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TABLE OF CONTENTS

I. PRELIMINARY WORK PLAN ........................................................................................... 4

A. Overview ..................................................................................................................... 4

B. Statutory and Regulatory Authority ............................................................................. 4

C. Background and Regulatory Information ..................................................................... 5

D. Anticipated Data Needs and Risk Assessments ............................................................. 9

E. Risk to Threatened and Endangered Species .............................................................. 12

F. Endocrine Effects ....................................................................................................... 14

G. Incidents .................................................................................................................... 14 

I. Guidance for Commenters ......................................................................................... 15

J. Next Steps .................................................................................................................. 17

II. BIBLIOGRAPHY.............................................................................................................. 18

A. Studies Supporting Registration Review of 1-MCP ..................................................... 18

B. U.S. Environmental Protection Agency Regulatory Documents and Risk Assessment 

Memoranda ............................................................................................................... 37
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ACRONYMS AND ABBREVIATIONS 

AI or a.i. Active Ingredient 

BPPD The EPA’s Biopesticides and Pollution Prevention Division 

C.F.R. Code of Federal Regulations 

DCI Data Call-In 

EDSP Endocrine Disruptor Screening Program 

EPA U.S. Environmental Protection Agency 

FFDCA Federal Food, Drug, and Cosmetic Act 

FIFRA Federal Insecticide, Fungicide, and Rodenticide Act 

FQPA Food Quality Protection Act 

FR Federal Register 

FWP Final Work Plan 

IDS Incident Data System 

MRID No. Master Record Identification Number: The EPA's system of recording and tracking studies submitted to the 

Office of Pesticide Programs. 

MRL Maximum Residue Limit 

OPP The EPA’s Office of Pesticide Programs 

PC Code Pesticide chemical code: a six-digit number assigned by OPP to identify pesticide chemicals. 

PWP Preliminary Work Plan 

TGAI Technical grade of the active ingredient 

TMDL Total Maximum Daily Load 

US United States 
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I. PRELIMINARY WORK PLAN 

A. Overview 

The docket (EPA-HQ-OPP-2014-0670) for 1-methylcyclopropene (1-MCP) is now open, initiating the 

first public comment period for this registration review case (case number 6075). Sixteen products 

containing 1-MCP are currently registered for outdoor, commercial, ornamental and food use to 

counteract the effects of ethylene (ripening). This Preliminary Work Plan (PWP) explains what the U.S. 

Environmental Protection Agency’s (EPA) Office of Pesticide Programs (OPP) knows about 1-MCP, 

highlights its anticipated data and assessment needs, identifies types of information that would be 

especially useful in conducting the review, and provides an anticipated timeline for completing the 

registration review process for 1-MCP. 

The registration review process was designed to include a public participation component and, by 

sharing this information in the docket, EPA intends to inform the public of what it knows about 1-MCP 

and what types of new data or other information would be helpful to receive as it moves toward a 

decision on 1-MCP. The EPA encourages all interested stakeholders to review the PWP and to provide 

comments and additional information that will help the EPA’s decision-making process for this 

biochemical pesticide. Interested stakeholders could include the following: environmental nonprofit or 

interest groups; pesticide manufacturers; agricultural labor or commodity groups; commercial, 

institutional, residential, and other users of pesticides; or the public at large. In addition to general areas 

on which persons may wish to comment, there are some areas identified in the PWP about which the 

EPA specifically seeks comments and information. 

The PWP begins by describing the statutory and regulatory authorities for Registration Review. Next, it 

provides background on 1-MCP, which includes a description of its mode of action, the currently 

registered pesticide products, application rates and methods, use sites, and tolerance exemption 

information. Then, it lists the anticipated data needs, risk assessments, and a projected Registration 

Review timeline for this case. Finally, the PWP provides guidance for commenters, explains the next 

steps EPA will be taking, summarizes background information, and lists supporting studies, risk 

assessments, and other documents available for the registration review of 1-MCP. 

Further information about this case, including background and supporting documents, is available at 

http://www.regulations.gov (under “SEARCH for: Rules, Comments, Adjudications or Supporting 

Documents,” enter the docket identification number, which is indicated above). 

B. Statutory and Regulatory Authority

The Food Quality Protection Act (FQPA) of 1996 mandated a registration review program. All 

pesticides distributed or sold in the United States (US) must generally be registered by the EPA, based 

on scientific data showing that they will not cause unreasonable risks to human health or the 

environment when used as directed on the product labeling. The registration review program is intended 

to make sure that, as the ability to assess risk evolves and as policies and practices change, all registered 

pesticides continue to meet the statutory standard of no unreasonable adverse effects to human health or 

the environment. Changes in science, public policy, and pesticide use practices will occur over time. 

Through the registration review program, the EPA periodically reevaluates pesticides to make sure that, 
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as change occurs, products in the marketplace can continue to be used safely. Information on this 

program is provided on the EPA’s website.1

 

 

The EPA is implementing the registration review program pursuant to Federal Insecticide, Fungicide, 

and Rodenticide Act (FIFRA) section 3(g), and will review each registered pesticide every 15 years to 

determine whether it continues to meet the FIFRA standard for registration. The regulations governing 

registration review begin at 40 C.F.R. § 155.40. The EPA will consider benefits information and data as 

required by FIFRA. The public phase of registration review begins when the initial docket is opened for 

each case. The docket is the EPA’s opportunity to state what it knows about the pesticide and what 

additional risk analyses and data or information it believes are needed to make a registration review 

decision. After reviewing and responding to comments and data received in the docket during this initial 

comment period, the EPA will develop a Final Work Plan (FWP) and the anticipated schedule for the 

registration review of 1-MCP. 

 C. Background and Regulatory Information 

i. Summary of the Active Ingredient, Usage and Associated Pesticide Products 

Products containing 1-methylcyclopropene (1-MCP) are registered for use as a post-harvest tool for 

counteracting the undesirable effects of ethylene on harvested fruits, vegetables, and ornamental (such as 

cut flowers) during transport and storage, and for pre-harvest use on growing crops and ornamentals. 

Under normal environmental conditions, 1-MCP is a gas. End-use products (EPs) containing 1-MCP are 

solid (tablets or powder) or liquid formulations. In these products, 1-MCP is encapsulated in the 

formulation to enable delivery. When the formulations are applied, 1-MCP is released to the target crop. 

End-use products (EPs) containing 1-MCP are used in enclosed containers, such as coolers, truck 

trailers, greenhouses, storage facilities, and shipping containers or in the field applied via ground spray 

tanks or aerial application. 

The following table provides a summary of the chemical facts for 1-MCP: 

Table 1. Chemical Facts for 1-Methylcyclopropene (1-MCP)

PC code 224459 

Case Number 6075 

CAS Number 3100-04-7 

Year first registered 1999 

Pesticide Type Plant growth regulator 

Chemical class Biochemical 

Reregistration Eligibility 

Decision (RED) 

Not applicable. Because it was registered after November 1984, 1-

Methylcyclopropene was not subject to reregistration. 

Tolerance Reassessment 

Eligibility Decision (TRED) 

Not applicable. 1-Methylcyclopropene is exempt from the requirement for a 

tolerance. See 40 CFR § 180.1220. 

Cumulative group Not applicable. 1-Methylcyclopropene has not been identified as a member of a 

cumulative group that shares a common mechanism of toxicity. 

 

1 See http://www2.epa.gov/pesticide-reevaluation. 
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Table 1. Chemical Facts for 1-Methylcyclopropene (1-MCP)

40 CFR Citation § 180.1220: 1-Methylcyclopropene; exemption from the requirement of a 

tolerance. 

An exemption from the requirement of a tolerance is established for residues of 

the 1-Methylcyclopropene in or on fruits and vegetables when: 

(a) Used as a post-harvest plant growth regulator, i.e., for the purpose of 

inhibiting the effects of ethylene. 

(b) Applied or used outdoors for pre-harvest treatments. 

Non-pesticidal uses N/A 

The following table summarizes the use and usage information for 1-MCP: 

Table 2. 1-Methylcyclopropene (1-MCP) Use and Usage Information

Summary of Use 

1-MCP is registered for use to prolong the life of ornamental plants and cut 

flowers, as a post-harvest tool for counteracting the undesirable effects of 

ethylene on harvested fruits and vegetables during transport and storage, and 

for pre-harvest use on growing crops and ornamentals. 

Use Sites 

Ornamental plants, cut flowers, and harvested fruits and vegetables in 

enclosed indoor sites, such as coolers, truck trailers, greenhouses, storage 

facilities, and shipping containers. 

Summary of Usage EPA has no available usage data for 1-MCP. 

Formulation Types 

1-MCP is a gas at room temperature, however, end-use product formulations 

are powders, tablets and liquids. 1-MCP is released from these formulations 

with the addition of water or proprietary buffer at the time of application. 

Application Method 

Powders or tablets are mixed with water or a buffer to release 1-MCP gas in 

the presence of stored or growing commodities (ground application); liquid 

formulations are mixed with water and applied within 30 minutes to the 

commodity (ground or aerial application). 

Technical Registrant AgroFresh, Inc., a subsidiary of Rohm and Haas Company. 

No. of Registrations 16 

Restricted Use No 

The following table lists the currently registered products containing 1-MCP as their active ingredient. 

Table 3. Information About the Currently Registered 1-Methylcyclopropene (1-MCP) Pesticide Products 

EPA 

Registration 

Number 

Product Name 

(% Active Ingredient; 

Formulation Type)

Date 

Registered Application Rates Application Methods Use Sites 

71297-1 ETHYLBLOC 

(0.14%; powder) 

April 22, 

1999 

1.5 g Ethylbloc + 

1/1.5 oz Mixing 

solution per 

100/200 ft3

Apply solution directly onto 

plants and flowers 

Fresh cut flowers 

and potted 

flowering, bedding, 

nursery and foliage 

plants 

71297-2 

SMARTFRESH 

TECHNOLOGY

(3.3%; powder) 

July 17, 

2002 

Depending on crop, 

0.017- 0.067 

oz/1000ft3 Apply with use of proprietary 

generator; place appropriate 

Post-harvest fruits 

(apples, melons, 

tomatoes, pears, 

avocadoes, 

mangos, papayas, 

kiwifruit, plums, 
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Table 3. Information About the Currently Registered 1-Methylcyclopropene (1-MCP) Pesticide Products 

EPA 

Registration 

Number 

Product Name 

(% Active Ingredient; 

Formulation Type)

Date 

Registered Application Rates Application Methods Use Sites 

size Smartfresh pouch into 

water within generator 

apricots and 

persimmons) 

71297-3 

SMARTFRESH 

SMARTTABS 

(0.63%; tablets) 

March 11, 

2004 

Depending on size 

of enclosed area, 

add 1-14 tablets to 

Activator solution 

Place appropriate number of 

tablets into container 

containing Activator solution 

Food commodities 

derived from: 

apples, melons, 

tomatoes, pears, 

avocadoes, 

mangos, papayas, 

kiwifruit, plums, 

apricots and 

persimmons 

71297-4 

MANUFACTURINGUSE PRODUCT-SF 

(4.5%; powder) 

January 

30, 2004 N/A 

N/A 

For formulation 

into plant growth 

regulators (PGRs) 

that can be applied 

to apples, melons, 

tomatoes, pears, 

avocados, mangos, 

papayas, kiwifruit, 

peaches, nectarines, 

plums, apricots, 

persimmons, 

flowers and 

ornamental plants 

71297-5 

ETHYLBLOC 

SACHET 

(0.014%; sachet) 

February 

3, 2006 

Number of sachets 

depends on box 

volume (2-10) 

Dip sachets into clean water, 

place into box containing 

flowers and close lid 

Fresh cut flowers 

and potted 

flowering and 

foliage plants 

71297-6 AFXRD-038 

(3.8%; pouch) 

April 21, 

2008 

Depending on crop, 

9.4 oz/acre to 112.7 

oz/acre 

Apply through spray tank; 

adjuvant must be used 

Apples, pears, 

kiwifruit, walnut, 

tomatoes, peppers, 

corn, cotton, rice, 

soybean, 

sunflower, wheat 

71297-7 AF-600 

(1%; liquid) 

April 8, 

2009 

Depending on crop, 

36-428 fl oz/acre 

Apply through spray tank; 

aerial application also 

possible 

Apples, pears, 

kiwifruit, walnut 

71297-8 A17492F 

(1%; liquid) 

March 11, 

2010 5.7 -11.4 fl oz/acre 

Apply through spray tank; 

aerial application also 

possible 

Tomatoes, peppers, 

corn, cotton, rice, 

soybean, 

sunflower, wheat 

71297-10 

SMARTFRESH 

PROTABS 

(2%; tablets) 

March 5, 

2012 

Depending on crop 

and size of space, x 

number of tablets 

used (1 tablet treats 

1350ft3

-5300ft3) 

Place appropriate number of 

tablets into ProPack pouch 

Apples, avocadoes, 

bananas, broccoli, 

kiwifruit, melons, 

persimmons, 

tomatoes, pears, 

peaches, nectarines, 
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Table 3. Information About the Currently Registered 1-Methylcyclopropene (1-MCP) Pesticide Products 

EPA 

Registration 

Number 

Product Name 

(% Active Ingredient; 

Formulation Type)

Date 

Registered Application Rates Application Methods Use Sites 

plums, plumcots, 

apricots 

71297-11 AF-2005 

(0.8%; liquid) 

May 16, 

2012 90-470 fl oz/acre 

Apply through spray tank; 

aerial application also 

possible Apples and pears 

71297-12 

MANUFACTURINGUSE PRODUCT – EB 

– 0.14 

(0.14%; powder) 

January 

30, 2013 N/A N/A 

For formulation 

into PGRs that can 

be applied to cut 

flowers, potted 

flowers, nursery 

plants and foliage 

plants 

71297-13 

MANUFACTURINGUSE PRODUCT –SF 

– 3.3 

(3.3%; powder) 

January 

30, 2013 N/A N/A 

For formulation 

into PGRs that can 

be applied to 

apples, melons, 

tomatoes, pears, 

avocados, mangos, 

papayas, kiwifruit, 

peaches, nectarines, 

plums, apricots, 

plumcots, Asian 

pears, bananas, 

broccoli, 

persimmons, 

flowers and 

ornamental plants 

71297-14 

MANUFACTURINGUSE PRODUCT – EB 

-0.014 

(0.014%; powder) 

January 

30, 2013 N/A N/A 

For formulation 

into PGRs that can 

be applied to cut 

flowers, potted 

flowers, nursery 

plants, bedding 

plants and foliage 

plants 

71297-15 

MANUFACTURINGUSE PRODUCT – ST 

-0.63 

(0.63%; powder) 

January 

30, 2013 N/A 

N/A 

For formulation 

into PGRs that can 

be applied to 

apples, bananas, 

broccoli, melons, 

tomatoes, pears, 

Asian pears, 

avocados, kiwifruit, 

peaches, nectarines, 

plums, plumcots, 

apricots, 

persimmons, cut 

flowers, potted 
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Table 3. Information About the Currently Registered 1-Methylcyclopropene (1-MCP) Pesticide Products 

EPA 

Registration 

Number 

Product Name 

(% Active Ingredient; 

Formulation Type)

Date 

Registered Application Rates Application Methods Use Sites 

flowers, and 

bedding, nursery 

and potted plants 

71297-16 

MANUFACTURINGUSE PRODUCT – PT 

– 2.0 

(2.0%; powder) 

January 

30, 2013 N/A 

N/A 

For formulation 

into PGRs that can 

be applied to 

apples, avocados, 

bananas, broccoli, 

kiwifruit, melons, 

persimmons, 

tomatoes, pears, 

Asian pears, 

peaches, nectarines, 

plums, plumcots, 

and apricots 

71297-17 AF-701 

(1.3%; liquid) 

April 10, 

2014 

Depending on crop, 

12-242 fl oz/acre 

Ground application through 

injector nurse tank; aerial 

application also possible 

Apples, pears, 

kiwifruit 

ii. Tolerance Actions

1-MCP is exempt from the requirement of a tolerance as follows: 

40 C.F.R. § 180.1220 1-Methylcyclopropene; exemption from the requirement of tolerance: 

An exemption from the requirement of a tolerance is established for residues of the 1-

Methylcyclopropene in or on fruits and vegetables when: 

(a) Used as a post-harvest plant growth regulator, i.e., for the purpose of inhibiting the effects of 

ethylene. 

