Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

1

Data Requirement: PMRA DATA CODE: 9.5.2.1; 9.5.2.2; 9.5.2.3; 9.5.2.4 

EPA DP Barcode: Not applicable 

OECD Data Point: IIA 8.2.1 and IIA 8.2.1.2 

EPA Guideline: 850.1075 

Test material: Technical and Formulation Purity (%): TGAI 98% and EP 48%

Common name: Fluridone 

Chemical name: 1-methyl-3-phenyl-5-(α,α,α-trifluoro-m-tolyl)-4-pyridone 

IUPAC: 1-methyl-3-phenyl-5-(α,α,α-trifluoro-m-tolyl)-4-pyridone 

CAS name: 1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone 

CAS No.: 59756-60-4 

Synonyms: 

Primary Reviewer: Katrina White Date: February 24, 2010 

USEPA/OPPTS/OPP/ERBII/Biologist 

Secondary Reviewer(s): Donna M. Randall Date: February 24, 2010 

USEPA/OPPTS/OPP/ERBII/Senior Effects Scientist 

Company Code: [For PMRA]

Active Code: [For PMRA] 

Use Site Category: [For PMRA]

EPA PC Code: 112900 

CITATION: U.S. Fish and Wildlife Service. (1982). The Toxicity of Fluridone (El-171, Compound 

112371) and of Sonar AS, an Aqueous Suspension Formulation of Fluridone, to Aquatic Invertebrates 

and Fish in Acute and Chronic Studies. (Unpublished study received Jun 10, 1982 under 1471-126; 

prepared in cooperation with U.S. Fish and Wildlife Service, Columbia National Fisheries Research 

Laboratory, submitted by Elanco Products Co., Div. of Eli Lilly and Co., Indianapolis, IN; 

CDL:070932-F). MRID 000103297 

The acute endpoints from this study are identical to those reported in: Mayer, F., and M. Ellersieck 

(M&E) (1986). Manual of Acute Toxicity: Interpretation and Data Base for 410 Chemicals and 66 

Species of Freshwater Animals. US Fish & Wildlife Service, Resource Publication 160. 579 p. 

MRID 40098001 

Raw data were obtained from the Federal Lab Data submitted to the EFED. 

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data 

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to fish. It is not 

intended to prescribe conditions to any external party for conducting this study nor to establish absolute criteria 

regarding the assessment of whether the study is scientifically sound and whether the study satisfies any applicable 

data requirements. Reviewers are expected to review and to determine for each study, on a case-by-case basis, 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

2

whether it is scientifically sound and provides sufficient information to satisfy applicable data requirements. 

Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that meet all of the 

conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of factors related 

to the test methodology and results in determining the acceptability of the study. 

The following template illustrates the critical elements and the level of detail required by the PMRA and the EPA in 

a study review of the acute toxicity to fish. It is not meant to be a "fill in the blanks" exercise although it may be 

appropriate in some cases. The standard format of the template should, however, be followed in preparing the study 

review. 

EXECUTIVE SUMMARY:

In 96-hr acute toxicity tests, rainbow trout (Oncorhynchus mykiss formerly Salmo gairdneri), bluegill 

sunfish (Lepomis macrochirus), channel catfish (Ictalurus punctatus), and fathead minnnow (Pimephales 

promelas) were exposed to technical grade active ingredient (TGAI) and an end-use product (EP), Sonar 

4 AS. Rainbow trout and channel catfish were exposed at two hardness levels (40 and 320 mg/L as 

calcium carbonate,CaCO3), three temperatures (7, 12, and 17 for rainbow trout and 7, 12, and 22 for 

channel catfish), and three pH (6.5, 7.5, and 8.5). Nominal concentrations in the tests varied but were 

typically 0 (negative control and solvent control (acetone) for TGAI studies), 1.8, 3.2, 5.6, 10, 18, and 32 

mg a.i./L under static conditions, additional nominal concentrations tested included 0.56, 1.0, 56, and 100 

mg a.i./L. 

Except for one test, fluridone concentrations were measured twice in the tests (test initiation and 

termination); however, the measured concentrations were only summarized in the USFWS (1982) study 

report. In some tests, the average measured concentrations were reportedly ≥79% of nominal. In other 

tests, average concentrations were <79% of nominal or samples were lost. In M&E (1986) and the 

USFWS (1982) study report results are based on nominal. However, use of nominal concentrations to 

represent soluble fluridone exposures in the case of no analytical confirmatory data or with conditions of 

poor agreement with nominal concentrations potentially underestimates the toxicity of fluridone. 

Fluridone has a short degradation rate and all tests included concentrations of fluridone above its 

solubility; one study actually reported insoluble fluridone at concentrations used in all tests. Therefore, if 

no sample analysis was available, the reviewer excluded the treatments above solubility from calculating 

the LC50 if mortality levels allowed, and all these results were identified as based on total fluridone. Even 

where there was indication of agreement with nominal concentrations, the reviewer did not include 

concentrations above solubility if mortality was high in concentrations below solubility, in calculating the 

LC50 values. If mortality occurred primarily above solubility, the results were annotated as total because 

there is a high degree of uncertainty of soluble fluridone concentrations without looking at the specific 

fluridone treatment concentrations. 

The lowest quantitative TGAI 96-h LC50 for rainbow trout was 5.1 mg a.i./L (mean measured). The 

lowest quantitative TGAI 96-hr LC50 for channel catfish was 7.5 mg a.i./L (based on nominal 

concentration, however anlyatical analysis indicates that nominal concentrations were reliable). An 

endpoint could be calculated for the EP study for the rainbow trout, the endpoint is slightly lower (96-hr 

LC50 = 4.3 mg a.i./L measured; 95% C.I. 2.9 - 6.3) than the TGAI but the confidence interval overlaps 

with that of the TGAI. This suggests that the formulation is not more toxic to rainbow trout. The lowest 

quantitative 96-hr LC50 for bluegill sunfish was 6.5 mg a.i./L (mean measured, 95% Confidence interval 

5.5 -7.7) and for the fathead minnow 8.3 mg a.i./L (measured, 95% C.I. 6.7 – 10.2), both of these were for 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

3

the formulation. Based on test results classified as acceptable, fluridone would be classified as 

moderately toxic (96-h LC50 >1-10 mg/L) to rainbow trout, channel catfish, bluegill sunfish, and fathead 

minnow in accordance with EFED’s toxicity classification system. 

No replicates were available to determine NOAEC values statistically. Sublethal effects of immobility, 

loss of equilibrium, and surfacing were observed in rainbow trout exposed to nominal concentrations of 

3.2, 3.2, and 5.6 mg a.i./L, respectively. Immobility, loss of equilibrium, and surfacing were also 

observed in channel catfish, bluegill sunfish, and fathead minnow but at higher nominal concentrations. 

Confidence intervals of 96-hr LC50s overlapped for toxicity tests for the rainbow trout performed at pH of 

6.5, 7.5, and 8.5 indicating that pH did not influence the toxicity of fluridone. Confidence intervals of 

qualitative endpoints for the rainbow trout performed at different hardness (40 and 320 mg/L CaCO3) and 

temperatures (7, 12, and 17o

C). This suggests that hardness and temperature also did not influence the 

toxicity of fluridone. See Table 17 for results for each test. 

In several tests, only binomial 96-hr LC50 values were available because partial mortality was only 

observed at one treatment level or at no treatment levels below solubility. The slope of the dose-response 

curve for fluridone appears to be very steep indicating that the distance between the threshold 

concentration that produces effects and the concentration that will result in 100% mortality is very small. 

Additionally, the threshold of effects appears to be near the level of solubility for fathead minnow and 

channel catfish. This results in some difficulty in measuring the median lethal concentration as the 

exposure concentration must be near solubility to observe partial mortality above and below the LC50. 

Many of the tests had measured concentrations at <79% nominal. This was reportly due to storing 

samples too long and poor handling of the samples. The short aqueous photolysis half-life of fluridone 

indicates that it will be common for fluridone to degrade over the test duration. Concentrations must be 

measured in order to verify that the exposure concentration was constant over time. 

Table 1 summarizes the results of fluridone tests classified as acceptable. Table 17 summarizes all the 

study results and classifications. Rainbow trout test numbers 13, 18, 19, 20, 21, are classified as 

acceptable and satisfy the data requirement for a TGAI study conducted with a cold water species. 

Channel catfish test number 32 is classified as acceptable and satisfies the data requirement for a TGAI 

study conducted with a warm water species. Observation 14, 24, and 36 for the rainbow trout, channel 

catfish, and bluegill, respectively, are classified as acceptable EP studies for Sonar 4 AS. It is not known 

if Sonar 4 AS is representative of an EP currently on the market. 

Results Synopsis

Test Organism Size (mean wet weight and/or length) and Age: 

 Rainbow Trout fingerlings (0.7 – 1.2 g) 

 Bluegill Sunfish fingerlings (0.5 g) 

 Channel Catfish fingerlings ( 0.4 – 0.9g) 

 Fathead Minnnow fingerlings (0.8 g) 

Test Type (Flow-through, Static, Static Renewal): Static 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

4

Table 1. Summary of the tests classified as acceptable. 

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L

(95% CI)/ 

[Slope, (95% CI)] 

Basis/ 

Statistical 

Method 

Study Classification/ 

Comments 

13 Rainbow 

Trout, 

0.7 g 

TGAI 12 o

C, 7.3 pH, 

40, 35 

5.1 

(4.1-6.4)/ 

[N/A] 

Measured/ 

Binomial 

Acceptable/ 

Included only reported measured 

values to calculate endpoint 

14 Rainbow 

Trout, 

1.1 g 

EP 12 o

C, 7.1 pH, 

40, 35 

4.3 

(2.9-6.3)/ 

[N/A] 

Measured/ 

Binomial 

Acceptable/ 

Included only reported imeasured 

values to calculate endpoint 

18 Rainbow 

Trout, 

0.98 g 

TGAI 12 o

C, 6.5 pH, 

40, 35 

5.7 

(4.5-7.4)/ 

[6.7, 3.0-10.3] 

Nominal*/ 

Probit 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

19 Rainbow 

Trout, 

0.98 g 

TGAI 12 o

C, 7.5 pH, 

40, 35 

5.6 

(3.2-10.0)/ 

[N/A] 

Nominal*/ 

Binomial 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

20 Rainbow 

Trout, 

0.98 g 

TGAI 12 o

C, 8.5 pH, 

 40, 35 

7.0 

(5.6-10.0)/ 

[N/A] 

Nominal*/ 

Binomial 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

21 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 8.1 pH, 

40, 235 

6.7 

(5.3-8.5)/ 

[7.7, 3.1-12.2] 

Nominal*/ 

Probit 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

22 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 8.1 pH, 

 320, 235 

8.0 

(5.6-10.0)/ 

[N/A] 

Nominal*/ 

Binomial 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

24 Fathead 

Minnow, 

0.78 g 

EP 22 o

C, 7.1 pH, 

40, 35 

8.3 

(6.7 – 10.2)/ 

[N/A] 

Measured/ 

Binomial 

Acceptable/ 

Study authors endpoint from 

measured values 

32 Channel 

Catfish, 0.4 

g 

TGAI 22 o

C, 8.5 pH, 

 42, 35 

7.5 

(6.1-9.2) 

[10.2, (4.3-16.1)] 

Nominal*/ 

Probit 

Acceptable/ 

Included only nominal 

concentrations below the solubility 

limit. 

36 Bluegill, 

0.5 g 

EP 22 oC, 7.1 pH, 

40, 35 

6.5 

(5.5 – 7.7)/ 

[N/C] 

Measured/ 

LitchfieldWilcoxon 

Acceptable/ 

Measured concentrations were not 

provided but the 96-hr LC50 based 

on measured concentrations was 

reported in Appendix F of the study 

report. 

Obs # = M&E (1986) study observation number for fluridone; EP=End-use product; TGAI=Technical Grade Active Ingredient; 

CI=confidence interval; N/A = not applicable 

*Nominal exposure levels used in determination of endpoint but nominal concentrations were confirmed analytically (fluridone 

concentrations average > 79 percent of nominal concentrations). 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

5

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The protocol was based on methods recommended by the 

Committee on Methods for Toxicity Tests with Aquatic 

Organisms (1975). Methods for acute toxicity tests with fish, 

macroinvertebrates, and amphibians. U.S. EPA. Ecol. Res. 

Serv. EPA-660/3-75-009, 61p, and standard protocols of the 

National Fisheries Research Laboratory 

Study Deficiencies As Compared to draft OPPTS 850.1075 Fish Acute Toxicity Guideline: 

1. The physiochemical properties of the test material were not reported. However the 

information was obtained from other sources. 

2. A GLP statement was not provided but protocols for recording and documenting test results, 

study investigators, and similar requirements to GLP were in place. 

3. There was only one replicate with 10 individuals at each treatment level, but this did not 

affect the determination of the EC50, confidence in estimates may be reduced. 

4. Total organic carbon and particulate matter concentrations were not reported but distilled 

water, which should result in particulate and organic carbon levels to be within acceptable 

levels, was used for reconstituted water. The well water is assumed to have been within 1975 

guideline standards which was cited as being followed. 

5. Dissolved oxygen (DO) concentrations were not reported but control mortality was within 

acceptable levels, indicating DO was not likely an issue. 

6. Analytical results for some studies indicate that their nominal concentrations are not 

appropriate for use as exposure levels. In some cases analytical results were considered 

unusable and in others the results were used to calculate study endpoints based on measured 

levels, these were provided in an appendix whereas the main body of the study reported 

results based on nominal. In either case the actual analytical results were not included in the 

study report. For the specific studies affected, these deviations significantly affect the 

accuracy and reliability of the concentration-response relationship and the measurement 

endpoint value. 

