### 13339 Hagers Ferry Road Huntersville, NC 28078-7929 McGuire Nuclear Complex - MG03A2 Phone: 980-875-5245 Fax: 980-875-4349



# Order Summary Report 

# Analytical Laboratory

Order Number: J11120332

Lab Contact: Jason C Perkins 

### Date: 1/6/2012 Customer Address: 253 Plant Allen Road Customer Name(s): BILL KENNEDY, RON LAWS, ALLEN STOWE, ROBBIN JOLLY, DON SCRUG

Belmont, NC 28012

Phone: 980-875-5348 Report Authorized By: (Signature)


### Program Comments: Please contact the Program Manager (Jason C Perk ins) with any questions regarding this report. Data Flags & Calculations: Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample re sults) under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Data Package: This data package includes analytical results that are applicable only to the samples described in this narrative. An estimatio n of the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, ex cept in full, without the written consent of the Analytical Laborator y. Please contact the Analytical laboratory with any questions. The order of individual sections within this report is as follows: Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, Analytical Laboratory QC Reports, Sub- contracted Laboratory Results, Customer Specific Data Sheets, Reports & Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC) Certification: The Analytical Laboratory holds the following State Certificati ons : North Carolina (DENR) Certificate #248, South Carolina (DHEC) Laboratory ID # 99005. Contact t he Analytical Laboratory for definitive inform ation about the certification status of specific methods.

## Sample ID's & Descriptions:
Sample ID Plant/Station Collection Date and Time Collected By Sample Description 2011027413 ALLEN 19-Dec-11 9:46 AM Chris Williams FGD Purge Eff 2011027414 ALLEN 19-Dec-11 9:28 AM Chris Williams BioReactor 1 Inf 2011027415 ALLEN 19-Dec-11 9:10 AM Chris Williams BioReactor 1 Inf BLANK 2011027416 ALLEN 19-Dec-11 9:33 AM Chris Williams BioReactor 2 Eff 2011027417 ALLEN 19-Dec-11 9:10 AM Chris Williams BioReactor 2 Eff BLANK 2011027418 ALLEN 19-Dec-11 9:38 AM Chris Williams Filter Blk 6 Total Samples

### COC and .pdf report are in agreement with sample totals and analyses (compliance programs and procedures). All Results are less than the laboratory reporting limits. All laboratory QA/QC requirements are acceptable. The Vendor Laboratories have been qualified by the Analytical Laboratory Yes No 

# Technical Validation Review Checklist:

Yes No

Yes No



## Report Sections Included: Job Summary Report Sub-contracted Laboratory Results Sample Identification Customer Specific Data Sheets, Reports, & Documentation Technical Validation of Data Package Customer Database Entries Analytical Laboratory Certificate of Analysis Analytical Laboratory QC Report Chain of Custody

Reviewed By: 

### Mary Ann Ogle Date: 

### 1/6/2012 Yes

Electronic Data Deliverable (EDD) Sent Separately

# Certificate of Laboratory Analysis This rep ort shall not be re p roduced, exce p t in full. 

## Order # J11120332

2011027413 Collection Date: 19-Dec-11 9:46 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst ALKALINITY (FIXED END POINT 4.5) Vendor Parameter COMPLETE V_PRISM Carbonate, Bicarbonate, and Hydroxide Alkalinity Bicarbonate (HCO3) COMPLETE V_PRISM Carbonate (CO3) COMPLETE V_PRISM Hydroxide (OH) COMPLETE V_PRISM NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) 100 mg-N/L EPA 353.2 21-Dec-11 11:03 BGN9034 1 INORGANIC IONS BY IC Bromide 29 mg/L EPA 300.0 29-Dec-11 00:45 JAHERMA 5 Chloride 2200 mg/L EPA 300.0 29-Dec-11 00:45 JAHERMA 100 Sulfate 2500 mg/L EPA 300.0 29-Dec-11 00:45 JAHERMA 100 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 109 ug/L EPA 245.1 30-Dec-11 08:54 AGIBBS 2.5 Mercury Dissolved (cold vapor) in Water (Filtered) Mercury (Hg) 65.6 ug/L EPA 245.1 30-Dec-11 11:40 AGIBBS 2.5 TOTAL RECOVERABLE METALS BY ICP Boron (B) 213 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 Calcium (Ca) 2490 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.10 Cobalt (Co) 0.092 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Lithium (Li) 0.335 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Magnesium (Mg) 1180 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Potassium (K) 73.7 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0 Sodium (Na) 47.8 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 DISSOLVED METALS BY ICP-MS Selenium (Se) 1600 ug/L EPA 200.8 27-Dec-11 11:09 MHH7131 10