(b) Applied or used outdoors for pre-harvest treatments. 

Federal Register Citation – Final Rule 73 FR 19150, April 9, 2008. 

D. Anticipated Data Needs and Risk Assessments 

Provided below are tables of the current data requirements for biochemical pesticides as set forth in 40 

C.F.R. § 158, subpart U. They are arrayed by scientific discipline (i.e., product chemistry, human health 

assessment, and nontarget organisms) and descriptions are included regarding how the data requirements 

have been fulfilled or where data have been waived. At this time, no data gaps (anticipated data needs) 

for 1-MCP have been identified. The most recent comprehensive risk assessment for 1-MCP was 

completed February 21, 2001, in support of a request to establish an exemption from the requirement of 

a tolerance for residues of 1-MCP on all food commodities (U.S. EPA, 2001). Because there are no data 

gaps for 1-MCP or anticipated data needs, and no additional information has been received on this 
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biochemical since this most recent risk assessment, the Agency does not anticipate the need to conduct a 

new risk assessment during registration review.

i. Product Chemistry (40 C.F.R. § 158.2030) 

Table 4. Summary of Product Analysis Data for 1-Methylcyclopropene (1-MCP) 

Harmonized 

Guideline 

Number 

Data Requirement Has Data Requirement 

Been Addressed? How? 

MRID No. or Other 

Source 

880.1100 Product Identity and Composition Yes – Study 44517001 

880.1200 Description of Staring Materials, Production, 

and Formulation Process Yes – Study 44464701 

880.1400 Discussion of Formation of Impurities Yes – Study 44517002 

830.1700 Preliminary Analysis Yes – Study 44464702 

830.1750 Certified Limits Yes – Study 44517003 

830.1800 Enforcement Analytical Method Yes – Study 44464703 

Table 5. Summary of Physical and Chemical Characteristics for 1-Methylcyclopropene (1-MCP) 

Harmonized 

Guideline 

Number 

Data Requirement Has Data Requirement Been 

Addressed? How? 

MRID No. or 

Other Source 

830.6302 Color Yes – Study 44567601 

830.6303 Physical State Yes – Study 44567601 

830.6304 Odor Yes – Study 44567601 

830.6313 Stability to Normal and Elevated 

Temperatures, Metals and Metal Ions Yes – Study U.S. EPA, 2008 

830.7000 pH Yes – Study U.S. EPA, 2008 

830.7050 UV/Visible Light Absorption Yes – Study 47088604 

830.7200 Melting Point/Melting Range Not required2

830.7220 Boiling Point/Boiling Range Not required3

830.7300 Density/Relative Density/Bulk Density 

(Specific Gravity) Yes – Study U.S. EPA, 2008 

830.7520 Particle Size, Fiber Length, and Diameter 

Distribution Not required – substance is a gas. 

830.7550 

830.7560 

830.7570 

Partition Coefficient (n-Octanol Water) Not required4

 

2 Per 40 § CFR 158.2030 Test Note 13: Required when the technical chemical is a solid at room temperature. The technical grade active 

ingredient (TGAI) 1-MCP is a gas. 

3 Per 40 § CFR 158.2030 Test Note 14: Required when the technical chemical is a liquid at room temperature. 1-MCP is a gas. 4 Per 40 § CFR 158.2030 Test Note 16: Required for organic chemicals unless they dissociate in water or are partially or completely 

soluble in water. 1-MCP is partially soluble in water. 
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Table 5. Summary of Physical and Chemical Characteristics for 1-Methylcyclopropene (1-MCP) 

Harmonized 

Guideline 

Number 

Data Requirement Has Data Requirement Been 

Addressed? How? 

MRID No. or 

Other Source 

830.7840 Water Solubility Yes – Study 47088615 

830.7950 Vapor Pressure Yes – Study 45458603 

47088614 

ii. Human Health Assessment (40 C.F.R. § 158.2050) 

1-MCP exhibits low acute toxicity for all routes of exposure. It is a Toxicity Category IV for acute oral 

toxicity, acute inhalation toxicity, and primary dermal irritation, and Toxicity Category III for acute 

dermal toxicity and primary eye irritation (U.S. EPA, 2001). 1-MCP is not a skin sensitizer, and no 

hypersensitivity incidents were observed following exposure to 1-MCP. Data and information submitted 

to the Agency support the conclusions that 1-MCP is not mutagenic, not subchronically toxic, and is not 

a developmental toxicant. 

Table 6. Summary of Human Health Assessment Data for 1-Methylcyclopropene 

Harmonized 

Guideline 

Number 

Data Requirement Has Data Requirement 

Been Addressed? How? 

MRID No. or Other 

Source 

870.1100 Acute Oral Toxicity - Rat Yes – Study 44464704 

870.1200 Acute Dermal Toxicity Yes – Study 44464705 

870.1300 Acute Inhalation Toxicity – Rat Yes – Study 44464706 

870.2400 Primary Eye Irritation Yes – Study 44464707 

870.2500 Primary Dermal Irritation Yes – Study 44464708 

870.2600 Dermal Sensitization Yes – Study 44517005 

None Hypersensitivity Incidents None have been reported5

870.3100 90-Day Oral (one species) Yes – Rationale6 U.S. EPA, 2001

870.3250 90-Day Dermal – Rat Not required7

870.3465 90-Day Inhalation – Rat Yes – Study U.S. EPA, 2008

870.3700 Prenatal Developmental – Rat Preferably Yes – Study U.S. EPA, 2008 

870.5100 Bacterial Reverse Mutation Assay Yes – Study 49265008 

870.5300 

870.5375 In vitro Mammalian Cell Assay Yes – Study 49265009 

49265010

 

5 Any occurrence must be reported as an adverse incident (FIFRA § 6(a)(2)). 

6 The applicant submitted a scientifically creditable rationale in lieu of conducting a guideline study. The rationale is 

acceptable to fulfill the EPA data requirement. 7 Per 40 C.F.R. § 158.2050(e), a 90-Day Dermal study is only required to support uses involving purposeful application to the human skin 

or those which would results in comparable prolonged human exposure. 
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iii. Nontarget Organisms and Environmental Fate (40 C.F.R. § 158.2060) 

Table 7. Summary of Nontarget Organism Data for 1-Methylcyclopropene (1-MCP) 

Harmonized 

Guideline Number Data Requirement Has Data Requirement Been Addressed? How? MRID 

850.2100 Avian Acute Oral Toxicity Yes - Study 47024917 

850.2200 Avian Dietary Toxicity Yes – Study 47024918 

850.1075 Fish Acute Toxicity, Freshwater Yes - Study 47024915 

850.1010 Aquatic Invertebrate Acute Toxicity, 

Freshwater Yes - Study 47024914 

850.4100 Terrestrial Plant Toxicity, Seedling 

Emergence Yes - Rationale8

 47024921 

850.4150 Terrestrial Plant Toxicity, Vegetative 

Vigor Yes - Rationale9

 47024922 

880.4350 Nontarget Insect Testing Yes - Study 47024920 

E. Risk to Threatened and Endangered Species

Exposure of nontarget species to 1-MCP is highly unlikely for uses involving enclosed containers (such 

as coolers, truck trailers, greenhouses, storage facilities, and shipping containers). Exposure to terrestrial 

organism from outdoor uses (ground or aerial spray to apples, pears, kiwifruit, walnut, tomatoes, 

peppers, corn, cotton, rice, soybean, sunflower, or wheat) are expected to be minimal because 1-MCP 

degrades quickly: when applied at the maximum use rate of 10 gram AI per hectare, residue levels were 

0.040 mg ai/kg on the day of application and declined approximately 5-fold by 14-days post application 

(U.S. EPA, 2010). Exposure to aquatic organisms is unlikely because there are no products registered 

for aquatic use and all labels for outdoor use contain environmental hazard language to mitigate aquatic 

exposure. 

Risks to nontarget species from the pesticidal use of 1-MCP are expected to be minimal. Studies 

submitted in support of the registration and exemption for the requirement of a tolerance for residues of 

1-MCP demonstrated that this biochemical has very low mammalian toxicity (Toxicity Category IV for 

acute oral toxicity, acute inhalation toxicity, and primary dermal irritation; Toxicity Category III for 

acute dermal toxicity and primary eye irritation; not a dermal sensitizer; non-mutagenic; no subchronic 

or developmental toxicity (U.S. EPA, 2001)). Avian and non-target insect testing at high doses indicate 

that 1-MCP is practically non-toxic to these species. Aquatic organism laboratory studies tested only one 

dose level of 1-MCP and saw no mortality. The dose chosen was selected because calculations based 

upon Henry’s law constant and atmospheric pressure indicate a 1000 ppm headspace concentration of 1-

MCP to be equal to approximately 0.825 mg ai/L (the actual concentrations achieved in the test 

 

8 The applicant submitted a scientifically creditable rationale in lieu of conducting a guideline study. The rationale is 

acceptable to fulfill the EPA data requirement.

9 The applicant submitted a scientifically creditable rationale in lieu of conducting a guideline study. The rationale is 

acceptable to fulfill the EPA data requirement.
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environment were 0.766 mg ai/L for aquatic invertebrates and 0.966 mg ai/L for fish (MRIDs 47024914 

and 47024915)).These were highly conservative tests, as the concentration of 1-MCP is not expected to 

exceed 1 ppm in air during actual use conditions (U.S. EPA, 2001) and, as noted above, when applied at 

the maximum use rate, 1-MCP residues were only 0.040mg ai/kg (equivalent to approximately 0.040mg 

ai/L) on the day of application. 

Based upon the results of toxicity tests and the low potential for exposure, the risks to nontarget species, 

including endangered/threatened species and their designated critical habitats, from 1-MCP as an active 

ingredient in pesticide products are expected to be negligible. Therefore, EPA has reached a “No Effect” 

determination for endangered/threatened species and designated critical habitat. 

EPA anticipates conducting no further analysis of potential risks to endangered or threatened species, 

unless data or information are obtained during the Registration Review process that would indicate such 

an assessment would be needed to inform the Agency’s final decision concerning this case, or if future 

registrations for products containing 1-MCP as the AI are formulated in a manner that would increase 

toxicity and/or exposures above those currently registered. 

Table 8. Summary of Nontarget Organism Data Results for 1-Methylcyclopropene (1-MCP) 

Harmonized 

Guideline Number Data Requirement Results MRID 

850.2100 Avian Acute Oral Toxicity 

(Bobwhite Quail) 

LD50 > 2250 mg/kg 

practically non-toxic 47024917 

850.2200 Avian Dietary Toxicity 

(Bobwhite Quail) 

LC50 > 5620ppm 

practically non-toxic 47024918 

850.1075 Fish Acute Toxicity, Freshwater 

(Rainbow Trout) 

96-hour LC50 > 0.966 mg ai/L 

no mortality seen at highest dose 

tested (1000 times greater than 

actually use concentrations) 

47024915 

850.1010 Aquatic Invertebrate Acute Toxicity, 

Freshwater (Daphnia) 

48-hour EC50 > 0.776 mg ai/L 

no mortality seen at highest dose 

tested (1000 times greater than 

actually use concentrations) 

47024914 

850.4100 Terrestrial Plant Toxicity, 

Seedling Emergence 

Soil column data indicating that 

negligible amounts of 1-MCP will be 

deposited to soil under realistic use 

conditions and, if deposited to soil, 1-

MCP will be tightly bound to soil 

particles and thus not available to 

germinating seeds. 

47024921 

850.4150 Terrestrial Plant Toxicity, 

Vegetative Vigor 

Summary of data indicating that field 

trials with 1-MCP up to four times the 

maximum label rate showed no 

phytotoxic effects on crops including 

corn, soybean, cotton, sunflower and 

others. 

47024922 

880.4350 Nontarget Insect Testing 

48-hour LD50 > 10 ppm in air 

practically non-toxic – actual use 

concentrations not expected to exceed 

1 ppm 

47024920 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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F. Endocrine Effects 

As required by the Administrator under the Federal Food, Drug, and Cosmetic Act (FFDCA) section 

408(p), the EPA has developed the Endocrine Disruptor Screening Program (EDSP) and has begun to 

implement the screening program that is to be used to test all pesticides in order to determine whether 

certain substances (including pesticide active and other ingredients) may have an effect in humans or 

wildlife similar to an effect produced by a “naturally occurring estrogen, or other such endocrine effects 

as the Administrator may designate.” 

FFDCA section 408(p)(4) authorizes the Administrator, by order, to exempt from the requirements of 

the Estrogenic Substances Screening Program a biologic substance or other substance if a determination 

is made that the substance is not anticipated to produce any effect in humans similar to an effect 

produced by a naturally occurring estrogenic substance. 

Between October 2009 and February 2010, the EPA issued test orders/data call-ins for the first group of 

67 chemicals, which contains 58 pesticide active ingredients and 9 inert ingredients. A second list of 

chemicals identified for EDSP screening was published on June 14, 2013,10 and includes some 

pesticides scheduled for registration review and chemicals found in water. 1-MCP is not among the 

group of pesticide active ingredients on the lists to be screened under the EDSP. 

The EPA, as part of this Preliminary Work Plan, believes that 1-MCP is a substance that would not 

likely produce any effect in humans similar to an effect produced by a naturally occurring estrogenic 

substance. As such, pursuant to Section 408(p)(4), the EPA will determine in the future whether it can 

exempt 1-MCP from the requirements of the Section 408(p) EDSP. In the event the EPA does determine 

to exempt this substance from the EDSP, an order will be issued. 

For further information on the status of the EDSP, the policies and procedures, the lists of chemicals, 

future lists, the test guidelines and the Tier 1 screening battery, please visit our website at 

http://www.epa.gov/endo/. 

G. Incidents

From April 22, 1999 (the first date of 1-MCP pesticide registration), through September 2, 2014, OPP’s 

Incident Data System (IDS) contains six reported human incidents consisting of headache, dizziness and 

nausea by applicators while using pesticide products containing 1-MCP as the active ingredient; in two 

of the incidences, the situation was confounded by work conditions consisting of heat stress, dehydration 

and fatigue. In all incidences, the symptoms dissipated within one day. 