Except for the analytical results for some studies, these deviations are considered minor for the reasons 

provided and do not affect the scientific soundness of this study. Specific deficiencies associated with 

analytical results are discussed below. 

Other deficiencies specific to each observation/test. 

Observation # 15, 16, 17, 25, 26 and 7/17/80 Channel catfish study: These tests were classified as 

supplemental-qualitative because measured concentrations of soluble fluridone are not available. This is 

a significant deviation from guidance for low solubility test materials tested near or above their solubility 

and for test materials that have short half-lifes, such as fluridone’s photodegradation half-life of 0.9-2.3 

days, which potentially impact its stability during the test. If fluridone has degraded or become 

unavailable due to insolubility, the use of nominal concentrations in this case will make fluridone appear 

less toxic than it really is (i.e., LC50 values calculated using nominal concentrations will be higher than if 

they were based on measured, soluble fluridone concentrations). In a risk assessment use of these values 

will likely underestimate risk. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

6

Observation # 23, 27, 28, 29, 30, 31, 33, 34, and 35: While the study reported that fluridone 

concentration average was >79% of nominal for these studies, it is highly unlikely that the nominal 

concentrations near and above solubility are representative of soluble fluridone concentrations. For these 

studies knowing the actual measured concentrations for each treatment level is important because effects 

primarily occured at treatments above known solubility of fluridone (12.3 ppm in distilled water). Some 

specific treatment measurements, rather than averages, provided in the report for 10, 18, and 56 ppm 

nominal treatments were 63 to 64% (obs 13, 14), 37% (obs 24), and 18% (obs 24) of nominal, 

respectively. Additionally one study reported insoluble fluridone present at nominal 18, 32, and 56 ppm 

fluridone (obs 25) treatments but identified that fluridone average concentrations > 79% of nominal 

indicating that specific treatments may have had issues. Therefore, the lack of the actual analytical results 

for these results introduces a high degree of uncertainty in the representation of nominal concentrations 

for concentrations near and above solubility even though the average concentration for a study is reported 

as >79% of nominal, as individual treatments may be well below this. Lack of raw data which allows an 

independent evaluation of the soluble concentration at which effects occur under these circumstances is 

considered a significant deviation. If fluridone has become unavailable due to insolubility, the use of 

nominal concentrations in this case will make fluridone appear less toxic than it really is (i.e., LC50 values 

calculated using nominal concentrations will be higher than if they were based on measured, soluble 

fluridone concentrations). In a risk assessment use of these values will likely underestimate risk. These 

studies are potentially repairable if the raw measured fluridone concentrations for each treatment and 

information about the filter pore size were submitted.

COMPLIANCE: A statement of compliance with GLP procedures was not 

provided. The studies were completed prior to the GLP 

requirement. However, protocols for recording and 

documenting test results, study investigators, 

measurement of batch test material purity, measurement 

of test material during the study, and similar 

requirements to GLP appear to have been in place. 

A. MATERIALS:

1. Test Material TGAI: Technical grade Fluridone (purity: 98% active) 

 EP: Sonar 4 AS, 4 lb a.i./gallon field formulation (purity: 48% fluridone) 

Description: TGAI: powder 

 EP: liquid

Lot No./Batch No. : TGAI: D36-Y93-107 (Page 389) 

 EP: X-36153 (Page 388) 

Purity: TGAI: 98% 

 EP: 48% 

 Stability of Compound 

 Under Test Conditions: Except for the 7/17/80 channel catfish study, sampling was conducted 

on all treatment levels at 2 sampling intervals (p 342 of study report), this would be consistent with 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

7

sampling at test initiation and termination. Raw fluridone concentration data were not provided but the 

results were summarized on Page 405 of the study report and summarized below in Table 2. As fluridone 

is expected to degrade rapidly in the presence of light (aqueous photolysis half-life = 0.9-2.3 days; MRID 

00103326) and tests included nominal concentrations near and above known fluridone solubility, 

measured concentrations of soluble fluridone concentrations are necessary for the TGAI studies to 

document that exposure to soluble fluridone concentrations were stable throughout the duration of the 

study. For studies with the EP measurement of the soluble fluridone concentrations are necessary to 

document exposure to bioavailable fluridone. 

Table 2. Summary of analytical measurements of the test concentrations and stability conclusions. 

Test Species 

Observation Number 

Summary of Analytical Results* Are the exposure concentrations reliable?

Observation 13, 14, 24 Analtyical results indicate that actual 

fluridone concentrations averaged 

<79% nominal. Measured values 

were reported for highest treatment 

with no mortality and lowest 

treatment with 100% mortality at 96 

hours. 

Nominal values are not reliable but 

measured values were provided which 

allow calculation of endpoint using 

binomial method. 

Observation 23, 27, 28, 

29, 30, 31 

Measured concentrations averaged 

≥79% of nominal concentrations. 

While the averaged fluridone concentrations 

indicate reliability, specific exposure 

concentrations above solubility are unlikely 

to have soluble concentrations near nominal 

(as evidenced by values that were reported 

for obs 13, 14, 24) and insoluble fluridone 

reported in obs 25. For these studies effects 

occurred primarily above solubility. 

Without actual reported analytical results 

for these specific treatments, nominal 

results are considered highly uncertain as 

representative of soluble exposure 

concentrations and are qualified either on a 

total basis or if no mortality occurred at the 

highest treatment below solubility, it is 

assigned as a suitable limit test. 

Observation 15, 16, 17, 

25, 26, and Channel 

catfish study not 

reported in M&E 

(1986) 

Analysis of test material was not 

performed. Water samples were not 

collected (channel catfish), lost (obs 

25, 26), or samples were stored 

longer than 6 months and the sample 

shipment was received in poor 

condition (obs 15, 16, 17). 

Nominal concentrations are not considered 

a reliable quantitative representation of 

soluble exposure concentrations throughout 

the study, due to potential for degradation 

and insolubility. 

Observation 18, 19, 20, 

21, 22, 32, 33, 34, 35 

Measured concentrations indicate that 

actual fluridone concentrations 

average ≥79% of nominal. 

Given that mortality started occurring 

primarily below known fluridone solubility, 

and concerns with including results above 

known solubility without actual raw 

measured fluridone concentrations, these 

treatments were dropped by the reviewer 

from calculation of toxicity endpoints. The 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

8

Test Species 

Observation Number 

Summary of Analytical Results* Are the exposure concentrations reliable?

test material concentrations below solubility 

were considered stable and nominal 

concentrations representive of exposure 

within current guideline standards. 

Observation 36 Measured concentrations were <79% 

of nominal. 

Nominal concentrations are not reliable but 

the study authors calculated the toxicity 

endpoint based on measured concentrations, 

which would be appropriate and within 

current guideline standards. 

*From pages 405-408 of study report. 

(EPA test guidance recommends that test material concentration not decline to less than 80% of the initial (day-0) measured concentration and 

the ratio of the highest concentration to the lowest concentration should be at or less than 1.5:1 among replicates of the same treatment level.)] 

 Storage conditions of test material: Not reported. 

Table 3. Physicochemical properties of fluridone. 

Parameter Values Comments 

Water solubility at 20oC 12.3 mg/L at 25 oC 

 Footprint @ 

http://sitem.herts.ac.uk/aeru/footprint/en/, accessed on 

April 28, 2009 

Vapor pressure 9.77 Η 10-8

<1 Η 10-7

USEPA 2007c 

Weed Science, 4th Ed. 2002 

UV absorption Not reported 

pKa 1.73* R. Grover and A. J. Cessna. 1991. Environmental 

Chemistry of Herbicides Volume II, CRC Press 

pKb 12.3* 

Mackay, D., Shiu, W. Y., Ma, K. C., & Lee, S. C. 

2006. Handbook of Physical-Chemical Properties 

and Environmental Fate for Organic Chemicals

(Second ed. Vol. IV). Boca Raton, FL: CRC Press. 

Kow 74.13 

Log Kow 1.87 from Footprint @ 

http://sitem.herts.ac.uk/aeru/footprint/en/, accessed on 

April 28, 2009 

Aquatic photolysis half-life 0.9-2.3 days MRID # 103326 

(EPA/OECD guidance recommends reporting water solubility under test conditions, stability in water and light, pKa, Pow, and vapor pressure of 

test material..) 

* Therefore, the unionized form should be present within the environmentally relevant pH range. 

2. Test organism: 

Species: Rainbow Trout (Oncorhynchus mykiss formerly Salmo gairdneri)

Bluegill Sunfish (Lepomis macrochirus)

Channel Catfish (Ictalurus punctatus)

Fathead Minnnow (Pimephales promelas) 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

9

(EPA test guideline preference (850.1075 and 40CFR158) is Rainbow Trout (Oncorhynchus mykiss) for a cold water species, is Bluegill Sunfish 

(Lepomis macrochirus) for a warm water species, and silverside Mednida species (Menedia menedia, M. peninsulae, or M. beryllina) for 

estuarine/marine species. Other fresh water test guideline species include Atlantic Salmon, Salmo salar; Brook Trout, Salvelinus fontinalis; 

Channel Catfish, Ictalurus punctatus; Coho Salmon, Oncorhynchus kisutch; Common Carp, Cyprinus carpio; Fathead Minnnow, Pimephales 

promelas; Guppy, Poecilia reticulata; Medaka, Oryzias latipes; and Zebra Fish, Brachydanio rerio. Another estuarine/marine test guideline 

species includes the Sheepshead Minnow, Cyprinodon variegates.)

Age at test initiation: Rainbow Trout (RBT) fingerlings

 Bluegill Sunfish (BG) fingerlings 

 Channel Catfish (CCF) fingerlings 

 Fathead Minnnow (FHM) fingerlings 

(EPA/OECD guideline age is juvenile fish and should be the same for all test organisms at test initiation)

Weight at study initiation: RBT (mean 0.7 – 1.2 g)

 BG (mean 0.5 g) 

 CCF (mean 0.4 – 0.9g) 

 FHM (mean 0.8 g) 

(EPA guideline is that juvenile fish <3.0 g should be used)

Length at study initiation: Not reported 

(EPA guidance recommends the longest should not be more than twice the length of the shortest; OECD guidance recommends length 

at study initiation of 2.0 ∀ 1.0 cm for Bluegill, Guppy, Fathead Minnow, Medaka, and Zebra Fish; 3.0 ∀ 1.0 cm for Common Carp; 

and 5.0 ∀ 1.0 cm for Rainbow Trout.)

Source: Laboratory cultures (fathead minnow and channel catfish) or federal fish hatcheries (rainbow 

trout and bluegill) 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

10

B. STUDY DESIGN:

1. Experimental Conditions 

a) Range-finding Study: None reported in study report. 

b) Definitive Study

Table 4. Experimental Parameters 

Remarks 

Parameter Details 

Guidance

Acclimation:

Period: 

Conditions: (same as test or not) 

Feeding: 

Health: (any mortality observed) 

3-4 days 

Water was gradually changed from well 

water to reconstituted test water. 

Alkalinity was reduced from 237 mg/L 

CaCO3 in well water to 35 mg/L as 

CaCO3 in the standard, temperature and 

pH test series and 3.5 mg/L in one CCF 

test (was 235 mg/L for hardness test series 

and for RBT temperature test series). 

Hardness was reduced from 272 mg/L 

CaCO3 in well water to 40* mg/L as 

CaCO3 in the standard, pH, and 

temperature test series and one series of 

hardness tests but was increased to 320 

mg/L as CaCO3 in the other. (*42 mg/L 

in the CCF pH test series). 

For RBT, temperature was reduced from 

17 o

C to 12 oC for standard, pH and 

hardness test series, and from 17 o

C to 7 o

C, 17 oC to 12 oC for temperature series. 

For CCF holding temperature was 

reduced from 17 o

C to 12 oC, and 

increased from 17 o

C to 22 oC for the 

temperature series. 

For BG, temperature was increased from 

17 o

C to 22 oC. 

Not fed during acclimation (3-4 days) 

Not reported 

EPA/OECD guidance recommends a 

minimum 12-day holding period with at least 

7 days of this period used for acclimation to 

test conditions. 

EPA/OECD test guidance is for test fish not 

to be fed 24 hours prior to test initiation. If 

fish are larger than 0.5 g EPA guidance 

recommends that this be extended to 48 

hours.

Duration of the test 96-hours 

EPA/OECD test guidance is a minimum of 96 

hours.
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

11

Remarks 

Parameter Details 

Guidance

Test condition:

Static/Static Renewal/Flowthrough 

Type of dilution system for flowthrough method 

(Flow rate for flow-through 

system or Renewal rate for static 

renewal) 

Static 

Not applicable 

Not applicable 

EPA recommends a minimum flow rate of 5 

volume additions per 24 hours. To document 

reproducibility and stability (+10%) meter 

systems should be calibrated before the study 

and checked twice daily during the test 

period.

Aeration, if any None 

EPA/OECD guidance allows aeration in test 

chambers in static systems with increased 

analyses of test material to document its 

stability, but it is not recommended in flowthrough systems. 

Loading in some test vessels 

exceeded OPPTS guideline 

recommendations but not OECD 

guideline recommendations; control 

mortality levels were acceptable 

indicating that the loading did not 

cause adverse effects on fish. 