# Certificate of Laboratory Analysis This rep ort shall not be re p roduced, exce p t in full. 

## Order # J11120332

2011027413 Collection Date: 19-Dec-11 9:46 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst TOTAL RECOVERABLE METALS BY ICP-MS Antimony (Sb) <10 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Arsenic (As) 197 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Beryllium (Be) <10 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Cadmium (Cd) <10 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Chromium (Cr) 202 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Copper (Cu) 131 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Lead (Pb) 151 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Nickel (Ni) 246 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Selenium (Se) 1490 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Silver (Ag) <10 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 10 Zinc (Zn) 213 ug/L EPA 200.8 29-Dec-11 14:26 DJSULL1 20 SELENIUM SPECIATION Vendor Parameter COMPLETE V_AS&C TOTAL DISSOLVED SOLIDS TDS 9100 mg/L SM2540C 10 TOTAL SUSPENDED SOLIDS TSS 3800 mg/L SM2540D 22-Dec-11 11:30 TJA7067 5 

### 2011027414 Collection Date: 19-Dec-11 9:28 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst ALKALINITY (FIXED END POINT 4.5) Vendor Parameter COMPLETE V_PRISM Carbonate, Bicarbonate, and Hydroxide Alkalinity Hydroxide (OH) COMPLETE V_PRISM Carbonate (CO3) COMPLETE V_PRISM Bicarbonate (HCO3) COMPLETE V_PRISM NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) 110 mg-N/L EPA 353.2 21-Dec-11 11:05 BGN9034 1 INORGANIC IONS BY IC Bromide 46 mg/L EPA 300.0 29-Dec-11 01:01 JAHERMA 5 Chloride 2800 mg/L EPA 300.0 29-Dec-11 01:01 JAHERMA 100 Sulfate 2300 mg/L EPA 300.0 29-Dec-11 01:01 JAHERMA 100

# Certificate of Laboratory Analysis This rep ort shall not be re p roduced, exce p t in full. 

## Order # J11120332

2011027414 Collection Date: 19-Dec-11 9:28 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst MERCURY 1631 Vendor Parameter COMPLETE V_BRAND MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 76.6 ug/L EPA 245.1 30-Dec-11 08:56 AGIBBS 2.5 TOTAL RECOVERABLE METALS BY ICP Boron (B) 213 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 Calcium (Ca) 1580 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.10 Cobalt (Co) <0.05 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.05 Lithium (Li) 0.138 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Magnesium (Mg) 1100 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Potassium (K) 29.6 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0 Sodium (Na) 49.7 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 TOTAL RECOVERABLE METALS BY ICP-MS Antimony (Sb) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Arsenic (As) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Beryllium (Be) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Cadmium (Cd) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Chromium (Cr) 36.4 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Copper (Cu) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Lead (Pb) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Nickel (Ni) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Selenium (Se) 1510 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Silver (Ag) <10 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 10 Zinc (Zn) <20 ug/L EPA 200.8 29-Dec-11 14:29 DJSULL1 20 SELENIUM SPECIATION Vendor Parameter COMPLETE V_AS&C 

### 2011027415 Collection Date: 19-Dec-11 9:10 AM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst MERCURY 1631 Vendor Parameter COMPLETE V_BRAND