A search of OPP’s Environmental Incident Information System (EIIS) conducted on September 3, 2014, 

revealed no incidents associated with 1-MCP; this database contains information dating back to the 

1970s. The EPA will consider any incident data or comments submitted in response to this Preliminary 

Work Plan. 

H. Timeline 

Below is the projected timeline for Registration Review case 0670: 1-MCP: 

 

10 See http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPPT-2009-0477-0074 for the second list of chemicals. 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

15

Activities Estimated Month/Year 

 Opening the Docket 

 Open Docket and 60-Day Public Comment Period for March 2015 

 Close Public Comment Period May 2015 

 Case Development 

 Issue Final Work Plan September 2015 

 Issue Data Call-in N/A 

 Data Submission N/A 

 Open 30-Day Public Comment Period for Draft Risk Assessments N/A 

 Close Public Comment Period N/A 

 Registration Review Decision 

 Open 60-Day Public Comment Period for Proposed Registration Review 

 Decision 

December 2015 

 Close Public Comment Period February 2016 

 Final Decision June 2016 

*Estimated Total (years) 1 year and 3 months 

* This schedule is subject to revision should unforeseen issues arise during the registration review process. In the event an 

issue arises, such as the failure to acquire an EDSP exemption, an amended Final Work Plan will be issued at that time that 

will set forth a new timeline and, if applicable, any new data requirements will be included in the amended document and a 

Data Call-In Notice will be issued. 

I. Guidance for Commenters

 

The public is invited to comment on the EPA’s PWP for 1-MCP and rationale. The areas below highlight 

topics of special interest to the EPA where comments, information and data, or reference to sources of 

additional information could be of particular use. The EPA will consider all comments, as well as any 

additional information or data provided in a timely manner, prior to issuing a Final Work Plan for this 

case. 

i. Environmental Justice 

The EPA seeks to achieve environmental justice, the fair treatment and meaningful involvement of all 

people, regardless of race, color, national origin, or income, in the development, implementation, and 

enforcement of environmental laws, regulations, and policies. To help address potential environmental 

justice issues, the EPA seeks information on any groups or segments of the population who, as a result of 

their location, cultural practices, or other factors, may have unusually high exposure to the pesticidal use 

of 1-MCP, compared to the general population. Please comment if you are aware of any 

subpopulations that may have atypical, unusually high exposure compared to the general 

population. 

ii. Water Quality 

 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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16

1-MCP is not identified as a cause of impairment for any water bodies listed as impaired under section 

303(d) of the Clean Water Act.11 In addition, no Total Maximum Daily Loads (TMDLs) have been 

developed for 1-MCP.12 More information on impaired water bodies and TMDLs can be found on EPA’s 

website.13 The EPA invites submission of water quality data for this pesticide. To the extent possible, 

data should conform to the quality standards in Appendix A of the Office of Pesticide Program’s (OPP) 

Standard Operating Procedure: Inclusion of Impaired Water Body and Other Water Quality Data in 

Registration Review Risk Assessment and Management Process14 to ensure they can be used 

quantitatively or qualitatively in pesticide risk assessments. 

iii. Trade Irritants 

Through the Registration Review process, the EPA intends to solicit information on trade irritants and, to 

the extent feasible, take steps toward facilitating irritant resolution. The EPA will work to harmonize 

tolerance and international Maximum Residue Limits (MRLs), and, if necessary, may modify tolerance 

levels to do so. Growers and other stakeholders are asked to comment on any trade irritant issues 

resulting from lack of MRLs or disparities between U.S. tolerances and MRLs in key export markets, 

providing as much specificity as possible regarding the nature of the concern. 

There are no known MRLs for 1-MCP. Therefore, the EPA does not anticipate that current uses of 1-

MCP will pose concerns as trade irritants. 

iv. Additional Information 

 

Stakeholders are also specifically asked to provide information and data that will assist the EPA in 

refining the risk assessments. The EPA is interested in obtaining the following information regarding 1-

MCP: 

 Confirmation on the following label information: 

- Sites of application 

- Formulations 

- Application methods and equipment 

- Maximum application rates 

- Frequency of application, application intervals, and maximum number of applications 

- Geographic limitations on use

 Use or potential use distribution (e.g., acreage and geographical distribution of relevant use 

sites) 

 Median and 90th percentile reported use rates (lbs active ingredient/1,000 square feet) from 

usage data – national, state, and county 

 Application timing (date of first application and application intervals) – national, state, and 

county 

 Usage/use information for nonagricultural uses 

 Typical application interval (days) 

 

11 Based on information provided at http://water.epa.gov/lawsregs/lawsguidance/cwa/tmdl/index.cfm. 12 Based on information provided at http://iaspub.epa.gov/apex/waters/f?p=ASKWATERS:DOC_SEARCH:0::::: 13 See http://water.epa.gov/lawsregs/lawsguidance/cwa/tmdl/index.cfm. 14 This document can be found at http://www.epa.gov/pesticides/registration-review2/water_quality_sop.htm. 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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17

 State or local use restrictions 

 Monitoring data 

Foreign technical registrants not listed above who supply technical 1-MCP to the US market 

 J. Next Steps 

 

After the 60-day comment period closes, the EPA will review and respond to any comments received in 

a timely manner, then issue a Final Work Plan for 1-MCP. 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

18

II. BIBLIOGRAPHY 

A. Studies Supporting Registration Review of 1-MCP 

MRID Citation Receipt 

Date 

44464700 Biotechnologies for Horticulture, Inc. (1997) Submission of Toxicity and Product Chemistry Data 

in Support of the Application for Registration of MCP. Transmittal of 11 Studies. 

11-Dec1997 

44464701 Kourelis, B. (1997) Manufacturing Process for Methylcyclopropene (MCP). Unpublished study 

prepared by BioTechnologies for Horticulture, Inc. 20 p. 

11-Dec1997 

44464702 Kourelis, B. (1997) Preliminary Analysis of Methylcyclopropene (MCP). Unpublished study 

prepared by BioTechnologies for Horticulture, Inc. 15 p. 

11-Dec1997 

44464703 Daly, J. (1997) Analytical Method for Certified Limits of Methylcyclopropene (MCP). 

Unpublished study prepared by BioTechnologies for Horticulture, Inc. 14 p. 

11-Dec1997 

44464704 Pfeifer, R. (1996) Acute Oral Toxicity Study (in Rats): Methylcyclopropene (MCP): Final Report: 

Lab Project Number: 96G-2085. Unpublished study prepared by Toxikon Corp. 30 p. 

11-Dec1997 

44464705 

Pfeifer, R. (1997) Acute Dermal Toxicity Study (Single Exposure) (in Rabbits): 

Methylcyclopropene (MCP): Final Report: Lab Project Number: 96G-2086: JRM/VIVO/002-

96/000. Unpublished study prepared by Toxikon Corp. 34 p. 

11-Dec1997 

44464706 Bennick, J. (1997) Acute Inhalation Toxicity Study in Rats: EthylBloc (MCP): Final Report: Lab 

Project Number: 3333-97: S9-FF81-3. Unpublished study prepared by Stillmeadow, Inc. 16 p. 

11-Dec1997 

44464707 Prezioso, J. (1996) Primary Eye Irritation Study (in Rabbits): MCP: Final Report: Lab Project 

Number: 96G-2089: JRC/VIVO/005-96/000. Unpublished study prepared by Toxikon Corp. 39 p. 

11-Dec1997 

44464708 Pfeifer, R. (1996) Primary Dermal Irritation--FIFRA (in Rabbits): MCP: Final Report: Lab Project 

Number: 96G-2088: JRM/VIVO/004-96/000. Unpublished study prepared by Toxikon Corp. 33 p. 

11-Dec1997 

44464709 

Prezioso, J. (1996) Salmonella Typhimurium Reverse Mutation Assay--FIFRA: MCP: Final 

Report: Lab Project Number: 96G-2139: JRM/VITRO/001-96/000. Unpublished study prepared by 

Toxikon Corp. 36 p. 

11-Dec1997 

44464710 

Cifone, M. (1997) Mutagenicity Test on MCP in the L5178Y TK +/-Mouse Lymphoma Forward 

Mutation Assay: Final Report: Lab Project Number: 18384-0-431: COVANCE 18384-0-431. 

Unpublished study prepared by Covance Labs., Inc. 29 p. 

11-Dec1997 

44464711 

Ivett, J. (1997) Mutagenicity Test on MCP in an in vivo Mouse Micronucleus Assay: Final Report: 

Lab Project Number: 18384-0-455: COVANCE 18384-0-455. Unpublished study prepared by 

Covance Labs., Inc. 42 p. 

11-Dec1997 

44517000 Biotechnologies for Horticulture, Inc. (1997) Submission of Product Chemistry and Toxicity Data 

in Support of the Application for Registration of EthylBloc. Transmittal of 6 Studies. 

18-Mar1997 

44517001 Daly, J. (1997) Product Identity and Disclosure of Ingredients for 1-Methylcyclopropene (MCP). 

Unpublished study prepared by Biotechnologies for Horticulture, Inc. 59 p. 

18-Mar1997 

44517002 

Daly, J. (1997) Discussion of the Formation of Unintentional Ingredients in Ethylbloc: 

Methylcyclopropene (MCP). Unpublished study prepared by Biotechnologies for Horticulture, Inc. 

17 p. 

18-Mar1997 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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19

44517003 Daly, J. (1997) Certification of Ingredient Limits for EthylBloc. Unpublished study prepared by 

Biotechnologies for Horticulture, Inc. 4 p. 

18-Mar1997 

44517004 

Bennick, J. (1997) Product Chemistry for Non-Combustible End-Use Solids: EthylBloc (MCP): 

Lab Project Number: 3454-97: JRM/VIVO/001-96/000: S9-FD63/EUS. Unpublished study 

prepared by Stillmeadow, Inc. 30 p. 

18-Mar1997 

44517005 Pfeifer, R. (1996) Buehler Sensitization Test (in Guinea Pigs): Final Report: (MCP): Lab Project 

Number: 96G-2087: JRM/VIVO/003-96/000. Unpublished study prepared by Toxikon Corp. 42 p. 

18-Mar1997 

44517006 Daly, J. (1997) Hypersensitivity Incidents Report for Methylcyclopropene (MCP). Unpublished 

study prepared by Biotechnologies for Horticulture, Inc. 4 p. 

18-Mar1997 

44567600 Biotechnologies for Horticulture Inc. (1998) Submission of Product Chemistry Data in Support of 

the Application for Registration of EthylBloc. Transmittal of 1 Study. 

28-May1998 

44567601 

Roberts, A. (1998) Physical and Chemical Properties of EthylBloc-Part II. Unpublished study 

prepared by Biotechnologies for Horticulture, Inc. 4 p. {830.6302, 830.6303, 830.6304, 830.7200, 

83063.14, 830.6316, 830.6320} 

28-May1998 

44735400 Biotechnologies for Horticulture (1999) Submission of Product Chemistry Data in Support of the 

Application for Registration of EthylBloc. Transmittal of 1 Study. 

19-Jan1999 

44735401 

Roberts, A. (1999) Manufacturing Process and Certified Limits for EthylBloc--Supplemental Data. 

Unpublished study prepared by Biotechnologies for Horticulture, Inc. 10 p. {OPPTS 885.1200, 

885.1500} 

19-Jan1999 

44991400 Biotechnologies for Horticulture, Inc. (1999) Submission of Product Chemistry Data in Support of 

the Registration of Ethylbloc. Transmittal of 1 Study. 

10-Dec1999 

44991401 Roberts, A. (1999) Supplemental Product Chemistry for EthylBloc. Unpublished study prepared by 

Biotechnologies for Horticulture, Inc. 30 p. {OPPTS 830.1700, 830.1800} 

10-Dec1999 

45089600 

BioTechnologies for Horticulture (2000) Submission of Product Chemistry, Toxicity, Residue 

Chemistry, and Risk and Exposure Assessment Data in Support of the Registration of EthylBloc 

Technology, and the Petition For Tolerance of 1-Methylcyclopropene in/on Food Commodities. 

Transmittal of 1 Study. 

12-Apr2000 

45089601 

Longacre, S. (2000) An Assessment of the Risk Criteria Established in the Food Quality Protection 

Act (FQPA) Supporting an Exemption from the Requirement of Tolerances for BioTechnologies 

for Horticulture, Inc. Pesticide Petition Unassigned (1-Methylcyclopropene on Post-Harvest Fruits 

and Vegetables): Lab Project Number: AGREG-00-01. Unpublished study prepared by 

Biotechnologies for Horticulture, Inc. 10 p. 

12-Apr2000 

45317100 Rohm and Haas Company (2001) Submission of Exposure Data in Support of the Registration of 1-

Methylcyclopropene. Transmittal of 1 Study. 

01-Feb2001 

45317101 

Shade, W. (2001) 1-Methylcyclopropene (1-MCP): Industrial Source Complex (ISCST3) 

Dispersion Modeling of Air Emissions from Controlled Atmosphere Facilities: Lab Project 

Number: 00R-1066. Unpublished study prepared by Rohm and Haas Company. 10 p. 

01-Feb2001 

45380300 

Rohm and Haas Company (2001) Submission of Toxicity, Residue Chemistry and Risk Assessment 

and Exposure Data in Support of the Registration of EthylBloc Technology and the Petition for 

Tolerance of EthylBloc Technology on Harvested Fruit and Vegetables. Transmittal of 9 Studies. 

12-Apr2001 

45380301 

Ferguson, J.; Craig, L.; Bernacki, H. (2001) 1-Methylcyclopropene: Acute Inhalation Toxicity 

Study in Rats: Lab Project Number: 00P-180: 00R-180. Unpublished study prepared by Rohm and 

Haas Company. 26 p. {OPPTS 870.1300} 

12-Apr2001 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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20

45380302 

O'Neill, P.; Frederick, C. (2001) 1-Methylcyclopropene Vapor Released from 1-

Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% a.i.): Salmonella typhimurium Gene 

Mutation Assay: Lab Project Number: 00R-193A. Unpublished study prepared by Rohm and Haas 

Company. 93 p. 

12-Apr2001 

45380303 

Cifone, M. (2001) 1-Methylcyclopropene Vapor Released from 1-Methylcyclopropene AlphaCyclodextrin Complex (3.3% a.i.): CHO HGPRT Forward Mutation Assay with a Confirmatory 

Assay and Duplicate Cultures: Final Report: Lab Project Number: 21917-0-435 OECD: 00P-195: 

00RC-195. Unpublished study prepared by Covance Laboratories, Inc. 60 p. 

12-Apr2001 

45380304 

Murli, H. (2001) 1-Methylcyclopropene Vapor Released from 1-Methylcyclopropene AlphaCyclodextrin Complex (3.3% a.i.): Chromosomal Abberations in Cultured Human Peripheral Blood 

Lymphocytes: Final Report: Lab Project Number: 21917-0-449OECD: 00P-194: 00RC-194. 

Unpublished study prepared by Covance Laboratories Inc. 66 p. 

12-Apr2001 

45380305 

Sames, J.; Frederick, C. (2001) 1-Methylcyclopropene: Micronucleus Assay in CD-1 Mouse Bone 

Marrow Cells: Lab Project Number: 00R-232. Unpublished study prepared by Rohm and Haas 

Company. 43 p. 