Test vessel:

Material: 

Size: 

Fill volume:

wide mouth glass jars 

18.9 L (5 gallon) 

15 L of test solution 

Loading: ranged from 0.3 g/L to 0.8 g/L 

Meets recommended loading. EPA 

recommends loading in static/static renewal 

of <0.5 g wet weight/L and in flow-through 

systems <0.5 g wet weight/L/24 hours. 

OECD recommends a maximum of 1 g fish/L 

for static and semi-static with higher rates 

accepted for flow-through

Source of dilution water Reconstituted water made from deionized 

water of at least 106

 ohms resistivity and 

addition of reagent grade chemicals. 

Water was mixed thoroughly and aerated 

before use. 

EPA prefers soft reconstituted water or water 

from a natural source not dechlorinated tap 

water. However, use of dechlorinated tap 

water may be supportable if the biological 

responses for the organisms and chemical 

analyses of residual chlorine meet conditions 

in the Agency’s 850.1010 guidance for 

dilution water (http://www.epa.gov/opptsfrs/ 

publications/OPPTS_Harmonized/ 

850_Ecological_Effects_Test_Guidelines/ 

Drafts/850-1010.pdf)
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

12

Remarks 

Parameter Details 

Guidance

Water parameters: 

Hardness (freshwater) 

pH 

Dissolved oxygen 

Total organic carbon 

Particulate matter 

Metals 

Pesticides 

Chlorine 

Alkalinity 

Conductivity 

Chemical oxygen demand (when 

testing cationic materials) 

Temperature 

Intervals of water quality 

measurement

40, 42, 43, and 320 mg/L as CaCO3 in 19, 

3, 1, and 3 tests, respectively (see Table 1)

See Table 1 for details 

Standard and temperature test series: 7.1-

7.5 

pH test series: 6.5, 7.5, 8.5 (one 8.5 test 

with CCF varied from 7.9 – 8.65, the test 

was repeated) 

Hardness test series: 8.1 

Not reported 

Not reported, but deionized water should 

be within acceptable levels 

Not reported, but deionized water should 

be within acceptable levels 

Within acceptable levels 

<0.01 mg/L 

Not applicable 

3.5, 35, and 235 mg/L as CaCO3 in 1, 17, 

and 7 tests, respectively (see Table 1) 

< 106

 ohms resistivity 

Not applicable 

See Table 1 for specific tests 

RBT 12 o

C for all test series, and 7 and 17 o

C added for temperature series. 

BG and FHM 22 o

C 

CCF 22 o

C for all test series, and 7 and 17 o

C added for temperature series. 

Monitoring not reported but based on 

protocols followed temperatures would 

have been maintained within +1 o

C during 

the tests. 

Standard reconstituted water recipe was 

used, monitoring of DO, and pH during 

the test was not reported, except for pH in 

pH test series which is reported every 24 

hours. Hardness, pH and alkalinity would 

be at least at test initiation. 

EPA test guidance consists of: 

hardness: <250 mg/l as CaCO3 (40 – 48 mg/l 

CaCO3 for metals) (OECD guidance is 10 – 

250 mg/l CaCO3) 

pH: 6.0 – 8.5 for freshwater fish with 

preferably 7.2-7.6; 7.5-8.5 for saltwater fish 

with preferably 8.0-8.3 for marinestenohaline fishes; 7.7-8.0 for estuarineeuryhaline fishes (OECD guidance is 6-8.5) 

Temperature: 22 oC is preferred for the 

warm water fish: Bluegill, Channel Catfish, 

Common Carp, Guppy, Medaka, Sheepshead 

Minnow and the silversides; 12 oC is 

preferred for the cold water fish: Atlantic 

Salmon, Brook Trout, Coho Salmon, Rainbow 

Trout, and Zebra Fish (OECD temperature 

guidance is 13 – 17 oC for Rainbow Trout, 

20 – 24 oC for Common Carp, and 21 – 25 

for Bluegill, Fathead Minnow, Guppy, 

Medaka, and Zebra Fish.) 

During a given test the selected temperature 

should be constant within ± 1 oC (OECD 

recommends constant within + 2 o

C) 

Dissolved oxygen: 60 - 100% saturation 

(OECD guidance is >60% saturation) 

Total organic carbon: guidance is < 2 mg/L 

(OECD guidance is also < 2 mg/L) 

Salinity: recommended is 30-34 parts per 

thousand; weekly range < 6 %) 

Treatment concentrations: Nominal Concentrations: 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

13

Remarks 

Parameter Details 

Guidance

Nominal, corrected for purity: 

Measured: 

See Tables 6, 7, 8, and 9 for specific tests. 

All tests included 3.2, 5.6, 10, 18, and 32 

mg a.i./L nominal 

(RBT tests also included 1.8 and 56; 1.0; 

and 100 mg a.i./L in 9, 8, and 1 test(s), 

respectively, out of the 10 RBT tests) 

(FHM tests also included 0.56 and 1.0; 

56; and 100 mg a.i./L in 1, 2, and 1 test(s), 

respectively, out of the 2 FHM tests)—

same holds for the 2 BG tests 

(CCF tests also included 0.56; 1.0; 1.8; 

56; 100; and 4.2, 7.5, 13.5, and 24 in 6, 7, 

8, 2, 1 and 1 test(s), respectively, out of 

the 11 CCF tests) 

TGAI tests included both a solvent 

(acetone) and a negative control 

EP tests did not use an additional solvent 

for stock solutions or direct additions, 

which is appropriate, so only negative 

controls were tested 

See Tables 1, 2, and 6 for details 

EPA/OECD guidance for a definitive test is at 

least 5 treatment levels in a geometric series 

which bracket the desired LC50 plus a 

dilution control and a solvent control, if a 

solvent was used. EPA prefers the spacing 

factor be at least 1.6 and OECD prefers the 

spacing factor not exceed 2.2.

Solvent (type and concentration, 

if used) 

TGAI tests: Acetone 0.5 mL/L for fish 

Additional solvent not used for EP tests 

EPA/OECD recommends that solvents should 

not exceed 0.1 ml/l

Number of replicates:

Control (dilution water): 

Solvent control: 

Treatments: 

None EPA/OECD guidance is for no replicates but 

EPA prefers a minimum of 2. 

Number of organisms per 

replicate: 

Control (dilution water): 

Solvent control: 

Treatments: 

10 

10 

10 

EPA/OECD guidance recommends a 

minimum number of 7 organisms per 

replicate. EPA prefers a minimum of 10 fish 

per replicate. 

Lighting 16 hours light:8 hours dark 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

14

Remarks 

Parameter Details 

Guidance

Laboratory lighting using a combination 

of Duro-test and wide-spectrum Growlux 

bulbs were reported for chronic testing at 

the lab and may have been used for acute 

testing. Lighting parameters were not 

reported; however, the lighting set up 

should be consistent with the ASTM 1980 

testing parameters and the Committee on 

Methods for Toxicity Tests with Aquatic 

Organisms (1975). 

EPA/OECD recommended photoperiod 

regime is selected from 12 – 16 hours of light 

with the selected regime constant during test.

Feeding Not fed 

EPA/OECD test guidance is for test 

organisms not to be fed during the study.

Recovery of test material: 

Method recoveries and frequency 

of determination 

% of Nominal 

Level of Quantitation

Level of Detection 

A coefficient of variation of 1.8 (N=8) 

and 3.5% (N=8) was obtained for control 

pond water fortified with 1.0 ppm and 

0.05 ppm of fluridone, respectively 

See Table 2. 

The method was reportedly able to detect 

~0.005 – 0.001 mg fluridone/L 

Positive control (if used, indicate 

the reference test material and 

concentrations} 

Not applicable 

Other parameters, if any 

2. Observations:

Table 5. Observations 

Remarks

Parameter Details 

Guidance 

Parameters measured 

including the sublethal 

effects/toxicity symptoms

Observed for survival, loss of 

equilibrium, immobility, 

surfacing, labored respiraton, 

rapid respiration, pigmentation 

changes 

Observation intervals 24-hours 

EPA/OECD recommends that observation intervals 

be a minimum of every 24 hours. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

15

Remarks

Parameter Details 

Guidance 

Were raw data included? Mortality and sublethal effects 

raw data was included in the 

Federal Lab data sheets but 

analytical test material 

concentration raw data were not 

included. 

Other observations, if any 

II. RESULTS and DISCUSSION:

A. MORTALITY:

No mortality/immobility was observed in any of the controls. No replicates were available to determine 

NOAEC values statistically. Mortality was observed in all tests at some treatment level. Many treatment 

levels were greater than solubility and were not used in statistical analysis because the exposure 

concentration is unknown. This results in some tests not having enough treatment levels to statistically 

analyze the results. (EPA/OECD test guidance is < 10% control mortality.) 

Table 6. Effect of fluridone on mortality of freshwater fish Rainbow Trout. (*Treatment levels are above 

solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone average concentration >79% 

nominal. Treatments in bold were used by reviewer to calculate toxicity endpoint.) 

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr Treatment 

(mg a.i./L) 

Nominal 

pH 

Hardness 

as mg/L 

CaCO3

Temp 

o

C 

No. of 

Fish at 

Test 

Initiation

No. 

Dead

% 

Mort 

No. 

Dead

% 

Mort 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

Obs 13 7.3 40 12 Observation 13, TGAI, Reconstituted Water 

32* 10 7 70 8 80 9 90 10 100 

18* 10 3 30 7 70 9 90 10 100 

10 (6.4**) 10 2 20 5 50 10 100 10 100 

5.6 (4.1 **) 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 14 7.1 40 12 Observation 14, EP, Reconstituted Water 

100* 10 10 100 10 100 10 100 10 100 

56* 10 8 80 10 100 10 100 10 100 

32* 10 3 30 9 90 10 100 10 100 

18* 10 0 0 8 80 10 100 10 100 

10 (6.3**) 10 0 0 1 10 8 80 10 100 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

16

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr Treatment 

(mg a.i./L) 

Nominal 

pH 

Hardness 

as mg/L 

CaCO3

Temp 

o

C 

No. of 

Fish at 

Test 

Initiation

No. 

Dead

% 

Mort 

No. 

Dead

% 

Mort 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

5.6 10 0 0 0 0 0 0 3 30 

3.2 (2.9**) 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 15 7.5 40 7 Observation 15, TGAI, Reconstituted Water 

56* 10 10 100 10 100 10 100 10 100 

32* 10 10 100 10 100 10 100 10 100 

18* 10 3 30 8 80 9 90 10 100 

10 10 1 10 7 70 7 70 9 90 

5.6 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

1.0 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 16 7.5 40 12 Observation 16, TGAI, Reconstituted Water 

56* 10 10 100 10 100 10 100 10 100 

32* 10 10 100 10 100 10 100 10 100 

18* 10 10 100 10 100 10 100 10 100 

10 10 5 50 8 80 9 90 10 100 

5.6 10 4 40 4 40 5 50 9 90 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

1.0 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 17 7.5 40 17 Observation 17, TGAI, Reconstituted Water 

56* 10 10 100 10 100 10 100 10 100 

32* 10 10 100 10 100 10 100 10 100 

18* 10 10 100 10 100 10 100 10 100 

10 10 10 100 10 100 10 100 10 100 

5.6 10 2 20 6 60 8 80 10 100 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

1.0 10 0 0 0 0 0 0 0 0 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

17

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr Treatment 

(mg a.i./L) 

Nominal 

pH 

Hardness 

as mg/L 

CaCO3

Temp 

o

C 

No. of 

Fish at 

Test 

Initiation

No. 

Dead

% 

Mort 

No. 

Dead

% 

Mort 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 18 6.5 40 12 Observation 18, TGAI, Reconstituted Water 

56*~ 10 10 100 10 100 10 100 10 100 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 2 20 7 70 10 100 10 100 

10~ 10 4 40 7 70 9 90 9 90 

5.6~ 10 0 0 2 20 4 40 6 60 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 19 7.5 40 12 Observation 19, TGAI, Reconstituted Water 

56*~ 10 10 100 10 100 10 100 10 100 

32*~ 10 7 70 8 80 8 80 9 90 

18*~ 10 3 30 7 70 9 90 9 90 

10~ 10 0 0 9 90 10 100 10 100 

5.6~ 10 0 0 3 30 4 40 5 50 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 20 8.5 40 12 Observation 20, TGAI, Reconstituted Water 

56*~ 10 4 40 10 100 10 100 10 100 

32*~ 10 8 80 10 100 10 100 10 100 

18*~ 10 3 30 9 90 10 100 10 100 

10~ 10 0 0 6 60 9 90 10 100 

5.6~ 10 0 0 0 0 0 0 1 10 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

18

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr Treatment 

(mg a.i./L) 

Nominal 

pH 

Hardness 

as mg/L 

CaCO3

Temp 

o

C 

No. of 

Fish at 

Test 

Initiation

No. 

Dead

% 

Mort 

No. 

Dead

% 

Mort 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

Obs 21 8.1 40 12 Observation 21, TGAI, Reconstituted Water 

56*~ 10 10 100 10 100 10 100 10 100 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 0 0 0 0 8 80 9 90 

10~ 10 0 0 4 40 6 60 9 90 

5.6~ 10 0 0 0 0 2 20 3 30 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs 22 8.1 320 12 Observation 22, TGAI, Reconstituted Water 

56*~ 10 10 100 10 100 10 100 10 100 

32*~ 10 8 80 9 90 10 100 10 100 

18*~ 10 2 20 4 40 8 80 10 100 

10~ 10 0 0 1 10 8 80 9 90 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

19

Table 7. Effect of fluridone on mortality of freshwater fish Fathead Minnow. (*Treatment levels are above 

solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone average concentration >79% 

nominal. Treatments in bold were used by reviewer to calculate toxicity endpoint.)