# Certificate of Laboratory Analysis This rep ort shall not be re p roduced, exce p t in full. 

## Order # J11120332

2011027416 Collection Date: 19-Dec-11 9:33 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst ALKALINITY (FIXED END POINT 4.5) Vendor Parameter COMPLETE V_PRISM Carbonate, Bicarbonate, and Hydroxide Alkalinity Hydroxide (OH) COMPLETE V_PRISM Carbonate (CO3) COMPLETE V_PRISM Bicarbonate (HCO3) COMPLETE V_PRISM NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) 3.6 mg-N/L EPA 353.2 21-Dec-11 11:08 BGN9034 0.1 INORGANIC IONS BY IC Bromide 54 mg/L EPA 300.0 29-Dec-11 01:16 JAHERMA 5 Chloride 2800 mg/L EPA 300.0 29-Dec-11 01:16 JAHERMA 100 Sulfate 2800 mg/L EPA 300.0 29-Dec-11 01:16 JAHERMA 100 MERCURY 1631 Vendor Parameter COMPLETE V_BRAND MERCURY (COLD VAPOR) IN WATER Mercury (Hg) <1 ug/L EPA 245.1 30-Dec-11 08:59 AGIBBS 1.0 TOTAL RECOVERABLE METALS BY ICP Boron (B) 197 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 Calcium (Ca) 1540 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.10 Cobalt (Co) <0.05 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Lithium (Li) 0.164 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Magnesium (Mg) 1160 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050 Potassium (K) 75.7 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0 Sodium (Na) 55.6 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50 TOTAL RECOVERABLE METALS BY ICP-MS Antimony (Sb) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Arsenic (As) 5.41 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Beryllium (Be) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Cadmium (Cd) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Chromium (Cr) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Copper (Cu) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Lead (Pb) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Nickel (Ni) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Selenium (Se) 13.2 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Silver (Ag) <5 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 5 Zinc (Zn) <10 ug/L EPA 200.8 29-Dec-11 13:57 DJSULL1 10

# Certificate of Laboratory Analysis This rep ort shall not be re p roduced, exce p t in full. 

## Order # J11120332

2011027416 Collection Date: 19-Dec-11 9:33 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst SELENIUM SPECIATION Vendor Parameter COMPLETE V_AS&C 

### 2011027417 Collection Date: 19-Dec-11 9:10 AM 

### Site: BioReactor 2 Eff BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst MERCURY 1631 Vendor Parameter COMPLETE V_BRAND 

### 2011027418 Collection Date: 19-Dec-11 9:38 AM 

### Site: Filter Blk Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sam p le #:

Analyst DISSOLVED METALS BY ICP-MS Selenium (Se) <1 ug/L EPA 200.8 27-Dec-11 11:03 MHH7131 1

## January 3 , 2012

Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 (704) 875-5 245 Project: HAPS/MACT Testing Allen (LIMS # J11120332 )

Dear Mr. Perkins, Attached is the report associated with three (3 ) aqueous samples submitted for selenium speciation analysis on December 22 , 2011. The samples were received in a sealed cooler at- 0. 3 ° C on December 23, 2011. Selenium speciation analysis was performed via ionchromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICPDRC-MS). Any issues associated with the analysis are addressed in the following report. If you have any questions, please feel free to contact me at your convenience.
Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

## Applied Speciation and Consulting, LLC Report prepared for: Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 Project: HAPS/MACT Testing Allen (LIMS # J111203 32 )

January 3 , 2012

1. Sample Reception Three (3 ) aqueous samples in125mL HDPE bottles (provided by Applied Speciation and Consulting) were submitted for selenium speciation analysi s on December 22 , 2011 . The samples were received on December 2 3 , 2011 in a sealed container at -0. 3° C. The samples were received in a laminar flow clean hood, void of trace metals contamination and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each sample was filtered (0.45μm) and each filtrate w as stored in a secure, monitored cryofreezer (maintained at a temperature of -80 o 

## C) until selenium speciation analysis could be performed via ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS).

2. Sample Preparation All sample preparation is performed in laminar flow clean hoods known to be free from trace metals contamination. All applied water for dilutions and sample preservatives are monitored for contamination to account for any biases associated with the sample results. Selenium Speciation Analysis by IC -ICP-DRC

- MS Prior to analysis, an aliquot of each sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed autosampler vial. No further sample preparation was performed as any chemical alteration of a sample may shift the equilibrium of the system, resulting in changes in speciation ratios.

