake 

ESA0007595 

Leuco Sulfur Black 1: 52.75% (calculated by 

amount of by-compounds) 

2,4-Dinitrochlorobenzene: < 20 ppm 

2,4-Dinitrophenole: < 20 ppm 

Sulfate: 970 ppm 

Chloride: 750 ppm 

Sodium: 1.3% (w/w) 

Water: 45.75% (w/w) by Karl-Fischer titration 

Stable under storage conditions 

> 4 h in water 

July 11, 2011 

At room temperature (range 20 +!- 5 °C), light 

protected 

Page6 
Harlan Laboratories Study C88384 

Leuco Sulfur Black I 

Expert Statement 

3 RESULTS AND DISCUSSION 

Page? 

The partition coefficient (Pow) is defined as the ratio of the solubility of the test item in octanol 

and in water: 

p = C n-octanol 

ow cwater 

No chromatographic method is applicable to determine the solubility of Leuco Sulfur Black 1 in 

n-octanol. 

Although it is possible to estimate the water solubility of Leuco Sulfur Black 1 (62.4 mg/L as 

determined in Harlan Laboratories Study C75560) by measurement of the Total Carbon (TC) 

content this analytical method is inapplicable in case of n-octanol as a solvent. 

Therefore it is impossible to determine the log Pow of Leuco Sulfur Black 1 according to the 

guideline. 

' .;> ~ ..... ' ~· ...,, . ·dWv t~, 

.:=:~ll·t· 
Harlan Laboratories Study C88384 

Leuco Sulfur Black 1 

4 CONCLUSION 

Expert Statement Page 8 

No chromatographic method is available to estimate the solubility of Leuco Sulfur Black I in noctanol. Therefore the log Pow cannot be determined according to the guideline. 

CONTAINS NO CBI 

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