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr 

Treatmen

t 

(mg a.i./L) 

Nominal 

pH Hardness

, CaCO3 

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dead 

% 

Mort 

No. 

Dead

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dead

% 

Mort 

Obs. 24 7.1 40 22 Observation 24, EP, Reconstituted Water 

100* 10 10 100 10 100 10 100 10 100 

56* 

(10.2**) 

 10 10 100 10 100 10 100 10 100 

32* 10 0 0 1 10 1 10 2 20 

18* 

(6.7**) 

 10 0 0 0 0 0 0 0 0 

10 10 0 0 0 0 0 0 0 0 

5.6 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 23 7.1 40 22 Observation 23,TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 0 0 0 0 0 0 1 10 

10~ 10 0 0 0 0 0 0 0 0 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs.=observation 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

20

Table 8. Effect of fluridone on mortality of freshwater fish Channel Catfish. (*Treatment levels are above 

solubility (12.3 mg/L). ~Analytical fluridone average concentration >79% nominal. Treatments in bold were used 

by reviewer to calculate toxicity endpoint. pt = precipitate observed)

Cummulative Mortality at Observation Period 

Treatment 24-hr 48-hr 72-hr 96-hr 

(mg a.i./L) 

Nominal 

pH Hardness, 

CaCO3 

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dea

d 

% 

Mort 

Obs. 25 7.1 40 22 Observation 25, TGAI, Reconstituted Water 

56*, pt 10 10 100 10 100 10 100 10 100 

32*, pt 10 10 100 10 100 10 100 10 100 

18*, pt 10 10 100 10 100 10 100 10 100 

10 10 0 0 0 0 0 0 0 0 

5.6 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

1.0 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 26 7.1 40 22 Observation 26, EP, Reconstituted Water 

100* 10 10 100 10 100 10 100 10 100 

56* 10 10 100 10 100 10 100 10 100 

32* 10 4 40 10 100 10 100 10 100 

18* 10 0 0 10 100 10 100 10 100 

10 10 0 0 0 0 0 0 0 0 

5.6 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 27 7.1 40 12 Observation 27, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 9 90 10 100 10 100 10 100 

10~ 10 0 0 0 0 2 20 5 50 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 28 7.1 40 17 Observation 28, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

21

Cummulative Mortality at Observation Period 

Treatment 24-hr 48-hr 72-hr 96-hr 

(mg a.i./L) 

Nominal 

pH Hardness, 

CaCO3 

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dea

d 

% 

Mort 

18*~ 10 10 100 10 100 10 100 10 100 

10~ 10 0 0 0 0 1 10 1 10 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 29 7.1 40 22 Observation 29, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 2 20 9 90 10 100 10 100 

10~ 10 0 0 0 0 0 0 0 0 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 30 6.5 42 22 Observation 30, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 0 0 4 40 10 100 10 100 

10~ 10 0 0 0 0 0 0 1 10 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 31 7.5 42 22 Observation 31, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 0 0 10 100 10 100 10 100 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

22

Cummulative Mortality at Observation Period 

Treatment 24-hr 48-hr 72-hr 96-hr 

(mg a.i./L) 

Nominal 

pH Hardness, 

CaCO3 

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dea

d 

% 

Mort 

10~ 10 0 0 0 0 0 0 0 0 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 32 8.5 42 22 Observation 32, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 1 10 1 10 5 50 10 100 

10~ 10 0 0 2 20 6 60 9 90 

5.6~ 10 0 0 0 0 0 0 1 10 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 0 0 0 0 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 33 8.1 40 22 Observation 33, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 7 70 8 80 10 100 10 100 

10~ 10 0 0 0 0 0 0 6 60 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 34 8.1 320 22 Observation 34, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 6 60 10 100 10 100 10 100 

10~ 10 0 0 0 0 0 0 0 0 

5.6~ 10 0 0 0 0 0 0 0 0 

3.2~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

23

Cummulative Mortality at Observation Period 

Treatment 24-hr 48-hr 72-hr 96-hr 

(mg a.i./L) 

Nominal 

pH Hardness, 

CaCO3 

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dea

d 

% 

Mort 

Control (Dilution Water 

Only) 10 0 0 0 0 0 0 0 0 

7/17/80 8.5 430 22 Observation 7/17/80, TGAI, Reconstituted Water 

24* 10 10 100 10 100 10 100 10 100 

18* 10 10 100 10 100 10 100 10 100 

13.5* 10 3 30 10 100 10 100 10 100 

10 10 0 0 2 20 7 70 10 100 

7.5 10 0 0 0 0 0 0 0 0 

5.6 10 0 0 0 0 0 0 0 0 

4.2 10 0 0 0 0 0 0 0 0 

3.2 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. = observation 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

24

Table 9. Effect of fluridone on mortality of freshwater fish Bluegill Sunfish. (*Treatment levels are above 

solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone average concentration >79% 

nominal. Treatments in bold were used by reviewer to calculate toxicity endpoint.)

Cumulative Mortality at Observation Period 

24-hr 48-hr 72-hr 96-hr Treatmen

t 

(mg a.i./L) 

Nominal 

pH Hardness 

as CaCO3

Temp 

o

C 

No. of 

Fish at 

Test 

Initiatio

n 

No. 

Dea

d 

% 

Mort 

No. 

Dead 

% 

Mort

No. 

Dead 

% 

Mort 

No. 

Dead 

% 

Mort 

Obs. 36 7.1 40 22 Observation 36, EP, Reconstituted Water 

100* 10 10 100 10 100 10 100 10 100 

56* 10 10 100 10 100 10 100 10 100 

32* 10 10 100 10 100 10 100 10 100 

18* 10 5 50 9 90 9 90 9 90 

10 10 0 0 4 40 4 40 4 40 

5.6 10 0 0 0 0 0 0 1 10 

3.2 10 0 0 0 0 0 0 0 0 

1.8 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs. 35 7.1 40 22 Observation 35, TGAI, Reconstituted Water 

32*~ 10 10 100 10 100 10 100 10 100 

18*~ 10 10 100 10 100 10 100 10 100 

10~ 10 2 20 2 20 2 20 2 20 

5.6~ 10 1 10 1 10 1 10 1 10 

3.2~ 10 0 0 0 0 0 0 0 0 

1.8~ 10 0 0 0 0 1 10 1 10 

1.0~ 10 0 0 0 0 0 0 0 0 

0.56~ 10 0 0 0 0 0 0 0 0 

Solvent Control (acetone) 10 0 0 0 0 0 0 0 0 

Negative Control 10 0 0 0 0 0 0 0 0 

Obs.=observation 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data. 

B. NON-LETHAL TOXICITY ENDPOINTS:

Immobility, loss of equilibrium, and surfacing were all observed for rainbow trout, channel catfish, 

bluegill, and fathead minnow. Table 10 summarizes the lowest concentrations where sublethal effects 

were observed in all tests for each species and Table 11 summarizes the sublethal effects observed in all 

studies. Rainbow trout were the most sensitive and fathead minnow and bluegill were the least sensitive. 

Immobility and loss of equilibrium were observed at 3.2 mg a.i./L and surfacing was observed at 5.6 mg 

a.i./L. No statistical analysis was performed. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

25

Table 10. Summary of the lowest concentrations that sublethal effects were observed and the highest 

concentration in the corresponding test where the sublethal effect was not observed. Statistics could not be 

completed because no replicates were available. 

Study Parameter Immobility Loss of Equilibrium Surfacing 

Rainbow Trout, TGAI 

LOAEC 3.2 5.6 5.6 

NOAEC 1.8 3.2 3.2 

Observation 15 and others 13 17 

Rainbow Trout, EP 

LOAEC 5.6 3.2 0 

NOAEC 3.2 1.8 0 

Observation 14 14 14 

Channel Catfish, TGAI 

LOAEC 5.6 10 7.5 

NOAEC 3.2 5.6 5.6 

Observation 27 and others 33 A 

Bluegill, EP 

LOAEC 18 18 NA 

NOAEC 10 10 

Observation 36 36 

Bluegill, TGAI 

LOAEC 5.6 

NOAEC 3.2 

Observation NA NA 35 

Fathead Minnow, TGAI 

LOAEC 18 18 

NOAEC 10 10 

Observation 23 23 NA 

Fathead Minnow, EP 

LOAEC 32 

NOAEC 18 

Observation 24 NA NA 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

26

Table 11. Sublethal effects(a) of fluridone on freshwater Rainbow trout. (All controls had no sublethal effects. 

*Treatment levels are above solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone 

average concentration >79% nominal.)

Effects at Observation period 

% Immobilized % Loss of equilibrium (LOE) 

(# immobilized/# live) (# LOE/# live) 

Treatment 

(mg a.i./L) 

Nominal 

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

Observation 13, Rainbow Trout, TGAI, pH 7.3, 40 mg/L as Ca CO3, 12 oC 

32* 100 (3/3) 100 (2/2) 100 (1/1) All dead 0 (0/3) 0 (0/2) 0 (0/1) All dead 

18* 100 (7/7) 100 (3/3) 100 (1/1) All dead 0 (0/7) 0 (0/3) 0 (0/1) All dead 

10 (6.4**) 100 (8/8) 100 (5/5) All dead All dead 0 (0/8) 0 (0/5) All dead All dead 

5.6 (4.1**) 70 (7/10) 80 (8/10) 80 (8/10) 80 (8/10) 10 (1/10) 20 (2/10) 20 (2/10) 0 (0/10) 

3.2 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

Observation 14, Rainbow Trout, EP, pH 7.1, 40 mg/L as CaCO3, 12 oC 

100* All dead All dead All dead All dead All dead All dead All dead All dead 

56* 100 (2/2) All dead All dead All dead 0 (0/2) All dead All dead All dead 

32* 100 (7/7) 100 (1/1) All dead All dead 0 (0/7) 0 (0/1) All dead All dead 

18* 100 

(10/10) 100 (2/2) All dead All dead 0 (0/10) 0 (0/2) All dead All dead 

10 (6.3**) 100 

(10/10) 100 (9/9) 100 (2/2) All dead 0 (0/10) 0 (0/9) 0 (0/2) All dead 

5.6 40 (4/10) ? (b) 70 (7/10) 71 (5/7) 0 0 0 (0/10) 0 (0/7) 

3.2 (2.9**) 0 0 0 0 (0/10) 0 0 0 10 (1/10)

1.8 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

Observation 15, Rainbow Trout, TGAI, pH 7.5, 40 mg/L as CaCO3, 7 oC 

56* All dead All dead All dead All dead 

32* All dead All dead All dead All dead 

18* 100 (7/7) 100 (2/2) 100 (1/1) All dead 

10 100 (9/9) 100 (3/3) 100 (3/3) 100 (1/1)

5.6 60 (6/10 80 (8/10) 60 (6/10) 60 (6/10)

3.2 0 0 0 10 (1/10)

1.8 0 0 0 0 (0/10) 

1.0 0 0 0 0 (0/10) 

None observed 

Observation 16, Rainbow Trout, TGAI, pH 7.5, 40 mg/L as CaCO3, 12 oC 

56* All dead All dead All dead All dead 

32* All dead All dead All dead All dead 

18* All dead All dead All dead All dead 

10 100 (5/5) 100 (2/2) 100 (1/1) All dead 

5.6 100 (6/6) 83 (5/6) 80 (4/5) 100 (1/1)

None observed 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

27

Effects at Observation period 

% Immobilized % Loss of equilibrium (LOE) 

(# immobilized/# live) (# LOE/# live) 

Treatment 

(mg a.i./L) 

Nominal 

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

3.2 10 (1/10) 0 0 0 (0/10) 

1.8 0 0 0 0 (0/10) 

1.0 0 0 0 0 (0/10) 

Observation 17, Rainbow Trout, TGAI, pH 7.5, 40 mg/L as CaCO3, 17 oC 

56* All dead All dead All dead All dead All dead All dead All dead All dead 

32* All dead All dead All dead All dead All dead All dead All dead All dead 

18* All dead All dead All dead All dead All dead All dead All dead All dead 

10 All dead All dead All dead All dead All dead All dead All dead All dead 

5.6 88 (7/8) 

(Surf 1/8) 75 (3/4) 100 (2/2) All dead 0 (0/8) 25 (1/4) 0 (0/2) All dead 

3.2 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

1.8 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

1.0 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

Observation 18, Rainbow Trout, TGAI, pH 6.5, 40 mg/L as CaCO3, 12 oC 

56*~ All dead All dead All dead All dead 

32*~ All dead All dead All dead All dead 

18*~ 100 (8/8) 100 (3/3) All dead All dead 

10~ 100 (6/ 6) 100 (3/3) 100 (1/1) 100 (1/1)

5.6~ 70 (7/10) 88 (7/8) 100 (6/ 6) 100 (4 /4)

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

None observed 

Observation 19, Rainbow Trout, TGAI, pH 7.5, 40 mg/L as CaCO3, 12 oC 

56*~ All dead All dead All dead All dead 

32*~ 100 (3/3) 100 (2/2) 100 (2/2) 100 (1/1)

18*~ 100 (7/7) 100 (3/3) 100 (1/1) 100 (1/1)

10~ 100 

(10/10) 100 (1/1) All dead All dead 

5.6~ 100 

(10/10) 100 (7/7) 100 (6/6) 80 (4/5) 