3. Sample Analysis All sample analysis is preceded by a minimum of a five-point calibration curve spanning the entire concentration range of interest. Calibration curves are performed at the beginning of

## each analytical day. All calibration curves, associated with each species of interest, are

standardized by linear regression resulting in a response factor. All sample results are instrument blank corrected to account for any operational biases associated with the analytical platform. Prior to sample analysis, all calibration curves are verified using second source standard s which are identified as initial calibration verification standards (ICV). Ongoing instrument performance is identified by the analysis of continuing calibration verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval of every ten analytical runs. Selenium Speciation Analysis by IC -ICP-DRC

- MS Eachsample for selenium speciation analysis w as analyzed by ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS) on December 29 , 2011 . An aliquot of each sample is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio frequency (RF ) plasma where energy-transfer processes cause desolvation, atomization, and ionization. The ions are extracted from the plasma through a differentially-pumped vacuum interface and travel through a pressurized chamber (DRC) containing a specific reactive gas which preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions transmitted through the mass analyzer and the resulting current is processed by a data handling system. Retention times for each eluting species are compared to known standards for species identification.

4. Analytical Issues The overall analyses went well and no significant analytical issues were encountered. All quality control parameters associated with this sample were within acceptance limits. The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are generated from replicate analyses of the lowest standard in the calibration curve. Not all selenium species are present in preparation blanks; therefore, eMDL calculations based on preparation blanks are artificially biased low. The eMDL for methylseleninic acid and selenomethionine is calculated from the average eMDL of selenite, selenate, and selenocyanate. The calibration does not contain methylseleninic acid or selenomethionine due to impurities in these standards which would bias the results for other selenium species.

## If you have any questions or concerns regarding this report, please feel free to contact me.

Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

### Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe Unknown Se Species (n) FGD Purge Eff ND (<3.0) 1500 ND (<5.9) ND (<4.0) ND (<4.0) 0 (0) BioReactor 1 Inf 2.29 1490 ND (<1.5) ND (<1.0) ND (<1.0) 1.0 (1) BioReactor 2 Eff 7.10 5.98 ND (<1.5) ND (<1.0) ND (<1.0) 0 (0) All results reflect the applied dilution and are reported in μg/L ND = Not detected at the applied dilution SeCN = Selenocyanate MeSe(IV) = Methylseleninic acid SeMe = Selenomethionine Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS n = number of unknown Se species observed Report Generated by: Russell Gerads Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins
Date: January 3, 2012 LIMS #J11120332

### Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 50x eMDL 200x Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.74 3.0 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.79 3.2 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.030 1.5 5.9 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.020 1.0 4.0 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.020 1.0 4.0 eMDL = Estimated Method Detection Limit *Please see narrative regarding eMDL calculations
Quality Control Summary - Certified Reference Materials Analyte (μg/L) CRM True Value Result Recovery Se(IV) LCS 9.57 9.72 101.5 Se(VI) LCS 9.48 9.00 94.9 SeCN LCS 8.92 9.09 101.9 MeSe(IV) LCS 6.47 6.17 95.3 SeMe LCS 9.32 9.14 98.1 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins LIMS #J11120332 Date: January 3, 2012 Report Generated by: Russell Gerads

### Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD Se(IV) Batch QC ND (<3.0) ND (<3.0) NC NC Se(VI) Batch QC 1654 1617 1636 2.2 SeCN Batch QC ND (<5.9) ND (<5.9) NC NC MeSe(IV) Batch QC ND (<4.0) ND (<4.0) NC NC SeMe Batch QC ND (<4.0) ND (<4.0) NC NC ND = Not detected at the applied dilution NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD Se(IV) Batch QC 1112 1166 104.8 1112 1185 106.6 1.7 Se(VI) Batch QC 1009 2584 94.0 1009 2600 95.6 0.6 SeCN Batch QC 915.0 829.1 90.6 915.0 812.3 88.8 2.0 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins LIMS #J11120332 Date: January 3, 2012 Report Generated by: Russell Gerads