12-Apr2001 

45380306 

Ferguson, J.; Craig, L. (2001) 1-Methylcyclopropene: Two-Week Inhalation Range-Finding Study 

in Female Rats: Lab Project Number: 00P-183A: 00R-183A. Unpublished study prepared by Rohm 

and Haas Company. 34 p. 

12-Apr2001 

45380307 

Longacre, S.; Hazelton, G. (2001) 1-Methylcyclopropene (1-MCP): Dietary and Worker Risk 

Assessments: Lab Project Number: AGREG-01-01. Unpublished study prepared by Rohm and 

Haas Company and AgroFresh, Inc. 14 p. 

12-Apr2001 

45380308 

Parno, J.; Craig, L.; Eberly, S. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% 

a.i.): Acute Oral Toxicity Study in Male and Female Rats: Lab Project Number: 00P-199: 00R-199. 

Unpublished study prepared by Rohm and Haas Company. 26 p. {OPPTS 870.1100} 

12-Apr2001 

45380309 

Verona, D. (2001) (Carbon-14)-1-Methylcyclopropene (1-MCP): Preliminary Result of (Carbon14)-Residues in Apples: Lab Projec1 Number: TR34-01-30. Unpublished study prepared by Rohm 

and Haas Company. 22 p. 

12-Apr2001 

45458600 Rohm and Haas Company (2001) Submission of Product Chemistry and Toxicity Data in Support 

of the Application for Registration of AgroFresh Technology. Transmittal of 8 Studies. 

03-Jul2001 

45458601 

Crawford, J. (2001) Product Chemistry Series Group A: Product Identity, Composition, and 

Analysis of 1-MCP Formulation: Lab Project Number: APR-01-068: APR-01-068: 29P-00-078. 

Unpublished study prepared by Rohm and Haas Company. 296 p. {OPPTS 830.1550, 830.1600, 

830.1620, 830.1670, 830.1700, 830.1750, 830.1800} 

03-Jul2001 

45458602 

Hu, W. (2001) GLP Validation of Test Method TM-2000-245-01: Lab Project Number: 29P-00-

080: APR-01-017: TM-2000-245-01. Unpublished study prepared by Rohm and Haas Company. 43 

p. {OPPTS 830.1800} 

03-Jul2001 

45458603 

Crawford, J. (2001) Product Chemistry Series 830 Group B: Physical and Chemical Characteristics 

of 1-MCP Formulation: Lab Project Number: APR-01-069: 794046: APR-01-019. Unpublished 

study prepared by RCC, Ltd. and Rohm and Haas Company. 370 p. {OPPTS 830.6302, 830.6303, 

830.6304, 830.6314, 830.6315, 830.6316, 830.6317, 830.6319, 830.6320, 830.6321, 830.7000, 

830.7050, 830.7100, 830.7200, 830.7220, 830.7200, 830.7250, 830.7570, 830.7950} 

03-Jul2001 

45458604 

Parno, J.; Craig, L.; Eberly, S. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% 

a.i.) Acute Dermal Toxicity Study in Male and Female Rats: Lab Project Number: 00P-200: 00R200. Unpublished study prepared by Rohm and Haas Company. 19 p. {OPPTS 870.1200} 

03-Jul2001 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

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45458605 

Parno, J.; Craig, L.; Eberly, S. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% 

a.i.) Skin Irritation Study in Rabbits: Lab Project Number: 00P-201: 00R-201. Unpublished study 

prepared by Rohm and Haas Company. 17 p. {OPPTS 870.2500} 

03-Jul2001 

45458606 

Parno, J.; Craig, L.; Eberly, S. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% 

a.i.) Eye Irritation Study in Rabbits: Lab Project Number: 00P-202: 00R-202. Unpublished study 

prepared by Rohm and Haas Company. 18 p. {OPPTS 870.2400} 

03-Jul2001 

45458607 

Parno, J.; Anderson, D.; Danberry, T. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex 

(3.3% a.i.): Dermal Sensitization Study in Guinea Pigs Maximization Test: Lab Project Number: 

00P-203: 00R-203. Unpublished study prepared by Rohm and Haas Company. 62 p. {OPPTS 

870.2600} 

03-Jul2001 

45458608 

Wood, S.; Craig, L.; Bernacki, H. et al. (2001) 1-Methylcyclopropene: Inhalation (Whole-Body) 

Developmental Toxicity Study in Rats: Lab Project Number: 00P-181: 00R-181. Unpublished 

study prepared by Rohm and Haas Company. 248 p. {OPPTS 870.3700} 

03-Jul2001 

45609000 

AgroFresh, Inc. (2001) Submission of Toxicity, Residue, Risk and Exposure Data in Support of the 

Amended Registration of EthylBloc Technology, the Application for Registration of SmartFresh 

and the Petition for Exemption from Tolerance for 1-MCP on All Food Commodities. Transmittal 

of 4 Studies. 

14-Dec2001 

45609001 

Ferguson, J.; Anderson, D.; Bernacki, H. (2001) 1-Methylcyclopropene: Three-Month Inhalation 

(Whole-Body) Toxicity Study in Rats: Lab Project Number: 00P-183: 00R-183: Unpublished study 

prepared by Rohm and Haas Company. 729 p. {OPPTS 870.3465} 

14-Dec2001 

45609002 

Verona, D. (2001) (Carbon 14)-1-Methylcyclopropene (1-MCP) Apple Residue Study: Lab Project 

Number: 34P-01-04: AF-01-141: 1507.03. Unpublished study prepared by AgroFresh, Inc. 351 p. 

{OPPTS 860.1000, 860.1500} 

14-Dec2001 

45609003 

Snyder, B. (2001) Determination of 3-Chloro-2-Methylpropene Concentration in Chamber Treated 

with 1-Methylcyclopropene: Lab Project Number: AF-P-01-01: AF-01-143: AF-P-01-01B. 

Unpublished study prepared by Rohm and Haas Company. 112 p. 

14-Dec2001 

45609004 Hazelton, G. (2001) 1-Methylcyclopropene (1-MCP): Assessment of Worker and Consumer Risk: 

Lab Project Number: 01R-1071. Unpublished study prepared by Rohm and Haas Company. 16 p. 

14-Dec2001 

45843900 AgroFresh, Inc. (2003) Submission of Product Chemistry and Toxicity Data in Support of the 

Application for Registration of SmartFresh SmartTabs. Transmittal of 2 Studies. 

21-Jan2003 

45843901 

Jacobson, R. (2002) Product Chemistry Series 830 Group A: Product Identity, Composition and 

Analysis of SmartFresh Tablet Formulation: Lab Project Number: AGROFRESH-02-054: AF-02-

054: APR-01-017. Unpublished study prepared by AgroFresh Inc.; a Rohm and Haas Company. 51 

p. {OPPTS 830.1550, 830.1600, 830.1620, 830.1650, 830.1700, 830.1750, 830.1800} 

21-Jan2003 

45843902 

Cerven, D. (2002) Acute Oral Toxicity/LD 50 in Rats: SmartFresh Tablets: Lab Project Number: 

MB 02-10029.01: 1000R: 02P-010. Unpublished study prepared by MB Research Laboratories. 13 

p. {OPPTS 870.1100} 

21-Jan2003 

45884100 AgroFresh Inc. (2003) Submission of Product Chemistry Data in Support of the Application for 

Registration of SmartFresh SmartTabs. Transmittal of 2 Studies. 

17-Jan2003 

45884101 

Jacobson, R.; Verona, D. (2002) Product Chemistry Series 830 Group B: Physical and Chemical 

Characteristics of SmartFresh Tablet Formulation: Lab Project Number: AGROFRESH-02-059: 

842968: AF-02-041. Unpublished study prepared by RCC Ltd. and AgroFresh Inc. 235 p. {OPPTS 

830.6302, 830.6303, 830.6304, 830.6314, 830.6316, 830.6317, 830.6320, 830.7000, 830.7300} 

17-Jan2003 
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45884102 

Jacobson, R. (2002) Description and Composition of the SmartFresh SmartTabs Formulation and 

Activator Kit: Lab Project Number: AGROFRESH 02-089: 02-089. Unpublished study prepared by 

AgroFresh Inc. 13 p. 

17-Jan2003 

47024900 

Agro Fresh Inc. (2006) Submission of Product Chemistry, Residue, Exposure and Risk, Toxicity 

and Environmental Fate Data in Support of the Application for Registration of AFxRD-038. 

Transmittal of 28 Studies. 

26-Dec2006 

47024907 Tognucci, A. (2002) Determination of the UV/VIS Absorption Spectra of 1-MCP. Project Number: 

823645, AF/01/186. Unpublished study prepared by RCC Umweltchemie Ag. 44 p. 

26-Dec2006 

47024912 

Schaefer, E.; Siddiqui, A. (2001) 1 Methylcyclopropene (1-MCP): An Evaluation of Ready 

Biodegradability Using the Die-Away Test Method. Project Number: 129E/103, 01RC/0006. 

Unpublished study prepared by Wildlife International Ltd. 60 p. 

26-Dec2006 

47024914 

Drottar, K.; Kendall, T.; Krueger, H. (2001) 1-Methylcyclopropene: A 48 Hour Static Acute 

Immobilization Test with the Cladoceran (Daphnia Magna). Project Number: 129A/184A, 

01RC/0002. Unpublished study prepared by Rohm and Haas Co. 65 p. 

26-Dec2006 

47024915 

Drottar, K.; Kendall, T.; Krueger, H. (2001) 1-Methylcyclopropene: A-96 House Static-Renewal 

Acute Toxicity Test with the Rainbow Trout (Oncorhynchus mykiss). Project Number: 129A/183, 

01RC/0001. Unpublished study prepared by Rohm and Haas Co. 69 p. 

26-Dec2006 

47024917 

Gallagher, S.; Beavers, J. (2006) AFxRD-038: An Acute Oral Toxicity Study with the Northern 

Bobwhite. Project Number: 129/185, 05RC/071. Unpublished study prepared by Rohm and Haas 

Co. 51 p. 

26-Dec2006 

47024918 Gallagher, S.; Beavers, J. (2006) AFxRD-038: A Dietary LC50 Study with the Northern Bobwhite. 

Project Number: 129/184, 05RC/072. Unpublished study prepared by Rohm and Haas Co. 87 p. 

26-Dec2006 

47024919 Gallagher, S.; Martin, K.; Beavers, J. (2006) AFxRD-038: A Dietary LC50 Study with the Mallard. 

Project Number: 129/188, 06RC/130. Unpublished study prepared by Rohm and Haas Co. 87 p. 

26-Dec2006 

47024920 

Porch, J.; Krueger, H. (2001) 1-Methylcyclopropene Alpha-Cyclodextrin Complex (3.3% A.I.): An 

Acute Toxicity Study with the Honey Bee. Project Number: 129/178, 01RC/0097. Unpublished 

study prepared by Wildlife International, Ltd. 50 p. 

26-Dec2006 

47024921 Carbone, J. (2006) 1-Methylcyclopropene: Rationale for Waiver of OPPTS 850.4225 Seedling 

Emergence. Project Number: 06R/1052. Unpublished study prepared by Rohm and Haas Co. 7 p. 

26-Dec2006 

47024922 Carbone, J. (2006) 1-Methylcyclopropene: Rationale for Waiver of OPPTS 850.4250 Vegetative 

Vigor. Project Number: 06R/1053. Unpublished study prepared by Rohm and Haas Co. 6 p. 

26-Dec2006 

47024923 

Krueger, H.; Desjardins, D.; Kendall, T. (2006) Final Report: AFxRD-038: A 96 Hour Toxicity 

Test with the Freshwater Alga (Pseudokirchneriella subcapitata). Project Number: 12A/197B, 

05RC/070. Unpublished study prepared by Wildlife International, Ltd. 74 p. 

26-Dec2006 

47024924 

Drottar, K.; Kendall, T.; Krueger, H. (2001) 1-Methylcyclopropene: A 96 Hour Toxicity Test with 

the Freshwater Alga (Selenastrus capricornutum). Project Number: 129A/185, 01RC/0003. 

Unpublished study prepared by Wildlife International, Ltd. 78 p. 

26-Dec2006 

47024928 

Moore, G. (2007) AFxRD 038: Acute Oral Toxicity Up and Down Procedure in Rats. Project 

Number: 18521, 05RC/064, P320/UDP/R/AND/H. Unpublished study prepared by Product Safety 

Laboratories. 16 p. 

26-Dec2006 

47024929 

Moore, G. (2006) AFxRD 038: Acute Dermal Toxicity Study in Rats - Limit Test. Project Number: 

18522, 05RC/065, P322/R/AND/H. Unpublished study prepared by Product Safety Laboratories. 

16 p. 

26-Dec2006 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47024930 Moore, G. (2006) AFxRD 038: Primary Eye Irritation Study in Rabbits. Project Number: 18523, 

05RC/067, 051020/1R. Unpublished study prepared by Product Safety Laboratories. 15 p. 

26-Dec2006 

47024931 Moore, G. (2006) AFxRD 038: Primary Skin Irritation Study in Rabbits. Project Number: 18524, 

05RC/066, P326/R/AND/H. Unpublished study prepared by Product Safety Laboratories. 16 p. 

26-Dec2006 

47024932 

Nave, V. (2006) Dermal Sensitization Potential of AFxRD 038: Rationale to Use Surrogate 

Toxicology Data. Project Number: 06R/1048. Unpublished study prepared by Rohm and Haas Co. 

7 p. 

26-Dec2006 

47024933 

Nave, V. (2006) Acute Inhalation Potential of AFxR-038: Rationale to Use Surrogate Toxicology 

Data: 1 Methycyclpropene. Project Number: 06R/1011. Unpublished study prepared by Rohm and 

Haas Co. 7 p. 

26-Dec2006 

47024934 

Ferguson, J. (2001) 1-Methylcyclopropene: Two-Week Inhalation Range-Finding Study in Male 

Rats. Project Number: 00R/183B, 00P/183B. Unpublished study prepared by Rohm and Haas Co. 

30 p. 

26-Dec2006 

47024938 Tognucci, A. (2002) Hydrolysis Determination of 1-MCP at Different pH Values. Project Number: 

830790, AF/01/188. Unpublished study prepared by RCC Ltd. 39 p. 

26-Dec2006 

47024941 

Nienstedt, K. (2002) Final Report: 1-Methylcyclopropene: Toxicity Test with the Parasitic Wasp, 

Aphidius rhopalosiphi (Hymenoptera: Braconidae). Project Number: 1007/078/270, 1RC/0283. 

Unpublished study prepared by Rohm and Haas Co. and Springborn Laboratories (Europe) Ag. 70 

p. 

26-Dec2006 

47024942 Tognucci, A. (2006) Determination of the Flammability of 1-MCP Formulation. Project Number: 

823713, AF/01/153. Unpublished study prepared by RCC Ltd. 27 p. 

26-Dec2006 

47024944 

Nienstedt, K.; Milligan, D. (2002) Final Report: 1-Methylcyclopropene: A 14-Day Acute Toxicity 

Test with the Earthworm Eisenia fetida. Project Number: 01RC/0284, 1007/078/630. Unpublished 

study prepared by Springborn Laboratories (Europe) Ag. 89 p. 