3.2~ 0 10 (1/10) 0 (0/10) 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

None observed 

Observation 20, Rainbow Trout, TGAI, pH 8.5, 40 mg/L as CaCO3, 12 oC 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

28

Effects at Observation period 

% Immobilized % Loss of equilibrium (LOE) 

(# immobilized/# live) (# LOE/# live) 

Treatment 

(mg a.i./L) 

Nominal 

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

56*~ 100 (6/6) All dead All dead All dead 

32*~ 100 (2/2) All dead All dead All dead 

18*~ 100 (7/7) 100 (1/1) All dead All dead 

10~ 100 

(10/10) 100 (4/4) 100 (1/1) All dead 

5.6~ 70 (7/10) 60 (6/10) 70 (7/10) 67 (6/9) 

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

None observed 

Observation 21, Rainbow Trout, TGAI, pH 8.1, 40 mg/L as CaCO3, 12 oC 

56*~ All dead All dead All dead All dead 

32*~ All dead All dead All dead All dead 

18*~ 100 

(10/10) 

100 

(10/10) 100 (2/2) 100 (1/1)

10~ 100 

(10/10) 100 (6/6) 100 (4/4) 100 (1/1)

5.6~ 100 

(10/10) 80 (8/10) 88 (7/8) 100 (7/7)

3.2~ 10 (1/10) 0 0 0 (1/10) 

1.8~ 0 0 0 0 (1/10) 

1.0~ 0 0 0 0 (1/10) 

None observed 

Observation 22, Rainbow Trout, TGAI, pH 8.1, 320 mg/L as CaCO3, 12 oC 

56*~ All dead All dead All dead All dead 

32*~ 100 (2/2) 100 (1/1) All dead All dead 

18*~ 100 (8/8) 100 (6/6) 100 (2/2) All dead 

10~ 100 

(10/10) 100 (9/9) 100 (2/2) 0 (0/1) 

5.6~ 80 (8/10) 80 (8/10) 90 (9/10) 90 (9/10)

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (1/10) 

1.0~ 0 0 0 0 (1/10) 

None observed 

(a) The only sublethal effects observed consistently were immobilization and loss of equilibrium. There was one incident of 

surfacing in one fish, test 17 at the 5.6 mg/L nominal treatment at 24 hours. 

(b) ? = value on raw data sheet was illegible. 

Surf = surfacing 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data.
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

29

Table 12. Sublethal effects of fluridone on freshwater Fathead Minnow. (All controls had no sublethal effects. 

*Treatment levels are above solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone 

average concentration >79% nominal.)

Effects at Observation period 

Immobility Loss of equilibrium 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

Observation 23, Fathead Minnow, TGAI, pH 7.1, 40 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead All dead All dead All dead All dead 

18*~ 90 (9/10) 90 (9/10) 90 (9/10) 0 (0/9) 0 10 (1/10) 10 (1/10) 0 (0/9) 

10*~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

5.6~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

3.2~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

0.56~ 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

Observation 24, Fathead Minnow, EP, pH 7.1, 40 mg/L as CaCO3, 22 oC 

100* All dead All dead All dead All dead 

56* (10.2**) All dead All dead All dead All dead 

32* 90 (9/10) 100 (9/9) 100 (9/9) 0 (0/8) 

18* (6.7**) 0 0 0 0 (0/10) 

10 0 0 0 0 (0/10) 

5.6 0 0 0 0 (0/10) 

3.2 0 0 0 0 (0/10) 

1.8 0 0 0 0 (0/10) 

None observed 

(a) The only sublethal effects observed were immobilization and loss of equilibrium. 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data.
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

30

Table 13. Sublethal effects(a) of fluridone on freshwater Channel Catfish. (*Treatment levels are above 

solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone average concentration >79% 

nominal.)

Effects at Observation period 

Immobility Surfacing 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

Observation 25, Channel Catfish, TGAI, pH 7.1, 40 mg/L as CaCO3, 22 oC 

56*, pt All dead All dead All dead All dead 

32*, pt All dead All dead All dead All dead 

18*, pt All dead All dead All dead All dead 

10 80 (8/10) 60 (6/10) 40 (4/10) 0 (0/10) 

5.6 0 0 0 0 (0/10) 

3.2 0 0 0 0 (0/10) 

1.8 0 0 0 0 (0/10) 

1.0 0 0 0 0 (0/10) 

None observed 

(Loss of equilibrium observed at 10 ppm 

nominal in 2, 4, and 5 fish out of 10 at 48, 72, 

and 96 hours, respectively) 

Observation 26, Channel Catfish, EP, pH 7.1, 40 mg/L as CaCO3, 22 oC 

100* All dead All dead All dead All dead 

56* All dead All dead All dead All dead 

32* 100 

6 of 6 All dead All dead All dead 

18* 100 

10 of 10 All dead All dead All dead 

10 0 0 0 0 

5.6 0 0 0 0 

3.2 0 0 0 0 

1.8 0 0 0 0 

None observed 

Observation 27, Channel Catfish, TGAI, pH 7.1, 40 mg/L as CaCO3, 12 oC 

32*~ All dead All dead All dead All dead All dead All dead All dead All dead

18*~ 100 (1/1) All dead All dead All dead 0 (0/1) All dead All dead All dead

10~ 90 (9/10) 90 (9/10) 88 (7/8) 80 (4/5) 10 (1/10) 10 (1/10) 13 (1/8) 20 (1/5)

5.6~ 20 (2/10) 20 (2/10) 60 (6/10) 100 

(10/10) 0 0 0 0 (0/10)

3.2~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.8~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.0~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

0.56~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

31

Effects at Observation period 

Immobility Surfacing 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

Observation 28, Channel Catfish, TGAI, pH 7.1, 40 mg/L as CaCO3, 17 oC 

32*~ All dead All dead All dead All dead All dead All dead All dead All dead

18*~ All dead All dead All dead All dead All dead All dead All dead All dead

10~ 90 (9/10) 100 

(10/10) 100 (9/9) 89 (8/9) 10 (1/10) 0 (0/10) 0 (0/9) 11 (1/9)

5.6~ 0 0 100 

(10/10) 

100 

(10/10) 0 0 0 0 (0/10)

3.2~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.8~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.0~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

0.56~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

Observation 29, Channel Catfish, TGAI, pH 7.1, 40 mg/L as CaCO3, 22 oC 

32~ All dead All dead All dead All dead All dead All dead All dead All dead

18*~ 100 (8/8) 100 (1/1) All dead All dead 0 (0/8) 0 (0/1) All dead All dead

10~ 70 (7/10) 100 

(10/10) 

100 

(10/10) 

100 

(10/10) 0 0 0 0 (0/10)

5.6~ 40 (4/10) 80 (8/10) 90 (9/10) 0 (0/10) 0 0 0 80 (8/10)

3.2~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.8~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

1.0~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

0.56~ 0 0 0 0 (0/10) 0 0 0 0 (0/10)

Observation 30, Channel Catfish, TGAI, pH 6.5, 42 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead 

18*~ 100 

(10/10) 100 (6/6) All dead All dead 

10~ 0 90 (9/10) 90 (9/10) 0 (0/9) 

5.6~ 0 0 0 0 (0/10) 

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

0.56~ 0 0 0 0 (0/10) 

None observed 

Observation 31, Channel Catfish, TGAI, pH 7.5, 42 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead 

18*~ 100 

10 of 10 All dead All dead All dead 

None observed 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

32

Effects at Observation period 

Immobility Surfacing 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

10~ 60 

6 of 10 

60 

6 of 10 

70 

7 of 10 0 (0/10) 

5.6~ 0 0 0 0 (0/10) 

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

0.56~ 0 0 0 0 (0/10) 

Observation 32, Channel Catfish, TGAI, pH 8.5, 42 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead 

18*~ 100 (9/9) 100 (9/9) 100 (5/5) All dead 

10~ 100 

(10/10) 100 (8/8) 25 (1/4) 0 (0/1) 

5.6~ 0 20 (2/10) 30 (3/10) 0 (0/9) 

3.2~ 0 0 0 0 (0/10) 

1.8~ 0 0 0 0 (0/10) 

1.0~ 0 0 0 0 (0/10) 

0.56~ 0 0 0 0 (0/10) 

None observed 

Observation 33, Channel Catfish, TGAI, pH 8.1, 40 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead 

18*~ 100 (3/3) 100 (2/2) All dead All dead 

10~ 90 (9/10) 50 (5/10) 50 (5/10) 0 (0/4) 

5.6~ 70 (7/10) 70 (7/10) 70 (7/10) 0 (0/10) 

3.2~ 0 0 0 0 (0/10) 

None observed 

(LOE observed at 10 ppm nominal in 5 out of 

10 fish at 48 and 72 hours) 

Observation 34, Channel Catfish, TGAI, pH 8.1, 320 mg/L as CaCO3, 22 oC 

32*~ All dead All dead All dead All dead 

18*~ 100 (4/4) All dead All dead All dead 

10~ 80 (8/10) 10 (1/10) 10 (1/10) 0 (0/10) 

5.6~ 10 (1/10) 10 (1/10) 10 (1/10) 0 (0/10) 

3.2~ 0 0 0 0 (0/10) 

None observed 

7/17/80, Channel Catfish, TGAI, pH 8.5, 43 mg/L as CaCO3, 22 oC 

24* All dead All dead All dead All dead All dead All dead All dead All dead

18* All dead All dead All dead All dead All dead All dead All dead All dead

13.5* 100 (7/7) All dead All dead All dead 0 (0/7) All dead All dead All dead
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

33

Effects at Observation period 

Immobility Surfacing 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

10 30 (3/10) 12 (1/8) 0 (0/3) 

(LOE 1/3) All dead 0 (0/10) 50 (4/8) 100 (3/3) All dead

7.5 0 0 0 0 (0/10) 0 0 10 (1/10) 0 (0/10)

5.6 0 0 0 0 (0/10) 0 0 0 0 (0/10)

4.2 0 0 0 0 (0/10) 0 0 0 0 (0/10)

3.2 0 0 0 0 (0/10) 0 0 0 0 (0/10)

(a) Sublethal effects observed consistented primarily of immobilization and surfacing. Loss of equilibrium was observed in three 

incidents: in observation # 25 at 10 ppm nominal in 2, 4, and 5 fish out of 10 at 48, 72, and 96 hours, respectively; in observation 

# 33 at 10 ppm nominal in 5 out of 10 fish at 48 and 72 hours; and in the 7/17/80 test at 10 ppm nominal. 

LOE = loss of equilibrium 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data.

Table 14. Sublethal effects(a) of fluridone on freshwater Bluegill Sunfish. (*Treatment levels are above 

solubility (12.3 mg/L). **Measured fluridone concentrations. ~Analytical fluridone average concentration >79% 

nominal.)

Effects at Observation period 

Immobility Loss of equilibrium 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

Observation 36, Bluegill, EP, pH 8.5, 43 mg/L as CaCO3, 22 oC 

100 All dead All dead All dead All dead All dead All dead All dead All dead 

56 All dead All dead All dead All dead All dead All dead All dead All dead 

32 All dead All dead All dead All dead All dead All dead All dead All dead 

18 0 (0/5) 0 (0/1) 0 (0/1) 0 (0/1) 25 (1/5) 0 (0/1) 0 (0/1) 0 (0/1) 

10 10 (1/10) 0 (0/6) 0 (0/6) 0 (0/6) 0 (0/5) 0 (0/6) 0 (0/6) 0 (0/6) 

5.6 0 0 0 (0/10) 0 (0/9) 0 0 0 (0/10) 0 (0/9) 

3.2 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

1.0 0 0 0 0 (0/10) 0 0 0 0 (0/10) 

Observation 35, Bluegill, TGAI, pH 7.1, 40 mg/L as CaCO3, 22 oC 

32 All dead All dead All dead All dead

18 All dead All dead All dead All dead

10 0 (0/8) 0 (0/8) 100 (8/8) 0 (0/8) 

5.6 0 (0/9) 0 (0/9) 100 (9/9) 0 (0/9) 

3.2 0 0 0 0 (0/10)

1.8 

None observed 

0 0 (0/10) 0 (0/9) 0 (0/9) 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

34

Effects at Observation period 

Immobility Loss of equilibrium 

(% Affected; ) (% Affected) 

Treatment 

(mg a.i./L) 

Nominal

24-hr 48-hr 72-hr 96-hr 24-hr 48-hr 72-hr 96-hr 

1.0 0 0 0 0 (0/10)

0.56 0 0 0 0 (0/10)

(a) The only sublethal effects observed were immobilization and loss of equilibrium. 

Footnotes: Data were obtained from Federal Lab Data submitted to support the Mayer and Ellersieck (1986) data.

C. REPORTED STATISTICS: 

The method used to determine the LC50 values, confidence limits, and slopes were the Litchfield and 

Wilcoxon (1949) method. 

Table 15. Statistics Reported in Study Report. 