### 3958 6 th 

### Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

## January 3, 2012
Duke Energy ATTN: Jay Perkins Scientific Support-Laboratory 13339 Hagers Ferry Road Huntersville NC 28078 jcperkins@duke-energy.com labcustomer@duke-energy.com
RE: Project DUK-HV1101 Client Project: J11120332 Dear Mr. Perkins, On December 23, 2011, Brooks Rand Labs (BRL) received two (2) waste water samples and two (2) corresponding field blanks. Samples were logged-in for total mercury (Hg) analysis. All samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA methodology. The results were blank-corrected as described in the calculations section of the applicable SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. Please refer to the Sample Results page for sample-specific detection limits and other details. Aside from concentration qualifiers, all data was reported without qualification and all associated quality control sample results met the acceptance criteria. BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is NELAP accredited meet all NELAP requirements. For more details, see the Report Information page of the report. Please feel free to contact me if you have any questions regarding this report.
Sincerely,

Lydia Greaves Project Manager lydia@brooksrand.comProject ID: 

### DUK-HV1101

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



### Definition of Data Qualifiers

(Effective 9/23/09) 

### Laboratory Accreditation BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the samples listed in the report. 

# Report Information

BLK BRL BS CAL CCV CRM D DUP ICV MSD ND NR PS REC RPD RSD SCV SOP 

### method blank Brooks Rand Labs laboratory fortified blank calibration standard continuing calibration verification certified reference material dissolved fraction duplicate initial calibration verification matrix spike duplicate non-detect non-reportable post preparation spike percent recovery relative percent difference relative standard deviation secondary calibration verification standard operating procedure MDL MRL MS



### method detection limit method reporting limit matrix spike

SRM T COC



### standard reference material total recoverable fraction chain of custody record 

### Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL. Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate. B E 

### An estimated value due to the presence of interferences. A full explanation is presented in the narrative. H 

### Holding time and/or preservation requirements not met. Result is estimated. J 

### Estimated value. A full explanation is presented in the narrative. J-M 

### Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated. J-N 

### Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated. M 

### Duplicate precision (RPD) was not within acceptance criteria. Result is estimated. N 

### Spike recovery was not within acceptance criteria. Result is estimated. R 

### Rejected, unusable value. A full explanation is presented in the narrative. U 

### Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL. X 

### Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. Result is estimated. 

### Field Quality Control Samples Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1101

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Information

Report Matrix Type Received Sampled Sample Lab ID 

### 1152031-01 BioReactor 1 Inf 12/19/2011 12/23/2011 Sample Influent 1152031-02 BioReactor 1 Inf Hg Blk 12/19/2011 12/23/2011 Field Blank DIW 1152031-03 BioReactor 2 Eff 12/19/2011 12/23/2011 Sample Effluent 1152031-04 BioReactor 2 Eff Hg Blk 12/19/2011 12/23/2011 Field Blank DIW 

# Batch Summary

Analyte Prepared Analyzed Sequence Batch Lab Matrix Method 

### B112204 12/28/2011 01/02/2012 1200001 Water EPA 1631 Hg



# Sample Results

Sample Sequence Result MDL MRL Unit Batch

Qualifier Analyte Fraction

Report Matrix BioReactor 1 Inf 

### ng/L 78200 T 1200001 B112204 Influent 808 303 1152031-01 Hg
BioReactor 1 Inf Hg Blk 

### ng/L 0.15 T U 1200001 B112204 DIW 0.41 0.15 1152031-02 Hg

BioReactor 2 Eff 

### ng/L 9.22 T 1200001 B112204 Effluent 1.94 0.73 1152031-03 Hg
BioReactor 2 Eff Hg Blk 

### ng/L 0.15 T U 1200001 B112204 DIW 0.40 0.15 1152031-04 Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1101

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B112204

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1153040, NIST 1641d 1000x dilution) B112204-SRM1 