26-Dec2006 

47024946 

Nienstedt, K. (2006) Amended Final Report: AFxRD-038: Laboratory Acute Toxicity Test with the 

Parasitic Wasp Aphidius Phopalosiphi (Hymenoptera: Braconidae). Project Number: 06RC/193, 

1007/080/270. Unpublished study prepared by Springborn Laboratories (Europe) Ag. 40 p. 

26-Dec2006 

47024947 

Nienstedt, K. (2006) Amended Final Report: AFxRD-038: Laboratory Acute Toxicity Test with 

Poecilus cupreus L. (Coleoptera: Carabidae). Project Number: 06RC/195, 1007/080/267. 

Unpublished study prepared by Springborn Laboratories (Europe) Ag. 41 p. 

26-Dec2006 

47024949 Carbone, J. (2006) Pre-Harvest of 1-Methylcycpropene on Agriculture Crops: Environmental Risk 

Assessment. Project Number: 06R/1056. Unpublished study prepared by Rohm and Haas Co. 46 p. 

26-Dec2006 

47024950 

Nave, V. (2006) 1-Methylcyclopropene: Rationale for Waiver of OPPTS 880.3550 

Immunotoxicity. Project Number: 06R/1050. Unpublished study prepared by Rohm and Haas Co. 7 

p. 

26-Dec2006 

47024954 

Hazelton, G.; Nave, V.; Shade, W. (2006) Pre-Harvest Use of 1-Methylcyclopropene on 

Agricultural Crops: Toxicology Summary and Assessment of Worker, Bystander and Consumer 

Risks. Project Number: 06R/1055. Unpublished study prepared by Rohm and Haas Co. 133 p. 

26-Dec2006 

47088600 

AgroFresh Inc. (2007) Submission of Product Chemistry, Residue, Fate and Toxicity Data in 

Support of the Application for Registration of AFxRD-038 and the Petition for Tolerance of 1-

Methylcyclopropene (1-MCP). Transmittal of 20 Studies. 

26-Mar2007 

47088601 Weissenfeld, M. (2006) Determination of Colour, Physical State and Odour of AFxRD-038. Project 

Number: A39701, AF/05/032. Unpublished study prepared by RCC Umweltchemie Ag. 23 p. 

26-Mar2007 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47088602 

Kurilla, K. (2006) Accelerated Storage Stability of 1-Methylcyclopropene Formulation AFxRD038. Project Number: AF/06/077, AF/P/06/010. Unpublished study prepared by Agro Fresh Inc. 

and Rohm and Haas Co. 15 p. 

26-Mar2007 

47088603 Weissenfeld, M. (2006) pH-Determination of an Aqueous Solution of AFxRD-038. Project 

Number: A39712, AF/05/033. Unpublished study prepared by RCC Umweltchemie Ag. 24 p. 

26-Mar2007 

47088604 Weissenfeld, M. (2006) Determination of the UV/Vis Absorption Spectrum of AFxRD-038. Project 

Number: A39723, AF/05/034. Unpublished study prepared by RCC Umweltchemie Ag. 33 p. 

26-Mar2007 

47088605 

Weissenfeld, M. (2006) Determination of the Melting Point / Melting Range and the Boiling Point / 

Boiling Range of AFxRD-038. Project Number: A39745, AF/05/036. Unpublished study prepared 

by RCC Umweltchemie Ag. 37 p. 

26-Mar2007 

47088606 Weissenfeld, M. (2006) Determination of the Bulk Density of AFxRD-038. Project Number: 

A39734, AF/05/035. Unpublished study prepared by RCC Umweltchemie Ag. 24 p. 

26-Mar2007 

47088607 Weissenfeld, M. (2006) Determination of the Particle Size Distribution of AFxRD-038. Project 

Number: A39756, AF/05/037. Unpublished study prepared by RCC Umweltchemie Ag. 29 p. 

26-Mar2007 

47088608 Weissenfeld, M. (2006) Determination of the Water Solubility of AFxRD-038. Project Number: 

A71763, AF/06/031. Unpublished study prepared by RCC Umweltchemie Ag. 27 p. 

26-Mar2007 

47088609 

Sutherland, C.; Kendall, T.; Krueger, H. (2006) A 48-Hour Static Acute Immobilization Test with 

the Cladoceran (Daphnia magna): AFxRD-038. Project Number: 129A/195A, 05RC/069. 

Unpublished study prepared by Rohm and Haas Co. and Wildlife International, Ltd. 62 p. 

26-Mar2007 

47088610 

Sutherland, C.; Krueger, H.; Kendall, T. (2006) A 96-Hour Static- Renewal Acute Toxicity Test 

with the Rainbow Trout (Oncorhynchus mykiss): AFxRD-038. Project Number: 129A/196, 

05RC/068. Unpublished study prepared by Rohm and Haas Co. and Wildlife International, Ltd. 63 

p. 

26-Mar2007 

47088611 Mamouni, A. (2006) [14-Carbon]-1-Methylcyclopropene: Residues in Maize. Project Number: 

A73225, AF/06/079. Unpublished study prepared by RCC Umweltchemie Ag. 90 p. 

26-Mar2007 

47088612 Rosenwald, J. (2006) [14-Carbon]-1-Methylcyclopropene: Residue in Apple. Project Number: 

A98515, AF/06/080. Unpublished study prepared by RCC Umweltchemie Ag. 71 p. 

26-Mar2007 

47088613 Weissenfeld, M. (2006) Calculation of the Vapour Pressure of AFxRD-038. Project Number: 

A39778, AF/06/001. Unpublished study prepared by RCC Umweltchemie Ag. 13 p. 

26-Mar2007 

47088614 Tognucci, A. (2002) Determination of the Vapour Pressure of 1-MCP. Project Number: 823601, 

AF/01/190. Unpublished study prepared by RCC Umweltchemie Ag. 33 p. 

26-Mar2007 

47088615 Tognucci, A. (2002) Determination of the Water Solubility of 1-MCP. Project Number: 830801, 

AF/01/191. Unpublished study prepared by RCC Umweltchemie Ag. 34 p. 

26-Mar2007 

47088616 

Hazelton, G.; Swenson, R. (2007) [14-Carbon]-1-Methylcyclopropene: Toxicokinetic Study in 

Rats: Amended Final Report. Project Number: 01R/004E. Unpublished study prepared by Rohm 

and Haas Co. 151 p. 

26-Mar2007 

47088617 

Lampe, K.; Neinstedt, K. (2002) 1-Methylcyclopropene: Laboratory Toxicity Test with the 

Predatory Mite, Typhlodromus pyri Scheuten (Acari: Phytoseiidae): Final Report. Project Number: 

01RC/0282, 1007/078/268. Unpublished study prepared by Rohm and Haas Co. and Springborn 

Laboratories (Europe) Ag. 86 p. 

26-Mar2007 

47088618 

Esdaile, D. (2005) 1-Methylcyclopropene: Structure-Activity Hazard Identification Screen Using 

Derek: Final Report. Project Number: 05RC/1008. Unpublished study prepared by LAB 

International Research Centre Hungary Ltd. 19 p. 

26-Mar2007 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47088619 

Porch, J.; Krueger, H.; Martin, K. (2007) AFxRD-038: A Toxicity Test to Determine the Effects of 

the Test Substance on Vegetative Vigor of Ten Species of Plants: Final Report. Project Number: 

129/191, 06RC/212. Unpublished study prepared by Wildlife International, Ltd.. 165 p. 

26-Mar2007 

47088620 

Porch, J.; Krueger, H.; Martin, K. (2007) AFxRD-038: A Toxicity Test to Determine the Effects of 

the Test Substance on Seedling Emergence of Ten Species of Plants: Final Report. Project Number: 

129/190, 06RC/213. Unpublished study prepared by Wildlife International, Ltd. 138 p. 

26-Mar2007 

47108200 Agrofresh Inc. (2007) Submission of Product Chemistry and Environmental Fate Data in Support 

of the Application for Registration of AFxRD-038. Transmittal of 8 studies. 

16-Apr2007 

47108202 

Mallipudi, M. (2006) GLP Characterization of Five Representative Lots of AFxRD-038 

Formulation. Project Number: AF/P/06/09, AF/06/083. Unpublished study prepared by Agrofresh, 

Inc. 16 p. 

16-Apr2007 

47108203 Verona, D. (2007) Environmental Fate of 1-MCP when Applied Pre-Harvest to Tomato Plants: 

Final Report. Project Number: AF/06/084. Unpublished study prepared by Agrofresh, Inc. 128 p. 

16-Apr2007 

47108204 Mamouni, A. (2006) (carbon-14)-1-Methylcyclopropene: Leaching in Four Soils. Project Number: 

A79053, AF/06/081. Unpublished study prepared by RCC Umweltchemie Ag. 88 p. 

16-Apr2007 

47108205 Kostansek, E. (2007) Product Chemistry AFxRD-038 Formulation. Project Number: AF/06/10. 

Unpublished study prepared by Agrofresh, Inc. 37 p. 

16-Apr2007 

47108206 Rodriguez, J. (2001) Atmospheric Fate of 1-Methylcyclopropene. Unpublished Study Prepared by 

Division of Marine and Atmospheric Science, Univ. of Miami, Project Number: 01RC/1017. 26 p. 

16-Apr2007 

47108207 

Hynes, A. (2001) A Laser Photolysis-Laser Induced Fluorecence Determination of the Rate 

Coefficient for the Reaction of the Hydroxyl Radical with 1- Methylcyclopropene. Prepared by 

Division of Marine and Atmospheric Chemistry at the Univ. of Miami. Project Number: 

01RC/1016. 27 p. 

16-Apr2007 

47108208 

Verona, D.; Mallipudi, M; Kurilla, K (2007) GLP Validation of the GC/FID Method for the 

Determination of 1 MCP in Smart Fresh and AFxRD-038 Formulations using cis-2-Butene as a 

Calibration/Internal Standard. Project Number: AF/06/078, AF/P/06/13. Unpublished study 

prepared by Agrofresh, Inc. 56 p. 

16-Apr2007 

47286100 AgroFresh, Inc. (2007) Submission of Product Chemistry Data in Support of the Application for 

Registration of AFXRD-038. Transmittal of 2 Studies. 

20-Nov2007 

47286101 Espino, P. (2000) Process Safety Test Result and Interpretation for Methylcyclopropene. Project 

Number: RO2685AR. Unpublished study prepared by Chilworth Technology, Inc. 37 p. 

20-Nov2007 

47286102 

Weisel, L. (2003) Physical & Chemical Characterization of 3.3% 1-Methylcycloprene (1-MCP) 

Formulation: Storage Stability and Corrosion Characteristics after 18 Months & 24 Months at 

Room Temperature. Project Number: 713P/00/12, 03/013. Unpublished study prepared by Rohm 

and Haas Co. 127 p. 

20-Nov2007 

47465300 AgroFresh, Inc. (2008) Submission of Product Chemistry Data in Support of the Registration of 

AFxRD-038. Transmittal of 1 Study. 

02-Jul2008 

47465301 

Kurilla, K.; Verona, D. (2008) Interim Report: A 24 Month Study of Storage Stability and 

Corrosion Characteristics of an AFxRD-038 Formulation at 25 Degrees Celsius. Project Number: 

AF/P/07/01, AF/08/074, CIS/CTR/01. Unpublished study prepared by Agrofresh, Inc. 23 p. 

02-Jul2008 

47515000 AgroFresh, Inc. (2008) Submission of Residue Data in Support of the Amended Registration of 

AFxRD-038. Transmittal of 3 Studies. 

13-Aug2008 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47515001 

Rosenwald, J. (2008) (Carbon 14)-1-Methylcyclopropene: Characterization of Residues in Maize 

Grown Under Field Conditions in Switzerland. Project Number: B25784, AF/07/064. Unpublished 

study prepared by RCC, Ltd. 73 p. 

13-Aug2008 

47515002 

Mallipudi, N. (2007) Residues and Characterization of [Carbon 14] 1-Methylcycloprene-Derived 

Residues in Soybean under Field Conditions. Project Number: AF/P/07/03. Unpublished study 

prepared by Xenobiotic, Inc., Access Research and Consulting, Inc. and Research for Hire. 173 p. 

13-Aug2008 

47515003 

Verona, D. (2008) [Carbon 14]-1-Methylcyclopropene (1-MCP) Residue in Cotton under Field 

Conditions: Determination of TRR and Characterization of 1-MCP Residues in Cotton. Project 

Number: AF/P/07/05, AF/08/064. Unpublished study prepared by AgroFresh, Inc., Xenobiotic, Inc. 

and Access Research and Consulting, Inc. 132 p. 

13-Aug2008 

47554100 AgroFresh, Inc. (2008) Submission of Product Chemistry, Toxicity and Exposure and Risk Data in 

Support of the Application for Registration of AF-600. Transmittal of 20 Studies. 

29-Sep2008 

47554101 

Smith, S.; Kurilla, K. (2008) GLP Characterization of Five Representative Batches of AF-600 

Formulation. Project Number: AF/P/08/12, AF/08/079, AF/08/078. Unpublished study prepared by 

Agrofresh, Inc. 29 p. 

29-Sep2008 

47554102 Weissenfeld, M. (2008) AF-600: Determination of Color, Physical State and Odor: Final Report. 

Project Number: B84014, AF/08/024. Unpublished study prepared by RCC, Ltd. 31 p. 

29-Sep2008 

47554103 Kurilla, K. (2008) Accelerated Storage Stability of 1-Methylcyclopropene Formulation AF-600. 

Project Number: AF/08/085, AF/P/08/13. Unpublished study prepared by Agrofresh, Inc. 18 p. 

29-Sep2008 

47554104 Weissenfeld, M. (2008) AF-600: pH-Determination: Final Report. Project Number: B83992, 

AF/08/23. Unpublished study prepared by RCC, Ltd. 33 p. 

29-Sep2008 

47554105 

Cerven, D. (2008) Acute Oral Toxicity - Up and Down Procedure (UDP): AF-600: (Rat). Project 

Number: 08P/044, 08RC/044, MB/08/16870/01. Unpublished study prepared by MB Research 

Laboratories. 14 p. 

29-Sep2008 

47554106 

Cerven, D. (2008) Acute Dermal Toxicity/LD50 in Rats: AF-600. Project Number: 

MB/08/16870/02, 2100S/02, 08P/045. Unpublished study prepared by MB Research Laboratories. 

16 p. 

29-Sep2008 

47554107 

DiDonato, L. (2008) Primary Dermal Irritation/Corrosion in Rabbits: AF-600. Project Number: 

MB/08/16870/03, 4130R/H/02, 08P/046. Unpublished study prepared by MB Research 

Laboratories. 17 p. 

29-Sep2008 

47554108 Nave, V. (2008) Acute Inhalation Potential of AF-600: Rationale to Use Ingredient Data. Project 

Number: 08R/1013. Unpublished study prepared by Rohm and Haas Co. 10 p. 

29-Sep2008 

47554109 Hall, D. (2008) Acute Eye Irritation in Rabbits: AF-600. Project Number: MB/08/16870/04, 

1200R/02, 08P/047. Unpublished study prepared by MB Research Laboratories. 19 p. 

29-Sep2008 

47554110 

Hall, D. (2008) Guinea Pig Maximization Test (Magnusson-Kligman). Project Number: 

MB/08/16870/06, 2160R/02, 08P/048. Unpublished study prepared by MB Research Laboratories. 

58 p. 