Obs Organism Compound Temp o

C 

pH Hardness, 

mg/L as 

CaCO3

96-hr LC50 (mg 

a.i./L) with 95% 

Confidence 

interval 

Slope LC50

Method 

Standard Test 

13 Rainbow Trout TGAI 12 7.1-7.3 40 7.6 (6.0-9.7) 1.31 Litchfield* 

14 Rainbow Trout EP 12 7.1-7.3 40 7.1 (5.6-9.1) 1.48 Litchfield* 

Varied Temperature 

15 Rainbow Trout TGAI 7 7.1-7.5 40 7.7 (5.8-10.2) 1.56 Litchfield* 

16 Rainbow Trout TGAI 12 7.1-7.5 40 4.5 (3.4-5.9) 1.55 Litchfield* 

17 Rainbow Trout TGAI 17 7.1-7.5 40 4.2 (3.4-5.4) 1.31 Litchfield* 

Varied pH 

18 Rainbow Trout TGAI 12 6.5 40-42 5.7 (4.1-7.9) 1.45 Litchfield* 

19 Rainbow Trout TGAI 12 7.5 40-42 5.6 (4.8-6.6) 1.20 Litchfield* 

20 Rainbow Trout TGAI 12 8.5 40-42 8.4 (6.4-11.1) 1.37 Litchfield* 

Varied Hardness 

21 Rainbow Trout TGAI 12 8.1 40 7.6 (5.7-10.1) 1.58 Litchfield* 

22 Rainbow Trout TGAI 12 8.1 320 7.6 (5.9-9.7) 1.32 Litchfield* 

Standard Tests 

23 Fathead Minnow TGAI 22 7.1-7.3 40 22 (17-28) 1.34 Litchfield* 

24 Fathead Minnow EP 22 7.1-7.3 40 41(32-52) 1.32 Litchfield* 

Standard Test 

25 Channel Catfish TGAI 22 7.1-7.3 40 13.2 (10.3-17.0) 1.33 Litchfield* 

26 Channel Catfish EP 22 7.1-7.3 40 13.2 (10.3-17.0) 1.33 Litchfield* 

Varied Temperature 

27 Channel Catfish TGAI 7 7.1 40 10.0 (7.3-13.6) 1.44 Litchfield* 

28 Channel Catfish TGAI 17 7.1 40 10.0 (7.3-13.8) 1.44 Litchfield* 

29 Channel Catfish TGAI 22 7.1 40 13.0 (10.2-16.6) 1.32 Litchfield* 

Varied pH 

30 Channel Catfish TGAI 22 6.5 40-42 15.0 (11.3-19.9) 1.38 Litchfield* 

31 Channel Catfish TGAI 22 7.5 40-42 13.5 (10.4-17.5) 1.34 Litchfield* 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

35

32 Channel Catfish TGAI 22 8.5 40-42 6.2 (6.2-10.8) 1.56 Litchfield* 

Varied Hardness 

33 Channel Catfish TGAI 22 8.1 40 10.0 (7.3-13.8) 1.44 Litchfield* 

34 Channel Catfish TGAI 22 8.1 320 14.0 (11.7-16.8) 1.34 Litchfield* 

Standard Tests 

35 Bluegill TGAI 22 7.1-7.3 40 13.0 

(9.9-17.1) 

1.56 Litchfield* 

36 Bluegill EP 22 7.1-7.3 40 12.0 

(8.2-17.5) 

1.83 Litchfield* 

Obs # = observation number in fluridone of M&E (1986). 

*Statistics calculated according to Litchfield and Wilcoxon (1949). 

D. VERIFICATION OF STATISTICAL RESULTS BY THE REVIEWER: TOXANAL (2009 

version) was used to calculate LC50 values; uses binomial, moving average, and probit methods. The 

probit method results were used when the probit model fit. In most cases the binomial method which 

does not require partial kills was used when there were less than two concentrations with percent dead 

between 0 and 100%. The output files from the analysis are attached at the end of this evaluation report. 

Table 16. Reviewer Calculated Statistical Endpoints. NOAEC values could not be determined because there 

were no replicates in the study. 

Observation Period 

Study Result 24 hrs 48 hrs 72 hrs 96 hrs 

Observation 13, Rainbow Trout, TGAI, pH 7.3, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L >6.4 6.4 5.1 

(4.1 – 6.4) 

5.1 

(4.1 – 6.4) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method 2 dead/10 (1) Point Estimate 

5 dead/10 (1) Binomial (1) Binomial (1)

Observation 14, Rainbow Trout, EP, pH 7.1, Hardness 40 mg/L as CaCO3, 12 oC

LC50 (95% CI) in mg a.i./L >6.3 >6.3 4.9 

(2.9 - +infinity) 

4.3 

(2.9 – 6.3) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortality (1) 1 dead/10 (1) Binomial (1) Binomial (1)

Observation 15, Rainbow Trout, TGAI, pH 7.5, Hardness 40 mg/L as CaCO3, 7 oC 

LC50 (95% CI) in mg a.i./L >10 (total) 

8.8 (total) 

(5.6-infinity 

(total)) 

8.8 (total) 

(5.6-infinity (total)) 

8.0 (total) 

(5.6-10.0 (total)) 

Probit Slope (95% CI) NA NA N/A N/A 

LC50 Method 1 dead/10 (2) Binomial (2) Binomial (2) Binomial (2)

Observation 16, Rainbow Trout, TGAI, pH 7.5, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L 10 (total) 6.8 (total) 

(5.2-9.2 (total)) 

6.0 (total) 

(4.7 - 7.8 (total)) 

4.5 (total) 

(3.2 - 5.6 (total)) 

Probit Slope (95% CI) NA 5.8 

(2.5 - 9.2) 

6.8 

(3.0 - 10.6) NA 

LC50 Method Point Estimate 

5 dead/10 (2) Probit (2) Probit (2) Binomial (2)
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

36

Observation Period 

Study Result 24 hrs 48 hrs 72 hrs 96 hrs 

Observation 17, Rainbow Trout, TGAI, pH 7.5, Hardness 40 mg/L as CaCO3, 17 oC 

LC50 (95% CI) in mg a.i./L 6.7 (total) 

(3.2 – 10 (total))

5.2 (total) 

(3.2-10 (total)) 

4.7 (total) 

(3.2-10 (total)) 

4.2 (total) 

(3.2 – 5.6 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (2) Binomial (2) Binomial (2) Binomial (2)

Observation 18, Rainbow Trout, TGAI, pH 6.5, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L >10 8.0 

(6.2-12) 

6.3 

(5.1-8.1) 

5.7 

(4.5-7.4) 

Probit Slope (95% CI) NA 6.0 

(2.0 - 9.9) 

7.1 

(3.1 - 11.2) 

6.7 

(3.0 - 10.3) 

LC50 Method 1 dead/10 (3) Probit (3) Probit (3) Probit (3)

Observation 19, Rainbow Trouth, TGAI, 

LC50 (95% CI) in mg a.i./L >10 6.7 

(5.3 - 8.5) 

6.0 

(3.2 - 10) 

5.6 

(3.2 - 10) 

Probit Slope (95% CI) NA 7.7 

(3.1 - 12.2) NA NA 

LC50 Method no mortality (3) Probit (3) Binomial (3) Binomial (3)

Observation 20, Rainbow Trout, TGAI, pH 8.5, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L >10 9.3 

(5.6 - +infinity) 

8.0 

(5.6 - 10) 

7.0 

(5.6 - 10) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortality (3) Binomial (3) Binomial (3) Binomial (3)

Observation 21, Rainbow Trout, TGAI, pH 8.1, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L >10 >10 8.7 

(6.7 - 16) 

6.7 

(5.3 - 8.5) 

Probit Slope (95% CI) NA NA 5.1 

(1.5 - 8.7) 

7.7 

(3.1 - 12.2) 

LC50 Method no mortality (3) 1 dead/10 (3) Probit (3) Probit (3)

Observation 22, Rainbow Trout, TGAI, pH 8.1, Hardness 320 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L >10 >10 8.3 

(5.6 - +infinity) 

8.0 

(5.6-10) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortality (3) 1 dead/10 (3) Binomial (3) Binomial (3)

Observation 23, Fathead Minnow, TGAI, pH 7.0, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L >18 (total) >18 (total) >18 (total) >10 (total) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortality (4) no mortality (4) no mortality (4) no mortality (4)

Observation 24, Fathead Minnow, TEP, pH 7.1, Hardness 40 mg/L as CaCO3, 22 oC

LC50 (95% CI) in mg a.i./L 8.3 

(6.7 – 10.2) 

8.3 

(6.7 – 10.2) 

8.3 

(6.7 – 10.2) 

8.3 

(6.7 – 10.2) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (1) Binomial (1) Binomial (1) Binomial (1)
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

37

Observation Period 

Study Result 24 hrs 48 hrs 72 hrs 96 hrs 

Observation 25, Channel Catfish, TGAI, pH 7.1, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (4) Binomial (4) Binomial (4) Binomial (4)

Observation 26, Channel Catfish, EP, pH 7.1, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 14.3 (total) 

(10 – 18 (total) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (4) Binomial (4) Binomial (4) Binomial (4)

Observation 27, Channel Catfish, TGAI, pH 7.1, Hardness 40 mg/L as CaCO3, 12 oC 

LC50 (95% CI) in mg a.i./L 14.3 (total) 

(10 -18 (total)) 

13 (total) 

(10 – 18 (total)) 

12 (total) 

(5.6 – 18 (total)) 10 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (5) Binomial (5) Binomial (5) Point Estimate, 5 

dead/10 (3)

Observation 28, Channel Catfish, TGAI, pH 7.1, Hardness 40 mg/L as CaCO3, 17 oC 

LC50 (95% CI) in mg a.i./L 13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (5) Binomial (5) Binomial (5) Binomial (5)

Observation 29, Channel Catfish, TGAI, pH 7.1, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L >10 14 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortality (2) Binomial (5) Binomial (5) Binomial (5)

Observation 30, Channel Catfish, TGAI, pH 6.5, Hardness 42 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L >10 13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortallity (2) Binomial (5) Binomial (5) Binomial (5)

Observation 31, Channel Catfish, TGAI, pH 7.5, Hardness 42 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L >10 13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method no mortallity (2) Binomial (5) Binomial (5) Binomial (5)

Observation 32, Channel Catfish, TGAI, pH 8.5, Hardness 42 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L >10 >10 9.3 (5.6 - +infinity) 7.5 

(6.1-9.2) 

Probit Slope (95% CI) NA NA NA 10.2 

(4.3-16.1) 

LC50 Method no mortallity (3) no mortallity (3) Binomial (3) Probit (3)
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

38

Observation Period 

Study Result 24 hrs 48 hrs 72 hrs 96 hrs 

Observation 33, Channel Catfish, TGAI, pH 8.1, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 16 (total) 

(10 – 32 (total)) 

15 (total) 

(10 – 32 (total)) 

13 (total) 

(10 – 18 (total)) 

9.3 (total) 

(5.6 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (3) Binomial (3) Binomial (3) Binomial (3)

Observation 34, Channel Catfish, TGAI, pH 8.1, Hardness 320 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 17 (total) 

(10 - +infinity) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

13 (total) 

(10 – 18 (total)) 

Probit Slope (95% CI) NA NA NA NA 

LC50 Method Binomial (5) Binomial (5) Binomial (5) Binomial (5)

Observation 35, Bluegill Sunfish, TGAI. pH 7.1, Hardness 40 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 11 (total) 

(8.5 – 14 (total))

11 (total) 

(8.5 – 14 (total))

11 (total) 

(9.0 – 14 (total)) 

11 (total) 

(9.0 – 14 (total) 

Probit Slope (95% CI) 6.5 

(2.9 – 10.1) 

6.5 

(2.9 – 10.1) NA NA 

LC50 Method Probit (5) Probit (5) Moving Average (5) Moving Average (5)

Observation 36, Bluegill Sunfish, EP, pH 7.1, Hardness 40 mg/L as CaCO3, oC 

LC50 (95% CI) in mg a.i./L 6.5 

(5.5 – 7.7) 

6.5 

(5.5 – 7.7) 

Probit Slope (95% CI) NA NA 

LC50 Method Litchfield- Wilcoxon (6) Litchfield-Wilcoxon

(6)

7/17/80, Channel Catfish, TGAI, pH 7.5, Hardness 43 mg/L as CaCO3, 22 oC 

LC50 (95% CI) in mg a.i./L 11 (total) 

(7.5 – 13 (total))

9.4 (total) 

(7.5 – 13.5 (total)) 

8.7 (total) 

(7.5 – 10 (total)) 

LC50 Method Binomial (4) Binomial (4) Binomial (2)

Abbreviations: NA=not applicable 

(1) Two measured fluridone concentrations were provided in Appendix F of the USFWS (1981) study report, and 

only these treatment levels were used to estimate the LC50 values. Fluridone concentrations averaged <79%, 

indicating nominal concentrations are not appropriate for use in characterizing the toxicity of fluridone to fish. 

(2) Measured concentrations are not available, or the available analytical results indicated that concentrations were 

not constant over time. Nominal values below known solubility (12.3 mg/L in distilled water) were used to 

calculate LC50 values, but must assume that these represent total concentrations as the solubility under study 

conditions is unknown, nor if degradation occured. 

(3) Fluridone concentrations averaged >79% of nominal. Nominal values below known solubility (12.3 mg/L in 

distilled water) were used to calculate LC50 values, with the analytical agreement these values are considered 

representative of soluble fluridone exposure concentrations. 

(4) Measured concentrations are not available, or the available analytical results indicated that concentrations were 

not constant over time. In addition to using nominal values below known solubility (12.3 mg/L in distilled water), 

the treatment just above solubility was also included because the mortality change at this dividing line was great. 

Results represent total concentrations rather than soluble as the solubility under study conditions is unknown, nor if 

degradation occurred. 

(5) Mortality occurred at the concentration just above known solubility (12.3 mg/L in distilled water). While 

fluridone concentrations averaged >79% of nominal, exposure concentrations at and above solubility are unlikely to 

have soluble concentrations near nominal (as evidenced by values that were reported for obs 13, 14, 24) and 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

39

precipitate observed in observation 25. Value above solubility was included in estimate and because of the lack of 

raw analytical data is therefore reported as total. 