### 15.68 107% ng/L 85-115 Hg 16.75
Matrix Spike (1152032-03) B112204-MS3 

### 48.54 93% ng/L 71-125 Hg 56.28

11.34
Matrix Spike Duplicate (1152032-03) B112204-MSD3 

### 49.18 108% ng/L 71-125 2414% Hg 64.45

11.34



# Method Blanks & Reporting Limits

Batch: 

### B112204
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B112204-BLK1 ng/L 0.03 B112204-BLK2 ng/L 0.06 B112204-BLK3 ng/L 0.06 B112204-BLK4 ng/L 0.03 MDL: 

### 0.15 Average: 

### 0.05 Standard Deviation: 

### 0.02 Limit: 

### 0.10 Limit: 

### 0.50 MRL: 

### 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1101

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200001 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 01/02/2012 Total Mercury and Mercury Speciation by CVAFS

Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200001-IBL1 5.97 pg of Hg 1200001-IBL2 10.23 pg of Hg 1200001-IBL3 5.33 pg of Hg 1200001-IBL4 5.20 pg of Hg 1200001-CAL1 25.00 24.46 pg of Hg 98% 1200001-CAL2 100.0 102.3 pg of Hg 102% 1200001-CAL3 500.0 476.6 pg of Hg 95% 1200001-CAL4 2500 2587 pg of Hg 103% 1200001-CAL5 10000 10160 pg of Hg 102% 1200001-ICV1 1568 1675 pg of Hg 107% 85-115 1200001-CCB1 10.1 pg of Hg 1200001-CCV1 500.0 510.9 pg of Hg 102% 77-123 1200001-CCV2 500.0 471.0 pg of Hg 94% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1101

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1152031-01 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 12/23/2011 Sample: 

### BioReactor 1 Inf Collected: 

### 12/19/2011 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71470160 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1152031-02 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 12/23/2011 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 12/19/2011 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71470160 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1152031-03 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 12/23/2011 Sample: 

### BioReactor 2 Eff Collected: 

### 12/19/2011 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71470160 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1152031-04 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 12/23/2011 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 12/19/2011 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71470160 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T



# Shipping Containers

Cooler Tracking No: 

### 4726 7966 7029 via FedEx Temperature: 

### ambient Coolant Type: 

### None Description: 

### Cooler Damaged in transit? 

### No Returned to client? 

### No Custody seals present? 

### No Custody seals intact? 

### No COC present? 

### Yes Received: 

### December 23, 2011 9:30

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed according to the referenced methods. Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case narrative. NC Certification No. 402 SC Certification No. 99012 NC Drinking Water Cert No. 37735 

# Case Narrative 12/29/2011

Duke Energy Corporation (04) Jay Perkins 13339 Hagers Ferry Road Huntersville, NC 28078 Project: HAPS/MACT Testing Allen Project No.: J11120332 Lab Submittal Date: 12/20/2011 Prism Work Order: 1120562

Please call if you have any questions relating to this analytical report. Respectfully, PRISM LABORATORIES, INC.



### VP Laboratory Services Reviewed By

Data Qualifiers Key Reference: 

### HT Sample received and analyzed outside of the hold time.

Method Detection Limit MDL Below Reporting Limit BRL
Relative Percent Difference RPD


## * Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 1 of 8

# Sample Receipt Summary 12/29/2011 

### Prism Work Order: 1120562 Client Sample ID Matrix Lab Sample ID Date Sampled Date Received 

### 1120562-01 12/19/11 2011027413/FGD Purge EFF 12/20/11 Water 1120562-02 12/19/11 2011027414/BioReactor 1 Inf 12/20/11 Water 1120562-03 12/19/11 2011027416/BioReactor 2 Eff 12/20/11 Water
Samples received in good condition at 0.5 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 2 of 8

# Laboratory Report 12/29/2011

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Allen Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 1120562-01 Prism Work Order: 1120562 Time Collected: 12/19/11 09:46 Time Submitted: 12/20/11 16:10 2011027413/FGD Purge EFF


### J11120332

General Chemistry Parameters 7.2 *SM4500-H B P1L0490 1 12/21/11 9:15 JAB HT pH Units pH 67 *SM2320 B P1L0529 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Total Alkalinity BRL *SM2320 B P1L0530 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Carbonate Alkalinity 67 *SM2320 B P1L0531 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 3 of 8