29-Sep2008 

47554111 Weissenfeld, M. (2008) AF-600: Determination of the Viscosity: Final Report. Project Number: 

B84060, AF/08/027. Unpublished study prepared by RCC, Ltd. 43 p. 

29-Sep2008 

47554112 Weissenfeld, M. (2008) AF-600: Determination of the Auto-Ignition Temperature: Final Report. 

Project Number: B84058, AF/08/026. Unpublished study prepared by: RCC, Ltd. 37 p. 

29-Sep2008 

47554113 Weissenfeld, M. (2008) AF-600: Determination of the Flash Point: Final Report. Project Number: 

B84047, AF/08/025. Unpublished study prepared by RCC, Ltd. 33 p. 

29-Sep2008 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47554114 Weissenfeld, M. (2008) AF-600: Determination of the Relative Density: Final Report. Project 

Number: B84071, AF/08/028. Unpublished study prepared by RCC, Ltd. 36 p. 

29-Sep2008 

47554115 

Kurilla, K.; Verona, D. (2008) GLP Validation of the GC/FID Method for the Determination of 1-

MCP in AF-600 Formulations Using Cis-2-Butene as a Calibration/Internal Standard. Project 

Number: AF/08/051, AF/P/08/04. Unpublished study prepared by Agrofresh, Inc. 34 p. 

29-Sep2008 

47554116 

Nave, V. (2008) AF-600: Rationale for Waiver of Acute Oral Toxicity Study with the Northern 

Bobwhite Quail. Project Number: 08R/1043. Unpublished study prepared by Rohm and Haas Co. 8 

p. 

29-Sep2008 

47554117 

Nave, V. (2008) AF-600: Rationale for Waiver of Acute Toxicity Test with the Rainbow Trout 

(Oncorhynchus mykiss). Project Number: 08R/1044. Unpublished study prepared by Rohm and 

Haas Co. 8 p. 

29-Sep2008 

47554118 

Nave, V. (2008) AF-600: Rationale for Waiver of Acute Toxicity Immobilization Test with the 

Cladoceran (Daphnia magna). Project Number: 08R/1045. Unpublished study prepared by Rohm 

and Haas Co. 8 p. 

29-Sep2008 

47554119 Kostansek, E. (2008) Product Chemistry: AF-600. Project Number: AGROFRESH/08/093, 

AF/08/051, AF/P/08/04. Unpublished study prepared by Agrofresh, Inc. 165 p. 

29-Sep2008 

47554120 

Driver, J.; Hewitt, A.; Ross, J. (2008) Evaluation of Potential Handler and Bystander Exposures 

and Health Risks Associated with Pre-Harvest Aerial Application of 1-MCP. Project Number: 

IS/C/02/001/08, 08RC/1004. Unpublished study prepared by Rohm and Haas Co. 48 p. 

29-Sep2008 

47611800 AgroFresh (2008) Submission of Product Chemistry Data in Support of the Amended Registration 

of EthylBloc Sachet. Transmittal of 1 Study. 

28-Nov2008 

47611801 Ranly, P. (2008) EthylBloc Sachet: Alternate Manufacturing Process. Project Number: AF/08/106. 

Unpublished study prepared by Agrofresh, Inc. 26 p. 

28-Nov2008 

47690300 AgroFresh, Inc. (2009) Submission of Product Chemistry Data in Support of the Application for 

Registration of AF-600. Transmittal of 1 Study. 

02-Mar2009 

47690301 Kostansek, E. (2009) Additional Product Chemistry Information for AF-600. Project Number: 

AGROFRESH/08/093B, B84036, B84025. Unpublished study prepared by Agrofresh, Inc. 47 p. 

02-Mar2009 

47876400 Agro Fresh, Inc. (2009) Submission of Product Chemistry, Toxicity, Fate and Residue Data in 

Support of the Application for Registration of A17492F. Transmittal of 16 Studies. 

01-Oct2009 

47876401 

Verona, D. (2009) A17492F Formulation: Product Chemistry Group A. Project Number: 

AF/09/070, T001982/09, TK0001951. Unpublished study prepared by Syngenta Crop Protection. 

116 p. 

01-Oct2009 

47876402 

Verona, D. (2009) A17492F Formulation: Product Chemistry Group B. Project Number: 

AF/09/073, TK0001953, HT09/226. Unpublished study prepared by Syngenta Crop Protection, Inc. 

32 p. 

01-Oct2009 

47876403 

Mallipudi, N. (2009) Comparative Residue Study of the 1-Methylcyclopropene (1-MCP) 

Formulations AFxRD-038 and A17492F: Residue and Characterization of [Carbon 14], 1-MCP 

Residues in Soybean under Field Conditions: Interim Report. Project Number: 27893, AF/09/067, 

09049. Unpublished study prepared by Eurofins/Product Safety Laboratories and Xenobiotic, Inc. 

64 p. 

01-Oct2009 

47876404 

Kumar, S. (2009) 1-Methylcyclopropene XX: Acute Oral Toxicity Study (Up-and-Down 

Procedure) In Rats: Amended Final Report. Project Number: G6801, G6801/AOR. Unpublished 

study prepared by Advinus Therapeutics Private, Limited. 49 p. 

01-Oct2009 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

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47876405 

Kumar, S. (2009) 1-Methylcyclopropene XX: Acute Dermal Toxicity Study in Rats: Amended 

Final Report. Project Number: G6802, G6802/ADR. Unpublished study prepared by Advinus 

Therapeutics Private, Limited. 48 p. 

01-Oct2009 

47876406 

Kumar, S. (2009) 1-Methylcyclopropene XX: Acute Eye Irritation Study in Rabbits: Amended 

Final Report. Project Number: G6803, G6803/EYE. Unpublished study prepared by Advinus 

Therapeutics Private, Limited. 53 p. 

01-Oct2009 

47876407 

Kumar, M. (2009) 1-Methylcyclopropene XX: Acute Dermal Irritation Study in Rabbits: Amended 

Final Report. Project Number: G6804, G6804/SKIN. Unpublished study prepared by Advinus 

Therapeutics Private, Limited. 47 p. 

01-Oct2009 

47876408 

Nave, V. (2009) Acute Inhalation Potential of Formulation A17492F (1-Methylcyclopropene XX): 

Rationale to Use Surrogate Toxicology Data. Project Number: 09R/1029. Unpublished study 

prepared by Agrofresh, Inc. 10 p. 

01-Oct2009 

47876409 

Nave, V. (2009) Dermal Sensitization Potential of Formulation A17492F (1-Methylcyclopropene 

XX): Rationale to Use Surrogate Toxicology Data. Project Number: 09R/1030. Unpublished study 

prepared by Agrofresh, Inc. 10 p. 

01-Oct2009 

47876410 

Nave, V. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

OPPTS 880.3550 Immunotoxicity. Project Number: 09R/1031. Unpublished study prepared by 

Agrofresh, Inc. 12 p. 

01-Oct2009 

47876411 

Carbone, J. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

Acute Oral Toxicity Study with the Northern Bobwhite Quail. Project Number: 09R/1033. 

Unpublished study prepared by Agrofresh, Inc. 9 p. 

01-Oct2009 

47876412 

Carbone, J. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

Dietary Toxicity Study with the Northern Bobwhite Quail. Project Number: 09R/1034. 

Unpublished study prepared by Agrofresh, Inc. 9 p. 

01-Oct2009 

47876413 

Carbone, J. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

Acute Toxicity Test with the Rainbow Trout (Oncorhynchus mykiss). Project Number: 09R/1035. 

Unpublished study prepared by Agrofresh, Inc. 11 p. 

01-Oct2009 

47876414 

Carbone, J. (2009) A17492F (1-Methylcyclopropene XX): Rationale for Waiver of Acute Toxicity 

Immobilization Test with the Cladoceran (Daphnia magna). Project Number: 09R/1036. 

Unpublished study prepared by Agrofresh, Inc. 11 p. 

01-Oct2009 

47876415 

Carbone, J. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

Seedling Emergence Test. Project Number: 09R/1037. Unpublished study prepared by Agrofresh, 

Inc. 9 p. 

01-Oct2009 

47876416 

Carbone, J. (2009) Formulation A17492F (1-Methylcyclopropene XX): Rationale for Waiver of 

Vegetative Vigor Test. Project Number: 09R/1038. Unpublished study prepared by Agrofresh, Inc. 

9 p. 

01-Oct2009 

47913700 AgroFresh, Inc. (2009) Submission of Product Chemistry, Toxicity and Fate Data in Support of the 

Application for Registration of AF-625. Transmittal of 15 Studies. 

16-Nov2009 

47913701 Kostansek, E.; Verona, D. (2009) AF-625 Formulation: Product Chemistry Group A. Project 

Number: AGROFRESH/09/068, 5945. Unpublished study prepared by Agrofresh, Inc. 159 p. 

16-Nov2009 

47913702 

Verona, D. (2009) AF-625 Formulation: Product Chemistry Group B. Project Number: 

AGROFRESH/09/076, AF/09/064, AF/P/09/08. Unpublished study prepared by Agrofresh, Inc. 

and Harlan Laboratories, Ltd. 203 p. 

16-Nov2009 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

29

47913703 

Ravi, G. (2009) AF-625: Acute Oral Toxicity Study (Up-and-Down Procedure) in Rats: Final 

Report. Project Number: G6851, G6851/AOR. Unpublished study prepared by Advinus 

Therapeutics Private Limited. 48 p. 

16-Nov2009 

47913704 

Ravi, G. (2009) AF-625: Acute Dermal Toxicity Study in Rats: Final Report. Project Number: 

G6852/ADR, G6852, SP/G6852. Unpublished study prepared by Advinus Therapeutics Private 

Limited. 48 p. 

16-Nov2009 

47913705 

Kumar, S. (2009) AF-625: Acute Eye Irritation Study in Rabbits: Final Report. Project Number: 

G6853, G6853/EYE, SP/G6853. Unpublished study prepared by Advinus Therapeutics Private 

Limited. 50 p. 

16-Nov2009 

47913706 

Kumar, M. (2009) AF-625: Acute Dermal Irritation Study in Rabbits: Final Report. Project 

Number: G6854, G6854/SKIN, SP/G6854. Unpublished study prepared by Advinus Therapeutics 

Private Limited. 47 p. 

16-Nov2009 

47913707 Nave, V. (2009) Acute Inhalation Potential of Formulation AF-625: Rationale to Use Surrogate 

Toxicology Data. Project Number: 09R/1042. Unpublished study prepared by Agrofresh, Inc. 10 p. 

16-Nov2009 

47913708 Nave, V. (2009) Dermal Sensitization Potential of Formulation AF-625: Rationale to Use Surrogate 

Toxicology Data. Project Number: 09R/1043. Unpublished study prepared by Agrofresh, Inc. 10 p. 

16-Nov2009 

47913709 Nave, V. (2009) Formulation AF-625: Rationale for Waiver of OPPTS 880.3550 Immunotoxicity. 

Project Number: 09R/1044. Unpublished study prepared by Agrofresh, Inc. 12 p. 

16-Nov2009 

47913710 

Carbone, J. (2009) AF-625: Rationale for Waiver of Acute Oral Toxicity Study with the Northern 

Bobwhite Quail: Final Report. Project Number: 09R/1045. Unpublished study prepared by 

Agrofresh, Inc. 10 p. 

16-Nov2009 

47913711 

Carbone, J. (2009) AF-625: Rationale for Waiver of Dietary Toxicity Study with the Northern 

Bobwhite Quail: Final Report. Project Number: 09R/1046. Unpublished study prepared by 

Agrofresh, Inc. 10 p. 

16-Nov2009 

47913712 

Carbone, J. (2009) AF-625: Rationale for Waiver of Acute Toxicity Test with the Rainbow Trout 

(Oncorhynchus mykiss): Final Report. Project Number: 09R/1047. Unpublished study prepared by 

Agrofresh, Inc. 10 p. 

16-Nov2009 

47913713 

Carbone, J. (2009) AF-625: Rationale for Waiver of Acute Toxicity Immobilization Test with the 

Cladoceran (Daphnia magna): Final Report. Project Number: 09R/1048. Unpublished study 

prepared by Agrofresh, Inc. 10 p. 

16-Nov2009 

47913714 Carbone, J. (2009) AF-625: Rationale for Waiver of Seedling Energence Test: Final Report. Project 

Number: 09R/1049. Unpublished study prepared by Agrofresh, Inc. 10 p. 

16-Nov2009 

47913715 Carbone, J. (2009) AF-625: Rationale for Waiver of Vegetative Vigor Test: Final Report. Project 

Number: 09R/1050; 09/1050. Unpublished study prepared by Agrofresh, Inc. 10 p. 

16-Nov2009 

48087000 Agro Fresh, Inc. (2010) Submission of Toxicity Data in Support of the Application for Registration 

of AF-625. Transmittal of 2 Studies. 

07-May2010 

48087001 

Nave, V. (2009) Acute Inhalation Potential of Formulation AF-625: Rationale to Use Surrogate 

Toxicology Data. Project Number: 09R/1042. Unpublished study prepared by Agro Fresh, Inc. 10 

p. 

07-May2010 

48087002 Carbone, J. (2010) AF-625: Rationale for Waiver of Non-Target Insect Toxicity Test. Project 

Number: 10R/1017. Unpublished study prepared by Agro Fresh, Inc. 9 p. 

07-May2010 

48567300 AgroFresh, Inc. (2011) Submission of Product Chemistry and Toxicity Data in Support of the 

Application for Registration of SmartFresh ProTabs. Transmittal of 12 Studies. 

09-Aug2011 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

30

48567301 Durando, J. (2011) AF-777 Powder: 1-Methylcyclopropene: Acute Oral Toxicity Up and Down 

Procedure in Rats. Unpublished study prepared by Eurofins/Product Safety Laboratories. 28p. 

09-Aug2011 

48567302 

Durando, J. (2011) AF-777 Powder: 1-Methylcyclopropene: Acute Dermal Toxicity Study in Rats. 

Project Number: 31418 100293 P322/DOW. Unpublished study prepared by Eurofins/Product 

Safety Laboratories. 26p. 

09-Aug2011 

48567303 

Durando, J. (2011) AF-777 Powder: 1-Methylcyclopropene: Acute Inhalation Toxicity Study in 

Rats. Project Number: 31419 100315 P330/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 37p. 

09-Aug2011 

48567304 

Durando, J. (2011) AF-777 Powder: 1-Methylcyclopropene: Primary Eye Irritation Study in 

Rabbits. Project Number: 31420 100295 P324/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 29p. 

09-Aug2011 

48567305 

Durando, J. (2011) AF-777: 1-Methylcyclopropene: Primary Skin Irritation Study in Rabbits. 

Project Number: 31421 100294 P326/DOW. Unpublished study prepared by Eurofins/Product 

Safety Laboratories. 29p. 

09-Aug2011 

48567306 

Durando, J. (2011) AF-777 Powder: 1-Methylcyclopropene: Local Lymph Node Assay (LLNA) in 

Mice. Project Number: 31422 100296 P327/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 40p. 

09-Aug2011 

48567307 

Carbone, J. (2011) AF-777: Rationale for Waiver of Acute Oral Toxicity Study with the Northern 

Bobwhite Quail. Project Number: NS000037. Unpublished study prepared by The Dow Chemical 

Co. 10p. 