(6) Fluridone concentrations averaged <79%. Authors recalculated the 96-h LC50 using measured concentrations 

rather than nominal and provided the result in Appendix F of the USFWS (1981) report. 

E. STUDY DEFICIENCIES: Study deficiencies identified by this reviewer as compared to current 

public draft OPPTS 850.1075 fish acute guidelines are discussed in Section I Materials and Methods 

under Guideline study deficiencies. 

F. REVIEWER’S COMMENTS: 

G. CONCLUSIONS: Some endpoints were scientifically sound and classified as acceptable, others had 

deficiencies and were supplemental. Confidence intervals of 96-hr LC50 values overlapped for toxicity 

tests for the rainbow trout performed at pH of 6.5, 7.5, and 8.5 indicating that pH did not influence the 

toxicity of fluridone. Confidence intervals of qualitative endpoints for the rainbow trout performed at 

different hardness (40 and 320 mg/L CaCO3) and temperatures (7, 12, and 17o

C) overlapped. This 

suggests that hardness and temperature also did not influence the toxicity of fluridone. Confidence 

intervals of the TGAI test and the EP test on the rainbow trout overlapped; however, the measured 

concentrations in the EP study were <79% nominal and it cannot be determined whether the EP was more 

toxic than the TGAI. See Table 17 for a complete summary of the reviewer determined study results. In 

several tests, only binomial 96-hr LC50 values were available because partial mortality was only observed 

at one treatment level or at no treatment levels. The slope of the dose-response curve for fluridone 

appears to be very steep indicating that the distance between the threshold concentration that produces 

effects and the concentration that will result in 100% mortality is very small. Additionally, the threshold 

of effects appears to be near the level of solubility for fathead minnow and channel catfish. This results 

in some difficulty in measuring the median lethal concentration as the exposure concentration must be 

near solubility to observe partial mortality above and below the LC50. Many of the tests had measured 

concentrations at <79% nominal. This was reportly due to storing samples too long and poor handling of 

the samples. The short aqueous photolysis half-life of fluridone indicates that it will be common for 

fluridone to degrade over the test duration. Concentrations must be measured (in TGAI studies) in order 

to verify that the exposure concentration was constant over time. 

Table 17. Summary of reviewer calculated results and study classifications 

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

13 Rainbow 

Trout, 

0.7 g 

TGAI 12 o

C, 7.3 pH, 

40, 35 

5.1 

(4.1-6.4)/ 

[N/A]/ 

Measured, Binomial 

Acceptable/ 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

40

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

14 Rainbow 

Trout, 

1.1 g 

EP 12 o

C, 7.1 pH, 

40, 35 

4.3 

(2.9-6.3)/ 

[N/A] 

Measured, Binomial 

Acceptable/ 

Varied Temperature 

15 Rainbow 

Trout, 

1.2 g 

TGAI 7 oC, 7.5 pH, 

40, 235 

8.0 (total) 

(5.6-10.0 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/ 

Analysis of test material not performed, 

sample shipment in poor condition and 

sample storage too long. 

16 Rainbow 

Trout, 

1.2 g 

TGAI 12 o

C, 7.5 pH, 

40, 235 

4.5 (total) 

(3.2-5.6 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/ 

Analysis of test material not performed, 

sample shipment in poor condition and 

sample storage too long. 

17 Rainbow 

Trout, 

1.2 g 

TGAI 17 oC, 7.5 pH, 

40, 235 

7.5 (total) 

(5.6-10.0 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/ 

Analysis of test material not performed, 

sample shipment in poor condition and 

sample storage too long. 

Varied pH 

18 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 6.5 pH, 

40, 35 

5.7 

(4.5-7.4)/ 

[6.7, 3.0-10.3] 

Nominal*, Probit 

Acceptable/ 

19 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 7.5 pH, 

40, 35 

5.6 

(3.2-10.0)/ 

[N/A] 

Nominal*,Binomial 

Acceptable/ 

20 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 8.5 pH, 

40, 35 

7.0 

(5.6-10.0)/ 

[N/A] 

Nominal*, Binomial 

Acceptable/ 

Varied Hardness 

21 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 8.1 pH, 

40, 235 

6.7 

(5.3-8.5)/ 

[7.7, 3.1-12.2] 

Nominal*, Probit 

Acceptable/ 

22 Rainbow 

Trout, 

0.98 g 

TGAI 12 oC, 8.1 pH, 

320, 235 

8.0 

(5.6-10.0)/ 

[N/A] 

Nominal*, Binomial 

Acceptable/ 

Standard Tests 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

41

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

23 Fathead 

Minnow, 

0.78 g 

TGAI 22 oC, 7.1 pH, 

40, 35 

>10 (total) 

Nominal*, no mortality 

Supplemental-qualitative/

No mortality at any treatment level below 

solubility, all mortality occurred above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis 

24 Fathead 

Minnow, 

0.78 g 

EP 22 oC, 7.1 pH, 

40, 35 

8.3 

(6.7 – 10.2)/ 

[N/A] 

Measured, Binomial 

Acceptable/ 

Standard Test 

25 Channel 

Catfish, 0.7 

g 

TGAI 22 oC, 7.1 pH, 

40, 35 

13 (total) 

(10 -18 (total)) 

[NA] 

Nominal, Binomal 

Supplemental-qualitative/ 

Water samples were lost thus no analytical 

confirmation of soluble exposure 

concentrations can be made. No mortality 

occurred at any nominal treatment level 

below solubility. Exposure concentrations at 

near solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). 

26 Channel 

Catfish, 0.7 

g 

EP 22 oC, 7.1 pH, 

40, 35 

13 (total) 

(10 -18 (total)) 

[NA] 

Nominal, Binomal 

Supplemental-qualitative/ 

Water samples were lost thus no analytical 

confirmation of soluble exposure 

concentrations can be made. No mortality 

occurred at any nominal treatment level 

below solubility. Exposure concentrations at 

near solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). 

Varied Temperature 

27 Channel 

Catfish, 0.9 

g 

TGAI 7 oC, 7.1 pH, 

40, 35 

10 

[NA] 

Nominal*, Point Estimate 

5 dead/10 

Supplemental/ 

Temperature lower than guideline 

recommendation for this species 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

42

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

28 Channel 

Catfish, 0.9 

g 

TGAI 17 oC, 7.1 pH, 

40, 35 

13 (total) 

(10 -18 (total)) 

[NA] 

Nominal, Binomal 

Supplemental-qualitative/

There was one mortality at a treatment level 

below solubility, all other mortalities 

occurred in exposure concentrations above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis. 

29 Channel 

Catfish, 0.9 

g 

TGAI 22 oC, 7.1 pH, 

40, 35 

13 (total) 

(10 -18 (total)) 

[NA] 

Nominal, Binomal 

Supplemental-qualitative/

There was one mortality at a treatment level 

below solubility, all other mortalities 

occurred in exposure concentrations above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis. 

Varied pH 

30 Channel 

Catfish, 0.4 

g 

TGAI 22 oC, 6.5 pH, 

42, 35 

13 (total) 

(10 – 18 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/

There was one mortality at a treatment level 

below solubility, all other mortalities 

occurred in exposure concentrations above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

43

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

31 Channel 

Catfish, 0.4 

g 

TGAI 22 oC, 7.5 pH, 

42, 35 

13 (total) 

(10 – 18 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/

No mortality at any treatment level below 

solubility, all mortality occurred above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis 

32 Channel 

Catfish, 0.4 

g 

TGAI 22 oC, 8.5 pH, 

42, 35 

7.5 

(6.1-9.2) 

[10.2, (4.3-16.1)] 

Nominal*, Probit 

Acceptable/ 

Included only nominal concentrations below 

the solubility limit. 

7/17/

80 

Channel 

Catfish, 1.0 

g 

TGAI 22 oC, 7.9 – 

8.65 pH, 43, 

3.5 

8.7 (total) 

(7.5-10.0 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/ 

No reported analytical sampling indicated for 

this study. Potential for soluble exposure 

levels to be lower than nominal. 

Varied Hardness 

33 Channel 

Catfish, 0.8 

g 

TGAI 22 oC, 8.1 pH, 

40, 235 

9.3 (total) 

(5.6-18 (total))/ 

[N/A] 

Nominal*, Binomial 

Supplemental-qualitative/ 

Mortality occurred near and above solubility. 

While fluridone concentration average was 

reported as >79% nominal, exposure 

concentrations at and above solubility are 

unlikely to have soluble concentrations near 

nominal (as evidenced by values that were 

reported for obs 13, 14, 24). Without actual 

reported analytical results, the results are 

qualified here as on a total basis. 

34 Channel 

Catfish, 0.8 

g 

TGAI 22 oC, 8.1 pH, 

320, 235 

13 (total) 

(10 – 18 (total))/ 

[N/A] 

Nominal, Binomial 

Supplemental-qualitative/ 

No mortality at any treatment level below 

solubility, all mortality occurred above 

solubility. While fluridone concentration 

average was reported as >79% nominal, 

exposure concentrations at and above 

solubility are unlikely to have soluble 

concentrations near nominal (as evidenced 

by values that were reported for obs 13, 14, 

24). Without actual reported analytical 

results, the results are qualified here as a total 

basis 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

44

Obs 

# 

Test 

Organism 

TGAI/ 

EP 

Temp., pH, 

Hardness and 

Alkalinity as 

mg/L CaCO3

96-hr LC50, mg a.i./L 

(95% CI)/ 

[Slope, (95% CI)] 

Basis, Statistical Method

Study Classification/ 

Comments 

Standard Tests 

35 Bluegill, 

0.5 g 

TGAI 22 oC, 7.1 pH, 

40, 35 

11 (total) 

(9.0 – 14 (total)) 

[N/A] 

Nominal, Moving Average

Supplemental-qualitative/ 

Mortality occurred at and above solubility. 

While fluridone concentration average was 

reported as >79% nominal, exposure 

concentrations at and above solubility are 

unlikely to have soluble concentrations near 

nominal (as evidenced by values that were 

reported for obs 13, 14, 24). Without actual 

reported analytical results, the results are 

qualified here as a total basis. 

36 Bluegill, 

0.5 g 

EP 22 oC, 7.1 pH, 

40, 35 

6.5 

(5.5 – 7.7)/ 

[N/C] 

Measured, LitchfieldWilcoxon 

Acceptable/ 

Measured concentrations were not provided 

but the 96-hr LC50 based on measured 

concentrations was reported in Appendix F 

of the study report. 

Obs # = M&E, 1986 study observation number for fluridone; EP=End-use product; TGAI=Technical Grade Active Ingredient; 

CI=confidence interval; NR=study not reported in M&E, 1986 

*Nominal exposure levels used in determination of endpoint but nominal concentrations were confirmed analytically (fluridone 

concentrations average > 79 percent of nominal concentrations). 

III. REFERENCES:

Committee on Methods for Toxicity Tests with Aquatic Organisms (1975). Methods for acute toxicity 

tests with fish, macroinvertebrates, and amphibians. U.S. EPA. Ecol. Res. Serv. EPA-660/3-75-

009. 61p.

Litchfield Jr., J. T., & Wilcoxon, F. 1949. A simplified method of evaluating dose-effect experiments. J. 

Pharmacol. Therap., 96, 99-113. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

45

 M and E (1986) Fluridone Obs 13 TGAI 98 percent purity RBT 72 and 96-hr LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 6.4 10 10 100 9.765625E-02 

 4.1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 4.1 AND 6.4 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 5.122499 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 14 TGAI 98 percent purity RBT 72-hr LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 6.3 10 8 80 5.46875 

 2.9 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 2.9 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 4.935012 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************ 

 M and E (1986) Fluridone Obs 14 TGAI 98 percent purity RBT 96-hr LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 6.3 10 10 100 9.765625E-02 

 2.9 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 2.9 AND 6.3 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 4.274342 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

46

 M and E (1986) Fluridone Obs 15 TGAI 98 percent purity RBT 48-h and 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 7 70 17.1875 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 8.760268 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICA STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 15 TGAI 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 9 90 1.074219 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 7.955181 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 Fluridone Obs 16 TGAI 98 percent purity RBT 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 8 80 5.46875 

 5.6 10 4 40 37.69531 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

47

 THE BINOMIAL TEST SHOWS THAT 3.2 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 6.436898 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .2148829 6.619704 5.25475 9.113658 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 6 .3300716 1 .8641161 

 SLOPE = 5.82189 

 95 PERCENT CONFIDENCE LIMITS = 2.477106 AND 9.166672 

 INTERCEPT=-4.83532 

 LC50 = 6.769262 

 95 PERCENT CONFIDENCE LIMITS = 5.258215 AND 9.172377 

 LC25 = 5.184244 

 95 PERCENT CONFIDENCE LIMITS = 3.350522 AND 6.491309 

 LC10 = 4.077618 

 95 PERCENT CONFIDENCE LIMITS = 2.022414 AND 5.251234 

 LC05 = 3.531865 

 95 PERCENT CONFIDENCE LIMITS = 1.469189 AND 4.707148 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 16 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 5 50 62.30469 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 5.600001 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 5.976884 4.838147 7.564668 

 