# Laboratory Report 12/29/2011

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Allen Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 1120562-02 Prism Work Order: 1120562 Time Collected: 12/19/11 09:28 Time Submitted: 12/20/11 16:10 2011027414/BioReactor 1 Inf


### J11120332

General Chemistry Parameters 7.6 *SM4500-H B P1L0490 1 12/21/11 9:15 JAB HT pH Units pH 84 *SM2320 B P1L0529 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Total Alkalinity BRL *SM2320 B P1L0530 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Carbonate Alkalinity 84 *SM2320 B P1L0531 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 4 of 8

# Laboratory Report 12/29/2011

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Allen Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 1120562-03 Prism Work Order: 1120562 Time Collected: 12/19/11 09:33 Time Submitted: 12/20/11 16:10 2011027416/BioReactor 2 Eff


### J11120332

General Chemistry Parameters 7.9 *SM4500-H B P1L0490 1 12/21/11 9:15 JAB HT pH Units pH 390 *SM2320 B P1L0529 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Total Alkalinity BRL *SM2320 B P1L0530 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Carbonate Alkalinity 390 *SM2320 B P1L0531 1 5.0 12/22/11 11:15 JAB mg/L 1.4 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 5 of 812/29/11 

### Level II QC Report

13339 Hagers Ferry Road Project No: J11120332 Project: HAPS/MACT Testing Allen Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 1120562 Time Submitted: 12/20/2011 4:10:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P1L0490 - NO PREP LCS (P1L0490-BS1) Prepared & Analyzed: 12/21/11 pH pH Units 6.87 6.860 99-101 100 Batch P1L0529 - NO PREP Blank (P1L0529-BLK1) Prepared & Analyzed: 12/22/11 Total Alkalinity mg/L BRL 5.0 LCS (P1L0529-BS1) Prepared & Analyzed: 12/22/11 Total Alkalinity mg/L 256 5.0 250.0 90-110 102 LCS Dup (P1L0529-BSD1) Prepared & Analyzed: 12/22/11 Total Alkalinity mg/L 257 5.0 250.0 200 90-110 103 0.4 Duplicate (P1L0529-DUP1) Prepared & Analyzed: 12/22/11 Source: 1120562-02 Total Alkalinity mg/L 86.6 5.0 84.0 20 3 Batch P1L0530 - NO PREP Blank (P1L0530-BLK1) Prepared & Analyzed: 12/22/11 Carbonate Alkalinity mg/L BRL 5.0 LCS (P1L0530-BS1) Prepared & Analyzed: 12/22/11 Carbonate Alkalinity mg/L 256 5.0 90-110 LCS Dup (P1L0530-BSD1) Prepared & Analyzed: 12/22/11 Carbonate Alkalinity mg/L 257 5.0 200 90-110 0.4

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 6 of 812/29/11 

### Level II QC Report

13339 Hagers Ferry Road Project No: J11120332 Project: HAPS/MACT Testing Allen Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 1120562 Time Submitted: 12/20/2011 4:10:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P1L0530 - NO PREP Duplicate (P1L0530-DUP1) Prepared & Analyzed: 12/22/11 Source: 1120562-02 Carbonate Alkalinity mg/L BRL 5.0 BRL 20 Batch P1L0531 - NO PREP Blank (P1L0531-BLK1) Prepared & Analyzed: 12/22/11 Bicarbonate Alkalinity mg/L BRL 5.0 LCS (P1L0531-BS1) Prepared & Analyzed: 12/22/11 Bicarbonate Alkalinity mg/L 256 5.0 250.0 90-110 102 LCS Dup (P1L0531-BSD1) Prepared & Analyzed: 12/22/11 Bicarbonate Alkalinity mg/L 257 5.0 250.0 200 90-110 103 0.4 Duplicate (P1L0531-DUP1) Prepared & Analyzed: 12/22/11 Source: 1120562-02 Bicarbonate Alkalinity mg/L 86.6 5.0 84.0 20 3

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 7 of 8Page 8 of 8