09-Aug2011 

48567308 

Carbone, J. (2011) AF-777: Rationale for Waiver of Acute Toxicity Test with the Rainbow Trout 

(Oncorhynchus mykiss). Project Number: NS000039. Unpublished study prepared by The Dow 

Chemical Co. 10p. 

09-Aug2011 

48567309 

Carbone, J. (2011) AF-777: Rationale for Waiver of Acute Toxicity Immobilization Test with the 

Cladoceran (Daphnia magna). Project Number: NS000040. Unpublished study prepared by The 

Dow Chemical Co. 10p. 

09-Aug2011 

48567310 

Carbone, J. (2011) AF-777: Rationale for Waiver of Dietary Toxicity Study with the Northern 

Bobwhite Quail. Project Number: NS000041. Unpublished study prepared by The Dow Chemical 

Co. 10p. 

09-Aug2011 

48567311 

Jacobson, R.; Verona, D. (2011) AF-777 1-Methylcyclopropene Formulation: Product Chemistry 

Group A. Project Number: AGROFRESH/11/097, AF/11/097. Unpublished study prepared by 

Agrofresh, Inc., and Wildlife International, Ltd. 252p. 

09-Aug2011 

48567312 

Verona, D. (2011) Product Chemistry Series 830 Group B: Physical and Chemical Characteristics 

of AF-777 1-Methylcyclopropene Formulation. Project Number: AGROFRESH/11/098, D15766. 

Unpublished study prepared by Agrofresh, Inc., and Harlan Laboratories, Ltd. 232p. 

09-Aug2011 

48628300 Agri Fresh, Inc. (2011) Submission of Product Chemistry, Toxicity and Residue Data in Support of 

the Application for Registration of AF-2005. Transmittal of 19 Studies. 

11-Oct2011 

48628301 

Jacobson, R.; Kurilla, K. (2011) AF-2005 1-Methylcyclopropene Formulation Product Chemistry 

Group A. Project Number: AGROFRESH/11/112. Unpublished study prepared by Agrofresh, Inc. 

222p. 

11-Oct2011 

48628302 

Kurilla, K. (2011) Product Chemistry Series 830 Group B: Physical and Chemical Characteristics 

of AF-2005 1-Methylcyclopropene Formulation. Project Number: AGROFRESH/11/117. 

Unpublished study prepared by Harlan Laboratories, Ltd. and Agrofresh, Inc. 64p. 

11-Oct2011 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

31

48628303 

Minderhout, T.; Martin, K.; Gallagher, S.; et al. (2011) AF-2005: Formulation of 1-

Methylcyclopropene (1-MCP): A 48-Hour Static Acute Immobilization Test with the Cladoceran 

(Daphnia magna): Final Report. Project Number: 103A/165, 110057, 100297. Unpublished study 

prepared by Wildlife International, Ltd. 64p. 

11-Oct2011 

48628304 

Minderhout, T.; Kendall, T.; Gallagher, S.; et al. (2011) AF-2005: Formulation of 1-

Methylcyclopropene (1-MCP): A 96-Hour Static-Renewal Acute Toxicity Test with the Rainbow 

Trout (Oncorhynchus mykiss): Final Report. Project Number: 103A/166, 110058. Unpublished 

study prepared by Wildlife International, Ltd. 68p. 

11-Oct2011 

48628305 

Hubbard, P.; Beavers, J. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-MCP): An 

Acute Oral Toxicity Study with the Northern Bobwhite. Project Number: 103/448, 110053. 

Unpublished study prepared by Wildlife International, Ltd. 59p. 

11-Oct2011 

48628306 

Hubbard, P.; Martin, K.; Beavers, J. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-

MCP): A Dietary LC50 Study with the Northern Bobwhite. Project Number: 103/446, 110054. 

Unpublished study prepared by Wildlife International, Ltd. 69p. 

11-Oct2011 

48628307 

Sindermann, A.; Porch, J.; Krueger, H. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-

MCP): An Acute Contact Toxicity Study with the Honey Bee: Final Report. Project Number: 

103/445, 100317. Unpublished study prepared by Wildlife International, Ltd. 32p. 

11-Oct2011 

48628308 

Carbone, J. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-MCP): Rationale for 

Waiver of Seedling Emergence Test. Project Number: NS000047. Unpublished study prepared by 

Dow Chemical Company. 10p. 

11-Oct2011 

48628309 

Carbone, J. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-MCP): Rationale for 

Waiver of Vegetative Vigor Test. Project Number: NS000048. Unpublished study prepared by 

Dow Chemical Company. 10p. 

11-Oct2011 

48628310 

Kurilla, K.; Faubion, D.; Jacobson, A.; et al. (2011) Magnitude of Residues of 1-

Methylcyclopropene (1-MCP) on Apples following Application of AF-2005. Project Number: 

AF/P/11/04, AF/11/124. Unpublished study prepared by Agrofresh, Inc. and ExperiCo. 107 p. 

11-Oct2011 

48628311 

Kumar, S. (2011) AF-2005: Acute Oral Toxicity Study (Up-and-Down Procedure) in Rats: Final 

Report. Project Number: G7768, G7768/AOR. Unpublished study prepared by Advinus 

Therapeutics Private Limited. 50p. 

11-Oct2011 

48628312 

Kumar, S. (2009) 1-MCP: Manufacturing Use Product: Acute Oral Toxicity Study (Up-and-Down 

Procedure) in Rats: Amended Final Report. Project Number: G6799, G6799/AOR. Unpublished 

study prepared by Advinus Therapeutics Private Limited. 49p. 

11-Oct2011 

48628313 Kumar, S. (2011) AF-2005: Acute Dermal Toxicity Study in Rats: Final Report. Project Number: 

G7769, G7769/ADR. Unpublished study prepared by Advinus Therapeutics Private Limited. 49p. 

11-Oct2011 

48628314 

Durando, J. (2010) Manufacturing Use Product - HAIP (1-Methylcyclopropene): Acute Inhalation 

Toxicity Study in Rats. Project Number: 30311, 100137, P330/DOW. Unpublished study prepared 

by Eurofins/Product Safety Laboratories. 37p. 

11-Oct2011 

48628315 

Krieger, S.; Mahoney, K. (2011) AF-2005: Acute Liquid Aerosol Inhalation Toxicity Study in 

Crl:CD(SD) Rats. Project Number: 111102. Unpublished study prepared by The Dow Chemical 

Co. 71p. 

11-Oct2011 

48628316 Prakash, P. (2011) AF-2005: Acute Eye Irritation Study in Rabbits: Final Report. Project Number: 

G7919, G7919/EYE. Unpublished study prepared by Advinus Therapeutics Private Limited. 50p. 

11-Oct2011 

48628317 

Prakash, P. (2011) AF-2005: Acute Dermal Irritation Study in Rabbits: Final Report. Project 

Number: G7770, G7770/SKIN. Unpublished study prepared by Advinus Therapeutics Private 

Limited. 50p. 

11-Oct2011 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

32

48628318 

Sulaiman, S. (2011) AF-2005: Local Lymph Node Assay (LLNA) in CBA/Ca Mice: Final Report. 

Project Number: G7772, G7772/LLNA. Unpublished study prepared by Advinus Therapeutics 

Private Limited. 71p. 

11-Oct2011 

48628319 

Durando, J. (2011) AF-2005 without 1-MCP: Local Lymph Node Assay (LLNA) in Mice. Project 

Number: 32615, 110115, P327/DOW. Unpublished study prepared by Eurofins/Product Safety 

Laboratories. 41p. 

11-Oct2011 

48786100 Agro Fresh, Inc. (2012) Submission of Fate Data in Support of the Application for Registration of 

AF-2005. Transmittal of 2 Studies. 

30-Mar2012 

48786101 

Sindermann, A.; Martin, K.; Porch, J. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-

MCP): A Toxicity Test to Determine the Effects of the Test Substance on Seedling Emergence of 

Ten Species of Plants: Final Report. Project Number: 103/449, 110061. Unpublished study 

prepared by Wildlife International, Ltd. 83p. 

30-Mar2012 

48786102 

Sindermann, A.; Porch, J.; Martin, K. (2011) AF-2005: Formulation of 1-Methylcyclopropene (1-

MCP): A Toxicity Test to Determine the Effects of the Test Substance on Vegetative Vigor of Ten 

Species of Plants: Final Report. Project Number: 103/450, 110060. Unpublished study prepared by 

Wildlife International, Ltd. 103p. 

30-Mar2012 

49061100 Agro Fresh, Inc. (2013) Submission of Product Chemistry Data in Support of the Registration of 

Smartfresh Protabs. Transmittal of 1 Study. 

19-Feb2013 

49061101 

Kurilla, K. (2013) Storage Stability and Corrosion Characteristics of AF-777 1-

Methylcyclopropene (Yellow) Formulation (Vector Yellow VEC298-2011 Lot 1): Final Report. 

Project Number: AF/P/11/32, AF/13/005. Unpublished study prepared by Agrofresh, Inc. 25p. 

19-Feb2013 

49183700 AgroFresh, Inc. (2013) Submission of Product Chemistry Data in Support of the Registration of 

AF-2005. Transmittal of 1 Study. 

25-Jul2013 

49183701 

Kurilla, K. (2013) Storage Stability of 0.8% 1-Methylcyclopropene Formulation AF-2005 for 12 

months at 25 Degrees Celsius. Project Number: AF/P/11/30, AF/13/001. Unpublished study 

prepared by Agro Fresh, Inc. 28p. 

25-Jul2013 

49201900 AgroFresh, Inc. (2013) Submission of Toxicity and Product Chemistry Data in Support of the 

Application of Registration of AF-701. Transmittal of 15 Studies. 

26-Aug2013 

49201901 

Jacobson, A. (2013) Formulation AF-701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver Acute Toxicity Immobilization Test with the Cladoceran (Daphnia magna): Final Report. 

Project Number: AF/13/072. Unpublished study prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201902 

Jacobson, A. (2013) Formulation AF/701 (Active Ingredient 1-Methycyclopropene): Rationale for 

Waiver of Acute Toxicity Test with the Rainbow Trout (Oncorhynchus mykiss): Final Report. 

Project Number: AF/13/071. Unpublished study prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201903 

Jacobson, A. (2013) Formulation AF/701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver of Acute Oral Toxicity Study with the Northern Bobwhite Quail: Final Report. Project 

Number: AF/13/070. Unpublished study prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201904 

Jacobson, A. (2013) Formulation AD-701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver of Dietary Toxicity Study with the Northern Bobwhite Quail: Final Report. Project 

Number: AF/13/076. Unpublished study prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201905 

Jacobson, A. (2013) Formulation AF-701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver of Honey Bee (Apis mellifera) Acute Contact Toxicity Test: Final Report. Project Number: 

AF/13/075. Unpublished study prepared by Agrofresh, Inc. 10p. 

26-Aug2013 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

33

49201906 

Jacobson, A. (2013) Formulation AF-701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver of Seedling Emergence Test: Final Report. Project Number: AF/13/073. Unpublished study 

prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201907 

Jacobson, A. (2013) Formulation AF-701 (Active Ingredient 1-Methylcyclopropene): Rationale for 

Waiver of Vegetative Vigor Test: Final Report. Project Number: AF/13/073. Unpublished study 

prepared by Agrofresh, Inc. 10p. 

26-Aug2013 

49201908 

Durando, J. (2010) 1-Methylcyclopropene Formulation AF-701: Acute Oral Toxicity Up and Down 

Procedure in Rats. Project Number: 28867, 090592, P320/UDP/DOW. Unpublished study prepared 

by Eurofins/Product Safety Laboratories. 30p. 

26-Aug2013 

49201909 

Durando, J. (2010) 1-Methylcyclopropene Formulation AF-701: Acute Dermal Toxicity Study in 

Rats. Project Number: 28868, 090593, P322/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 29p. 

26-Aug2013 

49201910 

Merolla, L. (2013) Formulation AF-701 (active ingredient 1-Methycyclopropene): Rationale for 

Waiver of Acute Inhalation Study. Project Number: AF/13/082. Unpublished study prepared by 

Agrofresh, Inc. 9p. 

26-Aug2013 

49201911 

Durando, J. (2010) 1-Methylcyclopropene Formulation AF-701: Primary Eye Irritation Study in 

Rabbits. Project Number: 28869, 090595, P324/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 32p. 

26-Aug2013 

49201912 

Durando, J. (2010) 1-Methylcyclopropene Formulation AF-701: Primary Skin Irritation Study in 

Rabbits. Project Number: 28870, 090594, P326/DOW. Unpublished study prepared by 

Eurofins/Product Safety Laboratories. 31p. 

26-Aug2013 

49201913 

Boverhof, D.; Sosinski, L. (2010) 1-Methylcyclopropene Formulation AF-701: Local Lymph Node 

Assay In CBA/ J Mice. Project Number: 091147. Unpublished study prepared by Dow Chemical, 

USA. 25p. 

26-Aug2013 

49201914 

Guo, I.; Jacobson, A.; Lieberman, E. et al. (2013) AF-701: A 1--Methycyclopropene (1-MCP) 

Formulation Product Chemistry Group A. Project Number: AF/13/068. Unpublished study 

prepared by Agrofresh, Inc. 112p. 

26-Aug2013 

49201915 Guo, I. (2010) 1-Methycyclopropene (1-MCP) AF-701 Formulation Product Chemistry Group B. 

Project Number: AGROFRESH/10/013. Unpublished study prepared by Agrofresh, Inc. 161p. 

26-Aug2013 

49265000 Janssen PMP (2014) Submission of Product Chemistry, Toxicity, Fate, Residue and Efficacy Data 

in Support of the Application for Registration of Fysium. Transmittal of 55 Studies. 

11-Mar2014 

49265001 

Goodwine, W. (2014) Product Chemistry Group A: Product Identity & Composition, and Analysis: 

Fysium (1-MCP). Project Number: PPD/60. Unpublished study prepared by Janssen 

Pharmaceutica. 94p. 

11-Mar2014 

49265002 

Comb, A. (2014) 1-MCP Five Batch Analysis (using five batches of LAg 2011 009 and one 

generator): Final Report. Project Number: AGR/4954, IMB0059, CT003597PCP01. Unpublished 

study prepared by Huntingdon Life Sciences, Ltd. 53p. 

11-Mar2014 

49265003 Comb, A. (2014) 1-MCP Method Validation: Final Report. Project Number: AGR/4958, IMB0063, 

295469. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 95p. 

11-Mar2014 

49265004 

Comb, A. (2014) 1-MCP: Assessment of Yield of Gas Generator at Different Loadings. Project 

Number: AGR/4957, IMB0062, 295469. Unpublished study prepared by Huntingdon Life 

Sciences, Ltd. 30p. 

11-Mar2014 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

34

49265005 

Comb, A. (2014) 1-MCP: Physico-Chemical Properties (Interim Report). Project Number: 

AGR/4959, IMB0064, 295469. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 

46p. 

11-Mar2014 

49265006 

Younis, S. (2013) Regulatory Testing on a Sample of 1-Methycyclopropene: Final Report. Project 

Number: AGR/4944, GLP109415R1V1/2013, GLP/109415. Unpublished study prepared by 

Chilworth Technology. 18p. 