 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

48

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 .3126901 1 .8491775 

 SLOPE = 6.831396 

 95 PERCENT CONFIDENCE LIMITS = 3.011368 AND 10.65142 

 INTERCEPT=-5.33119 

 LC50 = 6.031086 

 95 PERCENT CONFIDENCE LIMITS = 4.747503 AND 7.756306 

 LC25 = 4.804631 

 95 PERCENT CONFIDENCE LIMITS = 3.227234 AND 5.888167 

 LC10 = 3.915534 

 95 PERCENT CONFIDENCE LIMITS = 2.127572 AND 4.924097 

 LC05 = 3.464257 

 95 PERCENT CONFIDENCE LIMITS = 1.636563 AND 4.48257 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 16 TGAI 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 9 90 1.074219 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 5.6 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 4.490551 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 17 TGAI 98 percent purity RBT 24-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 2 20 5.46875 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

49

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 6.721182 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 17 TGAI 98 percent purity RBT 48-hr LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 6 60.00001 37.69531 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 5.216388 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1906) Fluridone Obs 17 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 8 80 5.46875 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 4.69561 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

50

 M and E (1986) Floridone Obs 17 TGAI 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 10 100 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 5.6 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 4.233203 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 18 TGAI 98 percent purity RBT 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 7 70 17.1875 

 5.6 10 2 20 5.46875 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 7.968173 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 1 .8025485 7.968173 5.367896 19.70378 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 6 .4298382 1 .9863866 

 SLOPE = 5.966371 

 95 PERCENT CONFIDENCE LIMITS = 2.054696 AND 9.878046 

 INTERCEPT=-5.402087 

 LC50 = 8.043081 

 95 PERCENT CONFIDENCE LIMITS = 6.256024 AND 11.9153 

 LC25 = 6.199723 

 95 PERCENT CONFIDENCE LIMITS = 3.820752 AND 7.828876 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

51

 LC10 = 4.904769 

 95 PERCENT CONFIDENCE LIMITS = 2.0888 AND 6.295384 

 LC05 = 4.263118 

 95 PERCENT CONFIDENCE LIMITS = 1.419552 AND 5.664675 

 ************************************************************************* 

 

 M and E (1986) Fluridone Obs 18 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 4 40 37.69531 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 6.224119 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 6.2839 5.114837 8.07079 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 .3279173 1 .9512029 

 SLOPE = 7.139193 

 95 PERCENT CONFIDENCE LIMITS = 3.051001 AND 11.22739 

 INTERCEPT=-5.731041 

 LC50 = 6.349763 

 95 PERCENT CONFIDENCE LIMITS = 5.010802 AND 8.14885 

 LC25 = 5.108331 

 95 PERCENT CONFIDENCE LIMITS = 3.428347 AND 6.234012 

 LC10 = 4.199924 

 95 PERCENT CONFIDENCE LIMITS = 2.272586 AND 5.251398 

 LC05 = 3.73554 

 95 PERCENT CONFIDENCE LIMITS = 1.754007 AND 4.800982 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 18 TGAI 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 6 60.00001 37.69531 

 3.2 10 0 0 9.765625E-02 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

52

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 5.216388 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 5.684868 4.564247 7.109202 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 .3025485 1 .6696461 

 SLOPE = 6.667483 

 95 PERCENT CONFIDENCE LIMITS = 3.000073 AND 10.33489 

 INTERCEPT=-5.055319 

 LC50 = 5.730664 

 95 PERCENT CONFIDENCE LIMITS = 4.504939 AND 7.370292 

 LC25 = 4.539859 

 95 PERCENT CONFIDENCE LIMITS = 3.053294 AND 5.575872 

 LC10 = 3.681192 

 95 PERCENT CONFIDENCE LIMITS = 2.009419 AND 4.644154 

 LC05 = 3.247134 

 95 PERCENT CONFIDENCE LIMITS = 1.544174 AND 4.217266 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 19 TGAI 98 percent purity RBT 48-h LC50 and Obs 21 

RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 3 30 17.1875 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 6.711294 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 6.618923 5.404147 8.654277 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

53

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 .3472456 1 .990739 

 SLOPE = 7.6675 

 95 PERCENT CONFIDENCE LIMITS = 3.149231 AND 12.18577 

 INTERCEPT=-6.329082 

 LC50 = 6.690256 

 95 PERCENT CONFIDENCE LIMITS = 5.303757 AND 8.539605 

 LC25 = 5.463539 

 95 PERCENT CONFIDENCE LIMITS = 3.677296 AND 6.621589 

 LC10 = 4.55298 

 95 PERCENT CONFIDENCE LIMITS = 2.469681 AND 5.639651 

 LC05 = 4.082384 

 95 PERCENT CONFIDENCE LIMITS = 1.921691 AND 5.188412 

 ************************************************************************* 

 

 M and E (1986) Fluridone Obs 19 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 4 40 37.69531 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 6.027243 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 19 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 5 50 62.30469 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

54

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 5.600001 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 20 TGAI 98 percent purity RBT 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 6 60.00001 37.69531 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 9.291148 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 20 TGAI 98 percent purity RBT 72-h LC50 and Obs 22 

RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 7.955181 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

55

 M and E (1986) Fluridone Obs 20 TGAI 98 percent purity RBT 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 10 100 9.765625E-02 

 5.6 10 1 10 1.074219 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 7.039438 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 21 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 6 60.00001 37.69531 

 5.6 10 2 20 5.46875 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 3.2 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 8.699842 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 1 1.25215 8.699842 4.888956 +INFINITY 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 6 .4923888 1 .9683006 

 SLOPE = 5.134785 

 95 PERCENT CONFIDENCE LIMITS = 1.531685 AND 8.737886 

 INTERCEPT=-4.826815 

 LC50 = 8.710094 

 95 PERCENT CONFIDENCE LIMITS = 6.668144 AND 16.01429 

 LC25 = 6.436709 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

56

 95 PERCENT CONFIDENCE LIMITS = 3.802978 AND 8.527691 

 LC10 = 4.902653 

 95 PERCENT CONFIDENCE LIMITS = 1.721019 AND 6.446584 

 LC05 = 4.165608 

 95 PERCENT CONFIDENCE LIMITS = 1.025372 AND 5.694646 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 22 TGAI 98 percent purity RBT 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 8 80 5.46875 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 8.331868 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 

 M and E (1986) Fluridone Obs 23 TGAI 98 percent purity FHM 24- ,48- and 72-h 

LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 32 10 10 100 9.765625E-02 

 18 10 0 0 9.765625E-02 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 18 AND 32 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 23.99999 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 23 TGAI 98 percent purity FHM 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

57

 32 10 10 100 9.765625E-02 

 18 10 1 10 1.074219 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 18 AND 32 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 22.58704 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 *********************************************************************** 

 M and E (1986) Floridone Obs 24 EP 48 percent purity FHM 24-, 48-, 72-, and 96-

h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10.2 10 10 100 9.765625E-02 

 6.7 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 6.7 AND 10.2 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 8.2668 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ******************************************************************** 

M and E (1986) Fluridone Obs 25 TGAI 98 percent purity CCF 24-, 48-, 72- and 96-h 

LC50; (Obs 26 CCF 48-, 72-, and 96-h LC50; Obs 27 48-h LC50; Obs 28 24- and 48-h 

LC50; Obs 29 72- and 96-h LC50; Obs 30 72-h LC50; Obs 31 48-, 72-, and 96-h 

LC50; Obs 34 48-, 72-h, and 96-h LC50) 

(Obs 34) 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 13.41641 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

58

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************ 

 Fluridone Obs 27 TGAI 98 percent purity CCF 24-h LC50 (Obs 29 48-h LC50) 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 9 90 1.074219 

 10 10 0 0 9.765625E-02 

 5.6 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 14.27438 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 27 TGAI 98 percent purity CCF 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 2 20 5.46875 

 5.6 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 12.03225 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 27 TGAI 98 percent purity CCF 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 5 50 62.30469 

 5.6 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

59

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 10 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************ 

 Fluridone Obs 28 TGAI 98 percent purity CCF 72- and 96-h LC50 (Obs 30 96-h 

LC50) 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 1 10 1.074219 

 5.6 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 12.61001 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************ 

 M and E (1986) Fluridone Obs 32 TGAI 98 percent purity CCF 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 6 60.00001 37.69531 

 5.6 10 0 0 9.765625E-02 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 .56 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND +INFINITY CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 9.291148 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

60

 M and E (1986) Fluridone Obs 32 TGAI 98 percent purity CCF 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 10 10 9 90 1.074219 

 5.6 10 1 10 1.074219 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 1 10 0 0 9.765625E-02 

 .56 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 7.483314 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 7.483314 6.097914 10.30101 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 .335756 1 .9999999 

 SLOPE = 10.19018 

 95 PERCENT CONFIDENCE LIMITS = 4.285535 AND 16.09482 

 INTERCEPT=-8.907669 

 LC50 = 7.484155 

 95 PERCENT CONFIDENCE LIMITS = 6.085822 AND 9.219152 

 LC25 = 6.426161 

 95 PERCENT CONFIDENCE LIMITS = 4.657015 AND 7.613862 

 LC10 = 5.602405 

 95 PERCENT CONFIDENCE LIMITS = 3.496446 AND 6.709696 

 LC05 = 5.160867 

 95 PERCENT CONFIDENCE LIMITS = 2.916097 AND 6.283215 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 33 TGAI 98 percent purity CCF 24-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 32 10 10 100 9.765625E-02 

 18 10 7 70 17.1875 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND 32 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 15.73982 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

61

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 33 TGAI 98 percent purity CCF 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 32 10 10 100 9.765625E-02 

 18 10 8 80 5.46875 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND 32 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 14.95979 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 33 TGAI 98 percent purity CCF 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 6 60.00001 37.69531 

 5.6 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 9.291148 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************ 

 M and E (1986) Fluridone Obs 34 TGAI 98 percent purity CCF 24-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 6 60.00001 37.69531 

 10 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 10 AND +INFINITY CAN BE 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

62

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 16.70717 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 35 TGAI 98 percent purity BG 24 and 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 2 20 5.46875 

 5.6 10 1 10 1.074219 

 3.2 10 0 0 9.765625E-02 

 1.8 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 12.03225 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 11.08247 8.965049 14.2681 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 8 .3107925 1 .2334879 

 SLOPE = 6.497675 

 95 PERCENT CONFIDENCE LIMITS = 2.875301 AND 10.12005 

 INTERCEPT=-6.747158 

 LC50 = 10.92435 

 95 PERCENT CONFIDENCE LIMITS = 8.525309 AND 14.18352 

 LC25 = 8.6018 

 95 PERCENT CONFIDENCE LIMITS = 5.687832 AND 10.62462 

 LC10 = 6.936749 

 95 PERCENT CONFIDENCE LIMITS = 3.674683 AND 8.808607 

 LC05 = 6.09879 

 95 PERCENT CONFIDENCE LIMITS = 2.791151 AND 7.981655 

 ************************************************************************* 

 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

63

 M and E (1986) Fluridone Obs 35 TGAI 98 percent purity BG 72- and 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 10 10 2 20 5.46875 

 5.6 10 1 10 1.074219 

 3.2 10 0 0 9.765625E-02 

 1.8 10 1 10 1.074219 

 1 10 0 0 9.765625E-02 

 .56 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 5.6 AND 18 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 12.03225 

 

 

 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD 

 SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 

 2 .1677541 11.08247 8.965049 14.2681 

 

 

 RESULTS CALCULATED USING THE PROBIT METHOD 

 ITERATIONS G H GOODNESS OF FIT PROBABILITY 

 7 1.774067 3.911163 1.513898E-03 

 SINCE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED 

 USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED. 

 SLOPE = 3.173194 

 95 PERCENT CONFIDENCE LIMITS =-1.053313 AND 7.3997 

 INTERCEPT=-3.274289 

 LC50 = 10.76116 

 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY 

 LC25 = 6.596251 

 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY 

 LC10 = 4.245958 

 95 PERCENT CONFIDENCE LIMITS = 0 AND 10.23822 

 LC05 = 3.262011 

 95 PERCENT CONFIDENCE LIMITS = 0 AND 7.660581 

 *************************************************************************** 

 M and E (1986) Fluridone Obs 7-17-80 TGAI 98 percent purity CCF 72-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 13.5 10 10 100 9.765625E-02 

 10 10 7 70 17.1875 

 7.5 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 7.5 AND 13.5 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 
Data Evaluation Record on the acute toxicity of Fluridone TGAI and EP to fish (rainbow 

trout, bluegill sunfish, channel catfish, and fathead minnow)

 EPA MRID Number 00103297 and 40098001

64

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 9.364391 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 M and E (1986) Fluridone Obs 7-17-80 TGAI 98 percent purity CCF 48-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 13.5 10 10 100 9.765625E-02 

 10 10 2 20 5.46875 

 7.5 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 7.5 AND 13.5 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 10.99063 

 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 ************************************************************************* 

 Fluridone TGAI 98 percent purity 7-17-80 CCF 96-h LC50 

 ************************************************************************ 

 CONC. NUMBER NUMBER PERCENT BINOMIAL 

 EXPOSED DEAD DEAD PROB.(PERCENT) 

 18 10 10 100 9.765625E-02 

 13.5 10 10 100 9.765625E-02 

 10 10 10 100 9.765625E-02 

 7.5 10 0 0 9.765625E-02 

 

 THE BINOMIAL TEST SHOWS THAT 7.5 AND 10 CAN BE 

 USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT 

 CONFIDENCE LIMITS, BECAUSE THE ACTUAL CONFIDENCE LEVEL 

 ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT. 

 

 AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 8.660254 

 

 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE 

 PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE 

 NOR THE PROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS. 

 **************************************************