11-Mar2014 

49265007 

Robinson, M. (2013) 1-Methylcyclopropene: Single Dose Toxicity Study by Inhalation 

Administration to Rats: Final Report. Project Number: AGR/4802, IMB0042. Unpublished study 

prepared by Huntingdon Life Sciences, Ltd. 49p. 

11-Mar2014 

49265008 

May, K. (2013) 1-MCP: Bacterial Reverse Mutation Test (Gas Phase): Final Report. Project 

Number: AGR/4803, IMB0043. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 

61p. 

11-Mar2014 

49265009 

Woods, I. (2013) 1-MCP: In Vitro HPRT Mutation Test Using Chinese Hamster Ovary Cells (Gas 

Phase): Final Report. Project Number: AGR/4805, IMB0045. Unpublished study prepared by 

Huntingdon Life Sciences, Ltd. 55p. 

11-Mar2014 

49265010 

Woods, I. (2013) 1-MCP: In Vitro Mammalian Chromosome Aberration Test in Human 

Lymphocytes (Gas Phase): Final Report. Project Number: AGR/4804, IMB0044. Unpublished 

study prepared by Huntingdon Life Sciences, Ltd. 52p. 

11-Mar2014 

49265011 

Goodwine, W. (2014) Product Chemistry Group A: Product Identity, Composition, Analysis - 

Component B (LAG 2011 009). Project Number: PPD/60. Unpublished study prepared by Janssen 

Pharmaceutica. 64p. 

11-Mar2014 

49265012 Comb, A. (2014) LAg 2011 009: Five Batch Analysis: Final Report. Project Number: AGR/4947, 

IMB0055. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 30p. 

11-Mar2014 

49265013 Comb, A. (2014) LAg 0211 009: Method Validation: Final Report. Project Number: AGR/4948, 

IMB0056. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 34p. 

11-Mar2014 

49265014 

Wo, C. (2013) Physical and Chemical Characteristics: Color, Physical State, Odor, 

Oxidation/Reduction, Flashpoint, pH, Viscosity and Density/Relative Density: LAG 2011 009. 

Project Number: AGR/4815, PSL/35789, P804/TGAI. Unpublished study prepared by Product 

Safety Laboratories. 18p. 

11-Mar2014 

49265015 Comb, A. (2014) LAg 2011 009: Physico-Chemical Properties: Final Report. Project Number: 

AGR/4905, IMB0052. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 62p. 

11-Mar2014 

49265016 Comb, A. (2014) LAg 2011 009: Accelerated Storage Stability: Final Report. Project Number: 

AGR/5033, IMB0083. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 27p. 

11-Mar2014 

49265017 Comb, A. (2014) LAg 2011 009: Low Temperature Stability: Final Report. Project Number: 

AGR/5032, IMB0082. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 12p. 

11-Mar2014 

49265018 

Lowe, C. (2013) Acute Oral Toxicity Up and Down Procedure in Rats: LAG 2011 009. Project 

Number: AGR/4809, PSL/35766, P320/UDP. Unpublished study prepared by Product Safety 

Laboratories. 15p. 

11-Mar2014 

49265019 

Lowe, C. (2013) Acute Dermal Toxicity Study in Rats: LAG 2011 009. Project Number: 

AGR/4810, PSL/35767, P322/RAT. Unpublished study prepared by Product Safety Laboratories. 

15p. 

11-Mar2014 

49265020 Lowe, C. (2013) Acute Inhalation Toxicity Study in Rats: LAG 2011 009. Project Number: 

AGR/4811, PSL/35768, P330. Unpublished study prepared by Product Safety Laboratories. 23p. 

11-Mar2014 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

35

49265021 Lowe, C. (2013) Primary Eye Irritation Study in Rabbits: LAG 2011 009. Project Number: 

AGR/4812, PSL/35769, P324. Unpublished study prepared by Product Safety Laboratories. 15p. 

11-Mar2014 

49265022 Lowe, C. (2013) Primary Skin Irritation Study in Rabbits: LAG 2011 009. Project Number: 

AGR/4813, PSL/35770, P326. Unpublished study prepared by Product Safety Laboratories. 15p. 

11-Mar2014 

49265023 Lowe, C. (2013) Local Lymph Node Assay (LLNA) in Mice: LAG 2011 009. Project Number: 

AGR/4814, PSL/35771, P327. Unpublished study prepared by Product Safety Laboratories. 24p. 

11-Mar2014 

49265024 May, K. (2013) Lag 2011 009: Bacterial Reverse Mutation Test: Final Report. Project Number: 

AGR/4800, IMB0040. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 49p. 

11-Mar2014 

49265025 

Woods, I. (2013) Lag 2011 009: In Vitro Mammalian Chromosome Aberration Test in Human 

Lymphocytes: Final Report. Project Number: AGR/4801, IMB0041. Unpublished study prepared 

by Huntingdon Life Sciences, Ltd. 50p. 

11-Mar2014 

49265026 

Goodwine, W. (2014) Product Chemistry Group A: Product Identity, Composition and Analysis: 

Component A (LAG 2011 009). Project Number: PPD/62. Unpublished study prepared by Janssen 

Pharmaceutica. 53p. 

11-Mar2014 

49265027 Comb, A. (2014) LAg 2011 008: Five-Batch Analysis: Final Report. Project Number: AGR/5052, 

IMB0084, 2723/140613. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 21p. 

11-Mar2014 

49265028 Comb, A. (2014) LAg 2011 008: Method Validation: Final Report. Project Number: AGR/5044, 

IMB0077. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 22p. 

11-Mar2014 

49265029 

Wo, C. (2013) Physical & Chemical Characteristics: Color, Physical State, Odor, 

Oxidation/Reduction, Flammability, pH, Viscosity, Density/Relative Density - Janssen Code# LAG 

2011 008. Project Number: AGR/4992, PSL/36385, P801. Unpublished study prepared by Product 

Safety Laboratories. 16p. 

11-Mar2014 

49265030 Comb, A. (2014) LAg 2011 008: Physico-Chemical Properties: Final Report. Project Number: 

AGR/5034, IMB0076. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 23p. 

11-Mar2014 

49265031 Comb, A. (2014) LAg 2011 008: Accelerated Storage Stability: Final Report. Project Number: 

AGR/5042, IMB0079. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 24p. 

11-Mar2014 

49265032 

Comb, A. (2014) LAg 2011 008: Low Temperature Storage Stability: Final Report. Project 

Number: AGR/5041, IMB0078. Unpublished study prepared by Huntingdon Life Sciences, Ltd. 

12p. 

11-Mar2014 

49265033 

Wo, C. (2013) Janssen Code# LAg 2011 008: Physical and Chemical Characteristics: Boiling 

Point. Project Number: AGR/5065, PSL/36810, P801. Unpublished study prepared by Product 

Safety Laboratories. 11p. 

11-Mar2014 

49265034 

Lowe, C. (2013) Acute Oral Toxicity Up and Down Procedure in Rats: Janssen Code# LAg 2011 

008. Project Number: AGR/4995, PSL/36386, P320/UDP. Unpublished study prepared by Product 

Safety Laboratories. 15p. 

11-Mar2014 

49265035 

Lowe, C. (2013) Acute Dermal Toxicity Study in Rats: Janssen Code# LAg 2011 008. Project 

Number: AGR/4996, PSL/36387, P322/RAT. Unpublished study prepared by Product Safety 

Laboratories. 20p. 

11-Mar2014 

49265036 

Lowe, C. (2013) Acute Dermal Toxicity Study in Rats - Limit Test: Janssen Code# LA g 2011 008. 

Project Number: AGR/5107, PSL/37090, P322/RAT. Unpublished study prepared by Product 

Safety Laboratories. 23p. 

11-Mar2014 

49265037 

Lowe, C. (2013) Janssen Code # LAg 2011 008: Acute Inhalation Toxicity Study in Rats - Limit 

Test. Project Number: AGR/4997, PSL/36388, P330. Unpublished study prepared by Product 

Safety Laboratories. 22p. 

11-Mar2014 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

36

49265038 

Lowe, C. (2013) Janssen Code #LAg 2011 008: Primary Skin Irritation Study in Rabbits. Project 

Number: AGR/4999, PSL/36390, P326. Unpublished study prepared by Product Safety 

Laboratories. 14p. 

11-Mar2014 

49265039 

Lowe, C. (2013) Janssen Code# LAg 2011 008: Local Lymph Node Assay (LLNA) in Mice. 

Project Number: AGR/5000, PSL/36391, P327. Unpublished study prepared by Product Safety 

Laboratories. 23p. 

11-Mar2014 

49265040 Neumans, E. (2013) 1-methylcyclopropene: Physical & Chemical Characteristics: Vapor Pressure. 

Project Number: AGR/5130. Unpublished study prepared by Janssen Pharmaceutica. 7p. 

11-Mar2014 

49265041 

Douahiher, M. (2013) Physical & Chemical Characteristics: Determination of the Melting 

Point/Melting Range of 1-Methycycloprene. Project Number: AGR/5131. Unpublished study 

prepared by Janssen Pharmaceutica. 8p. 

11-Mar2014 

49265042 Warkentien, K. (2014) 1-MCP Physical-Chemical Properties Data Waivers. Project Number: 

FYSIUM/PC/1013. Unpublished study prepared by Lewis & Harrison, LLC. 20p. 

11-Mar2014 

49265043 Warkentien, K. (2014) 1-MCP: Health Effects: Data Waivers. Project Number: 

FYSIUM/TOX/0913. Unpublished study prepared by Lewis & Harrison, LLC. 20p. 

11-Mar2014 

49265044 Warkentien, K. (2014) 1-MCP Ecological Effects Data Waivers. Project Number: 

FYSIUM/ECO/0913. Unpublished study prepared by Lewis & Harrison, LLC. 17p. 

11-Mar2014 

49265045 

Lauwers, D. (2013) Storage Stability Test on Component B (Lag 2011 009) for 14 days 54 

(Degrees Celcius). Project Number: 13024. Unpublished study prepared by Janssen Pharmaceutica. 

31p. 

11-Mar2014 

49265046 Warkentien, K (2014) 1-MCP: Precursor Physical-Chemical Properties Data Waivers. Project 

Number: FYSIUM/PRE/PC/1013. Unpublished study prepared by Lewis and Harrision, LLC. 18p. 

11-Mar2014 

49265047 Warkentien, K. (2014) 1-MCP Precursor Health Effects Data Waivers. Project Number: 

FYSIUM/PRE/TOX/0913. Unpublished study prepared by Lewis & Harrison, LLC. 22p. 

11-Mar2014 

49265048 Warkentien, K. (2014) 1-MCP: Precursor Ecological Effects. Project Number: 

FYSIUM/PRE/ECO/0913. Unpublished study prepared by Lewis & Harrison, LLC. 22p. 

11-Mar2014 

49265049 

Cotterhill, J. (2013) In Silico Assessment of Mammalian Toxicity and Ecotoxicity of Lag 2011 

009. Project Number: AGR/5113, W5ZZ3001/2. Unpublished study prepared by Food and 

Environmental Research Agency (Fera). 61p. 

11-Mar2014 

49265050 Warkentien, K. (2014) 1-MCP Activator Physical-Chemical Properties Data Waivers. Project 

Number: FYSIUM/PC/1013. Unpublished study prepared by Lewis & Harrison, LLC. 21p. 

11-Mar2014 

49265051 Warkentien, K. (2014) 1-MCP Activator Health Effects. Project Number: 

FYSIUM/ACT/TOX/0913. Unpublished study prepared by Lewis & Harrison, LLC. 26p. 

11-Mar2014 

49265052 Warkentien, K. (2014) 1-MCP Activator Ecological Effects. Project Number: 

FYSIUM/ACT/ECO/0913. Unpublished study prepared by Lewis & Harrison, LLC. 22p. 

11-Mar2014 

49265053 

Cotterhill, J. (2013) In Silico Assessment of Mammalian Toxicity and Ecotoxicity of Lag 2011 

008: Final Report. Project Number: AGR/5112, W5ZZ3001/1. Unpublished study prepared by 

Food and Environmental Research Agency (Fera). 39p. 

11-Mar2014 

49265054 Bosselaers, J. (2014) Physical & Chemical Characteristics: Boiling Point: 1-Methylcyclopropene. 

Project Number: AGR/5140. Unpublished study prepared by Janssen Pharmaceutica. 9p. 

11-Mar2014 

49265055 

Neumans, E. (2014) Physical & Chemical Characteristics: Henry''s Law Constant: 1-

methylcyclopropene. Project Number: AGR/5141. Unpublished study prepared by Janssen 

Pharmaceutica. 8p. 

11-Mar2014 
1-Methylcyclopropene EPA-HQ-OPP-2014-0670 

(1-MCP) www.regulations.gov 

 

37

49340500 AgroFresh, Inc. (2014) Submission of Product Chemistry and Toxicity Data in Support of the 

Application for Registration of AF-701. Transmittal of 3 Studies. 

13-Mar2014 

49340501 

Kurilla, K. (2011) Storage Stability of 1-Methylcyclopropene Formulation AF-701 for 12 Months 

at 25(Degree)C. Project Number: AF/P/10/02, AF/11/007, GLP1002. Unpublished study prepared 

by Agrofresh, Inc. 25p. 

13-Mar2014 

49340502 

Minderhout, T.; Siddiqui, A.; Martin, K.; et al. (2014) AF-701: Formulation of 1-

Methylcyclopropene (1-MCP): A 48-Hour Static Acute Immobilization Test with Cladoceran 

(Daphnia magna): Final Report. Project Number: 103A/175, AF/13/091, 

103/102113/DAP/48H1/SUB103. Unpublished study prepared by Wildlife International, Ltd. 64p. 

13-Mar2014 

49340503 

Minderhout, T.; Siddiqui, A.; Martin, K.; et al. (2014) AF-701: Formulation of 1-

Methylcyclopropene (1-MCP): A 96-Hour Static Renewal Acute Toxicity Test with the Rainbow 

Trout (Oncorhynchus mykiss): Final Report. Project Number: 103A/176, AF/13/093, 

103/102113/RBT/96H3/SUB103. Unpublished study prepared by Wildlife International, Ltd. 68p. 

13-Mar2014 

B. U.S. Environmental Protection Agency Regulatory Documents and Risk Assessment 

Memoranda 

U.S, EPA, 2001. Science Review in Support of a Petition (Petition No. 0F06144) from the Requirements 

of a Tolerance on All Food Commodities and Label Amendment for EthylBlocTM (EPA Reg. No. 

071297-1) Containing 0.14% 1-Methylcyclopropene (Chemical No. 224459). From Russell S. 

Jones, Ph.D, through Freshteh Toghrol, Ph.D., to Driss Benmhend. February 21, 2001. 

U.S, EPA, 2010. Science Review in Support of the Registration of A17492F, Containing 1.0% 1 

Methylcyclopropene (1-MCP) As Its Active Ingredient. From Russell S. Jones, Ph.D, to Menyon 

Adams. February 9, 2010. 

U.S. EPA, 2008. Biopesticide Registration Action Document (BRAD) 1- Methylcyclopropene (PC Code 

224459). U.S. Environmental Protection Agency (U.S. EPA), May 30, 2008. Available at: 

http://www.epa.gov/opp00001/chem_search/reg_actions/registration/decision_PC-224459_30-

May-08.pdf